LOCUS       DAOSSW010000001        59803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_566_length_59803_cov_6.561408, whole genome shotgun sequence.
ACCESSION   DAOSSW010000001 DAOSSW010000000
VERSION     DAOSSW010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 59803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59803
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_566_length_59803_cov_6.561408"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PKW26_00005"
     CDS             <1..534
                     /locus_tag="PKW26_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOQ91980.1"
                     /translation="ALDVFASKKNLRLIVADIPSDEDWEYLSVDGGLLVQNRDRALFQ
                     RWEPVTKHEPTPKQIEEMIFGMIVAIFVKSNAILISKDKTAIGIAGGQTNRIWAASQA
                     LERAKQFTDKERLSNAEVLISDAFFPFSDTVEEAAKYGIKAIIQPGGSLRDQESIDAA
                     NKLGIAMIFTGCRHFKH"
     gene            558..1628
                     /locus_tag="PKW26_00010"
     CDS             558..1628
                     /locus_tag="PKW26_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOQ91981.1"
                     /translation="MLFHLASYLATFFGPARLLKSYAVLITLALYLGFFISYFFLPKL
                     YPKLPRDRGRDFTPGSEAAKGKPTGGGAVFISIFVIIAFFFVPLTTLQIAVVILTWIT
                     MILGFLDDKSSLPWGEYRKALFDLLICLVASFILYHLNPKVLNGEALYIWLPFFTQAF
                     AIPQWPFILLSTIILWVSINTTNCTDGVDGLSSTLALIALITMGIIFYFIIGHKDISA
                     YLLVPHLKDGASWAILTFALSGVLMGYLWHNAFPSKVLMGDAGSRALGFYIGTSVIVS
                     GNPFLLLATSSIILLNGGMGLLKVFLLRFFKVRLFQNIRFPLHDHMKDSRQWSSTQVL
                     VKFMILQILITIAMLGLIFKVR"
     gene            1705..3792
                     /locus_tag="PKW26_00015"
     CDS             1705..3792
                     /locus_tag="PKW26_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOQ91982.1"
                     /translation="MSSILDIFGSNVFNDAAMQEYLSIDVYTALKKTIKEGSPLDLRL
                     ANAIAKGMKDWALSKGATHYTHWFQPMTGITAEKHDSFLSRDKNGDAIIDFSGKELIK
                     GEPDGSSFPSGGLRATFEARGYTTWDPTSYAFLKDNTLCIPTAFCSYGGLSLDKKTPL
                     LRSMTALNKQALRIMKLFGTKTSRVISTVGSEQEYFLIDRKLFFKRKDLVFCNRTLFG
                     AHPPKGQELDDHYFGAIKPRIASYMKELDEELWKLGIYAKTKHNETAPAQHELAPIYT
                     ETNIAADHNQLTMEIMKKIAVKHGLTCLLHEKPFKGVNGSGKHNNWSISTDSGINLLD
                     PGDSPSENAQFLLILTAIIKAVDEHQDLLRLSVATAGNDHRLGGHEAPPAIISIFLGE
                     ELTTILESIRDEKPYNKKNAELLTLGVDTLPSFPKDSTDRNRTSPFAFSMNKFEFRSL
                     GASVSISCPNIMLNTIIADAFCQFADVLEKAEDFTSSLNQLIRDNLKKHSRIIFNGNG
                     YDDAWVREAKKRGLLNLKNTAEALPYFLDTKNRLLFTKHKVHTELEMESRYEVLMENY
                     CKVLHIEALTMIDMAKKDIFPSVLKYLHEIANTVNALKTFLPQAKLKGQERIARTLAL
                     LFDQADEQLQSLEKEVGKTKDIEGVTKTATYYAEKIIPAMNALRESCDEMECLTSKEH
                     WPFPSYADLLFRV"
     gene            3797..4492
                     /locus_tag="PKW26_00020"
     CDS             3797..4492
                     /locus_tag="PKW26_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ91983.1"
                     /translation="MIEIIDIKKHYTTKGQEAQALRGITTRIQNGEFSALSGPSGSGK
                     TTLLNLIGCLDSASSGSIFLDGKNLVSLTKQETIKIRQSKIGFIFQSYNLIPVLSAYE
                     NVALALEVQNMFSKTEIKHLVYEMLKNVGLEGYEKRKPSQLSGGQQQRVSVARALVKK
                     PQIVLADEPTANLDSKNAETILHLMKEHNEKQKTTFLFSTHDELVLSFVNRKLSLRDG
                     LLIADSKAQTNGE"
     gene            complement(4479..5741)
                     /locus_tag="PKW26_00025"
     CDS             complement(4479..5741)
                     /locus_tag="PKW26_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOQ91984.1"
                     /translation="MFTIKRQNKNIGQKIGRGLFFTSLIFSLFVFFSCMTSSGLTGLD
                     ETEGKNKSEAAQKARDAANKGFMDEMGEGIGIADIPESLPGRTVKPSQFDEPEIVITQ
                     NINTSTKQPDWVKNTQTSFPSSRYLSAVGSGKSTSEAENAAIASIARIIKQNVSSTLV
                     TTEKDAVSSTGASSNQSTVDEIIETYAVVDALVGVKIAEFWWDKNDIVYALAYVDKQE
                     AAEYYVRKMGENENEIMEKISVAKNSLGQFSSFSLALQALSLAEENEQYIDILYAMDA
                     KTAQSNTFRYGSIVSVKEFSYAVAEAIAIDISVVGDDDGRIGLALSTMLAKYGFKTYI
                     GTSNANYLLDAAVSFVDVPSERNVYVRYIFSANLVDKKTTKILLPYSDNKREGHVHEA
                     GAKERAIQSLVTTIQDEYASFFVDFIRR"
     gene            complement(5729..7054)
                     /locus_tag="PKW26_00030"
     CDS             complement(5729..7054)
                     /locus_tag="PKW26_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ91985.1"
                     /translation="MQGKKLRITFLFFSFFALLFFSSCVTNRHFFNQVDSHVGRGDYQ
                     TAVSMIEKNEDNLYTKKDLVLKSLDKGLLLHYGGDYKTSNKELAYAEEKMFEYFSKSI
                     SQSVQSFIINDTVIDYAGDPYEDIYSNLFMALNYIALNEIDKALVEIRRFDTKQKNLR
                     LNYAQAQNKANQNSLSDAYFGEMSFHNSALARYLSMLLYRAEGNVDAAEIDLRYIKTA
                     FRDQSQLYPFSLPSSLDDELLKLPGLERLNILCFTGLAPIKTEEVIRFYTSDTYYKLA
                     LPIMFKRASRIVSISVAVENEDTGEKHQFRLDPLESIDSIAYDTFRQKQDLLYFKAIA
                     RSIAKSMTTNVLDAASRVDQNGDASGILGPLSIVSLVSNELTEVADLRTSRYFPAKAS
                     VGGIHLAPGRYTVQIQYKDAWGGTIWLETIQGFDLKKNTINLVEGICLQ"
     gene            complement(7064..7678)
                     /locus_tag="PKW26_00035"
     CDS             complement(7064..7678)
                     /locus_tag="PKW26_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HOQ91986.1"
                     /translation="MKKTSIIAIAMMLIVVLSSCVSTTGTQVNRIDARTQTDLSGYWN
                     DTDVRIIADTLVQECVNAPSIVNFIRQYQRPPVVILGSFRNQSDEHLDTSILAKKFEI
                     ALVNSGKVDFVASSMERGELRDERENQQEWSSELSAKALANEVAADFMLIGAVKTIVD
                     SSGADFTRTYFVTAELINIEKNTKVWLGENSSIKKYIRRDAVRW"
     gene            complement(7719..8945)
                     /locus_tag="PKW26_00040"
     CDS             complement(7719..8945)
                     /locus_tag="PKW26_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HOQ91987.1"
                     /translation="MNTSESIYETIDKSKDEMIALQRILTAIPALDPSSGGKGELQKC
                     LALEKWLRENGIENLERFDAPDARADSGIRPNLIATLPGKKSQNLWIMTHLDVVPTGD
                     LSLWDSDPWEMLEKDGKLYGRGTEDNQQGLVSSIFALKALLANKQIPEYTVKLLFVAD
                     EEVGSTYGIQYLLENHALFQKDDLIIIPDGGDSEGLCIEIAEKNILWFQVKTLGKQCH
                     GSRPDEGINAHLAACDLALLLHDMKNHFSAQNSLFEPVYSTFEPTKKEANVPNINTIP
                     GEDSFFMDCRILPQYSLDEVRLEIEKRTKSIKKKYGVEIVVEEKQAVESPATPHDAEV
                     VQVLKKAIQKLRGKEARLIGIGGGTVASYLRNEGLHAAVWSTLDETAHQPNEYCRIEN
                     MIDDAKVLASIMLGNN"
     gene            complement(9003..9968)
                     /gene="galE"
                     /locus_tag="PKW26_00045"
     CDS             complement(9003..9968)
                     /gene="galE"
                     /locus_tag="PKW26_00045"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOQ91988.1"
                     /translation="MKVLVIGGAGYIGSHVVKALMKEKHEVVVFDNLSSGMRENLFTQ
                     NAFIHGDILIQADLDAAFSQGFDAFVHLAAFKAAGESMLIPEKYSINNITGTLNILHA
                     ALAHNCKRMVFSSSAAIFGEPEYLPIDENHPKNPENYYGFTKLEIERIMEWYDRLKGL
                     RYASLRYFNAAGYDIEGEITAKEQNPANLLPVIMEVASGMREKLQVFGTDYETRDGTC
                     IRDYVHVWDLAIAHVKALDYITKEDKSLFVNLGSEVGLSVLEILDAAKKASGKEIPTD
                     FVERRAGDPECLYAVSKKAKDLLGWEAQYSDAKTLVETTWKVYKK"
     gene            complement(10134..11357)
                     /locus_tag="PKW26_00050"
     CDS             complement(10134..11357)
                     /locus_tag="PKW26_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOQ91989.1"
                     /translation="MWIEQLSSVSLILLTLCIMLFSGFLLTRVTKKLRLPNVTGYILA
                     GVLIGPSVFNIIPKQMISNMGFISDIALTFIAFSVGRFFKKEKLKKTGPEVLVITLFE
                     SLFAGVIVTIVMFFVFRLSFAFSLLLGAIATATAPASTIVTIRQYGARGKFVNTLLQV
                     VALDDAVCLIVFSIATALVGHEGGQVASIQSILLTVLYNVLSVGLGFLCGFILSRLMT
                     PTRSEDNRLILTLGLLLGIAGLCAIVHVSPLLACMAFGMGYINLTKDKVLYEQVEKFS
                     PPILSLFFIVSGMNLDLTSFTTYGLIGLTYFFVRILGKYFGAFLGCAVTGTSHKIRNY
                     LGFALVPQAGVAIGLAFLGQRLLPEDSGMMLLSIILSSSVLYELVGPASAKFALHRSG
                     AIAKGQKKKRKKEGT"
     gene            complement(11488..13443)
                     /locus_tag="PKW26_00055"
     CDS             complement(11488..13443)
                     /locus_tag="PKW26_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOQ91990.1"
                     /translation="MSFVQFSKVSLAFGDRDILKDVSINLSRGTKAALAGANGSGKTT
                     LMKVLAGIIHPDSGERAVQKETQISYLPQSGIVHKGRSLAAEADSAFQRGYELEAELE
                     AIGDALVKKPTNTKVLLHRHQEIHEELEQMLWYRRDALVEQILLGLGFERSDFSRRVE
                     EFSGGWQMRIALAKVLLEDPDILLLDEPTNYLDLEARNWLEQFLQNFPGGFLLVSHDR
                     YFLDTTVNEVYEIFSGSLKPYAGNYSKYEKQREVEIEGLLARYAQQQEEIKKLEDFIR
                     RFGAKATKAAQAQERQKMLDKMERIEIPENLKQIRFRFPPAPHSGQIVLDLEGIGKTY
                     DGEKWILKELSLVIEKGERFVIAGRNGAGKTTLLRILSGEDANFEGSFRLGSGVNIGY
                     FSQDNAEKICGEKSILESLEEECPLDLVPKARDLLASFLFRGDDVYKSLDVLSGGEKS
                     RLALLRLLLKPVNLLILDEPTNHLDLHSKDVLLSALSDFGGTILFVSHDRGFIESLAT
                     KVLELKEGKQRVFPGDYRYYLEKIEAENTLSENYVQQKKGEDKAQGKIESKEEKNPGQ
                     LSWEESKRRRSEMRKLEKDLEILLEKIASFEEQLAVLHENLSKPEVYMDGDKSKKIQE
                     EITVIGKKMEDAHLAWEDLETKRESLL"
     gene            complement(13450..14724)
                     /locus_tag="PKW26_00060"
     CDS             complement(13450..14724)
                     /locus_tag="PKW26_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOQ91991.1"
                     /translation="MKAKYTCFFGFILALSFTLFSCSHSLGFGVVLWSLPEEGLSDGD
                     IVPVFIQSNINQVFVIGVPNSDKKIEVPLWQISTPEKKAQAIETAKKYKAYKNIYARV
                     KLDGLPVRYEATNLGRQVYRLKEGEIVKVLFAGEGAPVNGLEGDWLRVLMQDGSVGWC
                     FSYNLSLFDETDDNYVEEEKKDDRFETFAKKTWYPQSYQTMIQNGRINLDEMKVDYGF
                     SINTETKIASLRLKNITKSFPFEDVEKKEDDLYQLTASPLLLRIVSENQIQVTLVGDF
                     GKQEIHAFSTVSSPIRDIIDAEKKRRSFLLQTIINQGPSFSSSNYGLLQFLDEGSFVW
                     TGYSLLSPSLIPSGVGSRGKVSMDYFISNDLSFYYDGVLSFRFDSDQKEIVFLYKLEE
                     TGLRMEFVPENLIRNALISSQSQNPIILFFLK"
     gene            complement(14829..16079)
                     /locus_tag="PKW26_00065"
     CDS             complement(14829..16079)
                     /locus_tag="PKW26_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOQ91992.1"
                     /translation="MKLFLKYRKKAKPALFICVFLLSFLPAVFFAQENLPIERYAIYV
                     ASNDGGKGREILRYAGTDANRLAQTMIDVGGVKVENSNILLDPSKKELDRYLRVLSEK
                     VVKNKSHTKRTEFVFYYSGHSDENALLLGNERYDYVDLKASISAVPSDVHVVMLDSCF
                     SGNFIRAKGGEKQKSFLVDDSTVVQGHAYLSSSSESESSQESDLIQASFFTHALITGL
                     RGAADSSGDNKVSLNELYHYAFNETLLQTERSTIGPQHPSYNITLVGSGDLILTDITD
                     SESIIVFPAEIEGRFFVRTSDGVLVSEVNKQRGSKMSLALNQGYYTIISIIGGLTQQT
                     SVSLRSGATYLLSSRDFVPVQTFSGRPRGGTEEKPDIYTDRYPDINTNRNEIMLIITN
                     AAGISYNVDEDKTFVQESDAWNFR"
     gene            complement(16090..16899)
                     /locus_tag="PKW26_00070"
     CDS             complement(16090..16899)
                     /locus_tag="PKW26_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ91993.1"
                     /translation="MRKKDLYLEQIILGEVLYKNNDFEIKQKEEINAIKKENKAILEK
                     YPAYDMEFAVRSKMLSSKEEKLRGGEKKFRPNETKAFFTSQRIMAIAAMLCLAFLLPI
                     GIQLKNRTETQGQNRVFIEPKGSERAKGFGGKIFVYKKEGSSAVRLANLSKVSEGDVL
                     QVSYVSSGFSYGYIFSVDGNGHCTQHFPEKGKEPGLLTGKGEIALDYAYKLDNAPSFE
                     RFFFISSKDSFSRKNLDAFFNQIDLNARDSIDNLASYLPSALEVHDVFLVK"
     gene            complement(16930..17439)
                     /locus_tag="PKW26_00075"
     CDS             complement(16930..17439)
                     /locus_tag="PKW26_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOQ91994.1"
                     /translation="MNKEEEYLNIEHFTDLYERFGPMVLRRCRFLLKDEEKALDCMQD
                     VFVRILERKERITSVCSSLFYTVATRVCLNKIRYDSVRWAPDIESFITEIEDNYRANH
                     EDIIDASLVLDAIFDETKKSTQDMALMHYVDGFTLEETAKEVGLSVSGVRKRLLILRK
                     KALAKHQEE"
     gene            17562..18098
                     /locus_tag="PKW26_00080"
     CDS             17562..18098
                     /locus_tag="PKW26_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ91995.1"
                     /translation="MDIILHSVTIFAFVLVFFEFFSFFFRKKKALKKLGEKKKNLLAK
                     HSFSFLTLFVLSPMLIGLVYVKKIDFFPSIILYACAILALEIAMRDFTTKKLQGIYEK
                     GFVINNKIILYADIIDFNPFAPKNNDDSDIAEDEQYIGTSRLDIATESKGLIFVDFSS
                     TGEKDQFLDFYKNHVSST"
     gene            complement(18222..18293)
                     /locus_tag="PKW26_00085"
     tRNA            complement(18222..18293)
                     /locus_tag="PKW26_00085"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18259..18261),aa:Cys,seq:gca)
     gene            18367..18813
                     /locus_tag="PKW26_00090"
     CDS             18367..18813
                     /locus_tag="PKW26_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ91996.1"
                     /translation="MFKIEPHIEFASDKNSAYYSLFWSQLEKADKYKITTSVPSVSGL
                     YEIYYEDAEKRLNLLNVAAAWLGGLRSQIRSDIDSDGKSDEVKEILETYSLYYRYTIS
                     PSFDSIKDVAWFLNKTYFKDASTSQHSGRFETIYVKEFAPDKIRWV"
     gene            18871..19809
                     /gene="trxB"
                     /locus_tag="PKW26_00095"
     CDS             18871..19809
                     /gene="trxB"
                     /locus_tag="PKW26_00095"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOQ91997.1"
                     /translation="MKKVDWDFIIIGAGTAGLAAAQYAARSFMRTLVIESDLCGGQAL
                     FIYQLENYPGLWPAVSGGTFIENMKNQATTFGATIEKMQVSSIDKKGDVFYVKSENKT
                     ITSHALLITTGATHRKLGVPGEDAFYSKGVSYCATCDGPFFRNKKIIVIGGGDSACDE
                     ANFLSNLSKDVTLIHRRDELRAQKAVADRVLQNPNIKVLFNTIVTEIKGEQVVQEIII
                     QDVLTKEKSSLAADGVFIFVGMDPQTQLINSLPKDEGGHIITDEYMQTAIKGMYCAGD
                     IRSKPFRQLITAASDGAIAAHCAGKYIRDELGEMCC"
     gene            19810..21558
                     /locus_tag="PKW26_00100"
     CDS             19810..21558
                     /locus_tag="PKW26_00100"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HOQ91998.1"
                     /translation="MSLLKKSLLSLLLLSSFFIIASTFSIKQETQIITEEITFWSEIE
                     HEKLAELLVIRMTDEELLAQIFMFGWAGSEPSPLLLSWIRERALGSVKVFGWNTDNIE
                     SVARAVTQTQELSQKNRLKIPLFVATDQEGGWIRHIKGDTSDTPGNLAIGAGGLPIDA
                     YLSGYYISRELRALGINMNFAPTIDLYTNKDSSVIGPRSFGEDAQHSAILGQAFCQGS
                     IDAGIIPTAKHYPGHGDTSDDSHGYLPKINISKEVLLDRELVPFKHLISNKVPAIMSG
                     HLSFPEITKSNEPASLSPYFLNSLLREELGYTGLIITDDMMMNGATSYAGSLSRAFQL
                     AIEAGNDIIISSTCPQLNEALWTHNLKRMKENATFNAIVKKAAKKVVYEKLVYFKSDN
                     AVPLYPDIEKIKQNIPDPDGQAFFLDQACRSITLRKGDSIPTINSSQDKIVLASSYAS
                     FFNLGLKRYPQAKRYQFSQNMGPNETAYTIEKLGDLAKDADSILLTVANERDAEVARA
                     LAKLFSEKNKASVEAQKKLIVISLLSPTPALYLENANVIFAYSYSPYSFEAVFSTLEG
                     QFSPKGILPFNDYMQP"
     gene            21555..>22017
                     /locus_tag="PKW26_00105"
     CDS             21555..>22017
                     /locus_tag="PKW26_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ91999.1"
                     /translation="MKKNIEPKLKIELSALSKQNLGEVWNFLKKHEKTSIALFSHLIE
                     NNKACLPKEQFRYFLVYQNNEKTKKLLAVFMISKSGLIFHQFADNLKILEDADIAPFE
                     DILKNKNIYSIIGKKEGTEFFQNLILKIQAIAPRTEYFYHYLRYNKNEKKTE"
     assembly_gap    22018..22117
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22118..22321
                     /locus_tag="PKW26_00110"
     CDS             <22118..22321
                     /locus_tag="PKW26_00110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ92000.1"
                     /translation="QIGGVCTKTDERNRGYAQFLVQSLVNYFQKKEKKQVALFVKENN
                     ESAKKAYYNLGFVFDSYFKIVYY"
     gene            22327..23187
                     /locus_tag="PKW26_00115"
     CDS             22327..23187
                     /locus_tag="PKW26_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ92001.1"
                     /translation="MKYIEFLDLSFSYPLEGEAKNEDQNLDASLNEKEDIKNIFEHFT
                     AELPPSFVSLIGPNGSGKTTLMLLASGRLLPTSGTARLFGIDTKTLTNEEERNLLASF
                     IYQNMELETDETVGDLLQSIFENGQHPKENLFLVQEAIDALSLEASLEKKLTALSKGE
                     MQRSLIAFSILYGSRSIFMDEPLFALEYTQKEEALSYIQSYSRRFQIPVYIAMHELEL
                     SRKWADNVLLIFPNRDMSFGSPEEVLTAEDLEKAYGIPAAMLKESERLTQRTLLELQS
                     EIEKNKDIRA"
     gene            23194..23991
                     /locus_tag="PKW26_00120"
     CDS             23194..23991
                     /locus_tag="PKW26_00120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOQ92002.1"
                     /translation="MNIVLFSDEEILEPLSLFDERAQHILKVLKKQEGDYFEAGIIHG
                     MAGKAHIQCIDERGIHFHFTPSSNGKPLYPVSVIIGFPRPIQLKRAFRDMAGLGVSDI
                     FLVGTELGEKSYLSSRIVKEGAAYEALKDGCAQAKSTHIPNLSVHDDSESCLDYLANQ
                     NKHYQNPSSIKIILDPNKGKGSLQSLFAQNYGAVDAVLDYYYMGQTDTAPHIFLSIGS
                     ERGWTENELNIFLKHSFAPCLLGERILRTETAATTAVSIILSQLAML"
     gene            23988..24794
                     /locus_tag="PKW26_00125"
     CDS             23988..24794
                     /locus_tag="PKW26_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92003.1"
                     /translation="MNNEKIKQILLSIQESSIEFAVTMSGKASKKVNGLYNPETKEII
                     LHNKNFQNDNQLIYTAIHEYTHHILNEELLERTQGLGKMKSARSHTNEFWARFHSLLE
                     VAEKKGFYTIAIETSPELQALTETIKKDYLEKNALLMSEFGKLLLEAHALCLKENIRY
                     EDYIDRVLQIPRTEAKNIVKISSASFKPEIGFENMKMLAKIGNSEKREAAESQLLQGK
                     SPDSVKSFVRARPEALDIKEELIKERGRIEKTIDQLSNRLDQINANLSVL"
     gene            complement(24795..27626)
                     /locus_tag="PKW26_00130"
     CDS             complement(24795..27626)
                     /locus_tag="PKW26_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOQ92004.1"
                     /translation="MKIKRPFAFVVLLFSFSFVLFAKSVRVGFFEYKPFHIIEDGNLS
                     GFGYEYLRELQKFTDWKFEYITRVPVLDTQGQPTSRTEKLSYSRALEMLANGELDIVG
                     SIRKTKEREELYFFPKFSSGHGYETITTLVDNDEFENRKTIKLGLRLGAVQEKDFSDL
                     FRDLFPQPIVFMYYEHYSDLIKALHETKEIDYILSTSLREFSGEQMLRKFNSFEHYFA
                     ISKTRPDILAEFNYAHDQLFLQKPSFEDRLYARYYQNQMDAVLSLTEEEKKYIKENPV
                     IQVAHETNWMPIDYEDKDGKAKGITPSIFDYIAAKTGIVFSYISSDKYKNFFPILADK
                     KNIILASFAHDYAWADKHKMIMTNPYLNLPVSAVVREHHEDIHKDSLTFVGIDGYYLT
                     EKYVLPHKNYIIVGSVEECVNAIREKKADIAFIPTYSAEKLLQKPQNARLFYYSLADM
                     PYKISFALDENCDPLLFSIINKAVNSLTEIEINNAIYNNTLYLKEPDTIQTFLYKHFF
                     LIMILVFTVLIFIVFLAFLASINFRRRRANEALSKANHELQKAFSYAEQANRAKTDFL
                     SRISHEIRTPIHIITGMTDLSFDKVSDQDFIFDALKKISSASDYLLALVNDVLDMSRI
                     ERGKTVINNDSFFISSVMDTIASLFEGKTKDKGIQLLIRTNKIENDYVYGDREHIEQV
                     LINLLDNALKYTEKGKIEVTVFEEKRASVFSLFTFVVKDTGQGIESERLRSIWEPFEK
                     GKTKIKTKGSFGLGLSIVKSLVHLMGGTVQVESTPNEGSAFLFSVPLKILQATKEKRR
                     KSDSIGEIEEMYNKEKILVVEDNDLNADIVRMMLERKNLTVDIVHDGKEAVDRLLETE
                     ATYYSLVLMDIRMPVMDGREATKLIRSSTREDLKKIPIVAMSADAFAEEIKLAQSIGM
                     NEYLTKPVRSESLYAMVYLFLRGNKKDCL"
     gene            complement(27668..28744)
                     /locus_tag="PKW26_00135"
     CDS             complement(27668..28744)
                     /locus_tag="PKW26_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ92005.1"
                     /translation="MNRTILIADDDEISRSILSEIFKKEYSLLIASDGQEAIELLEKH
                     ANSIHIVLLDIIMPRKTGIDVVKEMARTGDLHTIPVVLITGSTSKENEKISYDLGVSD
                     IITKPFDPYIVRRRVENVLSLYLHKNELEAMIAIQTDKIHEQNVHLRKINESVIDVLS
                     TVVEFRDLESGYHIQRIRTFVSLLLDKAIEKGYIVLDDMQSKIKIVNASAMHDIGKIG
                     IPDTILLKPGKLTPEEFDLMKNHTLIGCSILSKLAYVNDPEFYGYCYDICRSHHERYD
                     GNGYPDGLKGEEIPFVAQIVSLADVYDALVSDRVYKKAYSHEKSVQMIYDGECGSFSD
                     KIFDCFREVEADFRECVLKTVEEV"
     gene            complement(28878..30119)
                     /locus_tag="PKW26_00140"
     CDS             complement(28878..30119)
                     /locus_tag="PKW26_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOQ92006.1"
                     /translation="MSELIKTKTFEVDPVKTKRLKAVIDALTAGENPEAVKKEFHDII
                     KTTDAAEVAAMEQSLIEDGLPVDELQKLCEVHADLFRAGLEKGSSIKQISGHPIHTYL
                     AENKIARRKVKTLNRTRLFGSLDALKTAVQELKPIITHYTRKENQLFPYLEKKNFTGP
                     SRVMWGKHDEVREQFKLFDKASEENNSAQAKKIARVLAGKIKALFFMEEKILFPNAKR
                     LLSEADWAEIRKGEDAIGFAWIRPGAEYDPILVKPEKTSMYANFDKAVSSLTEKTEVQ
                     NPASLINLDVGKLPLEVLNLILTSLPVDFSFVDADDKVLYYSDNPHRVFPRSPGVIGR
                     DVRNCHPHKSVETVEKILDSFKKKEKTDASFWLELNGKFIYITYKPIYNKAGEYLGTL
                     EMSMDATEIRSLEGRRTLLDW"
     gene            30223..31242
                     /gene="pdxA"
                     /locus_tag="PKW26_00145"
     CDS             30223..31242
                     /gene="pdxA"
                     /locus_tag="PKW26_00145"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOQ92007.1"
                     /translation="MDTNTKQRKTIAISMGDPAGIGPEIIVKALFLLLEERRAKSETL
                     AQCAIHMKTSSSKFLLVGDRSVLEEACEITDIPKKILSDPDLEIIDLNNAAAKSFTHG
                     AVSALCGKAAYEYIEKATSLCMEGKAHALVTAPINKESLRAGSVPFIGHTEIIAELTN
                     TNDPLTLFEVKNLRVFFLSRHVSLRKACDLVKKERIVDYVVRCTKALKQLGIEEGTMA
                     IAGLNPHSGENGLFGDEEVSEIIPAIKTLQEMGYKVAGPIGADSVFHQALEGKYNSVL
                     SLYHDQGHIATKTYDFERTISITCGMPILRSSVDHGTAFDIAGKGIASPISMIEAIKV
                     AEKYS"
     gene            complement(31287..31868)
                     /gene="pth"
                     /locus_tag="PKW26_00150"
     CDS             complement(31287..31868)
                     /gene="pth"
                     /locus_tag="PKW26_00150"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOQ92008.1"
                     /translation="MISAVAFLGNHGKKYQQNRHNIAWLFARNLSFLSDIYFQTKFNG
                     QMGSIQHFADREEPLYFLFPETYMNLSGQSIGPLLSFYKLKPENLLVVHDELELPLGT
                     VSLKWAGGLGGHNGLRSTKECLGTADFWRLRLGIGRPVHGDVSNWVLSDFTDDESIIL
                     GQVFAQMDDFFLKLLTKKPENLAKKWQKQNLLV"
     gene            32000..>32781
                     /locus_tag="PKW26_00155"
     CDS             32000..>32781
                     /locus_tag="PKW26_00155"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOQ92009.1"
                     /translation="MNKNTNDKTKENAKIPQYPEKITLSCTLAKEMHPFMKTLSDGIS
                     EFSFPSLFLHRKKYNYSLSKVSDELYLLFGEQNKKSFFSVLGNFIDANQAEAESLFIK
                     LLENGLYWKNMSESQKNALSPDFLSRIEEKYTLIDDRDNADYIYLREDLAQLVGKTYH
                     KKRNLINAFEQSYTPRVEPLSEDNLADAFHILEEWKNNTSETFTDYEQCVEALNSLKL
                     DNIKSCMAFEGIIVYADDKPAAWTLGEKIAQDRSFVVHFEKA"
     assembly_gap    32782..32881
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32882..34162
                     /locus_tag="PKW26_00160"
     CDS             <32882..34162
                     /locus_tag="PKW26_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOQ92010.1"
                     /translation="NQKVSFAEAILKPVPKDGGMYVPASEDNLRPWILHLDASSSFSS
                     IAGSLTSALLKDEFSPLISENIAQTAFPFSPELKQLDKNLFVLELFHGPTGSHKDFGV
                     SYLASCLEHILHMEDKKATVLAVTNGETGACIANAFRGKKHLRAVLLYAKGTVRGFQE
                     TDVLSQGGNIIPLEINGSEEDCFQLVRDMYADQDLIEKYNLSLANSLNIGRLLPHSFF
                     YTYAFSRLKKIVHSDIFYAMTPGNYGNLVAGLYSWKFSLPVNGFIATSTPELYDDIQG
                     KCAVSNSHIPLEKRNVSDPVNPSNIERLEEIFAHNPQVIRAMVYPAKISESETEVAFK
                     ELYKNYKALLDPSTVSAYAAVKKRQDIIYADDGVAVLLSRYHPSFFADQARIWGAEKV
                     KMPEQLVEMQKKQRAEHCIEANKQALEQILKEST"
     gene            34178..35809
                     /locus_tag="PKW26_00165"
     CDS             34178..35809
                     /locus_tag="PKW26_00165"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOQ92011.1"
                     /translation="MPAQNPAILEIHRLVNKISFYTEMIYSDALLLDLHEFSEYWKDA
                     LSQISEIKRAINHKFSSKTEDSLIDTAIKEELSRRIFLFSRIINSIPADFSLHNFPEI
                     ETDIDTVQALVETAQDYFEKILFIRSEHLHNMPIEALKSASDYSLTKYLTGMVDRVKN
                     ASAGTIVFAGKLGNETAEIQDLLATASFHTINILEQESIYDIISTYDVSLICYDCGQN
                     TMEGFSVLQELIDDPVSADIPVLICGSEYSDLLAMQFIEAGAIDYYSTSRSKKTLFAR
                     IQAAIKRTQNNYHRQLYIRALELNKQSTSKEFTAASQYIADLLPRKTENDYFSINWSF
                     LPSLQLGGDIFGYYWLDDEHFMLYLLDVSGHGLEASLYSVTIMNLLNNELLGTANFLD
                     PSSILRELNRIFPIEKQNNMFFTIWLGVYNIHSRKLVYSNAGSQPAILMIDNKPHRLA
                     TKATIIGIDEDSEYENAEIQVPKNSDLYIFSDGIYEIKKINKEMMHLEEFIAFLVEAQ
                     ACKDKNCQSFIRSIEKQSITGGFEDDVSLLHLHFN"
     gene            complement(35864..39607)
                     /locus_tag="PKW26_00170"
     CDS             complement(35864..39607)
                     /locus_tag="PKW26_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOQ92012.1"
                     /translation="MKQNTIDTRIYETLANNPLMEFHISREIREKIAFDELLFASSGN
                     IIFAHFKEVREFTKKVNELFNPLIESQKMLKASHVNAMALIDEIFHYICYLFRREKNE
                     NAFAELYLHLEKSLGEKKLNDLLLAFVEEFPPLPVYKGKQSAEEYLSGSTSAKDILLP
                     GEKKQKTETEIDNKITVLEEFILLHLANENPAFEPFLFLFSNKKLVSLASYNKAWEEI
                     KDFFEEQPFIGPNNTSLIKLFKEPVEFSPLSLKGQLDYIRTHWADLLGEWLMRLLAGI
                     DFLQEEDKPGWSGHFSGPPPMEAYDYNSLLHEYERFSQDKDWMPRVVLMAKTVLVWLD
                     QLTKKYERPITTLDQIPDEELDMLAQQGFTGLWLIGIWERSWASKRIKQINGNPEAAA
                     SAYSLHDYDIAEGLGGWEALGNLRTRLWYRGIRLASDMVPNHTGMDSKWVVEKPHLFL
                     QTKDCPFPTYSFNGENLSLDPRISVYLEDNYYNKTDCAVVYKRVDNASGATSYVYHGN
                     DGTGLPWNDTAQVDFLNPEAREEVIQKILHVARNFPIIRFDAAMVLAKKHIRRLWYPE
                     PGHGGDIASRAEHALSHADFDARIPNEFWREVVDRCAQEVPDTLLLAEAFWMMEGYFV
                     RTLGMHRVYNSAFMNMLKQEENFKYRATVKNTLEFDPQVLKRFVNFMNNPDEETAVAQ
                     FGKDDKYFGVCTLMVTMPGLPMFGHGQIEGYSEKYGMEYTKAYIDEKPDLYLMERHER
                     EIFPLVKKRYLFAEVENFRFYDVWNGEHVNEEIYAYSNAYKNERSLVFYNNKYERASG
                     WIKESCQYAEKNGEEVILKTESLASALGLSFSAQAFCIFQELKTGLWYIRSSKEMHEK
                     GLFVSLNGFASQVLLNICEVKDDETGKWALLHHHLDGKGVPDIQVALTELFLRDLYKS
                     LEVFASKTFFDEMNELFEPGKVDKKKLKLILDTAKDAGLQYYDTLISFIEGNYGFSSV
                     IDKKETGLPLDAKEVWAVFEKRIQNLMSFVESAQKKTVKEAHLQALSQFIYDGILAHP
                     YMGELLVSYAIVYSVRDLIGEKATAWHSKELINLWCLDRKIRDLLVSYGRPSKDMYQI
                     FKTFIEVTRFSDYPIIDEDLSLSAYLFVEAVLETKEASLILGANSYADVLWLQKEKAE
                     FSFWAAIVLYALFTAKPKDYERLLSLYAISVKALSDASYKADTFKQLLEPKDKKTAEK
                     LLSKYYDKEVLAVLLGTKMDSAVTESKEEKAKPSKKQTKDVKK"
     gene            complement(39608..>41083)
                     /locus_tag="PKW26_00175"
     CDS             complement(39608..>41083)
                     /locus_tag="PKW26_00175"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOQ92013.1"
                     /translation="LTKAEIAELWDVVLSKEDLYFHVWIQKNASEQLQEASKYCKAKN
                     ILLKGDIPILMNEDSADIWAYPEFFQEDLRAGSPPDGENPFGQNWGFPIYNWEELKKQ
                     DYSWWKERLIEAAKYYQMYRIDHILGFFRIWAVPKGESKAILGWTIPNASMSTEELLK
                     CGLSKETIRWLSKPHVATREIEEVNNGDYLGTHGILTTCMDRIGSEELWLFKESIKTD
                     ADIWSLDIPHKIKEKLAEKWLDRALIEIKKDTYLPLWTYENTSSWKTLNDEQISMLRE
                     LFTKKEQKQNMLWEKQARELLGALSGVGNMIACAEDLGVNLQSMPRVLESLSINALRV
                     VRWNRLWEKEDKPFVPFTDYPALSVSTSSVHDSSTLRGWWDKEPDSKDFGRSFFENGK
                     QSLDYIHELFSPLVCQKVLSIMATTKSNYFINPIQDFLYLDALYYAEKSEDERINIPG
                     TVSEFNWTYKLPVLIEDLAKNKDLIQKIKEIVSIHKKSSEV"
     assembly_gap    41084..41183
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(41184..41787)
                     /locus_tag="PKW26_00180"
                     /pseudo
     CDS             complement(41184..41787)
                     /locus_tag="PKW26_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
     gene            complement(42258..43538)
                     /locus_tag="PKW26_00185"
     CDS             complement(42258..43538)
                     /locus_tag="PKW26_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ92014.1"
                     /translation="MKLSKRIILVSLFAVLAIGLGFIGCSKTTKSSQTVLKILAYGDN
                     SNAEGQNFVRIVTAFEKANPDIKVDFELLYDDAYHSKAKARIASGDIPHVAYFSSDAR
                     WGGSWREAGQLVDHTPYLNKAHYNIDAFPLQDGKREYVPLGTTNDCTVLFANKALLDE
                     LGLAMPKTYADMVAMVPVAKAKGIQVLAVGGAADWVWGSCVMSAIISRTTGLLDAPEQ
                     ILAGKKKVTDADVVAALDFLTTMVKDGVITSNAVLVDTQGAVSDFSTKKALFLLGGQW
                     EAGGIVPEIAKDTKLMSFPTIPGEKASAALSSAGAISVGYGFTKKAVEDGVVEAAVKF
                     LDYFNNDAEVIQRLRDGAIVAPVTKAAIPNDMPAIINEKVAYGAAGYPSAQVIDSYIQ
                     GDPNDLLNAGMQQIVAGKATAAEIAAKVQAALNK"
     gene            43814..44728
                     /gene="era"
                     /locus_tag="PKW26_00190"
     CDS             43814..44728
                     /gene="era"
                     /locus_tag="PKW26_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOQ92015.1"
                     /translation="MNDAKKKSGIVTIIGRPSVGKSTFLNTLSGEKISIVSPIPQTTR
                     NAIRGIINTSLGQLVFIDTPGYHASERKINQSFKALSKEQRDSSDAIVYMLDASREPG
                     EEEVLTSQLIAPYIEKTIIVINKIDLAKLDEKKNCLAENKAYIKKTFPQFDEKNIIEV
                     SAQDDTNLNEVLKALYEIVPEGEALYPEEFYTDQEVDFRIAEIVREKAIEMFKQELPH
                     AIFVSISDMEWKKPGKELWVRAFLNVEKESQKGMVIGKGATMIKTIRVESIKALRKIF
                     SYRIDLDLQVRVNKNWRQKDHILKKIIY"
     gene            complement(44800..45531)
                     /gene="rpiA"
                     /locus_tag="PKW26_00195"
     CDS             complement(44800..45531)
                     /gene="rpiA"
                     /locus_tag="PKW26_00195"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HOQ92016.1"
                     /translation="MNKSLSQSEQKQRVAEYAIDRLIEKGLLFSGMKLGLGTGSTVMP
                     AITYLAKKISSGILSDIKVVPTSFQTQIACENLGFSVFSLNAKQIDASLDLAIDGADE
                     IDIENKLVKGGGAALFLEKLIAYASKHFVIIADETKTVESLARAFPLPVEILPESRLI
                     VERGLKALGAKVAIREGLRKAGPVVTDNGNFILDALWDKESLFSASDYETKINEITGV
                     VDNGFFTKKKALVFIAKADGSVETR"
     gene            complement(45524..47035)
                     /locus_tag="PKW26_00200"
     CDS             complement(45524..47035)
                     /locus_tag="PKW26_00200"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOQ92017.1"
                     /translation="MSGSKICLCLSCSTIKENLLVIEKYRNWIDMVELRVDYLTKDER
                     LYIRRFPELAGLPCILTIRRRIDGGKFTEGEAARTTLFARGLAFADQDASKNFAYIDL
                     EEDFHVPCLQDAALAFGTKIIRSYHNMHHTIDNLAEKMETLRMTGYEIPKVAVMAHSL
                     SDVTNLYRQVQEVKKQNKGDHIVLAMGPYGLASRVLAQRFGSLLSYTSPQETLFNYYD
                     IGHIDPITLQEVYNYKTIDDATALFGITGYPLTTTGSPKIHNQGYKNHGINASYLPLK
                     AEKIEEAIEFADVLGISGLSVTIPHKEKVIDHLQSMTEVTGEIGACNTIVKKDGEWKG
                     YNTDVYGFSKSLLEFVGTKNLSRMKVAILGAGGAARAAAHVVHNLRGKACIFNRTIFK
                     ARSLAEYYNFRWASLDYESLDILEHYSDLIIQTTSVGLGVSQEEMQKEEHDPISFYTF
                     VGRELVYDMVYSPEKTPLLLRAEKAGCRIENGKKMLEYQAEAQFSLFTGEKYE"
     gene            complement(47090..48109)
                     /locus_tag="PKW26_00205"
     CDS             complement(47090..48109)
                     /locus_tag="PKW26_00205"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOQ92018.1"
                     /translation="MKIHRLREDFADFLESLEANGKALILMSLLTIMVMAVITMAVFF
                     LSVKGKEEVMVPNVVGKELSAALLEMQVKELYPKLQMKYSNFVDDKGTVLEQSPPAGS
                     IVKAGRRINLVVSRGAVIDTVKDYVGQKIDSVKLDLQSLFTGYTRPLIVLDENFSYEF
                     NEQEEGTILWQDPAPDTKISDPIVLKVGLSKGSQYDKVKIPSLENLSLNDTLLVMNRT
                     KLLYDFSFRTPELDEGIGRVVSQLPVGDTYVNAYSRVSVVFAFPEDMEENGLIYGVFK
                     ETLQEFPYAVELKLEAILPEGDRYALVQFYHLGGNVSIPYAVARGTELVLSISNKEAA
                     RFLVD"
     gene            complement(48106..49080)
                     /gene="fmt"
                     /locus_tag="PKW26_00210"
     CDS             complement(48106..49080)
                     /gene="fmt"
                     /locus_tag="PKW26_00210"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOQ92019.1"
                     /translation="MKILYAGSPDISIIPLLQILKEANHEVVGLLTNPPSAQGRSKKL
                     ILSPLAQFVLDHAEYAHIPVLAPERLDEACRQAVLALKPDLLVCFAYGKIFGPKFLSL
                     FPKGGINIHPSLLPKYRGCAPVPEAILNRDSQTGVSIQEIALEMDCGDILAQETIDLD
                     GTETSESLLADAAHISAKLISPVLDAIENDSIKKQKQKASSACYSSLFCKADGLIDWN
                     CSALDIDAKIRAFTPWPGSFTYVEGKMLKILKASVFSDSNTAEGETKKAGSVLARDKT
                     GILIQTGEGILAVEQLQWHTKKAMYWKDFLNGTKDFIGAVCSTIEGKV"
     gene            complement(49077..49631)
                     /gene="def"
                     /locus_tag="PKW26_00215"
     CDS             complement(49077..49631)
                     /gene="def"
                     /locus_tag="PKW26_00215"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOQ92020.1"
                     /translation="MKLRILGDEVLRQRAEALVDINDETRQLVKAMFVTMDEAKGVGL
                     AAPQIGLSQRLFVLRSDDDKDRVFINPHIIATSQETCSFEEGCLSVPQIYAEVERPEK
                     ITVQALDEHGKPFTLEAEGYLARIIQHEYDHLEGVLFIDRLDPELKEEAEKTIARREE
                     KKAEREKEKARKKAKLEAAKESNR"
     gene            49719..50288
                     /locus_tag="PKW26_00220"
     CDS             49719..50288
                     /locus_tag="PKW26_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92021.1"
                     /translation="MQVLQNNNPELISQFIDTHIVEALDSFIKDESIDAITKAAITIA
                     PCFGVEKEFCTLIEGETEVRANAEKKLTTSFYNNLLLLVQKTWVEASDEALKNQALEK
                     LKDCCGKDHHIDYKASYEAFIDMLHDVVYLMFGPQTKKADFSEYALRIDPGFGVFWWY
                     LQALPKKTDWSLDKTRLALLLGMFFLANY"
     gene            50317..51573
                     /locus_tag="PKW26_00225"
     CDS             50317..51573
                     /locus_tag="PKW26_00225"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOQ92022.1"
                     /translation="MNFEQVCQQLRVGANKLSQVNAGEKNAALQAVAIALDKNRSKIL
                     EANKKDIETARASGMKESLIDRLMLDDKRISSIIESIAIIINQTDPIGVVETGWTSPE
                     GLKIRKVRVPIGIAAIIYESRPNVTVDAFAIAYKSGNAIYLRGSSSALKSNKAIVAAI
                     KEGLAEAKSGVLEAIALAETGERSEVDEILNAVGKIDVVLPRGGAALIKLVVEKARIP
                     VIETGSGICHLFVDKSADLEQAVEIACNAKLQRPGVCNAIEVLLVHEAIKENFLEKLG
                     KTMKGKLLFKACPESYAVLNRIEGLQVEKAGDTDFDTEFLDKILAVKVVSTLDEAIDH
                     INTHNTKHSDSILTKNISHAQKFQNQVDSSCVYVNASTRFTDGGVFGFGAELGISTQK
                     FHVRGPMGLSALTTEKYLIDGEGHIR"
     gene            51573..52412
                     /gene="proB"
                     /locus_tag="PKW26_00230"
     CDS             51573..52412
                     /gene="proB"
                     /locus_tag="PKW26_00230"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOQ92023.1"
                     /translation="MSETIDTYNAGKNSCPTIQEILKTARKIVIKIGSNTLATSDGKI
                     NIPFMESFALQCASLMKKGKQIVIVSSGAQVAGLSTIDRWERKKDIHYRQALCAIGQV
                     ELMDNWRNAFWKNNIHIGQLLLTKDDFHDDNRTLNMRNTLFTLVDEGVIPIINENDSV
                     STEEIRIGDNDNLSALTAILWNADLLVLFSDIDGVYTDNPKTNPEAKLIEYVPSMDDL
                     RANIRLGSANSFGTGGMNTKLEAADKLTSYGIPMILANGSHEKILEKLADGSQKATLF
                     CAE"
     gene            52511..53353
                     /gene="proC"
                     /locus_tag="PKW26_00235"
     CDS             52511..53353
                     /gene="proC"
                     /locus_tag="PKW26_00235"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOQ92024.1"
                     /translation="MNKIGILGCGVMGEALAKAMLKKTEGKNIFLFDIDTERTKKLAE
                     KLSCKTAPSSSDLVKTVDFLLLAIKPIHVKNLLTEIASEYYQKKPVLISIVAGIQIES
                     LENMLYSTLPLKTEEKKVKTAFPIIRLMPNVSVLVEEGMIAFSSNDAVQAKEKDFVID
                     LLEKTGKIEIVPENLMDAVTAISGSGPAYGFLFIEALADAAVLLGIPRKQAYIYAAQT
                     LKGAAEMVLETGEQPGVLKDSVCSPGGTTIEAVKKLEEKGFRSAIMEAAEAAFKKSCS
                     LGKK"
     gene            53416..57351
                     /locus_tag="PKW26_00240"
     CDS             53416..57351
                     /locus_tag="PKW26_00240"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOQ92025.1"
                     /translation="MYRIYVEKKTGFQVEAQKVEQELKDFLGINGLKSCRFLIRYDIE
                     NLPLALSSKAIDEVFVTEQTDRVFINHIPLAEDAIVIAWEFHHGQYDQRALSAQEALN
                     LLLVKHKATAQDDTKKAIIVKPAKMLVLQGDINASDIKKIESYFINEVDSQKANLDIP
                     KTLSKAIPEPKTIPFYQDFIKLDEKGLLAFREEQGFAMDNADLVFVQDYFKKEGRDPS
                     ETEMRVLDTYWSDHCRHTTFNTILDDIEIEEGYFTDLFKKSLESYTTLRTEIHTTKEK
                     PITLMDMATIGAKYLKKEGKLDDLEESEEINACSVFIDVHTENAETKEKQTEKWLLMF
                     KNETHNHPTEIEPFGGANTCVGGGIRDPLSGRSWVYQGIRITGSANPRRKLEKTREGK
                     LPQRKITKDATAGFSTYSNQIGLNASQAVEIYHPGYEAKRMELGALIAAAPAEQVKRG
                     IPQAGDLVVLLGAKTGRDGIGGATSSSRVQTAESVETASAEVQKGDPLEERKILRLFR
                     NSKVSSIIKRCNDFGAGGVSVAVGELAPGLHIDLDAVPTKYEGLNGTELAISESQERM
                     AVVIDAKDLDFLIDEADKENLKGTVIAKVTDTNRLVMQWKEQTIVDLDRNFLDTAGAK
                     RHAKAKIAEPNRIYSPLARPLDAVQLVLNDESRGRCKKETLKDAWFTNMSSLACCSQQ
                     GMAEQFDASIGANSLLFPMGGKTQSTPEVASVAKIPVLSPKETSTTSIMAYGFDPLVA
                     QWSPWHGAQVAVLESLAKIVSVGGNLETTRLSFQEYFERTDSPETWGKPTAALLGALD
                     AQKAMEIPSIGGKDSMSGNFDDLKVPPALISFAFSSSDEENLISGAFKQGDSYVYAAI
                     QAYTEMLAPDFDAFKENYAHIKSALKEENILSMYPVGTGGISEAISKMSFGNEIGCTI
                     ESGVQELLFVPMYGTIIVETKEKIDTSKVRWLYIGRTQEKPVLSLGLHCYDGGKKNKT
                     KETELQNIEISIKELKEAWKNPLSAVFPLESTEKIQKIPGFAKKRHSSLKKHDKALKN
                     KNFYHIKEKKPLVLLPVFPGTNGEYDMERAFTLAGAKTKTLIFRNKTEESTIESFEAL
                     KKAILEAEILAFSGGFSLGGEPDGLGKYTASILQYPLIKDAIMQFLSKDRLILGVGDG
                     FHALLRSGLIPFGKIKENTTGPILSANRIGKHISRMLDTKIISAISPWAPKECLKDSI
                     YRLPFSSTDGRLVLSREVAKQLFKNGQVFSQYVDADGITAKTETKNPSASFYAIEGLT
                     DPSGKILGKMAHNERSISFKEIKNASELFKNIPGNKSQDIFSAGVSFFN"
     gene            57482..58483
                     /locus_tag="PKW26_00245"
     CDS             57482..58483
                     /locus_tag="PKW26_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92026.1"
                     /translation="MKSITFKSISKLILGLLVILVLIACSSTPTEAHWLYDFEEAQGR
                     AEQESKNILLFFVGSDWDEVSIALSNDIFPSKSFLSKIASEYILVQLDFPQDENLITE
                     EQFYRNYDVANRFSLEGIPSAVLTTKEGYIIDKYNGIADDEISDAEKAAEFIKQIQKS
                     KKKSTTIISTVKKMDKTEGPKKAKLVDTILNQLNPDYTYLHTELIAQIPEHDPDNTTK
                     LHEKYLLQLTYPKAMEALYTGDYENAIQMLVEVAENKKISKKDSQEAYYTAAYLNEQM
                     NLGKEQTLIYLQLAHDVDPESEIAQNVQQIISMIQLEMEEEEAFYYSEEYRTEGNAN"
     gene            58473..59750
                     /locus_tag="PKW26_00250"
     CDS             58473..59750
                     /locus_tag="PKW26_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOQ92027.1"
                     /translation="MQTNSLSSLWFIGLLFFSGVLIFLSMVFSASESAFLSINTLRLR
                     LLKDKKHKQAMRSSKLLEKKELLLNTILIANNIVNISLSTVITAICLELFGNAAISIA
                     TGIVTLVLLVFGEISPKTIGTHHPETIAFLFSGFIQFFSLILKPFVIFFSFFSNLFLK
                     LLGIKKKDKTKSFSEEEIIHFIEIGEEEGVVEAIEKKMLHRVFKFTDLDAKNIMVPRM
                     QITFVKSDASYTSIMELSQKTHLSKFPVIQDTIDTVLGILHVKDMLPFICDKKSFAVK
                     KIMREPLYILETTRMSQIQELFRQHNQSIAIVLDEYSGTSGLVTKEDFTQEVFGTMYD
                     EYDSSEIPEIQKLSQEHFLVSGNSRLFDINKKTGLNLSSDYYETLAGFIHEQLDDLPE
                     EGQELFFEDYRLRIEKKEKQKILLVSLFIQVNE"
BASE COUNT        18856 a        12453 c        10465 g        17729 t
ORIGIN      
        1 gctctcgatg tttttgcaag taaaaaaaac ctacgtctca ttgtggcaga tattcccagc
       61 gacgaagatt gggaatatct ttcagttgat ggcggcttac tcgtacaaaa tagggacaga
      121 gcattattcc aacgttggga gcctgttaca aagcacgagc cgactccaaa gcaaatagaa
      181 gagatgatct ttggcatgat agtcgcaatt tttgtaaaat caaatgcaat cctcatctca
      241 aaggataaaa cagcgatagg aatagctggg ggacagacga atcgtatttg ggcggcatct
      301 caagccctcg aaagagcaaa acaattcacc gataaagaaa gactttcaaa tgccgaagtg
      361 ttaataagtg atgctttttt tccttttagc gatactgtcg aagaggctgc aaaatatgga
      421 ataaaagcaa ttattcaacc cggtggttct ttgcgcgacc aagaatccat agatgccgca
      481 aataaactcg gaattgctat gatttttact ggctgccgcc actttaaaca ctaacagcat
      541 attttcagga gaactaaatg ctttttcatc tcgctagcta tttagccact ttttttggac
      601 cagctcgatt attaaaatct tatgctgttt taatcaccct cgccttatat ctcggtttct
      661 tcataagtta tttcttccta cctaaactat acccaaagct tccgagggac agaggaagag
      721 atttcacccc tggttctgaa gcagcaaaag gaaaaccgac tggtggcggt gctgttttta
      781 taagtatttt tgttatcata gccttctttt ttgtaccgct caccaccctt caaatagctg
      841 tagttattct cacttggatc acaatgatcc ttggttttct agatgacaag agtagcctcc
      901 cttggggaga atatcgaaaa gccttgttcg atcttctcat ttgcctagta gcttctttta
      961 ttctttacca cttaaaccct aaagttttaa atggcgaggc actctatatt tggcttcctt
     1021 tttttacaca agcctttgct attcctcaat ggccttttat actacttagc acaattattc
     1081 tttgggtttc cataaataca acaaattgca ctgatggtgt cgacggctta tcaagtaccc
     1141 tcgccctcat cgcccttatc actatgggaa ttatttttta ttttattatc gggcataaag
     1201 atatttcagc atatttactg gtaccgcatc taaaagacgg tgcctcatgg gcaattctta
     1261 ccttcgcgct ttcaggtgta ctcatgggct acctctggca caatgccttt ccaagcaagg
     1321 tactcatggg agatgccgga agtcgggcgc tcggattcta cattggcact tctgttatag
     1381 tttctggaaa ccccttcctt ctccttgcaa cttcttctat tattttactc aatggaggca
     1441 tgggacttct caaagtattt cttctgcgtt ttttcaaagt acgcttattt caaaacattc
     1501 gttttccttt gcacgaccat atgaaagatt ctcgacaatg gtcatcaacg caggttctcg
     1561 taaaatttat gattttacaa atacttatta caatagcaat gttgggctta atatttaaag
     1621 tccgttaaac aacttgtagt aaaagcgttt ttttcatatt ataaataaaa tgagtcctaa
     1681 tctcagaata tccgtggagg ttttatgagt tcaattttag acatatttgg ctcaaatgtt
     1741 tttaacgatg cagcaatgca agaatatctt tcaattgatg tatacaccgc attaaaaaaa
     1801 acaattaaag aaggctctcc actcgacttg cgtttagcaa acgctatagc aaagggtatg
     1861 aaagactggg cactttcaaa aggtgcaact cactatacac attggtttca accgatgact
     1921 ggcattaccg cagaaaagca cgatagtttt ttatcgagag ataaaaacgg agatgccatt
     1981 atagattttt ctggaaagga attaataaaa ggagagccag acggttcaag ttttccatct
     2041 ggtggcttgc gggcaacctt tgaagcacgc ggctatacca cctgggaccc cacctcctat
     2101 gcttttttaa aagacaatac gctctgtatc ccaacagctt tttgctcgta tggcggcctt
     2161 tctcttgata aaaaaacacc gctcctacgc tcaatgaccg ctttaaacaa acaagcgctt
     2221 cgtattatga aactctttgg cacaaaaaca tcgagggtta tttcaacagt tggaagtgaa
     2281 caagagtact tcttaatcga tagaaaactt ttttttaaac gtaaagacct cgttttttgt
     2341 aatcgaacgc tttttggtgc acatcctcca aaaggacaag aactcgacga tcactatttt
     2401 ggtgctatta aaccgcgcat cgcctcttac atgaaagagc tcgatgaaga attgtggaaa
     2461 ctcggtattt atgcaaaaac gaaacacaat gaaaccgctc ccgcgcaaca cgagcttgcc
     2521 cccatttata ccgaaacaaa tattgccgca gatcacaacc aacttactat ggaaataatg
     2581 aaaaaaattg cggtgaaaca tggactgacc tgcttacttc atgaaaaacc ctttaaagga
     2641 gtaaatggtt ccgggaaaca caataactgg tccatatcta ccgactctgg cataaatctt
     2701 ttagacccag gagattcacc ctcagaaaat gcacagtttc ttcttatttt aaccgcgatt
     2761 attaaggcag ttgacgaaca ccaggactta ctacgtctat cagttgcaac agcaggaaac
     2821 gatcaccgtc ttggggggca cgaagcccct ccagctatta tctctatttt tctcggagaa
     2881 gaattgacaa cgattttaga atccatccgc gatgaaaaac cctacaataa aaaaaatgct
     2941 gagcttctca ccctcggtgt cgacacgctc ccctctttcc caaaagacag caccgataga
     3001 aatcgaacga gtccctttgc attttctatg aacaaatttg aatttcgatc gctcggtgca
     3061 agcgtatcta tttcttgtcc taacattatg ctcaatacaa tcattgccga cgctttttgc
     3121 caattcgctg atgttttgga aaaggcggaa gattttacat caagcctgaa ccaacttatt
     3181 cgagataact taaaaaaaca cagccgaatt attttcaatg gaaatggcta tgatgatgcc
     3241 tgggtaagag aggcaaaaaa acgcgggtta ttgaacttaa aaaacaccgc cgaagcactc
     3301 ccgtattttt tagatacaaa aaatcgcctt ttatttacaa agcacaaggt acatacagag
     3361 cttgaaatgg aatcgcgtta tgaagtactt atggaaaact attgcaaggt attgcacatt
     3421 gaagcgctta cgatgattga catggcaaaa aaagatatat tcccatcggt actcaaatac
     3481 cttcatgaaa ttgcaaacac ggtgaacgct ttaaaaactt ttttgcctca agcaaagtta
     3541 aagggacaag agcgaatcgc acggacactc gctctcttat ttgaccaagc ggatgagcaa
     3601 ctgcaaagct tagaaaaaga agtaggcaag acaaaagata tagaaggagt tacgaagacg
     3661 gcgacatatt atgcagaaaa aattatacct gcgatgaatg cattaagaga aagttgtgac
     3721 gaaatggaat gtctcacttc aaaagagcat tggccctttc ccagctacgc tgatttactc
     3781 tttcgagttt aaaaaaatga tagaaattat agatataaaa aaacactaca caacaaaggg
     3841 gcaagaagca caggcgctcc gtggcatcac aacgaggatt caaaatggtg agtttagtgc
     3901 gctctctggt ccttcaggtt ctggaaaaac aaccctgctc aatctcatag gctgcctcga
     3961 ttcggcgagt tcgggttcta tctttctcga tggaaaaaat cttgtctctc ttacaaaaca
     4021 agagacaata aaaatacgac aatcaaaaat aggctttatt tttcaaagtt ataatctcat
     4081 ccccgtctta tcggcctatg aaaatgttgc gctcgctctt gaagttcaaa atatgttttc
     4141 aaaaacagaa ataaagcatc tcgtttatga aatgctaaaa aatgttgggc tcgaaggtta
     4201 tgaaaaaaga aaaccaagcc agctttctgg aggacagcaa caaagagttt ctgtcgcaag
     4261 agccctcgta aaaaaaccgc agatagtttt agcggatgag ccaacagcaa acctcgactc
     4321 aaaaaatgct gaaactattt tacatctcat gaaagaacat aatgaaaagc aaaaaacaac
     4381 ctttcttttt tcaacccatg acgagcttgt tctctctttt gtaaacagaa aactttcctt
     4441 acgcgacgga ttgcttatcg ctgacagcaa agctcaaact aacggcgaat aaaatcaaca
     4501 aaaaagcttg cgtactcatc ttgaattgtt gtgactaagg attgtattgc tcgctctttt
     4561 gcacctgctt catgaacatg tccctctcgt ttattgtcag aatagggtaa taaaatcttt
     4621 gtcgtttttt tatctactaa atttgcagaa aaaatatagc gtacatacac atttcgctct
     4681 gatggcacgt caacaaaact cactgctgca tctaaaagat aatttgcatt tgacgttcca
     4741 atataggttt taaaaccgta ttttgcgagc attgtcgaca aggcaagccc aattctgcca
     4801 tcatcatctc caacaactga aatatctata gctatcgctt ctgcaaccgc atatgaaaat
     4861 tctttaaccg atacaatcga gccatagcga aacgtattgc tctgagctgt ttttgcatcc
     4921 atcgcatata aaatatctat atactgttcg ttttcttcgg ctaaagacag agcttgtaag
     4981 gcaaggctaa aagaagaaaa ttgaccgaga gaattttttg ctacagaaat tttctccatt
     5041 atttcgtttt cattttcacc catttttctt acatagtact ctgcggcttc ttgtttatct
     5101 acataggcaa gggcatagac tatatcgttt ttatcccacc aaaattcagc aatttttaca
     5161 ccgacaaggg catcaacaac agcataggtt tcaataattt catcaaccgt cgattgatta
     5221 gaagaggcac ctgtagaact taccgcatct ttttcagttg taaccaatgt agatgaaacg
     5281 ttttgtttta ttatacgtgc aatggaagcg atagcagcat tctcagcctc actcgtagat
     5341 ttgcctgaac caacagcgct taaatagcgc gatgatggga aacttgtttg agtgtttttc
     5401 acccaatctg gttgttttgt gcttgtattg atattttgag tgataactat ttcgggttcg
     5461 tcaaactggc ttggcttcac tgttcttcct ggcaaagatt ctggaatatc tgctatgcca
     5521 atcccctccc ccatttcgtc cataaagcct ttgtttgcag cgtctcgtgc tttttgtgca
     5581 gcttcacttt tgttttttcc ctcggtttca tctaaaccag ttaaaccaga gctagtcata
     5641 caagagaaaa aaacgaaaag ggaaaaaatc aggcttgtaa aaaaaagccc tcgacctatc
     5701 ttttgtccta tatttttatt ttgtcttttt attgtaaaca tataccctcc actagattga
     5761 tagtattttt tttcaggtca aagccttgta ttgtttcaag ccaaatagtt ccgccccaag
     5821 catctttgta ttgtatctgc actgtatatc ttcctggcgc taaatgtatg cctccaaccg
     5881 aagcctttgc agggaaataa cgtgaggttc gcaaatcggc aacctcggtt agctcgttcg
     5941 atacaaggga aacaatactc aagggcccta gtatgccact tgcatcaccg ttttgatcaa
     6001 cgcgacttgc tgcatccaaa acgttggtag tcatggactt tgcaatagaa cgcgcaatcg
     6061 ccttaaaata aagtaggtcc tgtttttgtc taaaagtatc ataggcaatt gaatcaatcg
     6121 actcgagggg gtctaagcga aattgatgtt tttctcccgt atcttcattt tcgactgcaa
     6181 cgcttatcga gacaattcga gaagctcttt taaacataat cgggagcgcg agtttataat
     6241 aggtatcact cgtataaaaa cgtatcactt cttctgtttt tatgggagca agccccgtaa
     6301 agcacaaaat attcaagcgt tctaaacctg gaagttttag aagctcgtca tctaaactag
     6361 atggcaagga aaaaggatag agctggcttt ggtcacggaa ggctgttttt atataacgta
     6421 aatcaatttc tgcagcatcc acattccctt cagccctata caaaagcata ctcaaataac
     6481 gtgcgagagc ggaattgtga aaagacattt ccccaaaata tgcatcggag agggagtttt
     6541 gatttgcctt gttttgagct tgtgcataat ttaaacgcaa gtttttttgc ttcgtatcaa
     6601 agcgacgaat ttctaccaaa gctttgtcta tttcatttaa ggcaatataa ttcaatgcca
     6661 taaaaagatt tgaatagata tcttcatagg ggtcgccagc gtaatcgata accgtatcat
     6721 ttataataaa ggattgcaca gattgcgaaa tgctttttga aaaatactca aacatttttt
     6781 cttcggcata ggccaattct ttgttcgaag tcttgtagtc gccgccatag tgcaagagca
     6841 aacctttatc aagtgatttt agaacaagat ctttttttgt atacaaatta tcttcatttt
     6901 tttcgatcat cgaaactgcc gtctgataat cacctcgtcc tacatgagaa tcaacttgat
     6961 taaaaaaatg cctatttgtt acgcaggaac taaaaaataa aagggcaaaa aatgagaaaa
     7021 aaagaaatgt tatgcgaagc tttttgccct gcatctagta ctcctaccat cgaacagcgt
     7081 ctcgccgaat atattttttt atactcgaat tttccccaag ccacaccttt gtgttttttt
     7141 ctatattaat caattctgca gtaacaaaat aggtccgagt aaaatctgca ccagagctgt
     7201 caacaattgt tttcaccgca ccgatgagca taaaatctgc tgcaacttca tttgcaagcg
     7261 cttttgcaga aagctcacta gaccattcct gctgattttc tctttcatct cgaagctctc
     7321 cacgttccat gctactagca acaaaatcta ccttgccgct attcacaagc gctatttcaa
     7381 atttttttgc caaaatactt gtgtcaagat gctcatcact ttggtttcta aaacttccga
     7441 gaataaccac tggtggtctt tgatactggc gaataaaatt gacgatactc ggtgcattca
     7501 cacattcttg taccaatgta tctgcgataa tacgtacatc ggtatcgttc caatagccac
     7561 tcaaatctgt ttgagttcgt gcatcaatgc gattaacctg cgtcccagtt gtagaaacac
     7621 aagaacttaa aacaacaata agcatcattg caattgcaat aatcgatgtt tttttcatat
     7681 ccaaacctct ctttttgtgt cctagacaca atcctttttt agttatttcc caacataatt
     7741 gacgcaagca cctttgcatc atctatcatg ttttctatac gacaatattc attcggctga
     7801 tgtgctgttt catcaagggt gctccatacg gccgcatgaa gtccttcatt gcgtaaatat
     7861 gaggcaacag ttccaccacc tataccgata agccgagcct ctttccctcg caatttttga
     7921 attgcttttt ttaatacctg tacaacttct gcatcatgag gagttgcagg cgactctacc
     7981 gcttgctttt cttctacaac tatctctacc ccatattttt ttttgatgct ctttgtacgt
     8041 ttttcaattt ctaaacgaac ttcatcaagc gagtattgag gtaagattct acaatccatg
     8101 aaaaaactat cttctccagg aattgtattg atatttggaa cgtttgcttc tttttttgtc
     8161 ggctcaaagg ttgaatagac tggctcaaaa agtgagtttt gcgctgaaaa atgatttttc
     8221 atatcgtgta aaagaagggc taaatcacag gcagcaagat gcgcatttat cccttcatcc
     8281 gggcgagagc cgtgacattg ttttcccagt gtttttactt gaaaccacaa aatatttttt
     8341 tctgcaattt ctatacaaag cccctcacta tcccctccat ctggaataat aataagatca
     8401 tctttttgaa aaagagcatg attttctaac aagtattgga taccgtatgt tgaacccact
     8461 tcttcatcag ctacaaaaag aagtttcacc gtatattcag gaatttgctt gtttgcaaga
     8521 agcgctttaa gggcaaaaat agacgaaacc aatccttgtt gattatcttc agtgccgcga
     8581 ccgtagagtt ttccgtcttt ttcgagcatt tcccaaggat cgctgtccca aagcgataaa
     8641 tctcccgtgg gaacaacatc gagatgtgtc attatccata ggttttggct ttttttacca
     8701 ggaagagtcg caataagatt tggtcgaatg ccagagtcag cccgtgcatc tggcgcatca
     8761 aagcgttcaa ggttttctat gccgttttct ctcagccatt tttctaaagc aaggcatttt
     8821 tgtaattcac ccttaccacc gctactggga tcgagggcag gaattgcagt taaaattctt
     8881 tgcaaggcta tcatttcatc cttgctttta tctattgttt catatattga ttcacttgta
     8941 ttcatatttt tttccctact ataactataa cactttaaag tgcaaaaaaa acacttttta
     9001 tcctattttt tatatacctt ccacgttgtt tcaaccaagg ttttagcatc cgaatactga
     9061 gcttcccaac caagcaagtc ttttgctttt ttggaaactg catataaaca ttcagggtcg
     9121 cctgcccttc tttcaacaaa atccgttgga atttccttac ctgacgcttt tttcgctgca
     9181 tctaaaattt ccaaaacgct caaccctacc tcgcttccga gattaacgaa aaggctttta
     9241 tcttcttttg taatgtagtc aagtgctttt acatgtgcta tcgctaaatc ccaaacatgt
     9301 acataatcac gtatgcaagt cccatctctt gtttcataat ctgttccaaa aacctgtagt
     9361 ttttcacgca taccagaggc aacttccata atcacgggaa gtaagtttgc aggattttgc
     9421 tcttttgcag taatttcacc ttcaatatca taacctgccg cattaaaata acgcaatgat
     9481 gcataacgca agccttttag cctatcgtac cattccataa ttcgctcaat ttcaagcttt
     9541 gtaaaaccat aataattttc aggatttttc ggatgatttt catcgatagg aaggtattct
     9601 ggctcgccaa aaatagcagc agatgaagaa aaaaccattc ttttgcaatt atgtgcaagt
     9661 gcagcatgta aaatattgag cgttcccgta atattattga tagaatactt ttctggaata
     9721 agcatggact cgcccgctgc tttaaaagcc gcgagatgaa caaaggcgtc aaaaccttga
     9781 gaaaaagcag catctaagtc tgcctgtatc aaaatatctc catggataaa agcattttgc
     9841 gtaaaaagat tttctctcat tccagaagaa agattatcaa aaaccacaac ctcatgtttt
     9901 tctttcatga gggcttttac tacatgactc cctatatatc cagctccgcc aataacaagc
     9961 accttcatct gtctacccct tttcttttaa gtaaagttag tatatcacaa gaaaaaaact
    10021 ttgtcattct aagctcaaat cttaaaagca gtaatactga atatttaata cgaggcatga
    10081 gtatagtatc tttttagtct cagggtaaaa tactatctga atgagaacag attttaagtg
    10141 ccttcttttt ttcttttctt tttttgtccc ttcgctattg cgccagatcg atgtaaggca
    10201 aattttgcac tcgcaggtcc taccagttca taaagcactg aagaagatag aataatagat
    10261 aaaagcatca tacccgaatc ttcaggaagc aatctttgac ccaaaaaagc aagccctatc
    10321 gccacacctg cttgtggaac aagtgcaaag ccaagataat ttctaatttt atgcgaagta
    10381 cctgttaccg cacaacctaa aaaggctccg aagtatttac ctaaaatcct tacaaaaaaa
    10441 taagtaagcc ctatcaagcc ataggtagta aaagatgtta aatctaagtt cattccagag
    10501 acgataaaaa aaagcgataa aataggtggt gaaaactttt caacttgttc atacaaaacc
    10561 ttatcttttg taagattaat atagcccatt ccaaaagcca tacatgcaag taagggagaa
    10621 acgtgcacaa tagcacaaag acctgctata cctaacaata aaccgagcgt taaaatgagt
    10681 cggttatctt cacttcgagt cggcgtcata aggcgactta aaataaaacc gcatagaaac
    10741 cccaagccaa ctgataagac attatacaaa accgttaaca agatggattg tatcgatgca
    10801 acttgcccgc cctcatgccc tacaagtgct gttgcaatgc taaatacaat gagacagact
    10861 gcatcatcga gcgccacaac ttgtaaaagt gtatttacaa actttcctcg tgccccatat
    10921 tggcgaattg ttacgatagt actcgctgga gcagtcgcgg tggcaatagc gccaagcaaa
    10981 agagaaaagg caaagctcag tcgaaaaaca aaaaacataa caatggttac aataacacca
    11041 gcaaaaagtg attcaaaaag agtaataact aatacttctg gtcccgtctt tttaagtttt
    11101 tctttcttaa aaaaacgccc cacactaaag gcaataaagg taagtgcaat atcgcttata
    11161 aaacccatgt ttgaaatcat ttgctttgga attatattga agacactcgg tccaattaaa
    11221 acccctgcta aaatataccc tgttacattt ggtaagcgca attttttagt aacacgtgtt
    11281 aacaaaaaac cagaaaaaag cataatgcaa agcgttagca atataagact tacacttgaa
    11341 agttgctcaa tccacaaaat gaaggacccc ctaaatgagt atattttaca aactaattgt
    11401 tttaaagtac atacaaatcc tgcctatcga aagacagaga gaattttgcc cgccttatct
    11461 atctctattt tttttaatgc agattttcta tagtaggctc tctcgttttg tctcaaggtc
    11521 ttcccaagca agatgcgcat cttccatttt ttttccaata acagttatct cttcttgtat
    11581 ttttttactt ttatctccat ccatatatac ttctggtttt gaaagatttt cgtgtaggac
    11641 agcaagttgc tcttcaaacg aagctatttt ttctaaaagt atttctaagt ctttttctag
    11701 ttttcgcatt tcggagcggc gtcttttgga ttcttcccaa gatagttgcc ctggattttt
    11761 ttcttctttg ctttctatct ttccctgcgc tttatcttcg cctttttttt gctgcacata
    11821 gttttcagac aaggtatttt ctgcttctat tttttctaga tagtagcgat aatctccagg
    11881 aaagacccgt tgttttcctt cttttaattc cagcaccttt gtagcaaggc tttctataaa
    11941 gcctctatcg tgcgaaacaa aaagaattgt tccaccaaaa tcactcaaag cacttaaaag
    12001 cacatccttc gaatgcaaat caaggtggtt tgtcggttca tccaaaatca gcaggtttac
    12061 aggcttgagc aataaccgca aaagtgcaag tcgacttttt tctccaccgg aaagcacatc
    12121 gagacttttg tatacgtcat ctccccgaaa taaaaaggat gcgagtaaat cccttgcctt
    12181 tggaacaagg tcgagcggac attcttcttc aaggctttcc aaaatacttt tttcaccgca
    12241 tattttttct gcattatcct gtgaaaaata cccgatgttt acacccgaac ccaatcgaaa
    12301 gcttccctca aagtttgcat cttccccact taaaatacgc aatagtgtgg tttttcctgc
    12361 accatttctt cccgcaataa cgaagcgctc tcctttttca atgacgagag aaagttcttt
    12421 tagaatccat ttttctccat cataggtttt tccaatgccc tctaaatcaa gaacaatctg
    12481 tccagaatgc ggggcaggag gaaagcggaa tcgtatctgc tttagatttt caggtatctc
    12541 tatgcgttcc attttgtcga gcattttttg acgctcttgt gcctgcgcag ccttcgttgc
    12601 ctttgctcca aaacggcgaa taaaatcttc gagttttttt atttcttctt gttgttgtgc
    12661 atagcgcgcc aaaagtcctt ctatttctac ttcacgctgt ttttcatatt ttgaataatt
    12721 tccggcatag ggctttaagc ttcctgaaaa aatttcatac acttcgttta ccgttgtatc
    12781 taaaaaataa cggtcatggc tgacgagtaa aaaacctccg ggaaaatttt gtaaaaattg
    12841 ttcgagccag tttcgtgcct ccaaatccaa ataattcgtc ggctcatcga ggagcaggat
    12901 atctggatct tctaacaaaa cctttgcgag ggcaattctc atttgccaac cgccagaaaa
    12961 ctcttcaact cttcgtgaaa aatcacttcg ctcaaaacca agacccaaaa gaatttgttc
    13021 gacaagtgca tctcgtctat accaaagcat ttgctcaagt tcctcatgaa tttcttgatg
    13081 acgatgtaaa agcacctttg tatttgtcgg tttttttaca agggcatctc cgattgcctc
    13141 tagttccgct tcaagttcat agccccgctg aaaagcagaa tcagcttcgg cagcaagact
    13201 tcttcccttg tgtacgatac cagattgcgg caaatacgaa atctgtgttt ctttttgtac
    13261 cgctctttca cctgaatcag gatgtataat gcctgcaagc accttcatga gcgttgtttt
    13321 tccagaacca tttgcaccag caagcgccgc ctttgtaccg cgagagaggt ttatagaaac
    13381 atctttcaat atatctctgt cgccaaaagc caaagaaacc ttagaaaact gaacaaagga
    13441 catcgcaacc tactttaaga aaaagaggat tataggattt tgactttgac tagaaatcag
    13501 agcattccta ataagatttt ctggcacaaa ttccatccgc aagcctgttt cttctagctt
    13561 gtataaaaag acaatttctt tttgatcgct atcaaaacgg aaactgagca caccatcata
    13621 gtaaaaactc aaatcatttg aaataaaata atccatggaa acttttccac gacttcccac
    13681 accagaggga ataaggcttg ggctgagaag gctgtaacct gtccacacaa agcttccctc
    13741 atctaaaaat tgaagcaagc cataattgga acttgaaaaa ctcggacctt gatttataat
    13801 tgtttgcagt aaaaaagaac gacgcttttt ctcggcatca attatatccc gaatcggact
    13861 cgaaacggtt gaaaaggcat gaatttcctg cttcccaaaa tctccaacaa gtgtaacttg
    13921 tatttggttt tcagagacaa ttcgtagaag caagggactc gcagttaatt gatataaatc
    13981 atcttctttc ttctccacat cttcaaaggg aaaagacttt gttatatttt ttaggcgaag
    14041 acttgcaatc tttgtttcag tattgatact aaaaccgtag tccaccttca tttcgtcaag
    14101 gttgatgcgc ccgttttgaa tcatcgtttg ataagactgc ggataccagg ttttttttgc
    14161 aaaagtctca aagcgatcgt cttttttttc ttcttcgaca tagttatcat ccgtttcatc
    14221 aaaaagactt aaattatatg aaaaacacca gcccacgctt ccatcctgca tgagcacacg
    14281 aagccagtct ccttcaagac cattcaccgg agcaccctct cctgcaaaca acacttttac
    14341 tatctcgcct tcttttaatc gatatacctg tctcccaaga tttgttgcct cataacgcac
    14401 ggggagaccg tcgagtttga ctcttgcgta gatgtttttg taggccttat attttttcgc
    14461 agtctctatt gcctgcgctt ttttttcagg agtacttatt tgccaaagcg gtacctctat
    14521 ttttttatca gaattcggca ctcctataac aaagacttga ttgatatttg attgaataaa
    14581 aacaggtaca atatcgccgt ccgaaagccc ttcttctggt agtgaccaaa gcacaacgcc
    14641 aaagcctaaa ctatgcgaac aagaaaaaag agtgaaacta agggccaaaa taaagccaaa
    14701 aaaacaagta tattttgctt tcatatcgat aagaataaca cattatccgt ataaaactca
    14761 agcgtttcag aatttccact cgtatcttca aaaaagtcat ctgaagaata gtctacttgt
    14821 ggaagcgttt agcgaaaatt ccaagcatct gattcttgta caaaggtttt atcttcgtca
    14881 acattatagg aaataccggc tgcatttgta attataagca ttatttcatt gcggtttgta
    14941 tttatatccg ggtatctatc ggtatagata tccggttttt cctctgtccc tccacgaggg
    15001 cgaccggaaa atgtttgcac tggaacaaaa tcacgacttg aaagtaaata cgttgcgcct
    15061 gaacgtaagc tcacacttgt ttgctgcgtt aaaccgccaa ttatagaaat aatcgtataa
    15121 tagccttgat ttaaagcaag actcatttta ctacctcgtt gcttattcac ctcagataca
    15181 agtacgccat cggaggttcg cacaaaaaat cgcccttcga tttcagcagg aaaaacaata
    15241 attgattcag agtcggtgat atcagtaaga atgagatccc ctgagccaac aagggtaata
    15301 ttatatgacg gatgttgcgg cccaattgtg cttcgttctg tttgcaaaag tgtttcatta
    15361 aaagcataat gatacagctc gttgagcgac accttattat ctcctgagct atcagctgca
    15421 cctcgtaaac ctgttatcaa agcatgtgta aaaaaagaag cttgtataag atcagattct
    15481 tgcgaagact cactttcaga gcttgaggaa agataggcat ggccttgaac caccgttgag
    15541 tcatcaacca aaaaagattt ttgcttttct ccaccctttg cgcgaataaa atttcccgaa
    15601 aaacaagagt caagcataac aacatgcaca tcgcttggaa cagcagaaat acttgctttt
    15661 aggtcaacat aatcataacg ctcgtttccc aaaagcaagg cgttttcatc agaatgccca
    15721 gaataataga agacaaactc cgtgcgtttt gtatgacttt tattttttac aactttttca
    15781 gataaaacac gtaaatatct atcaagctct tttttacttg ggtcaagtaa aatatttgaa
    15841 ttttctactt tcacaccacc cacgtctatc atcgtttgtg caagacgatt tgcatctgtt
    15901 ccagcataac ggagtatttc acggcctttt cccccatcat ttgatgcaac ataaatcgcg
    15961 tagcgctcta tgggtaaatt ctcctgcgca aaaaaaacag ctggaaggaa agacaataaa
    16021 aacacacaaa taaagagggc aggttttgct ttttttctgt atttcaaaaa caatttcata
    16081 ttattttcct cactttacta aaaacacatc atgcacttct aaggcactcg gtaaataaga
    16141 ggcaaggttg tcgattgagt cccttgcatt gaggtctatt tgattaaaga aagcatccaa
    16201 attttttctc gaaaaagaat cctttgagga aataaagaaa aatcgctcaa aagacggcgc
    16261 attgtcaagt ttataggcat aatcaagcgc tatctctcct ttacctgtaa gtagacctgg
    16321 ttcttttcct ttttcgggaa aatgctgagt acaatggcca ttaccatcta cagaaaaaat
    16381 atagccatac gaaaaaccgc tagaaacata gcttacttgc aaaacatcac cttcgctaac
    16441 ctttgaaaga tttgcaagac gtaccgctga agaaccttct tttttataca caaatatttt
    16501 tccgccaaat ccttttgctc gctctgagcc tttcggctcg ataaaaactc tgttttgccc
    16561 ctgggtttct gttcggtttt taagttggat ccctatcgga agtaaaaatg caaggcaaag
    16621 cattgcagcg atggccataa ttctttgcga tgtaaaaaaa gccttcgtct cgttaggccg
    16681 aaattttttt tcaccaccac gaagtttttc ttcctttgaa gacagcatct ttgaacgaac
    16741 agcaaactcc atgtcatatg ctggatattt ttctaggata gccttattct ctttttttat
    16801 agcatttatt tcttctttct gttttatttc aaaatcattg tttttatata aaacttcacc
    16861 gagtataatt tgttcaagat ataagtcttt tttcctcatt tttttcctcc aacgttttac
    16921 aaaccttctt tattcttctt gatgctttgc taaagctttt ttccgtaaaa tcaaaagtcg
    16981 ttttcgtact ccagaaacag agagccccac ttctttcgct gtttcttcga gggtaaagcc
    17041 gtcaacataa tgcatgagcg ccatgtcctg tgtacttttt tttgtctcat caaaaatcgc
    17101 atccaaaact aaactcgcat cgattatatc ctcgtggttc gctcgataat tgtcttcaat
    17161 ctcagtaata aaactttcga tatcaggagc ccaacggact gaatcatagc gaattttatt
    17221 taagcagaca cgtgttgcca ctgtgtaaaa aagactcgaa cacacacttg tgattcgctc
    17281 ttttcgctct aaaatacgaa caaagacatc ttgcatacaa tcaagtgctt tttcttcatc
    17341 tttgagtaaa aaacgacaac gtcttagcac catagggcca aatcgttcgt agagatctgt
    17401 aaaatgttca atattcaagt actcttcttc tttattcacc cttattcatc cttcagtcat
    17461 actatataac accttctcgg taaaaatatg cactatttta attataccat tattaagaaa
    17521 aaaatcttgc ttttattatt tattttgatt attatacgat tatggatatt atcttacaca
    17581 gtgtaactat ttttgccttc gtacttgttt tctttgaatt tttctctttt ttctttcgaa
    17641 agaaaaaagc gctcaaaaaa ctcggagaaa agaaaaaaaa cttactcgca aaacactcct
    17701 tctcttttct caccctcttt gttctctctc ccatgctcat cggcttagtc tatgtaaaaa
    17761 aaatagactt tttcccaagc atcatcctct acgcctgtgc gattcttgcg cttgagatag
    17821 caatgcgtga ttttacaaca aaaaaactgc aaggaatcta tgaaaaaggc tttgtgatta
    17881 acaataaaat tatcctttat gcagatatta tcgattttaa tccttttgca ccgaaaaata
    17941 acgacgattc agacatagct gaagatgagc agtatattgg aacaagtcgc ctcgacattg
    18001 caacagaatc aaagggactt atctttgtcg attttagctc gactggtgaa aaagaccaat
    18061 ttctcgactt ttacaaaaat catgtttcat caacataaaa aaaaggaacc gcctcgaggc
    18121 agttccttct atataaaaag agcccctcgc tttgcgtaga gtgctcgaaa aaaaagcgtc
    18181 ttttgtaaac aagtgaacca agctacaaaa aacgcttaaa caggcgccga tcagaatcga
    18241 actgatgcat aaaggttttg cagacctctc ccttaccgct tgggtacggc gccaatctcc
    18301 tataaatata ccaaatcccc ttttttttgt ctagcctatt gagataaaac agtatataat
    18361 taacaaatgt ttaagattga gccacatata gagttcgcgt cagataaaaa cagcgcgtat
    18421 tacagtcttt tttggtcgca actcgaaaaa gcagataaat ataaaataac tacttctgta
    18481 ccttctgtct ctgggcttta cgaaatatac tatgaagacg cagaaaaaag gctaaatctt
    18541 ctcaatgtcg ccgctgcctg gcttggaggt ttacgttcac aaatccgctc agatatcgac
    18601 agtgacggaa aatctgatga agtaaaagaa attttagaaa catatagcct ctattatcgc
    18661 tatacgattt ctccttcttt cgactccatt aaagatgttg cctggtttct aaataaaacg
    18721 tattttaaag atgcatcgac gagtcaacat tctgggcgct ttgaaacaat ttatgtgaaa
    18781 gaatttgcac cagataaaat acgctgggtt tagataccgc tatgtcatgc cgtgacgaat
    18841 catactcgta caaaaacaag gaggctagat atgaaaaaag ttgattggga ttttataatt
    18901 ataggcgctg gaaccgcagg tcttgctgca gcacaatatg cagctcgttc ttttatgcga
    18961 acccttgtca tcgaatctga cctctgcggc ggacaagctc tttttattta tcagttagaa
    19021 aactaccctg gactctggcc agcggtttct ggtggcactt ttatagaaaa tatgaaaaac
    19081 caagcaacga cctttggcgc cacaatagaa aaaatgcaag taagctctat agacaaaaag
    19141 ggtgatgtct tttatgtaaa atcagaaaac aaaacaataa caagccatgc actcctcata
    19201 acaacgggcg ccacacatcg taaacttgga gttccaggag aagatgcgtt ttattcaaag
    19261 ggtgtttcct actgcgcaac ctgcgacgga ccttttttta gaaataaaaa aattatcgta
    19321 atcggaggag gcgactctgc ctgcgatgaa gcaaattttt tatcgaacct ttcgaaagat
    19381 gtcacactca tccatcggcg cgatgagttg cgcgcacaaa aagctgttgc agatagagtt
    19441 ctgcaaaatc caaatataaa agtactgttc aataccattg ttacagaaat aaaaggggag
    19501 caagtagtac aagaaattat catccaagat gtccttacaa aagaaaaatc gagccttgcc
    19561 gccgatggtg tatttatttt tgtaggcatg gatcctcaaa cacaactgat aaatagcctc
    19621 cctaaagatg agggcgggca tattatcact gatgaatata tgcaaacagc tataaaaggt
    19681 atgtactgcg caggtgatat tcgctcaaaa ccatttcgac agctcatcac cgcagcgtcc
    19741 gacggtgcta tcgcagcgca ttgtgcagga aaatacattc gagatgaact cggggagatg
    19801 tgttgctaaa tgtccttgct taaaaaaagc cttttaagcc ttcttttact cagctctttt
    19861 tttatcattg caagcacctt ttctatcaag caagaaacac agatcataac agaagaaatt
    19921 accttttggt cagagataga acacgaaaaa cttgccgaac tcctcgtcat acgcatgaca
    19981 gacgaagaac ttttggcgca gatttttatg tttggctggg caggaagcga gccctctccc
    20041 ctgcttttaa gttggatacg agaacgtgct cttggtagcg taaaagtttt tggttggaac
    20101 acagacaata ttgaatctgt tgcgcgtgcc gtaacacaaa cacaagaact ttcccaaaaa
    20161 aaccgcctta aaatccccct ctttgttgca acagatcaag agggtggttg gattcgtcat
    20221 ataaaaggtg acacaagcga cacgcctgga aatctagcca ttggtgcggg aggcttgccc
    20281 atcgacgctt atctttcggg ctactatatt tcaagagaat tgcgcgccct tggcataaat
    20341 atgaattttg cgccaacaat tgacttatac acaaataaag actcttctgt gattgggccg
    20401 cgctcctttg gagaagatgc ccagcattca gctattttgg gacaagcctt ttgccaagga
    20461 agcatcgacg caggaattat ccccaccgca aaacattatc ccgggcatgg cgatacaagc
    20521 gacgactccc acggctatct tcccaaaatt aatatttcaa aagaagtcct tctcgacaga
    20581 gaactcgtgc ccttcaaaca tcttatatca aacaaggtac cggctattat gtctggacac
    20641 ttgagctttc ctgaaataac aaagtcaaac gaacctgcgt cattgtcgcc gtattttttg
    20701 aacagcctct tgcgagagga actcggttat acaggtttaa ttatcaccga tgatatgatg
    20761 atgaacggtg cgacgagcta tgcgggaagt ctatcgcgtg cctttcaact tgctatagaa
    20821 gcaggaaacg atataattat ctcatcaacc tgtccgcaat tgaacgaagc gctatggact
    20881 cataatctta aacgcatgaa ggagaatgcg acttttaatg cgatcgtaaa aaaagcagca
    20941 aaaaaagtag tgtatgaaaa attagtgtat tttaaaagcg ataatgctgt tcccctttat
    21001 cccgatatcg aaaaaataaa gcagaatatc cccgaccctg atggacaagc tttttttcta
    21061 gaccaagctt gtcgaagtat tacccttaga aaaggcgata gtattccgac tataaatagc
    21121 tctcaagata aaattgtcct tgcaagctcc tatgcgtctt tttttaatct tggtctcaag
    21181 cgctatccac aggcaaaacg ctaccaattt tctcaaaaca tggggccaaa tgaaactgcc
    21241 tatacaattg aaaaactcgg cgatctggca aaagacgctg attccattct tttaaccgta
    21301 gcaaacgaac gagatgctga agtagcaagg gctcttgcaa aacttttttc tgagaagaac
    21361 aaagccagcg tcgaagcgca aaaaaaactc atcgttattt cacttttatc accaacacca
    21421 gccctatact tggaaaatgc aaacgttatc tttgcctaca gctactcccc ttattctttc
    21481 gaagctgttt tttccacact cgaggggcag tttagcccaa agggcatact cccttttaac
    21541 gattatatgc agccatgaaa aaaaacatcg agccaaaact aaaaatagaa ttgagcgctc
    21601 tttctaaaca aaatctagga gaagtatgga actttctaaa aaaacatgaa aaaacctcca
    21661 tcgcactttt ttcacatctc attgaaaaca ataaagcatg tttaccaaaa gagcaatttc
    21721 gctatttttt agtttatcaa aataatgaaa aaacaaaaaa attacttgct gtttttatga
    21781 tttcaaaatc aggacttatc tttcatcaat ttgctgataa tctaaaaatc ttagaagatg
    21841 ctgatatcgc tccgtttgaa gatatactca aaaacaaaaa catctattcg ataatcggga
    21901 aaaaagaagg gacagagttt tttcaaaacc tcatcttgaa aatccaggcg atagctccgc
    21961 gcacagaata tttttatcat tatttgcgat ataataaaaa cgaaaaaaaa acagaggnnn
    22021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncaa ataggaggcg tttgtacaaa
    22141 aaccgatgag cgaaatcgcg gttatgcaca gtttttagtg caaagtcttg ttaattattt
    22201 tcaaaaaaaa gagaaaaaac aagttgccct ctttgtaaaa gaaaataatg aaagtgcaaa
    22261 aaaagcatat tataacctcg gctttgtgtt tgattcttac ttcaaaattg tgtattattg
    22321 aaagatatga agtatattga atttttagat ctaagctttt cttaccccct tgaaggagaa
    22381 gcaaaaaacg aagaccaaaa tctcgatgcc agtctaaatg aaaaagaaga tataaaaaat
    22441 atctttgaac actttactgc tgaactacct ccctcttttg taagccttat cggtccaaat
    22501 ggttcgggaa aaacaacgct catgcttctt gcaagtggca gacttcttcc aacgagtggt
    22561 acagcccgcc ttttcggcat agatacaaaa acactgacaa atgaagaaga acgaaatctt
    22621 ctcgcaagct ttatctatca aaatatggag ctcgaaacgg atgaaactgt aggcgacttg
    22681 cttcagtcta tttttgaaaa tggacaacat ccaaaagaaa atctctttct tgttcaggaa
    22741 gcaatcgatg cgctttctct agaagcgagt ttagaaaaaa aactcaccgc tctttctaag
    22801 ggtgaaatgc aacgaagcct tattgccttt tcaatccttt atggctcgcg atctattttt
    22861 atggatgaac ctctatttgc cctcgaatat acacaaaaag aagaggcact ttcatacata
    22921 caatcttact caagacggtt tcagataccc gtttatattg cgatgcatga actcgaattg
    22981 agtcgaaaat gggcagacaa tgtcttattg atttttccca accgtgatat gagcttcggt
    23041 agtccagaag aagtactgac tgcagaagat ttagaaaaag cctatggtat acctgcggca
    23101 atgctcaagg aaagtgagcg tttaacgcaa agaacgcttt tagaattaca aagcgaaatt
    23161 gaaaaaaaca aagacatacg agcgtaaaaa aaaatgaata ttgttttatt tagcgacgag
    23221 gaaatacttg aaccactttc tctttttgat gaacgcgccc aacatatttt aaaggtgctg
    23281 aaaaaacaag aaggtgatta tttcgaagct ggcattatac acggaatggc tggcaaggct
    23341 catattcaat gcattgatga gcgcggcatt cacttccatt ttaccccgag cagtaatggc
    23401 aagccactct atcccgtttc tgttatcatt ggctttcctc gaccaattca gttaaaacgt
    23461 gccttccgcg acatggctgg tctcggcgtc agcgatatat ttctcgtggg gacagagcta
    23521 ggagaaaaat cctatcttag ctcacgcatt gtaaaagagg gcgctgcata tgaggctctc
    23581 aaagacggtt gcgcacaagc aaaaagcact catattccga acctctctgt ccatgatgat
    23641 tcagaaagct gtttagatta tttagcaaat caaaacaaac actatcaaaa cccttcctct
    23701 ataaaaataa ttctcgatcc aaataagggg aagggttctt tacaatcgct ttttgcacaa
    23761 aactatggcg cagtcgatgc tgttttagat tattactata tgggacaaac agatacagca
    23821 cctcatattt ttctttcgat tggaagtgaa cgagggtgga ctgaaaatga attgaacatt
    23881 tttcttaagc actcttttgc accctgtctt ttaggcgaga gaattttacg aacagaaacg
    23941 gctgcaacaa ccgcagtgag cataattctt tctcaactgg cgatgctatg aataacgaaa
    24001 aaataaaaca aatcttgctt tctatacaag aatcttccat agaatttgcc gttacaatga
    24061 gtggaaaggc aagcaaaaaa gtaaatggct tatataatcc tgaaacaaag gaaataattc
    24121 tccacaataa aaactttcaa aacgataatc aactgatata taccgcaatt cacgaataca
    24181 cccaccatat tttaaacgaa gaattgcttg aacggacgca gggtttggga aaaatgaaaa
    24241 gcgcacgaag tcatactaac gaattttggg cacgttttca tagccttctt gaagttgcag
    24301 aaaaaaaagg tttttatacg attgctatcg aaacttctcc agaactccaa gccttaacag
    24361 aaaccataaa aaaagattat ttagaaaaaa atgcgctcct tatgagcgag tttggaaaac
    24421 tccttttaga agcacatgcg ctttgtctca aggaaaacat tcgctatgaa gattatatcg
    24481 atcgtgtgtt acaaataccc cgaactgagg caaaaaacat tgtaaaaata agctctgcaa
    24541 gttttaaacc cgaaatcggt tttgaaaaca tgaaaatgct tgccaaaatt ggaaacagtg
    24601 agaaacgaga ggctgcggaa tcgcaattgc ttcaaggaaa aagccccgat tctgtaaaat
    24661 cctttgttag agcgagacca gaagccctcg acataaaaga agaacttata aaagaaagag
    24721 gacgcattga aaaaacaata gaccaattgt caaatcgtct cgatcaaata aacgcaaacc
    24781 tttctgtact ctaattatag acaatctttt ttattgccgc gcaaaaaaag atagaccatt
    24841 gcatataaac tttcacttcg aacaggcttt gttaaatact cattcatgcc aatactttgc
    24901 gccagtttta tctcttctgc aaaggcatct gcagacattg caacaatagg gatttttttt
    24961 aaatcttctc gagtagaact ccgtatcaac tttgttgcct cgcgcccatc cataactggc
    25021 atgcgaatat ccattaaaac aagactataa taagtcgcct ctgtttcaag caatcggtca
    25081 accgcttcct taccgtcatg tactatatct accgtcaaat tttttctttc aagcatcatt
    25141 cgcacaatat ctgcattcaa gtcattatct tcaacaacga gaattttttc tttattgtac
    25201 atctcttcta tctcaccgat agaatcgctt ttacgacgtt tttccttcgt tgcttgcaaa
    25261 atcttaagcg gaacagaaaa taaaaaagca ctgccttcat tcggggttga ttcaacctga
    25321 acagtcccac ccatcaaatg cacaagactt ttcactatag ataaaccaag cccaaaactt
    25381 ccctttgttt ttatctttgt cttacctttt tcaaaaggct cccaaatact gcgaagacgt
    25441 tctgactcta ttccctgccc tgtatctttt accacaaagg taaataaaga aaaaaccgat
    25501 gctctttttt cttcaaaaac agtcacttcg atttttcctt tttccgtata ctttaatgca
    25561 ttatctaaaa gatttataag tacttgttca atatgttctc tgtcgccata gacataatcg
    25621 ttttctattt tgtttgtgcg tataagaagt tgaatcccct tgtctttggt ttttccctca
    25681 aataacgagg caatagtatc cataacagaa ctaataaaaa aactatcgtt attgatcact
    25741 gtttttccac gctctatccg tgacatatcg agaacgtcat ttaccaaagc aagtaaataa
    25801 tctgaagcag aagaaatttt ttttagcgca tcgaaaataa aatcctggtc agaaacttta
    25861 tcaaaagaca ggtcggtcat accagttatg atatgaatcg gagtacggat ttcatgtgat
    25921 attcgagata gaaaatctgt tttagcccta tttgcttgtt ccgcatagct aaaagctttt
    25981 tgcaattcat gatttgcctt tgaaagagct tcatttgccc ttctccgtct aaagtttatg
    26041 cttgctaaaa aagcaagaaa aacaataaaa attagcactg taaaaactaa aatcataatg
    26101 agaaaaaaat gtttatataa aaacgtttgt atagtatcgg gctcttttaa atacaaggta
    26161 ttattgtata ttgcattatt tatctctatt tctgtcaaac tattaaccgc tttgtttata
    26221 atcgagaaaa gtaatggatc gcaattttca tcgagtgcga atgaaatttt atagggcata
    26281 tccgcaagag agtaataaaa caaacgagca ttttgtggtt tttgtagcaa tttttcagca
    26341 gaataggttg gaataaaggc aatatctgct tttttttcac gtatcgcatt gacacattct
    26401 tctacacttc ccacaatgat ataattctta tgcggaagca catatttttc tgttaaataa
    26461 tacccatcta ttccaacgaa ggttaaagaa tccttgtgta tatcttcatg atgttcgcgg
    26521 actaccgcac taacaggtaa attcaaatag gggtttgtca ttatcatttt atgtttgtca
    26581 gcccatgcat aatcatgtgc aaaactcgct aaaataatat tttttttatc cgctaatata
    26641 gggaaaaaat ttttgtattt gtctgacgaa atatacgaaa aaactatgcc tgttttagct
    26701 gcaatataat caaagataga gggagttatc ccttttgcct taccatcttt atcttcataa
    26761 tcaatgggca tccaatttgt ttcatgggca acttgtataa cgggattttc ttttatatat
    26821 tttttttctt cctctgttaa tgataaaact gcatccattt ggttttgata gtaccttgca
    26881 taaagcctat cttcaaaaga gggtttttga agaaacagtt gatcatgcgc ataattaaac
    26941 tcagctaaaa tgtctggacg cgtttttgaa atagcaaaat aatgctcaaa ggaattaaat
    27001 tttctaagca tctgttcgcc agaaaattcg cgtaaggaag tgctcagtat ataatctatt
    27061 tcttttgtct catgtaaggc ttttatcaaa tcgctataat gttcgtaata cataaaaaca
    27121 attggttgtg gaaatagatc ccgaaacaaa tccgaaaaat ctttttcttg aacagctcct
    27181 aaacgcaaac ctagttttat cgtttttcta ttttcaaact cgtcgttatc gacaagcgta
    27241 gtaatagttt cataaccatg accactgcta aactttggga aaaaatatag ctcttctcgt
    27301 tcttttgttt ttcgaatgct tcctacaatg tcgagttctc catttgcaag catctctaag
    27361 gctcgactat aagagagttt ttcagttctc gaagtcggtt gcccctgagt atcaagaact
    27421 ggaactctag taatatattc aaacttccag tctgtaaatt tctgtagttc gcgtaaatat
    27481 tcatagccaa aaccagagag gtttccatct tcgattatgt ggaaaggttt atactcaaaa
    27541 aagccaactc gtactgattt tgcaaaaaga acaaaactga aagagaataa taaaactaca
    27601 aaagcaaaag gtctttttat tttcatatat ttgttctccg caaagataaa agccctgttt
    27661 aaaaaagcta aacttcttca actgttttta aaacacattc tctaaaatct gcttcaactt
    27721 ccctaaaaca atcaaagatt ttatccgaaa aagagccaca ttcgccatca taaatcattt
    27781 gtacggattt ttcatgacta taggcttttt tatacactcg atcgctcaca agtgcatcgt
    27841 aaacatcggc taaagaaaca atttgtgcaa caaagggtat ttcctcacct tttagaccgt
    27901 caggatagcc atttccgtcg tagcgctcat gatgagatcg acaaatatca taacaatagc
    27961 cataaaactc tggatcgttc acataggcta attttgacaa aatactacaa ccaataagcg
    28021 tatgattttt catcaagtca aattcttcgg gtgtcagttt tcctggtttg agtaaaatag
    28081 tatcaggaat gcctattttg ccgatatcat gcatagcaga agcatttaca atttttatct
    28141 tgctttgcat atcatccaag acgatatagc ctttttctat agccttgtcc aaaagcaagg
    28201 aaacaaaggt ccgtatcctc tgaatgtgat agcctgactc caaatcacga aattctacaa
    28261 ccgtacttaa aacatctatt acggactcat ttattttgcg taaatgcaca ttttgctcgt
    28321 gaattttatc ggtttgtata gcaatcatag cctctaattc atttttatga agatagaggc
    28381 tgagtacgtt ttcaacacgt cgacgaacga tatacgggtc aaaaggtttt gttataatgt
    28441 ccgacacacc taggtcataa gaaatttttt cgttttcttt actcgtagaa cctgttatca
    28501 gaacaacggg gatagtatgc aaatcaccag tacgagccat ttcttttaca acatcaatac
    28561 ccgtttttcg tggcataata atatcaagca gaactatatg aatagaatta gcatgttttt
    28621 ctaaaagctc tatagcttcc tgcccatcgg atgcgattag aagagaatac tcttttttaa
    28681 aaatttcaga taaaatgctt ctacttatct catcatcatc tgcaattaaa atagttctat
    28741 tcatgctatc tccttcgcat acgattataa cacatttttt ttgactatgc aaaataaaag
    28801 ctctgaaaat cagataaatt cagtactaac aaaaaaagcc ccttcgataa aaaaggggcc
    28861 tagctgttct tcatttttta ccaatcaagg agggttcgtc tgccttcaag actacgtatt
    28921 tctgttgcat ccatactcat ttcaagagta ccgagatatt ctccggcttt gttataaatg
    28981 ggcttatatg tgatataaat aaattttccg tttaattcaa gccaaaaaga tgcatcggtt
    29041 ttttcttttt tcttaaaaga gtcgagtatc ttttcaacgg tttctacgct tttatgtgga
    29101 tggcaatttc ttacatcgcg ccctataact ccaggacttc gtggaaatac acgatgcgga
    29161 ttgtcagaat aatataaaac cttgtcatct gcatctacaa aggaaaaatc tacaggcaat
    29221 gaggttaaaa tcagattgag cacttcgagg ggaagtttgc ccacatcgag attgatgagg
    29281 gaggcgggat tttgtacttc agttttttct gttaagctcg aaacagcctt gtcaaagttt
    29341 gcatacatcg aagttttttc tggcttaacc aagatggggt cgtactctgc tccagggcga
    29401 atccaagcaa aaccgatcgc atcttcacct tttcgaattt ctgcccaatc agcttcgctc
    29461 aataaacgtt ttgcatttgg aaaaagtatt ttttcttcca taaaaaataa ggcttttatt
    29521 tttcctgcta gaactcgtgc tatttttttt gcctgagcac tattattttc ctcagaggcc
    29581 ttatcaaaaa gcttaaattg ctcgcgaacc tcatcgtgtt ttccccacat aacgcgtgag
    29641 gggccagtaa aattcttttt ttcaagatag ggaaaaagtt ggttttcttt ccgcgtataa
    29701 tgcgtaataa tcggcttcag ttcttgcacc gctgttttta aggcatcaag acttccaaaa
    29761 agtcgcgttc gattaagtgt ttttactttt ctgcgagcaa ttttattttc agctagatag
    29821 gtatgaatcg gatgtcccga aatctgtttt atagagcttc ctttttctaa gccagctcga
    29881 aataaatcag catgcacctc gcatagtttt tgtaactcat ctacaggaag tccgtcttct
    29941 ataagtgact gctccatggc agcaacttct gcagcatcag tggttttaat aatatcatga
    30001 aattcttttt ttactgcttc tggattttct cctgcagtta aggcgtcgat aaccgctttt
    30061 aaacgttttg tttttacagg atcgacttca aaggtttttg tttttattag ttcactcatc
    30121 ttttaactcc tactaaatca gtatgatata aaggtaagct ttttttcctt ctttgtcaag
    30181 caagttgcta gcatttattt ttcgaataca tatactagta ttatggatac aaatacgaag
    30241 caaagaaaaa caatagcaat cagtatggga gaccccgctg gcatcggtcc cgaaattatc
    30301 gtaaaagcac tctttctcct cctcgaagaa agaagggcaa agtcagaaac ccttgcccaa
    30361 tgcgctatcc acatgaaaac atcttcttca aaatttcttc tcgtcggcga ccgctctgta
    30421 cttgaagaag cttgcgaaat aacagatatt ccaaaaaaaa tactctcaga tcccgacctc
    30481 gagattatcg acttaaacaa tgcagctgca aaaagtttta cgcatggagc tgtttccgcg
    30541 ctttgtggca aagctgctta tgaatatata gaaaaagcaa caagtctttg tatggaagga
    30601 aaagcccatg cccttgtaac cgctccgatt aacaaggaat cgctccgtgc gggctctgtc
    30661 cccttcatcg ggcatacaga aattatcgca gaacttacaa acacaaatga tcccttaacc
    30721 ttatttgaag tgaaaaatct tcgagtcttt tttttatcgc gtcatgtttc cttacgaaaa
    30781 gcatgcgact tggttaaaaa agagcgtata gtcgactatg tagtgcgttg cacaaaggcg
    30841 ctcaaacaac ttggcataga agaaggaacg atggctattg caggactcaa tcctcattct
    30901 ggggaaaacg gactttttgg cgatgaagaa gtgagcgaaa tcattccagc tataaaaaca
    30961 ctccaagaaa tgggctataa ggttgccggt cccattggtg ctgactcggt ttttcatcaa
    31021 gccttagaag gcaaatacaa tagcgtactt tcactctacc acgaccaagg gcatatcgca
    31081 acaaaaacct atgactttga acgcactatt tccataacct gtggcatgcc aatactacga
    31141 agctctgtcg accatggcac cgcctttgac attgccggaa agggcattgc aagccccata
    31201 agtatgatag aagctattaa agttgcagaa aaatatagct agttcgcaag cctaaaattg
    31261 cctcgctttt tccaagccta aaaacttcag actagcaggt tttgtttctg ccattttttt
    31321 gcgagatttt ctggcttttt tgtaagcaat ttcagaaaaa aatcgtccat ttgagcaaat
    31381 acttgtccaa gaataatgct ctcatcgtca gtaaaatcgg ataaaaccca gtttgataca
    31441 tctccgtgaa ccggtcgacc gattcccaaa cgaaggcgcc aaaaatcagc agttcccaag
    31501 cattcctttg ttgaacgcaa accattgtgc ccacccaaac cgcctgccca tttaagactc
    31561 actgtcccca agggaagttc aagctcatcg tgaactacga ggagattttc tggttttaac
    31621 ttataaaaac taagcagggg tcctatactt tgccctgaaa gattcatata ggtttctgga
    31681 aaaagaaaat acaagggctc ttcgcgatcc gcaaaatgct gtatactgcc catttgacca
    31741 ttgaactttg tttgaaaata tatatcagat aaaaacgaaa gatttcgtgc aaacaaccag
    31801 gcaatattat gtctattttg ttgatatttt tttccatgat ttcccaaaaa agctaccgcg
    31861 ctaatcatca aaaactcctc atctaaaaaa ctacaaaacc agtatagcga aatcattcga
    31921 catacacaag tttagctaaa aaatcgtata tcctagcttt ttgcttgctt tttttcctgt
    31981 ttatgaataa attaaataga tgaataaaaa tacaaacgat aagacaaaag aaaatgcgaa
    32041 aatccctcag tatccagaaa aaatcaccct ttcttgcact ctcgcaaaag aaatgcaccc
    32101 ctttatgaaa acactttcag atggaatctc agagttttct tttcccagcc tcttccttca
    32161 tagaaaaaaa tataattatt cactgagcaa agtttcggac gaactgtatc ttctctttgg
    32221 agagcaaaac aaaaaaagtt ttttttctgt cctcggcaac ttcatcgatg caaatcaagc
    32281 agaagcagaa tcacttttta taaaactcct tgaaaatggg ctgtattgga aaaacatgag
    32341 tgaaagccaa aaaaatgcct tgagtccaga ttttttatca cgtatcgagg aaaaatacac
    32401 cctcatcgac gatcgcgaca atgccgatta tatatatctt cgagaagatt tagcacaact
    32461 tgtgggaaaa acttatcata aaaaacgcaa tctcataaat gccttcgagc aatcctacac
    32521 cccgcgagtt gagcctttga gcgaagacaa tctcgcggac gccttccata tcttagaaga
    32581 atggaaaaac aatacaagcg aaacatttac agactatgag caatgtgtag aagcactcaa
    32641 tagcttaaaa ctcgataata taaaatcttg catggccttt gaaggaatta tcgtatacgc
    32701 tgacgataaa cctgcggcat ggacacttgg cgaaaaaata gcacaagaca gaagctttgt
    32761 cgtgcatttt gaaaaagcca tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32881 naatcaaaaa gtaagctttg cagaagcaat attaaagcct gttcccaaag atggcggtat
    32941 gtatgtacct gcgagtgaag ataatttacg tccttggatt ttacacttag atgcttcgag
    33001 tagcttttct tctattgcag gctctcttac atcagctctt ttaaaagacg aattcagccc
    33061 ccttatttcg gagaacatcg ctcaaaccgc ttttcctttt agccccgaac tcaaacaact
    33121 cgataaaaac ctctttgtgc tcgagctttt tcacggacca acgggaagcc ataaggactt
    33181 tggcgtctca tatcttgcaa gctgccttga gcatattttg cacatggaag acaaaaaagc
    33241 aacggtgctt gcggtaacca atggggaaac tggcgcttgc attgcaaatg cttttcgtgg
    33301 caaaaaacac cttcgcgcgg tactcctgta cgcaaaaggt acagtacgag gctttcaaga
    33361 aacagatgtc ctttcacaag gtggcaatat tatacccctt gaaataaatg gcagtgaaga
    33421 agattgcttt caacttgtcc gcgatatgta cgcagaccaa gacctcatcg aaaaatataa
    33481 tctaagcctt gcaaactcgc tcaatattgg gcgactctta ccacatagtt ttttttatac
    33541 ctacgccttc tctcgattaa aaaaaatagt acatagcgat attttttatg cgatgactcc
    33601 cggaaactat ggtaaccttg tcgccggctt atatagctgg aaattctctc ttcctgtaaa
    33661 tggttttatt gcaacaagca cccctgaact ttatgacgac atacaaggga aatgcgctgt
    33721 atctaactca catattcccc ttgaaaaaag aaatgtctct gacccagtaa acccctcaaa
    33781 catagaacga cttgaggaaa tcttcgcaca caatccccaa gttattcgcg ccatggttta
    33841 tccagcgaag ataagcgaat ctgaaactga agtcgctttc aaagaactgt ataaaaacta
    33901 caaggctctt ttagaccctt caacagtttc ggcttatgcc gcagtaaaaa aaagacagga
    33961 tatcatctat gccgatgacg gagttgcggt acttctttca cgctaccacc cctctttttt
    34021 tgctgatcaa gcgcgcatct ggggagcaga aaaggtaaaa atgcccgaac agcttgtgga
    34081 aatgcaaaaa aaacaaaggg cagagcattg cattgaagca aacaaacaag cccttgagca
    34141 aatactaaaa gagagcacat aaaagaggag cattcctatg cctgcacaaa accctgctat
    34201 acttgaaata catagattgg taaataaaat aagcttttat accgaaatga tttactccga
    34261 tgcactttta ttagaccttc atgagttttc tgaatactgg aaagatgcac tcagccaaat
    34321 ttctgaaata aaaagagcga ttaaccataa attttcatcc aagacggaag acagccttat
    34381 agataccgca attaaagaag aactttcacg gcgtattttt ttatttagca gaattatcaa
    34441 ttctatccct gcagattttt ccctacacaa ttttcctgaa atagaaaccg acattgatac
    34501 cgtgcaagcc ttagtagaaa cagcacaaga ttattttgaa aaaattttat ttattcgctc
    34561 ggagcatctt cacaatatgc ccatcgaagc actaaaaagc gcttctgatt actccttgac
    34621 aaaatactta acaggtatgg ttgatcgcgt aaaaaacgca agcgccggaa caattgtttt
    34681 tgcaggaaag cttggaaatg aaacagcaga aatacaagat ttattagcaa cagcatcatt
    34741 tcataccata aatatcctag aacaagaatc tatttacgac attatctcaa cctatgacgt
    34801 ttcgcttatt tgctatgatt gtggtcaaaa tactatggaa ggttttagcg tcttacaaga
    34861 acttatcgac gaccctgttt ctgcagatat acccgttctt atttgtggct cagaatacag
    34921 cgacttactt gcaatgcagt ttattgaagc tggagcaatc gattattatt caacatcgcg
    34981 aagtaaaaaa acacttttcg cacgcattca agccgcaatt aaacgaacac aaaacaatta
    35041 tcacaggcag ctctatatcc gtgcccttga actaaacaaa caaagcactt caaaagaatt
    35101 tactgcggct tcccagtaca ttgccgactt attacctcga aaaacagaaa acgactattt
    35161 ttctataaac tggtcttttt taccatcctt acaactcggt ggcgacatct ttggctatta
    35221 ttggctggat gatgagcatt ttatgctcta tctactcgat gtcagcggtc atggactcga
    35281 agcctcactc tactctgtta caatcatgaa tttattaaat aacgagttac ttggaaccgc
    35341 aaatttttta gacccatcga gtattttgcg tgagctaaat agaattttcc ccattgaaaa
    35401 acaaaacaat atgtttttta caatctggct cggcgtatac aatatccatt cacgaaaatt
    35461 agtttacagc aatgcaggaa gtcaacctgc tatacttatg atcgataata aaccacaccg
    35521 actcgcaaca aaggctacaa ttattggtat tgatgaagat tccgagtacg aaaatgcaga
    35581 aatacaggta ccaaaaaact cagacctcta tatttttagc gatgggattt atgaaataaa
    35641 aaaaatcaac aaagaaatga tgcatttgga agagtttatt gcatttttag tagaagccca
    35701 agcatgtaaa gataagaatt gtcaaagctt tattcgaagt atagaaaaac aaagcattac
    35761 cggaggtttt gaagacgatg tttctctcct ccacctccat tttaattaaa aactcctagc
    35821 ccgaaacctt ttattttcga gctaggatct atcctaagcc tttttatttt tttacatcct
    35881 tagtttgttt tttactcggt tttgcttttt cctcttttga ctctgttacg gcactatcca
    35941 tttttgtacc caaaagaaca gcgagcacct ctttgtcata gtactttgaa agcagttttt
    36001 cagccgtttt tttatctttt ggttcaagca gttgtttaaa tgtatctgct ttataggatg
    36061 catcgctcaa ggcctttaca gaaatagcat acaaggataa taagcgttca tagtccttgg
    36121 gttttgcggt aaataaggca tataacacta tcgctgccca aaaactaaac tccgcttttt
    36181 ctttttgaag ccagagtaca tcagcatagg aatttgcacc tagaatcaga gaagcttcct
    36241 ttgtttcgag tactgcttcc acaaaaagat aggctgaaag ggaaagatct tcatcgatta
    36301 tgggataatc actaaaacgc gtaacctcga taaatgtttt aaaaatctga tacatgtctt
    36361 tagaagggcg tccatagctc acaagtaaat cacgtatttt tctgtctaaa caccaaagat
    36421 taatcaattc tttgctatgc caggcagtcg ctttttcacc gatcaaatct cgcacggaat
    36481 acactatcgc ataagagaca agcaattcgc ccatataggg atgcgctaag ataccgtcgt
    36541 atataaactg cgaaagggct tgtaaatgag cctccttgac tgtttttttc tgtgcgcttt
    36601 ctacgaaaga catgagattt tgtattcgtt tttcaaaaac agcccaaact tctttggcat
    36661 ctaagggaag tcctgtttct tttttatcta tcacagatga aaaaccatag tttccttcaa
    36721 taaagctaat caaggtatca taatattgaa gtcctgcatc ttttgcagtg tcgagaatga
    36781 gttttagctt ctttttatct acctttcctg gctcaaaaag ttcgttcatt tcgtcaaaaa
    36841 atgtctttga tgcaaaaact tctaaactct tgtagaggtc tcttaaaaac aactctgtca
    36901 aagcaacttg aatatccggc acgccctttc catcgaggtg atgatgcaag agtgcccatt
    36961 ttcctgtttc atcatctttc acttcacaga tattcaataa aacttgagaa gcaaagccgt
    37021 ttaaggaaac aaagaggcct ttttcatgca tttctttact ggagcgaata taccaaagcc
    37081 ctgtcttgag ttcttgaaaa atacaaaatg cctgcgctga aaagctcaaa cccaaggctg
    37141 aggcaaggct ttctgtttta agaattactt cttccccgtt tttttctgca tattgacaag
    37201 attcttttat ccaaccagat gcccgttcgt atttattatt ataaaagaca agacttcttt
    37261 cgtttttata tgcgtttgaa taggcataga tttcttcatt tacatgctct ccattccata
    37321 cgtcgtaaaa gcggaaattt tcgacctctg caaagagata gcgttttttt accaagggga
    37381 aaatttctct ttcatgtctt tccatgagat agagatcggg tttttcatcg atataggcct
    37441 ttgtatattc catgccgtat ttttcactat agccttctat ttgcccatgt ccaaacatag
    37501 gaagtcctgg catggtaacc atgagcgtac aaacaccaaa atatttgtca tctttaccaa
    37561 actgcgcgac agctgtctct tcatctggat tattcataaa gtttacaaaa cgtttcaaaa
    37621 cctgtggatc aaactcgaga gtatttttta ccgttgccct atacttaaaa ttttcttctt
    37681 gttttaacat attcataaag gccgaattgt atacgcggtg cataccgagg gttcgcacaa
    37741 agtagccttc catcatccaa aacgcttccg caagtaagag cgtatcagga acttcttggg
    37801 cacaacggtc tacaacctca cgccaaaact cattcggtat acgcgcatca aaatcagcat
    37861 gagagagggc atgttctgca cggcttgcta tatccccccc atgacctggc tctggatacc
    37921 ataatcggcg aatatgtttt tttgcaagca ccattgctgc atcaaagcga ataataggaa
    37981 aatttcttgc aacatgtaag attttttgta taacttcttc ccttgcttct ggattaagga
    38041 aatcaacctg cgctgtatcg ttccaaggta agcccgttcc atcatttccg tggtaaacat
    38101 agctcgttgc accacttgca ttgtcaactc gtttatagac aaccgcacaa tctgttttat
    38161 tataataatt atcctcgaga tagacggaaa tacgtgggtc aagtgaaaga ttttctccgt
    38221 taaaactata agtaggaaag gggcagtcct ttgtttgcaa gaaaagatgc ggtttttcta
    38281 ctacccattt tgaatccatt cccgtgtgat tcggcaccat atcacttgca agacgaattc
    38341 ccctatacca caatctcgtg cgcaaattcc caagcgcttc ccaaccacct aagccctctg
    38401 ctatatcgta atcatgcaaa ctataggcac ttgccgcagc ttcggggttg ccatttattt
    38461 gcttaatgcg tttactcgcc caagaacgct cccatatccc tataagccat aagccagtaa
    38521 aaccttgttg tgcaagcata tcgagctctt catctggtat ctgatcgagt gtcgtaatag
    38581 gtctttcgta tttttttgtc agctgatcga gccaaaccaa aacagtcttt gccatgagaa
    38641 caactcgtgg catccaatcc ttatcttgac taaagcgttc gtactcgtgt aacaagctat
    38701 tgtaatcata ggcttccatc ggaggcggtc ctgaaaaatg cccgctccaa ccaggtttat
    38761 cctcttcttg taaaaaatct attccagcta aaagacgcat gagccattcg ccgagtaaat
    38821 ccgcccaatg tgttcgaata taatcgagtt gtcccttcaa gcttaaggga ctaaactcca
    38881 ccggctcttt aaataattta ataagactcg tattatttgg cccaataaag ggctgctctt
    38941 caaaaaaatc ttttatttct tcccaagcct tgttatacga agcgagggat acaagttttt
    39001 tattagaaaa taaaaacaaa aaaggctcaa atgcaggatt ttcatttgcc agatggagca
    39061 agataaactc ctcgagcaca gtaattttat tgtcaatttc tgtttccgtc ttttgctttt
    39121 tttctcctgg aagtaaaata tcctttgcgc tcgtactccc tgaaagatat tcttctgccg
    39181 actgcttccc cttgtatacg ggaagcggag gaaattcttc aacaaaggca agcaataaat
    39241 cgttgagttt tttttcgccg agactttttt ctaaatgcag atataactca gcaaaggcat
    39301 tttcattttt ttctctacga aataaataac aaatataatg aaaaatttca tctatcaaag
    39361 ccatggcgtt tacatgactt gccttaagca ttttttgact ttcaataagc ggattaaaca
    39421 attcatttac tttttttgta aactcacgca cctctttaaa atgtgcaaaa ataatatttc
    39481 cacttgaagc aaataaaagc tcatcaaagg caattttttc tcttatctct cgcgaaatat
    39541 gaaattccat aagcggatta tttgctaatg tttcataaat tctcgtatct atagtatttt
    39601 gtttcattta aacctcgctg ctttttttat gtatacttac gatttcctta attttctgta
    39661 tcagatcttt atttttagct aaatcctcta taagtactgg taatttatag gtccagttaa
    39721 actcactcac tgttccagga atattgatac gctcatcttc acttttttcc gcataataaa
    39781 gcgcatctaa gtacaaaaag tcttgaatag gatttataaa ataattgctc ttcgttgtcg
    39841 ccataattga taataccttt tgacaaacta gaggagaaaa aagttcatga atataatcta
    39901 aggactgttt tccgttttca aaaaaacttc gcccaaagtc ctttgaatct ggctccttat
    39961 cccaccatcc tcgaagtgtc gatgaatcat gtacggagga agtgctcaca cttaaagctg
    40021 gatagtcagt aaagggaaca aagggcttgt cctctttttc ccagagtcga ttccacctga
    40081 caacacgcaa agcatttata gacaaggact ctagaacgcg gggcatcgat tgtaagttca
    40141 ccccaagatc ttcggcacag gcgatcatat tgccaactcc acttaaagct cccaaaagtt
    40201 ctcttgcctg tttttcccaa agcatatttt gtttttgctc tttttttgta aacagctctc
    40261 tcagcataga aatttgctca tcatttagtg ttttccaaga acttgtgttt tcataggtcc
    40321 ataaaggtag gtaggtgtct ttttttattt ctataagagc cctatcaagc catttttccg
    40381 caagtttttc ttttatctta tgaggaatat caagagacca aatatcggca tcggttttaa
    40441 tggattcttt aaaaagccat aactcttcgc taccgattct atccatacag gtagtaagta
    40501 tgccatgagt tcctagataa tctccattat ttacttcttc aatttcccgc gtcgcaacat
    40561 gcggtttaga aagccagcgt atagtctctt ttgaaagacc acattttaaa agttcctcgg
    40621 tactcataga agcgtttgga atagtccaac ccaatatcgc ctttgattcc ccttttggaa
    40681 cggcccaaat tctaaagaat ccaagtatat ggtctattcg atacatttga taatattttg
    40741 ccgcctctat aagcctttct ttccaccaag aataatcttg tttttttaac tcctcccaat
    40801 tataaatagg aaaaccccag ttttgtccaa agggattttc tccatctgga ggagagccag
    40861 cccttagatc ttcttgaaaa aattctggat aagcccatat gtcagcagaa tcttcattca
    40921 tgagtattgg aatatcgcct ttaagaagaa tatttttcgc cttgcaatac ttacttgctt
    40981 cttgcaattg ctcacttgca tttttttgta tccatacgtg aaagtataaa tcctcttttg
    41041 aaagaacgac atcccaaagc tctgcaattt cagcctttgt taannnnnnn nnnnnnnnnn
    41101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41161 nnnnnnnnnn nnnnnnnnnn nnnaatcctc ttttgaaaga acgacatccc aaagctctgc
    41221 aatttcagcc tttgttaagt ccgcatactc atcccaggtt ttaaatgaag cttccctatt
    41281 tttttccttc agtaccataa aagaggcata ggtctgtagc cagggattat catcgagcca
    41341 tttttttgta gactcagaag acataaaacc agcagaaatt tcttaccaga tccgttttaa
    41401 tagagaaagt ttaaaaaggc gcaatttttt gtaattaaaa cgtttttctt tcgtaaactc
    41461 tcgttttttt tcttctattt catttaaaat tgcttttgaa agattcgatg aaagcaagga
    41521 aaactctttt atctttgaaa cacgaatata tatcgggtga agtgcaaaag ccgaaagtgc
    41581 actataggga gaactttctg tgcccgtatc attaacaggt aaaatttgta taaggcttaa
    41641 gcctgaattt agacaaaaat ctgcgaaagg tatgaggtct aaaaactccc cgcaaccgag
    41701 gctttctttt gtttgtaagg ccgaaatagg ggcagcgaca cctgtgcctc gtatatcttt
    41761 tatttgattt gttaattttg cattcatatc gtttaagccc tttgtcattt cttatagtat
    41821 accataatgc atgctattat acaaataaaa ttaaaattca ttgcacattt aaacaaaaaa
    41881 aagccggcct cttgccggct gttttagatg ttaaaaatct aaacatctca taaaatgtct
    41941 ctcgtaagag agtaaaaagg tttctatgct ttgctccctt cggagctata ctcttttggt
    42001 ggtttttcca tgaaggcctc ctcagttttc tacaatacat cagaagtaat ctgaacctta
    42061 aagagctatg atttaaggaa aaccaatcta ggaccttggt tacttcctta tctcttctgc
    42121 tataatgata gcatagattt aaaaaaacgc aagtcaaaaa tcttataaaa gtaatgtaat
    42181 cacaaaaaaa ggactacccg tttggatagc ccttttctta aatcatacgt tattagtaaa
    42241 cgtaggtttt atacagctta tttgtttaaa gcagcttgta ctttagcagc aatttcagct
    42301 gcagttgctt taccagctac gatttgttgc ataccagcat tgagaaggtc atttggatca
    42361 ccttggatgt aagagtcgat aacttgtgct gatgggtatc cagctgcacc gtaagcaact
    42421 ttttcattga tgattgcagg catatcgtta ggaatagcag cttttgttac aggagcaaca
    42481 attgcaccgt ctcgaagtct ttggattact tctgcatcat tgttgaagta gtctaagaat
    42541 ttaactgcag cttctacaac accatcttca acagcttttt ttgtgaatcc ataacctaca
    42601 gaaattgcac cagctgagct aagagctgca gaagcttttt cgccaggaat ggttggaaaa
    42661 gacataagtt ttgtgtcttt tgcgatttca ggaacgattc caccagcttc ccattgtccg
    42721 cctaagagga aaagagcttt tttggttgaa aagtcagaaa cagcaccttg tgtgtcaact
    42781 aatactgcat ttgatgtgat aacaccgtct ttaaccattg ttgttaagaa atctaaagct
    42841 gcaactacgt ctgcatctgt aactttcttt ttaccagcta gaatttgttc tggggcatca
    42901 agtaaaccag ttgttcgact gatgattgct gacattacgc atgaacccca tacccagtct
    42961 gctgctccac caactgcaag tacttgaata ccttttgctt tagcgacagg aaccatagct
    43021 accatgtcag cataagtttt tggcattgca agaccaagtt cgtccaataa tgctttgtta
    43081 gcaaaaagaa ctgtacagtc attggttgtt ccaagaggaa cgtactcgcg ttttccatct
    43141 tggagaggga atgcatcaat attgtaatgt gctttgttaa gatatggtgt atgatcaact
    43201 aattgaccag cttctcgcca tgatccgccc catcgagcat ctgatgagaa ataagcaacg
    43261 tgtggaatat caccacttgc aattcgagct tttgcttttg agtgatatgc atcatcataa
    43321 agaagttcga aatctacttt aatgtctgga tttgcttttt caaaagctgt tacaatacga
    43381 acaaagtttt gaccttcagc attggagtta tctccatatg ctaaaatttt taatactgtt
    43441 tgacttgact tagtagtttt actacaacca ataaagccga gcccaattgc taaaacagca
    43501 aatagactaa ctaaaataat tctttttgaa agtttcatct actttcctcc ttaaaaatac
    43561 tatatgctta aaatcgcatt tctgcgatgc acagcccaaa attgtatgat ataatattac
    43621 cagcaaagtt caattttgta aagctttttt acgtgaaaat agtatttttt tatgtgaaaa
    43681 caacgttttc tatttgttct aaaaatctaa taccacaact tcaaccgaaa ttcttattgt
    43741 aacgaaaaaa agtactacaa tcaatataat ctaaacattg ctctcttttt ttttttttac
    43801 tatactggtt actatgaatg atgcaaaaaa gaaatctgga attgtaacaa ttattgggcg
    43861 accctctgtt ggaaaatcta ccttccttaa taccttaagc ggagaaaaaa tctccatcgt
    43921 ttcacctatt ccgcaaacaa cgagaaatgc aatccgcggc attatcaata cctcgcttgg
    43981 gcagttggtc tttatcgata cacctggcta tcatgcttct gaaagaaaaa taaaccaatc
    44041 cttcaaggcg ctttcaaaag aacaaagaga ttcaagcgat gcgatagtat acatgcttga
    44101 tgcaagcagg gaaccagggg aagaggaagt cctaacaagc caacttatag caccttatat
    44161 agaaaaaact attattgtta tcaataaaat agatttagca aagctcgatg aaaagaaaaa
    44221 ttgccttgca gaaaacaaag cgtatataaa gaaaaccttc cctcagtttg acgaaaagaa
    44281 tattatagaa gtttccgctc aagatgatac aaacttgaac gaagtcttaa aagccttgta
    44341 tgaaattgtc ccagagggag aggcgctcta tcctgaagag ttttacacgg accaagaagt
    44401 tgacttccgc atagcagaaa ttgtacgtga aaaagcaata gaaatgttta aacaagagct
    44461 gccacacgca atctttgttt ctatttccga tatggaatgg aaaaaacctg gcaaggaact
    44521 ttgggtgcgt gcctttttaa atgtcgaaaa agaaagccaa aaggggatgg ttataggcaa
    44581 gggtgcaact atgataaaaa ccattcgcgt tgagtcgatt aaagctctac gaaagatttt
    44641 ttcctataga atagacctcg acttacaagt tcgtgtaaat aaaaactggc gacaaaaaga
    44701 tcatatctta aaaaagatta tttattagtt tcaattgtat agataataga ttatcgatat
    44761 aaaaataaaa tgtctaaaaa ctattactga ggctgatttt tatcgagttt ctacacttcc
    44821 gtcagccttt gctataaaaa caagggcttt ctttttggta aaaaaaccgt tatctaccac
    44881 accagttatt tcgtttatct tcgtttcata gtcgcttgca gaaaagaggc tttctttatc
    44941 ccaaagtgca tcgaggataa agtttccatt atctgttacc acgggtcctg cttttcgtag
    45001 tccttctcgt attgcaacct ttgcgccaag cgcctttaag cctcgctcta cgatgagccg
    45061 tgattcagga agaatttcaa caggcagcgg aaaggcacga gctaagcttt caacagtctt
    45121 cgtttcatca gcgatgataa caaagtgttt tgacgcatag gcgatgagtt tttctaaaaa
    45181 aagtgctgcc ccgccaccct ttaccagctt attttcaatg tcaatttcat cagcgccatc
    45241 aatcgcaaga tctaagcttg catcgatttg ctttgcattt aaactaaaaa ctgaaaagcc
    45301 gagattttca caagcaattt gcgtttgaaa actcgtcggc actaccttta tatctgaaag
    45361 aattcctgag gagatttttt tggcaagata cgtaatcgca ggcataaccg tagaacctgt
    45421 tcctagcccc agtttcattc ctgaaaaaag caaacccttt tctataagcc tatctatagc
    45481 gtattccgca actctttgtt tttgttcact ttgacttagg cttttattca tatttctctc
    45541 ccgtaaacaa tgaaaactgt gcctcagctt ggtattcaag catttttttt ccgttttcaa
    45601 ttctacaccc tgctttttct gcacgtagta aaagcggggt tttttctggg ctatacacca
    45661 tatcgtagac gagttcgcgt cccacaaagg tataaaacga aatagggtca tgttcttctt
    45721 tttgcatttc ctcttggctg actcctaaac caactgaagt tgtttgtata atgaggtcag
    45781 aataatgttc taaaatatca agagattcat aatcaagcga tgcccaccta aaattatagt
    45841 attcggccaa agagcgtgct ttaaaaatag ttcgattaaa aatacaagcc ttgccacgta
    45901 aattatgcac aacatgggct gcagcacggg ctgcacctcc tgctcctaaa atggcaacct
    45961 tcatacgaga aagatttttt gtaccaacaa attcaagaag tgatttggaa aagccataaa
    46021 catcagtatt atatcccttc cattctccat cttttttaac tatcgtattg caagcgccta
    46081 tttctcccgt tacctctgtc atagactgca aatgatctat aaccttttct ttatgtggaa
    46141 tcgtaacaga aagccctgaa ataccaagca catctgcaaa ctcgattgct tcttctattt
    46201 tctctgcttt caaaggaaga taacttgcat taataccatg gtttttatag ccttgattgt
    46261 gaattttcgg actacctgtt gttgtgagtg gatacccagt tataccaaaa agagcagttg
    46321 catcgtcaat tgttttgtaa ttatatactt cctgaagtgt aatcggatct atgtgtccaa
    46381 tatcataata attaaagagc gtttcctgtg gagaagtata cgaaagtaaa gaaccaaaac
    46441 gttgtgcaag aacacggctt gcaagcccat aaggtcccat tgcaagtaca atatgatcgc
    46501 ccttgttttg tttttttacc tcttgcactt gtctatataa atttgttacg tcgcttaaag
    46561 aatgagccat aacggcaacc ttagggattt catagccagt catgcgcaat gtttccattt
    46621 tttctgcaag attatcgatt gtatgatgca tattgtggta gctgcggata atctttgtgc
    46681 caaaagcaag tgccgcatct tgtaaacaag gaacatggaa gtcttcttct aggtcgatat
    46741 aggcaaaatt cttgcttgca tcttgatccg caaaggcaag tcccctcgca aacagagttg
    46801 tacgagcagc ctctccttcg gtaaattttc ccccatcgat gcgacggcga atggttaaaa
    46861 tacagggaag ccccgcaagc tctggaaaac gacgaatata gagccgttca tcctttgtta
    46921 gataatctac ccgtaactct accatatcta tccaattacg atatttttct atcacgagta
    46981 aattttcttt tatagtcgaa caactgagac aaagacaaat ctttgaacca ctcattttta
    47041 aatcctcaca aaacaaaaat catagtttgt ccaagtgtat aaaaacaaac taatcaacta
    47101 aaaaacgtgc tgcttctttg ttggaaatag agagcacaag ttcagtcccc cttgcaacag
    47161 cgtatggaat agaaacattt ccaccgaggt gataaaactg cacaagcgca tatcgatcgc
    47221 cctctggaag aattgcttca agctttaact ctactgcata tgggaattct tgcaaggttt
    47281 ccttaaacac gccgtatatt aagccatttt cttccatatc ttcagggaag gcaaaaacaa
    47341 cagatacacg cgagtaggca tttacatagg tatctccaac aggtagctga gacacaaccc
    47401 gaccaatacc ttcatctagc tctggcgtcc tgaaagaaaa atcatacaac aacttagttc
    47461 tattcataac gagcaaggta tcattgagtg aaagattttc aagactaggg attttaacct
    47521 tgtcatactg agaaccctta ctaagcccta ctttcaaaac aattgggtcg ctaatcttgg
    47581 tgtctggagc tggatcctgc cataaaatag taccctcctc ctgttcatta aactcataag
    47641 aaaaattctc atccaataca ataagtggtc gagtatagcc agtaaaaagc gattgcaaat
    47701 cgagttttac actatctatt ttttgcccaa cataatcttt tactgtatct ataaccgccc
    47761 cacgactgac aacgagattt atgcgtcggc ctgcctttac gatagaaccc gctggcggac
    47821 tttgctctaa aaccgtcccc ttgtcatcga caaaattaga atacttcatt tgcaattttg
    47881 gatagagctc cttaacctgc atctctaata aggctgctga aagttccttt cccacaacat
    47941 ttggcaccat tacctcttct tttcctttaa ccgaaagaaa aaataccgcc attgtaatta
    48001 ccgccataac cataatagtt aagagggaca taagaataag cgcttttcca tttgcttcaa
    48061 gagattctaa aaaatctgca aaatcttccc gtaacctgtg tattttcata cctttccttc
    48121 tattgtactg caaacagcac ctatgaaatc ttttgtgccg ttcaaaaaat ctttccaata
    48181 catggctttt tttgtatgcc attgcaattg ttcaactgct agaattccct ctccggtttg
    48241 aataaggatt cccgtcttgt cgcgagctaa aacacttccc gctttttttg tttcgccctc
    48301 tgccgtattc gaatcactaa agaccgaagc cttgagaatt ttcaacattt ttccttcaac
    48361 ataggtaaag cttcctggcc aaggtgtaaa agcccgaatt tttgcatcga tatcgagggc
    48421 agaacaattc caatcaataa gtccatctgc cttacaaaaa agcgaactat aacaagctga
    48481 cgacgctttt tgcttttgct tttttatcga atcgttttca attgcatcca aaacaggaga
    48541 aatgagtttt gcagaaatat gggctgcatc agctaacaag ctttcactcg tttcagttcc
    48601 atcaaggtct atcgtctctt gagcgagaat atcgccacaa tccatttcta gagctatttc
    48661 ttgaatcgac acgcctgttt gactatccct gttcaatata gcttctggaa caggggcgca
    48721 acctcggtat tttggcaata aagacggatg aatatttata ccgcctttcg ggaaaagcga
    48781 taaaaacttt ggtccaaaaa ttttcccata ggcaaagcaa acaagcaagt cgggttttaa
    48841 agctaaaacc gcctggcgac atgcctcatc aagccgctct ggcgcaagaa ctggtatatg
    48901 agcgtattca gcatgatcta aaacaaattg cgcaagtggg cttaaaataa gctttttact
    48961 acgcccctga gctgacggtg gatttgtcaa aagacctaca acttcgtggt ttgcttcttt
    49021 taagatctgc aaaagaggaa taattgaaat atctggactt cctgcataga gaattttcac
    49081 ctattgcttt ccttcgcagc ttcgagcttt gctttttttc gcgctttttc tttttcccgt
    49141 tcagcttttt tttcttctct tcgcgcgatt gttttttccg cttcttcttt taattccggg
    49201 tctaagcgat ctataaataa aacaccttct aaatggtcgt attcatgctg tataatgcga
    49261 gcgagatagc cctccgcttc gagagtaaaa ggctttccgt gttcatcgag agcttgcact
    49321 gttatttttt caggacgctc tacctcggca tagatttgag gaacagaaag acaaccctcc
    49381 tcaaaagagc aggtttcttg tgaagtcgct atgatatgcg gattgataaa aacacgatct
    49441 ttatcgtcat ctgagcgcag aacaaataaa cgctgtgaaa gtcctatttg tggtgcagca
    49501 agtccaacgc cctttgcctc atccatcgta acgaacatag cttttacgag ctgacgagtt
    49561 tcatcattta tgtcgacaag agcttctgcc ctttggcgaa gtacttcatc gcccaaaata
    49621 cgtaatttca tatctaaaga atagtataaa agaagatgaa attcaagcat agcttttagg
    49681 ttcttgacaa aaagccgaat aaagggcatg aattaggtat gcaagtttta caaaacaata
    49741 atcccgaact catatcccaa tttattgaca ctcatattgt tgaagctttg gattcattta
    49801 taaaggatga atctatcgat gccataacaa aggccgcgat aaccattgca ccttgcttcg
    49861 gagtagagaa agaattttgc accttgatag aaggagagac cgaggtcagg gcaaatgccg
    49921 aaaaaaaact cactacaagc ttttacaaca atcttttact cttagttcaa aaaacctggg
    49981 tagaagcctc cgatgaagca ctcaaaaacc aagccctcga aaaactaaaa gactgttgtg
    50041 gaaaagatca tcatatcgac tataaagcct catacgaagc ttttatagat atgctccacg
    50101 atgtagtcta cctcatgttt ggaccacaaa caaaaaaagc tgatttttca gaatatgccc
    50161 tacggataga cccaggcttt ggagtctttt ggtggtattt acaagctctt ccaaaaaaaa
    50221 cagattggtc tttagacaaa acaaggttag ccctactcct tggaatgttt ttcttagcta
    50281 attattaaaa ctcataagat aaaataggta tcgatgatga attttgaaca agtctgccaa
    50341 caattgcgag tcggcgcaaa caagctctca caagtcaatg caggtgaaaa aaatgcagcg
    50401 cttcaagcgg tagctatagc cttggacaaa aatcgctcta aaatactcga agcaaataaa
    50461 aaagacattg aaactgctcg tgcttctggc atgaaagaaa gcctcatcga cagactcatg
    50521 ctcgatgata agcgaatttc aagtattatt gaaagtatcg ctattattat caatcaaacc
    50581 gacccaatcg gtgtcgttga aacaggctgg acaagccctg aaggcttaaa aatccgaaaa
    50641 gttcgcgttc ccatcggcat tgcggcaatt atctatgaat cacgaccaaa tgttacggtt
    50701 gatgcctttg ctattgccta taaaagtggc aatgctatct atttacgtgg atcatcctct
    50761 gcccttaaat ccaacaaggc gattgtcgcc gcgataaaag aaggtttagc agaagcaaaa
    50821 tctggcgttt tagaagctat cgcccttgct gaaactggtg agcgcagtga agtcgatgaa
    50881 atcctcaatg cggttggaaa aatcgatgta gttctccccc gtggaggcgc tgctctcata
    50941 aaacttgttg tagaaaaagc acgtattccc gttattgaaa cagggagcgg tatttgccat
    51001 ctctttgtag ataaaagtgc tgacctcgag caagctgtcg aaatagcctg caacgcaaag
    51061 ctacaaagac caggtgtttg taatgcaatc gaagtcttac ttgtgcacga agccataaaa
    51121 gaaaactttt tggaaaaact gggaaaaaca atgaagggaa agctactttt caaagcctgc
    51181 cccgaaagtt atgccgtttt aaacagaata gaaggtctac aagtagaaaa agctggcgat
    51241 acagactttg acacagagtt tttggacaaa attcttgcgg ttaaagttgt ttctacactc
    51301 gatgaagcaa ttgaccatat aaacacgcac aatacaaagc attctgattc tatactgaca
    51361 aaaaatatta gccatgcaca aaaatttcaa aaccaagttg actcatcctg tgtctatgta
    51421 aacgcttcaa cacgatttac cgatggcgga gtctttggct ttggtgctga actcggcata
    51481 agcacacaaa aattccacgt acgcggtccc atggggcttt cggctcttac cacagaaaaa
    51541 tatctcatcg acggcgaagg acatattcga taatgagcga aacaatagat acatacaacg
    51601 cagggaaaaa ttcttgtccc acaatacaag aaattctcaa aacagctcga aaaattgtta
    51661 taaagatagg ttcaaacacg cttgcaacaa gcgacggaaa aataaacatc cctttcatgg
    51721 aatcatttgc cctacaatgt gcatccctca tgaaaaaagg aaaacaaatc gttattgtct
    51781 catctggcgc gcaagttgca ggactttcta ctatcgatcg atgggagaga aaaaaagaca
    51841 tacactatcg acaggctttg tgcgcaatcg ggcaagttga acttatggac aactggcgaa
    51901 atgccttttg gaaaaacaat attcatatag gacaattatt gctcacaaaa gatgactttc
    51961 acgatgacaa tagaaccctc aatatgcgaa acacactttt tacccttgtc gatgaaggcg
    52021 ttatacctat tatcaatgaa aatgactctg tttccactga agaaattcgc attggtgata
    52081 acgacaattt aagcgccctc accgcaattt tgtggaatgc tgaccttctc gttctgttta
    52141 gcgacataga tggtgtctac acggataatc caaaaacaaa tcccgaagca aaactcatag
    52201 aatatgtgcc gagcatggac gatttgcgtg caaatattcg ccttgggagc gcaaactcct
    52261 ttggtaccgg cggtatgaac acaaaacttg aagctgcaga caaactcact tcctacggta
    52321 ttcccatgat acttgcaaat ggctcccatg aaaaaatctt ggaaaaactt gcagacggta
    52381 gccaaaaagc aacgcttttt tgtgcagaat aaacagagcc tagaaaaaca gctagaaatt
    52441 gccgatacta tagcaaagcc tccttttttt accaaagagg gcgtatacaa aaaaatctaa
    52501 ggaaaacatg atgaataaaa taggaatact cggttgtggt gtaatgggtg aagccctagc
    52561 gaaggcaatg ctaaaaaaaa cagaaggcaa aaatattttt ctatttgata tagatacaga
    52621 gcgcacaaaa aagcttgccg aaaaactctc ttgtaaaaca gccccgtcaa gtagcgactt
    52681 ggtaaaaacc gtcgattttc ttttattggc tataaagcct attcatgtaa aaaatctcct
    52741 cacagagatc gcctcagaat actatcaaaa aaaacctgtt ttaatttcga tagttgcagg
    52801 tatacaaata gaaagtctcg aaaacatgct ctattctacc ttgcccttaa agacagaaga
    52861 aaaaaaagta aaaacagcct ttcccattat tcggcttatg ccaaacgtaa gcgttcttgt
    52921 cgaagaagga atgatagctt ttagcagcaa tgatgcggta caggcaaaag aaaaagactt
    52981 tgttatcgat ttgcttgaaa aaacgggaaa aatagagatt gtccccgaaa atcttatgga
    53041 tgcagttaca gctatttctg gcagtggacc agcctacgga tttcttttta tagaagcgct
    53101 tgcagatgct gcagtgctct tgggtatacc gagaaaacaa gcctatatct atgcagcaca
    53161 aacacttaaa ggcgccgctg aaatggtact cgaaacgggt gagcaaccag gggtgctaaa
    53221 agactcagtt tgttcacccg gtggaacaac gatagaggca gtaaaaaaac ttgaagaaaa
    53281 aggctttcgt tcagctatta tggaagctgc tgaagctgcc tttaaaaaaa gctgttctct
    53341 agggaaaaaa taattttaaa ataaacagcc ctgttccatt gaaaaaagcc taaatttaca
    53401 ctataattcg gtatcatgta tagaatttat gtagaaaaga aaacgggctt ccaagttgaa
    53461 gcacaaaaag tagagcagga gcttaaagat tttttaggca tcaatggcct aaaatcatgt
    53521 cgtttcctca ttcgctacga cattgaaaac cttcccctcg ctcttagctc gaaggctata
    53581 gacgaagtat ttgtcacaga gcaaaccgat agggtgttta tcaatcatat tccacttgca
    53641 gaagatgcta ttgttattgc ttgggaattt caccatggcc aatacgacca aagagctctt
    53701 tctgcgcaag aggctttaaa tcttctactt gtaaaacata aggcgacagc acaagatgat
    53761 acaaaaaaag ccattatcgt aaaacctgca aaaatgcttg ttttacaagg cgatataaat
    53821 gcttctgata taaaaaaaat agaatcttat tttataaatg aggttgattc tcaaaaagca
    53881 aaccttgata ttcctaaaac gctttcaaaa gctatccctg aaccaaaaac aattcctttc
    53941 taccaagatt ttatcaaact cgatgaaaaa ggcttgctcg cttttcgaga agaacagggt
    54001 tttgcaatgg acaatgcaga cttagttttt gtgcaagact atttcaaaaa agaaggtcgc
    54061 gatccaagcg agacagaaat gcgtgtttta gatacctatt ggtcagatca ctgtcgacat
    54121 acaactttta acacaatcct cgacgatata gaaatagaag agggctattt taccgactta
    54181 tttaaaaaat cactcgaatc ctatactaca ttacgaacgg aaatacatac aacaaaagaa
    54241 aaacccataa cgctcatgga tatggctacg attggagcaa aatatctaaa aaaagagggc
    54301 aagttagacg acttagaaga gtctgaagag ataaacgctt gttctgtttt tatagatgtg
    54361 catactgaaa atgccgaaac caaggaaaag caaacagaaa aatggcttct tatgttcaaa
    54421 aatgaaaccc acaaccatcc aacggaaata gaaccctttg gcggggcaaa tacttgtgtc
    54481 ggaggaggca ttcgtgatcc actttctgga cgttcatggg tctatcaggg tataagaata
    54541 acaggctctg caaacccacg cagaaaactg gaaaaaacac gcgaaggcaa actccctcag
    54601 agaaaaatta caaaggatgc cactgccggc tttagtacct atagcaatca aattggttta
    54661 aatgcttcgc aagcggtaga aatatatcat cctggctatg aggcaaaacg aatggagcta
    54721 ggagctctta tcgcagcagc gccagcagag caggtaaaac gaggtatacc acaggccgga
    54781 gaccttgttg tccttctcgg tgcaaaaaca gggcgtgacg gcataggagg agcgacgagt
    54841 tcatcccgtg tacaaaccgc tgaatctgtc gaaacagcct ctgctgaagt acaaaaaggc
    54901 gacccactag aagagcgaaa aatattacgt ctttttagaa attctaaagt tagtagcatc
    54961 ataaaacgat gcaatgattt tggtgccggt ggggtttctg ttgctgttgg agaactcgct
    55021 ccagggctgc atattgacct cgacgccgtc cctacaaaat acgaaggctt aaacggcaca
    55081 gagctggcta tttcagagtc gcaagaacgc atggctgttg ttatagacgc aaaggattta
    55141 gatttcctca tcgatgaggc agacaaagaa aatctcaagg ggacagttat cgcgaaggta
    55201 acagatacaa atcgtcttgt tatgcagtgg aaggaacaaa caatagtcga tttggatcga
    55261 aacttcctcg acaccgctgg tgccaagcgt catgcaaaag caaaaattgc tgaaccaaat
    55321 cgtatttatt cgcctcttgc tcgccctctc gacgcggtac aattagtttt aaatgacgaa
    55381 agtcggggac gttgcaaaaa agaaacgctc aaggatgcct ggttcacaaa tatgagcagc
    55441 ctcgcttgtt gctcgcagca aggaatggct gaacaatttg atgcgtctat cggggcaaac
    55501 tccctgctct ttcctatggg tggaaaaaca caaagcacgc cagaagtagc ctccgttgca
    55561 aaaattcccg tattaagtcc aaaagaaaca agcacaacca gtatcatggc ctatggattc
    55621 gacccgctcg tcgcacagtg gagtccctgg catggtgccc aagttgcggt gctagaatct
    55681 ttggcaaaaa tagtttctgt tggaggaaat ttagaaacaa cccgcttaag ctttcaagaa
    55741 tattttgaac gaactgactc gccagaaacc tggggaaaac ccacagctgc tcttcttggt
    55801 gccctcgatg cacaaaaagc gatggaaatt cctagcatcg gcgggaaaga tagtatgtca
    55861 ggcaattttg atgacttaaa agtacctcca gccttaatat cctttgcctt cagctcttct
    55921 gatgaagaaa acctcatttc tggcgctttt aaacagggcg attcctacgt atatgctgca
    55981 attcaagcct ataccgaaat gcttgcaccc gattttgacg ctttcaaaga aaattatgcg
    56041 catattaaaa gtgcacttaa agaagaaaat atactttcaa tgtatcctgt aggaacaggt
    56101 ggtatttctg aagcaatttc aaaaatgagc tttggtaacg agataggttg taccatagaa
    56161 agcggcgtgc aagaattact ttttgtgccc atgtacggca cgataatcgt tgaaacaaaa
    56221 gaaaaaatag acacgtcaaa ggtacgctgg ctctatattg ggcggacaca agaaaagccc
    56281 gttcttagcc tcggcttaca ttgctatgac ggtggcaaga aaaataaaac aaaggaaacg
    56341 gagcttcaaa atattgaaat ttcaataaaa gagctaaaag aagcatggaa aaaccctctt
    56401 tctgctgttt tccctcttga atccacagaa aaaatccaaa aaatacctgg ttttgcaaaa
    56461 aaacgacatt cgtccctcaa aaagcatgat aaagctttaa aaaataaaaa tttttatcat
    56521 ataaaagaaa aaaaaccact tgttttactt ccggttttcc ctggaaccaa cggcgaatac
    56581 gacatggaaa gagcctttac tctcgctgga gcgaaaacca aaacgcttat tttcagaaat
    56641 aagaccgagg aatctacaat tgaatccttt gaagctttaa aaaaagctat ccttgaagct
    56701 gaaattcttg cattttctgg cggttttagt cttggtggcg aaccagacgg acttggtaaa
    56761 tacaccgcat caatactcca gtatcccctt ataaaagatg ctattatgca gtttttgtca
    56821 aaagatcgcc tcatacttgg ggtcggagat ggctttcacg cacttttacg ctcaggactc
    56881 attccctttg gcaagattaa agaaaatact accggaccaa tactgagtgc aaacagaata
    56941 gggaaacata tttctcgaat gcttgatact aaaattatct cagctatttc tccgtgggca
    57001 ccaaaagaat gcttaaaaga cagcatctat cgtttgcctt tttcaagtac agatggacgt
    57061 cttgttcttt caagggaagt tgcaaagcag ttgttcaaaa acggtcaagt gttttcgcaa
    57121 tacgtagatg cagatggcat aactgcaaaa acggaaacca aaaatccaag tgcatctttt
    57181 tatgcaattg aaggtcttac cgacccaagt ggaaaaatac tggggaaaat ggcacataat
    57241 gaacgaagta tttcttttaa agaaataaaa aatgcaagtg aactctttaa aaatatccct
    57301 ggaaacaaaa gtcaagatat ttttagtgcg ggtgtatctt tttttaatta agttcgttat
    57361 tatagtagag atagaagaaa cagtgctttt tgaataaaag gcactgttca tttattttaa
    57421 atttatataa aagtcaaatc cgaaaaactg cttttctcat tgacttttct taggagcagt
    57481 aatgaaatca attacattta aatcaattag caagttgatc ctcggtcttc ttgtaatcct
    57541 cgtgctaatc gcttgttcga gtacccctac agaagcacac tggttgtatg acttcgaaga
    57601 agcacaaggg cgtgctgaac aagaaagcaa aaacattctc ttgttttttg ttggatcaga
    57661 ctgggatgag gtaagcatag cactttcgaa tgatattttc ccttcaaaat catttctatc
    57721 aaaaattgcc tcagaatata tcttagtaca actcgacttc ccacaagatg aaaatctcat
    57781 cacagaagaa cagttttata gaaattatga tgtggcaaat cgtttttctc ttgaaggtat
    57841 tccgtcagcc gttctcacaa ccaaagaagg ctatattata gataaatata atggaatagc
    57901 tgatgatgaa ataagtgatg ccgaaaaagc cgcagagttt ataaagcaaa tccaaaaatc
    57961 caagaaaaaa tcaacaacta ttatctccac ggtaaaaaaa atggataaaa ccgaaggtcc
    58021 aaaaaaagca aaacttgtag acacaatctt aaaccaactg aaccccgact atacctatct
    58081 tcacacagaa ttgattgcac aaattccaga acacgatcca gataatacaa caaagttgca
    58141 tgaaaaatac ttgttgcaac tcacctaccc aaaggctatg gaagcgctgt acacaggtga
    58201 ctatgaaaac gcaatacaaa tgcttgttga agttgcagaa aacaaaaaaa tctctaaaaa
    58261 agattctcaa gaagcatact atactgcagc ctacctaaac gaacaaatga atttaggaaa
    58321 agagcaaaca cttatctatt tacagctcgc ccatgatgta gatccagaat ccgaaattgc
    58381 gcaaaacgtg caacaaatta tttcaatgat tcagcttgaa atggaagagg aagaagcttt
    58441 ctactactca gaagaatacc gaaccgaggg caatgcaaac taattccctc tcttcacttt
    58501 ggtttatagg actcttgttt ttttcaggag tcctcatttt tctttcgatg gttttttcag
    58561 catctgagtc tgctttttta tctataaata ccttacgttt acgcttactt aaagataaaa
    58621 agcacaaaca agcgatgaga agctcaaaac ttctagaaaa aaaagaactt ttactaaaca
    58681 caattcttat agcaaataat attgtaaata taagcctttc tacagtgata actgcaatct
    58741 gtttagaact ttttggtaat gccgccataa gtatcgcaac aggaattgtt accttggtcc
    58801 ttcttgtttt tggggaaata agccctaaga ctatcggcac gcatcaccct gaaacaatag
    58861 catttttatt ttctggtttt attcaatttt tctcccttat cttaaaaccc tttgtaatat
    58921 tttttagctt tttttcaaac ctttttttaa aactcctcgg tatcaaaaaa aaagataaaa
    58981 caaaaagctt tagtgaagaa gaaatcatac actttataga aataggtgaa gaagaaggcg
    59041 ttgttgaagc aattgaaaaa aaaatgctcc accgtgtttt taagtttacc gaccttgatg
    59101 caaaaaatat catggtacca cgaatgcaaa ttacctttgt aaaatctgat gccagctaca
    59161 cgagcataat ggagctttca caaaaaacac atctttcaaa attccctgta atacaagaca
    59221 ccatcgatac tgttctcggc atactgcatg taaaagatat gctacctttt atttgtgaca
    59281 aaaaatcttt tgcagtaaaa aaaataatgc gagagccgct ttacatcctc gagacaacac
    59341 gcatgtcgca aatccaagaa ctattccggc aacacaatca aagcattgca atagttctcg
    59401 atgaatattc tggaacaagt ggtttggtaa caaaagaaga ttttacccaa gaagtttttg
    59461 ggactatgta tgatgaatac gacagttcag agatccccga aattcaaaaa ctttcacagg
    59521 agcatttttt agtatctgga aactcaagac tctttgatat aaataaaaaa acaggactca
    59581 atttaagctc agactactat gaaacacttg ctggttttat acatgagcag ctcgacgact
    59641 taccagaaga aggacaagaa ttattttttg aggactatcg tcttagaatt gaaaaaaaag
    59701 aaaaacaaaa aatccttctc gtttcgcttt ttatacaagt gaatgagtag gtagggactt
    59761 aggagaaaaa tggtttttat cattatacta ttactttttt tta
//
LOCUS       DAOSSW010000002        53851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_682_length_53851_cov_6.770113, whole genome shotgun sequence.
ACCESSION   DAOSSW010000002 DAOSSW010000000
VERSION     DAOSSW010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 53851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53851
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_682_length_53851_cov_6.770113"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="PKW26_00255"
     CDS             <1..1425
                     /locus_tag="PKW26_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOQ92028.1"
                     /translation="AYTSLIFDEAHGIEDSATSFFSSSLNRFTVLKQLSLLARSRKNA
                     HTGLLFSLDVLSNSPEYLQECLVKMQDIKTALTHFETEALELMEKDYAFRFTAGTAFR
                     LRSFLEAVADLHRAFHSFFTSFRALIQGIDEEDKSLPAVWEGKQIYKRLEYIAEVCES
                     FLDWDQQEDYVFWMEKRRLSFAQNKNELFSKEDGQSYPVIHKTPLSIAKTMNTGVFSQ
                     FETVICTSATLQTSSGFSFWLQRNGGQYLSKERLICAAFPSNFNYRENAVLAIPTDMP
                     LPDSPLFQASVEKNVLKLLQASEGRALVLFTSFESLRSTASYLRDNSHLLIYPLLVQG
                     EADRFNLLKQFKEKKDSILLATSSFWEGVDVPGDSLSHVIIVKLPFAVPNEPVFAARS
                     EAIEKQGRSSFAELSLPGAIIAFRQGFGRLMRRNTDRGIITVLDRRLLAKSYGRLFLA
                     SLPETRQLFSPMEDLIFEIKSFLK"
     gene            complement(1522..1692)
                     /locus_tag="PKW26_00260"
     CDS             complement(1522..1692)
                     /locus_tag="PKW26_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92029.1"
                     /translation="MAGASKNSRKSVATQKFMCSCGGEVSMKTLFDVGKIKNIAECQK
                     CKRVERRPKDFN"
     gene            1829..2113
                     /gene="rpsT"
                     /locus_tag="PKW26_00265"
     CDS             1829..2113
                     /gene="rpsT"
                     /locus_tag="PKW26_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOQ92030.1"
                     /translation="MAVKNASAKKRHKQSEDRRLRNKAVKSATRTSARKFVEAVHVKD
                     QALASEKLGILLSELDKAGKKGVIKPNTVSRKKSRMMKLYNASFETASAK"
     gene            2177..2488
                     /locus_tag="PKW26_00270"
     CDS             2177..2488
                     /locus_tag="PKW26_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOQ92031.1"
                     /translation="MQSKKVTKYDLVEAIYEDTNYEKKIIQDIVESFLEQVKESLVQS
                     KTIELRGFGTFELRLRKGKAAARNPKTGEQVSVAPHYVAAFRSGRDLKNSLWAIPTPK
                     N"
     gene            2461..4197
                     /gene="lnt"
                     /locus_tag="PKW26_00275"
     CDS             2461..4197
                     /gene="lnt"
                     /locus_tag="PKW26_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOQ92032.1"
                     /translation="MGNTNTKKLMQNKTKTMLGLQRFFIHFALLLSSALLFSLSHPNS
                     IGQKGFFFLAYFMYLPLLILISRLRLKESFFYGFLYGIIAYCIFTYWLATFHPMGILV
                     ISFLYGFQYALLFPVLVSARIILERQKLTSLVFAKKLLYMQWLILSAYEFIKTLGFAG
                     FHYGLAAYTQWSLPPIIQITDIVGVWGLSALVLFISSLSFSIYQVYKEEAAQRGKGSR
                     TFFSPRVFRESIGVFFKPLVVWLCIFFLVLVYGFLVQKNYDDYPQKTIVLIQSNVDPW
                     IGDHETYKHNLHTLIRLTNEALADLEKQGLEADLIVWPETAFIPRIEWHYAKRLYPES
                     YEQVKTLLDFIDSLPAPILLGNDYGVEGYDRLGEFRVLDYNAALLFYPGLNVYPPKPE
                     MYKKNHLVPFTEHFPFEKLFPKLYQALLNGDTHMWELGLEKNILRIDGLGFGTPICFE
                     DTFGYIGRDFVKNGAQAFVNISNDAWSKSLACQYQHLSMAVFRSVENRVPQVRSTASG
                     QTCFIDPNGRIMAMAEPFVETYLIGSIPIIDTVKPSLYNKWGDYMGYIFVILALSLYI
                     INISLGFKKEKE"
     gene            4197..4613
                     /locus_tag="PKW26_00280"
     CDS             4197..4613
                     /locus_tag="PKW26_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOQ92033.1"
                     /translation="MAKQQQNSKALTVLGPETHFSGVLQFKDSLIITGTFEGSIESSG
                     DLEIEKNATCSVDTIYAQSIVVLGQVTGNLYAPEKIEMKQGCKVIGNVNTGKIRIEDK
                     VEFQGRVTMIEKQSDANLFALSPAQYKESLTLIQNT"
     gene            4736..6328
                     /gene="rho"
                     /locus_tag="PKW26_00285"
     CDS             4736..6328
                     /gene="rho"
                     /locus_tag="PKW26_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOQ92034.1"
                     /translation="MAITKGRRFIDTSEEHIESGSDEQIHLDLIDEDKAQEKISDGIT
                     EETKPKATKVKVNSKATKGQAKGAAKEKVVVRPKPKKQNDVEKKQQKEVDLHTEDDLL
                     NRDSKPRLSINDLSRMGMHELRNLAADYGLARDDLAPMKKQELIFVILKAHTEKGGII
                     FSSGALEILPDGYGFLRSPQNSYLPGSDDIYISPSQIRLFNLKTGDTIYGQIRSPKEG
                     ERFFALLRIETVNMNDTRTAQTRIPFENLTPLYPTEKLNLETTTNEVATRILNLFSPI
                     GKGQRALIVAPPKAGKTILMQKIANAITANHPEVYLIVLLIDERPEEVTEMERSIKAE
                     VISSTFDEQATRHVQVAEMVLEKAKRLVENKMDVVIFLDSITRLARAYNQTAPTSGKV
                     LSGGVDSNALHKPKRFFGAARNVEEGGSLTIISTALIETGSRMDEVIFEEFKGTGNNE
                     INLDRKLADRRLFPAFNIKRSGTRKEELLLTEEELQKVWVLRKVISPMDDIEIMELLL
                     DKMKKSKNNEVFLKSMNTSDMG"
     gene            6381..6584
                     /gene="rpmE"
                     /locus_tag="PKW26_00290"
     CDS             6381..6584
                     /gene="rpmE"
                     /locus_tag="PKW26_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOQ92035.1"
                     /translation="MKDGIHPKYEMTKISCVCGNVIETRSTVKDINVEICSACHPFFT
                     GKQKLVDTAGRIERFNKRFNIKK"
     gene            complement(6646..7611)
                     /locus_tag="PKW26_00295"
     CDS             complement(6646..7611)
                     /locus_tag="PKW26_00295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ92036.1"
                     /translation="MRQDNLVFLENRLRKRYKHLKKWARKNKIFAYRLYNKDIPEFPL
                     AVDLYENKEDAKTYVILTFYKQPADKTEDLEAMWIKDRVSICAKILDIPEENIFVKMR
                     ERKKALSQYEKIAEKEKTILVEEHDNLFKINLSNYIDTGLFLDHRNLRLIVKNKAKDK
                     SVLNLFCYTASFSVHAASAGASSVDSVDLSNTYLEWAKENFLLNKLEGENFSFIKSDV
                     IQFLKSCKKKYDLIILDPPTFSNSKSTTTILDINRDYEELIKRTYALLNPDGCLYFSS
                     NSKSLKVELENFSFAKEMQEITKQTISEDFLGKRPHRSWKIVKTP"
     gene            complement(7608..8252)
                     /locus_tag="PKW26_00300"
     CDS             complement(7608..8252)
                     /locus_tag="PKW26_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOQ92037.1"
                     /translation="MIKIPASPSVRRLPSYLHIIRQAHIDGYEYISGTIIAQELQLEP
                     IQVRKDLAITGIVGKPKRGYPVVALISAIEHFLGWDNKNKAIIVGAGNLATALTGYSE
                     FEFHGLEFVCAFDTDPEKIGKKIHGVPIYSMASIKTQVKKLNAAIAIITVPSQFAQET
                     ADMVVETGIKAIWNFTNVKLTLPKGVVSQREDLTSGYAMLSVQMIFDKEKRVLE"
     gene            complement(8254..9330)
                     /locus_tag="PKW26_00305"
     CDS             complement(8254..9330)
                     /locus_tag="PKW26_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92038.1"
                     /translation="MLFKKYVYILNFIFTLTLLCSCMSSPKNQLPLSTEKQTFQTSKD
                     ELKQDTETKEKILTENDKILLESTIEQETKNIENEQLVLQNFIDTLRNLSIKTIQSPT
                     ATIKGKAFNKDFIFQILDNENNPVIDQSITLRYPIAKENDSIFFQETILISDAKGFIS
                     FSPKETDFSCNSFLYLYPVIEGSLQNNAEIEKIIQSEAYKTAFQIKTNLINAGGSLCL
                     ADYDKNNSVVTTNGYSTSALLGSLIRNGFTGVGNLEFYKEIDAGNTEFLLQQVKKLTG
                     AVSTYFIYGTVKYARPPEQVETNKYEVELECEIFCVLLKTNEEIYKTKITVTGFGNTE
                     TAALNDARNNKMNAVLAEKIIYGM"
     gene            9433..10560
                     /gene="alr"
                     /locus_tag="PKW26_00310"
     CDS             9433..10560
                     /gene="alr"
                     /locus_tag="PKW26_00310"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOQ92039.1"
                     /translation="MNNKIATIYLQNFRDNILEIQKQALGKKICLAVKADAYGHGALK
                     IAKEAVKLGLDYLAVSTVEEAVFLRHNDIHIPILVLSIIDESEYKNLILHNLSLVVEN
                     FEQVKALSASANALGKKLGVHLKINTGMGRVGCKPSDVLPLAECIKEMDMLRLEGVCT
                     HLSVSDSLLPEDIAFTKKQIKLFEDTIKTLEQEGIEPGLRHCANSGAIFLHPDAHFDM
                     VRPGISAYGYYASVELKDFCEQKNNCLKPVMELKSKLVKIFPLSKGDSVSYGRTWIAA
                     EDTFIGLVPMGYEDGIRRCLSPGLEVKINERFYPIVGRICMDQLMLDLGKNHSCRAGD
                     EVIFFGPNGGNTADDLAKLCKTISYELLCGIGKRVEKMYLD"
     gene            complement(10856..11419)
                     /locus_tag="PKW26_00315"
     CDS             complement(10856..11419)
                     /locus_tag="PKW26_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92040.1"
                     /translation="MIAVIIAESEDYIKELSQKLDDEGLKIIRYTWLLKALDNLEEIN
                     PQRIYVNALDYPRHWKTLVQYSNGNFLEKKPEIFLYQIDNFGEEEREKAKCLDVKLIA
                     DVKSLRKIEQSLVMINPHTKKLILGTVSLLKNKSIVFNCEQNESVLCLKPSDLIQKAV
                     LKKNKAISEPVLKISSISQKTLHLEML"
     gene            complement(11416..12375)
                     /locus_tag="PKW26_00320"
     CDS             complement(11416..12375)
                     /locus_tag="PKW26_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92041.1"
                     /translation="MKRFLFFSITFLLLFAQSIGANDFSKAEISISFYNKSVYYTGNA
                     VDNPINMYITIENKGPETLRFKLADDRAFSLDFNARTVKNEPLKQTEDIIRKRTTNQT
                     VYFREIAIESGEAYSFVENLKDYVEIESPSVYYIDLVFFPELYKTKTMSLHSNRLSLD
                     VKPLPQASASSSLATAIVSAEVLKRQAISPDKVVEQTIIARQKGLWDQFFLYIDIESF
                     VTKDKQRKVKYTSLSDIERIKMLENYKLDLMQERIDNIIIAIPERFAIEKTMYSQSEG
                     SVSVIQWFNYNTFKEKKRYTYYVRQRDSIWSIYDYTVENLGTE"
     gene            12413..16039
                     /gene="mfd"
                     /locus_tag="PKW26_00325"
     CDS             12413..16039
                     /gene="mfd"
                     /locus_tag="PKW26_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOQ92042.1"
                     /translation="MNTLQTSTINAILSKSKSFQEALSLFSLPLESNAFPCEIEGLQG
                     SLPSFFVSAYAKKNLENLLQSIKYSQLGRYTKADEMLSSGIRDIALIVATENEAQDLY
                     YDIKTIFNDSKKELSPAVYIMPWWGTVPYRPVAPGSIVFGQRIGVLAKIADPSQKNLA
                     DISKIQTRIFIIPQRSLLIPVPPKDSILSLCLDLQLEQKIDPLNIAEHFIRSGYTRVP
                     RVSVKGEIAIRGEVLDIFMPQEDYAHRIVFDFDTITKIKSFDVESQATIADLDALLLY
                     PMKEVLWDEEKIAKLSDYFEKNKSAFVLDVYKEEGEKKLLQKNIAEKLDAFIQALSER
                     GEVEGEEFFYPLVFDEKTSVLDYFSPDATVFYLDYDRMLNALEGIENEFSGLYKRALQ
                     YFNAEGKLPPLPPSVLKLPFEDIFHSFHKKILFRSLQGPSLFEKEKIQIISEPSKSFF
                     GNINYLKESLESLLADDWQVFIFAESENQALRINELLKDFSDELYIIPEPISSGFSMP
                     DIKVLVLKESEIFGRRKQRARSIKHVKSQVIESFVELNPGDYVVHVNYGIGLFEGIER
                     IKSLGNERDYIRLLYAGEEVLFIPIEQVNLVQRYIGNEGNKPNLDTLGSKSWENRKNR
                     VKKSVEDIAEHLIGLYSKRQASRGFPFPKDGEWQLAFEAAFPYVETDDQIKVAKEIKE
                     DMEKATPMDRLVCGDVGYGKTEMAMRASFKAVMGGKQVAFLAPTTILAEQHYETLLER
                     FEKFPVKIAHMSRFVTKAEQKKIIERLALGEIDILIGTHRIIQKDIVFKDLGLMIIDE
                     EQRFGVKDKERLKEMKANIDSLAMTATPIPRTLHMSLLKIRDMSLLETAPQNRQAIET
                     IIDPYSDERVAQAIRQEVERGGQVFYLHNRVESLEEVKYKLEKILPEMMIDYAHGQMS
                     PKELEDIFRRFKMGGFHVLLATTIIENGIDIPNVNTIIIDRADMYGVSQLYQLRGRVG
                     RSDRKAYAYLLYPEGKSLSEIAMKRLQVISDFTELGSGFKIAMKDMEIRGAGNLLGKD
                     QSGDVYSVGFDLYVRLLEEAVNKLMDENYEESGDVLLELDYSGFIPETYIENPVTKME
                     LYKKIASIGSQEEIEGFYMELEDRFGPIPDEVFSLLAIAEIRIICKKLSIASLKERMG
                     VLYIEFAKIAKIDVQKVLYLIQTSSGRIKLDPNKANILQLATGKIGLKEKSAFIREKL
                     ETLL"
     gene            16077..16655
                     /locus_tag="PKW26_00330"
     CDS             16077..16655
                     /locus_tag="PKW26_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOQ92043.1"
                     /translation="MYCIKTGQELRVAISGKSGCGNTTISGLLAKTLHIRLINYTFRS
                     LSEENGLSLNQIIENAKTDDSYDLRVDRRQVELAKKESCVLGSRLAIWMLSSADLKVY
                     LVADEEVRAKRIQEREGGSLQEIIDFTRMRDSEDRERYKRLYSIDNDDFSFADLIIDV
                     SKKSPEEILLEILNELEKRSLIDCTPIKNSRT"
     gene            16659..17483
                     /locus_tag="PKW26_00335"
     CDS             16659..17483
                     /locus_tag="PKW26_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOQ92044.1"
                     /translation="MILSLDEIKAFQTSILEHYYEHNRSMPWRESFDPYEILVSEFML
                     QQTQVSRVLPKYLEWLEKFPRLENLAQAPLAEVLIAWSGLGYNRRAKFLWETARIIHF
                     EKNGIFPHSAAELEKLPGIGHYTARAVLCFSKKSPEVFIETNIRSVYIFYFFKEKSLV
                     KDSELFPLIEKTLYKENPKIWYYALMDYGATLKKKIKNPSRKSAHHTKQAPFKGSFRE
                     VRGIILKELTQEAAESLDLRQLQEKHKIEYQLLYDAAEKLCSEGLVLKEDDVYVLG"
     gene            17521..18075
                     /locus_tag="PKW26_00340"
     CDS             17521..18075
                     /locus_tag="PKW26_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOQ92045.1"
                     /translation="MIYFKKTIKRGSYMKQCGGSTLFLDKTNITTEEIFSIPEGKTEK
                     EVKIFDNLDLTGFESKRDVPGKKVIVSRDNFGIGENQESVAKALKAAGILTIVVSTID
                     GEFKKILKNENIVVVDLDKKTIEDMFKTFSEKDTDCLVLLSEDGTSKIKLISGSLSKS
                     YTFNLEEFYKKLLQEGGLTSCKNV"
     gene            complement(18083..18292)
                     /locus_tag="PKW26_00345"
     CDS             complement(18083..18292)
                     /locus_tag="PKW26_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOQ92046.1"
                     /translation="MEKKIYVAGMTGDECEAKVNTAVSAIAGVQSCVSNAMKAQILVN
                     FDESVAGIEEAITTAIAGCGYDVLN"
     gene            complement(18332..19354)
                     /gene="rlmN"
                     /locus_tag="PKW26_00350"
     CDS             complement(18332..19354)
                     /gene="rlmN"
                     /locus_tag="PKW26_00350"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOQ92047.1"
                     /translation="MTKKAILGLESAEISALIPDEKPFRSRQIYTWLSRGVLDFSQMT
                     NIPIDLRERLETLFFITRSKIQQELKDPDGTIKLQIELFDGHCIETVLLIDADKRKTA
                     CVSCQVGCAMKCAFCKTGTLGFKRNLESYEIIEQFFFLEKICGRLDNIVFMGMGEPMK
                     NLSAIRKTITFLTEPKGRNLSPRRITLSTSGLVDGIYDLADNGPDIRLAISLTTANPK
                     TREAIMPIAKTNKLDALKEATRYFIEKRKKRVSLEVALMKDINTSSEEIALLIDFAKN
                     LDVHINLIPWNPIDELPFSEPSNNEVQRVLKTLQNAGINATIRKKRGRKIGGSCGQLG
                     KLRLDK"
     gene            complement(19369..20493)
                     /locus_tag="PKW26_00355"
     CDS             complement(19369..20493)
                     /locus_tag="PKW26_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ92048.1"
                     /translation="MQSNEDIPSSGNPEVDAKNQEISELSKKGYQYLKDNDIRLAKES
                     FKRILEIDENNNYALVGLGDCERKQNKFKEAVSYYSQCLISHPGNNYALFGLADSYKA
                     LNKYNKAIEIWEQYLLHDNRNITVLTRIADAYRKIRDFKKSKELYLSVLDMEEQNAYA
                     LIGLGHLHYDFKEYRDALYYWTKILEQSEELVDIRILTSIGNCHRKLKTFDKGIDFFK
                     KALEIDQNNFYALFGLADCYRGMNQQHKSIVYWNKILEIDPNNKVILTRAGDAYRNTG
                     DYARATEYYNKALNIEFDVFAVLGLSLISKGEGRYEEAIESLTRLLQADTKNYRLYID
                     LADCYIKLNQKNKAIEILQSFYKTGMRSQAIKEMLDRLQA"
     gene            complement(20516..20857)
                     /locus_tag="PKW26_00360"
     CDS             complement(20516..20857)
                     /locus_tag="PKW26_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92049.1"
                     /translation="MKDLKKTLDHKDSLGGFIKSNEESNKSLSSEEKVLLNRKANILF
                     NEGNYQKAERIFITTGYSDGLSRIAERYKENNQDLQALKYYWLAHNKRGYEPLCKKVA
                     YLITNIIKTED"
     gene            complement(20901..21434)
                     /gene="rsmD"
                     /locus_tag="PKW26_00365"
     CDS             complement(20901..21434)
                     /gene="rsmD"
                     /locus_tag="PKW26_00365"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOQ92050.1"
                     /translation="MRITGGVLRGRKIDCPAGIIRPAMDKMKESVFSILGDIEEYSFL
                     DLFSGSGSIALEASSRGANPVVLVEKDTIKFNTIKKNIQIAPKPIAYKCISAELFLKR
                     NKTQFDIVFCDPPFPYKFHKELIALCSDFSVIHENGLVILHRPSEVFIPDTIQNLKRK
                     DQRTFGRSVVDFFSYKA"
     gene            complement(21431..22717)
                     /locus_tag="PKW26_00370"
     CDS             complement(21431..22717)
                     /locus_tag="PKW26_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ92051.1"
                     /translation="MIQCDCLIIGSGPAGLGAAFSLLEQKPQLKIILLESLPISCGGL
                     RNDCKMNFTFPVGFPLEYWTKQSAKPYLERIENFLNPHILQKDNLEKYTRRSEKIGVQ
                     LLNIRQSHLGTDGGIRLIKELTSKLTKMGVELSFGEALLSANEKAHRAITDKREIQYK
                     HLIVAPGRHGFDFLRKFMEHLDIAYKDNSIDVGIRLETKKEHYPIVEDYYDPKFLFPE
                     RTRTFCTNSGSAHVVQEKYTSSKGETYYSVNGHAFSNAKEPNGLVNFAVLRSVSFTEP
                     IASGQDYAEYLGALAMHTGGGNPIMQRIGDFRMRIRSTRESLNQDLYDFEPTLKSATP
                     GDIALAMPSKFLDSIWRALKKLDTIIPGLLHPSTIMYYPEIKLYANKPVYKNEYFMSA
                     EDIYLIGDGAGTSRGITGAWASGMRAADGILRKIDA"
     gene            22855..23136
                     /gene="rpsF"
                     /locus_tag="PKW26_00375"
     CDS             22855..23136
                     /gene="rpsF"
                     /locus_tag="PKW26_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOQ92052.1"
                     /translation="MRKYELTTIFPVDEELYKTALDSVKQVLSEFGAEVESEESLGDR
                     NLYYMIKKRNKGRFLLLNIKVNPAKVVEIEKKFKLIDSLLKYMFVRVDE"
     gene            23151..23633
                     /gene="ssb"
                     /locus_tag="PKW26_00380"
     CDS             23151..23633
                     /gene="ssb"
                     /locus_tag="PKW26_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOQ92053.1"
                     /translation="MADVNQVTLIGRLTRDAELKYTSNGFAIASFAIAVNRRRKNGDQ
                     WVEEASFFDINLFGRSAEGLKQYLVKGKQIAIEGELRQDRWEQDGQPRSKVVVIANTV
                     QLLGGNPGSSGSQQYGGAEGYRPEYQQKTASYQAKKEVQQNSYEPSYDDSDFPEDIPF
                     "
     gene            23740..24003
                     /gene="rpsR"
                     /locus_tag="PKW26_00385"
     CDS             23740..24003
                     /gene="rpsR"
                     /locus_tag="PKW26_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOQ92054.1"
                     /translation="MQDVMKDVDDKSMRSRPKTFFRKKVCRFCAQKAKIDYKDPDSLK
                     RFITERGKILPRRITGTCAKHQRRLSLEIKRARAICLLPFVTE"
     gene            24078..24656
                     /gene="rplI"
                     /locus_tag="PKW26_00390"
     CDS             24078..24656
                     /gene="rplI"
                     /locus_tag="PKW26_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002678440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOQ92055.1"
                     /translation="MKVILNTDVKHLGEEGDVKEVANGYARNFLFPRNLALPYNDVTH
                     EYFESRRAEIEAKKEVKRNDSRSLRDRLVNHLVEIPMPAGANGKLYGAVTSQTISDAL
                     KKAGFEIERKRIEVPGLTIKNLGKTTVIVKLYEALTAEVEVLVVNQDDVKDAKEQKKT
                     EETAEVKAEEKSQAVEETPAEVEEQNTENTEA"
     gene            24672..26006
                     /gene="dnaB"
                     /locus_tag="PKW26_00395"
     CDS             24672..26006
                     /gene="dnaB"
                     /locus_tag="PKW26_00395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOQ92056.1"
                     /translation="MSQSIKYKVPPHNLEAESATLGALLLDWEAVGEVIKYLRPDRFY
                     SLQNQKIFSGIIELYNKSQRADLITLIEQLKSTGSLEEAGGLSYISSLTDTVPTSANV
                     EHYAKIVLDQAVRRDLLKLSASLAEFSHDETQSSRYSLEEAQKEIFNLTDLNATHTIK
                     SSGEIVNHAIDMITKLYENKNDITGVPSGFYDLDKMTSGFQNSELIIIGARPSIGKTA
                     IALSMAQNIAIERKIPAAFFSLEMSDTQIVFRLLTQESRVRSEFLRTGKLRQEDLQKL
                     FDAAGKIYEAPLYIVDSPNMKLLDLRALARRLKAQHDVKIIFIDYITLITSENTSLPR
                     HEQIAEISRSLKSLARELEIPIVVLSQVKRDSEGKQPSLADLRESGSIEQDADVVMFL
                     HRERTKAGLDEQKNLSIETQLILAKQRNGPTGIVDILFFPNYTKFDNLTRHA"
     gene            complement(26014..27930)
                     /locus_tag="PKW26_00400"
     CDS             complement(26014..27930)
                     /locus_tag="PKW26_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOQ92057.1"
                     /translation="MNKQKKTILFLSALFLLLASIPLFSQSELALSHKGLITSIVESN
                     GFFYTGAVDGFVIRWDANLNQERLQVSDVEIQKVAVHPDGNIIAIYETDGFSVYRLSV
                     WDWQKKERIYAKRLQEKITSLSFSQKGSYILIGNGNFDGLIILDAVYGIQMQMLNTSI
                     AQVSLAETSATEATLVSYSSNGQFTYASLDDGSIKSTIKTENNIKSIIKTESNLEKGQ
                     FLDLNKRFFAGFKNKKFFIIDATNGKTQKTISVEKPVLIPGYSNQEFYLIDEVSAENY
                     SLKKISLEKGDTQFEINTESHFDLAVPPSNAVWLAGTIFIGTGDGSLYSLNTSAKFLF
                     AKKETQSSQVQFLDITKDSNSFYILTNEALYRIGENEKELEKIVDNQNWKNILVHNDS
                     VYFWSKGQRDAVVSWNVLENIFQELFIPSQSLENLKVYDSELIALLSSTTVLSYNFQT
                     KKTETLYTGTGINDILLKDESLFISKSAATNPRTPLIEINTKTKETVLLPLTNDFIYA
                     LDQDTDNKSPYLYGLSINSNNDSFTTELFYYHKDRKTYASILRLQDENTNAFLTIQES
                     ILYTNLGKSDVRVVNLKTNRTQILPKTKALPQKILAIGQKLLVLNQDGTITWYQSNKA
                     IKEWNANISESLRL"
     gene            complement(27947..29308)
                     /gene="rseP"
                     /locus_tag="PKW26_00405"
     CDS             complement(27947..29308)
                     /gene="rseP"
                     /locus_tag="PKW26_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOQ92058.1"
                     /translation="MSIIYGLLGLSLLVFIHELGHFIAARALGVEVETFSIGMGPVLL
                     RKKIKTTEYRLSLIPIGGYCGMKGQKDFQFALDNKLDEIRGEKGSFYASNPLRRIIIA
                     LSGPLANFVFAIFAFALISLIGYSFYTSSNKIIIANEVYPELHSTAAEAGLKTGDRIL
                     AINNKNIHYFSDISDIIAISANKVLDITVDREGELFHFQVLAELDTSSGAGKIGIVNW
                     VNLLITSIQSGSHAESIGFKIDDIITAVNGIETKNTSDLEKVLNSLKVAELDEPIQFT
                     VMRENKNIVLETDSSLLNDIQNLGLQFEFFKVHTPKKNIFTAFSSGVKETFKMIALTF
                     KGIGLLFKGLDPKEAVSGPLRITLMFGDTVKDGFAASFSIGLISALSFLALISISLCI
                     MNLLPIPILDGGLILFATIELISSKQVSPKILYYVQFVGLFLILILFAFALFGDINFI
                     FKN"
     gene            complement(29305..30408)
                     /gene="dxr"
                     /locus_tag="PKW26_00410"
     CDS             complement(29305..30408)
                     /gene="dxr"
                     /locus_tag="PKW26_00410"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOQ92059.1"
                     /translation="MKAITKKKVLILGCTGSIGQNSIALMKEYPNDFEVVGLSAHRNE
                     AKLKTFGKECNCTNLFLSENNEEGFAEFISQTKADIAINGISGFAGLLPSVHCLEAKM
                     DLALANKESIVSAGDLLQSIARKNNKKIIPVDSEHSAVFSLIQGLGKNSVKKILLTAS
                     GGPFRLWEKERFPSIRVQDALAHPTWSMGKKISIDSATLANKGLEVIEASRLFSLDAK
                     EIQVLIHPQSIVHSMVQSKDGAYYAQLSPPSMKQAILYALNYPEIKENNLPSLDFSQD
                     LALNFSPPRMNDFPMLALAYEVLEKDGALPIVYNAANEIFVHAFLEEKIRFIDIPVLT
                     EKILNGNWSMKPNHLEDVIQIDRIAREKAGALI"
     gene            complement(30410..31255)
                     /locus_tag="PKW26_00415"
     CDS             complement(30410..31255)
                     /locus_tag="PKW26_00415"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOQ92060.1"
                     /translation="MLQRILLFFIGVPVLLLLVYIPFKIHLFLHIALCIVTILASLEL
                     YDIFSKKIALQAKAFLIFLTSLFPIISYSAAIFSLPDFTINIVLIITTITLVIFEIFY
                     TNKKEDSVFVLSIERISASFFIIFYCGYLFSYISKIALLPYSQFYLVAFLAFIFGCDS
                     FAWLFGMLFGKGNRGLFTVSKKKSIAGFVGGILGTILLGFIAKLLFPDAFPGSIYKML
                     ILSSSMAFMAIMGDLFESILKRSAELKDSGFIVPGRGGLLDSIDSMIFTVPLYYYFIL
                     LFYGA"
     gene            complement(31275..31973)
                     /gene="uppS"
                     /locus_tag="PKW26_00420"
     CDS             complement(31275..31973)
                     /gene="uppS"
                     /locus_tag="PKW26_00420"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOQ92061.1"
                     /translation="MAETSESSSSVPKHIAIIMDGNGRWAQKQNLPRTTGHKKGLDVA
                     KQIVKTARDAGVDYLSLYAFSTENWKRPKEEVSYLMNLITTHLKEEFNFYKENQIRVQ
                     HIGRQDGLPKKVQKELQETIDFCKNFQGLTVVLAINYGSRDEIIRAIKKIENIQSLNE
                     ENFHNYLDYPELPDVDLLIRTGGEYRISNMLLWQSAYAEFIFTKTLWPDYTNECFLND
                     LETFKTRQRRYGAL"
     gene            complement(31973..32524)
                     /gene="frr"
                     /locus_tag="PKW26_00425"
     CDS             complement(31973..32524)
                     /gene="frr"
                     /locus_tag="PKW26_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOQ92062.1"
                     /translation="MEEIKNATEKMQKTIAALKDEYNTIRTGRASAALFDKVRVEYYG
                     DKTPLNQVATISIPEARMVVIQPFDKTLMGEIEKAIQKADLGFNPSNDGKLIRIAIPP
                     LTAERRKELVKQAKNTAENARVSIRNTRRDGNDDFKKQQKEGLISEDALKTLENALQK
                     ETDEFILEINKILEEKEKEILEG"
     gene            complement(32589..33425)
                     /gene="tsf"
                     /locus_tag="PKW26_00430"
     CDS             complement(32589..33425)
                     /gene="tsf"
                     /locus_tag="PKW26_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOQ92063.1"
                     /translation="MEIKAQDVKALRERTGAGMMECKNALVQTKGDAKEAEKLLKEKG
                     LAAVEKRAGRATQEGKIFIQNNETNVVICEVTCETDFVSKNAEFIEIGNNIAKKALEN
                     KMTEITDDLSNMLLGLATKIRENMKITRLALIDIPANAIFSSYIHSDGKTGVVAIVSA
                     DPASAKDSETVKEFAYDCCLHVAAFNPPYIRREDVPASEIDELKEIFTKQTAELDKPE
                     KVKEGIINGKINKHYAEVCFLEQAFVKDDKMTVARKIEAVGKEVDAKLNIEKVVSYVL
                     GA"
     gene            complement(33442..34320)
                     /gene="rpsB"
                     /locus_tag="PKW26_00435"
     CDS             complement(33442..34320)
                     /gene="rpsB"
                     /locus_tag="PKW26_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOQ92064.1"
                     /translation="MAVVTMKNLLESGVHFGHQVKRWDPRMKKYIFAERNGIHIIDLQ
                     KTIVEIKAAYEAVRKVTSAGKPVLFIGTKKQAQQAIAKEAERCGMFYVNNRWLGGMLT
                     NFSTIKKSILRLKKLEKMEVDGTFANLTKKEISGILKEKEKLEKNLGGIKDMKELPGI
                     IFIIDTHKEAIAVAEAKKMGIPIVAVVDTNCNPEGIDYPIPGNDDAIRAITLFISIIA
                     NAVIEADNETGLRIIEDLNDDEEIETDVAKEPEEEEIVDFKNYSPSEEDVKEEKKESE
                     TESKEESLVDEEDLYK"
     gene            complement(34498..35091)
                     /locus_tag="PKW26_00440"
     CDS             complement(34498..35091)
                     /locus_tag="PKW26_00440"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOQ92065.1"
                     /translation="MEPIILASSSPRRQELLNMLGIPYQVVMPDIDETIPEGMNAEEA
                     AEHFATKKIHAALKLMPQGQTIPWILSADTLITLDGKIYGKPNNQEEARSFLKYFSGK
                     EQEIISAIAFFNGKLNYLSTRINKTKLLFSKLSNKEIDWYINTGEWHGVAGGYRIQGL
                     ASCFIEKIKGSFTSVVGLPLFEFYDILKEQGYPYIKD"
     gene            complement(35123..35803)
                     /locus_tag="PKW26_00445"
     CDS             complement(35123..35803)
                     /locus_tag="PKW26_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOQ92066.1"
                     /translation="MKLQKIAACTLIVFLALGSVIAQRSETTVEQEYLNSVEDVIVKE
                     LAAADDRDSKLVSLQYLELALDSGRTSPDMIYALEMLAGEGSFTQVRTNGRLANNFPD
                     IRAKACDLLAKAKTEESKHILLKVILADNEPMVTTAAIRSLGEIGLNDKNDVLEAISW
                     TEKKYAILNPTSSLALEILNTYEKLAPTATDNTSMIQSISAIATNYNYVTPVRTKALN
                     LLKRLTGR"
     gene            complement(35886..36518)
                     /locus_tag="PKW26_00450"
     CDS             complement(35886..36518)
                     /locus_tag="PKW26_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92067.1"
                     /translation="MKDCTKKVILYLTFSLIFVILACKTADTDQSFHDSNIAEKEEEI
                     ILELPPLLEEIIVEEEIKPIEKTAEIDEEYVRSTSGLKDKSITQDTFLADKKEILEKI
                     DALAQIMHAQDYGKWLYYLSPSSKAYWQNKKNLTDVSSRLPVKGYTLKNLEDYFRMIF
                     IPSRQNSKVDEIRYISSTLVKAVQVRGSQDVVYYVFIKINNTWLLELDTL"
     gene            36601..37080
                     /gene="coaD"
                     /locus_tag="PKW26_00455"
     CDS             36601..37080
                     /gene="coaD"
                     /locus_tag="PKW26_00455"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOQ92068.1"
                     /translation="MVSALFPGSFDPPTFGHMNIIERARKIFNEIHVVIAVNSQKKYL
                     FSEEERFLMMKDLTKQWDNVHVATSKTLIVDYAKKVGATVLLRGVRNISDFSYEFDLS
                     MLNKGLDATIETIFLPTDPEYFVLKSSAIKELAFYDGDVSTMVPELVAKALKEKYKL"
     gene            37250..37432
                     /gene="rpmF"
                     /locus_tag="PKW26_00460"
     CDS             37250..37432
                     /gene="rpmF"
                     /locus_tag="PKW26_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOQ92069.1"
                     /translation="MAVPRAKTSKARTKRRQHINMKLQAPNLVECGNCGNLTMQHRVC
                     PKCGFYRGKQVITPEA"
     gene            37452..37685
                     /gene="acpP"
                     /locus_tag="PKW26_00465"
     CDS             37452..37685
                     /gene="acpP"
                     /locus_tag="PKW26_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOQ92070.1"
                     /translation="MDELFTQMQKLIANKLEIEESKITLDSSFRQDLGADSLDTYELV
                     YAIEEELGVSVPDEKANEFETVRDAYDFIKSQK"
     gene            37746..38483
                     /gene="rnc"
                     /locus_tag="PKW26_00470"
     CDS             37746..38483
                     /gene="rnc"
                     /locus_tag="PKW26_00470"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOQ92071.1"
                     /translation="MPYTLQNFQYSRKTRLERFQEEVGLSFQSLDLLNLAFIHKSYAN
                     EKPSFAKQKHVYNERLEFLGDAVLDLIIAEHLYTIMLLSPEGDLARARSAIVCEDSLS
                     EVARKMKLGSCLLLGKGEEAGGGREKNALLADTFEALIGALYLDSGLEKTRLFILSSM
                     QDRIRNVLTQKVGKDYKSTLQEYVQKEYKKTPEYKLHSESGPDHDKSFFIEVLVNDQS
                     FGPIKGKNKKEAEQGVAKLACDFFKLL"
     gene            38553..40349
                     /locus_tag="PKW26_00475"
     CDS             38553..40349
                     /locus_tag="PKW26_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOQ92072.1"
                     /translation="MVEQKSKRKKRIFPHLKNSSFFLHIFYSFIVGGLIPAIFLSILL
                     GLLSVQILSTAFKNQTDEAISKASLLTEELFKSIEATALFIATHKDVLTWAESEKNDP
                     NKILAVNKLISEVIDSPYIQAYLLPLDGSDTISRRQIPEEYKQGLYANWGLLGALSKI
                     KTEGTKPVFYSLPHYNMQTPVSFAVGYPIFSKSLPIAYLILDVERDELSQRVGLAAFS
                     SGFISDLFLVDKTNTILYSVNDAIKEGSFYEPKEDGSFFSLDKHLSHDLRLFAYYPLY
                     TVHYFTQKMKNASFFVVFVSFFLFLMMAILLSRSIAYPIQMLTKTMKHVSYGNLDIRC
                     DVKFTKNAEMSFLIESYNYSLDQIRELYHNRIAQERSLRHAQVQALQAQINPHFLNNT
                     LNSIKSMALLEGNKDVADMITSLARIFRKSISISSEFTNLADSIAIARDYFTIEAKRW
                     PGRFTLTENIEGELYDAVLPHLVLQPIFENALMHGLEKKEGKGTIWLRIWLCGGDLFI
                     EVKDDGIGIKKENLKKIQKKLDEARLHPELISDLQTKTNMSGSSGIALINTHRRLSLI
                     FGKTYGLQIESQENFGTKVLICLPYKKEGSLV"
     gene            40342..41157
                     /locus_tag="PKW26_00480"
     CDS             40342..41157
                     /locus_tag="PKW26_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ92073.1"
                     /translation="MFKVLVVEDESLVRRALVSLTPWQDLGFEFCAEAENGSAALSLF
                     MQHQPQVIITDIRMPVMDGLDFIQKVFDYVQKEACPEPEVIILTGYSEFEYSRKALML
                     GVHEYVLKPVEEEILYNSLERAKQRLLDRQKEKERVQISLFEKIQSADNDDTGIAFNY
                     VKESIALIENHYVLGITIEEAAQKLGISSGHLSRLFKQSTKLTFNEYVRNVRIEKAIA
                     LLKDQSIKIYEIADLVGYLDVKYFTQTFKKITGLTPSEYRYRFFQANQAIDTE"
     gene            complement(41087..42106)
                     /locus_tag="PKW26_00485"
     CDS             complement(41087..42106)
                     /locus_tag="PKW26_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ92074.1"
                     /translation="MFLFILLLSCAKQDSNKPLKKTNELIILSPHPEDMIDFITKEFH
                     QRTGIKITVIREGTGELMQKVANLPYLKADVFWGGGIDSLETIKDHFLFYESEVTNAI
                     YPTYKSPHSLWHPYSVLPMVIIYNKSLIPPDLWPKSWDDLLNPFFKNRIIMADPQKSG
                     SAFTILTTLLYVKNTNKNVLFGGRDFLNALIGQLGEEGITQNSNTILKAVSTGDCFAG
                     ISFEDYAITLEKLSSNVAYIYPSEGTSLLPDGVALLKNAENLIEAKAFIDFVLDEDVQ
                     KLLSPRWQRRSVRTELEKSNKKIMSPLIQYPTTDAANSRELILYQWLDLLEKNGIDTH
                     SELNP"
     gene            complement(42132..42974)
                     /locus_tag="PKW26_00490"
     CDS             complement(42132..42974)
                     /locus_tag="PKW26_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ92075.1"
                     /translation="MKTLATTFNTLIWLFLLTFAILILLPLVFMFTASIMDAKEIITM
                     PYRWIPSEIKWANYARAIAGNDGSYQFVRNIANSLIVSISIMVSTIFLSSMVGYGLAK
                     FNFKGRNIVFLAIMATMMIPFETIMIPLFMVALKLGIQDSYRGLIIPFMLNAFAVFQM
                     RQYLLTFPEDILDAARIDGMNEPSIYAKIILPNATPVIATVAVLTFRQQWDTLLWPLL
                     VTQREEMKTIPAYIVKFAAEKYADEGAMMAVAVIASIPVIALFLSLSKYFVGGSSTYA
                     AGKE"
     gene            complement(43034..43942)
                     /locus_tag="PKW26_00495"
     CDS             complement(43034..43942)
                     /locus_tag="PKW26_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ92076.1"
                     /translation="MKLKAKPSYEQKKARWGWIFVSPGIVFFSIFSFFPILNAFWTSL
                     LNKKLVSLKKANFVGLTNYIKILTNYDFWNSMKATLVFTVGCFIPLVIIGLVLAIFIH
                     SRKRFNRSLQLVYYSPAVLSSVVAALIWFLMLQPTGLLNQTLNTLFSTSGVDYKWLAS
                     GPMVRLSTIIVYFWKYIGYFAILYITGIGKIPTAIYEAATIDGANGWNQFKLITIPLL
                     KPTTLMVSIMAMIQCLKTFSTQYLFTQSGAPSAPINVITLNIYNTAMRDNNLSRASVM
                     SVILFLIMLLLTIVQFKTSKSDDVSF"
     gene            complement(44005..45300)
                     /locus_tag="PKW26_00500"
     CDS             complement(44005..45300)
                     /locus_tag="PKW26_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ92077.1"
                     /translation="MKKMMFVLMLCIIASPLVLFAAGTKDAKATGPIELTIWTHEDVN
                     RKAIEEKYIKEFEAQNPNIKVKYVVYPSTEIHKVIQAGFAAKNGPDIFNLEIQKAYPF
                     LAEGFVAPVDLASAGYKSHNEILNSYMAGMLDPVTVDGKVYGLPLELTNWAIYLNKKI
                     FRDAGLDANKDYPKTWEEVMTVSEKIVKRDGSIITRRGFDFRYGDYLISWLPMVEQLG
                     GKLISDDGKTAIVNKDAWVKVLQYMADFGPNGKNLGSPTYTAARKVFDNDNNEIAMHL
                     SGLYQEQRMASANPDFFNSDDWMVIPYPTFAGGKQVPNTYYGHYYMVNSQVSKERQQA
                     AWKLVSFMLSHGEEYLEKVALVQPTNTLFNSATFKNMPFSKVFKDELEIAPVVYYGEA
                     SQQINDLIKEAIESVMLNKVAPARAVDTLQTKVQEVLDN"
     gene            complement(45531..46025)
                     /locus_tag="PKW26_00505"
     CDS             complement(45531..46025)
                     /locus_tag="PKW26_00505"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ92078.1"
                     /translation="MSKLILCCGKIASGKTHFCSQVKNKKNYFVFNTDEWMIHFYGES
                     PDCSVFHERLEKCTDLMYKIASDLLHKDINVVLDFGFWTAKERKKCLDYFEKMNPNSK
                     RIILYFPIDDIKQRSHLDKRQKKMPEASFYFSDQKLLFFNEKFETPTEKELILIDDFA
                     KILV"
     gene            46159..47517
                     /locus_tag="PKW26_00510"
     CDS             46159..47517
                     /locus_tag="PKW26_00510"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HOQ92079.1"
                     /translation="MAFPKNFIWGAATSSYQIEGAYKEDGRGLSIWDAFSKSPGRVFN
                     GDTGDIACDHYNRYKEDVALMKKIGLEAYRFSIAWSRIFPEGSGRVNEKGLDFYSRLV
                     DELLEAGISPWATLYHWDMPLQTYYKGGWLNRETTDDFAKYTEVIVKKLGDRIKNWMT
                     FNEPQCIIGLGYETGEHAPGLKSSDYEIATALHNHLIAHGKAVDVLRSYGGDAFSIGY
                     VPTCQTTIPKDSSPEAIDIAREALFSLQDFRSPIWTISTTCDPVYKGEYPQDVLKRIE
                     DKLPKGWEKDLENVGGKTDFLGVNLYFGYVVDKVEGKIQIVKPKPGMAQTALRWGVEE
                     ETLHWAPRFLYERYKKPVVITENGLSNTDWKQSDGRVNDPARIDFVKRYLVGLHKAIE
                     NGADIAGYFHWSLMDNFEWAQGYRDRFGMIYVDFETQERIIKESGHWYRKVIESNGEL
                     IL"
     gene            complement(47588..49699)
                     /locus_tag="PKW26_00515"
     CDS             complement(47588..49699)
                     /locus_tag="PKW26_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92080.1"
                     /translation="MKKKTKKMHPLVQGILGILLFSLAAASLFFFFLQIDFRKIPFLG
                     KTIERFSKTSTRIIDEKESLFLQSASFNIDFLLKLETKDGVYLAIYPYEVLMGIDGNK
                     ILFEKNQDTQDCYLPVPEILQSELADTDSILEIVNTIHSSDFSYNTIISPIKKAFEQK
                     AIDEALENNYFTDSFISRTKKNIEDLYPEKIFFVNEKEIREALENTMQRIDAHFFPAE
                     FKVQKDFFDHFDSNLLQENNQKNKIENEIENEKYFTRDDSQIDALRFGLVKNFAKLPS
                     LSYATFKNKNLLKQDKAKLIASWLNPLDDNTSKTFLFAGEDGYEKAITLFQDGSISYI
                     EPKGMNIDERNAFYSFPLAYLAMSIEYKSFGQDEKKTLNEYVQFSSLYDQALEYLEEE
                     KYTRLDLQLRQLKEWKHKEEKAIQLLQALFDAAVFYQYNPVPMEAYENFNALLFLQAH
                     LHQLKHFNSPENRSKALKEMETISRNNKDTLTIQSLKALFLKNKTLLKLSPSEIQEYE
                     NDIIESGIIISKELFSSLDENGRKSYILNTFKDLTKNNPQRIEIEEVNHTSFLFLDEA
                     YDELYTKKSKKIFDIKKRIESKLENKTSLVILFPQKKSFTTQFHCLIFHSDSLSFIEN
                     ITSPFFFQEAKVFPYEKISLSSKAFLLGSKAFENDIVLAVLETIHKAYYFDQIYHIEL
                     DKNIRAQLLKEVRDKMDRPQY"
     gene            complement(49683..50660)
                     /locus_tag="PKW26_00520"
     CDS             complement(49683..50660)
                     /locus_tag="PKW26_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92081.1"
                     /translation="MKTKIAFFVFALIIISPLLFYLAYKKQTKDASFEAFNQYREQIP
                     LHNESPVLVYKSVDFYKYIVYGTKTDNVHSDKLIVYLVKGTASFGFDLSKIKIDTERS
                     NPKNGKLYADFNSESLMPVFVSISIPEAGIQEVESIEPKPYTEDEAKQIAKTLAIVGS
                     GLGGFAGGIVGSTSAGIAASLLDGGLLPKPITKLTGASIGAGLAALATGITSYLCTKN
                     FLLDYEATGYGFTEVQHILQASKGLIALELLEGANLIHTKEDLFHWENTKRAEQEKAI
                     LRASERLFQRFGWKEIQLSFHYPENKESTTSSIQTQENDAGDFDNEKEN"
     gene            complement(50724..51518)
                     /locus_tag="PKW26_00525"
     CDS             complement(50724..51518)
                     /locus_tag="PKW26_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ92082.1"
                     /translation="MIALDTVHKTFNEGTINERKALIDINLKLEEGDFVSVIGGNGAG
                     KSTLLNLIAGVYEVDSGHIYFDGIDVTEEKEHKRARFLGRVFQDPMLGTAANMQIDEN
                     LALAYRRGKRRSLAWGIRPAEKERYKKELAMLDLGLESRMTTKVGLLSGGQRQALTLL
                     MATIQKPRLLLLDEHTAALDPKTAQKVLELTDEIVKREGLTTFMVTHNMKDAIRHGNR
                     LIMMSAGKIIYDVRGEEKRKLEVADLMQKFQSSAQDAIINDRMLLS"
     gene            complement(51515..52456)
                     /locus_tag="PKW26_00530"
     CDS             complement(51515..52456)
                     /locus_tag="PKW26_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ92083.1"
                     /translation="MGIFLAIQGAASQGVLWGLMTLGLFITYKILNFADLTVDGSFAL
                     GGSVSAILITSGFSPYLSLVIAFFAGSLAGLVTGLLHTKLKIPGILAGILTMIALYSV
                     NIRVMGQANIPLLGQETMLTLTSSFGLSKTLGSLLIGSIFSAIVIAILYWFFGTELGS
                     AIRATGSNEKMVQALGINTESMKNIGLMLSNALVAFSGALVAQSQGYADVGMGTGTIV
                     IGLASIIIGEVIFGKRFSFWYRLMSVVFGSILYRIIIAIVLQLGMKSTDLKLLTAVIV
                     ALALALPVINEKMTRHQKRKINRLQKKTEANKKASIQ"
     gene            complement(52467..53444)
                     /locus_tag="PKW26_00535"
     CDS             complement(52467..53444)
                     /locus_tag="PKW26_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ92084.1"
                     /translation="MKRNVFRILFLSFIVVSMSGFFLSCANKKDDKIKIGALQLVEHV
                     ALDAAYAGFVDALAEEGYIDGESIKIDFQNAQGEQANCQTIAQKFINDKSDLILAIAT
                     PAAQAVANLTETIPILITAVTDPQSAMLVKSNEMPGTNVTGTSDLTPVAAQIALIKKL
                     VPEVKTIGFLYNSSEQNSKFQIDLAKKEADALGISYLDATVSSSNEIQQVVQSLVGKV
                     DAVYSPTDNMIAAGIPTVAGVLNPAKIPFIVGEEGMVQAGGLATYGINYYALGKQTGK
                     MAAEILRDGKKPATMPIQYLQGGDFALNEASAKEIGIIIPEELLLELGK"
     gene            complement(53565..>53851)
                     /locus_tag="PKW26_00540"
     CDS             complement(53565..>53851)
                     /locus_tag="PKW26_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92085.1"
                     /translation="EGKEILSAHIRESYLSLSLLETKIEYYQLKQAVNNRELLDKKTQ
                     MEMGIISEADFIETMILSLGDLAEAYALLAEHQSKVLTVEMLLGKEAWNP"
BASE COUNT        16496 a         9233 c        10229 g        17893 t
ORIGIN      
        1 gcgtatacga gtcttatctt tgacgaagca catggcattg aagattctgc aacgagcttt
       61 ttttcttcga gtttaaatcg cttcactgtg ttgaaacagc tttctcttct tgcacgaagc
      121 aggaaaaatg cccatacggg tcttttgttt tcattagatg tgttatcaaa tagccctgaa
      181 tatcttcaag aatgtttagt taagatgcaa gatataaaaa ctgcactgac gcattttgaa
      241 actgaagcgc tagagctcat ggaaaaagac tacgcctttc gctttacggc aggaacagca
      301 tttcggctaa ggtctttttt agaagctgtt gcagatttgc atcgagcctt tcattctttt
      361 tttacctcat ttcgagcttt aatccaaggt attgatgaag aggataagag tttgccagcg
      421 gtttgggaag gaaagcaaat ttacaaacgg cttgagtaca tcgctgaggt ttgtgaatct
      481 tttttggatt gggatcaaca agaagactat gtgttttgga tggaaaaaag acgcctcagt
      541 tttgcgcaaa ataagaatga attgtttagc aaggaagatg gccaaagcta tccggttata
      601 cacaaaacac ccttgtctat agccaaaacg atgaacacag gtgtcttttc acaattcgaa
      661 acggtcattt gcacttccgc taccttacaa acaagctcag gtttttcttt ttggcttcag
      721 agaaatggtg gacaatatct ttcaaaagag cgacttattt gtgccgcctt tccgtcaaat
      781 tttaattatc gagaaaatgc ggtcttggct atacctaccg atatgccgct tcctgattca
      841 cctctttttc aagcaagtgt ggaaaaaaac gtgctaaagc tattgcaagc atctgaggga
      901 cgcgcgctag ttctctttac gtcttttgag tctttgcgaa gcacagcgag ctatttgcgt
      961 gataatagtc atctattgat ctacccactt ttagtgcagg gtgaagcaga tagatttaat
     1021 ttactaaagc aatttaagga aaaaaaagat tcgattttgc ttgcaacctc ttctttttgg
     1081 gaaggagttg atgtgccagg cgattcactt tctcatgtta taatcgtaaa gttgcctttt
     1141 gctgtaccaa atgaacctgt ctttgctgca cgcagtgaag ctatcgaaaa gcaggggaga
     1201 agttccttcg ccgagttgag tcttcctgga gcgattatag cctttcggca gggttttggg
     1261 cgtcttatga ggcgaaatac agatcggggg ataataactg ttctcgatcg ccgtcttttg
     1321 gctaaatcct acggtagatt atttttagct agtttgccag agacaagaca attgttttca
     1381 ccgatggaag atcttatttt tgaaattaaa agctttttaa aataaaaaaa accaagattt
     1441 cagatgactc ctacaatttc ttggtttttt tgataataaa aaagtatctt cggtagctca
     1501 aaagaatttt tgagacaacc tttaattaaa gtcctttggg cggcgttcaa cgcgtttgca
     1561 tttttggcat tcggcaatgt ttttaatctt gccaacatca aataatgttt tcattgaaac
     1621 ttcaccaccg caagagcaca taaatttttg tgtagcaaca ctttttcttg agtttttact
     1681 ggcaccagcc attttacgcc tccctgaatg gtttgtaaca atactatact actatgcaat
     1741 ttttatgtca ataagctttt ttacgctact tgactaatac gggtggcttt ggtaaaatat
     1801 cgaatagttt ttaaatgggg gttctccttt ggcggttaaa aatgcatctg caaaaaaaag
     1861 acataagcaa agtgaagatc gaagattacg aaataaggca gtaaaaagtg ccacaagaac
     1921 aagcgcaaga aaatttgttg aagctgttca tgtaaaagat caagctcttg cttcagaaaa
     1981 acttggtata cttttaagcg agctcgataa ggctggcaaa aaaggtgtta taaaaccaaa
     2041 tactgtttct cgcaaaaaat caagaatgat gaagctgtat aatgcttctt ttgagactgc
     2101 aagcgcgaaa taatgtaaat tataaagggc tttgcccttt ataattttct cgtatcgatc
     2161 ctattctgag gtgataatgc aatccaaaaa agttaccaaa tatgatctag tagaagcaat
     2221 ctatgaagat acaaactacg aaaaaaaaat aatacaagat attgttgagt cattcttaga
     2281 gcaagtaaaa gaatcattgg ttcaatctaa gactatcgaa cttcgtgggt ttggaacatt
     2341 tgaattgcga cttcggaaag gaaaagcagc ggctagaaat cctaaaacag gggagcaagt
     2401 gagtgtagca cctcattatg ttgccgcttt tcgctcagga cgagacttaa aaaattcatt
     2461 atgggcaata ccaacaccaa aaaattaatg caaaataaaa caaagaccat gttgggttta
     2521 cagcgatttt ttatacactt tgcattattg ctttcttcag cacttctttt ttcactttct
     2581 catccaaata gtatagggca aaaaggcttt ttctttttag cctattttat gtatctgcca
     2641 ctgcttattt tgatttctcg cctacgttta aaagaaagtt ttttctatgg atttttgtat
     2701 ggcattattg cctattgtat ttttacctat tggctggcaa catttcaccc catgggcatt
     2761 ttggttattt cttttttata cggttttcaa tacgccttat tgtttccggt tcttgtaagc
     2821 gctcgaatta ttctagaaag gcaaaaactg acgagtcttg tctttgctaa aaaactactt
     2881 tatatgcaat ggcttatctt atctgcctat gagtttataa aaacacttgg atttgctggt
     2941 tttcattatg gccttgcggc atatactcaa tggagtcttc cccccattat acaaataaca
     3001 gatattgtgg gcgtttgggg actctctgcc ttagttttgt ttatttcatc tctgtctttt
     3061 agtatatatc aagtatacaa agaggaggca gcgcaaaggg gtaaaggcag tcgaactttt
     3121 ttttcgccga gagtttttcg ggaaagtata ggcgtatttt tcaaaccttt agtagtatgg
     3181 ctttgtatct ttttcctcgt tcttgtatac ggttttcttg tacaaaaaaa ttatgatgac
     3241 tatcctcaaa aaacgattgt attgatacaa tcaaatgtcg atccatggat aggggaccac
     3301 gaaacatata agcataattt gcatacattg attcgattga caaatgaagc cttagcagac
     3361 cttgaaaaac aaggtttaga ggcagattta attgtttggc cggaaactgc ttttattccg
     3421 cgcattgaat ggcattatgc aaaacgctta tatcctgaaa gttatgagca agttaaaacg
     3481 cttttggatt ttatagatag tcttcctgct cccatacttt tagggaatga ttatggtgtt
     3541 gagggctatg acaggctcgg tgagtttcgc gttttagact acaatgctgc acttttgttt
     3601 tatccagggc ttaatgtcta tccgcctaaa cctgaaatgt ataaaaaaaa tcacctcgtt
     3661 ccttttactg agcattttcc ttttgaaaag ctcttcccaa agctctacca agccttgtta
     3721 aatggcgata ctcacatgtg ggaacttggt ttagagaaaa acatcttgcg cattgacgga
     3781 cttggttttg gtacgccgat ttgttttgaa gataccttcg gctatatcgg aagggatttt
     3841 gtaaaaaatg gtgcgcaagc ctttgtaaat atttctaatg acgcgtggtc aaaaagcctc
     3901 gcttgccaat accaacatct ttctatggct gtttttaggt ctgttgaaaa cagggtacca
     3961 caagttcgtt caacggcttc cggtcaaact tgttttattg acccgaatgg caggataatg
     4021 gcaatggcgg aaccctttgt tgaaacgtat ttaattggtt caataccgat tattgatact
     4081 gtaaagccaa gcttatataa taaatggggc gattacatgg gctatatttt tgttatactc
     4141 gctcttagtc tttatataat taatattagc ttaggattta aaaaggaaaa agaataatgg
     4201 caaaacaaca acaaaactca aaagcactta ctgttttggg tcctgaaact catttttcgg
     4261 gagtcttaca atttaaagat tcccttataa ttaccggaac ttttgagggc tctatagaat
     4321 cttcaggaga tctagaaata gagaaaaatg caacttgttc tgtagatacc atttatgcac
     4381 aatctattgt tgttttaggt caagttactg ggaacctcta tgctcctgaa aaaattgaaa
     4441 tgaagcaggg atgtaaagta attggaaatg tgaatactgg gaaaattcgc atagaagata
     4501 aggttgagtt ccaaggaaga gttacaatga ttgaaaaaca atctgatgcg aatctttttg
     4561 ctttaagtcc agcacaatat aaagaatctc tcaccttaat acaaaatact tgacctaatt
     4621 ttttttagag agtatactct ttatacgagt agtatcttgt gtattacttt ttctttacac
     4681 ttatcaaata gtataattca tataaataaa tcacccaact tacatggaga aaagaatggc
     4741 aattacaaaa ggccgtcgtt ttattgacac gtcagaagaa cacattgaat ctggcagtga
     4801 tgaacaaatt catttagatt taatagatga agataaagct caggaaaaaa tatctgacgg
     4861 aataacagaa gaaacaaaac ctaaggcaac aaaggtgaag gtaaacagca aggcaacaaa
     4921 gggacaagcg aaaggagctg caaaagaaaa agttgttgtc aggccaaagc ccaaaaaaca
     4981 aaatgatgta gaaaaaaagc aacagaaaga agtcgacttg catacagaag atgatttact
     5041 aaatcgtgat tcaaaacccc gactaagcat aaatgattta tctcgaatgg gaatgcacga
     5101 gctgaggaat cttgctgctg actatggact tgcaagagat gatttagcac ctatgaaaaa
     5161 acaagaactt atctttgtta ttttaaaggc acatacagaa aaaggcggta ttattttttc
     5221 ttccggtgcc ttagaaattt tacctgatgg atatggtttt ttacgttccc cgcaaaactc
     5281 ttatttacca ggctctgatg atatttatat ttccccgagt caaatacgtt tatttaatct
     5341 caaaacaggt gatactatct atggtcagat tcgctctcca aaagagggtg agcggttttt
     5401 tgccttgtta cgcattgaaa ccgtaaatat gaatgatacg agaacagcac aaacgcgcat
     5461 cccctttgag aatctcactc ccttatatcc aacagaaaaa ctaaatcttg aaacaacaac
     5521 gaatgaagtt gctacaagaa ttttaaactt gttttcaccc attggcaagg gacagcgagc
     5581 cttaattgtt gctccaccaa aggctgggaa aactatttta atgcaaaaga tagcaaatgc
     5641 gattactgca aatcatccgg aagtatattt aattgtattg cttatagatg agcgtcctga
     5701 agaagttact gaaatggagc gttctattaa agcagaggtt atttcttcaa cctttgatga
     5761 gcaagcaact cgtcacgtac aagttgccga aatggttttg gagaaggcaa aacgtttagt
     5821 cgaaaacaaa atggatgtag ttatcttcct tgactctatc acaagacttg ctcgggctta
     5881 taaccaaacg gcacctacct caggcaaggt tttatctggc ggtgttgatt caaatgcctt
     5941 gcacaagccc aagcgttttt ttggtgctgc tcgtaatgtt gaggaaggtg gaagcctcac
     6001 aattatttca acagctttaa ttgaaaccgg tagtcgcatg gacgaagtta tttttgaaga
     6061 gtttaagggg acaggtaaca acgaaataaa cctagaccga aaacttgccg atcgacgact
     6121 tttccctgct tttaatatta aacgttctgg cacaagaaaa gaagaattgt tgctgacaga
     6181 agaagaattg caaaaagttt gggttttacg gaaggttata agccctatgg acgatattga
     6241 aattatggaa ttattgcttg ataagatgaa aaaatcaaaa aacaatgaag tgtttttaaa
     6301 atcgatgaat acttcagata tgggctagat taaataagtt ttttttatta taatatactg
     6361 tactgtgatg gaggttttac atgaaagatg gtatacatcc taaatacgaa atgactaaga
     6421 ttagctgtgt ttgtggtaat gttatagaga cacgatctac tgttaaagat attaacgtgg
     6481 aaatttgctc tgcttgccat ccgtttttta ccggcaagca aaaattagtc gatactgcag
     6541 gtcgtattga aagatttaat aagcgcttta atattaaaaa ataagcctaa gatgattgaa
     6601 aagctactga aaacacattt cttcagtagc ttttttttgt ttaaattatg gagttttgac
     6661 tatcttccaa gatcgatgcg gtcttttccc caaaaaatct tctgaaatag tttgttttgt
     6721 aatttcttgc atttctttgg caaaggaaaa gttttccagc tcgactttta ggcttttact
     6781 attgctagaa aaataaaggc atccgtcggg atttaggaga gcataggttc gctttataag
     6841 ttcttcgtaa tcgcgattta tgtcgagaat tgttgtagta gattttgagt tggaaaaggt
     6901 tggcggatcc aaaataataa ggtcatattt ttttttacag ctttttaaaa actggataac
     6961 atcgcttttt ataaaggaaa aattttctcc ttcaagctta tttagtaaaa aattttcctt
     7021 tgcccattct aaataggtat ttgataagtc tacggaatca actgagcttg ctcctgcaga
     7081 ggctgcatgg acagaaaaag atgcagtata acaaaaaagg tttaagacgc ttttatcttt
     7141 tgctttattt tttactataa ggcgtaagtt tcgatgatct aaaaaaagcc ctgtgtcgat
     7201 atagtttgaa agatttattt taaacagatt atcgtgttct tctacaagta ttgttttttc
     7261 tttttccgct attttttcat attgactgag ggcttttttt ctttctcgca tttttacaaa
     7321 gatattctct tctggtatgt ctaagatttt tgcacaaatg gaaactctgt cttttatcca
     7381 catggcttct aaatcttctg tcttatctgc aggctgctta tagaaggtca agataacata
     7441 ggtttttgca tcttctttgt tttcgtagag atcgactgca aggggaaatt ctggaatatc
     7501 tttgttgtaa agacgatatg caaaaatctt attttttcga gcccattttt ttaagtgctt
     7561 atatcgtttt cgaagtctgt tttctaaaaa aactaaatta tcttgtctca ttctaagaca
     7621 cgtttttcct tatcaaatat catttgaacg ctgagcattg cataaccgga ggtaagatct
     7681 tctcgttgtg agacaactcc ttttgggagt gtgagtttta catttgtaaa attccaaatt
     7741 gcttttatac ctgtttctac aaccatgtcg gcggtttctt gtgcaaattg tgagggaacg
     7801 gtgataatag caatagctgc atttaatttt tttacctgtg tttttattga tgccatcgag
     7861 taaataggaa ccccgtgaat tttttttccg attttttctg gatcggtatc aaaggcacag
     7921 acaaattcta agccgtgaaa ttcaaattca gaatagccgg tgagggcggt tgccaagttt
     7981 ccggcgccaa cgatgatagc cttgttttta ttgtcccagc cgagaaagtg ttctatggca
     8041 gaaataaggg cgactactgg atagccgcgt ttgggtttgc caacaatgcc tgttatggca
     8101 aggtctttac gtacctgtat cggttccaac tgcaattctt gcgcgataat ggttcctgat
     8161 atatattcat agccatcgat atgcgcctgg cgaataatat gcaaataaga aggtagcctt
     8221 cgtacagaag gtgaagctgg tatttttatc attttacatc ccgtatataa ttttttcggc
     8281 aagaaccgca ttcattttat tgtttcgtgc gtcgtttaaa gccgcagttt cagtattgcc
     8341 aaaaccagta acagtaattt tggttttata gatttcttca ttggttttta aaagaacgca
     8401 aaaaatttca cattctagct ctacttcata tttgtttgtt tcgacttgtt ctggtggacg
     8461 ggcgtatttt actgtgccat agataaaata agtagaaact gctcctgtca atttttttac
     8521 ctgctgcaat aaaaactctg tattgccagc atctatttct ttataaaact caaggtttcc
     8581 aactcctgta aaaccatttc gtataagaga gcctaaaaga gcggaagtcg aatagccgtt
     8641 tgtcgttacg acgctattgt ttttatcgta gtcagcaaga cagagagagc cgcccgcatt
     8701 tattaaattg gtttttattt gaaaagctgt tttatatgct tcgctttgta ttattttttc
     8761 aatttccgca ttgttttgta aactaccttc tataactgga tatagatata gaaatgaatt
     8821 gcaggaaaaa tctgtctctt ttggtgaaaa tgaaatgaac ccttttgcat cagagataag
     8881 tatcgtctct tgaaaaaaaa tgctgtcgtt ctcttttgca atagggtagc gcagcgttat
     8941 agattggtca ataacaggat tgttttcatt atcgagtatt tgaaatataa aatctttatt
     9001 aaaagctttt ccttttattg tagcagtagg gctttgtata gtctttatag aaagatttct
     9061 tagtgtatcg ataaagtttt gcaatacaag ctgctcattt tcgatgtttt ttgtttcttg
     9121 ttctattgtc gattccaaaa ggattttatc attttccgtt agtatttttt cttttgtttc
     9181 tgtatcttgt tttaattcat ctttgcttgt ttgaaaagtt tgtttctcgg tggatagagg
     9241 caattgattt tttgggctac tcatacatga gcaaagaagc gtaagcgtaa aaataaaatt
     9301 gagaatgtat acatattttt taaaaagcat taaaaactcc agttacttat tatatagtaa
     9361 attttaaaaa ttttcaagtt ttatcagata aagatattga aagacttagt gcatcggtat
     9421 atgataaata gaatgaataa taaaattgca acaatttact tacaaaactt tcgcgacaat
     9481 atactggaga tacaaaaaca agcccttggt aaaaaaattt gtcttgctgt taaagcagat
     9541 gcctatgggc acggtgcctt gaaaatagcc aaagaggctg taaagctcgg tcttgattat
     9601 cttgctgtct cgactgtgga agaggctgtt tttttgcgcc acaatgacat tcacatacct
     9661 attttggttc ttagtattat cgatgagtca gaatacaaga atcttatctt acataatctc
     9721 tctcttgtgg ttgaaaactt tgaacaagta aaagcacttt cagcttctgc gaacgcacta
     9781 ggtaaaaaac taggggtaca tctaaaaata aataccggta tgggtcgagt tggttgcaaa
     9841 cccagcgatg tgcttcccct tgccgaatgc ataaaagaaa tggatatgct tcgtttagaa
     9901 ggtgtgtgta cgcatttatc tgtttcagat tccttgctcc cagaggacat tgcttttaca
     9961 aaaaaacaaa ttaagctctt tgaagataca ataaaaacgc tagagcaaga aggcatagag
    10021 cctggtttac gacattgtgc aaattctggt gctatttttt tgcatcctga tgcccatttt
    10081 gatatggttc gtcctggaat ctctgcatat ggctactacg caagtgttga attaaaagac
    10141 ttttgtgaac agaaaaataa ttgcttaaaa ccagttatgg agttaaagtc aaaactggta
    10201 aaaatttttc ctctttcgaa aggagattct gtttcttatg gacggacctg gattgcggct
    10261 gaagatacct ttattggact tgttcctatg ggctatgaag atgggatacg aagatgcctc
    10321 tctcctggat tggaggtaaa aataaacgag aggttttatc cgattgtagg tagaatttgc
    10381 atggaccagt tgatgcttga cttgggaaaa aatcattctt gtagggctgg agatgaagtt
    10441 attttctttg gcccaaatgg cggaaatact gcagatgacc ttgcaaaact atgcaagact
    10501 atttcgtatg aattattgtg tggaatagga aaacgcgtag aaaaaatgta tcttgactaa
    10561 ttttatagca aaagtaaaac gagaaccaat aaaaaaaggg ctacaagata gacccaaaaa
    10621 ggaagggcat cgcttcttgg atttttgttt tttgtctttt gcttactgta ggtttctttt
    10681 ggtaaggcat agccacaccg aggacagcct tgggtaaaat ctttgctctc tcctgtaaaa
    10741 ttacaagaag ggcattttac ggaagcaaaa taacgaccgc aatggggaca agctttttca
    10801 ttttgcgcga ccttagtgcc acatctttca caaaaaaagc ttggtttttt actcattata
    10861 gcatttccaa atgaagtgtt ttttgagaaa ttgagcttat ttttaacact ggctcagata
    10921 tagctttgtt ttttttgagt acagccttct gaataagatc agagggctta aggcagagaa
    10981 cactttcgtt ttgctcacaa ttaaaaacaa tgctcttgtt ttttaataag gaaacggtac
    11041 ctaaaataag ttttttggta tgaggattta tcatcaccag actttgttct atttttctta
    11101 gacttttaac atctgcaata agttttacat caaggcattt agctttttct ctttcttctt
    11161 caccaaaatt atctatctga tacaaaaaaa tttcaggttt tttttctaga aagtttccgt
    11221 tgctgtactg aacgagtgtt ttccagtgtc gaggatagtc aagggcattt acgtatattc
    11281 tttgtgggtt tatttcttcg aggttatcaa gcgcttttaa tagccatgta tatcgaataa
    11341 ttttaagtcc ttcgtcgtca agtttttgag aaagctcttt gatataatct tctgattctg
    11401 cgataatgac ggctatcatt ctgttcccaa attctccact gtataatcat aaatagacca
    11461 aatactatct ctttgtctca cgtagtaggt gtagcgtttt ttttctttga aggtattgta
    11521 gttaaaccac tgaattaccg aaacacttcc ttcagattgt gagtacattg ttttttctat
    11581 ggcaaagcgt tcgggtatag cgataatgat attatcaatg cgttcttgca ttaaatcaag
    11641 tttatagttt tcgagcattt ttattcgttc tatgtccgat aaggaggtat attttacctt
    11701 gcgttgctta tcttttgtga caaaggattc gatatcgatg tacaagaaaa attggtccca
    11761 aagacctttt tgccgtgcaa ttattgtttg ttcgacgacc ttatctggag aaatagcttg
    11821 acgttttaaa acttctgcgc tgacgatggc tgttgcgaga ctcgatgaag cagaagcttg
    11881 cggaaggggt tttacgtcta agctcagccg attggaatgt aaactcattg tttttgtttt
    11941 atacagttct gggaaaaaaa cgaggtcgat atagtagact gaggggctct ctatttctac
    12001 atagtcttta aggttttcaa caaatgagta ggcttcgcca ctttcaatgg caatttctcg
    12061 aaaataaaca gtttgattcg ttgttctttt tctgataata tcttcagttt gcttgagtgg
    12121 ctcgtttttt actgtacgtg cgttaaaatc tagactaaaa gctctgtcat cggcaagttt
    12181 aaatcgaagt gtttcaggtc ctttattttc aatagttata tacatattga ttggattatc
    12241 taccgcattt ccggtataat atacagactt attataaaag gatatagaaa tttcggcctt
    12301 cgaaaaatca tttgccccta tgctctgtgc aaaaagtaat aaaaaagtta tactaaaaaa
    12361 tagaaatcgt ttcacaatcg gttctccata accttagtat cggacaatta tgatgaatac
    12421 cttacaaaca agtacaataa atgcaatatt atctaaatca aaaagctttc aagaggcgct
    12481 ttcgcttttt tctctacctc tagagtctaa cgcatttccc tgcgaaattg aaggcttgca
    12541 aggctctcta ccgagttttt ttgtatcagc ctatgcaaag aaaaatctag aaaatcttct
    12601 gcaatcaata aaatacagtc agctcggtcg ttacacaaaa gcggatgaga tgctttcttc
    12661 tggaatccgt gatatagctt tgattgtcgc aacagaaaat gaagcacaag acctctatta
    12721 tgatataaaa acgatcttta atgatagcaa aaaggaatta agtcctgccg tctatattat
    12781 gccttggtgg gggacagtgc cgtatcgccc tgttgcacca ggttctattg tttttgggca
    12841 aagaataggc gttcttgcaa aaattgccga tccttcgcaa aaaaatcttg cagatatcag
    12901 taaaatacag acacggatat ttattattcc gcagcgaagc ttgcttattc ctgttcctcc
    12961 aaaagattct attttatcgc tttgtcttga tctgcagctt gagcaaaaaa tagacccctt
    13021 gaatattgct gagcatttta tacgttctgg ctacactcga gttcctcggg taagtgtaaa
    13081 gggcgaaatt gcgattcgcg gagaagtgct cgatattttt atgccacaag aagactatgc
    13141 gcataggatt gtatttgatt tcgatactat tacaaaaata aaatcttttg atgttgaatc
    13201 ccaggcaaca attgctgatt tagatgcgct tttgctctat ccgatgaaag aagttttgtg
    13261 ggatgaggaa aaaattgcaa aactttcaga ttattttgaa aaaaataaat ctgcctttgt
    13321 tttagatgta tacaaagaag aaggagaaaa aaaactttta caaaaaaaca ttgctgaaaa
    13381 actagatgcg tttattcagg cgctttctga gcgtggagaa gttgagggag aagaattttt
    13441 ctatcccttg gttttcgatg aaaaaacaag cgttttagat tatttttcgc cagatgcgac
    13501 tgttttttat cttgattatg atagaatgct aaatgccttg gaggggatag aaaatgagtt
    13561 ttcaggcttg tataaaagag ccttacaata cttcaacgct gaaggaaaac ttcctccgct
    13621 tccgccatcc gtactaaaac ttccttttga agatatcttc cattcttttc ataaaaagat
    13681 tctctttcga agcttacaag gtccatcttt atttgagaaa gaaaaaatcc aaattatttc
    13741 tgagccatct aaaagttttt ttggaaatat taattatcta aaggaaagtt tagaatccct
    13801 cttagccgat gactggcagg tttttatttt tgcggaaagc gaaaatcaag cccttcgtat
    13861 aaatgaattg ctaaaagatt tctctgacga gctctatatt attcctgagc cgatttcatc
    13921 tggcttttct atgcccgata taaaagttct ggttttaaaa gaaagcgaaa tatttggaag
    13981 aagaaaacaa agagctcgtt ctataaagca tgtaaaatca caagtcatag aaagctttgt
    14041 tgagctaaat cccggagact atgttgttca tgtaaactac ggcattggct tgtttgaagg
    14101 catagagcgt ataaaatcct tgggaaatga aagagattat atacgcctct tatatgctgg
    14161 tgaagaagtg ctttttatac ccattgaaca agtgaatctt gtgcagcgtt acataggaaa
    14221 tgagggaaat aaaccaaacc tcgatacgct tggttcaaaa agctgggaaa accgtaaaaa
    14281 ccgggtgaaa aaatccgtag aagatatcgc tgagcattta attggtctgt actcgaagcg
    14341 tcaagcttct cgcggttttc cgtttccaaa agatggtgag tggcaacttg cttttgaagc
    14401 agcctttccc tatgttgaaa cagatgacca aataaaagtt gcaaaagaaa taaaagagga
    14461 tatggaaaaa gcgacgccca tggacaggct tgtttgtggc gatgtaggtt atggaaaaac
    14521 cgaaatggct atgcgtgctt cttttaaagc ggttatggga ggaaaacaag ttgctttttt
    14581 ggcgccgaca acaatactcg ctgagcaaca ctatgaaacg cttctggagc gatttgaaaa
    14641 atttcccgtg aaaatagccc atatgtctcg ctttgttaca aaggctgagc aaaaaaagat
    14701 tatagagaga ttagccttgg gtgaaataga tattcttatt ggtactcatc gaataattca
    14761 aaaagatatt gtttttaaag atcttggttt aatgataatc gatgaagagc aacgttttgg
    14821 ggtaaaggat aaagaaagac tcaaggaaat gaaggcaaat atcgactctc ttgctatgac
    14881 ggcaactccc ataccgcgaa cacttcacat gagtttgcta aaaattcgcg acatgagtct
    14941 tttagaaaca gctccgcaaa ataggcaggc aatcgaaacg attatcgatc cctatagtga
    15001 cgaacgtgtt gctcaggcga ttcgccaaga agttgagcgt ggggggcagg ttttttattt
    15061 gcacaatcgt gttgaaagct tagaagaggt aaagtataag ttagaaaaaa ttctgccaga
    15121 gatgatgatc gattatgcac atggacaaat gtctcccaaa gagctggaag atatttttag
    15181 acgttttaaa atgggtggtt ttcatgtgct tttagccacg acaataatcg aaaatggcat
    15241 agatataccg aatgtaaata cgattattat agacagagcc gatatgtatg gcgtttccca
    15301 gttgtatcaa ttacgtggac gggtaggcag aagcgatcga aaggcctatg cgtatttatt
    15361 gtatcctgaa ggaaagtctt tgtctgaaat agctatgaaa cgcttgcaag ttatctcaga
    15421 ctttactgaa ctaggctcag gttttaaaat tgcgatgaag gacatggaaa ttcgcggagc
    15481 aggtaatttg cttggaaagg atcaatcggg tgatgtgtat tcggttggtt ttgacttgta
    15541 tgtgcggctt ttggaagaag cggtcaataa gcttatggac gaaaactatg aagaaagtgg
    15601 agatgttctt ttggagcttg attatagcgg ttttatacca gagacctata tagaaaatcc
    15661 cgttacaaag atggaattgt ataaaaaaat tgcttctata ggcagtcaag aagaaataga
    15721 aggcttttat atggaacttg aagaccgttt tggtcctatc cctgatgaag tatttagttt
    15781 gcttgctata gcagaaattc gcattatctg caaaaaactt tcaattgctt cgttaaaaga
    15841 aagaatggga gttttatata ttgaatttgc aaagatagca aaaatcgatg ttcaaaaagt
    15901 actttattta attcaaacct catcgggaag aattaagctc gatccaaata aggcaaatat
    15961 tctacagctt gcaacaggaa aaatcggtct aaaagaaaaa tctgcattta taagggaaaa
    16021 acttgaaaca ttattgtaat ctagaaaaat ctttttatat atggtagtat cacagtatgt
    16081 attgcattaa gacaggacaa gaattacgtg ttgcaatctc aggtaaaagc ggttgtggaa
    16141 atacaactat cagtggcttg ctggcgaaaa ccttgcatat acggcttata aattatactt
    16201 ttcgctcgct ttctgaagaa aacggtttga gtttaaatca aatcatcgag aatgcaaaaa
    16261 cagatgatag ctatgattta cgagttgata gaaggcaggt tgagcttgca aaaaaagaat
    16321 cctgtgttct aggttcgcgt ttagctattt ggatgctgtc gagtgcagat ctcaaagtct
    16381 atctcgtagc tgatgaagaa gtgagggcaa aacgtatcca agaacgcgaa ggtggttcgc
    16441 ttcaagaaat tatagatttt actcgaatgc gcgattcaga agacagagaa cgctacaagc
    16501 gcttatattc tatagacaat gacgattttt cctttgctga cttaataatc gacgtaagca
    16561 aaaaaagtcc agaagaaatt cttttagaaa ttttaaatga actagaaaaa cgctctctta
    16621 tcgattgcac accgataaaa aactcaagaa cttaagctat gattctttcc cttgatgaaa
    16681 taaaggcttt tcaaacgagt atattggagc attactatga acataatcga tcgatgcctt
    16741 ggagagaaag ctttgacccc tatgaaattt tagtttcaga gtttatgttg caacaaaccc
    16801 aagtttctag ggtgctccca aagtatctcg aatggctaga aaaattccct cgattggaaa
    16861 acctcgccca agcccctctt gcggaagttt taatcgcttg gtcaggccta ggctataatc
    16921 gaagagcaaa gtttttatgg gaaacagctc gcattattca ctttgaaaag aatggaattt
    16981 ttccgcattc tgctgcggaa ctagaaaagc ttcctggaat tggtcactat actgctcgtg
    17041 cggttctttg tttttcaaaa aaaagtcccg aagtttttat tgaaacaaat atacgatctg
    17101 tctatatttt ttattttttt aaggaaaaat ccttagtaaa agatagtgag ctttttccct
    17161 tgattgaaaa aaccctctac aaagaaaatc caaaaatctg gtactatgct ttaatggatt
    17221 atggcgcaac tttaaagaaa aaaataaaaa accccagcag aaaaagtgca catcacacaa
    17281 aacaagctcc ctttaagggc tcttttcgtg aagttcgcgg aattatccta aaagagctta
    17341 cacaagaggc agccgaaagt cttgatttaa ggcaattaca agaaaaacat aagattgaat
    17401 accagctttt atacgatgct gcagaaaaat tatgtagtga agggcttgtg ctcaaagaag
    17461 acgatgtcta tgttttagga taatatttcg ttttttctat ctagttataa gatgtatagt
    17521 atgatatact ttaaaaaaac tataaagcga ggttcttata tgaaacaatg tggtggtagt
    17581 accttatttt tggacaagac aaatattact actgaggaaa tcttttctat accagaggga
    17641 aaaacagaaa aagaagtaaa aatattcgat aatttagact taactggctt tgagtccaaa
    17701 agagatgtgc ctggtaaaaa agtgattgtt tcaagagaca attttgggat tggggaaaac
    17761 caagaatccg ttgcaaaggc tttaaaagcc gcaggaattc ttactatcgt tgtttctaca
    17821 atcgatggtg agtttaagaa aatcttaaag aacgaaaaca ttgtagtggt tgatctcgac
    17881 aaaaaaacaa tagaagatat gtttaaaaca ttttctgaaa aagatacaga ctgccttgtt
    17941 ttactttctg aagatggaac atcaaaaata aagttgattt ctggcagctt atcgaaaagt
    18001 tatacattca atttggaaga gttttataaa aaacttttac aagagggtgg cttgacgagt
    18061 tgtaagaacg tataaaaaga atttagttta atacatcgta accacatcca gcaatggctg
    18121 tggttatagc ctcttcgatt ccagcaacac tttcatcaaa atttactaaa atctgtgcct
    18181 tcatagcgtt agaaacgcaa gactgtacgc ccgcaatagc acttaccgca gtatttactt
    18241 ttgcctcaca ttcgtctcca gtcattccgg caacatatat ttttttttcc ataatcacga
    18301 cctcttagtg tttaattata cacattttgt tttatttatc aagacgtagt tttcctagct
    18361 gtccacagga accccctatc tttctccctc tttttttgcg aatagtagca tttattccgg
    18421 cattttggag tgttttcaaa accctttgta cttcattgtt tgaaggctca gaaaaaggga
    18481 gttcatcaat cgggttccaa gggataagat tgatatgtac atcaagattt ttcgcaaaat
    18541 cgataagtag cgctatctct tcagagcttg tgtttatatc tttcatcagg gcgacttcta
    18601 aactcaccct tttctttcgt ttttcaataa aatagcgagt tgcttcttta agtgcatcaa
    18661 gtttgtttgt tttagctata ggcatgatag cttctcgtgt tttggggttt gctgtagtta
    18721 atgaaatagc aaggcgaatg tctggtccat tgtcagcaag gtcataaatc ccatcaacaa
    18781 gcccggaagt tgacaaggtt attctgcgcg gagaaaggtt tctgcccttt ggctcggtta
    18841 aaaacgtaat tgtttttcga atcgcactaa gatttttcat aggctcgccc atacccataa
    18901 aaacaatatt atctaagcgc ccgcatattt tttcgaggaa aaaaaattgc tctattattt
    18961 catagctttc caaatttcgc ttaaagccaa gtgttcctgt tttgcaaaaa gcgcatttca
    19021 tagcgcaacc aacttgacaa gagacacagg ctgtttttcg tttatctgca tctatgagca
    19081 aaactgtttc aatgcaatgt ccgtcaaaga gctcaatttg caattttatt gtaccgtcag
    19141 gatcttttaa ctcttgttga atttttgacc ttgtaataaa aaaaagcgtt tcaagtcttt
    19201 cacgtaaatc aatgggaata tttgtcatct gggaaaaatc taaaacacct cgcgaaagcc
    19261 aagtataaat ttgcctggag cgaaaaggtt tttcatccgg tatgagtgca gaaatttctg
    19321 cactttctag acctaaaatt gctttttttg tcattatatt gagtgttttt atgcctgcaa
    19381 tctgtcaagc atttctttta tagcttggct tcgcattcct gttttataaa aactttgtaa
    19441 gatttcaatc gctttatttt tttggttaag tttaatatag cagtctgcaa ggtctatgta
    19501 gagtcgataa tttttcgtat ctgcttgcag gaggcgagtg aggctttcaa ttgcttcttc
    19561 atagcgacct tcacccttgc tgattaagct caagccaaga acagcaaaaa catcaaattc
    19621 gatatttaag gccttgttat agtattctgt tgctctcgca taatctccag tgttgcgata
    19681 ggcatctcca gcacgagtta aaataacctt attgtttgga tctatttcta aaattttatt
    19741 ccaatacaca atggatttat gctgttgatt cattcctcta taacaatcag ctaagccaaa
    19801 aagagcataa aaattgtttt gatctatttc aagagctttt ttgaaaaaat ctatgccttt
    19861 atcgaaggtt tttagctttc tgtggcagtt ccctatagat gtcaatatac gaatgtcgac
    19921 gagttcttcg ctttgttcca atattttggt ccaataataa agagcatctc tatattcttt
    19981 aaaatcataa tgaagatggc ctaatccgat gagagcatag gcattttgtt cttccatatc
    20041 gagaacgctt aaataaagtt cttttgattt tttaaaatcg cgtatttttc ggtaggcatc
    20101 cgctattctt gtgagaacag taatatttct attgtcatgt aacaagtatt gctcccagat
    20161 ttctatcgct ttattgtatt tattgagagc tttataacta tcggcgagtc caaaaagcgc
    20221 ataattattt cctggatggg aaataaggca ttgcgagtag taagagacag cttctttgaa
    20281 tttattttgt tttctttcgc aatcacctaa accaacaagg gcataattat tgttctcatc
    20341 tatctctaaa attcttttga atgactcctt tgccaagcgt atatcattat ctttgaggta
    20401 ttgatagcct ttttttgata attctgatat ttcttgattt tttgcgtcaa cctctgggtt
    20461 tcctgagctt gggatgtctt catttgattg cattgataaa aaatcttcca tagtgttaat
    20521 cctcagtttt tataatattt gttattagat atgcaacttt tttacaaagt ggctcatagc
    20581 ctcttttgtt gtgcgcaagc caataatatt ttaatgcttg caagtcttga ttgttttctt
    20641 tatacctttc tgcaattctc gacaaaccat cggaatagcc agtcgttata aaaattcgtt
    20701 ctgccttttg atagttgcct tcattgaaaa gaatgtttgc ttttcgattt agtaagactt
    20761 tttcttcgga actaaggctt ttattcgatt cttcatttga cttaataaac ccgccgagtg
    20821 aatctttatg atctaatgtt ttttttaaat ctttcatact ttttccgtat attttaatga
    20881 tacttcattt tttctattag tcaagctttg tagctaaaaa aatctactac agagcgtcca
    20941 aatgttcttt gatcttttct ttttaggttt tgtattgtat ctggtataaa aacttcgctc
    21001 ggtctatgta agataacaag tccattttca tgaataacag aaaaatcgga acaaagtgct
    21061 ataagctctt tgtgaaattt gtatgggaag ggtgggtcac aaaaaacgat atcgaattga
    21121 gttttgttcc gttttaaaaa aagctcagcg gagatgcact tgtatgcaat aggtttgggc
    21181 gctatttgaa tgtttttttt gatagtgtta aattttattg tatctttttc aactaagaca
    21241 acaggatttg ctccgcgtga ggatgcttca agtgctatgc ttcctgaacc tgaaaaaagg
    21301 tctaaaaaag aatactcttc gatatctccg agtattgaaa aaacggattc tttcatttta
    21361 tccattgccg ggcgtataat gccagctgga cagtcaattt ttctgcctct taaaacgcca
    21421 cctgttattc tcatgcgtct attttcctta ggataccatc tgctgccctc ataccggaag
    21481 cccaggcccc ggtgatgccg cgtgatgtgc cagctccatc gccaatgagg tagatatctt
    21541 ctgctgacat aaaatattca tttttatata cgggtttatt ggcatagagt ttaatctcgg
    21601 ggtagtacat tattgtgctc ggatgtagga gacctggaat aatagtgtca agttttttga
    21661 gagcgcgcca aatggagtcg agaaactttg agggcatagc caaagcaata tcgccaggag
    21721 tagccgattt taaggttggt tcaaagtcgt agaggtcctg gttgaggctt tctctcgtac
    21781 tacgtatgcg catacgaaaa tccccaatac gttgcataat aggattgcca ccgccggtat
    21841 gcattgcaag ggcgccgagg tattctgcat agtcttgtcc agatgcgatt ggctcggtaa
    21901 atgagacaga tctcaaaaca gcaaagttta cgagtccgtt tggttctttt gcgttggaaa
    21961 aagcatgccc gtttacagaa tagtatgttt cacccttgct tgatgtatat ttttcttgaa
    22021 caacgtgagc tgaaccagag ttcgtgcaaa aggttcttgt acgctctggg aataaaaatt
    22081 ttggatcata ataatcttca acaatagggt agtgttcttt ttttgtttca aggcggattc
    22141 ccacatcaat gctattgtct ttgtaggcta tgtctaaatg ctccatgaat tttcgtaaga
    22201 aatcaaaccc atgacgccct ggtgcaacta tgaggtgttt gtattgaatt tcgcgtttat
    22261 ctgttattgc tctatgcgct ttttcatttg ccgataaaag tgcttcgcca aaagaaagtt
    22321 cgactcccat ttttgtgagt tttgaagtaa gctcttttat aagtcggatg ccaccgtcag
    22381 ttccaaggtg tgattggcga atattgagta attgcacacc aattttttca cttcttctgg
    22441 tgtatttttc taaattgtct ttttgtagaa tatgaggatt gagaaagttt tctattcttt
    22501 caagataggg ttttgcgctt tgttttgtcc agtattcaag gggaaatcca acaggaaagg
    22561 tgaagttcat tttgcaatca tttcgaagac ctccacatga aatgggaaga ctttcgagca
    22621 aaattatttt tagttgtggc ttttgttcta agagtgaaaa agcggctccc aaaccagcgg
    22681 gaccagagcc aattatcaga caatcgcatt gaatcatagc tgaagtatag gaaattagcg
    22741 gttttttgtc taggtcattt tctatagatt gactataaga aagaattttg ttactattat
    22801 aagacccctt ggagcatctt ttttgatgtt tctaaaatcc ataaggagta atacatgaga
    22861 aaatacgaat taacgacaat cttccctgta gatgaagaat tgtacaagac ggcgcttgac
    22921 tcagtaaagc aagttctaag cgaatttggt gctgaagttg agtctgaaga atctttaggt
    22981 gatagaaacc tttattacat gataaaaaaa cgaaataagg gtcgatttct attgctcaat
    23041 attaaagtaa atcctgcaaa agttgtagaa atcgaaaaga aatttaaatt aatagattca
    23101 cttttaaaat acatgtttgt acgtgttgac gaataattga ggaggcagtt atggcagatg
    23161 taaatcaggt aacattaata ggaagactca caagagatgc tgaattaaaa tatacctcaa
    23221 atggctttgc tattgcaagt tttgccatag ctgtaaatcg aagacgtaaa aatggtgatc
    23281 aatgggtaga agaagcaagt ttttttgaca ttaatctttt tgggcgaagt gcagaaggtc
    23341 ttaagcaata tttagtaaaa ggaaaacaaa ttgctataga aggagaactt cgacaagata
    23401 gatgggagca agatggacag cctcgatcga aggttgttgt tattgcgaac actgtgcaat
    23461 tgctcggtgg taatccaggt tctagtggct cacaacaata tggtggagcc gaggggtata
    23521 gacctgagta ccaacaaaaa actgcaagct atcaagctaa aaaagaagtg caacaaaatt
    23581 cttatgagcc ctcgtatgat gactcagatt ttcctgaaga tataccattc taatttttac
    23641 tttgagtaaa aagaaattaa acctgcgacg ctgttcgcga agggagcata aatgtccgaa
    23701 aatataaaac aaaaacaaga tgaagcatac gatgtaaaga tgcaagatgt tatgaaagat
    23761 gttgatgata aatcaatgag aagtcgccct aaaacttttt ttagaaaaaa ggtttgccga
    23821 ttttgtgcac aaaaagcaaa aatcgattat aaagatccag atagcttaaa gcgttttatt
    23881 actgaacgtg gtaaaattct accaagaaga attactggaa cctgtgcaaa gcatcaaaga
    23941 agactttctc ttgagattaa aagagcaaga gcgatttgtc ttttaccctt tgtaactgaa
    24001 taaaaactga gacacatgat tagagtaaat cgatcccact cctggtaacg atttatgcta
    24061 taataaagga gcttgaaatg aaggtaatat taaataccga tgtaaaacac cttggcgaag
    24121 aaggtgatgt aaaagaagtt gcaaatggct atgcacgaaa ctttttgttt cctagaaact
    24181 tagcacttcc ctataatgac gtaacacatg agtactttga aagccgccga gcagaaattg
    24241 aagcgaaaaa agaagtaaaa cgcaatgact ctcgatctct aagagacaga cttgtgaatc
    24301 atcttgttga aattcctatg ccagctggtg caaatggaaa actctatggc gctgttacaa
    24361 gtcaaacaat ttcagatgct ttgaaaaaag ctggttttga aattgaacga aaacgaattg
    24421 aggttccagg tttaactata aaaaatcttg gaaaaacgac cgttattgtt aagctctatg
    24481 aagctttgac agctgaagtt gaagttttag ttgtaaatca agatgatgta aaagatgcaa
    24541 aagagcagaa gaaaacagaa gaaaccgctg aggtgaaagc tgaagaaaaa tctcaagctg
    24601 tagaagaaac tcctgctgaa gtggaagagc aaaacacaga aaacactgaa gcatagtgta
    24661 gagattcgta catgtcacag tctataaaat acaaagtgcc gcctcacaat ttagaagctg
    24721 agagtgcaac gcttggcgct ttgttattag actgggaagc tgttggggaa gtaattaaat
    24781 atttacgccc cgacagattt tattccttac aaaatcaaaa aatattttct ggtataattg
    24841 aactctataa caagtcgcaa cgtgcagacc tcataactct catagaacaa ctaaaatcaa
    24901 caggaagcct tgaagaggct ggcggactct cgtatatcag ttctttaaca gatacagttc
    24961 cgacaagcgc aaatgttgag cactatgcaa agattgtttt agatcaagcg gtacgtcgcg
    25021 atttgttaaa actctctgca tcgcttgcag agttttctca tgatgaaaca cagagtagcc
    25081 gttattcttt agaagaagcg caaaaagaaa tttttaactt aacggaccta aatgcaacac
    25141 atacaataaa aagctcaggt gaaatagtta atcatgcaat tgatatgatt acaaaacttt
    25201 atgaaaataa aaatgatata actggtgttc catcaggatt ttatgacttg gataaaatga
    25261 caagtggctt tcaaaattct gaattgatta taattggagc gcgcccttca attggaaaaa
    25321 cagccatcgc actttctatg gcacaaaata ttgctattga aagaaaaata cctgcagcct
    25381 ttttctctct tgaaatgtct gacacgcaaa ttgtctttcg tcttttgaca caggaatctc
    25441 gagttcgatc tgaattttta agaacgggta agctacgcca agaggattta caaaaacttt
    25501 ttgatgccgc gggaaaaatt tatgaggcgc ctctatacat tgtagactcg ccaaatatga
    25561 aacttttaga tttgcgtgca ttagcgcgaa gactcaaagc gcaacatgat gttaaaatta
    25621 tatttatcga ttatatcact ttgattacga gtgagaacac tagcttacca cggcatgagc
    25681 aaatagctga aatttctaga tctttaaaaa gtttagctcg agaattagag attcctattg
    25741 tggtactttc tcaagtaaaa cgggattcag aggggaaaca gcccagccta gccgatttga
    25801 gagaatctgg ttcaattgaa caagatgccg acgttgttat gtttttacat cgtgaaagaa
    25861 caaaggctgg tttagatgag caaaaaaatc tttctattga aacgcaactt attttagcaa
    25921 aacaacgtaa cggtcctact ggtatcgttg atattctttt ctttcccaac tataccaagt
    25981 ttgataatct tacgcgtcac gcttaattta tatttataat ctaagacttt cagaaatatt
    26041 tgcattccat tcttttattg ctttattcga ttgataccag gtgatagttc catcctgatt
    26101 taaaacaagg agtttttgtc ctattgcaag tattttttgc gggagggcct ttgtcttggg
    26161 caatatttgt gttcggtttg tttttaagtt aactacccgt acatcggatt taccaagatt
    26221 cgtatataaa atagattctt gaatagtgag aaaagcattt gtgttttcat cttgtaaacg
    26281 taagatactt gcataggtct ttcgatcttt atgataataa aataactctg ttgtaaagct
    26341 gtcattattt gaatttatac ttaagccgta aagataggga cttttgttgt ctgtatcttg
    26401 gtcgagtgca tagataaagt catttgttaa ggggagtaaa acggtctctt ttgtttttgt
    26461 atttatttct atgagcggag ttctgggatt tgttgccgca gacttggaaa taaagagact
    26521 ttcatctttt aaaagaatgt cgttaatgcc agtacctgtg tagagtgttt ccgttttttt
    26581 tgtttgaaaa ttatatgaaa gtacagtcgt gctgcttaat aaagcaatga gttcgctatc
    26641 gtatactttg aggttttcta gggattgact tggaataaat agctcttgaa atatattttc
    26701 taagacattc catgaaacaa ccgcatctct ttgtcccttt gaccaaaaat aaacactgtc
    26761 attatgaaca agtatatttt tccaattttg attatcaact attttctcta gctctttttc
    26821 attctctccg attctataga gcgcttcatt tgtaagtata tagaaactat tcgagtcctt
    26881 tgttatatcg agaaattgta cctgtgatga ttgcgtttct tttttggcaa ataaaaattt
    26941 tgcgcttgta tttagggaat agaggcttcc atcaccggtt cctataaaaa tagttcccgc
    27001 aagccatact gcatttgatg ggggaacagc aaggtcaaaa tggctttctg tgtttatctc
    27061 aaattgcgta tccccctttt ctagggaaat tttttttaag ctatagtttt ctgcggagac
    27121 ttcgtctatt aaataaaatt cttgattaga gtatcctggt attaaaacag gtttttctac
    27181 agaaatagtt ttttgtgttt ttccatttgt tgcatctatt ataaaaaatt ttttgttttt
    27241 aaatcccgca aaaaatcgtt tatttaaatc gagaaattgt cccttctcta agttactttc
    27301 agttttaatt atacttttta tattgttttc agttttaatc gtgcttttta tactgccatc
    27361 atctaatgat gcatatgtaa attgcccgtt tgaagaatag cttacgagtg ttgcttctgt
    27421 tgcgcttgtt tctgccaagc taacttgagc tatacttgta ttgagcatct gcatttgaat
    27481 gccgtatact gcgtcaagaa taattaatcc atcaaagttt ccgtttccga tgagtatata
    27541 ggaacctttt tgagaaaaag acagagaagt gattttctct tgcaaacgtt ttgcgtatat
    27601 gcgctctttt ttttgccaat cccatactga aagtctgtac acagaaaaac catcagtttc
    27661 ataaatggcg attatatttc catcaggatg tacagcgact ttttgtattt cgacatcaga
    27721 aacttgtaag cgttcttgat tcaagtttgc gtcccatcga ataacaaagc catctaccgc
    27781 acccgtataa aaaaaaccat tactttctac aatgcttgtg attagtccct tgtgtgataa
    27841 ggctaattca gattgagaaa aaagaggtat cgatgcgagg agtaaaaata aggcggataa
    27901 aaaaagtatg gttttctttt gtttgttcac aataggctcc gtgtttttaa tttttgaaaa
    27961 taaaatttat atcgccaaaa agggcaaaag caaataaaat taaaattaaa aataagccaa
    28021 caaattgtac ataatagagt atcttgggag agacttgctt gctcgatatt agttcaatag
    28081 tggcaaaaag aattaagccc ccatctaaaa tagggatagg caataagttc attatacaga
    28141 gtgaaatact tataagggcc aaaaaagaga gggcagaaat gaggccaatc gaaaagctcg
    28201 ctgcaaaacc gtctttgact gtatctccaa acataagggt aattcgtaaa ggtcctgaaa
    28261 cagcttcttt ggggtcaaga cctttaaata ataaaccaat gcctttaaag gttaaagcta
    28321 tcattttgaa ggtctctttt acaccagagg aaaatgcggt aaaaatattt tttttgggtg
    28381 tatgaacttt aaaaaattca aattgcaagc ctaggttctg gatatcattt aaaagactgc
    28441 tatctgtttc caatacaatg tttttgtttt ctcgcattac cgtaaattgt atgggctcat
    28501 ctagctcagc tacttttaag gaattaagta ctttttctaa atcactggta ttttttgttt
    28561 ctataccatt tactgcggta attatatcgt ctattttaaa tccaatactt tcggcatgac
    28621 tcccactttg tatactagta atcaaaagat ttacccagtt tacaattcct attttccctg
    28681 caccgcttga agtatctaat tctgcgagta cttgaaagtg aaatagttct ccctctctat
    28741 ccacggtaat atccaaaact ttgtttgcag aaattgctat aatgtctgat atatcagaga
    28801 aatagtgaat atttttattg ttaattgcaa gaattctatc acctgttttt aggccagctt
    28861 ctgctgctgt ggaatgaagt tctggataga cttcgttcgc tataatgatt ttatttgagc
    28921 tcgtataaaa actgtagcca atgagcgata taagagcaaa ggcaaaaata gcaaaaacga
    28981 aatttgcgag cggcccggat agcgcaataa taattcttct aagagggttt gaggcataaa
    29041 aagagccttt ttcccctcga atctcatcaa gtttattgtc gagggcaaac tgaaaatctt
    29101 tttgtccctt catcccacaa tatcccccaa tgggtataag agagagacga tattcagtgg
    29161 tctttatttt tttgcgcaat aaaacaggtc ccatgcctat ggaaaatgtt tcaacttcaa
    29221 cccctagtgc tcgtgctgca ataaaatgtc ctagttcatg tataaaaaca agtaagctca
    29281 aaccgagcaa accatagatg atactcatat caatgctcct gctttttctc gtgcaattct
    29341 atctatctgt ataacatctt ctaagtgatt tggtttcatt gaccagttcc catttagaat
    29401 tttttctgtt aagaccggaa tatctataaa gcgaattttt tcttctaaaa aagcatgaac
    29461 aaagatttcg tttgcagcat tatagactat tggcaacgca ccatcttttt ctaaaacctc
    29521 ataggcgagg gcaagcatgg gaaagtcatt catgcgagga ggagaaaaat taagcgccag
    29581 gtcctgtgaa aaatctaagc tgggtaaatt attttctttt atttctggat agttcagtgc
    29641 ataaagtata gcctgtttca tgctcggtgg tgaaagctgt gcatagtagg caccgtcttt
    29701 gctttgcacc atagagtgca caatgctttg cggatgaata agaacttgta tttcttttgc
    29761 atccaaggaa aacaatcttg acgcttctat aacttctaaa cccttgtttg caagagtcgc
    29821 agaatcaata gatatctttt ttcccatcga ccaagtaggg tgggcgaggg catcttggac
    29881 gcgaatcgaa ggaaatcttt ctttttccca caatcgaaaa ggaccgccgc ttgctgttag
    29941 gagaattttt tttacggagt ttttaccaag accttgtatg agagaaaaaa ccgcggagtg
    30001 ttctgagtct acgggaatta tttttttatt gttttttcta gcgatagatt gcaataaatc
    30061 cccggcactc acaatgcttt ctttatttgc caaggctaag tccatctttg cctcgagaca
    30121 gtgtacactc ggtaaaagtc ctgcaaaacc tgaaatgcca tttatagcta tatctgcctt
    30181 tgtttgagaa ataaattctg caaagccttc ttcattattt tctgataaaa aaagatttgt
    30241 gcaattgcat tcttttccaa aagttttaag tttggcttca tttctatgtg cacttagacc
    30301 cacaacttca aagtcgttag gatattcttt cattaaggca atgctatttt gccctattga
    30361 gcctgtacag ccgagaatga gtactttttt tttagttata gccttcattt taagctccgt
    30421 aaaacaagag aataaaataa taatagagtg gaacagtaaa aatcatggaa tcaatcgagt
    30481 ccagtaagcc gcctctgcct ggtacgataa agcctgaatc ttttaactca gcagaacgct
    30541 ttaaaataga ttcaaataaa tctcccatga ttgccatgaa ggccatgcta ctcgataaga
    30601 taagcatttt atagatagaa cctggaaaag catctgggaa aagcaatttt gcaatgaagc
    30661 cgagtagaat tgttccgagg attccaccaa caaagcccgc gatgcttttc tttttactta
    30721 cggtgaaaag tccgcgattt cctttgccaa agagcatgcc gaaaagccag gcaaatgaat
    30781 cacaaccaaa tataaaggca aggaatgcaa caaggtaaaa ttgtgaatag gggagaagag
    30841 ctatttttga aatgtatgaa aataaatacc cacagtaaaa aataataaaa aaggatgccg
    30901 agatacgttc tatagataaa acaaaaacgg aatcttcttt tttatttgta taaaaaattt
    30961 cgaaaataac taaggtaatc gttgtgataa ttaagactat attgatagta aaatcgggta
    31021 aggaaaaaat tgctgcacta tatgaaatga tgggaaaaag agaggtaagg aaaattaaaa
    31081 aagccttagc ttggagagca attttttttg aaaaaatgtc atacaattca aggcttgcta
    31141 gaatcgttac tatacaaagt gcaatatgca aaaataaatg tattttaaat ggtatataga
    31201 cgagaagaag taaaactggg actcctataa aaaaaagtag tattcgctgt aacattttgc
    31261 tcataatttt aaccttataa tgcaccgtat cttctctggc gtgttttgaa tgtttctaaa
    31321 tcgtttaaaa aacattcgtt tgtataatct ggccaaaggg tttttgtaaa aataaattct
    31381 gcataggcag actgccatag aagcatattg cttattctat actcgccacc agttcttata
    31441 agcaaatcaa catctggtag ctctggataa tctaagtagt tgtgaaaatt ttcttcattc
    31501 agtgactgta tgttctcaat tttttttatg gctcgtataa tttcatctcg agagccataa
    31561 tttatggcga gtacgacagt aagtccctgg aaatttttgc aaaaatctat ggtttcttgc
    31621 aattcttttt ggactttttt tggcaagcca tcttgccgtc ctatatgttg tactcgaatt
    31681 tgattttctt tatagaaatt aaattcttct tttaagtgag ttgttattaa attcatcaaa
    31741 taactcactt cttcttttgg tcgtttccaa ttttcagtag aaaaggcgta aaggctaagg
    31801 tagtcaactc ctgcatctct tgcagtttta actatctgtt ttgcaacatc cagacctttt
    31861 ttgtgcccag ttgttcgagg tagattttgt ttttgcgccc atctcccatt cccatccata
    31921 ataattgcta tgtgttttgg aacagaagat gaagattcgc ttgtttctgc cattagcctt
    31981 ctaatatttc cttttctttt tcttccaaga ttttgttaat ttcaaggata aactcatcag
    32041 tttctttttg aagtgcgttt tcaagtgttt ttaatgcatc ttcactgata agtccttctt
    32101 tttgttgttt tttgaaatca tcattgccat ctcggcgagt atttcgtatg gaaacacgtg
    32161 cattttcggc tgtgtttttg gcttgtttta caagctcttt tcttcgttct gcggtaagtg
    32221 gtgggattgc aatgcgaatt aactttccat catttgacgg attaaagcct aagtcagctt
    32281 tttgtatagc cttttctatt tcgcccatga gcgttttatc aaaaggttgg ataacaacca
    32341 tccgtgcttc aggtatggaa atagttgcga cttgatttaa gggggtttta tcaccatagt
    32401 attcaactcg aaccttatca aataaggccg cagatgctct acctgttcga attgtattat
    32461 actcatcttt aagagccgct atggtttttt gcattttttc agttgcgttt tttatttctt
    32521 ccattctaac tcctgtgtta taaaaataag tggactaagt cttgttaaga cctagtcctc
    32581 ttttaagttt acgcaccgag tacgtagctt acgacttttt caatgttgag ttttgcatca
    32641 acttcttttc caacagcttc aatttttctt gcaacagtca ttttatcatc ttttacaaaa
    32701 gcttgttcta aaaagcaaac ttctgcatag tgcttgttaa ttttaccgtt aataataccc
    32761 tcttttactt tttcaggttt gtcaagctct gctgtttgtt ttgtaaaaat ttccttaagc
    32821 tcatctattt cagatgcagg tacatcctca cgtcgaatat agggagggtt aaaggcagcg
    32881 acatgcaagc agcaatcata tgcgaattct tttactgttt cagagtcttt tgcagaagca
    32941 ggatcagcag aaacgatagc tacaacgcct gttttcccat cactatgaat gtaagaactg
    33001 aaaattgcat ttgcaggaat atcgatgagg gccaatctgg taattttcat attttcacga
    33061 atttttgtag ctaagcctaa aagcatattt gacaaatcat cagtaatttc tgtcatttta
    33121 ttttcaagag ctttttttgc aatattgttg cctatttcaa taaattctgc attttttgaa
    33181 acaaagtcgg tctcacaggt tacttcgcaa attacaacat ttgtctcatt gttttgaata
    33241 aaaatcttac cttcttgtgt cgcacgacca gctctttttt caactgcagc aagacccttt
    33301 tcttttaaaa gcttttcggc ttctttagca tcaccctttg tttgaacaag tgcgttttta
    33361 cattccatca tgcctgcacc ggttctttct cgtagggctt ttacatcttg tgcttttatt
    33421 tccatctatt tttttctcct gttatttata taaatcttct tcatcaacta aactttcttc
    33481 tttagattca gtttcagatt cttttttttc ttcttttaca tcttcttcag aagggctata
    33541 gtttttaaaa tctacaattt cctcttcttc tggttctttc gctacatctg tttcaatttc
    33601 ttcatcatcg ttaaggtctt caataatgcg taagcctgtt tcattatctg cttcgattac
    33661 tgcgtttgcg atgattgaga taaacaaagt tatagctcga attgcatcgt cgttcccagg
    33721 gatagggtag tcaatgcctt caggattaca gtttgtgtca acaactgcaa caatagggat
    33781 acccattttt ttagcttcgg caacagcaat agcttccttg tgtgtatcga taatgaaaat
    33841 aatgccaggc aattctttca tatctttaat gccaccaagg tttttttcga gtttttcttt
    33901 ttcttttaat atgccagaaa tttctttttt ggtaagattt gcaaaagtac catcgacttc
    33961 cattttttca agttttttta gtcttaaaat ggattttttg atagttgaaa agtttgtaag
    34021 cattcctcca agccagcggt tattgacata aaacataccg caacgttctg cttcttttgc
    34081 gattgcttgt tgtgcttgct tttttgtacc aataaataat accggtttac ctgcggaggt
    34141 aacttttcgt acagcttcat atgctgcttt aatctctaca attgtttttt gtaggtcaat
    34201 aatatgtatc ccatttcttt ctgcaaagat gtattttttc atacggggat cccaacgttt
    34261 tacttgatgt ccaaagtgta cacctgactc aagtaaattc ttcatggtta ctactgccat
    34321 gaaccactcc ttcaccggaa caaaataaat tgctttgttc ctaccattct ctatatctcg
    34381 atacaagata ttttttctgt cttatggaca agaaaataaa ttgcacggaa aatctaagaa
    34441 aaagcgaatc gcacccagca aattgccttt tctcgtacaa aaacatccta tattttttta
    34501 gtcttttata tacggatatc cttgttcttt gagtatgtca taaaactcaa aaagaggcaa
    34561 gccgacaacg gaggtgaatg agcctttaat tttttctata aaacaggagg ctaaaccttg
    34621 tatcctatag ccccccgcaa ctccgtgcca ttcacctgtg ttaatatacc aatcaatttc
    34681 tttattgctc aatttagaaa aaagcaattt tgttttgttt attcttgtag aaagataatt
    34741 gagtttacca ttaaaaaatg caatagctga aataatttct tgttcttttc cagaaaagta
    34801 ttttaaaaag ctcctagcct cttcttgatt atttggttta ccatagattt tcccatctaa
    34861 agttatcaaa gtatccgcgg ataaaatcca tggtatggtt tgtccttgtg gcatgagttt
    34921 taaggcagcg tgtatttttt ttgtggcaaa atgttctgcc gcttcctcag cattcatgcc
    34981 ttcaggaatt gtttcatcta tatcaggcat tacaacttga tagggtatgc ctagcatgtt
    35041 tagtaattcc tgccttcttg gcgaggacga agcgagtata attggttcca taaaaatcct
    35101 aataaaaatt gctaagcgtg ttttagcgcc ctgtaagtcg ttttagtaag tttaatgcct
    35161 ttgtgcgcac tggtgttaca tagttatagt ttgttgcaat tgcagagata gactgaatca
    35221 tagaggtgtt gtctgtggca gtaggtgcaa gtttttcata ggtgttaagt atttctaagg
    35281 ctaaactact tgttgggttt aaaatagcgt attttttttc tgtccaagaa atagcttcga
    35341 gtacatcatt tttgtcattt aatcctattt ctcctaatga gcgaatagca gcagttgtta
    35401 ccattggttc gttatccgca agtattactt ttaaaagaat atgtttggat tcttctgttt
    35461 ttgctttagc taaaagatca caagccttcg ctctaatgtc aggaaaatta tttgcaagtc
    35521 ttccattcgt tctaacttgc gtaaaacttc cttcaccagc aagcatttct agggcatata
    35581 tcatgtcagg gcttgtcctt ccagaatcta gagcaagttc taaatattgt agggaaacaa
    35641 gtttgctgtc tctgtcatca gctgcagcaa gctcttttac aataacatct tctacactat
    35701 ttaaatattc ttgttcgaca gttgtttcac tccgttgggc aattacactc cccagggcga
    35761 gaaatacaat taaggtacag gcagcaattt tttgtaattt catttttcct ccaaaaatct
    35821 ttacaagttg gttttaactt cattgttaag tcaaagtata gtacagactt taaaataaat
    35881 caactttata aggtatctaa ttccaatagc caagtattat taatcttaat aaaaacataa
    35941 tatactacat cttggcttcc tcgcacttgt actgctttca cgagtgtgga cgaaatgtat
    36001 cgaatttcat cgaccttact gttttgcctt gatggaataa aaatcattct aaaataatct
    36061 tcaaggtttt ttagtgtata gccctttacc ggtaatcggg aagacacgtc agttaagttt
    36121 tttttgtttt gccaatacgc ttttgaactt ggacttaaat agtataacca tttcccatag
    36181 tcctgtgcgt gcataatttg agcaagtgca tctattttct ctaaaatctc ttttttatcg
    36241 gcaaggaagg tatcttgtgt tatagattta tctttgagcc cacttgtaga gcgaacatat
    36301 tcttcatcga tttcggctgt tttttcaatc ggttttattt cttcctcgac aatgatttct
    36361 tcaagtagag gtggtaattc tagaataatt tcttcctctt tttctgctat attgctatca
    36421 tgaaaagatt gatctgtatc tgctgtttta catgcgagta tcacaaatat aagagaaaac
    36481 gtgagataca ggataacttt ctttgtgcaa tctttcatat acatagtgta ctacatctag
    36541 cttgatttcg tcaaaccttt ttctacttga cgagttttaa aaaaaactta caatagagct
    36601 atggttagtg cattatttcc aggatctttt gatccaccga cattcgggca tatgaatatc
    36661 atagaacgcg caagaaaaat ttttaatgaa attcatgtgg tgattgcggt caatagtcaa
    36721 aaaaaatacc ttttttcaga agaagaacgt tttctaatga tgaaggattt gacaaagcaa
    36781 tgggataatg tgcatgttgc tactagtaaa acgctcattg ttgattatgc aaaaaaagta
    36841 ggagcaactg ttttgcttcg tggtgtgaga aatatttctg atttttcata cgagtttgat
    36901 ttgtcaatgc tcaataaggg tttagatgca acaatagaaa ctattttttt gccaacagat
    36961 ccggaatatt ttgtgctaaa atcgagcgct atcaaagagc ttgcttttta tgatggtgat
    37021 gtttcaacaa tggttcctga gcttgtagca aaggctttaa aagaaaagta taaattataa
    37081 ataccaaaaa tagctggaaa aaagctttta ttatgacaaa atcacagaaa atgtgatttt
    37141 accatttgac aaaaaaagag atattgttat aatgattgac aagcttatcg gtattattat
    37201 atgatactta ggttataaag ataacaaatt attataatga ggtttatata tggcagtacc
    37261 aagagcgaaa acgtcaaaag ctcgcacaaa aagacgacaa cacattaaca tgaaattgca
    37321 agctcccaat ctagtagagt gcggaaattg tggcaacttg acaatgcaac accgtgtatg
    37381 tcctaaatgt ggattttatc gcggaaaaca agtaattacc ccagaagcat agggttaata
    37441 ggagacaaaa catggacgaa ttgttcacac aaatgcaaaa actcattgca aacaaacttg
    37501 aaattgaaga atcaaaaatt actttagact cttcattccg acaagactta ggtgcagaca
    37561 gccttgatac atatgagtta gtctatgcta tcgaagaaga gttaggcgta agtgtgccag
    37621 atgaaaaagc aaacgaattt gaaactgttc gtgatgcata cgatttcatt aaatcacaaa
    37681 aataattaaa aaaaaatgca agactgtgtt atgcggttct tgcatttttt ttatcttttt
    37741 atactatgcc atatacactg caaaactttc agtattcacg aaaaacacgc ttagaaagat
    37801 ttcaagagga agtagggctt tcctttcaat ctcttgattt actaaatctt gcatttatac
    37861 ataaatcata tgcaaatgaa aaaccatcct ttgcaaaaca aaaacatgtg tataatgaac
    37921 gcctcgaatt tttaggtgat gctgttttag atttaattat agctgaacat ttatatacta
    37981 ttatgctctt atctccagaa ggagacctcg cgagagcacg ctctgctatt gtctgtgaag
    38041 acagtttgtc ggaagttgca agaaaaatga aactgggctc ttgtttgctt ttaggcaaag
    38101 gagaagaggc cggtggtgga agggaaaaaa atgcccttct tgctgatacc tttgaagcgc
    38161 ttattggtgc cttatatctt gattctggtt tagaaaaaac gcggcttttt attctttctt
    38221 cgatgcaaga tcggatacgg aatgtcttaa cgcaaaaagt cggaaaagat tataagagta
    38281 cattacaaga atatgtacaa aaagaataca aaaaaactcc cgaatataaa ctacactctg
    38341 aatctggtcc tgatcacgat aagtcatttt ttattgaggt tttagtgaat gatcagagtt
    38401 ttggtcccat taaaggtaaa aataaaaaag aagcagaaca aggggttgca aagctcgcct
    38461 gtgatttttt caagcttttg taacgagcat ttatagaaac caagtaataa agcaacttgt
    38521 attttttatc atgagctatt acactgctag ttgtggtaga gcaaaaatca aaaagaaaaa
    38581 aaagaatatt tcctcaccta aaaaatagtt ctttttttct gcatattttt tattctttca
    38641 ttgtaggtgg tttaattcca gccatctttc tttcaattct tctcggtctt ttaagtgttc
    38701 aaattttatc gacggcattc aaaaatcaaa cagatgaagc tatttcgaaa gcaagcctac
    38761 ttaccgaaga attatttaag tctatagaag cgacagccct cttcattgca acacataagg
    38821 atgtccttac atgggcagaa agtgaaaaaa acgatccaaa taaaattttg gctgtcaata
    38881 agcttatttc cgaagttata gacagtccct atattcaagc atatttattg cctctagatg
    38941 gtagcgatac aatttcgcga agacaaattc cggaagaata taaacaggga ttgtatgcaa
    39001 attgggggct tttaggtgct ctttcaaaaa taaaaaccga ggggactaag cctgtttttt
    39061 atagtttgcc ccattataat atgcaaactc ccgtaagctt tgctgttggt tatcctattt
    39121 tctctaagtc cttacctata gcctatctca tacttgatgt tgaacgagat gagctctcac
    39181 aaagggtggg gcttgctgct ttttcgagcg ggtttattag cgatttattt ttagttgata
    39241 aaacaaatac gattttatac agtgtaaacg atgctataaa agaaggtagt ttttatgagc
    39301 ccaaggaaga tgggagtttt ttttctttag ataaacatct gtctcatgat ttgcgacttt
    39361 ttgcttatta tccattatat acggtgcatt attttaccca gaaaatgaag aatgcttcct
    39421 tttttgttgt atttgttagt ttttttcttt ttttaatgat ggctatattg ttatctcgat
    39481 caattgcata tccaattcaa atgcttacaa aaacaatgaa acatgtttct tatgggaact
    39541 tagatattcg ttgtgatgtg aaatttacta aaaatgcaga aatgtcattt ctcatagaga
    39601 gttataatta cagtttagat caaatacggg aattgtacca caatagaatt gcacaagaga
    39661 gaagtttgcg gcatgcccaa gtacaagcat tgcaagcgca aataaacccg cattttttaa
    39721 ataatacctt aaattcaata aaatctatgg cgttacttga aggcaataaa gatgttgcag
    39781 atatgattac aagtttggct cgtattttta ggaaatctat ttctatttct tcggaattta
    39841 caaatcttgc agatagtata gcaatagcaa gggattattt tacgatagaa gcaaaacgtt
    39901 ggccaggccg ttttactctt actgaaaata ttgaaggaga gctctacgat gccgttttac
    39961 cccacttggt attacagcca atttttgaaa atgctttgat gcatggttta gaaaaaaaag
    40021 agggtaaagg aactatttgg ctacgaattt ggctctgtgg tggtgattta tttatagaag
    40081 taaaagacga tggtatcggt ataaaaaaag agaatttaaa aaaaatacaa aaaaaactgg
    40141 atgaagctcg attacatcca gaacttataa gtgatttgca aacaaaaaca aatatgtctg
    40201 gtagctctgg aattgctttg ataaataccc atagacgttt atcgctcatc tttggcaaaa
    40261 catacggtct tcaaatagaa tcgcaagaaa attttggaac aaaggttttg atctgtttgc
    40321 cgtataaaaa ggagggttca cttgtttaaa gttcttgttg ttgaagatga atctcttgtg
    40381 cgccgcgctt tagtttctct taccccctgg caggatttag gctttgagtt ttgtgctgag
    40441 gcagaaaatg gttctgctgc cttatcatta tttatgcaac atcaaccgca agtgataatt
    40501 actgatatac gaatgcctgt aatggacggt ttagatttta tacaaaaggt ttttgattat
    40561 gtgcagaaag aggcttgtcc agagcctgag gtaatcattt tgacgggcta ttctgaattt
    40621 gagtattcaa gaaaagcctt aatgcttggt gtgcatgaat atgtgcttaa accagtggaa
    40681 gaagaaattt tatataattc tttagagcgc gctaagcaac gtcttttaga tcgccaaaaa
    40741 gaaaaagaaa gagtacaaat ctctcttttt gaaaaaatac agtctgcgga taacgatgat
    40801 acggggatag cttttaatta tgtaaaagaa agtattgcgc tcatagaaaa ccactatgtt
    40861 ttaggtatta ctatagaaga agcagcccag aaactaggca tttcatcagg gcatttgagc
    40921 cgacttttta aacaaagcac caaacttaca tttaatgaat atgtacgaaa tgtacgtatt
    40981 gaaaaagcga ttgctttatt aaaagatcag agtattaaga tttatgagat tgctgactta
    41041 gtcggatatt tagatgtaaa atactttact caaacattta aaaaaattac gggtttaact
    41101 ccgagtgagt atcgataccg tttttttcaa gcaaatcaag ccattgatac agaataagct
    41161 ctcgtgaatt tgcagcatca gtagtggggt attgaatgag gggactcatt atttttttat
    41221 tgcttttttc caattccgtt ctaaccgatc ttcgttgcca acgaggtgaa agaagttttt
    41281 gaacatcttc atctaggaca aaatcgatga aagcttttgc ttctatgaga ttttctgcat
    41341 tttttaaaag cgcaactcca tcggggagaa gacttgtacc ttcacttggg tatatatagg
    41401 ctacattgga actgagcttt tctaaagtga ttgcataatc ttcaaagctt atgcctgcaa
    41461 agcaatcacc tgttgaaact gctttaagaa tagtatttga attctgtgta ataccttctt
    41521 ctccaagctg tcctatgagt gcattcaaaa aatctctacc gccaaaaaga acatttttgt
    41581 ttgtgttttt cacatataag agggttgtta gaatggtaaa ggcagaacca gatttttgag
    41641 ggtcagccat aatgattcgg tttttgaaaa agggattcag caaatcatcc caagattttg
    41701 gccataaatc aggaggaata agacttttgt tataaatgat aaccatcggc agaacggagt
    41761 agggatgcca aagagaatgg ggcgatttat acgtcggata tattgcattt gttacctcac
    41821 tttcataaaa caaaaagtga tcttttattg tttctaatga gtctatgcca ccaccccaaa
    41881 agacatctgc ttttagatag ggaaggtttg caaccttttg cataagttcc cctgttcctt
    41941 cacgaataac agtgattttt attcctgttc tttgatggaa ctcttttgtt ataaaatcta
    42001 tcatatcttc agggtgagga gacagtataa taagttcatt tgtttttttt aatggtttat
    42061 tgctatcctg ttttgcacaa gatagcaata aaatgaatag aaacatacat gagatgatag
    42121 atatcgtttt tttattcttt ccccgcagca taggttgaag aacctccaac aaaatattta
    42181 gaaagactta agaaaagagc tataacaggt atagaagcaa taacggcaac tgccatcatt
    42241 gcaccttcat ctgcatattt ttcagctgca aacttgacaa tataggcagg tattgttttc
    42301 atctcttctc tttgcgttac aagtagtggc cacaatagtg tatcccattg ttgtcgaaat
    42361 gtcaaaaccg caactgtcgc aattacaggt gttgcatttg gtaaaataat cttggcgtag
    42421 atagacggtt cattcattcc atctatacgt gctgcatcta aaatatcttc tggaaaggta
    42481 agtaagtatt ggcgcatttg aaaaacagca aaggcattaa gcataaaagg aataataagt
    42541 ccacgatagg aatcttgtat gccaagtttt agcgctacca taaataaggg aatcataata
    42601 gtttcaaaag gtatcatcat tgtagccata attgcaagga aaacaatgtt tcgcccttta
    42661 aaattaaatt tggctaagcc atatccaacc atggaagata aaaaaatagt tgataccata
    42721 atagaaatag aaacaattaa gctgtttgca atgtttctaa caaattgata acttccatca
    42781 ttacctgcaa ttgctcgagc atagttggcc cattttattt ctgaaggaat ccaccgataa
    42841 ggcatggtga taatttcttt tgcatccata atgcttgcgg taaacataaa aacaagcggt
    42901 agtaagatta atatggcaaa ggttaataaa aacaaccaaa tcagagtgtt aaaagttgtt
    42961 gcgagcgttt tcatttgatt cttattaact gttgccggca tggctaaaga attcatagaa
    43021 gttctcctta taattaaaac gaaacatcat cacttttact tgttttgaat tgtactattg
    43081 taagtaaaag catgattaag aataaaataa cactcataac acttgcgcgg ctgaggttat
    43141 tgtctcgcat cgctgtatta taaatgttca gggtaatgac attgataggg gcactcgggg
    43201 caccgctttg tgtaaataaa tactgcgtgc taaaggtttt taaacattga atcatagcca
    43261 taatcgaaac catcaaagtg gttggtttta agagtggtat ggtaatgagt ttaaattgat
    43321 tccaaccatt tgccccatca attgtagcag cttcataaat agccgttggg atttttccta
    43381 tgccagttat atagagaatg gcaaaatagc caatatattt ccaaaaatat actataatag
    43441 tcgaaagacg caccattgga ccacttgcaa gccatttata atcaacacca cttgttgaaa
    43501 aaagagtgtt tagtgtttga tttaacaagc cagttggttg tagcattaaa aaccaaataa
    43561 gtgctgcaac tactgatgac aaaacagctg gtgagtagta gacaagctgc aagcttcgat
    43621 taaatctttt tctagaatgt ataaaaatag ctaaaacaag accaatgatt acaagaggga
    43681 taaaacaacc gactgtaaaa acgagagttg ccttcataga gttccaaaaa tcataatttg
    43741 ttaaaatttt tatataattt gtgagcccaa caaaatttgc ttttttaagt gaaacaagtt
    43801 ttttgttgag caaacttgtc caaaaagcat ttaaaatagg gaaaaaggaa aaaatagaaa
    43861 aaaatacaat tccagggctg acaaaaatcc atccccatcg tgcttttttc tgttcatagc
    43921 taggtttagc ttttaatttc ataatatctc caaaacgatt agacttaaaa agtcagcgta
    43981 gattctacgc tgactctcat atacttagtt atcaagtact tcttgtacct ttgtttgtaa
    44041 ggtatcaacc gctcttgctg gagctacttt gttaagcata acagattcga tagcttcttt
    44101 aataaggtca tttatttgtt gtgaagcttc tccataataa actactggtg caatttctaa
    44161 ttcatctttg aatacctttg agaaaggcat gtttttgaac gttgcagagt tgaaaagtgt
    44221 gtttgtaggt tgtacaagag caactttttc taagtattct tctccatggc tgagcataaa
    44281 ggatacgagt ttccaagcag cttgttgtcg ttctttagat acttggctgt ttaccatgta
    44341 atagtgcccg tagtaagtgt taggaacttg ttttccacca gcgaaggttg ggtaaggtat
    44401 aaccatccaa tcatcagaat tgaaaaagtc gggattagcg cttgccattc tttgttcttg
    44461 atataaacca gagaggtgca tagcaatttc attattatcg ttatcaaaaa cttttcgtgc
    44521 tgcagtatag gttggggagc caaggttttt accgttaggt ccaaagtctg ccatgtattg
    44581 cagtactttt acccatgcat ctttgtttac gattgcagtt ttgccatcgt cagaaataag
    44641 ttttccaccg agttgttcaa ccattggaag ccagctaatt aaatagtcac cgtatctgaa
    44701 gtcaaaacca cggcgagtga ttatacttcc gtctcgtttg acaatttttt ctgaaacagt
    44761 cattacttct tcccaggttt ttggatagtc tttgttcgca tctaaaccag catcgcggaa
    44821 gattttcttg tttagataaa ttgcccagtt tgtcaattcc aaaggaagac catagacttt
    44881 tccatctacg gtaacaggat ctaacatacc tgccatataa gaattgagta tttcgttgtg
    44941 gcttttatag ccagctgatg caaggtctac aggtgcaaca aaaccttctg ctaggaacgg
    45001 ataggctttt tgaatttcaa ggttaaaaat gtctggccca ttttttgcag cgaatccagc
    45061 ttgaataact ttgtggattt ctgttgaggg gtagacaaca tattttactt tgatattggg
    45121 gttttgtgct tcgaattctt tgatgtattt ttcttcaatc gcttttcgat tcacatcttc
    45181 gtgtgtccat attgttaatt caataggacc tgttgctttg gcatcttttg tgccagcggc
    45241 aaacaatact aagggacttg caataatgca tagcattagt acaaacatca tttttttcat
    45301 aatacctcct aatatattaa gtttgcaacg caaacttacg ttgaccacct cgacgctatt
    45361 ttagttgagg tggttaacaa acttcctaaa aaagtcaaag agaaagcttc agctttcgag
    45421 attgactttt ttctttctag ttattatttt catccgactt tcattagaaa acaaggagag
    45481 tatgcataca aaaggtgtgt aattctgata tttgtgagta agattagtgt ttatactaag
    45541 attttagcga agtcgtcaat caatataagt tctttttctg tgggtgtttc aaatttttca
    45601 ttaaaaaata aaagcttttg gtcactaaaa taaaaactgg cttcgggcat ttttttttgt
    45661 cttttatcga ggtggcttct ttgttttata tcatctatcg gaaaatacag gataatgcgt
    45721 ttagaatttg ggttcatttt ctcaaaataa tcaaggcatt tttttctctc ttttgctgtc
    45781 caaaaaccaa aatcaagtac cacgtttata tctttatgaa gtaaatctga tgctatttta
    45841 tacatgagat cggtacattt ttctaatcgt tcatgaaaga cagagcaatc ggggctctct
    45901 ccataaaagt gtatcatcca ttcgtcagta ttgaatacaa aatagttttt tttatttttt
    45961 acttgtgagc aaaaatgtgt ttttcctgaa gctatttttc cgcagcaaag aatgagtttt
    46021 gacataattt ttcctttttt tatcatacta tattttttca aaaatgacag gcggataatg
    46081 taaaaactca gaacttatgc tatactattg ctattatttt ctaattaaaa aataataatt
    46141 acttttagag gagtacctat ggcatttcca aaaaatttta tctggggggc ggcaacttcg
    46201 tcctatcaaa tcgaaggagc atataaggaa gacggtagag gattatcaat atgggacgct
    46261 tttagtaaaa gcccagggcg agtattcaat ggagatacag gagatatcgc ctgtgatcac
    46321 tacaatcgtt acaaagaaga tgtcgcttta atgaaaaaga taggtcttga agcctatcgt
    46381 ttttctatag cttggtcaag aatttttccg gaaggcagtg gaagagttaa tgaaaaggga
    46441 ctcgattttt attctcgttt agtagatgaa cttctagaag caggcattag tccctgggcg
    46501 actttgtatc attgggatat gcctttacaa acctattata agggcggctg gcttaataga
    46561 gaaacaacag atgattttgc aaaatatact gaagtgattg taaaaaagtt aggtgatagg
    46621 ataaaaaatt ggatgacctt caatgagccg cagtgtatta ttggcttggg gtatgaaaca
    46681 ggcgaacatg ctcccggtct aaagtcaagc gattacgaaa ttgccactgc tttacataat
    46741 catctcattg ctcatggaaa ggctgttgat gtcttgcgtt cctatggtgg cgatgccttt
    46801 tctattggct atgttcctac ctgccaaaca acaattccaa aagattctag cccagaagct
    46861 atagatattg ctcgtgaggc acttttttct ttacaagact ttagatcgcc tatatggacg
    46921 atatctacaa cttgcgatcc tgtatataag ggagaatatc ctcaagatgt attaaaacga
    46981 attgaagata aattacccaa gggctgggaa aaagatttag aaaacgtagg tggaaaaaca
    47041 gattttcttg gtgtgaattt atattttggt tacgtagtag ataaagtaga aggaaaaatt
    47101 caaatcgtaa aaccaaaacc aggtatggct cagactgccc tgcgatgggg tgttgaagaa
    47161 gaaacgcttc attgggcgcc gcggttttta tatgagcgct ataaaaaacc ggttgttata
    47221 actgaaaatg gcttgagcaa cacagattgg aaacaatcag atggacgagt gaacgatcct
    47281 gcacgtatcg actttgtaaa acgatactta gttggtttac acaaggcaat tgaaaacggc
    47341 gcagatattg cggggtattt tcactggagt ttgatggaca attttgaatg ggctcaaggc
    47401 tatagagatc gttttggaat gatttatgtc gattttgaaa cacaagagcg tattattaaa
    47461 gaatctggac actggtatag gaaagttatt gaatcaaatg gtgaattgat cttataaata
    47521 atagacattt agttttataa ctaatgaaaa aatccctgta gagtgaaaac ctaccaggga
    47581 ttttttttta atactgcggt ctatccattt tatcccttac ctctttaaga agttgtgcac
    47641 ggatattttt atctagttca atatgatata tctggtcaaa ataataggcc ttatgaatag
    47701 tttctaagac tgccaataca atatcatttt caaaagcctt tgatccgagt aaaaatgctt
    47761 ttgacgaaag gctaattttt tcatagggaa atacttttgc ttcttgaaag aaaaaagggc
    47821 ttgttatgtt ttcgataaaa ctaaggctgt cgctatgaaa gataaggcag tgaaattgtg
    47881 tagtaaagct ttttttctgg ggaaaaagga ttacgagaga tgttttattt tcaagcttac
    47941 tttcaatgcg tttttttata tcaaatattt ttttactttt ttttgtatag agctcatcgt
    48001 aggcttcatc caaaaataaa aaactcgtat gatttacctc ttctatctct atcctttgtg
    48061 gattattttt tgtaaggtcc ttaaaggtat ttaaaatata gctttttctg ccattctcat
    48121 cgagtgaaga aaataattct tttgatataa ttataccgct ttctattatg tcgttttcgt
    48181 attcttgtat ttcgcttgga cttagtttta agagtgtttt gtttttgaga aataaggctt
    48241 ttaaactttg aatagtaagg gtatctttat tatttcgaga aatcgtttcc atttctttga
    48301 gagcctttga cctattttct gggctgttaa aatgcttgag ttgatgtaag tgcgcttgta
    48361 aaaataaaag cgcgttaaaa ttctcatagg cttccatggg cacgggattg tactgataaa
    48421 aaactgctgc gtcaaacaga gcttgtaaaa gctgtattgc cttttcttct ttgtgcttcc
    48481 attctttcag ttgacgtaat tgtaagtcga gacgagtgta tttttcttct tctagatatt
    48541 ctagcgcttg gtcatagagg cttgaaaatt gtacatactc gtttaatgtt tttttctcat
    48601 cttgcccaaa agatttgtac tcgatggaca tggcgagata tgcaagcgga aaactataaa
    48661 aggcatttct ttcatcgatg ttcattccct ttggttctat gtaggatata gagccatctt
    48721 ggaaaagagt aatcgccttt tcatagccat cttctccggc aaataaaaaa gtcttacttg
    48781 tattgtcatc gaggggattg agccaagatg cgatgagttt tgccttgtct tgttttagta
    48841 ggtttttatt tttaaatgta gcataggata gcgagggaag ttttgcaaag ttttttacca
    48901 agccaaaacg aagagcgtcg atttggctat cgtctcgcgt aaagtatttt tcgttttcaa
    48961 tttcattttc gattttgttt ttttgattat tttcttgaag aagattagaa tcaaaatggt
    49021 caaaaaaatc tttttgaact ttaaattctg caggaaaaaa atgtgcatcg attctctgca
    49081 ttgtattttc aagagcttct ctaatttctt tttcatttac aaaaaagatt ttttcaggat
    49141 agaggtcttc tatatttttt tttgtgcgag aaataaatga gtctgtaaaa taattatttt
    49201 ctaaagcttc atctattgct ttttgttcaa aggctttttt tataggacta attattgtat
    49261 tgtaagaaaa atctgagcta tgtattgtgt ttactatttc taaaatagag tcagtatctg
    49321 caagttcgga ttgtagtatt tcgggcactg gaagatagca gtcttgtgta tcttgatttt
    49381 tttcaaatag gattttattt ccatctatgc ccatgagaac ttcataggga taaatggcaa
    49441 ggtagactcc gtcttttgtt tcaagtttta aaagaaaatc tatattgaag gaggcgcttt
    49501 gtaaaaaaag actttctttt tcatcgatga tacgagtgct cgtttttgaa aacctttcta
    49561 tcgtttttcc caaaaaaggg atttttcgaa aatcaatttg caagaaaaaa aagaaaagac
    49621 ttgctgctgc aagagaaaag aggaggattc ctagaatgcc ttggacgagt ggatgcattt
    49681 ttttagtttt ctttttcatt atcaaaatct cctgcatcat tttcttgtgt ctgtatagaa
    49741 cttgtggtac tttccttatt ttctgggtag tgaaaactga gctgtatttc cttccaacca
    49801 aagcgctgaa ataatcgctc gcttgccctt agaatagctt tttcttgttc cgctcttttt
    49861 gtattttccc agtgaaataa atcttctttt gtatgaataa gatttgcgcc ttccaaaagt
    49921 tccaaggcaa tgagtccttt tgaagcttgt aagatatgct ggacttcagt aaaaccgtag
    49981 cctgtcgctt cgtagtcgag caaaaaattc ttcgtacaaa gatagctcgt tatgcctgtt
    50041 gcaagggcgg caagccctgc ccctatcgaa gcgcctgtta gttttgtgat gggctttggt
    50101 aagagtcccc cgtctaaaag cgaggcagct attcccgcac ttgttgagcc gacgatgccg
    50161 cctgcaaaac cgccaagtcc cgagccaact attgcgagag tttttgctat ctgttttgct
    50221 tcatcttcgg tatagggttt tggttctatg ctttcaactt cttgtatacc tgcttcagga
    50281 atagaaatag aaacaaagac aggcataaga ctttctgagt taaaatctgc atagagtttt
    50341 ccatttttag gattacttcg ctctgtatcg atttttattt ttgagaggtc gaagccaaag
    50401 cttgcagtcc ccttgacgag gtagacgatg agtttgtcgc tatggacatt atctgtcttt
    50461 gtgccatata caatgtattt ataaaagtcg acgcttttat aaaccaatac tgggctttca
    50521 ttatggagtg gaatttgctc cctgtactgg ttaaatgctt caaaacttgc gtccttcgtt
    50581 tgttttttgt aggcaaggta aaataaaagc ggagagatga taataagggc aaagacaaaa
    50641 aaagcaattt tagttttcac tcttttatag tatcctatgt ttagaaataa ataaagagaa
    50701 aaatctgtat gtaaagaaaa gctttaggaa agaagcatgc ggtcattaat gattgcatct
    50761 tgtgctgagc tttgaaactt ctgcataagg tcggcaactt ctagttttct tttttcttca
    50821 ccccttacat cgtagatgat ttttccagca ctcatcatta tcaatcgatt accatgtcga
    50881 attgcatctt tcatattgtg cgttaccata aaggttgtaa gtccttctct tttcactatc
    50941 tcatcggtta gctctaaaac cttttgtgcg gttttggggt caagcgctgc ggtatgctca
    51001 tcgaggagga ggaggcgggg tttttgtatc gtagccataa gaagggtaag cgcctgtctt
    51061 tgtccgccag agagtagacc aacctttgtt gtcatcctgc tttctaagcc aaggtcaagc
    51121 attgcaagct cttttttata gcgttctttt tctgctggac gaattcccca ggcgaggctg
    51181 cgccttttcc cccgccgata tgcgagggcg agattttcat cgatttgcat atttgcagca
    51241 gttcccaaca tcgggtcttg gaagacgcgg ccgagaaagc gtgctctttt atgttctttt
    51301 tcttcggtaa catcgatgcc gtcaaaataa atatgtcctg agtcaacttc atagactcct
    51361 gcgatgaggt ttaataatgt cgattttccc gcaccattgc cgccgatgac gcttacaaag
    51421 tcgccttctt ctagtttgag gtttatatcg attaaggctt tgcgctcgtt aattgtgcct
    51481 tcattgaagg ttttatgtac agtatctaat gctatcattg tatactcgct tttttattcg
    51541 cttctgtttt tttctgaagg cgatttattt tacgtttttg gtgtcgggtc attttttcat
    51601 tgataacggg aagggcgagt gcaagagcaa cgatgactgc agttaaaagc tttaggtcag
    51661 ttgatttcat accgagttgt aaaacaattg caatgataat gcgatataaa atagagccaa
    51721 agacaaccga cataagtcta taccaaaagc taaagcgttt gccaaaaata acttcaccga
    51781 tgataatcga agcgagtcct ataacaattg ttccggttcc cattccaaca tctgcgtagc
    51841 cctggctttg agcaaccaag gcaccgctaa aggcaacgag ggcatttgaa agcatgagtc
    51901 ctatgttttt catcgattca gtattaatcc caagcgcctg caccatcttt tcgttacttc
    51961 cggttgcacg aattgcgctg ccaagttctg ttccaaaaaa ccagtacaag attgcaatta
    52021 caattgcaga aaaaatactt cctataagta gggaaccgag ggttttactt aaaccaaatg
    52081 aagaggtaag agtgagcatt gtttcttgcc caagaagcgg tatatttgcc tgtcccataa
    52141 ctcggatatt taccgagtag agtgcaatca ttgtaagaat tcctgcaagt atgccgggga
    52201 tttttagttt tgtgtgtaaa agccccgtaa cgagaccagc aagagagcca gcaaaaaaag
    52261 caatgactaa gctgaggtag ggagagaaac cgcttgtgat gagaattgca gaaacggagc
    52321 caccgagagc aaagcttcca tcgaccgtga gatcagcaaa gtttaaaatt ttatacgtaa
    52381 taaaaagtcc cagagtcata agcccccaaa gtactccttg ggaggcggct ccttgtatcg
    52441 caagaaaaat gcccatgtat aaatccttat ttgcctagtt ctaaaaggag ctcttctgga
    52501 atgataatcc caatttcttt tgcgcttgct tcatttagag caaaatctcc accttgtaag
    52561 tattgaatag gcatagttgc tggttttttt ccgtcacgta gaatttctgc agccatcttt
    52621 cccgtttgtt ttccgagtgc gtagtagttt atgccatagg ttgcaagacc tcctgcttgt
    52681 accatacctt cttccccaac aatgaatgga atttttgcag gattcagtac accagcaaca
    52741 gttggaatac ctgcagctat catattgtca gttggtgagt aaactgcatc aaccttacca
    52801 acaaggcttt gtactacttg ttgaatttca tttgatgaag aaacagttgc gtcgagatat
    52861 gaaataccga gcgcatcggc ctcttttttt gctaaatcaa tttggaattt tgagttttgc
    52921 tcgcttgaat tgtacaaaaa accaattgtt tttacttcag gtacgagttt ttttatcaag
    52981 gcaatttgtg cagcaactgg agttaagtca gaggttcctg taacatttgt cccaggcatt
    53041 tcatttgatt taacaagcat tgcagattga ggatcggtaa ccgcggtaat gagaataggt
    53101 attgtctctg taaggtttgc aacagcttgt gctgctggag ttgcaatggc taagataaga
    53161 tcgcttttat cgttaataaa tttttgtgcg atggtttggc aatttgcttg ctcaccttgg
    53221 gcattttgaa agtcaatttt gattgactct ccgtcaatat aaccttcttc agcaagagcg
    53281 tcgacaaaac ctgcgtaggc agcatcgagg gcgacgtgtt ctacaagttg tagggctcca
    53341 atttttattt tatcgtcttt tttgtttgca caagacaaaa aaaatccact catgctcaca
    53401 acgataaaac tgagaaaaag aattctaaat acatttcttt tcataattcc tcctacgttt
    53461 agataaaaag cctaatatca ctaagcctct gtatcttttt ttcccttttt ttagtagcta
    53521 aatactaggg aatgggaggt aaaatgtcaa tatgtagaaa aaaattatgg attccatgcc
    53581 tcttttccca agagcatttc tacagtgagt accttgcttt gatgttcagc taaaagtgca
    53641 taggcctcgg ctaagtcgcc aagtgataaa atcattgttt ctataaagtc agcttctgaa
    53701 ataataccca tttccatttg tgttttttta tcgagcaatt cacgattgtt tacagcttgc
    53761 ttgagttggt aatattcaat ttttgtttcc aataacgaaa gtgataaata gctttctcga
    53821 atatgtgcgc tcagtatttc tttgccctct t
//
LOCUS       DAOSSW010000003        47940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_842_length_47940_cov_7.017814, whole genome shotgun sequence.
ACCESSION   DAOSSW010000003 DAOSSW010000000
VERSION     DAOSSW010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 47940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47940
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_842_length_47940_cov_7.017814"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /gene="metK"
                     /locus_tag="PKW26_00545"
     CDS             <1..835
                     /gene="metK"
                     /locus_tag="PKW26_00545"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOQ92086.1"
                     /translation="GLKEFEGQQGAGDQGMMFGFACKETPELMPAPLMYAHSILRKAS
                     ELRKTKTIPWLRPDSKSQVTIAYEGFTPVRIDSVVVSHQHDPDVPYETIKETIIESII
                     KPTLESSALLDKETKFYINPTGRFVIGGPFGDTGLTGRKLIVDSYGGMGRHGGGAFSG
                     KDPSKVDRSGAYMARYIAKHVVAADLAERCEVQLAYAIGVPYPVSIFVETFGTGKVEE
                     LAISKAIEEVFDCSPAGIIKKLDLKKPIYKKTASYGHFGRDDFSWEKLDLVDALKKAI
                     K"
     gene            932..1567
                     /gene="nth"
                     /locus_tag="PKW26_00550"
     CDS             932..1567
                     /gene="nth"
                     /locus_tag="PKW26_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOQ92087.1"
                     /translation="MVLQDERTIFEIFSLFQKANPEPKTELLAPNAFTLLVSVVLSAQ
                     ATDRSVNKATEALYKKVQTPEAMLALGEEGLKEHIKTIGLYRVKAGYVIRLSKILVED
                     FQSKIPLNREDLESLPGVGRKTASVILNVLAGEATMPVDTHLLRISPRIGLSDGKTPL
                     QVEKDLLERIPKEFLQHAHHWLILHGRYVCIARNPKCESCLISHLCKKNCL"
     gene            complement(1671..4184)
                     /locus_tag="PKW26_00555"
     CDS             complement(1671..4184)
                     /locus_tag="PKW26_00555"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92088.1"
                     /translation="MTKYGSFNEKNEFVIHNPYTPEAWLQYLIRPEQKSTSTFCSGVT
                     HAGGGFDIQGTHENTLSDTKIHLQEEDSLVRCIYIKDKKTNEIWSTSWQPTRKEEQKF
                     TTTFSFGKILFESSYKGIDTKVSMFVPKIFDGWVQNVSITNTTNEEKELELYPFVPMH
                     IGDALQRLMAGDNDAFFGGVKYDADLKGLVFRRHHGIEVNDDPEKINGMLGTTVLFHS
                     SLNKENQAYESDLLHFYGDRFHNHTNPKTILESKLSNRDIPHLRRPCGVFQHIVSLKP
                     GETIDFALSLISDKTSDYYLNNKTELKAHLEAISTKEKREALLADIEEWWRKRLESLQ
                     IKSPNKDVNTSFQWLQYQCEIVLILNRMKSRFHTGYEYGWGFRDILQDILYLLPYRPE
                     LMRELLLHISTQIFTTGISYHNFFISQPGNKSIEASDDPLWFVQAVIQYCYETGDYDF
                     LNEVSYYAEKREDNTEAKGTILEHCFVSLDRVITDRSERNLPYLKDCDWNDDLNEKRV
                     NDEPSKNMESIMVAQQLVGALHEFQKLMKNKLPQNAEIEKKLSDYAKAENETREALLK
                     YAVDKKGYFKRALSVKDASEDLGSSENLEAKIFLEPQIFAITNETVDLEKGKELLAHV
                     EKELDSPWGAMLCYPYFNGLAYRDELPQRTWNIEKEPPGLKENGSIFMHLNAWLVEAY
                     CKTGQGNKALAMYEKTLPMNLSKDQDSYSCEPYVYPEYVRGKGAQEEGRGGHTWLTGT
                     APTMHKTIIESIFGVKPVLEGLRIKPCVSSEWKAFSIKRKYQNAVYSIDFENPEGVET
                     GIARVTLDGKEIQSEKDSVILPSFGDNAEHYVIISMGAN"
     gene            complement(4162..6246)
                     /locus_tag="PKW26_00560"
     CDS             complement(4162..6246)
                     /locus_tag="PKW26_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92089.1"
                     /translation="MAQCIFINNNFEEIDPFGYEIESFVKKPQLALQVKEFLKSSYVR
                     SYPLKVLILFEKFVSLVDSQENPSKKILKELFLQSRFSFMGTDGMRGKIATEKSQNPI
                     LDFTQNLILSPELLYLSSKAFIKMLSDNGTITKRSNVCTGNDGRDIATNWALTNAMNH
                     GLENFGHKVFDLGIVPTPAVPYQMLIDGIDAGAMLTASHNPADQNGIKFFIKSKKLLP
                     EGKNGDYELSAYMFLERSSINTSIEIRQASHATKKEIDFTRFLDERLPLNIGEALNAI
                     DLYLDNANGAWAEIAQYYFAKQKIAFASPNEKPIGTNINKQCGVAEIEGHSLYTKKDY
                     TSAPAIVQYLFDDKKNSQKAENSEKPRFAIVLDGDGDRGFLLYYKKAEDTVYVLGGEH
                     LALILAPYLKKQRKKFWKNTCVYTIEADVLIANTFKNTHGLASHIVDVGDKWICNYPK
                     ANMLLGFESSGHIIVPTEIKNDEEKKEYRAGNGLYSCLLALYALWERNFDESFLTDYE
                     AAFSKTLYTYFIDKTLFYRNSSAWNQVQKSLEENLHSWIKTQETGFSFHEEQVSDTNV
                     LYYVIKKGKNLVGTLFIRNSGTENKNGVYLKCDQTLSTNLFAIAEKLAKLLSESIVNK
                     ECEEYKIQDEIFGILEKDAVFDDTSSYESLDAISETQLHTVLQALVKQNLIKREGNQY
                     KRSKSNDKIRFV"
     gene            complement(6257..8773)
                     /locus_tag="PKW26_00565"
     CDS             complement(6257..8773)
                     /locus_tag="PKW26_00565"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92090.1"
                     /translation="MHKKNGKCSFLPDGTIKLHESIQDKTRESWLMLLARRGQDNSTT
                     LCWGINNTGASFARIGTQEGDLINGFVHEDPYKTRLLGQYLYLVDEDKNYFTNSWYPV
                     LNKEQELETTFKFGSLQQKTSYTEIKVQTDSFIPNAFDGLIQLITIKNNSKKARKLKL
                     FAVNPVNIGDARGIQFAGFNSLMLGGAFHDEALNGTVWRSNFGISYDSEGFTAQENHD
                     ATKEMFGKLLLHTSSEKVQSYSTKYEDFVGHYSNTMANPEGLFYDSLRSVNAEELTSA
                     LSVLEFDLSLEAGEEKTITVSSVMCTTADYYVRNKAEYKAALAIANNPKKAKEELAAV
                     MADWEAEFAQNTLSIPGLDVVSHSFKWLQYQCAMVACLNRMKSRFHSGFEYGFGFRDI
                     LQDLLAILPYDNEKVKEILHFVAKQMFSDGNVYHNFYVSAPGTTDFNASDDPLWFILA
                     VCEYIKETHDFAILEEKAPFADLDVEKTGTIYEHLDIALRKVWRFSVDGLPLLFDADW
                     NDDLSSYPDHLSVMTAQILYKSLVEYKLLVEALPESSLSALKIQKDALLKDLVEKTET
                     VSKSINDYCIDEDGSYIRLLGPNKDASQSIGSSKTDGVTFFEPIAWSGYSGQATKEQF
                     DKAYEVCEKILKAKGGYRICQGDKRLVNKELPTDYTAWKRSAPGKKENGGMFRHLESW
                     YIASLCKFGYGDKAFDIFYKTLPFVCSEDDPYNYAAERFVYPEYVSGPDSNEYGRAGH
                     TWLTGTAPTRHKTIMESVFGIQPVHEGLKLDPCVPKAWKTFSAKRTIKKTVFNISYEN
                     PNAVEKGVVSLSVDGEVLPSNIIPLVFFDGKEHEVKVVMG"
     gene            8990..9988
                     /locus_tag="PKW26_00570"
     CDS             8990..9988
                     /locus_tag="PKW26_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ92091.1"
                     /translation="MSVTIHDVAKRAGVSHTTVSWTIHDHPGITEATKKKVFKAIEEL
                     DYHPNYLARSLVKGKTNTIAVIANFFSSPFEMEVLKGVEQGANNQKNPYMLTFYTTMG
                     KHEEVLKDILYGKRADAAILLSISPPKSISELFKKNKIPLIVIDEEVTKATAIQVDHF
                     KGSYLATEHLIHTGKKHLALVIGDKESEILSQRERARGFYKALADNKLPFEKDAIFTV
                     RDFYFEEGFSVFNSIHSAPFPIDGIFCAAGDVIAQGIMLEAKKNGCKIPEEYAIVGYD
                     DIPSSSLVYPALTTIRQPLFQIGKLAYEKAALMLVDKKIKEEVIVYSVDLVVREST"
     gene            complement(10015..10251)
                     /locus_tag="PKW26_00575"
     CDS             complement(10015..10251)
                     /locus_tag="PKW26_00575"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HOQ92092.1"
                     /translation="METDLTSDKNRLLEIEERLMKTETSLAHVEDFLVKTQEEALNLW
                     KEIEKVKRQNTRLENKIKELEELENLPHAKPPHY"
     gene            complement(10364..12112)
                     /locus_tag="PKW26_00580"
     CDS             complement(10364..12112)
                     /locus_tag="PKW26_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOQ92093.1"
                     /translation="MVSLTINDKEVQVEKGTTILDAAKKLHINIPTLCYTPDLPPWSS
                     CGICIVRVAGTSKMLRACCTEVANGMKLITHDAEILKARRTILELILSNHPSDCLQCE
                     RNTNCELQDIAAEFGLREKPRFTQILKNQAIDKSYEVIVFDRDKCINCGRCVEACQIM
                     QNVHAIEYTGRGDRTVIGPVAGANLSESPCVRCGQCAAHCPVGAITTQNPLTDVFEKL
                     SDPKLVSAVQIAPSVRVALGEEFGLPIGSISTGKIYTALRIIGFKYVFDTNFSADLTI
                     MEEGTELVDRLTNDGPLPMFTSCCPGWVDYCEKNYHDYLPHLSSAKSPQQMQGAITKT
                     YFANTIKAKAEDIYSVSIMPCTAKAYEARRDDSMYASGVSDVDKVLTTREFASLIRLA
                     GIDFAKLEESEADSPIGPYSGAGTIFGATGGVMEAAVRTAYKLVVGEELGDLDYTAVR
                     GLENVKVTEVNLKGKIIRLCVIHQLSSVEPVMAEIRKARDEGKEIPYHFVEVMACRGG
                     CVAGGGQPYGVTDEVRKLRAQALYQDDTASEIRTSHQNPLIQKIYTDFLEKPNSHKAH
                     ELLHTKYTKRDLYNIQ"
     gene            complement(12162..13943)
                     /locus_tag="PKW26_00585"
     CDS             complement(12162..13943)
                     /locus_tag="PKW26_00585"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOQ92094.1"
                     /translation="MAYTHYILVCGGTGCESSKADEIFNNLLTEAKNFGVEDKVQIVK
                     TGCFGFCEKGPIVKVLPEDSFYVEVKPEDAKTIIAEQIVKGREVKRLLYNQEEKNIEQ
                     VEEIQFYQKQYRIALRNCGIINPEDISEYIARDGYLALEKSLFEMQPKEIIDLLKKSN
                     LRGRGGAGFPTGIKWETAHKTKSDIKYVVCNADEGDPGAYMDRSIIEGDPHSVIEAMT
                     ICGIAIGANKGFVYIRAEYPLAIDRLKVAMNQAHEKGLLGDNILGSGFNFDIEIRLGA
                     GAFVCGEETALLRSIEGLRGMPTPKPPFPAISGLWGKPTIINNVETFANIPIIMTRGE
                     KWFNSIGTEDSKGTKVFALTGKIENSGLVEVPMGTTLREIVYEIGGGIKNGKKIKGVQ
                     TGGPSGGIITEEFLDTPIDFGNLTKLGSMMGSGGMIVMDEDDCVIDVAKFYMEFCLDE
                     SCGKCSPCRIGTRQLYTLLEKISDGRGELGDLQKLKDIGFAMQQGSLCALGQSAPNPI
                     LSTIAKFEQEYINHIVNKKCSSGKCKKLITYEITDKCIGCGLCARKCPVGCITGERKE
                     KHLIDQTQCIKCGECFNACKFDAILVN"
     gene            complement(13958..14353)
                     /locus_tag="PKW26_00590"
     CDS             complement(13958..14353)
                     /locus_tag="PKW26_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOQ92095.1"
                     /translation="MAKMTLEDLRKLRNETQTEIQRRETDGKSVQIIVGMGTCGIASG
                     AKLTLDSFITELKEKNLAHTTIIRQTGCMGFCANEPTVEVIAPDMPKVIYGNVSPDVS
                     KEIVEKHIIGKTLLDKLIIDYPSVDVVKK"
     gene            complement(14376..14918)
                     /locus_tag="PKW26_00595"
     CDS             complement(14376..14918)
                     /locus_tag="PKW26_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOQ92096.1"
                     /translation="MHYTICELIADLTQNAIEADAKVISVRVHETETELSFEIQDNGK
                     GMDSETLEKVRNPFYTDGIKHPNRKVGLGIPFLIQTANQTDGTWHIDSQKGKGTKLKA
                     VFSLSNIDTPPLGCLTSLFRQILSFPQNYEMHIDRIKETKDDSKNYSISRSELIEALG
                     ELETVESLDILKTFLESHEE"
     gene            complement(14931..15425)
                     /locus_tag="PKW26_00600"
     CDS             complement(14931..15425)
                     /locus_tag="PKW26_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOQ92097.1"
                     /translation="MNDVENAGFSKELNAFIKEWKNKPGNLIMILHKVQEEQGYISQD
                     AAKAISIKTGAHLAKVYGVMSFYHFFKTKKPGKFKISVCMGTACYLKGGQELIDETKK
                     LLGLEAEQDTTPDGLFSIEPVRCIGCCGLAPVLAINGDVYGKLTTKQIPDILDIYKNG
                     KEKN"
     gene            complement(15556..16299)
                     /locus_tag="PKW26_00605"
     CDS             complement(15556..16299)
                     /locus_tag="PKW26_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92098.1"
                     /translation="MQQKKIYLLFSLSLIFLLSSCAPGINIVVDETLAIDLEFASALE
                     ASIEPQIRSLMGLDAKSALFQKEAIETSLTKSGFSIDAILLPSPIAVEIESKKQILKN
                     NANPLAEFLSIEKHENKVCFTICISPEIMQNLIQSMPSEIQEYADLLMAPALTDEEME
                     AEEYLSLFSLVYGQSLSKAFENSFVKIAIRAPKKITSASVSHTDLASIHVDADTAHLS
                     LSLYALLALHKNSKPLANNKLIIELNWNS"
     gene            complement(16362..17426)
                     /locus_tag="PKW26_00610"
     CDS             complement(16362..17426)
                     /locus_tag="PKW26_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOQ92099.1"
                     /translation="MKRRIFIIALFFLILSHSFTQTNNRARKWIDEAKQYIGTPYLYG
                     GMSKNGIDCSGFIHITALNGIQLSLPRTTEALYDWVKIISDAQKEAGDLVFFKTVGSS
                     ISHVGLYMGNNQFIHAASSGPNTGVIVSSLSESYWAQRYAGVGRVLEENTLTQNTQTK
                     NTTTSAPKTKSSNQNKKNETQTAKTESSFGFLYNEYIQFEAGISADWSFYNIESIFLS
                     TRGFSAHAFLQTKKFPNNFGLGVDFNYDSFLSVFHIPIYFSLEINPNLKIYTGPVFSL
                     GSARIDEGNNSIDVKAPIYPLFMGISFESDIYNFFGMKGYLFQKINYTYYKTVTNEAL
                     SFYNGYRSSLVFKTGLQILL"
     gene            17553..19268
                     /locus_tag="PKW26_00615"
     CDS             17553..19268
                     /locus_tag="PKW26_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOQ92100.1"
                     /translation="MSKKTQQKPWDFLEEFRGSLFTADWPTLPQMFLISTKRYPDRAC
                     FTDFDAEKHTLSYTEVLEKVENLALWLKNNGVKKGDHIAVFGKNSPEWATVYLAAGFA
                     GAIIVPIDYGLHDKEIATLLAVSAPSFIFVDEEKYDVFKKSHGEKVFSLSKKKGDYVY
                     DLKGSGNFEMPELAEDDTAAILFTSGTTGNPKGVMLSHKNLVSDCYIAQTNMTIYHTD
                     VFYALLPIHHSYTMLAVFIEALSVGAEIVFGKSMAVTRMLKELKEGKITMLLGVPLLF
                     NKLVGGILKGIREKGVLVNAFMHFLLGFSYILKKYFKLNAGKVLFKSILEKVSLSTIR
                     IAICGGGPLAESVFKVYQALGIDFVQGYGLTETSPIVALNPTYNFKIESVGRNFHPFM
                     EMKILDPDEKGIGEVLIKGPMVMQGYYKMPEETKDVFTEDGWFKTGDLGRLDDEHYLY
                     LAGRAKNMIVTEGGKNVYPEEIENAFQLVTDIEQITVKGYIVDKATKSEGIEALIYPS
                     DDLFKRLNIVRGSDYSIDLVRDEIQEAVNKINKELVVYSRISKITILDEALEMTTTKK
                     VKRNF"
     gene            19281..19955
                     /gene="rsmG"
                     /locus_tag="PKW26_00620"
     CDS             19281..19955
                     /gene="rsmG"
                     /locus_tag="PKW26_00620"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOQ92101.1"
                     /translation="MNILENGLSCLGFDEKKRKAVLPQLEAYVSELLLFNKVYDLVGT
                     NSRDEIYTRHILDSLASFSTIQALVEERGFSEKIKPSFVDIGSGAGLPGIPLAICFPE
                     FSFSLLERMSRRCAFLENVLSVLQLGNVQVLNTEAEKYTGEGFDFAVFRAFRPLDIKM
                     TETILSLLKPEGLALAYKGKKDKIESEMKEIQALVGEDWKLKEIALPFLDSLERHLVI
                     FKAVSN"
     gene            complement(19936..21300)
                     /locus_tag="PKW26_00625"
     CDS             complement(19936..21300)
                     /locus_tag="PKW26_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ92102.1"
                     /translation="MDNLYTIVLVAFVLIVFTTFLLLATQVKKKSKRRLRPKSNQVLI
                     RDAIRKLDSDPHNPESLKILADIYFKESKWEKALPLFQTQLNIAAAYPQIDQFEAGLK
                     LGICSLKLGKPEDAVKPLLLARHERPENFEINYNLGEALIQTNDFEKAIPVLRKALTI
                     NTEANEVYASLATAFYKTKRFKEALPYLKKTLDINPESKEYLYYMADSLHETGNTDKA
                     LKIFMHLRADPLFGIYSCLTAGTIHFNQGHTDKAIRDFEIGLKHQSNDKDIQAEFRYK
                     LSQAYLKQNDIGKSLMYLKETNAIKPNYKDVPSLIARYEELNQNQNYRTYLIGGNSDF
                     VSLCRKLVLVYFKKSRAKITNINVFTDFAELTTDIETDKWEDTVFFRFYRTTSSVGEL
                     FIRDFHAKIRDEKAGRGICVTAGTFSDESKKFIEGRPIDLIEKDMLVKMLTSIESEPN
                     YLQL"
     gene            21415..22956
                     /locus_tag="PKW26_00630"
     CDS             21415..22956
                     /locus_tag="PKW26_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOQ92103.1"
                     /translation="MNNIVGIKLADGSFYPILEEGLASSKLLELTTVRDDQETVQLDL
                     YKAIDGDEKTLEYVDTLLIENLLPRPKEEPTLSLTLAIDDDDVLSASIEEAESGESTS
                     LSVSLLNSSEPTQLGSPDFALADSEQRMTLSSMPFMDDVPRERSNEEGLAETVDEFHT
                     EEDFAIDISSIEDESIDDFALDEATIDFETEQNQDTDDTILDEAEFEIDIPFEADAAD
                     VESDGLDDFDMNLDDETFDMGFDDSNEENLDIATDDELEIKLDEDSDEALASDEELTE
                     DNFPSFPDDLEISEPETSLDENEEEGFARSMYNFGDSLDDEIENQKINENDFNTEALD
                     DSIPAPEFSFSDLYEKDDYIEEEKVKEQKNEGTKRNWILLLLLVLLLLACLGLLVFLF
                     LGNRGDKTKIETHDGNTELVAENRFAAEPSVEPIPEEVKTDSAKENEIVKVDTEFVVP
                     EPVKETVPEAEAVQYKIKWGDTLWDISNAYYKTPWLYPKIASYNGIKNPDFIISGTWI
                     TIPPK"
     gene            22958..>25068
                     /locus_tag="PKW26_00635"
     CDS             22958..>25068
                     /locus_tag="PKW26_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOQ92104.1"
                     /translation="MIFLKAKNSFSFFKIGLVISIIFLNFSCYAQGGREEGFVKALVD
                     PKLSTKERLSAYTRAMQSKNPYIAEKATYESLVLNPSGTILKSAKKSFTKFHESGLVL
                     AKLVELDIEAGRLTDAQKLLEWSFQNTQNDAVLLSMQALQVRLLMAEKKDFQKELIVL
                     FTEGDIGKEHAYFKSEVFLESLPIDLELLLRLRFAVYERNYGFAYRTAEAFIENIQQS
                     ERKDLLYKRAILSDIGKAFLYGSTDYETNASFFETLARTLPRSLDMPSHSEVAFISYF
                     YAARILERSGSMYNEQVLSLYELSWREAIGADFDNALWYYLNLLEKISLPRLIQKIKE
                     TIFDWHDPSYFSDILERLSYRLLSSGEWQLFYELYRIIENQADSESQARFAYLSARLI
                     QIGLLSAEDVLAQSSFALEGESKEVASERLEELVLSLFSKAFYENHSSLYYRILAAEK
                     LDIPIEDTEGSFYSKKLTKKNTVNTALEEAILLYAKYGLYDLAYEIFLEHASEISFKT
                     ASAVAEYFARAGDVNGKLYPMSIRLAIRSVQSRDEALQKDILEKMYPRFFSEEVAEFA
                     AAFSLEEYLIYALIRSESLFEKKVVSSAGAIGLAQLMKPTAGDIARKLGLSDYDLGDA
                     ATNIRFGTFYLRELLDRLDGSALLALSSYNAGITRVRNWARNNQLPDDLFLETIPFAE
                     TREYSKKILSAAALYAYLYYD"
     assembly_gap    25069..25168
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25169..25606)
                     /locus_tag="PKW26_00640"
     CDS             complement(<25169..25606)
                     /locus_tag="PKW26_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOQ92105.1"
                     /translation="MKIKADLHIHSCLSPCGSLDMSPKRIVQELKQRDIALAALTDHN
                     TSLNCPAFASLCKAEGIQALFGMEVQSSEEIHILALFNELSASLDFSAFVYALIPNIK
                     NIPKKTGDQVYVDEDENILGELEKYLLNSIPLSIDEVAKEIHER"
     gene            complement(25641..25985)
                     /locus_tag="PKW26_00645"
     CDS             complement(25641..25985)
                     /locus_tag="PKW26_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92106.1"
                     /translation="MTLEEIAKRLKLKALYSDYENIFCTNAYCSDLLSDIMGNAKDES
                     ILISIQAHKNTVAVASLKDSPAIIICNNRPIGEDMIEACKDEGIALFVTGDNQFEVSA
                     KLYACLHKALPS"
     gene            complement(26021..27016)
                     /locus_tag="PKW26_00650"
     CDS             complement(26021..27016)
                     /locus_tag="PKW26_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOQ92107.1"
                     /translation="MPTIPLNTESSPEAVIELLFKLKVKDVMSTNIFTANPQTTMRDI
                     QNSMKKHYITGLPITEGDTLVGIISMDDIINAFDKNTIDEACESHMTRNLIVLQESMP
                     LSLAVSYFNKYKFGRFPVLNKKNKLTGIVTTTDVIAALLVAMNKEVERLEIEAQQAQD
                     ALQIEDEKQRKKRSKILEKAKNLEAFPNRLIEFKTEPFNFETAGKASTEIKKILRSMA
                     VDKALARRIGIASYELEINQVIHSKGGMMRYYISPENIVIEAIDTGPGIPDVDAALTE
                     GFSTASEQVRAFGFGAGMGLPNAKRVSDHFHISSKPGKNTTVRVQFNLSQEPETE"
     gene            complement(27041..27859)
                     /locus_tag="PKW26_00655"
     CDS             complement(27041..27859)
                     /locus_tag="PKW26_00655"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOQ92108.1"
                     /translation="MKNIKIIAIDLDDTLLREDLSISDYSVSILQKAAAQGYYITLAS
                     GRTDNAILPYVRRLDIAGREQGRYIISQNGAVILDLHTRLPIYERFVNPDVLIEVYRA
                     ARKKGLFPEVYSDSTIYVAEMNPWTERDMKLSGLKAEVIEDFENFLIKTHPKMIVPGE
                     PEVIKILQDELAHLHGKTCEIFTSKPYFLEILPHESGKGEALLYLAEKLGFSREETMC
                     FGDSWNDESMIRLAGESVAMKNAVDGIKKLAKHETEFTHNEDGVAHFIEKHLLF"
     gene            complement(27890..28702)
                     /locus_tag="PKW26_00660"
     CDS             complement(27890..28702)
                     /locus_tag="PKW26_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOQ92109.1"
                     /translation="MSIFQAIILGALQGITEFLPISSSGHLAIAQHLFGLELPLIFDV
                     SLHLASLLAVLLVFRKILWSLIISFGRWVMRKNSIEDELQLNTILSLILASIVTAAFG
                     LVFKKLIPDLPIIFIFGGFILTACALAASHKVHSREVEKNTKEPKKISVLKALVIGAS
                     QGIGVLPGISRSGITISSSLFLKLDRKTAGDFSFLLSIPAILGAFILEAKDLTGLTTE
                     VSLISLLIGCFTSFIVGYFSLRFLLKLIQKGKLYYFAFYLMPVAVLGFIFIH"
     gene            28784..29620
                     /gene="mazG"
                     /locus_tag="PKW26_00665"
     CDS             28784..29620
                     /gene="mazG"
                     /locus_tag="PKW26_00665"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOQ92110.1"
                     /translation="MEYKDSIEGASKSFGNFFSSIVRLRTPGGCPWDLEQTALSMRGD
                     LLEECFEAIEAINEENPEHVKEELGDLLLNLLVIAFIHEQEKQFTLSELIDAVNEKII
                     RRHPHVFEESEGKSYEKEGGAKTSDEVLAQWDSIKQGLEGRKKESILDEVSLGLPPLM
                     RAFKLQKKAAKKGFDWDNPEEVREKVEEELLELQEAKSADEREDEAGDLLFATVNYIR
                     HLGVDPSIALSRANAKFYKRFGHVERRMAETEGKSFTAKELDGFWKEAKDMDDKDRLE
                     YT"
     gene            29658..30275
                     /locus_tag="PKW26_00670"
     CDS             29658..30275
                     /locus_tag="PKW26_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOQ92111.1"
                     /translation="MKSILCEKKPFRVAEEEKAEEQKEAFSEALQAKFLKTRQILLTG
                     EVNKNLSEKIVRQLLILEADSDKPIYLFIDSPGGDVDAGYAIFDMIRFIKAPVYTVGM
                     GLVASAGALILLASPKERRVALPNSHYLIHQPLSGIKGVATDIEIHAQEIEKIRTKIN
                     ALIAEETGVKLSQVEKDTDRDFWMSAEEAKSYGLVGKIAATRDEL"
     gene            30279..31235
                     /locus_tag="PKW26_00675"
     CDS             30279..31235
                     /locus_tag="PKW26_00675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ92112.1"
                     /translation="MEFDLTDELIKQITFAMENQREIFLLDSCEKVLIKKTDIAQRLA
                     AEEKGENISFSETQNQNRFYTLPEWNSILGFKMMEKFVSVLKNPLARSDLKQVLSLGK
                     GVFRNFKNKLSEYPEVEKLWLNFKDAEMKKMVLDWYNRHRASWGLDLLPIEFNETDVL
                     ILEDFLFRFSSVAEEPEVFETYQNLFFDELCERAEEAEDEEVLAFLWKEYHFLQEERE
                     DVQLCIVETIEEDFAGIAAFFQLAGKRKKRFLIPFLYVEKNFRGLGLGKELLQRIRDY
                     ATEQGVRQLVVHNSFVPDVLVSVLLKNGFQKTKGIYFANVAD"
     gene            31358..31894
                     /locus_tag="PKW26_00680"
     CDS             31358..31894
                     /locus_tag="PKW26_00680"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HOQ92113.1"
                     /translation="MSFKHSKEKNVEKLDLFLEEAIHSIKTEEDPFELNNLKKVFKKS
                     VPFFMRSYVAVYMLKKLTNVSLREEKTSRSASSREKKAPYKRSREDFDESLYETLFFS
                     VGRKRRVYPKDIVGLLMQNAKLEKTQIGLIRVLDNYSFVQIMKEDAQKVIDLLNGFEF
                     RGRKLTVSYSRKKEEADS"
     gene            31918..32598
                     /locus_tag="PKW26_00685"
     CDS             31918..32598
                     /locus_tag="PKW26_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ92114.1"
                     /translation="MKDVFCLTGGVLGTNTYIVPLANNECFVVDPAVDAERIAEKIAS
                     AGLRLTAILLTHGHYDHMESLQELSLLYPQAKIAIHSEDAKSLGKDALLRHYAEFAST
                     GLVQLYAAFQGDFEKNPHLPEADFFLKEGEVFENEWLVLHTPGHTRGCICLYNEKKKQ
                     LLSGDTLFYMGWGRTDLEGGDQRALQTSLKRLFDLPSETKVFPGHEQYGFSLAETNAS
                     VTRGFLTI"
     gene            complement(32653..34308)
                     /locus_tag="PKW26_00690"
     CDS             complement(32653..34308)
                     /locus_tag="PKW26_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOQ92115.1"
                     /translation="MIVISILFKLVGSLSFLLFGMKLMSDGIQKSTGKSLHKALGFMT
                     KNRFMGLLTGLFVTMIIQSSGATTVMVVSFVNAGLITLAQSVGVIFGANIGTTVTAWI
                     VALFGFNFNIAALAIPLFGLGYLLRSVKKLKKETLGEAIMGFSLLFFGLDLLKNLLDV
                     NPEQISVFQSIFNHGLWSYILAIFLSIFFTALLHSSSAVSAIVITLSYQQLLSWEIAA
                     AMILGSNIGSTIDAIMASFGTKVNARRAALVHVMFNAFGFALAMFFFKPFLRLTDWIV
                     PGPVQNSITIHIAMLHTLFNIFSSLIFLPFTRQMAQLSELIIKPKKGEVPDIYTLTFI
                     KPSMKDNYEAYIIQAEKEIADMTDIAIDMFTTIQKGFINRTKDFVDEHFDKLSKQEDY
                     ADQMQEEISNFLIQYSHLPIADRTKHNVSIMLRIIDDLESITDECFSILLLLKRSIEK
                     EMTFNDEDMERLEPYGELVQQFLLFIKKNINKHLSRDQLRLAEVLEDQVDAFRKNLKK
                     IARKRLEKGADVRSELLYIDLVRYIEKIGDYAFSISEALAETK"
     gene            34435..35214
                     /locus_tag="PKW26_00695"
     CDS             34435..35214
                     /locus_tag="PKW26_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92116.1"
                     /translation="MTFSFIGCRNNSKKNAKNAIENTDADLQNLQKWQESDERIALVL
                     GLGYTEEHFVHELLSVLGDAFGLDCENGLIYPLLFPDDFVYNGVKGRISSLPDILMEK
                     KLRAVYIVGAPEGTHRALATMQDKKERALDIAVFSLFPQDDVLGIESGSDIVIDFGQF
                     YSPSPMSEEGGLEHLSLVPEILHSLIKKESLFDRNFSSKEKKTFLEKSLGLAWNVETS
                     VDLQTGLRSKNHFDISLIEEYSDAKAVVDSLIIIDKSTNKN"
     gene            35224..35907
                     /locus_tag="PKW26_00700"
     CDS             35224..35907
                     /locus_tag="PKW26_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOQ92117.1"
                     /translation="MPVLRQDESYLIGTAQAIKALGKKDKAYVLVVSDSHGDYDRFYA
                     LLEAYGSKLDALIFCGDGSFDLVGIIEAMANESPDSPLPDKILPPVIAFVRGNGDAES
                     YSYTYKGKNEVIIAPQAQNLTICGKKIFVAHGHKYSVDFGLDYAVEIAEKLGLDYFFY
                     GHTHIPREKKLKNTWFINPGSCSRPRGGFPPTCALLKLYKDEKEAACHFYEIKENRFG
                     NVDLKPMKI"
     gene            36029..37030
                     /locus_tag="PKW26_00705"
     CDS             36029..37030
                     /locus_tag="PKW26_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOQ92118.1"
                     /translation="MTEKKAPFLNGMVVLLASIIVFLFAIGLIVYASIDFSKNAIVLP
                     FVVSIVFGLLLSAIICPLLWAGLRILKPQEALVLTLFGKYYGTLFGAGFFFINPFVTA
                     MTVGVGNASSGSTGKSDTKTDLGLEVLKSFSMLSKPKKLSLKVMTLNNDKQKINDALG
                     NPIVVGVVVVWKIIDTAKAMFNVDNYVEYLSIQCDAALRNIVRLYPYDNTEDDGEKSL
                     RGSSLEVAEKLKEALREKVAVAGLEIVETNITHLSYAAEIAPAMLQRQQAVAVVAARQ
                     YIVDSAVGMVEMALDKLNKKNIVALDEERKAAMVSNLMVVLCAGKEAQPIVNAGTLY"
     gene            37050..37244
                     /locus_tag="PKW26_00710"
     CDS             37050..37244
                     /locus_tag="PKW26_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HOQ92119.1"
                     /translation="MAGKKTLEIDIKENTQEKKQVLLRISARLWNDLAGWAEDDFRSI
                     NGQIEFLLTEAVKKRKNKKD"
     gene            37373..38230
                     /locus_tag="PKW26_00715"
     CDS             37373..38230
                     /locus_tag="PKW26_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ92120.1"
                     /translation="MAIISLENVRKVYPLGKVEVEAVRGASLEIEKGSFVSIAGPSGS
                     GKSTILNMIGLIDTPSFGAVKIDGHDVYAGRFTSDIKKKKKDILPSKMDNKITDLRHE
                     YLGFIFQNFNLIPVLNVYENIEFPLLLGKQKMSKKERKEWIDYLIEKVGLTAWAHHKS
                     NELSGGQRQRVAIARALVTKAPVILADEPTANLDSKTGDQILNLMKEINRDLQTTFIF
                     STHDAKITAMAGHVIRLLDGDIVENTKIENGSTNNDAVQLACKKEKAQAEREKQVLPS
                     DTMSKDLGV"
     gene            38252..39769
                     /locus_tag="PKW26_00720"
     CDS             38252..39769
                     /locus_tag="PKW26_00720"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOQ92121.1"
                     /translation="MKNTIRIALGNLWEHKAKTLILGLLVCFGLMLIVLGTGFLEAAK
                     KGIEKDFRSHYTGDIVIHGPSPKKGVLTLFGVQENVTVGEMAKTPALPDAEALLSLLD
                     TVDGIQDRTRLISGMILLSSDAIGEGFIPRTEDESSFPMGFFLSGDENYFSMFPDVKI
                     IEGEMYNRNEPSLIMDLSLREKFETYYRIPLAVGDMITLQGYSAASAKLREVKIAGFF
                     AHPDPNTTMMSLCYLDQNTARALGDLTYGSVYARDLPQVIDTSLSTMDEDDLFADMFE
                     VENASILGSSLTDDDVDSLLGDTSLRDKLNEVADGAWQFVVLKLDRPEEASMKIIELN
                     ALFAEKEMNVKAIDWKTASSGFAMGTDILGAVFTILIVLLSVVVFIVIMNTLIISVIE
                     RTSEIGTMRALGAHRGFIRKLIFTETTCIVIIASLAGIVLALIISGIINISNIRITNE
                     TAKLFLGGGSFKITPNLGSTLAAFFAIFIGASLANLYPVSFALKITPLKAMNQED"
     gene            39778..41088
                     /locus_tag="PKW26_00725"
     CDS             39778..41088
                     /locus_tag="PKW26_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ92122.1"
                     /translation="MKTIGLAFRNLSRQKKRSFLLGGAIAFGFFIVTFVDALAGGSLR
                     NLSEQFSFLFGGNIFIQASQKDDDKVMTVTKDALFIQDVLKESGIRYTTEGKRTMLFG
                     TIIYEGQKAVTNISGCDFEKEKRLKDSISLVQGSWENMQDANALIIGETMAENLGIEV
                     GETVLFEMQTAKDYATVGEFKIAAINRDPSLLGSIMVYSHIAYLNELYELDEDAFFFY
                     TIMAEDHENQDALANQLETIIKKYKPVTSRLEAVKKNPLNIVRGINEQLKEASWEGDM
                     YSVITFDDQVPNIKQILSVVQYVSLGILVALFLVIMIGISNTYKMVIFERIKEIGTMR
                     ALGVKQKEAGRIFMWEAVLLSLFGSLVGFIVAIIGMNILSLFTFTSEELSLFLHNGHW
                     TYVLNAGSISIKLIVVVLLTMLAVRGSAKQAAKLNPAEALRTTR"
     gene            41097..41846
                     /locus_tag="PKW26_00730"
     CDS             41097..41846
                     /locus_tag="PKW26_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOQ92123.1"
                     /translation="MKKIIFIVLSIFLLFSVMAENTPDPYAILDVLQKQYSYDDQDYS
                     ATVTLIVEKPNQPAEQLQFKLFQRAAKKQFTMIQLLPETDKGAGYLQDGDNLWYYDPI
                     GRKFNHTSLKQNIGNSDAKAGDVVKEYSFRDDYEATSIESGKLGAFDVWIISLKAISS
                     RPAYAKEVYYIRKDITLLLKEENYSANDRLMRTILYPKYTRVLDRYLPIQIIMRDEVN
                     KGEQTQQILSEISTSAIPNKVFTKAYLEGLN"
     gene            41854..43248
                     /locus_tag="PKW26_00735"
     CDS             41854..43248
                     /locus_tag="PKW26_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92124.1"
                     /translation="MNKKYFYKQIFIFCLVFFLLGIKAFAIDSDDYLFSDDSFSDEGF
                     FSDAEFFSDEDLFSDDIFSSETLVETPVTKIDASLSSLIFETGSVRFGGSLSAGLHME
                     SIWIDPFSSDCDFLYGTEMGAIKLGGLENTGINPTLDLDLFFDSRPSSNTRLYGKFNV
                     NYPFNIFVDTALFPLPEAYVLTEIIVPNFSVFELFTDFNYDDKIYFRFGKHTVKWGVA
                     YFFSPADIINLAAIDPEDPEKQREGPISLRVHFPIEGTQTNLWFYILPDEKTMLVKDS
                     SFAFKAEFLVATSELGFGAWYRYNSPPHLMGTFTGSLAGKVALFAETVFAWGNEDAWQ
                     NNRVFTEMKPVVDATLGASYYWEAAKINLAGQYLFGFDFSNLDTKNYTGAHQAAFSLS
                     RSEFITKKMSLSAFTLMDILEHTGTASLNLGVDFFDGFSLSAGPALSRFATDSEKKIS
                     ASFSLSLRFGGGRF"
     gene            43317..44258
                     /locus_tag="PKW26_00740"
     CDS             43317..44258
                     /locus_tag="PKW26_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ92125.1"
                     /translation="MLEAKKLTKNYGDFCAVDALDFKADRGKIVGLIGPNGAGKSTTI
                     RMIMNILDVDSGEIVLDGQKFTASDSDKIGYLPEERGLYKKQKVIDVLYFLAELKSCS
                     KKDALPKIDYWLERFNLLEWKDKKIDALSKGMAQKIQFVSTIVHEPQILFLDEPFSGL
                     DPVSSDELLSVMNELKNAGRIILFSTHVMEQAERVCDQIIMLDRGKKILDGSVSQIKK
                     QQGSDSFSIRLDGDASFIEELSIVDRFSLKEKETCLVSLKTGTDANDLFSVIASESKK
                     QNKTIESITRNEPSLHDIFVKIAGKSIDESGDVKNEK"
     gene            44248..45483
                     /locus_tag="PKW26_00745"
     CDS             44248..45483
                     /locus_tag="PKW26_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ92126.1"
                     /translation="MKNKTLVVLKHEFNKIVKTKTFIIMTILGPFLLAAIMIVPMYFS
                     MKSVEGKAESLKIGIYLADSSQEVLAEVIVEESTALGWELFISKDRESLYTQAIKKEI
                     AGYLTIEEDAIKYFTLKPGDFTVNASLQSIISDYLIDERLSARGLSYEEVKALTQAYS
                     LPVFKISEDNETGVESDEGMEFALAIIIPMIFSLMIYMSVLLYGQMIGRSVVTEKSSK
                     VVDVLLSSVKPNQLMMGKIFGVGLAGMLQYSIWIFMALVALFVTKLVFGFAIPAQIAM
                     SNFVYLGIYFVLGYLLYGSLFAAVGSASEDEQHLGQLSLPLILFLVVPIMLISAIIEN
                     PNSTLSLVFGFFPLTAPLVMLSRITTGEIPLWQIPLSIGSLALCIWFFIRLSAKIFKT
                     GILMSGKNFKLREIVKWLR"
     gene            complement(45480..46217)
                     /locus_tag="PKW26_00750"
     CDS             complement(45480..46217)
                     /locus_tag="PKW26_00750"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOQ92127.1"
                     /translation="MRQYYIPLLSENLSENADAIMASYEVEGIELFYPWPLHKPRSLF
                     TLAFIAAGIDGIAVSFCAEAPKNKLLSKKTSHKMRVFEDDCLEFFIRPEESDIYYGFE
                     FNSKGFLLDYRCGIGEKGKQLILDSIDEKNKKIVQDTDAIVSGCSEAKNGDEILFFDY
                     SWKSKALISNTIEDEFWYAEIFIPWSDFGLRDFDYTSLDKKIWSFTCNRIDNPGNGIQ
                     GYQTLLASSEKESFHQPELFAKALFES"
     gene            46369..47535
                     /gene="nagA"
                     /locus_tag="PKW26_00755"
     CDS             46369..47535
                     /gene="nagA"
                     /locus_tag="PKW26_00755"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOQ92128.1"
                     /translation="MSICLTNGTLISGYYALEKMSILIEDGKVVDVFNQKRFEQKKFP
                     PTVRIIDVEGSFISPGLIDTHIHGFNGHGTDNCSPEAMLAMSEDLAQYGVTAFNPTLY
                     PSSEESMTKTIQSIVKAMGKENGAKIMGIHLEGPFISADKLGVQRPETVKTVDLELME
                     RLWQASEGHIVNMTVAPEIKGMRDLALYCIKKGIVAQAGHTNAEYAHMVEGMQAGILH
                     ATHLFNAMSQMHHRNPGAVGAVLIHPEMSCEIIPDGIHVHPDLLKFVARDKPIDKIVL
                     VTDALTPTEQSGSPLYANGEEVTFSGGCFHRVKDDVIAGSGLTMIKGVKNLVAYGFSV
                     EKAIRCGTANPAEIMKYGTKGVVSPGSDADIIVFDKHFNVLLSMVEGKIVKNLI"
     gene            47577..>47940
                     /locus_tag="PKW26_00760"
     CDS             47577..>47940
                     /locus_tag="PKW26_00760"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002665046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOQ92129.1"
                     /translation="MRVIVQPDYEACSLWAANHIARRINEAAPTAKKPFVLGLPTGGT
                     PIGTYRALIALNKAGKVSFEHVVTFNMDEYVGLPSDHPQSYHRFMWDNFFNHVNIKKE
                     NVHILNGMAQDLDAECEAY"
BASE COUNT        14127 a         8550 c         9914 g        15249 t
ORIGIN      
        1 tggcttaaag gaatttgaag ggcaacaagg ggcgggagac cagggcatga tgttcggctt
       61 tgcttgtaag gaaactccag agctcatgcc ggcgcctctt atgtatgcgc atagtatttt
      121 aagaaaggct tctgagcttc gaaaaacaaa gacaattccc tggcttcgcc ctgactcaaa
      181 aagccaagta acaattgcct atgaaggttt tacaccggtg agaattgact ctgtggttgt
      241 ttcacatcaa catgacccag atgtacccta tgaaaccata aaagaaacaa taatcgaaag
      301 cattattaaa ccgacgcttg agtcaagcgc cttactcgat aaggaaacaa agttttatat
      361 aaatcctacg gggcgttttg tcatcggagg accttttgga gacaccggtc taaccgggcg
      421 aaaactcatt gtcgacagct atggtggaat gggtcgccat ggaggcggcg cattttctgg
      481 taaggacccg tcaaaggttg accgttctgg agcctatatg gcacgctata tcgccaagca
      541 tgttgttgct gcagaccttg ctgagcgctg tgaggtacaa ctcgcatacg cgattggggt
      601 gccatatcct gtatctatct ttgttgaaac ctttggcaca ggaaaggttg aggagcttgc
      661 tatttcaaaa gcgatagaag aagtttttga ctgtagtcct gcaggtataa ttaaaaagct
      721 cgacttaaaa aagcccatat ataaaaaaac agcttcctat gggcattttg gacgagatga
      781 tttttcctgg gaaaaacttg acttagttga tgctttgaaa aaagctataa aatagcgagc
      841 cagtcgattc tggctttgtt ctagctgtga agggtttgct ttgaaagaaa agaaagaatc
      901 aaaaaaactc cttgaaaaag acgcttgttc tattgtctta caggatgaga gaactatctt
      961 tgagattttt tcgctctttc aaaaagcaaa tcctgaaccg aagaccgaac ttcttgcgcc
     1021 aaatgctttt actcttcttg tttctgttgt tttatctgcc caggctactg atcggagcgt
     1081 aaataaggcg acggaagcct tgtacaaaaa agtgcaaaca ccagaagcga tgcttgcctt
     1141 aggtgaagag ggcttaaaag agcatattaa aacaataggc ttgtatcgag taaaggccgg
     1201 ctatgtgatt cgcctgtcga aaattttggt agaagatttt cagtcgaaga ttcccttaaa
     1261 tcgagaagac ttagaaagcc ttccgggagt aggaagaaaa acagcatcgg ttatattgaa
     1321 tgttttggct ggagaagcga ccatgcctgt tgatacgcat ttgcttcgta tctcgccgcg
     1381 aattggcctt tccgatggta agactccctt gcaagttgaa aaggacttac tcgagagaat
     1441 cccaaaagaa tttttacagc atgcacatca ttggcttatc ctgcatggac gctatgtttg
     1501 tattgctcga aatccaaaat gtgaaagctg tctcatatcg catctttgta aaaaaaattg
     1561 cctgtagaaa tctactatac tttaataaaa gcgtaaaaaa acgctccttc ccttgaaaac
     1621 aaaggaagga gctaagttcc ataggagaca ggataaggag gaatcctatt ttagttagcg
     1681 cccatgctaa taataacgta gtgctctgca ttatcaccga agcttggtaa aataacagag
     1741 tctttttcac tttgtatttc ttttccatcg agagtcacac gagcaattcc cgtttcaact
     1801 ccctctggat tttcaaagtc aatactatat accgcatttt gatattttct ttttatagaa
     1861 aatgccttcc attctgaaga gacgcagggt tttattcgca agccttcaag aactggttta
     1921 accccaaaaa tagattcgat aattgttttg tgcatagttg gcgcagttcc tgtaagccag
     1981 gtatgaccgc ctcggccttc ttcttgtgca cctttcccac ggacgtactc ggggtagacg
     2041 tagggctcac aggagtaaga gtcttggtct ttcgaaagat tcatcggtaa ggttttttca
     2101 tacatagcga gcgccttatt gccttgccct gttttacagt aggcttctac aagccatgcg
     2161 tttaagtgca taaaaatact accgttttct tttaatcctg gtggttcttt ttctatattc
     2221 caggttcttt gcggtaattc atcacgatac gcgagtccgt taaaatacgg atagcaaagc
     2281 attgcacccc aaggggagtc gagttctttt tctacatgag cgagtagctc ttttcctttt
     2341 tctaggtcca ctgtttcatt tgtaatagca aatatttgtg gctctaaaaa gatttttgct
     2401 tcaaggtttt cgcttgagcc aaggtcttcg cttgcatctt ttacagaaag cgcacgttta
     2461 aaatagcctt ttttatcaac agcgtattta agaagtgctt cccgtgtttc gttttctgcc
     2521 tttgcatagt ctgagagttt tttctcaatt tcagcatttt gtggcagttt gtttttcata
     2581 agcttttgga attcatgcaa ggcgccaacg agctgttgcg caaccattat agattccatg
     2641 tttttggaag gctcatcgtt tacgcgtttt tcgtttaagt catcattcca atcgcaatct
     2701 ttaagatagg gaagatttct ctcgcttcta tctgtaataa ctcggtctaa gcttacaaaa
     2761 caatgctcga ggattgtgcc ctttgcttcg gtattgtctt cacgtttttc tgcgtagtaa
     2821 cttacttcat tgaggaagtc gtaatctccc gtttcatagc agtattgaat aactgcctgc
     2881 acaaaccaca gcgggtcgtc gcttgcttca atggatttat ttccaggctg gcttataaaa
     2941 aagttgtggt agcttatgcc tgtcgtaaaa atttgtgtag atatatgcaa taaaagttct
     3001 ctcataagct cagggcgata gggtaaaaga tataaaatat cttgtaaaat atcgcggaaa
     3061 ccccaaccgt attcgtagcc tgtatgaaag cggcttttca ttcggtttaa tataagaaca
     3121 atttcgcatt gatattggag ccattgaaaa gaagtattta catctttgtt tgggcttttt
     3181 atttgcaagc tttcaagacg tttcctccac cactcttcga tatctgccaa gagcgcttct
     3241 cgtttttctt tggtagaaat agcttctaga tgcgctttaa gttccgtttt attgtttaga
     3301 taataatccg acgttttatc gcttatcaag ctcaatgcaa agtcgattgt ttcaccaggt
     3361 tttaagctta caatatgttg gaaaacaccg caggggcgac gaagatgagg aatatctctg
     3421 tttgaaagtt ttgattctaa aatcgtcttt ggatttgtgt gattatgaaa tctgtctcca
     3481 tagaaatgca ggaggtcgct ttcataggct tggttttctt tgtttaagct tgagtgaaat
     3541 aaaacagttg ttccaagcat gccgttaatt ttttctggat catcatttac ttcgattcca
     3601 tgatgccttc taaaaacgag tccttttaaa tctgcatcgt attttacacc accaaaaaag
     3661 gcgtcgttat ctccagccat gagtctttga agcgcatcac ctatgtgcat gggaacaaag
     3721 ggatagagtt cgagttcttt ttcttcgttt gttgtgtttg taattgagac attttgtacc
     3781 cagccgtcaa agatttttgg tacaaacatg gaaacctttg tatcgatgcc cttatagctt
     3841 gattcaaaga ggatttttcc aaaagaaaag gtagtggtaa atttttgttc ttcttttctc
     3901 gtgggttgcc aacttgtaga ccatatttca tttgtttttt tgtctttgat atagatacag
     3961 cgaacgaggg agtcttcttc ttgaaggtga atctttgtat cgcttaaggt gttttcatgg
     4021 gtgccttgaa tatcaaagcc gccaccagcg tgggttacgc ctgaacaaaa cgttgaggta
     4081 gatttttgct ctggtcggat aagatattgc aaccaagctt caggtgtgta ggggttgtga
     4141 ataacaaatt cgtttttttc attaaacgaa ccgtattttg tcattgcttt tgctcctttt
     4201 gtattgattt ccttctcttt ttatgaggtt ttgtttaaca agagcctgta aaacggtatg
     4261 caattgtgtt tcgctgattg catctaggct ttcgtatgag ctagtatcat cgaaaacagc
     4321 atctttttca agaatgccaa aaatctcgtc ttgtatttta tactcttcgc attctttgtt
     4381 tacaatactt tcagagagta attttgcaag tttttctgca atcgcaaata agtttgtact
     4441 taaagtctgg tcgcatttta gatagacccc atttttattt tccgtgcctg aattacgtat
     4501 aaaaagcgtt ccaaccaggt ttttaccttt ttttataaca taatagagga cattcgtgtc
     4561 ggaaacttgt tcttcgtgga aggaaaagcc agtttcttgt gtttttatcc aactatggag
     4621 attttcttca aggctttttt gcacttgatt ccatgccgaa gaatttctgt agaacaatgt
     4681 tttatcaata aaataggtat aaagggtttt tgaaaaagca gcttcatagt cagttaaaaa
     4741 actctcgtca aagtttcttt cccaaagagc gtagagggcg agaaggcaag aatagagtcc
     4801 gtttccagcc ctgtattctt ttttttcttc atcgtttttt atttccgttg gaacaataat
     4861 atgcccagag gattcaaagc caaggagcat atttgctttt ggataattgc aaatccattt
     4921 gtccccaaca tcgacaatat gcgaggcaag cccatgcgta tttttaaagg tatttgctat
     4981 aagtacatct gcttcaattg tatacacaca ggtatttttc caaaactttt ttctttgttt
     5041 tttcaaatac ggtgcaagta taagggctag atgctcgcca ccgaggacgt aaacagtatc
     5101 ttcagctttt ttataataaa gtaaaaatcc tctgtcccca tcgccatcta ggacgatggc
     5161 aaaacgcggt ttttctgagt tttcagcttt ttggctgttt tttttatcgt caaaaagata
     5221 ttgtacgatt gcaggagcag atgtatagtc tttttttgtg tacaggctat gcccttcaat
     5281 ttctgccaca ccgcattgtt tattgatatt tgtgcctatt ggtttttcgt ttgggcttgc
     5341 aaaagctatc ttttgttttg caaaataata ctgcgcaatt tctgcccagg cgccatttgc
     5401 attatcgagg tagagatcga tggcattgag cgcttcccct atgtttagcg gaagcctctc
     5461 atctaagaat cttgtaaaat caatttcttt ttttgttgca tgggaggctt gtcgaatttc
     5521 tatacttgta tttatagaag agcgttccaa aaacatatag gcggatagct cataatcccc
     5581 gtttttccct tctggcaaaa gctttttaga tttaataaaa aacttaatgc cattttggtc
     5641 agcgggattg tggcttgcgg taagcatagc tcctgcatca atgccatcta tgagcatctg
     5701 gtagggtaca gctggggttg gcacaatacc gaggtcaaaa accttgtgtc caaagttttc
     5761 aagtccatgg ttcattgcat ttgtaagcgc ccagtttgtc gcaatatcgc gtccatcatt
     5821 tcctgtgcaa acattgcttc gttttgtaat tgtcccattg tcggaaagca tttttataaa
     5881 agcttttgag ctcagataga gcaactcagg tgaaagtatg agattttgag taaaatcgag
     5941 tataggattc tgtgatttct ccgtagcaat cttgcctctc attccatctg ttcccataaa
     6001 gctaaaacga gattgcaaaa aaagctcttt gagtattttt ttactagggt tttcctgact
     6061 atctactaaa gaaacaaact tttcaaagag aatgagcact tttaaaggat aggaccttac
     6121 ataggaactc tttaaaaatt cttttacctg aagagcaagc tgcggttttt ttacaaagct
     6181 ttcaatttca tatccaaacg gatcgatttc ttcaaaatta ttatttataa aaatacattg
     6241 tgccatattt ttctacttac cccatgacta cctttacctc atgttctttt ccatcaaaga
     6301 aaacaagagg tataatattg cttggtaaaa cttctccatc gacagaaaga gaaacgacac
     6361 ctttttctac agcgtttgga ttttcatagc tgatattaaa aacagttttt ttaatagttc
     6421 tttttgcaga aaaggttttc caagctttcg gtacacaagg atctagtttt aagccctcat
     6481 gtactggttg aatgccaaaa acagattcca taattgtttt atgtcgcgtt ggtgctgttc
     6541 cggtaagcca agtatgtcct gctcgcccgt attcattgct atctggtccg cttacgtatt
     6601 caggatacac aaatcgttct gctgcatagt tatatgggtc atcttcacta cacacaaagg
     6661 gcaatgtttt gtagaaaata tcaaaggctt tgtctccata gccaaattta caaagagatg
     6721 ctatatacca gctttcaaga tgtcggaaca taccgccgtt ttcttttttc cccggtgcac
     6781 ttctcttcca tgcagtgtaa tctgttggca attctttgtt tacaaggcgt ttatctccct
     6841 gacaaatacg gtagccaccc tttgctttaa gaattttttc acaaacctca taggccttgt
     6901 caaactgctc ttttgttgct tgtccagaat agcctgacca tgcgatgggc tcaaagaagg
     6961 ttacgccatc ggtttttgaa ctccctatgg actgacttgc atctttgttt ggtccgagaa
     7021 gacgaatata actgccatct tcgtcgatgc agtagtcatt tatgctcttt gaaacagttt
     7081 cagttttttc aactaagtct tttaaaagtg catctttttg aattttaagt gcgctaagag
     7141 agctttctgg aagcgcttct acgaggagtt tgtactctac aagtgattta tataagattt
     7201 gtgcagtcat aacagaaaga tggtctgggt agctacttaa gtcgtcgttc cagtcagcat
     7261 caaaaagtag aggtaagcca tctacagaaa atcgccatac ttttcgtagc gcaatgtcga
     7321 gatgttcata gatagttccc gttttttcta catctaagtc tgcaaaaggt gctttttctt
     7381 caaggattgc aaagtcatgt gtctctttta tatactcaca tactgcaaga ataaaccaaa
     7441 gtgggtcatc actcgcgtta aaatcggttg taccaggagc tgaaacataa aagttgtggt
     7501 agacatttcc gtcgctaaac atttgctttg caacaaagtg taaaatttct tttactttct
     7561 cattatcgta ggggaggata gcaagtaagt cttgtaaaat atcacgaaag ccaaagccgt
     7621 attcaaagcc tgagtgaaaa cgacttttca ttcgatttag acaagcaacc attgcacatt
     7681 gatattgcag ccatttaaaa gagtgagaaa ccacatcaag tccaggaata gagagcgtat
     7741 tttgtgcaaa ttcagcttcc cagtctgcca taacagctgc gagttcttct tttgcctttt
     7801 ttggattgtt agcgatagca agagccgcct tgtattcagc cttatttcgt acataataat
     7861 cagcggttgt gcacataaca gagctaacag tgattgtttt ttcctctcct gcttcgagag
     7921 aaaggtcaaa ttctagaaca gataaggcgc ttgtaagctc ttcagcattt acgcttcgaa
     7981 gggaatcata gaaaagaccc tcagggtttg ccatagtatt gctgtagtgc ccaacaaaat
     8041 cttcgtattt tgtagagtag ctttgtactt tttcactaga agtatgtaaa agaagttttc
     8101 caaacatttc ttttgtcgcg tcgtgatttt cttgcgcggt aaagccttcg ctatcgtagg
     8161 aaatgccaaa gttgctcctc cacactgttc cgttgagtgc ctcgtcatgg aaggctccgc
     8221 caagcataag gctattgaag ccagcaaatt gtatgcctct tgcatctccg atattaacag
     8281 ggtttactgc aaagagtttg agcttgcgag ctttttttga gttattttta atggttatga
     8341 gttgaataag accatcaaat gcgtttggaa taaaggagtc tgtttgaact ttaatctctg
     8401 tatagcttgt tttttgttga agactgccaa atttaaaggt tgtttcgagt tcttgttctt
     8461 tgtttaaaac aggataccaa gagtttgtaa aatagttttt atcttcatcg acaaggtaga
     8521 gatattgtcc taaaaggcgt gttttgtacg ggtcttcgtg gacaaagcca tttataaggt
     8581 cgccttcttg cgtaccaata cgtgcaaaag atgctccggt attatttata ccccaacaga
     8641 gggtagtgct gttatcctgt ccgcggcgag ctaagagcat gagccatgat tcacgtgttt
     8701 tgtcttgtat tgattcatgt agcttaattg ttccatctgg taaaaaagag catttaccat
     8761 tttttttgtg catttatagt ctccaaaaat tagtatttca agtggttttc acgtgaaaat
     8821 tatattctat tttaatacat atgtcaagtt ttattgggaa aactacgttt tttctttatt
     8881 atctgcatta tttattttgt ttttacgtaa aagcttgtat tttttctggt attcgagtat
     8941 tattagctta atatgaaaat gtaattttta tttaataagg atgtaaaata tgtcggtaac
     9001 tattcatgat gttgctaaaa gagcaggggt atctcataca acagtttctt ggacgattca
     9061 cgatcatcct ggcataaccg aagcgacaaa aaagaaagtt tttaaagcta ttgaagaact
     9121 tgattatcat ccgaactatt tggcacggag ccttgttaaa ggaaaaacaa atacaattgc
     9181 agtaattgct aattttttct cctcgccttt cgaaatggag gttttaaagg gcgttgaaca
     9241 aggtgcaaat aatcaaaaaa atccttatat gcttaccttt tatacaacta tgggaaagca
     9301 tgaagaggtt ctaaaagata ttctttatgg gaaaagagca gatgccgcta ttttactgag
     9361 catatcaccc ccaaaatcga tatctgagct ttttaaaaaa aataaaatcc ccttaatagt
     9421 tattgacgaa gaagtgacta aagcgacagc tatacaggtt gatcacttca aaggttccta
     9481 ccttgctact gaacatttaa tacatacagg aaaaaaacat ctcgctttag ttataggtga
     9541 taaagaatct gaaattttaa gtcagaggga aagagcgaga ggcttctata aagcacttgc
     9601 cgataacaag ctaccgtttg aaaaagacgc aatatttacc gtaagggatt tttattttga
     9661 agaaggtttt agtgtcttta acagtattca tagtgcacct ttcccaatcg atggaatttt
     9721 ttgtgcagca ggtgatgtta ttgcgcaagg aattatgctt gaggcaaaaa agaatggctg
     9781 taaaatacca gaagaatatg cgattgttgg ttacgatgac ataccttctt cgtccttggt
     9841 ctatcccgct cttactacaa ttcggcagcc gctttttcaa atagggaagc ttgcttatga
     9901 aaaggctgct ctcatgctag tagataaaaa aataaaagaa gaagttatcg tatattctgt
     9961 tgatttagtt gtaagagaat ctacctaaac tacattgttt ttcctttttc tcgctcaata
    10021 atgcggtggt tttgcatgag gtagattttc aagttcttct agctctttta ttttattttc
    10081 tagacgggta ttttgtcgtt ttactttttc tatttccttc cataagttga gtgcttcctc
    10141 ttgtgttttc actaaaaaat cttctacatg cgcaaggctt gtttcagttt tcatgaggcg
    10201 ctcttcaatt tctagaagtc tgtttttatc gcttgttaag tctgtttcca taattctatg
    10261 atagggtcta gctaaaaaga atacaagagc tatataaaaa aactagctca aaatagagct
    10321 agttttttat ttttatatac aaatactatc gaatattagc aacttattgt atgttgtata
    10381 aatcgcgttt tgtatatttt gtgtgtaaaa gctcgtgtgc tttatggctg tttggctttt
    10441 ctaaaaaatc tgtgtagatt ttttgtatca gtgggttttg atgacttgtt cgaatttcgc
    10501 ttgctgtatc atcttggtag agagcttgcg cgcgaagttt tcgaacctca tctgttacgc
    10561 catagggttg tccaccacct gcaacgcagc caccacgaca agccataact tctacaaagt
    10621 ggtagggaat ttcctttcct tcatctcgtg cttttcgtat ttctgccata actggttcta
    10681 cgcttgaaag ttggtggata acgcaaagtc ggattatttt tcccttgaga ttaacttcgg
    10741 taacttttac attttccaag ccgcgcactg ctgtgtaatc aaggtcgcca agttcctctc
    10801 caacaacgag cttatatgct gttcgaacag ctgcttccat aactccacca gttgcaccaa
    10861 aaattgttcc ggctccagaa tatggtccga tagggctatc tgcttcggat tcttcgagtt
    10921 tcgcaaaatc gatgccggcg agacggatta aagatgcaaa ttctcttgtt gttaagactt
    10981 tatctacatc ggaaacaccg gaagcgtaca ttgaatcatc tcggcgcgct tcgtatgctt
    11041 ttgcggtaca gggcatgatt gagacggaat aaatgtcttc tgcttttgct ttaattgtgt
    11101 ttgcaaagta ggtttttgta attgctcctt gcatctgctg tggacttttc gcacttgata
    11161 agtgtggtag ataatcatga taattttttt cacaataatc gacccaacca ggacagcagc
    11221 ttgtaaacat tggtagaggt ccgtcgtttg taagtctgtc tacgagctct gttccttctt
    11281 ccattatggt aaggtcagca gaaaagtttg tatcaaatac atacttaaag cctataatgc
    11341 gtagagcggt atagattttc cctgtgctaa tagaaccgat gggaagtcca aattcttcgc
    11401 cgagggcgac tcgaactgaa ggagctattt gtactgcaga aacaagtttt gggtccgaaa
    11461 gtttttcaaa tacatctgtg agtggatttt gtgttgtgat tgcacctaca gggcagtggg
    11521 cagcacattg cccacagcga acgcagggac tttcagaaag atttgcacct gctacaggac
    11581 caatcactgt tctgtctcca cgaccagtgt attctattgc gtgcacattt tgcattattt
    11641 gacaggcttc aacacagcgt ccgcagttga tacatttatc tcggtcaaaa acaataactt
    11701 cataggactt atctatggct tggtttttga gtatttgtgt aaatcgaggt ttttcgcgaa
    11761 gaccaaactc ggcggcaata tcttgtagtt cgcaattggt attgcgttcg cattgcaaac
    11821 aatcgctggg atgatttgat aaaatcaact ccaaaatggt acggcgggct ttgagaattt
    11881 ctgcgtcatg tgtgatgagt ttcatgccat tggctacttc tgtgcaacat gcgcgaagca
    11941 tctttgaagt cccagctaca cgcacaatac agattccaca tgaagaccaa ggtggtagat
    12001 ctggagtata gcagagtgtt ggtatgttta tatgtaattt ttttgcagcg tcgaggattg
    12061 ttgtgccttt ttcgacctgc acttctttgt catttatagt taggcttacc atgtatttct
    12121 cctattttac ctttttgtca aaggtatatt ctaataaaat tttagttcac aagaattgca
    12181 tcaaacttac aggcgttaaa gcattcacca catttaatac attgagtttg atcaataagg
    12241 tgtttttctt ttcgctctcc ggtaatacat ccaacagggc attttcgagc acaaagacca
    12301 catccaatac acttgtcagt gatttcatag gtaataagtt ttttacattt accagaagag
    12361 cattttttat ttacgatgtg gtttatatat tcttgctcga attttgcgat agttgaaaga
    12421 atagggtttg gtgcagattg tccaagtgca cagagtgagc cttgttgcat agcaaaacct
    12481 atgtctttta gcttttgtaa atcgcccaat tcacctctgc cgtctgagat tttttcaaga
    12541 agcgtgtaga gctgtcgtgt ccctatgcga caaggcgaac attttccaca agactcatcg
    12601 aggcaaaatt ccatatagaa ttttgcaacg tcgataacac aatcatcttc gtccataaca
    12661 atcataccgc cagaacccat catggagcct agttttgtaa gatttccaaa gtcgattggt
    12721 gtatctaaaa attcttctgt gataataccg ccagaaggcc caccggtttg tacacctttt
    12781 attttcttgc cgttttttat gccaccacca atttcatata caatttcgcg caaggtagtt
    12841 cccatgggga cttctacaag acctgagttt tcgatttttc ctgtaagtgc aaaaaccttt
    12901 gttccctttg agtcttctgt cccaatagaa ttgaaccatt tttcaccacg agtcataata
    12961 ataggaatgt ttgcaaaggt ttcgacgtta ttgataatgg ttggttttcc ccaaagtcca
    13021 gaaatagctg ggaagggtgg ttttggcgtg ggcattccgc gtaagccttc gatagagcgt
    13081 agcagagcgg tttcttcgcc acaaacaaag gcacctgcac ccaagcgaat ttcaatgtca
    13141 aaattaaaac ctgatccgag gatattatct ccaagcaagc ctttttcgtg ggcttgattc
    13201 atagcgactt tcaagcgatc tatagcaagc ggatattcag cacgaatata gacaaaaccc
    13261 ttgtttgcac cgatagctat accacaaatc gtcatcgctt cgattacaga gtgcggatct
    13321 ccttcgatga tagaacggtc catgtaggca cctggatcac cttcgtcggc attacaaaca
    13381 acgtatttta tatcgctttt tgttttatgg gctgtttccc attttatgcc agtagggaag
    13441 cccgcaccac cgcgaccgcg aagatttgat tttttgagaa ggtcaataat ttcttttggt
    13501 tgcatctcaa aaagtgattt ttctagggca aggtagccat cccgcgctat gtattcgctt
    13561 atatcttctg ggttgataat accacagttt cgaagtgcta ttcggtattg cttttgatag
    13621 aattgaattt cttcaacttg ttcaatgttt ttttcttctt gattatagag gagccgtttt
    13681 acttcacgcc cctttacgat ttgttctgca ataatagttt ttgcatcttc tggttttact
    13741 tctacataaa acgaatcttc gggaagtact tttacaattg gacctttttc acaaaagcca
    13801 aaacaacccg tttttacgat ttgtacctta tcttctacac caaagttttt tgcttcggta
    13861 agtagattgt taaaaatttc gtctgccttt gaggattcac atcctgttcc tccgcaaact
    13921 aaaatataat gtgtataagc catgtattca ccttacctta tttttttacg acatcaactg
    13981 aaggatagtc aataatgagt ttatccaaaa gtgttttacc aataatatgt ttttcaacaa
    14041 tttcttttga aacatcggga cttacattgc catagataac ctttggcata tctggtgcta
    14101 tcacctcaac ggttggttcg ttcgcacaaa aacccatgca accagtttgt ctaataattg
    14161 ttgtatgtgc aagatttttt tctttcaatt ctgtaatgaa tgaatccaag gtcaattttg
    14221 caccgcttgc aataccgcat gttcccatac caacaattat ctgtacgctt ttgccatctg
    14281 tttcacgtct ttgaatttct gtctgtgttt catttcgaag ttttctcaaa tcttctaatg
    14341 tcatctttgc cataatttct cctgtttgct attttctatt cttcgtgact ttctaaaaaa
    14401 gtcttgagta tgtcgagtga ttcaactgtt tccaattctc ctaaggcttc gatgagctca
    14461 ctgcgagaaa tagagtagtt ttttgaatcg tctttagttt ccttgatacg atcgatgtgc
    14521 atttcatagt tttgagggaa actaagtatt tgtctaaaaa ggcttgtgag gcaaccaagg
    14581 ggcggtgtgt caatatttga taaggaaaaa acagctttaa gctttgttcc tttccctttt
    14641 tgtgaatcaa tatgccatgt gccatcggtt tggtttgcag tttgtataag aaatggtatg
    14701 ccaagtccta cttttctgtt tgggtgtttt atcccgtcgg tatagaaagg atttcttact
    14761 ttttccaaag tttcactgtc catgcctttt ccattgtctt gtatttcaaa agaaagttcg
    14821 gtttctgttt catgcactcg aacagatata acttttgcat ctgcttcaat tgcgttttgc
    14881 gttaggtcag ctatcaattc acagattgtg tagtgcattt aaatcgaacc ttagtttttt
    14941 tcttttccat tcttgtaaat gtcaagaata tcggggattt gttttgttgt gagttttccg
    15001 tagacatcac cgttaatagc caaaacagga gccagcccac aacaaccgat acagcgaaca
    15061 ggttctatgg agaagagccc atctggagtt gtatcttgtt ctgcctcaag ccctaaaagt
    15121 ttttttgttt catctatgag ctcttgcccg cctttgagat aacaggcagt tcccatacaa
    15181 acggagattt taaatttccc tggttttttt gtcttaaaaa aatggtagaa ggacataaca
    15241 ccatagactt ttgctaaatg tgcgccagtt ttaattgaaa ttgccttggc agcatcttgg
    15301 gaaatatagc cttgctcttc ttgcaccttg tggagaatca ttataagatt tcccggctta
    15361 tttttccatt ctttgataaa tgcattaagc tcttttgaaa agcctgcatt ctctacatca
    15421 ttcatacagc ccccattaaa taatatatcg ataaaatatg cactattata acgaaaatat
    15481 aaacttgtgg caagagtagg gattttttct ttggctttat atcgataaaa ttttatctat
    15541 ataaagctcg tgtttttagc tattccagtt cagttcaatt ataagtttgt tgttggctag
    15601 gggcttggag tttttgtgaa gtgcaagcaa ggcatagagg ctcaaagaaa gatgcgcagt
    15661 atctgcatcg acatgaatcg aggctagatc tgtatgggaa acagatgcgc ttgtaatttt
    15721 ttttggagct ctaatagcaa tcttgacaaa ggaattttca aaagcctttg aaaggctttg
    15781 tccatagacg agggaaaaga gagaaaggta ttcttctgct tccatttctt catcggtaag
    15841 ggcaggcgcc ataagtaagt cggcatactc ttgaatttcg cttggcatac tttgtatgag
    15901 attttgcata atttcggggc ttatacagat agtaaagcat accttgtttt catgcttttc
    15961 aatgctgagg aattctgcaa gtggatttgc attgtttttg agtatttgct ttttgctttc
    16021 aatttctact gcaatcggtg atggaagtag aattgcatcg atactaaaac ctgatttagt
    16081 taagctggtt tcaattgcct ctttttgaaa taaggcgctt ttagcatcaa gtcccatgag
    16141 gctacggatc tgcggttcaa tgctcgcttc caatgcgctt gcaaactcta agtctatagc
    16201 aagtgtttca tctacaacaa tgtttatgcc tggtgcacaa gatgagagta aaaaaataag
    16261 gctaagagaa aagagaagat aaatcttttt ttgttgcata gctatcctta aaaatggttt
    16321 ttcaattatc tgaaagttga aagtattagt attctttcat tttataggag tatttgcaag
    16381 cctgttttga aaacaagact cgacctatat ccattataaa aagacagggc ttcattggtt
    16441 acagttttgt aataggtata gtttattttt tgaaaaagat agcccttcat accaaaaaaa
    16501 ttgtagatat cgctttcaaa agagataccc ataaaaaggg gataaatagg cgccttgaca
    16561 tcgatactat tgtttccctc atctatgcgt gcggagccca gagaaaagac aggtcctgtg
    16621 taaatcttaa ggtttgggtt tatttctagg ctaaaataga taggtatgtg aaaaacagat
    16681 aaaaaactat catagttgaa gtcgactccg agaccaaaat tgttgggaaa tttttttgtc
    16741 tgaaggaagg cgtgagcgct aaagcctctt gtgctcaaaa aaatagattc tatattatag
    16801 aaactccaat ccgcacttat acccgcttca aattgtatat actcgttata taaaaagcca
    16861 aaagagcttt cagtttttgc tgtttgtgtt tcgttctttt tgttttgatt agaagacttt
    16921 gtttttggag cacttgtcgt tgtgtttttc gtttgcgtgt tttgtgtcaa agtattttct
    16981 tcaagtacac gaccaactcc tgcatagcgt tgtgcccagt agctttctga aagcgatgag
    17041 actattacgc cagtgtttgg accgctactc gctgcgtgga taaattgatt atttcccata
    17101 tataaaccga catgagaaat acttgagccg accgttttga aaaaaacgag atctccagct
    17161 tctttttgtg catcggagat tatttttacc cagtcataga gcgcttctgt tgttcgggga
    17221 aggctgagtt gaatgccatt gagggcggtt atatggataa aacctgaaca gtcaatgccg
    17281 ttttttgaca taccaccgta gaggtaggga gtcccgatat actgctttgc ctcatcgatc
    17341 catttacgag cgcgattatt tgtctgcgta aaactatgcg agagaatgag aaaaaataag
    17401 gctataataa aaatacgtcg tttcatactc atagtatcgg aagcttagca tgtttttata
    17461 tataaatgct aaaaaatctt cttttttgca accttttttc ttattttgtg tcatactaat
    17521 gagggttttt taatatttaa agaggaataa aaatgagtaa aaaaacacaa caaaagcctt
    17581 gggacttttt agaggaattt agaggaagcc tctttacagc tgactggcct accttgcctc
    17641 aaatgttttt aatatcgacg aagcgctatc ctgatagagc ttgttttact gactttgatg
    17701 cggaaaaaca cacgctttct tatacagagg ttttggaaaa ggtagaaaac cttgctcttt
    17761 ggttaaaaaa taatggcgtg aaaaaaggtg atcatatagc agtttttggg aagaactctc
    17821 ccgaatgggc aacggtttat ttagcagcag gttttgcagg agcaatcatt gttcccatag
    17881 actacggtct ccacgataaa gagattgcta ccttattggc tgtttctgca ccctctttta
    17941 tttttgttga cgaagaaaaa tacgatgttt ttaaaaaatc tcatggtgaa aaagtctttt
    18001 ctcttagtaa aaagaaaggc gactatgtct atgacttaaa aggttctgga aactttgaaa
    18061 tgcctgaact tgcagaagat gataccgcag ctattttatt tacctctggt actacaggaa
    18121 atccaaaagg tgttatgctt tcgcataaaa atcttgtctc agattgctat atcgcacaaa
    18181 caaatatgac tatttatcat accgatgttt tttatgcctt acttcctatt catcactcat
    18241 atacgatgct tgcggtattt attgaagctc tttcagttgg tgccgaaatt gtgtttggaa
    18301 agagcatggc tgtaacgaga atgctcaaag agctcaaaga aggcaaaatt acgatgcttt
    18361 taggagtacc tctcttgttt aataagctcg taggaggcat tctcaaaggc attcgagaaa
    18421 agggtgtttt ggtaaatgca tttatgcact tccttctcgg attttcctat atcttaaaaa
    18481 aatattttaa gctcaatgca ggcaaggttt tgtttaagtc cattcttgaa aaggttagct
    18541 tatctaccat acgaattgct atttgtggtg gaggaccgct tgcggaaagt gtttttaagg
    18601 tttatcaagc cttgggtatc gattttgtac aaggttatgg tttaacagaa acatcgccca
    18661 tcgttgcgct caatccaacc tataatttta aaatcgaaag tgttggacga aattttcatc
    18721 cctttatgga aatgaaaatt ctcgatccag atgaaaaagg cataggagaa gtgcttataa
    18781 aaggtcccat ggttatgcag ggctactata aaatgcctga agaaacaaag gacgttttta
    18841 ccgaagacgg ttggtttaag acgggcgact tggggcgact tgatgatgaa cactatctct
    18901 accttgccgg tcgtgcaaaa aatatgattg taaccgaagg tggaaaaaat gtgtatccag
    18961 aggagataga aaatgccttc caattggtaa cagacatcga acaaataact gtcaaaggct
    19021 atattgtcga taaggcaaca aaatccgaag ggattgaggc gcttatttat ccttctgacg
    19081 atttgtttaa gcgactaaat attgttcgtg gaagtgatta ttctatagac cttgtacgag
    19141 atgagattca agaagcggta aataaaatca ataaggaact cgttgtctac tcgcgtatca
    19201 gtaaaataac gatactcgac gaagccttgg aaatgacaac taccaaaaaa gtaaaacgaa
    19261 acttttagat tgggaaataa atgaatatct tggaaaatgg gcttagctgt cttggctttg
    19321 atgaaaaaaa acgaaaggca gttttgcccc agctagaagc ctatgtctca gagctcctgc
    19381 tttttaacaa agtctatgat ttggtcggaa caaactcgcg cgatgagata tatactcgcc
    19441 atattttaga ttctcttgcc tcctttagca cgatacaagc tttggtagaa gagcgtggct
    19501 tttcagaaaa gataaaacca tcttttgtag atattggttc tggggcaggg cttcctggca
    19561 taccgctcgc tatttgtttt ccagagtttt cttttagcct gctcgaaaga atgtctcgcc
    19621 gttgtgcctt tttggaaaat gttctttctg ttttacagct agggaatgta caggtactca
    19681 ataccgaagc agaaaaatat accggagaag gctttgattt tgcggttttt cgcgcctttc
    19741 gcccccttga tataaaaatg acagaaacta ttttgtcttt gcttaaacca gaaggactcg
    19801 ctcttgctta taagggaaaa aaagacaaaa tagaaagcga aatgaaagaa atccaggcgc
    19861 ttgttgggga ggattggaag ctaaaggaaa ttgctcttcc ctttttagat tctctcgaaa
    19921 gacatcttgt gatttttaaa gctgtaagta attaggctca gattcaattg aggtgagcat
    19981 ttttacgagc atatcctttt ctattaaatc aatagggcgt ccctcaataa attttttgct
    20041 ttcatctgaa aaagtccctg cagttacaca aataccgcga cccgcttttt catctcggat
    20101 tttggcatga aaatctcgta taaaaagctc tccaacagaa ctcgttgttc gataaaaccg
    20161 aaagaaaaca gtatcttccc atttatcagt ttcaatatct gtggttaatt cagcaaaatc
    20221 agtaaataca ttgatatttg taatttttgc cctcgatttt ttgaaataca caagaacgag
    20281 ttttctacac agcgatacaa agtcgctgtt accacctatg agataggttc ggtagttttg
    20341 gttttggttc aattcttcgt agcgtgcaat cagggaggga acatctttat aatttggctt
    20401 tattgcattg gtttctttta aatacattaa acttttgccg atatcgtttt gctttaagta
    20461 ggcttgtgaa agcttatagc gaaattctgc ttgaatatcc ttgtcatttg actgatgttt
    20521 taagccgatt tcaaaatcac gaattgcctt gtctgtgtgc ccttggttaa agtgaattgt
    20581 tcctgcggtg aggcaagaat atatgccaaa gaggggatct gcgcgtaaat gcataaatat
    20641 cttcaaagct ttgtcggtat tgcccgtttc atggagtgaa tctgccatat aatagagata
    20701 ttctttgctt tctgggttta tatcgagggt tttctttaag tagggaagtg cttctttgaa
    20761 gcgttttgtt ttataaaagg ctgttgcaag gcttgcatag acttcattcg cttctgtgtt
    20821 aatagttaaa gcctttcgta aaacaggtat tgctttttca aaatcatttg tttgtataag
    20881 tgcttcgcct aggttataat tgatttcaaa gttctcgggc ctttcatgtc gcgctaagag
    20941 caggggtttt acagcatctt caggtttacc gagtttaagg gagcagattc cgagttttaa
    21001 gcctgcttca aactggtcaa tttgtggata tgctgctgca atattcagtt gtgtttggaa
    21061 caagggcaga gctttttccc atttgctttc tttaaaatat atgtcagcta aaatttttaa
    21121 gctttctgga ttatgtgggt ctgaatcaag cttacggata gcgtctctta taagcacttg
    21181 gttgcttttg gggcgtaatc ttcgctttga ttttttcttt acctgtgtgg ctaagagtaa
    21241 aaaagtagta aatacaatga gcacaaaggc aactaaaaca attgtatata aattatccat
    21301 atcggttcta ttatgccttg catctctagt gatgtcaata atttaaatag ctaaagattt
    21361 tttaaattat tgacaaaata gggtctgcat actaccatgg agacaggtga ttgtatgaat
    21421 aatattgttg gaataaaatt ggcggatggg agcttctatc cgattttgga agaaggtctt
    21481 gcttcatcaa agcttttgga acttacgact gttcgagatg atcaggaaac ggttcagctc
    21541 gatttgtata aagcgattga tggggatgag aagactctcg agtatgttga tacgctgctt
    21601 atagaaaatt tactgcctcg ccccaaggaa gagccaacgc tctccctcac gcttgcaata
    21661 gatgacgatg atgttttgtc agcttctatc gaagaggcgg aatctggtga gtcaacttct
    21721 ctgagcgtat ccctattgaa tagttcagag cccacgcagc tgggaagccc tgactttgcc
    21781 ctcgcagata gcgaacaaag aatgacgctt tcatccatgc cctttatgga cgatgtccct
    21841 cgagaaaggt caaatgaaga aggccttgct gaaactgttg atgaatttca tacagaagaa
    21901 gattttgcta ttgatataag ctctattgag gatgaaagta tagatgattt tgcccttgat
    21961 gaagcgacga tagactttga aacagaacaa aatcaagata ctgatgatac gatacttgat
    22021 gaagccgaat ttgagataga tatccccttt gaagctgatg cggctgacgt agaatctgac
    22081 gggcttgatg actttgatat gaatctagat gatgagacct ttgacatggg ctttgatgat
    22141 agcaatgaag aaaatcttga tatagccact gatgatgagc ttgaaataaa acttgatgaa
    22201 gacagcgatg aagctcttgc ttctgatgaa gagcttacag aagataattt tcccagcttc
    22261 cctgatgatt tagaaatttc agaaccagag acgagtcttg atgaaaatga agaagaaggc
    22321 tttgctcgtt caatgtataa ttttggggat agcctcgatg atgaaatcga aaatcaaaaa
    22381 ataaatgaaa atgattttaa taccgaagct cttgacgatt ctatacctgc gccagagttt
    22441 agcttttctg atctctacga aaaagatgat tacatagagg aagagaaagt aaaagagcag
    22501 aaaaatgaag gtacaaaaag aaactggatt ttgcttttac ttcttgtatt acttctatta
    22561 gcttgtcttg gcttgctggt ctttcttttt ttgggaaata gaggagataa aaccaagata
    22621 gaaactcatg acgggaatac agagcttgtt gccgaaaatc gctttgctgc tgagccttct
    22681 gtcgaaccaa ttcctgaaga ggtaaaaaca gactcggcaa aagagaatga aattgtaaag
    22741 gttgatacgg agtttgttgt tccagaaccg gtaaaagaaa ctgtgccaga agctgaagca
    22801 gttcagtata aaatcaaatg gggagatacc ctctgggata tttccaatgc ctattataaa
    22861 acaccttggt tgtatccgaa aattgcaagt tataatggga taaaaaatcc agattttata
    22921 atttcgggaa catggattac aataccgcca aaataatatg atttttctaa aagctaaaaa
    22981 ctcattctca ttttttaaaa tcgggcttgt aataagcatc atttttctga atttttcttg
    23041 ctatgcacag ggaggaagag aagaaggctt tgtaaaagcg cttgtagatc caaagctttc
    23101 aacaaaggag cgactttctg catatacgcg ggcgatgcag agtaaaaacc cctatattgc
    23161 ggaaaaagcg acctacgaaa gtcttgtctt gaatccttcg ggaactattt taaaatctgc
    23221 aaaaaaatct tttacaaagt ttcatgaaag tggcctcgtt ttagcgaagc ttgtagagct
    23281 agatatcgag gcaggaaggc taaccgatgc gcaaaaactt ttggagtgga gctttcagaa
    23341 tacgcaaaac gacgcagttc tgctgagtat gcaggcactt caagtgcgac tcttgatggc
    23401 agaaaagaag gattttcaaa aagagcttat tgttttattt acagaaggcg atataggaaa
    23461 agaacatgcc tattttaagt cagaagtttt tcttgaatcg cttcctatag atttagaact
    23521 tttacttcgc ttacgctttg ctgtctatga aagaaactat ggctttgcct acagaacagc
    23581 tgaagctttt atcgagaaca tacagcaaag tgaaagaaaa gacctcttgt ataaacgtgc
    23641 tattctttcg gatataggga aggctttttt atacggaagt acagactatg agacaaatgc
    23701 gtcttttttt gaaaccctag cgcgaactct ccctcgctcc ctagatatgc cctcacattc
    23761 tgaagtggct tttatatctt acttttatgc agcgcgtatt ttggaaagaa gtggaagcat
    23821 gtataatgaa caagtacttt ccctctacga attgtcatgg cgagaggcaa taggtgcaga
    23881 ttttgataat gcgctttggt attatctcaa tcttttggaa aaaatatctc ttccgcgtct
    23941 tatccaaaaa ataaaagaga cgatttttga ttggcatgat ccatcgtatt tttcagatat
    24001 tttggagcgc ctttcctatc gtttactctc aagcggtgaa tggcagcttt tttacgagct
    24061 gtatcgaatt atagaaaacc aagccgatag cgaaagtcaa gcgcgctttg cctatctttc
    24121 tgcgcgactt atacaaatag gtttgttgtc tgcagaagat gtcttagcgc aatcgagttt
    24181 tgcattagaa ggcgagtcta aagaagttgc gtctgaaagg ctggaagaac ttgttctatc
    24241 cttgttttca aaagcatttt atgaaaatca tagctccttg tattatcgta tattagctgc
    24301 tgaaaaactc gatataccca tagaagatac tgagggaagt ttttatagca agaagctcac
    24361 aaaaaaaaat acggtgaata ctgctttgga agaagcgatc cttttgtatg caaaatatgg
    24421 actctacgat cttgcctatg agattttttt ggagcatgca agtgaaatat cttttaaaac
    24481 agcttctgct gtagcagagt attttgcaag ggcaggcgac gtaaatggca agctctatcc
    24541 tatgtcaatt cgtcttgcta ttcgctcggt acagagtcga gacgaggcat tgcaaaaaga
    24601 cattctagag aagatgtatc cgcgattttt ttcagaagag gttgcggagt ttgcagctgc
    24661 tttttctctc gaagaatatc tcatctatgc tttgattcga agtgaaagtt tgtttgaaaa
    24721 aaaagttgtg tcttcggcgg gggctatagg acttgcccaa ctgatgaagc caacggcagg
    24781 ggatattgca cgaaagcttg gcttgagcga ttatgactta ggcgatgccg ctacgaatat
    24841 tcgctttggg actttttact tgcgagagct cttggacagg ctcgacggtt ctgctttgtt
    24901 ggccctatcg tcatacaatg caggtataac acgtgtgcgg aattgggctc gaaataacca
    24961 attgcctgat gacttatttt tggaaacaat accctttgca gaaacgcgcg aatatagcaa
    25021 aaaaatcctc tccgccgcag ctctctatgc gtacttgtat tatgatttnn nnnnnnnnnn
    25081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25141 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc gttcgtgtat ttctttggca acttcgtcga
    25201 tagaaagcgg aatagagttt agaagatatt tttcgagctc tcctaaaatg ttttcatctt
    25261 catccacata gacttggtcg cctgtttttt tgggaatgtt ttttatattt ggaataagag
    25321 catacacaaa ggctgaaaaa tcgagggagg cggagagctc attaaaaagt gcgagtatat
    25381 gaatttcttc agagctttgt acttccatgc caaaaagagc ttgtattcct tctgctttgc
    25441 aaagactcgc aaaggctggg cagtttaagc ttgtgttgtg gtcagtgagg gctgccaggg
    25501 ctatgtccct ttgttttagt tcttgaacaa tgcgctttgg tgacatatcg agacttccac
    25561 aaggagaaag acaggaatga atatggaggt cagctttgat tttcataatg cgcttccttg
    25621 aaccttttta agaatttttt ttaacttgga agagccttgt gtaagcatgc gtagagcttg
    25681 gcggacactt caaattgatt atcgcccgta acaaagaggg ctatcccttc atctttgcaa
    25741 gcctctatca tatcttcacc gatggggcga ttgttacaaa taattatcgc aggagaatcc
    25801 ttgaggcttg cgacagcgac ggtgtttttg tgtgcttgaa tgcttataag gatagattca
    25861 tcttttgcat ttcccattat atcgcttaaa agatcagagc aataggcgtt tgtacaaaaa
    25921 atattttcgt agtcgctata caaggctttt agttttaatc tttttgcaat ttcttccaat
    25981 gtcatatcgt atctcctcta aaaaaagtcg ggttctttgt ctattctgtt tctggttctt
    26041 gagataaatt aaactgaacg cgcaccgtgg tgttttttcc cggctttgag cttatgtgga
    26101 agtggtcgga aacacgtttt gcattgggta agcccatacc tgcaccaaag ccaaaggctc
    26161 tcacttgttc gcttgcggta gaaaagcctt ctgtgagggc ggcatcgaca tcgggaatcc
    26221 ccgggcctgt atctatcgct tcgattacga tattttcggg gctaatataa tagcgcatca
    26281 taccaccctt tgagtgtatg acttgattta tctccaattc atagcttgca ataccgattc
    26341 ttcttgcaag tgccttgtca acagccatgc tccgaagaat tttttttatt tcggttgaag
    26401 ctttccctgc cgtttcaaag ttaaagggct ctgttttaaa ttctatgagc cgatttggaa
    26461 aagcttcaag gttttttgcc ttttccagta tttttgagcg tttttttctt tgcttttcat
    26521 cttctatttg caaggcatct tgcgcttgct gcgcttcaat ttcaaggcgc tcaacttcct
    26581 tgttcattgc aacgagaagg gcagcgataa catcagtggt ggtaacaatg cccgtgagtt
    26641 tgtttttttt attcaataca ggaaagcgcc caaacttata tttattaaaa taagataccg
    26701 caagggaaag tggcatactt tcttgcaata caatgagatt tcgagtcata tgtgattcgc
    26761 atgcttcatc gattgtgttt ttgtcaaagg cattgataat gtcgtccata gaaataatgc
    26821 caacgagggt atcgccttca gttatgggta ggccagttat atagtgcttt ttcatactgt
    26881 tttgaatatc gcgcatagtc gtttgtgggt ttgcggtaaa gatattcgtc gacattacgt
    26941 ccttgacctt gagtttaaaa agtaattcta ttactgcttc tggggaagat tcagtattta
    27001 atggaatcgt cggcatgaac ttaagtatag ccgatttttt ttaaaataac aaatgttttt
    27061 ctataaaatg tgcaacgcca tcttcgttat gtgtgaactc ggtttcatgt tttgcaagct
    27121 ttttaatgcc atctactgca tttttcatag cgacactctc tcccgctaag cgtatcatag
    27181 attcatcgtt ccagctgtcg ccaaaacaca tggtttcttc tcgtgaaaag cccaactttt
    27241 ctgctagata caagagagct tcgcccttgc cgctttcgtg gggtagaatt tcaagaaagt
    27301 agggtttact tgtaaatatt tcacatgttt ttccatgtaa atgtgctaac tcatcttgca
    27361 agatttttat cacttctggt tccccgggaa ctatcatctt tggatgcgtt ttgataagaa
    27421 agttttcaaa atcttctata acttctgcct ttaaaccgct gagtttcatg tcgcgctctg
    27481 tccagggatt catctctgca acgtaaatag tgctgtcgct atacacttcg ggaaacaagc
    27541 ctttttttcg tgctgcccta tacacttcga tgagtacatc agggttgacg aagcgctcat
    27601 atataggcag tcgcgtatgc aagtcgagga tgaccgcacc attttgagaa ataatgtagc
    27661 gtccttgttc tcttcctgct atgtcgagac ggcgaacata ggggagaata gcgttatctg
    27721 ttcgaccaga agcaagcgta atataatagc cctgtgctgc agctttttgc aaaatagaaa
    27781 cagaataatc agaaatacta aggtcctctc tcaagagtgt atcgtctaaa tcgatggcaa
    27841 taatttttat atttttcatg tttcctatcc tttcgtattg tatgtttttt tagtgtataa
    27901 aaataaaacc caaaacagcg acgggcatta aataaaaggc aaagtaatag agttttcctt
    27961 tttgaatgag cttgagtaaa aagcgtaatg aaaaatagcc gacgataaag gaggtaaaac
    28021 agccgattaa aagacttata agtgatactt ctgttgtaag gcctgttaaa tcttttgctt
    28081 cgagtataaa ggcccccaag atagcaggga tagaaagcaa gaaggaaaag tcacctgccg
    28141 tttttctgtc aagttttaag aaaagcgagc ttgaaatagt gataccactt cgtgaaatac
    28201 ctggtaaaac gccgatacct tgtgaggcgc ctatgacaag cgctttgagg acggagattt
    28261 tttttggctc ttttgtattt ttttcaactt ctctgctatg taccttatga cttgccgcaa
    28321 gggcgcaggc ggtgagaata aagccaccga aaataaatat aatcggcaag tctgggatga
    28381 gttttttgaa tacaagtcca aaagctgcag ttacaatgct tgcgagaatg aggcttagaa
    28441 tagtgttgag ttgtagctca tcttctatgc tgttttttcg cataacccaa cgaccaaagc
    28501 ttataataag cgaccagagt atctttctaa aaacgaggag taccgcgagg aggcttgcaa
    28561 ggtggaggga aacgtcgaag atgaggggaa gttcgaggcc aaaaagatgt tgagctatgg
    28621 cgagatgccc tgagctcgaa ataggtaaaa attctgtgat gccttgtaag gctcccaaga
    28681 taattgcttg aaatatagac ataaatctac tctagcatgc aaggcaatgg gtttcaagac
    28741 cttcttttat tgtgtatgat agcaggatta ggaagttata attatggaat ataaagatag
    28801 tatagaaggg gcatcaaaga gctttggcaa ttttttttct tctattgtgc ggctaagaac
    28861 accaggtggt tgcccctggg atttagaaca aacagctctt tcgatgcgtg gagatttact
    28921 cgaagaatgt tttgaggcaa ttgaggcaat aaacgaagaa aatccagaac atgtaaaaga
    28981 agaactcggt gatttgcttt taaatctttt ggtgattgca tttattcatg aacaagaaaa
    29041 gcagtttaca ctttctgagc tcatagatgc ggtaaatgaa aagattatac gtcggcatcc
    29101 ccatgttttt gaagagtctg aaggaaaaag ctatgaaaaa gagggaggag caaaaacaag
    29161 tgacgaggtg cttgcacagt gggattcaat aaaacaaggt ctagaaggca gaaaaaaaga
    29221 atcgattttg gatgaggttt ctttgggctt accacctttg atgcgggctt ttaagttgca
    29281 aaaaaaagca gctaaaaaag gctttgactg ggataatcca gaagaagtgc gtgaaaaggt
    29341 agaggaagaa cttttggaac tccaagaagc aaaaagtgca gacgaaagag aagatgaggc
    29401 aggagatctg ctttttgcaa cggtgaatta tattcgtcat ttgggagtgg atccgagtat
    29461 tgctttatca cgggcaaatg caaagtttta taaacgtttt gggcatgttg aacggcgcat
    29521 ggcagaaact gagggaaaat cttttactgc aaaagaattg gatggatttt ggaaggaagc
    29581 aaaagatatg gacgataaag acagattaga gtatacttaa aagagctatg attttatgca
    29641 atatatggag ttagaaaatg aaaagcatac tatgtgaaaa aaaacctttt agagtcgctg
    29701 aagaagaaaa ggctgaagag caaaaagagg ccttttcaga agctttacag gcaaagtttc
    29761 tgaaaacaag gcaaatatta ttgacagggg aggtgaataa aaatctttcg gaaaaaatag
    29821 tccgtcaatt acttattttg gaagcagact ccgacaagcc aatatatctt tttatcgatt
    29881 cgccaggagg cgacgtcgat gctggctatg caatctttga tatgattcgt tttattaagg
    29941 ctccggtgta tacagttggc atgggactcg tagcgagtgc tggtgcgctt atcttacttg
    30001 cgtcgcctaa agaaaggcgt gtcgctctac caaatagtca ttaccttatt caccaaccgc
    30061 tgagtgggat taagggtgtt gcaactgata tagagattca tgcgcaagaa attgaaaaaa
    30121 tacgcacgaa gataaatgca ctcattgccg aggaaacagg agtcaagctg tctcaggttg
    30181 aaaaagatac agacagagat ttttggatgt cagcggaaga agcaaaatcc tatggcttag
    30241 tcggaaaaat agcagcgacg agggatgagt tgtaaaaaat ggaatttgat ttaacggatg
    30301 agttgataaa acaaataaca ttcgctatgg aaaatcaaag agaaattttt ttattggata
    30361 gctgtgaaaa ggttttgata aaaaaaactg atattgcgca aaggcttgca gcagaagaaa
    30421 aaggagagaa tatatctttt tctgaaacac aaaaccaaaa tagattttat accttgcctg
    30481 agtggaattc tatcttgggt tttaaaatga tggaaaagtt tgtatctgtt ttgaaaaacc
    30541 ctctcgctcg ttcagatcta aagcaggttt tatccttggg taagggagtt tttagaaact
    30601 tcaaaaacaa actttccgag tatcctgaag ttgaaaaact ttggcttaat tttaaagatg
    30661 ctgaaatgaa aaaaatggtg ctcgattggt ataatcggca tcgagcttct tggggtttag
    30721 acttgctacc gatagaattt aacgaaacag atgtgcttat cttggaagat tttttgtttc
    30781 gatttagttc agtagcggaa gagcctgagg tttttgaaac ctatcaaaat cttttttttg
    30841 atgaactttg tgagcgtgcc gaagaagcgg aagatgaaga ggttttggct tttttgtgga
    30901 aggagtatca ttttttacaa gaagagagag aggatgttca gctctgtata gttgaaacta
    30961 tcgaagagga ttttgcaggc attgctgctt tttttcaatt agcagggaaa aggaaaaaaa
    31021 gatttttaat tcccttcctc tatgttgaaa aaaactttag aggtttagga ctgggaaaag
    31081 aacttttgca gcgtattcga gattatgcta ccgagcaagg cgtgcgacaa cttgtcgttc
    31141 acaactcctt tgtgccggat gttttggttt cagttttact gaaaaacggt tttcaaaaaa
    31201 caaagggtat ttattttgca aatgtagcag attgaagttt tttatattag atttatagta
    31261 gataaaaaac agtttttttt ggcgcatttt tattgggcgc tcaatgcaag aagggataga
    31321 taagttcttg ctgtatttta tttttatagg agatattatg tcatttaaac attcaaaaga
    31381 aaaaaatgta gaaaaattgg atcttttttt agaagaggct attcatagta taaaaacaga
    31441 agaagacccc tttgaattaa acaatttaaa aaaagtattt aaaaagtcag ttcctttttt
    31501 tatgcgtagc tacgtagcgg tgtacatgtt aaaaaaactt acaaatgtat ccttacgaga
    31561 ggaaaaaaca agtcgcagtg cttcttctcg agaaaaaaaa gctccctaca agcgctcacg
    31621 cgaggacttt gatgaaagtt tatacgaaac acttttcttt agtgttggcc gtaaacgacg
    31681 tgtttatcct aaggatatcg ttggcttact tatgcaaaat gcaaaacttg aaaaaacaca
    31741 gattggtttg attcgagttt tggataatta ttcttttgtg caaattatga aagaagatgc
    31801 gcaaaaagtt atcgatcttt taaatggttt tgaatttaga ggaagaaagt taaccgttag
    31861 ttattctcga aaaaaagaag aggcagatag ctaagagtat ataaaaaggg aagggctatg
    31921 aaagatgtct tttgtctaac cggtggtgtc ttaggaacaa atacatatat cgtaccgctt
    31981 gcaaataatg aatgttttgt tgtagatccc gcagtcgatg cggaaaggat tgcagaaaaa
    32041 attgcaagtg cagggcttcg tttgacagca attcttttga cacatggtca ctatgaccat
    32101 atggaaagct tacaggagct ttctctgctc tatcctcaag caaaaattgc gattcacagt
    32161 gaggatgcaa aatccttggg gaaagatgcc cttcttcgcc actatgcaga gtttgcatca
    32221 actggtttag tgcaactcta tgcagccttt caaggtgact ttgaaaaaaa tcctcaccta
    32281 cctgaagcag attttttcct aaaagaagga gaagtgtttg aaaacgaatg gcttgtcctt
    32341 cacacaccag gacatacaag gggctgtatt tgcttgtata acgaaaagaa aaaacagctt
    32401 ttaagtggcg ataccctttt ttacatgggc tggggcagaa cagatttgga gggcggagac
    32461 caaagagctt tgcaaacgag tttaaaacgg ctctttgatt tgccttcaga gacaaaggtt
    32521 tttccggggc atgagcaata cggttttagc ttggcggaaa caaatgcttc tgtaacaaga
    32581 ggatttctta ccatttaatt tgtctttatc ttgattaaaa aaaaagactg ggaaggtccc
    32641 cagtcttttt ttttattttg tttcagcaag agcctctgaa atgctaaagg cataatcccc
    32701 aattttttct atatatcgta ccaaatcaat gtagagcaat tcgcttcgaa cgtctgcacc
    32761 cttttcaagc cgctttcgag caatcttttt gaggtttttt ctaaaggcat caacttggtc
    32821 ttcgagcacc tcggctaagc gcaattgatc tcgactgagg tgtttgttta tatttttttt
    32881 aataaagagc aggaattgtt gtacaagttc cccatacggt tcaagtcgct ccatatcctc
    32941 atcattaaag gtcatttctt tttctatgct tcgctttaat aaaagaagga tactaaaaca
    33001 ttcatcggta atactttcaa ggtcatcgat tatgcgtaac ataatcgaaa cattgtgctt
    33061 tgtacgatca gcgataggaa gatgtgagta ttgaataaga aagttcgaga tttcttcttg
    33121 catttgatct gcataatctt cttgctttga aagtttatcg aagtgctcat cgacaaagtc
    33181 tttcgttcga tttataaaac ctttttgaat tgttgtaaac atatcgatag caatgtcggt
    33241 catatcggca atttcttttt ctgcctgaat aatataggcc tcgtagttat ccttcatgct
    33301 cggtttaata aaggtaagcg tataaatgtc gggaacctcg ccttttttag gcttaataat
    33361 gagctcagaa agctgtgcca tctggcgggt aaagggcaaa aagataagcg agctaaagat
    33421 gttaaaaagc gtgtgcaaca ttgcaatatg aattgtgata gaattttgta caggtcccgg
    33481 aactatccaa tcggtaagtc ggagaaaggg tttaaagaaa aacattgcca gagcaaagcc
    33541 aaaagcgtta aacataacgt ggacgagggc agcacgacgg gcatttacct ttgtgccaaa
    33601 agacgccatg attgcatcga tggttgaccc gatattgctt cccaaaatca ttgcagctgc
    33661 aatttcccag cttagaagtt gttgataaga gagtgttata acaattgcgg aaactgcaga
    33721 agaagaatgc aaaagcgcag taaaaaaaat acttaagaag atggcaagaa tataactcca
    33781 tagaccgtgg ttaaaaatag attgaaaaac tgatatttgt tcaggattta cgtcaagaag
    33841 gtttttaagt aagtctaagc caaaaaataa gagagagaaa cccatgattg cttcgccgag
    33901 ggtttctttt tttaattttt taacggaacg taaaagatag ccaagtccaa agagagggat
    33961 tgctagagca gcaatattaa aattgaagcc gaaaagagcg acaatccaag cggtaacagt
    34021 cgttcctata tttgcaccga agataacacc aaccgattgt gcgagggtta tgagtcctgc
    34081 gttgacgaat gagacaacca taaccgttgt tgcccctgaa gattggatta tcattgtgac
    34141 aaaaagtcct gtcaacaagc ccataaatcg attttttgtc ataaatccga gtgctttgtg
    34201 aaggcttttt ccagtacttt tttggatacc gtctgacatg agtttcatgc caaatagcaa
    34261 gaatgataaa cttccgacta acttaaataa gatggatata actatcatgg catgagccta
    34321 tcaaaagaaa gataagtttt caagatggag aaaatgtgat agactaaagt tatgaatgta
    34381 ttaaaaaaaa caaatacagt attgtatcga ctattgtgca tattatgcct ttttatgact
    34441 tttagcttca ttggttgcag aaataatagt aaaaaaaatg caaaaaatgc tatagaaaat
    34501 acagatgctg atttgcaaaa tttgcaaaaa tggcaagaaa gtgatgagcg tatcgccctt
    34561 gttttggggc ttggctatac cgaagagcat tttgtgcatg agcttttatc ggttttagga
    34621 gatgcatttg gtctagattg cgagaatggt ctcatctacc ccctcctttt cccagacgat
    34681 tttgtctata atggcgtaaa agggcggata agttctctac ctgatatact tatggaaaaa
    34741 aaactgaggg ctgtctatat tgtgggagct ccagaaggga ctcatagggc cttagctaca
    34801 atgcaggata aaaaagaaag ggccttagat atagctgttt tttcactttt tccccaagat
    34861 gacgtcttag gcattgaatc tggctccgat atagtaatcg attttggtca gttttattct
    34921 ccttctccaa tgagcgaaga aggcggctta gaacatttga gccttgtgcc agaaatatta
    34981 cattctctca taaaaaaaga gagccttttt gatagaaatt tttcttctaa agagaaaaaa
    35041 acctttttgg aaaaaagcct tggtcttgca tggaatgttg aaactagtgt cgatttgcaa
    35101 acaggcttgc gatctaaaaa tcattttgac atttctctga tagaggagta ttctgatgct
    35161 aaggctgttg tagatagcct tataattatt gataaaagca caaataaaaa ctaaggaagt
    35221 ctgatgccag ttttacgaca agacgaatct tacctcattg gaacagcgca agcgattaaa
    35281 gcacttggaa aaaaggataa agcctacgtg cttgttgttt ctgattcaca tggtgattac
    35341 gatcgatttt atgcgctttt agaagcctat ggttcaaagc tcgatgcact tattttttgc
    35401 ggcgacggaa gctttgacct cgttggcatc attgaagcta tggcaaatga gtcaccagat
    35461 tcccctctgc ccgataaaat ccttcctccc gttatagcct ttgtccgcgg caacggggac
    35521 gcagagtcat atagctatac ttataaaggt aaaaacgaag ttattattgc gccacaagca
    35581 caaaatctca ccatctgtgg gaaaaaaatc tttgttgccc atgggcataa atacagtgtc
    35641 gactttggct tagactatgc agtagaaatt gcagaaaaac ttggactcga ttattttttc
    35701 tatggacata cccatattcc cagagagaaa aaactaaaaa acacctggtt tataaacccg
    35761 ggaagttgct cacgtccccg cggcggtttc cctccgacct gcgcactttt aaaactatac
    35821 aaagatgaaa aagaagcagc ctgtcatttt tacgaaataa aagaaaatcg ctttggaaat
    35881 gtcgacttaa agcccatgaa aatctaggcg ggggaagaaa aactccattc cttgacaatc
    35941 tttttttttc gtgatactat caaattgata tcagacagat attgttttgt agtagtacaa
    36001 tttctagttt ttttatgagg agattatcat gacagaaaaa aaagcaccct ttctaaatgg
    36061 tatggtggtt ttgcttgcga gcattatcgt atttttattt gctattggct tgattgtata
    36121 tgcgtctata gatttttcca aaaatgccat tgttttacct tttgttgttt cgattgtttt
    36181 tgggcttctt ttatccgcta ttatttgtcc gctactctgg gcaggcttgc gaattctaaa
    36241 gccgcaagaa gcgcttgtgc ttaccttatt tggtaagtat tatgggacgc tttttggcgc
    36301 tggttttttc tttatcaatc cctttgttac ggcgatgact gttggtgtag gcaatgcaag
    36361 ttctggttcc actggcaaga gtgatacaaa aacggatcta ggtcttgagg ttttgaaaag
    36421 ttttagtatg ctttctaagc caaaaaaact ttcgcttaaa gtaatgacgc tcaataacga
    36481 caagcaaaaa ataaatgatg cgcttggaaa tcccatcgtt gttggcgttg ttgttgtatg
    36541 gaagataatc gataccgcaa aggcaatgtt caatgtcgat aactacgttg aatatttatc
    36601 gatacaatgt gatgcagcgc ttcgaaatat tgttcgattg tatccttacg ataataccga
    36661 agatgatggt gaaaaatctt tgcgtggaag tagccttgaa gtcgcagaaa aattgaaaga
    36721 agccttgcgg gaaaaggttg ctgttgctgg gcttgaaatt gttgaaacaa atattacgca
    36781 tctttcgtat gccgcagaaa ttgcacctgc aatgctgcaa aggcaacaag cggttgctgt
    36841 tgttgctgct cgccaataca tcgtcgactc tgcggttggc atggtcgaaa tggcgctcga
    36901 taagctgaac aaaaagaata tcgtagcgct cgatgaagaa agaaaggctg caatggttag
    36961 caacttaatg gtagtgcttt gtgcgggcaa agaagcgcaa cccattgtaa atgcaggaac
    37021 actttattag aggcagtgtg ttataaataa tggctggaaa aaaaacctta gagatagata
    37081 taaaagaaaa tacgcaagaa aaaaaacaag tgcttttgcg tatttctgct cgcttgtgga
    37141 atgatttagc tggctgggca gaagatgatt ttcgttctat caatgggcaa atagagtttt
    37201 tattgacaga agcggttaaa aaacgaaaaa ataaaaaaga ctagttttta ttaaatagtt
    37261 cttgacaata gaaacttagt gtactattgt gatgatacat tgacgagata ctgtcaaaga
    37321 tcttttacac tcgtcatttg tggcgaaaga atttttataa ataggagaat gtatggcaat
    37381 tattagtttg gaaaatgtgc gcaaggtata tcccttggga aaggttgagg tggaggctgt
    37441 tcgaggtgct tctcttgaaa tagaaaaggg aagttttgta tccatcgcgg ggccttctgg
    37501 ttcgggtaag tctaccatct taaatatgat tggcttgatc gacactccca gttttggagc
    37561 tgtcaaaatt gacgggcatg atgtctatgc cgggcgtttt acgagtgata ttaaaaagaa
    37621 aaagaaagat atcctcccat caaagatgga taataaaata acagatttac gtcatgaata
    37681 tctcggcttt atctttcaaa attttaacct cattcctgta ttgaatgtct atgaaaatat
    37741 tgagtttcct cttttactgg ggaaacaaaa gatgtcaaaa aaagaaagaa aggaatggat
    37801 cgattattta atcgaaaagg ttggtttaac cgcttgggcg catcataagt caaatgaact
    37861 ttctggaggg cagaggcagc gtgttgctat cgcccgtgct cttgtaacaa aggctccggt
    37921 tattttggcg gatgagccga cggcaaatct tgattcaaaa acaggcgatc aaattttaaa
    37981 tcttatgaaa gaaatcaatc gtgatttgca aacaactttt attttttcaa ctcacgatgc
    38041 aaaaattacc gcaatggcag gacatgttat tcgattgctt gatggcgaca ttgtagaaaa
    38101 caccaaaata gaaaatggaa gtacgaacaa tgatgctgtg cagcttgcat gtaaaaaaga
    38161 aaaggcacag gctgaaagag aaaaacaagt tcttccaagt gatactatgt cgaaagacct
    38221 cggcgtttag tttaaggaag gcggtagaga tatgaaaaat acgatacgaa tagcgcttgg
    38281 caatttgtgg gagcataagg cgaagacgct catccttgga ctcctcgttt gctttggctt
    38341 aatgctcatt gttttgggaa caggtttttt agaagcggca aaaaagggca ttgaaaaaga
    38401 ttttcgctct cactatacgg gtgatattgt tatccacgga ccgagtccta aaaaaggtgt
    38461 gcttacctta tttggcgtgc aagaaaatgt gactgtcggt gaaatggcaa agactccagc
    38521 tcttcctgat gcagaagcac ttttatccct ccttgataca gtcgacggga tacaggatag
    38581 gacgagactt atatcgggca tgatactact cagctctgat gcaattggcg aaggttttat
    38641 tccacgcaca gaagatgagt catcatttcc tatgggattt tttctttcgg gagacgagaa
    38701 ttattttagt atgtttccag atgtaaaaat aatcgaaggt gaaatgtata atcgaaatga
    38761 accttcatta attatggacc tgagtttaag ggaaaagttt gaaacctatt atcgcattcc
    38821 cttagcagtt ggcgatatga ttaccttaca gggatatagt gcggcgagtg caaaactacg
    38881 tgaagtaaag attgctggtt tttttgcgca tcctgatcca aatacaacga tgatgagttt
    38941 gtgctatctc gaccaaaata ctgcacgtgc cttgggcgac ttaacctacg gttctgttta
    39001 cgctcgtgat ttaccacagg tgatcgatac aagtctttct acaatggatg aagatgattt
    39061 atttgcagat atgtttgaag ttgaaaatgc ttctatttta ggctcaagct taacggacga
    39121 tgatgttgat tctctcttgg gagatacaag tcttcgcgat aaactgaatg aagtggcaga
    39181 tggcgcttgg caatttgttg tattaaaact ggatcgccct gaagaggcaa gtatgaagat
    39241 aatagaactc aatgctttgt ttgcagaaaa ggaaatgaat gttaaagcca tcgactggaa
    39301 gactgcttcc tcaggttttg ctatgggaac tgatattctc ggagctgttt ttacaatttt
    39361 gattgtgctt ctctccgttg ttgtttttat cgttatcatg aacacactta ttatatctgt
    39421 tatagaacga acaagtgaaa taggtacaat gcgagctctc ggtgcacatc gcggatttat
    39481 tcgaaaatta atttttaccg aaacaacatg tattgttata attgcttctc ttgcaggcat
    39541 cgtccttgct ctcatcattt ctggcattat caatataagc aatatacgta taacaaacga
    39601 aacagcaaaa ttatttttag gtgggggtag ttttaaaatt acaccaaatc ttggttctac
    39661 actggctgcc ttctttgcaa tttttatcgg cgcaagttta gcaaacctct atccggtttc
    39721 ctttgcctta aaaattacgc cactcaaggc aatgaaccaa gaagactagg ggggattatg
    39781 aaaacaatag gattagcatt tagaaatttg agtaggcaaa aaaaacgaag cttcttactc
    39841 ggtggcgcca ttgcatttgg tttttttatt gtaacatttg tagatgcctt ggcaggtggt
    39901 tctttgagaa acttaagcga gcaattttcg tttttgtttg gtggaaacat ttttatccaa
    39961 gcctctcaaa aagacgatga caaagttatg actgtaacaa aagatgcgct ttttatacaa
    40021 gatgttttga aggaatctgg cattcggtat acaactgagg gaaaacgaac gatgctgttc
    40081 ggcacgatta tctatgaagg gcaaaaggct gttacaaata tttctggttg tgattttgaa
    40141 aaagagaagc gtttaaaaga ttctatttct cttgtgcaag gttcatggga aaatatgcaa
    40201 gatgcaaatg cgctcattat aggtgagaca atggctgaga acttaggaat cgaggttgga
    40261 gagaccgtgc tttttgaaat gcaaacggct aaagattatg caaccgtcgg cgagtttaaa
    40321 attgcggcaa taaatcggga tccaagtttg ctcggttcaa tcatggtata ttctcatatc
    40381 gcatacttaa acgaattgta tgagcttgat gaagatgcat ttttctttta tacaataatg
    40441 gcggaggatc atgaaaatca agatgccttg gcaaatcagc ttgaaacaat tataaaaaaa
    40501 tacaagccgg ttacgagccg acttgaagcc gttaagaaaa atcctttgaa tattgtgcga
    40561 ggaattaacg agcaattaaa agaggcaagt tgggaaggcg atatgtattc tgtcataacc
    40621 tttgacgatc aagtgccaaa tataaaacaa attctttcgg ttgtgcagta tgtgagcctt
    40681 ggaattctcg tcgctctctt tttagttatt atgataggta taagcaatac ctataaaatg
    40741 gttatttttg aacgcataaa agaaatcggt acaatgcgtg cgcttggtgt aaaacaaaaa
    40801 gaagcagggc gtatttttat gtgggaagct gttctcttaa gcctttttgg ttcccttgtc
    40861 ggcttcattg tcgcaattat agggatgaac attttatctt tatttacttt tacgagtgaa
    40921 gaattatccc tctttctcca caatggacat tggacctatg tgcttaatgc aggaagcata
    40981 agtattaaat tgatagttgt agtgctctta actatgcttg cagtacgagg ttcagcgaaa
    41041 caagctgcga agctcaaccc tgccgaagca ttgagaacta cgagatgagg aaaaatatga
    41101 aaaaaataat ttttattgta ctaagtatct ttttattgtt ttctgtaatg gcagaaaata
    41161 caccagaccc ctatgcaatt cttgatgtat tgcaaaaaca atattcatat gacgaccaag
    41221 attattctgc gactgttacg ctcattgttg aaaagccaaa tcaaccagct gaacaattac
    41281 agtttaagct ttttcaacgg gcagcaaaaa aacaatttac gatgattcaa cttttaccag
    41341 agaccgacaa gggtgctgga tatttgcaag atggagataa tctttggtat tacgatccta
    41401 tcgggagaaa atttaatcat acttccctca aacaaaacat tggaaactcg gatgcgaagg
    41461 caggagatgt ggtaaaagag tattcatttc gcgatgatta tgaggcaaca agcattgaaa
    41521 gcggaaagct cggtgccttc gatgtatgga taataagctt gaaagctatt tcttctcgtc
    41581 ccgcctacgc aaaagaagtg tattatataa ggaaggatat tacgctttta ttgaaggaag
    41641 aaaattatag cgcaaacgat agacttatga ggacaattct ctatccaaaa tatacaagag
    41701 tcttagaccg ctatcttccc atacaaatta ttatgcgtga tgaagtaaat aagggagagc
    41761 aaacgcaaca aatcctctca gagatttcga cgagcgctat tccaaacaaa gtttttacaa
    41821 aggcctatct cgaaggttta aattaggtgc ggcatgaata aaaaatattt ctataaacag
    41881 atttttattt tttgccttgt ttttttcctt ctcggtataa aagcttttgc tattgattca
    41941 gatgactatc tattttcaga tgatagtttt tctgacgaag gtttttttag cgatgcagag
    42001 ttcttttctg atgaagattt gttttcggat gatatttttt caagcgagac ccttgtcgaa
    42061 acaccagtaa caaagatcga cgcaagtctt tcttccctta tttttgaaac aggctctgtc
    42121 cgctttgggg gaagcttgag tgcaggactt cacatggaaa gcatttggat tgaccccttt
    42181 agcagtgatt gtgattttct atatggaaca gaaatgggag ctataaaact tggtggctta
    42241 gaaaacacag gaataaatcc tacgctagac cttgatcttt tttttgacag ccgtccttcg
    42301 agcaatacaa gactctatgg caagttcaat gtcaattatc cctttaatat atttgtagac
    42361 acggctctct ttcctttacc agaagcctat gtactaacag aaattatagt tcctaacttt
    42421 tctgtctttg agctttttac agacttcaac tacgatgaca aaatctattt tcgttttggc
    42481 aagcatacgg taaagtgggg agtggcgtat ttttttagtc ctgccgatat tatcaatctt
    42541 gcagctatcg atccagaaga tccagaaaaa caacgagaag gacccatttc tttgagagtg
    42601 cattttccta tcgagggaac acaaacaaat ctttggtttt atatcttacc cgatgaaaaa
    42661 accatgcttg taaaagatag tagttttgca tttaaagcgg agtttttagt tgctacaagt
    42721 gagcttggtt ttggcgcttg gtatagatac aatagcccac cgcatcttat gggaaccttt
    42781 acgggaagcc ttgctggaaa ggtggcactt tttgcagaaa cagtttttgc ttggggcaat
    42841 gaagacgcat ggcaaaataa cagagtcttt actgagatga aaccagtagt agatgcaaca
    42901 cttggggctt cgtattattg ggaggcagca aaaataaatc ttgcaggcca atatttgttt
    42961 ggctttgatt tttcaaatct tgatacaaag aattatactg gagcgcacca agccgcattc
    43021 tctctttcac gttcagagtt tattacaaaa aaaatgtcgc tttctgcttt tactctgatg
    43081 gatatacttg aacataccgg cacagcgagt ctcaatcttg gagtggattt ttttgatggc
    43141 ttttctttaa gtgcaggacc agctctttca cgatttgcca cggatagtga aaagaaaata
    43201 agtgcatctt tttctctttc tttacgtttc ggaggaggtc gcttctagac cttccctttt
    43261 taattcgata aaataaggac gataaaaagt gcttataaaa aatgaggtta gataaaatgt
    43321 tagaagcaaa aaaacttaca aaaaactacg gtgatttttg tgctgtcgat gcgcttgact
    43381 ttaaggcaga taggggcaaa atcgtcggtt taattggacc aaatggtgcg ggaaagtcca
    43441 caactattcg catgattatg aatattcttg atgtagacag tggagagatt gtacttgatg
    43501 ggcaaaaatt taccgcaagc gattctgata agattggcta tctcccagaa gagcggggac
    43561 tgtataaaaa acaaaaagtc atcgatgttt tatatttttt agcagaatta aagagctgct
    43621 caaaaaaaga tgcccttcca aaaattgatt attggcttga gcgttttaac ttgctcgagt
    43681 ggaaagacaa aaaaatcgat gccctttcaa agggaatggc acaaaaaatt cagtttgttt
    43741 cgactattgt gcatgagcca cagattttgt ttttagacga acctttttct ggcttagatc
    43801 ccgtaagttc tgatgagctt ttatccgtga tgaatgaatt aaaaaacgca ggtcggatta
    43861 ttcttttttc aacgcatgtt atggaacaag ccgaacgagt ttgtgatcaa attatcatgc
    43921 ttgatcgtgg gaaaaaaata ttggacggtt ccgtctcaca aataaaaaaa caacagggaa
    43981 gcgatagctt ttcaattcgc ctcgatggag atgcttcttt tatagaagaa ctttctattg
    44041 tagataggtt tagcttaaaa gaaaaagaga cctgccttgt ttcattaaaa acagggacag
    44101 acgcaaacga cttgttttct gtcatcgcaa gtgaatcaaa aaagcaaaat aaaacaatag
    44161 aatctattac acgtaatgag ccaagcttgc atgatatttt tgtaaaaatt gcgggtaaat
    44221 caatcgatga atctggagat gtaaaaaatg aaaaataaaa ccctcgttgt attaaaacat
    44281 gaatttaata aaattgtaaa aacaaagacc tttattatta tgacaatact cggtcctttt
    44341 ttacttgcgg cgattatgat tgtacccatg tatttttcta tgaaatctgt agaaggaaaa
    44401 gctgaaagtt taaaaatagg tatatatctt gccgatagtt ctcaagaagt tttagcagaa
    44461 gtcatagtag aagaatcaac tgctttaggc tgggagcttt ttataagcaa agacagagaa
    44521 agtctctata cacaagcgat aaaaaaagaa attgcaggtt atcttacaat tgaagaagat
    44581 gcaataaagt actttacttt gaagccagga gactttaccg tcaatgcatc cttgcaatcg
    44641 attatatcag attatctcat cgatgaacga ctcagtgctc ggggcttaag ttacgaagaa
    44701 gtaaaagctt taacccaagc atactccttg cctgtgttta aaatctcgga agataacgag
    44761 acaggggtag aaagtgatga gggaatggaa tttgcccttg caattataat tcctatgatt
    44821 ttttctctta tgatttacat gtctgtttta ttgtatgggc agatgatagg tcgatctgtt
    44881 gtaacagaaa aatcatcaaa ggtcgttgat gtgcttttat cttcagtaaa accgaatcaa
    44941 cttatgatgg gaaaaatttt tggggtaggg ctagcaggaa tgctacagta tagcatttgg
    45001 atttttatgg cccttgttgc cctctttgta acaaaacttg tttttggatt tgctattcct
    45061 gcacaaattg ctatgagcaa ctttgtgtat cttggaatct attttgtttt aggctatctt
    45121 ttatatggct cactttttgc agccgttggt tcagcttctg aagacgaaca acacctcggt
    45181 caactctctt tacccctcat acttttttta gttgtgccta ttatgctcat ctcagcaatt
    45241 atcgaaaatc cgaattcgac cctttccttg gtctttggct ttttcccact caccgcacct
    45301 ctcgttatgc tttcacgcat tacaacaggt gaaataccgc tttggcaaat tcccctaagc
    45361 attggaagtt tagctctttg tatttggttt tttattaggc tttccgcaaa gatttttaaa
    45421 acaggtattt taatgagcgg aaaaaacttt aagcttcgtg aaattgtaaa gtggttacgc
    45481 taactttcaa agagagcttt tgcgaacaat tctggttggt gaaagctctc cttttctgaa
    45541 gaagcgagaa gtgtttgata gccctggatg ccatttcctg ggttatcgat tctattgcag
    45601 gtgaatgacc agattttttt atccaaactt gtataatcaa aatctctgag tccaaaatca
    45661 ctccacggaa taaaaatttc ggcataccaa aactcatctt ctatggtatt tgatatgaga
    45721 gcctttgatt tccagctata atcaaaaaac aatatttcat caccgttttt agcttcacta
    45781 caccccgaaa caatagcatc tgtatcctgt actatttttt tgtttttttc atctatacta
    45841 tccaatataa gctgcttccc tttttcacct attccacaac gataatcaag caaaaatcct
    45901 tttgagttaa attcaaaacc ataatagata tcagattctt ctggacgaat aaaaaactcc
    45961 aaacaatcat cttcgaaaac acgcattttg tgggatgttt ttttagaaag cagtttgttt
    46021 tttggcgctt cagcacaaaa agaaactgcg ataccatcta ttccggctgc aataaaagca
    46081 agcgtaaaaa ggcttcgggg tttatgcaat ggccaaggat aaaaaagctc aattccttca
    46141 acctcataac tcgccataat tgcatcagca ttttctgaaa gattttctga taataaggga
    46201 atataatatt gtctcatgct tatattctaa agcttttctt gcaggactgc aagttgtggc
    46261 ttgatttttt ctggtatatt ggtatgctag aatgaagaga ataaaaaaag aagcggctta
    46321 gactgtcttt ttgttttatc gtgaaaatga tttttctagg aggatgatat gtcaatatgt
    46381 ttaactaatg gcaccttgat aagcgggtat tatgcgcttg aaaaaatgag catccttatt
    46441 gaagatggca aggtcgttga cgtttttaat caaaaacgtt ttgagcaaaa aaaatttcct
    46501 ccgacagtgc gtattatcga tgttgaaggt tcttttattt ctcccggttt aatagatact
    46561 catatccacg gctttaacgg gcatggtacc gacaattgta gtcctgaggc gatgcttgcc
    46621 atgtctgaag accttgcaca atatggagtt accgcattta acccaacctt atatccatcg
    46681 agcgaagagt ctatgacaaa aacaatacaa tctatcgtaa aggctatggg aaaggaaaac
    46741 ggtgcaaaaa taatgggcat tcaccttgaa ggacccttta tttcagcaga taagcttggt
    46801 gtgcaaagac ccgaaacggt aaaaacagtc gatttagaac ttatggaacg attgtggcaa
    46861 gcatcagaag ggcatattgt taatatgacg gttgcaccag aaataaaagg tatgcgggat
    46921 cttgccttat attgtataaa aaaaggtatt gtcgctcaag caggccatac gaatgctgaa
    46981 tacgcgcata tggttgaagg aatgcaagcg ggaatcttac acgcaaccca cctttttaat
    47041 gccatgagtc agatgcacca ccgaaaccct ggtgctgtag gggctgtttt aattcaccct
    47101 gaaatgtcat gtgaaattat tcccgatggc attcacgtgc atcctgattt gttaaagttt
    47161 gttgctcgag ataaaccaat tgataaaata gttttagtta ccgatgccct tactccaaca
    47221 gaacaatctg gctctcccct gtatgctaac ggtgaagagg ttactttttc aggagggtgt
    47281 tttcatcgag tgaaagatga cgtcatcgca ggttctggtc ttactatgat aaagggtgtc
    47341 aaaaaccttg ttgcctatgg tttttccgta gaaaaagcaa ttcgttgtgg taccgcaaat
    47401 ccagccgaaa ttatgaaata cggtactaag ggtgttgttt ctcctggttc tgatgctgat
    47461 attattgtat ttgacaaaca ttttaatgtt ttattgagta tggtcgaagg aaagattgtt
    47521 aaaaatctca tttgatagta gaatagcact gtgatacatt ttttttggag gtataaatga
    47581 gagtaatagt acagcccgat tacgaggctt gttccctttg ggctgcaaat catattgcac
    47641 gtagaattaa cgaggcagct ccaactgcaa aaaaaccctt tgtattgggt ttaccaacag
    47701 gcggtactcc tattggtact tatagggcac ttattgcatt gaataaagct gggaaagtgt
    47761 catttgagca tgttgttaca ttcaacatgg acgaatatgt tgggcttcct tcagatcacc
    47821 cacaaagcta tcatcgtttt atgtgggata atttttttaa tcatgtaaat attaaaaaag
    47881 aaaatgtaca tatcctcaac ggaatggcac aagacttgga tgcagaatgt gaagcctatg
//
LOCUS       DAOSSW010000004        45688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_915_length_45688_cov_7.320207, whole genome shotgun sequence.
ACCESSION   DAOSSW010000004 DAOSSW010000000
VERSION     DAOSSW010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 45688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45688
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_915_length_45688_cov_7.320207"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1790)
                     /gene="uvrC"
                     /locus_tag="PKW26_00765"
     CDS             complement(60..1790)
                     /gene="uvrC"
                     /locus_tag="PKW26_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOQ92130.1"
                     /translation="MNTIKSLHETALKAPPLSGVYLWKNAEEKVLYVGKAKNLKNRLK
                     SYFSGKKDIKTRTLLSRAAKIETIICSNEYEALILENTLIKQYSPQYNISLKDDKSYP
                     LIRITNEDFPRIFKTRRIVKDGSLYFGPYPNGTALQTFLELIYKNYPLRRCKTLRKRK
                     SPCLYYHIGRCSAPCCDYISKEAYRLYIDEITAFLEGKPSETLEKISVLMKEASKELN
                     FEKAAQYRDLHDSLSHLLRTNLVTDSIFEDRDYIHFAFDQSFVQASILKMRDGKMVEK
                     DSLRVKSIHNHHDAGSLKDLAEEFLRSYYEEKDAPQYIYLPIAIDTKLYSEYLTKTKK
                     TAIQVLSLVPKAGEVAKNLTDEEKSEEGELAVAFDKPHYDAQSRHRAILDLAEQNAKE
                     DLRKRLSERGDFAGMEDLKEILKLEKLPLVIEGFDISHLDGHFTVASMVQFYRGKSHK
                     KEYRYFTIKSLEKGKIDDYHSLKEASARRYSRLLNENLPFPDLILIDGGKGQLHAVEG
                     ILKALEIDIPLIALAEKNEEIYLQDKNEPLRLPSNSPARLLLQRIRDEAHRFAQKNVH
                     ASQKRQLRGE"
     gene            1799..3295
                     /locus_tag="PKW26_00770"
     CDS             1799..3295
                     /locus_tag="PKW26_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92131.1"
                     /translation="MIIANKKNIVIFLIGFCFAFLLPVFSEEESIQIGGKNGWQNLLV
                     SKNIVSTKGLFGYEAKALAKKERAHTESLDLLLSFDGSDIKDTLDRYRVVNEDSVKTY
                     RKSSDAKTGTAALLSLEKKPLVLYGRGDAFFASSGYKGSFSISFWIKPFLLQSQERLF
                     SWNSSRNTLNTVLDQSIQAFFNKGRLQWQLSNIFTTSEAIETDFLLKGSSMLIPNSWS
                     HHSFVYSEADETIEYYVNGFLEDVLSVKTLKPYLGKQADIRLCENYTGLLDAFVIDND
                     DAVLNGDGAVYSKERGRFETQPIDLGTYGTVVRKLEAEVDIPSQTAVAFFLRSSDNFY
                     EWTETYPAWQPVAPGKQLDGFIGRYVQVAAELYTDGAGKKTPAITDISLFYQKTEAPL
                     APYMLKAEGGDGYIDLSWAKTLDENTAGYLIYYGESSGEYLGTFALEGNSPIQTELQE
                     KFRIRGLENGKIYYFAIRAYSAVDERIIGPFSKEIWARPRENKGAFYE"
     assembly_gap    3320..3419
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3420..5942
                     /locus_tag="PKW26_00775"
     CDS             <3420..5942
                     /locus_tag="PKW26_00775"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ92132.1"
                     /translation="LMGNNEEGAKCFQRVIDENPNDVLAYNHLGSIQAARGEHAKALN
                     TYAKALQLDKNHPILHYNAAKSYIALGNLSKAKNSYENALRAHPGWIDAMDAYARLLM
                     KMNLLPEAEDILLQAQRLDKDNLAIKNALGLAYFKRERYTEANRLFNDVLRENKKDVS
                     ALLGKSAILSKTRKMDAASLILSDLEKKYPDNREVRFQYSDLLVRMNKLVEAVKRINH
                     LYAENPNDPDLLNLISQYAIRKGEEERVQDFVDKIAQNNPAYTKHFFDIADVYFEMQK
                     LDKAEYFIRSYLEKQKKDSKAYVLMGKIYEVLKNYRKALEHYKKGVDLNPYSKEAETL
                     LKRVEPFVYNSEEKKEEVLDENKPASEYLGSFNIDLDEEDDFLFAAGEGLGDVLDEGD
                     QALADTEAVDNEETIITDVEPTSEDFFSEVPLALDEEALFEEPLDFDFPFEDEDLVDD
                     NDDVITIEDLIDTDAPFDENPALFDDPLFDDFGSSPTQYLPDEEAEVMDILDTPQGFT
                     EDSENDKALPKQEEFFSPPPLAARPQAYPPYEEKMEIPQQQTSPMHEKEKEIWEDDPL
                     LEERDEEVFLSDESMHAKEEAARKPEEKRDRRKSDTELEKLKEDYDSLGNRFDKMRDR
                     YDKLRDSQDELRNRFSQLEDNYDDLDDKYAYLSQDADLIEDIKEDKGLSESDEIKGRE
                     EAFFVDTEPGLDEDLPVETKLPDKNINEDEIFSVNTGKEEEEGNSEQAGIPSLFENLE
                     DLCTYLPEKKKLDFLSSIERLKMHYLMQKMLGGKGLLLKSEDVRNEAGIAVDQLDDLD
                     ADSVLMLFFHLQNLTTELRDKQLGALLYEKLSEIKEACSAMW"
     gene            complement(5959..7083)
                     /locus_tag="PKW26_00780"
     CDS             complement(5959..7083)
                     /locus_tag="PKW26_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOQ92133.1"
                     /translation="MNEIYKARRQKLADWMSLNDISAVIFVDNEEKRSPEIRYFTNHA
                     EDALLIITKKGSSILCPWDENLAKEKAHVDLIIPYTKYELKPIKASLALLKTLKVPGK
                     TRVEIPSSTSYPEFLRYVDALADYDVLCNENFAHQFVTNQRQVKDAAEIKKARKAAEI
                     TNTIIDAIAEALKKNAFKTEMDVALFIERSCREHGCEGTSFPSLVANPSRSFAIHCFP
                     NYTSGNFPASGLSILDFGVNYDGYASDVTLTIAKGSLSAEQEKQISLVEKAYDLALPF
                     YAEGTPVKTAAVKVDEFFAKAKRKMPHSLGHGMGLQIHEAPWIRTKTSSSAVFKVGMI
                     ASLEPGLYDPKVGGCRLENTILITETGPEPLTRSRVIRLP"
     gene            complement(7093..8703)
                     /gene="murJ"
                     /locus_tag="PKW26_00785"
     CDS             complement(7093..8703)
                     /gene="murJ"
                     /locus_tag="PKW26_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOQ92134.1"
                     /translation="MVDAKTKKKNILASGLSLSFFTLISRILGLIREITKAALMGTSQ
                     LSDAFGVAFMLPNLFRRLFAENSISVAFIPTFRSYLDDDKKEKSEIQTFLSATLSLVS
                     LATTITVILGIIFAPQIAVLFSKHNSADIISETALLTRIMFPYLFVISIAAFFQGILN
                     AHHIFSPSGFTPILFNIIVIASSYLLIDYAGNPARAMAIGVILGGTVQALFQLPFVLK
                     QGWKISLTGLKKTLSNPGTKRVLRLIAPTIIGMAAYQLNDFVSSIIAKNTGIGILSSI
                     QYSLRLQELILGIFAVTVGTIILPDLSSLAKREKWQDYNQMLLSALKLIALITIPITF
                     FSILFRENLIILIYKTNQFSDESVALTSGVFFFHIIGLFCIAANRILSPAFYAQSNAK
                     LPTYAGIIGFACNIILALILSRSMGGNGIALALSIASFINSIALLFFLHRLSHMQAVF
                     LTKQIIGYTIKLALLSLIAIAPVYLIKNRLLSIFSGYNKLIAQAVPMAIATLIFFSLG
                     ILLLLLTKDSLLLSIAKKIKQKIFQNGR"
     gene            8790..10475
                     /locus_tag="PKW26_00790"
     CDS             8790..10475
                     /locus_tag="PKW26_00790"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOQ92135.1"
                     /translation="MKKGLFFLKVGKTRIIPLSVKLVSVFVVLLLLSNFATNFINLQM
                     NQRQITILSNKILVSQLKDIYTVVQNQYKVYEFSKDIDSSLQAIEMVSRNALSYKNSI
                     VMGARPGASSFFSVAYEDPFIRFPDELLLLEMKDSLSQGIDEGSIRFSSSKGNYFGVY
                     KYSPEWNVFIIRADLIADVEKSTNRNFIIISAIILLFTILFLFAGLVMFSKILASIST
                     ITKSLYKMQQEQKLSLLDLSNSPNDDISYLAASFNSLSTTINNLLGIFQKFVSKDVVE
                     AAYKEHSIRLEGSQKDLAILFSDIRGFTYMTETLGNDIINLLNIHYDKAIAAIHEENG
                     IIGSIIGDAILSVYGAMGSYKNNSLEALRSAWQITRVTASLREQMIERRKEIEKQRPL
                     TEAEERVFQAVLIDVGVGLDGGKVFYGNIGSFERMTNTVIGDNVNSAARLEGLTRLYK
                     VPVIASDYIKEGVESVTSQFTFIELDTVQVKGKTEGKKIYFPLDSLVTDAETLEKYAV
                     FSEALSFYYKGDWTEARKLFRASKLELARVFLDRITIKQAPKDWSGIWEMTTK"
     gene            10454..11938
                     /locus_tag="PKW26_00795"
     CDS             10454..11938
                     /locus_tag="PKW26_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92136.1"
                     /translation="MGNDDKMIIHKPELKLFLTDKMYPETKTESEHYDLENEYEKTKQ
                     NKNHTVLLMLLALVALVVGISYGITAFIAYQNKKIDVDINVFADTNLIELFDMVSRTE
                     DALASAQSEKARIEMLRQTELQEIEAKKEGELYALQSMRLTRAAQNQQEKEIQGVYEA
                     ALLDIQESYDRDLAVLEKSIEEYRKQLETLDSSRVKQAQEQQVALDFQRHIYEMEKVQ
                     LQEKYELIIQDLREKIEEQQRLAYERQMASIDSLSSDYQRLIALLDPIVEDPRVDEIK
                     AEIAEFVEMRAFYADDYRISAKNEVYNHLLEKIEHAYTSIDYLAEIVSAIPYENSIPS
                     ILETIESIYYVIGIELAYFSSNEIASLQKSLDTVHADYAAEIAALESSHKKNLENQKL
                     SFQNQLADQKDTYEKKLIAQANEFDKIVENSRSYLLGLERELSKNRTYIEALEKENRD
                     LKENQKQIETASEFSVFIDLEEDDLKEYEDDREDKKETIQTLIE"
     gene            12046..16488
                     /locus_tag="PKW26_00800"
     CDS             12046..16488
                     /locus_tag="PKW26_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOQ92137.1"
                     /translation="MSKIDTVDFLTGSEPLRVGIDVGSTTVKIVILGENDTILFSKYE
                     RHRADIRTTIIAVVNEALDVAEKTYPEGKEQLLSVKVTGSGGLAVSHWLGIDFIQEVV
                     ASTRAVQKLIPQTDVVIELGGEDAKITYFSGTVEQRMNGTCAGGTGAFIDQMAALLET
                     DAGGLNELARGATMIYPIAARCGVFAKTDVQPLINEGARREDIAASIFQAVVSQTISG
                     LACGKPIRGNVAFLGGPLYFLDQLRHRFVETLKLEGEAIIAPEGSQLYVAAGAAFSAE
                     RDLSQIVPGEKSPFVSIGNLRENLTKLVGAEMTEVQRLEPLFKNEAELEAFHKRHAQE
                     IAMTYELDKSQGPVFLGLDAGSTTTKAVLIDEKGRILWRFYDVNAGNPVELAVRVLKD
                     LYKKLPKDVYIARTVSTGYGEALFQAALKADAGEVETIAHYRAAEFFVPGVEFLLDIG
                     GQDMKCLRMKNGAITSIQLNEACSSGCGSFLDNFARSLDMDIQSFSQKALLAEKPVDL
                     GSRCTVFMNSRVKQAQKEGATVADISAGLSYSVIKNALFKVIKLRKASDIGTKVVVQG
                     GTFNNDAVLRAFELVSGVEVFRPDVAGLMGAYGAALIAKDQWSDLIAIKPGEDFILDK
                     EPVLSSLASLEELESFKVDLELTRCGKCNNNCLLTINTFYTGAEGRSFVTGNRCERGA
                     ELEGGKTTVDATNAKKNALGGKEDGPLPNLFEWKYNRLFRHYKPLLKKDAPRGEIGIP
                     RVLNTYENYPLWFTFFTKLGFRVRLSPRSTRDIYELGLESIPSESVCYPGKISHGHIE
                     ALLKEGVNRIFYPCAPYEQKEDEGAGNHYNCPIVTSYPEVLRNNIDALRQDDSILFLN
                     PFLPIDHKERLKERLFEELGEILSLSQAEINKAVDAAWKEQDTFKKEVQEAGEDAIFN
                     IIRRGGMGIVLAGRPYHLDPEIHHGIPELITGLGVAVLTEDSVAHLGKIERPLRIVDQ
                     WTYHNRLYRAAAFVADMPNLEMLQLTSFGCGLDAVTSDQVEEILAAKNRMYTLIKIDE
                     GSNLGAVRIRVRSLIAAVREQERNPKKKKKKISAAYQREVFTKEMKATHTILAPQMSP
                     IHFRLIQKAFEYSGFNFVILPDVDTEAVETGLQFVNNDACYPSILVAGQMIAALRSGK
                     YDLDHVSLLITQTGGGCRATNYIGFIRRALADAGWEHIPVISLNAVGLESNPGFKIGF
                     PLLHRAFMALMLGDLLMRVLYRTRPYEAKPGSANALYEKWNRHIQKQLKSMSILAYHR
                     MIKHIVRDFDTLPLSPIKKPRVGVVGEILVKFHPTANNDIFSTIEQEGAECVVPDLAD
                     FLFYTFATGIYRYEKLSFPKKTKRNAKLLVWGMELYRKRIKKELSRSRRFNPPTSIYD
                     LMESVDDIVQLGNITGEGWFLTAEMVELIHDGVPNIACVQPFACLPNHVTGKGMIKEL
                     RRRYPESNISAIDYDPGSSEVNQLNRLKLLLANAKPGRHPDEKK"
     gene            16485..17855
                     /locus_tag="PKW26_00805"
     CDS             16485..17855
                     /locus_tag="PKW26_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92138.1"
                     /translation="MKRRQICFSIVFLFFSVGFPVYAAFNPLSISDSSSIREDIYYTW
                     LTRSLDDLREEKAIIRKNEAGDYFRVSFVEYEKEFAIICNPMVENDFPEKTSGSWILY
                     RDKETEKALRIEFYFTQSSEVYFTFKPSGTKTSIDLRIFNAYLIKDHNFSLTFRDFYT
                     LSIPQFYTLSKNLFPWHFFTPEEKLYDANIQIASQLREYQDMIDFVPNRAYDENGKPS
                     QIFDSSENLQLNTRYNVESNEILEDSDKLQLGSLGYVKWLVDGLVYPQIASYLKIQAL
                     KKATVDTSPRSRVETLSSRETIYAALDWTRNLATAYASVQLGRDLDFFTSGTEVNHSF
                     FSLSFDSHGKVRDSAYVKNSGYSSHILKPLLYMLALTEPERMYIAAIKDFRSDTEEGK
                     LDIGRFRDALIFLPYFDAKREFQCPVFYAGKEINLDELIANSENDFFSLVRIKTELRF
                     YPLKKE"
     gene            17890..18558
                     /locus_tag="PKW26_00810"
     CDS             17890..18558
                     /locus_tag="PKW26_00810"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOQ92139.1"
                     /translation="MKEIKAIAFDIDGTLYPNWRLNIRVLPYFLKNIRFFLAFKKVRD
                     ILHQTAPLGDFFEYQAQLLCDVMKCDCKSAKEYIHEIVYDGLAPYFTEIKPFKNAIES
                     FAVFKKAGFKCAVLSDFPPEQKGDIWGAKQYCDVILGSEEIGALKPSIHTFGVLSEKL
                     GLEPHEILYVGNNIKADIIGARNAGMKTAYLLPFWRRILGLKLPQADISFKNYRQLQK
                     IVLN"
     gene            18589..>21388
                     /locus_tag="PKW26_00815"
     CDS             18589..>21388
                     /locus_tag="PKW26_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92140.1"
                     /translation="MIFNIVTIGICVTLILAFRQLDKKDKSLEKVRKYSEKIIGDFEA
                     YFTQKKDNLKEASLEIDTKQSHALASVKRLDSIIEEFNKKIAAQTSLVKTLEQLEQKT
                     AHYDALIKELLEMSERVEENLMKIKAESAFIAKTQDQVHALKKQQDYLEKRIPQLEKE
                     FTAENEASLDALTKGLLDTFHKDIETIEISTRDALMQNEKILTEINQSYEDAFAVASE
                     RADAIEGKAFDKLRNKAIERVEKANTFLEEKIGFLNTTIKTKIDETQSLIHSFNDNWR
                     NEANEYLTTMRLELKQMQGEYEETAEELAESLTEYRDESAIFFQKISTELFDGEKALE
                     SMRAEMQSVSDQMQSEIALLKTQLYDSLIGLKNEIEAESTAYNTEIKETFQSNIDEIE
                     NVFTNNLESSKNSFNDRFSSFQTEIEAKFLRFTGEYQSLLDTFSDDFSFKSESLEKGI
                     ADIDVIKQSLENSLKEISAKVLGDFSDFTDEQASSLQKHEENFANQMSLISTTIRGLE
                     NELQELKQRAYDNVSEKLSGFEDSFLEDLTKQSQRLNDSLENWQNAFDAKLENLALEY
                     SDERKVLENKYTEELKEKIQAIQERFTNYSNTLENSFDEKNKKYQADFLGLDERLVLF
                     IGEYKEAFEKTRIEADAYVKNEITHFIGSIDEQIKRYEKDVAQSLEKMTDDISDSSQQ
                     AAALLDSIRTDFSEWKERMNSQFAEVKTIFDDKYASFSEDAQNVLVDLERMLKDAVST
                     SSESALEQFRELEEKIEAISNETESVIQHYNKHSNDIVDDFKAMYKEMLEETERKIKT
                     QKAEVEEQLRQEKKIIQDIKLTNETSQEKMVLKMQKDSDELNTSLDTIDRKIKQFIAQ
                     TQIFEKADVLKLDLEEKISELKNETAQLVQYEKMIQSLEKQVEKLKKSEEEVNQRVLK
                     FNQEKKRIDDMEKNFNELV"
     assembly_gap    21389..21488
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21489..21830
                     /locus_tag="PKW26_00820"
     CDS             <21489..21830
                     /locus_tag="PKW26_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92141.1"
                     /translation="KIKDVLDQADSLDEILSTTEERFNAMQDSKHWLASTESRLNEIN
                     KETKSYLKLLGEVKKSPGTRDSRSSQGLVLETRENVIKLARLGWTPAKIAERLNISQG
                     EVELILETQGE"
     gene            complement(21863..22345)
                     /gene="ispF"
                     /locus_tag="PKW26_00825"
     CDS             complement(21863..22345)
                     /gene="ispF"
                     /locus_tag="PKW26_00825"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOQ92142.1"
                     /translation="MIRIGFGWDFHQLIKKRPLRIGGVHIPFHKGEKAHSDGDVLLHA
                     ITDALLGAAGIADIGELFPPEDNTWENASSAYLLQEAWKKVEEKSWRLVNIDAVLILE
                     KPKLLVHRETIRQSIASILGCKASQIFVKAKTHEKLGPVGRGKGIEAHVVCLLEKSET
                     "
     gene            complement(22342..23088)
                     /locus_tag="PKW26_00830"
     CDS             complement(22342..23088)
                     /locus_tag="PKW26_00830"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HOQ92143.1"
                     /translation="MNISLVLTAAGNSTRMKTSIKKEFMPIEQDETVLAASALAFLRY
                     FQNHTEHRLQSCVVTLPKKQIYQGMTSFFNEKTLDACIALKLSPEFIIGGKTRQESVY
                     KGLLKLSQYATKPDIVLIHDAARPFVDEKIIHDIITKTITHGAAVPVIASVDTQKKLD
                     TEKQCIAEHLERETIVSVQTPQGFSYKTIFEAHKKAEQSNKDYTDDSEIFSDYTNKAV
                     FVVNGNNKNIKITYQSDIPENVEKKGFGTA"
     gene            complement(23134..23757)
                     /locus_tag="PKW26_00835"
     CDS             complement(23134..23757)
                     /locus_tag="PKW26_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOQ92144.1"
                     /translation="MSKLKTAYALNQKIVYPSQGVGKIVDIKEKAFKEQMLLYYVIYL
                     EASDMTVMIPVERCDELGIRSIVDAKKAQETLDSLGDDFEPITNDWKLRYQMNLDLLK
                     KGSVDDIATIVRCLYHRSKIKELPILERKLYDSAKKLLEDEISFALEKTAKEVESLIH
                     TKLEPLGMQQAKPLSASQKDDDDDDEDEDVFDVNDDEDVDDDDDDED"
     gene            complement(23781..25358)
                     /locus_tag="PKW26_00840"
     CDS             complement(23781..25358)
                     /locus_tag="PKW26_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOQ92145.1"
                     /translation="MHFKAQKYLSRGFIFLLFISFFSTQLFAQKTENEISKIQEELLI
                     IQSPGHLNLNPQLSSYSSEAQILSGLYEGLFSYDPLSLEPIPALASSYKISRDKKTWT
                     FNIRKNARFANGDPISAQSFYDSWLKLIDPNTNAPFASLLDCIEGVQAYRLGKGDASK
                     VGIQVKNDGSLVIKLVHPTEHLAKILCHHAFSVIHENKTIQSGPFVISQLNKAELRLT
                     KNTFYWDADNVALPSIRFLFSEDQVENTYLFNIGKVHWLADNFDASKLIENDSLVFGT
                     QFGTEYYFFRMQNTSVWNSQKARQALLLALPWEKLREAFYVPATRLLSRLYNYPEIYG
                     VDESDIEEAQAILDSLGLNEEEKRIEISIPDTDYHADQVALFAESWKKIGIRTDIIKI
                     PSYDYYDAVPQSNSDMFTYTWIGDFADPMAFLELFKKGSSLNESRWSSPVYENLLQEA
                     TGIHDTKKRYEKLAEAEQELLDSFIVIPLSHPISVNIVDKAILGGWYENALDLHPLKH
                     LYFKERGPIKNLLIAIN"
     gene            complement(25348..26766)
                     /locus_tag="PKW26_00845"
     CDS             complement(25348..26766)
                     /locus_tag="PKW26_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92146.1"
                     /translation="MDYPETFSKELFSAFDAKLAWYDGEELPRLLSEYRKLHAYVENL
                     INTLLNKGSIHEDPYKHDKKISDIPQIDDGFYNENERNMVIGMRLSDLESALTFISNY
                     LTFSVASLNLERIKKLTALNAAFQWNSVSTNSTKPNARGLAEILATVRQGSDSLAISV
                     VNDSISNAGKSTAIINGILKNLVDFHKEMYKIEIRKILFSHPSFVNANPALNTQAYMA
                     QIKKVYTSVMGKQPFYTELIEEILVENFGPNAEKAQRDILEKLRVEKSETVVKEKTID
                     TKEILMDSVRILTGLVPQLTQIISKLEDNKKLLESEDSSFFERLSSFIRKVFNVKPRK
                     IHYRLTITNPITREQKTENIEIEQFLGNLHKRVRFYTSFSMKKTPGYKKIELLSNDKI
                     VEFIVTQLAENQTMLDVLLALEDYYKANISTIQQNKIKGIKMEIAALKNTLIKTNQRK
                     AEYVTLIEEQEQMKKLGITNAF"
     gene            26944..28695
                     /gene="thrS"
                     /locus_tag="PKW26_00850"
     CDS             26944..28695
                     /gene="thrS"
                     /locus_tag="PKW26_00850"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOQ92147.1"
                     /translation="MIHEKNEALETIRHSAAHVMADAVLKLFPGSKYAIGPAIENGFY
                     YDFDLSRPIKEEDLGEIEKEMRRIVSGNHDFSNKVLSREEALDFFKDQPYKTELINDL
                     AEDEQITVYTHADFSDLCRGPHVANTKEINGQAFKLMKTAGAYWRGDVTKPMLTRIYG
                     TAWQKPSELKEYLAFLEEAEKRDHRKIGKELDLFHIDDDNPGQIFWHPNGWTIYKTLK
                     NYVSDKIEKDGYVEVNTPFVMPQSLWLRSGHWEKYQENMFVTESEKRLFALKPMNCPG
                     HVEIFKTKTRSYKDLPLRMAEFGSCTRNEASGALHGIMRVRGFVQDDGHIFCTEEQIS
                     SEVVKFCILLKDMYKDFGFADDAIIVKFSTRPEKRVGSDESWDKSEAALEEACKAAGL
                     AYEVQEGEGAFYGPKLEFTLVDALGRHWQCGTIQVDHQLPSADRLHADYIGDDNAKHQ
                     VVMLHRAVLGSLERFIGILLENFAGALPAWLQPQQAVVIPVAPTVEGYAQEIQELVRA
                     AGIRSKINLGNDRMNAKIRAAQLEKIPYMLVVGEKEMAEGTVAVRFRDGRGQEVMTKE
                     AFISYAKDKIDNFFVGI"
     gene            28865..29425
                     /locus_tag="PKW26_00855"
     CDS             28865..29425
                     /locus_tag="PKW26_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92148.1"
                     /translation="MKKVLRTFFVIFAVLFVASCASNKGSRDVGIEGEKRPDWVKKMG
                     MSNDVTHYEVGYGKMSNYATSLKKAEADARNKIAFWIQTDVNTVLKTYTQDSGMGDNR
                     ELIEFMEEVSKQTANFSLSGAQIEDAWEDADGGVYVLMSFPVDKAGATLDNQLKTYQR
                     SESAAFAEFKAQEAFKQMQYEQSQGF"
     gene            complement(29511..30161)
                     /locus_tag="PKW26_00860"
     CDS             complement(29511..30161)
                     /locus_tag="PKW26_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92149.1"
                     /translation="MKKIYAFAFFCCFSLSLFSNAADRQGKLFLAAGPTLQINTDGLE
                     TAPSPISFSLGLGYSFSLNKDLAINPELGFWSLYYLYLNDRASPAAVEKRDVQALHFL
                     LDIPLLYKISTENNVFSFGLGIALLSRVAFATAVLPENEEYKVGKINDFFWSDFRYLY
                     PEATITWDWVLENGSSFGLSFKAFYPVANLMTELSSPLQNSLFSLKVRCFFPPQSK"
     gene            complement(30193..31419)
                     /locus_tag="PKW26_00865"
     CDS             complement(30193..31419)
                     /locus_tag="PKW26_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92150.1"
                     /translation="MCNRLYKFSKHFLPRGLTLFLNLFISSLLCFFIFSCKQNQIGRE
                     LETSDAILRASQIKTQKTHQWFYFSGNSFYETDHPSAVPRLIKKPWTETERISSSLSI
                     RNDKRDYDLFFTVNKVGILIAKPGHPKQAILASDQQLFSQLTAGALVCIDNFPVFHTY
                     KNSFFSRLTEKKEKLPFLVQFHPGTSIFLPLLYTSDLGISDATEVTDLQYAKGFWIAA
                     LKTESSEETQFDYLNFFSYEPITRQSAAKRSLHLEKKQLSTAEYREFLSPLPFSTAPK
                     RLQDLYKKLPPDYPFYCMVSETNAGSSLSYLNGSISDEKLIASNALIADSFSLAVFAD
                     GTSFFVGALPNKNILNQGEPLAFRLPRLPEHFSYGDFGVSGSTLYIAWEESFFYETAR
                     SGFLTVDLEELLYYAQ"
     gene            complement(31448..>31816)
                     /locus_tag="PKW26_00870"
     CDS             complement(31448..>31816)
                     /locus_tag="PKW26_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92151.1"
                     /translation="KNYEAVQIAIPPEHCTALLLSCDLEKSENFEKALAEKPWGFIAP
                     FDQEASEASTVASLVFFKLIQGASDTKAAIAYASYFNWEKLVESLEKQSDPFLLDIDL
                     MATAIAEGSFTSKSIKKLLP"
     assembly_gap    31817..31916
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<31917..32169)
                     /locus_tag="PKW26_00875"
     CDS             complement(<31917..32169)
                     /locus_tag="PKW26_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92152.1"
                     /translation="MNKTFLSLSLVFLFFVACKVDFLPSAPGENCIHFEPLRYISYPK
                     DLVWTLSYFDSEGKLIQLDIATKNYEAVQIAIPPEHCTAL"
     gene            32572..34410
                     /locus_tag="PKW26_00880"
     CDS             32572..34410
                     /locus_tag="PKW26_00880"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOQ92153.1"
                     /translation="METISFEDLGLDAATLAAVEQKGFESPSPIQVLAIPRLLNGEAN
                     VIAKARTGTGKTAAFGLPLVQSIRSDLGKVQALILAPTRELALQVAKEIESLSTGKYP
                     RLTTVYGGQSMGEQLRSLKRGVEIVVGTPGRVKDHINRGSLKLEDISYFILDEADEML
                     DMGFIEDIEFIFSKANQDSRVLLFSATMPKEILKIASTFMGDYEIVEEEEQEEAPLLT
                     EQKYWVVREGDKTEALVRLIDISPEFYGLVFTQTKLDADMLSRQLDERGYEAAALHGD
                     IPQGQREKILSRFRSKKTKILVATDVAARGIDIEGLTHVVNYALPFDGPTYVHRIGRT
                     GRAGAKGLAFTMVKPEERRRLEYLKQVAKKATQGELKEEVIPSIEQVVDVKKTRLFGD
                     VKSSLNLTQGPSQTFLTMAEDLVGTEDSLSVLASVLEKNYGNLIDPKRYGAIRTPQKP
                     SLGTRSGDREASNQVRIYVQLGRKDGFNPREIASYFSQLLGIPQRMVDRIDTAHAFTL
                     LSLPKKEALEIIERTKWDKSLPHMHLDTKENARVDRDNFGNRGSQGRNGRRGNSFAGE
                     SARGSRRASFSSAKAGFKEGGGRKNAHPRDYSSRGKTSSAALYKKA"
     gene            34417..35856
                     /locus_tag="PKW26_00885"
     CDS             34417..35856
                     /locus_tag="PKW26_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOQ92154.1"
                     /translation="MSLNWKEIELVLEELAISGSFVQNIVQPSFDALALYLYKEGRAQ
                     TLFISLASGSCRLHETRRKIPKNPKPLRFMELLKKRILGAKIVDAKQLGQERIVQLEL
                     EKNDERLFLFIRLWSGAANILLTDSDFGIIDAFYRRPKKGEITGQIFSLPLPREGEKG
                     RDYDLRSFESLADFGLDISSLSLSEKIEKWYEFCGAQLSVESLVEKVEKHFAQKIAAI
                     EKLLQSIEAKKEAFSNREVLKIRGDLILSNAHLIEKEQKILRCLDYTTNTEIEILLDS
                     TKNAQENANELYRQYKKAETGFEALAIEEKKLEKEKQALFEEKEAILLEGNPLRLEQV
                     LRKIETPKQVAEVERPGLWFDFQDWTIIVGRNARENDELLRHYARGLDTWLHTRDVQG
                     GFVFIKNKPGKTIPLPVLIAAGNLAVYFSKARSEARADLYYTQVKHLKRARGGKKSEK
                     SSLTGKVLPSHEKNLFIQLDTRLLQELGL"
     gene            36091..37251
                     /locus_tag="PKW26_00890"
     CDS             36091..37251
                     /locus_tag="PKW26_00890"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOQ92155.1"
                     /translation="MVEKSFENISRKVALASCAEYNEKSVLEKMRQLVIDAPPPSVEG
                     KTVLLKPNILSPKKPEFAICTHPVVVASAVKVFLELGAKKVFVGESPAVAPSLVAAKA
                     SGMLEAIEKAGAEWVDFFEMQTLHFEGGKLIKSIDFAKPFFEADLVVSLSKLKSHQLM
                     AYTGAMKNLFGLVVGLNKAQMHYRFSDKNDFSKYLTDLVVASGSRYAIMDAIVGMDGP
                     GGPGNGDPVPLGFLAASQNLLALDWTCASMVGYKPQDILNLSDAMSRGLWLKDASEIE
                     LVGETVESIGPLQFRTVTETSGLTSLQKMLPDWVNGLAKKIFVKTPAFQHSTCIRCGR
                     CIEICPPQTLSFEDDEKSKWQKKVVIDKTDCIHCFCCHEICPVDAIQLKRFQ"
     gene            37326..38219
                     /gene="metF"
                     /locus_tag="PKW26_00895"
     CDS             37326..38219
                     /gene="metF"
                     /locus_tag="PKW26_00895"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOQ92156.1"
                     /translation="MKISKIIDKKKLLISFEIFPPKQEANFDSVMQTALDLGGLNPDF
                     ISVTYGAGGTTRSYTAELASSLQEKTQIPSLAHLTCVGSGFSDIQSIIRELKEKKIEN
                     VLALRGDYPQGADASDLPGDCAYASDLVRILKKEADFCIGGACYPEGHPESENRLEDL
                     QHLQYKVDAGLDFLTTQMFFDNEVLYSFLYRMQSKGMKLPVFAGIMPVTNAKQIKRMI
                     TLSNAHMPQKLLAFVDRFQDNPEAMMQAGIAYATEQIIDLIANGIRGVHIYSMNKPDI
                     TAAIMHNISHIVEAVNAEAHV"
     gene            38238..40754
                     /locus_tag="PKW26_00900"
     CDS             38238..40754
                     /locus_tag="PKW26_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOQ92157.1"
                     /translation="MKQDFRLFLEKSLRESRPIIFDGAMGTMIQDSGFTDYLLPEELN
                     SKKPDLIRGIHEAYLASGANVLTANSFGSNGIKLKDASFSAAEATTAAIENLRSLIDA
                     RDRSRFKQEVFAALDIGPTGQLLAPMGRLSFDEAYEAFKESALAAEKAGADLVIIETF
                     SDLYEAKAAILAVKENTSLPIVALMTFQSNFRTLTGSDVETTVCYLESLGVDALGFNC
                     GGSLEEAKTLAKLFLEHANLPLVSLPNAGLPVIEKGQTIFKVGANEFSKVQKEIATMG
                     FSLLGGCCGTKPEHISALVQDLQHFSIPKNNRPSQRRRSSLCSAEKTVYIDNSFGSTG
                     PVIIGERINPTGKKKLKEALLSNDMSYILDEAENQIQAGSHILDVNVGLPGLDEKAKM
                     QEVVAALQKNYATPLQLDSSEPEVLEYAMRYYNGKPLVNSVNGKQKNMDDVFPLVKKY
                     GALVVALCLDEDGIPSTVEGRLSIAKKIYAEAQKYGIRAEDILFDSLTLTLSSQQEEA
                     LVTLDAIKAVKKEIPGAKTVLGVSNISFGLPRRDIINAAFFSMALYAGLDACIINPLS
                     EEMMASFNAYRTIAAFDDKALHFIETYSGTQKISSSIANKAATKEDLSVSKETEQKSL
                     FAKDDLQTIIIKGQVDKAESCVEKLLEAGKTSLDIIDGCIVPALDIVGKEYESGKKFL
                     PQLLLSAETVSKAFGLIKAELAKTGIQDEAKGTILIATVEGDIHDIGKNIVKAMLENY
                     GYEVLDLGKDVSAETICDLAVEKNIRLIGLSALMTTTVLNMEKTIKLLREKEKEMAEK
                     FTIMVGGAVLTEDYAKTIGADFYAKDALASVQIAKKFFGK"
     gene            40758..41654
                     /locus_tag="PKW26_00905"
     CDS             40758..41654
                     /locus_tag="PKW26_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92158.1"
                     /translation="MKCEEDEFPSLKTFSIARAYFYVILNPKSLWALLCYLRTVLCDF
                     FLLQFSVKFGFKNLEIINVDHGLDDSVPFDPTKVHVYLDFVNFWIRPMSFMLKRFGVK
                     KAIPYCARYLNAIERCYSEASRMYRFRMSTTNRPPADGRKGFRMIHLLDPHYLCVPSL
                     HVAIVNLAYNFFRDAFTDLGMEKEELTFYTSELYAGAIEITETVLYVKQHSVNCIPAA
                     LYMCLFILQDQFSIPDSVRFIESLFLDSTDIRAEDVEAIQDHILFLFEQLLLEGSIED
                     DWTAPVKRWILNYASPCSEKYA"
     gene            complement(41668..42999)
                     /locus_tag="PKW26_00910"
     CDS             complement(41668..42999)
                     /locus_tag="PKW26_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOQ92159.1"
                     /translation="MKKILVASTNEGVLKTVRAACKKYANYFDAVFLSTTDETLSYVS
                     YELPEIKVLDFTSENNIDANNILNEIHADPWLHNGGIIAIVANNAEAQKLEEKKDSNI
                     LVIQTVKSFHENFDRLLRILWSNQHFLFNRGIQERIGGRETGSFVCGSDPLDIRMYTN
                     FLVSYLYNTNRISSDDRYKLQTTLMELLTNALEHGNLQISYEEKTKWLNNGGNILELI
                     YRKNKLPEYINKKIYISYIIGKNKSRFRIRDDGDGFDWKARLEKEFTDHTHGRGMKIS
                     QSLVDSLTYNEKGNEVTFDILNLQDEANTVPGIMKPFESEVYKDKEVVCYENDNTNDL
                     YFIVSGRYAVYTNRKLISVLTPNDMFIGEMSFLLNDRRSATILSIGEGRLIKIPKVSF
                     LQIIRNNPHYGIFLSKLLAQRLLKQNRKTLMLAEEIVELKKLADIPEDFDE"
     gene            complement(43093..43725)
                     /locus_tag="PKW26_00915"
     CDS             complement(43093..43725)
                     /locus_tag="PKW26_00915"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOQ92160.1"
                     /translation="MNFIFLGPPGAGKGTLAAKVAEMHNIPHISTGVIFRAAIKSKTA
                     LGLRVQSIIDAGELVSDEITIELVKERLTQEDAKKGFILDGFPRTIPQAEALAKIILI
                     DHVVNFNIEDADVIARLSGRRVCKSCGQNFHKDFMPPKQEGICDKCGSELFIRDDDKE
                     DAITHRLEVYRDQTEPLIAYYKKEKLITSIDARPNTETILKDFENTFYGK"
     gene            complement(43857..45182)
                     /locus_tag="PKW26_00920"
     CDS             complement(43857..45182)
                     /locus_tag="PKW26_00920"
                     /inference="COORDINATES: protein motif:HMM:NF009881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ92161.1"
                     /translation="MDLFTQRAEKNEGPLASRMRPRNLDEFFGQEHIIGEGCLLRRAI
                     TVDQLSSVIFYGPPGTGKTSLAHIIAKQTKRNYLSLNAVLSGVQGIRDAIENAEKTQK
                     LYGKKTILFVDEVHRWNKSQQDALLPWVENGTISLIGATTENPFFEVNKALLSRSRVF
                     ELKSLSEENLKDAAHACLKDKERGYGRWKVVFEKGALEHLIKTANGDLRSLFNALELA
                     IETTPAIWAPYEEEPLPPLESEIFISKKTAEQSIQKKAILYDKDGDYHYDIISAFIKS
                     LRGSDPDAALYWLARMVRAGEDPHFIFRRMLILASEDVGMADPYALLVVQAARQAFDQ
                     IGFPEGQYQLAHAALYLATCPKSNSIEAYFEALAEVEKEDQEVPNHLKDASRDGERLG
                     HGKGYLYPHHFDGHWVKQDYLPAKLLGKTFYTPGTEGYEAKLDIKKNKK"
     gene            complement(45193..>45688)
                     /locus_tag="PKW26_00925"
     CDS             complement(45193..>45688)
                     /locus_tag="PKW26_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92162.1"
                     /translation="AYVTTSGDDGKLYLLSHTTTTEVIDGDKKSVAAVHDGTETVFSN
                     TVLIASGTKGVYTMDGNKIKYGTETSSIEVSGAKSMALYNDSLLIGTTSGIVLVNLDN
                     DGKPTSTGTMFGENIESSIAMLEIKTLYSFGDAIYAFAVDKTTSKKTNTWGYYKDRPN
                     WNVE"
BASE COUNT        13795 a         7956 c         9721 g        13916 t
ORIGIN      
        1 tttttctaac acggatgtta gaaaaaacgt aagatggttt tcttccccta agaaaaacct
       61 tattcgcctc gtagttggcg tttttgggag gcgtggacgt ttttctgggc aaagcgatgt
      121 gcttcgtcgc ggattctttg caggaggagg cgggcgggag agtttgaggg gaggcggagt
      181 ggctcgtttt tatcttgtag ataaatctct tcattttttt cagcaagggc gatgagggga
      241 atgtctattt caagggcttt gagtatacct tctactgcat ggagttgtcc ttttccgccg
      301 tctatgagaa tgaggtcggg gaaggggagg ttttcattta atagccgaga atagcgtcgg
      361 gcgctcgctt cttttaatga gtgataatca tctattttcc ctttttctaa actttttatt
      421 gtaaaatagc gatattcttt tttatggctt ttccctctgt aaaactgtac catgctcgca
      481 acggtaaaat gaccatcgag gtgggaaata tcaaagcctt ctattactag agggagtttt
      541 tctagcttga gtatttcttt taagtcttcc atgccagcaa aatcgcctcg ttcgcttaag
      601 cgttttcgca agtcctcttt ggcattttgt tctgcaaggt ccaatatcgc acgatgtcgg
      661 ctttgggcat cgtagtgggg tttatcgaat gcaactgcaa gttcgccttc ttcgcttttt
      721 tcttcgtcgg tcaggttttt tgcaacttca cctgccttgg ggacgagtga aagcacttgt
      781 atagctgttt tttttgtttt tgttaaatat tcactgtaga gcttcgtatc tattgcaatg
      841 gggaggtaga tgtattgggg ggcgtctttt tcttcgtaat agcttcgcaa aaattcctct
      901 gcaaggtctt tcaagcttcc tgcatcgtgg tggttgtgga tgctttttac ccgcagggag
      961 tctttttcga ccatctttcc atcgcgcatt ttgagaatcg atgcttggac aaagctttgg
     1021 tcaaaggcaa agtgaatata gtctcggtct tcaaaaatag aatcggttac gaggtttgtt
     1081 cgtaataaat ggctcagtga atcgtggaga tcgcggtatt gggctgcttt ttcaaaattg
     1141 agttcttttg acgcttcttt cattaaaaca gagatttttt caagggtttc gctcggtttt
     1201 ccttcaagaa aggcggtgat ttcatcgata tataatctat aggcttcttt tgaaatataa
     1261 tcgcaacagg gagcggaaca tctgcctatg tgataataga ggcagggact ttttcttttt
     1321 cgcagtgttt tgcaacgtcg tagggggtag tttttataaa tgagctctaa aaacgtttgt
     1381 aacgcggtac cattcggata gggaccaaaa taaagggagc cgtcttttac gatacggcgt
     1441 gttttgaaaa tgcgtggaaa atcttcgttt gttatgcgga taagggggta ggatttatca
     1501 tctttaaggg aaatattata ctgtgggcta tattgcttta tgagggtgtt ttctaggatg
     1561 agtgcttcat attcgtttga acagataatg gtttcgattt ttgcagcgcg agagagaagt
     1621 gtacgggttt ttatatcttt tttccctgaa aaataagatt tcaagcggtt ttttaaattt
     1681 tttgccttgc cgacgtagag tactttttct tcggcatttt tccaaaggta tactccgctt
     1741 aggggagggg ctttgagggc tgtttcatgc aagctcttta tagtattcat ggtggtatat
     1801 gataattgca aataaaaaaa atatagtgat ttttctcatt gggttttgtt ttgccttttt
     1861 gcttcccgtt ttttctgaag aggagtcgat acagatcggt ggaaaaaatg gttggcaaaa
     1921 tctcctcgtt tctaaaaaca ttgtatcaac aaaggggctt tttggctatg aggcaaaggc
     1981 tttggcaaaa aaagaaaggg cgcatacaga aagtcttgac ttactgctca gctttgatgg
     2041 ttcggatata aaagatacgc ttgatagata tagggttgtt aatgaggatt cagtcaaaac
     2101 gtatagaaag tcaagtgatg cgaaaactgg tacagcggca cttttaagtc ttgaaaaaaa
     2161 acctcttgtc ttatatggaa ggggagatgc tttttttgct tcatcagggt ataagggttc
     2221 tttttcgatt agtttttgga taaaaccttt tttactgcaa agtcaagagc gtttgttttc
     2281 ttggaactct tctcgaaata ctttaaacac agtcttggat caatctattc aagcattttt
     2341 taataagggt agattgcaat ggcaactttc aaatattttt actacttctg aagcgattga
     2401 aacagatttt cttctgaaag gtagttctat gcttatccca aatagctgga gtcatcatag
     2461 ttttgtgtat agcgaggcag atgaaactat cgagtactat gtaaatggtt ttctagagga
     2521 tgttctttcg gttaagactt tgaaacccta tttggggaaa caggctgata ttagactttg
     2581 tgaaaactat acaggcttac tcgatgcctt tgttatcgat aatgatgatg cggttttaaa
     2641 tggggatggg gcggtataca gcaaggaaag gggtcgtttt gaaacgcagc ctattgattt
     2701 gggaacttat gggactgtag tacgtaaact ggaagcagag gtggatattc cctcgcaaac
     2761 agcggttgcc ttttttcttc ggtcaagtga taatttttat gagtggacgg aaacctatcc
     2821 tgcttggcag cctgttgctc caggaaagca attagatggt tttatcggac gttatgtgca
     2881 agttgccgcc gaattgtata cagatggggc aggaaaaaaa acgcccgcta ttacagatat
     2941 ttcgcttttc tatcaaaaaa ctgaagcacc gcttgcaccc tatatgctaa aggctgaggg
     3001 gggggatgga tatattgatt tatcctgggc aaaaacgctt gatgaaaata cagcaggcta
     3061 tttaatttac tatggagaaa gctctggaga gtatttaggg acctttgctt tggaaggaaa
     3121 ctctcccata caaacagagt tgcaagaaaa atttcgaata aggggtttgg aaaatggaaa
     3181 aatatattat tttgcaattc gtgcgtattc ggcagttgat gagagaataa ttggtccatt
     3241 ttcaaaagag atatgggcga gaccacgaga aaataaggga gctttttatg agtaacgaaa
     3301 gagattattt attacacagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     3421 tcatgggaaa caacgaagag ggagcaaaat gttttcaacg agttattgat gaaaacccca
     3481 atgatgtttt agcctataat cacttgggaa gtattcaagc tgctcgtggt gagcatgcaa
     3541 aagctttaaa tacctacgcg aaagctttgc agctggataa aaaccatccc atcttgcatt
     3601 ataatgctgc aaaatcctat attgcattgg gaaatctctc taaggcaaaa aactcctatg
     3661 aaaatgcact tcgcgcgcat cctggttgga ttgatgcgat ggatgcatat gcacgattat
     3721 tgatgaaaat gaatttactt cctgaagcag aagatatctt actacaagca caacgtttag
     3781 ataaagataa tcttgccata aaaaatgctt tgggtttagc ctattttaag agagaacgat
     3841 atactgaggc aaaccgtctt tttaatgatg tactacgtga aaataaaaaa gatgtgtcag
     3901 ctcttttggg taaaagtgct atattatcaa aaacacgtaa aatggatgcg gcttcgctta
     3961 ttttatctga cctagaaaaa aaatatccag ataatcgtga ggtacgtttt caatactcag
     4021 atcttctcgt gagaatgaac aaacttgtag aagcagtgaa gcgaataaat catctttatg
     4081 cagaaaatcc aaatgaccct gatttgctta atcttatttc gcaatatgca attcgaaaag
     4141 gcgaagaaga aagggtccaa gactttgtcg ataaaattgc tcaaaacaat ccggcgtaca
     4201 caaaacattt ttttgatatt gcagacgttt attttgaaat gcaaaaactt gataaggcag
     4261 aatattttat tcgtagctat ttagaaaaac aaaaaaaaga ttctaaagcc tatgttttaa
     4321 tgggaaaaat atatgaagta ttaaaaaact atagaaaagc tttggaacac tacaaaaaag
     4381 gagttgactt aaatccttat agtaaagaag cagaaacatt gttaaaacga gtagagccgt
     4441 ttgtatataa ttccgaagaa aaaaaagaag aagtgcttga tgaaaataaa ccagcatccg
     4501 aatatttagg aagtttcaac atagaccttg atgaagaaga tgattttcta tttgctgcag
     4561 gagagggtct tggcgatgtc ttggatgaag gagatcaggc tttggcagat acggaggcgg
     4621 tagacaatga ggagacaatt ataactgatg tagaaccaac aagcgaagat ttttttagcg
     4681 aagtgccatt agctcttgat gaagaagcat tgtttgaaga accgcttgat tttgatttcc
     4741 cgtttgaaga tgaagactta gttgacgata atgacgatgt tattactatt gaagatctaa
     4801 ttgatacaga tgcgcccttt gatgaaaatc ctgctttgtt tgatgatcca ctttttgatg
     4861 attttggttc atccccgaca caatatcttc cggatgaaga ggcagaagtt atggacatct
     4921 tagatacgcc tcaagggttt acagaagata gtgaaaatga caaagctcta ccaaaacagg
     4981 aagagttctt ctctcctcca ccattagcag caaggccgca agcctatccg ccatatgaag
     5041 agaaaatgga aattccacaa cagcaaacct ctcctatgca tgaaaaggaa aaagaaattt
     5101 gggaagatga tcctctttta gaagagagag atgaagaggt ttttttatca gacgaaagta
     5161 tgcacgcaaa agaggaagct gcgcgaaagc ctgaagaaaa aagagatagg cgaaagagcg
     5221 atacagaact tgaaaaactg aaggaagact atgattctct tggaaatcgc tttgataaaa
     5281 tgcgagatcg atatgataag ttgagggatt ctcaagatga actacgtaat cgttttagcc
     5341 aactcgagga taattatgac gatttagatg ataaatatgc ctatctttca caagatgcag
     5401 atttaataga agatataaaa gaagataaag gactttccga atccgatgaa ataaaaggaa
     5461 gagaagaagc attttttgta gatactgagc ctggcttaga tgaggattta cctgttgaga
     5521 ccaagcttcc ggataagaat ataaatgaag atgagatttt ttctgtcaat acgggaaaag
     5581 aggaagaaga aggcaatagt gaacaagctg ggataccatc gctttttgaa aatcttgaag
     5641 atttatgtac ctatttacct gagaagaaaa agcttgattt cctttcaagc atagagcgtc
     5701 ttaagatgca ttatttaatg caaaaaatgc tcggaggaaa ggggctcttg cttaaatcag
     5761 aagatgtacg caacgaagct ggcatagcgg tagaccaact tgatgacctt gatgctgact
     5821 ctgtgctcat gttgtttttt catttgcaaa atctcacaac agagttacga gacaagcaac
     5881 tgggcgcact tctctatgaa aaactttcag agattaaaga agcttgtagc gcaatgtggt
     5941 agcgcatttt atattctttt acggaaggcg aatcacgcgt gatcgagtta agggttcggg
     6001 tcctgtctca gtgattaaaa tagtgttttc taagcgacaa ccaccgactt taggatcata
     6061 gagccctggc tctaggcttg ctatcatacc tactttgaaa acagcactcg aacttgtttt
     6121 tgttcgtatc cagggagctt cgtgaatttg tagtcccatg ccgtgtccaa gtgaatgtgg
     6181 cattttacgt tttgcctttg caaaaaactc atcgactttt acggcagctg tttttactgg
     6241 ggtaccttct gcataaaaag gaagcgctaa gtcgtaggct ttttcaacaa gagagatttg
     6301 tttttcttgt tctgcgctta agcttccttt tgcaatggtg agtgtaacat cgcttgcata
     6361 gccatcgtag tttacgccaa agtccaatat agaaagtcca gaagcaggaa agttgccgga
     6421 tgtgtagttg ggaaagcagt gaatagcaaa acttcgagat gggtttgcaa cgaggcttgg
     6481 aaagctcgtt ccctcgcagc catgttctcg gcagcttctt tctataaaga gtgcgacgtc
     6541 catttctgtt ttgaaagcat tttttttcaa agcctcggca atcgcatcta tgatagtgtt
     6601 tgtgatttct gctgctttgc gggctttttt tatttcagct gcatctttaa cctgtctttg
     6661 gtttgtaaca aattgatgag caaagttttc attgcatagc acatcgtaat ctgcaagcgc
     6721 atcgacatag cgtaaaaact ctggataact tgttgaagag ggaatttcca cccgtgtttt
     6781 tcctggcact tttaaggttt tcaaaagcgc aagacttgcc tttatgggtt tgagttcgta
     6841 ttttgtatac ggaataatga ggtctacatg ggctttttct tttgcaagat tttcatccca
     6901 agggcagagt atggagctgc ctttttttgt gataattaat aatgcatctt ccgcatggtt
     6961 tgtaaaatag cgaatttcag ggcttcgttt ttcttcgtta tcgacaaaaa taacagcgga
     7021 tatatcgttt agactcatcc aatctgcaag tttctgtctt cgtgccttat atatttcgtt
     7081 catacatttc ctttatcttc cgttttggaa aattttttgt tttatttttt ttgcaatact
     7141 aagtaataaa ctatcttttg taagcagcaa gagcaagatg cctagactaa aaaatatgag
     7201 cgtagcaatg gccatgggga ctgcttgcgc gataagttta ttgtacccac taaagatact
     7261 caataatcga ttttttatca aatagacggg tgcgatagca atgagcgata aaagagcgag
     7321 ttttattgtg tagcctataa tctgttttgt taaaaaaact gcttgcatat gtgagagcct
     7381 atgtaagaag aataaaagcg caatgctgtt tataaaactt gctatagaaa gtgcgagcgc
     7441 aatgccgttt cctcccatgc tccgagagag gatgagtgca agaataatat tgcacgcaaa
     7501 gccgataatt cctgcatagg tgggcagttt tgcatttgac tgtgcataaa aagcagggct
     7561 aagaatgcgg tttgctgcga tacagaataa gccgattatg tgaaagaaaa aaacgccaga
     7621 ggtgagtgcg acggactcat cagaaaattg atttgttttg taaattaaga taatcaagtt
     7681 ttcgcgaaat aggatggaaa aaaaagttat gggaatggtt atgagggcaa tgagttttag
     7741 tgcgctgagg agcatttggt tatagtcctg ccatttttct cgttttgcta agcttgaaag
     7801 atctggcaag atgattgtgc cgacagttac tgcgaaaata cccaagataa gttcttgtaa
     7861 gcgaagtgaa tactggatgc tggataaaat accaattccc gtattttttg cgataataga
     7921 ggaaacaaaa tcatttagct gataggctgc catacctatt atggttggtg cgatgaggcg
     7981 taaaactcgt tttgttccag ggtttgatag cgttttttta aggccagtga ggcttatttt
     8041 ccagccttgt ttcagaacaa agggcagttg aaaaagagct tgtactgttc ctccaagaat
     8101 cactccgata gccattgcgc gagcggggtt tcccgcataa tcgataagaa ggtaggagga
     8161 ggcgattacg atgatgttaa aaagaatggg agtaaaacca ctcggtgaaa aaatatgatg
     8221 tgcatttaag atgccttgaa aaaatgccgc gatggatata acaaataagt agggaaacat
     8281 tatgcgtgtt aaaagagctg tttcagaaat aatgtctgcg gagttatgct tggaaaaaag
     8341 cactgcgatt tgtggggcaa agataatgcc gagaataacg gtgattgttg ttgcaaggct
     8401 tacgaggctg agagttgcgg aaagaaaggt ttgaatttcg cttttttctt ttttatcatc
     8461 atcgagatag gagcgaaagg tggggataaa ggcaactgaa atagagtttt ctgcaaagag
     8521 ccgtctaaat aagtttggaa gcataaaggc aacaccgaag gcatcggaaa gttggctggt
     8581 tcccatgagg gcagccttgg ttatttcacg gataagacca agaatccttg aaataagggt
     8641 aaaaaaggaa agagaaagac cacttgcaag gatgtttttt ttctttgttt ttgcatcaac
     8701 catagcaact tagcataacg agcttgcaaa aaagaatcaa tgctgttaca attaaaggaa
     8761 atagagattc caaagaggta taggcatcta tgaaaaaagg tttgtttttt ctaaaagttg
     8821 gaaagacccg cattattcca ttatctgtaa aactcgttag tgtttttgta gtcttgttat
     8881 tattatcaaa ttttgcaact aattttatca atctccaaat gaatcaacgg caaattacta
     8941 ttttgtcaaa taaaatttta gtcagtcaac ttaaagatat ttataccgtg gtacaaaatc
     9001 aatataaggt gtatgaattt tcaaaagata tagatagttc tttgcaggct atagaaatgg
     9061 tgtctagaaa tgcactttca tataaaaact ctattgtaat gggagctcgt ccaggtgcat
     9121 cttctttttt ttctgtggcg tacgaggacc cctttatccg ttttccagat gagttactct
     9181 tgcttgagat gaaggattct ctttctcaag gtattgatga aggctctatt cgtttttctt
     9241 catcgaaagg aaattatttt ggagtctaca aatatagtcc agaatggaat gtttttatta
     9301 tacgcgcaga cctcattgca gatgtggaaa aaagtacgaa tcgaaatttt attattattt
     9361 ctgctattat tcttttgttt actattttat ttttatttgc tggcttggtt atgttctcaa
     9421 aaattcttgc gagtatttca actattacaa aaagcttgta taaaatgcaa caagagcaaa
     9481 aactctctct cttagattta agcaattctc caaacgatga tattagttat cttgcggctt
     9541 cttttaattc gctttcaacg actatcaata atcttttggg aatttttcaa aaatttgttt
     9601 caaaggacgt tgtcgaagct gcctataagg agcactcgat tcgccttgaa ggttcacaaa
     9661 aggaccttgc gattcttttt tctgacattc gtggttttac ctatatgaca gaaaccctcg
     9721 gaaacgatat tatcaattta ttgaatattc actatgacaa ggcgattgct gcgattcatg
     9781 aagaaaacgg tattattggt tcgattattg gagatgcgat tttatcggtt tacggggcga
     9841 tgggctcgta taaaaacaat tctcttgaag ccttacgttc cgcttggcaa ataacccgtg
     9901 ttaccgcaag tcttcgtgaa caaatgattg aacgacgtaa ggaaatagaa aaacagcgcc
     9961 cgcttacaga ggcagaagag cgtgttttcc aagctgttct cattgatgtt ggggtagggc
    10021 ttgacggtgg aaaggttttt tatggaaata tcggttcctt cgaacgcatg accaatacgg
    10081 ttattgggga taatgtaaac tcggcagcac gtttggaagg gcttacccgt ttatataagg
    10141 tgcctgttat agcttcagat tatataaaag agggggttga gtctgttact tcacagttta
    10201 cctttatcga gcttgatact gtgcaggtta agggaaaaac agaagggaaa aaaatctact
    10261 ttccactgga tagcttagtt acagatgctg aaacgctcga aaaatatgcg gtatttagtg
    10321 aagcactgag cttttattac aaaggcgatt ggacagaggc acgaaaatta tttagagcat
    10381 cgaagctcga acttgcaagg gtctttttag atcgcattac tattaaacaa gcacctaagg
    10441 attggagtgg catatgggaa atgacgacaa aatgataatt cataagccgg agttaaagct
    10501 ttttctcacg gacaaaatgt atcctgagac aaagacagag tccgagcact atgatttaga
    10561 gaatgaatac gaaaagacaa aacagaataa aaatcacaca gttttactga tgcttttagc
    10621 cttggttgct cttgttgttg gaatttccta cggcattaca gcgtttattg cgtatcagaa
    10681 taaaaaaata gatgttgata taaatgtttt tgctgacaca aatcttattg aactgtttga
    10741 tatggtgagt cgaacagaag acgcacttgc ctcagcacag tcagaaaaag cccgtataga
    10801 aatgctacgg caaacagaac tacaagagat agaggcaaaa aaagaaggtg aattatatgc
    10861 cttgcagagt atgcgtttaa caagagctgc acaaaatcaa caggaaaaag aaatacaagg
    10921 agtctatgag gcagccctat tagatattca agaaagctat gatagagatt tagctgtttt
    10981 ggaaaaatcg attgaagaat atcgaaaaca acttgaaacc ttagacagtt cgcgtgtaaa
    11041 acaagcacaa gaacagcaag ttgccctcga ctttcaaaga catatctacg aaatggagaa
    11101 agttcaactc caggaaaaat atgaacttat tattcaagat ttacgtgaaa aaattgaaga
    11161 gcaacaacgt ttggcttatg agcgtcaaat ggcaagtatc gatagcctaa gtagtgatta
    11221 tcaacgactc atagcgcttt tggaccccat tgttgaagat ccaagggttg atgaaataaa
    11281 agcagagata gcagagtttg ttgaaatgcg cgctttctat gcagatgact atagaatttc
    11341 tgctaaaaac gaagtatata atcatttatt ggaaaaaata gagcatgcct atacatctat
    11401 cgattattta gctgaaatag tgagcgcaat tccttatgaa aactctatac catcaatttt
    11461 agaaacaata gaatctattt attatgttat tgggattgag ttagcgtatt tttcatcgaa
    11521 cgaaattgca agtttacaaa aaagcttaga tacagtgcat gctgactatg cggcagaaat
    11581 tgcagccttg gagtctagcc ataaaaaaaa cttagaaaac caaaaacttt cttttcaaaa
    11641 tcagctggcg gatcaaaaag acacatatga aaagaaactc attgcgcagg caaatgaatt
    11701 tgataaaatt gtagaaaatt ctcgttccta tcttttaggt ttagagcgcg aactttctaa
    11761 aaacagaact tatatagaag cacttgaaaa agaaaataga gatttgaaag agaatcaaaa
    11821 acagatagaa actgcttctg aattcagtgt ttttattgac cttgaagaag atgacttaaa
    11881 agagtatgaa gatgatagag aagataaaaa agaaacaata caaactctta tagagtaaac
    11941 ctttgttata aaattacgat acaatactat tatattgtct agaatctcat aaaaggctat
    12001 actttctgta tagcgtctgg tatatattat ggaaataagg taaaaatgtc aaaaatagat
    12061 acagtagatt ttttaacggg tagtgaaccc ttacgggttg gtattgatgt tggttcaaca
    12121 acagtaaaga ttgttatttt aggtgaaaac gatacaatat tatttagtaa atatgaacgt
    12181 caccgtgctg atattcgcac aacgattata gcggttgtaa atgaggctct cgatgtcgct
    12241 gaaaaaacct atcctgaagg aaaggagcaa cttttaagtg taaaagtaac cggctctggc
    12301 ggtcttgctg tttctcattg gcttggtata gattttattc aagaagtggt cgcttcgaca
    12361 cgggcagtgc aaaaactcat cccgcaaact gatgttgtta ttgagcttgg tggtgaagat
    12421 gctaagataa cctattttag tggcactgtt gagcaaagaa tgaatggtac ctgtgctggt
    12481 ggcacgggtg cttttatcga tcagatggct gccttgcttg aaaccgatgc gggtggtttg
    12541 aatgaactcg cgcgtggggc gacgatgatt tatcccattg ctgctcgttg tggcgttttt
    12601 gccaaaacgg atgtgcaacc gctcataaac gaaggggcaa ggcgagaaga tatagccgca
    12661 agtatttttc aagcggtggt aagccaaact atttcggggc ttgcctgtgg aaaacctatt
    12721 cgtggaaatg ttgccttttt gggtgggccg ctttacttcc ttgaccaatt gcgtcatcgc
    12781 tttgttgaaa ccttaaagct tgaaggggag gcaattatcg ctcctgaggg ctctcagctc
    12841 tatgttgcag ctggtgctgc cttttctgca gaacgggatc tttcacagat tgtgcctgga
    12901 gaaaaatctc cttttgtgag tattgggaac ctgcgagaaa atttaacaaa gcttgtcggt
    12961 gcagaaatga cggaggtgca acgcctcgaa ccgcttttca aaaatgaagc agaacttgaa
    13021 gcttttcaca aacggcatgc acaagaaatt gcgatgacct atgagctgga taagtcccag
    13081 ggaccagttt ttctcggttt agatgctggt tcgacgacaa caaaggcggt gctcatcgac
    13141 gaaaaggggc gtattttatg gcgcttttac gatgtcaatg ccggaaatcc cgtcgaatta
    13201 gcggtgcgtg ttttaaaaga cttgtataaa aaacttccca aagatgtata cattgcacga
    13261 acggtttcaa cgggctatgg ggaagcgctt tttcaagccg cccttaaagc agatgccggt
    13321 gaagttgaaa cgatagcaca ttatcgtgcc gcagaatttt ttgtaccagg tgttgagttt
    13381 ttgctcgata tcggtgggca agatatgaag tgcttgcgta tgaaaaatgg cgctattact
    13441 tccatacagt tgaacgaagc ttgttcttca ggttgcggca gtttcttgga caattttgct
    13501 cgctccctcg acatggatat tcaaagtttt agtcaaaaag ccctgcttgc agaaaaacct
    13561 gtcgaccttg gttcacgatg tactgttttt atgaatagtc gcgtaaaaca ggcgcaaaaa
    13621 gaaggcgcaa ctgttgctga catttctgca gggctttcat attctgttat aaaaaatgcc
    13681 ctttttaaag taataaaact tcgaaaggct tcggatatcg gcacaaaggt tgtggtacaa
    13741 ggtggtacct ttaacaacga tgccgtccta cgtgcctttg aactcgtttc tggtgttgaa
    13801 gtctttcgcc ccgatgttgc aggtctcatg ggcgcctatg gtgctgccct tatcgcaaaa
    13861 gaccagtgga gcgaccttat tgcgataaag cctggtgaag attttattct cgataaggag
    13921 ccggtactat cctctcttgc aagcttagaa gaacttgaat cttttaaagt tgatttagag
    13981 cttactcgtt gtggaaaatg taataataat tgtctcctta cgataaacac cttttatact
    14041 ggtgctgaag ggcgaagttt tgttacagga aatcgctgcg aaagaggagc agaacttgaa
    14101 ggagggaaaa ctactgtcga tgccacaaat gcaaagaaaa atgccctggg cggcaaagaa
    14161 gacggtccct taccaaacct ctttgaatgg aaatataatc gcctctttcg ccattacaaa
    14221 ccgcttctca aaaaagatgc accacggggc gagattggta taccgcgcgt actcaatacc
    14281 tatgaaaatt atcctctttg gtttaccttt tttacaaaac ttggttttcg agttcgttta
    14341 tcgccacgct caacgagaga tatttatgaa ctcggccttg aatctattcc atcggaatca
    14401 gtctgttatc cgggaaaaat aagccacggt catatcgagg cattattaaa agaaggcgta
    14461 aatcgtatct tttatccctg cgcaccctat gagcaaaaag aagacgaggg agcaggaaac
    14521 cattataatt gccctattgt aacgagttac cccgaagtac ttcgaaacaa tatcgatgca
    14581 ctccgtcaag atgactcaat actttttctc aatccctttt tgcccatcga tcacaaggag
    14641 cgcttaaaag aacgcctttt tgaagagctc ggcgaaatac tctctctaag tcaagctgaa
    14701 ataaacaagg ctgtcgatgc tgcctggaag gagcaagata ctttcaaaaa agaagtgcaa
    14761 gaagctggcg aagatgctat tttcaatata attcgtcgtg gcggcatggg aattgttctt
    14821 gcaggtcgcc cctatcactt ggatccagaa attcatcacg gcatacctga acttataaca
    14881 ggtcttggtg ttgcggttct cacagaagat tccgtcgccc atctcggcaa aattgaacga
    14941 ccgcttcgca ttgtcgatca gtggacctat cataatcgct tgtatcgcgc tgccgccttt
    15001 gttgcggata tgccaaattt agaaatgctt cagttgacga gttttggctg tggcttagat
    15061 gcggtaacct ccgaccaagt tgaagaaatt cttgcggcaa aaaaccgtat gtatacactt
    15121 ataaaaattg atgagggctc aaatctcggt gcggttcgca tccgtgtaag aagtttaatc
    15181 gctgcagtgc gagaacaaga gagaaatcca aagaaaaaaa agaaaaagat ttctgcagcc
    15241 tatcaaagag aggtttttac caaggaaatg aaggcgacgc atacgatttt ggcaccacaa
    15301 atgtcgccta tacattttcg ccttatacaa aaagcctttg aatattctgg ttttaacttt
    15361 gttatcctac ccgatgttga taccgaggcg gttgaaacgg gcttgcagtt tgtaaataac
    15421 gacgcctgct acccttcaat cttggttgcg ggacagatga tagcagccct ccgctctggc
    15481 aaatacgacc tcgaccatgt cagcttgctt attacccaaa ctggtggtgg ttgtcgcgct
    15541 acaaattata tcggttttat tcgtcgcgct ttggcagatg caggttggga acatatccca
    15601 gtaatatcgc ttaatgctgt gggcttggaa tcaaatcctg gttttaaaat aggttttccc
    15661 cttttacata gggcttttat ggcactcatg cttggcgatc tactcatgcg agtactgtac
    15721 cgaacacgcc cctatgaggc aaaacctggt tcagccaatg ccctctatga aaaatggaat
    15781 aggcatattc aaaaacagct aaaatccatg tctatcttgg cttatcatcg catgataaaa
    15841 catattgtac gagattttga tacccttccc ctctctccca taaaaaaacc acgagttggc
    15901 gttgtcggtg agattttagt aaagtttcat cctacggcaa ataacgacat ttttagcaca
    15961 attgagcaag aaggtgcaga atgtgttgtt cccgacttgg cagatttttt gttttacacc
    16021 tttgcaacgg gtatttatcg ctatgaaaaa ctttcatttc ctaaaaagac aaaacgaaat
    16081 gcaaagttac tcgtatgggg aatggagctt tatagaaaac gcattaaaaa agagctttct
    16141 aggagtcgtc gatttaatcc acccacatct atttatgatt taatggaatc tgtcgatgat
    16201 atagtacagc ttggcaatat aactggcgag ggatggtttt taaccgccga aatggttgaa
    16261 ttgattcacg atggcgtgcc aaatattgcc tgtgtgcagc cttttgcttg tttaccaaac
    16321 cacgttaccg gaaaaggtat gataaaggag ttgcgtcgcc gttatcctga gtcaaatatt
    16381 tctgcaattg actatgaccc tggttcaagt gaagtaaatc aattgaatcg gctcaaactc
    16441 ttgcttgcaa atgcaaaacc aggacgacat ccagacgaaa aaaaatgaaa cgaaggcaga
    16501 tttgttttag tatagtattt ctctttttta gcgtgggctt tccagtctat gctgccttta
    16561 atccgctttc catatctgac tcaagttcta ttcgtgagga tatctactac acatggctta
    16621 cgagaagctt agatgaccta cgagaagaaa aagcaattat tcgtaaaaac gaggcaggag
    16681 actattttcg agtctctttt gtggaatacg aaaaagaatt tgcaattatc tgtaatccaa
    16741 tggtcgaaaa cgattttcca gaaaaaacat ctggctcttg gattttatat cgcgataagg
    16801 aaacagaaaa agcccttcgc attgaatttt attttacaca gagttctgag gtttatttta
    16861 cttttaaacc aagtggtaca aaaacctcta ttgacctgcg tatcttcaat gcctatctta
    16921 taaaagacca taatttttct ttgacattta gagattttta tacgctttca ataccgcaat
    16981 tctataccct ttcaaaaaac ctctttccct ggcatttttt cacacctgaa gagaagttat
    17041 acgatgcaaa tattcaaatc gcatcacaat tgcgcgaata ccaagacatg attgactttg
    17101 ttccaaaccg tgcttatgat gaaaatggaa agcctagtca gatttttgac tctagtgaaa
    17161 atctgcagtt aaacactcgc tataatgtag aaagcaatga aattttagaa gacagcgata
    17221 aactacaact aggttctttg ggctatgtaa aatggcttgt cgatggactt gtctatccac
    17281 aaattgcttc atacttaaaa atacaagcct tgaaaaaggc aacggttgat acgagtcctc
    17341 gttcacgagt agaaactttg agttcccgag aaaccatcta tgccgcactt gattggacac
    17401 gaaatttggc aacagcctat gcctcagtac agcttggaag ggatttagat ttttttacat
    17461 caggaacaga agtaaatcac tctttttttt cactgagctt tgattctcat ggcaaagtgc
    17521 gggatagcgc atatgtaaaa aattctggtt acagttcgca tattttaaaa ccgcttctct
    17581 acatgttagc tttaacagaa cctgagcgaa tgtatatcgc cgcgataaaa gattttcgct
    17641 ccgacactga ggaaggaaaa ttggacatag ggcgatttcg agatgcactt atttttttac
    17701 cttattttga tgcaaaacga gaatttcaat gccccgtctt ctatgcggga aaagaaataa
    17761 acctcgatga acttatagca aacagcgaaa atgacttttt ttctttagtg cgaataaaaa
    17821 ccgagcttag attttatcca ttaaaaaagg aatagacagg ctgccttccc tcgtgtacaa
    17881 tatcatatca tgaaagaaat taaggcaata gcatttgaca ttgacgggac tctctatccc
    17941 aactggcggc ttaatatacg agttctgccc tattttttaa agaatattcg attttttctg
    18001 gcttttaaaa aagttcgaga tatactgcac caaacagccc ccttgggaga tttttttgaa
    18061 tatcaagcac aattactctg cgatgttatg aaatgtgatt gtaaaagcgc aaaagaatat
    18121 attcatgaga ttgtctatga cggcttggct ccctatttta cggagataaa gccttttaaa
    18181 aacgctattg aaagttttgc tgtttttaaa aaagcaggtt ttaaatgtgc tgtcctttct
    18241 gattttcccc ctgaacaaaa aggagacata tggggagcga agcaatactg cgatgttatt
    18301 ttaggttctg aagaaatagg tgcgttaaaa ccttccatac atacatttgg ggtgctttca
    18361 gagaaattgg gacttgaacc ccatgaaatt ctttacgttg gcaataatat taaggcagat
    18421 atcattggag ctcgaaatgc aggcatgaaa acagcatatt tattgccctt ttggcgtcga
    18481 attttgggtt taaaacttcc acaagcagat atttcattta aaaactatcg ccaattacag
    18541 aaaattgtgt taaactagta aaaagattag tgttgttggt gggccaggat gatatttaat
    18601 attgttacta taggtatttg tgtaactctg atacttgctt ttaggcagtt agataaaaaa
    18661 gataaatccc tagaaaaagt gcgtaaatat tcggaaaaaa ttattggtga ttttgaagcc
    18721 tattttacgc aaaaaaaaga taatttaaaa gaagcgagtt tagaaattga tacaaaacaa
    18781 tcacatgcac ttgcttctgt aaaacgttta gattctatta tagaagaatt caataaaaaa
    18841 attgcagcac agacctctct tgttaaaacg ttagaacaac ttgagcaaaa aactgctcat
    18901 tacgatgctt taataaaaga attacttgaa atgtcagagc gtgttgaaga aaatctcatg
    18961 aagataaagg ctgagtctgc ctttattgca aagactcaag atcaagtaca tgccttaaaa
    19021 aaacaacaag actatctcga aaaacgcatc ccccaacttg aaaaagaatt taccgctgaa
    19081 aatgaagcga gcctagacgc gcttacaaag gggcttttag atacctttca taaggatata
    19141 gaaacgattg aaatttcaac tcgcgatgca ttgatgcaaa atgaaaaaat tttaacagaa
    19201 attaaccaat cgtatgaaga tgcttttgct gtggcaagtg aacgcgccga tgcaattgaa
    19261 gggaaggcct ttgataaact acgaaataaa gcgatagaaa gagttgaaaa agcaaatacc
    19321 tttttagaag agaaaatagg cttcctaaac acgacaatca agacaaagat tgatgaaaca
    19381 caaagcctca ttcatagctt caatgacaat tggcgaaatg aggcaaatga atatcttaca
    19441 acaatgcgct tggaactcaa acaaatgcaa ggtgaatacg aggagactgc cgaagagctt
    19501 gcagaatctt tgacagagta tagggatgaa agtgccatct tttttcaaaa aattagtact
    19561 gagctatttg atggcgagaa ggcacttgaa agcatgcgag ctgaaatgca atctgtaagc
    19621 gatcaaatgc aaagcgaaat cgccctctta aaaacgcagt tatatgattc gcttatcgga
    19681 ctaaaaaatg aaatagaagc tgaatcaaca gcatataata cagaaataaa agaaacattt
    19741 caaagcaata ttgatgaaat agaaaatgtg tttacaaata atctagaaag ctctaaaaat
    19801 tcttttaatg accgcttttc aagctttcaa actgaaattg aagcaaaatt cttgcgattt
    19861 acaggtgaat atcaaagctt gttagatact tttagcgatg atttctcttt taaaagcgaa
    19921 agcttggaaa aaggaattgc agatatagat gtaataaaac agagtttaga aaactcgctt
    19981 aaagaaataa gtgcaaaggt tctaggtgac ttttctgact tcacggatga gcaagcaagt
    20041 tctttgcaaa aacatgaaga aaactttgca aatcaaatgt ctcttatttc tacaacaatt
    20101 cgagggcttg aaaatgaatt gcaagaatta aaacaaagag cctatgacaa tgtgtcggaa
    20161 aaattgagtg ggtttgaaga ttccttcctt gaagatttaa caaaacaaag tcaaaggctc
    20221 aacgattctc ttgaaaactg gcaaaatgct ttcgatgcaa agcttgaaaa tcttgctctt
    20281 gaatacagcg atgaaagaaa agtcctagaa aacaaataca ccgaggaatt aaaagagaaa
    20341 atccaagcta ttcaagagcg ctttacaaac tatagcaata cacttgaaaa tagctttgat
    20401 gaaaaaaata aaaaatatca agcagatttc ttaggacttg atgagagatt ggttttattt
    20461 ataggtgaat acaaagaagc ttttgaaaaa acgcgaatag aggcagatgc ctatgttaaa
    20521 aacgaaatca cgcattttat tggctccatt gacgaacaaa taaaacgcta cgaaaaagat
    20581 gtggctcaaa gtctcgaaaa aatgactgat gacataagcg attcaagtca acaagcggca
    20641 gccttgctcg attcgatacg gaccgacttt tccgagtgga aagaaaggat gaacagtcag
    20701 tttgcagaag tgaaaacaat ttttgatgat aaatacgctt cttttagtga ggatgcacaa
    20761 aatgttttag ttgatttaga aaggatgtta aaagatgctg tttctacaag ttccgaatca
    20821 gccttagaac aatttagaga actagaagag aagatagagg ctatttcaaa tgagactgaa
    20881 tctgttatcc aacattataa taagcattca aacgatatcg tagatgattt taaagcgatg
    20941 tacaaagaaa tgcttgaaga aacagagcga aaaataaaaa cacagaaagc tgaggtcgaa
    21001 gagcagttac gacaagagaa aaaaattatt caagatataa aacttacaaa tgaaacaagc
    21061 caagaaaaaa tggttttgaa gatgcaaaaa gattccgatg agcttaatac aagccttgat
    21121 accatcgatc gaaagataaa acaatttata gcacaaacac agatttttga aaaagcagat
    21181 gttttaaaac ttgatttaga agaaaaaatt tctgaactaa aaaatgaaac cgcacaattg
    21241 gtgcagtatg aaaaaatgat acagagtctt gaaaaacaag tagaaaagct taaaaaatct
    21301 gaggaagaag ttaatcaaag ggtattgaaa tttaaccaag agaaaaaaag aatcgacgat
    21361 atggaaaaga actttaatga acttgtaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21481 nnnnnnnnaa aatcaaagat gtactggacc aggctgattc cttagatgaa attctttcaa
    21541 ctactgaaga gcgttttaat gcgatgcagg attcaaaaca ttggcttgca agtacagaat
    21601 ctcgcttaaa tgaaataaat aaagaaacaa aatcttactt aaagcttttg ggtgaggtaa
    21661 aaaaatctcc cggcacgcgc gattcacgct ctagtcaagg cttagtcttg gaaacacggg
    21721 aaaatgtgat aaagcttgca cgattaggtt ggactcctgc aaaaattgcc gagcgcttga
    21781 atatttctca aggtgaggtt gagctcatct tggaaacgca aggcgaataa atttcgatta
    21841 cacttttttt agggtttgat ttttaagttt cgcttttttc tagcaagcaa acaacatggg
    21901 cttcgatccc ttttccgcgt cctaccgggc caagtttttc gtgagttttt gcctttacaa
    21961 agatttgtga tgccttacat cctaagatcg acgcgattga ctggcgtatg gtctctcgat
    22021 gcacgaggag ttttggtttt tctaaaataa gcacagcatc aatgtttacg agacgccaag
    22081 atttttcttc tacctttttc catgcttctt gcaagagata tgcagagcta gcattttccc
    22141 aggtattgtc ttccggtgga aaaagctccc caatgtctgc aattccagca gcaccgagaa
    22201 gggcatcagt gatggcgtgt aagagtacgt cgccgtcaga atgcgctttt tcacccttgt
    22261 gaaacggaat gtgtacaccg cctattcgaa gcggtctttt tttgataagt tggtgaaagt
    22321 cccaaccaaa accaattcta atcatgctgt accgaatccc tttttttcca cgttttctgg
    22381 tatatcgctt tgataggtaa tttttatatt tttattgttt ccgttgacaa caaaaacagc
    22441 tttgtttgtg taatcggaaa atatttctga gtcatctgta tagtctttat ttgattgctc
    22501 tgcttttttg tgcgcttcga aaatagtctt ataagaaaaa ccttgtggcg tttgcactga
    22561 aacaatcgtt tccctttcta aatgctccgc aatacattgc ttttctgtat ctagtttttt
    22621 ttgtgtatca acactggcaa taacgggaac tgcggcacca tgggtgatag tttttgtgat
    22681 tatatcatgt ataattttct catctacaaa ggggcgggca gcatcgtgta tgagaactat
    22741 gtcgggtttt gttgcatact gtgaaagttt taaaagacct ttatatacag actcctgcct
    22801 tgtttttcct ccgatgatga attctggcga aagttttaag gcaatgcagg cgtcgagtgt
    22861 tttttcatta aaaaaggaag tcataccctg atatatttgt tttttaggca gcgttacgac
    22921 acaactttgc agcctatgtt ctgtatggtt ttgaaaatag cgcaaaaaag caagagcgct
    22981 cgctgctaac acagtttcgt cttgttcgat cggcataaat tcttttttga tagaagtctt
    23041 catgcgtgta gaatttcctg ctgctgttaa aacgagtgaa atgttcatat agagataacc
    23101 ttttgttttt ataagaaaat gtttagttag taattaatcc tcatcatcgt cgtcgtcatc
    23161 tacatcttca tcgtcgttta cgtcaaaaac atcttcgtcc tcatcatcgt catcgtcatc
    23221 tttttggctt gctgatagtg gctttgcttg ttgcattcca agaggttcga gttttgtgtg
    23281 tattaaactt tcaacctctt ttgctgtttt ttcaagagcg aaagatattt catcttcaag
    23341 gagttttttt gcagaatcgt agagttttcg ctctaaaata ggtaattctt taatcttact
    23401 tctatgatag aggcaacgaa caatcgttgc aatatcgtcg acactacctt tttttaataa
    23461 atcaaggttc atttgatagc gcaatttcca gtcgttcgtt ataggctcaa aatcgtctcc
    23521 caaagagtca agcgtttctt gtgctttttt tgcatctacg atagagcgga taccgagctc
    23581 atcgcatctt tctacgggta tcattactgt catgtcagaa gcttcgagat aaataacata
    23641 gtagagaagc atttgttctt taaatgcttt ttctttaatg tcaacaattt ttccaacacc
    23701 ttgacttgga tagacaattt tttggtttag ggcataagca gtttttagtt tactcatatt
    23761 cactcattat atcatagtta ttagttaatt gctataagca aattttttat cggacctctt
    23821 tctttaaagt agagatgttt taaaggatgt aaatcgaggg cgttttcgta ccagcctccg
    23881 agtatcgctt tgtcaacaat atttactgaa atggggtggg ataggggtat gacaataaaa
    23941 ctgtcgagta gctcttgttc tgcctcggca agtttttcat agcgtttttt agtatcgtga
    24001 atgccggttg cttcttgcaa aagattttca tagacagggc tagaccatcg gctttcattt
    24061 aaacttgagc cttttttgaa tagctctaaa aaagccatgg ggtcggcaaa gtctcctatc
    24121 caggtatagg taaacatatc agaattgctt tgcggtaccg catcgtaata gtcatagctt
    24181 ggtattttaa ttatatctgt acgtatgcca atttttttcc agctttcggc aaagagggca
    24241 acttgatctg catgatagtc tgtgtcgggt atagaaattt ctatacgttt ttcttcttcg
    24301 ttgagaccaa gggaatctaa aatagcttgt gcttcttcaa tgtcgctttc gtctacgccg
    24361 taaatctctg gatagttgta aagtcgcgat aagagtcttg tagctggaac ataaaatgct
    24421 tctcgcagtt tttcccaagg cagggcaaga agcagggcct gacgtgcttt ttgactattc
    24481 catacactcg tgttttgcat tctaaaaaaa taatactcag tgccgaactg tgtgccaaaa
    24541 acaagagagt cgttttctat cagttttgaa gcatcgaaat tgtcggcaag ccagtgtacc
    24601 ttgccaatat taaataagta tgtgttttct acttggtctt cagaaaagag gaagcgaatc
    24661 gaagggaggg caacattatc tgcatcccag taaaaagtgt ttttagttaa acgaagctct
    24721 gctttattta gttgactaat aacaaaggga ccgctttgta ttgttttatt ttcgtgtata
    24781 actgaaaaag catgatgaca aagaatcttt gcgaggtgtt ctgttgggtg tacgagtttt
    24841 ataacaagac ttccgtcatt ttttacttga attccgacct tacttgcatc gcctttcccc
    24901 agtcgatagg cttgtactcc ttcaatgcag tcaaggaggg aggcaaaggg agcatttgtg
    24961 tttggatcga tgagtttaag ccaagaatca taaaatgact gggcgcttat gggatcacca
    25021 ttggcaaatc gtgcattttt acgtatgtta aaggtccatg tttttttatc tcgcgaaatc
    25081 ttatatgatg atgcaagtgc tggaatcggt tcgagagaaa gggggtcata gctaaataaa
    25141 ccttcgtaaa gtccgctgag tatttgtgct tcactcgaat aactagaaag ctgcgggtta
    25201 aggtttagat gccccggaga ttgtattata agtaattctt cttgaatttt gcttatttcg
    25261 ttttcagttt tttgtgcaaa gagctgtgtg gaaaaaaaac tgatgaataa taaaaaaata
    25321 aaaccacgtg atagatattt ttgggcttta aaatgcattt gttataccta gctttttcat
    25381 ttgctcttgt tcttcaatga gagtaacgta ctcggctttt ctttgatttg tttttatgag
    25441 cgtgttttta agtgcagcta tttccatttt tattcccttt attttgtttt gttgtatggt
    25501 gcttatattt gctttataat agtcttcaag ggcaagcaag acatcaagca ttgtttggtt
    25561 ttctgccaat tgtgttacaa tgaattcaac aattttatca ttgcttaaaa gctcaatttt
    25621 tttgtaaccc ggtgtttttt tcatggaaaa ggatgtgtaa aagcgaactc gtttatgtaa
    25681 attacctaaa aattgttcga tttctatgtt ttctgttttt tgttctcgcg ttattggatt
    25741 tgtaatcgtg agtcgatagt gtatttttcg tggttttacg ttaaatactt ttcgaataaa
    25801 gctcgaaagt cgctcaaaaa aactagaatc ttcggattct aagagttttt tgttgtcttc
    25861 aagtttgctt ataatttgtg tcagttgtgg aacaaggcct gttaagattc tcactgaatc
    25921 catgagtatt tcttttgtat caattgtttt ttcttttaca actgtctcac ttttttccac
    25981 gcgtagtttt tctaagatat ctctttgtgc cttctctgca tttggtccaa agttttctac
    26041 gagaatttct tcaatgagtt ctgtgtaaaa gggttgtttc cccataacag aagtatatac
    26101 cttttttatt tgtgccatgt aagcttgtgt gtttaatgcc ggattcgcgt ttacgaaaga
    26161 cggatgagag aaaagtattt tgcgaatttc tatcttgtac atttctttgt gaaagtcaac
    26221 aagatttttt aaaatgccat ttataattgc ggttgatttc cctgcattgc ttatactatc
    26281 gtttacaacc gatatggcta aagagtcgga gccttgacga accgtggcta aaatttctgc
    26341 aagtccacga gcattcggtt ttgttgaatt ggttgatact gagttccatt gaaaggctgc
    26401 atttaatgcc gtgagttttt ttatacgctc caaattgagg cttgcaaccg aaaaagtgag
    26461 ataattggaa ataaaagtaa gagcagattc taagtctgag agtctcatgc ctattaccat
    26521 gttacgctca ttttcgttgt aaaaaccgtc atcgatttga ggaatgtcag atattttctt
    26581 gtcgtgttta taagggtctt catgaataga gcccttattc aagagggtgt ttataaggtt
    26641 ttcgacatag gcatggagtt ttctatactc ggaaagaagt cgaggtaatt cttcgccatc
    26701 gtaccacgcg agttttgcat caaaggcaga aaatagttct tttgaaaatg tttctggata
    26761 atccatgtta ttagtatcct atattaggtg tattttttca agtattttta taagtgaagc
    26821 gcttcctttt tttttacttg tataatagag attttttcca cttattgact attttttttg
    26881 tgaacagtat cctatttaca tagattgacg cagcgagtat ctgtggcaat aaaggaagta
    26941 aaaatgatac acgaaaaaaa tgaggcgctg gaaacaattc gacacagtgc cgcacatgta
    27001 atggccgatg ctgttttaaa actttttcct ggttctaagt atgctatagg accagcgata
    27061 gaaaatggct tttattatga ctttgattta agtcgcccca tcaaagaaga agaccttgga
    27121 gaaattgaaa aagaaatgcg ccgcattgtt agtggcaatc atgatttttc gaacaaggtg
    27181 ctttcaagag aagaggcttt ggactttttc aaagaccagc cctacaaaac agagcttatc
    27241 aatgatttag ccgaagatga acaaatcacc gtatacactc atgcggattt ctcagacctt
    27301 tgtcggggtc cccatgttgc aaatacaaaa gaaattaatg ggcaagcgtt taagcttatg
    27361 aaaactgccg gtgcctactg gcgtggcgat gttacaaagc ccatgctgac acggatttat
    27421 ggcacagcat ggcaaaaacc aagtgaacta aaggaatatc ttgccttttt agaggaagct
    27481 gaaaaaagag accatcgaaa aataggcaaa gagcttgatt tatttcatat cgatgatgat
    27541 aatcctggac aaattttttg gcatccaaat ggctggacta tttacaaaac acttaaaaac
    27601 tatgtttccg ataaaatcga aaaagatggc tatgttgaag tgaacactcc ctttgttatg
    27661 ccacagtcgc tttggttgcg ctcagggcat tgggaaaaat atcaggaaaa tatgtttgtt
    27721 actgaaagtg aaaaacgact ttttgccctt aagccgatga actgcccagg acacgtagaa
    27781 atatttaaaa caaagacacg ttcgtataaa gatcttccct tgcgtatggc agagtttggc
    27841 tcttgcacgc gaaacgaagc atctggtgcg cttcatggta ttatgcgcgt tcgtggtttt
    27901 gtacaagatg acggacatat tttttgtacc gaagagcaaa tatcaagcga agtggtaaag
    27961 ttttgcattc ttttaaaaga tatgtacaag gattttggct ttgcagatga tgcaattatc
    28021 gtaaaattct caacccgtcc agaaaaacga gttggaagcg atgaaagctg ggataaaagc
    28081 gaagctgctc ttgaagaagc ttgtaaggct gccggtttag cctatgaggt gcaggaaggg
    28141 gagggtgctt tttatggacc caaacttgaa tttaccctcg tcgatgcgct tggacgacat
    28201 tggcaatgtg gtactattca agttgaccac caacttccta gtgctgatcg cttacatgct
    28261 gattatatcg gggatgataa tgcaaaacac caggtggtaa tgttgcatcg agcggtttta
    28321 ggttctttag agcgttttat cggtatcttg ctcgaaaact ttgctggtgc ccttcctgct
    28381 tggctacaac cgcaacaagc tgttgttatt cctgttgccc cgacagttga aggctatgca
    28441 caagaaattc aagagcttgt aagagcagca ggaattcgct cgaagataaa cctcggaaat
    28501 gatcgaatga atgcaaaaat acgggctgct caacttgaaa aaatacccta tatgctcgta
    28561 gttggcgaaa aagaaatggc agaaggtact gtcgctgttc gatttcgcga tgggcgagga
    28621 caagaggtga tgacaaaaga agcatttatt tcctatgcaa aagataaaat agataatttt
    28681 tttgtaggaa tttaaaaaaa agtaacgctt ttttttaatt caagtgttat aatagtgaag
    28741 agtgtttttt atatgaagta aaaaacagct tgatacgttt gcgttgcaaa acgattgaga
    28801 aaaatctgtg aaactcgtct atcggtggcg agataaagtt taggactttt ttataggagg
    28861 aagtatgaaa aaggttttaa gaacgttttt tgttattttc gctgtacttt ttgtggcatc
    28921 ttgtgcttca aacaaaggga gcagagatgt tggaattgaa ggtgaaaaaa gacccgattg
    28981 ggtaaaaaaa atgggtatga gcaatgatgt tactcattac gaagtaggct atggaaaaat
    29041 gtccaattat gcaacttcgt taaaaaaagc agaagctgat gctagaaata aaattgcgtt
    29101 ttggattcaa acagatgtaa acactgtttt aaaaacctat acccaagatt caggtatggg
    29161 cgataacaga gaactcatcg aattcatgga agaagtttct aagcaaaccg caaatttctc
    29221 tctttctgga gcacaaattg aagatgcttg ggaagatgca gatggcggag tctatgttct
    29281 tatgtctttt cccgtagata aggctggggc aacattagat aatcaattaa agacatacca
    29341 aagaagtgaa tctgctgctt ttgcagaatt taaagcacaa gaagctttta agcaaatgca
    29401 atacgaacaa tcacaaggtt tctaaggcgt gaaactgaaa taaaaggctg ctgaaaggga
    29461 aaaactttcg cagccttttt tttatttaaa tagtttaaat ctttattgtt ttatttactt
    29521 tgcggtggaa aaaaacaacg aaccttaagt gaaaaaaggc tgttttggag gggagaagag
    29581 agttctgtca taagatttgc tactggataa aaggccttga agctgagtcc aaaactactg
    29641 ccattttcta aaacccaatc ccatgtgatt gttgcttcgg gatagaggta gcgaaaatct
    29701 gaccaaaaga aatcatttat tttgccgacc ttgtattctt cgttttcagg aagtactgct
    29761 gttgcaaagg cgactctgct gagaagggcg atgccaaggc caaaggaaaa gacattgttt
    29821 tcagttgaaa ttttatacaa taaaggtatg tccaataaaa aatggagtgc ttgtacgtct
    29881 cgtttttcca ctgctgctgg agaagccctg tcgtttaagt agaggtagta aagcgaccaa
    29941 aatcctagtt cggggtttat ggcgaggtct ttgtttagag aaaaagaata gccaaggcca
    30001 agactaaatg agatggggga gggtgcggtt tctaagccat ctgtgtttat ctgcaatgta
    30061 ggacctgctg cgagaaaaag ctttccctgg cgatcagcgg cgtttgaaaa aaggctcaaa
    30121 ctaaaacagc aaaaaaaggc gaatgcgtat atttttttca tatctacctc tcaaattata
    30181 ttgtatcttc tatcattgag cataataaag gagctcctca aggtcaactg tcaaaaaacc
    30241 tgagcgcgct gtttcgtaaa aaaaactttc ttcccaagca atatatagtg tggagccaga
    30301 aacaccaaaa tctccgtagc taaagtgttc tgggagtctc ggtaggcgaa aggcgagagg
    30361 ttctccttgg ttgagaatat ttttgtttgg gagggctcca acaaaaaaac tcgtcccatc
    30421 tgcaaaaaca gcaagggaaa agctgtctgc aatcaaagca tttgatgcaa tgagtttttc
    30481 atcgcttata gaaccgttta gatagcttag ggagcttcct gcgtttgttt cagaaaccat
    30541 gcaataaaaa ggataatcgg gtggaagttt tttatataag tcctgtaatc gtttgggcgc
    30601 cgttgaaaaa ggaaggggtg ataaaaactc acggtattca gctgttgata attgtttttt
    30661 ttcaaggtgt aagcttcgtt ttgccgctga ttgccgagtt atgggttcat aggaaaaaaa
    30721 gtttagataa tcaaattgtg tttcttcact tgattcagtt ttgagggcag caatccaaaa
    30781 accctttgcg tattgtaggt ctgttacttc tgttgcatcg ctaattccta aatcgcttgt
    30841 atacaataag ggtaaaaaaa tagaggttcc agggtgaaat tgaactaaaa agggtagttt
    30901 ttcttttttt tctgtgagtc gtgaaaaaaa ggagttttta taggtatgaa aaacagggaa
    30961 gttgtcgata caaacgaggg ctcctgcagt gagttgtgaa aaaagttgtt ggtcagatgc
    31021 gaggattgct tgttttggat gccccggttt tgcaatgaga atgccgactt tatttacggt
    31081 aaaaaagagg tcgtagtctc ttttgtcatt gcgtatagaa aggctgctag aaatacgctc
    31141 ggtttctgtc catggttttt tgataaggcg gggaacagca cttgggtgat cggtttcgta
    31201 aaaggagttc ccgctaaaat aaaaccattg gtgtgttttt tgtgttttta tctggcttgc
    31261 acgaagtatt gcgtcgctcg tttcaagctc tctgcctatt tgattttgct tgcaagaaaa
    31321 aataaaaaaa cagagaaggc tacttataaa aagatttaga aaaagcgtga gaccacgggg
    31381 taaaaaatgc ttactgaatt tatacaatct attacacatt ataaaatagt gtaaagcttc
    31441 agagctttta tggcaagagt ttttttattg attttgaggt gaaacttcct tctgcgatag
    31501 cagttgccat aaggtctata tcaagtaaaa aggggtcgct ttgtttttct aaactttcta
    31561 cgagtttttc ccaattaaag taagatgcgt atgctatggc tgcctttgta tcgcttgcgc
    31621 cttgtatgag tttaaaaaac acgagggaag cgactgtgga tgcttccgat gcttcttggt
    31681 caaagggtgc tataaagccc cagggttttt ctgcgagtgc tttttcaaag ttctcgcttt
    31741 tttctaaatc acaagagaga agaagggctg tgcaatgttc tgggggaata gctatttgga
    31801 ctgcctcata gtttttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaag
    31921 ggctgtgcaa tgttctgggg gaatagctat ttggactgcc tcatagtttt tcgtcgcaat
    31981 gtcgagctga atgagttttc cttcgctatc aaagtacgag agcgtccaca cgaggtcttt
    32041 tggatagctg atatatcgca gtggctcaaa gtgaatgcaa ttttcgccag gagctgaagg
    32101 gagaaaatcc accttgcagg cgacgaaaaa gaggaaaacc aaggataatg ataaaaatgt
    32161 tttgttcata tcttattatt gtcgctttac cttagttttt attgttttta taaaaagatt
    32221 tttattttta aaaaatgtgg tgcaagaaaa tttttgatta aaccgcaaga aatagcaata
    32281 aacgacgagt ttttcaataa atttcatatt taatacaaag aattcgtttt aagggcttga
    32341 ttaaaaaaat atatttttat atacttgtat tattagcgaa ggctagtaat tatttagagc
    32401 ataatgcatc ttttccgcag gtgtaaatac gcaaggcgtg tttgtatttc gtttggttta
    32461 agacgaaaga aatgaaaagt aagattctac tttgtagatt ttatataatt gaaagttttt
    32521 caatttggtt tggttttttg cggttccgat gcatcttatg taaaggaaca tatggaaact
    32581 atctcatttg aagaccttgg tcttgatgct gcaacattgg ctgcagtcga acaaaagggt
    32641 tttgaaagcc cttctcctat tcaagtattg gcaattcccc ggctattaaa tggcgaagca
    32701 aacgttattg cgaaagctcg taccggtaca ggtaagacgg cggcctttgg gcttccgctt
    32761 gtgcaaagta ttcgtagcga tctcggtaag gtgcaagcgc ttatactcgc tccaacgcga
    32821 gagcttgctt tgcaagtggc aaaggaaatt gaatctttat caacagggaa gtatcctcgt
    32881 ctcacgacgg tctatggtgg acaatccatg ggcgaacaac ttcgcagtct aaaaagaggt
    32941 gtggagattg tggttggaac tcccgggcgt gtaaaagatc atatcaatcg tggttcttta
    33001 aaactcgaag atatctcgta ttttatctta gatgaagcag atgaaatgct tgatatgggt
    33061 tttatcgaag acattgagtt tattttttct aaagcaaatc aagactctcg tgtgcttctt
    33121 ttttctgcaa caatgcccaa ggaaatttta aaaattgctt ccactttcat gggcgattat
    33181 gaaattgtag aggaagagga acaagaagag gcaccgcttt taacggaaca aaaatattgg
    33241 gtggtgcgcg agggcgataa aaccgaagct ttggtgaggc taatcgatat ttcaccggag
    33301 ttttatgggc ttgtttttac acaaacaaag ctcgatgcag atatgctttc tcggcaactc
    33361 gacgaacgtg gctatgaggc tgcggccttg cacggtgata ttccgcaagg acagagagag
    33421 aaaattttat ctcgttttcg aagcaaaaaa acaaaaatct tggttgcaac tgatgttgca
    33481 gctcgcggca tagatattga aggtttaact catgtggtaa attatgcgct tccctttgac
    33541 ggtccaacct atgtgcaccg tatcgggcgc acaggacggg caggtgcaaa gggtttagcc
    33601 tttaccatgg tgaagcctga ggaaagacgg cgacttgagt acctaaagca ggttgcaaaa
    33661 aaagcaacgc agggtgagct taaagaagaa gtgataccga gcatagagca ggttgttgac
    33721 gtaaagaaaa cacgcctttt tggtgatgta aaatcgagtc ttaaccttac tcaaggaccc
    33781 tctcaaacct tcctcacaat ggcagaagat ttggtgggga cagaggatag cctctctgtt
    33841 cttgcttctg tcttggaaaa aaactatgga aatctcatag atcctaagcg ctatggagct
    33901 attcgtacgc cacaaaaacc ttcccttggt actcgttctg gcgatagaga agcatcaaac
    33961 caggtgcgga tttacgttca gctcggtcgc aaggacggtt ttaatccacg agaaattgca
    34021 agttatttta gccagctttt gggtattccc caacgaatgg ttgatagaat cgatactgcc
    34081 catgccttta cccttttgag tcttcccaaa aaagaagctt tagaaatcat agagcgaaca
    34141 aagtgggata agtccttacc gcatatgcat ctcgatacaa aagaaaatgc ccgtgttgat
    34201 agggataatt ttggtaaccg tggttctcag ggacgaaatg gtcgccgtgg aaattctttt
    34261 gctggggaat cagctcgtgg ctctcgaaga gcgagttttt catctgcaaa ggcaggcttt
    34321 aaagaaggtg gtggaagaaa gaatgcgcat ccgagggatt attccagtcg aggaaaaacg
    34381 agctcggctg ccttgtataa aaaagcataa tttcttatgt cgcttaactg gaaagagata
    34441 gagcttgtac tcgaagaact tgctatttct ggaagctttg tacaaaatat tgtacaaccg
    34501 agctttgatg cgcttgccct ctacctttat aaggagggca gggcgcaaac gctttttata
    34561 agccttgcct ctggttcctg tcgcctacat gaaacacggc ggaaaattcc caagaaccca
    34621 aaaccgctac gctttatgga gctcctaaaa aaaagaattt tgggggcgaa aattgtcgat
    34681 gcaaagcagc tgggacaaga gcgaattgtg cagctggaac tggaaaaaaa tgatgagcga
    34741 ctttttttat ttattagact ttggagcggt gctgcaaata ttcttttaac cgatagcgat
    34801 tttggcatta tcgatgcctt ttatcggcgt ccaaaaaagg gagaaattac agggcaaata
    34861 ttttctctcc cgctgccaag agagggggaa aaaggtaggg actatgattt gcgcagtttt
    34921 gaaagccttg cggatttcgg actcgatatt agttcgcttt ccttaagtga aaaaattgaa
    34981 aagtggtatg agttttgcgg ggcgcagctt tctgtagaat ccttggtcga gaaggttgaa
    35041 aaacattttg cccaaaaaat agcggctata gagaagcttt tacaaagtat agaagcaaaa
    35101 aaggaagctt tttcgaaccg tgaagtgctt aaaatccgag gagatttaat tctttcaaat
    35161 gcccatctca tagaaaaaga gcaaaaaata ctccgctgtc ttgattatac gacaaatacc
    35221 gaaatagaaa tacttttgga ctctacaaaa aatgctcaag aaaatgcaaa tgaattgtat
    35281 cggcaataca aaaaagcaga aacaggtttt gaagctttag ctatagaaga aaaaaagcta
    35341 gaaaaagaaa aacaggcact ttttgaggaa aaggaagcaa ttcttttgga aggaaacccc
    35401 ttgcgacttg agcaagtttt acgaaaaata gaaacaccga agcaagtggc tgaagtagaa
    35461 agaccggggc tttggtttga ttttcaagac tggacaatta ttgttgggcg aaatgcaagg
    35521 gaaaacgacg agcttttacg ccactatgcc cgtggcttgg acacttggct acatacccga
    35581 gatgtacaag gtggctttgt ctttataaaa aataaacccg gaaaaacaat acccctgcct
    35641 gttttaattg ctgcaggaaa tcttgcggtg tatttttcaa aagcacgctc agaagcgagg
    35701 gcagatttat attataccca ggtgaaacac ctaaaacggg ctcgtggtgg aaaaaaaagt
    35761 gaaaagtcta gtttgacggg aaaggttttg cctagtcatg aaaaaaatct ttttattcag
    35821 cttgatacaa ggcttttgca agagcttggt ttatagcttg attcttttta atttctagac
    35881 tatttcatta gaaaagtaaa ataaaataaa acgcttttgt tgtaactttt ttcttttttg
    35941 tctgttatat aagtactatg atcgcaagaa aacatactga gcacagctga agcccgagaa
    36001 gttcctcctt tcttctcggg tttttctatt ttattgattt gatatcttct tgtattgcta
    36061 ttatatcctt ttctaattaa aataatcccc atggtagaaa aatcatttga aaatatatca
    36121 cgaaaagttg ccttagcttc ctgtgctgaa tacaacgaaa aaagcgtttt agaaaaaatg
    36181 cgccaattag ttatagatgc gccacctcct tcggttgaag ggaagacagt ccttctcaag
    36241 ccaaatattt tatcgccaaa aaaacctgag tttgccatat gtacacatcc tgtggttgtt
    36301 gcatcagcgg taaaagtttt tttagaactc ggtgccaaaa aagtttttgt tggtgaatct
    36361 cctgctgtcg ccccctctct cgtagccgca aaggcaagcg gtatgttaga agcaatcgaa
    36421 aaagctggtg cggaatgggt ggattttttt gagatgcaaa ccctgcattt tgaaggtgga
    36481 aagcttataa aatcgataga ctttgcaaag ccttttttcg aagctgatct cgttgtttcg
    36541 ctttcaaagc taaaaagtca tcaactcatg gcatatacgg gtgcaatgaa aaatcttttt
    36601 ggactcgttg ttggtctaaa caaggcacaa atgcattatc gattttctga taaaaacgac
    36661 ttttcaaaat atttaactga ccttgttgtc gcttctggtt cgcgatatgc tataatggac
    36721 gcaattgtgg gcatggatgg ccctggtgga cctggcaatg gtgatcctgt tccgcttggt
    36781 tttcttgcag caagtcaaaa cctccttgcg ctcgattgga cttgtgcaag tatggtcggc
    36841 tacaagcctc aggatattct aaatctttcg gatgccatgt ctcgtggttt atggcttaaa
    36901 gatgcaagcg aaatagagct tgttggtgag actgtcgaat cgatcggtcc gttgcaattt
    36961 cgcacggtaa ccgaaacaag tggtttaaca agtttgcaaa aaatgctgcc tgattgggta
    37021 aacgggcttg caaaaaaaat ctttgtaaaa acgcctgctt ttcagcatag cacttgtatt
    37081 cgctgtgggc gttgcataga aatctgtcct ccacaaacgc tttcttttga ggatgacgaa
    37141 aaatccaaat ggcaaaaaaa agtggttata gataagacag attgtattca ttgtttttgc
    37201 tgtcatgaaa tttgcccggt tgatgcaata caattaaaac gctttcaata aattatttta
    37261 aaaaagcatt ttcgttttag cgaggcaaaa ataaaaaatc aataaaaaag gtgaggattt
    37321 taagtatgaa aatttcaaaa attatagata aaaaaaagct tcttatttcc tttgaaatat
    37381 ttcccccaaa acaagaggca aattttgact ctgttatgca gacagccttg gatttaggag
    37441 gattaaatcc agattttatt agtgtaactt atggggcggg tggaacaacg cggtcataca
    37501 cagctgaact tgcatcgagc ttgcaggaaa aaacacaaat tccgtctttg gcacatttaa
    37561 cttgcgtggg ctcaggattt tcggatattc agtctattat tcgagagctt aaagagaaaa
    37621 agatagaaaa tgttttggcg cttcgtggcg attatcctca gggagcggat gcttcagatt
    37681 taccgggaga ttgcgcctat gcctccgacc tcgttcgtat tcttaaaaaa gaggctgact
    37741 tttgtatagg gggtgcctgt tatccggaag ggcatcctga aagtgaaaat cgcttggagg
    37801 atttgcaaca tttgcaatac aaggttgatg ccggtcttga ttttttaaca actcaaatgt
    37861 tttttgacaa tgaggttctc tatagttttt tgtatcgcat gcaatctaag ggtatgaagc
    37921 ttcctgtttt tgcagggatt atgcccgtca caaatgcaaa acaaataaag cgaatgatta
    37981 cactttcaaa tgcacatatg ccacaaaaac tgcttgcctt tgtggatcgc tttcaagata
    38041 atcccgaagc gatgatgcaa gcgggtattg cctatgctac tgagcaaatt atcgacttga
    38101 ttgcaaatgg tattcgcggt gttcatattt attcgatgaa taaacccgat attaccgcag
    38161 cgattatgca taatatttcg catatcgtgg aagcggtaaa tgccgaagcg catgtttaaa
    38221 aaaggcagga caagtatatg aaacaagact ttcgactttt tttagagaaa tctctaagag
    38281 aatcgcggcc gattattttt gacggtgcga tgggtacgat gatacaagat tctggtttta
    38341 cagattattt acttccagaa gaattgaata gcaaaaagcc cgatttaata cgcggtattc
    38401 atgaagccta tcttgcaagt ggtgccaatg ttcttaccgc aaatagtttt ggttcaaatg
    38461 gtataaagct caaggacgct tccttttctg cagctgaggc aacgactgct gctatagaaa
    38521 atctgcggtc gcttatagat gcacgagata ggtcaaggtt caagcaagaa gtctttgctg
    38581 ccctcgacat agggccgacg ggacaacttc tcgcaccaat gggtcgttta agctttgatg
    38641 aagcctatga ggcttttaaa gagtcggctc ttgcagcaga aaaggcggga gcggatctag
    38701 ttattataga aacctttagc gatttgtatg aagcgaaggc ggctatcctt gcagtaaaag
    38761 aaaatacttc gcttcccatt gtggctctta tgacctttca gtcaaatttt cgaacgctca
    38821 ctggtagcga tgtagaaacg acagtctgct atcttgaatc gctcggcgtt gatgcccttg
    38881 gcttcaattg cggtggctca cttgaagagg caaaaacttt agcaaagctc tttctcgaac
    38941 atgcaaacct cccccttgtt tcccttccaa atgctggact tccggtcatt gaaaaagggc
    39001 aaacgatttt caaggttggg gcgaatgaat tttccaaggt gcaaaaagaa atcgcaacca
    39061 tggggttttc tcttctaggc ggctgttgtg gcacgaagcc agagcatatc tcagcgctcg
    39121 ttcaagactt gcagcatttt tctataccca aaaataatag acctagtcaa aggcgccgca
    39181 gttcactttg ctctgctgaa aaaacagtct acatcgataa tagttttggc tctacgggtc
    39241 cagttattat tggggagcgt ataaatccta cgggaaagaa aaaacttaaa gaagcccttc
    39301 tttcaaatga tatgtcctat attttggatg aagctgaaaa tcaaatacaa gcaggaagtc
    39361 atatcctcga tgtgaacgtg ggacttccgg ggctcgatga aaaagcaaaa atgcaagaag
    39421 ttgtggcagc tttgcaaaaa aattatgcga ctcctttgca gcttgattca agtgagccag
    39481 aagttttgga atatgcaatg cgttactata acggaaaacc tctggtgaat tctgtaaacg
    39541 gaaagcaaaa aaacatggac gacgtttttc cacttgtaaa aaaatacggc gccctcgttg
    39601 tcgctctttg cttggatgaa gatggaattc catccactgt ggaaggaagg ctctctattg
    39661 cgaaaaaaat ctatgcagaa gcgcaaaaat acggcatcag agcagaggat attttattcg
    39721 atagcttaac cctaacccta agtagccagc aagaagaagc cctcgtaacg cttgatgcaa
    39781 ttaaagctgt caagaaagaa attcccggag caaaaacggt gcttggcgtc tccaatattt
    39841 ccttcggcct tcctcgccgt gatatcataa acgctgcttt tttttctatg gccttgtatg
    39901 caggtttgga cgcttgtatt atcaatccat tgtctgaaga aatgatggca agttttaatg
    39961 cctaccgaac cattgctgcc tttgatgata aggctttgca ttttatcgaa acctatagcg
    40021 gtacgcaaaa aatatcctcg agtatagcca ataaagctgc aactaaagaa gacctttctg
    40081 tgtctaaaga aacagaacaa aaaagccttt ttgcaaaaga tgatttacaa acaattatca
    40141 taaaggggca ggttgataag gctgagtcct gtgtggaaaa attgttagaa gcggggaaaa
    40201 catcccttga tataatcgat ggctgtattg ttccagctct agacattgtc gggaaagaat
    40261 acgaaagcgg aaaaaagttt ttaccgcaac tccttttgag cgctgaaact gtttctaagg
    40321 cttttggtct cataaaggct gaacttgcaa aaacgggcat acaggatgaa gcaaagggga
    40381 caattttaat cgcaactgtg gaaggcgata ttcatgatat tgggaaaaac attgtaaaag
    40441 cgatgctaga aaactacggc tatgaggtgc tcgatttagg aaaagatgtg agtgcagaga
    40501 ccatttgcga tctggctgtc gaaaaaaata tccgccttat tggcttgagt gcattgatga
    40561 caacaacagt actcaatatg gaaaaaacaa ttaagctttt gcgcgaaaaa gaaaaagaga
    40621 tggcagaaaa atttaccatt atggttggtg gcgctgtgct tacggaagac tatgcaaaaa
    40681 caataggagc tgatttttat gcaaaagatg cccttgcttc agtgcaaatt gcaaaaaaat
    40741 tttttggaaa ataaagaatg aaatgtgaag aagatgagtt cccgtcgcta aaaacatttt
    40801 caatagcgcg cgcctatttt tacgttatac tcaatcctaa atctctctgg gccttgcttt
    40861 gttacttgcg tacggtactt tgcgattttt tcttactcca gttttctgta aagtttggtt
    40921 ttaagaatct agaaataatc aatgttgacc atggtttaga cgattctgtt ccttttgatc
    40981 caacaaaagt tcatgtttat ttagattttg taaatttttg gattcgaccc atgagtttta
    41041 tgctaaaacg ttttggtgta aaaaaagcaa tcccctattg tgctcgctat cttaacgcta
    41101 ttgagcgttg ttattctgag gcatctcgca tgtaccgttt tagaatgtca acgacgaatc
    41161 gccctcctgc ggatgggaga aaaggttttc gcatgattca tcttcttgac ccacactatc
    41221 tttgtgttcc cagtttacac gtagctattg taaatcttgc ctataatttt ttccgtgatg
    41281 catttacaga ccttggaatg gaaaaagaag agcttacttt ttatacgagc gaattatatg
    41341 ctggagcaat agaaataaca gagacggtac tttacgtaaa acagcacagt gtcaactgta
    41401 tacctgcagc cttgtacatg tgtttattta tcttacaaga tcaattttct atccctgatt
    41461 cagtacgttt tattgaatcc ttatttctag atagcacaga tataagggca gaagatgttg
    41521 aggcaatcca agaccacatc ctatttttgt ttgaacaatt attgcttgaa ggaagtattg
    41581 aagatgattg gactgcacct gttaaaagat ggatattgaa ctatgcttct ccttgctcag
    41641 agaaatacgc ctagagcaag gaataattta ttcgtcgaag tcttcgggaa tatctgcaag
    41701 ttttttaagt tcaactattt cttcggcaag catgagtgtt tttctatttt gttttaacaa
    41761 gcgctgcgcc aaaagttttg agagaaaaat accataatgt gggttatttc taatgatttg
    41821 taaaaatgaa acctttggta tttttatcag tcgcccttca cctatcgata aaatagtagc
    41881 gctccttcta tcgtttaata aaaacgacat ttcgccaata aacatgtcgt ttggagttaa
    41941 gacagaaata agttttctat ttgtgtatac cgcatatcga cccgaaacaa taaaatataa
    42001 atcgttcgta ttatcatttt cgtagcaaac aacttctttg tccttgtaaa cttcagattc
    42061 aaagggtttc attatgccag gaacggtgtt tgcttcgtct tggagattca aaatatcaaa
    42121 tgtaacttca tttccttttt cattataggt gagagagtct actaaacttt gagatatttt
    42181 catgccacgg ccatgagtat ggtccgtaaa ttctttttca agacgagctt tccaatcgaa
    42241 gccgtctcca tcatcacgaa ttctaaatcg agatttattt ttcccaataa tgtaggatat
    42301 ataaattttt ttatttatat attcaggaag tttgtttttt ctataaatga gttctagaat
    42361 attgcctcca ttattgagcc attttgtttt ctcttcgtaa gagatttgta agttaccgtg
    42421 ttctagagca tttgttaaaa gttccatcaa ggttgtttgc aacttgtatc ggtcatcaga
    42481 gctaattcta tttgtattat ataaataact gacgagaaag tttgtataca tgcgtatgtc
    42541 gagggggtcg cttccacaaa caaaggagcc agtttctctt ccacctatgc gctcttgaat
    42601 tccacgatta aaaagaaaat gttggttgct ccataaaatg cgtaaaagtc tgtcaaagtt
    42661 ttcgtgaaag cttttaactg tttgaatgac aagaatgttt gagtcttttt tttcttcgag
    42721 cttttgcgct tcagcattgt ttgcgactat tgcaattatt ccaccgttgt ggagccatgg
    42781 atcggcatga atttcattta atatgttatt tgcatctatg ttattttctg aggtaaaatc
    42841 taaaactttt atttctggca attcataaga gacatagctt aaggtttcat cagttgttga
    42901 taaaaaaaca gcatcaaagt aatttgcata ttttttacaa gctgcacgaa cggttttgag
    42961 tactccttca tttgtagagg caactaaaat ttttttcata tatctttcct tttttataaa
    43021 atatgtgtat ttacaacact attattatat cacagtttta agaaatgaca gtaaaaaaaa
    43081 tgagttttgt ttttatttcc cgtagaatgt gttttcaaaa tctttcaaaa ttgtttcggt
    43141 atttgggcgc gcatctatcg aagtgatgag tttttctttt ttatagtagg caataagagg
    43201 ctctgtctga tctctataga cttcgagtct atgggtaatg gcatcttcct tatcgtcatc
    43261 tcgaataaat agttcagaac cgcatttgtc acaaattcct tcttgttttg gaggcataaa
    43321 atctttatga aagttttgac cacaagattt gcaaaccctt ctaccagaaa gtcttgcaat
    43381 aacatctgca tcttctatat taaaatttac tacatggtcg atgagaatta tttttgcgag
    43441 ggcttctgct tgaggaattg ttcgaggaaa gccatctaag ataaagcctt tttttgcatc
    43501 ttcttgtgtg aggcgctctt ttacgagttc aatagtgatt tcgtcgctca ccaattctcc
    43561 tgcatctata atcgattgta cacgaagtcc taaggctgtt tttgacttaa tcgctgcacg
    43621 gaaaattacg cctgttgaaa tatgtggaat attgtgcatt tctgctactt ttgctgcaag
    43681 agttcctttc cctgctccgg gaggtccgag aaatataaaa ttcatagttt atccttatta
    43741 tgtaaaatcg agctacatgc ttcgatactg tgttttttac actataacaa aaaaaacatt
    43801 atctcacaag atagttttct tagtttttga atcatattta gagctaaaag aggtttttac
    43861 tttttgtttt ttttaatatc gagttttgcc tcatagcctt ctgtgcccgg tgtatagaag
    43921 gttttcccaa ggagctttgc agggagatag tcttgcttta cccaatgccc gtcgaaatga
    43981 tgtgggtaga ggtagccctt gccatgtcct aaacgctccc catcgcgaga tgcatctttt
    44041 aagtgatttg gcacttcttg gtcttctttt tcaacttctg caagagcttc aaaataggct
    44101 tcgatgctgt ttgattttgg gcacgttgca aggtagaggg cggcatgggc aagttgatac
    44161 tgaccttctg gaaaaccaat ttggtcaaag gcttggcgcg ctgcttgcac gacaagaagg
    44221 gcataggggt cagccatccc aacgtcttcg cttgcaagga tgagcatgcg cctaaagata
    44281 aagtgtggat cttcacctgc gcgcaccatg cgagccagcc agtagagggc ggcatcgggg
    44341 tcgcttccac gcaggctttt tataaaagcg gagataatat cgtagtgata gtcgccgtct
    44401 ttgtcgtaga gaattgcttt tttttggatg ctttgttcag ctgttttttt tgaaataaaa
    44461 atctcgcttt caagcggtgg gaggggctct tcttcatatg gcgcccaaat agcaggtgtt
    44521 gtttctatag caagttcaag ggcattaaaa aggcttcgta ggtcgccatt tgcggttttt
    44581 ataagatgtt cgagtgcgcc tttttcaaaa accaccttcc atcgaccata gccgcgctct
    44641 ttgtctttga ggcaagcatg ggcggcatct tttagatttt cttctgagag agattttaac
    44701 tcaaagacac ggcttcggct taaaagagcc ttgtttactt caaaaaaagg attttcggtt
    44761 gtagccccga tgaggcttat cgtgccgttt tcaacccaag gaagaagtgc gtcttgctgg
    44821 cttttattcc agcgatgtac ttcatcgaca aagagtatgg tttttttgcc atagagtttt
    44881 tgggttttct cggcattttc tatggcatct cgtatgccct gcactccaga aagaactgca
    44941 tttaagctca gataattgcg ctttgtttgc tttgcaataa tatgtgcaag gctcgttttt
    45001 cctgttcctg gcggtccata aaaaataacc gagctcagtt ggtcaactgt tatagcccgt
    45061 cgcaaaaggc agccttcccc gataatatgt tcttgtccaa aaaactcatc gaggtttctc
    45121 ggcctcatgc gggatgcaag aggaccctca tttttttctg ccctttgcgt aaacaaatcc
    45181 atttttttat gcttactcta cgttccagtt tggacgatct ttataatagc cccaggtatt
    45241 tgttttttta ctcgttgttt tgtcaactgc aaaggcgtag atggcatctc caaaagagta
    45301 gagtgttttt atttctaaca ttgctatact tgactcaata ttttctccaa acatcgttcc
    45361 tgtagatgtt ggctttccat cattgtcaag atttacgagg actatgccac tggtggtgcc
    45421 aattaaaagg ctatcattat acagagccat ggattttgca ccggaaactt caatacttga
    45481 agtctcggtg ccatacttaa tcttgtttcc gtccatggtg tagacccctt ttgtcccgct
    45541 tgcaatgaga actgtatttg aaaaaacagt ttctgttcca tcatggacag ctgctacaga
    45601 ttttttatct ccatcgatta cttccgtggt ggtggtatga cttaataaat atagttttcc
    45661 atcatctcca gaagtagtta cataggct
//
LOCUS       DAOSSW010000005        42603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_1012_length_42603_cov_6.757873, whole genome shotgun sequence.
ACCESSION   DAOSSW010000005 DAOSSW010000000
VERSION     DAOSSW010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 42603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42603
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1012_length_42603_cov_6.757873"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..376
                     /locus_tag="PKW26_00930"
     CDS             134..376
                     /locus_tag="PKW26_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOQ92163.1"
                     /translation="MTITQMLGQSATLTLLGMGVVFAFLIILIIAMNLVKWFVIALKL
                     DKEEQSGSQASAGAVQQSTDQKSVVAAIAAALKLKK"
     gene            390..2438
                     /gene="oadA"
                     /locus_tag="PKW26_00935"
     CDS             390..2438
                     /gene="oadA"
                     /locus_tag="PKW26_00935"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HOQ92164.1"
                     /translation="MAHKVKISDLVLRDAHQSLHATRMTTDDMLPILGKLDKIGYESL
                     EAWGGATFDSCIRFLNEDPWLRLRSMKEKLPNTPIMMLLRGQNLLGYRHYADDVVDKF
                     VEAAAKNGVGVFRIFDALNDPRNLKRAADAAKKTGKHVQMAISYATTPYHTKEVYADL
                     AKTYADFGADSICIKDMSGLLKPYEVYDLVKAIKAKVDIPLQIHSHATTGMSVATLTK
                     AAEAGADVLDTVISSMAMGTSHSPTETIVEILKDTEMDTGLDIKALLEIAAYFREIRT
                     KYEKFESSFLGADTRILVSQVPGGMLSNLESQLKEQGAGDKIDEVLKEIPIVQKDCGY
                     IPLVTPTSQIVGTQAVFNVLFGRYNKLTAETRDLLTGRYGKTPAACNADLVKQALSEN
                     NMSAVVTERPADLIPNEWDKLVGEAKENGSDGSVEDTLTYAMFPKVAPKFFSERANGP
                     VKSDSFVVAKASTSPTSGPVSVSVNGTPYTVTTSPSGDVITVNGLAYNVAFGAATAAP
                     VATANTGVSSGADITAPVAGTLLKHVVPAGTTVKKGDTVIMIESMKMELEVKATQDGP
                     VSYSVNPGSAITAGQVLGAIGGGAPLAAPVQEAAPVQASPVAAAPSGGTVIPAPVAGT
                     YLKATVADGSQVSPGQTIIMIESMKMELEVKATVAGSVHFLVNAGTAINAGQALAEIK
                     "
     gene            2454..3836
                     /locus_tag="PKW26_00940"
     CDS             2454..3836
                     /locus_tag="PKW26_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOQ92165.1"
                     /translation="MLGQKKDTKKIFSVTLLLFGIAIVFSLMTTVFASDNKEEVASFR
                     TMNTAIIKESENVPEISLSKFLTNTVENTGIAAIVSGSEEVEDLVDASQTTTIPGWQR
                     LVLMAIGFLIIYLGAAKGFEPLLLIPIGFGTVFVNIPFGGMYAEHTGMLRIIYEAGVG
                     NEFFPMLIFMGIGAMTDFGPLIANPKTALLGGAAQLGVFVTLFGVALMNLVPGVDYNM
                     LEACAIAIIGGADGPTSIYVSGKLAPHMMAVIAVAAYSYMALVPIIQPPIMKMMTTKK
                     EREIKMKQLRHVSNVEKTIFPIALLVLSIAFIPTAAPLIGMLAFGNFVRQCGVVDRLS
                     KTMENELLNIVSILLSLGVGSQMTPDKILNPNAVGIIVLGLVAFMFATFGGLLMAKIM
                     NLFLKEKINPLIGSAGVSAVPMAARVSNKVGLEYDPSNFLLMHAMGPNVAGVIGTAIA
                     AGVFIATYGG"
     gene            3948..4928
                     /locus_tag="PKW26_00945"
     CDS             3948..4928
                     /locus_tag="PKW26_00945"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HOQ92166.1"
                     /translation="MIQKKLRFLTLIFASLIFLSCTSIQKTAMNSLASNLSGADKKGK
                     ALNKKNNENLMLPLTGETDSILMADFFPTALKLYEILHAQNPEHQGLSLMTASLSVMY
                     ANAFIQHKAEMLPQEAYLLQNAEFQRAKMHYLRARNLVFSVFEQRYPGFSKALLGFDI
                     DAIENYVARLKQSDVEAAYWLGAAWLGAWSLDPLDTSLLPTVHSAVRLLEKAALLEPD
                     YNEGAIWTILLSFYASAPADFGGDTERAYYAYEKALEASSGKSPGVYIAYAESICVAA
                     QDAEGFIDALQKALAIDANENPATRLQTIIAQEKANYLMDTIDYYFLFWD"
     gene            4941..5978
                     /gene="dctP"
                     /locus_tag="PKW26_00950"
     CDS             4941..5978
                     /gene="dctP"
                     /locus_tag="PKW26_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOQ92167.1"
                     /translation="MKKSTFVFILFCLLSLSCFAQKKITLKIASVAPARSAWETEQKA
                     LAAEWSRITNGQVVLQFYNTIALGGEDGVVQKMRPLRPGQPAPLDGAIFTNIGAYELA
                     PESNILTLAMPFLFKNQEEVDLVLEALSSEISDAMKVQGFELLGWFNVGWANFYTKKE
                     IRTPEELKKIKLSVGGVTSSELGKAFQRAGYMTEDVPNDKILQSIKSANGVQGLYTIP
                     MYAYASQYHKSLNYVLNTAICPVLANFVVSEASWNKIPNQYKDDLLAAVKQSEEKFIG
                     FQQKTDKEYLSLMEKEGLHIVNLTEKEIAVFQETLKMDALLMSESKTETIINYEMYQK
                     ISAILKKHREK"
     gene            5980..7836
                     /locus_tag="PKW26_00955"
     CDS             5980..7836
                     /locus_tag="PKW26_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOQ92168.1"
                     /translation="MNKKKIGLRLFVRNFENYFSLFIVAFLIILPLLFKIMNRFHFQI
                     LNSENAIVNLVFAFACFSGIITWREGNHISLASLTDGLPKRFKPFILSFRSATTVTIL
                     TALFLASFSASFTAFLPSQKVWGIPYLVFFALLPLAYFTMLLRNLFQKEYRLQNLLGF
                     FIGVLISLGPLSGVLYSLFKTESLGFLYRLNDYWLTFSAHAFIPLTLILVVFAFIGVP
                     LFVILGGIAYLAFSQGGGYVEVLSFEAYAVLTDKAIAAIPLFTIAGFILSKGSTGKRL
                     VSVFKSLFGWFKGGIVIAAVLVVTFFTIFTGASGVTILALGPLLVIALGGSGYKKEDS
                     EALITASGAIGLLFPPSIAIIMFGSVNYFSVDVYDIFKGAFLPGLILAISMILLGLSR
                     DVQKERDAFNGREILSALKSSVFEILLPFFVIFGFLGGFFSIIEAASFTLLYAFVLET
                     FVRKTFSLRESLVVITEAIPITGGILFILASARGLSYFMIDANIPELITSFITSHIHS
                     KILFLLLVNVFLLLAGCLMDIYSAIFIVSPLLMPICESFGIHPVHTAVIFLTNLQLGF
                     LTPPIGMDLFVASYAFDSPVMKIVKNVLPFILVQAVVLLLITYIPWFTTVFL"
     assembly_gap    7872..7971
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7972..9042
                     /locus_tag="PKW26_00960"
     CDS             <7972..9042
                     /locus_tag="PKW26_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOQ92169.1"
                     /translation="ELFCPGGFIFFSYNIGKTAETLMGFTDSILDYYKRDIPPFLSLD
                     LEGGEVNRLRNIISPLPSAEEISQLFTAEEAALFYKEIAKQSHLLGISLNLAPIVEPL
                     LEENAAFLKTRSFGSIEKTKFFSLAFIESFNSEGVLCTLKHFPGNTDVDPHLNLPVLA
                     LNKEDFEKNMLAPFRFVLENQKNNNAVLLSHALIPFISDLPASLSSEVVDLLVKDLDY
                     SGLIITDDIFMAALQSFFSEEKDAILLALDAGAHILLSSKKVFTQFLPFLEEIYTTET
                     VYKEKIDIALQRIIRAKIEAGLLGFSERESWSSLCLLKGNTSTSIDKIDQIDRKERER
                     LFYEAKKNASEILSEAYQNNFF"
     gene            9052..9879
                     /locus_tag="PKW26_00965"
     CDS             9052..9879
                     /locus_tag="PKW26_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOQ92170.1"
                     /translation="MAKKNKAKIDFDAYYTEIFGERWAVLKKNLSENPYSVKIQFHES
                     LSPYYMDAGSLFAASALPLDKAKTVLDLCAAPGGKSLVLAASLENTSSLIANELSMQR
                     RERLLSVLQQSLPENILEKVRVTPYDGRLMHKRNKEQFDAILCDLPCSSERHVLASPK
                     HFQEWSTGRIKNLSYTQGALLSSAFLMLKPEGFLLYATCALTKMENDDVIEKLLKKNA
                     NAAVLDIEIEELKGKIQKNYKLSLEIEPEKTEYGYHILPDSSNGAGPLYVSLIQKVL"
     gene            9929..11155
                     /locus_tag="PKW26_00970"
     CDS             9929..11155
                     /locus_tag="PKW26_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ92171.1"
                     /translation="MKRSTKEEKLQSIIQSEKFLNQTQDLDVLLETLLTEARTIVNAD
                     AGSIYVVEEDRLRIKYAQNNTQLKKLSAGEKLPFVSFSFPMNEYSIAGYVASKKEMLN
                     IADVYNIAADSTFKFNTQPDKDTGYRTKSMLTIPLVAASGKTLGVLQIINAQDQNANT
                     IAFDENAELYLNHFAANATIALERASLTRAMILRTIKMAEFRDPKETGAHVNRVASFA
                     VELYDRWAFEKEVPFEIQHKYRDTLRIAAMLHDVGKVGISDSILKKPARFTPEEHDII
                     KGHAVIGAALFNDIDTDIENMARDVALRHHERWDGTGYPGKIPPEVLENPFTPYQIRQ
                     GLSGEEIPLSARIVALADVFDALSSKRVYKDSWKEEDVYEEIRKSSGTHFDPDLVTMF
                     FAILPRIKEIQAMWPD"
     gene            complement(11152..11622)
                     /locus_tag="PKW26_00975"
     CDS             complement(11152..11622)
                     /locus_tag="PKW26_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HOQ92172.1"
                     /translation="MFSLWVDADSCPVEIRKLLIRFSNRLEIPLYYVANRMIPHEEAK
                     YFKMIITSTDEGSADDYIVENASPEDLVITRDIPLAARLVSKGLTCLNDRGDIFTEEN
                     IGEKLALRNLNLDLFMQGLKAESHTQLGKKEMNRFANALDRLIQKKLKEIKTDF"
     gene            complement(11622..11912)
                     /locus_tag="PKW26_00980"
     CDS             complement(11622..11912)
                     /locus_tag="PKW26_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOQ92173.1"
                     /translation="MGTLQINILGTSFAIQADESNDYLETLLAYYSQMVKQVENVAGA
                     DALKTAILAGIMLCDELYKEKISGKQKLPSDELNELERITLSLIQKIDTAIK"
     gene            complement(11921..12397)
                     /locus_tag="PKW26_00985"
     CDS             complement(11921..12397)
                     /locus_tag="PKW26_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HOQ92174.1"
                     /translation="MITLGQIHLLEEKIEEVLKRLQSLEEENKMLLDKNNALKKEKEN
                     LAYRISTFEADQEQIEKGILNALDKLNTIESAVIKESISHTVKSQEVEISTANQVIDE
                     VQAPAEQELHGSQTETKHEEAINEPSTEIDDDFSLEIEEEIEIPSSKNETTEFDIF"
     gene            complement(12401..12760)
                     /gene="rplT"
                     /locus_tag="PKW26_00990"
     CDS             complement(12401..12760)
                     /gene="rplT"
                     /locus_tag="PKW26_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOQ92175.1"
                     /translation="MSRVKGGTKHNTKRKKILGLAKGFRGRRGTNFKAAKDAVMQALT
                     DSYVGRKDKKGDMRSLWISRLNAAVRDQGLSYSRFMNGLTKAGVIINRKALSNMAIED
                     PVAFEAVINTAKKAIGA"
     gene            complement(12837..13037)
                     /gene="rpmI"
                     /locus_tag="PKW26_00995"
     CDS             complement(12837..13037)
                     /gene="rpmI"
                     /locus_tag="PKW26_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOQ92176.1"
                     /translation="MPKMKTKKAAAKRLSLTGTGKVKYKKMNLRHILTKKAPKRKRQL
                     RSAGILSPADSAKVRKQLLPYG"
     gene            complement(13051..13572)
                     /gene="infC"
                     /locus_tag="PKW26_01000"
     CDS             complement(13051..13572)
                     /gene="infC"
                     /locus_tag="PKW26_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOQ92177.1"
                     /translation="MADNKGLRVNEQIRVREVRLIDDEGEQKGIVPTLEALKLAREKE
                     LDLVEIAPTANPPVCKILDYGKYRFEMEKKLRDSKKKQKLLKLKEIRMQPKIGSGDLD
                     FKSKHVKEFLAEGNKVKVTVRFRGRELAHTELGLVVLDEVLKRLDEEYVLEKPAAMEG
                     RFMSMTLAPKPKK"
     gene            13750..14382
                     /locus_tag="PKW26_01005"
     CDS             13750..14382
                     /locus_tag="PKW26_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92178.1"
                     /translation="MWLFSFFLIPILFSFDFFQPEKENQKSYLRYFIGLLLAIGYSVL
                     IMLIYSSREYTPAKLWYLSYRNLLLFVLPLVIVFILYFLVSVKSFFGKVLSLVDLFAG
                     FYTIFIPFYVLSYFATQTFFSLFVFPILFTASLFLLSYSLSAFIKLINTRGSLLSFIL
                     TAFFILIALVLPSLLLALWTLGISTILLSIFVLLYLTSAGVLIFLYKKIW"
     gene            complement(14449..15450)
                     /locus_tag="PKW26_01010"
     CDS             complement(14449..15450)
                     /locus_tag="PKW26_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOQ92179.1"
                     /translation="MVSYKDLGLVNTKEMFAKAMKGGYAIPAYNFNNMEQMQAIIQAC
                     VETKSPVILQVSSGARKYANQTLLRYMAQGAVEYAKELGCKNPEIALHLDHGDTFELC
                     KSCIDTGFSSVMIDGSHLPYEENVALTKKVVEYAHQFGVTVEGELGVLAGVEDDVSHE
                     VSHYTKPEEVEDFVKRTACDSLAISIGTSHGRQKFKPEQCTVNADGVLIPPPLAFDIL
                     EEIEKRIPGFPIVLHGSSSVPVEYVKTIEKFGGKLSDSVGIPEEQLRKAAKSAVCKIN
                     IDSDGRLAMTAAVRKVFAEHPEEFDPRKYLGPARDELKKLYMHKNKEVLGSAGAIAN"
     gene            15620..16351
                     /locus_tag="PKW26_01015"
     CDS             15620..16351
                     /locus_tag="PKW26_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOQ92180.1"
                     /translation="MKQGVMVLLCLFCVFLFCVPVFAQPSTKSVETVLIDDFDSAEEK
                     DYQWDVQASRFVADGFPKKGYFKGIPNSLRGFYGDGGTEALVLGVQTSFNRKGDNWFE
                     IFPTITDENGEIANYEIPFKGVVSQIDFWVWGSNYKYILEVLVRDADGRVHTLPAGVL
                     LFNGWKNFVVNIPGHIRQESRLRSGPKNMTFVGFKVRTDPNEYVDNFNIYFDNLKYTT
                     HTLSTIYDGFDLRDIDFDSANEEGK"
     gene            16351..17079
                     /locus_tag="PKW26_01020"
     CDS             16351..17079
                     /locus_tag="PKW26_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOQ92181.1"
                     /translation="MKKIVLLVCCLLSLGLVVAQQNGNTSLSDPDPTMIGADSAKQSL
                     REVSVDLFEREGAWNAKISPDYGVISARLFEGSPAGKEVLDDVENQQISDDRVLGVKV
                     EFFRRGINSFFITAARPIAIEGTTKTISVWVVGRNQSHNLTVLVQDYFGNNFELYMGQ
                     LNFSGWKKLTVAVPPSPDGVHGIVQTSAYHGDRPGLRIVGFRVDCNPELARGSYYLYL
                     DDLRAVTDLYAMENRDEDDMDDNW"
     gene            complement(17165..17869)
                     /locus_tag="PKW26_01025"
     CDS             complement(17165..17869)
                     /locus_tag="PKW26_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92182.1"
                     /translation="MYSIEMIQTYNESSLHKSLKKIYKKKYKAEEEVKIEGFICDLLT
                     SNNEIIEIQTRHLSALNKKILKLSKKGYSFRIVHPLITKSIIKTIDSSACLLSERKSP
                     KKADIYSLFSELLGFYQSFAVENWVLEVLFVEQVEIREKQAKKTQNKTNTRRYLKDWI
                     SLDKQLLGINDHLHIKNKEDLVQLMPELPALFCAKDLSKTAIKKNAHKVLWVLHKLDL
                     IRLVEKKGNTKYYQYI"
     gene            17999..18442
                     /gene="mraZ"
                     /locus_tag="PKW26_01030"
     CDS             17999..18442
                     /gene="mraZ"
                     /locus_tag="PKW26_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOQ92183.1"
                     /translation="MNLITGEYRNTLDEKGRILFPSRLRSELKETHLIITQALDGCLW
                     LFTPDEWRGFSSKLMESASPFNSNSRSVLRRLVAPAQEIEFDKSGRLSIPQSLREYAG
                     LEKDCVILGINKFIELWDAEKYKAYLDKTQGSFDEATEELSQIFF"
     gene            18454..19398
                     /gene="rsmH"
                     /locus_tag="PKW26_01035"
     CDS             18454..19398
                     /gene="rsmH"
                     /locus_tag="PKW26_01035"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002678681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOQ92184.1"
                     /translation="MEAVHTSVLLQEVLDYLDPRHGENPESPFLIDSTLGEGGHSFAF
                     LKAFPQLQVLGLDADAEIQKRAKERLKEFGPRMRFFRGWFNDFYRNYPEELAKPDRIL
                     FDLGISIFHYELSEMGFSFNKDERLDMRLNHDEKISAYEIVNESLEEELASILFFNAD
                     ERYSRRIARAIVTEREKAPVESTIALRDIVYRAVPVAYRHGFTHPATKTFQAIRVAVN
                     HEFDRIKQALEDAFMQLKPGGRMAVISFHSIEDRIVKHTFRDLGKSCVCPPDMPICKC
                     GGSPRAKILTRKPIEPTSEEIEKNPPSRSAKLRVIEKI"
     gene            19424..19717
                     /locus_tag="PKW26_01040"
     CDS             19424..19717
                     /locus_tag="PKW26_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOQ92185.1"
                     /translation="MVLKKEKKVSRVSIYFIALLIPSFLCLDSMQSLKYVSIQREIRE
                     LEKTEAKLIEKNKTLIADISNLSSTSRIEAIAVNELAMRKATSSEIMRVEVGR"
     gene            19714..21201
                     /gene="murF"
                     /locus_tag="PKW26_01045"
     CDS             19714..21201
                     /gene="murF"
                     /locus_tag="PKW26_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOQ92186.1"
                     /translation="MRKMNENTLTNLLSLSEVLLATEGKLLNPTYSEEDFVFTSITID
                     SRNCVEGSFFVPLVGNLDGHDFILSAAENGAKTVFVSDFYAKFHEKEIASFIDSGLCL
                     ISVSHTMYALQKMAAYYVEKFPNLKKVAITGSSGKTTTKEILVSLLQKKYKTVYNEGN
                     LNSETGLPLSVFAIRDEHEWAVFEMGMNRRGEIRELAQVLKPNIAIITNIGSAHIGNL
                     GTKDLIAEEKKSVFSFFETSSIAIIPVFDEYSEYLQTNTKGQIVLYGNYEALHIRAVK
                     DRGLEGSVFLMEDQEVSFPLYGSFNFLNALAALHCAKIAGLGINEIKRGLEELRPSFG
                     RAEIFNGEIAIVQDCYNANPESMNASIEYFETLHWEGRKILILADMLELGFDSETQHR
                     LVGKKLKSLSFDMIFLLGSQMDWAYEELMKGQSTGENTDEKNEKKIYYNAFISDADFG
                     TAVNDILVHVNKGDILLLKGSRGMKLERISEALVKAFNMQGCTIC"
     gene            21195..22340
                     /gene="ftsW"
                     /locus_tag="PKW26_01050"
     CDS             21195..22340
                     /gene="ftsW"
                     /locus_tag="PKW26_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOQ92187.1"
                     /translation="MLEMNVVPEKAVEKNTSDMYFIATMLLLLGLGLVTLFTCSASYA
                     NRIFDDSFYFIKRQLISCFIGFIVLFILSYIDMQFVRKMLPLIVIGTFFLCLLTYLPG
                     IGVERNGARRWIRLPFISNFQPSELVKIAIVLYLANLFDKKYEIINDVKKTIIPAVSG
                     IMLFVFVIFLQDDFSTAFFILLVSVSMFFLAGLSLYWFFSLFLVSIPVLILFIFTESY
                     RVNRILAFLNPLQDLHGINYQINASIKAISNGGFWGEGFGIGLQKSSAIPEVQADFIF
                     AGWVEGMGLFGVLLYLLLLGFFAWRIYLIAFRAEDRFSSYVAFGLGTSLVLQSLLNIA
                     VVVGAVPATGIPLPFFSSGGSSLLISMAMCGLIINISKNNKKEAKYE"
     gene            22333..23148
                     /locus_tag="PKW26_01055"
     CDS             22333..23148
                     /locus_tag="PKW26_01055"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOQ92188.1"
                     /translation="MSDGLIFDNSESFCSIEKKEKNQKTKLRILKILFFVLLSFVLIE
                     LILYSLVIPVFSNIQFSVTGLETLSEKEIAQYIQLSSNDTWFNFNTSRISQKLASLSQ
                     VETVSVEKRFPDRVAIHITERKAVAFSLATIQGKTVPVQIDKKGVVFAIGKELGNTAI
                     PLITGLALDNISEGARLHAKLLPLIEQICEIQIINSDYFNVLSEIRVLPKDYGAYELA
                     LYPLNTHIRFLSDSVLNVESLQYIMVSLDVFKKTNTMVKEVDLRNGVTSFKTL"
     gene            23176..24423
                     /gene="ftsA"
                     /locus_tag="PKW26_01060"
     CDS             23176..24423
                     /gene="ftsA"
                     /locus_tag="PKW26_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOQ92189.1"
                     /translation="MSDIIVGLDIGTSNVRVVIAELNELGKLQVIGVGKSLSTGLRSG
                     LVVNIDATMKAIEKAVEDAEMMAGCEVKSVFISLGGSQIESQNSKGFVAVAQKAKDSR
                     EITEDDIARVIQASQAIAIPLDREILHVIPQEYIIDGSQRTKEPLNMIGVRLESEVHI
                     ITASVSPMQNIIKCVKRAGYDIDGIMLKTLAATESVMTEEEKDLGSILIDLGGGTTDV
                     LVVLGGAPICTATIPIGGIFVTSDISTVRGVSIETAETIKLSDGCCWEGLLSDDEYFL
                     IPGVGGRPPEQVSRKELCEIMQPRVEEILKMVKEKVYQLTKLKKLSGNVVLTGAGALM
                     PGIIELAQDVFDTASVRIGTSANLGNVTDAYRSADFATATGLLVAFKDRLEVKAQGPG
                     ASLPVAEKNSILKSIGSFFKEFF"
     gene            24449..25645
                     /gene="ftsZ"
                     /locus_tag="PKW26_01065"
     CDS             24449..25645
                     /gene="ftsZ"
                     /locus_tag="PKW26_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOQ92190.1"
                     /translation="MIEFEMLEQDYKLNPTKIKVIGVGGGGSNAVNRMIAAGLKNIDF
                     IAANTDLQALSTSRAQTKIGIGSKITGGLGAGGKPEVGEKAAVEDTDEIANLVKGANM
                     VFVTAGMGGGTGTGAAPIVAKIAKESGALTVAVVTTPFQFEGAFKMRLAQEGIEKLKP
                     NVDTLIVIPNENLKKVNEKNLTMAKMFMIADDILRQGVQGISDLITQHGETNINIDFA
                     DVETTMKGKGDAIFGVGVSRGENRAVDAATQSINNPLLQDSYIDGAENILLNISSDES
                     LAYDEVEEIVNIIRSSADPNVHLIYGQTFDANMEDSIKVTVIATGFAKDRSMKKQLDS
                     SMFSKQQTKKDIDKDSYISSDMWSEIQGEEKKEKNQAQYLSSRNQNDLETPAFYRNKS
                     GINLKP"
     gene            25656..26534
                     /locus_tag="PKW26_01070"
     CDS             25656..26534
                     /locus_tag="PKW26_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOQ92191.1"
                     /translation="MVHAFEDELLSVDRRSRLSVETYGFSAQAFLDWCDTKEIALEKV
                     GLENLIAYGIERKKAGIDGATIAKDIAALRAFGAFLVRKNIWKENYALLLEKPKLHRS
                     LPRVLELEQVEAILDAIDTTKPLGVRDRCLFEIIYSSGLRISEASSLLMQNLQLKEQI
                     LWVQGKGDKERILPFGDEALYWFQKWIHEERPKILKQKQSPYVFINYAGGQLSRKGIW
                     KKFQDLKAKTGLEAKVHTLRHSFATHLLQGGADLRTVQELLGHQDLATTQIYTHIDAS
                     ELQKYHERYFKKKSDK"
     gene            26617..27360
                     /locus_tag="PKW26_01075"
     CDS             26617..27360
                     /locus_tag="PKW26_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ92192.1"
                     /translation="MKKQKIFFKIARLILLMAFLSILLACGPSVKSVKRLQFMEEKVS
                     SPNTIEELEAAVAKFEKRVLDISEANSQIGIWYKMIATRYLDKQMYNEALKALEKAVL
                     YYPTNQNLYYFIGVSAGFMAKASLDFEANGSRVKAEYYLKLSESAYKRAIELEPTYVR
                     SLYGLSVLYLFDLNEAEKAIPLLQKVLSIETKHYDAMFLLARAYYMSYEFEKALDVYD
                     KIISLSASEQRTRQAEENKKIVLDALYGD"
     gene            27366..28331
                     /gene="dprA"
                     /locus_tag="PKW26_01080"
     CDS             27366..28331
                     /gene="dprA"
                     /locus_tag="PKW26_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOQ92193.1"
                     /translation="MRALALLLSHYDFFSLSEKYLILEQAKDIQTFLKMRPIEIASLI
                     GRDFKIDKLNFQSIEKRLEKTIALMRAYSIGLVFYDEEAYPPLLKEIHDPPFALFYRG
                     EASLLHSLCLGIVGTRRPTGRGMKASFDIAKSLAENALTIVSGLARGIDGYAHKGALE
                     AKDGKTIAVLASGLDSIYPSDHKRLALQMLEKGSLLLSEHPPLTPALTWHFPKRNRII
                     AGLSEGVLVVEAPRGSGSLITASCAIEENRELFFHELALQYSFQKLALSASDKQKLQK
                     KWAVQNYIDDGALLISSAKDIIQHLSEGSIKNTSLLFSQYKLDLN"
     gene            28363..30582
                     /gene="topA"
                     /locus_tag="PKW26_01085"
     CDS             28363..30582
                     /gene="topA"
                     /locus_tag="PKW26_01085"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOQ92194.1"
                     /translation="MAEKKTTTKKVATKKTTTKKTKQKQTLVIVESPAKAKTIEKYLG
                     PGFKVEASMGHLIDLPKSRLAINVEDKFQPEYITVRGRAKILKNLQKEAKKSETVLLA
                     SDNDREGEAIAWHLKNSLSEKTEASIKRIVFNEITPQAIKDSVENPAEINEYKVNAQK
                     ARRVLDRLVGYNLSPLLWKKVKNGLSAGRVQSVALKLICEREKEVEAFIPEEYWSLEA
                     EFSKGKTVFPAQLFQYKGEKPVLKSGQDVNDLIAEIESAQSFVSDIKESEKTIRPKAP
                     FTTSTLQQTAANRLGFTSRKTMQIAQQLYEGVNFGQDRIGLITYMRTDSTRISASALE
                     EVRTFIGELYPQELPEAAIEYAVGTKAQDAHEGIRPTYVSYTPDYVKEYLNRDQLRLY
                     TIIWERFVSSQMTNAKSRTTTVEISAGDAKFRASKSKLVEKGFYQVIKLLSTKEEQSG
                     KIPSVKLNEELEVEAFHPAQHFTQGPPRYTDASIVKALEEKGIGRPSTYAPIISVLLD
                     RYYVTRSNKQLVPTQLGRIISNILSDSFPNVVDENFTREMEEKLDNVEEDTQDWVTMI
                     ADFFHPFKDRVDEVMSNLESMKGIMDEPTDKICELCGKPMLKKLGRFGFFIACSGFPE
                     CRNTRSLPLAPCPKPDCGGEIVARKTKGRGKEFYGCTNYPECDFISHYKPINASCPEC
                     GYFLVEKYDKKNGSYKSCINPECDYLHSTSEAEEKKSEEIAQELAESQRALTGEAEE"
     gene            30585..31499
                     /locus_tag="PKW26_01090"
     CDS             30585..31499
                     /locus_tag="PKW26_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOQ92195.1"
                     /translation="MFLGEAIEAFLQYADGIKNLSPHSLKAYRNDLSFFLSSFPEKEK
                     KILFEISLIDMRSCVGLLSKQKKTATTINRFICTIRSFFLYLYRMNILPNNIALDLKT
                     VKIPKKLPRYMSDSEVDALRRQPEKKELLWTERDRAIFECLYSSGARVSELSNLNLRD
                     LAANLSSAIVTGKGSKDRKVFFSQDAVDALKVYLLQRAEIIQKNEVKGEALFINQRGG
                     RLTARGIFYVVNRYSSVEGTNKPVSPHAFRHTFATTMLKNGANIRVVQELLGHENIST
                     TQKYTHITKEQLIQTYNASHPHGGKNEL"
     gene            31489..32037
                     /gene="hslV"
                     /locus_tag="PKW26_01095"
     CDS             31489..32037
                     /gene="hslV"
                     /locus_tag="PKW26_01095"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOQ92196.1"
                     /translation="MSFEYEKIRSTTIIAVKKDGKMAMAGDGQVTMGNTVMKGNAKKV
                     RRMYDNTVLTGFAGATADAFNLFDKFEMKLKEYSGDITRAAVELAKIWRTDKALRQLE
                     ALLLVANKDKILLISGTGDVIEPENDVLAIGSGGNYAYAAALAYIESSNLSAKEIAEK
                     SLKIAGNICIYTNQNLIIEELE"
     gene            32048..33463
                     /gene="hslU"
                     /locus_tag="PKW26_01100"
     CDS             32048..33463
                     /gene="hslU"
                     /locus_tag="PKW26_01100"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOQ92197.1"
                     /translation="MSDKTELTPKQIVSALDTYIIGQQKAKKAVAIALRNRMRRLKLP
                     EDIREEVAPKNILMIGPTGVGKTEIARRLAKLTAAPFLKVEATKYTEVGYVGRDVESM
                     IRDLMAVGFNMVKAEMQEGLKEAAEQRVEEALLDLLLPPSPSKKKEQSNPPFLLNSAF
                     GSENAETVQSSQKTESEDASTRERFRQWLREGKLEDKEVEITITKQNRMPTMEIFAGG
                     NIEDFEETMQNITSMLSGGRSKKKMLSIKDARRILLEEELDKLVDTEKVSEEARKRVE
                     EMGIIFIDEIDKIAVSNSRGSQDVSREGVQRDILPIVEGSTVNTKHGIVDTTHILFIA
                     AGAFNLAKPSDLIPELQGRFPLRVELDSLTADDFKKILTEPKNALTKQYSELLKTESV
                     TIDFKAEAIDTMSAIAQEVNAKVENIGARRLHTIMETVLEELSFEADERSGETIEITS
                     RYVEDRLESIVKDQDLSKYIL"
     gene            33523..33936
                     /gene="flgB"
                     /locus_tag="PKW26_01105"
     CDS             33523..33936
                     /gene="flgB"
                     /locus_tag="PKW26_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOQ92198.1"
                     /translation="MNSFTRSVDLLHRAMDVSTLRYSVSANNLANAGVPNFKRTTVNF
                     EAALKEAIDSEKNAKDSFVLKTSDDRHIQSKTYTDYRTVEPRRVLDYATTSDANGNNV
                     DAEAEAMDILKTQLNYQLLTQMQSFQFSQMHIVLK"
     gene            33947..34402
                     /gene="flgC"
                     /locus_tag="PKW26_01110"
     CDS             33947..34402
                     /gene="flgC"
                     /locus_tag="PKW26_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOQ92199.1"
                     /translation="MGLFTSINIAATGMSVESLRRDIISDNIANASTTRTADGGKFKK
                     STLILEAIQDDLQYRNPFVPADKDSGVGKGVRALRVEKDNSQGRLVYDPTHPDAIQTG
                     PNTGYVEYPNVNIVNEMVNLISASRAYEANATVIQGSKEMFQSALQIGK"
     gene            34432..34782
                     /gene="fliE"
                     /locus_tag="PKW26_01115"
     CDS             34432..34782
                     /gene="fliE"
                     /locus_tag="PKW26_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOQ92200.1"
                     /translation="MISSLDLLRYNASTTASNAVSSTQLSKALEKTKDGLGITNTEEG
                     KSFDNYLLDAISYVNDSQNKSSALFEQVIIDPDSVDAHDVTIAMAEANLSLSLAQAVI
                     DRMIKGWSEITTTR"
     gene            34932..36638
                     /gene="fliF"
                     /locus_tag="PKW26_01120"
     CDS             34932..36638
                     /gene="fliF"
                     /locus_tag="PKW26_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOQ92201.1"
                     /translation="MNEWLKKLFSQIKTVWSKGSTVQKVVITGIIIFVIGAIVFISVL
                     SSSPSTVPLYNIPITDEDSRDRILIRLSEENIQATVNAAGIISVADEKTARKARTLLV
                     REDLTPTNVSPWNLFDTERWTTTDFERNVNLLRSITNQVTQHIESLEEVDRANVVITR
                     PERTLFAADQNPTTASVILYHRPGSDILTNKKKLQGIQKLLLFAVEGLKEENITITDS
                     NGNVLNDFAGMAEIDRVDIIAREQKLISTLETQYRAKVLSALQQIFTPDRVRDLNIKI
                     DMNMSKRFVDATEYSPITIRPDNPDTPYDDSELVESITLSSETVTKRWTGTGYNPEGP
                     AGAEGQTAPVYSDMSNLYGVSEETGVKRNEVINTKQIQEEISPSIDRISVSVNIDGRN
                     RIVYSDKGEPELLPDRSIKREYIPLTAEELANTTRLVEDAIGYQRGRGDSVSVQNIQF
                     DRTAQFAAEDAALLRAQQTKKTVFYILSGIVLVLLAFIIFRTISRELEKRRRLKEEEL
                     LRQHAKMRQETFLEAERAGMEVTMSVEERRRAELQENAIALAKDHPEDVAMLIRTWLM
                     EE"
     gene            36640..37719
                     /gene="fliG"
                     /locus_tag="PKW26_01125"
     CDS             36640..37719
                     /gene="fliG"
                     /locus_tag="PKW26_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOQ92202.1"
                     /translation="MAAQAKSKPSAPAGATASTGSKKPKDYKSLTGRQKAAIFLVSLG
                     SELSSEIFKHLREDEIETLTFEIVRLDTVDSEIKQAILEEFQELMAAQNFITSGGIDY
                     ARELLEKSLGSQKAIDIINRLTSSLQVRPFDFIRRTDPAHLLNFIQQEHPQTIALILA
                     YLEPNKASVILQNLPDEIQSDVARRIATMDRTSPDVLREVERVLEKKLSTLSSEDYTA
                     AGGVESIVEILNLVDRSSEKSIIESLEEDDPDLAEEIKKRMFVFEDIVMLDDRAIQKV
                     MREVDTQELAKALKAVDTEVQDKIFRNMSKRAAAMLKEDMEFMGPVRLKDVEEAQQKI
                     VSTIRRLEDSGEIVIARSGEDEIVV"
     gene            37779..38714
                     /gene="fliH"
                     /locus_tag="PKW26_01130"
     CDS             37779..38714
                     /gene="fliH"
                     /locus_tag="PKW26_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HOQ92203.1"
                     /translation="MAKAVFRPTEITNTDSKVILKLSHNYAPEEEEEEISDIPEYLGP
                     TADDLRREAEAFKKEWEAEKEALLAKTDEEAEKIIKNAENAAFQEVKRKTDEANIIKA
                     EAELEAQKIIQEAEQKALKIQEEAEKNRDSLVKDAHKEGFDKGSEEGYRSGQNEVDRL
                     IGRLHLIIERIMSKRQEILDETEQQIVELVILMTRKVVKIISESQKNVVMSNIVQALR
                     KVKGRGDVTLRVSLEDLQLTSDHTKEFMKAVENIKNITVVEDTTIEKGGCVVETDFGA
                     IDARISSQLNELEQKILEISPIKTITKSSSINPEL"
     gene            38718..40073
                     /locus_tag="PKW26_01135"
     CDS             38718..40073
                     /locus_tag="PKW26_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HOQ92204.1"
                     /translation="MKTLIDKYIRTADTTDTILYTGSVISVKGMLIESHGPQSVMGEI
                     CNILVSVPGEKTSRSILAEVVGLHHTTVQLMAYEETKGIEIGCKVIASGSVLQVGVGE
                     ALLGRVIDARGKACDNKGEIYTTSFYPALASAPPALSRKPITERIVTGVRAIDSLLAV
                     GKGQRLGIFAGSGVGKSTLLSMIARNSNADINVIALIGERGREVLDFIHRDLGEEGLK
                     RSVVISATSDQSPIARLRGAYVATAVAEYFRDQGKDVMLMFDSVTRFAHAQREIGLSA
                     GEPPAQRGYPPSVFELLPKLLERSGTSEKGTITAFYTVLVDGDDMDEPITDKVRGTLD
                     GHIVLSRRLAQAYHYPAIDVLASISRLANRVSGPKTKEACGVLRRLLAIYAESEDLIS
                     VGAYQKGTNPDIDRAIEKHADIEAFLMQEENEPSTIQETIKRLGEIAEIAIPKEELET
                     L"
     gene            40070..40489
                     /gene="fliJ"
                     /locus_tag="PKW26_01140"
     CDS             40070..40489
                     /gene="fliJ"
                     /locus_tag="PKW26_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOQ92205.1"
                     /translation="MKKFNFRLEKILSLRDFAEKEAKIELGRAISHAQTIQNHIEALA
                     KEKHEQNKARSKTSAIYDLQAIEAYINRLDLKKEELFAELAKAEIVVEEKRALLQEAM
                     KERKVLTKLKEKKQESHRKEVLLDTEKILDDIANRQI"
     gene            complement(40486..>40994)
                     /locus_tag="PKW26_01145"
     CDS             complement(40486..>40994)
                     /locus_tag="PKW26_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOQ92206.1"
                     /translation="QMQLCKEDILILEGIHGLNDRLTPLLPNDIKFKIYLSALTQLNL
                     DDHNRISTSDNRLIRRIVRDSQFRGKTAVDTIKMWPSVQRGEKLHIYPFQGNADAMLN
                     TALDYELAVLKMYAEPLLRAVNPNQVEYSEASRLLRFLSNFLPIPSNLVPGQSLIREF
                     IGGSDFKY"
     assembly_gap    40995..41094
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<41095..41969)
                     /locus_tag="PKW26_01150"
     CDS             complement(<41095..41969)
                     /locus_tag="PKW26_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOQ92207.1"
                     /translation="MKEIKISIDGKKEIKVSKRICAYDILSEFEENDDEIIAVKINNE
                     TCSLTQVIDIHCTLKPIYLKTHEGSAIYRRTLCFVLAAAAQELFPAKRLLVGHSLGYG
                     YYYTFDSDFVLQQKDLENLEKHMRKLIYADHIIKQSYISYNDAIELFEKLNLTETRKQ
                     LNYICPPKVPINTLGDFCDLYFAPLLVSTGILKLFELKLYQGGFLLRFPMTAKPDEIS
                     DFEDLPQLFSVYNRYKTWGKQLNVSSCASLNALIHERKQKEFIDIVETFQNKTIADIA
                     DQIHEREGIRVILIAG"
     gene            42085..>42603
                     /locus_tag="PKW26_01155"
     CDS             42085..>42603
                     /locus_tag="PKW26_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ92208.1"
                     /translation="MKIEIRNPQAADIAYLYDICLKTASNGQDAADLYTDPWLVGQYY
                     VAPYVFFDPSNCFIAYDSESEYKRPLGYIVGTDDTRAFYDWFYSEWAPQLRKVYGKKM
                     PPQSKYEAWLINEIYNKNKQVDDVLLDSYAGHLHIDLLPVLQGQGVGRSLIDRFSNAL
                     RKKMSNGIHLGVS"
BASE COUNT        13184 a         7594 c         8529 g        13096 t
ORIGIN      
        1 attatacacc cattgttcca aatttgcact actggcttga ataagattgt caaaatttat
       61 ataattgtca tattatgctt ttattttact atgatagtaa tcaatagata gtaatcaatt
      121 ttttggggga attatgacaa ttacacaaat gctcggacaa agtgctacat taactttgct
      181 cggaatgggt gttgtttttg ccttcttaat cattttaatt attgcaatga atcttgttaa
      241 atggttcgtt atagcattaa aacttgacaa agaagaacaa tctggatcac aagcatctgc
      301 tggtgcagtt caacaatcaa ctgatcaaaa atcagtcgtt gcggctattg ctgcggcatt
      361 aaaattaaag aaatagataa ggggtttcaa tggcacataa ggtaaaaata tctgacttag
      421 tattacgtga tgcacatcaa tctttacatg caactcgcat gacaacagat gatatgcttc
      481 ctattctcgg aaagctcgat aaaatcggct atgaaagtct tgaggcatgg ggaggggcaa
      541 ccttcgactc atgtattcgt tttttaaatg aggatccatg gctaagactt cgcagtatga
      601 aggaaaaact tccaaatact cctataatga tgcttcttcg aggtcaaaat ctccttggct
      661 atagacacta tgcagatgat gttgttgaca aatttgtcga agcagctgca aaaaatggcg
      721 ttggtgtatt tcgtattttt gatgcactga acgatccacg aaacttaaaa cgtgctgcag
      781 atgctgcaaa aaaaacaggc aagcatgtac aaatggctat ttcttatgca accactccgt
      841 atcatacaaa agaagtctat gcagatttag caaaaaccta tgctgatttt ggtgcagatt
      901 ctatctgtat taaagatatg tcagggcttt taaaaccata tgaagtgtat gatcttgtaa
      961 aagcaataaa agcaaaggta gatattccac tacaaattca ctcacatgct acaacaggta
     1021 tgtctgttgc aaccttaaca aaggctgctg aagcaggtgc tgatgttctt gacactgtta
     1081 tttcatctat ggcgatggga acttctcata gtccaaccga gaccattgtt gaaattctta
     1141 aagacaccga aatggatacc ggtcttgata tcaaggcgct tttagaaatt gcagcttatt
     1201 tccgtgaaat ccgcacgaag tatgaaaaat ttgaatccag tttcttaggt gctgacacac
     1261 gaatcctcgt ttcacaagtt cctggtggaa tgctttcaaa cctcgaaagc caacttaaag
     1321 aacaaggtgc aggagataaa atcgacgaag ttcttaaaga aattccaatt gtacaaaaag
     1381 actgtggtta tattccgctt gttacaccaa cgagccaaat tgttggaaca caggctgtgt
     1441 ttaatgtgct tttcggtcgc tataacaagc ttaccgctga aacacgcgat ctattaactg
     1501 gtcgttatgg taaaactcct gctgcttgca atgcagatct tgtcaaacaa gcactttcag
     1561 aaaataatat gagcgctgtt gttacagaac gtcctgctga ccttattcca aatgaatggg
     1621 ataaattggt aggtgaagca aaagaaaacg gttctgatgg ttctgtagaa gataccttaa
     1681 cctatgcaat gttcccaaaa gttgcaccta aattctttag cgaaagagca aatggtcctg
     1741 taaaatctga ctcctttgtt gttgcaaaag caagtacttc tcctacaagt ggtcccgttt
     1801 ctgtttcagt taatggtact ccatatactg ttacaacaag tccttctgga gatgttatta
     1861 cggtaaacgg tcttgcatat aacgtagctt ttggtgcagc aactgctgct cctgttgcga
     1921 cagcaaatac tggagtttca agtggtgcag atattaccgc tcctgttgca ggaactcttt
     1981 taaaacacgt agtccccgca ggaactactg taaaaaaggg cgacacagtt atcatgattg
     2041 aatcaatgaa aatggaactc gaagtaaaag ctactcaaga tggtcctgtt tcatattcag
     2101 taaatcctgg ttctgcaatt actgcaggcc aagtgcttgg cgctatcggt ggtggtgcac
     2161 ctcttgcagc tcctgtgcaa gaagcggcac cagttcaagc gagtcctgta gctgcagccc
     2221 caagtggagg cactgttatt cctgctcctg ttgcgggaac ctatctcaag gcaactgttg
     2281 cggatggttc acaagtaagc cctggacaaa caattataat gatagaatct atgaaaatgg
     2341 agcttgaagt aaaagctact gttgctggtt ctgttcattt cttagtaaat gctggtactg
     2401 ctataaatgc agggcaagcg ctcgcagaaa ttaaataagg ataggtatat aaaatgcttg
     2461 gtcaaaaaaa agacacaaaa aaaatatttt ctgtaacttt attgctcttt ggaattgcta
     2521 ttgttttttc attgatgaca acagtttttg cttcggataa caaagaagaa gttgcatctt
     2581 ttagaacaat gaacactgcg attatcaaag aaagtgaaaa tgttccagaa atttctcttt
     2641 caaaattttt aacaaacact gttgaaaaca caggtattgc agctatagta agcgggagcg
     2701 aagaagttga agacttagtt gatgcaagtc aaacaacgac tattcctggt tggcaacgtc
     2761 ttgttcttat ggcaattggt tttttaatca tttacttagg agcagcaaaa ggctttgaac
     2821 cacttcttct cataccaatt ggatttggaa ctgtttttgt aaacattcct tttggtggaa
     2881 tgtatgcaga acacactgga atgctacgaa tcatctatga agctggtgtt ggaaacgagt
     2941 ttttccccat gctcatcttt atgggtattg gtgcaatgac agactttggt ccacttattg
     3001 caaacccaaa aacagcgctt cttggaggag ctgcccagct tggtgtattc gtcacacttt
     3061 tcggtgttgc acttatgaac cttgttcctg gtgttgacta taacatgctc gaagcttgtg
     3121 caattgctat tattggtggt gcagatggcc caacatctat ctatgtttct ggaaaacttg
     3181 ctcctcatat gatggcagtc attgcagttg cagcgtattc atatatggca cttgttccta
     3241 ttattcaacc accaattatg aaaatgatga ctacaaaaaa agaacgtgaa attaaaatga
     3301 aacaacttcg acatgtttca aatgttgaaa aaacaatttt cccgatagcg cttctcgttc
     3361 tttcaattgc ctttattcca acagcagcac cgcttattgg aatgctcgca ttcggaaact
     3421 ttgtacgcca atgtggagta gtagaccgac tttctaaaac aatggaaaac gaattattga
     3481 acattgtgtc aattcttctt tctctcggtg ttggttcaca aatgactcct gataaaatct
     3541 taaacccaaa tgcagttgga attatcgttc ttgggctcgt agcttttatg tttgcaacct
     3601 tcggtggtct tttaatggca aaaatcatga accttttctt aaaagaaaaa atcaatccac
     3661 tcattggttc agctggtgtt tccgcagtac ctatggcagc ccgagtttca aataaggttg
     3721 gacttgaata cgatccttca aacttccttt taatgcatgc aatgggacca aacgttgcag
     3781 gcgttattgg aaccgcaatt gccgctggtg tattcattgc aacctatggc ggttaatatt
     3841 ttttgttttt tataaactga aataataaaa cggatatact ttttttggtg tatccgtttt
     3901 ttttagttta gaattgactt ttaagaatct aagagtatac ttattatatg atacaaaaaa
     3961 aactgcgatt tcttacttta attttcgcct ctttaatctt tttatcttgc acttccatac
     4021 aaaaaacagc tatgaattct ctcgcatcga atctttctgg tgctgataaa aaaggaaagg
     4081 cgctgaataa aaaaaacaac gagaacctca tgcttccctt gacaggagaa actgacagca
     4141 ttctaatggc agattttttc cctacagcac ttaagctcta tgaaatctta catgcacaaa
     4201 atcctgaaca tcaaggcttg agtcttatga cagcctcctt atctgtcatg tatgcaaatg
     4261 cctttattca gcataaggct gaaatgcttc cacaagaagc ctatctcttg caaaatgcag
     4321 agtttcaaag ggcaaaaatg cattatttga gagctagaaa tcttgtcttt tctgtttttg
     4381 aacaacgcta tcccggcttt tcaaaagcgc tactcggttt tgatatagac gcaattgaaa
     4441 actatgtcgc tcgtttaaag caaagtgatg tagaggcagc ctactggtta ggtgcggctt
     4501 ggcttggtgc ctggtccctc gaccctctcg acacaagtct tttaccaact gtgcactcgg
     4561 cagtgcgcct tttggaaaaa gcagcccttt tagagccaga ttataatgag ggtgctattt
     4621 ggactatatt gctttctttt tatgcgagtg caccggcaga ctttggtggc gatacagaaa
     4681 gggcctacta tgcctatgaa aaagctttgg aagcttcatc tggaaaatcg ccgggagtgt
     4741 atatagccta tgcagaaagc atctgtgttg cagcgcaaga tgctgaaggt tttatcgatg
     4801 cacttcaaaa agccttggct atcgatgcaa atgaaaatcc tgcaacacga ctacaaacaa
     4861 taattgctca agaaaaagca aattatctta tggatacaat cgattattat tttttatttt
     4921 gggattagga gagttttata atgaaaaaat ctacttttgt ttttattctt ttttgcctgc
     4981 ttagcctctc atgctttgct caaaaaaaaa tcaccttaaa aattgcttct gttgcccccg
     5041 cacgatccgc atgggaaaca gaacaaaagg ctttggccgc tgaatggagc cgtattacaa
     5101 acgggcaggt tgttctgcag ttttacaata caatagcgct cggtggcgaa gacggagttg
     5161 tacaaaagat gcgcccgcta cgtcctggac agcccgcacc acttgacgga gctattttta
     5221 caaacattgg tgcctatgaa ttagctccag agtcaaatat cttaacttta gcaatgcctt
     5281 ttttatttaa gaaccaagag gaagttgatc ttgttttaga agccttatcg tcagaaatta
     5341 gcgatgcaat gaaggttcaa ggttttgagc ttttaggctg gttcaatgtt gggtgggcaa
     5401 atttttatac aaaaaaagaa atacgaactc ctgaagaact aaaaaaaatt aaattaagcg
     5461 ttggaggtgt aacaagttct gagttaggta aagcctttca aagagcaggc tatatgacag
     5521 aagatgttcc aaacgataaa atattgcaaa gtataaaaag tgctaatggt gtacaaggtt
     5581 tatacacgat acccatgtat gcctacgctt cgcaatatca caaatcctta aactatgttc
     5641 taaataccgc catttgtccc gttttagcaa attttgtggt atcagaagcg agctggaata
     5701 aaattccaaa tcaatacaaa gatgatttac tggcagctgt taaacagtca gaagagaagt
     5761 tcattggttt tcagcaaaaa acagataaag aatatctttc tcttatggaa aaagaaggct
     5821 tacatatagt taatttgaca gaaaaagaaa ttgctgtttt tcaagaaaca ttaaaaatgg
     5881 atgcactctt aatgtcggag tcaaaaacag aaacaattat taattatgaa atgtatcaaa
     5941 aaatttcagc tatcttaaaa aagcaccggg aaaaataaaa tgaataaaaa aaaaattgga
     6001 ctgagattat ttgtacgaaa ttttgaaaat tatttttctc tttttatagt tgctttttta
     6061 attatcctgc cccttttgtt taaaataatg aatcgttttc atttccaaat actcaatagt
     6121 gaaaacgcca tcgtaaatct tgtttttgcc ttcgcctgtt tttcgggtat aattacctgg
     6181 cgagaaggaa atcatattag cctcgcgagt ttaacggatg gtctgccaaa acgttttaag
     6241 ccttttatac tttcatttag aagtgctact acagtaacta ttttaacagc tcttttttta
     6301 gcttcctttt cagcttcttt taccgccttt ctgcccagtc aaaaagtttg gggtattcct
     6361 taccttgttt tttttgcctt gctaccactt gcatatttta ctatgcttct acgaaacctc
     6421 ttccaaaaag aatatcgctt acaaaatctt ttaggctttt tcatcggtgt tttaatctct
     6481 ctcggtccct tgtcaggagt tttgtactcc ctttttaaga cggaatctct tggcttttta
     6541 tatagattaa atgattactg gcttacattt tcagcccatg cgtttatacc tcttactctt
     6601 attcttgttg tctttgcctt tataggggtg cctctttttg tcatccttgg tggcatcgcc
     6661 tatcttgcct tttcccaagg cggtggttat gtagaagtgc tttcctttga ggcatatgca
     6721 gtgcttacag ataaggcgat tgcagctata ccattattta ccatagcggg ctttatttta
     6781 tccaagggaa gcacaggaaa gcgtcttgta agcgtattta agtccctctt cggctggttt
     6841 aaggggggaa ttgtaatagc agccgtcctc gttgttacat tttttactat ttttaccggt
     6901 gcgagcggtg ttaccatact tgctctagga ccattgttag taatcgccct cggaggctct
     6961 ggttacaaaa aagaagactc tgaggcattg atcaccgcct caggcgccat cgggcttttg
     7021 tttcctccaa gtattgccat tattatgttt ggctctgtaa attatttttc tgtcgatgtc
     7081 tacgatattt tcaaaggtgc atttttacct ggtttaattc tcgctatttc aatgattctt
     7141 ttagggctga gtcgtgatgt gcaaaaagaa cgggacgctt tcaacggaag agaaatacta
     7201 tctgctttaa aatcaagcgt atttgaaatc ttgcttccct tctttgttat ctttggattc
     7261 ttaggtgggt tttttagtat tattgaagcc gcatccttta ccttgttgta tgcctttgtc
     7321 ttagaaacct tcgtgcgaaa aaccttttct ttacgagaat ctttagtcgt tataactgaa
     7381 gctattccca taactggtgg catccttttt attttggcaa gcgcacgtgg tctttcctat
     7441 tttatgatag atgcaaatat tccagagctt ataaccagtt ttataacaag tcatattcat
     7501 tcaaaaattc tctttttact cttggtaaat gtttttttac ttctcgctgg ttgtctcatg
     7561 gacatatatt ctgccatatt tattgtatct cccttgctta tgcccatctg tgagtctttt
     7621 ggcattcatc ccgtgcatac cgctgttatt tttttgacaa accttcaact cggtttttta
     7681 acaccgccga ttggtatgga cctctttgtt gcaagctatg cctttgacag ccctgtaatg
     7741 aaaattgtca aaaatgtctt accctttatt cttgtacaag cggttgtttt attgcttatt
     7801 acatatatac cctggtttac aactgttttt ttataatgcc cttaaaataa tgagatatct
     7861 acgattttta annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngagcttttt
     7981 tgccccggcg gttttatttt cttttcatat aatattggaa aaacagccga aacacttatg
     8041 ggttttaccg attctattct tgattattat aaaagagata ttcctccttt tttgtccctt
     8101 gaccttgaag gaggggaggt gaatcgcctg cgaaatatta tttcaccgct tccttcagct
     8161 gaagaaataa gtcagctttt taccgcagag gaggcagctt tattttacaa agagattgca
     8221 aaacaaagtc atttgctcgg aattagcctc aatcttgcac ccattgttga acctctttta
     8281 gaggaaaatg cagctttttt aaagacacgt tcttttggat ccatcgaaaa aacaaagttt
     8341 ttttcactcg cctttattga atcttttaac agtgaggggg tgctttgtac tttaaaacat
     8401 tttcctggta acacagatgt agacccgcat ctaaacctgc ctgttttagc gctaaacaaa
     8461 gaggactttg aaaaaaacat gcttgcacct tttcgctttg tgctggaaaa tcaaaaaaac
     8521 aataatgcgg tacttttatc tcatgcactt attcctttta taagcgactt gcctgcaagt
     8581 ctttcttcag aagtcgtcga ccttcttgta aaagacctcg attattctgg actcattatt
     8641 acagatgata tttttatggc agccctacaa agtttttttt cagaagaaaa agatgctatt
     8701 cttttggctt tagatgctgg tgcacatatc ttattgagct caaaaaaagt cttcacgcag
     8761 tttctccctt ttttggaaga aatttataca actgaaacgg tatataaaga aaagatagat
     8821 atcgctttgc agagaattat acgggcaaaa attgaagcag gtttacttgg attttctgaa
     8881 cgagaatctt ggtcgtcgct ttgtttgctt aagggaaata catctacgag catagataaa
     8941 atcgatcaaa tcgatcgaaa agaaagagaa agactttttt atgaagctaa aaaaaatgct
     9001 tccgaaatac tttcagaggc atatcaaaat aatttcttct aaaagggtaa gatggcaaaa
     9061 aaaaacaaag caaaaataga ttttgatgcg tattatacag aaatatttgg cgagcgatgg
     9121 gctgttttaa aaaaaaactt atctgaaaat ccctatagtg taaaaatcca gtttcatgaa
     9181 agtttgagcc catattatat ggatgcagga agtctctttg cagcatctgc actccctcta
     9241 gacaaagcca aaacagttct cgatctttgt gctgcacctg ggggtaaaag tctcgtttta
     9301 gcagcctctt tagaaaatac gagcagcctt atagccaatg aattatctat gcaaagaaga
     9361 gagcgccttc tttctgttct acaacaaagt ctgccagaaa atattctcga aaaagtacgc
     9421 gttactcctt acgatggtag gcttatgcat aaacgaaaca aagaacaatt tgatgcaatt
     9481 ctatgcgacc ttccttgttc ctctgaacga catgttcttg catcgccaaa acattttcaa
     9541 gaatggtcga cgggaaggat aaaaaatctt tcatacacac agggcgcact tctttcttca
     9601 gcttttttaa tgttaaaacc tgaaggtttt ctcttgtatg cgacatgtgc tttgacaaag
     9661 atggaaaatg atgatgttat agaaaaactt cttaaaaaaa atgcgaatgc cgctgtttta
     9721 gatatagaga ttgaagaact aaagggaaaa attcaaaaaa actataaact ttctttagag
     9781 atagagccag agaaaacaga atacggctat catattttgc cagacagttc aaacggtgct
     9841 ggtcccttgt atgtatcttt aattcaaaaa gtactttaaa actagattta aatgtaaaat
     9901 tgatttatac tagagtacga ggttagtttt gaaacgttct actaaagaag aaaaattaca
     9961 aagcattata cagtctgaaa aatttctcaa tcaaacacag gacttagatg ttcttttaga
    10021 aacgctttta actgaggcgc gtactatcgt aaatgcagat gctggttcta tttatgttgt
    10081 ggaagaggat aggctgcgta tcaagtacgc tcaaaacaat acacagttaa aaaagctttc
    10141 tgcgggcgaa aaattgccct ttgtttcctt ttcatttcca atgaatgaat actcaatagc
    10201 tggttatgtt gcctcaaaaa aagaaatgct aaacatagcc gatgtgtaca atatagcagc
    10261 tgattctacc tttaaattca atacacagcc cgataaagat accggttata gaacaaaatc
    10321 tatgttaacc attccccttg ttgctgcgtc aggtaagaca ttgggtgttt tacagattat
    10381 aaatgctcaa gaccaaaatg caaacacgat agcctttgat gaaaatgccg aattatatct
    10441 aaatcatttt gcagcaaatg caactattgc actagaaaga gcttcattaa ctcgtgcaat
    10501 gatccttcga acaatcaaaa tggcggagtt tcgggaccca aaagaaacag gtgcccacgt
    10561 taaccgagtt gcaagttttg ccgttgagct ctatgatcgc tgggcctttg aaaaagaagt
    10621 cccttttgaa atacaacata aatatcggga tacgctcaga attgcagcta tgttgcatga
    10681 tgtaggcaag gtggggattt cagattcgat tctaaaaaag ccagcacgat ttacaccaga
    10741 agaacatgat attataaaag ggcatgcagt tataggagcc gctcttttta atgacataga
    10801 cactgatata gaaaatatgg cacgagatgt tgcacttcgt catcatgaga gatgggatgg
    10861 gacaggctat cccggaaaaa ttccacctga agttctagag aatcccttta caccctatca
    10921 aatcaggcag ggtttaagcg gagaagagat accactttct gcacgaattg tagcccttgc
    10981 ggatgttttt gacgctctct cttcaaagcg tgtctataaa gattcgtgga aagaagaaga
    11041 tgtatacgaa gaaatccgta aatcttcagg tacgcatttt gatccagatt tggtaactat
    11101 gttttttgct atactacctc gaattaaaga gattcaagca atgtggcctg attaaaaatc
    11161 tgtcttgatt tctttcaatt ttttttgaat aagacggtcc aaggcatttg caaaacggtt
    11221 catttctttt tttcccaatt gtgtatgact ctctgctttt agcccttgca taaaaagatc
    11281 taaattcaaa tttcgtaaag caagcttttc tcctatgttt tcctcggtaa aaatatcacc
    11341 ccggtcgtta aggcatgtca aacctttcga tacgagcctt gctgcgagag gaatatcgcg
    11401 tgttataacg aggtcttcgg gagatgcatt ttcgacaata taatcatctg cagaaccttc
    11461 atcagtagaa gtaattatca ttttgaagta ctttgcttcc tcatgaggta tcattcggtt
    11521 agcaacataa tagagtggaa tttctaagcg attagaaaag cgaatgagaa gttttcttat
    11581 ttcaacgggg caagagtctg catcgaccca caagctaaac attattttat agcagtatct
    11641 attttttgaa ttaaggaaag cgttattctt tccagttcat ttaattcgtc agaggggagt
    11701 ttttgtttgc cagaaatttt ttctttgtat aactcatcgc agagcataat gccagcgaga
    11761 attgcggttt taagagcatc agctcctgcg acattttcca cctgttttac catttgacta
    11821 tagtaggcaa gcagcgtttc taagtaatca ttgctttcat ctgcttgaat tgcaaaggag
    11881 gtacctaaga tatttatttg cagtgtaccc atacttacct ttaaaaaata tcaaattcgg
    11941 tggtttcgtt ttttgaagaa ggaatttcta tttcttcttc aatttctaaa ctaaagtcat
    12001 cgtcgatttc tgtgctaggc tcatttatag cttcctcgtg ttttgtttca gtttgtgaac
    12061 cgtgtaattc ttgttcagct ggagcttgca cttcatcgat aacttgattt gctgtagata
    12121 tctctacttc ttgtgatttt acagtatgcg agatgctttc ttttataaca gcagattcta
    12181 tcgtgttaag tttgtcaagg gcatttagaa tgcccttttc aatttgttct tggtcagctt
    12241 caaaggtaga aattcgatag gctaagtttt ctttttcttt ttttaaagca ttatttttat
    12301 cgagaagcat tttgttttct tcttctagac tttgtagcct ttttaaaact tcttcaattt
    12361 tttcttctag taagtgaatc tgaccaagag taatcataga ctatgctcca atagcttttt
    12421 ttgctgtatt gatcactgct tcgaatgcaa cagggtcttc gatagccata tttgaaagtg
    12481 ccttgcggtt aataataaca ccagcctttg taagcccgtt cataaaacga gaatatgaaa
    12541 gtccttgatc acgtactgcc gcattgagac gagaaatcca taggcttcgc atatcacctt
    12601 ttttatcttt tcgtccgaca tatgagtcag tgagtgcttg cattaccgca tcttttgctg
    12661 ctttaaagtt tgtacctcgt cttcctcgga aaccctttgc aagtcctaat attttttttc
    12721 ttttggtgtt atgttttgtt ccacctttaa ctctagacat aatatctcct aaaaaaaggc
    12781 aaataaacct ttttaatatg tattgttata ttttttacct tgtgttatga ggatttttat
    12841 ccatagggta aaagttgttt tctcaccttt gcagaatctg ctggacttag aataccggcg
    12901 ctacgtagtt gtcgctttcg ctttggagcc tttttagtca aaatatgtcg aaggttcatt
    12961 tttttatatt ttaccttccc ggtacctgtt aaagaaagac gctttgcagc ggcttttttt
    13021 gttttcatct ttggcatttt tatttccttt ttattttttt ggcttcggtg caagtgtcat
    13081 agacataaat cggccctcca tagcggcagg tttttctaaa acgtattctt catcgagcct
    13141 ttttaacact tcatcaagaa caactaaacc aagttctgtg tgagcaagtt ctcgtcctct
    13201 aaaccgaacg gtaactttca ccttattccc ttctgctaga aattctttta cgtgttttga
    13261 tttaaaatct aaatctcctg aaccaatttt tggttgcatt cgaatttctt ttaacttaag
    13321 taatttttgt tttttcttgg aatcacggag ctttttttcc atttcaaaac gatacttacc
    13381 atagtcaagt attttacata caggtggatt tgctgttgga gcaatttcaa caaggtcgag
    13441 ctccttttcc ctagcgagtt tgagagcttc taaggtaggg acaatacctt tttgttcacc
    13501 ctcatcatca atgagtctta cttctcgtac acggatttgt tcattaaccc gtaaaccctt
    13561 attatctgcc aacgatcctc ctactcctat aaaaaaaatc ttcgaaaact gaagttttct
    13621 cgatgttttt ttatggtcta tttaaattca tatttaatca taccaaaaac ctctatagtt
    13681 gtctagtata aacgcttttt ttttgtcttt catcttgagt ttcatttcat ttcatgctat
    13741 ttttcaaaca tgtggttatt tagttttttt ttaataccta tcttatttag ctttgatttt
    13801 tttcaaccag aaaaagaaaa tcaaaagtcg tacttgagat attttattgg tttactttta
    13861 gcgataggct acagtgttct tatcatgctt atatattcgt ctcgtgaata tactcctgca
    13921 aaactttggt atctttctta tcgaaattta cttctttttg tattgcctct cgttatagtt
    13981 tttattctat actttcttgt ttctgtgaaa tctttttttg gaaaagtgct gtctcttgta
    14041 gatctctttg caggttttta tacaattttt atccctttct atgttttaag ttattttgcg
    14101 acacaaacat ttttttcttt gtttgttttt cccattcttt ttacggcaag tctttttctt
    14161 cttagctatt ctttatctgc ctttattaaa ttgataaata cacgaggaag tcttctttct
    14221 tttatactca ctgctttttt tatactcata gccttggtac ttccgagcct tttacttgct
    14281 ttatggacac ttggtatttc tacgatttta ttgtctatct ttgtgctgct ttatttaacg
    14341 agtgctggtg tattgatttt tttatacaaa aaaatttggt aaaaaataga gcaaaaaaaa
    14401 gctagaaagg taatcctttt ctagcttttt ttatttaggt ttaaacgttt agttagcgat
    14461 agctcccgca gaacctaaga cttctttgtt tttgtgcata tacaattttt ttaactcatc
    14521 gcgagcagga ccaagatatt ttcgtgggtc aaattcttct ggatgttctg caaagacttt
    14581 tcgtactgct gcggtcatag cgagacgacc atctgagtca atattgattt tgcaaacagc
    14641 agattttgca gcttttcgaa gttgctcttc aggaataccg acagaatctg aaagttttcc
    14701 accaaatttt tcgatagttt taacatattc cactggaact gaagatgaac catgtaaaac
    14761 gattggaaag ccaggaattc ttttttcaat ttcttcaaga atatcgaagg cgagtggagg
    14821 tggaataagt acgccgtcag catttactgt acattgttcg ggtttgaatt tttgtctacc
    14881 gtggcttgtg ccaatagaaa ttgctaaaga atcacaagct gttcttttta caaagtcttc
    14941 tacttcttct ggttttgtat aatggcttac ttcatgtgaa acatcgtctt caactccagc
    15001 caaaacacca agttcacctt ctactgttac tccaaattgg tgggcgtatt caacaacttt
    15061 ttttgttaag gcaacatttt cttcgtaggg gagatgtgaa ccgtcaatca ttacagaaga
    15121 aaaacctgta tcaatacaag atttgcaaag ctcaaatgta tcgccatgat cgaggtgtag
    15181 tgcaatttct gggtttttac agccaagctc ttttgcatat tcaacagcac cctgtgccat
    15241 gtagcgtaaa agcgtttggt ttgcatattt tctggcgcct gaagaaactt gtaaaataac
    15301 aggagatttt gtttctacac aagcttgaat aatagcttgc atttgttcca tattgttgaa
    15361 attgtaggca ggaatagcat aaccgccctt catagccttt gcaaacattt cttttgtgtt
    15421 tacaaggcct aaatctttat aactaaccat aataaagact cctttagata tttatatatc
    15481 gattttaagc ttttatagga aaataatcaa tagaaaatgc aaaataataa taaaagagtt
    15541 tgacaaagcg ctcgtgaaca aatataattt aaccgatacg aagtgtaatc tacgttttga
    15601 tttttataag gagttatgaa tgaaacaggg cgttatggtc ttattatgtc tcttctgtgt
    15661 gtttttgttc tgtgtgcctg tttttgcaca gcccagtaca aagagtgttg agacggttct
    15721 aattgatgat tttgattctg ccgaagaaaa agattatcaa tgggatgttc aagcaagcag
    15781 attcgttgca gacggctttc ccaaaaaagg atattttaaa gggattccca attctttgag
    15841 gggattttat ggtgatggtg gtactgaagc tttggtttta ggtgtgcaaa ctagttttaa
    15901 tcgcaagggc gataactggt ttgaaatatt tccaacaata accgatgaaa acggcgaaat
    15961 tgccaattat gaaattcctt tcaagggtgt tgtatcgcaa attgactttt gggtttgggg
    16021 ttcaaactac aaatatattt tggaagtcct tgttcgtgat gctgatggcc gtgttcatac
    16081 actgcctgct ggagtcttac tttttaatgg ttggaaaaac tttgtagtga atatcccagg
    16141 acatattcga caagaatctc gtttaagaag tggtccaaag aatatgactt ttgtaggatt
    16201 taaagtacgc accgatccaa atgagtatgt tgacaacttt aacatttact ttgacaatct
    16261 aaaatataca acacatacgc ttagtacgat ttacgacgga tttgaccttc gtgatattga
    16321 ctttgattct gctaatgagg agggaaaata atgaaaaaga tcgttttatt agtctgctgt
    16381 cttctttctt taggtttagt ggtggcacag caaaatggga atacaagttt gtcagatcca
    16441 gatccaacga tgattggtgc tgattctgca aaacaatctt tacgagaagt atctgttgac
    16501 ctttttgaaa gagaaggcgc ttggaatgca aaaatttcac cagactatgg tgttatttct
    16561 gctcgtcttt ttgaaggttc tcctgcggga aaagaagttt tagatgatgt agaaaatcaa
    16621 caaatatccg atgatcgcgt tttaggtgta aaggttgaat tttttagaag gggtataaac
    16681 tctttcttta tcactgctgc ccgacctatc gctatagaag gtacaacaaa aactatttcg
    16741 gtttgggttg ttggacgaaa tcaatctcat aatttaactg ttttagttca agattatttt
    16801 ggaaataact ttgagcttta catgggacag ttaaattttt ctggttggaa aaagctcact
    16861 gttgctgttc caccttcacc agatggtgtt catggcattg tacaaacaag tgcctatcat
    16921 ggagatagac ctggtttacg tatcgttggt tttagagttg attgtaatcc agaactcgct
    16981 cgtggctcat attacttata tctagatgat ttacgcgcag taacagatct ttatgctatg
    17041 gaaaatcgcg acgaagatga catggatgat aactggtaag attctttaag tcactatttg
    17101 ttaattatag ataaaaaaag ctatcactca tttatggggg tagctttttt ttatttaaaa
    17161 actcttaaat gtattggtag tattttgtat ttcctttttt ttcaactagc ctaatgaggt
    17221 ccagtttatg caaaacccat agcactttgt gagcattttt ctttattgca gttttactca
    17281 aatcttttgc acaaaatagg gcagggagct cgggcataag ctggacaagg tcctctttgt
    17341 tttttatgtg taagtgatca tttattccaa gtaattgttt atcgagtgaa atccagtctt
    17401 ttagatagcg gcgggtattt gttttatttt gtgttttttt tgcttgtttt tctcgaattt
    17461 ctacttgttc aacaaagagg acttctagga cccagttttc cacagcaaag ctctgataaa
    17521 atccaaggag ttcagaaaat aagctgtaga tatctgcctt tttgggactt tttctttctg
    17581 ataggagaca agcagatgaa tcgattgttt ttattattga ttttgttatg agaggatgga
    17641 caatgcgaaa agaatatcct tttttactga gttttagtat ttttttattc aatgcagaca
    17701 aatgtcgtgt ttgaatttct ataatttcat tgttgcttgt aagtaggtcg caaataaaac
    17761 cctcgatttt cacctcttct tctgccttat attttttttt gtaaattttc tttagcgatt
    17821 tatgtagtga gctttcgtta taggtttgaa tcatttctat actatacatt tgtttagcat
    17881 aaaagtaaag aatagctcta aagggctata aattttggtt ttcattgttg actttagcat
    17941 aatctgctat aaaatgtatt tatgtgggaa ttagtggtaa aaagtggtga atagtggaat
    18001 gaatcttata actggtgaat atcgtaatac tttagacgaa aaagggcgaa tcctttttcc
    18061 ttctcgatta cgctcagagc ttaaggaaac acatctcatt attacacagg cgctcgatgg
    18121 ttgcttgtgg ctttttactc ccgacgaatg gagaggattt tcatcaaaac tgatggaatc
    18181 cgcctctcca tttaacagca attcacgttc tgttttacga cgcttagttg cgccagcaca
    18241 agagattgaa ttcgacaagt ctggtcgact ttctatacca caaagtttac gtgaatatgc
    18301 aggtcttgaa aaagactgtg ttattttggg tatcaataaa tttattgagt tgtgggatgc
    18361 ggaaaaatac aaggcatatt tagataaaac gcagggttca tttgatgagg caactgaaga
    18421 attgagccaa atcttttttt aggggatgaa ggggtggaag ctgttcatac atcggtttta
    18481 ttgcaagaag ttctcgacta tttagatcca cggcatggag aaaatccaga aagtcctttt
    18541 ttgattgact ctactttggg tgaaggggga cattcttttg cttttttaaa ggcttttcca
    18601 caactacagg ttttgggtct tgatgctgat gcagaaattc aaaaaagagc caaagagcgc
    18661 ttaaaagagt ttggcccaag gatgcggttt tttaggggat ggttcaatga tttttaccgc
    18721 aattatccag aagaacttgc gaaacctgac cggattcttt ttgatctagg catttctatc
    18781 tttcattatg agctttcaga gatgggcttt agttttaata aagacgaacg gctcgatatg
    18841 cgtctcaatc atgatgaaaa aatatctgcc tatgagatag taaatgaaag tttagaagaa
    18901 gaacttgctt ctattctttt tttcaatgcg gatgaacgtt atagcagaag aatagctcgg
    18961 gcaattgtta cagagcgtga aaaagctccc gttgagtcaa ctattgcctt acgagacatt
    19021 gtgtataggg ctgtgccggt tgcatatcgt catggtttta cgcatcctgc tacaaaaact
    19081 tttcaagcta ttcgtgttgc ggtaaatcac gagtttgatc gcataaaaca agcgcttgaa
    19141 gatgctttta tgcagcttaa accaggtggg cgtatggcgg ttattagttt tcattcaatt
    19201 gaagacagga ttgtcaaaca tacttttaga gatttgggga aaagctgtgt ctgtccccct
    19261 gatatgccga tatgtaaatg tggaggaagt cctcgtgcaa aaatcctcac gcgaaagcct
    19321 attgagccga cgagtgaaga gatagaaaaa aatcctcctt cgcgatcagc aaaattgcgc
    19381 gttatagaaa aaatataaga ctaggagtga taggggtaaa ataatggttt taaaaaaaga
    19441 aaaaaaagtg tctcgagtaa gtatttattt tatagccttg cttattcctt cttttttatg
    19501 tctcgatagt atgcagtccc ttaaatatgt ttcaattcaa agggaaattc gggagcttga
    19561 aaaaacagag gcaaaactca tcgaaaaaaa taaaactttg attgctgata ttagtaactt
    19621 gagttcaaca agtcgtattg aagcaattgc agtgaatgag cttgcaatga gaaaggcaac
    19681 aagtagtgaa attatgcgtg tcgaggttgg gcgatgagaa aaatgaacga aaatacattg
    19741 actaatcttt taagcctcag tgaagtcctt cttgcaaccg agggcaagct tttaaaccct
    19801 acatatagtg aagaagactt tgtttttact tcaattacaa ttgatagcag gaattgtgtt
    19861 gaggggagct tttttgtccc acttgttgga aatctagacg ggcatgattt tattctttca
    19921 gcagccgaaa atggcgcaaa gactgttttt gttagtgatt tttatgcaaa gtttcatgaa
    19981 aaagaaatag catcttttat agattctggt ctttgcctta tttctgtaag ccacacaatg
    20041 tatgccttgc aaaaaatggc agcgtattat gtggaaaaat ttccaaatct caaaaaagtt
    20101 gctataacgg gctcaagtgg taaaacaacg accaaggaaa tccttgtttc tcttttacaa
    20161 aaaaaataca agactgtgta taacgaagga aacttaaatt cagaaaccgg cttacctctt
    20221 tctgtttttg ctattcgtga tgagcatgaa tgggctgtct ttgaaatggg catgaatcgc
    20281 cgaggtgaaa tacgagaact tgcacaggtt cttaaaccaa atattgctat aattacaaat
    20341 attggttctg cccatatcgg taatctcggc acaaaggacc tcatagctga ggaaaaaaaa
    20401 tctgtattta gtttttttga aacaagttca attgcaatca ttcccgtttt tgatgaatac
    20461 agcgaatatt tacaaacaaa tacaaaggga caaattgttt tatatggcaa ttatgaagct
    20521 ttacatattc gggctgtaaa agatcgtggt ttagaaggta gcgttttttt aatggaagac
    20581 caagaagtat cctttccctt atatggttct tttaattttc taaatgctct cgctgctctt
    20641 cattgtgcaa aaattgccgg cttgggtata aatgagatta agcgtggttt agaagagctt
    20701 cgcccatcct ttggtcgtgc agaaatattt aacggagaaa ttgccattgt acaagattgt
    20761 tataacgcaa accctgaatc gatgaacgct tccatcgaat attttgaaac gctgcattgg
    20821 gaagggcgaa aaatcttgat tcttgccgac atgcttgagc ttggctttga ctctgaaaca
    20881 caacatcgtt tagtggggaa aaaactaaaa agcctttcct ttgatatgat ttttttattg
    20941 ggtagtcaaa tggactgggc ctacgaagaa ttgatgaagg ggcagagtac aggcgaaaat
    21001 actgatgaaa aaaacgagaa aaaaatatac tacaatgcct ttatctcgga tgctgatttt
    21061 ggaactgcgg taaacgatat tttggtacat gtcaacaagg gcgatatact tcttctcaag
    21121 ggttctcgcg gtatgaaact agagcgtatt agtgaagcgc ttgttaaagc ttttaacatg
    21181 caaggttgta ctatatgcta gaaatgaacg ttgtgccaga aaaagctgta gaaaaaaaca
    21241 cgagcgatat gtactttatt gcaacgatgc ttttgttgtt ggggcttggt ttagttactt
    21301 tatttacctg ctcagcctcc tatgcaaacc gtatctttga cgattcattt tattttataa
    21361 aaagacaact gatttcttgt tttattggct ttatagtctt gtttatttta tcatacatcg
    21421 atatgcaatt tgtacgaaaa atgcttcctc ttatcgttat agggaccttt tttctttgtc
    21481 ttttaaccta tctcccaggc attggcgttg agcgaaacgg agcacggcgt tggattcgtc
    21541 tgccctttat ttcgaacttt caaccctcgg agttagtaaa aatagcaatt gttttgtatt
    21601 tggcaaatct ttttgataaa aaatatgaaa taattaacga tgtaaaaaaa acaataatcc
    21661 ctgcagtctc tggaattatg ctctttgttt tcgttatctt tttgcaagat gacttttcaa
    21721 cagctttttt tattttgctt gtaagcgtga gtatgttttt cctcgcggga ttgagtttgt
    21781 attggttttt ttctttattc ttggtttcaa tccctgtttt gatacttttt atttttacag
    21841 aaagctatcg agtaaatcgc attctcgctt ttttaaatcc cttgcaagac ttacacggta
    21901 tcaattacca aataaatgca tctataaagg ctatttcgaa tggcggcttt tggggggaag
    21961 gttttggcat aggtttgcaa aaatcaagcg caatacccga agtgcaagct gactttattt
    22021 ttgcaggctg ggttgaaggc atgggtttgt ttggcgttct tttatacctg cttttgcttg
    22081 gcttttttgc atggcgaatc tacctcatcg cttttcgcgc agaagataga ttttcaagct
    22141 atgtcgcctt cggcttggga acgagccttg tcctgcagtc tctcttgaat atcgcggtgg
    22201 ttgttggcgc tgttcccgca acaggcatcc cccttccttt tttttcgtct gggggttcat
    22261 ctttgttaat tagtatggcc atgtgtggtc ttataataaa tatttcaaaa aataataaaa
    22321 aagaggcaaa gtatgagtga tggattaatt tttgacaact ctgaatcatt ttgtagcatt
    22381 gaaaaaaaag aaaaaaatca aaaaacaaag cttcgtatac ttaaaatact tttttttgtg
    22441 ctattgagtt ttgttttaat tgaacttatt ttgtatagcc tcgtaattcc ggttttttca
    22501 aatatccaat tttctgttac agggcttgaa acgctctctg aaaaagaaat agcacagtat
    22561 attcagcttt caagcaatga tacctggttt aattttaata caagccgtat ttcgcaaaaa
    22621 cttgcatctc ttagtcaggt tgaaacggta agtgttgaaa aaagatttcc agatcgtgtt
    22681 gcaattcata taaccgagcg aaaagcggtt gctttttctc ttgcgacaat acagggaaaa
    22741 acggtaccgg tacaaatcga taaaaaaggt gttgtttttg ctatcggtaa agaacttgga
    22801 aatacagcca tacctcttat aacgggcttg gccttagaca atatttctga gggagcacgt
    22861 ttacatgcaa aattgcttcc actcatagaa caaatttgtg aaattcaaat aataaattct
    22921 gattatttta atgtattatc agaaattcgg gttttaccaa aagactatgg cgcatatgag
    22981 ttagcactct atccattaaa tacacatatt cggtttttat cggatagtgt attgaatgtt
    23041 gaatcattgc agtatattat ggtatcatta gatgtattta aaaaaacaaa tacaatggtg
    23101 aaagaagttg atttaagaaa tggtgtaaca tcttttaaaa cattatagta aaagaactta
    23161 cctgggagga gtatcttgag tgacattatt gtcgggctag atattggtac aagtaatgta
    23221 cgagttgtaa tagcagaact aaatgaattg ggaaagctac aagttatagg cgtgggaaaa
    23281 agcctttcga caggcttgcg tagcgggctc gttgtaaaca tcgatgcaac tatgaaggct
    23341 atagaaaaag ctgtagaaga tgctgagatg atggcaggtt gtgaagttaa atctgttttt
    23401 atttctcttg gcggttccca gatcgaaagc caaaattcaa agggttttgt tgctgttgca
    23461 caaaaggcca aagattctcg cgaaataacc gaagatgata ttgcccgtgt aattcaagct
    23521 tctcaagcta ttgctatacc acttgataga gaaattctcc atgttatacc acaagaatat
    23581 attatagatg gaagtcaaag aacaaaagaa cctttaaata tgataggcgt tcgacttgaa
    23641 agcgaggttc atattataac cgcctcagtt tctcctatgc aaaatattat taaatgcgta
    23701 aaacgtgctg gctacgatat agatggaata atgctcaaaa ctttggctgc gactgaatcg
    23761 gttatgactg aggaagaaaa agaccttggt tcgattttaa tcgatttggg aggcggtact
    23821 accgacgtac ttgttgttct tggaggtgct cctatttgta cggcaactat tcccatcggt
    23881 ggtatttttg ttacaagcga tatttctact gttcggggag tgtctattga gacagctgaa
    23941 acgataaagt tgagtgatgg gtgctgttgg gaaggcttat taagtgatga tgagtatttt
    24001 ttgattcccg gtgttggcgg aagacctcca gagcaggtga gtcgaaaaga actctgtgaa
    24061 ataatgcaac cgcgtgttga agaaatttta aaaatggtta aagaaaaagt atatcaattg
    24121 acgaagttaa aaaaactttc tggaaatgtg gttttaaccg gggctggtgc tttgatgcct
    24181 ggcattattg agttagcaca agatgtcttt gacactgcct ctgtgagaat aggtacttcg
    24241 gctaacttag gaaatgtgac agatgcgtat agaagtgcag actttgcaac agctacggga
    24301 cttcttgttg catttaaaga taggcttgaa gtaaaagctc aagggccggg agcgagtctt
    24361 cctgttgcgg agaaaaattc tattttaaag tcaataggaa gcttttttaa agaattcttt
    24421 taatatatat ggggaaaaag gggatattat gattgaattt gaaatgcttg aacaagatta
    24481 taagctcaat ccaactaaga ttaaggtaat tggtgtcggc ggtggtggtt cgaatgcagt
    24541 aaatagaatg atagctgcag gtttaaaaaa cattgatttt attgctgcaa atactgactt
    24601 gcaagcactg agcacatctc gtgctcagac aaagattgga atcggttcga agataacagg
    24661 tgggcttggc gcaggcggta aacctgaagt tggtgaaaag gcagctgtag aagatacaga
    24721 tgaaatagcg aatcttgtaa aaggtgcgaa catggttttt gtaaccgcag gcatgggggg
    24781 ggggactgga acaggtgctg ctcctatagt tgcaaaaatt gcaaaagaaa gtggtgcttt
    24841 gacagtcgct gttgtaacta ctccatttca gtttgaaggc gcttttaaaa tgcgtttagc
    24901 acaagagggt atagaaaaat taaagccaaa tgtggacacc ctcatcgtta ttcccaacga
    24961 aaacctcaaa aaagtaaatg aaaaaaattt aaccatggca aaaatgttta tgatagcaga
    25021 tgatatctta cgccagggtg tgcaagggat ttctgacctt attacgcaac atggggagac
    25081 aaacataaat attgattttg ctgatgttga aacaacgatg aagggtaagg gagacgctat
    25141 ttttggcgtt ggtgtttctc gcggtgaaaa tagggcagtt gatgcagcaa cgcaatctat
    25201 aaacaatcct ttactgcaag attcgtatat cgatggagca gaaaatattt tactcaatat
    25261 ttcttcagat gaaagcttag cctatgatga agtagaagaa attgttaata ttatccgttc
    25321 aagtgctgat ccaaatgtcc atcttattta tggacaaacc ttcgatgcaa atatggaaga
    25381 tagcataaag gtaacggtga ttgccactgg ttttgcaaaa gatcgatcga tgaaaaaaca
    25441 acttgactcc agtatgttta gtaagcagca gactaaaaaa gacattgata aagattctta
    25501 tatctcttct gatatgtggt cggaaataca aggtgaagaa aagaaggaaa aaaatcaggc
    25561 acaatatcta tcttcgagaa atcaaaatga tttagaaaca cctgcttttt accgaaacaa
    25621 atcgggtata aacttaaaac cctaaaggat tttttttggt acatgccttt gaagatgaac
    25681 tactttccgt tgatcggcgt tctcgtttaa gtgtagaaac ctatggcttt tctgcgcaag
    25741 ccttccttga ctggtgcgat acaaaagaaa ttgccttgga gaaggtgggc ttagaaaatc
    25801 ttatagccta tggcattgaa aggaaaaaag caggaataga tggggcgact attgcaaaag
    25861 atatagccgc ccttagggca tttggtgctt ttttggtccg taaaaatatc tggaaagaaa
    25921 attatgcgct tttacttgaa aaaccaaaac tgcatcgctc tttgcctcgt gttttagaac
    25981 ttgaacaagt tgaggccatt ttagatgcaa tcgatacaac aaaacctctt ggcgtgcgag
    26041 atcgctgttt atttgaaata atatattcat ctggtttgcg gataagcgaa gcctcgtcct
    26101 tacttatgca aaatctgcaa ttaaaggagc aaatactctg ggtccaaggc aagggcgata
    26161 aggagcgtat cctacctttt ggggatgaag ctctgtattg gtttcaaaag tggattcatg
    26221 aagaacgtcc taaaatactt aagcaaaaac aaagccccta tgtgtttata aactatgcag
    26281 ggggacagct cagtcgaaag ggaatttgga aaaaatttca ggatttaaag gcaaaaacag
    26341 ggcttgaagc aaaggtacat accctgcggc attcttttgc tactcattta ttgcagggtg
    26401 gtgctgattt acgcacagtg caagaacttt taggacatca ggaccttgca acgacacaaa
    26461 tctacacaca tattgatgct tcggaattgc aaaagtatca tgaaagatat tttaaaaaaa
    26521 agagcgacaa ataagttttt ttatattaga cttattgaag ggatatgcct atacttttaa
    26581 gatactagac ttattttgtg tgatgaaggg agaaaaatga aaaaacaaaa gatttttttt
    26641 aaaatagcta ggcttatttt gctgatggct tttttatcca tcttgcttgc ttgtggacct
    26701 tcagttaaat cggtaaaacg attacaattt atggaagaaa aagtttccag tccaaatact
    26761 attgaagaat tggaagcagc tgttgcaaaa tttgaaaagc gggtcctcga tataagcgag
    26821 gcaaatagtc aaatcggtat ttggtataag atgattgcga ctcgctatct cgataaacaa
    26881 atgtacaatg aggcattgaa agcgcttgaa aaagctgttt tgtattatcc tacaaatcaa
    26941 aacctgtatt attttatagg ggtctctgct ggttttatgg caaaggcaag cttagacttt
    27001 gaagcaaatg gtagcagagt caaagcagag tattatctaa aactttctga gtcagcgtat
    27061 aaacgtgcga ttgagttgga gcctacctat gtgcgttctt tgtatggctt atcggtctta
    27121 tatttatttg atttaaatga agcagaaaaa gccataccgc tcttacaaaa agtactaagc
    27181 atcgaaacaa aacactatga tgcaatgttc cttttagctc gagcttatta tatgagctat
    27241 gagtttgaaa aggcgcttga tgtatatgat aaaataattt ctctttctgc tagcgagcaa
    27301 aggacacgcc aggctgagga aaataaaaaa atcgtgctcg atgccttgta tggagattaa
    27361 aaacgatgag agcccttgct ttattgcttt ctcattacga ttttttttct ctttctgaaa
    27421 aatatcttat cttagagcag gcgaaagata tacaaacctt tctaaagatg cgaccgattg
    27481 aaattgcatc gctcatagga agagatttta aaatcgataa gcttaacttt cagtcaatag
    27541 aaaaacgcct agaaaaaaca attgctctta tgagggctta ttctattggg cttgtttttt
    27601 atgacgaaga agcctatccg cccttattaa aagaaattca cgacccgccc tttgccttat
    27661 tttatcgcgg ggaagcaagc cttttgcatt cgctttgtct gggcattgtg ggtactcgtc
    27721 gtcctacagg gcgaggaatg aaggcttcct ttgatatagc aaaaagcctt gccgaaaatg
    27781 cgctaacgat tgtttcggga cttgctcggg gaattgatgg ttatgcgcac aagggagcgc
    27841 tcgaggcaaa agatggaaaa actatcgctg ttctcgcttc gggcttagat agcatttatc
    27901 cgagtgatca caaacgctta gctttacaga tgcttgaaaa aggttcgctt ttgcttagcg
    27961 agcatcctcc tttgacacct gcgcttactt ggcattttcc caaaagaaat cgtatcattg
    28021 cagggctttc ggagggcgtg cttgttgtag aagcaccgcg cggttcaggc tctcttataa
    28081 ccgcttcttg tgctatcgaa gaaaatcgcg aacttttttt ccatgagctt gccctgcagt
    28141 actcttttca aaaacttgcc ctttctgcga gcgataagca aaaattacag aaaaaatggg
    28201 cggtacaaaa ctatattgat gacggagcat tgcttatttc tagcgcaaaa gatattatac
    28261 aacatttaag tgaaggaagt ataaaaaata ctagtttact tttttctcaa tacaagctag
    28321 atttaaacta attagatata ttttgaaact gaaggatttt ttatggctga aaagaaaaca
    28381 acaacaaaga aagtagcaac aaaaaaaaca acaacaaaga aaacaaagca aaagcaaacc
    28441 ttggttatcg tggaatctcc agcgaaagca aaaacaatag aaaaatatct cggacctggt
    28501 tttaaggttg aagcttctat ggggcacctc attgacttac caaagtctcg acttgctata
    28561 aatgtcgaag ataagtttca gccagaatat atcacagtga gaggccgtgc aaaaattttg
    28621 aaaaacttgc aaaaagaagc aaaaaaatct gaaactgtct tactggcaag tgataatgac
    28681 cgtgaaggtg aggcaatcgc atggcatttg aaaaattctt tgtctgaaaa aacagaagct
    28741 tctataaaac gcattgtttt taatgaaatt acaccacaag cgattaaaga ttcagtcgaa
    28801 aatccagctg aaataaatga atataaggtt aacgctcaaa aggcgcgacg tgttttagac
    28861 cgccttgttg gttataatct gtccccactt ttatggaaaa aggtaaaaaa tggactttcc
    28921 gcaggtcggg tacaatctgt tgccctgaaa cttatttgtg agcgagaaaa agaagttgaa
    28981 gcctttattc cagaagaata ctggagttta gaagcggagt tttccaaggg taaaacagtt
    29041 tttcctgcac agcttttcca atacaagggc gaaaaacctg ttcttaaatc tgggcaagat
    29101 gtcaatgatc ttattgcgga aatagaaagc gctcaatcct ttgtgagtga tataaaagaa
    29161 agcgaaaaaa ctatacgccc aaaggcacct tttacgacct ctaccttaca acaaactgcg
    29221 gcaaaccgtt tgggttttac ttctcgaaaa acaatgcaaa tagctcagca attatatgag
    29281 ggtgtaaatt ttggacaaga tcgcattggt ttaattacct atatgagaac agattctacg
    29341 agaatttctg cctcagcact tgaagaagta cgcaccttta taggagaact ctatcctcaa
    29401 gaattgcctg aagcagctat cgaatacgct gttggcacaa aggcacagga tgcccacgag
    29461 gggattcgtc ctacctatgt atcgtatacg ccagactatg taaaagaata tttgaatcgt
    29521 gatcaattgc gcttgtatac gattatctgg gaacgctttg tttcaagtca aatgacaaat
    29581 gcaaaaagtc gcacaacaac ggtggaaata agcgcaggcg atgcaaagtt tcgtgcatcg
    29641 aagtcaaaac tcgttgaaaa aggcttttac caagtgataa aactcctttc cacaaaggaa
    29701 gaacagtcgg gaaaaatacc ctctgtaaag ctcaacgaag agctcgaggt agaagccttt
    29761 catcctgctc agcatttcac gcagggacct cctcgctata ccgatgcctc cattgtcaag
    29821 gcgcttgaag aaaagggcat tgggagaccc tcaacctacg ctcccattat ctcggttttg
    29881 ctcgatcgct attatgttac aaggagcaat aaacaactcg ttccaacaca actcggtcgt
    29941 attatctcaa atattctcag cgattcattt ccaaacgtgg ttgacgaaaa ctttacacgt
    30001 gaaatggaag aaaaactcga taatgtcgaa gaagataccc aagactgggt tacgatgata
    30061 gctgacttct ttcatccatt caaagaccgc gtcgatgaag ttatgtcgaa ccttgaaagc
    30121 atgaagggga taatggacga accaaccgat aaaatctgcg aactatgtgg aaaacccatg
    30181 ttaaaaaaac ttggacgctt tggttttttt attgcttgct ctggtttccc cgaatgccga
    30241 aatacacgat cgctaccctt ggcaccctgc ccaaaacctg attgtggcgg cgaaatagtt
    30301 gcgcgaaaaa caaaggggcg aggaaaagag ttttacggct gtacaaacta tcctgaatgc
    30361 gattttatat cgcactacaa acccatcaat gcctcttgtc cagaatgtgg gtatttttta
    30421 gttgaaaaat acgacaaaaa aaatggctct tataagtctt gtatcaatcc agaatgcgat
    30481 tacttgcata gcacaagtga ggcagaagaa aaaaaaagcg aggaaattgc tcaagagctt
    30541 gcagaatctc aaagggctct gacaggagaa gctgaggagt agtggtgttt ttaggcgaag
    30601 caatcgaagc atttttgcag tatgcagatg gcataaaaaa tctctcacca catagcctca
    30661 aagcctatcg aaatgacctt tctttttttc tttcttcttt tccagaaaaa gaaaaaaaaa
    30721 tactttttga gataagttta atcgatatgc gttcctgtgt aggcctttta tctaagcaaa
    30781 aaaaaactgc tacaacaatt aaccgattta tctgtacgat acgaagcttt tttttgtatc
    30841 tttatagaat gaatatacta ccaaacaata ttgcgcttga cttaaaaacg gtgaaaattc
    30901 ccaagaaact acctcggtac atgagtgatt ctgaagtcga tgcacttcga agacagccag
    30961 aaaaaaaaga acttttgtgg acagaacgag acagggcgat ttttgaatgc ctctactcct
    31021 cgggtgctcg cgtgagcgaa ttgtcaaact tgaatcttcg agacttagcc gcaaatcttt
    31081 catcggcgat tgttaccgga aagggcagca aggatcgaaa ggtgtttttc tctcaagatg
    31141 cagtggatgc cctcaaagtc tacctcctgc aaagagcaga aataatacaa aaaaatgagg
    31201 taaagggcga agcacttttt atcaatcaaa gagggggacg tctaacagca cgtggcattt
    31261 tttatgtggt aaaccgctat tcttcggtgg aaggaacaaa caaacccgtt tctccccatg
    31321 cctttcgtca tacctttgca acaacaatgc taaaaaacgg ggcaaatatt cgcgtcgtac
    31381 aagagctttt ggggcatgaa aatatatcga ctacacaaaa atacacacat ataacaaagg
    31441 aacaattgat acaaacatac aacgcatctc atccgcatgg aggtaaaaat gagctttgag
    31501 tatgagaaaa ttcgcagtac aacgattatt gcggtgaaaa aagatggaaa aatggctatg
    31561 gctggcgacg gacaagttac tatgggcaat actgttatga aaggaaacgc aaaaaaagtg
    31621 cgtcgcatgt atgacaatac tgtgctcact ggttttgccg gggcaacagc agatgccttt
    31681 aatttattcg ataaatttga aatgaagcta aaagaatatt ctggagatat tacacgtgct
    31741 gcagtcgaac ttgcaaaaat ttggcgcaca gacaaggcac ttcgtcaact agaagcgctc
    31801 ctacttgttg caaacaagga caaaatcttg ttaatatcgg ggactgggga tgttatcgag
    31861 cctgaaaacg atgttttggc tattggttca ggtggaaatt atgcctatgc ggcagccttg
    31921 gcttatattg agtcatcaaa tttaagcgca aaagaaattg cagaaaaaag cttaaaaatt
    31981 gctggtaata tttgtattta cacaaaccaa aatcttataa tagaagagtt agaataaagg
    32041 aaagaaaatg agcgataaaa cagaacttac accaaaacaa atagtgagtg cgctggatac
    32101 ctatattata gggcaacaaa aggcaaaaaa agctgtagcc atagcattgc gaaatagaat
    32161 gcgcagatta aaacttcctg aagatattcg tgaagaagtt gcccctaaaa atattttgat
    32221 gattgggcct acaggagttg gtaaaactga aatagcacgg cgacttgcaa agcttaccgc
    32281 agcacctttt ttaaaagttg aagcgacaaa gtataccgag gtgggctatg tcggtcgcga
    32341 cgtcgagtcg atgatacgcg atttaatggc tgttggattc aatatggtaa aggccgaaat
    32401 gcaagaaggc ttaaaagaag cagcagagca gcgcgttgaa gaagctcttt tagacttact
    32461 tttgccacca agtccttcaa agaaaaaaga gcaaagcaat cctcccttcc ttctaaactc
    32521 ggcatttgga tcagaaaacg cagagacagt gcaaagctca caaaaaactg aatctgaaga
    32581 tgcatctacg agagaacgtt ttcgccagtg gctccgtgaa ggaaagctcg aagataaaga
    32641 agttgaaatt acaattacaa aacaaaaccg tatgccgaca atggaaattt ttgccggtgg
    32701 aaatatagaa gactttgaag aaacaatgca aaatattacc tctatgctca gcggtggtcg
    32761 cagcaaaaaa aagatgctca gtataaagga tgctcgcaga attcttcttg aagaagagct
    32821 cgacaaattg gtagatacag aaaaagtaag tgaagaggct cgaaaacgag ttgaggaaat
    32881 gggcattata tttatcgatg aaatagataa gattgcggta agtaattctc gtgggtccca
    32941 agatgtttcc cgtgagggag ttcagcgcga tattctaccc atcgtagagg gttcgactgt
    33001 taacacaaag cacggcattg tagatacaac ccatatttta tttatcgcag cgggagcctt
    33061 caatcttgca aaaccaagcg atttaattcc agaattgcaa ggccgctttc cgcttcgtgt
    33121 cgaacttgac tctcttactg ccgacgactt taaaaagatt ttaactgaac ccaaaaacgc
    33181 cttaacaaag caatatagcg agcttttaaa aactgaatcc gtaaccatcg attttaaggc
    33241 tgaagcaatc gatacaatga gtgcaattgc acaggaagta aatgcaaagg ttgaaaatat
    33301 tggagcgcgg cgcttacata ccatcatgga aacagtactt gaagagcttt cttttgaagc
    33361 agacgaaaga agcggcgaaa caattgaaat tacaagtcgc tatgtggaag atcgactgga
    33421 aagcattgta aaagatcaag acctctcaaa atatatttta taaagtattc cttcaaatgg
    33481 ccgatgttta aactgtaaca tttcattcaa atgggggatt aaatgaacag ttttacacgg
    33541 tcagttgatt tattacatag agcgatggat gtgagcacgc tacgatattc ggtgagtgca
    33601 aacaacctcg caaatgccgg agtgccaaac ttcaaacgaa cgacagtaaa ttttgaagca
    33661 gccttaaaag aggctatcga ctcagaaaaa aatgcaaaag acagctttgt gttaaaaaca
    33721 agcgatgatc gccatatcca atcaaaaacc tataccgatt atagaactgt tgaacctcgt
    33781 cgcgtactcg attatgccac aacaagcgat gcgaatggca acaacgttga tgccgaagct
    33841 gaagccatgg atattttgaa aacgcagttg aactatcaat tacttactca aatgcaatca
    33901 tttcaatttt cacaaatgca cattgtgctt aaataaagga taagaaatgg gactttttac
    33961 cagtatcaat atagcagcga caggaatgtc agttgaaagt ttacgaaggg atattatttc
    34021 agacaatatt gcaaatgcct ctacaacgag aacagcggac ggcggaaagt ttaaaaaatc
    34081 gacgcttatt ctagaagcaa ttcaagatga tttacagtat cgaaatccct ttgttcctgc
    34141 agataaggac tcaggagtag ggaagggtgt tcgcgcgctt cgggtagaaa aagacaatag
    34201 ccaaggtcgc cttgtctacg accccacaca tcctgatgct attcaaactg gtccaaatac
    34261 tggctatgtc gagtatccaa atgtaaatat cgtaaatgaa atggtgaacc ttatttctgc
    34321 atctcgtgcc tatgaagcga atgctacggt tatacaaggt tcaaaagaaa tgtttcaatc
    34381 cgccttgcag attggaaaat aaatatgcta tgattggttt gggaggactt catgattagt
    34441 tcattggatt tattgcgtta taatgcaagt acaacagcct caaatgctgt ttcaagtact
    34501 caattaagca aggctttaga aaaaacaaaa gatggtttgg gcataacaaa tactgaagag
    34561 ggaaagtctt ttgacaacta tcttttggat gctatttcct atgtaaacga ttcacagaat
    34621 aaaagctctg ccttatttga gcaggtaatt atcgaccctg attctgttga cgcacatgat
    34681 gtaaccatag caatggcaga agcaaatttg tccctatcct tagcacaagc ggttatagat
    34741 aggatgataa agggatggtc cgaaattacg accactcgat aaaagaaagt tgcttataaa
    34801 aagaagattt tagcttctaa atagcaaata ccccttgatt tatagcaaaa aaaagaaaaa
    34861 acgcttttaa gttttgagtt cttatgctat aattgaacaa tatattattt attcgttcac
    34921 gggagggcat gatgaacgaa tggcttaaaa agcttttttc acaaattaaa acagtttggt
    34981 cgaagggtag cacagtacaa aaagtggtaa ttactggcat aattatcttt gtcattggtg
    35041 caatcgtttt tatatctgtc ttatcctcat cgccaagcac tgtgccattg tataatattc
    35101 ccattactga cgaggattct cgcgatagga tccttatacg tctttctgaa gaaaatattc
    35161 aggcgacggt aaatgcagca ggtattattt cagttgcgga tgagaaaaca gcacgaaagg
    35221 cacggacgct tttggtgcga gaagatttaa ctcctacaaa tgtcagtcct tggaatctct
    35281 tcgataccga acgctggaca acgactgatt ttgagcgaaa tgtaaacttg cttaggtcta
    35341 ttacaaacca agtaacacag cacattgaat ctcttgaaga agtcgatagg gcaaatgttg
    35401 ttataacccg tcctgagaga actctttttg cagctgatca aaatccgaca accgcaagtg
    35461 ttattttata tcatagaccc ggaagcgata ttttaacaaa caaaaagaaa ttgcagggta
    35521 tacaaaagct cttgctattt gcagttgaag gtcttaaaga agaaaatatt actattacag
    35581 attcgaatgg taatgtctta aacgactttg ctggtatggc agaaattgat cgtgttgata
    35641 ttatagcgcg tgaacaaaaa cttatttcta ccctagaaac gcagtacaga gcaaaggtgc
    35701 tttcagccct gcaacaaatc tttactcctg atcgcgtgcg cgacttaaat atcaaaatag
    35761 atatgaatat gtcaaaacgt tttgttgacg caactgagta ttctcctatt actattcgcc
    35821 ctgataatcc cgacactccc tatgatgact ctgagctcgt agaatctatt accctttcat
    35881 ctgaaacggt tacaaagcgt tggactggta ctggctataa tcctgaaggt ccagctggtg
    35941 ctgaggggca aaccgctcct gtttattctg atatgtcaaa tctctatggc gtttctgaag
    36001 aaacaggggt aaaacgaaat gaagttataa atacaaagca aatccaagaa gagataagcc
    36061 cttcgattga tcgtatttct gtttcggtaa atatcgatgg tcgaaataga attgtctata
    36121 gtgacaaggg tgagccagag cttttacctg atcgctctat aaaaagggag tatataccgc
    36181 ttaccgcaga agagcttgca aatacgacac ggcttgttga agatgctata ggctatcaac
    36241 ggggaagagg cgactctgtt tctgtgcaaa acattcagtt tgaccgaaca gcacaatttg
    36301 ccgccgaaga tgcggcgctt ttacgcgcgc aacaaacaaa gaaaacggtc ttttatatct
    36361 tgagcggtat tgttcttgtc ctgctcgcct ttattatttt tagaacgata tctcgcgagc
    36421 ttgaaaaacg ccgacgatta aaagaagaag agcttctcag gcagcacgca aaaatgcgtc
    36481 aagaaacctt tttggaagca gaacgagctg gcatggaagt tactatgtct gttgaagagc
    36541 gtagacgtgc ggaactgcaa gaaaatgcaa ttgcacttgc aaaagaccat ccagaagacg
    36601 ttgctatgct cataagaact tggttgatgg aggaatagta tggcagcaca agcgaaatcg
    36661 aaaccaagcg cacccgctgg cgctacagcc tcaactggta gtaagaagcc aaaagattat
    36721 aaatctctta caggtagaca aaaagcagca atttttttag tttccttagg atctgagctt
    36781 tcttctgaaa tatttaagca tttgcgagaa gatgaaatag aaacgctcac ctttgaaatt
    36841 gtgcgacttg acaccgttga ctcagaaata aaacaagcaa tcttagaaga atttcaagag
    36901 cttatggccg cacagaactt tattacttct ggtggtattg actatgctcg cgaactactt
    36961 gaaaaatctc ttggaagcca aaaggcaatc gatattatca atcgccttac cagttctttg
    37021 caagtgcgcc cctttgactt tattcggcga actgaccccg ctcacttatt aaactttatt
    37081 cagcaagaac acccgcaaac gattgcgctt attttagcct atcttgagcc aaataaggct
    37141 tccgttattt tacaaaatct tcccgatgaa atacaaagcg atgttgcacg gcgaattgct
    37201 acaatggata gaacttctcc cgatgtattg cgtgaagttg agcgagtgct cgaaaaaaaa
    37261 ctttcgacgc tttctagtga agactatact gcagctggtg gtgttgaaag tattgttgaa
    37321 attctaaacc ttgtagatcg ttcttcagaa aaatctatta ttgaatcgct tgaagaagat
    37381 gatccagact tggcagaaga aatcaaaaag cgaatgttcg ttttcgaaga tattgttatg
    37441 ctcgacgacc gtgctataca aaaggttatg cgagaagtcg acactcaaga gcttgctaaa
    37501 gcccttaagg cggttgatac agaagtacaa gataaaatat ttagaaacat gtcaaagcgt
    37561 gctgctgcga tgcttaaaga agatatggag tttatgggac ctgttcgact taaagatgtt
    37621 gaagaagcgc agcaaaaaat tgtttctaca attcgccgtc ttgaagattc tggtgaaatt
    37681 gttattgcac gttctggaga agacgagatt gttgtatagt aaaatattca aaaagagcgc
    37741 atttttgatt tcataaatct gtgaattgag gtaggagaat ggcaaaggca gtttttagac
    37801 caacggaaat tacaaatacc gattccaagg taattcttaa actttctcac aactatgcac
    37861 cagaggaaga agaagaggaa atttctgata tccccgaata tttaggacct actgctgatg
    37921 acttacgacg tgaagcggaa gcatttaaaa aagaatggga agctgaaaaa gaagccctcc
    37981 ttgcaaaaac cgatgaagaa gcagaaaaaa ttataaaaaa tgcagaaaat gctgcttttc
    38041 aagaagtaaa gcgaaaaacc gatgaagcaa atatcattaa ggcggaagct gaactggaag
    38101 cacaaaaaat aattcaagaa gcagagcaaa aagctcttaa aatacaagaa gaagctgaaa
    38161 aaaataggga tagccttgtc aaagatgccc acaaagaagg ctttgataag ggaagtgaag
    38221 agggctatag atctggtcaa aatgaagttg atcgtctcat aggaagactc catcttataa
    38281 ttgagcgtat tatgagtaag cgccaagaaa tacttgatga aactgaacaa caaatagtag
    38341 aacttgtcat tctaatgacg agaaaggttg ttaaaattat ttctgaaagt caaaaaaacg
    38401 ttgtgatgag caatattgta caagcgcttc gaaaggtaaa aggtcgaggt gatgttacac
    38461 ttcgtgttag cttagaagat ttgcaattaa caagcgatca tacaaaagag tttatgaagg
    38521 ctgttgaaaa tattaaaaat attaccgtcg tggaagatac gaccattgaa aaaggcggtt
    38581 gtgtagttga aacagatttt ggtgccatcg atgcaagaat ctccagtcag ctcaatgaac
    38641 ttgaacaaaa aatccttgaa atttcaccca ttaaaacaat tacaaagtca agctcaatca
    38701 atccggagct ataatctatg aaaacgctta tagataaata tatacgtacc gcagatacga
    38761 cagatactat tttatatacg ggctctgtta tttctgtaaa aggaatgctc attgaaagcc
    38821 atggaccaca atccgtgatg ggagaaattt gcaatattct cgtatctgtt cccggagaaa
    38881 agacaagtcg atctattcta gctgaagtag taggtttgca tcatacgact gtgcaactta
    38941 tggcctatga agaaacaaaa ggaatcgaaa taggttgcaa ggtaatagca agcggttctg
    39001 ttttacaagt tggcgttgga gaggcgcttt tgggacgtgt aatcgatgcg cgtggtaaag
    39061 cttgcgacaa taaaggtgaa atttatacaa caagctttta tcctgctctt gcctctgctc
    39121 ctcctgcctt atctagaaag cccatcactg aacgaattgt aacaggagta cgcgctatag
    39181 attctctttt agcggttggg aaggggcaac gactcggtat ttttgctggc tccggtgtgg
    39241 ggaaatcaac cctactcagt atgattgcgc gaaattcaaa cgcagatatc aatgttattg
    39301 cgctgatcgg agagcgagga cgtgaagttt tagactttat acacagagat ttaggggagg
    39361 aaggattaaa acgctcagtt gttatttctg ctacctcaga ccaatccccc atagcccgtc
    39421 ttcgaggagc ctatgttgca acagctgttg ccgaatactt ccgtgaccaa ggtaaagatg
    39481 tgatgctcat gttcgattca gttacgcgat ttgcgcatgc acaaagagaa atagggcttt
    39541 ctgcaggaga accgccagct cagcgtggtt atccgccgag tgtttttgaa ctcttaccga
    39601 agctccttga acgaagcgga acgagcgaaa agggaactat taccgccttt tatactgtac
    39661 tcgtcgacgg agatgacatg gacgagccta ttacagacaa ggttcgcggt accctcgacg
    39721 gacatattgt tttaagtaga cgccttgccc aagcctacca ctatcctgca attgatgtct
    39781 tggcaagtat tagtcgtttg gcaaatcgtg tttcagggcc caagacaaag gaagcttgtg
    39841 gggtacttcg tcgattgctt gctatctatg cggaatcaga agatttaatt tctgttggag
    39901 cctatcaaaa agggacaaac cctgacatcg accgagctat agaaaaacac gcagacatag
    39961 aagcatttct catgcaagaa gaaaatgaac catctactat acaagagacg ataaaacgtc
    40021 ttggtgaaat agctgaaata gctattccaa aagaagaact agaaacactt tgaaaaaatt
    40081 taactttcga ttagaaaaaa tactttctct tcgagatttt gcagaaaaag aggcaaaaat
    40141 cgaactaggt cgtgctatct cccatgcaca aactatacaa aaccacatag aagctcttgc
    40201 aaaggaaaag catgagcaaa ataaagcccg ctccaaaacc tccgctattt atgacttgca
    40261 agctatcgaa gcctatatca atcgattgga cttaaaaaaa gaagagctat ttgctgaact
    40321 tgcgaaggca gagatcgtag tagaagaaaa aagagctctt ttgcaagaag cgatgaaaga
    40381 aagaaaggtg ctcacaaagc ttaaagagaa aaaacaggaa agccatagaa aagaagtgct
    40441 tttggatact gagaaaatac tcgatgatat tgcaaatcgg caaatctagt acttaaagtc
    40501 gcttccacct ataaattctc gtatgagtga ttggccaggg acgagatttg agggtatagg
    40561 taaaaaattg cttaaaaaac gcaacaagcg agacgcttca gaatactcaa cttgatttgg
    40621 attcaccgcg cgaagaagtg gttctgcata cattttaaga acagcaagct cataatcaag
    40681 cgctgtattc agcatcgcat cggcattacc ttgaaagggg taaatatgca gcttctctcc
    40741 tcgttgcaca ctcggccaca tttttatagt gtcaaccgct gttttaccgc gaaactgaga
    40801 atcacgcact attcgtcgaa taagccgatt atcgcttgtt gagatacgat tgtgatcatc
    40861 gagattaagc tgtgtgagtg ctgaaaggta aattttaaac ttaatatcgt tcggtaagag
    40921 cggcgttaac ctatcattta agccatgaat gccttctaaa atgaggatat cttccttgca
    40981 caattgcatt tgctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncctgct
    41101 atgaggataa cacgtatacc ttctctttca tgaatctgat cagcaatatc ggctattgtt
    41161 ttgttttgaa aggtttcaac aatatctata aattcttttt gctttctttc atgaataagc
    41221 gcatttaaag atgcacaaga gctcacattg agctgctttc cccatgtttt atatctattg
    41281 tatacagaaa aaagttgcgg taaatcttca aagtcggaaa tttcatcagg ttttgcggtc
    41341 attgggaagc gaagtaaaaa accaccttgg tagagtttta attcaaaaag ctttaaaata
    41401 cccgtagaaa ctaaaagcgg tgcaaaatag agatcgcaaa agtctcctaa ggtatttata
    41461 gggacttttg ggggacaaat atagttaagt tgttttcttg tttctgtgag attgagcttt
    41521 tcaaataatt ctatagcgtc gttataagat atataggact gttttataat atggtctgca
    41581 tagatgagct tacgcatatg cttttcgaga ttttctaaat ccttttgttg taggacgaaa
    41641 tcggaatcaa aggtataata ataaccataa ccaaggctat gtccaaccaa aagtctttta
    41701 gctggaaaca attcttgtgc agctgcagca agtacaaaac aaagtgttct gcgataaatt
    41761 gcagagcctt cgtgtgtttt taggtaaata ggctttagtg tacaatggat atcaataacc
    41821 tgcgtgagtg aacaggtttc gttatttatt tttaccgcga taatttcatc atcattttcc
    41881 tcaaattctg ataaaatatc ataggcacag attcttttag aaacctttat ttcttttttt
    41941 ccgtcaatac ttatttttat ttctttcata gcctatatta tacatgaaaa aaatactaat
    42001 acaagaaaaa aagacgagat tttgccttaa attataggca taatctgctt tcattgttat
    42061 aatgcaaaaa taaagaggta ttgtatgaaa atagaaattc gtaatccaca agctgcagat
    42121 attgcctatt tatatgatat ttgcttaaaa accgcttcca atggtcagga cgcagcagat
    42181 ttatacactg acccttggct tgtcgggcag tattatgtag cgccttatgt tttttttgat
    42241 ccttcgaatt gttttattgc ctatgacagt gagagtgagt ataaacgtcc gcttggttat
    42301 attgttggaa cagatgatac aagggctttt tacgattggt tttattctga gtgggcgcca
    42361 caattacgaa aagtttatgg aaaaaaaatg cctccgcaat caaaatatga agcctggctt
    42421 ataaacgaaa tatataataa aaataagcag gttgacgatg tccttttaga tagctatgct
    42481 gggcatcttc atattgattt attgcctgtt ttacaagggc aaggtgtggg aagaagcttg
    42541 atcgatcgct tttcaaatgc tttgcgaaaa aagatgtcca atggtataca tcttggcgtt
    42601 tct
//
LOCUS       DAOSSW010000006        40127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_1112_length_40127_cov_7.833824, whole genome shotgun sequence.
ACCESSION   DAOSSW010000006 DAOSSW010000000
VERSION     DAOSSW010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 40127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40127
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1112_length_40127_cov_7.833824"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PKW26_01160"
     CDS             <1..602
                     /locus_tag="PKW26_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOQ92209.1"
                     /translation="EDLLNQEPFDERRACNTAKNHLLNIIGTFPAEWDLVIAPHRKPE
                     KGFIEKRFFHPGLYVYAEDIRSPFNLGSIFRTAEAMGAEKVFISPFCTDPLHPRCQRS
                     AMGCVESIPWERSPLENLPKDLPCFVLETGGTDIEDFIFPKEGIVIIGSEELGVSPEA
                     LSYAQYGRVSIPMTGIKASLNVGVAFGILMYAWTMHLNQ"
     gene            complement(624..2105)
                     /locus_tag="PKW26_01165"
     CDS             complement(624..2105)
                     /locus_tag="PKW26_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOQ92210.1"
                     /translation="MSQINVSKIKKAVEAEIPILITTYTLPHDMELYIREVITFYLKE
                     LHQEHMIEYIVYCVNELVTNAKKANTKRVYFQEKGLDLFNEEEYNEGMEKFKEDTLGN
                     ITHYLKLQKDAGLYIKLILHKKGNTIRIEIRNNSPLTVFEYKRMHDKIARSHEFGSVE
                     EAFVHFIDETEGAGLGLIILTLMLKKVGLSEENFQIASEGEETITRIDLPLNKESEAQ
                     LNVASEEIAKSIDALPFFPENIASIHALMSDPEVEFSEIARLIGNDISLTADLLKLVN
                     SSVFALSQPCNSIVRAVQMVGIKGIKNLLYSVESLKILGEETPGKKMLWSHSQKVAFY
                     SYNIARNFFATKKEIVENAYVCGLLHDMGKVVFETTHPEVMKKFKDIMAYKNIALNTL
                     ERLIAGINHAEVGARIGEKWNFPEMITQAIRYHHNPLVAPESVIPLGVVVYLANMLTH
                     YEESAVDFYQFDTEALKKCNISSKEQLDSIASQLSAAFAKTQK"
     gene            2497..>3044
                     /locus_tag="PKW26_01170"
     CDS             2497..>3044
                     /locus_tag="PKW26_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92211.1"
                     /translation="MQKDFFSLPFKIVSAFLLILSLSLFLLLSIYRPLPNKISNLVTS
                     KTLYYGSHKSGFFERRGLFDAFTAEKIEVFPAENTFTNDKDLQIVVCIPDFCYDIEDT
                     YKTAINIANKGYKVVLFDFFSRDTEGFSVFRQGKNIKNFHEKLYLAFKPEIYEQEKQN
                     LIEQKRKEMLSAMSLIYSEKPEP"
     assembly_gap    3045..3144
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3145..3796
                     /locus_tag="PKW26_01175"
     CDS             <3145..3796
                     /locus_tag="PKW26_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOQ92212.1"
                     /translation="IQKIAYAVDACQASIEKAIEIGADILFVHHGLFWGKEAVLKGLH
                     YNRIASLIQNDLALYAVHIPLDAHPEVGHNASIVKHLGLSNIEGFGSWRSMDIGFKGS
                     FPKAKNIDEVSALLFKGSEKALQTMPFGKTNISSVGVISGGAGGDVWQAVEQNLDLYI
                     TGEISHDLYHFVKENNISVIAGGHYHTETFGLKNLLQKIEKDLHITGVFLDIPTNM"
     gene            3930..5939
                     /locus_tag="PKW26_01180"
     CDS             3930..5939
                     /locus_tag="PKW26_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ92213.1"
                     /translation="MEKLLIIGGVAAGATAAARARRLNSDIEISILEAGSDVSFANCG
                     LPYFLGGEVENRSDLILASPETFKEEYNVTVHLNTEARTIDRKNKTVTAIQNGKKRVF
                     DYDKLILAQGGKPIVPPLPGVDQKNVFTLWTLSDMDKINTFITNKKPKTAVVVGAGFI
                     GLEMVEALVHRGLDVHLVELAPQVMIQLEAEIAGFVTKELLDYGVRLHLNTSLQAIKK
                     DSVSLSDGSKIKADLVLLSVGVRPSLQLAKDAGLAIGETGGLLVDEYLQTNDEAIFAA
                     GDMNEITNTISQKKQRVPLAGPANRQGRIAAENALGGKKRYKGSAVSSIVKIFKAHAG
                     STGLSLKQAKEAGFNADAIVVHKSSHTSYYPGAFRVSLMLIFDKTDGRILGAQAAGRV
                     GVDKRLDVITTAIAGKLKLEELGELDLAYAPPFNSPNGPEQMAAFVAENHRIGFSPSI
                     LAQNLEDWVLAKSPIIFDIRDPISYSRAHLSQTNNLSQGQIQESLDSLPKDSALLVIS
                     EDGQKGHILTRMLLTKGYSNVLNLSGGYISLERQERAKPFEKLRVGLHAIEKKSIQKS
                     SESHEKKASEVNTAVEKTEGPLIIDVRTPMEFKMGAVPGAIHADLDSLEEKIPVITKN
                     DFNREIILYCASGARSSYGVRILKGLGYTNVTNGGGLHTMMSRFA"
     gene            6012..6584
                     /gene="lspA"
                     /locus_tag="PKW26_01185"
     CDS             6012..6584
                     /gene="lspA"
                     /locus_tag="PKW26_01185"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOQ92214.1"
                     /translation="MEEFVQIDFKKKKTSFTKSLPFSLAILLFLLDQGTKLLIEKNIP
                     LNSVGFSLFGDFFRIIHVTNKGIAFSLGSSFQDSARQILFAFLPLVVLGFVVFTYFKN
                     NDFSPFQRWTIAGIIGGGLGNLFDRFFRPNGVVDFIDVKFYGIFGFERWPTFNIADMS
                     VLICGILLLISFFLTILKEKKKQERNPNDK"
     gene            6574..6849
                     /locus_tag="PKW26_01190"
     CDS             6574..6849
                     /locus_tag="PKW26_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide processing enzyme"
                     /protein_id="HOQ92215.1"
                     /translation="MTNKQRTLIFIAVGTIISIILTLIILILLILVGSLLLKERIANF
                     LPLVLMIAIFLSLFVYQKLTVFVVQKWNLEDKLDPLFKSKKRRKPRD"
     gene            complement(6875..8548)
                     /locus_tag="PKW26_01195"
     CDS             complement(6875..8548)
                     /locus_tag="PKW26_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92216.1"
                     /translation="MNDQLSPEIEALLAETAKKPSFGLSKIDEDESLGFQKENSWGSP
                     KIDEKKDSHLEVDLSLTGFKPITEFFSSDLNPVFGNPNYYKTALAGETEAAQRFHTVL
                     SKYLTSKDPKDRSVFRQQLNTLYWELVRSIAPKTVSLTIEEEKKMLLRFGLVLPSLLT
                     NEQKDTFASVFPKNNTGEPVFYVDEWFRDIGNGKMAPSATDEARPTKKGSSGGNAEAQ
                     RLRQLQAKNEGKLQASERMLTAKERERSMLEIELKSKVDKFCEHPSYGNDIHKGFLTE
                     AQKKLVPSIGEDLKSLLKIDREIQSYLEEYTDAKGVAESLEVKLQAMPEDCEVNTSDI
                     LQEIDTVRQMAKMTVGRQGNHFPLLTREFFHCMPRQTGYRENVIDLMAKIESIDPGAF
                     CRVHKNNQNRIVPYVLLVPTYGDSGMCWEPFDRYNRVTSRGRIVVPMYPRNLEIALLT
                     AVADLRWQVAKEKASYYWMEEGLTGQYYQWFYTQKLKGDVKEYFINDYILWMTKESEG
                     VQRLEKEVRGVFWRHMPFSQEIKENLRTRSLVYQELYQRDLNRAMSDGY"
     gene            8671..9624
                     /locus_tag="PKW26_01200"
     CDS             8671..9624
                     /locus_tag="PKW26_01200"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ92217.1"
                     /translation="MNRFDVIIIGKGPAGISAGLYLKRSRYEVLIIGKDFGALDKAEK
                     IENYYGLENPISGKELSETGIKQAEQLGIDIFTCEVTSVEATFEEPYRFKISATQGEK
                     KQEFLSKSLLIATGKQRKTLRIPGFDDFNGKGISFCATCDGFFYKNKTVGVLGAGSYA
                     MSELSYLYNLSQDILLFTNGDPQIEKTLLDSPLPEGVRLITDKLSQIYGTEYQDNVSV
                     LAGVETKDSEGNIQRHDLQGLFVALGTAGASEFATKLGLATGEGTNAGTILTDDGMRT
                     NVPGVYAAGDATGGFLQIVKAASDGAIAAKSIGSDLKELSR"
     gene            9725..11713
                     /gene="htpG"
                     /locus_tag="PKW26_01205"
     CDS             9725..11713
                     /gene="htpG"
                     /locus_tag="PKW26_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOQ92218.1"
                     /translation="MAKYQFQTEVSQLLKLIIHSLYSNKDIFLREIISNASDALDKLK
                     YLNLSDENYKSIVFDPRIDIKFDEKEKTITVQDSGIGMDEEDLNANLGTIARSGTKAF
                     LENLEKEAKKDSALIGQFGVGFYSAYMTSKKIDVITKKAATEKILLWSSDGTESYEIE
                     EVAKDSEKAKNLGFDKDGSNGTAIIMYLTDEDASYASRWKIEELIKKYNDHIAFPIFL
                     HFEQKQYDDKGKETSSEAKVEQINSASALWKRSKSDLKTEDYNEFYKAIAHDSNDPLL
                     TIHTHAEGTQEYTTLFYVPETAPFDMYQADYKSGLKLYVKRVFITDDDRELLPSYLRF
                     VRGIIDSEDLPLNVSREILQQNRILSSIQTASVKKLLSEFKKHAEASDKALEKKAALS
                     EGESLSDEEQKAIDRWNTFISQYNRPLKEGLYSDYANREELSEIIRFKSTAEEGTGEN
                     AWTSFAAYVQRMKVDQKAIYYITGGDEKNLRSSPLLEAYKAKGIEVLIMSDDIDDIVI
                     PSLMKYKDFDLKAANRAGSDEELGVDKKEAEKKEKEFKPIVEKIKTALGDKVKDVHLS
                     KRLSDSPSCIVVDENDPSLQMERMMKAMGQAGFSEVKPILEVNGDHALVAQLKDTEDA
                     AFIENLSNVLLDQALLLSGAELKDPADFVKRLNALLAK"
     gene            complement(11824..12417)
                     /locus_tag="PKW26_01210"
     CDS             complement(11824..12417)
                     /locus_tag="PKW26_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOQ92219.1"
                     /translation="MEIYDAMQERRSIYNLGKNKIISEDRVQEIVSFALTHVPSAFNS
                     QSARVLLLFGEESSKFWSLTKDVLEKIVPADAFEKTREKLDGFDSGFGTLLFFEDQNV
                     VNKLAKDFSLYAEHFPLWSLQSSGMLQYAIWMSLASEGIGASLQHYNPLVDEAVRKTW
                     QIPESWKLLSQMPFGSVEAKADEKKTLPLDERLWVRK"
     gene            complement(12596..13162)
                     /locus_tag="PKW26_01215"
     CDS             complement(12596..13162)
                     /locus_tag="PKW26_01215"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOQ92220.1"
                     /translation="MHFVALDFETGDYAPNSAVSIGLVKYTNGKKTDEYYSLICPPKL
                     YIRPDFTEIHGLRVKDVEDAPLFSQIWAAVKEFIGDFPVIAHNAGFDMGVLCALIAHY
                     KLDLPSIQYYDSVFIARKTWPELACHKLTFLGQNFGIQYEAHNALADSDTCAQIIVKA
                     AEKQGAKKLADLSPRYYQLKKLWERKFG"
     gene            complement(13212..13346)
                     /locus_tag="PKW26_01220"
     CDS             complement(13212..13346)
                     /locus_tag="PKW26_01220"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG-related small transporter subunit"
                     /protein_id="HOQ92221.1"
                     /translation="MDFLALQKSFLLMGIGMAVLFVFMGILILVVDLTVKVFRKKDIQ
                     "
     gene            complement(13348..14487)
                     /locus_tag="PKW26_01225"
     CDS             complement(13348..14487)
                     /locus_tag="PKW26_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOQ92222.1"
                     /translation="MLENIFAFFLEGILAIGYEQVLMYLIGIVLIWLAVAKDYEPMLL
                     LPIGFGAILVNLPLEVVWSHDGAPGALKILFEAGVLTELFPLLIFIGVGAMIDFAPLF
                     QRPWLMLFGAAAHLGIFATISLAVALGFPLEEAACIGVIGAADGPTTIFITTKLAKDL
                     LGPIMVVAYSYMSLVPIIQPPVIKALTTKNERLIRMTAVDHSIPRVVFVIFPIAVTLI
                     CSILIPIAAPLVGFLMFGNLIRESGVLEKLSLTAQNELSNIVTILLGITVGASMRADS
                     FLRPETLLIMALGLFAFVFNTAGGVLFAKFINLFLKKKINPMIGACGISAFPMSSRVI
                     QKMALKDDPTNIILMHAVSANVSGQLGSVVAGGLVLALIPILIGG"
     assembly_gap    14648..14747
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14748..15891)
                     /locus_tag="PKW26_01230"
     CDS             complement(<14748..15891)
                     /locus_tag="PKW26_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOQ92223.1"
                     /translation="MMYFLARIIVFLLCIIACFMLIPLAFAIYFSEVDVYLSFIIPIA
                     FCFLLGLLAFFFKAKKKQRLPMRLGFAVVALSWLFSCLLGSIPFMISGYIPSFFDAFF
                     ESVSGFTTTGASILSSVEALPFSLNIWRAEMHWLGGMGIVAITVALMPLLGIGGFQLI
                     KAETTGPDKGKITPKIRETAKKLWIFYVILTFLQVILLLFAKMSFTEAVFHSFATLGT
                     GGFSMKNSGLASYSASAQWICTVFMILAGVNFSLYFLLWNKKRQELLKNTECKVYFSI
                     IFIASFFVFLSIYPLYGKIEEAMRHAFFQVASILTTTGFATRDFTLWPGAAQSLLFFL
                     MFIGGSSGSTAGGIKVVRWTVLFKQATNEIKKMLHPHGVYTIRLNQK"
     gene            complement(15888..17318)
                     /gene="trkA"
                     /locus_tag="PKW26_01235"
     CDS             complement(15888..17318)
                     /gene="trkA"
                     /locus_tag="PKW26_01235"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOQ92224.1"
                     /translation="MKVIIIGAGFTGTELAKRLISEGQDVVLIDKDEETIRHASNRLD
                     CMLLQANGNNLNVLEEVGIETADFLVALTESDELNMISCSLVDSVYPQVVKIARVRNS
                     DYYNDIGIQSKKNAEKNRRPPYGIDFMIQPDLEAAEAIVNAIKWDTVSESIDFEGSDY
                     QIASVLVKKDSALDGLAIQNLKSITKKQFLAVYIENEKTAYIPNGEAIFQAGDRIGFL
                     TKDEDVESLLQLAGEEKKTIRKIAILGAGRIGNRVAGRLMQAKKKRFFSHVFSVPQKL
                     RKEFAIIEKDGQLAKNAAELFPEATVYKADISDEGFIEEEGLQNYDVLIASTHNHELN
                     IVTAAYLKSIGDIKTVCLVTNAQYAKIANGIGIDVAIPIKDVVIDAIMSHLRGKSISR
                     IHTLFEGEMEIAELSVLKDSPFYGKNLKDLSAEAAFLVLLIKKEKELLYHIPTGDTKL
                     FAQDSLIVLYDREKNKEFMQGFGVKQ"
     gene            complement(17326..17949)
                     /locus_tag="PKW26_01240"
     CDS             complement(17326..17949)
                     /locus_tag="PKW26_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOQ92225.1"
                     /translation="MKTNNQEMIRALAVTAALGALTIVLTITRLGFIPWFSGAAITIL
                     HVPVIIATLLGGLWAGLGVGLVFGLSSLIQAAVGPTGVLDPFFVNPIVSVLPRMLIAL
                     ITFFVFKALQSMNKNNSPFLKQGFVGVAAFLGSLTNTVFVIGALYATHYTDLVAITEG
                     AGYIAFLIMLLPQALLEAGASVVLSIAVVSLYDVSGRKKSKLEQEIE"
     gene            complement(17997..19256)
                     /locus_tag="PKW26_01245"
     CDS             complement(17997..19256)
                     /locus_tag="PKW26_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOQ92226.1"
                     /translation="MFYNFIQEKIAIFQVSEEWQSIIIVLISYFVLFLLTFIFKQILS
                     LFNKLVVSKAIKKSKITWDDIIWDLGIFKQVILVGSFIFILTLYPVFILDEFLAASFI
                     KRLIVAFFYFTAAKTIALFLDAINEIYKQFRPELAKRKPIKGYLQMVKIFLYIVAVIL
                     IITSLIDVSPLGLLSGLGALSAVLMLVFKDPIMGFVSSLQLTGNDLVRIGDWVEIPKY
                     GADGAVLDINLQTIRVQNWDMSITSVPVYALVSDSFKNWRGMSESAGRRVKKSIYIDM
                     KTVRFLSEEELDRLEKLPRLKEYLQEKRSEIKEYNAKLNLSSEDEITPRKLTNLGTFR
                     AYIVEYLKEHPLVSKTLTFMVRYLDPTSKGIPVELYFFSANKNWVPYEGLQSDVLDHV
                     LSVMHFFDLAVFQDLTGEDFKQGKKNS"
     gene            19323..20132
                     /locus_tag="PKW26_01250"
     CDS             19323..20132
                     /locus_tag="PKW26_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92227.1"
                     /translation="MYNSKIGIATSFFADQNFDSSLTLALAHEENISWVQLFLSPYTE
                     NPHEIFTLQGLATQHGTKIICHSPLLLNLDAKDGIHAANLSLFFPQGQEKFSVFHFDE
                     NETIENALSVIEFYSTNGFTICLENFYQDKSKDAFIQNFHDYLALIEAAQEKKYKIIP
                     VLDFPRLFIEPITEAVDALAYTELLLDKLAKTTKNLILHLIDFSKSSQKREEWLPFMA
                     GIMPYEKIFDLLKKYNFTIISAILEYENKEIGRQSFQALSESLDKLVSNKS"
     gene            20186..21184
                     /locus_tag="PKW26_01255"
     CDS             20186..21184
                     /locus_tag="PKW26_01255"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOQ92228.1"
                     /translation="MIVFFLIGILAISLFSFSLSAGFILLNAQIKPYDTSEKLKEEES
                     AMPGMPTELTAHVRDPKIMTSLLAAKNRWYENPIEKLEQKSFDLKPIYADFWEYSKNP
                     KATAILIHGHMDSARGMAYLAEEYHKRGFSVLSIDMRGHGRSKLKSSFGYKDAKDILL
                     WIAFLQKKYNIDHKIIVHGVSMGSSSVLRLLSLKKAPANIALAVVDSSFASFQTQLKS
                     QIDNLLPNNFFQNFVKKLLLFGLSCSNFLVQGFFFFQHAPIQYLKKRNKSRSKSIPIL
                     FFHGLKDSMVPVKNCEILFALTNEPKKQILIDDALHIGAYFYAPEKYMQEIENLFH"
     gene            21388..22107
                     /locus_tag="PKW26_01260"
     CDS             21388..22107
                     /locus_tag="PKW26_01260"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HOQ92229.1"
                     /translation="MTLFGVIGAMEVEIALLLQKMEETAAVKTKQIASLTFYEGEIGK
                     AALVLVKSGIGTVNAALCAQILISEFKVSHLINTGIAGALSKNLGVLDVVLSTDAVYH
                     DFRVTGFGYKPCIIPGMEKSVFSADSKLIEKAKNAYTSAHFVHNLYTGRIATGNLFVD
                     QTNEKARIISDVSDLDGISPIAVEMEGAAIAHVAYVNQIPFLIIRSISDTADESIEKG
                     EYKEEEAAKISSFIVQSILED"
     gene            22110..22586
                     /locus_tag="PKW26_01265"
     CDS             22110..22586
                     /locus_tag="PKW26_01265"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HOQ92230.1"
                     /translation="MKKKYNVESFNLDHNAVTAPYVRLAASYTGPNGDIVTKFDIRFT
                     QPNKAFLPTGAMHTIEHLVAEYIRDEVSGVIDFSPMGCRTGFYFTLFGNKTEEEVSAH
                     FKAVFQKLLDWPDTEKLPGYDPKECGNYLDHNLAEAKAYTKKWLEGIEKYGFSCYK"
     gene            22609..24039
                     /locus_tag="PKW26_01270"
     CDS             22609..24039
                     /locus_tag="PKW26_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOQ92231.1"
                     /translation="MILIFSLLIIVSMISTKLSSKMGMPLLVGFILVGIIAGSDVLNL
                     LYFDNALMAKQITDILLIFIIFEGGFRVTRKNFHKTAAPSLTLATLGVMLTALALGLF
                     IYFVLKLDFLTSFLIASIISSTDAAAVFMVTKENPINPKLATTINVESAANDPMAILL
                     TVTFIQLLTEGIKSPLTIIISLTWQFLGGVAIGYLVHIVTIFVFNKFNSDNRGNYNVL
                     IIGQILFCYGLAVLCKANGIIAVFFMGYWLGNANYAAKQGVSNFLESIASMCNVGLFL
                     ILGLLSFPSRFIHIWKEGVIVVLVLIFIARPIAVFLCTIPFKYNFKEKLFIIWGGIKG
                     AVPIVLATYPAAYGLDENGYIFDIIFFAVFISCLLQGSTLGLLSKAFNFTIPKRQTTP
                     YVVELHSTQETDVEMFEIHIDDDAQSINKKISKLDLGQDVLISSIIRDGKIVLPKGNV
                     CIKAQDILFILTSTEKIDEINAILNN"
     gene            24064..24954
                     /locus_tag="PKW26_01275"
     CDS             24064..24954
                     /locus_tag="PKW26_01275"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOQ92232.1"
                     /translation="MQKIKALNKNEIEKHYQAFSPLEIKPKIHIFPELDSTNSYAKQF
                     LKENPLESHGSIIIAEKQSAGRGRLGRSFTSNTDEGLYISFILNTDNLPVPLITPYVS
                     LALVRSIKSIWNIDAKIKWVNDVFIGTKKIAGILTEASFSSQEKGLSQVIVGIGINIF
                     QKESCFPVELQAIAGSIYQALLDRASVSIDSENNKTEVDEDFMELFFASKNIFTAQLI
                     HNVFRILTQDIHKISENIAEYKAYSNILGKEVLVLQGDKSFLAKAVDINEEGHLIIED
                     ALGKKQEILSGEVSIRLPTQ"
     gene            complement(24944..25648)
                     /locus_tag="PKW26_01280"
     CDS             complement(24944..25648)
                     /locus_tag="PKW26_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOQ92233.1"
                     /translation="MMTKKPAHLWLKEEIQKIEKNPCFTETKKPSLLIHACCGPCSSY
                     VLEYLEKTFAISLYFYNPNIYPQAEYERRKDELLTFIEKYNNSIQVITEKTYQASDFY
                     EATGVKTETDLQKEGERGERCRRCYAFRIKKAFIYAREHGFDYITTTLSISPHKDAEK
                     INAIGRALEEELLESEQLEDGAIAKKAGNKPRFLYADFKKNKGFLRSLELSKEFGLYR
                     QDYCGCLYSIRDGIIE"
     gene            complement(25645..26955)
                     /gene="argA"
                     /locus_tag="PKW26_01285"
     CDS             complement(25645..26955)
                     /gene="argA"
                     /locus_tag="PKW26_01285"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HOQ92234.1"
                     /translation="METPKILENAQNIRSVIRYLKRFKNTTFVIHLPDSILESSVFLN
                     HISDIALIHNAGIRIVLVAGAKKRINCLLESAGILWQEKDGLRVSGENAMPHIKMASF
                     DVANSIMTALAAEKISAVIGNWVKARGKGIIDGFDYESCGEIDQIDLPSIHSVLENNF
                     IPIFPCLGWSSSGRPYNISSIDLAKEIAIQLKSEKLFFLNDYELEEIFLEKKAHENES
                     INALSVQNLEAILKENRAIPIHAARLLNLALEACKKGLERVHLIDQRIDGVILNELFS
                     ELGSGLMIYDDNYGAIRSMDLEDVADVLSLMRPFVKKGILLERSEVQLKELHGDFVVY
                     EIDGAIRACAALHVYGEEQGEIAAVVVDERYNKAGIGIKLIDFLKKKARGLGIASLFV
                     LTTQTADWFEKQGFKPDILESIPLAKQEKIDKNRKSKVFRLELV"
     gene            complement(27012..27707)
                     /locus_tag="PKW26_01290"
     CDS             complement(27012..27707)
                     /locus_tag="PKW26_01290"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOQ92235.1"
                     /translation="MLELLQFKKSLLFSQLRELEQIYEAIEKDEEEWKKHSPTQCPSG
                     CGQCCIAFEPDLLEIEADYLALWLIQNDREKAEAIADQTYIWPRFLEYPQGCFLFDPN
                     DDYHCTVYEGRALICRLFGYTSDTGRDGKIRWKPCKYIPEEAFKAGFVRKQYTEEELR
                     ESLVALPPVMSHYLRKTLSIQPDNAGRTTAMRDILPHAIQKMLFLLKYIELESSVDNN
                     PDDTPTPTSPIAA"
     gene            complement(27774..28616)
                     /locus_tag="PKW26_01295"
     CDS             complement(27774..28616)
                     /locus_tag="PKW26_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOQ92236.1"
                     /translation="MKCTCKSLRQRGLFLFILGFYFIAIIQPVLAKEEESLLFDISLK
                     KESLIIASSLAITVFPLLMPKASYEKEAPLQDISRLPFFDAWSANPYSKNFDLAGDFF
                     QYTSLLLPAVLLCTEKTNWLTIGVMYAESIFLSYGLKNLGKTLFPRYRPYTYFEGAPF
                     SDDTDFKKSFPSGHTTMAFTGASFLSSVFARYYPESQWKWPLILGSFSFATTTAALRI
                     ASGNHFFTDVLSGAILGSFSGFIIPYLHRIETNINRKNNGAEKPVLVQLNISSFSSSI
                     RFLF"
     gene            complement(28634..29596)
                     /locus_tag="PKW26_01300"
     CDS             complement(28634..29596)
                     /locus_tag="PKW26_01300"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ92237.1"
                     /translation="MDEGLKAIEGKDGVFAAIDTTRGLIVAELFYEKTPLTVTNFVGL
                     AEGSLKATGGKNFYDGLKFHRVIADFMIQGGDPKGNGTGGPGYKFADEFDDSLKHTGP
                     GILSMANSGANTNGSQFFITHVATPWLDGKHTVFGKVLHGQKVVDTIKQGDEIKSIKI
                     IRQGEKAKAFTATQDDFDRLSVEIVKQNTAKLEKAMAETIGLIEKKFVGFEKTAEGIY
                     FGIKSEGSGVKCGANKEVAVHYTGYLLNDSVFDSSEGRAPLEFVAGAGQMIPGFDVMV
                     QDMRLGEKRTIVLPPSMAYGSAGAAGVIPGNAYIVFDVELTKIK"
     gene            complement(29666..30472)
                     /locus_tag="PKW26_01305"
     CDS             complement(29666..30472)
                     /locus_tag="PKW26_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92238.1"
                     /translation="MKKSILFLFILSFWFVGEINAEPKKEGFRFLLDDETYAELIKNE
                     SLVSISYRKKEKLKSLAPKATIIDEAKKKWHESHDIDPLIVVEGLFLYTKPKNEKNFI
                     SHTDEIAKTASILTAFSKMKGMEYYSERKKRKEILYEDCFVIESYENQERQEDPKPSS
                     IDDLQILVRQTDTTFGSYVMDVAYFQKNAEIAMYMKNLDPLKYLLFKAIQEDNLHLFL
                     YTHTFEEHILVYFLVQATYLDVAFLNETIEKSLKNRVDAIYEWFKIQYEG"
     gene            complement(30475..31185)
                     /gene="jag"
                     /locus_tag="PKW26_01310"
     CDS             complement(30475..31185)
                     /gene="jag"
                     /locus_tag="PKW26_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOQ92239.1"
                     /translation="MIFEFEGKTEKEAIELAVQELGLEREEFDVEIMETQKNSLFKKG
                     FVKIRVHTDGKKIVSDISSDIPVEAAKKTQKKVFGNPLPQDDFEKEIVTFITTVVTKM
                     GYDISVDVMHREEKKIELRLESSSSAVIIGKKGRNLDALQLLTNVYAGKLGRSDERII
                     LDIENYRIRREESLVRLAYTTADRVRTSRNSFLLDPMNPFERRIVHTTLNDISDIETE
                     SEGEGLFKQVRVKYKGFF"
     gene            complement(31250..33040)
                     /gene="yidC"
                     /locus_tag="PKW26_01315"
     CDS             complement(31250..33040)
                     /gene="yidC"
                     /locus_tag="PKW26_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOQ92240.1"
                     /translation="MEKNTILAIVLSSLVLIGFMFLQTYLNPPVDYPVEEVVRDLEDR
                     PFIANEENAQNNLEKMVHLEDFAEEVSISEEKFLIETNNAKITLSNRGGDIVDFELKN
                     HLDGDKGVQMADSISEINRAFSLSFGSYNSKIIDDLFQVNKINDYTYGFYKKFRLAKA
                     DGSFDTFTLIKQYTFHPDDYLFQLDVIIDGLENSNSAFSELNLNGKAYTLRTSPQIGP
                     YYDKKKDRYENRTFMSYTKDKRKKQLLGDNATKEYSNPYTWTGIAGKYFTILVHPQNP
                     ETMGPSLYSTKIEKNDYTNAQVFLTRNAISQKNNKDTYYIYVGPRTDKDLKKYNLLSE
                     NNWGLSNIRFDDSLQSSGILSWLEIFMKAIMQVFYKIIPNWGVSIILMTILLKVALFP
                     LTKKSSVSTLKMQEIQPLMTEIQEKYKGKPEKLNIELAKLYKETGYNPMMGCLPLLIQ
                     FPLIIAMFNLFNNYFEFRGAMFIPGWIPDLSQGDSLYVLGFSLPFLGNHLRLLPVIYV
                     ISQLLFGKITQSTAAAGANATQMKIMTMGMPLFFFFIFYNAPAGLLLYWTISNLLQLV
                     QQLAINKMMKEKKAEIEGNIIPAFSPKKKK"
     gene            complement(33056..33280)
                     /gene="yidD"
                     /locus_tag="PKW26_01320"
     CDS             complement(33056..33280)
                     /gene="yidD"
                     /locus_tag="PKW26_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOQ92241.1"
                     /translation="MLKKICSRIFIFLIRFYQKCISPLFPATCRFQPTCSNYTLQAIK
                     KHGPFKGLFLGIKRISRCHPFHEGGYDPVP"
     gene            complement(33274..33621)
                     /gene="rnpA"
                     /locus_tag="PKW26_01325"
     CDS             complement(33274..33621)
                     /gene="rnpA"
                     /locus_tag="PKW26_01325"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOQ92242.1"
                     /translation="MSKSRGFTKKERVKQKKEIQNLFKTGKRMSVRGAKLFFLPSDLA
                     FNRVAFALPRGYGTAVQRNFAKRISREVYRNLKGELARSYDLLFLFYPGNDTISIREN
                     QIRLLCKKAGLLC"
     gene            complement(33644..33799)
                     /gene="rpmH"
                     /locus_tag="PKW26_01330"
     CDS             complement(33644..33799)
                     /gene="rpmH"
                     /locus_tag="PKW26_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011772387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOQ92243.1"
                     /translation="MKRTYQPSKVKRNRKFGFRARMKTRGGKLILKRRRAKGRYKLSV
                     SDEKKPY"
     gene            complement(33895..34401)
                     /locus_tag="PKW26_01335"
     CDS             complement(33895..34401)
                     /locus_tag="PKW26_01335"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOQ92244.1"
                     /translation="MKDDAISSISTILAKYMDGVDSEMVENAVSIRNVWKDIICSIRG
                     PKKTGFEENIYLGEKLYSHTKIIDLVNKVLLVEVDHSGWIQTLQFYNSYILRGLKKKI
                     PALEITSIAFRLKGSDFGIKNIPEAPKKEEEREEIQKEIQIKEDLPEDLKEKLEKLKN
                     ISKKNKRA"
     gene            complement(34391..35467)
                     /gene="recF"
                     /locus_tag="PKW26_01340"
     CDS             complement(34391..35467)
                     /gene="recF"
                     /locus_tag="PKW26_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706522.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOQ92245.1"
                     /translation="MPFLSLSFVDYRNIENASIDLLSKEVFFKGDNGQGKTNLLEALY
                     ICSYGSSFRTKNDVEIIKKPHSAYSIKALYKDSHEITHSIVIKFENGKKHILKNGKKI
                     TDRKELINAIPCIVFCHEDLDFVTGEPEKRRFFLDQSISLHNNIYVDSLRKYKKILRS
                     RNIVLKEQNFDLLPVFTENLIQIGMEIRKKREDISLFFNRDLSSLYEEISGISGLSLS
                     YHSSWKKMNKDEIEVHLKAKEGIEKNLGTSVSGPHRDRIIFIKDGENFVLTASTGQKR
                     LLSVLLRILQAKYIFEVNHKKPILLMDDVLLELDPGKREKIMHLLPEYEQLFATFLPG
                     EPYEKYVKSKTKVYTIQKGHWDER"
     gene            complement(35474..36577)
                     /gene="dnaN"
                     /locus_tag="PKW26_01345"
     CDS             complement(35474..36577)
                     /gene="dnaN"
                     /locus_tag="PKW26_01345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOQ92246.1"
                     /translation="MKFTFDKDTMLKEISIAQEIIATKNALSILSNVLFIAENNTLTI
                     KATDIKVNFETKIPIEIETEGKTTIFCDKLMSILTSLPSGEVEFIQDETKITIKPIAK
                     KVKFQLKSMASDKFPEFINTDEVNFIEVPVQDLKDMITQTIFAVSDDETRYFMNGVYF
                     DKKDDALILVATDGRRLSYISKALYQGVQEFSPAIVPPKILNIILKRAPNEGSISLAF
                     VDNMIIVKFGNYIFTSVLIEGQFPNYERVIPEKQDFTFEVDKKDLGDALKRVGLLVEQ
                     KSHRVYFALSDGLLTISSQESDIGSAKEEIPCKYSGEDITMAFNYIYIEEPMKVINTE
                     RIRFDFTSSMKAVTVKSEPEKDFFHIVMPMQME"
     gene            complement(36802..38193)
                     /gene="dnaA"
                     /locus_tag="PKW26_01350"
     CDS             complement(36802..38193)
                     /gene="dnaA"
                     /locus_tag="PKW26_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOQ92247.1"
                     /translation="MDKWNYSIFFQEALSQIKESYIQEGKAKEFDMWFSFEYHDSTEN
                     SITIMVPSNFFKDQLISRGYIALLEQKLEELSGKQITIEFFIQAKKEENIQSHNLTND
                     IKIEKKEIVQKPVQNSKGKHPQLREDYIFDNFIIGDNNTFAFNAASAISKNPGKAYNP
                     LLIYGGVGLGKTHIMQAIGNYTHQNTDLKTIYLTAESFTNEFIQALNDRTIPKFKNKY
                     RNADVLLIDDIHFLQNKTETQEELFHTFNVLHDAFKQMVFTCDRPLSEIRNLSDRLRS
                     RFERGLTVDLSPPQYETRRAIIVKKLELMNKKISDDIIDLIAENVVSNVRDLEAAITK
                     IIAYTELIGKTITLEIAKQQLRDAFTSPRQGNISIEKIQKIVADHYNLSFTDLKARKR
                     TKNIATARQIAMYIAREITEFSTTEVGAEFGGRDHTTVMHACQKIEELVKTDTHLQSN
                     IHLLINTIKDAKK"
     gene            38411..>40127
                     /locus_tag="PKW26_01355"
     CDS             38411..>40127
                     /locus_tag="PKW26_01355"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HOQ92248.1"
                     /translation="MNPSYSASNIQVLKGLEAVRKRPGMYIGSTGPDGLHHLVYEVVD
                     NSIDEAMAGHCDRILIVLEKNDICRIEDNGRGIPVDIHPTEGVSALELVLTRLHAGGK
                     FDKGSYKVSGGLHGVGVACVNALSSWLEATICKDGIQYHQRYEVGVPLGPVKEVGKTD
                     KSGTIIRWKADPTVFEETQTYNFEVLASRLRELAFLNSGISIVFRDERLTTPKEQVFS
                     FEGGISSFVSYLNENKICIHQTPVYISGERDEIVVEIALQYNDSFSEILFSYVNDINT
                     KEGGTHLDGFKTSLTRVLNDFLKKNPKIQKKMDKEDRFSGDDVREGLTAVLSVKVPEP
                     QFEGQTKTKLGNTEVRGIVDSFATEQLTLFFEQNPSVIEKILEKAVLAAKARIAAFNA
                     RQATRRKNALEGGGLPGKLWDCIEKDPAKTEVYIVEGDSAGGAAKKGRDAKTQAILPL
                     WGKMLNVEKTRIEKVINNEKLQPVIQTLGAGIGQNFDTSKLRYHKIVIMADADVDGSH
                     IRTLLLTFFYRYMTALIEEGFVYLAMPPLYKLSYNKEERYVYNDEEREKEKLEIMEKF
                     NVKDED"
BASE COUNT        12655 a         7676 c         6857 g        12739 t
ORIGIN      
        1 tagaagactt actaaatcaa gaaccttttg acgaaaggcg agcctgtaat acagcaaaaa
       61 atcatttact gaacattatt gggacctttc ccgcagaatg ggacctcgtc atagctccac
      121 accgaaagcc agaaaaaggc tttattgaaa aacgcttttt tcatcctggg ctctatgtct
      181 acgcagaaga tattcgctct ccttttaacc ttggctcgat ttttcgaaca gcagaagcaa
      241 tgggcgctga aaaagtattt atttcacctt tttgtaccga cccacttcat ccgcgatgcc
      301 aacgctctgc aatgggctgc gttgaaagta tcccttggga acgctctccc ttggaaaatc
      361 tacccaaaga cctcccttgt tttgtcttag aaacaggtgg aacagatata gaagatttta
      421 tttttcctaa agagggaatt gtcattatag gttcagaaga actcggcgta agccctgaag
      481 ccctcagtta tgcacaatac gggcgagtaa gcatccctat gacaggaata aaggcttcgt
      541 taaatgtcgg agtcgccttt ggcatcctca tgtatgcatg gactatgcat ttaaatcaat
      601 agatgtagta aatcgcatat aatctatttt tgtgtttttg caaaagcagc acttaattga
      661 ctcgcgatag aatctagttg ctcttttgag ctaatattgc attttttaag tgcttcagta
      721 tcgaactgat aaaaatcgac cgcagactct tcataatgcg ttaacatatt tgcaagatat
      781 acaacaacac ccaaaggaat aacagattct ggtgcaacaa ggggattatg atgatagcga
      841 attgcttgtg ttatcatttc tgggaaattc catttttctc ctatgcgcgc cccgacttct
      901 gcatgattta taccagcgat caatcgctca agtgtattta aagcaatgtt tttatacgcc
      961 ataatatctt taaatttttt catcacttct ggatgtgttg tttcaaaaac aacctttccc
     1021 atatcgtgta aaagcccaca aacataggca ttttctacga tctctttttt tgttgcaaaa
     1081 aaatttctcg ctatattata tgaataaaaa gcaacctttt gtgaatgtga ccataacatt
     1141 ttttttcctg gcgtttcttc accaagaatt tttaaagact ccacagagta caacaagttt
     1201 tttataccct tgatgccaac catctgcacc gctctaacaa tactattgca gggttgcgac
     1261 aaagcaaaaa cagatgaatt tacaagtttt agtaagtcag ctgttaaaga aatatcgtta
     1321 cctattagac gtgctatttc agaaaactct acttctggat ctgacattaa ggcatgtata
     1381 gaagcaatat tttcaggaaa aaagggaagg gcatctatgc ttttcgctat ttcttcagat
     1441 gcaacattaa gctgcgcctc tgactcttta ttcaagggca ggtctattcg tgtgatagtt
     1501 tcctctccct cgctcgcgat ttgaaagttt tcctccgaca agccaacttt tttaagcata
     1561 agggtcagta taattaaacc aagcccagct ccttctgttt cgtcaataaa atgcacaaaa
     1621 gcttcttcta cagagccaaa ttcatgcgaa cgagctattt tatcatgcat tcttttatat
     1681 tcaaaaacag tgagaggaga gttatttcga atttcaattc ttattgtatt gcctttttta
     1741 tgcaaaataa gttttatata caagccagca tctttttgta attttaaata atgtgttata
     1801 tttccaagag tatcttcttt aaatttttcc attccctcgt tatattcttc ttcgttaaat
     1861 aaatctaaac ctttttcttg aaaataaact cgttttgtgt ttgctttttt tgcatttgtt
     1921 actaattcat tcacacaata cacaatatat tctatcatgt gttcttgatg caattccttc
     1981 aaataaaaag taatcacttc cctaatatat aattccatat catgtggaag cgtatacgta
     2041 gtaataagaa ttggtatctc tgcttcaact gcttttttta ttttcgaaac atttatttgg
     2101 ctcattttgt actccgacat aaataaacaa ccttcttgca aaattttatt aataaagtat
     2161 atcatagaag ataaaaaaaa agaagttcgt tttttataaa aattcttgct ctgtgatagc
     2221 ttttagggta ttataagcca tatgacagac atttttttcg ctcaaatcct tatactcttt
     2281 ctacaatgtg ttacgcttta tcggcctttt tcaaaacatt ttatccaaac agattaccac
     2341 aaagatgttc tttcggttct tccccttatt tctcttgtaa ttatagtttt gtcttttttt
     2401 gcattttcac tgcaaataag cttgatgttt ttgtttttat taggtctttt tacttttttc
     2461 ctgaacctcc caaggactct ttcttttata ggaggcatgc aaaaagattt tttctctcta
     2521 ccttttaaaa tagtatcagc atttttattg atactttcac tatctctgtt tttattgtta
     2581 agtatttatc gacccttgcc gaacaaaata tctaatcttg tcacaagcaa aaccttgtac
     2641 tacggctcgc ataaatctgg tttttttgag cgaagaggac tttttgatgc ctttacagcc
     2701 gaaaaaatag aagtttttcc agcagaaaat acttttacaa atgacaaaga cttacaaata
     2761 gtggtttgta taccagattt ttgctacgac atagaagaca catacaaaac tgcgataaac
     2821 atagcaaata aaggctacaa agttgtttta tttgattttt ttagccgaga tacagaaggg
     2881 ttttctgtat ttagacaagg gaaaaacata aaaaactttc atgaaaaact ctacttagca
     2941 tttaagcctg aaatatatga gcaagaaaaa cagaacttga tcgagcaaaa acgaaaagaa
     3001 atgctctcag cgatgagcct catatacagc gaaaaaccag aaccnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnntattca aaaaatagcc tatgctgttg atgcctgtca
     3181 agcaagtata gaaaaagcaa tcgaaatagg tgctgatata ctgtttgtgc atcacggtct
     3241 tttttggggg aaggaggcgg tacttaaagg cctgcattat aatcgcattg ccagcctcat
     3301 acaaaatgac ctagcactct atgcggtaca tataccactc gatgcacacc cagaagttgg
     3361 tcataacgct agtattgtaa agcatcttgg tttaagcaat atcgagggtt ttggttcttg
     3421 gcgaagcatg gatattggtt tcaaggggtc ttttcctaaa gcaaaaaaca tcgatgaagt
     3481 ttcagcatta ttatttaaag gaagcgaaaa agcactacag acaatgccct ttggaaaaac
     3541 aaatatttcc agcgtgggcg tcatcagcgg gggcgcaggc ggagatgtat ggcaggcagt
     3601 cgaacaaaac ctagacctgt acattaccgg agagatttct cacgaccttt accactttgt
     3661 taaagaaaac aatatctcgg tcatagctgg cgggcactac cacaccgaaa cctttggact
     3721 caaaaacctc ttgcaaaaaa ttgaaaagga tttgcacata acaggcgttt ttttagatat
     3781 tcctacaaat atgtaaaaga atagtaactc tatcactgca ctaaaacgaa gttttatata
     3841 gtttaatagc ttagaatatt taaatataga tattttttta tagctttcgc aaaataacgt
     3901 tgcgatacaa cgaaagagaa ggtgcaaaca tggaaaaact tttaattatt gggggcgttg
     3961 ccgcgggtgc aactgctgcc gcaagagcaa ggcgcttaaa tagcgatatt gagataagca
     4021 ttttagaagc aggaagcgat gtttcttttg ccaactgcgg acttccctat tttttggggg
     4081 gagaagtaga aaatagatcc gaccttattc ttgcaagccc tgaaacattc aaagaagaat
     4141 acaatgtgac tgtccatcta aacaccgaag cacgcacaat cgatagaaaa aacaaaacag
     4201 ttaccgcgat tcaaaacggg aaaaaacgtg tttttgacta cgacaaactc attctcgcac
     4261 aaggtggaaa acctattgtc ccgccacttc caggagtcga tcaaaaaaat gtatttacat
     4321 tgtggactct ttctgacatg gataaaataa acacattcat taccaataaa aaacccaaaa
     4381 ctgctgttgt tgttggagca ggttttattg ggcttgagat ggtagaagcc cttgtgcacc
     4441 gaggcttaga tgtgcacctt gtagaactcg caccgcaagt aatgattcaa cttgaagcag
     4501 aaattgctgg ttttgttaca aaagagcttt tagattatgg cgttcgcctt cacctaaaca
     4561 cctcactgca agctataaaa aaagactctg tttcactgag cgacggttca aaaataaaag
     4621 ctgatttagt tctcctttct gttggagtcc gaccaagctt gcagctggca aaagatgccg
     4681 gcttagcaat aggcgaaaca ggtgggcttt tagtcgatga atatctacaa acaaacgacg
     4741 aagcaatttt cgcagcgggc gatatgaatg aaatcacaaa cactatatcg caaaaaaaac
     4801 aaagagtccc tctcgcaggc cccgcaaatc gacaaggaag aatagccgca gaaaatgccc
     4861 tcggcgggaa aaaacgctat aagggctctg ctgtttcatc catagtaaaa attttcaaag
     4921 cccatgcagg ttcaactggg cttagcttaa aacaggcaaa agaagcaggc tttaacgcag
     4981 atgcaatcgt tgtacataaa tcaagccata caagctatta tccgggcgct tttagagtaa
     5041 gccttatgct catcttcgat aaaaccgatg gaagaattct cggcgcccag gcggcaggaa
     5101 gagttggtgt cgacaaaaga ctcgatgtta taacgaccgc aattgcagga aaattaaagc
     5161 tcgaagagct gggagaactt gaccttgcct atgctccccc tttcaatagc cccaacgggc
     5221 ctgaacaaat ggcagccttt gttgcagaaa accaccgcat cggttttagc ccctctatac
     5281 ttgcacaaaa tcttgaagac tgggttttgg caaaaagtcc tattatcttt gatattcgcg
     5341 accccatcag ttattcacgc gcccatctct cacaaacaaa taacctaagc caaggacaaa
     5401 ttcaagaaag tctcgacagc cttccaaaag acagcgcctt gcttgttatt tctgaagatg
     5461 gacaaaaagg gcatatctta actcgcatgc ttttaacgaa gggatacagc aatgtactga
     5521 acctttctgg cggatatatt tcacttgaaa gacaagagcg cgcaaagcct tttgaaaaac
     5581 tacgcgttgg tttgcacgca attgaaaaaa aatcaataca aaaaagtagc gaaagccatg
     5641 agaaaaaagc atcagaagta aatacagctg tggaaaaaac agaaggtccg ctcattatag
     5701 atgtgcgaac tcccatggaa tttaaaatgg gtgctgttcc tggtgctatt cacgctgacc
     5761 ttgatagctt agaagaaaag ataccagtaa ttacaaagaa tgattttaat agagaaatta
     5821 ttctatactg cgcttctggt gctcgttcat cgtacggagt acgcatcctc aaaggtttag
     5881 gctacacaaa tgtaacaaac ggcggcggct tacacacaat gatgtcgcgt tttgcataag
     5941 acagccttgt aaatattgaa cgaaatcctc atcttgcaaa aaaaagcgtt gtattgtagt
     6001 attatgacat tatggaagaa ttcgttcaga tagactttaa aaaaaagaag acctctttta
     6061 caaaaagtct cccctttagc cttgcaatac tgctttttct cctcgatcaa gggacaaaat
     6121 tgctcataga gaaaaacatc cctctcaatt ctgtaggatt ttctcttttt ggcgattttt
     6181 ttcgtataat tcacgtaacc aacaagggaa ttgcatttag ccttggaagc tcttttcaag
     6241 attccgcacg gcaaatcctt tttgccttcc tccctttagt tgttcttggc tttgttgttt
     6301 ttacctattt taaaaacaac gatttctccc cctttcaacg ctggacgatt gctggcatca
     6361 tcggaggcgg ccttggaaat ctttttgatc gattttttag accaaacggc gtcgttgact
     6421 ttatcgatgt aaaattttat gggatttttg gctttgaaag gtggccaacc ttcaatatcg
     6481 cagatatgtc cgtgcttatt tgtggtattt tacttttaat ttcatttttt cttacgattc
     6541 taaaagaaaa gaaaaaacag gaaaggaacc caaatgacaa ataaacaaag aacacttatt
     6601 tttattgcag tgggaacaat tatatctatc atcctcactc tcattatttt aatactactc
     6661 attcttgtcg gctccctcct tttaaaagaa cgaatagcaa acttcctccc cctcgtttta
     6721 atgatagcta tatttctttc gctctttgtg tatcaaaaac tcactgtttt tgttgttcaa
     6781 aaatggaatt tagaggacaa actcgaccca ctttttaagt ctaaaaaaag acgtaaacca
     6841 agagactaag attttctatc aataaaataa aatctcaata gccgtcagac atagcacggt
     6901 ttaggtctcg ctgatacaat tcttgatata ctaaacttct cgttcgcaag ttttctttta
     6961 tttcttgaga aaatggcata tgacgccaaa aaacgccacg aacttctttt tcaagccttt
     7021 gcacaccttc gctttccttt gtcatccata aaatatagtc gtttataaaa tactctttaa
     7081 catcaccttt caatttttgc gtataaaacc attgataata ctgccctgtt aagccctctt
     7141 ccatccagta atacgaggct ttttcctttg ccacttgcca gcgcaaatca gctacggctg
     7201 tcaataatgc tatttcaaga tttcttggat acattggcac aacaatgcga cctcgacttg
     7261 taacacgatt atagcggtcg aagggttccc aacacattcc agaatccccg taagttggaa
     7321 ccaaaagaac atagggcact attctatttt ggttgttttt atgcacccta caaaaggctc
     7381 ctgggtcaat ggattcaatt tttgccatta aatctatcac attttctcga tatcctgttt
     7441 gtcgtggcat acaatggaaa aactctcttg ttaacagagg aaaatgattt ccttgacgtc
     7501 caacagtcat tttagccatt tgtcgaacag tatctatctc ttgtaaaata tcgcttgtat
     7561 ttacttcaca atcttcaggc atagcttgta gctttacttc taaagattct gcaactccct
     7621 tcgcatctgt gtattcttcc aaatatgatt gaatttctcg atctattttc aacaaacttt
     7681 ttaagtcttc tccaatactc ggaaccaatt ttttttgggc ttctgttaaa aagcctttat
     7741 gaatatcatt tccatatgaa ggatgctcac aaaacttatc tacttttgat tttaattcta
     7801 tctccaacat agaacgctct ctctcttttg ctgtaagcat tcgttccgac gcttgcaact
     7861 tcccttcatt ctttgcctgt aactgcctta atcgttgtgc ttctgcattt ccgccactgc
     7921 tgcctttctt cgtaggccgt gcttcgtccg ttgcagaagg agccattttt ccattcccaa
     7981 tatcacgaaa ccattcatca acataaaata caggttctcc cgtattattt tttggaaaaa
     8041 cacttgcaaa ggtatctttt tgctcatttg tcaaaagcga tggcagaaca agtccaaatc
     8101 gcaaaagcat ttttttctct tcttctatgg tcaaagaaac tgtctttggg gcaatagagc
     8161 gaacaagttc ccagtacaag gtatttaact gttgcctaaa aacagatcga tcctttggat
     8221 ctttcgatgt aagatattta gataaaacag tatgaaaacg ttgcgctgct tctgtttcgc
     8281 ccgcaagagc tgttttataa taatttggat tgccaaagac aggatttaaa tccgaagaaa
     8341 aaaattctgt aataggctta aaccccgtta aagaaaggtc gacctctaaa tgagaatctt
     8401 ttttttcatc gatttttgga ctaccccaac tattttcttt ttgaaaaccg agactttcat
     8461 cctcatctat cttagacaga ccaaaagaag gtttcttcgc ggtttctgcc aaaagtgctt
     8521 ctatttcagg tgataattga tcgttcatag aaagattata acattttttc tcttaggttt
     8581 caaacatttt ttatgtaatc gatatatttc catccacaag aggtgagtct gttacttact
     8641 ttttactgct ttttaagtat acttttaatc atgaatagat tcgatgttat aattataggc
     8701 aaaggacctg cgggaataag cgcaggctta tatctcaaaa gatctcgtta tgaagtcctt
     8761 atcattggaa aagattttgg ggcactcgat aaagccgaaa aaatagaaaa ctattatggt
     8821 cttgaaaacc cgataagcgg caaagaattg tctgaaacag gcataaaaca agcagaacaa
     8881 ctcggtatag atatttttac ctgcgaagta acctccgttg aagccacttt tgaagagccc
     8941 tatcgcttta agatttctgc aacacagggc gaaaaaaaac aagaatttct ttcaaaatca
     9001 ctgcttattg ctacaggaaa gcagagaaaa acacttcgca ttccaggttt tgatgatttt
     9061 aatggcaagg gaataagctt ttgcgccact tgcgacggct ttttttacaa aaacaaaaca
     9121 gtcggcgtcc tcggcgcagg ctcctatgcg atgagcgaac tctcctatct atataatcta
     9181 agccaagata ttctactctt tactaacggc gatccacaaa tagaaaaaac cctcttagac
     9241 tctccgcttc cagaaggcgt tcgcttaatt actgataaac taagccaaat atacggaaca
     9301 gaatatcagg ataatgtcag tgttctcgct ggagttgaaa caaaggacag cgaaggaaat
     9361 atacaacgcc acgatctcca aggtcttttt gtcgccctcg gaacagctgg tgcatccgaa
     9421 tttgcaacaa aactcgggct tgcaacaggc gagggaacca atgccggcac catactcacc
     9481 gatgacggaa tgcgaacaaa tgtccctgga gtttacgctg caggggatgc aacaggcggc
     9541 tttttgcaaa tagtaaaagc tgcaagtgac ggcgctatcg ctgcaaaatc aattggaagc
     9601 gacttaaaag agctttcacg ctaagttttc ttaacgatta ttgccctttt ttcttttttt
     9661 tattaccttt aaagcataga cctaacactg aggttcttac attatatttt taaaagagga
     9721 aaaaatggca aagtatcaat tccaaacaga agtttcacag cttctcaaac ttattatcca
     9781 ctctttgtat tcaaacaaag acattttttt acgagaaata atctccaatg catcagacgc
     9841 ccttgataaa ttaaaatatc tcaacctatc tgatgaaaac tacaaatcca tcgtttttga
     9901 cccacgaatt gatataaaat tcgatgagaa agaaaaaact attaccgtac aagatagcgg
     9961 tatcggcatg gacgaagaag acttaaatgc aaaccttggt acaattgcac gttctggcac
    10021 aaaggctttt ttagaaaacc ttgaaaaaga agcaaaaaaa gattctgctc ttatcggaca
    10081 gtttggcgtt ggtttttatt cagcctatat gacttcaaaa aaaattgatg ttatcacaaa
    10141 aaaagctgca actgaaaaaa tcctcctgtg gtcaagcgat ggaacggaat cttacgagat
    10201 agaagaagtc gcaaaagatt ctgaaaaagc gaaaaacctc ggctttgaca aagacggaag
    10261 caatggaaca gccattatta tgtacttaac agatgaagac gcttcctatg cctcacgctg
    10321 gaaaatagaa gaacttataa aaaaatacaa cgaccacatt gccttcccta tcttcttaca
    10381 ttttgaacaa aaacaatacg acgacaaagg caaagaaact tcttctgaag caaaagtcga
    10441 acaaatcaat agcgcaagcg cgctttggaa acgttcaaaa tctgatctca aaacagaaga
    10501 ctataacgaa ttctacaaag caattgccca cgatagcaat gacccgcttc tcacgatcca
    10561 cacccatgct gaaggaactc aagaatacac aacactcttt tacgttcctg aaaccgctcc
    10621 ctttgacatg tatcaagcgg actataaaag cggcttaaag ctctatgtga aacgtgtttt
    10681 tattaccgat gatgaccgag aactcctccc atcatacctt cgatttgttc gaggaataat
    10741 tgactcagaa gaccttccat taaacgtaag ccgagaaatt ttacagcaaa atagaatttt
    10801 gtcatcgata caaaccgcaa gtgtaaaaaa gcttttaagc gaatttaaaa aacacgcaga
    10861 agcatcagac aaggctctag aaaaaaaagc cgctctttct gaaggcgaat ccttgagcga
    10921 cgaagagcaa aaagcaattg acagatggaa tacctttata agccaataca accgaccttt
    10981 aaaagaaggt ttgtattcag attatgcaaa cagagaagaa ttaagcgaaa ttattagatt
    11041 taagagtacc gcagaagaag gaaccggaga aaatgcgtgg acaagttttg cagcctatgt
    11101 acaacgaatg aaagtcgatc aaaaagcaat ttactacatc accggtggag atgaaaaaaa
    11161 tttacgttca tcaccgcttc tcgaagcata caaggcaaaa ggcatcgaag ttctcatcat
    11221 gtcagatgat atcgatgaca ttgtaattcc atctttgatg aagtacaaag acttcgacct
    11281 aaaggctgcg aatcgcgcag gaagtgatga ggagctcgga gtcgataaaa aagaagctga
    11341 gaaaaaagaa aaagaattta aacccattgt tgaaaaaata aaaacagctc ttggagacaa
    11401 ggtaaaagac gtgcatcttt caaaacgctt gtcagactcc ccttcttgta ttgttgttga
    11461 cgaaaatgat ccatcattgc aaatggaacg aatgatgaag gccatgggac aagcaggctt
    11521 tagtgaagta aaacctatcc tcgaagtaaa cggagaccat gcccttgtcg cacaactgaa
    11581 agatacagaa gatgcggcct ttatagaaaa tctttcaaat gtgcttttag atcaagccct
    11641 tcttttaagc ggtgcggaac tcaaagaccc agcagatttt gtaaaacgct tgaacgcact
    11701 tttagcaaaa taaataaaag cttgcaatac taaaaaaagc tatcgattta aaaccgatag
    11761 cttttttatt ttaaacagct cttttacaat aattttggta tttatctaaa tacttttatc
    11821 tctttacttt cgaacccaaa gtcgctcatc taagggaagg gtcttttttt catcagcttt
    11881 tgcttctaca gaaccaaaag gcatttgcga taaaagtttc caggactctg gaatctgcca
    11941 ggtcttgcgc acagcttcat ccaccagtgg attgtaatgc tgaagcgaag ctcctatacc
    12001 ttcacttgca agactcatcc aaatagcata ctgcaacata ccgctcgact gcaaagacca
    12061 caagggaaaa tgttctgcat acagtgaaaa atcttttgcc agtttgttaa caacattttg
    12121 atcttcaaaa aaaagcaggg ttccaaatcc agaatcaaag ccgtccagtt tttcccttgt
    12181 tttttcgaag gcatctgccg gtacaatttt ttctaaaaca tctttagtaa gagaccaaaa
    12241 ctttgagctt tcttccccaa aaagaaggag aacccgagca gattgtgaat taaatgctga
    12301 gggtacatgt gtcagcgcaa aagaaactat ttcttgaact cgatcctctg aaataatttt
    12361 attttttcca agattataaa tactgcgcct ttcttgcatt gcatcataga tttccatatc
    12421 ttcctcctac tttcaaataa ataatacata tctaagctaa tatagcatat tcctagtgat
    12481 ttgctatgta atcaagtcgc aaagattttt ccctttataa cgtctctttc tcaagggggg
    12541 gagcggaaaa ccgtttttgc aaaaaaacgt ttgaagcgag ggggaggtca ccccctcatc
    12601 caaattttct ttcccagagt ttttttaatt gatagtatcg tgggcttaaa tctgcaagtt
    12661 tttttgcgcc ttgtttttct gcggctttta caataatctg tgcgcaggtg tctgagtcgg
    12721 caagggcgtt gtgggcttcg tactgaattc caaagttttg tcctaaaaag gtgagtttat
    12781 ggcaggctaa ttctggccag gttttgcgtg ctataaatac ggagtcgtaa tattgaattg
    12841 aggggaggtc gagcttgtag tgggcgatga gggcgcagag aacacccatg tcaaatcctg
    12901 cgttatgagc gatgacagga aagtctccga taaattcttt taccgctgcc catatctggg
    12961 aaaaaagggg tgcatcttct acgtctttta ctcgaagccc gtgaatttcg gtaaaatcgg
    13021 ggcgaatata gagttttggt ggacagatga gggaataata ttcgtctgtt tttttgccat
    13081 ttgtgtattt taccaagcca atagatacgg ctgagtttgg cgcataatct ccagtttcaa
    13141 agtcgagggc aacaaaatgc atcttctttc cttatattcg ttttatttta atgaggatta
    13201 tatcttcagt cttattgtat atcttttttt ctaaagactt ttacggtgag gtcaacgact
    13261 agaattaaaa tgcccataaa gacaaaaaga acagccattc ctatgcccat aagtaaaaaa
    13321 cttttttgaa gtgcaagaaa gtccatatta acctccaatt aaaataggta tgagtgcgag
    13381 aacgagtccc cctgcaacaa cggagccaag ttgtcctgaa acatttgcac taacggcatg
    13441 cattaaaata atatttgttg ggtcatcttt gagcgccatt ttttgaataa cgcggctaga
    13501 catagggaag gcagaaatgc cacaggcgcc aatcatgggg tttattttct ttttcaaaaa
    13561 gagatttatg aattttgcaa aaaggacacc gccggctgta ttaaaaacaa aggcaaataa
    13621 accgagtgcc atgatgagaa gggtttctgg tcgcaaaaag ctgtccgccc tcatagaagc
    13681 gccaacggtt atgccgagta aaatcgtaac gatgtttgac aattcgtttt gggcggtaag
    13741 agaaagtttt tctaaaacac cgctttctcg gatgaggttt ccaaacatta aaaaaccaac
    13801 gaggggcgct gcaattggaa taaggattga gcagattaag gttaccgcta tgggaaatat
    13861 aacaaaaaca actcggggga tactgtgatc gactgcggtc atgcgaataa gtcgctcgtt
    13921 ttttgttgtt aatgccttta tcaccggcgg ctgaattata ggtaccaagg acatatacga
    13981 atatgcgaca accataattg gtccaagaag gtcttttgca agctttgttg taataaatat
    14041 tgttgtcggc ccgtctgctg ccccgattac acctatgcag gctgcttctt caagcggaaa
    14101 gccgagggca actgcaagag aaatcgttgc aaagattccc aagtgggcag ctgcaccaaa
    14161 aagcatgagc cagggtcgtt gaaagagcgg ggcaaaatct atcatagcgc caacgcctat
    14221 aaaaataagc aagggaaaga gttctgtcaa aacacctgct tcaaaaagga tttttaaagc
    14281 gccaggtgct ccgtcatggc tccacaccac ttccaaaggt aagtttacca agatggcacc
    14341 aaaaccaatg ggtagcaaaa gcatgggctc atagtccttt gcaacagcaa gccaaataag
    14401 cacaatgccg atgaggtaca tgagaacttg ctcataacct atagcaagga tgccttcaag
    14461 aaaaaatgca aatatatttt ctaacaagtt aatctcctag ataacagtta actcattcta
    14521 aaccgaaagc gcttttttta caatggaaaa caaatctttt ttttcaaaat tttatttttt
    14581 ctagcttttt gaaaccgcac aatgtcttag catatcgacc ctatagctct tgcgaacctt
    14641 actttttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctt tttggttaag
    14761 ccgaatcgta tagacaccgt ggggatgcag catctttttt atttcatttg tcgcttgttt
    14821 aaacaaaaca gtccagcgaa caacctttat accaccagcg gtggagccgg atgagcctcc
    14881 gataaacatc aaaaagaaaa gaagactttg tgcagctcct ggccaaagcg taaagtctct
    14941 tgttgcaaaa cctgtagttg tgagtataga agcaacttga aaaaaagcat ggcgcatggc
    15001 ttcttctatt tttccatata aaggatagat ggataaaaac acaaaaaacg aagcaataaa
    15061 aataatagaa aaataaacct tgcattcggt gttttttaat agctcttgcc tttttttatt
    15121 ccatagtaaa aaatacaaac taaaattcac gccggctaaa atcataaaaa cagtacatat
    15181 ccactgcgca cttgcagaat aacttgcaag gccgctattt ttcatagaaa aaccgcctgt
    15241 tccaagcgtt gcaaagctat gaaagactgc ttcggtaaag ctcatttttg caaataaaag
    15301 aagaatcact tgtaaaaacg tcagtataac ataaaaaatc cacagttttt ttgccgtttc
    15361 acgaatcttt ggtgttattt tacccttatc tggtccggtt gtttcggctt ttattagctg
    15421 aaaaccacct atgcccaaaa gcggcatgag cgcaactgtt atagcaacaa ttcccatgcc
    15481 tccaagccag tgcatttctg cccgccatat attgagcgaa aaaggaagcg cttcaacaga
    15541 gcttaaaata ctcgctcccg ttgttgtaaa gccagaaacg ctttcaaaaa aggcatcaaa
    15601 aaaagaaggg atatagccag aaatcataaa gggaatggaa cccaagaggc aggaaaaaag
    15661 ccaggatagg gcaacaaccg caaagccaag gcgcatgggt agacgctgtt tttttttagc
    15721 cttgaagaaa aaagccaaaa gaccaagtaa aaaacaaaat gctatgggaa taataaatga
    15781 aagatagacg tcaacttcgg aaaaataaat ggcaaaggct aagggtatga gcataaagca
    15841 ggcgatgata cagagcaaaa aaacaataat tcttgctaaa aaatacatca ttgttttaca
    15901 ccaaaacctt gcataaattc tttatttttt tctctatcgt acaaaacaat gagagaatct
    15961 tgtgcaaaaa gcttcgtatc tccagtggga atatggtaca aaagctcttt ttcttttttt
    16021 ataagcaata ctaaaaaagc agcctctgca gaaaggtctt ttaagttttt tccataaaaa
    16081 ggagagtctt ttagcacgct caattctgca atttccatct caccttcaaa aagcgtatgg
    16141 attctagaaa ttgattttcc cctcaggtga ctcataatag catctataac cacatctttt
    16201 atcggaatag caacatcaat acctatgcca tttgcaattt ttgcgtattg cgcatttgtt
    16261 acaaggcaca cggtttttat atctccaatc gatttgagat atgcggctgt tactatattt
    16321 aattcatggt tatgagttga ggcaattaaa acatcatagt tttgcaagcc ttcttcttca
    16381 ataaaaccct catcgctaat atctgccttg tagactgtcg cttcgggaaa aagctctgcc
    16441 gcattttttg caagttgtcc gtctttttca ataatcgcaa attccttgcg caatttttga
    16501 ggaacagaaa aaacatgtga aaaaaaccgt tttttctttg cctgcatgag gcgtcctgca
    16561 accctatttc caatgcggcc ggcacccaaa atagcaattt ttcgtattgt ttttttttct
    16621 tctccagcaa gttgcaaaag gctttctacg tcttcatcct tcgttaaaaa accaatgcga
    16681 tcgcccgctt gaaagattgc ctctccattg ggaatataag ctgttttttc attttcaata
    16741 tataccgcaa gaaattgttt ttttgtaatg gattttaagt tttgtatagc aagcccgtca
    16801 agggcagaat cttttttaac taaaaccgaa gctatttgat aatcgcttcc ttcaaagtca
    16861 atactctccg aaaccgtatc ccattttatc gcatttacaa ttgcctcagc tgcttctaga
    16921 tctggctgaa tcataaagtc aataccgtag ggtggacgac gatttttttc tgcatttttt
    16981 ttgctctgta ttcctatgtc attataataa tcactgtttc gtacacgtgc tattttaaca
    17041 acctgtggat aaaccgagtc gacaagagag cagctaatca tatttagctc atcactttcg
    17101 gtaagcgcaa caaggaaatc agctgtttca atgccaactt cttctagaac gttaagatta
    17161 tttccatttg cttgtaagag catacaatcg agccgattcg atgcgtgccg gatggtttct
    17221 tcatccttat ctataagcac aacatcttgc ccttctgata tgagccgttt tgccaattca
    17281 gttcccgtaa aaccggcacc gattataata accttcattt tttgtctatt ctatttcctg
    17341 ttcgagtttt gatttttttc taccactcac atcatataac gaaacgacag caatggaaag
    17401 taccacagaa gcacctgctt caagtaatgc ttgtggaagt aacataatta aaaacgcaat
    17461 ataccccgca ccttctgtaa tcgctacaag gtcggtataa tgtgttgcat acaaggcacc
    17521 gattacaaaa acagtattcg ttaatgaacc caaaaaggca gcaaccccaa caaagccttg
    17581 tttcaaaaaa gggctgttgt ttttgttcat actctgcaaa gccttaaata caaaaaatgt
    17641 tataagagca ataagcattc gaggtagaac agaaacaata gggttcacga aaaagggatc
    17701 gagcacaccc gttgggccaa cggcagcttg aattaaactt gaaagcccaa aaaccaagcc
    17761 aactcccaaa cctgcccata agccgccgag caaggtagcg ataattactg gtacatgcaa
    17821 gatagtgatt gcggcaccag aaaaccatgg aataaagcct aaacgagtta tcgttagaac
    17881 aattgtcaaa gccccaaggg ctgcagtgac cgcgagtgcg cggatcattt cttggttatt
    17941 tgttttcata aaattagttt aatgtaaaac tatttttatt gtcaatagct agtatgctat
    18001 gaattttttt taccttgctt aaaatcttca ccagttagat cttgaaacac agccaaatca
    18061 aaaaaatgca taacagaaag cacatggtcc aagacatcac tttgtaagcc ttcatacgga
    18121 acccagtttt tatttgccga gaaaaaatac agctcaacag gaattccttt tgaagtaggg
    18181 tcaagataac gcaccataaa ggtaagcgtt ttactcacaa ggggatgttc ttttaagtat
    18241 tcaacgatat aagctcgaaa ggttcctaaa ttcgtcaatt ttcgtggcgt aatttcatcc
    18301 tcacttgaaa ggttaagttt ggcgttatat tctttaattt cacttcgttt ctcttgcaaa
    18361 tactctttta atcgaggtaa tttttcaaga cgatcaagct cttcttcaga caaaaagcga
    18421 accgttttca tatctatata aattgatttt tttactcgcc gcccagcact ctcactcatc
    18481 ccccgccagt ttttaaaact atctgaaaca agtgcataga caggaacact cgttatactc
    18541 atatcccagt tttgaactcg tattgtttga agattgatat ctaaaaccgc tccgtctgca
    18601 ccgtattttg gaatttcaac ccaatctcca atgcgcacta aatcattgcc ggtaagttgc
    18661 aagctcgaaa caaagcccat tattgggtct ttaaatacga gcatgagaac agccgaaagc
    18721 gcaccaagcc cactcaacaa tcccaaggga gaaacatcta tcaaactggt tataatcaat
    18781 ataaccgcaa ctatatagag aaatattttt accatttgca aataaccttt gataggtttt
    18841 cgttttgcaa gttctggacg aaattgcttg taaatttcat ttatggcatc taaaaaaagc
    18901 gcaattgttt ttgccgcggt aaaataaaaa aaagcaacaa tgagccgttt tataaagctt
    18961 gctgctaaaa attcgtctaa aataaacaca ggatataaag tcaatataaa tataaaactt
    19021 cctacaagta taacttgctt aaaaataccg aggtcccaaa taatatcatc ccaagtaatt
    19081 tttgattttt ttattgcctt actaacaaca agcttattaa aaagagaaag aatttgctta
    19141 aaaataaaag ttaataaaaa taaaacaaaa taactgatta aaacaataat aatagattgc
    19201 cattcttctg aaacctgaaa tatagctatt ttttcttgaa taaaattata aaacatagtc
    19261 tcattgtagc atttttataa aaaaatgaaa cctttttttt atttccatgt tatacttctt
    19321 ctatgtataa ttctaaaatt ggcattgcga cctctttttt cgcagatcaa aactttgact
    19381 cttcactgac actcgccctt gcacacgaag aaaatatctc ttgggtacaa ctttttttaa
    19441 gtccttatac tgaaaatccc catgaaattt ttaccctaca aggacttgcc acgcaacacg
    19501 gaacaaagat tatctgccac agcccgcttt tattaaatct cgatgcaaaa gatggcatac
    19561 atgcagcgaa tttatccttg tttttcccgc aaggacaaga aaagttctct gtctttcact
    19621 ttgacgaaaa tgaaacaata gaaaatgccc tctccgtcat tgagttttat tccacaaacg
    19681 gttttactat atgcctcgaa aatttttacc aagataaaag caaggacgcc tttatacaaa
    19741 attttcacga ttatcttgct cttatagaag ccgctcaaga aaaaaaatac aaaatcatac
    19801 ctgttttgga ttttccaagg ctttttattg aacccattac agaggcggtc gatgccctcg
    19861 cttataccga gcttttatta gacaaactcg caaaaacaac aaaaaatctc atcttacatc
    19921 tcatcgattt ttcaaaaagt agccaaaaac gggaagaatg gcttcctttt atggctggca
    19981 ttatgcccta cgaaaaaatt tttgaccttt taaaaaaata taatttcact attatttcag
    20041 ctattttgga atatgaaaac aaagaaattg gcaggcaaag tttccaagcc ctatccgaaa
    20101 gccttgataa actagtatca aacaaaagct aaacgagaaa aatcaaaggc tttaaatgaa
    20161 gctttaaata gtttagaatt ggtcaatgat agtatttttt ttaattggca ttctagctat
    20221 atctcttttt tccttttcgc tcagtgcagg ctttattttg ttaaatgcgc aaataaaacc
    20281 ctatgacaca agcgaaaagc tcaaagaaga agaaagcgca atgcccggca tgccgacaga
    20341 gcttaccgcc cacgttcgcg atcccaagat tatgacaagc ttacttgctg caaaaaatcg
    20401 atggtatgag aatcccatag aaaagctcga acaaaaaagc tttgatttga aaccaatcta
    20461 tgctgacttt tgggaatata gcaaaaaccc aaaagctact gctattttaa ttcacgggca
    20521 catggacagc gcccgcggaa tggcctatct tgccgaagaa taccataaaa gaggcttttc
    20581 tgtcctttca atcgatatgc gagggcacgg aagaagtaag ttaaagtcaa gcttcggcta
    20641 caaagatgca aaagacatcc ttctttggat agcattttta caaaaaaaat ataatattga
    20701 tcacaaaata atcgtacacg gagtttctat gggttcctca agcgttttgc gccttcttag
    20761 cttgaaaaaa gctcctgcaa acatagcact cgccgtagta gattctagtt ttgcatcttt
    20821 tcaaacacag ttaaaatccc aaatcgacaa tcttttgcca aacaattttt tccaaaactt
    20881 tgtaaaaaaa ctcttgctct ttggcttgag ttgtagtaat tttttagtac aaggcttttt
    20941 ctttttccaa catgcaccca tacaatatct aaaaaaacga aataaaagca ggtccaaatc
    21001 tataccaatt ttgtttttcc acggcttaaa agattccatg gtgccagtga aaaattgcga
    21061 aatactattc gccctcacaa atgaacctaa aaaacagatt ttgatagacg atgccttaca
    21121 cataggtgcg tatttttatg ctcctgaaaa atatatgcag gaaatagaaa atcttttcca
    21181 ctaattgcat tataaaatag aataaataaa tagaaggaag gatttaaaaa ataaaaaggc
    21241 cgctcaagga ggaggaaagc gaccttttct aacgttttgc tgtatgttga tatactataa
    21301 aaaattattt tttttgtcaa tacgatttgt tgacatttgt tgattttttt cgcaaatatt
    21361 gctatatttt acaaaacagg ggatattatg acattattcg gcgttattgg tgcaatggaa
    21421 gtagaaattg cacttttatt acaaaaaatg gaagaaactg ctgcggtaaa aacaaaacag
    21481 atagcttctt taacctttta tgaaggcgaa ataggtaagg ctgcacttgt tttggtaaaa
    21541 tcggggattg gaacagtaaa cgcagctctt tgcgcgcaaa tccttatctc ggaatttaag
    21601 gtaagccatc taatcaatac aggcatagca ggggctcttt caaaaaactt gggcgtttta
    21661 gacgtcgttt tgtcaacaga tgcagtgtac cacgacttca gggtaactgg ctttggctac
    21721 aaaccttgca ttatacctgg gatggaaaaa tccgtgtttt cagctgattc aaagctcatt
    21781 gaaaaagcaa aaaatgccta cacttctgct cattttgtcc acaacttata cacagggcga
    21841 attgcaacag gaaatctttt tgtcgatcaa acaaacgaaa aagcaagaat cattagcgac
    21901 gtaagcgact tggacggaat ctctccaatc gctgtagaga tggagggagc tgctattgcc
    21961 cacgttgctt atgtaaacca aattcctttt ttaattatcc gaagcatttc tgacaccgct
    22021 gacgaaagta ttgaaaaagg agaatacaag gaagaagaag ctgctaaaat ttcttcattt
    22081 attgtacaat ctattttgga ggattaatta tgaaaaaaaa atacaatgta gaaagcttta
    22141 atctcgatca caatgcggtt actgcaccct atgttcgcct tgccgcaagc tatactggcc
    22201 caaatggaga cattgtaaca aagtttgata ttcgttttac gcagccaaat aaagcctttc
    22261 ttccaactgg agcgatgcac acaatcgaac atcttgttgc agaatatatt cgagatgaag
    22321 tttctggagt aatcgatttt tcacccatgg gctgccgaac aggcttttat tttactcttt
    22381 ttggaaataa aaccgaagaa gaggtaagcg cgcattttaa agcggtcttt caaaaactcc
    22441 tcgactggcc cgatacagaa aaacttcctg gctacgaccc aaaagaatgc ggcaattacc
    22501 tcgaccacaa tcttgcagaa gcaaaggcct atacaaaaaa atggcttgaa ggcatagaaa
    22561 aatacggttt ttcatgctat aaataaaaaa aaaggactat actattttat gattttaatt
    22621 ttttcgcttt taattatcgt ttctatgatt tctacaaaac taagctcaaa aatgggaatg
    22681 ccccttttag ttggttttat tctcgttggt ataatagcag gaagtgatgt tttaaaccta
    22741 ctttactttg acaatgctct tatggcaaaa caaatcaccg atattttgct tatttttatc
    22801 atctttgaag gcggttttcg ggttacaaga aaaaactttc ataaaactgc tgctccctcc
    22861 ctcacccttg caactctcgg cgtaatgctt acagccctgg cacttggtct ttttatttac
    22921 tttgtattaa aacttgattt tctaacctct tttttaattg cttctattat ttcatctacc
    22981 gatgccgctg ctgtttttat ggttacaaaa gaaaatccca taaatcccaa acttgccaca
    23041 acgattaatg tcgaatctgc tgcaaatgac cccatggcaa ttctccttac agtaaccttt
    23101 atccaacttt taacagaagg aataaaatct cccctaacca tcatcatttc acttacctgg
    23161 cagtttttag gcggagttgc catcggctac ctcgtacaca tagtcacaat ctttgttttc
    23221 aataaattta actcagacaa ccgtggcaat tacaatgtcc tcatcatcgg gcaaattctt
    23281 ttttgctacg gcttagcggt gctttgcaaa gcaaacggaa tcattgcggt cttttttatg
    23341 ggttattggc ttggaaatgc aaactatgca gcaaaacagg gcgtctctaa ctttcttgaa
    23401 agcatcgcct ctatgtgcaa tgtgggactt ttcttaatcc tcgggcttct ctcttttcct
    23461 agccgtttta ttcatatttg gaaagaagga gttatcgttg ttctcgtcct catttttatt
    23521 gctcgcccca tcgctgtttt tctttgtact atacccttta aatacaattt taaagaaaag
    23581 ctctttataa tttggggtgg cataaaggga gcggttccca tcgttttagc aacctatcca
    23641 gcagcttacg gcctcgatga aaatggctat atctttgaca ttatcttttt tgctgttttt
    23701 atctcctgtc tcctgcaagg ttcaacccta ggcttgcttt caaaagcatt caattttacc
    23761 ataccaaaaa gacagacaac gccctatgtt gtagaacttc actcaaccca agaaaccgat
    23821 gtcgaaatgt ttgaaattca catagatgat gatgcacaga gcataaacaa aaagatatca
    23881 aaacttgacc ttggacaaga tgtacttatt tcttctatca ttcgtgatgg aaaaattgta
    23941 ttaccaaagg gcaatgtttg cataaaagca caggatattc tttttatctt aacctcgaca
    24001 gaaaaaattg atgaaataaa tgcaattctc aacaattaga accatttttc taaattggat
    24061 actatgcaaa aaataaaagc gctcaataaa aacgaaattg aaaaacacta tcaagcattt
    24121 tctcccctag aaattaagcc aaaaatccat atttttcctg agctagactc caccaactcc
    24181 tatgcaaaac aatttttaaa agaaaatccc ctcgaaagtc atggaagcat tattatcgca
    24241 gaaaaacaaa gtgctggacg aggacgattg ggacgcagtt ttacatcaaa cacagatgaa
    24301 ggactctata tttcctttat tttaaacaca gacaatcttc ccgttccact tataacgccc
    24361 tatgtaagcc ttgcgcttgt acgaagtata aaaagcattt ggaatatcga tgcaaaaata
    24421 aagtgggtta acgatgtttt tatcgggaca aagaaaatag ccggaatatt aaccgaagcg
    24481 agcttttctt cacaggaaaa aggactttcc caagttattg tcggtatagg tataaatatt
    24541 tttcaaaaag aaagctgttt ccctgtagaa ttgcaagcaa ttgcaggctc catctaccaa
    24601 gcacttttag atagggcttc tgtatctatc gattctgaaa acaataaaac cgaagtcgat
    24661 gaggacttca tggaattatt ctttgcttct aaaaatatat ttactgcaca acttatccac
    24721 aatgtgttta gaattcttac acaagatatc cacaagatat ccgagaatat cgctgaatat
    24781 aaggcctatt caaatatttt aggaaaagag gtccttgttt tacagggcga taaaagtttt
    24841 ttggcaaaag ctgtggatat aaacgaagaa gggcatttaa ttatagaaga cgcattggga
    24901 aaaaagcaag aaattttgag cggagaagta agtattcgcc ttcctactca ataatgccat
    24961 cgcgaattga gtacaaacat ccgcaataat cttgacgata gagaccaaac tccttgctca
    25021 attccaaaga acgtaaaaaa cccttgtttt ttttaaaatc tgcatataaa aatcgtggtt
    25081 tatttcccgc ttttttagct attgcgccgt cttcaagttg ctcagattcc aatagctctt
    25141 cttccaaagc cctacctatc gcatttattt tttctgcatc cttatgcgga cttatcgaaa
    25201 gcgttgtcgt tatataatca aatccatgct ctcttgcata gataaaggct ttttttatac
    25261 gaaaagcata gcaacgcctg caacgctcac ctctttcgcc ttctttttgt aaatctgtct
    25321 ctgttttcac tcccgttgcc tcgtaaaagt cagaagcttg atatgttttt tctgtaataa
    25381 cttgaatgct attattgtat ttttcaataa atgtaagcaa ttcgtctttt cgccgctcgt
    25441 attccgcctg tggataaata ttgggattat aaaaataaag ggaaatagca aaggtttttt
    25501 caagatactc taaaacatac gaagaacaag gaccacaaca agcatgtatc aaaagactcg
    25561 gctttttagt ctcagtaaaa cagggatttt tttctatttt ttgtatttct tcttttaacc
    25621 atagatgagc tggctttttt gtcatcatac aagttccaag cgaaatactt ttgattttcg
    25681 gtttttatcg attttctctt gctttgcaag aggaatactt tccagaatat ctggcttaaa
    25741 gccttgcttt tcaaaccaat ctgccgtttg tgttgtcaaa acaaaaagcg aagcaattcc
    25801 caatccccgc gccttctttt ttagaaaatc tatgagcttt atgccaatcc ccgccttatt
    25861 gtatcgctcg tcaacaacaa ctgcagctat ttctccctgc tcctcgccat aaacatggag
    25921 cgcagcacag gcacgaattg ctccgtctat ttcgtatacg acaaaatccc catgcaattc
    25981 ttttaattgc acttctgagc gctctaagag aatgcctttt tttacaaagg ggcgcatcaa
    26041 agaaagcaca tcggctacat cttcgaggtc catcgagcga attgcaccgt aattatcgtc
    26101 ataaatcata agcccggagc caagctccga aaaaagctca tttaaaataa ctccgtcaat
    26161 tctttggtcg attaaatgca ctctttcaag accctttttg catgcttcca gtgccaggtt
    26221 caaaagtcga gctgcatgaa tcggaattgc cctgttttct tttaagatag cttccaaatt
    26281 ctgcacagaa agagcattta ttgactcatt ttcgtgagct tttttttcca aaaagatttc
    26341 ttccagttca tagtcattta agaaaaaaag tttttcactt tttagctgta ttgctatttc
    26401 ttttgctaaa tcaatactcg atatattgta ggggcgtccg cttgagctcc aaccgaggca
    26461 aggaaaaata ggaataaaat tattttccaa aacagaatgt atagaaggaa gatctatttg
    26521 gtctatttca ccgcaagatt cgtagtcaaa tccatcaata atgcccttac cacgagcttt
    26581 cacccaattt cctatcaccg cagaaatttt ttctgctgcc aaagctgtca ttatggaatt
    26641 tgcaacatca aaagaagcca tttttatatg aggcattgcg ttttcaccag aaacacgaag
    26701 cccgtctttt tcttgccaca aaattcctgc actttctagc aagcaattta tccgtttttt
    26761 tgcacctgca actaaaacaa tacgaatgcc agcattgtgt atgagcgcaa tatcgcttat
    26821 gtggttgaga aaaaccgaac tttccaaaat actatccggt aaatgaataa caaaggtcgt
    26881 atttttaaat cgtttgagat agcgaataac tgagcgtata ttttgagcgt tttctagaat
    26941 ttttggtgtt tccatacaaa tagtaaacaa taaatttata aaatatgcta taggaaaaaa
    27001 aagtacaaat attaggctgc tataggactt gtcggcgttg gtgtatcatc tggattattg
    27061 tcaaccgaag attccaattc tatatatttc aaaagaaaaa gcattttttg tatagcgtgc
    27121 ggaagaatat ctctcatagc tgtcgtacgt cctgcattat caggttgtat cgataaggtt
    27181 tttctcaaat aatgcgacat caccggaggt aaggccacta ggctttcgcg caattcttcc
    27241 tcggtatatt gttttcttac aaaacctgct ttaaatgctt cttctggaat gtatttacac
    27301 ggcttccaac gaattttccc atcccttcct gtatcgcttg tataaccaaa taatctacag
    27361 attaatgctc ttccttcata caccgtacaa tgataatcat cattagggtc aaataaaaaa
    27421 catccttgtg gatattctaa aaagcgtggc catatatagg tttgatctgc tatagcttct
    27481 gccttttctc gatcattttg tattaaccac aaggctaagt aatcggcttc tatttccaat
    27541 aaatctggtt caaatgcgat gcaacattgg ccgcaacctg agggacattg agtcggagaa
    27601 tgttttttcc attcttcttc atctttttca atagcttcgt atatctgttc taattctctt
    27661 aattgtgaaa ataaaagact ttttttgaat tgcaataatt ctagcataag aaaaaaatag
    27721 caaaaaatta aaaagatagc aagatttcga gacttctatt tattcttttt ttattaaaat
    27781 aaaaagcgta tactcgaaga aaaacttgaa atatttagtt gcacaaggac aggcttttct
    27841 gcaccattgt tttttcggtt tatgttagtt tctattcgat gcagataggg gattataaaa
    27901 ccagaaaaac tacccagaat agctccacta agcacatcag taaaaaaatg atttccactt
    27961 gctatgcgca aggccgccgt cgttgtggca aaagaaaaac taccaagaat aagaggccac
    28021 ttccactgcg attcggggta atatcttgca aagacggagc ttaaaaaact tgctccagta
    28081 aaagccattg tagtatggcc agaaggaaag gattttttga aatccgtatc atcggaaaag
    28141 ggagcgcctt caaaataagt atagggtcta tagcgaggaa aaagcgtttt tcccagattt
    28201 tttaagccat agctaagaaa aatcgattca gcgtacataa caccgatggt aagccagttt
    28261 gttttttctg tacacaataa aacagcaggc aataaaagac tggtatactg aaaaaaatcg
    28321 cctgcaaggt caaagttttt agaataggga tttgctgacc aagcatcaaa aaaggggagt
    28381 cgtgaaatat cttgcaatgg agcttctttt tcataacttg cttttggcat aagaagcggg
    28441 aaaacggtga tagcaagaga agaggctata atgagagatt ctttttttaa tgaaatatca
    28501 aaaagaaggc tttcttcctc ttttgcaagg actggctgaa ttattgctat aaaataaaag
    28561 cctaagataa aaagaaacaa tcctctttgt cttaagcttt tacatgtaca tttcattata
    28621 atagtaccta gaattatttt atttttgtca gttctacatc aaagacaatg taggcatttc
    28681 caggaataac cccagctgca cctgctgaac cataggccat tgaaggtggt aaaacaattg
    28741 ttcgtttttc tccaagcctc atatcttgca ccattacatc aaagccagga atcatctgtc
    28801 ctgctccagc aacaaattct aagggagctc ttccttctga ggaatcaaaa acgctatcgt
    28861 tgagcaaata accagtataa tgcaccgcaa cttctttgtt tgcgccacat tttacaccgc
    28921 ttccttcgct ttttatacca aaataaatac cttctgctgt tttttcaaaa cctacaaact
    28981 tcttttctat tagtccaatc gtttctgcca ttgctttttc aagttttgcg gtattttgtt
    29041 ttactatttc cacagaaagc ctatcaaaat catcttgtgt tgctgtaaaa gcctttgctt
    29101 tttcgccctg acgaattatt tttatagatt ttatttcatc accttgtttt attgtgtcga
    29161 caactttttg accatgaagc actttaccaa aaacagtatg ttttccatcg agccaaggtg
    29221 ttgccacgtg ggtgataaaa aattgacttc cgttggtatt tgctccagaa tttgccattg
    29281 aaaggatgcc aggaccggta tgcttcaagc tatcgtcaaa ttcatctgca aatttatagc
    29341 cagggccacc tgttccgttt cccttaggat cgccaccttg aatcataaaa tcagctataa
    29401 cgcgatgaaa ctttaaacca tcataaaagt tttttccacc tgttgccttg aggctacctt
    29461 ctgccaaacc tacaaaattt gtaacagtaa gtggtgtttt ttcataaaaa agttcagcaa
    29521 caataagacc tcttgtcgta tctattgcag caaaaactcc atcttttcct tcaattgctt
    29581 tcaatccttc atccataggg ctacatcctc caaacgatat taaaattgta aaaagtaata
    29641 ctaaatgtat tgtttttttc attatttatc cctcatattg tattttaaac cattcataaa
    29701 tagcatcaac cctatttttt aatgattttt ctattgtttc atttaagaac gcaacatcta
    29761 aataggtcgc ttgtactaaa aaatatacta aaatatgctc ttcaaaagta tgcgtatata
    29821 aaaataaatg taaattatct tcttgtattg ctttaaataa aagatatttt aaagggtcca
    29881 ggtttttcat atacattgca atttcagcat ttttttgaaa ataagcaaca tccattacat
    29941 aagaaccaaa agtcgtatcc gtttgacgaa caagaatctg caaatcgtct atggaacttg
    30001 gtttaggatc ttcttgtctt tcttgatttt cataactttc tataacaaaa cagtcttcgt
    30061 atagtatttc tttgcgtttt tttctttcag aatagtattc cattcccttc attttggaaa
    30121 aagcggttaa aatagaagcc gttttagcta tctcatctgt atgtgagatg aaatttttct
    30181 cgttttttgg ttttgtatat aaaaataatc cttcaacaac aatcaatggg tctatatcat
    30241 gactttcatg ccattttttt ttcgcttcat ctatgatagt cgccttagga gcaagacttt
    30301 ttagtttttc tttttttcta tacgaaatag aaactaaaga ttcgttttta atcaattctg
    30361 cataggtttc atcatctaaa agaaaacgaa agccttcttt tttaggctct gcattgattt
    30421 ctcctacaaa ccaaaaactg agaataaaaa gaaaaagaat tgattttttc atacctagaa
    30481 aaatcctttg tattttaccc gtacctgttt aaataaacct tcgccttcgc tttctgtctc
    30541 aatatcactg atatcgttta aggttgtatg tacaatacgt ctttcaaaag gattcatcgg
    30601 atcaagcaag aaagaatttc ttgacgtccg caccctatct gcagtggtat acgcaagacg
    30661 aaccaaactt tcttcacggc gaattcggta attttctata tctaagataa ttctctcatc
    30721 acttcgccct agttttcccg catacacatt tgtaagcaac tgaagcgcat caagatttcg
    30781 gccttttttc cctataataa cagcagaact tgacgattct aggcgaagtt ctattttttt
    30841 ctcttctcga tgcattacat caacagaaat atcatagccc atctttgtaa caactgtcgt
    30901 aatgaaagtt acaatttctt tttcaaaatc atcttgggga aggggatttc caaagacttt
    30961 tttctgtgtt tttttagcag cttcaacagg aatatcacta gaaatgtcac tgacgatttt
    31021 ttttccatct gtgtgtacac ggattttcac aaagcctttt ttaaaaagag aatttttttg
    31081 tgtttccata atttctacat caaattcttc tctttctaaa cctagttctt gaacagcaag
    31141 ttcaattgct tctttttcgg ttttaccctc aaattcaaat atcatataaa tacctcacgg
    31201 ttaaaatttt tcttcttttc aaaaataaaa ctaggccaat agccgagctt tattttttct
    31261 tttttggcga aaaagcagga attatatttc cttcaatttc tgcttttttt tctttcatca
    31321 ttttatttat agcaagctgt tgcaccaatt gcagtaagtt actaattgtc caataaagca
    31381 atagacctgc tggtgcattg tagaagataa aaaagaaaaa gagaggcata cccattgtca
    31441 ttattttcat ttgcgttgca tttgccccag cggcagcagt actttgggta atttttccaa
    31501 acaataattg cgaaattaca taaatcacag gcaataatcg aaggtgattt cccaaaaatg
    31561 gcaaggaaaa tcctaaaaca tatagagaat cgccttgaga aagatctgga atccatccag
    31621 ggataaacat agctcctcta aattcaaaat aattgttaaa tagattgaac atagcaataa
    31681 tcaaaggaaa ttgtatgagc aagggaaggc aacccatcat gggattatag cccgtttcct
    31741 tatagagttt tgcaagctcg atattaagtt tttctggttt tcctttatat ttctcttgaa
    31801 tttctgtcat taaaggctgt atctcttgca ttttcaaggt agaaacagag ctttttttgg
    31861 taagcggaaa aagcgcaact ttcaaaagta tcgtcataag aataatcgaa acgccccagt
    31921 ttggaataat cttataaaaa acttgcataa tcgctttcat aaaaatttca agccaagata
    31981 gaattccaga agattgcaag ctatcatcaa aacggatatt tgaaagtccc cagttatttt
    32041 ctgataaaag attatatttt tttaagtcct tatctgttcg tggccccaca taaatataat
    32101 aggtatcttt attgtttttt tgagaaatag catttcgagt caagaaaact tgcgcattcg
    32161 tatagtcatt tttttctatt tttgtggaat acagcgaggg tcccattgtt tcaggatttt
    32221 gtggatgcac taaaatagta aaatatttac ccgcaattcc tgtccatgtg tagggattgc
    32281 tatattcttt tgttgcatta tctcccaaaa gctgtttttt tcttttatct tttgtatatg
    32341 acataaaggt tctattttca tagcgatcct tttttttgtc ataatatggc cctatttgcg
    32401 gagatgtacg cagagtatag gcttttccat ttagatttaa ttcagaaaat gcagaattgc
    32461 tattttctaa tccgtctata attacatcaa gctgaaataa ataatcatct ggatgaaagg
    32521 tatattgttt tattaaagta aaggtatcaa aagagccgtc ggcctttgca agtcgaaatt
    32581 ttttataaaa gccataggta tagtcattta ttttatttac ttgaaaaaga tcatcgataa
    32641 ttttgctatt ataggaacca aaggaaaggg aaaacgcacg atttatttca gaaattgaat
    32701 cggccatttg cacgcctttg tcgccgtcca agtgattttt tagctcaaaa tcgacaatat
    32761 ctccaccacg gtttgaaaga gtaatcttcg cattattcgt ttctataaga aacttttctt
    32821 ccgaaataga aacttcttca gcaaaatctt ctaaatgcac cattttttca agattatttt
    32881 gtgcattttc ttcgtttgca atgaagggtc gatcttctag atcccttact acttcttcaa
    32941 caggataatc tacaggggga tttaaatatg tttgcaaaaa cataaagcca atcaatacca
    33001 gagatgataa aacaattgcc aaaatggtat ttttttccat ttttactcct aatacttacg
    33061 gaactggatc atagccccct tcgtgaaatg gatgacagcg tgaaattctc ttaattccca
    33121 aaaacaatcc cttaaaagga ccatgttttt ttatagcttg aagagtataa ttagagcatg
    33181 tgggctgaaa tctacacgtt gcaggaaaaa gaggcgaaat acatttttga taaaagcgaa
    33241 ttaaaaaaat aaaaatacga gaacatattt tttttagcat aataaccctg cttttttaca
    33301 taatagccgt atttgatttt ctcgaatact aattgtatca tttcctggat agaataaaaa
    33361 caataaatca taggatcggg ctagttcacc ctttaagttt cgatacactt ctcggcttat
    33421 tcgtttcgca aaatttcttt gtacagctgt accataaccg cgaggtagtg caaaggcaac
    33481 tctattgaag gctaaatctg aaggtaaaaa aaagagcttt gcacctcgaa cgctcatcct
    33541 cttgcctgtt ttaaaaagat tttgaatctc ttttttttgt tttactcgtt ctttttttgt
    33601 aaaaccccgg ctcttagaca agcgcatcct tccctaataa aaattaatat ggtttctttt
    33661 catcagaaac agataattta tagcgtcctt ttgctcttct tcgttttaaa ataagttttc
    33721 caccacgagt tttcattctt gcgcgaaaac cgaattttct atttcttttg actttacttg
    33781 gttgatatgt ccttttcatg gaacatctcc tttatttcta agaaagtttc ctttctaaat
    33841 ttttattttt tgtagataca ggtttctgag tttagcatat ttattatttt ttggtcaagc
    33901 tctcttattt tttttggaaa tatttttcaa tttttctagc ttttctttca aatcctctgg
    33961 caaatcttct tttatttgta tttctttttg tatttcttct ctttcttctt ctttttttgg
    34021 cgcttcaggt atatttttta taccaaaatc gctgccttta aggcgaaacg caatactcgt
    34081 aatttcaaga gcagggattt ttttcttcaa gccacgtaaa atatagctat tataaaattg
    34141 caaggtctga atccaacctg aatgatctac ttcaaccaat aaaaccttgt ttacgaggtc
    34201 tattattttt gtatgcgaat aaagtttttc ccctaaatat atattttctt caaaacctgt
    34261 tttttttggt cctcgaatag agcaaataat gtctttccat acgtttcgaa tactaacagc
    34321 attttctacc atttcagaat ctacgccgtc catgtatttt gctaaaatag tggatatact
    34381 agaaattgca tcatctttca tcccaatgtc ctttttgtat agtatacacc tttgttttag
    34441 attttacata tttttcatag ggttcaccag gtaaaaaagt cgcaaaaagc tgttcatact
    34501 caggcaataa atgcattatt ttttcccgtt ttcctggatc cagttccaat aacacatcat
    34561 ccataagtaa aataggtttt ttatggttta cttcaaaaat atactttgct tgtaaaatac
    34621 gcagtaaaac cgataacaaa cgtttttgtc ccgtagatgc agttaaaaca aaattttccc
    34681 catcttttat aaaaataatt ctatccctat gtggacctga aacacttgtg ccgagatttt
    34741 tttcaattcc ttcttttgcc tttaaatgca cctctatttc gtctttattc atttttttcc
    34801 acgatgaatg atatgaaaga cttaatccag aaataccgct tatttcttcg tataaagaac
    34861 ttaaatcgcg gttaaaaaaa agacttatat cttctcgttt ttttcgtatt tccataccta
    34921 tctgtataag attttctgta aaaacaggca ataaatcaaa attttgttct tttagtacaa
    34981 tatttcgcga gcgaaggatt tttttatatt ttcgcaagct atcaacatag atattattgt
    35041 gtaagcttat ggactggtct aaaaaaaagc gccttttttc tggctctcct gttacaaagt
    35101 ctaaatcttc atgacaaaac acaatgcaag gaatcgcatt tatcaactct tttctgtctg
    35161 ttattttttt tccgtttttg agaatatgtt ttttaccatt ttcaaatttt atcacaattg
    35221 aatgtgtaat ttcatgagaa tctttgtata aagcttttat actataggca gaatgcggtt
    35281 tttttataat ttctacatcg ttttttgtac gaaaagatga accgtaggag caaatatata
    35341 aggcttctaa aagatttgtt tttccttgcc cattatcacc tttaaaaaaa acttcttttg
    35401 ataataaatc aatactcgca ttttctatat ttctataatc tacaaaagat aaagataaaa
    35461 agggcaaaaa taattactcc atttgcatag gcataacaat gtggaaaaaa tctttttctg
    35521 gttcagattt tactgttaca gctttcatag aagaagtaaa atcaaaacgt attcgttcgg
    35581 tattgattac tttcataggt tcttcaatgt aaatataatt aaaggccatt gttatatctt
    35641 ctccgctata tttacaggga atttcttctt ttgcacttcc tatatccgat tcttgagagc
    35701 tgattgttaa caagccatca gaaagagcaa aataaactct atgtgatttt tgctcaacga
    35761 gcagtccaac acgttttaat gcatcgccta agtctttttt atctacttca aaagtgaaat
    35821 cttgtttttc tggtataact ctttcgtagt ttggaaactg tccttcaatt aaaacagaag
    35881 taaaaatata gtttccaaat tttacaataa tcatattgtc aacgaaggca agagaaatac
    35941 taccttcatt tggtgcacgt tttaaaataa tgtttagaat ttttggtggt acaattgctg
    36001 gagaaaattc ttgtactcct tgataaagag cttttgaaat atatgagaga cgtctaccat
    36061 cagttgcaac taagattaaa gcatcatctt ttttatcaaa atatacaccg ttcataaaat
    36121 atcgtgtttc gtcatctgaa acagcaaata ttgtctgtgt aatcatatct tttaagtcct
    36181 gaacaggaac ttctataaag tttacttcat cagtatttat aaattctgga aatttatcgc
    36241 ttgccatgct ttttaactga aattttactt ttttggcaat tggctttatg gtaatttttg
    36301 tttcatcttg tataaattct acttccccag aaggaagaga agttaaaatg ctcataagtt
    36361 tatcgcaaaa tatagttgtt tttccttccg tttcaatttc aataggaatt tttgtttcaa
    36421 aatttacttt tatatctgtt gctttaatag tgagagtatt attttctgca ataaataata
    36481 cgttagaaag tatagacaat gcattttttg ttgcaattat ttcttgtgca atgcttattt
    36541 ctttaagcat tgtatcttta tcaaaagtga atttcatgat tcctccaaaa taccttatta
    36601 ttatatatat ataaataata gtaatagtag taatagaccc tgttaatatc ttaaaaacct
    36661 agttaattct ttatagtgta aggttttatt ttgtgtataa gtataaaatt ataagattta
    36721 cttatgcaca tattcctttt attgattagt catacacata gaataacaag attgtaacat
    36781 attatgcaca acttgtctaa gctatttttt tgcgtctttt atggtattta tcagcaaatg
    36841 aatgttcgat tgtagatggg tatctgtttt tacgagctct tctatttttt gacatgcatg
    36901 cataacggtt gtatgatctc gaccaccaaa ctcagctcct acttctgttg ttgaaaattc
    36961 ggtaatttct cgtgctatat acatagctat ttgtcgtgct gtagcaatgt tttttgttct
    37021 ttttcgcgct tttaagtccg taaatgagag gttataatga tcggctacaa ttttttgaat
    37081 tttttctatg gaaatattgc cttgtcgtgg agatgtaaag gcgtcgcgta attgttgttt
    37141 tgcgatttcc aaggtaattg tttttccgat gagttctgta taggcaataa tttttgtaat
    37201 agctgcttct aaatcacgta cgtttgaaac aacgttttct gcgattaaat ctataatatc
    37261 atcagaaatc tttttattca tgagttccag ttttttaact atgattgcac gtcgtgtttc
    37321 gtattgggga ggagaaaggt ctacagttaa tccgcgttca aaacgagatc gaagtctatc
    37381 gcttaagttt cgaatttcgc ttaagggtct atcgcaggta aaaaccattt gtttaaaggc
    37441 gtcgtggaga acattgaagg tgtgaaaaag ctcttcttgt gtttcagttt tattttgtaa
    37501 aaagtgtata tcatcaatca ataaaacatc agcattgcga tatttatttt tgaattttgg
    37561 aatagttcta tcatttaatg cttgtataaa ttcatttgta aagctttctg cggtaagata
    37621 aattgttttt aagtcagtat tttgatgggt atagtttcca attgcttgca ttatatgagt
    37681 tttaccaaga ccaactcctc catatataag aagggggtta tatgcttttc cagggttttt
    37741 tgatatggcg cttgcagcgt tgaaggcaaa ggtattatta tcgccgatta taaagttgtc
    37801 aaatatataa tcttctcgta attgcggatg ttttcccttg ctgttttgta ctggtttttg
    37861 tacaatttct tttttttcta tttttatatc atttgtaaga ttgtgacttt gtatattttc
    37921 ttctttttta gcttgtataa aaaattcaat agtaatctgc tttccggata attcttcgag
    37981 tttttgttct aaaagagcaa tatagccgcg agaaatgagt tggtctttga aaaagtttga
    38041 ggggaccata attgttatac tattttctgt tgaatcgtgg tattcaaaag aaaaccacat
    38101 gtcaaattct tttgcttttc cttcttgtat ataactttct ttaatttgtg agagcgcttc
    38161 ttgaaaaaag attgaataat tccatttgtc cataaggtcc attataaact gtctttactt
    38221 ttttccgcaa tacagttata ctgaaagtcc aacatttatt gtacttttat aaaaaataca
    38281 taggattttt gtttttatta ctatggttta ctaaaacaca tacctcaatg agaaactttc
    38341 gaggcactga aaattaccga gttctagttt atagtgtctg ttcaaatcaa tatatattta
    38401 aggaagtaaa atgaaccctt catattccgc atctaatatt caagttttaa aagggcttga
    38461 agctgttaga aaaagacctg gtatgtacat tggttctacc gggcctgatg gcttacatca
    38521 tctcgtgtac gaggttgttg ataactcaat tgacgaggca atggctgggc attgtgatcg
    38581 tatacttatt gttttagaaa aaaatgatat ttgccgcata gaagataatg gtcgtggaat
    38641 tcctgttgat attcacccaa ctgagggtgt cagtgcctta gagcttgttt taacgcgctt
    38701 acatgcaggg ggaaaatttg ataagggttc gtataaggtt tctggcggtt tgcatggtgt
    38761 aggtgttgct tgtgtaaatg ccttatccag ttggcttgaa gcgacaattt gcaaagatgg
    38821 cattcagtat catcagcgct atgaagtggg agttccttta ggaccggtta aagaagttgg
    38881 aaaaacagat aaatcgggga cgataattcg atggaaggct gacccgacag tatttgaaga
    38941 aacgcaaacc tataactttg aagttttggc gagtcgctta cgggaacttg cttttttaaa
    39001 tagtggtata tcgattgttt ttcgtgatga gcgtttaaca acaccaaaag aacaggtttt
    39061 ttcttttgaa ggcggtatat cgtcttttgt aagttatctt aatgaaaata aaatttgtat
    39121 tcaccaaaca ccggtctata tttcaggaga acgagatgag attgttgtag aaattgcctt
    39181 gcagtataac gatagttttt ctgaaatttt gttttcttat gtaaatgata taaatacaaa
    39241 agagggtgga acacatcttg acggttttaa aacttcttta acccgtgttt taaatgattt
    39301 tctaaagaaa aatccaaaaa ttcaaaaaaa gatggataag gaagatcgtt ttagtggcga
    39361 tgatgttcgc gaaggtttaa cagctgtttt gtcggttaag gtgcctgaac cgcagtttga
    39421 agggcaaaca aaaacaaagc ttgggaatac tgaggtgcgt ggtatcgtag attcttttgc
    39481 aacggagcaa ttgactttat tctttgaaca aaatccttct gttatagaaa aaattcttga
    39541 aaaggcggtt cttgcagcaa aagcaagaat agctgctttt aatgcacggc aagcaacaag
    39601 acgaaaaaat gctcttgaag gtggcgggct tccggggaaa ctttgggatt gtatagaaaa
    39661 agatccagca aagactgaag tgtatatcgt ggaaggggac tctgcagggg gcgcagcaaa
    39721 aaaagggcgt gatgcaaaaa ctcaggctat tttacccttg tggggtaaga tgctcaatgt
    39781 tgaaaaaaca cgtatagaaa aagtaataaa taatgaaaaa ttacagccag ttattcaaac
    39841 gcttggagca ggtataggac aaaactttga tacttcaaaa ctgcgctatc ataagattgt
    39901 tattatggct gacgcggatg ttgacggttc tcatattcga acgcttttat tgaccttttt
    39961 ttatcgctac atgactgcat taattgaaga aggttttgtg tacttggcaa tgcctccttt
    40021 gtataaactt tcctataaca aagaagagcg ctatgtctat aacgatgaag agcgtgaaaa
    40081 agaaaaactt gaaataatgg aaaaatttaa tgttaaagat gaagata
//
LOCUS       DAOSSW010000007        38594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_1171_length_38594_cov_6.832144, whole genome shotgun sequence.
ACCESSION   DAOSSW010000007 DAOSSW010000000
VERSION     DAOSSW010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 38594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38594
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1171_length_38594_cov_6.832144"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            439..1032
                     /locus_tag="PKW26_01360"
     CDS             439..1032
                     /locus_tag="PKW26_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOQ92249.1"
                     /translation="MDILLEYCATEIIVKKSRFIAELFPIETQAEARDVLKNQKQKYA
                     DAAHVVHAFVCGLEGQISGMSDDGEPSGTAGRPAYDVLKGRNLTNLILTITRYFGGTL
                     LGTGGLVKAYGDAAREVLDKAKTECYIPKISFCFKAPYEVYEGIKRSIAHFPLTNAKE
                     EFLERVGFQAEIPLEHKENLSAIIKDVSKAMVTVEFL"
     gene            complement(1060..1461)
                     /locus_tag="PKW26_01365"
     CDS             complement(1060..1461)
                     /locus_tag="PKW26_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92250.1"
                     /translation="MKKHFLFFAFIMIIAIAFTACASTKQLENADNQQETSQALDEIR
                     DHSFLEGTVWKAPIKIMDFDAFQRLEIKNGQAWFATEMGNAVDGGDVPERIELRGNTI
                     RFISADGVGIGTLDEDTIIMKHDGLIYTRVK"
     gene            complement(1522..2967)
                     /gene="asnS"
                     /locus_tag="PKW26_01370"
     CDS             complement(1522..2967)
                     /gene="asnS"
                     /locus_tag="PKW26_01370"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOQ92251.1"
                     /translation="MKTELIKDILVSSPDKREICVQGWVRTKRDSKNLCFIQVNDGSC
                     FTSMQVTFDRENTNVDTDAIEKALEKVTTGASIEAVGTLVPSPASGQAVELALSSIRV
                     LGEAPTESYPLQKKGHSMEFLREIAHLRPRTNLFGAVARMRSQMSYAIHTFFQERGFH
                     YVHTPIITASDAEGAGEMFQVTTLNLEELARTGIKAGKGGKSEDEADMLVDYNKDFFG
                     KHASLTVSGQLEAECYATALSRVYTFGPTFRAENSNTTRHLSEFWMIEPEMAFFKNED
                     NMQLAEDFIKYLLSWALEKCSEDMQFFEDRVQKGIIETLRHVVSSDFIRLSYTEAIAV
                     LEKNADKFEYKPYWGCDLQSEHERYLTETVYKKPVIVTDYPKDIKAFYMKLNDDGKTV
                     RAMDVLVPGLGEIIGGSEREADYDILLEKIKAMKLDPKDYWWYLDLRKYGSVPHSGFG
                     LGFERLLLYITGLKNIRDVIPFPRTPKSAEF"
     gene            3074..3147
                     /locus_tag="PKW26_01375"
     tRNA            3074..3147
                     /locus_tag="PKW26_01375"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3108..3110,aa:Asp,seq:gtc)
     gene            3364..5019
                     /locus_tag="PKW26_01380"
     CDS             3364..5019
                     /locus_tag="PKW26_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOQ92252.1"
                     /translation="MANTELLDLTISEMLREQTKKNPNQDFMVYSDRDLRFTYAAFDA
                     RVDALACALLAIGLKKGDHLGIWATNVPDWLSYMFAATRIGVVTVTINTSYKLHELEY
                     VIKQSDIRMLCISDGMKDSQYVSMIKELIPELDTCQRGALVSEKFPELKQVVFIGPEK
                     YRGLYSTHELLLLGQHIDIARVHEVEQGLNCHEVINMQYTSGTTGFPKGVMLSHHNIL
                     NNGYFIGQCMKYSEKEKVCLPVPLFHCFGIVLGVMAVLSHGATHVMIESFDPLLVLAS
                     VHKEKCTSLYGVPTMFIAELNHPMFNMFDMSSLRTGIMAGSLCPIDVMKAVMSKMNLT
                     EITSVYGLTETSPGMTQSRTDDPMEIRATTVGKELPHIEVKVLDPETGEECPPGVQGE
                     MCCRGYNVMKGYYKNPEATAEIIDANGFLHSGDLGIKDEAGYFKITGRIKDMIIRGGE
                     NIYPKEIEEFLRQMSEIKDIQVAGVKDKRLGEITGAFIILHEGKTLTEEDVIEYCRGK
                     IAKYKFPQLVLFVDSFPMTASGKIQKFKLSEIGEAHRKSLGIE"
     gene            5082..5639
                     /locus_tag="PKW26_01385"
     CDS             5082..5639
                     /locus_tag="PKW26_01385"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOQ92253.1"
                     /translation="MKGNGLSMEIAQRLKGLRDIMELSTEDVASVCGISQEEYEKYES
                     GTVDIPVSILHSIATAYKFEITTLISGEEPRMHSYFLTKKDKGLGVDRRANYKYESLA
                     HGFINRKADPYIVTLTPEQTREMHFNSHPGQEFNYMLEGSMKIVIDGKELILDAGDSI
                     YFDANKKHAMQALNEKNAKFLAIIF"
     gene            5652..7340
                     /locus_tag="PKW26_01390"
     CDS             5652..7340
                     /locus_tag="PKW26_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOQ92254.1"
                     /translation="MLTKYVPKIDFSSSQDFRDNYTVIVPERFNFSYDVLDRLAMETP
                     DERALVWCNDEGEEKIFSYSEIKTYSEKAASFFQAQGIRRGEKVMLILKRRYEFWFAL
                     LGLHRIGAIAVPATHMLTKDDLVYRNNAASIKMIVAAYDEKVMEQIEASLEESKSVEL
                     LVSVSGISGLYENSFYNKKRDGWIDFNSGLEEAKDFIAPKREEVNDNEDIMLLYFTSG
                     TTNHPKMVAHDYTYPLGHIVTAKYWQCVRKGGLHLTVADTGWAKSVWGKLYGQWLCEC
                     AVFVYDYSAKFKPVDLLAQIEKYKITSFCAPPTIYRFLIQADMSKYNLSSLEECCTAG
                     EPLSEEVYNRFKAQTGHGLLEGFGQTETTLSLLNFKGESVKPGSIGKPAPFYNIKLLK
                     DDLSECVDGEIGEICFPLDKGSFPGLFKEYYRDAEKTREVMHDGYYFTGDTAWRDEDG
                     FYWFVGRFDDIIKCSGYRIGTFEVESILMQHPAIVECAVTGFPDEVRGQVVKATIVLS
                     EKYKPGSDVLAKEIQDFVKKATAPYKYPRIVEFVEELPKTISGKIKRRDIRAKD"
     gene            7343..8233
                     /gene="ispH"
                     /locus_tag="PKW26_01395"
     CDS             7343..8233
                     /gene="ispH"
                     /locus_tag="PKW26_01395"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOQ92255.1"
                     /translation="MAKVLRSKVLGFCMGVKRAVDMAEEVLNENEGKEVFMLGPLIHN
                     EQALQALYDKGLHLLSEEYIEERCNEKSIVIIRAHGTSPSILTRIEKTGARIVDTSCP
                     RVKASQKRAASYSQDGFLVFIAGDLGHGEVLSISSYAGDKAIVFKDAEEAKTFFKTSE
                     LFQKNPRAFDKAILISQTTIRKEEYDAIIAVLQKKLPQIEVFNTICSATKERQLFLRE
                     LAHEVDGILVIGGRHSANTQRLLSLAQELSPHAALVEAAHEIPPEFFRLEKVGLTAGA
                     STANKSIDEVEQKLFSMNEE"
     gene            8344..9603
                     /locus_tag="PKW26_01400"
     CDS             8344..9603
                     /locus_tag="PKW26_01400"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOQ92256.1"
                     /translation="MIKTIKDIDLSGKRVIMRVDFNVPMKDGVVQDDTRIQAALPSIQ
                     YILEQNVRSLVLMSHLGDPKKDSKKAQEKAEKDGKPFDKEAYIAGKHRMKPVADYLSK
                     LIGKEVVFAPSCLGQKAAVDALAEGDVMMLENTRFESAETAKDLAEQEAMAKELASYG
                     DVYVNDAFGTAHRAHASTATIAQFIDVKVGGFLMEKEVQYLEPMVSNPPKPMIAIIGG
                     AKVSSKIAVLESLLKNASALIIGGGMAYTFLKAQGYTVGKSLVEDDFIDTAKSLLAAA
                     KEKNVSIILPEDHLCAETFSPEAKAIAVDGQDIADNLMGMDVGPKTIAKYTDAILNAK
                     SIVWNGPVGVFEFDAFAKGTNTVADLVAQATAKGAISVVGGGDSVAAVNKAKLADKMS
                     HVSTGGGASLEFLEGKTLPGIAVLATK"
     gene            9634..10383
                     /gene="tpiA"
                     /locus_tag="PKW26_01405"
     CDS             9634..10383
                     /gene="tpiA"
                     /locus_tag="PKW26_01405"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOQ92257.1"
                     /translation="MRQKYIAGNWKMNKTKGEALELAKGLVAALKDGKNKYLIAPSFT
                     NLDAVAQVVKGSNIRLGAQNMSTDESGAHTGEVSVLMLKDLGVETVILGHSERRTIYK
                     EDDAMINKKVKLALQHGLEVILCIGELLEEREAGKAEAVCKAQTEKGLAGLSKEDMKN
                     VVIAYEPVWAIGTGKTATPEDAQAIHAYVRDVLASLFDKEVADATIIQYGGSMNAGNA
                     KDLLAQKDIDGGLIGGAALKVDTFEAICKAY"
     gene            10531..10902
                     /gene="secG"
                     /locus_tag="PKW26_01410"
     CDS             10531..10902
                     /gene="secG"
                     /locus_tag="PKW26_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOQ92258.1"
                     /translation="MGVLGIILLIAFIIVCLLLVAIVLLQNEEGDGLGGLLGGASNVA
                     FGSRAGNVLTRTTYVLVTLFFALSIGLALLNKAPSVDAKGLEDAARAQDASGTESEYW
                     LEETSSDNPAPSLLESADTDF"
     gene            10916..11395
                     /locus_tag="PKW26_01415"
     CDS             10916..11395
                     /locus_tag="PKW26_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOQ92259.1"
                     /translation="MFLGKIFNPKAIKLNLESDFKDEVFEELIEVLVSSEPSLNRNEI
                     LFALQERERLMSTGIKKGVALPHCKIKGIQGVRGAIGVSQKGIEYNALDLNPVHLVFM
                     IVASSEDSLGYLQVLKQLALILNERAFLQGILEQNTAVDVHELIKSYEKKMAEKKII"
     gene            11406..11714
                     /locus_tag="PKW26_01420"
     CDS             11406..11714
                     /locus_tag="PKW26_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92260.1"
                     /translation="MAGADVISHLIEIEKRAAEFMHQAHVEADARLTKARTEVDSQFK
                     EAYEKLMKELEADYTKKSIALKEQYEKDFNDFVREITETPQDTEAIRTLFLELLDKRL
                     "
     gene            11722..12726
                     /locus_tag="PKW26_01425"
     CDS             11722..12726
                     /locus_tag="PKW26_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOQ92261.1"
                     /translation="MDKSAAHAFVFAKASGMLSKSFVGNRFLSIFQEKKLQDLWKLLF
                     EDEVPLLPEYLLAQTIEKKAEEVFVSDFTQLLSYYAKPDPVLVQLLQVYEYNNLKLIS
                     SALMNNKTQMPEIIDLQSFSILDYSKWPDIKKMTENTVFAWYDTIPKVKELKTRDHAL
                     DVQYIRSLWTSVQRLPAAERAPVQAFIKEDIVIQNILWALRLKYYYGYDAEAIRPFLA
                     GLKDNPDEKDILAGPAVQILDKEIDSFADWKDWEYRRFLNRHEPDEIWSIDPKWVAQA
                     AEGYLTKKALKNFRKYPFTAMVLVCWFKVKQAEVSYVRTAVQALRLNVSEEELSEYMT
                     "
     gene            12741..14741
                     /locus_tag="PKW26_01430"
     CDS             12741..14741
                     /locus_tag="PKW26_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOQ92262.1"
                     /translation="MARTVSMQLIELMIHKRDISFVLEYLGKKGDFQFQSHIDDEKAA
                     ETSEESRAFDSLQDIRSYLHIEDLETFVEGASLPTEGEAARVKELSASVRDLREREIG
                     AKEAKQRLEETYQEALSFSNLKLPYSELEYLSFLSIRIGKIDPAAIDSLSFSLGDRGL
                     IIPLGEDKTRILAAASKKGRFALDAELKRAGFVPLEIPENFQGIPDDVIVGMKEKLDV
                     AAKELEAIETEKRNFAQLHTDEIYTLLSSYSLAMQVASMQSRLEATQYVYRITGWIPK
                     SHMRGFTSDLDNLSEGRIAIRQYQPEEVPSVRAGQEKVPVKLQHGKFVSAFERMVFSY
                     GAPLYGTIDPTPIVAFFFTLLFGIMFGDAGQGLVFLLIGILMTVGVIKKFPVDPKFGA
                     IFIAIGCSSTIMGLLTGEFFANEHVLIPFGRFVTGLFGESQDRVLHLMPAADSLDKLF
                     YFFLFTIANGFIINSIGLIINIVNNFLLKKPEKAVFGKTGMFGLLFYWYIAFVIIRIF
                     AFKSTFMLYDWIAIGITLFGVFFAHPLERLFTKQKPIFPLGIGTAIIEGLVEILEVLS
                     SYLSNSVSFLRVGAFALAHAVLGYIIFSMTALVQSSGTLFGIVGGVLVSIVGNLIVIV
                     LEGMIVAIQVVRLQYYEFFSKFFSEAGREFEPFVFVYKGAKG"
     gene            14837..15142
                     /locus_tag="PKW26_01435"
     CDS             14837..15142
                     /locus_tag="PKW26_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HOQ92263.1"
                     /translation="MKKKIAVLMFCMLLLSSAMLFAEAETVQNVTDFSWVKYLAAALA
                     VGFACVAGGMAVAKIGAAAMGAMSENAELSGKALPFVGLAEGICLWGFLVGLLILIL"
     gene            15161..15499
                     /locus_tag="PKW26_01440"
     CDS             15161..15499
                     /locus_tag="PKW26_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOQ92264.1"
                     /translation="MQYFFIGERELVLAFSLVGVAGETVSNRTEMLEVFERLTGKGSR
                     DLHAPVLSERPKILILTEEAASMIEDEIRQWQMKGSFPLIVEIPGLHGHIEGKKSLTD
                     AIREAIGIHI"
     gene            15511..16155
                     /locus_tag="PKW26_01445"
     CDS             15511..16155
                     /locus_tag="PKW26_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92265.1"
                     /translation="MEELRSTELLDKEILEDARVKAEHILQSADKEAEKILSSVSQKI
                     DALRQEKELVFQNKLAYFKKDLDAKIPLKKQRFKVSFQTESVRKQMYTHIMSLPLEEK
                     NKVYESMLRSYSDVIAEPSFIVKECYYDKDALVSIVTNVFGKKSIDIESLKVCETGIL
                     HKNSVEGFKPEGFVLEAKDGSIVCRVMLEELIEGLIDTYSFELVDALFSGRLPE"
     gene            16152..17945
                     /locus_tag="PKW26_01450"
     CDS             16152..17945
                     /locus_tag="PKW26_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOQ92266.1"
                     /translation="MIEGKITRISGPIVYASGLDSCGLYDVVDVGEAKLIGEVIRQNK
                     GLATVQVYEDTTGMRVGEKVVSQQRALSVHLGPGLIGTIYDGIQRPLKEMYEKSGAFL
                     LPGERLEALDTEKKWTFNPNDDLKTEIDGSGKTLYKIEAGMSIGFVQETSSVKHSIMV
                     PPFIRGKRLVSFSPQGDYSINETIAKTELGEEIKLAHYWPVRKPRPFAEKLMVSEPLI
                     TGQRIIDVFFPLSKGGTAAIPGGFGTGKTMTQHAIAKWCDADIIVYIGCGERGNEMTD
                     VLTEFPSLIDPRTGRSLMERTILIANTSNMPVAAREVSLYSGITLAEYYRDMGLAVAI
                     MADSTSRWAEALRELSGRMEEMPAEEGFPAYLPTRLAEFYERAGRIISLSKKEGSVSI
                     IGAVSPPGGDFSEPVTQHTKRFIRCFWALDRDLANARHYPAIGWIESYSEYAEEVRSW
                     WDNLDPRWAEIRGKALDLLKREERLQQIVRLIGPDALPDSERLVLIVSEMIKNGFLQQ
                     NAFDDIDVFSVPEKQILIIDLMMQFYERSAALIKNGSPLLKISALSIREDIIRIKTSV
                     PNDALDEITKVRMKMDEEFSTIEHIYRKAVI"
     gene            17945..19330
                     /locus_tag="PKW26_01455"
     CDS             17945..19330
                     /locus_tag="PKW26_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOQ92267.1"
                     /translation="MKGVEYKGLKTVDGPIVIVKRSENSFFGEIVYVRDRLGEKKTGR
                     IIDISEDMAVVQVFGSTNGLDLDETSIEFLEEPMELRVGEGLLGRVFNGLGKPIDGYD
                     EIISSTKRNVNGLPLNPYAREYPRDFIQTGVSSIDGMNTLIRGQKLPIFSGNGLPHNR
                     LAAQIIRQAKILSADEEFVMVFAGMGIKYDVARFFVDTFEQSGVLSRVVLFQSLADAP
                     SIERIITPRCALTAAEYLAFEKNMHVLVVMTDMTNYCEALREISTTRGEVPGRKGYPG
                     YLYSDLAELYERAGKIKGSSGSITQIPILTMPNDDISHPIPDLTGYITEGQIVLDREM
                     YQKGIYPPVAGLPSLSRLMKDGIGEGMTRDDHKDVSSQLFAAYSKVKSIRNLAAIIGE
                     EELSPLDKQYLDFGNKLEMEFFSQKEEEDRSIAETLDAGWQMLSLLPRSELYRIDKKL
                     IDKYLGPEQLS"
     gene            19444..20067
                     /locus_tag="PKW26_01460"
     CDS             19444..20067
                     /locus_tag="PKW26_01460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOQ92268.1"
                     /translation="MARLNIAPTKSNLLVMNEQLAISQDGYELLEQKREILVMELMRM
                     VEQVKLLEQRINAQVEKAFPALRKMLLVTGVDTVERLSQAVQYDFQIHEKEMSAGGMS
                     FSSVSVALPKRELFYSFVDSFAETDAVMAEFFELLKLLTEMASIRTIAWRLATEVKKT
                     QRRVNALEKMVIPQTKETKEYIETVLEERERESIFVLKALKSKHGRS"
     gene            20154..20639
                     /locus_tag="PKW26_01465"
     CDS             20154..20639
                     /locus_tag="PKW26_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOQ92269.1"
                     /translation="MKHLFENVLIVINDSNASIQAAEFGILMSKFNGSALHAVYVIDT
                     ATLKQLTLQKIFVKEEYDEFSESLEADGKRYLQYVQELGKAKGVAIETELRSGSVWSE
                     VITYANEIEADVILLGGTTTSDPYQRDMFNSSYGEILLHSNCSVLYVKEEYVDQLYKL
                     L"
     gene            20654..21064
                     /locus_tag="PKW26_01470"
     CDS             20654..21064
                     /locus_tag="PKW26_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92270.1"
                     /translation="MRIAILHFLDDDKTSHKTILQNLQRTANAKGHEVSIFSAKKDAY
                     NLRLSIYDYISLIIPSSPVFGSKIPSRVSELLSSCGTISGKKAAALVLKSGFSSNKTA
                     RNLMRAMEREGLKLDYFDIVKNADHANYVGKKLG"
     gene            21070..21858
                     /locus_tag="PKW26_01475"
     CDS             21070..21858
                     /locus_tag="PKW26_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOQ92271.1"
                     /translation="MKNYYALLEIKPTASIAEIKKAFREKAKKLHPDLNASQHTKEFQ
                     LLVTAYETLSNAKTRSSFDWAFSQSEKQKQKHFDYRVWLLKRDDDESRVKLIIYDLLR
                     HREDEAVALYLNIKKENPFFCFSSFLGREAFMDLGYILAEELLFRKEYYDAFLLFERI
                     IKMECEKAYFKHFYPEVLKQVNAILRASFVSKLSDELAIDLYERAIDLPLDKKYGALC
                     FCKAGELYLKMGDMEAAKICFKEALLLDTSLTEKNTTKKFFETA"
     gene            21916..22662
                     /gene="map"
                     /locus_tag="PKW26_01480"
     CDS             21916..22662
                     /gene="map"
                     /locus_tag="PKW26_01480"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOQ92272.1"
                     /translation="MIHLKNESQIDGIRKSCKLLAQLFEELIPEVKAGMSTADIDALC
                     VDFITKHGGKPAWHQEGFPGAACISINEEVIHGLPSKKKFLKEGDIVSLDIGINLDGY
                     ISDATKTIAIGDISPEAKKLMEVTKKCLEEGIKACVVGNRIKDISLAVSKVAEKEGYG
                     IVTDFCGHGVGLAVHEEPSIPNKPQMGKNPRIQAGMVLAIEPMINLGTGDVEVLTDGW
                     TVVTKDRKISCHEEHTVAVFADRTEILSIL"
     gene            22771..24240
                     /locus_tag="PKW26_01485"
     CDS             22771..24240
                     /locus_tag="PKW26_01485"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOQ92273.1"
                     /translation="MKTWTKRFFYSLLFLIGILLFVSYSCSTSKTGSSKTAFADTSPE
                     VIIGKDNLAVLEAMQGAFRSIASQVLPSVVELDVVETRQRQQFSFGDLFDFFNRGEDS
                     KNGTQEYEASGMGSGVVVRKIANTYYVLTNNHVAGSAKQISIKLNDGRIFTGTLVGKD
                     ERKDIALVSFETKEDVRVAQLGDSDLVQQGDICFAMGTPLGYFSSITQGIVSATGRAG
                     TYVNNISDFIQTDAAINQGNSGGPLVNIYGEVIGINTWIASQSGGSQGLGFAIPINNI
                     KRAIDDFIATGEVTYGWMGVSLVEINDEYKEDLGVKGQNGAFLSSIFLTSPAAKAGMQ
                     AGDYVISLNGKAVKSVDQLVRDVGDIPVGKEAEFRFIRGKKEMTLKVKIEKRDEKMVS
                     DSSKLWPGFIANPITDEIRTRLSLDKKIAGVVISNLEAKSPAAAMRLQAGDIITAVND
                     KAVKNLAEFYAALDTVGQKEIWFDVYSNGHTLTTTRYKF"
     gene            24243..24884
                     /locus_tag="PKW26_01490"
     CDS             24243..24884
                     /locus_tag="PKW26_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HOQ92274.1"
                     /translation="MSETKHVNFFALEKACKEKGCPFCNLINERIYRYIDGMLFEHVS
                     DIPFRRAYRAAGGFCDRHGKILLHYRDGLAVAILGQNILSDYIEAFKKKKAPKFKACC
                     PACIEQEKIEKDFSSFLSENHKHKETQEQLTAYFTASDGLCVPHYAKLLSLFNPPTWL
                     KEFQEALFSSLNERALRFIDYSAWGNQDKFESLSREDQIVWQELARVLRGSVE"
     gene            25423..26631
                     /locus_tag="PKW26_01495"
     CDS             25423..26631
                     /locus_tag="PKW26_01495"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOQ92275.1"
                     /translation="MIDLAAFEKVIPQSSNDFSSHKSSIEDTKMCASKTASFIEEFNA
                     LEQSGALSEINLLRKENRELDKLVNDAAKLMSLTSVSDMLDYVISRFLDSFIPSFLGF
                     LIQPPRGQHIRQYCYKNLKQVNEKIPTDFYKTLKNYFFDSPSPIICSELPEKNFLCHH
                     KNNGLFFKPELIFPLRSIGGLYGVVVLSEKVVGGPYTSLEQLYIDRMMRFLAVGIQNG
                     LHHESSITDPKTGLYNYDYFMQRLEDEAYRIKRHGGLAALIMIDVDYFKNFNDSYGHM
                     AGDVALETLADVLRSIVREGDTIARFGGEEFVILLVESDEDYVLEVSDRIRDAIESTP
                     VMWEDQALSITASLGVRLISKNTLSDSRSLIVDVDKALYRSKEKGRNCVTFYTSSLLR
                     RATEYLENNV"
     gene            26772..27611
                     /locus_tag="PKW26_01500"
     CDS             26772..27611
                     /locus_tag="PKW26_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HOQ92276.1"
                     /translation="MQGDLPESLNAAALSGLMMNTPYYTDIGCLMETIDSCTTGASSY
                     TAAVNSALSSAVKYHRNFTSNGFDYYKYTTGLTIFHNIWEAQWLNIAYNPATYKAILQ
                     FGKNNAWGKYMQNMWNKTPPKPEIDIEADSYEPDGLDDDPVTNILGKGIANKQHHTFH
                     LLEGDIDVMAVNLEAGKTYTFMTEAGPLGADTYMILFDPSGAYIDEHDDIDYPNNLYS
                     KITYEAEKTGYHLLILVDEYQKYGDYYVYYDEGDFGPSLGKTFRKWTPDRKDKESYKI
                     SLP"
     gene            27672..28097
                     /locus_tag="PKW26_01505"
     CDS             27672..28097
                     /locus_tag="PKW26_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92277.1"
                     /translation="MKKILALFVLLVLFFSCISQKQNNAELKEVAVKEELLQLDEDIP
                     TSTAIDLDALSKEGIKIKGRVEKIVENEKTFSEVRYYVLITEKNTSLVLFNKKGFSEG
                     FENYLDKEVFIEAYKDIGMIGFKREKQVGLFVLSIREED"
     gene            28308..28922
                     /locus_tag="PKW26_01510"
     CDS             28308..28922
                     /locus_tag="PKW26_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOQ92278.1"
                     /translation="MYKEFLPYDKVRNNSLKMAARIHKEGFIPDIIYVSLRGGAYVAN
                     VISEYFKIVRKNDRPVLYASVVARSYSDIRQNDKVMVDGWTYSPEHLRHGDKILLVDD
                     IFDTGKTINHLVELIQQKGIPRNDIKVAVHDYKYFTYYPEQLPIQPDYWCRRFDITEP
                     SEDRWIHYMSHELVGLTDEELKEYYYKDDPELKAILDSIMKKKG"
     gene            28925..>30562
                     /locus_tag="PKW26_01515"
     CDS             28925..>30562
                     /locus_tag="PKW26_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92279.1"
                     /translation="MRLRRITVFSLFFFMLGGFYAFDNLSIQEDASLYPKADSEAVLS
                     PVSGTWANYQSLVLDVPKDAIALYSFTGDDPLEFGFAYDGPVRIEKEGEVDLRIAFVY
                     ADNTVLETQVSYRVVLPGLLSLGEEGAFLNQNQPFIRASADNPLKIPSDFLYSLDDDR
                     LPHARGRTLFLAENTYERYLPLLVAVDDQVYRWMLFLEKSNESSTEKKPSEARAFSTK
                     LSLSNPDFLFRLSDRPESPLVQSLFISVLEGEFFSKRISIDVYDGNEFIGSLEKMLSV
                     NRSLPKKPEFISSAKSFYSREAVKLKLSHNETFFYLVDEPEILHFDDKTVSYQIAEKK
                     EPTLAELRSGYKTKGLEIEFPTHSEAVLYRIYAIAEDSEGNLSDIASYECIVDANQYY
                     IDPRKKTEQSAKNAFYPADGSPDFPFLSLEDALKKLSNKNGIHLHLLGNDVIQNTLQI
                     NQDFSLIGHNDAELQLGSDVYFEVRDAKLSFESVFFTSDFQKNSKQSKEKISSLAFQK
                     SLFSLSDAQVLFENCDFSFFHPGNFVLAQARNSRLDFY"
     assembly_gap    30563..30662
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            30738..31970
                     /locus_tag="PKW26_01520"
     CDS             30738..31970
                     /locus_tag="PKW26_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92280.1"
                     /translation="MKRWLILFLFFFAYNSYAQSFFFDTLLELSSYWDSFIGAKEKRD
                     KSTESDLDAASYAARYTESFIALLESFLDHKLDVENDAQKKRAEKIDDSLAELKMQNE
                     DRGYAEALLYMELLFYTSYARDFFIEADQKLNQLIAEVFERKFTPTYIAENEYLFLSR
                     SLFYSLHARIEKHVSSHWLPDLEDTEVFFKASLRRLDSGLSAKEIRAKQYSALSSVES
                     LYSNYLAFQKSQDSKKRFLFSEEMFYSFLNKPSFLYYLFHMESYFPLRDAFLSSAEIF
                     LEIDQGIIEEIKKAEFDFLYDSSFSFFYRYFYKDLYAKIPDDKRKESIALVYKTNDLF
                     YQSFIFAAKYGFSIDAVLTEDNQLLYSLFLSDPFLLSLYDAETMGAFCDNLIFIQMLS
                     DIYINRINRLPSIQRLKG"
     gene            31973..33118
                     /locus_tag="PKW26_01525"
     CDS             31973..33118
                     /locus_tag="PKW26_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92281.1"
                     /translation="MAKKNPVFLFFIWLSFFILLFFAGFFIYQKELIPQFNFWLNSFF
                     YKESKPNAASLPEIEPIRIASGEIGEMEAFAYKIRDSQFLIDIEAFFREDFFDNTNSF
                     SMSYSEEQKTMKLTGMFQRLDADFDPKDESIRSWTSLQAAPLSAPSLFQDSLVFIDTD
                     LNLIVLDLKTGALKQSFPTPVYPEGLALAVQAPLSLSGIYQSDDLVPQYIIKAADGNV
                     YAFAFFDPLLYEDVKTKISLISDEKNKHPFWPSQEVEKSMMEATATWAQYAAVPDFIE
                     PKLLLSDGSLLRVNAEGISVIALMFKEEGQYQMGMSDASSLFISADALACFFSQTGEL
                     LSVSVDYESHKPQVQVLLEADTLYYIVLANKGELPLNDVYFSYKKIK"
     assembly_gap    33207..33306
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <33307..33532
                     /locus_tag="PKW26_01530"
     CDS             <33307..33532
                     /locus_tag="PKW26_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOQ92282.1"
                     /translation="KIDCLAIPKADANYPSVMAASILAKNERDRIMCSYAKLYPEYLY
                     EQHKGYPTKQHREICKTKGPSPIQRKTFRF"
     gene            complement(33545..33748)
                     /locus_tag="PKW26_01535"
     CDS             complement(33545..33748)
                     /locus_tag="PKW26_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOQ92283.1"
                     /translation="MSEKRLYKSRNKMLCGVCGGVAEYFSVDPTLIRLAVVFLSVAGV
                     GSGLLAYIIAAIIMPEKPGNMDE"
     gene            complement(33768..34028)
                     /locus_tag="PKW26_01540"
     CDS             complement(33768..34028)
                     /locus_tag="PKW26_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92284.1"
                     /translation="MKKISKTLLILALVLFILGSFITCSALTKIAGDYTIAGIFNDEF
                     EEIFPLEIEIKTPKKYKFIDINSKLFNIKIKMNDRDENTDEW"
     gene            complement(34025..34687)
                     /locus_tag="PKW26_01545"
     CDS             complement(34025..34687)
                     /locus_tag="PKW26_01545"
                     /inference="COORDINATES: protein motif:HMM:NF019618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HOQ92285.1"
                     /translation="MEKEEYLRLLEIELAALPLEEKENVMEYYRSYFEEAGEENHNRV
                     IKELGKPEELAAHILKNSSCLPSVLGKKNKSDQQEKTEERKNKTMPKDQKNENRGNKI
                     LLVLLILVFSFPLWFPLICSIFGILFAFVVVTLSIGFIGIIIGLALMIASIVLGTFAL
                     FSMILFTPSFFLFLGLSLLLMAFGIVGLVLGIWFCKKMLPPLVRFIVSLFSIPFRNRS
                     RV"
     gene            complement(34696..35016)
                     /locus_tag="PKW26_01550"
     CDS             complement(34696..35016)
                     /locus_tag="PKW26_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOQ92286.1"
                     /translation="MQFSSGTALLDSIVLSLLAKEDSYGYKITQDVQEILFISESSLY
                     PVLRRLQKENCLETYDGEFSGRNRRYYRITSNGMIKLDNYRREWIKYRKKIETVLLGL
                     NTVD"
     gene            complement(35173..36846)
                     /locus_tag="PKW26_01555"
     CDS             complement(35173..36846)
                     /locus_tag="PKW26_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOQ92287.1"
                     /translation="MNAKELLSKMSLEEKAGQLIMSSFVDRYDIPEDMQKLLETGAVG
                     SILYFSGCNVVDSLQLRDLTEKVQAASLKSPHKIPQFIAIDQEGGQLAPITKKISIGP
                     GNMALGAIRENAEKHAYEMGKVTGKELKAIGVDVCFAPVVDLCFEEGLPVKDNRYFCS
                     NPQQASKLGAAFTKGLQEEGVAACAKHFPGQRNVDIDSHFELDEVPHDFERLMSHEWV
                     PFKGAIDAGVSMMMTLHASFPKLDETKTPATLSKPILQDYLRGKLHFEGLIVTDDIQM
                     EPIKNVYGIGGAMVKAINAGVNLIIVSGGVYDAHIQIADAVRKGELSEETLNAACLRV
                     LEYKAKYVNTNLLSEKKAAKIVASKAHTKAIQDAADASITLLRNKSNLLPLRKIDPKA
                     RIAIVRPSFGRLMMSDNTNFYLHNMKEIFSQYFDNVSEYVFGLNPSDPEILGASDWAF
                     MADYIVLCTYNAYQYPKQLELIQKCFSYAKNEKTILTVLRSPQDLVVIDDEIDTVLAT
                     YGVAECSIHALARTIAGINTPSGALPVGVGKDQGYKNNSLKAGSGQNGF"
     gene            37015..38544
                     /locus_tag="PKW26_01560"
     CDS             37015..38544
                     /locus_tag="PKW26_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOQ92288.1"
                     /translation="MITHTSFIPAVQNLSTETGWVETRNALIHFEPMFERLSLLAKEQ
                     LSKAGLLSSVQSPNQAELLVQGSVLKIYLQEDKNIAHDEGYELSISGKTAKLKAKTEA
                     GMFYALQTLRQLVYSSEAKIPYITVKDYPAFEWRGFMLDTSRHYYSADFIKKMIDLAS
                     FHKLNRFHWHLTDDQGWRLPVKAYEKLTEVGAWRDDPRYSWGAIKGGFYTEEEIKSVV
                     AYARERHVIVVPEIETPGHASAILASYPELGCTGGPYAVETRYGIFKEVLCAGNDAVF
                     SMLEKVFDTVCELFPGPYVHIGGDECPHERWEECPKCQKRIKDEGLENASELQSWITV
                     KVSKMLEERGKIPIGWDEVLDGTEKLGLPKNVVVQSWRGVEGGIKASSLGHKVIMSPL
                     TDGCYLDYKHFDTEEEPGMHKVTTVADSYNYSPIKPEMTKEQRENVLGGQGNIWTEVM
                     YASKIAEYMAFPRLSAISESLWLEEEKKDFLSFAKRLIKHKQRLDRLNVLYYKGQLSA
                     Q"
BASE COUNT        11784 a         6847 c         8012 g        11751 t
ORIGIN      
        1 tttctaacac ggatgttaga aaaaatgtaa gatggttctc ttcccctaag aaacgtaaga
       61 atcaaaaata taaacgaaag attgcgaatg ataaaagaaa aaatggatga gttacctgaa
      121 atagaagact taataaaata aaacgcataa aaaccatagc ctgcaaaata aacctgcagg
      181 ctatgccact cgcttttgcg aacagctaat tgtgtgtaca ggattaacat gataaaacag
      241 gatacacagg aagtaaaata aaaacttcta gattagcaag cgttgcaagg agagatgggg
      301 ttttagggga agagaaactt cgcatcgcaa aagatggttc tcttccccta agaaacgtaa
      361 gaatcccgtc tatcctgtat gctcgaacag ctttgcgttc ctgtcgcttt taagtggata
      421 acatagtata ataggcatat ggatatttta ctcgaatact gtgcgacaga aattatcgta
      481 aaaaaatctc ggtttatagc ggaacttttt cctatagaaa cacaggctga agcacgagat
      541 gttttaaaaa atcaaaaaca aaaatacgcc gatgctgccc acgtagtcca tgcctttgtt
      601 tgcgggcttg aaggacaaat atctggcatg agcgatgatg gggagccttc tggtaccgct
      661 ggtcgtcctg cctacgatgt cttaaagggg cgaaatctaa ccaaccttat cttaaccatt
      721 acgcgctatt tcggaggcac tcttttagga accggaggac tcgtaaaagc ctatggagat
      781 gcagcccgag aagttctcga caaggcaaag acagaatgct atattcccaa aataagcttt
      841 tgttttaagg ctccctacga agtctacgaa ggcataaaaa gaagcatcgc tcactttccc
      901 ctcacaaatg caaaggaaga attcttagaa agggtaggct tccaagcaga aatccccctt
      961 gagcacaaag aaaatctttc tgcaattata aaagatgtat caaaggcaat ggtcacagta
     1021 gaatttctat aaaaaagccg cctttcggca gccttttttt tatttaacac gtgtgtatat
     1081 taatccgtcg tgtttcatga tgatagtatc ttcatctaag gttccaatac caacaccatc
     1141 tgcactaata aaacggatgg tgttacctct cagctcaatt ctttctggaa catcgccacc
     1201 atcaacagcg ttacccatct ctgtggcaaa ccaagcttga ccgtttttaa tttcaagcct
     1261 ttgaaatgcg tcaaaatcca tgattttaat tggtgctttc caaactgttc cttcaagaaa
     1321 actatggtcc ctaatttcat ccagagcttg ggaagtctct tgctgattat cggcattttc
     1381 tagttgcttg gttgaagcac aagcagtaaa tgcaattgca ataatcataa taaacgcgaa
     1441 aaataagaaa tgttttttca tatttcctcc tgattgcata atcataattc tacaatttgt
     1501 ctttgtcaag tttttatttt tttaaaattc agcggacttt ggtgttcgag gaaaggggat
     1561 tacgtctcgt atatttttta agccagtgat gtataataaa agccgttcaa agcccaaacc
     1621 aaaacctgag tgtggtacag agccatattt tcgtaaatca agataccacc agtagtcttt
     1681 tggatcgagc ttcattgcct taattttttc aaggagtata tcgtagtcgg cttcacgctc
     1741 cgagccaccg attatttcgc cgagcccggg cacgaggacg tccattgcac gaactgtttt
     1801 tccatcgtcg ttcagtttca tataaaatgc ttttatatct tttggataat cagttacgat
     1861 aactggtttt ttatacacgg tttcggtgag atagcgctcg tgttcacttt gtaaatcgca
     1921 accccaataa ggtttgtatt caaacttgtc tgcatttttt tcaagtacag cgattgcttc
     1981 tgtatagcta aggcgaataa aatcagatga tacaacatgg cgcaatgttt caatgatgcc
     2041 tttttgtaca cgatcttcaa aaaattgcat atcttcgcta catttttcga gtgcccagct
     2101 aagcagatat ttaataaaat cctcagcaag ctgcatattg tcttcgtttt tgaaaaaagc
     2161 catttcaggt tctatcatcc aaaactctga aaggtggcgc gttgtgttgg agttttcagc
     2221 tcgaaaggtt ggaccaaagg tgtagactcg tgaaagagcg gttgcatagc attcagcttc
     2281 aagctgtcct gaaacggtaa ggcttgcatg tttcccaaaa aagtctttgt tatagtcgac
     2341 aagcatgtct gcctcatctt cactttttcc acccttacca gcttttatgc ctgttcgtgc
     2401 aagctcttct aagttgagcg ttgttacttg aaacatctcg cctgcacctt ctgcatcact
     2461 tgcggttatg atcggtgtgt gaacatagtg aaaaccgcgc tcttgaaaaa aagtgtgaat
     2521 tgcataggac atttggcttc tcatacgggc aacagcgcca aagagatttg tgcgtggacg
     2581 aaggtgcgca atttcgcgca aaaactccat gctatgccct tttttttgga gggggtagct
     2641 ttctgttgga gcttcgccaa gtactcgaat agagctcagc gcaagttcta ccgcttgtcc
     2701 ggaagctgga gaaggtacta aggttccgac tgcttctatg gaagcacctg tcgtaacttt
     2761 ttcaagtgct ttttctatag catctgtatc tacgtttgta ttttctcggt caaaggtgac
     2821 ttgcatagag gtaaagcaag agccatcatt tacttgaata aaacagaggt tttttgaatc
     2881 gcgctttgta cgcacccaac cttgtacgca aatttctcgt ttatctggag atgagactaa
     2941 aatatcttta ataagttctg ttttcatagt ataagtagta gcattttttt taaaaacagt
     3001 caaactgtaa aattattttt agcctttttc ctcttgacac aattttaata tttagctata
     3061 gtcaataata caagggggcg tagcgaagct ggttatcgcg ctggcctgtc acgccggaga
     3121 ccgcgggttc gagtcccgtc gctcccgact aaagctgtgt atcatttgat gcacagcttt
     3181 ttttttcctt gatagcctgt ttttcacaag acttctcttt tgcgttaaga tatatcactt
     3241 ttttttctct tctcgggatt tttttttata cctatgacat tttttcgttt tttgtcttga
     3301 taaaatacgc attaggcggt acactgaaaa aacagtattt tttaggatag gaatgataaa
     3361 aacatggcaa atacagaatt attagatcta acgataagtg aaatgctccg tgagcaaaca
     3421 aaaaagaatc caaaccaaga ttttatggtt tattccgacc gtgatttgcg ttttacttat
     3481 gcagccttcg atgcacgagt tgatgcgctt gcttgtgcgc ttttggcgat tggtttaaaa
     3541 aaaggcgatc acctcggtat ttgggcgaca aatgtaccag attggcttag ttatatgttt
     3601 gctgcgacgc gtatcggtgt agtaaccgtt actattaaca cgagttataa attgcatgag
     3661 cttgagtatg ttattaagca gtccgatata cgaatgctct gtatctccga tggaatgaag
     3721 gattcacaat atgtgagtat gataaaggag ctcattcccg agcttgacac atgtcaaaga
     3781 ggcgcacttg taagtgaaaa atttccagaa ttaaagcagg ttgtttttat tggaccagaa
     3841 aaatatcgtg ggctttattc aactcatgaa ctgcttttac ttgggcaaca tatcgatatc
     3901 gctagagtac atgaagttga acaaggtctc aattgccatg aggtaatcaa tatgcaatat
     3961 acctcgggta caacgggttt tccaaagggt gttatgctct ctcatcacaa tattttgaac
     4021 aatgggtatt ttattgggca atgtatgaag tatagcgaaa aggaaaaggt ttgcttgccc
     4081 gttccgcttt ttcattgttt tggcattgta ctcggtgtta tggcggttct ttctcatggg
     4141 gctacccatg ttatgataga aagctttgac cccctccttg ttcttgcttc tgtccataaa
     4201 gaaaaatgca cctccctgta cggtgttccc acaatgttta ttgcagaact caatcatcct
     4261 atgttcaata tgtttgatat gtcctcactt cgaacgggca ttatggcagg ctcactttgt
     4321 cccatcgatg tcatgaaggc tgttatgtca aagatgaatc ttacagagat tactagtgtt
     4381 tatggtttaa cagaaacctc tcccggtatg acgcaaagta gaacagatga cccgatggaa
     4441 attcgtgcaa caacggttgg aaaagaattg ccccatattg aagttaaggt gctcgatcca
     4501 gaaaccgggg aggaatgtcc tccaggagta caaggcgaaa tgtgttgccg tggctacaac
     4561 gtgatgaagg gctactacaa aaatccagag gcgacagcag aaattatcga tgcaaatggt
     4621 tttttacatt cgggcgattt gggtataaaa gatgaggctg gttattttaa aataacaggg
     4681 cgcatcaaag atatgattat ccgtggtggg gaaaatatct atccaaaaga aatagaagaa
     4741 tttttacgac aaatgagcga aataaaagat attcaggttg caggagtgaa ggataaacgt
     4801 cttggagaaa ttacaggagc ctttattatc ttgcatgagg gaaaaacgct tacagaagaa
     4861 gacgttatag aatactgccg tgggaaaatt gcaaaataca agtttccaca actggtttta
     4921 tttgtcgatt catttccgat gacagcgagc gggaaaatac aaaaattcaa gctttctgag
     4981 ataggggaag cacatagaaa atcgcttgga attgagtaaa cttctccttg acttcttttg
     5041 tgtcatttag tttactagta gagagagtat agggggtaga aatgaaaggt aatggattat
     5101 ccatggaaat agctcagagg ctgaagggct tacgagatat tatggagctt tcaacagagg
     5161 acgtcgcatc ggtttgcggt atttctcaag aggaatacga aaaatacgag tctggtacag
     5221 tcgatattcc ggtaagtatt ttacatagca ttgcaacggc gtataagttt gaaattacta
     5281 ccttgatttc tggagaagag ccccgcatgc attcgtattt tctaaccaaa aaagataagg
     5341 gactcggtgt tgacagaagg gcaaactaca aatacgaatc ccttgcgcat ggttttatca
     5401 atcgaaaggc tgacccctac attgttacgc ttacgccgga gcaaacaaga gaaatgcact
     5461 tcaattctca ccctggccaa gagttcaatt atatgctcga aggttcaatg aagattgtta
     5521 tcgacggtaa agaacttatc ttagatgcag gcgattcaat ttattttgat gcaaataaaa
     5581 agcatgcgat gcaagcttta aacgaaaaaa acgcaaaatt tttagcaatt attttttaaa
     5641 gaaggcagat aatgttaaca aagtatgtac cgaagataga tttttcttca tcacaagatt
     5701 ttagagataa ttatacagtc atagttcccg agcgatttaa cttttcctac gatgtactcg
     5761 ataggcttgc aatggaaact cccgatgagc gcgcccttgt ttggtgcaat gatgagggag
     5821 aagagaaaat ctttagctat agtgaaataa aaacctattc tgaaaaggct gcctcttttt
     5881 ttcaagcaca gggaattcgc cgtggcgaaa aagtaatgct tattttaaaa cggcgttatg
     5941 agttttggtt tgcgctttta ggcttgcatc gaattggtgc cattgctgtg cctgcaaccc
     6001 acatgctcac caaggacgac cttgtgtatc gaaataatgc ggcaagtatc aaaatgattg
     6061 ttgctgccta cgacgaaaaa gtaatggagc aaatcgaagc ttccttagaa gagtccaaga
     6121 gcgtagagct tttagtttct gtttcgggca taagcggttt atacgaaaat tctttttaca
     6181 ataaaaaacg cgacggctgg atagatttta atagcggctt agaagaagca aaggatttta
     6241 tagcaccaaa acgagaagag gtaaacgaca acgaggatat aatgctcttg tattttacct
     6301 caggcacgac aaatcatcca aagatggttg cccacgatta tacctatcca ctcggccata
     6361 tcgtaacagc gaagtactgg caatgcgttc gtaagggagg cttgcatctg actgttgccg
     6421 acacaggctg ggcaaagtca gtctggggaa agctctacgg gcagtggctt tgcgaatgtg
     6481 cggtttttgt ctacgattac agtgcaaagt ttaaaccagt tgatttattg gcacagattg
     6541 aaaaatataa gattacatct ttttgtgcgc ctccaactat ttatcgcttt cttattcaag
     6601 cagatatgtc aaagtataat ctttcaagct tggaagaatg ctgtactgct ggggaaccct
     6661 tgagcgagga agtgtataac cgcttcaagg cacaaacagg gcatggactt ttggaaggct
     6721 ttggacaaac agagacaacg ctttccctct taaattttaa aggcgagtct gtaaagcctg
     6781 gctccatagg aaaacccgcc cccttttaca atataaaact tcttaaagat gatttaagcg
     6841 aatgtgttga cggtgaaata ggcgaaattt gcttcccact cgataagggc tcatttccgg
     6901 ggcttttcaa agaatattat cgcgatgcag aaaaaacaag ggaagtaatg cacgacggct
     6961 attattttac tggggacacc gcctggcgag acgaagacgg tttttactgg tttgttggtc
     7021 gctttgatga cattataaaa tgttcgggtt accgcattgg gacctttgag gtagaaagta
     7081 ttttaatgca acatcccgcc attgtcgaat gcgcggttac cggttttcca gatgaagtga
     7141 gagggcaggt tgtaaaggca actattgtac tctcagaaaa atacaaacct ggtagcgatg
     7201 tccttgcaaa agaaatacaa gactttgtaa aaaaagcaac agcaccttac aaatatccac
     7261 gcatcgtaga gtttgttgaa gagctcccaa aaacaatttc tggaaagatt aaacggcgcg
     7321 atattcgagc aaaggattaa gcatggcgaa ggtgcttcga tcgaaggtgc tcggtttttg
     7381 catgggtgta aaacgagctg tcgatatggc agaggaagtc ttgaacgaaa atgaggggaa
     7441 agaagttttt atgctcggtc ccctcataca taatgagcaa gccttgcaag ccttgtatga
     7501 taagggcttg cacttactga gcgaagaata tatagaagaa agatgcaatg aaaaaagcat
     7561 tgttattatt cgcgctcatg gaacatctcc ttcaatcctc actcgcatag aaaaaacagg
     7621 tgctcgcata gtcgatacaa gctgtcctcg agttaaggca agtcaaaaac gcgcagcaag
     7681 ctattctcaa gatggctttc ttgtttttat tgcaggcgac ctcggtcatg gtgaagtcct
     7741 atctatttcg tcatatgcag gagataaggc aattgttttt aaagatgcgg aagaagcaaa
     7801 gacttttttt aaaacgtctg agctatttca aaaaaatcca agagcctttg acaaagcgat
     7861 tctcatttca caaacaacaa tacgcaaaga agaatacgat gcaattatcg cggttttgca
     7921 aaaaaaactt ccacagattg aagtattcaa tacaatctgc tcggcaacaa aggagcgcca
     7981 acttttttta cgagagctgg cacacgaagt tgacgggatt ttggttattg ggggcaggca
     8041 ctcggcaaat acacaaaggc ttttgtcctt ggcccaagaa ctctcacccc acgcagccct
     8101 cgtcgaagct gctcacgaaa taccacctga gttttttcgc ttagaaaaag tcggtttaac
     8161 agccggagca tccaccgcaa ataaaagcat cgatgaggtt gagcaaaaac ttttttcaat
     8221 gaatgaagaa taataaaaat tctattcttg ctttttttat cgattattgc tagtatacag
     8281 gttaaaaagt acaagctttt tttataaacc attattttac gattgtggaa tgaggaggtc
     8341 tttatgatta agacaataaa agatattgat ttatcgggca aacgtgttat tatgcgcgtc
     8401 gattttaatg tgccaatgaa ggatggagta gtgcaagatg atacaagaat tcaagctgca
     8461 cttccaagta ttcaatatat cctagagcaa aatgtgcgct ctcttgtttt gatgagccat
     8521 ctcggagatc ccaaaaaaga tagcaaaaaa gcacaagaaa aggctgaaaa agacggaaaa
     8581 ccctttgata aagaagcata tattgcagga aagcaccgca tgaagcctgt tgccgactac
     8641 ctttcaaagc ttattggtaa agaagttgtt tttgctccat catgtcttgg acaaaaagca
     8701 gcagtcgatg cccttgcaga aggggatgtg atgatgcttg aaaatacccg ctttgaaagc
     8761 gccgaaacag caaaggacct tgctgaacaa gaagccatgg caaaagaact tgcaagctat
     8821 ggcgatgtct atgtaaacga tgcctttggt accgcacata gagcacatgc ttcaaccgca
     8881 actattgcac aatttataga tgtaaaagtt ggcggcttcc tcatggaaaa agaagtacaa
     8941 tatttagaac caatggtttc aaatcctcca aaacccatga ttgcaattat cggtggtgca
     9001 aaggtttctt caaaaatcgc tgttctcgaa agcttgctta aaaatgcttc agccctcatc
     9061 atcggtggtg gcatggccta tacattctta aaggcacaag gctatacagt tggtaaatct
     9121 ttagttgaag atgattttat cgataccgca aaatctcttc ttgctgctgc aaaggaaaaa
     9181 aatgtttcta ttattcttcc cgaagatcat ctttgtgcag aaacatttag tcctgaggca
     9241 aaggctatcg ctgttgatgg gcaagacatt gcagataatc ttatgggaat ggatgtcggt
     9301 cccaaaacaa tagcaaaata caccgatgcg attcttaacg caaagtccat cgtatggaat
     9361 ggtcctgtag gtgtttttga atttgatgcc tttgcaaagg gaacaaatac ggttgctgac
     9421 ttagttgctc aagcaacagc aaagggagca atctctgtag ttggtggtgg agattctgtt
     9481 gccgcagtca ataaagcaaa acttgcagat aagatgagcc atgtatcgac gggtggtggt
     9541 gcttccttgg aattcctcga aggaaaaaca ctcccaggaa tagcagtttt agctacaaaa
     9601 taaaaatata catttagaga aaggaaaaaa aacatgagac aaaaatacat tgccggtaac
     9661 tggaagatga acaaaacaaa gggagaagca cttgaacttg caaaaggttt agttgcagct
     9721 cttaaagatg gaaaaaacaa atacttaatt gcaccttcat ttacaaatct tgatgctgta
     9781 gcacaagttg taaaaggttc aaatattcgc cttggcgcac aaaacatgtc taccgatgag
     9841 tcaggcgcac atacgggtga agtttctgta ttgatgctga aggaccttgg cgtagaaacg
     9901 gttattcttg ggcattcaga aagaagaact atttacaaag aagatgacgc tatgatcaat
     9961 aaaaaagtaa aacttgcctt gcagcatggg ctcgaagtta tcctttgcat tggagaactt
    10021 ctagaagaaa gagaagcagg aaaagctgaa gctgtttgta aagcacaaac agaaaaaggc
    10081 ttggcaggtc tttcaaaaga agatatgaaa aacgtcgtta tagcctatga gccagtctgg
    10141 gcgattggaa caggaaagac ggcgacgcca gaagatgcac aagctattca tgcctatgta
    10201 cgagacgtac tcgcctcact ttttgacaaa gaagtggcag atgcaacgat tattcagtac
    10261 ggtggttcca tgaatgcagg aaatgcaaaa gatttactcg ctcaaaagga tattgacggc
    10321 ggtttaatcg gcggtgcagc tctaaaagtc gacacctttg aagcaatttg taaagcctac
    10381 tagaaagggc catcgtttta acgagatttt ctaaaatcta agaaaggtac aaggctattt
    10441 tcattatacc cttgtacttt ttttttattt acggtaaaat gaaatatcaa aatatactat
    10501 tttaacaatt tttaactatg gagtttttat atgggtgttt taggaattat tttgctcatt
    10561 gcatttatta ttgtctgtct tttacttgtg gcgattgtac ttttacaaaa tgaagaaggc
    10621 gatggcttag gtgggctttt aggcggagca agtaatgttg cttttgggtc aagagctgga
    10681 aatgtgctca cacgaacaac ctatgtctta gttacacttt tttttgcttt gagtattgga
    10741 cttgctttat tgaacaaagc tccatcagtt gatgcaaagg gtcttgaaga tgctgctcgt
    10801 gcacaagatg cttcaggaac agaaagcgaa tattggttag aagaaacaag cagcgacaat
    10861 cccgcaccga gccttttaga atctgcagac acagatttct agttacggta cgcagatgtt
    10921 tttaggaaag atttttaatc ctaaggcaat aaagctcaat ttagaaagcg attttaaaga
    10981 tgaggttttt gaagaactta tagaagtctt agtttcttca gaaccctcat tgaatagaaa
    11041 tgaaatactt ttcgctttgc aagaaagaga gcgtttgatg agtactggta taaaaaaagg
    11101 tgttgctcta ccacattgta aaataaaggg tattcaggga gtgcgaggcg caatcggtgt
    11161 ttcccaaaag ggtatagagt acaatgcact cgatttaaat cctgttcacc tcgtttttat
    11221 gattgttgcc tcaagtgagg atagccttgg ttatttacaa gtcttaaagc aattagcact
    11281 catactcaat gaacgtgcat ttttacaagg gatattggaa caaaatacgg ctgttgatgt
    11341 tcacgaactt ataaaatcat acgaaaaaaa aatggctgaa aagaagataa tttaggagga
    11401 tcagaatggc tggtgccgat gttataagtc atcttattga aattgaaaaa agggcggctg
    11461 aatttatgca tcaagcgcac gtagaagctg acgcacgttt aacaaaggca cgaaccgagg
    11521 ttgattcgca atttaaagaa gcttatgaaa agcttatgaa agagcttgaa gctgactata
    11581 ctaaaaaaag tattgccctt aaagaacaat acgaaaaaga ttttaacgac tttgttcgtg
    11641 aaattactga aactccacaa gatacagaag caattcgcac cttgttttta gaattactgg
    11701 ataaaaggtt ataaggtaga tatggataag tcggcggcac atgcctttgt ttttgcaaag
    11761 gcttctggga tgctttctaa atcttttgtc gggaatcgct ttttaagtat atttcaagaa
    11821 aaaaaactac aagacttgtg gaaacttctt tttgaagatg aagtacctct tctgcccgaa
    11881 tatcttttgg cacaaacaat agaaaaaaaa gctgaggaag tctttgtttc cgattttaca
    11941 cagcttttat cctattatgc aaagcccgac cctgtcctcg tccagctttt acaagtatat
    12001 gaatacaata atctcaagct tatttcatcc gctttaatga acaataaaac gcagatgccc
    12061 gagattattg acttgcagtc tttttccatc cttgattata gtaagtggcc agatataaaa
    12121 aaaatgacag aaaatactgt ttttgcttgg tatgatacta ttcccaaggt aaaagagctt
    12181 aaaacgagag accatgcact ggatgtacaa tatattcgaa gtttatggac ttctgtgcag
    12241 cgtctacctg ctgcagagcg tgcaccggta caagctttta tcaaagaaga tatcgttata
    12301 caaaatattc tttgggcgct tcgcttaaag tattattatg gctatgatgc cgaagctatt
    12361 agaccttttt tggcgggctt aaaagataat cctgatgaaa aagatatttt agcaggacct
    12421 gctgtacaaa ttttagataa ggaaatagat tcttttgctg attggaaaga ttgggaatat
    12481 aggcgttttc tcaatcgcca tgaaccagat gaaatatggt ctatcgatcc aaagtgggtc
    12541 gcacaagcgg cggaaggcta tttgacaaaa aaagccttga agaattttcg aaaatatccg
    12601 tttacggcga tggttttagt gtgttggttt aaagtaaagc aagcagaagt ttcctatgtg
    12661 cggacagcgg tgcaagcttt gcgtttaaat gtgagtgaag aagaactttc tgagtatatg
    12721 acttagccag aggaggacgt atggcacgaa cagtatctat gcagctcata gaattgatga
    12781 ttcacaaacg agacattagt tttgtattag aatacttggg gaaaaaaggt gattttcagt
    12841 ttcaatcaca tattgatgat gaaaaagctg cggaaacaag tgaagaatcg agagcttttg
    12901 actccttgca agatatccgc tcttatttac atatagaaga tttagaaacc tttgttgagg
    12961 gtgcaagtct tcccacagaa ggcgaagcag cacgcgtaaa ggagctgagt gcttcagttc
    13021 gtgatttacg agaaagggaa ataggggcaa aagaagcgaa acaaagattg gaagaaacct
    13081 atcaagaggc gctttctttt tctaacttaa aattacccta ttctgagctt gaataccttt
    13141 cttttttatc aatacgtatt ggaaaaatag atcctgccgc tatagattcg ctttcctttt
    13201 cccttggaga tagagggctc attattccac tcggtgaaga taaaacacgt attctcgcag
    13261 ccgcctctaa aaaaggtcgc tttgctctcg atgctgaatt aaaacgtgct ggctttgtgc
    13321 ctctggaaat tccagaaaac tttcaaggaa ttcctgatga tgtcatagtt gggatgaagg
    13381 aaaaattgga tgttgcggca aaagaacttg aagcaatcga aacagaaaaa cgtaactttg
    13441 ctcaactaca tactgatgaa atctatacat tgttaagctc ctattctttg gctatgcaag
    13501 ttgcttctat gcaatctcgt ttagaggcaa cacaatatgt ctatcgtata acaggttgga
    13561 tacccaaaag tcatatgcgt ggctttacga gtgatttaga taatctttcc gaggggcgaa
    13621 ttgcaatacg gcaatatcaa cctgaagagg ttccgtctgt tcgtgctggg caagaaaagg
    13681 tgccggtaaa gctacaacat ggtaaatttg tttcagcctt tgagagaatg gtttttagct
    13741 atggcgctcc tttgtacgga actatagatc caactccgat cgtcgccttt ttctttactc
    13801 ttttgtttgg aattatgttc ggcgatgcgg gccagggact tgtgtttctt ttaattggta
    13861 tcctaatgac agtaggggta ataaaaaaat tccccgtaga cccaaagttt ggggctattt
    13921 ttatagcgat aggttgtagt agtactatta tggggctact tacaggagaa ttttttgcaa
    13981 atgagcatgt acttattccc tttgggcgtt ttgttaccgg gctctttggt gaaagccaag
    14041 acagagtctt acatctcatg cccgcagctg attccctcga taagctcttt tatttctttt
    14101 tgtttaccat agcgaatggc tttattataa attccatagg tttaataata aatattgtca
    14161 ataatttttt actcaaaaaa cctgaaaaag ctgtttttgg taaaacaggt atgtttggct
    14221 tgctttttta ctggtacatt gcctttgtta ttattcggat ttttgctttt aagtcgactt
    14281 ttatgctcta cgattggata gctataggaa tcactctttt tggtgtcttt tttgctcatc
    14341 ctcttgaaag gctttttaca aagcaaaaac ccattttccc attaggaatt ggaacagcga
    14401 taatcgaagg gcttgttgaa atactcgaag tactttccag ttatctttct aactcggtga
    14461 gttttttacg tgtgggcgcc tttgcacttg ctcacgcagt tttgggctat attatatttt
    14521 ctatgacagc cttggtgcaa tcaagcggaa cgctatttgg aatagttggc ggtgttttgg
    14581 tttcgattgt tggaaacctc attgttattg ttttggaagg tatgattgtc gccattcaag
    14641 ttgttcgatt acaatactat gaattctttt caaagttctt tagtgaagcg ggaagagaat
    14701 ttgaaccctt tgtctttgtc tacaaaggtg caaaaggata gataaggcga aagcctttat
    14761 ataaatgttt gcgttgcaaa cctgctaata ttatcgtact tctgtatcta aggtgaagta
    14821 caaagatgga gtaaaaatga aaaagaaaat tgcagtttta atgttctgta tgctcctgct
    14881 ttcaagcgct atgctttttg ctgaagcaga aacagttcaa aacgtaacag atttttcttg
    14941 ggtgaaatac ctcgccgcag cccttgcagt tggttttgct tgtgtcgctg gtggcatggc
    15001 tgttgcgaaa atcggtgcag ctgctatggg tgctatgagt gaaaatgcag aactttctgg
    15061 taaagcgctt cccttcgttg ggcttgcaga aggtatttgt ttgtggggct tccttgttgg
    15121 acttctcatt cttattctgt agttaaaacg gaaataccga gtgcaatatt tttttattgg
    15181 cgaaagagaa cttgttttag ctttttctct tgttggtgtt gctggtgaaa ctgtctcaaa
    15241 tcgaaccgag atgctcgaag tctttgaacg acttacgggc aaaggttcga gagacttgca
    15301 tgcacctgtt ttgagcgaaa ggccgaaaat tcttatttta acagaagagg ctgcctcaat
    15361 gattgaagac gaaattcgtc agtggcaaat gaagggctca tttcccctca ttgtagaaat
    15421 accgggtctc cacggacata tcgaagggaa aaaatccctt accgatgcaa ttcgtgaagc
    15481 gattggtata catatttagg atggtgataa atggaagaac tgagatctac cgaattactg
    15541 gataaagaaa tactcgaaga tgccagagtg aaggctgagc atattttaca gtctgctgat
    15601 aaagaagcag aaaaaattct ttcttctgta tcacaaaaaa ttgatgcttt aagacaagaa
    15661 aaagaacttg tttttcaaaa taaattagcg tatttcaaaa aagacctcga tgcaaaaatt
    15721 cctttaaaaa aacaacgttt taaggtttct ttccaaacag aaagtgtccg aaagcaaatg
    15781 tacacacata ttatgtctct ccctttggaa gaaaaaaaca aggtctatga atctatgctt
    15841 cgttcatata gcgatgttat tgctgaaccg tcttttatcg taaaggaatg ctattatgac
    15901 aaggatgcac ttgtgagcat cgttacaaat gtgtttggaa aaaaaagtat agatatagaa
    15961 agtttaaaag tctgtgaaac aggcatatta cataaaaatt ccgtagaagg atttaaaccc
    16021 gaaggctttg ttttagaagc gaaagatggc tccattgtct gtcgggttat gcttgaagag
    16081 cttatagaag gcttaatcga tacctattcc tttgaattag tcgatgcctt gttttcaggg
    16141 aggcttccag aatgattgaa ggtaaaatta cgagaatatc tggccccatt gtatatgcga
    16201 gtggtttaga tagttgtggc ttatatgatg ttgtggatgt tggggaagca aaacttattg
    16261 gggaagtaat acgccaaaat aagggtcttg caaccgttca agtatacgaa gatacaactg
    16321 gtatgcgtgt tggcgaaaaa gttgttagtc aacaaagagc gctttctgta cacttaggtc
    16381 caggacttat tggaactatt tatgatggga ttcaacgacc tttaaaagaa atgtatgaaa
    16441 aaagcggcgc ttttttactt ccaggagagc gccttgaggc ccttgatacg gaaaagaaat
    16501 ggacatttaa cccaaacgat gacttaaaaa cagagataga tggcagtgga aaaacactgt
    16561 ataaaataga agcgggcatg agcattggtt ttgtgcaaga aacgtcttcc gtaaaacata
    16621 gtattatggt tccgcccttt attcgaggaa aacgccttgt ttccttttct ccacaagggg
    16681 actatagcat aaacgagaca atagcaaaaa ccgaactagg ggaggaaatc aagctcgccc
    16741 attattggcc agtgcgaaaa ccccgaccct ttgcagaaaa actaatggtt tcagagccat
    16801 tgattacagg gcaaagaatt atagatgtct tttttccgct ttcgaaaggc ggaactgcag
    16861 caattcccgg cggctttggt acgggaaaaa ctatgacaca gcacgctatc gcaaagtggt
    16921 gtgatgcaga cattattgtc tatataggtt gtggtgagcg gggcaatgag atgaccgatg
    16981 tgcttaccga gtttccctcc ctcattgacc cgcgaaccgg gcgatcactt atggagcgga
    17041 caattctcat tgcaaacacc tcgaatatgc ctgttgctgc tcgtgaagtt tccttgtact
    17101 cgggtataac cctggcagaa tactaccgcg acatgggact cgctgttgcg attatggctg
    17161 actcaacgag ccgttgggca gaggcacttc gtgaactttc agggcgtatg gaagaaatgc
    17221 ctgctgaaga aggctttccg gcatatcttc ccacgcgtct tgcagagttt tatgaaagag
    17281 caggtcgtat tattagtctt agtaaaaaag aaggctctgt ttctattatc ggtgcagttt
    17341 ctcctcctgg aggggacttt tcagagccgg taacacaaca tacaaaacgc tttatccgat
    17401 gcttttgggc actcgatcgc gacttggcaa atgcacggca ttatcctgca atcggatgga
    17461 ttgaatccta ctctgaatac gcagaggaag tgcgctcctg gtgggataac cttgacccac
    17521 gttgggcaga gattcgtgga aaagctcttg atttactcaa gcgagaagag cgattgcaac
    17581 agattgtgcg cctcatcggc cccgatgcgc ttcccgattc tgagcgttta gtacttattg
    17641 tttcagaaat gataaaaaat ggctttttgc agcaaaacgc ttttgatgac attgacgttt
    17701 tttccgttcc tgaaaagcaa atcctcatta tcgatttaat gatgcagttt tacgagcgaa
    17761 gtgccgccct cataaaaaat ggctcgccct tgctaaaaat aagcgctctt tcgatacgag
    17821 aagatattat tcgtataaaa acatctgtgc caaatgatgc tcttgatgaa ataacgaagg
    17881 tgcgaatgaa gatggatgaa gaattttcga caattgagca tatttaccgt aaggcggtga
    17941 tttaatgaaa ggtgtagaat ataagggtct aaaaacagtt gacggaccaa ttgttattgt
    18001 aaagcgcagc gaaaatagct tttttggtga aatagtttat gtgcgagacc gacttggtga
    18061 aaaaaagacg ggacgaatta tcgacatttc tgaagatatg gctgttgtac aagtgtttgg
    18121 ttcgacgaat ggcttagacc tcgatgaaac aagtatagag tttttagaag aacccatgga
    18181 gttgcgtgtc ggggagggct tactcggtcg tgtttttaac ggacttggaa aacccatcga
    18241 cggctacgat gagattattt catcgacaaa gcgcaatgta aacggcttgc ctttaaatcc
    18301 ctatgcacgt gaatatccga gagattttat tcaaacggga gtgagctcca tcgatggaat
    18361 gaacaccctt attcgtgggc aaaaacttcc cattttttct ggaaacggct tgccacacaa
    18421 tagacttgcc gcacagatta ttcgtcaagc aaaaatttta agtgcagatg aagaatttgt
    18481 aatggttttt gctggcatgg gaattaagta cgacgttgca cgcttttttg tcgacacctt
    18541 tgagcaatcg ggcgttttat ctcgtgttgt actttttcaa tccctcgccg atgctccttc
    18601 gattgagcgt attattacgc ctcgttgtgc gctgaccgcc gcagaatatc tcgcctttga
    18661 aaaaaacatg cacgtcctcg ttgttatgac cgatatgaca aactactgtg aagcacttcg
    18721 agaaatttca acgacgcgag gtgaagttcc gggcagaaaa ggctatcctg gctacttgta
    18781 ttcggattta gctgaattat acgagcgggc aggaaagatc aagggctcaa gtggttctat
    18841 cacacaaatt cctattttaa ccatgccgaa tgatgatatt tctcatccca ttcctgacct
    18901 tactggttat attactgagg gacaaattgt attggatagg gaaatgtatc aaaaaggtat
    18961 ttatccgcct gttgcgggac ttccgagcct atctcgtctt atgaaagacg gtataggtga
    19021 gggaatgacg cgagatgacc acaaggatgt ttcaagtcag ctttttgctg cctattctaa
    19081 ggtaaaatcg attcgtaacc ttgctgcgat tattggggag gaagaattat cgcctctcga
    19141 taagcagtac ttagactttg ggaacaagct tgaaatggag tttttttctc aaaaagaaga
    19201 agaagatcga agtatagccg aaacgcttga tgctggttgg cagatgcttt ccttacttcc
    19261 acgttcagag ctttatagga ttgataaaaa actgatagat aaatacttgg gaccagagca
    19321 gctttcctaa aacaaagcat tagtttacct gagattttta taaacctaaa aaagaataaa
    19381 aatatttttt aaaaggtttt aataaaaagc gtagtatggt atactttttt aagaggcata
    19441 ggtatggcac gactaaatat tgcaccgaca aaatcaaatc ttctcgttat gaatgaacag
    19501 cttgctattt cacaagatgg atacgaactg ctcgaacaaa aacgagaaat tttggttatg
    19561 gaattgatgc gtatggttga gcaagtaaag ttgcttgagc aaagaatcaa tgcccaagtt
    19621 gaaaaagcct ttcctgcttt gcgaaaaatg ctccttgtta ccggtgttga taccgttgag
    19681 cgattatcgc aagcagttca gtacgatttt caaatccatg aaaaagaaat gtcagctggt
    19741 ggcatgagtt tttcttctgt ttctgttgcc ctacctaagc gtgaattgtt ttattctttt
    19801 gtcgattcat ttgctgaaac cgatgctgtt atggctgaat tttttgaatt gttaaagttg
    19861 cttacagaaa tggcttcgat tcgaacgata gcctggcgcc ttgcaactga ggtaaaaaaa
    19921 acacaaaggc gggtaaatgc cttggaaaaa atggttattc cgcaaacgaa ggaaacaaag
    19981 gaatatatag aaactgtttt ggaagaacga gagcgtgaga gcatctttgt cttaaaagcg
    20041 cttaagtcaa agcatgggcg ctcataggac aaggtctcta tttgttgcta tacgcgtaga
    20101 aaagaaaaat ctcgaagaaa atgtcttttc tgcatgtaca aaggagtagt atagtgaaac
    20161 atttatttga aaatgtactt attgtcatta atgactcaaa tgcctctata caagctgccg
    20221 aatttggaat actcatgtca aaatttaatg gcagtgcgct acacgctgtt tatgttatag
    20281 atacagcgac tctcaaacag cttaccttac aaaagatttt tgtaaaagaa gaatacgatg
    20341 agtttagtga aagtcttgag gcagacggga aacgctacct ccaatatgtg caagagctag
    20401 gtaaagcaaa aggcgtagct attgaaacag aattacgaag tggttctgtc tggtcagagg
    20461 ttatcactta tgcgaatgag atagaggcag atgtcatttt actcggtggc acaacaacat
    20521 cagatcctta tcaaagagat atgttcaatt cctcatatgg tgaaatatta ttgcattcaa
    20581 attgttctgt actatatgta aaagaagagt atgtcgacca actatataaa ttattataaa
    20641 cgaggaaaaa tatatgagaa ttgctatttt acatttttta gacgacgata aaacttcaca
    20701 taaaacaata ctacaaaatt tacagcgaac agcgaatgca aaagggcatg aggtgagtat
    20761 ttttagtgca aaaaaagatg cttataatct acgtttaagt atttacgatt atattagcct
    20821 gattattcca tcttctcctg tttttggttc aaaaatacca tcgagggttt ctgaattgct
    20881 ttcttcttgt ggtacaattt ctggaaaaaa agctgcagcg cttgtactaa agtcagggtt
    20941 ttcttcaaat aaaacagctc gtaatctcat gcgcgccatg gaaagggaag gactcaagct
    21001 cgattatttt gatattgtaa aaaacgcaga tcatgcaaac tatgtgggga aaaaattagg
    21061 ctagaaactg tgaaaaacta ctatgcgctt ttagaaatta aaccaactgc ttcaatagct
    21121 gaaataaaaa aagcctttcg agaaaaagca aaaaaactgc atccggattt aaacgcatcg
    21181 caacatacaa aagagtttca acttcttgtt acagcttacg aaacgctttc aaatgcaaaa
    21241 actcgttctt catttgactg ggctttttcc caaagtgaaa aacaaaaaca aaaacatttt
    21301 gactatcgcg tatggctttt aaaacgcgat gacgatgaaa gtcgcgtgaa actcattatc
    21361 tatgacctgc ttcgtcatag ggaagatgaa gccgttgcgc tctatcttaa cataaaaaaa
    21421 gaaaacccat ttttttgctt ttcatctttt ttaggtcggg aagcttttat ggacctcggc
    21481 tatatactag cagaagagct tttatttaga aaagaatatt acgatgcgtt tttacttttt
    21541 gagcgtatta tcaagatgga atgtgaaaaa gcttatttta agcattttta tccagaagtt
    21601 ttaaaacagg tcaatgctat cttacgtgca tcatttgtct cgaaactttc tgatgaactt
    21661 gcaattgatt tatatgagag ggcaattgac ctgcctctag ataaaaaata tggggcgctt
    21721 tgtttttgta aggcagggga gttgtatctt aaaatgggag atatggaagc agcaaagatt
    21781 tgttttaaag aagcacttct tttagatacg agcttaactg aaaaaaatac tacgaaaaag
    21841 ttttttgaaa cagcttgaaa tgaggtttta aaatttttat attagatacg gtagaatacg
    21901 aaaggaatga atacaatgat acatttaaaa aatgaaagtc aaatagatgg tattagaaaa
    21961 tcgtgtaagc ttcttgcgca attatttgaa gaactcatcc cagaggtaaa agcaggtatg
    22021 agcacagcgg atatcgatgc gctttgtgtc gactttataa caaagcatgg gggaaaacct
    22081 gcctggcatc aagaaggctt tcctggagct gcttgtattt ctataaacga agaagttata
    22141 catgggcttc catcgaaaaa aaagtttctg aaagagggcg atattgtttc tttggatata
    22201 ggaataaatc tcgatggata tatttctgat gcaacgaaaa caatcgccat cggggacatt
    22261 tcacctgaag caaaaaaact catggaagtt actaaaaaat gtcttgaaga gggcataaag
    22321 gcttgcgtcg tgggaaatcg tataaaagat atttcccttg cggtttcaaa agtagctgaa
    22381 aaagagggct acggcattgt aacagatttt tgtggacacg gcgttggctt ggcggttcat
    22441 gaggagccgt ctataccgaa caaaccacaa atgggaaaaa accctcgcat tcaagcggga
    22501 atggtacttg ctatagagcc tatgataaat ctcggtacag gggatgtgga agttttaacc
    22561 gatggctgga cggttgttac aaaagacaga aaaatatcct gtcatgaaga acatacagtt
    22621 gccgtttttg cagatagaac agaaatccta tcgattttat aagaatcttg taacattttt
    22681 ataaaaaaag tattttatta gtatctgtga ttactaaaaa caatcttgaa agtgtagact
    22741 ttcttaattg tcttattttg gaggtttagt atgaagacgt ggacaaaaag atttttttat
    22801 tctctccttt ttttaatcgg tattttattg tttgtttcat attcttgctc aacaagcaaa
    22861 acaggttcga gtaaaactgc ttttgcagat acttcccctg aagtaataat tggcaaggat
    22921 aatcttgcgg ttcttgaagc gatgcagggt gcctttcgct ctatcgcttc tcaagtcctt
    22981 ccttctgtag ttgaactcga tgtggttgaa acaaggcaaa ggcagcagtt ttcttttggg
    23041 gacttgtttg atttttttaa ccgtggcgag gactcaaaaa atggtactca agaatatgaa
    23101 gcaagtggca tgggctcagg cgtcgttgtt cgaaaaatag cgaataccta ctatgttttg
    23161 acaaataatc acgttgcagg ttctgcaaaa caaatttcta ttaaactaaa cgacgggcgt
    23221 atttttacgg gaacgcttgt tggaaaagat gagcgaaaag atatagccct tgtttcattt
    23281 gaaacaaagg aagatgttcg cgttgcacaa cttggagatt cagatttagt acaacaaggc
    23341 gatatttgct ttgcgatggg cacaccactc ggctattttt cttcgattac gcaaggaatc
    23401 gtatcggcta ccgggagagc aggaacctat gtaaataata tttcagattt tattcaaacc
    23461 gatgctgcga ttaatcaggg taattccggt ggtcccttgg taaatattta tggtgaagtt
    23521 attggtataa atacttggat tgcaagtcag tccggtggct ctcaagggct cggttttgct
    23581 attcctataa ataatatcaa acgtgcaatc gatgatttta ttgcaacggg tgaggtaacc
    23641 tacggttgga tgggtgtttc gcttgttgaa ataaacgatg aatataaaga agatttaggc
    23701 gttaaagggc aaaatggagc ctttctatca tccattttct taacttctcc tgcagcaaag
    23761 gccggaatgc aagctggtga ctatgttatt tcactcaacg gaaaggcagt aaagtctgtc
    23821 gatcaactcg tgcgcgatgt aggtgatatt cctgttggaa aggaagctga gtttaggttt
    23881 attcgtggaa aaaaagaaat gacgctaaag gtaaagattg aaaaacgaga tgaaaagatg
    23941 gtgagcgact cttcgaaact ttggcctggc tttattgcaa accccataac tgatgaaata
    24001 cgtactcgtc tttcgctcga caaaaaaatt gcaggtgttg ttatttcaaa ccttgaagca
    24061 aagagccccg ctgcagcgat gcgcctccaa gcgggtgata ttattacagc agtaaatgat
    24121 aaggcggtaa aaaacttagc tgagttttat gctgctttgg atactgtcgg ccaaaaagaa
    24181 atctggttcg atgtgtattc caacgggcat accttaacta caactcggta taagttttag
    24241 cgatgagtga aacaaaacat gtcaattttt ttgctttaga aaaagcatgt aaagaaaaag
    24301 gctgtccctt ttgcaatctc ataaacgaaa ggatatatcg gtatatcgac ggtatgcttt
    24361 ttgagcatgt ttccgatata ccctttcgcc gcgcctatcg cgctgccggt ggcttttgcg
    24421 acagacatgg gaaaattttg cttcattatc gtgatggtct cgctgttgcg attttggggc
    24481 aaaatatttt aagcgattat atagaagcct ttaagaaaaa aaaagctccg aagtttaaag
    24541 catgctgtcc tgcgtgcatc gaacaggaaa aaatagaaaa agatttttca agttttttaa
    24601 gtgaaaatca taaacacaaa gaaactcaag aacagcttac tgcatatttt actgcctccg
    24661 atgggctttg cgtacctcat tatgcaaaac tgctttcttt atttaatccg cctacctggt
    24721 taaaagaatt tcaggaagct cttttttcaa gccttaacga acgagctttg cgatttatcg
    24781 attattcagc ttggggaaat caagataaat ttgaaagcct ctctagagaa gaccaaatcg
    24841 tgtggcagga acttgcccgt gttttgcggg gaagcgtgga gtaaaaaaaa ctagtattcg
    24901 ttttcggaaa gcccagttgt ttcgaaaata tcttttgtga tgcgaagttt ttcctcccct
    24961 ataacctcac ctttccttgc gacgctttaa gttttagaaa tataaattta ttgtgaaaca
    25021 gaaaaacttt gctcaatgcg acatggatgt cgcagggatt tagtatgaca gttttgttta
    25081 caaaactgtc gaagttattt caaacatgga tgtttgaaat aacgtaagaa tcctgtacat
    25141 cctgttttct cgaatagttt ttttcaaaca cggacgttag aaaaaacgta tgcggcatgg
    25201 acgccgcagt gactaagtat gacagtttct gcaagcacac cagttggcgc caccctggtt
    25261 atgttttcga taaaattcaa aaaagatgca aaaaaaacgg tgcctttgtc gcctttacaa
    25321 gtttagtttc tgtacttact atgcctatta ttattgcatt ggttgcttaa atttttttta
    25381 tattgattgt tttctctttc atattatagt atactgcaat gcgtgataga tttagcagct
    25441 tttgaaaagg ttatacctca aagtagtaat gatttctcat ctcataaatc ttcaattgaa
    25501 gatacaaaaa tgtgtgcaag taaaacagca agctttatag aagaattcaa tgcacttgag
    25561 caatcaggtg ctctcagtga aattaatctt ttgcgcaaag aaaatcgtga gctagataaa
    25621 cttgtaaacg atgctgcgaa gctcatgtct ttgacgagtg tttctgacat gctcgattat
    25681 gttatatctc gctttttaga tagttttatt cccagttttt taggtttttt aattcagcca
    25741 cctcgtgggc aacatatacg gcagtattgt tataaaaact taaaacaagt aaatgaaaag
    25801 atccctacag atttttataa aacactaaaa aattattttt ttgatagtcc atcgcccata
    25861 atctgttcag aattgccgga gaaaaacttt ctttgtcatc ataaaaataa tggacttttt
    25921 tttaaaccag aacttatttt tccattacgt agtattggtg gtttatacgg agtggttgta
    25981 ttaagtgaaa aagtagtggg tggaccttat actagcctgg agcaattgta tatagatcga
    26041 atgatgcgat ttttagctgt tggtattcaa aatggtttgc atcatgaaag ttctataaca
    26101 gatccaaaaa ctggtttata taattatgat tattttatgc aacgtcttga agatgaagcg
    26161 tatagaataa aacgccacgg tggattagca gcacttataa tgatagatgt tgattatttt
    26221 aaaaacttca atgatagcta tggacatatg gcaggggatg tagcccttga aactttagca
    26281 gacgtgcttc gttctatagt acgagaaggc gataccattg ctcgttttgg tggtgaagaa
    26341 tttgtaattc ttcttgttga aagtgatgaa gattatgtct tagaggtctc agaccgcata
    26401 cgagatgcta tagaatctac tcctgttatg tgggaggatc aagccttatc cataactgca
    26461 agtttaggag tacgtcttat ttcaaaaaat acgcttagtg attcacgaag cctcattgta
    26521 gatgtagata aagcactcta tcgttctaaa gaaaagggac gaaattgtgt tactttctat
    26581 acttcaagtc tcttacgaag agcgactgaa tatttagaaa ataacgttta acagcgattg
    26641 caaatccggg ctatgtattt acgaactggt ctggagatgc tacagggaat gagcaaagtc
    26701 ttacaatcga taatatccag agtcatattt ataatgcctt agattctttg gcaaaggttt
    26761 ttaataatag tatgcagggg gatcttccag aaagcttaaa tgctgcggca ctcagtggct
    26821 tgatgatgaa tacaccatac tatacagata ttggctgtct tatggaaaca atagattctt
    26881 gtactactgg ggcaagttcg tatactgctg ctgtgaattc tgctttgagc tctgcggtta
    26941 aatatcatag aaattttaca tctaatggat ttgattatta taaatatact actggtttaa
    27001 ctatctttca taatatttgg gaagcgcaat ggctcaatat tgcttataat cctgcaactt
    27061 acaaggcaat tttacagttt ggaaaaaaca atgcctgggg taagtacatg caaaatatgt
    27121 ggaataaaac accacctaag ccagaaatag atatagaagc cgatagctat gagccagatg
    27181 gattagacga cgacccggta actaatattc tagggaaggg tattgcaaat aaacaacatc
    27241 atactttcca tcttcttgag ggtgatatag atgttatggc agtaaattta gaggccggaa
    27301 aaacctatac ctttatgacg gaggcaggtc ctcttggtgc ggatacctat atgattctgt
    27361 ttgatccaag tggtgcgtac atcgatgaac atgatgatat tgattatcca aacaatctgt
    27421 actcaaaaat tacgtatgaa gcagaaaaaa caggctatca tcttttaatt ctcgttgatg
    27481 aatatcaaaa atacggtgat tattacgtgt actatgatga aggtgatttt ggaccgagtc
    27541 ttgggaaaac attccgtaag tggacacccg atcgaaagga caaagagtcg tacaagataa
    27601 gtcttcccta gcttttagta ccaagagccg tccctttgga tggctctgta tcttcacatg
    27661 aggaataaaa aatgaaaaaa atcttagctc tatttgtgct tcttgttttg ttcttttctt
    27721 gtatttcaca aaaacaaaat aatgctgaac taaaggaagt agcggttaaa gaagagcttt
    27781 tacaattgga tgaggatatt cctacttcta ctgctattga tctagatgct ttgtctaagg
    27841 aaggtattaa aataaagggt agggtggaaa aaattgtcga aaatgaaaaa acctttagcg
    27901 aagtccgtta ttatgtacta attacagaaa aaaatacgag tcttgtattg tttaataaaa
    27961 aaggtttttc tgaaggtttt gaaaactatc tggacaaaga ggtctttata gaagcgtata
    28021 aagatatagg catgattggt tttaaacgcg aaaaacaagt agggcttttt gttctttcaa
    28081 ttcgggaaga agattagtat cgttaaaagg ggcgtgaatt tttcacagaa atcctagata
    28141 agctttgcct aagagttcca attctctttc tatcttttct tcatttgctt tttgactttc
    28201 ccacaaaatc taaagcataa aatacttgta cagagcgcaa taatcggtta ttatttagtg
    28261 tatagcgaat tatttcgcga gtttaaatga agtgtaacga ggattgcatg tacaaagaat
    28321 ttttacccta cgataaggtg cgaaacaact ctctcaaaat ggcagcaaga atccacaaag
    28381 agggttttat ccctgacatt atctatgtgt ctttgcgcgg tggagcctat gtggcaaatg
    28441 ttatctccga atattttaaa attgtgcgca aaaatgaccg gcctgtttta tatgcctcag
    28501 ttgttgcacg ttcctattct gatattcgac aaaatgataa ggtaatggtt gacgggtgga
    28561 cctattcacc tgaacattta cgtcatggag ataaaattct ccttgtcgat gatatttttg
    28621 atacgggtaa gactatcaat catttggtag agctcataca acaaaaaggt ataccgcgaa
    28681 acgacattaa ggttgcggta cacgattaca aatatttcac ctattatcca gagcaacttc
    28741 ctatacaacc cgattattgg tgcaggcgtt tcgatattac agagccaagc gaagatcgat
    28801 ggattcatta tatgagtcat gagcttgttg gcttaacaga cgaagaatta aaggaatatt
    28861 actacaaaga tgatcccgag ctcaaagcaa ttctcgattc cattatgaaa aagaagggat
    28921 aaatatgagg ctaagacgca tcacggtttt tagtttattt ttctttatgc taggtggctt
    28981 ttatgccttt gacaacctaa gtatacaaga agatgcgtct ctctacccaa aggcagattc
    29041 tgaagcagtt ttaagtccgg tttctgggac atgggcaaat tatcaaagcc ttgttttgga
    29101 cgtccctaaa gatgccattg cgctttactc ttttactggc gatgatccct tggagtttgg
    29161 ttttgcctac gatggtccag tgcgtatcga aaaggagggt gaggttgact tgcgtatcgc
    29221 ctttgtctat gcggacaata ctgttcttga aacgcaggtt tcctatcgag ttgttttgcc
    29281 aggcttgctt tctttaggag aagagggggc ttttttaaat caaaatcagc cttttatacg
    29341 agcaagtgca gacaatcctt taaaaattcc gtcagacttt ttatatagtt tagacgatga
    29401 taggctaccc catgcaaggg gacggactct ttttttagct gaaaatacat atgagcgtta
    29461 tctaccgctt ctagttgctg ttgacgacca agtgtatcgc tggatgcttt ttctagaaaa
    29521 aagtaatgaa tcaagtaccg aaaaaaaacc aagtgaggca agggcttttt ctacaaaact
    29581 gagtctctct aatcctgatt ttctttttcg tttgagcgat agacctgaaa gccctttggt
    29641 tcaaagcctt tttatatcgg ttttggaagg ggagtttttt tctaaacgta tttcaatcga
    29701 tgtgtatgat ggaaacgaat ttatagggag tcttgaaaaa atgctttctg taaataggtc
    29761 gctacccaaa aagcctgaat ttatttcttc agcgaagtct ttttattctc gagaggctgt
    29821 taaattaaag cttagccata acgaaacatt tttttatctc gtcgatgagc cagaaatctt
    29881 gcactttgat gataaaacag tttcttacca gatagcagaa aaaaaagagc cgacgcttgc
    29941 agagttgcgt tccggatata aaactaaagg cttggaaata gaatttccca cgcatagcga
    30001 agcggttttg tatcgtatct atgctattgc agaagatagc gagggtaatc taagcgatat
    30061 tgcaagctat gaatgtattg tcgatgcgaa tcagtattat atcgatccgc gaaaaaaaac
    30121 agagcaaagc gcaaaaaatg ctttctatcc tgccgatgga agtcctgatt tccccttttt
    30181 aagtctagaa gatgctttaa aaaaactttc aaacaaaaat ggtatacact tgcatctttt
    30241 agggaacgat gtgattcaaa acactttaca gataaaccag gatttttcac tcattgggca
    30301 taatgatgca gaattgcagc tcggttctga tgtttatttt gaagtaaggg atgcaaaact
    30361 gagctttgaa tctgtttttt ttacctctga ctttcaaaag aattcaaaac aaagcaaaga
    30421 aaaaatatcg tctttggctt ttcaaaaaag tcttttttct cttagcgatg cacaagtgct
    30481 ttttgaaaac tgtgattttt cttttttcca tccagggaat tttgtccttg ctcaggcgcg
    30541 caattctcgt ttagattttt atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30661 nnagatttat tctatattga ttttagttca aaacaaaagg ccgttgtgga taatactctt
    30721 ataaaaaagg accttgaatg aaaaggtggc tgattctttt tctttttttc tttgcatata
    30781 attcatacgc tcagagtttc tttttcgata ctcttttaga gctttcttct tactgggatt
    30841 cttttattgg cgcaaaagaa aaacgagata agtcgacaga aagcgactta gacgctgcct
    30901 catatgcggc gcgctatact gaaagcttta tcgctttact agaaagtttt ttggaccata
    30961 agctagatgt agaaaatgat gctcaaaaaa agagagctga aaaaatagat gattctcttg
    31021 cagagttgaa aatgcaaaac gaagacagag gctatgcaga agccttactt tatatggagc
    31081 ttttatttta tacaagctat gctcgagatt tttttataga agcagaccaa aaactaaacc
    31141 agttaatagc agaagttttt gaaagaaaat ttacaccaac atatatagca gaaaatgaat
    31201 atctatttct ctcgcgttct ttgttttata gcttacatgc gcgaattgaa aaacacgtct
    31261 cttcacattg gcttcctgac ttagaggata ctgaagtttt ttttaaggct tccttgcgtc
    31321 gtttagatag cggtttaagc gcaaaagaaa taagggctaa acaatattct gcactgagtt
    31381 cagtagaaag tttgtattca aactatcttg cgtttcaaaa atcgcaagat tctaaaaaac
    31441 gctttttatt ttcagaagaa atgttttata gctttttaaa taagccttct tttttgtatt
    31501 atctttttca tatggaatct tatttccctc ttcgagacgc ttttctttct tcagcagaaa
    31561 tatttttgga aattgatcaa ggtattatag aagaaataaa aaaagcagaa tttgattttt
    31621 tatatgacag ctctttttct tttttttata gatattttta caaagatctt tatgcaaaaa
    31681 ttcccgacga taaacgtaaa gaatctattg cccttgtgta taaaacaaat gacttgttct
    31741 atcaaagttt tatcttcgct gcaaaatacg gtttttctat cgatgctgtt ttaacagaag
    31801 ataatcaact tttatatagc ttgtttttat cggacccatt tttactgagc ctttatgacg
    31861 ccgaaactat gggagctttt tgtgacaatc ttatttttat acaaatgctc tctgatatct
    31921 atatcaatcg tataaatagg cttccttcta ttcaaaggct taaggggtag ttatggcaaa
    31981 aaaaaatcct gtttttcttt tttttatctg gctctctttt tttatccttc ttttttttgc
    32041 tggatttttt atctatcaaa aagaacttat tcctcaattt aatttctggc ttaattcttt
    32101 tttttataaa gaatctaagc caaatgcagc ttccttacca gagatagagc ccatacgtat
    32161 tgcatctggc gaaatcggtg aaatggaggc ttttgcatat aaaataagag atagtcaatt
    32221 cctcatcgat atagaagctt tttttcgtga ggattttttc gataatacaa actctttttc
    32281 tatgagctat tccgaagagc agaagactat gaagcttact ggaatgtttc aaagactcga
    32341 tgcggacttt gacccaaagg atgagtcaat tcgctcctgg acttctctcc aggcagcgcc
    32401 tcttagcgct ccttctttgt ttcaagactc ccttgttttt atcgataccg atctcaatct
    32461 catcgtcctc gacttaaaaa ctggtgctct aaaacaatct tttccaacac cagtctatcc
    32521 agaaggtctt gctcttgccg tacaagcgcc cctttcattg agcggtatat accaaagcga
    32581 tgacttagtt cctcagtata ttattaaggc tgcggatgga aatgtctacg cctttgcttt
    32641 ttttgatcct ctcttgtatg aagatgtaaa aactaaaatc tcgcttatct ccgatgaaaa
    32701 aaataaacat cctttttggc cttctcagga agtagaaaaa tccatgatgg aagcgactgc
    32761 aacttgggcg cagtatgctg ccgtgccaga cttcattgaa ccgaaactgc ttctgagtga
    32821 tggatcactt cttcgagtta atgcagaagg tatttctgtt attgccctta tgttcaaaga
    32881 agaagggcaa taccagatgg gtatgagcga tgcgagttct ctttttatta gcgcggatgc
    32941 ccttgcttgt tttttttcac agacagggga gctgctctcc gtttctgtcg attatgaatc
    33001 tcataaaccg caggtacaag tacttttaga agctgatacc ttgtattata tcgttcttgc
    33061 aaataagggg gagcttcctc taaacgatgt ttatttttcc tataaaaaaa taaaataact
    33121 atgatgagtc aaaaagattt gtttacagag caattagata atctgagcct ttcatttatc
    33181 tgcggaattg atgaagctgg tcgaggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33301 nnnnnnaaaa atcgattgcc ttgctattcc aaaggcagat gcgaattatc cttctgttat
    33361 ggctgcatct attctcgcta aaaatgaacg agatcgaata atgtgctcct acgcgaaact
    33421 ctatcctgaa tatttgtatg agcaacacaa gggctatccg acaaagcaac atagagaaat
    33481 atgtaaaaca aaaggtcctt ctcctattca aagaaaaacc tttcgttttt aatgtttttg
    33541 tattttactc gtccatattt cctggttttt caggcatgat aattgctgcg atgatatagg
    33601 ctaaaagtcc agagcccaca cctgcaacac ttaaaaatac aacagcgagt cgtatgaggg
    33661 taggatcaac tgaaaaatat tcagctactc caccgcatac gccacaaagc attttgtttc
    33721 tcgatttgta taatcgtttt tcactcatta tttcctcctc atttttttta ccattcgtct
    33781 gtattttcat ctctatcgtt catttttatc ttaatattaa atagtttaga attaatgtca
    33841 atgaatttat atttttttgg ggtttttatt tcgatttcga gaggaaagat ttcctcaaac
    33901 tcatcattga agattcctgc aatagtataa tcgcctgcga tttttgttaa ggcactacag
    33961 gtgatgaagc ttccaagaat gaataatact aaggctaaaa ttaatagggt ttttgaaatt
    34021 tttttcatac tcggctcctg tttctaaaag gtattgaaaa taaggacacg ataaagcgaa
    34081 caagtggggg aagcattttt ttacaaaacc aaattccaag cactaaaccg acaatgccaa
    34141 aagccatgag caagaggctg agtcctaaaa atagaaaaaa gctcggtgta aaaagtatca
    34201 tagaaaaaag agcaaaggtg ccgagaacga tacttgcaat catgagggca aggcctatta
    34261 ttattcctat aaagcctatg ctgagtgtta ctactacaaa ggcaaataat atgccaaaga
    34321 ttgagcagat aagtggaaac catagaggaa aagaaaaaac gagtatgagc aggactaaaa
    34381 gaattttatt gcctctgttt tcattttttt ggtctttggg catagtctta ttttttcttt
    34441 cttcagtttt ttcttgttga tctgatttgt tttttttccc aagtactgaa ggcagacaag
    34501 aactgttttt taggatatga gcagcaagtt cttctggctt accaagctcc tttataacgc
    34561 gattatgatt ttcttctccc gcttcttcaa aatatgagcg ataatattcc attacatttt
    34621 ctttttcttc taaaggcagc gcagctaatt ctatttcgag taatcgcaag tattcttctt
    34681 tctccatata atctcctaat ctactgtatt taaacctaaa agaactgttt caattttctt
    34741 tctatactta atccattcgc gtctgtaatt atcgagttta atcataccat tcgatgtaat
    34801 tctataataa cgtcgatttc taccagaaaa ttcgccgtca taggtttcaa gacagttttc
    34861 tttttgtaaa cgcctcaaga ctggatacaa actgctttca gaaataaaaa gtatttcttg
    34921 gacatcttgt gttattttat atccatagga atcttctttc gcgagaagtg ataaaactat
    34981 actatcaagt agggctgttc ccgaactaaa ttgcatctaa agccttttct attatgatat
    35041 tatactcatt atagtatcat gatttttata aatgtcaagc acctgttata aaaatagaga
    35101 gaagcatttt gttattttaa aaataaaaaa accaataagt taagaatact tattggtttt
    35161 tctttgattt ttttagaagc cgttttgacc gcttccagct tttaagctat tattcttata
    35221 gccttggtct tttccaactc caacaggtag tgcaccactt ggtgtgttga tcccggcaat
    35281 tgttcgagca agggcgtgaa tagagcattc agcaaccccg taggtcgcaa gcacagtgtc
    35341 tatctcatca tcgataacaa ctaagtcttg tgggctacgt aaaacggtaa ggatagtttt
    35401 ttcatttttt gcgtagctaa agcatttttg gatgagttca agttgttttg gatactgata
    35461 ggcattgtat gtacaaagta caatgtagtc tgccataaag gcccagtcgc ttgcaccgag
    35521 gatttcggga tcagatgggt ttaatccaaa tacgtactcg cttacattgt caaagtattg
    35581 gctaaaaatt tctttcatat tgtgcagata aaagtttgta ttatctgaca tcatgaggcg
    35641 tccaaagctt gggcgaacga tagcaattcg tgcctttgga tctattttcc gtaatggtaa
    35701 gagatttgat ttgtttcgaa gtaaggtgat tgaagcatct gctgcatctt gaatagcttt
    35761 tgtatgcgct ttgcttgcaa caatttttgc agcttttttt tcactcaaga gattggtatt
    35821 aacatatttt gccttgtatt ctaggacacg taaacaggct gcatttaggg tttcttcact
    35881 gagctcgcct tttctaactg catcagcaat ttgaatgtga gcatcataga caccacctga
    35941 gacaataatg aggttaacgc cagcatttat cgccttaacc atggcaccgc caatgccata
    36001 gacgttttta attggctcca tttgaatatc atcggttaca attaaacctt caaaatgaag
    36061 tttgcctcgt aggtagtctt ggaggattgg ctttgaaagt gttgcaggag ttttagtttc
    36121 atcaagcttt ggaaaagatg cgtgcaaggt catcatcatg ctgacaccag catcgattgc
    36181 tcctttgaag ggaacccatt catgactcat aagtctttca aaatcgtgtg gcacttcgtc
    36241 gagttcaaag tggctgtcga tatcgacgtt tctttgacca ggaaagtgtt ttgcacaagc
    36301 agcaacgcct tcttcttgca gaccctttgt aaacgctgct cccagttttg atgcttgttg
    36361 tgggttgcta caaaaatagc ggttatcttt taccggaaga ccttcttcaa aacaaagatc
    36421 tacaacaggt gcaaaacaaa catctacacc gatagctttg agttcttttc cggtaacctt
    36481 tcccatttcg tatgcatgtt tttctgcatt ttcccgaatc gcaccgagtg ccatgtttcc
    36541 aggaccgatg ctaatttttt tggttattgg ggcgagttga ccgccttctt ggtctattgc
    36601 tataaattgt ggaattttat gaggagattt taatgaagcc gcttgcactt tttcagttaa
    36661 gtctcttagt tgaaggctat caactacatt acagcctgaa aaatacaaga tagagcccac
    36721 tgctcctgtt tctaaaagtt tttgcatgtc ttcgggtatg tcgtatctat cgacaaaact
    36781 agacataatc aactgtcccg ctttttcttc taagctcatt ttggacaata attcttttgc
    36841 gttcatcctt acctcgttta aaataattct tatcatagaa ttgataaaat atatcgccac
    36901 tataacattg aatagattat atttcaacta tttctagctt taacgtgaaa ataaattgct
    36961 cttttagacc tatttatact tgagtatagc aaaaaagtat tgtagtatgc aagtatgatt
    37021 acacatactt cttttattcc tgcggtgcaa aatttgtcta ccgaaacagg atgggtggaa
    37081 acacgaaatg cgctcataca ttttgagccg atgtttgaac ggcttagtct tttggctaag
    37141 gagcaattat caaaagctgg tttactttct tctgtgcaat cgccaaacca ggctgaactt
    37201 cttgtacaag gttctgtatt aaaaatatat ttacaagaag ataaaaatat tgcccatgat
    37261 gaagggtatg agctttctat tagtggtaaa actgcaaaat taaaggcaaa aactgaagct
    37321 ggaatgtttt atgctttgca aacgcttcgt cagctcgtat atagctcaga ggcaaaaata
    37381 ccctatatca ccgtaaaaga ttatcctgcc tttgaatggc gtggttttat gctcgacaca
    37441 agccgccact actatagtgc agattttata aaaaaaatga ttgaccttgc ctcttttcat
    37501 aaactcaatc gttttcactg gcatctcacc gatgaccaag ggtggagatt gccggtaaaa
    37561 gcttatgaaa agctcactga agtgggggca tggagagatg acccacgtta ttcttgggga
    37621 gcaataaaag gtggctttta taccgaagaa gaaataaaat ccgtagttgc ctatgctcgt
    37681 gagcgtcatg tcattgttgt gcctgaaata gaaaccccag gacatgcgag tgcaattttg
    37741 gcgagttatc cagagctagg atgtacgggt ggtccctatg ctgttgaaac acgctatggt
    37801 atttttaagg aagtgctttg tgcaggcaat gatgctgtat ttagcatgtt agaaaaagtt
    37861 ttcgatactg tttgcgaact ttttcctggt ccctatgtgc atattggggg ggatgagtgc
    37921 cctcacgaaa gatgggaaga atgcccaaaa tgtcaaaagc gtatcaaaga tgagggattg
    37981 gaaaatgcaa gtgaattgca aagttggata acagttaaag tttcaaaaat gctcgaagaa
    38041 cgtggaaaaa tccccattgg ctgggatgag gttttagatg gcacagaaaa acttgggctt
    38101 ccaaaaaatg ttgtcgttca atcatggcgg ggagtagagg gaggcattaa ggcttcatct
    38161 cttggtcata aggttataat gtctccttta actgacggct gctaccttga ctacaaacat
    38221 tttgatactg aagaagaacc tggaatgcat aaggttacga ctgttgcgga ctcttataat
    38281 tattcaccga taaaaccaga gatgacaaaa gaacaaagag aaaatgtgct cggcggacag
    38341 ggaaatatct ggactgaagt aatgtacgca tctaaaatag cggagtatat ggcctttcct
    38401 cgcttatctg ctatctctga atctttgtgg cttgaggaag agaaaaaaga ttttctctct
    38461 tttgcaaaac ggttgataaa acataaacaa agacttgatc gcttaaatgt actgtattat
    38521 aaggggcaat tatctgccca gtagaggtac tattaactgc ttatctatga gcttgtatga
    38581 ctatatgatt ccac
//
LOCUS       DAOSSW010000008        36872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_1260_length_36872_cov_7.422251, whole genome shotgun sequence.
ACCESSION   DAOSSW010000008 DAOSSW010000000
VERSION     DAOSSW010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 36872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36872
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1260_length_36872_cov_7.422251"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1990)
                     /locus_tag="PKW26_01565"
     CDS             complement(23..1990)
                     /locus_tag="PKW26_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ92289.1"
                     /translation="MQEKMNPFLNFITSGKYFEIRDESNIDLVVRYIIQNFLILLGGS
                     LLFGFAFSNYKIGNNIEAFVDFIMGLTTVAAFFILRTKASFKIPSFMTVVPFGVLCAF
                     YFYGGGTALSGIFWSFTFPMMAIFLLGRFWGSLLSSFLWVFMFICAFLPGFSPVQFAP
                     AYASRIVSVYALISALTLVYEQTKITKDRWVEVLTKSLKEERDTITAMKDNLKVGLFL
                     MDTDFIIQGQYSKALEEVLIEEELEGKSFIDFLSASLKDKELQTLRDYFEMVIHRSYD
                     AQMLEDINPIHEFRFVHPVSHEEKTLSTMFTALERDNGELYILASISDITKEAELRAQ
                     LADEESKRRSEMESMFEVIHVDPKVLFDFIEDADYEFNRINFLLKDRSKSSDKVVVEM
                     YQAIHAIKSNAVVLGLNSFAEKLHAFEEKVKELRDRDEAVEFEDILKLTFALESLMKI
                     EDSFKEIIGKIAQFSTEHKKVGENFVLIELLKQASIKTASVENKMVQFEAKKIDELLI
                     TGKYRRSIKEILLQLVRNSIVHGIESPEHRLEIGKEKEGLIRLKTERKENSLLVVLSD
                     NGSGIDYDKIAQRAVANNLIPESLSNDKKVLTNLLFSPGFSTASKADYNAGRGVGLHL
                     VKERLKEIGGTLKLKSENGKGTIFIISLPLN"
     gene            complement(2002..2472)
                     /locus_tag="PKW26_01570"
     CDS             complement(2002..2472)
                     /locus_tag="PKW26_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOQ92290.1"
                     /translation="MEKYIHPFIEVCENVFRDFIGAELTAERPFFVDKKDVHDWDISA
                     VIGLTGEARGAVVISMKKNLALKVTDMLTETEHKDIDDEVLDAIGEIVNIIAGNAKRG
                     LEEMFRLVISLPTIVRGVNHSISWPNEQTRIICIPFKIFDEETFSLLLAIEAAN"
     gene            complement(2487..2855)
                     /locus_tag="PKW26_01575"
     CDS             complement(2487..2855)
                     /locus_tag="PKW26_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ92291.1"
                     /translation="MKNVLVVDDSRIMRNIVKNTFTSLNIPCEFVEAANGNEALLQLS
                     EKKIDLVLLDWNMPQLSGIDFLKKIRAMPQYAELPVIMVTSEAARYNVIEALKSGATD
                     YIIKPVSEKSFKEKINKINF"
     gene            3012..4415
                     /locus_tag="PKW26_01580"
     CDS             3012..4415
                     /locus_tag="PKW26_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOQ92292.1"
                     /translation="MNNRAKKCPNQKTKTNSAKDMTVGSPLKLIFQFSIPLLFGNLIQ
                     QVYSLTDAIIVGRLVGTQALAAVGTTGPMNFLVFGFVYGLTSGFAIISAQRFGAKDEK
                     GLRQSVAVNIKLNLLSAISITSLSLLSSKPILLAINTPKEIFSYSLNYINIMYMGIIF
                     MIIYNTGASILRALGDSKSPLYFLIISAVLNIFLDIVFILYFQWGVAGAAWATIISQG
                     ISGILCVVHIIRKFPILHLTKKDFERNPYFTFQHLKIGVPMAFQFSITAIGVIILQGA
                     LNLFGANHIAGYTAAQKVEQLIGIGAGSFGTTMANYTGQNLGARRVDRIKAGVLSGSF
                     LTLIFAFFGMFISLTFSSQLTSLFIDTTTSEGAIALEASKIYLRGTAPFYPALFLIFI
                     FRNTLQSMGRGFVPLLAGVAELVARFIGALFFPMALGFLGIVLAGPFAWFAAVIPLAI
                     AYFWTIPKEHLYLDPLP"
     gene            complement(4375..4869)
                     /gene="ilvN"
                     /locus_tag="PKW26_01585"
     CDS             complement(4375..4869)
                     /gene="ilvN"
                     /locus_tag="PKW26_01585"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOQ92293.1"
                     /translation="MKRYVLSVLVDNHAGVLSRVSGLFSRRGYNIDSLVVGETHNPDR
                     SRMTIVVQGDQYILEQIEKQLSKLVEVVSIEQCNLENTIVRELALIKVNAKGSNRIGI
                     IETANIFRAHIVDVGVDSLVIEATGSEQKIASLIRLLEPFGILELVRTGLTAMDRGTS
                     VLSE"
     gene            complement(4940..6898)
                     /gene="aroF"
                     /locus_tag="PKW26_01590"
     CDS             complement(4940..6898)
                     /gene="aroF"
                     /locus_tag="PKW26_01590"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOQ92294.1"
                     /translation="MVIVLERGIPLRDRENLKSFLERNHFKVNEIRGEEETIFGAVGK
                     MSIDPREVEILPGVDRVIPISKPYKMASREFKKENTIVEIPNNWGQIIKIGGTRVTAI
                     AGPCAVESREQMMEIAKRVSSAGAVLLRGGAYKPRTSPYAFQGMGEEGLKILKEAGDA
                     YGLPVATEIVSVEHIPVMKDLVDVYQIGARNMQNFELLKRVGELEKPVILKRGLSATI
                     EEWLMAAEYLLSSGTNDVILCERGIRTYEKATRNTLDLSAIPVLRSMTHLPIIVDPSH
                     AVGIRDKVPPLALAAVAAGADGVIVEVHQNPDKALSDGPQSLYPEQFEKLMRDIEVLS
                     PVVGKEVSRIRSKPKTSEAIAGSDGAKTSFSCAYSGTRGAYAEQAILHYFDACNERLP
                     QKSFREVFQAVRDGRADYGMIPIENTLAGSVYENFDNLLRFEDISITGSIKLRIEHAL
                     IGLEGSQASDIKTVFSHPQGIAQCADFLADQPWDVVETSSTSDAVRIVKEKNAKENAA
                     IASIAAADAFKLAVIKEGIEIDPNNYTRFILISATNTNKSSVDRKGFSTNESSTAKGK
                     PMVSLSFSTKNEPGSLLRCLTVLHYLGLNMTRLESRPVQGEPWTYMFYTDIALTIGQD
                     SRLIDTAVEELKAVSEKVRVLGKYYEIN"
     gene            complement(6911..9412)
                     /gene="thrA"
                     /locus_tag="PKW26_01595"
     CDS             complement(6911..9412)
                     /gene="thrA"
                     /locus_tag="PKW26_01595"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HOQ92295.1"
                     /translation="MVSLKFGGTSMANAKRMLNSASIVLSRSEKEATSLVVSAVAGVT
                     NLLQESIDTVLTQEKPDIHLLAMKIEIIHRDIIKDLHLMANENLYNELLTVVDALCKE
                     YTLLLNAIKILGECPDSVTAKILGLGERFSAPIMKAILESKGQKVFLFDSRDYIIATG
                     DLLEGDPDFQATYDAFAKIREEKKDKNTVYLFPGFICGWKKDINSPVQMGLLGRNGSD
                     FSAAIVAHAMEARKLEFWTDVDGVYTADPKIVHDAIVVDNMSYEEAMELSFFGSKVLH
                     PKTLTPLASKNIEVWSLNSYNPEAPGTRIGKGPFEFDYSTSGAVRGITCLKNTAMISV
                     SGAGMRGKQGIAARIFSAVSRAKVSILLITQSSSEYTITFCVKQKDTEKTRFVLNDEF
                     EMEITEGLINPIEVQENCAIVSIVGDGMKKKRGVAGTFFDSLASRDINILAIAQGSSE
                     RSISVVISGEYGDIAVKVAHLFFFNQAQSIEVFVFGAGAIGGCLLDQIRDQKEELLTQ
                     KIDIKVVAIATVSSMMMDEQGLDLTNWRSDLEASTTETNLQGVLDFVQAAKPLNPIFV
                     DCTASDDLPNQYLDIFKAGMHIATPNKRANSLSMDFYKSLRTVANTQHRRFLYETNVG
                     AGLPIIDTLQNLFKSGDSLTGFYGIMSGSLSYIFGRLDEGASFSQAVLEAREKKFTEP
                     DPRDDLGGMDVARKALIIAREAGYELEIEDVIINRVFPEDFDDTGSVDAFIQNLPKLD
                     SYFAEKMAALKKENKVLRMAGFIEDGKCSVGMLEVGPDHPLYPIKDCENAFVFHTARY
                     NPIPLTIRGYGAGAAVTAAGVFGDILRTVSWNLEA"
     gene            9570..11642
                     /locus_tag="PKW26_01600"
     CDS             9570..11642
                     /locus_tag="PKW26_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOQ92296.1"
                     /translation="MNVAIAFDISALSLFTVILLLFINKRIAPTQTNRVFILFVLCLF
                     FTSFFSLASLLHKSYTFISFIPFDFFTITNHALKISIPLLFSYYIFTLVASQKKTSRR
                     FIKYTLLAPLIYSIFALTAFIFNLQASIAQAFYLLYTFIFLLQNVLFLKKHRSIFPKQ
                     KTIVLLSFVVLFTLVLNAEIYLASFSMGNIIFALSILVLLFTTHRQEETICNISLLYN
                     QTAFSSIYANYFEKKIPFTIVTIVLDDLFYLLKTLGIQQINAIIKETAFFLNDIAKNA
                     LIFHFNDGKFCATFNDTSEEAYLTFIEKIKERFAKAWQIDLIEIKIFPRLALIACPKD
                     AESIEDVIEILEILQHDEKYKKEGLLYASQIDTSYRLKTAFLDHTVKNALAENRIDVY
                     YQAIFSTEKNRIIGAEALVRMQDEKGRYISPEDFIPIAEKNGTILRIGEYVYESVCRF
                     INETDLAFYGIEKMDVNLSVVQCMQENLSEQFLMIADLYKIPYEHLNFEITETAAIFS
                     KKIFEQNIQRFAEAGIELSLDDYGSGYSNINYLINLPFNMIKIDKNIVWKAFLSDKSY
                     TALSFTISMIKALGMTVLAEGVETYEQAQQLTKLGCNYLQGYYFSKPVPKELFLSLLI
                     QNQTENEILEFIDSRNAKTQTKKILHDSIKELQSINKNTSQKSAETEDFDVLELEELE
                     EIEELKEI"
     gene            complement(11650..12126)
                     /locus_tag="PKW26_01605"
     CDS             complement(11650..12126)
                     /locus_tag="PKW26_01605"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOQ92297.1"
                     /translation="MSLFFQSQNTSLLWNFFSAFMATGVFALFFNANKYDVLWGALLG
                     GIGWTIYKWILLSGGSILYAYFLGAFFVALLSEILAIIIKNPATVFLIPGILPLVPGG
                     GMFFTMRAFVSGSVETALELGYTTLGAAGAIALGIAIVSVIARVYGKIRYSFSKKA"
     gene            complement(12123..12935)
                     /locus_tag="PKW26_01610"
     CDS             complement(12123..12935)
                     /locus_tag="PKW26_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOQ92298.1"
                     /translation="MEKKGDANLFITNESRLEHKEVLAISIRSASMILENGGETYRAE
                     ETATHTAISLGAKTATAFVTPTVVQVSYTDSKDSFHTAFRRVTRREVNLKKISRVNEL
                     SRRLAQRKSLAKPGQIDFVLSKIDTNAEYPSWFIILMGALSGFFFSFMFGGRLIDAVL
                     SLCIGLGLRLFLKALEKIQLNSFIISLFSGAIVSIFVELIDFIPLAFTQEIILTATLM
                     QVVPGLALVNAIRDLIAGDLLSGNARLVEAVMIAIGLSVGAVSGPLLFGGFL"
     gene            complement(12938..13756)
                     /locus_tag="PKW26_01615"
     CDS             complement(12938..13756)
                     /locus_tag="PKW26_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOQ92299.1"
                     /translation="MTDILLNAVIASILSAILFGILGSLVSVRRIASLAGAISHAVLG
                     GIGMAVFFSVSGRIPFFTPLAGAFLFAILSALIIFFVSTKSKQREDTVINAVWAIGMS
                     IGVLFMAKTPGYTDPSSYLFGNILLITKMDLLLLAILDLIVLFFAWLFYPQILSSSFD
                     ENFARVRGVKTELVFFVLLIIIAISVVLLQTFVGLVMVIAMLTLPSGTASYMAKSLSS
                     MMVFASIFSLLFSLSGLALSWTLDLPSGAVTVVFAGFVFLLFTLFHFISKKRRT"
     gene            complement(13749..14579)
                     /locus_tag="PKW26_01620"
     CDS             complement(13749..14579)
                     /locus_tag="PKW26_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOQ92300.1"
                     /translation="MKKASRQSPFLEKQDIDEGQKTIAFRFNDVSFAYGSVSVFENIS
                     FHVHENEFVALVGANGAGKTTLLKLLLGLEKPQKGSVELFPSTSKKREELIGYVPQYM
                     DFDSSFPISVYDVVKMGTVKGLGKKISKEEKAAIDEALVLSEVSNLAHRPYAALSGGQ
                     RRRVLVARALASQPKLIVLDEPTVNMDSESEKRLFETLRKLKGQSTILVVTHDSQFVS
                     SLTDVVLCAGEKDEVGGFRSVVKHTTSPKLSEVDLSATGLVRVNHNEIIDDRGCCDHD
                     "
     gene            complement(14576..15523)
                     /locus_tag="PKW26_01625"
     CDS             complement(14576..15523)
                     /locus_tag="PKW26_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOQ92301.1"
                     /translation="MKKTILFLMTVLFLFSLQNLFAFGSRDTGGKTKPLVAVSILPEA
                     FFLQEIAKDRVDILTLVGEGQSPHAYEPKPSQISKLTKADAWILSGLDFEEGFVPKIQ
                     KQIPNLSIIDITKTLQYRNFSPGEQDSHQHDDDAVIDSRDISVHSQERVLSSRVDKHT
                     WLGRTNAKLISREIYLSLIAIDPENTNFYKKNFDSLIFLIDKTFNEIAVDLQDLQGKI
                     VFVFHPSFGYFFDEFGLIQIAFEVDGKEPTAKQLAGLVDLVKEENARAIFVQKQFSKQ
                     AAEKIAKQTGARVLMLDPLSQDYIQNLYAMAKTIKEAYR"
     gene            complement(15573..16541)
                     /locus_tag="PKW26_01630"
     CDS             complement(15573..16541)
                     /locus_tag="PKW26_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOQ92302.1"
                     /translation="MMKVGILGAGRIATKMAETLNAMDDAKSYAIASRDKDRAENFAK
                     KYEIPQIYGSYQTMLEDKDIDLVYIATPHSHHFEHMKLCLEYGKHILCEKSFTMNAKQ
                     AREILALAKEKKLLVAEAIWTRYMPMRKTLDEILERKPVGEIYALTANLAYPVTHKQR
                     VVDLALAGGALLDVGVYTINFAMMAFGHPLSVDGTCIKTKTGVDETNSISLVYEDGRL
                     ASLQSSISCTSDRRGLLFGTKGFIEFENINNCEGIKVYDSEYRLIEQYFPPKQITGFE
                     YQVRACKRAIDQGLLECPEMPHSEIIKVMEVMDTLRAKWGIVYPGE"
     assembly_gap    16627..16726
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16727..17869
                     /locus_tag="PKW26_01635"
     CDS             <16727..17869
                     /locus_tag="PKW26_01635"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOQ92303.1"
                     /translation="KKQIDIKGYCVSKEGRKIPISVSTALLKDKRGRILGGAETFRDL
                     SEIENLKKELKRASLGKLESKNASMQKIQDMLPSLAESLLPVLIQGETGTGKEIVAKS
                     LHQLSPLKDGPFVAINCSAFPESLLESELFGYKKGAFTGADKDKPGRFALAENGTLFL
                     DEIGDISLNLQVKLLRVLQEGEYEVIGGRSPIKTNARIISATNRKLEELIEAKKFRMD
                     FYYRINVIGLELPPLKERTEDIVDFAELFLDGAFFRKNPTSDENPYFFSAEVLRYFYN
                     YPWPGNIRELENTVERMLALARSYELDKKLLPESFFQEKKKHEGKQGDDLTSIKKIQE
                     KAEKKMLIKCLEKNNWHKGKTASDLEIDTSTLWRKLKKYGIERATI"
     gene            complement(17905..18378)
                     /locus_tag="PKW26_01640"
     CDS             complement(17905..18378)
                     /locus_tag="PKW26_01640"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOQ92304.1"
                     /translation="MNLVKTDDYPEKAASFFYAGYNCAQATALAFAEFFDLKTTDVQK
                     AMAGFGGGFSGRRETCGAISAMVYILGLAHGDYDPNDNDKKTVFYELVRSAIEDFEKE
                     FGTTICKNLLEEYDINPQKNPQVRDEAYYRSRPCALFIKKAARLIAKNLQRKELA"
     gene            18549..19526
                     /locus_tag="PKW26_01645"
     CDS             18549..19526
                     /locus_tag="PKW26_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOQ92305.1"
                     /translation="MKKAISIWNNRIAPVFDESQECIIIEEDYSKSEVQKLPLGLEDK
                     IQFLLDNEVGTLICGAISRFAENQLLQNNIDVSPFVAGSVSEVISALEEGKLYTKKFS
                     MPGCPRPFVRRGFGMGRGMGCGAGRGPGAVFGHGRGFGMGMGSGRQEAGRGLGLGYGL
                     GPGRGLGLGYGRGFRQGAFYTEDENINYGQNDSPQEFSNQRIQGDRMKIAISATGKDL
                     NAKVDLRFGRAQNFIIYNTEDKSFVAIDNIQNLNAPQGAGIQSAQHIVDSGASVLITG
                     NTGPKAFRVLEAGNIEVFLSEAISVQEAIERYMSGSLEKTNQANVEGHW"
     gene            19532..20512
                     /locus_tag="PKW26_01650"
     CDS             19532..20512
                     /locus_tag="PKW26_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ92306.1"
                     /translation="MKNVLQIAIASGKGGTGKTSLAVALAKSLTLSKEKTLKQKEIYL
                     ADCDVEEPNAKLFFSAYFSEENKETKKYPTSLERVEVPIPQINHELCTLCGKCVEFCQ
                     FNALARVGRVLVFDELCHSCGGCTLVCPTQAISEVPHSIGSKTQKTLPHKEKDIHFIE
                     GKLDVGKALSPPIIRNVKKTLAEKTKDKNAIIILDSPPGASCPMTTTIDEVDFVILVT
                     EPTPFGLHDLKIAVETVRQVGYPFGVVINRSDAGDSRVIEYCESEKIPVLLEIKEDMD
                     IAKAYSIGKSIVETKSEYIELFIGLYKKIEELVEKKNIFQKPAITTGVKL"
     gene            20509..21456
                     /locus_tag="PKW26_01655"
     CDS             20509..21456
                     /locus_tag="PKW26_01655"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ92307.1"
                     /translation="MSEKKLKEIVVISGKGGTGKTSFSLALLDALSQNKKIVAADCDV
                     DAADMHLVLSPEIQKSEVFISGNFAHIQQEKCSRAKGSSCSVCYDLCRFDSIKKIESS
                     EIPEAKSASNPIRFSIGENDCEGCMLCVEFCPEKAIVFPERKCGKLFVSKTRFAPLVH
                     VALDIGSENSGKLVTEVRKKAKSLAEETNSDYLIVDGSPGIGCPVIASITGADQVLIV
                     TEPTLSGMSDLKRVAGLVKHFKIPHAVIVNKATVNLDISQEIESWCSKNNISYLGEIH
                     YNKNVTLAQLQEKTIIEYEKEEVEKPFTSFIQKIIKDIL"
     gene            21453..22226
                     /locus_tag="PKW26_01660"
     CDS             21453..22226
                     /locus_tag="PKW26_01660"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOQ92308.1"
                     /translation="MIDLVLMPTVKKKYVVMSGKGGVGKSTVSVNLAVSLALQGKKVG
                     LLDVDIHGPSIPTMLGLAGTKLTGSEESIDPVLVDGIPNLKVMSVGFLLESEDAPVIW
                     RGPMKNSIIKQFLKDVTWGELDYLIIDCPPGTGDEPLSVVQLLGKADGTIIVTTPQDV
                     ASVDVSKSIKFAEQVKIPVTGIVENMSGFVCPHCNEVSYIFKEGGGKKLAEEYKVPFL
                     GAIPIDPALGEAGDSGKPYVAQFKDSIISRTYEEMTKVL"
     gene            22256..22594
                     /locus_tag="PKW26_01665"
     CDS             22256..22594
                     /locus_tag="PKW26_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOQ92309.1"
                     /translation="MKIAIPVANGKLNMHFGHCEGFDLFEVDTKTKTLGEKSYIQAPP
                     HEPGLLPRFLNEQGVSDIIAGGMGARAQELFVENGIKVLVGAPCEESAKLAQDYLDGK
                     LRPGSNVCDH"
     gene            22613..23500
                     /locus_tag="PKW26_01670"
     CDS             22613..23500
                     /locus_tag="PKW26_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ92310.1"
                     /translation="MIITILADNRFCNRGGSANKNQDLLTEHGFSCLLETNDGQTMLF
                     DLGQKVFIHNAKVLGKNLKELDALILSHGHYDHSDGLSAFFTENTKTKLFASKHIFTD
                     HFSKKTGTARFIGLNEKNKNIIEKLPKTQKILFSKQSLVCKYKSGTNKHRIEVHGAIK
                     QKHPLEKPSPLLFADADCTESDTMLDETILAIETDKGLFLITGCGHAGFINICETIRK
                     RTRKKILAVLGGFHLEGVSKERLKATRDYIKKTGIEKIYPCHCTGEKEIEWLEKELPG
                     IAQKVCVGDLIKLDSFEEN"
     gene            23576..25768
                     /locus_tag="PKW26_01675"
     CDS             23576..25768
                     /locus_tag="PKW26_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92311.1"
                     /translation="MRIKQESSAHRWKFFRAGNVAQVVLETDDDLRHLAELDQKLWMV
                     LSMPCKGVQLSEKTLRLLDSDGDGSIRPPEILEAVSWICSTLKKPSVIFEEGSDLELE
                     NIQDAELQASAAFLLKALKKEDETSISYEDAHKRSEAFANLRFNGDGIIELEIIKDAL
                     LKEAASKIYAAQKESPAESSEEEIPSINRNMIESFSENVKNYFSWLENLERAFESIQL
                     NQTIEEKNKNLEVYIATIQAFIAVKDKIDDYFLRCQFVEFDSRTESILQGNENELEQI
                     SKKLLSQNDEILETFPISLVSNTERLSLHEGLNPLWKERVKNFVERVYHKIPLSDKKD
                     TDKFETLDKKTWEKICETFNPIVAIYEQKPQTNLSELRSEELKNLEDALPALLALVKK
                     DEDYAKERCLVDSLAKLLRYRRDLGIVLKNFISLEHFYTNANSVFQAGSLYIDGRTCH
                     LCIDIVDDEKHASLAALSGAYLVYCDIFRLDQAPKKIVAMLSAGDNENMIVGRNAIFY
                     DRNGLDWQAKVRRIISAPISVGQAFWQPYKKLYRSIEDQIIKRTQTSEDAIFTKINQG
                     ENLLQNNTVQTKENTKTPKKLDLGAIALIGTAIGGISALVSAFFQSLFGLGLWLPLGI
                     AAIILLISGPSMILASMKLRKRNLGPILDANNWAINSMARINIPFGSSLTELSSLAPG
                     SLVGSKDPFKGKSPKPKIILGLVIVIAIAAIVYFFPSILGFVKKLRYF"
     assembly_gap    25824..25923
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25924..27671)
                     /gene="ilvB"
                     /locus_tag="PKW26_01680"
     CDS             complement(<25924..27671)
                     /gene="ilvB"
                     /locus_tag="PKW26_01680"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOQ92312.1"
                     /translation="METKIMTATKTRTTSQTKTETQEIQKLTGAQVVIQCLLEQGVDT
                     VFGYPGGAILNIYDELYKSSDKIRHILTAHEQGACHAADGYARVSGKVGVVMATSGPG
                     ATNLVTGIATAYMDSVPLVAITCNVPTSLLGKDSFQEVDIAGITMPITKHNYIVRDIT
                     KLADTIREAFEIARSGRPGPVLVDIPKDVTAAEVDYILKKIDPNIATSDAVKRSNQKT
                     PLPTKDDLEDFAAILSSAKKPFFFVGGGVVIAEATEAFKALAEKLNAPVSVTLMGKTA
                     FSQKHELSTGMIGMHGTKASNALANKCDVFVAIGARFSDRVISDPNKFAREAKVLHID
                     IDRGEINKNIPTTASLTGDINIILKELTELVKPRAKDEWNIETEELKKHIPAMYSKKT
                     RLHPKFVMEKINEVLGDETIITTEVGQHQMWVAQFYPFVTPKTFVTSGGLGTMGYGTG
                     AALGAQIAQPKQRVVHIAGDGSFRMNLNELATISHYQIPVIIIIANNGTLGMVRQWQK
                     MFYGKRYSSTTLDRGPDFVKLAKAFDIRGYRAADEKEFVDCFAKAVSEKKPCLIDCGM
                     DIDEMVFPMVPAGKPI"
     gene            complement(27675..29333)
                     /gene="ilvD"
                     /locus_tag="PKW26_01685"
     CDS             complement(27675..29333)
                     /gene="ilvD"
                     /locus_tag="PKW26_01685"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOQ92313.1"
                     /translation="MKSDNAKTGYERSPHRSLFRAMGYTDEELARPIVGIVNSKNEII
                     PGHIHLDTVVEAVKAGVRMAGGTPMEFPAIGVCDGIAMNHEGMKYSLVTRELIADSIE
                     AVSKAHAFDALVMVPNCDKVVPGMLMAAARMDLPTVFVSGGPMLAGRHKGKRLSLTDM
                     FEAVGAVAAGKMEASELDEMECSACPTCGSCSGMFTANSMNCVTEVLGMGLPGNGTIP
                     AVYADRIALAKKAGMAVMTMIEKNITARKIMNEASFNNALAADMALGCSTNTILHLPA
                     IAHEADIKIDLKKINEISERTPNLCKLAPAGNAFIEDLYFAGGIQALLKELDKKNLID
                     SSVMTVTGRTIAENITRAENTDPTILRPIENPYSQTGGIAVLFGNLAPNGCVVKRSAV
                     APEMMKHTGPARVFNSEEEAFEAIQKGTIKSGEVIVIRYEGPRGGPGMREMLSVTAAL
                     AGRGMDKEVALITDGRFSGATRGASIGHCSPEAAVGGPIALVQDGDSISIDINAYSIK
                     LNVSDEELETRKQSWQCLEPKIKKGYLARYAKLVSSADQGAILE"
     gene            complement(29362..30453)
                     /gene="leuB"
                     /locus_tag="PKW26_01690"
     CDS             complement(29362..30453)
                     /gene="leuB"
                     /locus_tag="PKW26_01690"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOQ92314.1"
                     /translation="MQKQIALIPGDGIGPDITREAKKVLDAIAKKFSREFDYVEVLAG
                     GAAIDATGGPLPKESLEAALASDAVLLGAVGGPKWDSLPGSQRPEQALMGLRSGMKVY
                     ANLRPAVLFKQLSDACPLKNEIVKDGLDILIVRELTGGIYFGERGRSEDGERAYDTET
                     YSKMEIERILKVGFESAQKRNKKLCLVDKANILESSRLWREVAHEMKKDYADVSLDFM
                     YVDNAAMQLVRNPNQFDVIVTSNMFGDILSDEASQITGSIGMLASASIGDSGPGLYEP
                     IHGSAPDIAGKDIANPLATFLSLAMMFRFSFGWEAEALAIENAINAILDAGWRTGDIA
                     GSDEKRKALGEKLVGTTKMGDLVVKELLR"
     gene            complement(30438..30947)
                     /gene="leuD"
                     /locus_tag="PKW26_01695"
     CDS             complement(30438..30947)
                     /gene="leuD"
                     /locus_tag="PKW26_01695"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOQ92315.1"
                     /translation="MKAEGKVFKYGDNVDTDVIIPARYLNTSDAKELASYCMIDIDAD
                     FAKTVEAGDIMVADKNFGCGSSREHAPLAIKTAGISCVIAASFARIFYRNAINIGLPI
                     LECPEAAAEIKAGDRVAVDFDSGIIINNTSGKRFQAAPFPPFMQEIIKADGLIKQLNQ
                     KVGDVCKNK"
     gene            complement(30998..32254)
                     /gene="leuC"
                     /locus_tag="PKW26_01700"
     CDS             complement(30998..32254)
                     /gene="leuC"
                     /locus_tag="PKW26_01700"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOQ92316.1"
                     /translation="MTQKILAAHAGLESVKAGDLIEANLDLVLGNDVTTPVAINEFDK
                     IGCKKVFDTKKIAIVPDHFTPNKDIKAAEQAKKVREFARAHEIENYFEVGCMGIEHAL
                     LPEKGLVVAGDCVIGADSHTCTYGALGAFSTGIGSTDMAAGMASGKAWFKVPSAIKFV
                     LKNKLSGWASGKDLILHIIGMIGVDGALYQSMEFVGDGIASIDIDGRFTIANMAIEAG
                     AKNGIFPVDDVTLAYLKEHGKKDWTIYEADPDAEYERVIEIDLATIEPTVAFPHLPSN
                     TKSIDEAKGLKIDQVVIGSCTNGRIEDLRVAASILKGRKVHRDIRCIIFPATQKIYLQ
                     AVREGLAEIFVEANVAFSTPTCGPCLGGHMGILAKGERALATTNRNFVGRMGHPESEV
                     YLSNPAVAAASAVMGVITDPKDLMGR"
     gene            complement(32296..33888)
                     /gene="cimA"
                     /locus_tag="PKW26_01705"
     CDS             complement(32296..33888)
                     /gene="cimA"
                     /locus_tag="PKW26_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOQ92317.1"
                     /translation="MNKLVEIFDSTLRDGAQGEGISFSVQDKLSIVKALDELGVAFIE
                     AGNPGSNPKDLEFFSEAKKIKLKNAKLVAFGSTRRKGLAVAEDVNVQSLLTAGTDTIC
                     IFGKTWDFQVTEILKASLQENLDMIEDTIAFFVEQGKTVIYDAEHYYDGYKANPEYAL
                     KTLQVAKKAGAKTLVLCDTNGGMLPDDLAKITEEAVKETGCTIGVHLHNDAGMAVASS
                     LMAVKAGARHVQGTLLGFGERTGNANLSTIIANLELKMEMPCLPEGCIENLTSICHRV
                     SEIANIALYNGMPFVGSNAFSHKAGMHIDAVTKNPTAYEHIIPEMVGNERRFLMSEVA
                     GRSLVIEKMKKFDSSIQKDSPITAKIVERVKELEHEGYQFEGAEGSFELLMRKNMGKY
                     KPFFDLDYYKVIVEEAVEGFPSNAFAQVKIGVEDEKSITAGEGLGPVHALDSALRKGL
                     EKFYPSVKDIRLTDFKVRVLDSQSATAAKVRVLIDSTDGEKTWTTVGVSADLIEASWK
                     ALVDSFEYKLIMDIERKFKTYL"
     gene            complement(33881..35434)
                     /locus_tag="PKW26_01710"
     CDS             complement(33881..35434)
                     /locus_tag="PKW26_01710"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOQ92318.1"
                     /translation="MSRQIKIFDTTLRDGEQAPGCSMNLKDKLEVAESLALLGVDIIE
                     AGFAIASPGDFKSVQAIARTIKGSAVASLARALEKDIDAAWDAVRDAENPRIHTFLAT
                     SPLHMEYKLKKTPDQVYEQAVKMVAYARNLCGDVEFSLEDASRSEPDFMYKVIEGVIN
                     AGASTVNIPDTVGYAVPEEFAQLIKNIRNNVPNIDKAVLAVHCHNDLGLAVPNSLAAA
                     MAGAGQIECTINGLGERAGNAALEELVMNLYTRKDYYNMTTNIDTTRLYPTSRKVSKA
                     TGVRCQPNKAIVGENAFAHESGIHQHGVLANRATYEIMTPESIGLPHNKMVLGKHSGR
                     HAFEERLSYLGFAVSEEDLERIFVDFKNLADKKKTVSDRDIEALVRGFGSGIPESFRL
                     DRFVINSGNSITSTSVVRLINKDGELIEKVSIGDGPVDASLNAIDKIVGGSEIKLEDF
                     SLQIVDAIDTGTDAQGESHVRISRDGRSWNGSGVSTDIVESVIKAYLFAINSMVFELE
                     QEKARRNGE"
     gene            35608..36828
                     /locus_tag="PKW26_01715"
     CDS             35608..36828
                     /locus_tag="PKW26_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ92319.1"
                     /translation="MQIIREKYIKKALEKGKTSTIVLLDGLRGAGKSQLLGEIGNRLK
                     QQRPPVRVLILNALTGIKNDRHLLEQARGLGAGISALLIDNAEYIEDLYTALHAILKT
                     YNTRIFLTGQRTSGLEKMIRNSSLPQSIIPVFPFSYKEFLLHQELRENHAAFLSYLSI
                     GGLPQSLLLPVNTKESKELRKLIADSFILENIIEPYAIRNPILIRRILEKIAKTSGDQ
                     VSFRSIKESLETREQSISNQSLIDYLNFCSEAKLLYKIPIVDIKTKKIIEAGYAWYFS
                     DLGMRNTFSAGNYYRLPFHLQKTENEKALENAVFLSLIDKGYTISKGRITKGSSFIEY
                     ITFIAEREGKKIYIQISPLSQNLQSQIQKKEALLSIKDAWPKYLVGSEEEDGLTKEGI
                     QVISERSLLLDGGI"
BASE COUNT        11392 a         7744 c         6651 g        10885 t
ORIGIN      
        1 tcttatttac ctagtataaa tcttagttca aaggtaaaga aataataaaa attgtccctt
       61 taccattctc agattttagt ttaagcgtgc cacctatttc ttttaggcgc tcttttacga
      121 gatgcaagcc cactcctcgc cctgcattgt aatcagcttt tgaagcagta ctaaaaccgg
      181 gtgaaaaaag aagatttgtt aaaacttttt tatcatttga aagtgattcg ggtataagat
      241 tgtttgcaac tgctctttgt gctattttgt cataatcgat accactccca ttatcagaaa
      301 gcacaacaag caagctgttt tcttttcgct cggttttaag acgaatcagg ccttcttttt
      361 ctttacctat ttctaaacga tgctcaggac tttcaattcc atggacgatg gaatttctaa
      421 ccaattgcaa aaggatttct tttatactac gtcgatattt tcctgtaatc aaaagctcat
      481 ctattttttt tgcttcaaat tgcaccattt tattttcaac agaagcagtt tttatacttg
      541 cttgtttcaa taattcaatt aaaacaaagt tttcacctac ttttttatgt tcagtgctaa
      601 actgagcgat ttttccgata atttctttaa aactatcttc gattttcatt aagctttcaa
      661 gtgcaaaggt gagctttaaa atatcttcaa attcaacagc ttcatcacga tctcgtaatt
      721 cttttacctt ttcttcaaag gcatggagtt tttctgcaaa actatttaag cccaaaacaa
      781 ctgcatttga tttaatagca tgaatagctt gatacatttc aactacaacc ttatctgaag
      841 attttgatct atctttgagt aaaaagttta ttctattaaa ctcataatct gcatcttcaa
      901 taaagtcaaa aagaactttg ggatcaacat gaattacttc aaacattgat tccatttcag
      961 atcgtctctt actctcttca tctgccaatt gtgctcgcag ttctgcttct tttgtaatat
     1021 cagaaattga tgcaagaata tacaattccc cattatctct ttcaagcgca gtaaacattg
     1081 tactcaaagt tttttcttca tgtgaaacgg gatgcacaaa acggaattca tgaataggat
     1141 taatatcttc gagcatttgt gcatcatagc ttcgatgaat taccatttca aaataatcac
     1201 gcaatgtttg taactcttta tcttttaatg aagcagataa aaaatcaata aaggacttac
     1261 cttctagttc ttcttcaatt aacacttctt cgagtgcttt tgaatattgt ccttgaataa
     1321 taaaatctgt atccattaaa aaaagaccaa ctttcaaatt atctttcatt gcagttattg
     1381 tatctcgttc ttcttttaag ctttttgtaa gcacttctac ccaacgatct tttgtaattt
     1441 ttgtttgttc atacacaagt gtgagagctg aaataagtgc atatacactg acaatacgtg
     1501 aagcataagc tggtgcaaat tgaacaggtg aaaaaccagg aagaaaagca cagatgaaca
     1561 taaaaaccca taagaaagaa gacaaaagag agccccaaaa tcttccaagc aaaaagattg
     1621 ccatcatagg aaaagtaaaa gaccaaaata tacctgaaag tgctgttcca ccaccgtaaa
     1681 aataaaatgc acacaaaaca ccaaaaggaa caactgtcat aaaagaaggt attttaaaag
     1741 atgctttggt tcttaaaata aaaaaggcag caacagtagt gaggcccata ataaagtcaa
     1801 caaaagcttc tatattattt cctattttgt agttggaaaa agcgaaacca aaaagtaagc
     1861 ttccaccaag taaaataaga aagttttgaa taatatagcg aacaacaagg tctatattgc
     1921 tttcatcacg tatttcaaaa tatttaccac tggtaataaa attgagaaaa ggattcattt
     1981 tttcttgcac aaaaagcctc cctagttggc agcttcaata gcaagcaaca aactaaatgt
     2041 ttcttcatca aatattttga aaggaatgca aataatccgt gtttgttcat ttggccatga
     2101 tattgaatgg tttactccac gaacaattgt tggtaaagag ataacgaggc gaaacatttc
     2161 ttctaaacca cgtttcgcat tgccagctat aatattgaca atttctccaa tagcatctaa
     2221 aacttcatca tcgatatctt tatgctctgt ttctgtgagc atatctgtaa cctttaatgc
     2281 aaggtttttt ttcatagata taacaactgc acctcttgct tcacctgtta atccaataac
     2341 tgctgaaata tcccaatcat gaacgtcttt tttatcaaca aaaaaaggac gttcagcagt
     2401 taattctgca ccaataaaat ctctaaatac attttcacaa acttcaataa atggatgaat
     2461 atatttttcc attatcttct ccttttttaa aaattaattt tatttatttt ttctttaaaa
     2521 cttttttcac ttactggctt aataatataa tcagtagcac cgcttttcaa agcttcgata
     2581 acattgtatc gagcagcttc actcgtaacc ataataacag gcaattctgc atattgtggc
     2641 attgcacgaa tttttttcaa aaaatcgata ccggaaagct gaggcatatt ccaatccaat
     2701 aaaactaaat caattttttt ttccgacaat tgcaaaagcg cttcatttcc atttgcagct
     2761 tctacaaatt cacaaggaat atttaaactt gtaaaagtgt tttttacaat gttacgcata
     2821 atccgtgaat catcaacaac taaaacattt ttcattttca actccaattt caatatctct
     2881 aataccttaa attgtattat agttttaatt atatgtcaat aaaatataaa aaaaactata
     2941 aaaacttccc ttgactttaa ttattaatag aattataaca aagggaagac atttaaaagg
     3001 atactactat tttgaacaac cgagctaaaa aatgcccaaa tcaaaaaact aaaacgaact
     3061 ctgccaaaga tatgacagtt ggctctccat taaagcttat tttccaattc tctataccac
     3121 tgctttttgg caatcttata caacaagtat atagcttaac agatgcaatt attgtcggcc
     3181 gactggtagg gactcaggca cttgcggccg ttggcactac aggaccaatg aactttttgg
     3241 tttttggctt tgtctatggg cttacttctg gatttgctat tataagtgca cagcgttttg
     3301 gagcaaagga tgaaaagggc ttacgtcaat cagttgcagt gaatattaaa cttaatcttc
     3361 ttagtgcgat ttctataaca agtctctcac ttttatcttc aaaacccatt ttactagcta
     3421 taaatactcc aaaagaaatt ttctcatatt cacttaatta tatcaatatt atgtatatgg
     3481 gtattatttt tatgattatc tataatactg gtgcttctat tttacgtgcg ctcggagact
     3541 ccaaaagccc tttatacttt ttaattattt ctgcggtctt aaatattttc ctcgatattg
     3601 tttttatttt atattttcag tggggtgttg caggagctgc atgggcaaca attatttctc
     3661 aaggaatttc tgggatactc tgtgttgtgc atattataag aaaatttcct atcttacatc
     3721 ttacaaaaaa agattttgaa agaaatcctt attttacgtt tcaacactta aaaataggtg
     3781 ttcctatggc ttttcaattt tcgatcactg caattggtgt aattatttta caaggtgcac
     3841 tcaatttatt tggtgcaaat catattgcag gctatacagc tgcacaaaaa gttgagcaat
     3901 tgataggcat tggagctggt agctttggta caactatggc aaattatacg gggcaaaatt
     3961 tgggtgctcg tcgagtagat agaataaaag ctggtgtgct aagcggtagt tttttaacgc
     4021 ttatatttgc tttttttggc atgtttattt ctcttacttt ttcaagtcag ctaacaagtt
     4081 tatttataga cactactact tcagaaggtg ctattgcact cgaagcatcg aaaatatatc
     4141 taagaggaac agcacctttt tatccagcat tgtttttgat ttttattttc agaaacacct
     4201 tgcaaagtat gggccgtggt tttgtgccac tccttgcagg tgttgctgaa ttggtcgctc
     4261 gttttattgg agcactcttt ttccctatgg ctttaggatt tttgggaata gtgcttgcag
     4321 gaccctttgc atggtttgct gctgttattc cactcgcaat tgcgtatttt tggactattc
     4381 cgaaagaaca cttgtacctc gatccattgc cgtaagacca gtacgaacaa gctcaaggat
     4441 gccaaagggt tcaagaagtc gaattaagct tgctattttt tgctcacttc ccgtcgcttc
     4501 tataacaaga gaatcaacac caacatcgac gatatgcgca cgaaaaatat ttgcagtttc
     4561 tatgataccg atacgatttg agccctttgc atttacctta atcaaggcaa gttcgcgaac
     4621 aatggtattt tcaagattac actgctcgat agatacaact tcaacgagtt tggacagctg
     4681 tttttctatt tgttccaata tatactgatc gccttgaaca actatagtca tgcgcgagcg
     4741 atcgggatta tgcgtttccc ctacaactaa actgtcaata ttataacctc tgcgactaaa
     4801 aagccccgaa acgcggctta aaacacctgc atggttatct accaaaaccg ataaaacata
     4861 tcttttcata caatactcct ttgacacctt tgcaaagcaa aatctctaaa atattctaaa
     4921 acaaaagtaa aaaattttcc tagtttattt cgtaatattt tcccagcaca cgtacttttt
     4981 cagaaactgc ttttagttct tctactgcgg tatctatcaa acgactgtct tgccctatcg
     5041 tgagggctat atctgtataa aacatatagg tccagggctc gccttgtacc ggtcgagatt
     5101 ctagccttgt catatttagg ccaagatagt gtaaaacagt gagacagcgc aagagggagc
     5161 caggttcatt tttggtagaa aaactcaaag aaaccatggg ttttcccttt gcggtagacg
     5221 attcatttgt agaaaaacct tttctatcca cggaagattt atttgtattt gttgcagaga
     5281 taagaataaa acgcgtatag ttatttgggt ctatttctat accctcttta ataacagcaa
     5341 gtttaaaagc atccgctgca gcgatggaag caatagccgc attttctttt gcattttttt
     5401 ccttcacgat gcgcaccgca tcagaagtag agcttgtttc aacaacatcc caaggctggt
     5461 ccgcaagaaa atctgcacac tgagcaattc cctgcggatg cgagaatact gttttaatat
     5521 ctgaagcctg acttccttct agtcctataa gtgcatgttc tatacggagc tttatactac
     5581 ccgttatgga aatatcttca aagcgcaaga gattatcgaa gttttcgtaa acagaaccag
     5641 cgagtgtatt ttcaatggga atcatcccgt aatcagccct tccatcgcga accgcttgga
     5701 aaacttctcg gaagcttttt tgtggaagcc tttcattgca ggcatcaaaa taatgaagaa
     5761 tggcttgctc agcataggca ccacgtgttc ctgaatacgc acaagaaaaa cttgttttag
     5821 ccccatcaga gcctgcaatc gcttcgcttg ttttgggctt agaccgaata cggctcactt
     5881 cttttcccac cacgggggat aaaacttcga tatcgcgcat tagtttttca aactgctcag
     5941 gatacagcga ctgtggtcca tcagacagtg ctttatcagg attttggtgt acttcaacaa
     6001 tcaccccgtc agcacccgct gcaacggctg caagcgcaag tggtggcacc ttatcgcgaa
     6061 tgcctaccgc atgacttggg tcaacaataa tcggtagatg cgtcatagag cgtaaaacag
     6121 ggatagctga aaggtcgagc gtgttcctcg ttgccttttc gtaagttctt atccctcgct
     6181 cacagagaat aacatcgttt gttcctgagg ataataaata ctcggcggcc atgagccact
     6241 cttcgatggt tgcagaaagg cctcgtttta agataacggg tttttctaat tcgcctactc
     6301 gcttgagtag ttcaaagttt tgcatatttc ttgcgccgat ttgatagaca tcgacgaggt
     6361 ctttcataac aggaatatgc tcgaccgaaa ctatttcagt tgctacagga agcccgtagg
     6421 catctcctgc ttcttttagg atttttaagc cttcttctcc cattccttga aaggcatagg
     6481 gactcgtgcg tggcttatag gcacctccac gcaataaaac agctccagca gaagagacgc
     6541 gttttgcaat ttccatcatt tgctcgcgac tttcaaccgc gcaaggcccc gcaatagcag
     6601 taacacgagt gccaccaatc ttgataattt gcccccaatt attggggatt tcgacaatcg
     6661 tattttcttt tttaaattca cgacttgcca tcttataggg cttggaaata ggaattacgc
     6721 gatctacccc aggaagtatt tccacttctc ttggatcgat ggacattttt ccaacagcgc
     6781 cgaagatagt ttcttcttct ccgcgaatct catttacctt aaagtgattt ctttcaagaa
     6841 atgattttaa gttttctcta tctcttaggg ggataccccg ttccaataca attaccatag
     6901 tagatccttt ttaggcttct aaattccaag aaacagttcg taatatatca ccaaaaacgc
     6961 cagcagctgt tacagccgcg ccagctccat aaccacgaat ggtaagtggt atggggttgt
     7021 atcgtgcggt gtgaaagaca aaggcatttt cacaatcttt tatcgggtag agcggatgat
     7081 caggaccaac ttcgagcatg ccaacactac attttccatc ttctatgaaa cctgccatac
     7141 gcaaaacctt attttctttt ttgagcgcag ccattttttc tgcaaaatag ctatcgagct
     7201 tgggtaagtt ttgaataaag gcatcgacgg agcctgtgtc atcaaagtct tcaggaaaaa
     7261 ctcggttgat aataacgtct tctatttcaa gctcatagcc agcctctcgc gcaatgataa
     7321 gcgcttttcg agcaacgtcc atgccaccga ggtcatctcg tgggtcaggc tcagtaaatt
     7381 ttttctcccg agcttctaaa accgcttggc taaaactggc tccctcatca agacgcccaa
     7441 agatatagga aagtgagcca gacataatgc cgtaaaaacc tgtaagactg tctccgcttt
     7501 taaataaatt ttgtagagta tctataatag gaagtcctgc ccccacattt gtctcgtata
     7561 aaaagcggcg atgttgtgta tttgcaacag ttcttaaaga cttataaaaa tccatactca
     7621 aggaatttgc ccgtttattt ggtgttgcta tatgcatacc tgctttgaaa atatctaagt
     7681 attgattggg taagtcatct gaagcggtac agtcaacaaa gatgggatta agcggttttg
     7741 ccgcttgcac aaagtccaaa actccttgta aatttgtttc cgttgttgaa gcctctaagt
     7801 cagagcgcca gttcgttaaa tctaaacctt gttcatccat catcatacta gaaaccgtag
     7861 caattgcgac aaccttaatg tctatttttt gagtgagcag ttcttctttt tgatctcgaa
     7921 tttgatcaag aagacaacca ccaatggcac ctgcaccgaa aacaaaaacc tcgatgcttt
     7981 gtgcttggtt gaagaaaaat aaatgtgcta ctttcacggc tatgtcgcca tattctcctg
     8041 atataacaac agatatcgag cgctccgatg agccttgggc gattgcaaga atgtttatat
     8101 ctcgtgaggc aagtgaatca aagaaggttc ccgcaacgcc gcgctttttc ttcattccgt
     8161 ccccaacaat cgatacaatg gcacagtttt cttgcacctc tataggattt ataagtcctt
     8221 ctgtaatttc catttcaaat tcgtcattta agacaaaacg cgttttttca gtatcttttt
     8281 gctttacaca aaaagtaatc gtatactcag aactcgattg ggtgataagc aagatagaaa
     8341 cctttgcgcg agacacagct gaaaaaatac gtgccgctat gccctgcttt ccgcgcattc
     8401 cagcgccaga aactgaaatc atggcggtgt tttttaagca agttatacca cgaaccgcgc
     8461 cacttgtcga ataatcaaac tcaaagggtc ccttccctat gcgagtacca ggagcttctg
     8521 ggttatagct atttaaactc caaacttcta tattttttga tgcgagcggg gtaagcgttt
     8581 ttgggtgtaa aacctttgag ccaaaaaaag ataattccat cgcttcttcg tatgacatat
     8641 tgtccacaac aatggcatcg tgaacaattt tggggtctgc ggtgtaaaca ccgtcaacat
     8701 ctgtccaaaa ttcaagcttg cgcgcttcca tcgcatgagc aacaatggca gctgaaaaat
     8761 cagaaccatt tctaccgagt aagcccattt gcacagggga gtttatatct tttttccatc
     8821 cacagataaa tccaggaaag agatagacag tgtttttgtc ttttttctct tcgcgaattt
     8881 ttgcaaaggc atcataggtt gcttgaaaat cagggtcgcc ttcaagtaaa tcacccgttg
     8941 caataatata atcccttgaa tcaaaaagaa aaaccttctg tccctttgat tcaaggatag
     9001 ccttcataat aggggcagaa aaacgctctc ctaaacccaa aatctttgca gtaactgagt
     9061 cagggcattc gccaaggatt tttattgcat tgagtagaag cgtatattcc ttacataagg
     9121 cgtcaacaac ggtaagtagt tcattataaa gattttcatt tgccattaaa tgtaaatctt
     9181 ttataatgtc tctatgtatg atttctatct tcattgctaa aagatggata tctggttttt
     9241 cttgtgttaa aaccgtatcg atactttctt gtaaaagatt tgttactcca gcaacagctg
     9301 aaacaacaag acttgtcgct tctttttctg agcgagacaa tacaatcgat gcggaattga
     9361 gcattctttt tgcgttggcc atagaagtgc caccaaactt taaacttacc attaatatcc
     9421 tcaaatataa cttataatta tgcaaaatct tagcaaaaat cgaaacaata ttccagtact
     9481 ttataaatca ttgttagttt actttttttt gcttgtgttt taaactaata tttactacta
     9541 tatatctatc ttttgtactg gagtaatatt tgaatgtagc aatcgccttt gacataagtg
     9601 ccctttctct ttttacagta atactattgc tctttatcaa taaaagaatt gcaccaacac
     9661 aaacaaatcg tgtttttatc ttatttgtgc tttgtctctt ttttacaagc ttcttttccc
     9721 tcgcctctct tttacacaaa agttatactt ttatctcttt tatacccttc gattttttta
     9781 caataacaaa ccatgcgcta aaaatttcta ttccactttt atttagttat tatatattta
     9841 cactcgttgc aagtcagaaa aaaacttctc gtagatttat aaaatataca ttgctggcac
     9901 ctctcatcta ttcgattttt gctctcactg catttatttt taacctgcaa gcctccatag
     9961 ctcaagcgtt ctatctgctt tacaccttta tttttctttt acaaaacgtc ttatttctaa
    10021 aaaaacatcg ctctattttt ccaaaacaaa aaacaatagt acttctatct tttgtagtac
    10081 tttttaccct ggtactcaat gcagaaatat acctagcttc tttttctatg ggaaatataa
    10141 tttttgcttt gagtattctt gtacttttat ttactacaca tagacaagaa gaaactattt
    10201 gcaatattag tttgttgtat aatcaaactg catttagttc aatatatgca aattattttg
    10261 aaaaaaaaat tccctttact atcgttacca ttgtgctcga tgatttattt tatttactaa
    10321 aaacgctcgg catacagcaa ataaatgcaa ttataaaaga aacagctttt ttcttaaacg
    10381 atatagcaaa aaatgccctc atttttcact ttaatgatgg caaattttgc gcaactttta
    10441 atgatacgag cgaagaagca tatcttactt ttattgaaaa aattaaggaa cgttttgcta
    10501 aagcatggca aattgattta atcgaaatta aaattttccc cagacttgct cttattgcat
    10561 gcccaaagga tgctgaatca attgaagatg ttatagaaat cctggagatt ctccaacacg
    10621 acgaaaaata taaaaaagaa ggcttactct atgcctcgca aatcgatacg agctatcgtt
    10681 taaaaacagc ttttttagat catacggtaa aaaatgcgct cgctgaaaat cgcatagatg
    10741 tctactatca agctattttt tctacggaaa aaaatcgcat tataggggct gaagccttgg
    10801 tgagaatgca ggatgaaaag ggtcgctaca tttcacccga ggactttatc cccatagctg
    10861 aaaaaaatgg cacgatacta cgcattggcg aatatgtcta tgaatcggtt tgtcgtttta
    10921 tcaatgaaac agatcttgcg ttttacggta tagaaaaaat ggatgtaaac ttatctgttg
    10981 tacaatgtat gcaggaaaac ttaagcgaac agtttttaat gattgctgat ttatacaaaa
    11041 taccttacga acatttaaat tttgaaatta cggaaacagc agcaatcttt tcaaaaaaaa
    11101 tatttgaaca aaatattcag cgctttgcag aagcaggaat agagctttct cttgacgact
    11161 atggttcagg ctattcaaat attaattatt taataaatct tcctttcaat atgattaaaa
    11221 ttgacaaaaa tattgtatgg aaggcttttt taagcgataa atcatatacc gccctctctt
    11281 ttactatttc tatgataaaa gctcttggaa tgacggtact tgcagaaggt gtggaaacct
    11341 acgagcaagc gcagcaattg acaaaactag gttgtaatta tttacagggt tattattttt
    11401 caaagcccgt accaaaggaa ttatttttat cacttcttat tcaaaatcaa acagagaatg
    11461 aaattttaga atttattgac tcgcggaatg caaaaacaca aacaaaaaaa atacttcatg
    11521 attctataaa agaattgcaa agtataaata aaaatacttc tcaaaaaagc gctgaaacag
    11581 aagactttga tgttcttgaa ttagaagaac tagaagaaat tgaagagctt aaagagattt
    11641 aaaaaacctt tatgcttttt tagaaaaact atatcgtatc ttaccatata cacgagcaat
    11701 aactgaaaca atggcaattc ccaaagctat cgcaccagcg gcacctaagg ttgtatagcc
    11761 gagttcgagc gctgtttcta cagagccact cacaaaggca cgcatggtaa aaaacatacc
    11821 tccacctgga acaagaggta agatgccggg aattaaaaac acggttgctg gattttttat
    11881 aataatagct aaaatttcag ataaaagtgc aacaaaaaag gcgcctaaaa aataggcgta
    11941 tagaatagaa ccaccgctga gtaaaatcca cttatagatt gtccaaccga tgcctcctag
    12001 aagcgctccc cataaaacat cgtatttgtt tgcattgaaa aaaagcgcaa aaactcccgt
    12061 cgccataaag gcagagaaaa aattccatag aaggcttgtg ttttgacttt gaaaaaaaag
    12121 gctcatagaa aaccaccaaa taaaagaggc ccagaaaccg caccaactga aagtccgata
    12181 gcaatcataa cagcttccac aaggcgtgca tttcctgaca ataaatcacc agcgattaga
    12241 tcacggattg cattaacgag ggcaagacca ggaactactt gcatgagtgt tgcagtaaga
    12301 ataatttctt gcgtaaatgc aagtggtata aaatctatca gttcaacaaa aatagaaaca
    12361 atcgctccag aaaaaagaga aatgataaaa ctattcagtt gtattttctc aagagctttt
    12421 aaaaaaagcc gtaagccaag ccctatacat aaagaaagga ctgcatcgat gagtcttccc
    12481 ccaaacataa aggagaaaaa aaagccactc aaggcaccca tgaggataat aaaccaggaa
    12541 ggatattctg cattcgtatc gattttactt aaaacaaagt ctatttgtcc tggctttgca
    12601 agtgattttc tttgtgcaag acggcggctc agttcattta cacgggatat tttttttaag
    12661 tttacttctc ggcgagttac tcgacgaaat gcggtatgga agctatcttt tgagtccgta
    12721 tacgaaactt gtacaacagt cggtgttaca aaggctgttg cggtttttgc tccaagagaa
    12781 attgcggtat gcgtcgccgt ttcttcggcc cggtaggttt caccaccgtt ttcgagaatc
    12841 atagaagcgg aacgaatact tatagctaaa acttctttat gctctaaacg cgactcattc
    12901 gttataaaaa ggtttgcatc tccctttttt tccaaagcta ggttctcctt tttttagata
    12961 taaaatgaaa gagggtaaac aacaaaaaaa caaagccggc aaaaacaacg gttaccgccc
    13021 cagaaggtaa atctaaggtc caactcaagg caagaccaga gagagaaaaa agaagtgaaa
    13081 aaatacttgc aaaaaccatc atcgacgata atgattttgc catgtaggat gcggtacctg
    13141 aaggcagtgt aagcatagcg ataaccataa cgagcccaac aaaggtttgc aagagcacta
    13201 cagatattgc gatgataata agcagaacga aaaaaacaag ctctgttttc acgccacgaa
    13261 cgcgagcaaa attttcatcg aaggaggagg agagaatttg cgggtaaaaa agccacgcga
    13321 aaaagaggac aattaaatca agtatagcta aaagtaataa atccattttt gtaatgagca
    13381 aaatatttcc aaagaggtaa ctcgaagggt cagtatagcc tggcgttttt gccataaata
    13441 aaacccctat actcataccg atagcccaaa ccgcatttat aaccgtgtct tctctttgct
    13501 ttgacttagt cgaaacaaaa aagataataa gagccgataa aatagcaaat aaaaaagcac
    13561 cagctaaggg agtaaaaaat gggatacgac cgcttacgga aaaaaagacc gccattccta
    13621 tgcctcccaa aacagcatgg gaaatagctc ctgctaagct tgcaatgcga cgaacagaaa
    13681 cgagggaacc gagaatgcca aaaaggattg cagataatat agaagcgatt accgcattta
    13741 aaagaatatc agtcatggtc gcaacatcct ctatcatcga ttatttcatt gtgatttacg
    13801 cgaactaaac ctgttgcact taaatctact tccgaaagtt ttgggctcgt tgtatgtttt
    13861 accaccgatc gaaagccccc cacctcatct ttttcccccg cacacaagac tacatcagtt
    13921 aaactggaaa caaactggga gtcatgcgtt acaaccagta tcgtgctctg cccttttaat
    13981 tttcgaagcg tttcgaacaa acgcttttcg ctttcgctgt ccatattcac cgtcggctca
    14041 tctaagacaa tgagctttgg ctgagaagcg agagcacgag caactaaaac acgtcttctt
    14101 tgcccaccag atagagcagc atagggacga tgagcaagat ttgacacttc gcttaaaacg
    14161 agggcttcat ctatcgcagc tttttcttct ttggatattt ttttccccag ccctttaaca
    14221 gttcccattt taacgacatc gtagaccgaa ataggaaaag aactatcgaa atccatatat
    14281 tgtggcacat aaccgatgag ttcttctctt tttttgcttg ttgaggggaa aagttcaaca
    14341 ctaccctttt gaggtttttc caagcctaag agcaatttta ataaggttgt ttttcctgca
    14401 ccatttgcac caacgagggc aacaaattca ttttcatgca catgaaaaga tatattttca
    14461 aaaacagaaa cagaaccgta tgcaaaagaa acatcgttaa aacgaaaggc aattgttttt
    14521 tgcccttcat cgatatcttg cttttctaaa aatggtgatt gtcttgaagc ttttttcatc
    14581 tataggcttc ttttatggtc ttcgccatag cgtataggtt ttgaatataa tcttgcgaaa
    14641 gtgggtcaag cataagcacc cgcgcccccg tctgttttgc tattttttct gcggcctgtt
    14701 ttgaaaactg tttttgcaca aagatagccc gagcattttc ttccttaact aaatctacca
    14761 atccagcaag ttgctttgca gttggctctt tcccgtcaac ttcaaaggct atttgtataa
    14821 gtccaaattc atcaaaaaaa taaccaaagg aaggatgaaa tacaaaaact attttccctt
    14881 gtagatcctg taaatcgaca gcaatctcat taaaggtttt gtcgattaaa aaaataagag
    14941 aatcaaagtt ttttttataa aagttcgtat tttctggatc gatcgcaatg aggctaagat
    15001 agatttctcg acttataagt tttgcatttg tgcgaccaag ccaggtatgt ttatcaactc
    15061 tagaacttaa aactcgctct tgagaatgca cagaaatgtc tcttgaatca attacggcgt
    15121 catcatcgtg ctggtggcta tcttgctccc ctggagaaaa atttcgatac tgcaaggttt
    15181 ttgtaatatc tataatagaa agatttggga tttgcttttg tatttttgga acaaagcctt
    15241 cttcaaaatc caagcccgat aaaatccacg catcagcctt tgtcaatttt gaaatttgac
    15301 ttggctttgg ttcataggca tgaggacttt gcccttctcc tactaaagtg agaatatcaa
    15361 ccctatcttt tgcaatttcc tgcaaaaaaa atgcttcagg taaaatgctt acggcgacaa
    15421 gcggttttgt tttccccccc gtatcgcgag aaccaaaggc aaaaagattt tgcagagaaa
    15481 ataaaaatag aacagtcatt aaaaataata ttgttttttt catatagcta tattattcaa
    15541 aaaaaagcct aaatgacaag ggagagagta acttactcac ccggatagac aattccccat
    15601 tttgcacgca aagtatccat cacttccatc actttgatta tttctgaatg cggcatttct
    15661 ggacactcga gaagcccttg atcgattgct cttttacaag cgcgcacttg gtattcaaag
    15721 ccagtaattt gcttaggagg gaagtactgc tcgataagcc gatattcact gtcatagacc
    15781 tttattccct cacaattatt gatattttca aactcaataa atccctttgt tccaaataaa
    15841 agcccacgcc tatcacttgt gcacgagata gatgattgta agcttgcaag acgtccatct
    15901 tcataaacaa ggcttataga atttgtttca tcaacacctg tttttgtttt aatacatgtg
    15961 ccatcaacag aaagcggatg cccaaaagcc atcatagcaa aatttattgt ataaacacct
    16021 acgtcaagaa gtgccccacc agcaagcgca agatcaacaa ccctttgttt atgcgtaact
    16081 ggatatgcaa gatttgcagt aagtgcatat atttcaccga ctggcttcct ttccaagatt
    16141 tcatctaagg tttttctcat tggcatatac cgtgtccaga tagcttccgc aacaaggagt
    16201 tttttttctt tagcaagagc caaaatttcg cgcgcttgtt tcgcattcat tgtaaaagat
    16261 ttttcacata aaatatgttt cccatactct aagcacagtt tcatatgttc aaagtgatgc
    16321 gaatgaggag ttgcaatata gactaagtcg atatccttat cttctaacat tgtttgatat
    16381 gagccataaa tctgtggaat ttcgtatttt tttgcaaagt tctctgctct atccttatct
    16441 cgtgatgcta ttgcgtatga ttttgcatca tccatcgcat taagcgtttc tgccatcttt
    16501 gttgctattc ttcccgcacc taaaattcct actttcatca taatacatcc ttatctagaa
    16561 tatttcccct aacattacta tacgctataa aagcattttt agcaatttta ttttattagt
    16621 taaacannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaaa aacaaattga
    16741 tataaaagga tactgtgtct caaaagaagg aagaaaaatt cccatctctg tttcaaccgc
    16801 acttttaaaa gacaagaggg gaagaatttt aggaggagct gaaacctttc gcgatttaag
    16861 cgaaatagaa aacttgaaaa aagagcttaa acgagcttct cttggaaagc ttgaaagtaa
    16921 aaatgcaagt atgcaaaaga ttcaagatat gcttccatcc cttgccgaaa gtcttttgcc
    16981 tgttttaatt caaggagaaa cgggcaccgg aaaagaaatc gttgcaaaaa gtcttcacca
    17041 actgagccct ctcaaagatg gaccctttgt tgcaataaat tgctcggcct ttccggagag
    17101 cttgcttgag tctgaactct ttggctataa aaaaggcgct ttcactggag ccgataaaga
    17161 caagcctggt cgttttgccc ttgccgaaaa tgggactctt tttctcgatg aaataggaga
    17221 tatttcgctc aatctccaag taaaactttt acgtgttctt caagaaggag agtatgaagt
    17281 tattggagga agaagtccca taaaaaccaa tgcacgaatt attagcgcca caaatagaaa
    17341 actagaagag ctcattgaag ctaaaaaatt tcgcatggat ttttattata gaatcaatgt
    17401 tatcggttta gaactgccgc ctttaaaaga acgaacagaa gatattgtcg actttgcaga
    17461 attgttttta gacggcgctt tttttaggaa aaatcccacg agcgatgaaa acccctattt
    17521 ttttagtgcc gaagttttaa ggtattttta taactacccc tggccaggaa atattcgaga
    17581 acttgaaaat actgttgaaa gaatgcttgc tcttgcgagg tcctatgaat tggacaaaaa
    17641 acttttaccg gaatccttct ttcaagaaaa gaaaaagcat gaaggaaaac aaggtgacga
    17701 tttaacgagt attaaaaaaa tacaagaaaa agcagaaaaa aaaatgctca taaaatgtct
    17761 tgaaaaaaat aactggcata agggaaaaac agcaagcgac ttggaaatag atacatcgac
    17821 gctttggcgt aaactaaaaa aatacggcat agaaagagcg actatataaa aaccagcctt
    17881 ttcttcctag cctttcaatt ttttttacgc aagctctttt ctctgtagat ttttggcaat
    17941 aaggcgagca gcttttttta taaaaagggc acaagggcga gaccgataat aagcctcatc
    18001 tcttacttga ggattttttt gcggatttat atcgtattct tctaaaagat ttttacagat
    18061 agtagtccca aattcttttt caaagtcttc gattgccgag cgaacgagtt cataaaagac
    18121 tgttttttta tcattatcat ttggatcata gtcgccgtgg gcgagaccga gaatatatac
    18181 catagcagaa attgcgccac atgtttctcg cctacccgaa aaaccgccgc caaaacctgc
    18241 catcgctttt tgtacatctg ttgtttttaa gtcaaaaaat tcggcaaagg cgagggctgt
    18301 tgcttgagca caattatagc cagcataaaa aaaagaagca gctttttctg gatagtcgtc
    18361 tgtttttact aagttcataa aaagagttta tactattttc cctatcaata atagtagcaa
    18421 gataaaaaaa tcaaatattg cacaaatgca atactgcaat gcacaaatgc aataatcata
    18481 ttctaaagga gcctaaaagt ctcaaaaaga cctcctaaac ttggcacagt aattgctttg
    18541 ttatagctat gaaaaaagca atatcaatat ggaacaatcg aatagctccc gtctttgatg
    18601 aatcacaaga atgtatcatc atagaggaag actattcaaa aagcgaagtg caaaaacttc
    18661 cgctcggttt ggaggataaa attcaatttt tgctagacaa tgaagtgggc accctcatat
    18721 gcggtgcaat atctcggttt gccgaaaatc aactccttca aaacaatatc gatgtctcgc
    18781 cctttgtcgc aggttcagta agcgaagtta tttcagcctt agaagaggga aagctctata
    18841 caaaaaaatt ttcaatgccc ggttgtcctc gcccctttgt tcgccgtggt tttggtatgg
    18901 gaagaggcat gggctgcgga gctggtcgcg gtcctggtgc ggtttttggg cacggacgag
    18961 gctttggcat ggggatgggc tctggcaggc aggaagctgg cagagggctt ggcttaggct
    19021 acggtcttgg tccaggaaga gggcttggct taggctacgg cagaggcttt agacaaggag
    19081 cattttatac tgaggatgaa aatatcaatt acggacaaaa cgacagtcct caagaatttt
    19141 caaaccaaag aatacaagga gatagaatga agatagcaat ttctgcaacc ggaaaagact
    19201 taaacgcaaa ggtagaccta cgatttggtc gggcacaaaa ctttattatt tacaatacag
    19261 aagacaaaag ttttgtagct atagataata tacaaaacct caatgcccct cagggagctg
    19321 gtatacaaag tgcccaacac atagtcgact ctggtgcgag cgttcttata acaggaaata
    19381 ctggaccaaa ggcttttcgc gttctcgaag cgggaaatat cgaagtgttt ttaagtgaag
    19441 ctataagcgt tcaagaagct atcgagcgat acatgagtgg ctcattagaa aaaacaaacc
    19501 aggcaaatgt tgaagggcat tggtagaatc gatgaaaaat gttttacaaa tagccatcgc
    19561 ttctggaaag ggaggaacag gaaaaacaag tcttgctgtc gctcttgcaa aaagccttac
    19621 tctctcaaaa gagaagactc ttaaacaaaa agaaatctat cttgcagact gcgatgtaga
    19681 agagccaaac gccaagcttt tttttagtgc atatttttct gaagaaaaca aagaaacaaa
    19741 aaaatatccc acgagtctag aaagagtcga ggtccccatt ccgcaaatta atcatgagct
    19801 ttgtacgctt tgtggaaaat gcgtagagtt ttgccagttc aatgcgctcg ctcgtgtagg
    19861 aagagttttg gtctttgatg aactctgcca ttcttgcgga ggctgtacac tcgtctgccc
    19921 cacacaagcc ataagtgaag tgccacatag tataggaagc aaaacccaaa aaacacttcc
    19981 tcacaaagaa aaagatattc actttataga aggaaaactc gatgttggaa aagcactctc
    20041 tccgcccatt attcgaaatg taaaaaaaac acttgctgaa aaaacaaaag ataaaaacgc
    20101 aattattata ctcgactcgc cccctggtgc ttcttgtccc atgacaacaa caatcgatga
    20161 agttgacttt gtcatcctcg ttactgaacc aactcccttt ggcttacatg acttaaaaat
    20221 tgctgttgaa acagttcgac aagttggcta tccctttgga gttgtcataa accgaagcga
    20281 tgctggagat agtcgtgtta ttgaatactg cgaaagcgaa aaaattcccg tcctccttga
    20341 aataaaagaa gacatggata tcgcaaaagc ctattcaata ggaaaaagta ttgttgaaac
    20401 aaagagtgaa tacattgagc tttttattgg cttgtacaaa aaaatagaag agcttgttga
    20461 gaaaaaaaat atctttcaaa aacctgctat tacaacagga gtaaaattat gagtgaaaaa
    20521 aaactaaaag aaattgttgt tatatcaggt aaggggggaa caggaaaaac aagcttcagt
    20581 cttgccctcc ttgatgcact gtctcaaaat aaaaaaatcg ttgcggctga ctgcgatgtc
    20641 gatgctgcag atatgcatct cgtcctctca ccagagatac aaaaaagcga agtatttata
    20701 agtggcaatt ttgcacacat acaacaagaa aaatgttccc gtgcaaaggg ctcttcttgt
    20761 tccgtttgct acgacctttg tcgctttgat tctataaaaa aaatcgagag ttcagaaatt
    20821 ccagaggcaa aatctgcaag taatccaatc cgtttttcta ttggagaaaa tgactgtgaa
    20881 ggctgtatgc tttgtgtcga attttgtcct gaaaaagcaa ttgtatttcc agagcgaaaa
    20941 tgcggaaagc tctttgtttc taaaacacgt tttgccccac ttgttcatgt tgccctcgat
    21001 attggttctg aaaattctgg aaaacttgtt acagaggtgc gaaaaaaagc gaaaagcctt
    21061 gccgaagaaa ctaattctga ttatcttata gttgatggaa gcccaggaat aggctgccct
    21121 gtgattgcat cgattacagg agctgatcaa gtgcttattg ttactgagcc gacgctttct
    21181 ggaatgagcg acttaaaacg agttgctggc ttggtaaagc attttaagat tccacatgcc
    21241 gtcattgtaa acaaggcgac agtaaatctt gatataagcc aagagataga aagctggtgt
    21301 tcaaaaaata atatttccta tttgggtgaa attcactata ataaaaatgt aacgcttgcg
    21361 cagttacaag aaaaaacaat tattgagtat gaaaaagaag aggtggaaaa accctttact
    21421 tcttttatac aaaaaataat aaaggatatt ctatgataga tttagtctta atgcccactg
    21481 ttaaaaaaaa atacgtcgtt atgtcaggta agggcggagt tgggaaaagt actgtttcgg
    21541 taaaccttgc cgtaagcctt gccctccaag gaaagaaagt tggtctcctc gatgtagaca
    21601 ttcacggccc aagtattccc acaatgctcg gcttagctgg tacgaagctc accggaagcg
    21661 aagaaagtat cgaccctgtc ttagttgacg gcataccaaa ccttaaggta atgtccgttg
    21721 gttttttact ggagtcagaa gatgcaccag ttatttggcg cggtccgatg aagaactcca
    21781 ttatcaaaca atttttaaaa gatgttacat ggggtgaact cgactatctc atcatcgatt
    21841 gtccccccgg cacaggggat gaacctctgt cggtagtaca actcttggga aaggctgacg
    21901 gtacaattat cgttacaacg ccgcaagatg tcgcatcggt tgatgtgagc aagagcataa
    21961 aatttgctga acaagttaag ataccggtaa caggaattgt cgaaaatatg agcggctttg
    22021 tttgcccaca ttgcaatgaa gtaagctata tttttaaaga aggtggcgga aaaaaacttg
    22081 ctgaagaata caaagtcccc ttcctcggtg caatcccaat cgaccctgcc cttggtgaag
    22141 caggcgatag cggcaagccc tatgtagcgc aatttaaaga ttcgattatt agcagaactt
    22201 atgaagagat gacaaaggtt ttataaaaat aaaaaatacg agaggagtag acataatgaa
    22261 aatagcaatt cccgtagcaa acggaaaact caacatgcac tttggtcact gcgaaggctt
    22321 tgatcttttt gaggttgata caaaaacaaa aactcttgga gaaaaatcat acatccaagc
    22381 gccaccacac gaaccgggct tactaccgcg ctttttaaac gagcagggcg taagcgatat
    22441 tattgcggga ggtatgggag caagagccca agagcttttt gtcgaaaatg gaattaaggt
    22501 cctcgtcggt gctccatgtg aagagagtgc aaagctcgcg caagattatc ttgatggaaa
    22561 actccgtcct ggttcaaatg tctgcgatca ctaaatcgct accgatacag agatgataat
    22621 tactatcctc gcagacaatc gcttctgtaa cagaggcggt tctgcgaata aaaaccaaga
    22681 ccttttaaca gagcatggct tttcttgtct cctagaaacg aatgacggac aaaccatgct
    22741 ctttgatctt gggcaaaagg tgtttataca taatgccaaa gtcttaggaa aaaatctcaa
    22801 agaacttgat gctcttattc taagccatgg acactacgac catagcgatg gactttcagc
    22861 attttttaca gaaaacacaa agacaaaact ctttgcatca aaacatattt tcacagatca
    22921 ctttagtaaa aaaacaggga cggcgcgatt tatcggctta aacgaaaaaa ataaaaacat
    22981 catagaaaaa ctccccaaaa cacaaaaaat acttttttca aaacaaagcc tcgtctgcaa
    23041 atataaatcc ggaacaaaca aacaccgcat agaagtacac ggagcgatta aacaaaaaca
    23101 tccactcgaa aaaccctctc cccttctttt tgctgatgca gactgtacag aaagcgatac
    23161 aatgctcgac gaaacaattc ttgcgattga aacagataaa ggtttgtttt taatcacagg
    23221 ttgcggacat gcaggtttta tcaatatttg cgaaacaata cgaaagcgaa cgagaaaaaa
    23281 aatacttgct gttctcgggg gctttcacct tgaaggcgtt tcaaaagagc gcttaaaagc
    23341 aactcgagat tatataaaaa aaacgggcat agaaaaaata tatccctgtc attgtaccgg
    23401 cgaaaaagaa atagaatggc ttgaaaaaga actacccggt attgcacaaa aagtttgcgt
    23461 aggagatcta atcaagctcg attcatttga agaaaactaa tacaaaaaaa aacgcacaaa
    23521 tgaaccctcc tttctagagc tatacaagag acccgcttca tgttattctg tatcaatgcg
    23581 aataaaacaa gaatcatctg cacataggtg gaaatttttt agagcaggaa atgtcgcaca
    23641 agttgtctta gaaacagacg atgacttacg acatttagca gagctcgatc aaaaactctg
    23701 gatggtactt tccatgcctt gcaaaggcgt acagctcagt gaaaaaacct tgcggctttt
    23761 agacagcgac ggcgacggct ctattcgccc gcctgaaatt ctagaagcgg tttcatggat
    23821 ttgctccact ctaaaaaaac cttctgtcat ctttgaagaa ggctcagatc tagaactaga
    23881 aaatattcaa gacgcagagc tacaagcgag cgcagccttt ttgctcaagg ccctaaaaaa
    23941 agaagacgag acatctatct cctacgaaga tgcccacaaa cgaagtgaag cctttgcaaa
    24001 ccttcgtttt aatggcgacg gaattattga acttgagata ataaaagatg cgctcttaaa
    24061 agaggctgct tcaaaaatat atgcagcgca aaaagaaagt cctgctgaaa gtagcgaaga
    24121 agaaattccg tctataaata gaaacatgat tgaaagcttt tctgaaaatg taaaaaacta
    24181 ttttagttgg cttgaaaact tagaaagagc ttttgaatct atccaattga atcagacaat
    24241 agaagaaaaa aataaaaacc tcgaagtcta tatagctacg atacaagcct ttattgctgt
    24301 aaaagataaa atcgatgatt attttttacg ttgccaattt gtagaatttg atagtcgaac
    24361 agaatctatt ttgcaaggca atgaaaacga actagaacag ataagcaaaa aacttttatc
    24421 ccaaaacgat gagatactcg aaacctttcc tatctccctt gtatcaaata cagaaagact
    24481 ttctctgcat gaaggtttaa atcctctttg gaaagaacgg gtgaaaaatt ttgtagaaag
    24541 ggtctatcat aaaatcccct tgagcgataa aaaagataca gacaagtttg aaacgctgga
    24601 taaaaaaaca tgggaaaaaa tctgcgaaac ttttaatccc atagtcgcca tctatgaaca
    24661 aaaaccacaa acaaatcttt cagaactaag gagcgaagaa ttaaaaaatc tagaagacgc
    24721 actgccagca cttttagctc ttgtaaaaaa agatgaggac tatgcaaaag aacgatgcct
    24781 cgtagacagc ctagcaaaac tcttacgcta tagacgagat ttgggaattg tcttaaaaaa
    24841 ttttattagc ctcgagcatt tttatacaaa tgcaaattct gtctttcaag caggaagtct
    24901 ctacatagat ggtcgaacct gccatctctg catcgatata gtagatgatg aaaaacatgc
    24961 aagcctcgct gccctctcgg gtgcctatct agtctattgt gatatttttc gcctagacca
    25021 agctccaaaa aaaattgttg caatgcttag cgcaggcgac aatgagaaca tgattgtggg
    25081 aagaaatgca atcttctatg atagaaacgg actcgactgg caggcaaagg tgcgccgtat
    25141 tatttccgct cccatcagcg taggacaagc tttttggcaa ccctataaaa aactctatcg
    25201 aagcattgaa gaccaaatta taaaacgaac acaaacaagt gaagatgcaa ttttcacaaa
    25261 aataaaccag ggagaaaatc tcctacaaaa caacacagta caaactaagg aaaacacaaa
    25321 gacgcctaaa aaactcgacc tcggagccat cgcccttatt gggacagcaa tcggtggtat
    25381 aagcgccctc gtctcagctt tttttcaaag cctctttggc ttaggactct ggctcccatt
    25441 gggcatcgca gccatcatac ttttaatatc cggcccctca atgatacttg cctctatgaa
    25501 actccgaaaa agaaacctcg gtcccatcct cgatgcaaat aattgggcaa taaatagcat
    25561 ggcacgcatc aacatcccct tcggctcgag tctcaccgag ctctcctccc tagcccctgg
    25621 aagcttggta ggcagcaagg acccctttaa gggaaaaagc ccaaaaccaa aaataattct
    25681 aggtcttgtt atagtgatag caatcgctgc aatagtttat tttttcccaa gtattctggg
    25741 ttttgtaaaa aaactacgtt atttctaaca tccgtgttag aaataacttc gacagtttcg
    25801 gatacccgaa actgtcatac ttannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25921 nnntctattg gcttgcctgc tggaaccatg ggaaaaacca tttcgtcgat gtccatgccg
    25981 cagtcaatga ggcagggttt tttctcgctt accgcttttg caaaacagtc gacgaattct
    26041 ttttcatctg ctgcgcgata gccacgaata tcaaaggctt ttgcaagttt tacaaagtct
    26101 ggtccgcggt cgagggttgt cgatgaatag cgttttccgt aaaacatttt ttgccattgg
    26161 cgaaccatac caagggttcc gttatttgca attataataa taacaggaat ttgataatgc
    26221 gaaattgttg caagctcgtt taagttcatg cgaaatgagc catctccagc gatgtgtaca
    26281 acgcgttgtt ttggttgtgc tatttgtgcg ccaagtgctg ctccagtgcc atagcccatt
    26341 gtgccaagtc caccagatgt tacaaaggtt tttggggtaa caaatggata aaactgtgca
    26401 acccacattt gatgttgtcc tacctctgtt gtaataatag tttcatcgcc aagtacttcg
    26461 tttatttttt ccataacgaa ttttggatgg aggcgcgttt ttttagagta cattgcagga
    26521 atatgctttt taagttcttc ggtttcaata ttccactcat cctttgcacg cggtttcaca
    26581 agttcggtta attctttaag tataatattt atatcccctg taagagatgc ggtggttgga
    26641 atgtttttat ttatttctcc tcgatctata tctatatgca gtacctttgc ttcgcgggca
    26701 aatttatttg ggtcagatat aacgcggtca gagaagcgag cgccaatggc aacaaagaca
    26761 tcgcatttat ttgcaagggc gttcgaagcc tttgtgccat gcattccgat catgccggta
    26821 cttaactcat gtttttgaga gaaggctgtt tttcccatga gtgttaccga cacgggtgca
    26881 tttaattttt ccgcaagggc tttaaatgct tcggttgctt cagcaatgac aactcctcct
    26941 ccaacaaaga aaaagggttt ttttgcggag cttaaaatag cagcaaagtc ctccaagtca
    27001 tctttagttg gaagtggcgt tttttgatta cttcttttta ccgcatcact ggttgcaata
    27061 tttggatcta tcttttttag aatataatca acttcggctg ctgttacgtc ttttgggata
    27121 tctactaaaa caggaccagg tctaccgctg cgggctattt caaatgcttc acgaatcgta
    27181 tcagcgagtt ttgtaatatc tcgaacaatg taattgtgtt ttgtaatagg cattgtaata
    27241 ccggcaatat cgacttcttg aaagctatcc ttacctagga ggcttgttgg gacattgcaa
    27301 gtaattgcaa caaggggaac ggaatccatg taggctgttg caatacctgt tacaaggttt
    27361 gtagcaccgg ggccacttgt tgccataaca acgccaacct taccgctcac tcgagcatag
    27421 ccatcagctg catggcaagc accttgttca tgggcggtga ggatgtggcg aattttatca
    27481 gaacttttat acaactcatc atagatattt aaaatggccc caccgggata cccaaagacg
    27541 gtatcaacgc cttgctcaag taagcattga ataacaactt gtgctcctgt cagtttttgt
    27601 atctcttgtg tttctgtttt tgtctggctt gttgtgcgag tctttgtcgc tgtcattatt
    27661 tttgtttcca tgtcttactc caatattgca ccttgatctg cactcgaaac caattttgcg
    27721 tatcgagcaa gatagccttt ttttattttg ggctcaaggc attgccaaga ttgttttctt
    27781 gtttctaatt cttcatcgct tacatttagc ttgatactat atgcgtttat atcaattgaa
    27841 atactgtcgc catcttgcac aagggcgatt ggtccaccga ctgcagcctc aggggagcaa
    27901 tgtccaatgg atgctccacg agtagcgcca gaaaaacgac catcagtaat aagggcaact
    27961 tctttatcca taccgcgacc agcgagggca gccgttaccg agagcatctc tctcatacct
    28021 ggtccaccac gtggtccctc ataacgtata acaattacct ctccgctttt tattgtgcct
    28081 ttttgaattg cttcaaaggc ttcttcttcg ctattaaata cgcgagcagg tcccgtatgc
    28141 ttcatcatct ctggtgcaac tgcagaacgc ttaaccacgc aaccattcgg cgcaaggttt
    28201 ccaaaaagaa cagcgatccc cccagtttgc gaataaggat tttctatggg acgtaaaatt
    28261 gttgggtcag tattttccgc ccgtgtaata ttttctgcaa ttgttcgacc agttacagtc
    28321 ataacagagc tatctatcaa atttttttta tcaagctctt taagaagggc ttgtatacca
    28381 ccggcaaaat ataaatcttc gataaaggca tttcctgcag gtgcaagttt acataggttt
    28441 ggagttcgct ctgaaatttc atttatcttt tttaaatcaa ttttaatatc tgcctcatgt
    28501 gcaattgctg gaaggtgtaa aatagtattt gtgctacaac cgagtgccat atctgcagca
    28561 agagcattat tgaatgaagc ttcattcata atttttcgag ctgtgatatt tttttcaatc
    28621 attgtcatta ccgccattcc agcttttttt gcaagggcaa tacggtcggc atagacggca
    28681 ggaatggttc catttcctgg taagcccatt cccaaaactt ctgtaacgca gttcattgag
    28741 tttgcggtaa acattccaga gcatgagccg caggttgggc aagcggagca ttccatttcg
    28801 tccagttcgc ttgcttccat cttgcctgct gccactgcgc caactgcttc aaacatatcg
    28861 gtgagggaaa gccgtttccc cttgtggcgt ccggcgagca tggggccccc agaaacaaag
    28921 acggtgggga ggtccatgcg agctgcggcc atgagcatgc cgggtacaac cttgtcgcaa
    28981 ttgggcacca tgacaagtgc atcgaaggcg tgcgccttgg aaacagcttc gattgaatca
    29041 gctattagtt cccgtgttac caaagaatat ttcataccct cgtggttcat cgcaatacca
    29101 tcgcaaacac ctatggcagg aaattccatt ggtgttcctc ctgccatacg cacgcctgct
    29161 ttaacagctt cgacaactgt atcgaggtga atatgtccag gaataatttc attcttggaa
    29221 ttgactatgc cgactattgg tcgtgccaac tcttcatcag tatatcccat tgctcgaaag
    29281 agtgagcgat gtggagatct ctcatatcca gtttttgcat tatcgctttt catatacacc
    29341 tcacaaatta tatttctatt tttatcttaa taattcttta acaactaaat cgcccatctt
    29401 tgttgtaccg acaagttttt ccccaagtgc ttttcgtttt tcatcacttc ctgctatatc
    29461 gcctgttcgc cagcctgcat ctaaaattgc attgattgca ttttctattg caagcgcttc
    29521 tgcttcccaa ccaaaggaaa agcggaacat catcgcaagg gacaagaagg ttgccaaggg
    29581 gtttgcaata tcttttcctg caatgtcggg agctgagccg tgaattggtt catacaaacc
    29641 aggaccagaa tctccaatcg atgcagaagc gagcataccg atagagccag tgatttgact
    29701 tgcttcatca gataagatat cgccaaacat attgcttgta acaatgacgt caaactgatt
    29761 tggattacgc acgagttgca ttgctgcatt atcaacatac ataaagtcca ggctaacatc
    29821 agcatagtct tttttcattt catgcgcaac ttctctccag agcctgcttg actctagaat
    29881 atttgccttg tcaacaagac aaagtttttt atttcttttt tgtgcgcttt caaaaccaac
    29941 ttttaaaatg cgctctattt ccatcttgct atatgtttca gtgtcgtagg ctcgctcgcc
    30001 gtcttcactt ctcccgcgct ctccaaaata aataccgcca gtaagttctc gaacgattaa
    30061 aatatcaagc ccatctttta cgatttcatt tttcaaaggg caggcatcgc tcaattgttt
    30121 aaacaataca gcagggcgaa gattagcata caccttcatg ccgcttcgta gtcccatgag
    30181 tgcttgctct ggtcgttggc ttcccggtaa gctatcccat tttggaccgc caactgcacc
    30241 gagtaaaacc gcatcacttg caagagctgc ctctaagctt tcttttggaa gtggcccacc
    30301 agtcgcatcg attgcagctc caccagcgag cacttctaca tagtcaaact cacgtgagaa
    30361 tttttttgca atggcgtcca aaactttttt tgcctcacgt gtaatatctg gaccaattcc
    30421 gtccccagga ataagcgcta tttgtttttg catacatcac ccaccttttg attgagttgt
    30481 tttatgagac catccgcttt aattatttct tgcataaagg gaggaaaggg agctgcttga
    30541 aagcgtttcc cactcgtgtt atttataatg atacctgaat caaaatccac cgcaactcta
    30601 tctccagcct ttatctccgc cgctgcttca gggcattcaa gaatcggcag tccaatattt
    30661 attgcgtttc tatagaagat acgtgcaaag cttgcagcta tcacacaaga aatacctgcg
    30721 gttttaattg ccaagggtgc gtgttctcga cttgaaccgc aaccaaagtt tttatctgca
    30781 accataatgt cgccagcctc tactgttttt gcaaagtcag cgtcaatatc tatcatgcaa
    30841 taagaggcaa gctcttttgc atccgatgta tttaaatagc gtgctggaat aataacgtct
    30901 gtgtcgacgt tgtcgccata cttaaaaacc tttccttctg ctttcatatt atttcctttt
    30961 tgtaaaaagt tatttttcgt ttttttataa aaaaatctta cctacccatc aaatcttttg
    31021 ggtcggtgat tactcccata accgcactcg ccgcagcaac tgcaggattt gacaaataca
    31081 cttcactttc tggatgcccc attctgccga caaagtttct atttgttgta gcaagtgctc
    31141 tctcgccctt tgccaaaata cccatatgtc cacctaagca gggtccacag gtcggagttg
    31201 aaaaggcaac atttgcctca acaaaaattt ctgccaagcc ttctcgtacc gcttgcaaat
    31261 aaattttttg tgttgcagga aaaataatac agcgaatatc tcgatgtacc tttcttcctt
    31321 tgagaataga tgcggcaact cgtaaatctt cgatgcgtcc atttgtacaa gagccaataa
    31381 caacctgatc tatttttaag ccctttgctt catcaatcga ttttgtattt gatggaagat
    31441 gcgggaaggc aacagttggt tcaattgttg caagatctat ttctataact cgctcatatt
    31501 ctgcatcggg gtcagcttcg taaattgtcc agtctttttt tccatgctct tttagatagg
    31561 caagcgtaac atcgtctacg gggaagatgc cgttttttgc cccagcttca attgccatat
    31621 ttgcaatcgt aaatcgacca tcgatatcta tagaagcaat tccatctcca acaaattcca
    31681 tcgattgata caaggcgccg tcaactccaa tcataccgat gatatgcagt ataagatctt
    31741 tgccgcttgc ccagccagag agtttatttt tcaagacaaa cttaattgca gagggaactt
    31801 taaaccaagc ctttccgctt gccataccag ctgccatatc ggtagaaccg atgcccgttg
    31861 aaaaagcacc gagcgccccg taggtacagg tatgactgtc tgctccaatt acacagtcgc
    31921 ctgcaacaac caagcctttt tcaggaagaa gcgcatgttc tattcccatg caacctactt
    31981 caaaataatt ttcaatctca tgtgctcgag caaactcacg aacttttttc gcctgctctg
    32041 ctgccttaat atctttgttc ggcgtaaagt ggtcaggcac aattgcaatc ttctttgtat
    32101 caaagacttt tttacaacca attttatcaa actcgttaat tgcaactgga gtcgtcacat
    32161 cgtttcccaa aactaaatct aagtttgcct cgattaaatc tcccgccttt accgattcta
    32221 gccctgcatg tgcggctaaa attttttgtg tcattgtcat tcccatcgta tttccctttc
    32281 tcgccagata gcgagttata aatatgtttt aaattttctt tcaatatcca ttatcaattt
    32341 atattcaaaa gaatctacca aggccttcca tgaggcttcg atcaagtcag cagaaacgcc
    32401 aaccgttgtc catgtttttt ccccgtctgt tgaatcaatt aaaacgcgca cctttgcagc
    32461 cgttgccgat tgggaatcca aaacacgcac cttaaaatcg gtgagtcgaa tatcttttac
    32521 cgagggatag aatttttcta agcccttacg taaggcagag tcgagcgcat gcactggacc
    32581 aaggccttca ccagcagtaa tcgatttttc atcttctacg ccaattttca cttgtgcaaa
    32641 ggcatttgaa ggaaaacctt cgaccgcttc ttccacaata accttataat aatctaagtc
    32701 aaaaaaaggt ttgtacttgc ccatgttttt tctcataagc aattcaaagc ttccctccgc
    32761 accttcaaac tgatagcctt cgtgttcaag ctcttttacc cgctcaacaa tttttgcggt
    32821 tataggacta tccttttgaa ttgaagaatc aaactttttc atcttttcaa tcacgaggga
    32881 acgccccgca acttcactca tcaaaaagcg tctttcgtta ccaaccatct caggaattat
    32941 atgctcataa gcagtcggat tttttgtaac tgcatcgata tgcattcctg ccttgtgtga
    33001 aaaggcattt gaaccaacaa agggcatacc attgtagagg gcaatatttg ctatctcgct
    33061 cacgcgatga caaatcgatg taagattttc tatacagcct tcaggtagac agggcatttc
    33121 catcttcaat tctaaatttg caataatcgt cgaaaggttt gcattgcctg ttcgctcacc
    33181 aaagcctagc aaggttccct gcacatgacg agcgcccgct tttaccgcca tgagagagct
    33241 cgcaaccgcc atgcccgcat cattgtgcaa gtgcacccca attgtacagc ccgtttcttt
    33301 caccgcctct tctgtgatct ttgccaagtc atcgggaagc attcccccat tcgtgtcgca
    33361 aagcacaagg gtttttgccc cagctttttt tgcaacttgc aaagttttaa gcgcatactc
    33421 agggtttgcc ttatagccat cataataatg ctctgcatca taaataactg ttttaccttg
    33481 ctcgacaaaa aatgcaatcg tatcttctat catatcgaga ttttcttgta agcttgcttt
    33541 taaaatttct gtaacttgaa aatcccaggt ttttccaaag atacaaatcg tgtcggttcc
    33601 tgcggttaat aaactttgta cattgacatc ttcagcaaca gctaaacctt ttcgccgtgt
    33661 tgagccaaag gcgacaagct ttgcattttt caattttatt ttttttgcct cagaaaaaaa
    33721 ctccaagtcc tttgggttcg aacctggatt tcctgcctct ataaaagcca ctcctagttc
    33781 atcgagcgcc tttacaatcg acagcttatc ttgtacagaa aaacttatac cctcaccctg
    33841 cgcaccatca cgtaaggttg aatcaaatat ttcaactaat ttattcacca ttcctccttg
    33901 ccttttcttg ctcgagttca aagaccatag aattgattgc aaataaataa gcctttatga
    33961 ctgactctac tatatctgtt gaaacaccgc taccattcca actgcgacca tctcggctaa
    34021 tgcgcacatg gctttcaccc tgggcatcag ttcccgtatc aatcgcgtca acaatttgca
    34081 agctaaagtc ttcgagttta atttcactcc caccaacaat tttatctatc gcattgagtg
    34141 aagcatcgac aggaccatct ccaatcgaaa ccttttctat aagttctccg tccttgttaa
    34201 taagacgaac aacactagtc gaagtaattg aatttccaga attaataaca aatcggtcga
    34261 gtctaaagct ttctggaatg ccactgccaa aaccacgaac caaggcttct atatcgcggt
    34321 cagaaactgt tttcttttta tctgcaagat ttttaaaatc aacaaagatt ctttctaaat
    34381 cttcttcact cacagcaaag ccaagataac tcaagcgctc ttcaaaggca tgacgaccag
    34441 aatgttttcc aagcaccatc ttgttgtgtg gaagcccaat tgactcaggc gtcataatct
    34501 cataagttgc cctatttgcc aaaaccccat gctgatgaat accagactca tgagcaaagg
    34561 cattttctcc cacaatagcc ttatttggct gacaacgaac cccagttgcc ttcgaaactt
    34621 ttctcgaggt aggatacaaa cgagtcgtat cgatatttgt cgtcatatta tagtaatctt
    34681 ttctcgtata taaattcata acaagctctt cgagagcagc atttccagcc cgctctccca
    34741 agccattgat cgtgcattct atctgcccag ccccagccat agcagctgcc aagctatttg
    34801 gcacagctaa acccaagtca ttatgacagt gcaccgccaa aaccgccttg tcgatatttg
    34861 gcacattgtt acgaatattt tttatcaatt gagcaaattc ttcaggaacc gcatagccca
    34921 ccgtgtcggg aatattcacc gtcgatgccc cagcgttgat aacgccctcg atcaccttgt
    34981 acataaaatc aggctcactt cgagaggcat cctctaaaga aaactctaca tcaccgcaaa
    35041 gatttcgcgc ataggcaacc atctttaccg cctgctcata aacctgatcg ggtgtttttt
    35101 taagcttata ttccatatgt aagggacttg ttgccaaaaa agtatgaata cgtggatttt
    35161 ctgcatcgcg aacagcatcc caggcagcat ctatatcttt ttccaaagca cgagctaagg
    35221 aagcaaccgc tgagccttta atcgtacgag caattgcttg cacgctttta aaatcgccag
    35281 gacttgcaat agcaaaacct gcttcaatta tatctactcc aagaagagcc agactctctg
    35341 caacttcgag tttatctttt aagttcatgc tgcatccagg agcttgctcc ccatctcgta
    35401 aagtagtgtc gaaaatctta atctgtcttg acatatcttc tccctattgt atttacatac
    35461 agttcttttt gatagtttac agtctagata agatagacag tttatacaag gaaaaatgca
    35521 gttttttttg taagagatag caaaataatg tctgttttat tagattttta cttgataaat
    35581 ataatattgt ctattatact aatcaatatg caaataataa gagaaaaata cataaaaaaa
    35641 gctttagaaa agggaaaaac ctcgacaatt gttctcttag atgggcttcg tggtgcagga
    35701 aagtcgcaac ttttgggaga aataggcaat cggctcaaac aacaacgtcc tcccgttcgc
    35761 gttctcatcc tcaacgccct caccggcata aaaaacgacc gtcatctcct tgaacaagca
    35821 cgcggtttag gggcaggcat atccgccctc ctcatcgata atgcagaata cattgaagat
    35881 ttatataccg ccttgcacgc aatactaaaa acctacaata cacgtatttt tctcaccgga
    35941 cagagaactt caggcttaga gaaaatgatt cgaaactcca gccttccaca aagtatcata
    36001 ccggtctttc cctttagcta caaggaattt ttattgcatc aagaactaag ggaaaaccat
    36061 gcagcctttt taagctatct ttccataggc ggacttccac aatccctcct cctccccgtc
    36121 aacaccaagg aatcaaagga gctacgaaaa ctcatagccg actcatttat cttagaaaat
    36181 attatagaac cctacgctat tcggaacccc atcctcattc gacgtatctt ggaaaaaatt
    36241 gcaaaaactt caggagacca agtttccttt cgaagcataa aagaatcgct cgaaacaaga
    36301 gagcaaagca tatcgaatca aagccttatc gactatctca acttttgtag cgaagcaaaa
    36361 ctcttgtata aaattcccat cgtggatatt aaaacaaaaa aaataataga ggctggctat
    36421 gcctggtatt tttccgacct cggtatgaga aataccttta gcgcaggaaa ttattatcgg
    36481 cttccctttc acttgcaaaa aacagaaaac gaaaaagccc ttgaaaacgc tgttttttta
    36541 tccctcatcg acaagggcta tacgatttca aagggacgca taacaaaggg aagctctttt
    36601 attgaataca tcacctttat cgcagaacga gagggaaaaa aaatctatat tcagatatcg
    36661 cccctttcac aaaacctcca aagccaaata caaaaaaaag aagccctcct ctctataaaa
    36721 gacgcctggc caaaatacct cgtcggcagc gaagaagaag acggactgac aaaagaaggc
    36781 atacaagtga taagcgagag aagtctgttg cttgacggcg gaatctaagc gacacaaaaa
    36841 tctgctatac ctagctatct ttaatacagc ac
//
LOCUS       DAOSSW010000009        32862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_1480_length_32862_cov_6.040540, whole genome shotgun sequence.
ACCESSION   DAOSSW010000009 DAOSSW010000000
VERSION     DAOSSW010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 32862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32862
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1480_length_32862_cov_6.040540"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..592)
                     /locus_tag="PKW26_01720"
     CDS             complement(167..592)
                     /locus_tag="PKW26_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOQ92320.1"
                     /translation="MGPKDENTLKEIAKKMSENFFETALGEKLKTATWVESELAYIHL
                     VENPQTKEHRLIKGSIDLVFKDKESEQYFIIDFKTDSTIQPIKHLTQLYFYKEAVIKM
                     KQIPSTSVRSYIFYLRYNKAIELTKEIEKIQPNLLIENY"
     gene            complement(589..2502)
                     /locus_tag="PKW26_01725"
     CDS             complement(589..2502)
                     /locus_tag="PKW26_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOQ92321.1"
                     /translation="MLQKKWQTGLILLSIFIFFVFSCSSSPKASTTSSRAVSGKPRTF
                     ENAASEGLDKVIALVEKLDPKDDELLIVYVRKDKNYAPWALWLWAFPGGDGAAAWDKT
                     QEWKVEDGMGYMRFKMDGSDLGLKPVGPDGQFGMIPRQVAGWTKDGDDDRVWDSNVSN
                     AVVIFSGDNNTYAAKEYKPKAMTAVLSTTTEIQVTFSGRYGLDLDGGDSGFTVETREG
                     KTYPIKKIVNAESPDDLSKNYARRAIITLGEAVQASDALILKNPIFEGDKQIDSSKLA
                     IEMAEKSLPAKNATLGAAYDAKTKSATFSLWAPTSSKAVLNLYKNDEAENPDYTASMK
                     LDAKTGIWSVLFNEVDPDGLFYDISLTNAKGTVKVLDPYAQSMAAYRNTGGSGRAAVI
                     NMASAKALPRGGMNASYVSLKQREDAIIYEMSVRDFTISSDAKVKEAPGTYLSFIEKI
                     PYLKNLGITHVQLMPVVNFYFTDETDRSYEATGTTNDNNYNWGYDPHNYFTPEGWYAS
                     DARDPYARVAELRTLINELHKAGIGVLLDVVYNHMADTSFLDDIVPGYFFRTNAQGKP
                     TSNSGCGNDVATERIMARKLIVDSVVHWVKEYKVDGFRFDLMGLMDTQTVLDSYNESK
                     KLLHKYQKIFYLA"
     gene            complement(2593..4308)
                     /locus_tag="PKW26_01730"
     CDS             complement(2593..4308)
                     /locus_tag="PKW26_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOQ92322.1"
                     /translation="MKDFVRFSKTITRQFFAFSFIVFILFSSCACQTEGRGKQAESQE
                     LSHLFVWTGDPEYKSPKHSPVSLAYMSNQLEGIEEDWYKDSSFYHIWVKAFNDYDGDG
                     IGDFRGITEKLDYIKNDLGCDAIWLSPIFDCSGKGSAVNYNMHGYDTVDYYEVNDYFG
                     NEEHLMTLIEEAHKRDMKLIFDFVPNHTSNAHPWFILSGKREGGKDDWYLWSEERLSW
                     SPMGSSSTWFSNIDRHQYYYGAFNAGMPDLNFRNYEVREEMKNVVRYWLNKGFDGVRI
                     DAVRYLVEDSGTASGLIDTKATHEFFEELRREVIDPYAELGHPKFMVCEAWITGNRNW
                     LNRYYGAEEAEFNMVFDFDFTGKLQTSIKFKNTAFFDYIAREFTSAKNPVHYGTFLSN
                     HDNVANRPATSFKNESELRLATAVSLLLPSTPFIYYANEIGQEDQPNLQGEDIRLRYP
                     LKWENVEAQIKDSKSLLALHRDLNLLRQNHSALTRGALEPCSVKGGDEVLAYTLGYEG
                     EVILCVYNFSSTKVDECSIRLGNQGEKGQILYVSNDEVDFSFDYGEMQLSNFDARSFA
                     VVLLK"
     gene            complement(4465..6249)
                     /locus_tag="PKW26_01735"
     CDS             complement(4465..6249)
                     /locus_tag="PKW26_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOQ92323.1"
                     /translation="MIKEALYHRIDSEFSYFTSDGSVTLRLRAAKDDKISCTLLYGER
                     YFPGEFPPFARVPMKKKGSGLLFDYYEAIIKPGYERLFYLFEISDSTETVWYFQGDIH
                     DSATAFRRNRYYQIPFALREEAYDVPDWAKTAVMYQIFPDSFATGKRQHSGHAEAYEA
                     FAAGRQLKEKKTQTPQGISKSLHGGNFKGITENVEWIKDLGINCVYLNPIFSAVSYHK
                     YDTNDYKQVDPCFGSDEDFRLLVKTLHENGIRVVLDGVFNHSGIHFFAWQDILKNQEK
                     SQYKDWYYDVSFPVKDWHSKYACFAYVKSMPKLNLAHPEAAAYFAEVGAYWIREYDID
                     GWRLDVANEVHKSFWRTFRTAVRAEKTDALLIGEVWEDAQNWQTYDLFDSAMNYRFTY
                     ACIDFIAHQKMTSKEFGERFVDMTMRYPQPVSDVQMNLLDSHDVERFLYFCEGNLEKQ
                     KLAVALQMTLPGMPSIFAGSERAMNGESEREYRLPFLWPGLTENEAERDAMTDWYKRL
                     IRLRHETKALTSSDISFIATENPALLVFSRGIEKGKRVLVVLNNSNKDCQYAPQIHSP
                     LQSMLTKKEVPVGDILQMPALSVDFFLY"
     gene            complement(6242..8635)
                     /locus_tag="PKW26_01740"
     CDS             complement(6242..8635)
                     /locus_tag="PKW26_01740"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOQ92324.1"
                     /translation="MHYYYLDIYRSSKGCCIIFPKPIVFDSKSNMSAAFTDRLASFLH
                     FHADFNEKSYGLVRGETSLLKDFCFIDASQRLITRNDCLKMILRPAALEFVFDGFSLF
                     FSLLFTNKQDPTLCIRSFSHKNNANLAPQLVLLVGENINTYWKCSSKISGPHDTNVSL
                     RETTTSEKNIIYQEFPVYGNNPLILTGTDSSYTLFISLHTQRHHDYTKSKAFIEKKSF
                     SSKLLKKHGKAIRGFFSSFSFTLDPSEVDKKVKIGNQDFNNALLWSLFSAWMMVTGLK
                     HRGIWAGLPWFCDNWGRDTFIALPGVLLVSGCFEEAKNVIRSFARYQDKNPESPSYGR
                     IPNRYRNKKDVIYNTVDGTLWFIREVWEYAEYTGDKHFLKKMFPVVSLALESDRQRRC
                     DGYGFLTHAHSDTWMDARIENAEPLSPRADRACDIQALWYTALKIGSLMAAILHKQQL
                     SELWEKEAERVKKHFLEFFVAKESLKIADYLHEDGERDESIRPNQLFCVTAPRILHAE
                     NFNTGEKSFVDTKIRAQIGKEVFIKLTMPYGVLSLSQDHEHFHPFHDSSAFYHKDSAY
                     HNGTIWLWNTGPLVETMNLLYAQDAVYELTRSHVEQIVSTEPHRWTSRCAGTLSENMD
                     AYPDRKGMLHPSGTFSQTWSLSEFSRNAFSSYAGIKPSLLFNRIYIRPCFPREWREGH
                     ISFLAGTSLYRLSWSTQNTNKISFSLKREESQKKQKNQSVEIIILLAPEKETRELKEQ
                     RFFLSDGVSCLFSGFIPQTDVEYEFATQLYSMTGSKTPNALKEPNYLYNKIKGIKHD"
     gene            complement(8741..10051)
                     /gene="purD"
                     /locus_tag="PKW26_01745"
     CDS             complement(8741..10051)
                     /gene="purD"
                     /locus_tag="PKW26_01745"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOQ92325.1"
                     /translation="MKVAVIGSGGREHTIAWALAKNPDVEKIICIPGNGGTALMKKAE
                     NYKPENSSLESIVEIVEKEKILWAVIGPEDPLAKGLADMLSARGVSVIGPQKKAAHLE
                     ASKDFAKNFMKKYGVACPKSKSFVDIADAKSYVKTQGLPIVIKADGLAAGKGVVVAHD
                     EKTALEAIDSFMADKSLGDAGNTLVIEDYLQGVEISVLAAVSLSEKTRLDGSSCILPF
                     IPARDHKRLLDAAQGPNTGGMGAIAPVDDLSDADWQAFLDKILEPTLKGLIAENMDYR
                     GFIFFGLMLTKDGPKLLEYNVRLGDPETQVVLPLMDSDFFALCQAIHDGSLQNFSLKW
                     KNAYACAPVVVSGGYPGSYKTGYEIDLSEFSKHSDAGEVFFAGAQLLEGKLLTSGGRV
                     LCVSAFGESFDEAREKAYRGLETITFTDSFYRKDIGLPGAAEST"
     gene            complement(10053..12080)
                     /gene="ftsH"
                     /locus_tag="PKW26_01750"
     CDS             complement(10053..12080)
                     /gene="ftsH"
                     /locus_tag="PKW26_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOQ92326.1"
                     /translation="MNENPKKDPNNENDPFNFFKLSTDPKNDKNKKSPKIPFWAILLG
                     VLIITTILNFVFTLRSDSTLDFTEFKALVSNGVITEVVLDKNYFIGYGPQIRNTDTNI
                     FGTLSPSRETYKTVAIWTEGFLEFLDEKGVAYKAQLTKNNFLLDFLLQWILPFGFIFL
                     MWRFLMKRMGGGMGGGLGGSIFSAGQSRANAVDEGQVTTRFADVAGVDEAKEELIEVV
                     DFLKSPKKYTDIGGKIPKGVLLVGPPGTGKTLLARAVAGEARVPFFRISGSDFVEMFV
                     GVGASRVRDLFKQAREKSPCIVFIDELDAIGKSRVTGYGGGNDEREQTLNQLLVEMDG
                     FDNSTGLILLAATNRPDVLDPALLRPGRFDRQVVVDRPDFIGREAILKIHAKNVKLDN
                     DVDLPSIAKTTSGFSGADLANIVNEAALLAVRGGRKKVAMIDFNEAVEKTVAGLQKKS
                     RVIKEEERRIVAYHETGHALTAAFTEGADKVHKISIIPRGVAALGYTMQMPEDDRYLR
                     TRKELLGEVDVLLGGRAAEKIQFSEISTGAGNDLQRATDILRRMITDYGMSEKFENVA
                     LTQRGGAYGYGGEPQLIREYSEETQRYIDSELARLMAERYTFVTENLMKNKDLLDYIA
                     NRLLEKETIDATEFKEIISAHKNLQEKISEDVLDSGSEKAKSSEKEEQKEA"
     gene            12281..12718
                     /gene="fliS"
                     /locus_tag="PKW26_01755"
     CDS             12281..12718
                     /gene="fliS"
                     /locus_tag="PKW26_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOQ92327.1"
                     /translation="MAYTQAFTAYKQTGVKTATQGKLIIMLYEEAVRQLNTALSLFKT
                     EDSIEAYNIEKLHKATVKTQEIITELMVSLNMNAGGQLAHNLLALYMFFNQELLDATI
                     HHKKSKIHFVRDKMLELKDAWVQADSTTQTRASDMNPAISING"
     gene            12725..13207
                     /locus_tag="PKW26_01760"
     CDS             12725..13207
                     /locus_tag="PKW26_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgN"
                     /protein_id="HOQ92328.1"
                     /translation="MTETLSREEIEQRVAILKRYRKLLEEQRNAFRDYLNVLEKQEES
                     IEAESTEVIVAQAELEHKIVASLSSLHRVTLPLEKLYSEQFSTEDEAIPELKTDLENL
                     KQAVLEQNQKNRDLLKIKMGDIRNQIKTLNNPSFNPYAKKASIYSQNNATASILDVEL
                     "
     gene            13208..>15984
                     /gene="polA"
                     /locus_tag="PKW26_01765"
     CDS             13208..>15984
                     /gene="polA"
                     /locus_tag="PKW26_01765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOQ92329.1"
                     /translation="MKDNTIYIFDSYGLIYRAYYAFVSRPLINPQGQNVSAVHGFFRN
                     LLFVIKKYGATYAAASFDSREPTFRHLMYEEYKANRQKTPEDLHAQVPIIEEILESLG
                     FPILRVSGVEADDIIASLCGHCSSQKRQCRILSSDKDLMQLINDYTRMLSPDKTEVWS
                     EVDEEAVLEKWGVPPHLMLDFLSLVGDSSDNVKGVAGVGEKTALKLLTEYGSLDKILE
                     NAEKIPGAIGKKVVAGKDDALFARKLIALKNDVDVPENLENYEIKNLDFLKAAEVLEK
                     YGLMSIAKSYREWGSREKSAQNAEKNRDAHEDAEGDELLQKSHVANSEESKKPTQKEL
                     VKNSGNYKAIKTKPELEKLIETILTEFSKKGREDFYLAFDTETDGLDTRKAEMLGFSL
                     SYIKGEAFYIPLTQEGSLFSQSSIEKKDALESLAKLFYQPGLQIVMQNAKFDYQVLKT
                     NGLTGDFQCEIFDTMIAAWILDADRGSFSFDALCASELFLETIPFSDIVPKGGSFLDV
                     PLDQAVDYAAEDADLTLQLWKKFEKQLKTEGLLDLFLNLESPLIPILAEMEDEGIHIE
                     KADLEAYSKELSSEIEELSKDIYDLVGHEFNIASPKQLQEVLFVERELPATKKIKTGF
                     STDTSVLEELREHDPIIPKLLEYRQLSKLLSTYVDALPKLADENNRIHTSFMQTGTAT
                     GRLSSRDPNLQNIPIRDEEGRKIRQAFTAPAGRSLISSDYSQIELVVLAHLSQDENLM
                     EAFRSGVDIHKATASLIFSLPLEHVTQDMRRSAKTINFGIMYGMSAFRLSNDLSIPRF
                     QAQQFIDNYFASYPGIQNFIQNTIMEAEDKGYVETMLGRRRYIKQILSGNKNIKAQAE
                     RMAINTPIQGTAADIVKKAMIAVDGALKKQFPRAKLLLQVHDELIFECDTSEVAEIKA
                     LIKKEMEEVVQL"
     assembly_gap    15985..16084
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16085..16529
                     /locus_tag="PKW26_01770"
     CDS             <16085..16529
                     /locus_tag="PKW26_01770"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOQ92330.1"
                     /translation="PDGSINRRHLGRILFANPSLLQKQEGIIHPHVDTKLNAFIDSHP
                     EQNIVLNATLLYKTPVIKRCKAILYVQSPLVFRFFRVIKRDSLPIKQIFHRFLAQKKL
                     FSQYKNLNADILIVRNYRSKKCLEKQVFKRLKQIFNEGKQDYGRK"
     gene            16516..17367
                     /locus_tag="PKW26_01775"
     CDS             16516..17367
                     /locus_tag="PKW26_01775"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOQ92331.1"
                     /translation="MEENKILWIVAAVGAFLLVVLGAALIFYTPKPNTSPQIQNLEGI
                     WTSSRETYQEQKNIGLKQNTEDYSIANPATKPYDPTAENTITIGQNVEQNVQAVQDYR
                     QGYLTEGQSIQNLNLYAQNTKLYSENTTIDLNTVSPISNTNQNSQVSNVKDLNATTTK
                     TNTQTKSSSPAQTSTVKTQAPAKTTSTSQATVKTTTIENRFWIQASSFSSKINAEDLK
                     TALTKENFVSEIFTFTDNKDNLFYRVRIGPYTTKSEAEYWQGQLHKVAALKGTETYIT
                     NSSVAVR"
     gene            17476..>17787
                     /locus_tag="PKW26_01780"
     CDS             17476..>17787
                     /locus_tag="PKW26_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92332.1"
                     /translation="MKTILPYFYFIALMLCVPTFAQEINLNTYMSLNSADTYRLGFSS
                     NHKVFSAAFYAEKKGLADTAGNLSVDKSYFFDDWFLKNAYFKIPSKTIDVSFGSLSYS
                     GM"
     assembly_gap    17788..17887
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17888..18292
                     /locus_tag="PKW26_01785"
     CDS             <17888..18292
                     /locus_tag="PKW26_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92333.1"
                     /translation="TFATKLPYFFQSESTELKQEGSLGYSFFLQEIKLFSKHFSHLSI
                     SFYTSFSHSSLPFASEKKAKLNLSESFTIKIEKLSFEEKISLTYKEKEIENIKTKTSL
                     SFPNCKLSLETEYKIPSLTQLWLFSISYKTRL"
     gene            complement(18339..19328)
                     /locus_tag="PKW26_01790"
     CDS             complement(18339..19328)
                     /locus_tag="PKW26_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ92334.1"
                     /translation="MKLTINEIAKMADVSKSTVSKALNGQKGVSDENRTRILNLVHKL
                     NFQPNASARALAQNRTDTIALVIPHDAGYSLSGAYWVEIITAIATTASRYENNLMIIT
                     TGIDKDIAKPIEAVIRRRNIDGLIIGAEQIDTKSLMSLMLEEIPFVFLGRNSVLQHYS
                     VDVRNTEAAAKVTRLLIDRGYTRIGCIAGPKEYLYTQERVEGFRQVLQSAGLSTERIV
                     YTLYSEDETRKNTSSLLEQYPDLDALFLAAGDDFMLNVLDVLRLKGIDQKNFGIGTFD
                     DFRTMDYLHFPIIAAKQPLLKMGEKTAEILFSLIGNTPPPEMNHFFDVDIILR"
     gene            complement(19351..20310)
                     /locus_tag="PKW26_01795"
     CDS             complement(19351..20310)
                     /locus_tag="PKW26_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOQ92335.1"
                     /translation="MAIFDFPLDKLATYMGTNPCPIDFDVFWKDSLEEMRLIEPNIEC
                     IPAQFQTPFAECFDLYFTGVRNARLHAKLLRPKNQREKGPALLQFHGYNGNAGDWSDK
                     LNYVAAGFTVAALDCRGQGVSEDTGGVKGITSPGLFIRGIDDVPKNMIMRHIMLDTAQ
                     IADILMGFDFVKSNQLCVYGASQGGALALACAALEPRVAKVCAMYPFLSDYQRVWEMD
                     LAKDAYYEITHYFRNCDPEHSREKEIFTQLGYIDIKNMAKWIKGEVLMATALQDRICP
                     PSTQFAVYNRIQSKKNHILYPDYQHEWIPQIADRFYQFMLQWS"
     gene            20723..22045
                     /locus_tag="PKW26_01800"
     CDS             20723..22045
                     /locus_tag="PKW26_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ92336.1"
                     /translation="MEKRKKVLVVILLTAMVWSLCAGGKKDSTTADSKEIAGSIIVLH
                     HRTDIEDQFELYKQQFNKLYPKVSVEFEAITEYEGTVRTRMSTQEYGDVLMMVSTPPV
                     PEDFSRYYEPLGTTKELFAKYDFLESNAQCYYDGYVYGLPINANTGGLLYNKEVFKKA
                     GITSFPKTSEEFYAVLEKIKQSTSAIPFFMNYPSKWTLTQWEGGRMAYSGDPDWANKM
                     IHMDNPFDPGMPHYELYKVMYEVVKRGYCEPDMLTSDWEKSKQQFVDGEIAVMNLGSW
                     AIGQFREIAALSGIDPDVVGYMPYPVSHNGVVYTEPSLDYNIAVNRFSKNKEAAKAWA
                     FWFSNESGYAKDTVSIPALKGGEFPAVLDSFKEVGVVYQTATPAKPGEEGLFDKLDNE
                     AEIGLWQDPQKVRIVDAAMGTSKETFDSIMADWNARWAKARKTLKVTP"
     gene            22179..23054
                     /locus_tag="PKW26_01805"
     CDS             22179..23054
                     /locus_tag="PKW26_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ92337.1"
                     /translation="MKRFNNLTHKKQQSIIAFLFLLVPTTLVIVFGYVPFFSMIEYSF
                     LRWDGLGPKQFIGFKNYIDALTRVENYRVFKVSLYYLVASFVQTIAALFFATILSFNT
                     RGKNFFKGVLFFPHLINGVAIGLIFLYFFRGDGGTLNTFLKFLGFKGETLWLSDPKLV
                     NWSIAFTSVWRYTGYNMVMFLGAIQSIPKEIYEAAELDGANWFDVFWHIILKTIMPII
                     KLMLILAITGSLSVFEAPYIMTGGGNGSETFVIRTVETAFKYNKIGLASAMAILLTII
                     IIFITIIQEKFFGND"
     gene            23064..23927
                     /locus_tag="PKW26_01810"
     CDS             23064..23927
                     /locus_tag="PKW26_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ92338.1"
                     /translation="MINNTNLTPRVFKSTQIYFDLLKYFLLFTATLITLLPILVIFFG
                     AFKTGVEFNSTGPFNLPKSWSLDNFRTVWIKGQMGLAFRNTIFIIGGASLGSILTGTM
                     ASYVIHRFNFRGKKFIKNLFLWIVLIPGTTAQVSRFQIVNALGLYNTPWVAIILAMGT
                     DILAIYIYLQFLDSIPLSLDESAIIDGAGYFRVYFQIIMPLLKPATVTVLIIKSIGMY
                     NDFYTPFLYMPKKSLQVISTILYKFKGPYGSQWEVICAGIVIAIIPTLVLFLALQKQI
                     YNGLVSGAVKQ"
     gene            23924..25000
                     /locus_tag="PKW26_01815"
     CDS             23924..25000
                     /locus_tag="PKW26_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOQ92339.1"
                     /translation="MKKLYGFNLQWMYSVSQLLDTLKNTHIQGSIQPGSKLTKETTKA
                     LLQIIQPDLKALNFMQEMDVNFIRLPLDYRFWIHNFEYDKPHTAFLEKLDECVEAIIS
                     RGFHCSLNLHRAPGYCINGNNEELHNLWLDKIAQDAFVLQWENFARRYKKYSADQLDF
                     DLLNEPPDINQYGMNRENHSAIILRAADAIRAISPDRPITINGLGGGNIALPELAHAG
                     FRMSTRGYQPMAISHYEASWCAETQGLDYPKYPFTNYANKIWTIDTIRKHYAPWKELA
                     EKQVPIHIGEFGCYNKIDNTIALRWFQDLFTVFNELGWGYALWQFEGAFGIVNHGRPN
                     TQYKKIDGYEVDIQLYELFKRGMK"
     gene            25000..27186
                     /locus_tag="PKW26_01820"
     CDS             25000..27186
                     /locus_tag="PKW26_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOQ92340.1"
                     /translation="MNKKQRRRHRFTSNKNLLTLVAFFLVLLAITACTSSIRKNQTIV
                     AKENFVSVKDGKFMLDNEIFRFVGTNNYYMHYSTDKMILDVLNDADNMGFTVLRIWGF
                     QIGPNKDHNSYGLNSPARGKKPGNYGIPEENKSTTLKANQFGYPRDIFERLDYTIAEA
                     GKRGIKLVIAMNNYWADFGGLQQASTWQEWFNLDHPSDFYTNKDCILAYKDYIKTLIT
                     RVNSYTGIPYNEDPTIMTWELMNEPRNPQDTSGKVVTAWAKKMSAYVKSLAPYQLCAV
                     GDEGAFIRTDITGFQNEGSHLYNGTEGTDFDKLIALKNIDYGTFHLYPEAWGIAHEAI
                     QSWGLQFISQHIESGEKIGKPVVLEEYGIGQAGLQNRLAIYDSWNKAMFENNGAGTMV
                     WLLTSSNEYELEEGGDGIYDDYDGFRILNDNSTVSNLLRAYAVQFSGETGFKDLVINK
                     PRVYLLDPARDQEAKGSFQIRAQAVDLNQKIQSVKLFINGEPAPAPNIMQYNQETDAY
                     RIKIHTSTYEDGTRLAIKAVFTLADNTILETETYSIFVANNISYSLIKTYDFTTDTAN
                     ASSLGGYQAEIKQLKHTKLNGGMLEVVCNFPGANEWEELKIKFPTMKEVAEAAKISFT
                     LYMKKDTVFPSSTKKDEASKLPGTQHYLAFDPGWIKTGIGESNTFIKDLPIVTLDDGN
                     EYYKEKIEVAFFGSTSYTELTICPTMGYVQYSGSMFIDDISFFKKD"
     gene            27274..29880
                     /locus_tag="PKW26_01825"
     CDS             27274..29880
                     /locus_tag="PKW26_01825"
                     /inference="COORDINATES: protein motif:HMM:NF036809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOQ92341.1"
                     /translation="MLQKKLNTDWTLKPETASDRFTSGPISLNFPTDIHTELLKAAVI
                     EDPYFEKNELKMLWVGQENWVCTKEFTLEESFLQGRQFISITQADTYVRIFINEKEVG
                     LCNNFFKAWRFEVSEFLQKGQNSIKLVFESNENHAIALANKYPYPVPCSIYDVVSPNR
                     NFVRKPQCHAGWDWGPNLMALGIYSDIVLERTATGFLDYLETSTTKTQSNSTETQDWL
                     VRVTSHFTSLTHTTISHRLDFSGPHIEPISIEKKVKLVPGLNTILTEFTVKNPELWYV
                     IGTSPEDEEAILKTGRPLFKDNPLYQLEVKAGGSKKEKNIGFRTLEVVNEEDKDGISL
                     YFKVNDRALFAKGTNWIPLDALPARQTEERYEYLLESLIHGNMNTIRVWGGGMYEKDF
                     FYDYCDRKGILVWQDFMFACGLYPTDKAFLDSVRDEVRHQILRLKDHPSLALWCGNNE
                     NLGALTWYPESIENRDLYLIDYDRLNEGVIGDEVKKLDPNRIWWPSSPCAGPDRFENN
                     WTNDNMGDMHYWEVWHQKKSFDAYLDIRPRFVSEFGYQSFPSLSTVQSYTSENPINLT
                     HPVMEFHQRSPGGNSIMMENFTRYFRFPKSTEKMLYLSQVQQALAIKTACEYWRSLKP
                     YCMGSLIWQLNDNCPVASWSSIDYTGKWKMLHHEAKKFFSPIALSMYSKDEKLFIFGV
                     NDIQKKLDAKLCLSFYNFSGEKIQEDIQLNVSLEADAVSELFSSEIEKLPKDISSYFL
                     YAKLEVATEKGKTQSYEATQFLDLYKKLDIKEAAIDYTITEKSITELPSDILQMKHSR
                     TSQFFQIDIEVKAPAFFVSLDAASIKGLFSDNLLTILPQEKKRLYFIPETKTSLQKFK
                     KMLKVEDLRSSY"
     gene            complement(29947..31095)
                     /locus_tag="PKW26_01830"
     CDS             complement(29947..31095)
                     /locus_tag="PKW26_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ92342.1"
                     /translation="MSYNENMVRTNFLGNYFTKFLSYFIFIFWTLLTISPLVWMGYAS
                     FKTNEELTISMYSLPHDMFFNKDDEYEVVQPQLNIVYPKKIIKKFGFEVSDYDIINEH
                     LIIIESTSMAPGRRVKVAFLDRSEMSSEINALKNGDRLRVNELPKKYQRQIHWDTVFW
                     NYTSAWVRGGLGGKFLNSIIYTFVSTFFVILFGLMIGYAVSKLKFKKLAALIVGLIGL
                     GYLIDINQLIIPLFLMLSKIGLTDTHLGIILVYSAFGLPMAVLLTSQYIRGLPNSLIE
                     SAYIDGATAFKTFIAIIVPMTVPVIITISIMSGLGIWNEFLLVLVMASKEFTKSLPVG
                     VYSFSSRTGTQLGWQIAALVIATLPIILVYFIFQKRLAEGVAGGALKE"
     gene            complement(31097..32011)
                     /locus_tag="PKW26_01835"
     CDS             complement(31097..32011)
                     /locus_tag="PKW26_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ92343.1"
                     /translation="MKKIKTRDLATFLGFVMPGLALYLFIVAYPIIYSVFLSFTNYNP
                     NIGGKWGFVGLTHYIKMFGERMFWFAFKNNMIVVAVSIFGQIPIGFILAYILYRKQVK
                     AANFFQSMVFLPQFLSMIVVGIMWKMLFMVDGPVARIMQAMSGDPTKQFTLMLDPKTA
                     MIPVGIALIWVYTGFYMIIFLANLQKINDSMIEAAKIDGATETQIFIRIIVPLLAGTI
                     LVSSILAIAGSLKGFNLLFGITTDGLTRLNVEVLPLLMYRVAFNDFTNPLRFAYGAAI
                     SNTVVLISIILIMFSNFIGKKLGTDEEY"
     gene            complement(32256..>32862)
                     /gene="nagB"
                     /locus_tag="PKW26_01840"
     CDS             complement(32256..>32862)
                     /gene="nagB"
                     /locus_tag="PKW26_01840"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002665046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOQ92344.1"
                     /translation="VTFNMDEYVGLPSDHPQSYHRFMWDNFFSHVNIKKENVHILNGM
                     AQDLDAECEAYEKTIASFGGIKLFLGGVGVDGHIAFNEPGSSLSSRTRVKTLTRETIQ
                     VNSRFFGGDISQVPTTSLTVGVGTITDSDEVMILVSGHSKARALKHAVEEGINQMWTI
                     SCLQMHKKAIIVCDEDATDELKVGTLKYFKDIESQNLDNSL"
BASE COUNT        10550 a         6529 c         5823 g         9760 t
ORIGIN      
        1 ctttgccaaa tgcaaaatat gttggactgt aaaatgtaaa attattcatt aatacttcct
       61 tataaataaa ttttataatt aattatacca tatattatta aaaaaaaata gaataatatt
      121 tttcttgtta ctagaaattc taaaaatata aatttataat atgtaactaa taattttcaa
      181 ttaaaaggtt tggctgtatc ttttcaattt cttttgtcaa ttcaattgct ttattatatc
      241 gcaagtaaaa aatataagag cgtacagatg tgctgggaat ttgtttcatt ttgataactg
      301 cttctttata aaaatatagt tgtgtaagat gtttgatcgg ctgtatagta ctgtcagttt
      361 tgaaatctat tataaaatac tgttcgcttt ccttgtcttt aaaaactaag tcgatacttc
      421 cttttatcaa tcggtgttct tttgtttgag gattttcaac taagtgaatg tatgcgagtt
      481 cactttctac ccacgtggct gttttcagct tttcacccaa tgctgtttca aaaaaattct
      541 cgctcatttt tttcgctatt tcttttaatg tgttttcatc tttggggcct atgcgagata
      601 aaatattttt tggtatttgt ggagaagttt ttttgattca ttataagaat caagaacggt
      661 ttgtgtatcc attagaccca taagatcaaa gcggaagcca tcaaccttgt attcttttac
      721 ccaatgtacg acagaatcaa cgataagttt tcgagccata attcgctcag ttgcaacgtc
      781 gttaccacag cctgaattac ttgttggctt tccttgtgca tttgttctaa agaaataccc
      841 tggaacaatg tcatctaaaa agctagtgtc agccatatgg ttatagacaa cgtctaaaag
      901 aacacctata cccgctttgt gtagctcatt tatgagtgtt cgcaattctg caactctcgc
      961 atagggatct cttgcgtcag aggcatacca accttcaggg gtaaaatagt tatgtgggtc
     1021 ataaccccag ttataattat tgtcgtttgt agtaccagtt gcctcgtagc ttctatcggt
     1081 ttcatcagta aagtagaaat ttacgacagg catcaattgg acgtgtgtaa tacctaggtt
     1141 cttgagatag ggaatttttt caataaatga aagataggtc cctggtgctt cttttacttt
     1201 tgcatcagaa gagatagtaa agtcgcgcac agacatttca tagattatag catcttctct
     1261 ttgctttaat gaaacgtatg aagcattcat gccgccacgt ggaagtgctt ttgcagaagc
     1321 catgttgata actgcagcac gacctgagcc accggtattt ctataagctg ccattgactg
     1381 tgcatatggg tcgagtactt ttacagtgcc ctttgcattt gtcaagctta tgtcataaaa
     1441 caagccatcg ggatctactt cgttaaaaag aacagaccaa atacctgttt ttgcatcaag
     1501 tttcatactt gcggtataat ctgggttttc tgcttcgtcg tttttgtaga gatttaaaac
     1561 agcttttgat gaagtaggag cccaaagaga gaatgttgct gactttgttt tagcatcgta
     1621 ggcagcacca agagttgcat tttttgcagg aagtgatttt tcagccattt ctatagcaag
     1681 tttagaagag tctatttgtt tgtctccttc gaaaatagga ttttttaaga taagtgcatc
     1741 agaagcttgt acagcttcac ccaaggtgat tatagcccgt cgagcatagt tttttgacaa
     1801 gtcgtctgga ctttctgcgt ttactatttt tttaatagga tatgtttttc cctcacgggt
     1861 ttcaacagta aaaccggagt ctccaccatc taggtcaaga ccgtaacggc cactaaaagt
     1921 aacttgaatt tctgttgttg ttgaaagcac tgctgtcatc gcctttggct tgtattcttt
     1981 tgcagcgtag gtattgttgt ctcctgagaa aataacaact gcgttagaaa cattgctatc
     2041 ccaaacacgg tcatcgtcgc cgtcttttgt ccaaccagct acctgccgtg ggatcatgcc
     2101 aaattgacca tctggaccaa ctggttttag accaagatct gatccgtcca ttttgaaacg
     2161 catataaccc ataccgtctt caactttcca ttcctgtgtt ttatcccagg cagcagcgcc
     2221 atcgccacca ggaaatgccc aaagccaaag cgcccacgga gcataatttt tgtccttacg
     2281 aacatatacg ataagaagct cgtcatcttt tggatcaagc ttttcaacta atgcaataac
     2341 cttatcaagc ccttctgaag cagcattttc aaacgttcgt ggtttgcctg aaacagccct
     2401 acttgatgta gtgcttgcct tgggactcga tgaacaagaa aaaacaaaaa atataaaaat
     2461 gcttagcagt attaatcctg tttgccattt tttttgtaac acaatgttcc tccaaactaa
     2521 acatttttta agaatactat gtactcttaa agtataagct aaactttcag ccctgtcaaa
     2581 gaaaattaaa ccttatttaa gtaaaacaac agcaaaagag cgagcatcaa aatttgaaag
     2641 ctgcatttct ccataatcaa aggaaaagtc cacttcatca tttgaaacat acagtatttg
     2701 acccttctcc ccttgatttc caagacgaat actgcactca tccacctttg ttgatgaaaa
     2761 attatatacg caaaggataa cttctccttc atagccaagt gtataggcta aaacctcatc
     2821 tccacccttg accgaacagg gctctaaagc accccttgtt aaggcagaat gattttgtcg
     2881 caaaagatta aggtctctgt gcaaggcgag aagtgactta gaatctttga tttgcgcttc
     2941 tacattttcc catttgaggg gatagcgaag acgtatatcc tcaccttgaa gatttggctg
     3001 gtcttcttgc cctatttcat tggcgtagta aataaaaggc gttgaaggca ataaaagcga
     3061 aacagccgtt gcaaggcgta gttcactttc atttttaaaa cttgttgcag gtcgatttgc
     3121 tacattatcg tgattggaaa ggaaggtgcc ataatgcaca gggttttttg cactcgtaaa
     3181 ctcacgagct atatagtcaa aaaaagcggt gtttttaaat tttatagagg tttgtaactt
     3241 gccagtaaag tcgaagtcaa aaaccatgtt aaactcagct tcttctgctc catagtagcg
     3301 atttaaccaa ttacgatttc ccgttatcca tgcttcacat accataaact tgggatgccc
     3361 gagttctgca taggggtcta taacctcacg gcgcagttct tcaaaaaact catgagttgc
     3421 ctttgtatct atcaaaccac ttgccgtacc agaatcttct acgagataac gtactgcatc
     3481 tatgcgaacg ccatcgaagc ccttgtttaa ccagtaacgc actacatttt tcatttcctc
     3541 gcgcacttcg tagtttctaa agttgaggtc tggcataccc gcattgaagg ccccgtaata
     3601 atactgatgc ctatctatgt ttgaaaacca ggtgctcgaa gaacccatag gactccacga
     3661 aagccgctct tcgctccata gataccagtc gtcttttcca ccttctcttt tacccgatag
     3721 aataaaccag gggtgagcgt ttgaggtgtg atttggtaca aagtcaaaga taagcttcat
     3781 atctctttta tgagcttctt ctataagcgt cataagatgc tcttcgttcc caaaataatc
     3841 atttacttca taataatcga ccgtgtcgta gccgtgcata ttgtaattta cagcgctacc
     3901 cttgccggag caatcaaaga tgggagaaag ccagatagca tcacagccga ggtcattttt
     3961 tatatagtcg agtttttcgg taataccgcg aaaatctcct atgccgtctc cgtcatagtc
     4021 attgaaagcc tttacccata tatggtaaaa actcgaatct ttataccagt cttcttctat
     4081 gccctctaat tgatttgaca tataagcaag gctcaccgga gaatgcttgg gactcttgta
     4141 ttctggatct cccgtccaca caaaaagatg tgaaagttct tgactttctg cctgctttcc
     4201 tctgccctct gtctggcaag cacaagatga aaaaagaata aaaacaataa aactaaaagc
     4261 aaaaaactgc cttgttatag ttttagaaaa tcgtacaaaa tccttcatta catctcctaa
     4321 tagatttaaa ccggtaaact aaaccggttt atttacatat atattaacgc atgacttttc
     4381 aaatttgtca acttttattt gcattttttt tgactttttt tttaaccaaa agcactatct
     4441 ctaaaaactc tagattttat tgctctagta tagaaaaaaa tcaacagata gggctggcat
     4501 ttgcaaaata tctccaactg gcacttcttt ttttgtcagc atactttgta gcggactgtg
     4561 tatttgaggt gcatactggc agtctttgtt gctattgttt aatacaacga gcacgcgttt
     4621 tcctttttct attccacgac taaaaacaag gagcgctggg ttttctgttg ctataaaaga
     4681 aatgtctgac gaagtaaggg ctttcgtctc atgtcgcaat cgaatgagtc gcttatacca
     4741 atcggtcata gcgtctcgtt ctgcttcatt ttctgttaga ccaggccata aaaagggaag
     4801 tctatattct cgttcacttt caccattcat tgcgcgctct gagcctgcaa atatggaagg
     4861 cattcccggc agagtcattt gtaaggcgac agcgagtttt tgtttttcta ggtttccttc
     4921 gcaaaagtat aaaaaccgct cgacgtcatg actatccaaa aggttcattt gtacatcgct
     4981 tacgggttgc gggtagcgca ttgtcatgtc gacaaagcgt tctccaaatt ctttagaagt
     5041 cattttttga tgggctataa aatctataca ggcataggta aagcgatagt tcatagcaga
     5101 gtcaaagaga tcgtatgttt gccaattttg tgcatcttcc caaacttcac caatgagaag
     5161 ggcatctgtt ttttctgcac gaactgcagt gcgaaatgtt ctccaaaaac ttttatgcac
     5221 ttcgtttgcg acatcaagcc tccaaccgtc tatatcgtat tcacgaatcc agtaggcacc
     5281 aacttctgca aaatatgctg cggcttcagg atgggcgagg ttgagttttg gcatgctttt
     5341 aacataggca aaacatgcgt atttggaatg ccaatcttta acaggaaagg atacatcgta
     5401 gtaccagtct ttatactgtg atttttcttg gttttttaaa atatcttgcc atgcaaaaaa
     5461 atgtattccc gaatgattaa aaacaccatc gagaacaaca cgaataccat tttcatgtaa
     5521 ggtttttact aaaagtcgaa aatcttcatc gcttccaaag caagggtcaa cctgcttgta
     5581 atcatttgtg tcgtacttat ggtaggaaac agcagaaaaa atagggttta gatagacaca
     5641 attaatgcca aggtctttta tccattccac attttctgtt atgcccttga agttaccccc
     5701 atgcaagctt ttacttatgc cctgtggtgt ttgtgttttt ttttctttta actgtcttcc
     5761 agcagcaaag gcttcgtatg cttctgcatg tcctgaatgc tgtctctttc ctgtggcaaa
     5821 gctatctgga aaaatttgat acataacagc tgtttttgcc caatcgggta catcataagc
     5881 ttcttcacgc aaggcaaagg gaatttgata atatcgattt cggcgaaaag ctgttgcgct
     5941 gtcatgtata tcaccctgaa aataccaaac tgtttcagtg ctatcagata tttcaaataa
     6001 ataaaaaagc cgctcatagc caggtttaat aattgcttca tagtaatcaa acaaaagtcc
     6061 gctacctttt tttttcattg ggactcgggc aaagggggga aattctcctg gaaaatatcg
     6121 ctctccgtac aaaagagtgc aagagatctt gtcatctttt gcagcgcgca agcgcaatgt
     6181 aacggagcca tcacttgtaa agtaagaaaa ttcgctatcg atgcgatgat acaaggcttc
     6241 tttaatcatg ttttattcct ttaattttgt tatataaata atttggttct ttaagggcgt
     6301 ttggggtttt cgatccagtc atggaataga gctgcgttgc aaactcatac tctacatcgg
     6361 tttgtggtat aaatcctgaa aaaaggcagg agactccgtc tgaaagaaaa aatctttgtt
     6421 cttttaattc tctcgtttct ttttcagggg cgagtaaaat aattatttca actgattgat
     6481 ttttttgttt tttttggctt tcttctcttt ttagactaaa gcttatttta ttagtatttt
     6541 gtgtgctcca gctaagtcga tacaggctcg ttcccgctaa aaaagaaata tgcccttccc
     6601 gccattcacg aggaaaacag gggcgtatat agattcgatt aaacagtaat gatggtttaa
     6661 tgccagcata ggaagaaaat gcatttcgag aaaattcaga gaggctccat gtttgagaaa
     6721 aagttccaga agggtgaagc attccttttc tatctggata ggcatccatg ttttcactca
     6781 aggttccggc gcaacgacta gtccatctgt gtggctcagt agatactatc tgttctacat
     6841 gcgagcgtgt aagctcatat acagcatctt gtgcatacaa aagattcatt gtttccacca
     6901 aaggacctgt attccaaagc catatagttc cgttatggta tgctgaatct ttatgataaa
     6961 aggctgaact atcatgaaat ggatggaaat gctcatggtc ttgagataaa gaaaggactc
     7021 catagggcat agttaatttt ataaatactt cttttccgat ttgagctctt atttttgtat
     7081 ctacaaagga cttttctcct gtgttgaaat tttctgcatg taaaatcctt ggagccgtta
     7141 cacaaaagag ttgatttggc cgaatacttt catcccgttc accatcttcg tgaagatagt
     7201 ctgctatttt taagctttct tttgcaacaa aaaattccaa aaagtgtttt ttaactcgtt
     7261 cggcttcttt ctcccatagc tctgaaagct gctgtttatg gagaattgct gccataagcg
     7321 aacctatttt caaagcggta taccagagtg cttgaatatc acaagctcta tctgcccgtg
     7381 gagaaagcgg ttcggcattt tctatacgtg cgtccatcca tgtgtcagaa tgtgcatgtg
     7441 ttaaaaaccc atagccatcg caacgacgtt ggcggtcaga ttcaagcgca aggctaacaa
     7501 cggggaacat tttttttaga aagtgcttgt ctcccgtata ctccgcatac tcccatactt
     7561 cgcgaataaa ccaaagtgtg ccatcaacag tattgtatat aacatctttt ttatttcgat
     7621 agcgatttgg tatacgaccg tagcttgggc tttcggggtt tttatcttga taccttgcaa
     7681 atgatctaat gacgtttttt gcttcttcaa agcaacccga aactaaaagc actcccggta
     7741 aagcgataaa ggtatctcgc ccccagttat cgcaaaacca ggggagtccc gcccaaatac
     7801 cacgatgttt taagccagtt accatcatcc atgcgctaaa aagcgaccac aggagagcat
     7861 tgttaaaatc ttggttccca atttttactt ttttatctac ttcgcttggg tctaatgtaa
     7921 aagaaaaaga tgagaaaaaa cctcgaatag cttttccgtg tttttttaag agctttgatg
     7981 aaaaggattt tttttctata aaagccttgc ttttagtgta gtcatgatgt ctttgtgtat
     8041 gtaaagaaat aaagagggta tatgatgaat ccgtgccagt taaaataagt gggttgttgc
     8101 catagacagg aaactcttga tagatgatat ttttttcgct cgtagttgtt tctcgcaaag
     8161 aaacatttgt atcatgtgga ccgcttattt tggaactaca tttccagtaa gtgtttatat
     8221 tttcccctac aagtaaaact aactgaggtg ctaagttagc attgttttta tgtgaaaaag
     8281 aacgtatgca aagtgttgga tcttgtttat ttgtaaaaag gaggctaaaa aataaggaaa
     8341 atccgtcaaa tacgaactca agcgcagcag ggcgtaaaat catcttgagg caatcatttc
     8401 gcgtaataag cctttgtgat gcatctataa aacaaaaatc ttttaaaaga cttgtttctc
     8461 cacggacaag cccatacgat ttttcattaa aatccgcatg gaaatgcaaa aaagaagcaa
     8521 gtctgtctgt gaaagcagca gacatgtttg attttgaatc aaatacaata ggttttggaa
     8581 aaataataca gcatcctttt gaacttctgt agatatcaag atagtaataa tgcatactac
     8641 taaaccggtt tacgcattca tgatacacta ttctagcgat ttgtcaatgt ctttttttat
     8701 agattttttt agtgatcaaa cgaaagcgat agtctctttt ttaagtagat tcggcagctc
     8761 ctggcaaacc tatatctttt ctataaaagg aatcggtaaa agttattgtt tccaagccac
     8821 ggtaggcttt ttcccgcgct tcatcaaagc tctctccaaa ggctgaaaca caaagcactc
     8881 tccccccact tgttagcagt ttgccttcta gcaattgtgc tccagcaaaa aagacttccc
     8941 ctgcatctga atgttttgag aattcagaaa ggtctatttc gtagccggtt ttatatgagc
     9001 caggataacc tccagaaaca acaacgggtg cacaagcgta tgcatttttc catttcaagc
     9061 taaagttttg taaacttccg tcatggattg cttgacaaag tgcgaaaaaa tcggaatcca
     9121 tgaggggtag gactacctgt gtttcagggt ctccgagacg cacattgtat tcgagcagtt
     9181 taggaccatc ttttgtaagc ataagcccaa aaaagataaa accgcggtaa tccatgtttt
     9241 ctgcgattaa gccttttaaa gtgggctcta aaattttatc tagaaaagct tgccaatcag
     9301 catcgcttag atcgtcgacg ggagctatcg cacccattcc accggtattg ggaccttggg
     9361 ctgcatctaa aaggcgttta tggtcgcgtg ctggtataaa gggcaaaatg caagagcttc
     9421 catcgagtcg tgttttttca ctcaaactca ccgctgccaa aaccgaaatt tcaacccctt
     9481 gtaaatagtc ctctataacg agcgtatttc ccgcatcacc gaggctttta tcggccataa
     9541 aggaatcaat agcttcaagc gcggtttttt catcatgtgc aacaacaaca cccttccctg
     9601 cggcaagacc atcagccttt atgacgatgg gtagaccttg cgtttttaca taggacttcg
     9661 catccgcaat gtcgacaaag cttttacttt tcgggcaggc aactccgtac tttttcataa
     9721 agttttttgc aaaatctttg ctcgcttcga ggtgagcagc ttttttttga ggaccgataa
     9781 ccgaaacgcc tcgggctgaa agcatgtctg caagcccctt cgccagggga tcttccggcc
     9841 caattaccgc ccaaagaatt ttttcttttt ctacaatctc tacgatagat tctaagcttg
     9901 aattctctgg tttatagttt tccgcttttt tcattaaggc ggtaccaccg tttcccggta
     9961 tacagattat tttttctaca tcgggatttt ttgccagggc ccatgcaatc gtatgttctc
    10021 gcccaccaga gccaataact gcaactttca ctttatgctt ccttttgctc ttctttttcg
    10081 ctactttttg ctttttcact ccctgaatcg aggacatctt cagagatttt ttcctgcaag
    10141 tttttatgtg cagaaataat ttctttaaac tctgttgcat cgattgtttc tttttctagc
    10201 aaacgatttg cgatataatc gagcaagtcc ttatttttca tgagattttc tgtaacaaaa
    10261 gtatatcgct ccgccataag tcgtgcgagt tctgagtcaa tatagcgctg tgtttcttct
    10321 gaatattctc gtataagttg cggttctcca ccatagccat aggcaccacc acgttgggta
    10381 agagccacat tttcaaattt ctcgctcata ccgtagtcag ttatcatgcg gcgaagaatg
    10441 tcggttgctc tttgtaagtc gtttccggca ccggtcgaaa tttcgctaaa ctgtattttt
    10501 tcagcagcac ggccaccaag gaggacatct acttcaccta aaagttcttt ccgcgttcgc
    10561 aaatagcgat catcttccgg catttgcata gtatagccga gtgctgcaac tcctcgaggt
    10621 ataatcgaaa ttttatgcac cttatccgca ccttcggtaa aggcagcggt taaagcatga
    10681 cctgtttcgt ggtaggctac gatacgtcgc tcttcttcct taatcacacg gcttttcttt
    10741 tgcaaacctg caactgtttt ttcaacagct tcgttaaagt caatcatagc cacttttttt
    10801 cgcccaccgc gaacagcaag caaggctgct tcatttacga tatttgccaa atctgccccg
    10861 gaaaaaccag aggttgtttt tgcaattgaa ggtaaatcaa cgtcattatc aagttttaca
    10921 ttttttgcat ggattttaag tattgcctct cggccaataa aatctggtcg gtcgacaaca
    10981 acttgtcggt caaaacgtcc gggtcgaagc agggctggat ccaaaacatc tggtctattt
    11041 gttgcagcaa gtaaaataag ccccgttgag ttatcaaaac cgtccatttc taccaaaagc
    11101 tggtttaggg tttgctctcg ctcatcgtta ccaccgccgt aaccagttac ccggctttta
    11161 cctatagcat caagctcatc tataaagaca atacaaggag atttttctcg cgcttgctta
    11221 aataagtctc gcacgcggct tgcaccaaca ccgacaaaca tttctacaaa atcacttcct
    11281 gaaattctaa aaaaaggtac gcgtgcttct cctgcaactg cccgtgcaag aagagttttc
    11341 cctgttcctg gaggaccgac aagtaagaca cccttgggta tcttgccacc gatatctgta
    11401 tattttttgg gtgatttaag aaaatctacc acctctataa gctcttcttt tgcctcatcg
    11461 acgcctgcaa catcggcaaa ccttgtggtc acctgcccct catctaccgc attcgctcgc
    11521 gattgcccag ctgaaaaaat actgccaccg agaccgccgc ccatgccacc acccattcgc
    11581 ttcattaaaa aacgccacat gagaaagata aaaccgaagg gaagtatcca ttgcagtaaa
    11641 aaatcgagga ggaagttgtt ttttgtcagc tgcgccttgt aagcaacacc tttttcatct
    11701 aaaaactcca aaaacccctc agtccaaata gcaaccgttt tatacgtttc tcgagaaggc
    11761 gaaagcgtac caaagatatt tgtatctgta tttcgaattt gaggaccata gccaataaaa
    11821 tagtttttat cgagcacaac ctctgttata acgccatttg acacaagggc tttaaattca
    11881 gtaaagtcta aggtagaatc tgaacgcaac gtaaagacaa agtttagaat agttgttata
    11941 attaaaacac cgagtaagat tgcccaaaag ggaatctttg gtgatttttt atttttatca
    12001 tttttgggat ccgttgaaag cttaaaaaaa ttaaaaggat cgttttcgtt atttgggtct
    12061 ttttttgggt tttcgttcac ctaatatctc catttatttt ttgcaaacat atcgagccgc
    12121 tttgttttag ctattgcact atatacattt tgcatcttct ttaaatatac gtaataaagc
    12181 aaaaatagtc gagtacatga gcaaaattcg ggtctttttt cttatgttct tttttagaat
    12241 gccgatatat aaatgaatag atgctattgg gaggtttttt atggcatata ctcaagcctt
    12301 tactgcttac aaacaaacgg gagttaagac tgctactcaa ggaaaactca ttatcatgct
    12361 ctacgaggaa gcagttcgcc aactaaatac cgctttaagt ctgtttaaaa cagaagattc
    12421 tatagaagcg tacaatattg aaaaattaca caaagctacg gtaaaaaccc aagaaattat
    12481 cacagagctg atggtttcac tcaacatgaa cgccggcgga caacttgccc acaaccttct
    12541 cgccctatac atgtttttta accaagaact tctcgacgct accatccatc ataaaaaaag
    12601 caaaatccac tttgtccgcg acaaaatgct tgagctaaaa gatgcttggg tacaggcaga
    12661 ttctacaaca caaacccgtg cttccgacat gaaccctgca atcagtatca acggatagat
    12721 agctatgacc gaaacactca gtcgagaaga aatagagcaa agagtcgcaa ttttaaaacg
    12781 atatagaaaa cttttggaag agcaacgaaa tgcctttcgc gactatctca atgttctcga
    12841 aaagcaagaa gaaagcatag aagcagaaag caccgaggta atcgttgctc aagctgaact
    12901 tgaacataag atagtcgctt ccttgtcgag tttacatcgt gtaacgcttc cccttgaaaa
    12961 actctactct gagcaatttt ccacagaaga tgaagctatt ccagagctca agacagacct
    13021 tgaaaatctc aagcaggcgg ttttagagca aaaccaaaaa aacagagatt tattaaaaat
    13081 aaagatgggt gatatccgaa atcaaataaa aacgctcaac aaccccagct tcaatcccta
    13141 tgcaaaaaaa gcgagtatct actcgcaaaa taatgccaca gctagtatac tagatgtgga
    13201 gttataaatg aaagacaata caatatatat tttcgattcg tatggcttga tttatcgagc
    13261 ctattatgcc tttgtgtcca gaccgctgat aaacccccaa ggacaaaatg tttctgctgt
    13321 tcatggtttt tttagaaatc ttttatttgt tataaaaaaa tacggcgcca cctacgctgc
    13381 agcaagtttt gactcacgag agccaacctt tcgccatctc atgtatgaag aatacaaggc
    13441 gaataggcaa aaaaccccag aagacttaca tgcacaagtt cccatcatag aagaaattct
    13501 cgaaagcctt ggcttcccca tcctacgagt aagcggtgtt gaggcagatg atattattgc
    13561 gagcctttgt ggacattgta gcagtcaaaa aagacagtgc cgcatcctca gttccgacaa
    13621 agacttaatg caactgataa acgactatac acgtatgctc agtcccgaca aaaccgaagt
    13681 gtggagcgag gtcgacgagg aagctgtgct ggaaaaatgg ggcgttcccc cgcatctcat
    13741 gctcgatttc ctctccctcg ttggagatag ctctgacaat gtgaaaggtg ttgcaggagt
    13801 tggtgaaaaa acagctctta aactcctcac cgaatacggc agcctcgata aaatcttgga
    13861 aaatgctgaa aagataccag gtgcaattgg aaaaaaagtt gtggcaggaa aagacgatgc
    13921 tctctttgct cgtaaactca ttgctttaaa aaatgacgta gatgtgcctg aaaatcttga
    13981 aaactatgaa ataaaaaatc tcgacttttt aaaagctgcc gaagtccttg aaaaatacgg
    14041 tttaatgagc attgcaaaaa gctaccgcga atggggaagt cgagaaaaaa gcgcacaaaa
    14101 tgcagaaaaa aatcgcgatg cccatgagga tgcagaaggc gatgaacttt tacaaaagag
    14161 ccacgttgca aacagtgaag agagcaaaaa accaacacaa aaagagcttg taaaaaacag
    14221 cggaaattac aaggctataa aaacaaaacc tgagctagaa aaactcatcg agacaatttt
    14281 gacagagttt tctaaaaagg gaagagagga tttttatctt gcctttgata cagaaaccga
    14341 tggcttagac acaagaaagg cagagatgct cggtttttct ctttcatata tcaagggaga
    14401 agccttttat attcctctca cacaagaggg tagccttttt tcacaaagca gtatagaaaa
    14461 aaaagatgct ctcgaaagtc ttgcaaagct tttttaccag cctggtcttc aaattgttat
    14521 gcaaaatgca aagtttgatt atcaagtatt aaagacaaat ggtttgacgg gagattttca
    14581 gtgcgaaatc tttgatacta tgattgcagc ctggatctta gatgccgatc gcggctcttt
    14641 ttccttcgat gccctttgtg cttcggagct ttttttagaa accattcctt tttcggacat
    14701 tgtccccaag ggtggcagtt ttttggatgt tcccctcgat caagctgtcg actatgccgc
    14761 agaagatgcc gacctcacct tgcaactatg gaaaaagttt gaaaagcaac tcaaaactga
    14821 gggcttgctt gacctatttc taaatcttga aagccccctt attcccatcc ttgcagagat
    14881 ggaagatgaa ggcatacata ttgaaaaagc agatttggaa gcctactcca aggaactttc
    14941 aagcgaaata gaagagctat caaaggacat ttatgattta gtcgggcatg agttcaatat
    15001 tgcttcgcca aaacaattgc aagaggtgct ctttgtagag cgtgaacttc ccgcaacaaa
    15061 aaaaataaaa acaggttttt caaccgacac ctctgtttta gaagagctac gcgagcatga
    15121 ccccatcatt cccaaacttc tcgaataccg tcagttaagc aagcttttga gcacctacgt
    15181 cgatgccctg ccaaaactcg ccgatgaaaa taatcgcatt cacacaagct ttatgcaaac
    15241 aggaacagca acagggcggc tttcaagtcg cgaccctaac ttacaaaata ttcccattcg
    15301 cgacgaggaa ggacggaaaa tacgtcaagc ctttactgcg cctgctggaa gaagccttat
    15361 ttcctcggac tattcgcaaa tagaacttgt agtactcgct catttaagcc aagacgaaaa
    15421 ccttatggaa gccttccgct ctggagtgga tattcataag gcaaccgcca gccttatttt
    15481 cagccttccc ctcgaacacg tgacccaaga tatgagaaga agcgcaaaaa cgataaactt
    15541 tggcattatg tatggcatgt cagccttccg cttatcaaat gacctttcta tcccccgttt
    15601 tcaagcacaa caatttatag ataattattt tgcaagctat cccggaattc aaaactttat
    15661 tcaaaacacc atcatggaag ctgaagacaa gggctacgtg gaaacgatgc tcggaagacg
    15721 acgctatata aaacaaattt taagcggaaa taaaaatatc aaagcgcagg cagaacgcat
    15781 ggcgataaac acacccatcc aaggcactgc cgccgacatt gtcaaaaaag cgatgatcgc
    15841 cgtagatggg gctttgaaaa aacaattccc aagggcaaaa ctcctcttac aagttcacga
    15901 cgagttgatt tttgaatgcg atacttccga agtagcagag ataaaagccc tcataaaaaa
    15961 agagatggaa gaagttgtcc aactnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16081 nnnntcctga tggaagcatc aatcgaaggc atttaggacg catccttttc gcaaatcctt
    16141 ctcttttgca aaaacaagaa ggcattatcc atccccatgt agatacaaaa ctcaatgcct
    16201 ttatagacag ccaccctgag caaaatatcg tactcaatgc aacacttctc tataaaacac
    16261 ccgttataaa acgctgcaaa gcaattcttt acgtccaaag tcccctagtc tttcgctttt
    16321 ttcgtgttat caaacgcgat tcccttccaa taaagcagat tttccataga tttttagcac
    16381 aaaagaaact cttttctcaa tataaaaatc taaatgccga tatattgata gtacggaact
    16441 accgttcaaa aaaatgttta gaaaaacaag ttttcaaacg tttaaaacag attttcaatg
    16501 aagggaagca agactatgga agaaaataaa atactatgga tagtagctgc ggttggtgca
    16561 tttttactcg ttgttttagg agccgccctt attttttata ccccaaaacc aaataccagc
    16621 ccacaaatac aaaaccttga aggcatatgg acaagttcac gcgaaaccta tcaagagcaa
    16681 aaaaatattg gcttaaagca aaatacagaa gattactcta ttgcaaaccc tgcaacaaag
    16741 ccctatgacc caacagcaga aaacacaatc acaataggac aaaatgtcga gcaaaatgta
    16801 caagctgtac aagactatag acagggatat ctcactgagg gacagagcat acaaaacctc
    16861 aacctctatg cacaaaatac aaagctctac tctgaaaaca caacaattga cttaaatact
    16921 gtatcaccta tctcgaacac aaatcaaaac agccaagtct caaatgtaaa agacttaaat
    16981 gccacaacta caaaaacaaa tacacagaca aaatcatcaa gccctgcaca aacaagcact
    17041 gtgaaaacgc aagcgccagc caagacaaca agtacaagcc aagcaacagt aaaaacaacg
    17101 acaattgaga ataggttttg gatacaagct tcttcatttt cgagcaaaat aaatgccgaa
    17161 gacttaaaaa ctgcactcac aaaagaaaat tttgtaagcg agatttttac cttcaccgac
    17221 aataaagaca atttatttta ccgtgttcgc ataggaccct atacaacaaa atctgaagcc
    17281 gaatactggc agggacaatt acacaaggtc gcagcgctca agggaactga aacctatatc
    17341 accaattcat cagtagctgt ccgataggca ataaaaaaac gatagcaaaa cacgaacatc
    17401 aaaaaaactt tttttatttt tttattttaa ttaaaaataa aaaaaacacc cactaaaaaa
    17461 ccaataaggt agtatatgaa aacaatacta ccttattttt atttcatagc attgatgcta
    17521 tgcgtcccaa cttttgccca agaaataaac ctcaatacct atatgtcgct aaattctgcc
    17581 gatacctata ggcttggttt ttcaagtaat cacaaagtat tttcagcagc cttttatgca
    17641 gaaaaaaaag gtcttgcaga tacagcagga aatctgagtg tagataaaag ttattttttc
    17701 gatgactggt ttctaaaaaa cgcgtatttt aaaattccaa gcaaaacaat cgatgtgagt
    17761 tttggttctc tcagctactc aggtatgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnact tttgcaacga agcttcctta tttttttcaa tcggaaagca cagaactgaa
    17941 acaagaagga agccttggct atagtttctt tttacaagaa ataaagcttt tttccaaaca
    18001 tttttcccat ctaagcatca gtttctatac ttccttttcg catagcagcc ttccctttgc
    18061 ctccgaaaaa aaagcaaagc tcaatctaag cgaaagcttt acaataaaaa tagaaaaact
    18121 ctcttttgaa gaaaaaattt ctttgacata taaagaaaaa gaaattgaaa atataaaaac
    18181 aaaaacaagc ttaagttttc ctaactgcaa actaagcctt gaaacagaat ataagatacc
    18241 ctcattaaca cagctatggc ttttttctat ttcttataaa acaaggcttt gaaggcgcat
    18301 ataaatttat aaaaaaatct atttataaac actcatcctt accgtaaaat aatatcaaca
    18361 tcaaaaaaat gattcatttc cggtggtggg gtatttccaa taagagaaaa aagaatttct
    18421 gctgtctttt cgcccatttt taaaaggggt tgctttgctg caattatagg gaaatggaga
    18481 taatccattg ttctaaaatc gtcaaaagtt ccaataccaa aattcttttg atcaattcct
    18541 tttaaacgca aaacatctaa aacattcaac ataaaatcat caccagcagc taaaaaaaga
    18601 gcatctagat ctggatattg ttcaagcaaa gaacttgtat tttttcgagt ttcatcttca
    18661 gaatacaagg tatacacaat tcgctctgta cttaaaccag cactttgaag tacttgacga
    18721 aaaccttcaa cacgttcttg cgtatataaa tattcttttg gtcccgcaat acaaccaata
    18781 cgtgtatagc cccggtctat aagcaaacgc gttacctttg cagcagcctc ggtattacgc
    18841 acatctacag aataatgttg taaaacagaa tttcgaccta aaaaaacaaa aggaatttct
    18901 tcaagcataa gcgacataag gctttttgta tcaatttgct ctgctcctat aataagtccg
    18961 tcaatatttc gtctcctgat aacagcctct attggttttg caatatcttt atcaattccg
    19021 gtggtaataa tcataagatt gttttcataa cgagatgctg ttgttgctat tgctgtaata
    19081 atttctaccc aatacgcacc agaaagacta taccctgcat cgtgcggaat gactaaagct
    19141 atggtatctg ttctattttg tgctaatgca cgagcagaag catttggctg aaaattaagc
    19201 ttgtgaacta aattaagaat tcgagtacga ttttcatcgc taacaccctt ttggccattg
    19261 agtgcttttg aaacagtact ttttgacaca tctgccattt ttgctatttc attaattgtc
    19321 aatttcattt tgcactcctc ttttcttatg ttagctccat tgtaacataa attgataaaa
    19381 tcggtcagct atttgaggta tccactcatg ctgataatca ggataaagaa tatgattttt
    19441 tttagattgt atgcgattat aaacagcaaa ttgtgtagaa ggagggcaaa ttctatcttg
    19501 caaagctgtt gccataagta cctctccttt aatccatttc gccatatttt ttatatcaat
    19561 ataaccgagt tgtgtaaaaa tctctttttc tcggctatgt tctggatcac aatttcgaaa
    19621 ataatgcgtt atttcgtaat aagcatcttt agctaaatcc atttcccaca ctcgctgata
    19681 atcacttaaa aaggggtaca tagcgcatac tttcgcaact cgaggttcaa gagctgcaca
    19741 tgcaagagct aaagcaccac cttgagaagc accatataca cataattgat tactttttac
    19801 aaagtcaaaa cccattagaa tatctgctat ctgcgctgta tcaagcatga tatggcgcat
    19861 tatcatattt ttaggcacat catcaatacc tcgaataaat aaaccaggag aagttatacc
    19921 ttttactccg ccggtatctt cggaaacacc ttgccctcga caatcaagag cagcaactgt
    19981 aaaacctgca gcaacgtagt taagtttatc agaccaatct cccgcatttc cattatagcc
    20041 atgaaattga agcaaagctg gccccttttc tctttgattt tttgggcgca gtaatttagc
    20101 atgaagacgt gcatttcgaa caccagtaaa atataaatca aaacattcag caaaaggtgt
    20161 ttgaaattgc gcaggtatac attcaatatt cggttcaatt aaccgcattt cttcaagcga
    20221 gtccttccaa aaaacatcaa aatctatagg acaaggatta gtacccatat aggtagctaa
    20281 tttatccaaa ggaaaatcaa aaattgccat aacacctcca aatagtttta gatgaacaat
    20341 atagaggtac ctacctgtgt tattcatcta cccatctttc ttatctttct atttaatacg
    20401 attaaataga aaccgatttc tctacagtgt ataatctatt aatacgtctg tcaattaaaa
    20461 taagctaaaa cttacaagct aatatctcta aatagaaaaa ccaaagcaaa tatttagaag
    20521 aaataggtat aaaaataaaa atatttaaat aaatttgagg aaaatataat tttctcttga
    20581 caacatgttt ttctatgata tacttcaagc cagaaaagag aaaccggttt ctctgcagtt
    20641 tatatctttt tatgaaaata tctaaaagtg atataaactg taaaaaaaag gtttcaaaaa
    20701 aaaattccaa aggagaaaac aaatggaaaa aagaaaaaag gtgttagtgg tgatcctgct
    20761 cactgctatg gtttggtctc tttgtgctgg aggaaaaaaa gatagcacaa cagcagactc
    20821 taaagaaatt gcaggctcaa ttatagtttt acatcatcgt actgatattg aagatcagtt
    20881 tgaactttac aaacagcaat ttaataagct gtatcctaaa gtttctgttg aatttgaagc
    20941 cattaccgaa tatgaaggta ccgtaagaac tcgtatgtca acacaagaat acggagatgt
    21001 attgatgatg gtttcaacac cccctgtacc tgaagatttt tctcgatact atgaacctct
    21061 cggaactaca aaagaacttt ttgcaaaata tgatttttta gaatcaaatg cacaatgtta
    21121 ttacgatgga tatgtatatg gtctacctat taatgcaaat actggaggtc tattatataa
    21181 caaagaagtt tttaaaaaag ctggtattac aagtttccca aaaacatccg aagaatttta
    21241 tgcagtcctt gaaaaaatca aacaaagcac cagtgctatt cctttcttta tgaactaccc
    21301 atccaagtgg acacttacac aatgggaagg cggacgtatg gcatattctg gtgatcctga
    21361 ttgggctaac aaaatgattc atatggacaa tccttttgat cctggaatgc ctcattatga
    21421 gctatataaa gttatgtatg aagttgttaa acgaggctat tgtgaaccag atatgttgac
    21481 atcagattgg gaaaaatcaa aacaacaatt tgtcgatgga gaaatcgccg taatgaactt
    21541 gggttcttgg gctataggtc aatttagaga gattgcagcg ctttcgggta ttgatcccga
    21601 cgttgtagga tatatgccct atccagtaag ccacaacggt gtagtataca cagaaccctc
    21661 tcttgattat aacatcgcag taaatagatt tagtaaaaac aaagaagcag caaaggcatg
    21721 ggcattttgg ttttcaaatg aatctggcta tgcaaaagac actgtttcta ttcctgcgct
    21781 aaaaggcgga gaatttcctg ctgttctaga tagttttaag gaagttggtg ttgtatatca
    21841 aactgctacc ccagctaaac ctggcgaaga aggcttattt gataaactag acaatgaggc
    21901 cgaaattggc ttatggcaag acccacaaaa agtacgaatc gttgatgcag caatgggcac
    21961 ttcaaaggaa acctttgatt caataatggc cgattggaat gcacgttggg caaaagcaag
    22021 aaaaacttta aaagttaccc cgtaattgct ttaatactgt aaggaaatta tacacattta
    22081 tatctaacgc atgtgtatta actatttgaa atgctgataa gaataccgct tttggtgttt
    22141 ttatcagcat ttttatacta aattataagg agtatgtttt gaaacggttt aataatttaa
    22201 cccataaaaa acaacaaagt attattgcat ttcttttttt acttgtcccc acaacgcttg
    22261 taattgtttt tggctatgtc ccttttttta gcatgattga atatagtttt ttacgatggg
    22321 atggtctcgg tcctaaacag tttataggtt ttaaaaacta tattgatgca ctaacaagag
    22381 tcgaaaacta tagagtattt aaagtgagct tgtattattt agtagcttct tttgtacaaa
    22441 caatcgcagc actatttttt gccacaatac tttcatttaa tacacgaggg aagaactttt
    22501 ttaaaggtgt attatttttc ccccacttaa ttaatggcgt agctatcggc cttatttttc
    22561 tttatttttt ccgaggtgat ggtggaactt taaatacatt tttaaaattt ctcggattta
    22621 aaggagaaac gctttggctt agtgatccaa aattagtcaa ctggtctatc gcttttacat
    22681 ctgtttggcg ctacacgggc tataatatgg ttatgttttt gggagcaatt caatcaatac
    22741 caaaagaaat ttatgaagca gcagaactag atggcgccaa ctggtttgat gttttctggc
    22801 atattattct taaaactata atgcccataa taaaactcat gctcatcttg gcaataactg
    22861 gttctttaag cgtatttgaa gcaccatata ttatgacagg tgggggaaat ggtagcgaaa
    22921 catttgtaat tcgaacagtt gaaacagcgt tcaaatacaa taaaattgga ttagcatctg
    22981 caatggctat tttattaaca attattatta ttttcataac aataatccaa gaaaagtttt
    23041 ttggaaacga ttaggaatat actatgataa acaacaccaa tcttacacca cgagttttta
    23101 aaagcacaca aatatatttt gacttgctaa aatatttttt attattcaca gctacattga
    23161 ttacgttgct acctatttta gttatttttt ttggcgcttt taaaacaggc gttgagttta
    23221 atagcactgg accctttaat ttgcctaaaa gttggtcttt agataacttt cgaactgtct
    23281 ggataaaggg tcaaatgggt cttgcattta gaaatacaat ttttataatt ggaggcgcaa
    23341 gcttaggttc aatacttaca ggaacaatgg catcgtatgt tattcatagg tttaattttc
    23401 gtgggaaaaa atttattaaa aatttatttt tatggattgt tttaattccc ggaacaaccg
    23461 cacaagtgtc tcgttttcag attgttaacg ctctcggtct ttacaataca ccatgggtag
    23521 caattattct tgcgatgggt acggatatat tggcaattta tatttatcta caattccttg
    23581 acagtattcc tctttccctc gacgaatcag ctattatcga tggagcaggt tattttcgtg
    23641 tttatttcca aataataatg ccattattaa aacctgcaac agtaaccgta cttataataa
    23701 aatcaatagg aatgtacaac gatttttata cgccttttct ttatatgcca aaaaaatcat
    23761 tacaagttat ttctaccata ttatataaat ttaaaggtcc gtatggttca cagtgggaag
    23821 taatatgcgc gggaattgtt atcgcaatta taccaacact agtgcttttc ttagcattac
    23881 aaaaacaaat atataacggc ttagtaagtg gggcagtaaa acaatgaaaa aactttatgg
    23941 atttaatttg caatggatgt attcagtatc acagttatta gacacactaa aaaatactca
    24001 tattcaagga tcaatacagc ctggttcaaa gcttacaaaa gaaacgacaa aggcattact
    24061 tcaaataata caaccagatc tcaaagctct taactttatg caagaaatgg atgtaaattt
    24121 tattcgttta cctctcgatt atcgattttg gatacataat tttgaatatg acaaacctca
    24181 taccgcattt ctcgagaaac ttgatgaatg cgtagaggct attatatcac gcggatttca
    24241 ttgttcgctt aatttacatc gcgcacccgg atattgtata aatggaaaca atgaagaatt
    24301 gcataatctt tggcttgata aaattgctca agatgctttt gttttacagt gggaaaactt
    24361 cgcacggaga tacaaaaagt actctgctga ccaattagat tttgatttac tgaatgaacc
    24421 tccagatatt aatcaatatg gaatgaatcg tgagaatcat tctgctatta tactacgtgc
    24481 tgctgatgct attcgtgcaa taagccctga cagacctatc acaataaacg gtcttggcgg
    24541 aggaaatatt gccctaccag aactcgcgca cgctggattt cgtatgagta cacgaggcta
    24601 tcaaccaatg gctatatctc attatgaagc atcttggtgt gcagaaactc agggacttga
    24661 ctatccaaaa tatcctttca caaactatgc aaataaaatt tggaccatag acaccattcg
    24721 caaacactat gccccatgga aagaacttgc agaaaaacaa gtcccgatcc atattggaga
    24781 gttcggttgc tataacaaaa tcgacaatac tattgcactt cgatggtttc aagacttatt
    24841 tactgttttt aacgagctcg gttggggcta tgccttatgg cagttcgaag gcgcctttgg
    24901 cattgtaaat catggacgac caaatacaca atataaaaaa attgatgggt atgaagtcga
    24961 tattcaatta tatgaattat ttaaaagagg tatgaaataa tgaataaaaa acaaagaaga
    25021 agacatagat ttacttcaaa taaaaatctt ttgactttag tcgctttttt tctagtactt
    25081 ttagctatta ccgcctgtac ttcatcgata cgtaaaaatc aaacaattgt ggcgaaagaa
    25141 aactttgttt ccgtaaaaga tggcaaattt atgctcgata atgagatatt tcgctttgta
    25201 ggtacaaaca actattatat gcactatagc acagataaaa tgatacttga cgttctaaac
    25261 gatgcagata atatgggttt tactgttctc agaatatggg gttttcagat aggtccgaat
    25321 aaagaccata attcatatgg cttgaatagt ccagcacgtg gaaaaaaacc tggaaattac
    25381 ggtatacccg aagagaacaa aagtacgaca ctcaaagcaa accaatttgg ctatcctaga
    25441 gatatttttg aaagacttga ttacactatt gctgaagcag gaaaacgagg tattaagcta
    25501 gtaattgcga tgaacaatta ttgggccgat tttggtggct tacaacaagc ttcaacatgg
    25561 caagaatggt ttaatctcga tcatccgagc gacttttaca ctaacaagga ctgtattctt
    25621 gcatataagg actatattaa aacactaata acccgcgtta attcttacac aggcataccc
    25681 tataatgaag atccgaccat aatgacatgg gaactaatga atgaaccacg aaaccctcaa
    25741 gatacttcag gtaaggttgt tactgcgtgg gcaaagaaaa tgagtgctta tgtgaaatct
    25801 ttagcaccct atcaattatg tgcagttggc gacgagggcg cgtttataag aacagatata
    25861 actggctttc aaaatgaagg aagccatttg tacaatggaa cagaagggac tgattttgac
    25921 aaacttattg cactaaagaa tatcgattat ggtactttcc atttataccc agaggcttgg
    25981 gggattgcac atgaagcgat tcaatcttgg ggcttacaat ttatttccca acatatagaa
    26041 tcaggagaaa aaataggtaa acccgtagtt ttagaagaat atggtattgg acaagctggt
    26101 ttacaaaacc gccttgctat ctatgattca tggaataaag caatgtttga aaataatgga
    26161 gcaggaacaa tggtttggct tcttacttct tcaaatgaat acgaattaga agaaggtggc
    26221 gatggtattt atgacgacta tgacggcttt cgaatactca acgataattc aacagtttca
    26281 aatttactgc gagcatacgc cgttcaattc tctggagaaa ctggttttaa agaccttgtt
    26341 ataaataagc ctcgagtata tttactcgat ccagcacgag accaagaagc caaaggttct
    26401 tttcaaattc gggcacaagc tgtagatctt aatcaaaaaa tacaatcagt aaaactattt
    26461 atcaatggag aaccagcacc tgctccaaat attatgcaat ataaccagga aactgacgca
    26521 taccgtataa agatacatac aagcacgtat gaagacggta caagactggc aattaaagca
    26581 gtatttactt tagcggataa tacgattttg gaaactgaaa catattccat tttcgtagca
    26641 aacaatattt cctactcact gataaaaaca tatgacttca caaccgatac agcgaatgca
    26701 agctccttag gtggttatca ggcggaaata aagcaactta aacacacaaa gctaaatggt
    26761 ggtatgttag aagttgtctg caattttcca ggagctaatg aatgggaaga acttaaaata
    26821 aaatttccca ctatgaaaga ggttgctgaa gctgcaaaga tttcatttac actttatatg
    26881 aaaaaagata ccgtttttcc ttcctctact aaaaaagatg aagcaagtaa attgccagga
    26941 acgcaacatt atttagcctt cgatcccggt tggataaaaa ctggtattgg tgaaagcaat
    27001 accttcataa aagatcttcc tatcgttaca cttgatgacg gaaatgaata ttataaagaa
    27061 aaaattgaag ttgctttttt tggaagcact tcatacacag aacttacaat ttgccccaca
    27121 atgggatatg tacaatattc aggttccatg tttatagatg acatttcttt ttttaaaaag
    27181 gattaaaggg tgtactaatc gcctccggct tctttcttta cagaaaggcc taagacatgc
    27241 tactatgata caaattcaca gaaaggaaag gaaatgctac aaaaaaaact caatacagat
    27301 tggacactga aaccagagac ggcgtcagat cgctttactt ctggccctat ttctctcaat
    27361 tttcctacag atatacatac agagctcctt aaggctgctg ttatagaaga cccatacttt
    27421 gaaaaaaatg aactgaaaat gttatgggta ggacaagaaa actgggtatg cacaaaagaa
    27481 ttcacccttg aagaatcttt tttacaaggc agacaattta tttccataac ccaagctgac
    27541 acctatgttc gtatttttat caacgaaaaa gaagtcggac tttgcaacaa tttttttaaa
    27601 gcatggcgct ttgaagtaag tgaatttttg caaaaaggac aaaacagcat caagcttgta
    27661 ttcgaatcaa acgaaaatca tgccattgca cttgcaaaca aataccccta ccctgttccc
    27721 tgttctattt acgatgttgt ttcaccgaac cgaaactttg tccgtaaacc acaatgtcat
    27781 gcaggctggg actggggtcc aaacctcatg gcactaggta tttacagcga catagtcctc
    27841 gaaagaaccg ccacaggctt tctcgactat cttgaaacca gcactacaaa aacacaaagc
    27901 aatagcacag aaactcaaga ttggctggtt cgtgttacat cacattttac ctcgcttaca
    27961 catacaacaa tttctcatcg actagacttt agcgggcctc atatcgagcc cataagcatc
    28021 gagaaaaaag taaagctcgt ccccggctta aatacaattc ttacagaatt caccgtaaaa
    28081 aacccagaac tctggtatgt cataggaaca agcccagaag atgaagaggc aatactaaaa
    28141 acggggcgac cgctttttaa ggataatcct ttatatcaac ttgaagtaaa agctggggga
    28201 agcaaaaagg aaaaaaacat aggttttaga acgcttgaag ttgtaaatga agaagacaaa
    28261 gatggtatca gtctttattt taaagtgaat gacagagcgc tttttgccaa agggacaaac
    28321 tggattccac tcgatgcgct cccagctcgt caaacagaag agcgctatga atacttactc
    28381 gaatccctca ttcacggaaa catgaacaca atacgcgttt ggggcggtgg tatgtatgaa
    28441 aaagattttt tctacgatta ctgcgacaga aagggtattc tcgtttggca agattttatg
    28501 tttgcctgtg gtctctatcc aaccgacaag gcgtttttag attcagttcg agatgaggtt
    28561 cgccaccaaa tccttcgcct aaaagatcac ccttctttgg ctctttggtg tggaaacaac
    28621 gaaaaccttg gcgcactgac ctggtatcca gaatccatag aaaaccgtga tttatattta
    28681 atcgattacg accgactaaa cgagggcgtc ataggagacg aagtaaaaaa acttgaccca
    28741 aatcgcatat ggtggccaag ctccccctgc gccggtcctg atcgctttga aaacaactgg
    28801 acaaatgaca atatgggaga tatgcactac tgggaagtat ggcaccaaaa aaaatcattc
    28861 gacgcctacc tcgatatacg gccgagattt gtctcagagt ttggctatca atcatttcca
    28921 tctctttcaa cagtgcaaag ctatacgagc gaaaatccta taaatcttac acatcccgtt
    28981 atggaattcc accaaaggtc tccaggagga aactccatca tgatggaaaa ctttacacgt
    29041 tattttcgtt ttcccaaatc aacagaaaaa atgctctacc tcagtcaagt acaacaagcc
    29101 cttgctataa aaacagcctg cgagtattgg agaagtttaa aaccctactg catgggaagc
    29161 cttatttggc aattaaatga taactgccct gtagcttctt ggtcttctat cgattacaca
    29221 ggaaaatgga agatgctcca ccatgaagcc aaaaaattct tttctcccat tgcgctgagt
    29281 atgtacagta aggatgaaaa actttttatc tttggcgtaa acgatatcca aaaaaagctt
    29341 gacgcaaaac tctgcctttc gttttacaat ttttctggtg aaaaaataca agaagatata
    29401 caactaaatg taagtctcga agctgacgca gtaagtgaac ttttttcatc tgaaatcgaa
    29461 aaactaccga aggatatttc aagctatttt ctctatgcaa aacttgaagt agcgacagag
    29521 aagggaaaaa cacaaagcta tgaagcaaca cagtttcttg acttatacaa aaaactcgat
    29581 ataaaagaag ctgctataga ctatacaatt actgaaaaat cgattacaga acttccgtca
    29641 gatattctgc aaatgaagca ttcccgtaca agtcaatttt tccaaattga tatagaagta
    29701 aaagcacctg cattttttgt gagtttagat gcggcttcga ttaagggact cttttctgat
    29761 aatcttctta caattcttcc ccaagaaaag aaacgcctgt attttattcc ggagacaaaa
    29821 acaagtcttc aaaaattcaa gaaaatgctt aaagtcgaag acttgcgctc atcctactaa
    29881 aatcgtttaa agacaaaaaa agctgttagt gtcaaactaa cagctttttt ttattataca
    29941 agagctctat tctttaagcg ctccaccagc aacaccttct gcaagacgtt tttggaatat
    30001 aaagtaaacc aagataatcg gcaatgttgc aatcacaaga gctgcgattt gccaaccaag
    30061 ctgtgttccc gttctgcttg agaaagagta gactccaaca gggagggact tcgtaaactc
    30121 tttacttgcc ataactaaaa caagcaagaa ttcattccat attcccagtc cactcataat
    30181 acttatggta ataataacag gtacagtcat aggtacaata atagctataa atgttttgaa
    30241 agcagttgca ccgtcaatat aggcagattc aatcaagcta ttaggtagtc cacgaatata
    30301 ctgagacgta agcaataccg ccatcggaag cccaaatgcc gaatatacta aaataatacc
    30361 aaggtgcgta tcagttaagc ctattttcga tagcattaag aagaggggga ttataagttg
    30421 gttaatatca atcaaatacc ccaaacctat gagcccgact attaatgcgg caagcttttt
    30481 gaactttaac ttactgactg catagccaat cattaaacca aaaaggataa caaagaatgt
    30541 cgacacaaag gtataaataa ttgagttaag aaatttacca ccgaggcctc cacgaaccca
    30601 agcagaggta taattccaaa atacagtatc ccagtgaatc tgtctttggt attttttagg
    30661 aagctcatta acacgaagcc tgtcgccgtt ttttagcgca ttgatttcag aggacatttc
    30721 tgaacgatct aaaaaagcta ccttaacacg ccttccgggt gccatcgatg ttgattcaat
    30781 aataataaga tgctcattaa taatatcgta atcggaaact tcgaaaccaa atttttttat
    30841 aattttcttt ggatatacaa tgtttaattg cggttgcact acttcgtatt catcatcttt
    30901 gttaaaaaac atatcgtgtg gaagtgaata catacttata gtcaattctt catttgtctt
    30961 aaatgaagca tatcccatcc atactaaggg agatatagta agcaatgtcc aaaaaataaa
    31021 tataaaatag gaaagaaatt ttgtaaagta attaccaaga aagtttgttc tcaccatgtt
    31081 ttcattataa ctcattttaa tactcctcgt ccgttccaag ttttttgcca ataaagttgc
    31141 taaacataat taaaataata ctgatcaaca caacagtgtt tgaaatagca gcaccgtaag
    31201 caaaacgaag tgggtttgta aagtcattaa aggcaactcg gtacatcaat aatggtaaaa
    31261 cttcaacatt tagacgcgtt aagccatcag ttgtaatacc gaaaaggagg ttaaaacctt
    31321 ttagcgagcc agcgatagct aaaatagatg aaactaagat cgttcctgca aggaggggaa
    31381 caataatacg tataaagatt tgtgtttctg ttgctccatc tatttttgca gcttcaatca
    31441 tactatcatt aattttttgt aagttcgcaa gaaaaataat catataaaag ccggtatata
    31501 cccatataag cgcaatacct acaggtatca ttgctgtttt tggatccagc ataagggtaa
    31561 attgctttgt tggatcaccg ctcatcgctt gcataatacg tgcaacagga ccatcgacca
    31621 tgaataacat tttccacatt atacccacaa caatcattga caaaaactgt ggtaaaaaaa
    31681 ccattgattg gaaaaaattt gcagctttaa cttgctttct atataaaata taggcaagaa
    31741 taaaaccaat cggtatttgt ccaaagatcg aaacagcaac aacgatcatg ttgtttttaa
    31801 aagcaaacca aaacattctc tcaccaaaca ttttaatgta gtgagttaaa ccaacaaaac
    31861 cccattttcc accaatgttt gggttatagt ttgtaaagct taaaaaaact gaataaataa
    31921 tgggatacgc gacaatgaat aaatagagcg caagccctgg cataacaaag cctaaaaaag
    31981 tcgctaaatc tcttgtttta atttttttca caagattaac actcctatac taatttaaaa
    32041 gggatttaag gccacgattt ttttactaaa aaaccatgtt aacaaaccct tttgctcctc
    32101 cttgaatgaa gctctcaata aggattataa atctcttatc gaacaagcac atcaagcaag
    32161 gatattgtac tattacaaac gcaatttgta aagtgttttc acgttaaaac atggcctttt
    32221 ctaccaaaac agcctctttt tttattacat ctattctata aactattatc aagattttgt
    32281 gattctatat ctttaaaata ctttagagtt ccaaccttta gttcatctgt tgcatcttca
    32341 tcacaaacaa taatagcttt tttgtgcatt tgcaagcaag aaatagtcca catttggttt
    32401 atcccttctt caactgcatg ttttaaagcc cgtgccttac tgtgcccact tacaagtatc
    32461 ataacttcat cagaatcggt aatcgtacca acacctacgg taagcgaggt tgttggaact
    32521 tggcttatat caccaccgaa aaaacgtgag tttacttgaa ttgtttcgcg tgttaaggtt
    32581 tttactcgtg tccgtgagct aagggatgaa cctggctcat taaaagcgat gtgtccatct
    32641 acaccaactc cacccaagaa aagtttaata ccaccaaaag atgcaattgt tttttcatat
    32701 gcttcacatt ctgcatccaa gtcttgtgcc ataccattta atatatgtac attttctttt
    32761 ttaatattta cgtgactaaa aaagttgtcc cacataaatc tatgataact ttgtgggtga
    32821 tctgaaggaa gcccaacata ttcgtccatg ttgaatgtaa ca
//
LOCUS       DAOSSW010000010        32131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_1537_length_32131_cov_6.589631, whole genome shotgun sequence.
ACCESSION   DAOSSW010000010 DAOSSW010000000
VERSION     DAOSSW010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 32131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32131
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1537_length_32131_cov_6.589631"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..1017)
                     /locus_tag="PKW26_01845"
     CDS             complement(193..1017)
                     /locus_tag="PKW26_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ92345.1"
                     /translation="MQVLLAGTGTSHGMPVINCLCPRCTSKDPKDNRLRTSAFIRGKN
                     GESIVIDIGPDFRSQALRYGLPSLDAVFITHSHADHLHGLDDVRIFSHTCSFAQNRGA
                     DCDTLKVYADRLTIKDIKNRFSYVFKKTQEGGGKPRISLKNVEAYKEKPVKIGNINIL
                     PIPLEHGDIGTTGYRIEEDDFSIAYLTDCNGIPKSSLEKLKNLDFLIIDALRKRPHST
                     HFNFDEALEVAEILQPKQAYITHICHDYTYEEINAYFAKKSLFPIQSAYDGLILKK"
     gene            1099..1701
                     /locus_tag="PKW26_01850"
     CDS             1099..1701
                     /locus_tag="PKW26_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92346.1"
                     /translation="MSDMMSVLDYILNNCSAKELEVIETAVKKRKTNLGSASLDLNAF
                     AKNLSKTMNDSISQTISGFQESLKDFAWDLVKKENPDLSDEDTEIIVNQMIPDILASR
                     KELKVADYIRSGESLVHEGQVNGFPIDAMLEMVFQFISYSNGSLAPEEDRALHNAVKD
                     WPTVFWKAFPEPLQELIRSYLKGMIDSNVFENALTILLSE"
     gene            complement(1718..3706)
                     /gene="uvrB"
                     /locus_tag="PKW26_01855"
     CDS             complement(1718..3706)
                     /gene="uvrB"
                     /locus_tag="PKW26_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOQ92347.1"
                     /translation="MRAFVIDSPYDPAGDQPQAIEALSQGFLRGDRYQTLKGVTGSGK
                     TFTMAKIIEQVQKTTLIISHNKTLAAQLYREFKSFFPNNAVEYFVSYYDYYQPEAYVP
                     ARDLYIEKDASINDEIDRLRLAATYSLMERRDVIVIATVSCIYGLGMPEMYRDMRVHI
                     EKKQSLSPSTFAKNLINLQYERNEILDRGHFRMRGDVLEVFPAYMSDAYRIELDWEEV
                     SRIRRFNPLTGETIEELDEVIIYPAKHFVVPHEMLMKARDKIKAELDVQYQFLLQEGK
                     MLEAERLKTRTNYDLEMLAEMGYCSGIENYSAPIGNRPPGVPPATLLHYFPKDFLCFI
                     DESHVTVSQIGAMYEGDRSRKQNLIDFGFRLPSALDNRPLKIDEFEGMTNQVIYVSAT
                     PRQLEYDRSTQIVEQLIRPTGLLDPIVEVHPSEGQMKHIFGEVQKRIAANERSLVLTL
                     TKKMAEDLTDYLISHGLKVRYIHSEVETIERVEILKGLRAGEFDVLVGINLLREGIDL
                     PEVSFIGILDADKIGFLRSTTSLIQIIGRAARNQEGRVVMYADRMSDAMKEAIEETQR
                     RREIQEAFNKEHGITPTTISKAIEDILVRQIEEEKEVVETELSVLKNSFNMLIPKERK
                     KLIRALEKEMMDCAERLEFEKAALIRDEITKLKEEHDI"
     gene            complement(3723..4943)
                     /locus_tag="PKW26_01860"
     CDS             complement(3723..4943)
                     /locus_tag="PKW26_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOQ92348.1"
                     /translation="MKLYTKKHVIISSLVSASLALIFAFVFIFLGPSYKKEGQTIQTS
                     ENTNFINETNTPPFSTIQQSLVDYTAGYTIDEQQNILVYENFSPAVVNISTEVISYSW
                     FSQPYPTEGSSGSGSIIDARGYVVTNRHVVANAYKIYVSLSDGTQYEGKVIGVDAQTD
                     IAVIKFDPPKNISLKTISYADSNNLKVGQKVIAIGNPFGFDRTMTTGIISSLGRPIQI
                     DKNIIIKDMIQTDSAINPGNSGGPLLDSSGKMIGINTMIYSTSGSSAGVGFAVPVNTA
                     KRIVSDIMQYGYVNRGSLNGSFVELNATIANYANISIQKGLLVSELPKNSNLAKAGIK
                     AGNEAVRYNRFSSIFYIGGDIITEINGAEISSLVDLNTALEESRPGDLVQVGIQRGKQ
                     KLTIPVSLSETTTK"
     gene            complement(5039..6805)
                     /locus_tag="PKW26_01865"
     CDS             complement(5039..6805)
                     /locus_tag="PKW26_01865"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOQ92349.1"
                     /translation="MAKISALQNARYAYKPKLPAVLKLRLNTIQVAFGKPTKAISDEA
                     ALKELFKNTYGKPIAHFVKGKNPAVKELIRVGVILSGGQAPGGHNVIAGLYDGIKAGN
                     PDSKLYGFLNGPAGLIDAKYVEIKDRFMDEYRNTGGFDIIGSGRTKIETPEQIASSME
                     TAKKLKLDAIVIIGGDDSNTNAALLAEHFIQTGMSTRVIGVPKTIDGDLKNKQIETSF
                     GFDTATKTYSELIGNIGRDANSAKKYWHFIKLMGRSASHIALECALQTQPNICLISEE
                     IEEKKMTLKQISDDICTVIAKRAENDENFGIVLIPEGLVEFIPEMKKLIAELNDKMAT
                     EANSFEKLKSFADKKDWLKSHLTASSFKTFDSLPEAIADQFLADRDPHGNVQVSRIDT
                     EKLLVSMVETQLAEMKKKGSYTGTFSSYTHFFGYEGRCAFPSNFDSDYCYALGYTAFV
                     LIASGLTGYLSSVQNLTADAKDWTAGGIPLTMMMNMEQRHGSQKPVIKKALVELDGKP
                     FKTFAKNRDKWALKTSYLFPGAIQYYGPSEVCDTTTQTLLLERGKGKPTEVVVADAPK
                     ASAKASAKKTTAKTKPAAKTKK"
     gene            complement(6913..7821)
                     /locus_tag="PKW26_01870"
     CDS             complement(6913..7821)
                     /locus_tag="PKW26_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOQ92350.1"
                     /translation="MLDKKITKWLRQGLGVFLFLAFSFYFPLYAEEKSFEKQHPFFEI
                     SFLEIPLCPAIAEEKTEDEDSTIEFFEKKQDHELRVFEYYQLCYRESFEQCEWAAYTI
                     TTEKLEKNTSRKDNFRVDPKISTGSASLEDYRGSGYDRGHIAPAADFAFDSLAMSETF
                     YMSNMSPQVPAMNRGIWANLEAEGRKWAERYGIVYVVTGPLLENKAYKTIGINKVAVP
                     DAYYKIFLSPIVKRNRETDDLFFAAIAAAYIIPNAKGEKHFSEYLCTIREVEKITGID
                     FFYKLEDEIENKIETESLQEVFIETE"
     gene            complement(7826..8413)
                     /locus_tag="PKW26_01875"
     CDS             complement(7826..8413)
                     /locus_tag="PKW26_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92351.1"
                     /translation="MEDNKINLLALIPHGNEIAVLKKIQARLVNEKVYPMHPLFCPLV
                     LFNTKQTIKEQKEILESMKNDFQKKKNPVQLREIERHGQWLSLAFNISFEIKKHRTEI
                     QIADFPPLPNTISMPLVFVKKNEDSQTVFSSNFFNDVTERKKEIHSVEDLQKNLPSEI
                     RVFKLALLEFSWDRKWEYAFHWEELDAKWVKIKQG"
     gene            8480..10264
                     /locus_tag="PKW26_01880"
     CDS             8480..10264
                     /locus_tag="PKW26_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92352.1"
                     /translation="MKKVFVFIITVLLSIMSLASCNSVSKSSIALELEDLSFSLFAHN
                     ANAKKAFIPSQGKSPYMFFSFDDSFHLFVDEYKDTSLALCVDIDFAKDRTDVKNRGKE
                     NKIAFGFLTKDDFTSDGLLKTGTLVSRSMVTGFIDSADFQEGMSLSFSLPQDTKIKGF
                     FLYATEDLYLKKARIQKPSLGWSFAEERAFFAFSAMGGEIPSSLAQAQDEGLSLALSS
                     SLELDTKESYLSLAFSPQEQLSHPERYPSVVLDLDKESFTIQRSPLQTKAHIYASQML
                     SPTAQVFCSQNKEMLSELLFMSNEGIRKKTSTEKSLEPLHTDPGLIIIWDKASWRQES
                     YELFSWELFPSVLIMDFKNYAIQDDYLKRLAFFAEKKGYTGRLSYDDEIKSMHGFNAH
                     DYSAETLANFFALAEKENFPLNQSELHLRSILLENGVIRFENGKLVEGAGALLSISQE
                     STLALRYTLMGHEAYHGIYFTEKLFRDHTLEVFNASDPNSRDFLMGYFTTSETLNYDI
                     ENDYLLKNEFMAYLLQQSIANTGQYFVNIASRPSVIKNLPELSLYVRNSRGSGFTEAC
                     RELDAFIYRSYGLAAGRVRLVGRTRSSF"
     gene            complement(10287..10880)
                     /locus_tag="PKW26_01885"
     CDS             complement(10287..10880)
                     /locus_tag="PKW26_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ92353.1"
                     /translation="MKVYFNLSLEGFSNCYVVTNPEEKAAIIIDPGTVTKEILLQIER
                     EQLNLEAVLITHNHESHLKGLATLRKIYTPKIYAADYEVADRETQILKDDGILRIGGL
                     QVGYMSVPGHTPDSMVFKIGKVLFTGDALTASTLGSTTNTYAERMLLAAVHNKILSQH
                     DDTVIMPGHGPPSSVAAEKKFNTSVVVRTVSGLDDKG"
     gene            complement(10864..11502)
                     /locus_tag="PKW26_01890"
     CDS             complement(10864..11502)
                     /locus_tag="PKW26_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOQ92354.1"
                     /translation="MFSYVICQHPSCQKTAVSNYSKDTKTLSTHEYCYEHSPIASQIE
                     EYIYEYIKSNDKIVGLNASGISFQNLDFTNKRFYGCNFYGCTFRNIHSENLRSRMSMF
                     DFSIFSDCNLLGSNLQFTSFAGATFSHILFTNSDLVHNNFCGSHILQSSFDDSDLYNS
                     RFIRARLQNTSFRNCNIKKTMFFEMSQENVSFKLSNTREALFSKEIDYESLF"
     gene            11556..12365
                     /gene="murI"
                     /locus_tag="PKW26_01895"
     CDS             11556..12365
                     /gene="murI"
                     /locus_tag="PKW26_01895"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOQ92355.1"
                     /translation="MQKNKTCTDFLCIDSGSGGLPYLLYLLERFPGYSSVYVADTENF
                     PYGKKSKEKIVEAITALVERLLEHFEPKIIIIACNTISVTALDILRSRFSIPFVGTVP
                     AIKQAVQLSKNKIIGLLATENTVASPYTDALFLEYASEFRLIKRGDAKLVRFVEKSLH
                     KADEKKRKKALKPALAFFEKNNIDVLILGCTHFIHLINEIKNSAPSHWQILDSRAGVI
                     NRAIHIANEKGIVPRLESKALFYATKQYSASEEILLRSFLEQNAISWQGLI"
     gene            12461..12937
                     /locus_tag="PKW26_01900"
     CDS             12461..12937
                     /locus_tag="PKW26_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92356.1"
                     /translation="MARKVSLGVFFLQIALALFLLFSGLNATSAVVKSDSFGFSATIS
                     RNFGDNEVVTIVDQLLPKNKSLATGIIIILAIVQIACGAILLLNFFIETKQITDILLI
                     IMLVVWALIIIFLDIIGTGGLINGAFKNYKTFVAFCKQLSQHLLVIGAILLAFKNE"
     gene            complement(13016..13089)
                     /locus_tag="PKW26_01905"
     tRNA            complement(13016..13089)
                     /locus_tag="PKW26_01905"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13053..13055),aa:Arg,seq:cct)
     gene            13157..14620
                     /locus_tag="PKW26_01910"
     CDS             13157..14620
                     /locus_tag="PKW26_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708027.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOQ92357.1"
                     /translation="MASFVLLLLFLFVMIAVGIWSIRKTTTLNDFFLGGRNLGPWVSA
                     FAYGTTYFSAVIFIGFAGKQGWSFGLNALWIGIGNAILGAFLAWILFARRTRRMTQNL
                     NTMTMPEFLEARYESDHMKMFAALLIFVFLLPYSASVFKGLGHLFEMTFHISFDFALI
                     ILIAITGIYLVLGGYFAVTITDFIQGFIMLTGAIGMVIVLVKNAGGLLPAIQSIQENY
                     VQHVPVGERPSWLTIASLIFMTSFGTWGLPQMVQKFYALKNEGIIKKAALITTVFALV
                     IGVSAYMTGALSHVFFSPESLPRLESGAINFDMIVPILLSTHLPELLLAIIMLLVLSA
                     SMSTLASIILVSSSAIVIDLYKGYLHKEVSDSHSVLLMRVLSAVFILFSYIISKMQLG
                     FIVTLMSLSWGVLSGAFMAPYILGIFSKKITKAGSYAGLYSGAGTAIILNLVLAAQNS
                     PLASSIAMIVPFLVVPIVSAFTQKPSQELLEKAFTGI"
     gene            complement(14617..16404)
                     /locus_tag="PKW26_01915"
     CDS             complement(14617..16404)
                     /locus_tag="PKW26_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOQ92358.1"
                     /translation="MITEILLQQKTMLIVDDFEMNRDILQNIFKDEFHILQAENGIEA
                     MKLLHEKKTEIDIILLDLVMPEKDGFSVLKEVRAHEELSNIPIVVVSASGEIDGELLS
                     LELGATDFIVKPIKPHIARLRVQNILTRKEVDKLRIQHTMMLEQHQKERVLLNLITQS
                     KNEYQKLAEYDQLTGIYNKTMFVQETKKMLQKNPDTTYVLVRFDIEKFKVINEFLGHQ
                     EGDALLKHIASILKKYGSNLGTYGRIASDIFVACFPHSKENVEKLVQTYTDSMKLYNL
                     NLEIILCFGLYIITDTDVPIDSMIDRANLALQTIKGNYITRFAYYDEALRQILIEEQE
                     IVNEMEDALEKGQFKMYLQPKCDLKDGKIVGAEALVRWAHPKKGIIPPGKFIPIFEKN
                     GFVMALDQFIWEEACKLLKKWIDEGSPVLPISVNISRVNLFNPQLASIIKNLAEKYGI
                     PPRLLELEITESAYTNNAYLLVDLIHELHENGFTVLMDDFGSGYSSLNMLKEIPVDAL
                     KIDLHFLSGSDILGRGEQILESIVNMSHAINLPTIVEGVETKDQVDFLQSIRCNRAQG
                     YYFYRPMPVEEFEKTLHQKTTKKSNSLEN"
     gene            complement(16757..16829)
                     /locus_tag="PKW26_01920"
     tRNA            complement(16757..16829)
                     /locus_tag="PKW26_01920"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16794..16796),aa:Lys,seq:ttt)
     gene            complement(16900..18060)
                     /locus_tag="PKW26_01925"
     CDS             complement(16900..18060)
                     /locus_tag="PKW26_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92359.1"
                     /translation="MKKYNILAILLVITSFALIAQEFTYELAMPEIRHARIDALGGKH
                     ISDTSWYYSFLTNPGNIGLMGKKSLYPGLTIGLGGPIKYLSDIITIANDMENSESINS
                     LAKILSENKGLDLDVVISGPIQFGSVKNNFGWGFFNQTYVIANAPSISYLEGLVGNES
                     LLRFGYALPIPLGIGKLSVGGAVDLVNRVEGKILTSVTNLMSGNSEEMPLPLYTSFGF
                     GLDLGATLSLLDFITVGLVWDDAVSGYFTSKSPNLASGELEPDASYQAFNFDWKFNLG
                     ASVSVPSVNVFTLGVISSLNVMLDLHDVIGMMVNDPKKRNPILDVSLGTEAVLFKTIS
                     LRLGLHEMYPSAGIGARFGAFNLDAAIFGRELGLEPGTRPQLNLAISLEFFK"
     gene            complement(18047..18763)
                     /locus_tag="PKW26_01930"
     CDS             complement(18047..18763)
                     /locus_tag="PKW26_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92360.1"
                     /translation="MKKIIIGVFSVFLLFSFISCDFFVTSWGSGMQRDLSTALKKESA
                     GDLAKLAKDPQFQDPSSGVAVLNALSGKSEADIQALSVSEQESIMDLALAVSIPTSVV
                     TELADIAQDPDNAEPEELFNTILGGINSFDTTVLQAILNDEDALESASPESLVSASLA
                     LVAQVMKREGENLNFDDLADLFNDDNDGVDISPESKADIEAVKAVLDLFDEGGKRSDE
                     ELTLFGLSLEDYLKVKNEKI"
     gene            complement(18815..20221)
                     /gene="mtaB"
                     /locus_tag="PKW26_01935"
     CDS             complement(18815..20221)
                     /gene="mtaB"
                     /locus_tag="PKW26_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOQ92361.1"
                     /translation="MTIHFETLGCKLNQIESESAAKAFYDQGYIISSKNLTRASGTQK
                     DIGLAIINTCTVTGKAEQKARRLINMLLDLCPDAPIIVTGCYAELDGAYLEEIDNRVI
                     ILPGSKKGYLTDLAEAFPASSLQKKFHLESLKEWIDTKTREEENLAFRLSTDVFFSHS
                     RASLKIQDGCNSACSYCRVRLARGKAISLDAETICDRIRELIEKGHKEVVLTGINLSQ
                     YFCPKTKMGFTALLDYLIKNTEGIAFRISSLYPQSVDEAFCELIQSERIRPHFHLSVQ
                     SGSDKILKAMKRPYNAKKVSEAVHLIRKAKEDVFIATDIIVGFPGETEEDFLETLSLC
                     KELNFAWIHVFPFSARPGTEAFLLKNTISAEILQKRVILMTELAKEQKKAFVLSQVGK
                     KRLAIVEKRRSIPARAVCDNFLHVELSNLNTKEAQMLAGQEIRILVERYESSNKIDAR
                     ACVFSESDVQLSRLQEIT"
     gene            complement(20272..20895)
                     /locus_tag="PKW26_01940"
     CDS             complement(20272..20895)
                     /locus_tag="PKW26_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92362.1"
                     /translation="MKKNVGFVLLLLLSVSFVITQTKPDALKEYNAGNYIRAIEICED
                     EIHVNDKNMDSYAVLCWSLIANKQYNEAEFWANHARKIAQYDQRIIEALAEAKFYLGK
                     NQEALSLFQEYLSLVSLSASRAGLAYYFMGEIFIRQAKYQHADIALSQAVKIEPLKDF
                     WWIRLGYTRELARSYGSALLAYDKALSLSPFLEDAINGRERVKKLIR"
     gene            complement(20904..21269)
                     /locus_tag="PKW26_01945"
     CDS             complement(20904..21269)
                     /locus_tag="PKW26_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOQ92363.1"
                     /translation="MLKGKDSELLELEEEDFDTILASDISFAGTIRFSKPFMIRGTVT
                     GLIEATSDLVIDSDASTFADIVAERVLVRGKVEGNIKAKRLVFVASTGVVTGDIFAEQ
                     VVLEPGSTFTGRCVMTNQK"
     gene            21394..22404
                     /gene="pta"
                     /locus_tag="PKW26_01950"
     CDS             21394..22404
                     /gene="pta"
                     /locus_tag="PKW26_01950"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOQ92364.1"
                     /translation="MDFVQKMKTQAQMLQNRLVLPEGSEERVIFAAEKIMREKLAKEV
                     FLIGEEKKIKEFAKEKSISLDGITILDPNKSKFFDDFCTTYVELRKHKGMTLEQARID
                     MKENNRFAAMMVRKGKADSMVAGAISTTADVLRAGLTIIGTAEGTKTASSCFVMQMKD
                     SFWGAEGLMIFSDCAVIPKPTAQQLSDIAISAGQSCKSLIGSDPVVAMLSFSTKGSGG
                     KDEDVLRVQEAVKLAKEKDPSLLIDGELQADAALVPSITQKKAPDSPITGKVNTLVFP
                     DLGSGNISYKLVQRLAGAEAFGPFLQGFAQPISDLSRGCSVEDIIAVSAVTLVQAGKK
                     EA"
     gene            22410..24353
                     /locus_tag="PKW26_01955"
     CDS             22410..24353
                     /locus_tag="PKW26_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92365.1"
                     /translation="MEERTKVLESLFKQAVREGQKRNYKKATALLESILLQYPQKQSQ
                     GYEKDFKCKVLLYLSRSYHAMGDYAMAISNLNSYVNLAKKDPDGWFFLGRSYLALSSY
                     SVAIQCLKKSLEFRPKSDTTLALLGTAYFKSGKTNLAVESFKEALYINPDDAQINQAY
                     QNSLFVEAIKIYKRGDIELAKRMFAFCINNGMDSVLPRLYYAHCLREQKHLEAALDAY
                     KEAIALAPDDPSLRWYELSILSMMGKTEEARRLVESLSVEFDDFDFSLEKGKDQKAQS
                     LEIIQKALAEENWKEVIEAGRLYIQTFGSDAIIHSFMGEASRNLSRLEEAENHFQKAK
                     RLDPQYRAYRYGLLITYLSMENWEALDAELAKNNSQSPLDDETIAYYSVICDAKLKKE
                     PKTVLEAVQKIFRKNPADTYMMGVLAEQYIRVGLPELSQPWLQKLLDLGIANESQYLL
                     LIECAEQLKNKKDFESACLRYLEGWQANIDIRKKLIRFYSRQEKWKQAANHIETLLAY
                     SKHQETLTKNLAVFRFKARQFHHAAILYRALLQEKPDDREAMQRYVFCLYKLDMIPQA
                     IRFLQLWHKSYSVDVDGILIEAILHLHNEESEKAIQLLRSSMEKFKNDKRIPKKLASI
                     YQNQGNIDMARQFDPSISYTKIQ"
     gene            24494..25474
                     /locus_tag="PKW26_01960"
     CDS             24494..25474
                     /locus_tag="PKW26_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92366.1"
                     /translation="MGFKAMDLYDAYNKNMLPKEEGYVVSAYFVNGSPYSIYEIISYA
                     GVKNFYASGDSITFQSDGKKIYILVEPTSYTLKAQEPYVRPQSYQIPMRFNELEIYTC
                     RNQYKLMYSKEAQMVMTSFTVMKPTGSNFSFIIFPKDDIEQTITLLFQKAFNGQSNVP
                     LSDAKKVATSLAQKIMKELSWPNKEKNAAKKKATEKAKPGPKPKAAAKPAEKAKPGPK
                     PKAAAKPAEKAKPGPKPKVAAKPAEKAKPGPKPKAAAKPAEKAKPGPKPKAAAKPAEK
                     AKPGPKPKAAAKPAEKAKPGPKPKAATKAAEKAKPGPKPKATAKTIVKKK"
     gene            complement(25521..27272)
                     /locus_tag="PKW26_01965"
     CDS             complement(25521..27272)
                     /locus_tag="PKW26_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ92367.1"
                     /translation="MGGKQQLIKKNLPYIKVLPQWAQELSYKYCSKTVNMYLVHGNIR
                     DFLPHSMNEGEFSFKKIQEYISEVLFGNENIVVYYDRSSGVSFCTLEMEKEYLATMKH
                     FFPKLSDTELLSKDPRLAFEYLEKYFLINIPRNKRIVFLIDYAETIIPYENIGLLDDT
                     DRFCFVTLNRWSHEPFFTQGDVSIIVLTENVSDINLRISSSPSVVKVHIPLPDAFLRK
                     HFLDYLLSQDLLLLERGMTSEKMSALSAGLNLWHLHQIATESFQEDVPIDLSYLRQKK
                     REILEKEGAGLIEFIDANHDLSTVSGHDFVIGRLKNAARAIKQNQLDVLPMGYLISGP
                     IGTGKSFLVQAFAGEIGIPMVRLCNFSSQANGIVETNLERVFNILKALSPVAVMIDEA
                     DTLLGRRDDNRGDGSNSRIFGQIASFMGNTAYRGKILWFLITSRPDLIPIDLKRQGRA
                     EEHLALFYPDSVEEKIALFEALKKKLSIKVEQDSFHDVFKKINFEISGADIEAILVRS
                     KMHAAMDKRIIVTKADLEHTIRDFIPPSYPHEIALQNLVAVLECTSKQMVPKRFQNLD
                     RGKLAQEIRDLKQLLQI"
     gene            27386..27559
                     /locus_tag="PKW26_01970"
     CDS             27386..27559
                     /locus_tag="PKW26_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOQ92368.1"
                     /translation="MAYVISPDLCNNCGTCEPECPTEAISEQNDKRFIDADLCISCGA
                     CAAVCPTEAISEE"
     gene            27849..28775
                     /locus_tag="PKW26_01975"
     CDS             27849..28775
                     /locus_tag="PKW26_01975"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ92369.1"
                     /translation="MGYINVYKKNNSILCFFFFILLHTRFISAQKLPEISDLSHKNIL
                     FKQFSEEVEKNYRLLAQGNTSISLQFFYYRAEKNDTLLTIAARSLIPYETLASLNRLG
                     EVGANIEGKLLILPTCPGLFILEKPFSPLEILLKEKYLHEPNLVCYTLGDTLFYFIQD
                     ARLSGTERAFFLDSNLRLPVFHAVLSSNYGQRLSPITGKSSFHEGVDLAAPEGSLVMA
                     CKAGQIEQIGYSQIYGNFIIIKHEDEKGRQMRSFYAHLKTIDCKEASFVLGGATIGSV
                     GSTGLSTGPHLHFEIWLNGKTIDPNELIKGIR"
     gene            28775..29809
                     /locus_tag="PKW26_01980"
     CDS             28775..29809
                     /locus_tag="PKW26_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92370.1"
                     /translation="MKKKGLIVLFFFLTLFCFTKDMFRVHETQLVEMQDEQQSLKARL
                     AINDMLVIKLPKTIYFLQGLSLEIKIPKAVADYRDAVAFYVYKNMSPMPTPKTIDYAG
                     DRVFLNTFPGRLSYNFQIPLAKNHTLKESPYSALLPEVIDVNEGYVYFRLQLVMKGTP
                     VAVLESEFDIEVKPILIDKGMLNLSLIPPNAHTRQQIPGEDLKADKDIDLVANKKNYA
                     LFIDEKPVDMIDNTILLNSGVHHLAVLSDYFRNEVRSFSIQQGKTTNLTIQLETNEPT
                     MQILAPESLEVYFNDVLLTDFSKPYQITEGEHKLRFLLDGYEFIKSFTALRGKSYSIT
                     TNIDFFIEER"
     gene            30058..32103
                     /locus_tag="PKW26_01985"
     CDS             30058..32103
                     /locus_tag="PKW26_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOQ92371.1"
                     /translation="MATIKKRTIFYFLFTLFFALLFGLSLGLLLANTANTIHTENFYE
                     LEIALPTRLLDINGEFITEFASAEKRELVDYIDLPKHMIDALISREDKDFFQHSGFKV
                     KAIVRAVLGKLLRKNWGGGSTLTQQIAGTVYADRSDMSYRRKLRELFWTFQIERRYAK
                     EEILELYLNKIVFGNGTYGVSAASKYYFGHGPDKITPAEAAILVSQLSAPVYNNPFEY
                     PARAKVRQEDVLNKMVKYGFLSQEIAAASLEDYWAHFDYTRTSVGAYFLREDKAPWFS
                     AHVLSELINMMYGSNVDVYTSGYTVNTTMNLKHQLAAQRIMDERIRFANRENQRTASA
                     SSSVAVNTYIPMTELISLVFNLPSLKVSEQRSERLALSAYKNEINPILDVASLLFGME
                     DLKISVVNKANVEKSKDVSKDSIEGCLITIENETGHITALIGGSQYSTENQFNRATQG
                     LLQPGSTFKPLYYSAAIDSRKLTAASKIADVPTLFYNESGVPYYPENFRGEWMGTVQL
                     WYALAKSMNVPSLKVMEAVGFDAAIKRSTALLGIPESQYKTRDFSRVYPLGLGTAIVK
                     PIELARAYAVFGNQGREVTPISILSVEDKKGKIIANPEQDLRAEQKKKGSAIQVISPQ
                     NAFIMTDILSNSVLMGTMAYGSSNGKKLEYRRSDGTRYTIPAAGKTGTTQNWSDAWN"
BASE COUNT         9324 a         6190 c         6402 g        10215 t
ORIGIN      
        1 tgcacaaaat tatcgagcaa ggttgaaaag accttttctt tcggtatttt ttctaaattc
       61 ccaggttcga aagctgggtg cccaaataaa agaatttttt tatataactc atcattaaac
      121 ttttttgata tcataaaaac ctctaaactt ttaatttact ccattgtaaa gcggaacagt
      181 atttatagca agctattttt ttaaaatgag gccatcatag gccgattgta tcgggaaaag
      241 agactttttt gcaaaatacg cattaatttc ttcataagta tagtcgtggc aaatatgcgt
      301 tatataggct tgctttggtt ggagtatttc tgctacttcc aaggcttcat caaagttaaa
      361 gtgtgttgaa tggggtcttt tacggagtgc atcgattatg agaaaatcga gattttttag
      421 cttttcaaga gaagattttg gtatgccatt gcagtctgtt agataggcta tagaaaaatc
      481 atcttcttct attctgtatc ctgttgtacc aatatcgcca tgttcaagag gaataggaag
      541 aatgttaata tttcctatct tgactggttt ttctttatag gcttcgacat tttttaagct
      601 aatacgtggt tttccgccac cttcttgtgt ttttttaaat acataggaaa aacgattttt
      661 tatatctttt atagtaagtc tatcggcgta gacttttaat gtatcgcaat ctgcacctcg
      721 gttttgtgca aaagaacagg tatgggaaaa aatacgtaca tcatcgagac catgaagatg
      781 atcggcatgg ctatgagtga taaaaaccgc atcaagggaa ggaagcccgt agcgaagcgc
      841 ttgtgatcga aagtcgggtc caatgtcaat aacaatgctc tcaccatttt tcccgcgaat
      901 aaaggcggag gttctcagtc gattatcttt tgggtctttt gaagtgcagc gcggacagag
      961 acaattgatt accggcatgc catggctcgt tcctgttcct gctaataaaa cttgcatgac
     1021 aaaaataata aactttgtta tagtataggt caagccattt ttgaaaagag ttttttcaaa
     1081 atgccaaaaa ggaaagctat gtccgatatg atgtctgttt tagattatat tttaaataat
     1141 tgttctgcaa aggaacttga ggttatagag actgcggtaa aaaaaagaaa gactaatctt
     1201 gggtcggctt ctttggactt aaatgccttt gcaaaaaatc tttcaaaaac aatgaatgat
     1261 tctatttcac aaacaatttc tggtttccaa gaaagcctca aagattttgc ttgggatttg
     1321 gtaaaaaaag agaatcctga tttaagcgat gaagatacag aaattatagt taatcaaatg
     1381 attcccgata ttttagcatc tcgaaaagaa ttaaaggttg ctgattatat tcgaagtggt
     1441 gagagtttag ttcatgaggg gcaggtgaat ggcttcccaa tcgatgccat gctcgaaatg
     1501 gtgtttcaat ttattagtta cagtaatggg agtctcgctc cagaagaaga tcgggctttg
     1561 cataatgcgg taaaagattg gccgacagtt ttttggaaag cctttcccga acccttgcaa
     1621 gaattgattc gctcatactt aaaaggtatg attgattcaa atgtctttga aaatgcgctt
     1681 actatccttt tgtctgagta atttttagaa gccttgttta aatatcgtgt tcttctttaa
     1741 gctttgttat ttcatctcgt atgagggcag ctttttcaaa ttcaagccgt tcagcgcaat
     1801 ccatcatttc tttttccaag gcgcgtatga gtttttttcg ctcctttggt atgagcatat
     1861 tgaaagagtt tttcaaaaca gaaagctctg tttcaaccac ttctttttct tcttcaatct
     1921 gtcgtaccaa gatatcttca attgccttgg agatagttgt gggtgtgatg ccgtgctctt
     1981 tgttaaaggc ttcttgaatt tctcggcgcc tttgcgtttc ttcgattgct tctttcatag
     2041 catcgctcat gcggtcggca tacattacaa cgcggccttc ttgatttcgt gccgccctcc
     2101 caataatttg aatgagactt gttgtcgagc gtaaaaaacc aatcttatct gcatctaaga
     2161 tgccgataaa agaaacttcg ggtaaatcta taccttctcg aagtaagtta ataccgacaa
     2221 gcacgtcaaa ttcgcctgct cgtaagccct tgagaatttc tacccgttca attgtttcta
     2281 cttcactgtg aatatagcgt accttcaaac catggctgat taaataatcg gtaaggtctt
     2341 ctgccatttt ttttgtgagg gtaaggacaa ggctgcgttc atttgctgca attcgttttt
     2401 gtacttctcc aaagatatgt ttcatttgtc cttcactggg atgcacttcg acgattggat
     2461 cgagcagtcc ggttggtcga atgagttgtt cgacaatttg cgtgctgcga tcgtattcga
     2521 gttggcgagg ggttgcggag acatagataa cttgatttgt catgccttca aattcgtcta
     2581 tttttaaggg acggttatcg agggcactgg gaaggcgaaa tccaaagtca atgagatttt
     2641 gttttcgtga tcggtcgccc tcatacatgg cacctatttg gcttacggtt acgtgagact
     2701 cgtcgataaa gcatagaaaa tctttgggaa aataatgcaa aagggttgcc ggaggaacgc
     2761 cagggggacg atttccgatg ggtgcagaat aattttctat gcctgaacaa tagcccatct
     2821 ccgcaagcat ttccaagtcg tagtttgtcc ttgtttttaa acgctcagct tctagcatct
     2881 ttccttcttg caataaaaac tgatactgca catcgagctc agcttttatt ttatctcgcg
     2941 ccttcatgag catttcatgg ggaaccacaa agtgctttgc tgggtagatt atcacttcat
     3001 cgagttcttc gattgtttct ccagtaaggg gattgaatcg ccgaatcctg cttacttctt
     3061 cccagtcgag ttcaatgcga taggcgtcgc tcatataggc agggaaaact tcgaggacgt
     3121 ctcctcgcat gcgaaagtga ccgcggtcca aaatttcatt tcgctcgtat tgtaaattga
     3181 taaggttttt tgcaaaggtg gaggggctta ggctttgttt tttttctata tgcactcgca
     3241 tatcgcgata catttcgggc atacccaaac catagatgca ggaaacggtt gcgattacga
     3301 tgacgtctcg cctttccatg aggctgtagg tagcagcgag tctgaggcgg tctatttcgt
     3361 cgtttatgga ggcgtccttt tctatgtaga gatcgcgcgc agggacgtag gcttctggtt
     3421 gataataatc ataataggag acaaagtatt cgacagcatt gtttggaaaa aaacttttaa
     3481 attcacgata gagttgtgct gcgagcgttt tattgtggct aataataagc gttgtttttt
     3541 gaacctgctc gataattttt gccatggtaa aggtttttcc tgaaccggta acacccttta
     3601 gtgtttggta tctatcaccg cgtagaaaac cttgactcaa ggcttctatt gcttgcggtt
     3661 ggtctcctgc aggatcgtaa ggcgaatcaa ttacaaaggc tctcatcgtt cttagcctcc
     3721 tgttatttcg ttgttgtttc agaaaggctt acaggaatcg tgagtttttg cttacccctt
     3781 tgaatgccca cttgtacaag atcacctggt ctgctttcct cgagggctgt gtttaagtca
     3841 acgagggaag agatttctgc gccatttatt tcggttataa tatctccccc gatataaaag
     3901 atagaagaaa aacggttata gcgaactgcc tcatttcccg cctttatccc tgccttggca
     3961 aggttgctat tttttggaag ctcagaaaca agcaagccct tttgtataga aatatttgca
     4021 tagtttgcta ttgtggcgtt gagctcgaca aagctgccgt taagactgcc acggtttaca
     4081 tagccgtatt gcataatgtc agagacaatg cgtttggcgg tatttactgg aaccgcaaaa
     4141 ccaactcctg cagaacttcc agaagtcgaa taaatcattg tgtttatacc gatcattttt
     4201 ccacttgagt cgaggagggg accgccagaa tttccagggt ttatcgcaga gtcggtttgt
     4261 atcatgtctt ttattatgat gtttttatcg atttgaatcg ggcgaccgag gcttgaaata
     4321 atgcccgttg tcatcgttcg atcaaagcca aagggatttc ctatggcgat aactttttgt
     4381 cctaccttaa ggttatttga atccgcatag ctgatagttt tgagactgat attctttggt
     4441 gggtcaaatt ttataactgc aatatctgtt tgtgcatcga cacctattac cttgccttcg
     4501 tactgcgtac cgtcagaaag ggaaacataa attttatacg catttgcaac aacgtggcga
     4561 tttgtgacga catatcccct tgcatcgata atggaacctg acccacttga accttctgtt
     4621 ggatagggtt ggctaaacca actatagctt ataacttcgg tgcttatatt tacaactgcg
     4681 gggctaaagt tttcgtatac taaaatattt tgctgctcgt caatagtata gcctgctgta
     4741 taatcaacaa gactttgctg gatggtagaa aaaggagggg tgtttgtttc gtttataaag
     4801 tttgtatttt cgcttgtttg tattgtttgc ccctcttttt tatagcttgg accaaggaag
     4861 ataaagacaa aggcaaaaat taaggcaagg cttgcggaaa caagagagct tataataacg
     4921 tgtttttttg tatagagttt catagtacct tcctttataa gataaatata gcaaaaaaaa
     4981 acggcagagt caaaaggaaa gactaagccg ttttcatttt tttatacatt taaaaaaatt
     5041 atttttttgt ctttgcagcg ggttttgttt ttgctgtggt tttttttgca ctagcttttg
     5101 cagaagcttt tggcgcatcg gcaactacta cctcagttgg ctttcctttt ccacgttcga
     5161 gtaaaagtgt ttgtgttgtg gtatcgcaaa cttcacttgg accatagtac tggatagcac
     5221 cggggaagag gtaacttgtt ttaagtgccc atttgtcacg attttttgca aatgttttaa
     5281 atggcttgcc atcgagttca acgagggctt tttttataac aggtttttga cttccgtgtc
     5341 tttgctccat attcatcatc atggtcagtg gaataccacc tgctgtccag tcttttgcat
     5401 ctgcggtgag attttgcaca gatgagagat aacccgttaa accacttgca atgagcacaa
     5461 aggcggtata gccgagtgca tagcaataat ctgaatcgaa attgcttggg aaggcacaac
     5521 gaccttcata gccaaagaag tgtgtgtatg agctaaaggt tcctgtgtaa gaaccttttt
     5581 tcttcatttc tgcaagttgt gtttcaacca tactcactaa aagtttttca gtatcaatgc
     5641 gtgatacttg cacatttccg tgtgggtcac ggtcagccaa aaactggtct gcaattgctt
     5701 caggaagaga gtcaaaggtt ttgaaagagc ttgcggtaag atgtgacttg agccaatctt
     5761 ttttatcagc aaagctttta agtttttcaa aagaatttgc ttctgttgcc attttatcgt
     5821 ttaactccgc aatgagtttt ttcatttcgg ggataaattc aactagacct tctgggataa
     5881 gcacaatacc aaagttttca tcgttttcag ctcgttttgc aatgaccgta cagatatcat
     5941 cactgatttg ttttaaggtc attttttttt cttcaatttc ttcagagata agacaaatat
     6001 ttggctgtgt ttgcaatgcg cattccagtg cgatatgact agcagagcgt cccatgagtt
     6061 ttataaagtg ccagtatttt tttgcagagt ttgcatcgcg gccgatgttt ccgataagtt
     6121 ctgagtaggt ttttgttgcg gtgtcaaaac caaagcttgt ttcaatttgc ttgtttttta
     6181 agtcgccatc gattgttttt ggaacaccaa taacgcgtgt gctcatgccg gtttgtatga
     6241 agtgctcggc aaggagtgct gcgtttgtgt ttgagtcgtc tccaccgata ataacgatag
     6301 catcgagttt gagttttttt gcggtttcca tagaagacgc aatttgctca ggcgtttcga
     6361 tctttgttcg accagaaccg ataatatcaa aaccgcctgt atttcggtac tcgtccataa
     6421 atctgtcttt tatttcgaca tactttgcat cgattaaacc tgcaggaccg ttaaggaatc
     6481 catataattt gctatcagga tttcccgctt taataccgtc atagagacca gcgataacat
     6541 tatgtcctcc gggtgcttgc ccacctgaaa gaataacacc aactcgaatg agttctttta
     6601 ccgcggggtt ttttcccttc acaaaatggg caattggttt gccataagtg tttttaaata
     6661 attcttttaa agccgcttcg tcgctaattg cctttgttgg tttaccgaat gctacctgaa
     6721 tagtgttaag gcgcaatttt agaactgcgg gtagttttgg tttgtacgca taccgtgcat
     6781 tttgcaatgc agaaatctta gccatagaaa attctcctat ttcttggtaa atattttttt
     6841 catttataga aaattctttt atataatata ggaaaagaaa tagagtttca acagggtaga
     6901 agctctctaa gtctactcag tttcaataaa aacttcttga agcgattctg tttcaatttt
     6961 attttcaatc tcgtcttcta gcttgtaaaa aaaatctatc cccgttattt tttctacttc
     7021 gcgtatggtg caaaggtatt ctgaaaaatg tttttcaccc tttgcattgg gaattatata
     7081 ggctgcggca atagctgcaa aaaacaagtc atctgtttct ctatttcttt ttacgattgg
     7141 tgataaaaat attttataat aggcatcagg tacagcaacc ttgtttatgc ctattgtctt
     7201 gtatgctttg ttttcaagaa gaggtcccgt aacaacatag acgataccgt aacgctcagc
     7261 ccattttctc ccttctgctt caagatttgc ccaaatacct ctattcatgg ctggaacttg
     7321 cggagacata ttgctcatgt aaaaagtttc gctcatagcc agagaatcaa aagcaaaatc
     7381 tgctgcaggt gcaatatgcc ctctgtcata accgctgccg cgatagtctt caaggcttgc
     7441 gcttcctgta cttatttttg gatctacccg aaaattgtct ttacgacttg tgtttttttc
     7501 aagtttttct gtagtgatag tataggctgc ccattcgcat tgttcaaaag attctctata
     7561 acataattgg tagtattcaa aaacacgtag ttcatggtct tgtttttttt caaaaaattc
     7621 tatcgtgcta tcttcatcct ctgttttttc ttcagctatt gcggggcaaa gtggaatttc
     7681 taaaaaagaa atttcgaaaa agggatgctg tttttcaaaa gatttttctt cagcatatag
     7741 gggaaagtaa aaagaaaaag ctaaaaaaag aaaaacaccg agtccttgcc ttagccattt
     7801 ggttattttt ttatctaaca tcactttatc cttgttttat ttttacccat ttcgcatcga
     7861 gctcttccca atgaaaggca tattcccatt ttctatccca ggaaaactct agaagggcaa
     7921 gtttaaaaac gcgaatttct gagggaagat ttttttgcaa atcttcgacc gaatgaattt
     7981 cttttttacg ttccgttaca tcgttaaaaa agttgctaga aaaaactgtc tggctgtctt
     8041 catttttttt tacaaaaaca agcggcatag aaatggtgtt tggaagaggg gggaaatctg
     8101 ctatttgaat ctcagtccta tgttttttta tttcaaagct tatgttaaag gcgaggctga
     8161 gccattgtcc atgcctttct atttctctta gctgtacggg atttttcttt ttttgaaaat
     8221 cgtttttcat agattctaat atttcttttt gttcttttat agtttgcttt gtattgaata
     8281 aaacaagggg acaaaataag ggatgcatgg gatagacttt ttcatttaca agtctcgcct
     8341 gtattttttt taaaactgca atttcattgc cgtggggtat gagtgcaagc aaatttattt
     8401 tattatcttc catagtgcct tcctttttac agctcctgta attatagact ttttattctt
     8461 gaaagtgtag tatatttaca tgaagaaagt ctttgttttt ataattactg tacttttaag
     8521 tataatgagt cttgcttctt gcaattctgt ttcaaagtct tctattgcct tagagctaga
     8581 agacctttcc ttttctttgt ttgcacacaa tgcaaatgca aaaaaagcat tcatcccatc
     8641 tcagggaaaa agtccctata tgtttttttc atttgatgat tccttccatc tctttgttga
     8701 cgagtataag gatacaagtc ttgcactctg cgttgatata gattttgcta aagataggac
     8761 agacgtaaaa aacaggggaa aggaaaacaa aatagccttt ggctttttga caaaagatga
     8821 ttttacaagc gatggtctgc taaaaacagg cacactcgtc agccgttcta tggtaactgg
     8881 ttttattgac agtgcagatt ttcaagaggg aatgtcgctt tcttttagtc tccctcagga
     8941 tacaaagata aagggttttt tcttgtacgc aacagaagac ctgtatctta aaaaagcgcg
     9001 gatacaaaaa ccttctctcg gttggtcctt tgctgaggag cgagcttttt ttgccttttc
     9061 tgcaatgggc ggggagattc cgtcaagttt agcacaggcg caagatgagg gactttctct
     9121 tgccttgagt tcttccctag aactcgatac aaaggagtcc tatctttctc ttgctttttc
     9181 cccacaagaa caattgagtc atcctgaacg ttatccctcg gttgttctcg acttggacaa
     9241 agaaagcttt actatacaaa gatctccctt gcaaacaaag gcacatatat acgcctcgca
     9301 aatgctttct ccaacagcgc aggttttttg tagtcaaaat aaagaaatgc ttagcgaatt
     9361 actttttatg tcaaatgaag gtataagaaa aaaaacgtct acggagaaaa gccttgaacc
     9421 gcttcacact gatccaggtt tgataattat ttgggataag gccagctggc ggcaagaaag
     9481 ctatgagctt ttttcttggg agctttttcc ctctgtcctc attatggatt ttaaaaatta
     9541 tgcaatccaa gatgactact taaaacggct tgcttttttt gcagaaaaaa agggctatac
     9601 tggtcgcttg agttacgatg atgaaataaa atccatgcac ggtttcaatg cgcatgacta
     9661 tagcgcagaa acccttgcaa acttttttgc tttagctgag aaagagaatt ttccattaaa
     9721 tcaaagtgag ttgcatctac gttctattct tttagaaaac ggggtgatac gatttgaaaa
     9781 tggaaaactg gtagaaggtg cgggtgcact tttaagtatt tcacaagaat cgactcttgc
     9841 gcttcgctac acactcatgg gacacgaagc ctatcatgga atttatttta cagaaaaact
     9901 ttttagagac cataccctcg aagtatttaa tgcaagtgat ccaaactcgc gcgattttct
     9961 catgggatat tttacaactt cagaaacttt aaactatgac atagaaaacg attacttgtt
    10021 gaaaaatgag tttatggcct atcttttaca acagtcaatt gcaaacaccg gccagtattt
    10081 tgtgaatatt gcttctcgac cctctgttat aaaaaatttg cccgagctga gtctttacgt
    10141 gagaaatagc agagggagcg gttttactga agcctgccgc gagcttgatg cctttatcta
    10201 tagatcttat ggcttggcag ctgggcgagt tcgtctagtt ggcagaacac ggagctcttt
    10261 ttagaagctc atagaaagat ttgctcttag cccttgtcat caaggccact cactgttctt
    10321 actacaacag aagtgttaaa ttttttttca gctgcaacag acgagggtgg tccatgccca
    10381 ggcataatta cagtgtcatc gtgctgactc aatattttat tgtgaacagc tgcaagcaac
    10441 attctttcgg cataggtatt tgtagttgaa ccgagggtgc ttgctgtcag tgcatcccct
    10501 gtgaataaaa cctttcctat tttgaaaacc atggagtcag gagtgtgccc tggaacagac
    10561 atataaccga cttgcaatcc tccaatgcgt aaaataccat catcttttag aatttgcgtt
    10621 tctcgatctg caacttcata atccgccgca taaattttgg gagtgtagat ttttcgtagt
    10681 gttgccaaac cctttaagtg gctttcgtgg ttatgcgtta ttaaaactgc ttcaagattt
    10741 aattgttctc gctctatctg cagtagaatt tcttttgtaa cggttccagg gtctatgatg
    10801 atagcagctt tttcttcagg gtttgttaca acatagcaat ttgaaaaacc ttcgagtgac
    10861 aaattaaaat aaactttcat aatctatttc ctttgaaaaa agcgcttccc ttgtatttga
    10921 aagtttaaaa gagacatttt cttgactcat ttcaaaaaac attgtttttt taatattgca
    10981 gtttctaaaa gaagtgtttt gcaatctggc acggataaaa cgtgagttat acaagtctga
    11041 atcgtcaaaa gaagactgta aaatatggct gccacaaaaa ttgttgtgga caaggtcaga
    11101 gtttgtaaac aaaatatgtg aaaaagttgc gccagcaaat gaggtaaatt gcaagtttga
    11161 cccaagtaga ttgcagtcgg aaaaaataga aaaatcgaac atagacatgc ggctacgtaa
    11221 gttttcagaa tggatattgc gaaaggtaca accgtaaaag ttgcaaccgt aaaatcgttt
    11281 gtttgtaaaa tctaagtttt gaaaagagat tcctgaagcg ttgagaccca ctatcttgtc
    11341 gttagatttt atatactcat atatatactc ttcgatttgt gaggctatag gcgaatgttc
    11401 ataacaatac tcatgcgtgc taagcgtttt tgtatctttt gagtagtttg ataccgctgt
    11461 tttttggcaa gatggatgct gacaaatcac ataactaaac atttttacat agtaaggcat
    11521 tgctaaaaat aagtcaaatg tattatcatg taaaaatgca gaagaataag acttgcactg
    11581 attttttatg tatcgattca ggctcaggtg ggcttcccta tcttttatat cttttagaaa
    11641 gatttcctgg ttacagctcc gtctatgttg cagataccga aaactttcct tatggaaaaa
    11701 aatcgaagga aaaaatcgtt gaggcaatta ccgccttagt cgaaaggctt ctcgaacact
    11761 ttgaaccgaa gattataatc atcgcttgca atactatctc tgtaactgcg ctcgatattc
    11821 tgcgctcccg tttttcgatt ccctttgttg gtaccgttcc tgctataaaa caagcggtac
    11881 agttaagtaa aaataagatt ataggacttt tggcaacaga aaacacagtt gcctccccct
    11941 atactgatgc tcttttttta gaatatgctt cagaatttag gcttattaaa agaggcgatg
    12001 caaaacttgt ccgttttgta gaaaaatccc ttcacaaggc agatgaaaaa aaacgaaaaa
    12061 aagcactgaa acccgctctc gcattttttg aaaaaaacaa tattgacgtg ctcatccttg
    12121 gctgcacgca ttttattcac cttataaacg aaataaaaaa ctccgcccct tcgcattggc
    12181 agattcttga ctcaagagca ggcgttatca atcgtgctat acatatagca aacgaaaagg
    12241 ggatagttcc tcgtttagaa agcaaagcct tgttttatgc aacaaagcag tacagcgctt
    12301 ctgaagaaat actacttcgt agctttttag aacagaatgc tatttcctgg caggggctta
    12361 tttgacattt gaatatattt tcttgtgttt tttactatta tgctatagaa tagtcttata
    12421 gtaaccaatc gtataaaaaa ttattttgga ggaaaagggt atggctagaa aagtttcgtt
    12481 aggagtgttt tttttacaaa tagcacttgc attatttctt ttattttcgg ggctcaatgc
    12541 aacatctgca gtggtgaagt cggattcttt tggattcagt gcaacaatta gcagaaactt
    12601 tggggataat gaggttgtta ccattgtcga tcaattatta cctaaaaata aatcgcttgc
    12661 aactggcatt attattattt tagcaattgt gcaaattgct tgcggtgcta ttttgctttt
    12721 aaactttttt attgagacaa agcaaattac cgatattttg ctcataatta tgctcgttgt
    12781 atgggcgctt attattatat ttttagatat aataggtacg ggtggtttaa tcaatggtgc
    12841 ttttaaaaac tacaagacct ttgttgcatt ttgtaagcaa ctttcacaac accttttggt
    12901 gattggcgct atattgcttg cgttcaaaaa tgaataaaaa aactgaaact aaaatgcgtc
    12961 aataaaaaaa gccttgcttg cgggcaaggc tttttttgta tttagttaat cgaagcgatc
    13021 catagacgaa tcgaacgtcc gacctagtgc ttaggaggca ctcgctctat cctactgagc
    13081 taatggatca tgtgttttta tagtacataa aaaatctgtc ttttgcaaga caatataaat
    13141 ataaagagga aggaatatgg caagttttgt attattattg ctgtttttat ttgtgatgat
    13201 agcggttggt atttggagta tcagaaaaac aacgacgctc aatgattttt ttctaggagg
    13261 gagaaatctt ggtccttggg tttctgcctt tgcctatgga actacttatt tttcagcggt
    13321 tatctttatc ggttttgctg ggaagcaagg ttggagtttt ggactcaatg cgctttggat
    13381 aggtataggc aatgctattc ttggagcttt tcttgcttgg attttgtttg cacgccggac
    13441 gaggaggatg acacaaaatc tgaacacaat gaccatgcct gaatttcttg aagcacgcta
    13501 tgaaagcgac catatgaaaa tgtttgctgc cttgcttatt tttgtctttt tgcttcccta
    13561 ttctgcctct gtatttaaag gtttagggca tctttttgaa atgacctttc atatatcctt
    13621 tgattttgcc ctcattattc tcattgctat aacaggaatt taccttgttc tcggtggtta
    13681 ttttgcggta actattactg attttattca aggctttatt atgctcaccg gtgcgattgg
    13741 tatggttatc gtactcgtta aaaatgctgg cggtcttttg cctgcgattc aaagtattca
    13801 agaaaattat gtgcagcatg ttcctgtagg ggagagaccc tcttggctta cgattgcttc
    13861 tcttattttt atgacaagct ttgggacttg gggtttacca caaatggttc aaaaatttta
    13921 tgcgctaaaa aacgaaggga ttataaaaaa agctgcacta attacgactg tttttgccct
    13981 cgttattggt gtttcagcct acatgacggg agctctttcc catgtatttt ttagcccaga
    14041 aagcctccct cgtcttgagt caggtgcaat aaattttgac atgattgttc ccattctact
    14101 gtcaacacat ttgcctgaac ttttgttggc aatcattatg ctcttggttc tctctgcctc
    14161 tatgtcaacg ctcgcttcta ttattcttgt ttcttcctct gccattgtaa ttgacttgta
    14221 caaggggtat ttgcacaagg aagtgagcga ctcccattct gttttactca tgcgagtcct
    14281 ttctgcagtt tttattttgt tttcgtatat tatttccaaa atgcaattag ggtttattgt
    14341 aacgcttatg tcgctttctt ggggtgtttt atcgggtgct tttatggctc cctatatttt
    14401 gggtattttt tcaaaaaaaa ttacaaaggc aggaagctat gcaggtttgt attctggggc
    14461 aggtacagct attattctca accttgttct cgctgcgcaa aactctccgc ttgcttcatc
    14521 tatagcgatg attgttccct ttcttgtcgt gccgattgta agcgcattta cacaaaagcc
    14581 ttctcaagag cttcttgaaa aagcgtttac aggtatttaa ttttctagcg agttagattt
    14641 tttagttgtt ttttggtgca aggttttttc aaattcttca acaggcattg ggcgatagaa
    14701 ataatagcct tgcgcccgat tacaacggat agactgtaaa aaatcaactt ggtcctttgt
    14761 ttctacccct tcaacaatgg tcggtaaatt tatagcgtga gacatattga ctatggattc
    14821 aagaatttgc tctcctcggc caagaatatc gcttcccgat aaaaaatgaa ggtcaatttt
    14881 taatgcatcg acgggtattt ctttgagcat attgagagaa gaataacctg agccaaaatc
    14941 gtccatcaag accgtaaagc cattttcgtg taattcgtga atgagatcaa ctaaaagata
    15001 ggcgttattt gtataggcgg attctgttat ttcaagttcc aataagcgcg gtggtatgcc
    15061 atatttttct gcaagatttt tgattatact tgcgagttgg ggattaaaaa ggtttacccg
    15121 agaaatattt accgagatag gtaaaacagg gcttccttca tctatccatt tttttagcag
    15181 tttgcaagct tcttcccaaa taaattgatc gagagccata acaaaaccat ttttttcaaa
    15241 aataggtata aatttccctg gaggaataat gccttttttg gggtgtgccc aacgaacgag
    15301 tgcttctgcc cctacaattt ttccatcttt taagtcgcat tttggctgta gatacatttt
    15361 aaattgccct ttttctaagg catcttccat ttcattcaca atttcttgtt cttcaataag
    15421 gatttggcgt aaggcttcat cgtaataggc aaagcgggta atatagttcc cctttatagt
    15481 ttgtagagca agattcgctc tatctatcat gctgtcgatt ggaacatcag tatctgtaat
    15541 tatataaaga ccaaaacaga gaataatttc taaattgaga ttgtagagtt tcatgctatc
    15601 ggtataggtt tgcacaagtt tttctacatt ttcttttgaa tgtggaaagc atgcgacaaa
    15661 gatgtctgat gcgattcgtc cataggtacc aagatttgaa ccatattttt ttaatatact
    15721 tgcaatatgc tttaaaagcg catctccttc ttggtgcccg agaaactcat ttatgacttt
    15781 gaatttttct atgtcgaagc gaacgagcac ataggtcgtg tctgggtttt tttgaagcat
    15841 ttttttagtt tcttgaacaa acattgtttt attataaatg ccggtcaatt ggtcatattc
    15901 agcaagtttt tgatactcat tttttgattg cgtaataagg tttaataaaa cccgttcctt
    15961 ttggtgttgt tcgagcatca tcgtgtgttg gatacgtagt ttatcaactt cttttcgtgt
    16021 taaaatattt tgcacacgca gccgcgctat atggggcttt ataggtttta caataaagtc
    16081 agttgctccc agttcgagcg ataataactc gccatcaatt tccccagaag cgcttacaac
    16141 aacaatgggg atatttgaaa gttcttcgtg agcgcgcact tctttgagca cagaaaagcc
    16201 atctttttca ggcataacta agtcaagtaa aataatatcg atttcagtct ttttttcatg
    16261 aagcagtttc atagcttcta tgccattttc tgcttgcaga atatgaaact cgtctttaaa
    16321 tatattttgg agaatatcgc gattcatttc aaaatcgtca acaataagca ttgttttttg
    16381 ttgtaataga atttctgtga tcattgaatt ataaatcctc cagaattatg taagctaggg
    16441 aaaagatcta acatcactat tagtatacta caaaaaataa aaaaaaaata ggataaaagc
    16501 ttgaatattg tacttttctc ctatttcgaa tccatctttt aaaataaaaa aggaaccctc
    16561 tcggattcct ttttttgagc tacgatggat tcgccttcca ctcgaaaact tcctgttttc
    16621 tcgttccagg ccgccttcac tccctgccgc gcacgtcctg tgcgcttttc ctcgctatgc
    16681 gctcggcggg agttccgttt cgaatccatc tcctaactaa acaaaaaaag ctgcccaaaa
    16741 ggacagcttt tttttttgag ctacgatgga ttcgaaccat cgacccacgc cttaaaaggg
    16801 cgttgctcta cctactgagc tagtagccca acatttgttg tttgaatgtt tgactacttt
    16861 actgtattcc ttaaataatg tcaatacgat tattgagaat tatttaaaga attcgaggga
    16921 aattgcaagg ttgagctgtg gtcgagtgcc tggttcaagt ccaagttcgc gaccaaaaat
    16981 cgctgcatca agattgaatg ctccgaatcg agcaccaata ccagctgatg gatacatttc
    17041 atgtaagcct aagcggagac tgattgtttt gaataaaacc gcttctgttc caagtgatac
    17101 atcaagaata gggtttcgtt ttttggggtc atttaccatc atgccgataa catcgtgcaa
    17161 gtcgagcatc acattgagcg atgaaataac acctagggta aaaacattta cactaggtac
    17221 cgaaacactt gcaccgaggt taaacttcca gtcgaagtta aatgcctggt aacttgcatc
    17281 aggttcgagt tctcctgaag ctagattagg agatttactt gtaaaatagc cgcttacagc
    17341 atcatcccaa acgagtccta cggtgataaa atcgagaaga ctgagcgttg ctccaagatc
    17401 taaaccgaag ccaaagcttg tgtagagggg gagaggcatt tcttcagaat tgccactcat
    17461 aaggtttgta acagaggtta agatttttcc ttctactcta tttactaagt caactgcacc
    17521 tccaacggaa agttttccaa tgccgagtgg aatagggagg gcatagccaa agcgtagtaa
    17581 gctttcgttt cctacaagac cttcaagata gctaatgctt ggagcatttg caataacata
    17641 ggtttgatta aaaaagcccc aaccaaagtt attttttaca gagccaaatt gaatgggccc
    17701 tgaaatcact acatctagat ctaagccctt gttttcgctt aggatttttg caagcgagtt
    17761 gatagattca ctgttttcca tgtcgtttgc aatagttatg atatctgaaa gatatttaat
    17821 aggtccaccg agaccaattg ttaagcctgg atacaaactt tttttaccca taagaccgat
    17881 atttccaggg tttgttaaaa agctatagta ccagcttgtg tcgcttatat gcttaccacc
    17941 aagggcgtct atccttgcat gtcgaatttc tggcatagca agctcgtagg taaattcttg
    18001 agctataagt gcaaagctcg ttataaccaa aaggattgcg agtatattat attttttcat
    18061 tctttacctt caaataatct tctaagctca gaccaaagag agtgagttct tcatcgcttc
    18121 tctttccacc ttcgtcaaaa agatctaata cagctttaac agcttcgata tctgccttac
    18181 tttcgggaga aatgtcaact ccatcattat catcattaaa tagatctgct aaatcgtcaa
    18241 agttcaaatt ttcaccttct cgtttcatca cttgtgcaac gagtgcaagg gatgcggaaa
    18301 caaggctttc tggacttgca gattctaggg catcttcgtc atttaaaata gcttgtaaaa
    18361 cggtagtgtc aaaactattg atacctccca gaatcgtgtt aaaaagttct tcaggctctg
    18421 cattatctgg atcttgtgct atatcggcca attcagtcac aaccgaggtg gggatagaaa
    18481 ccgctaaggc aaggtccatg atgctttctt gctcagatac cgagagcgct tgtatgtccg
    18541 cctctgattt accgctgagt gcattaagca cagcaacacc tgagctaggg tcctggaatt
    18601 gtggatcttt tgcaagcttc gctaaatctc cagcactctc ttttttaagt gcagtactta
    18661 agtcacgctg cattcctgag ccccacgatg tgacaaaaaa atcacaagat ataaagctaa
    18721 ataataaaaa tacgctaaat acgcctataa ttattttttt catatgtacc tcctatggtt
    18781 tttggtgtaa atctctaaaa taatacatag taatctatgt tatctcttgc agtcggctta
    18841 attgtacgtc gctttccgaa aaaacacaag ctctcgcatc gattttattt gagctttcat
    18901 agcgttctac taggattcta atctcttgcc ctgcaagcat ttgcgcttct tttgtgttca
    18961 ggttactgag ttccacatgt aaaaagttgt cgcaaacagc acgagcagga atactccttc
    19021 ttttttccac tattgctagt cgttttttac cgacttggct taaaacaaag gctttttttt
    19081 gttcctttgc aagctctgtc attagtataa cacgcttttg aagaatttct gcacttattg
    19141 tattttttaa taaaaaagct tcggttccag ggcgcgcaga aaaggggaag acatgtatcc
    19201 atgcaaaatt gagttctttg caaagcgaga gtgtttccaa aaagtcttct tcagtctcgc
    19261 caggaaaacc tacaataata tctgtcgcta taaaaacatc ttcctttgcc ttacgaatca
    19321 aatgaacagc ttcgctcacc ttttttgcat tgtaggggcg tttcattgct ttgagaatct
    19381 tatcgctacc ggattgtaca gaaagatgaa aatgcggcct gatgcgttca gattgtatga
    19441 gttcgcaaaa ggcttcatcg acagattgcg gatagaggct tgaaatacgg aaggcaatgc
    19501 cctcggtatt ttttatgaga tagtctaaaa gcgctgtgaa tcccatcttt gtttttgggc
    19561 aaaaatactg gctgaggttt atgccagtga gcaccacttc tttatgccct ttttctatca
    19621 attctcggat acgatcgcaa attgtttcgg catcgaggga aatagcctta ccacgagcga
    19681 gtcgcacacg gcaataacta caagcgctat tgcaaccatc ttgaattttg agagaagctc
    19741 gtgaatgcga aaaaaagaca tcggtagata atcgaaaggc aaggttttct tcctcccttg
    19801 tttttgtatc tatccattct ttgaggcttt ctagatgaaa ttttttttgt aaggaagaag
    19861 cggggaaggc ttcggcaagg tctgttagat agcctttttt tgaaccaggg aggataatga
    19921 cacgattatc tatttcttcg aggtaggctc cgtcaagttc cgcatagcaa ccagttacga
    19981 taattggtgc atcagggcag aggtccaaga gcatatttat aaggcgtcgc gctttttgct
    20041 cagcttttcc tgttactgtg cacgtgttaa taatagcaag ccctatatct ttttgtgtgc
    20101 cgcttgcccg tgtgagattt ttgcttgaga ttatatatcc ttggtcataa aaagcctttg
    20161 cagcactttc gctttctatt tgattgagct tgcaaccgag tgtttcaaaa tgtattgtca
    20221 ttatctttcc ttagaaataa aaagcatcga aaaaaggtat tgaaaacctt tttagcgaat
    20281 gagttttttt actctctctc tgccattgat agcatcttct aaaaagggac ttaaggaaag
    20341 cgctttatcg taggcaagaa gggcagagcc atagcttctt gcaagctccc tggtatatcc
    20401 aagccttatc caccaaaaat ctttcagcgg ctctattttt acggcttgac tgagtgctat
    20461 atccgcatgt tgatactttg cttgtcgaat aaaaatttca cccataaaat aataggcaag
    20521 tcctgctctc gatgcagaca ggctaacgag tgaaagatat tcttgaaata agcttaaggc
    20581 ttcttggttt ttcccgaggt agaattttgc ttcggcaagc gcttctataa ttctctgatc
    20641 gtattgggct atttttcttg cgtgatttgc ccaaaattct gcttcattgt attgcttgtt
    20701 tgcgatgagg ctccagcaga ggactgcata ggaatccatg tttttatcat ttacatgaat
    20761 ttcatcctcg caaatttcta tcgctcgtat ataatttcct gcattatatt cttttaaggc
    20821 gtcgggtttt gtttgtgtta ttacaaaaga gacgcttaag agcaataata aaacaaagcc
    20881 aacgtttttt ttcattatga accttatttt tgatttgtca taacgcaacg accagtaaaa
    20941 gtgcttcctg gttctaagac aacttgctcg gcaaaaatat cgccggtaac aacaccagta
    21001 gatgcaacaa agacaagtcg ctttgctttt atatttcctt caaccttacc gcgcacgaga
    21061 acacgctcgg caactatgtc ggcaaaggtg gaagcgtcgc tatctataac gagatcgctc
    21121 gtcgcttcga taagtccggt aactgtcccg cgaatcataa acggtttgga gaatcgtatc
    21181 gttcctgcaa acgaaatatc gcttgccaat attgtatcaa aatcttcttc ttcgagttcc
    21241 aataattctg aatctttccc tttaagcata actacactgt atagctattt ttttaaatcg
    21301 tcaataggaa gaataattgc tgggtacttg tgatttttaa tagataagag tattctgtat
    21361 gtgtaaaaaa atatagatta aggatgaaga agaatggatt ttgttcaaaa gatgaaaaca
    21421 caggcacaaa tgcttcaaaa tcgacttgta ttacctgaag gtagtgaaga gcgcgttatt
    21481 tttgcagcag aaaaaatcat gcgagaaaaa ctagcgaaag aggttttttt aattggcgag
    21541 gaaaagaaaa ttaaagaatt tgctaaggaa aaaagcattt ctctcgatgg catcactatc
    21601 cttgacccaa ataaatcaaa gttttttgat gatttttgta cgacgtatgt agaactacgt
    21661 aagcataagg gaatgactct tgaacaagca agaatagata tgaaagaaaa taatcgcttt
    21721 gctgctatga tggtgcgaaa gggcaaggct gattccatgg ttgctggagc tatttcgaca
    21781 acagcagatg tacttcgcgc aggtttaacg attataggca cagcagaagg aacaaaaact
    21841 gcctcttctt gttttgtgat gcagatgaag gactcttttt ggggtgctga aggacttatg
    21901 attttttcag attgtgctgt tatcccaaaa ccaacagctc agcaactttc agacattgca
    21961 atttctgccg gccagtcatg taaaagcctt attggctcag atcccgtcgt tgcgatgctt
    22021 tctttttcta ctaagggctc aggaggcaag gacgaagatg ttttgcgagt ccaagaagcg
    22081 gtaaaacttg cgaaagaaaa agatccaagc cttttaatcg acggcgaatt acaagcagat
    22141 gcagccctcg taccttcgat tacacagaaa aaagcccccg acagtcccat tacgggaaag
    22201 gtaaataccc ttgttttccc cgatttgggc tctggaaaca taagctataa actggtacaa
    22261 cgcctcgctg gagctgaagc cttcggtcct tttttacaag gctttgcaca acccataagc
    22321 gatctttcac gaggttgttc tgttgaagat attattgcgg taagcgcagt aaccttagtt
    22381 caggcaggaa aaaaagaagc ttagttagta tggaagaaag aacaaaggtg ctagagtcgc
    22441 tttttaaaca agcagtacgc gaagggcaaa aaagaaacta taaaaaggcg acagcgcttt
    22501 tagaatcgat tcttctacag tatccacaaa aacagtcgca gggctatgaa aaagacttta
    22561 agtgcaaggt tctcttatat ctttctcgtt cctatcacgc catgggtgat tatgccatgg
    22621 cgatttccaa cctcaattcc tatgtgaatc ttgcaaaaaa agaccctgac ggctggtttt
    22681 ttctagggcg ttcatacctc gccctttcgt cctattctgt tgcaatacag tgcctaaaaa
    22741 aaagcctaga atttcgtcca aaatcggaca caacgcttgc gctcttgggt acagcctatt
    22801 ttaaaagcgg aaaaacgaat ctcgctgtag aaagctttaa agaagcgctc tacatcaatc
    22861 ccgacgatgc acaaataaac caagcctatc aaaacagctt atttgttgaa gcgataaaaa
    22921 tatacaaaag gggcgatata gaacttgcga agcggatgtt tgccttttgt atcaataatg
    22981 gcatggattc tgtgctccct cgcttgtatt atgcacattg cttacgagaa cagaaacatt
    23041 tagaagcggc gctcgatgcc tacaaagaag ccatcgctct tgcaccagat gatccctcgc
    23101 ttcgttggta tgaactttcg attctgagca tgatgggaaa aacagaggaa gctcggcgat
    23161 tggttgagag cctctctgtc gaatttgacg acttcgattt ttccctagaa aagggaaagg
    23221 atcaaaaagc acaatcactt gaaattatac aaaaagcact tgcagaagaa aactggaaag
    23281 aggttatcga agcaggacgc ctgtatatac aaacctttgg aagcgatgcg attatccata
    23341 gttttatggg ggaggcatct cgaaatcttt cacggctaga agaggcagaa aatcattttc
    23401 aaaaggcaaa gagactcgac ccgcagtatc gcgcctatcg ctatggttta ttgattacct
    23461 atctttctat ggaaaactgg gaagcattag atgcagagct tgcaaaaaat aatagccaaa
    23521 gccccttaga cgatgaaact attgcttatt attcggttat ttgcgacgca aaacttaaaa
    23581 aagagccaaa gacggttcta gaggcagtgc aaaagatttt tcgaaaaaat ccagccgata
    23641 cctatatgat gggagttttg gctgaacaat acatacgcgt cggattacct gaattatcac
    23701 agccctggtt gcaaaaactt cttgacttgg gcattgcaaa tgaatcacag tatctgcttt
    23761 taatcgaatg cgccgaacaa ctaaaaaata aaaaagactt tgaaagcgcc tgtcttcgct
    23821 atctagaagg ctggcaggca aatatcgata tacgaaaaaa actcattcgt ttttattcgc
    23881 gccaagaaaa atggaaacaa gctgcaaatc atatcgaaac cttactcgcg tattcaaaac
    23941 atcaggaaac actgacaaaa aacctcgctg tttttcgctt taaggcaaga cagtttcacc
    24001 acgcagctat tttataccgt gctcttttac aagaaaagcc agatgataga gaggctatgc
    24061 agcgttatgt tttttgttta tacaagctcg atatgattcc acaagcaata cgttttttgc
    24121 aactttggca taaaagctat tccgtagatg ttgacggcat tcttattgaa gctattttac
    24181 acttgcacaa tgaagagagc gaaaaagcga ttcagctatt gcgtagttct atggaaaagt
    24241 ttaaaaacga caagaggatt cccaaaaaac tcgcttctat ttatcagaat cagggaaata
    24301 tcgatatggc ccgtcaattt gatccaagca ttagctatac taaaatacaa taaaaaaatg
    24361 atttgcaatt ttttacttct cccattatac tgatatcatc gtagtctttt aaagataata
    24421 cgatgtctta caattttggg aaaaattgct gtgtatagca ttataatcgc catttatgac
    24481 gataggagga ataatgggtt tcaaagccat ggatttatat gacgcatata ataaaaatat
    24541 gctgccaaaa gaagaaggat atgttgtttc agcgtatttt gtaaacggtt cgccatattc
    24601 tatttatgag attatctcat atgctggtgt taaaaatttt tatgcatctg gcgactctat
    24661 taccttccag tcggatggta aaaaaattta tattttagta gaaccgacca gttataccct
    24721 aaaagcgcaa gaaccctatg tacggccgca gtcctatcag attccaatgc gatttaatga
    24781 attggaaata tatacctgtc gaaatcaata taagcttatg tacagcaagg aagcacaaat
    24841 ggttatgact tcctttacag ttatgaagcc tacgggttca aacttttctt ttattatatt
    24901 tccgaaagac gatatagaac aaacaataac actgcttttt caaaaagctt ttaatggtca
    24961 gtcaaatgtg ccgctttcag atgcaaaaaa ggttgcaaca agtctcgctc aaaaaataat
    25021 gaaggagtta agctggccaa ataaagaaaa aaatgcagca aagaaaaaag ctacagaaaa
    25081 agcaaaaccc ggtccaaagc ccaaggcagc cgcaaagcct gcagaaaagg caaaacccgg
    25141 cccaaagccc aaggcagccg caaagccagc tgaaaaggca aaacccggtc ctaaaccaaa
    25201 ggtagcagca aagcctgcag aaaaggcaaa acccggacca aagccaaagg cagcagcaaa
    25261 gccagctgaa aaagcaaaac ccggtccaaa gcccaaggca gccgcaaagc cagctgaaaa
    25321 ggcaaaaccc ggcccaaagc ccaaggcagc agcaaaacct gcagaaaagg caaaacccgg
    25381 cccaaagccc aaggcagcaa caaaagctgc agaaaaggct aaacccggac caaagcccaa
    25441 ggcaaccgca aaaacaatag taaagaaaaa ataaaaaaaa gccctgtatt tccggggctt
    25501 tttttgtgtt taaaggaaaa ctatatttgc aacaattgtt ttaagtcgcg tatttcttgt
    25561 gccagtttcc ccctgtcgag gttttgaaaa cgctttggaa ccatttgttt gcttgtacat
    25621 tctaagaccg caacgagatt ttgtaaggca atttcatgtg gatagctcgg tggaataaaa
    25681 tcgcgtatgg tatgttccaa atctgccttt gttacaataa tacgcttgtc catcgccgca
    25741 tgcatcttgc ttctcacgag aatagcttct atgtcagcac cagagatttc aaagttgatt
    25801 tttttgaaaa catcatggaa ggaatcttgt tcaaccttta tactaagctt ttttttcaag
    25861 gcttcaaaaa gagcgatttt ttcttctaca gaatcaggat aaaacaaagc gagatgttct
    25921 tctgcccgcc cttggcgttt aaggtcaata ggaatgagat caggtcgact cgtgatcaaa
    25981 aaccataata ttttcccacg ataggctgtg tttcccataa acgaagcgat ttgtccaaaa
    26041 atccgactat ttgaaccatc tccgcgatta tcgtctcggc gaccgagtag ggtatccgct
    26101 tcgtcgatca taacagcaac gggagaaagc gctttgagaa tattaaagac gcgctcgagg
    26161 tttgtttcaa caataccatt tgcttgtgag gaaaaattgc aaagacgtac catggggatg
    26221 ccaatttccc cggcaaaggc ttgtacaaga aagcttttgc ccgtgcctat gggaccggat
    26281 atgagatagc ccatgggaag tacatcgagc tggttttgtt ttattgctcg cgctgcattt
    26341 tttaaacgcc caatgacaaa gtcatgtccg cttacagtcg ataagtcgtg gtttgcatcg
    26401 ataaactcga taagtcccgc tccctctttt tcaagaatct cccgtttttt ttgcctcaga
    26461 tagcttaagt cgatgggaac atcctcttga aaggattctg ttgcgatttg gtgcaaatgc
    26521 caaagattca agccggctga caaggcactc attttttcac ttgtcatacc gcgctcgaga
    26581 aggagtaaat cttgacttaa aagatagtcc aaaaaatgct ttcgtaaaaa agcatcgggg
    26641 aggggaatgt gtacctttac taccgaggga gaagatgaga tacggaggtt tatatcgctg
    26701 acattttcgg tgagcacaat gatggaaaca tcaccctgcg taaaaaaagg ttcatgcgac
    26761 catcggttga gtgttacaaa acaaaagcgg tcagtatcgt ccaatagacc tatgttttcg
    26821 tagggaataa tagtttccgc ataatcgatg agaaaaacaa tacgcttatt gcgtggtata
    26881 ttgattaaaa aatatttttc gaggtattca aaagccagac gtggatcttt tgataaaagt
    26941 tcagtatcgc tgagttttgg aaaaaaatgc ttcattgttg caagatattc tttttccatt
    27001 tccaaggtac aaaaacttac gccgcttgag cgatcatagt aaacgacaat attttcattt
    27061 ccaaacaata cttcggaaat atattcttgt atctttttaa atgaaaactc gccctcattc
    27121 atagaatgcg gtaaaaaatc acggatattg ccgtgtacga gatacatatt gactgttttt
    27181 gaacagtatt tataggaaag ctcttgcgcc cactgtggta atacctttat ataaggcaag
    27241 ttctttttta ttagttgttg ttttccaccc atatgtctat tatgccgtaa agctgccttg
    27301 atagcaagtt ttttatgcgc ccttgtgtaa atattttttt tgtgctagag taaggctgta
    27361 aaaaaattct atagagaggt tgtaaatggc ttacgttata tcaccagatc tttgcaataa
    27421 ttgcggtacc tgtgaaccag aatgtcctac tgaagcaatt agcgagcaaa atgataaaag
    27481 atttatcgac gctgatttat gtatcagttg tggcgcctgt gcagcagttt gccctactga
    27541 agcaattagc gaagaataaa actctttgca caaagtgaaa ccggtggctt ctgttgccgg
    27601 tttttttatc tttttaaatt aaattcgatt atacactcgc tgtataatct gcttaaggaa
    27661 aagaggcttg aaaagagcgc acagacttcc caaaaaaatt cttatttttt tcatctccct
    27721 tctgggtatc agcctttttt gtgtgttttt ttcagccctc attgttactc cactctggtt
    27781 tttggcaacc cgtcttccgc atatttttac ccttattttt ttgagcgttt ttcttttcct
    27841 cattttttat gggatatata aacgtgtaca aaaaaaacaa tagcattctt tgtttctttt
    27901 tttttattct gcttcacact cgctttataa gcgcacaaaa acttcccgaa atctccgatt
    27961 tatcacataa aaatattctt tttaaacaat tttccgaaga agttgaaaaa aattatcgtc
    28021 ttctcgcaca aggaaatacc tccatctctt tgcaattttt ttattatcga gcagaaaaaa
    28081 acgatacgct tttgacaatc gctgcacgaa gccttattcc ctacgaaact ctcgcaagtt
    28141 tgaaccgcct cggcgaagta ggggcaaaca tagaagggaa attgcttatc ttaccgacct
    28201 gtcctggact ttttattctc gaaaagcctt tttcgccatt agaaatactt ctaaaagaaa
    28261 aatacttgca cgagccaaat cttgtatgct atactttggg ggataccttg ttttatttta
    28321 tacaagatgc aaggctcagt ggaacagaaa gagcattttt tttggattca aatcttcgtt
    28381 tacctgtttt tcatgcagtg ctttcttcga attatgggca gagactgagt cctattacgg
    28441 gaaaatcttc ttttcacgaa ggggttgact tagctgctcc agaaggaagc cttgttatgg
    28501 cttgtaaggc tggacaaata gagcaaatag gctattcgca aatctatggt aattttatca
    28561 ttatcaagca cgaggatgaa aaaggtcggc aaatgcgtag tttttatgca catcttaaaa
    28621 caatcgactg taaagaagca agttttgttc tcggtggggc tactataggg agcgtcggct
    28681 ctacggggct ttcaaccggt ccacacctcc attttgaaat ttggttgaat ggaaaaacaa
    28741 tagatccaaa cgaactaata aaaggaatca ggtaatgaaa aaaaaaggtt taattgtact
    28801 ttttttcttc ctcacgcttt tttgttttac aaaagatatg tttcgagtac atgaaacgca
    28861 gcttgtcgaa atgcaagatg agcagcaaag tcttaaagca cgtcttgcta taaacgacat
    28921 gctcgtcatt aaattgccca aaacaatata ttttttacaa gggctttctt tggagataaa
    28981 aattcccaag gctgttgctg actatcgaga tgcagtagct ttttatgtgt ataaaaacat
    29041 gtctccgatg ccgacgccaa aaactattga ttatgcgggc gaccgtgttt ttttgaacac
    29101 ttttccagga cgattgagct ataattttca aataccgctt gccaaaaatc atacgctaaa
    29161 agaaagcccg tattccgctc ttttgccaga agttatcgat gtaaatgaag gctatgtcta
    29221 ctttcgctta caacttgtta tgaagggcac accagtggct gttttagagt ctgaatttga
    29281 tattgaggtg aaacctattt taatcgacaa gggcatgctt aatctcagct taatcccgcc
    29341 gaatgcacat actcgccaac aaattccggg agaagattta aaggctgata aagacattga
    29401 cctcgtcgcg aataaaaaaa actatgctct atttattgat gaaaaacctg ttgatatgat
    29461 agacaatacc atcttactca attcgggagt gcatcatctt gctgttttat ctgattattt
    29521 tagaaatgaa gtacggagtt tttctataca acagggaaaa acgacaaacc ttactatcca
    29581 gcttgaaaca aatgagccaa cgatgcaaat tttagctccc gaaagccttg aagtgtactt
    29641 taacgatgtt ttattgacag atttttcaaa accctatcaa ataactgaag gagagcataa
    29701 actgcgcttt ttgctcgatg gctatgagtt tattaaatcc tttactgcct tgcggggaaa
    29761 aagctatagc ataacgacaa atattgattt ttttattgaa gaaagataaa agcgagattt
    29821 tttctattat ttatcttaac tttttttaag gatctccgat actttaaaag tcaggcagac
    29881 aaatcccctc ccacccattg tctgtctgat tgccgatggg catggccggt ttcctaaccg
    29941 gccttttttt tatttttagc ttagattttt gccagagact gctttttctt acgtggattt
    30001 gaggagattc aatgcgagac cttgacaaaa tcaagcctct ttcataccat aaatctaatg
    30061 gctactatta aaaaacgcac tattttctat tttcttttta ccctattttt tgctcttctt
    30121 tttggactta gcttaggctt actcttggca aataccgcta atacgataca tacggagaat
    30181 ttttacgagc ttgaaattgc ccttcctaca cgattactcg atatcaacgg cgagtttata
    30241 acagaatttg cctcggcaga aaaacgtgaa ctcgtagatt atatagattt accaaagcat
    30301 atgatcgatg cgctcatttc tcgagaagac aaagactttt ttcaacattc aggttttaaa
    30361 gtaaaggcaa tcgttcgagc tgttttggga aagctgcttc gaaaaaattg gggcggggga
    30421 agtacgctca cccagcaaat cgccgggacg gtctatgccg atcgctccga tatgtcttat
    30481 agacgaaaac tacgcgaatt gttttggaca tttcaaatag aaagaagata tgcaaaagaa
    30541 gaaatcctcg agctctacct caataaaatt gtgtttggga atggaaccta tggggtaagc
    30601 gctgcctcta agtattattt tgggcatggt cccgataaaa ttaccccggc tgaggcagct
    30661 attttggtga gccaactttc tgctcccgta tacaataatc cctttgagta tcccgcccgt
    30721 gcaaaggtgc gacaagaaga tgttttaaat aaaatggtca agtatggctt tttaagtcaa
    30781 gaaatagccg cagcttcctt ggaagattat tgggcgcact ttgactatac ccgcacaagc
    30841 gtcggggctt attttttacg tgaggataag gcgccgtggt ttagcgcgca cgttctctcc
    30901 gagcttatca atatgatgta cggaagcaat gttgatgtat acaccagtgg atatacagtc
    30961 aatacaacaa tgaatctaaa acatcagctt gctgcacaaa gaattatgga cgagcgcata
    31021 cgttttgcca acagagaaaa tcaaagaaca gcctctgcaa gtagttcggt tgcagtaaac
    31081 acctatattc cgatgacaga gcttatatcg cttgttttta accttcccag tttgaaagtg
    31141 tcggagcaaa gaagtgagcg acttgcccta tcagcctata aaaatgagat aaatcccata
    31201 ctcgatgtcg cttccttatt gtttggtatg gaagacttaa aaatttctgt agtcaataag
    31261 gcaaatgttg aaaaaagcaa ggatgtgagc aaagattcta tcgaaggttg tttaataacg
    31321 attgaaaatg aaacggggca tatcaccgct ttgattgggg gaagtcaata cagcaccgaa
    31381 aaccaattta accgagcaac gcagggactc ttgcagccgg gaagtacctt taagccattg
    31441 tattattcag ctgctatcga ttcacgaaaa ctgactgcag cgtctaaaat agccgacgtg
    31501 ccaactcttt tttataacga aagcggcgta ccctattatc ctgaaaattt tagaggggaa
    31561 tggatgggaa cagtgcaact ctggtatgcg cttgccaaat caatgaatgt tccttctctt
    31621 aaggttatgg aagctgttgg cttcgatgca gctataaaac gttcgaccgc cttgctcggt
    31681 attccagaaa gtcagtataa aacacgggat ttctctcgcg tctatccttt ggggctggga
    31741 acagcaattg ttaagcccat cgagcttgcc cgtgcctatg cggtttttgg aaatcaggga
    31801 agagaggtta cgcctatatc tattttgtcg gttgaagata aaaaaggcaa gataatcgcc
    31861 aaccctgagc aagatttacg tgctgagcaa aagaaaaaag gcagcgcgat tcaagttatt
    31921 tcgccgcaaa atgcctttat tatgacagat atcctatcaa attctgtttt aatgggaact
    31981 atggcctatg gctcatcgaa tgggaaaaaa cttgagtata gaagaagcga tggaactcgg
    32041 tatacaattc ctgcagccgg aaaaacaggt acaacacaaa actggtccga tgcttggaat
    32101 tgaataccga gttccatcgc ttcttctata c
//
LOCUS       DAOSSW010000011        27106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_1967_length_27106_cov_6.270267, whole genome shotgun sequence.
ACCESSION   DAOSSW010000011 DAOSSW010000000
VERSION     DAOSSW010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 27106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27106
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1967_length_27106_cov_6.270267"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..815)
                     /locus_tag="PKW26_01990"
     CDS             complement(159..815)
                     /locus_tag="PKW26_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92372.1"
                     /translation="MSTCPNSDGYSVYLDGEIPKQYEAQYRQHLEDCLACKKNYENMQ
                     SITRILHEDAEAIQFTKAELDESFIKLQRLVQFQENTKYTKRNDGMRTLKHALPAIAA
                     ALIIGIFIPLKFKQTESDAFTPTLHTINPTARLITTKGIIAEGSLSEALQKASYTANK
                     SSLEFNQNLVNSVDIFRPATLNEPSNIQIKIFDKTDSFQKTNSDFYQASYYTKGKFLP
                     "
     gene            complement(799..1362)
                     /locus_tag="PKW26_01995"
     CDS             complement(799..1362)
                     /locus_tag="PKW26_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOQ92373.1"
                     /translation="MNDSILKKTKETGPYRGDSQTDFKLIYDETMTLLFRIAYKVVND
                     EEIAEDMAHDSLLKLHEKAMVFPTIDDAKYWLIRVVKNASLNYVKRRGRQKIAYDKAL
                     REDTRKSESGEAILLKKETKEAVLEALDQLPEKLRAILILREYAELNYKEIGRVLGIT
                     EGNVKVRVFRARERLSKILGEDDVYMP"
     gene            complement(1407..2879)
                     /gene="cysS"
                     /locus_tag="PKW26_02000"
     CDS             complement(1407..2879)
                     /gene="cysS"
                     /locus_tag="PKW26_02000"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOQ92374.1"
                     /translation="MALRVYNTMARSVEEFLPIKKDEVGFYGCGPTVYNYAHIGNLRA
                     YVFLDLLNRTFSYLGYTVNHVMNITDIGHLTGDSDDGEDKMLKTAQEKGKTVLEIAAY
                     YTEAFFKDIDALNIKRPTVICKATDHIPEMIALIERLEKNGHTYMAGGNLYFDVSTFP
                     EYGKLARINMDDLKAGARIDIDENKKNPFDFVLWFTKSKFENQALVWDSPWGKGYPGW
                     HIECSAMSMKYLGENFDIHTGGIDHIPIHHTNEIAQSEGATGKKWVNYWLHNEFLVLS
                     KEEGKMSKSSGSFLRLSSLLEKGYEALDYRFFLLGAHYRSQLAFSWQAMDSAKQSRRA
                     LLGRIAKILETITEEKREALLQKDLSQLGEKARAHHKAFISAIEDDLSSPRCLSELQM
                     LVKNPDISNEEKLLLIQDFDTVLALDLLASAKELTEKKEDENTISSELLAELEKRIKE
                     RDEAKKEKDYARADAIRNELLANGIMLIDSPEGTSWKKQN"
     gene            2954..3997
                     /gene="murB"
                     /locus_tag="PKW26_02005"
     CDS             2954..3997
                     /gene="murB"
                     /locus_tag="PKW26_02005"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOQ92375.1"
                     /translation="MNTVCKNRENSKTKKQGMHPAFRGIIAENFNLSHLSSFRVGGDA
                     SLFIEPFSQKALFYAFDFLKSIQSPFFILGGASNLVIADEGIPIPVLSMRSLRTISLD
                     SALSSTEEKNNQAEAKALFLRCEAGASISEIIDFCIAEGLTGLEQFAGLPGSIGGAVF
                     MNARCYELSISERLSQVRFIDCEKIENTEFCNAQSLFEDYFMNEADWDYKVSPFQNTK
                     NCIVEVVLRVEKADTQKVADNAAVYIADREKKGHFKYPSAGSVFKNNRSFGKPSGKIV
                     DEAGLRGLQRGGAQVAPWHGNFIINTGSATAKDIRYLVEEVQKSVKKQFGFDLEPEII
                     FSDSLIKESSYCV"
     gene            4065..5243
                     /locus_tag="PKW26_02010"
     CDS             4065..5243
                     /locus_tag="PKW26_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOQ92376.1"
                     /translation="MLQLAFINFRKDSYIFVEGKATSDSFYIIHSGKVRCSKENGIGM
                     DTNTLLGVGDFLGVISCLSGQPQIETVVALTDVIVISVKREQFPDLIKNNTPVAMKII
                     KTFAARMRQLNETLTKLTLKSSIIDSPEQLFNIAAYYERLGKRDIAIYGYYQYMKACP
                     NGMQIEAAKMRFITLKPRSRAVYFESPPDLVRSYPKDTMIFSECQSGFDLFIIQEGQV
                     KITKMVDGNEVILAVLKKGDIFGEMALLENKPRSASAIAYDACKLMVVNSQNFNQMVT
                     TQPQLIARLTTTLADRLWAMNRQLENTQIRDPVSKLFDMLALQLEKAKIQVSSGVPHQ
                     FDITPEDLANMCGLPLDQQGPVVQSFIRDQRVKLVNNRILISDPVELIKTASYFRKVK
                     "
     gene            5245..5781
                     /locus_tag="PKW26_02015"
     CDS             5245..5781
                     /locus_tag="PKW26_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92377.1"
                     /translation="MRNKKYAFVCLLFSFSLLFFGQSISELPSGYRTIRLGMDLESVK
                     QELAADSLFNYRGERDVSLLPDQERQLIEVEGHSFIQKAWFQFIDGKLYAITLQMDET
                     KLDYGSFFKTLSEKYGKPQHLSPEQTSWENEVLRMSLERPLSLKYLDLKVYNALLDKS
                     RVDAAAFEITRELFLESF"
     gene            5805..6269
                     /locus_tag="PKW26_02020"
     CDS             5805..6269
                     /locus_tag="PKW26_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOQ92378.1"
                     /translation="MRDFRPKGKQIASLLLFLLFSLSFFSCEGETLPIHDLEIKKANG
                     DTLLLKAEIAIKEEDQMKGLMFRKSVPDGTAMLFVFDKDKIARFWMKDTPTALSIAYI
                     DYKGEIRDILDMEPLSLKEVVSTSYVRYALEVRQGWFQEKGIAVGDTISPLF"
     gene            complement(6261..7385)
                     /locus_tag="PKW26_02025"
     CDS             complement(6261..7385)
                     /locus_tag="PKW26_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ92379.1"
                     /translation="MNHPLDSIIFIQLPEDALYEDSGFHLDPSIMLPVQKPNANEDFN
                     PKALSWDGILAGILTLLAYDADNEHIDYYKSLLLHTRPNIKLELTEAAILKIKNEDYD
                     IAEEIFLSLRGLDSEDPITILNSALFYEQRAESYRNSSLHDDANAYEEKAHSYYKKAL
                     HAEPPIADAFFNAAFFYLKQKNASQAKDYFETYIALCQGEEFEQDETVQNKIEKAQEI
                     INDINSKNLDDELFKSAYDFIRLGQEEKGLEAIKQFLKKNPKVWNAWFLLGWALRRLE
                     RFSEAKEAFLQCIALDGINVDVYNECAICYMEEGSYAESKNMLYKALQLEGENTKIMS
                     NLGFLALREGNVEEARSFFLAVLEFDPDDALVLATLEGLE"
     gene            complement(7395..8354)
                     /locus_tag="PKW26_02030"
     CDS             complement(7395..8354)
                     /locus_tag="PKW26_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOQ92380.1"
                     /translation="MGILTSQQLTKYYDLYRDTEIIFTKEIIRTLNLDPRQVFIKFSG
                     SQWPCIINSTSFLAARIIIGVKGGAYAQLAKNNPTVNLRFSFIQADNQPLSFFITGRV
                     SNIERYMNSADLAVVTINYTQRPPDDLIEILGTLLEANVNAVRRKEERILINDDTKRK
                     LGLLKEETIVYIDNVPRHCILRDISFSGAKVILLGLAAFLQKKETILRLDFEDPRETI
                     SLKGSVVAVTQITGRKDMVAMSMKFDEKAVPFSYKLQINKYLTAIRKTQLSQTQAKER
                     AAQEAAKQTAIAEERARQAHTKANPADSQEEEASEQGPKHEQA"
     gene            complement(8458..8673)
                     /gene="rpsU"
                     /locus_tag="PKW26_02035"
     CDS             complement(8458..8673)
                     /gene="rpsU"
                     /locus_tag="PKW26_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOQ92381.1"
                     /translation="MARINVDDNENLEKAIKRFKRMIEKEGIIREYKKREYYEKPSTI
                     QNRKNKTLQRKLSKKTRKTYDAGKSGY"
     gene            complement(8754..9848)
                     /locus_tag="PKW26_02040"
     CDS             complement(8754..9848)
                     /locus_tag="PKW26_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92382.1"
                     /translation="MEAEKKIHIDDFTPINVEEFYDIEQSENTFSTDIVFLTEHESSL
                     IQLVSDRLKTISPETLEGIENRLTDVRRLARDIARFPSLLSKQILTSATEVQTQHTLA
                     ESLLEYREGDKTLHLPTKAILGKGFLVAKFHAFAAITKIAQHSEFPQKEIQKLNNATL
                     AILFTIMVEDVYLNLLDDLSIPIDIRRQIAYSLIILWEHRIDQTVIDVAPVLDSVWRA
                     RRKIAPAFGTMVGTSELLLLSIEMDDQWRDFILKKLSEKDVSFAMQEFLFALSHEQIQ
                     KLKTILKEQGINAINRDEVYTFLKQKPSSFYDEDPRSFYLLYSVRRENAKARKKLNAE
                     GPHNTLEDHFIRFILEQNKEKQTKDIHAYT"
     gene            9894..11090
                     /locus_tag="PKW26_02045"
     CDS             9894..11090
                     /locus_tag="PKW26_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOQ92383.1"
                     /translation="MDTRNFIKKVLFFLVFFNGLQLYAQDLSLSKDDVRLVPAEEGGY
                     HLFIRQKDGVASVLLTETTKTLDGMADNYAYRALEWNAINGDEKRMLDGAFLDSPTSR
                     YSLIDSTPEADADFGSSFHIYIPERMEFGYPWSRNGVVQIQKGTFISIRTFTKPYADY
                     EGSDFLDNPFMFNFIPKKIENEPEEEEIVLTDDYNPVAVAAFEEIAALNEGFFIYSRG
                     PESLASDVLKSVARIKNKHHVDIVFAIDTTGSMKDDILAIQEELVPLLLSAFEDFENV
                     RYGLLLYRDYVDNYRYKNLPVKFYDFTSDIEVFLDEILAITIVGNPGGDIPEAVWEAL
                     YASIEFYDWDMSAERKVILIGDAPPHPTPRGYTIRATKDLIEGLAREKNIVIDAIILP
                     DDTAKK"
     gene            complement(11142..11567)
                     /locus_tag="PKW26_02050"
     CDS             complement(11142..11567)
                     /locus_tag="PKW26_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92384.1"
                     /translation="MKHTIDLPMKHINFKQMKIKQIASLILLCLFLFSCTSVPKTAPT
                     DASVEDLTLLAQDSLDVSNYKAAEFYYQTILNRYGSDLSVAVAAEYEIAHIRIKQKKW
                     NDAKERLETILDYYDSDAAEYLAPHYKVLAEIDYAKIPK"
     gene            complement(11524..11871)
                     /locus_tag="PKW26_02055"
     CDS             complement(11524..11871)
                     /locus_tag="PKW26_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92385.1"
                     /translation="MNQKETFTETETLQENDVISEKIYCANCVHCKLVPSPAGIQDQF
                     YLRVRCAAGKWKKKLGEEKIHKYFTVTRRCIDSCDAYEPMGETEEFIKQLKKDLPSKD
                     ETYDRFTHETYQF"
     gene            12069..13121
                     /gene="argC"
                     /locus_tag="PKW26_02060"
     CDS             12069..13121
                     /gene="argC"
                     /locus_tag="PKW26_02060"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOQ92386.1"
                     /translation="MIKVGIIGATGYTGVELIAFLLQHPHVKELFLSSVSFEGQHIED
                     IYPNLRSFCNTQKCSGNLLNADEVFEKSDLIFTALPHGLAEKWADACLKEGKKLIDLS
                     SDFRFDDDEETFTKWYKKPWDFPETHRAAVYGLPELHREKIKKTSIVGNPGCYVTSAL
                     LALIPAVENDLVSTSPIIIDSKSGVTGTGRNPSAANNFSECGESFKAYGVAAHRHQPE
                     IAHVLSSIAKEKPSIVFTPHLVPMSRGILSCSYAELKKEVSIEEMYRLYTDFYKDEVF
                     IRVLPLGSMPETAHVRGTNYCDISLFVVNEGKMLQVVSVLDNMVKGASGQAIQNMNIM
                     YGFDEKAGLDLIPRAF"
     gene            13161..14420
                     /gene="argJ"
                     /locus_tag="PKW26_02065"
     CDS             13161..14420
                     /gene="argJ"
                     /locus_tag="PKW26_02065"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOQ92387.1"
                     /translation="MNTNKTSIQWAEGGVCFPKGFTAHALCAGIKKSSKKEDLALIVS
                     EVPSRASGVFTKNLVQAEPVKLSRSYLEKEKKAQAIIVNSGNANACTGSEGAANARAM
                     TEAAAKALASAGYTELSAEDVLIASTGVIGKQMPIEGILPKIPDLVSGLSKEGNELAR
                     RAIMTTDLVHKEAALSVQIAGKEVRLGTMAKGSGMIHINMGTMLGFVTSDCNITSELL
                     DEALKESVLDSYNAVSVDGDTSTNDTLFILANGLADNPCIDKKNEDYYVFLEALKAIN
                     IEMAKAIASDGEGATHMLECELRGASDKESARNLAKSVISSSLVKAAFFGRDANWGRI
                     LCALGYSGSLFSPEKVSLSFASEAGELLVFEKGRPLDFDEDKAKMILSEKNIRILVDL
                     EDGEASAKAWGCDLSYDYVKINGDYRT"
     gene            14450..15346
                     /gene="argB"
                     /locus_tag="PKW26_02070"
     CDS             14450..15346
                     /gene="argB"
                     /locus_tag="PKW26_02070"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOQ92388.1"
                     /translation="MSEKHIKESELSNKDWAEVLVEALPYFKHWVGKVVVVKYGGNAM
                     LNEELKQAVMQDIVLLNTIGIQVVLVHGGGPEINNMLEKIGKESKFVQGLRYTDEETM
                     EVVQMVLTGKLNKDIVGILLQEGGKACGLSGVDSGLLRAKKIDKNGEDLGFVGEVSEV
                     NPEILKSLLDQGFIPVVSTVALGEQGDTALYNINADTAAAKIAVALGAEKFVQLTNVP
                     GVLRDIKNPNSLIKRIAREAIPSMIATGVISDGMIPKIECCLEALKGGVPRTHIIDGR
                     VKHSLLIEMFSDRGVGTMLMNE"
     gene            15383..16534
                     /locus_tag="PKW26_02075"
     CDS             15383..16534
                     /locus_tag="PKW26_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HOQ92389.1"
                     /translation="MKERVANNYGSFPVVFTKGKGAQLRDIHGKEYIDFVSGIGVNCL
                     GHNHPALVTAIQKQVEKQIHISNYYISDVGVSYTDALLKATEMEGVFYCNSGAEANEA
                     AIKLARKYGFGIQAQKKTIVTLKQSFHGRTLTTLSATGQEKFHPESFAPYTPDFKHMK
                     ANDYAALDFIDASVCAIMIEAVQGEGGVNLIDADWAQALEKKAREVDALFIVDEVQTG
                     MMRTGSLLASTTLGINPDIITLAKGIAGGVPMGACLFRGRAKDVFVAGDHQSTFGGNP
                     LALAAAKAVLKEINTESMQNHVIKMGEKIQNTIRSWNLDIIQEVRGRGLMIGIDINFE
                     AAPFQKACLDNGLCVTTAGPKTLRFLPPLIISEAEIETGLAIFREVLTA"
     gene            16531..17832
                     /locus_tag="PKW26_02080"
     CDS             16531..17832
                     /locus_tag="PKW26_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HOQ92390.1"
                     /translation="MIEKHTKKLEPFSLPASLLLGAATAATQIEGSSIPNSWLDWAEQ
                     GKIKDGTSPLISADHWNRYEEDIALMAKMGMSIYRLGIEWSRIEPEDGVFDASAIEHY
                     RRLIKLLQKNKIQVLVTLHHFTNPLWFEKMAAFEEEKSIDIFLRFVTYVVESLKDLVD
                     EWISFNEINVYAVNGYFFGIWPPGKKNFSSLQKVYTHMTISHIKSYEKIHSIYGEKPV
                     RVGLAHHYRIFDAYHWWNPFHQLSAFLYDALFQKGLTKSCATGRRTFPMQKKAFKKHG
                     IKKGNYHDFLGINYYTRDAVRFFKQDIFPRKDYNDLGWEIYPKGLGIICKGLYKKYKK
                     PLYITENGTCDAKDQFRSLYIYEHLKVVSDLDLPIERYYHWSFMDNFEWLEGESGKFG
                     LVEIDYKSQKRTLRKSAQFYASIIKERGVSKESLSTFFPER"
     gene            complement(17794..18462)
                     /gene="eda"
                     /locus_tag="PKW26_02085"
     CDS             complement(17794..18462)
                     /gene="eda"
                     /locus_tag="PKW26_02085"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF013263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOQ92391.1"
                     /translation="MQNEILRQLKNAGVVPVVKIDFVDKAIPLAKALIAGGLSTIEIT
                     FRSDAAFGAIKALVDAKLKTQEGKPLLIGAGTVINAEIAKKAIDAGSQFLVSPGMNAK
                     TIAYCRAESTPIIPGVSSASDIEGALEQGLNLLKFFPSEALGGLKMLQALRGPFPQVS
                     FMPTGGINRANLKAYAEEKSVFAIGGSWMVAPEDIESENWEKITKLSSEALALWEEGR
                     KALL"
     gene            complement(18526..19521)
                     /locus_tag="PKW26_02090"
     CDS             complement(18526..19521)
                     /locus_tag="PKW26_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOQ92392.1"
                     /translation="MDLELLKLRSNTLNHIRSFFSEKTFLELDTPSLSRTLIPESCLE
                     VFETHFIEPFSEKQQKLYLLPSPELYIKRIIAEHRLSVFQISNCYRNVESIGRLHSPE
                     FTMLEYYSVNANYMDSLFLTEALFSSFEKNRKISSFFGNSLPDFLKPPFIQLSMNEAF
                     KSYAGFYLDDCKSSAALAEEARRLGLYESKENPLDSWQMDDLYELIFVSEVEPKLPTE
                     KPVALMDYPSFVPCLAEDKDEAHKERWELYIRGIELANCYSEERDSAKVKAYFESEGK
                     IKMQKALVKHPIDADYYKIFYNFPRCSGVALGFDRLLMLLAGKSSIDSVLPVRLF"
     gene            complement(19714..20307)
                     /locus_tag="PKW26_02095"
     CDS             complement(19714..20307)
                     /locus_tag="PKW26_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ92393.1"
                     /translation="MAIVVEHDKRKREILDKALDVFVEEGYEDVTFQKLAARCGITRT
                     TLYIYFRNKQEIFLWSIKQLTQGIEESLTEIITHDEYSSKEKLQRFCTSIVDMCLENK
                     KLFNIVLMYLMQIKKAGKDPGEKVRRRIIRLRHLLSNIIIDGIKKSEFKEISVKAAND
                     MFYSLILSTIFRLAILDQKTVEEVYDSFYLAIDGLLA"
     gene            complement(20341..21495)
                     /locus_tag="PKW26_02100"
     CDS             complement(20341..21495)
                     /locus_tag="PKW26_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOQ92394.1"
                     /translation="MHTLIALSALGAEKFLSNEIKQLGYKPLYSKSGRVAFIGDDDAL
                     FRANLCSRIADRIYLQMAAFTAKDFDALYEGIYAINWQDFFRKDVRLIIDKVRTKQSL
                     LSSEHTIQSMSHKAIYDKLGAVWKMASMPETGSISTIRIYVEKDEVLVLLDLSGEPLH
                     KRGYRNKGGPAPLRETIAASLLQAMMWKRKMPLHDAFCGSGTIPVEAALYAYNIAPGF
                     GRSFALENLSFYDQKRVDEILKKEAAKIRPDAHARITGTDIDAEAIERARLHAEHACV
                     TAGRALQLIGSDARIQRPDFEIADFRDLKAPYDEGILLSNLPFGERLMNKDEVAALYT
                     DIPKLFEAFPHWQFGFYTANPQFEAAVDKKAKQERLIKTGNLNTVFYQYE"
     gene            21618..23183
                     /gene="gltX"
                     /locus_tag="PKW26_02105"
     CDS             21618..23183
                     /gene="gltX"
                     /locus_tag="PKW26_02105"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOQ92395.1"
                     /translation="MSVRVRYAPSPTGMQHIGGVRTALFNYLFARSQGGSFILRLEDT
                     DRTRYDEAYVQNLYDTLNWLGIEWDEGGEKAGDYGPYVQSERFDLYKKAADELIAKGE
                     AYHCFCNEERLSALRTEQIEKKSSVLGYDRHCRELSDDEVKKRLASGEKSVIRLKIPL
                     TGETHFQDALLGDISWKNSDMSPDPVLLKTDGFPTYHLANVVDDHYMQISHVMRAQEW
                     IPSTPLHVRMYHSFGWEAPVFCHLPMVNGSDGQKLSKRHGATNMNEFRSRGYLPQAII
                     NYVAMLGCSYEEGRDLFPLEDLERLFKIEHLNKAGAVFDYKKLEWFNGQYIRLLSDEA
                     LLEKTLPFITGTGDALLPIHASDAFPAPHTGPEYSGISMKEDGSLIAANMNDAEIRAK
                     LLSLMPLIKERLSYLTEAAEKVAFIFKPLPEISKEALIPKKLDECKTKEVLSGARDFI
                     RELFLLDEAEQEQLPKSYAEKLGVKLGDFMMPLRIALTASKVSPPLVGSIIALGEKEA
                     LLRLEKSIALLSC"
     gene            23294..24649
                     /locus_tag="PKW26_02110"
     CDS             23294..24649
                     /locus_tag="PKW26_02110"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOQ92396.1"
                     /translation="MSERSLTMEKIVSLSKRRGFVFQSSEIYGGQTGAWDYGPLGIEL
                     KNNIQRAWWKEMTQMQDNIVGLDAAILMHPKVWEASGHVASFSDPLVDCKDCKSRFRA
                     DDDTQISLENVEKRKCPKCGGPLTEPRAFNLMFKTSIGAAEDSSSTIYLRPETAQGIY
                     VNYKNVAQSNRLKIPFGIAQVGKAFRNEIVTKNFIFRTCEFEQLEMQFFVKPGDDEKF
                     FAFWKEERMNYYPKLGIRREKLRWHEHEKLAHYAKAAFDIEYEFPMGFQELEGIHNRT
                     DFDLTQHMQYSGKDLQYIDQDNGNERYIPYIVETSAGLTRNVLMFLCDAYEEEKVADN
                     GNADDWRVVLHFHPSIAPIKVAVLPLMKKDGLAELAKDIQNELKEEFVTDYDQSGAIG
                     KRYRRQDEIGTPFCVTIDYESKEDDSVTLRYRDTMEQVRVKRSDLVEFIRKEMKSYKR
                     P"
     gene            24676..25434
                     /locus_tag="PKW26_02115"
     CDS             24676..25434
                     /locus_tag="PKW26_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ92397.1"
                     /translation="MEYVALGKTNLMVSRTSFGTGGVEDLDDEEALSIINKGYEKGIN
                     FFDLASVSLQSPLRYTFIDKRDSVILGVQTSAKNANELLEEVENFFTVFKTDYIDLLH
                     LHHLAFVPVKNDSSGLIACLERLKEEGKIKYYGFSTESLSLAEEAIESGLYATLRFPF
                     NYVSNAQEIDLLRLCKEAELGFIAYKPLLGGKIQNIPLAFGFLRQYETAVPVWGFRSE
                     DELQQIIYFEEHPPQVDSQFLQDIEHEKALFFGV"
     gene            complement(25479..26723)
                     /locus_tag="PKW26_02120"
     CDS             complement(25479..26723)
                     /locus_tag="PKW26_02120"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOQ92398.1"
                     /translation="MNSFNVVDLKENVYFTQDVVIDKTFLLLTNSIPVSNALIKALLI
                     WDFKQVYSDGTIGSGTARMDSFTPTSPQEPTDSETKKKEYIDPDILKNATEVDTSLFT
                     GEIKGDSDKSRLSVVQTIYNDYFNYINQVYTRYATHKELKLDELSDKVKEFVIFVKEN
                     RRFVLRIQPSLEAKNKNFLISHSMRSTVLAITIGLQMRLPLSKLIELGVACILHEIGM
                     IRLPPQLYMTDRPLSQNEKNTIFTHPIISYNILKENEFPLNISLGVLEHHERENGTGY
                     PRKLSGEKISTYAKIIAVACAFEAITAPRHYKEASSSHEAMIEMLKNNGQVYDPTVIK
                     ALLLSLSLYPIGGYVLLSNGKVAQVTDVNPENPQNPIVQILNEFEPSGEPKTLNTGTF
                     GLKIAQVLTKQKAMEMVKQKSS"
BASE COUNT         7874 a         4984 c         5684 g         8564 t
ORIGIN      
        1 tgcgatttgc gggctaatac cgtaaagatg tggcgccttt tgtatacctc ttgaaaaaaa
       61 aacaagattg aaaaatacaa taaaaataag cacaagaagg cttataaatc gcagcgatgg
      121 atattttcga aaaatatagc gtaatttttg tgtaaaactc aaggcaggaa tttacctttt
      181 gtataatatg atgcttgata aaaatctgaa tttgtttttt ggaaagaatc tgtcttgtca
      241 aatattttta tttgaatatt gcttggttca tttaaagttg caggtcgaaa gatatctact
      301 gagtttacca agttttgatt gaattctaag ctcgatttat ttgcagtgta ggatgctttt
      361 tgtagggctt ccgaaaggga gccttcggca atgattcctt ttgtagtgat aagtcgagct
      421 gtggggttga tcgtatgcaa ggttggggta aaagcatcgg attctgtctg tttaaacttt
      481 aagggaataa aaattccgat aatcaaggct gcagcgatgg ctggaagagc atgcttgaga
      541 gtacgcatac catcatttcg ttttgtgtat tttgtatttt cttgaaattg cacaagcctt
      601 tgcagtttta taaagctttc atcgagttct gcttttgtaa actgtattgc ttctgcatct
      661 tcgtgtagaa ttcttgttat ggactgcata ttttcgtagt tttttttgca agctaggcag
      721 tcttcgaggt gctgtcgata ttgtgcttca tactgcttgg gtatttcacc atcgaggtat
      781 actgaatagc catctgaatt agggcatgta gacatcgtct tctcctaaaa tttttgaaag
      841 cctctctctc gctcggaaaa cgcgtacttt aacgtttcct tctgttatac cgagcactct
      901 tcctatctct ttataattca attctgcgta ttctcgcaaa atcaatattg ctcgtagttt
      961 ttctggaagc tggtctagag cttccaaaac agcctctttt gtttcttttt taagtaaaat
     1021 agcttctcca ctttctgatt tccgtgtatc ctcccgcaaa gccttgtcgt aagcaatctt
     1081 ttgtctacct cgtcgtttta cataatttaa agaagcattt tttactacac gaataagcca
     1141 gtattttgca tcatctatcg ttggaaagac cattgctttt tcatgtagtt ttaacaaaga
     1201 atcatgtgcc atatcctcag caatttcttc atcgtttaca actttatatg caattctaaa
     1261 tagcaatgtc atagtttcat cgtaaatcag tttaaaatca gtctgagaat cgcccctata
     1321 gggaccggtc tcctttgttt tttttaatat agaatcgttc atagtagata acaataaagc
     1381 tcctcaaatg ttacaaaaaa ggaacattaa ttttgttttt tccaacttgt tccctcaggg
     1441 ctgtctatga gcattatacc atttgctaac aattcgtttc gtattgcatc agctcgtgca
     1501 taatcttttt cttttttcgc ctcgtctctt tcttttatgc gtttttcaag ttctgccagt
     1561 agctcgctac ttattgtatt ctcatcttct tttttctctg ttagctcttt tgcagatgca
     1621 agcaagtcga gagcaagcac tgtatcgaag tcttggatga gaaggagctt ttcttcattt
     1681 gaaatatctg ggtttttcac gagcatttgt agctcagaaa ggcatcgtgg gctcgacaaa
     1741 tcgtcttcga ttgctgaaat aaaggccttg tggtgtgctc tcgctttttc tccaagttgt
     1801 gagaggtctt tttgtaaaag cgcttcacgt ttttcttctg taatagtttc tagaattttt
     1861 gcaattctgc caaggagggc tcttctcgat tgttttgcgc tatccatcgc ttgccaagaa
     1921 aaagcgagtt ggcttcggta atgtgcgccg agtagaaaaa atcgatagtc aagggcttca
     1981 taaccttttt ccaagaggct tgaaagtctt agaaagcttc cagaggattt tgacattttt
     2041 ccttcttctt tcgataaaac aaggaattcg ttatgaagcc agtagtttac ccattttttc
     2101 cctgtggctc cttcggattg ggctatttca tttgtatggt ggatgggtat gtggtcaatg
     2161 ccaccagtgt gaatgtcaaa gttttctccc aaatatttca tgctcatagc agagcattcg
     2221 atatgccaac cgggatatcc ttttccccaa ggagagtccc aaacgagggc ttggttttca
     2281 aacttacttt ttgtaaacca aagcacaaag tcaaagggat tttttttgtt ttcgtctata
     2341 tcgattcttg ctcctgcttt taagtcatcc atgttgatgc gagcgagttt gccgtattcg
     2401 gggaaggtgc ttacgtcaaa atagagatta ccacctgcca tgtaggtatg accgtttttt
     2461 tcgagccgtt ctatgagcgc aatcatttct ggaatatggt ctgttgcctt gcaaattacc
     2521 gtcggtcgct tgatattgag tgcatctatg tctttgaaaa aggcctctgt atagtatgca
     2581 gctatttcca aaactgtttt tcccttttct tgggctgttt ttaacatttt gtcctcgcca
     2641 tcatcactgt caccagtaag atgcccgata tcagtaatat tcataacatg attgacggta
     2701 tagccgaggt atgaaaaggt tctatttaaa aggtcgagaa agacataggc gcgaagattt
     2761 cctatatggg cgtagttata gactgttgga ccacagccgt aaaagccaac ctcatctttt
     2821 tttataggca agaactcttc cacggagcgt gccattgtat tgtatacgcg taaagccatt
     2881 gatttctccc attttatcat tcattttttt agaattactc gattaaaatc gtattatacg
     2941 atataattag catatgaata ctgtatgcaa aaacagagaa aatagcaaga caaaaaagca
     3001 gggaatgcat cctgcctttc gcggtataat tgcagaaaat tttaatcttt cgcatctttc
     3061 aagttttcgg gtaggtggcg atgcaagcct ctttatagaa cctttttcac aaaaggcttt
     3121 gttttatgct tttgattttt tgaaatcaat acaaagtcct ttttttattc ttggtggtgc
     3181 gtcaaacctt gtgattgcag atgaggggat tcctattccg gtgctttcta tgcgttcttt
     3241 gcgaactatc agccttgaca gtgccttgtc gagtacagag gaaaaaaaca atcaggctga
     3301 agcaaaggcg ctctttttgc gttgtgaggc tggagcctct atttctgaaa ttatagattt
     3361 ttgcattgca gaaggattaa caggtttgga gcagtttgca ggtttaccgg ggagcatcgg
     3421 tggggcggtt tttatgaatg ctcgctgcta tgagctttct atctccgagc gactttcgca
     3481 ggtacgcttt atcgattgcg agaagataga aaatacagaa ttttgcaatg cacagagcct
     3541 ttttgaagac tactttatga atgaggctga ttgggattat aaagtctctc cctttcaaaa
     3601 tacaaaaaac tgtattgtgg aagtggtttt gcgagtagaa aaagccgata ctcaaaaggt
     3661 agctgataat gccgcggtat atatagccga tagagaaaaa aaagggcatt ttaagtatcc
     3721 ttctgcaggc tctgttttta aaaacaatcg aagttttggg aaaccaagcg gaaaaatagt
     3781 tgatgaggct gggctccgtg gtttacaaag aggaggtgcg caagttgcgc cctggcacgg
     3841 taattttata ataaatacgg gatctgcgac agcaaaagac attcggtatt tagtagaaga
     3901 ggtgcaaaaa tcagtaaaaa aacaatttgg ctttgattta gagcctgaaa ttattttttc
     3961 tgatagtctc attaaagaga gctcttattg tgtatgagcg aacaaacaat gagaattgac
     4021 agtatggcgc ttgcacctta tactcaagat atagtgaggg gaatgtgtta caactagcat
     4081 ttattaattt tagaaaagac tcgtacattt ttgttgaagg aaaggctacg agcgatagct
     4141 tttatattat tcacagtggt aaagtgcgtt gctcaaaaga aaacggtatc ggaatggata
     4201 cgaacaccct ccttggcgtt ggtgactttc ttggtgttat ttcttgtctt tcagggcaac
     4261 cacaaattga aaccgttgta gcattgacag atgttattgt tatatcggtt aaaagagaac
     4321 aattccccga cttaatcaaa aataatacgc ctgttgctat gaagattata aaaacttttg
     4381 cagctcgtat gcgccagttg aatgagacgc ttacaaaact cactttgaaa agctccatta
     4441 tcgattctcc tgagcaatta ttcaatattg ctgcctatta tgagcgcctt ggaaagcgag
     4501 acattgccat ctatggctac tatcaatata tgaaggcttg tccaaatggt atgcaaatag
     4561 aagcggccaa aatgcgtttt attacattaa aaccacgttc ccgggcggtc tactttgaat
     4621 ctcctcccga cttagtgcga agctacccaa aagatacaat gattttttct gagtgtcaaa
     4681 gtggttttga cttgtttatt atccaagaag ggcaggtgaa gattacaaaa atggttgatg
     4741 ggaatgaggt tattcttgcg gttttgaaaa aaggcgatat ttttggtgag atggccttgc
     4801 ttgaaaacaa accgagaagt gcgagtgcta ttgcctatga tgcgtgtaag ctcatggttg
     4861 taaatagcca aaactttaat caaatggtta caacgcaacc acaactcatt gcacgcttga
     4921 cgacaactct tgcagatcga ctctgggcaa tgaatcgcca gcttgaaaat acacaaatac
     4981 gtgaccctgt ttcaaaactt tttgacatgc ttgccttgca gttggaaaaa gcaaagattc
     5041 aagtgagctc tggtgtgcca catcagtttg atataacacc agaagacttg gcaaatatgt
     5101 gtggtcttcc gcttgatcag caaggtcccg ttgtgcagtc ttttattcga gaccaaagag
     5161 tgaaactcgt aaacaatcga atattgattt cagatcctgt cgaattgata aaaacagcat
     5221 cgtattttag aaaggtaaaa tagcatgcga aataaaaaat atgcctttgt ttgccttctt
     5281 ttttctttta gccttctttt ttttggacaa tctatttcgg aacttccttc tggatacagg
     5341 acaattcgat tgggcatgga cttggagtca gtaaagcaag aacttgcagc agattctctt
     5401 tttaattatc gcggagagcg agatgtttct ttgttgcccg atcaagaacg gcagctcata
     5461 gaagtagaag gtcattcatt tatacaaaag gcatggtttc agtttatcga tggaaagctc
     5521 tatgctatta cgcttcaaat ggatgagaca aagctagact acggcagttt tttcaaaacc
     5581 ttatctgaaa aatatgggaa gccgcagcat ctctctcctg agcaaacgag ctgggaaaat
     5641 gaagttctgc gtatgagctt ggagcgcccc ttatctctta agtatctcga tttaaaagtg
     5701 tataatgcgc ttttggataa gtcccgcgta gatgcggctg cttttgaaat aacgcgtgaa
     5761 ttatttttgg agtcttttta atgttttttt tcaagaatct ttgtatgagg gattttcgac
     5821 ctaagggaaa acaaatagct tctttattgc tttttctcct attttcgctt tctttttttt
     5881 cttgtgaggg ggaaaccctt ccaatacacg acttggaaat aaaaaaagca aatggcgaca
     5941 cacttcttct aaaagctgaa attgctataa aagaggaaga tcaaatgaag gggcttatgt
     6001 ttcggaaatc ggttcctgat gggactgcta tgctttttgt ttttgataaa gataaaattg
     6061 cacgcttttg gatgaaagac actccgacag cgctttctat tgcatatatc gactacaagg
     6121 gagaaatacg cgatatactt gatatggagc ccttgagtct taaagaagtg gtcagtacga
     6181 gctatgtacg ctatgccctt gaagttcgcc agggttggtt tcaagaaaag ggcattgcgg
     6241 ttggcgatac tatttcgccc ttattctaag ccttcgagtg tagccaagac aagtgcatca
     6301 tcgggatcaa attccaaaac agcgaggaaa aaagagcgag cttcttcgac atttccctct
     6361 cgtagtgcta aaaaaccgag gttagacata atctttgtgt tttctccctc aagttgtagg
     6421 gccttataca gcatattttt gctttctgca taagagcctt cttccatata gcaaatagcg
     6481 cattcgttgt atacgtcaac attgataccg tccaaggcga tacattgtaa aaaagcttct
     6541 tttgcctcag agaatcgttc gagccttcgc aaagcccatc caagcaaaaa ccatgcattc
     6601 catacttttg gatttttctt caaaaattgt tttatagctt ctaagccttt ttcttcttgc
     6661 cccaagcgta tgaaatcgta ggctgattta aacagctcat cgtcaaggtt tttgctattt
     6721 atatcgttga taatttcctg ggctttttct attttgtttt gtactgtttc atcttgctcg
     6781 aattcctctc cttgacagag agcaatatag gtttcaaaat aatcctttgc ttggcttgcg
     6841 tttttttgct tgagataaaa aaaagctgca ttaaaaaaag catcagcgat cggcggttcg
     6901 gcatgtaagg cttttttata gtagctatgt gctttttctt cataggcatt agcatcatca
     6961 tgcaagcttg aatttctata ggactcagct ctttgttcat aaaaaagtgc tgagttcaaa
     7021 atcgttatgg ggtcttcgga gtcaagtccg cgcaaagata aaaatatttc ttctgcaatg
     7081 tcatagtctt cgttttttat cttaagaata gcagcctctg ttaattcgag cttaatattt
     7141 ggtcgtgtat gtagcaaaag agatttataa tagtcaatat gctcgttatc agcatcgtag
     7201 gctaaaagag tgagaatgcc cgctaaaatg ccgtcccagg aaagagcctt gggattaaaa
     7261 tcctcgtttg catttggttt ttgtacgggg agcatgatcg agggatcgag atgaaaacca
     7321 gagtcttcgt agagtgcatc ttctggtagt tgaataaaaa taattgagtc gagagggtga
     7381 ttcattatac ttccttatgc ttgttcgtgt ttaggacctt gttcgctcgc ttcttcttct
     7441 tgagaatctg ctggatttgc ctttgtatgt gcttgtcgcg ctctttcttc agcaatagcg
     7501 gtttgttttg cagcttcctg agcagctctt tcttttgctt gcgtttgact tagttgtgtt
     7561 ttacgaattg cagttagata tttatttatt tgcagtttat atgaaaaagg aacagccttt
     7621 tcatcaaatt tcattgacat ggcaaccata tcttttctac ccgtaatttg tgttacggca
     7681 acaacgcttc cttttaaact gatagtctct cgtggatctt caaagtctaa acgcagtatg
     7741 gtttcttttt tttgtaaaaa agctgctaaa ccaagtaaga taaccttcgc acctgagaaa
     7801 gaaatatccc gtaaaataca atgccgagga acattgtcga tatatacgat tgtttcttct
     7861 ttaagaagcc cgagttttcg cttagtatcg tcatttatga gaatacgttc ttcttttctg
     7921 cgtactgcgt ttacatttgc ttctaagagt gtgccaagaa tctcgattaa atcatctggt
     7981 ggtctttgcg tataatttat ggtaacaaca gcaaggtcag ctgaattcat gtaacgttca
     8041 atattgctaa ctcgtcctgt aataaaaaag cttaagggct gattatcggc ttggataaaa
     8101 ctaaaacgca agtttactgt gggattgttt ttagcaagtt gtgcataagc accacctttt
     8161 acccctataa taatacgagc tgctaaaaat gaagtagagt ttattataca aggccattga
     8221 cttccagaga atttaataaa aacttgtctt ggatcgaggt ttaatgtgcg aatgatttct
     8281 tttgtaaaga tgatttcagt atcacgatat aaatcataat atttcgtcaa ctgttgactt
     8341 gtaagaatac ccatacattc ataataagtg aaaagtgagt cttcgtcaat gtcgaagaag
     8401 tgtagaaaag gggataaaaa aaacccaagc ctaactcggg tttttttatg ctaaaaatta
     8461 ataaccagat ttacctgcgt catatgtttt tcgtgttttt tttgataact ttctttgtaa
     8521 agttttattt tttctgtttt gaatggttga aggtttttca tagtattcgc gttttttgta
     8581 ttcgcggata attccttctt tttctatcat acgcttaaat cgtttaatag ctttttcaag
     8641 gttttcattg tcatctacat tgatacgtgc cataatttca cctccttcga ccatttcgtt
     8701 agattttata attatcatgt attataaaaa aagagtcaat gccttccttc aatttaggta
     8761 taggcgtgaa tatcttttgt ttgtttttct ttattttgtt cgaggataaa gcggataaaa
     8821 tggtcctcaa gggtgttgtg aggtccttca gcattgagtt tttttcttgc ttttgcgttt
     8881 tctcttctca ctgaatacaa taagtaaaaa cttcgtggat cttcgtcgta aaagcttgaa
     8941 ggcttttgct ttaaaaaagt atacacttca tcgcgattta tagcatttat cccttgctct
     9001 tttaagattg tttttaattt ttgaatttgt tcatgagaga gtgcaaataa aaattcttgc
     9061 attgcaaaag atacatcttt ttctgatagt ttttttagaa taaagtctcg ccattggtca
     9121 tccatttcga ttgacaagag gagaagttcg cttgtgccga ccattgtgcc aaaggcagga
     9181 gctatttttc tacgagctct ccaaacagag tcgagaacgg gagccacatc tataaccgtt
     9241 tggtcaatgc ggtgttccca aaggataatg agcgaatagg caatttggcg gcgaatgtcg
     9301 atgggtatac tgaggtcatc gaggagattg agatagacgt cttctaccat aatggtgaaa
     9361 agaatcgcta aggttgcgtt gttgagtttt tgaatttctt tttgaggaaa ctctgaatgt
     9421 tgtgcaattt tagtaatagc tgcaaaggcg tgaaattttg caacaaggaa gcctttgcct
     9481 aaaatagcct tggttggaag atgcagtgtt ttatctcctt cgcgatactc aagcaaggat
     9541 tcagctaatg tatgttgtgt ttgtacctct gttgccgatg tgagaatttg ttttgacaag
     9601 agggagggga agcgtgcgat atctcgagca agacgtcgta catccgtaag acgattttct
     9661 atgccttcaa gtgtttctgg agaaattgtt tttagtctat ctgagacaag ctggattaaa
     9721 gatgattcat gctctgttaa aaaaacaata tctgttgaga atgtattttc tgactgttct
     9781 atatcataaa attcttcaac attgatagga gtaaaatcat ctatatgtat ctttttttca
     9841 gcttccacgt gctaagtata acataaaaat aaaaaaaagc cgatataaaa aatatggata
     9901 cacgtaattt tataaaaaaa gtgctttttt ttctcgtttt ttttaatggc ctacagcttt
     9961 atgcacaaga tttgagcctt tcaaaagatg atgtacgact tgttcctgca gaagagggag
    10021 gctatcattt atttattagg caaaaagatg gtgtcgcctc ggttttattg actgaaacaa
    10081 caaaaactct cgatggaatg gctgacaatt atgcctaccg agccttggaa tggaatgcga
    10141 ttaatggcga tgaaaagcgt atgctcgatg gggctttttt agattcacct acaagtcgct
    10201 atagtttaat tgattcaaca ccggaagcag atgctgactt tggctcttct tttcatatct
    10261 atattcctga gcgtatggag tttggctatc cctggtctcg aaacggtgtg gtgcaaattc
    10321 aaaagggaac ttttataagt attcgcacct ttacaaagcc ctatgctgac tatgagggtt
    10381 cagatttttt ggataatcct tttatgttta actttattcc aaaaaaaatt gaaaatgagc
    10441 ctgaagaaga agagattgtt ttaactgatg attataatcc tgttgcggtg gctgcctttg
    10501 aggaaatagc agcattaaac gagggttttt ttatctattc tcgtggtcct gaaagcctcg
    10561 ctagtgatgt gcttaaatct gtagcgcgta ttaaaaacaa acatcatgtg gacattgttt
    10621 ttgcaattga tacaacggga agcatgaagg acgatattct tgcaatacaa gaagaacttg
    10681 tgccactatt gcttagcgct tttgaagact ttgaaaatgt acgatacggg cttttattgt
    10741 atcgcgacta tgtagacaat tataggtata aaaatcttcc cgtaaaattt tatgatttta
    10801 cgtccgatat agaagttttt ttagatgaaa tccttgcaat aactattgtt gggaatcccg
    10861 gtggcgatat tcctgaggcg gtttgggaag ccttatacgc cagtatcgaa ttttatgact
    10921 gggatatgag cgcggagcga aaagtgattc tcataggaga tgctcctcca catccaacgc
    10981 cgcgtggtta taccattcgc gcaacgaagg acttgatcga agggcttgca agggaaaaaa
    11041 acatagtaat cgatgctatt atcttacctg atgatactgc aaaaaaataa aaaaaaagac
    11101 cacttattgc tgtggtcttt ctttttacgc tctctgttta cttattttgg gatttttgca
    11161 tagtcaattt ctgcaagcac cttgtagtgc ggagctaaat attctgccgc atcactgtcg
    11221 taataatcaa gtatcgtttc gagtctttct tttgcatcgt tccatttttt ttgtttaatg
    11281 cgaatgtgag ctatctcgta ttcggcagca acggctactg aaagatcgga gccataacgg
    11341 tttagaattg tttggtagta aaattctgcg gctttgtaat ttgacacatc gagactatct
    11401 tgagcaagca aggtgaggtc ttcaacactt gcatccgtgg gcgccgtttt gggaacactt
    11461 gtgcaagaaa aaagaaagag gcaaagaagt ataagacttg ctatctgttt tattttcatt
    11521 tgtttaaaat tgatatgttt catgggtaaa tcgatcgtat gtttcatctt ttgagggtaa
    11581 atcctttttt agttgtttaa taaactcttc tgtttctccc atgggctcat aggcatcaca
    11641 agagtcaatg catctacggg ttaccgtaaa atacttgtga attttttctt cgcccagttt
    11701 tttcttccat tttcctgctg cacaacgtac tcgcaggtaa aattggtctt gaatgccagc
    11761 gggacttggc actaacttac aatgcacaca gtttgcacaa taaatttttt cacttattac
    11821 atcgttttct tgcaatgttt cagtttctgt gaaagtctct ttttgattca taatgctctc
    11881 catacaattt taattaatac aacgtatacg tattatttta ccacgcacta tctgtatcgt
    11941 caagttttct agctagatta tcggtttatt tcttgagaaa gcatagcttt tctactttat
    12001 tttctctctc cttgttattt tcaagcctaa tcgatatact gtagggactt tgacaatggg
    12061 agaaatcgat gataaaagtt ggtattattg gtgcaactgg gtatacgggt gtagagttaa
    12121 ttgcgttttt actacaacat cctcatgtaa aagagctttt tttaagctcg gttagctttg
    12181 aaggacaaca tattgaagat atctatccaa atcttcgctc tttttgcaat acgcaaaaat
    12241 gttcgggtaa cttgctcaat gctgacgagg tttttgaaaa atcggatctc atttttaccg
    12301 cccttcctca cggtcttgca gaaaaatggg cagatgcctg tttaaaagag ggtaaaaaac
    12361 tgatagatct ttcgagtgat ttccgctttg acgatgacga ggaaaccttt accaagtggt
    12421 ataagaaacc ttgggatttt cctgaaacgc atagggctgc tgtctatggc ttgccagaat
    12481 tgcatcgtga aaaaataaaa aaaacaagca ttgtgggaaa tcctggttgc tatgttacta
    12541 gtgccttgct tgctctcatt cctgcggtag aaaatgacct tgtatcaact tctcctataa
    12601 tcattgacag taaaagcggt gtaacaggaa caggacgaaa tccaagcgct gcaaataatt
    12661 tttctgaatg cggtgagtct ttcaaagctt atggggtagc tgcgcatcgg catcaaccag
    12721 aaatagcgca tgtcctttca tcgattgcaa aagaaaaacc gtctattgtt tttactccac
    12781 atcttgttcc catgagtcgc ggcattcttt catgctccta tgccgagctt aaaaaggaag
    12841 tttcaataga agaaatgtat cgcttgtata cagactttta taaagatgaa gtctttattc
    12901 gcgttcttcc cttgggaagc atgcctgaaa cagctcatgt gcgcggaaca aattattgtg
    12961 atatttcact ctttgtcgta aacgagggga agatgctgca agtagtatcg gttcttgaca
    13021 atatggtaaa aggtgcttcg gggcaagcaa ttcaaaacat gaatattatg tatggctttg
    13081 atgaaaaagc tggtctcgac cttatccccc gagcttttta gtttttagca tagatcaaaa
    13141 taggtaaaag aggtagcaag atgaatacaa ataaaacatc tatacaatgg gctgaaggtg
    13201 gcgtctgctt tcccaaaggc tttacagcgc atgccctctg tgcagggata aaaaaatcga
    13261 gcaaaaaaga agacctcgct cttattgttt cagaagtccc ttctcgggca agtggcgttt
    13321 ttacaaaaaa tctcgttcaa gctgagccgg taaaattgag ccgttcatat ctagaaaaag
    13381 aaaaaaaagc tcaggcaatt attgtcaatt cgggtaatgc aaatgcctgt accggtagcg
    13441 aaggggcagc aaatgcgcgt gctatgacag aggctgcagc aaaggctttg gcgagtgctg
    13501 gttataccga gctttctgca gaagatgttc ttattgcatc tactggcgtt ataggaaaac
    13561 aaatgcccat agaaggtatc ttgcccaaaa tccccgacct tgtttcgggc ttgtccaagg
    13621 agggcaacga gcttgcacga cgggcgatta tgacaaccga tcttgtgcac aaagaagccg
    13681 cgctttctgt gcaaattgcg ggcaaagagg ttcgactcgg tacaatggca aagggttccg
    13741 gcatgataca tatcaatatg ggaacgatgc ttggctttgt tacaagcgat tgcaatatta
    13801 catcggaact tctcgatgag gcgctcaaag aaagcgtcct cgattcttat aatgcggtct
    13861 cagttgatgg ggatacaagc acaaatgaca cgctctttat tctggcaaat ggtttggctg
    13921 ataatccttg tatcgataaa aaaaacgaag actactatgt ttttctagaa gctttgaagg
    13981 ctattaatat agaaatggca aaggcaattg cttccgatgg tgagggtgca acgcatatgc
    14041 tcgaatgtga gctacgtggt gcaagcgaca aggaaagcgc tcgtaacttg gcaaagtcgg
    14101 ttatttcttc aagcttggta aaagccgcat tttttggacg ggatgcaaac tgggggagga
    14161 ttctttgtgc cctcggttat tctggctctc tttttagccc agaaaaagta agcctttcct
    14221 ttgcaagcga ggcaggtgaa ttgcttgtct ttgaaaaagg taggccacta gactttgacg
    14281 aggataaggc aaagatgatt ttaagtgaaa aaaacattcg tattcttgtt gatttagaag
    14341 atggagaagc gagtgcaaag gcctggggct gtgatttaag ctatgattat gtaaaaataa
    14401 atggtgatta tcgtacctaa gcaataggga aaggctatga ggaataaaaa tgagtgaaaa
    14461 gcacataaaa gaatcagaat tgagcaataa ggactgggca gaagttttag ttgaagccct
    14521 tccgtatttt aagcattggg ttggaaaagt cgttgttgta aaatacggcg gaaacgctat
    14581 gttaaacgag gaattaaaac aagcagttat gcaagacatt gttttattga ataccattgg
    14641 tatccaggta gtcctcgttc acggcggcgg tcctgaaata aacaatatgc ttgaaaaaat
    14701 aggtaaggaa tcaaagtttg tgcagggttt gcgctatacc gatgaagaaa ccatggaagt
    14761 ggtgcaaatg gtattgacgg gaaaacttaa taaggacatt gtcggcattc ttttgcaaga
    14821 aggtggaaag gcatgtggtt taagcggcgt tgattcaggc ttattgcgag caaaaaaaat
    14881 agataaaaac ggtgaagatt taggctttgt tggagaagtg agtgaggtaa atcctgaaat
    14941 tcttaaatcc cttttggacc aaggctttat tcccgttgtt tcaactgttg cccttggtga
    15001 gcaaggagac acagcgctct acaatataaa tgcagatact gctgcggcaa aaattgcggt
    15061 tgccttggga gcagaaaaat ttgtgcagtt aacaaatgtg ccaggagtac ttcgcgatat
    15121 taaaaacccc aactctctca taaagcgtat cgcccgggaa gcaattcctt cgatgatcgc
    15181 tacgggagta atatctgatg gaatgatacc gaaaattgaa tgttgcttag aggcattgaa
    15241 aggcggtgtt ccacggacgc atattattga cgggagggta aaacattcac tgcttataga
    15301 aatgttttca gatcgcggtg ttggaacaat gttgatgaat gagtagtata aaactgagtc
    15361 tttattattt gatggaggaa gcatgaaaga acgagttgca aataattatg gtagttttcc
    15421 tgttgttttt acaaagggga agggtgcaca actaagagat attcacggaa aagaatacat
    15481 agactttgtt tcgggcattg gcgttaattg cttaggacat aatcatccag cattggttac
    15541 tgcaattcaa aaacaagtag aaaaacaaat ccatatatca aattattata ttagcgatgt
    15601 tggtgtttca tacaccgatg cccttttaaa agcgacagag atggaaggcg ttttttattg
    15661 taattccggg gctgaggcaa atgaggccgc tataaaacta gctcgaaaat atggctttgg
    15721 catacaagca caaaaaaaga ccattgtaac tttaaagcag tcttttcatg gacggaccct
    15781 aacaacgctt tcggcaaccg ggcaagaaaa atttcatcct gaaagttttg caccctatac
    15841 tccagatttt aagcacatga aggcaaacga ttatgctgct cttgatttta tcgatgcatc
    15901 tgtttgtgca attatgattg aagctgttca gggcgaaggt ggggtaaacc tcatcgatgc
    15961 agactgggca caagcgcttg aaaaaaaagc tcgtgaagtc gatgctctct ttattgtcga
    16021 tgaagtgcaa acgggtatga tgcgaacggg aagtctcctg gcaagtacca cactgggaat
    16081 aaatccagat attataacgc ttgcaaaggg gattgctggc ggcgtaccaa tgggagcctg
    16141 tcttttccga ggaagggcaa aggatgtctt tgttgcagga gaccatcagt caacctttgg
    16201 gggaaatcca ctcgcacttg ctgctgcaaa agcggtttta aaagaaatca atactgagtc
    16261 tatgcaaaat catgttataa aaatgggaga gaagattcaa aacacaattc gctcttggaa
    16321 tctcgatatt attcaagaag tgcggggaag ggggcttatg ataggaatcg atattaactt
    16381 cgaagcagcg ccttttcaaa aagcctgcct cgataatggg ctctgtgtta cgactgctgg
    16441 tcctaaaaca cttcgttttt tgccaccgct tattattagt gaggcagaaa tagaaaccgg
    16501 tcttgctatt tttcgggaag tgcttactgc atgatagaaa aacatacaaa aaagcttgaa
    16561 ccattttctc tgcccgcgtc cctacttctt ggagcagcaa ctgcagcaac acaaatcgag
    16621 ggctcatcta taccgaattc atggcttgat tgggcagagc aggggaagat aaaagacggc
    16681 acatctccct tgattagcgc tgaccattgg aaccgctacg aagaagacat tgcacttatg
    16741 gcaaaaatgg gcatgtccat atatcggctc ggtattgaat ggagtcgtat tgaacctgag
    16801 gatggtgttt ttgacgcaag cgctatcgag cattatagaa ggctcataaa gcttttgcaa
    16861 aaaaataaaa tacaagtctt ggttaccttg catcatttta ccaatccact gtggtttgaa
    16921 aaaatggctg cctttgaaga agaaaaatct atcgacattt ttttacgctt tgtaacgtat
    16981 gttgttgaat ccttaaaaga ccttgttgat gaatggatta gttttaacga aataaatgtc
    17041 tatgcggtaa acggctattt ttttggtata tggccaccag gtaaaaaaaa ttttagctca
    17101 ttgcaaaaag tgtataccca catgacaatc agtcatatta agtcttatga aaaaattcat
    17161 agcatctatg gcgaaaaacc agtgcgcgtg ggactcgctc atcattaccg cattttcgat
    17221 gcctatcact ggtggaatcc cttccatcaa ttgagtgctt ttttgtacga cgctcttttt
    17281 caaaagggtt taacaaaaag ctgtgcgacg gggaggcgaa cctttcccat gcaaaaaaag
    17341 gcatttaaaa agcatggtat aaaaaaaggc aactaccacg attttttggg tattaattat
    17401 tatacgcgag atgcggtgcg cttttttaaa caagatatct ttcctagaaa agactataac
    17461 gacctcggct gggagatata tcccaagggc ttaggaatta tttgcaaagg gctttataaa
    17521 aaatacaaaa agcctctgta tataacagaa aacggtacct gcgatgccaa agaccagttc
    17581 cgtagcctct atatctatga gcatttaaaa gtcgtttcag acctagactt gccaatagag
    17641 cgctattatc actggtcctt catggacaat tttgaatggc tcgaaggcga aagcggtaag
    17701 tttgggcttg tagaaattga ttataagagc caaaaaagaa ccttgagaaa aagtgctcag
    17761 ttttatgcga gcattataaa agagcgaggc gtatcaaagg agagcctttc taccttcttc
    17821 ccagagcgct aatgcttctg agctaagctt tgttattttt tcccagtttt cgctttctat
    17881 atcttctgga gccaccatcc agctacctcc aatggcaaaa accgattttt cctctgcata
    17941 ggcttttaag tttgctcgat tgatgccacc tgttggcata aaagaaacct gcggaaaggg
    18001 gccacgaagt gcttgaagca tcttcaaacc gccgagggct tcgcttggga aaaactttag
    18061 aagattgagc ccctgctcaa gagctccttc gatatcgctt gccgagctta ctcccgggat
    18121 aatgggagtg gactctgccc tgcaataggc aatggttttt gcattcattc cggggcttac
    18181 gaggaattga cttccagcat ctatggcttt ttttgcaatc tctgcattga taactgtacc
    18241 tgcaccgatg agaagaggtt ttccctcctg cgttttgagc tttgcatcaa cgagtgcctt
    18301 gattgcccca aaggcagcat cgctacgaaa ggtaatttct atagtcgata aaccaccggc
    18361 gatcaatgcc ttggcaaggg gaatcgcctt atctacaaaa tctattttta caacaggtac
    18421 gacgcctgca ttttttaatt gccttagaat ttcattttgc attttattac ctcatttttt
    18481 attagctacg atacagtata gcatagattt ttttgctaaa gctagttaaa aaagtcgaac
    18541 aggcaaaaca gaatcgatac ttgattttcc tgctaaaagc atgagtaagc gatcaaagcc
    18601 aagggcaact ccagagcagc gaggaaaatt ataaaaaatt ttatagtaat ccgcatctat
    18661 gggatgtttt acaagggcct tttgcatttt gatttttcct tcactttcaa agtaggcttt
    18721 aacctttgcg ctatcccgtt cttcgctata acaatttgca agttctatgc cccttatata
    18781 cagctcccat ctttccttat gtgcctcatc cttgtcttct gcgagacagg gcacaaagga
    18841 tggataatcc ataagagcga caggtttttc agtcggaagc ttgggttcta cctccgagac
    18901 aaagatgagt tcgtagaggt catccatttg ccagctatct aggggatttt cctttgattc
    18961 atagagccca agacggcgag cttcttccgc aagagcggca ctcgatttac aatcatcgag
    19021 ataaaaacct gcataggact taaaagcttc gttcatactt agttggataa aaggcggttt
    19081 tagaaaatct ggtagactat tgccaaaaaa agaagagatt ttcctgtttt tttcaaagga
    19141 tgaaaaaagc gcttcagtta gaaagagcga gtccatataa ttagcattta cagaataata
    19201 ctcgagcatg gtaaattcag ggctatgaag cctaccaatc gattctacat tgcggtagca
    19261 attagaaatt tgaaaaacag aaagcctatg ttctgcgata atgcgcttga tataaagctc
    19321 cggggagggg agaaggtaga gtttttgctg cttttcacta aaaggctcga taaaatgtgt
    19381 ttcaaatact tcaagacagc tttctggaat aagtgttcga ctgaggctcg gagtgtcaag
    19441 ctctaaaaaa gttttttctg aaaaaaaaga gcgaatatga ttgagcgtat tgcttcgcag
    19501 ttttaaaagt tctaaatcca tagcctgcat actactataa aaaagctttt aaatacagaa
    19561 gtaaaaaaaa ccgcccctta gaaactctaa aaggcggtaa aaaggagagg agaattatga
    19621 aatgacactg catgttgttg tatcaagttc tgctaataca acagtcaata tgagaaaagg
    19681 ttacaggatt atttgttaaa aaattgaaat gtgctaggct aaaagtccat cgatagccag
    19741 ataaaaagaa tcgtagactt cttctactgt tttttggtcg agaattgcta gacggaaaat
    19801 agtcgaaagg atgaggctgt agaacatatc gtttgccgct tttacactta tttctttgaa
    19861 ttcacttttt tttatcccat cgataatgat atttgaaagc aaatgcctca agcgaataat
    19921 tctccgcctc actttttcgc cagggtcttt tccggctttt tttatttgca tcagatacat
    19981 tagtacaatg ttaaagagtt ttttgttttc aagacacatg tcgacaatgc tcgtgcaaaa
    20041 tctttgtagt ttttctttgc tagaatattc atcatgggta ataatttctg tgagcgattc
    20101 ttctataccc tgtgttaatt gcttaatact ccataaaaaa atttcttgct tatttctaaa
    20161 ataaatatag agagttgttc ttgtaatgcc acagcgagct gccagttttt gaaatgttac
    20221 atcttcataa ccttcttcta caaatacatc gagcgctttg tccaaaattt ctcttttgcg
    20281 tttatcgtgc tcaactacaa ttgccattac aatctccttt ttttaaaatt tacttataat
    20341 ttactcatat tgatagaata ccgtatttaa gtttcctgtt tttataagcc gttcttgctt
    20401 tgctttttta tcgaccgctg cttcaaactg tggatttgcg gtgtaaaaac caaactgcca
    20461 gtgaggaaag gcctcaaaaa gttttggaat atctgtgtat aatgctgcca cttcgtcttt
    20521 attcatcagt cgctcaccaa aggggaggtt tgacaataaa ataccctcgt cgtacggcgc
    20581 ttttaagtcg cgaaagtctg ctatttcaaa gtcaggtctt tgaatgcgcg catcactgcc
    20641 aatgagttgt aacgctcttc ctgcggttac gcatgcatgt tctgcatgga gtcgtgcccg
    20701 ttcaattgct tcagcgtcta tgtctgtgcc ggttatacgc gcatgtgcat cggggcgtat
    20761 ttttgcagct tcttttttga gtatttcatc tacacgcttt tgatcgtaga aggagagatt
    20821 ttctaaggca aaagagcgcc caaaacctgg tgcaatattg tatgcgtaaa gagccgcttc
    20881 gacagggata gttcccgaac cacaaaaggc atcgtgaagt ggcatttttc tcttccacat
    20941 catcgcctgc aaaaggcttg ctgctatcgt ttctcgaagc ggagcaggac cacccttatt
    21001 tctgtaacct cttttatgca agggctctcc cgaaagatcg agtaaaacaa gaacctcatc
    21061 tttttctaca tagatacgta tagttgaaat actacctgtt tctggcatag aagccatctt
    21121 ccataccgca ccaagcttgt catagatagc cttgtggctc atgctttgta ttgtatgctc
    21181 gctagaaaga agactttgtt ttgtacgcac cttatcgata atgaggcgaa catcttttct
    21241 gaaaaaatcc tgccagttaa ttgcatatat gccctcataa agcgcatcaa aatcttttgc
    21301 ggtaaaggct gccatttgta aataaattct gtctgcaatg cgtgagcaaa gatttgcccg
    21361 aaaaagtgca tcgtcatcac caataaaggc aacacgccca gattttgaat agagcggttt
    21421 gtaaccgagt tgttttatct catttgagag aaatttttca gcaccgagtg ccgatagcgc
    21481 aataagtgta tgcatagcga cagaataaca ctttttgacc aaatgtacaa ggtgaaattt
    21541 ttacagcttc gcgttcttgt ccaagtctta aaaacttgct aaaataaagc gtatattaca
    21601 aaacaatagg ggttttcatg tctgttcgtg ttagatacgc tccctctcca acgggaatgc
    21661 aacatatagg tggagtgcgt actgcgcttt ttaattatct ttttgcacgc tctcaaggcg
    21721 gctcttttat ccttcgcctc gaagatacgg accgtacacg ctacgatgaa gcctatgtgc
    21781 aaaatttata tgacactctc aactggcttg gcattgaatg ggatgagggt ggtgaaaagg
    21841 ctggggacta cgggccctat gtgcagtctg agcgttttga tttatataaa aaagcggcag
    21901 atgaattgat agcaaagggc gaagcctatc attgtttttg taacgaagag cgactttctg
    21961 ccctaagaac cgaacaaatc gaaaaaaaat cttctgttct cggttatgat aggcattgta
    22021 gagaactttc agatgatgag gttaaaaaac gccttgcttc tggtgaaaaa tctgttattc
    22081 gtttaaaaat tccgcttaca ggggagaccc attttcaaga tgcgctttta ggtgatatta
    22141 gctggaaaaa tagcgatatg agtccggacc cagtgctgct taaaaccgat gggtttccaa
    22201 cctatcatct tgcaaatgtg gtcgatgacc attatatgca gataagtcat gtaatgcgcg
    22261 cacaagaatg gattccttca acgcccttac atgtgagaat gtaccattct tttggctggg
    22321 aagctcctgt tttttgccat ttgcccatgg taaatggaag tgatgggcaa aaactttcaa
    22381 agcgtcatgg ggcaactaat atgaacgagt ttcgctctcg tggctatctt cctcaggcta
    22441 ttataaacta tgttgcgatg ctcggctgtt cttacgaaga agggcgggac cttttccccc
    22501 tcgaagattt agagcgcctt tttaagatag agcacttaaa caaggcaggg gctgtttttg
    22561 actacaaaaa actcgagtgg tttaacggcc aatatatcag acttttaagc gatgaagcgc
    22621 ttttggaaaa aacacttccc tttatcactg gtacggggga tgccctgctt cccatacatg
    22681 caagcgatgc ctttcctgct cctcatacgg gaccagaata ctcaggtatt tccatgaaag
    22741 aggacggaag tcttattgct gccaatatga acgatgcaga aattcgggct aagcttttga
    22801 gtcttatgcc gcttatcaaa gaaaggcttt catatctaac tgaagcggct gaaaaggttg
    22861 cctttatttt taagccctta ccagaaattt caaaagaagc cttgattccc aaaaaactcg
    22921 atgagtgcaa aacgaaggaa gtgctttctg gtgcaagaga ctttatacga gagctcttcc
    22981 tccttgacga agcggagcaa gagcaacttc ccaaaagcta tgcagaaaaa ctcggggtaa
    23041 aactcgggga ttttatgatg cctttgcgta ttgcgctcac cgcaagcaag gtaagtccac
    23101 cacttgttgg ttcgattatc gccttgggag agaaagaagc tttgctacga ctggaaaaaa
    23161 gcatagcctt gctttcttgc tagtatattg ctatactgtg ctaccataaa aaaagagatt
    23221 tagctctgtt tttttatttc catctgggaa atattttttt attgaaacaa agcgtaaaaa
    23281 tgaggagtaa ttgatgagcg aacgaagcct tacaatggaa aaaatagtga gtttatctaa
    23341 aagacgtggt tttgtctttc aatcaagtga gatatatggc ggtcagacag gagcttggga
    23401 ctatggtccc ctcggcattg aactaaaaaa caatattcag cgagcctggt ggaaagaaat
    23461 gacccaaatg caggacaata ttgtcgggct tgatgctgct attttaatgc acccaaaggt
    23521 atgggaagct tctggtcacg ttgcaagttt ttccgaccca ctcgttgact gcaaagactg
    23581 taaaagtagg tttcgtgctg acgatgacac acaaatttct ctcgaaaatg ttgaaaaaag
    23641 aaaatgcccc aaatgtggcg gaccgcttac ggagcctcgt gcttttaacc ttatgtttaa
    23701 gacatcgata ggtgcggcag aagactcatc ttctacgatt tatctacgtc cagagacagc
    23761 tcaaggtatt tacgttaact ataaaaatgt tgcccagtca aatcgcttga aaattccctt
    23821 tggaatagca caagttggta aggcttttag aaatgagatt gtaacaaaga actttatttt
    23881 tagaacctgt gagtttgaac aacttgaaat gcagtttttt gtaaaaccag gggatgatga
    23941 aaaattcttt gcgttttgga aagaagagag aatgaattat tatccaaagc ttggtattcg
    24001 aagggaaaaa cttcgctggc atgagcatga aaaacttgca cactatgcaa aggcagcctt
    24061 tgatatcgaa tacgaattcc ccatgggttt tcaagaactt gagggcatcc acaaccgaac
    24121 cgactttgac cttacacagc atatgcagta ttctggcaag gacttacaat atatagacca
    24181 agacaatgga aacgagcgtt atattccata catcgtagaa acaagtgctg gtttaacacg
    24241 aaatgttctc atgttccttt gcgatgccta tgaagaagaa aaggttgcag acaatggcaa
    24301 tgcagatgat tggcgtgtcg tgcttcattt tcacccaagc attgcgccca taaaagttgc
    24361 tgttcttccc ttaatgaaaa aagatggtct tgccgagctt gcaaaagata ttcaaaacga
    24421 gttaaaagaa gagtttgtaa cggactacga ccaatcaggt gcaatcggta aacgatatcg
    24481 acgacaagat gaaataggta ctcctttctg tgttacaatc gattacgagt caaaggaaga
    24541 tgattctgtg actcttcgtt atagagatac aatggagcaa gttcgcgtaa aacgttctga
    24601 tttagttgag tttatacgaa aagaaatgaa atcctacaag cgtccctaaa ccttgctttt
    24661 ccagtggagg taaaaatgga atacgttgcc ctaggtaaaa caaatctaat ggtaagccgc
    24721 acttcttttg gtacgggggg agtcgaagat ctcgatgatg aggaagcgct atcgattata
    24781 aataagggct atgaaaaagg tatcaatttt tttgacctag cctctgtttc acttcaaagt
    24841 cctctacgtt atacctttat cgataagcgt gattctgtta ttttgggtgt acaaacaagc
    24901 gcaaaaaatg caaacgagct cttggaagaa gttgaaaact tttttaccgt atttaagaca
    24961 gattatatcg atttattgca tttacatcat ttagcctttg ttccagtaaa aaacgatagc
    25021 tctggtctca ttgcttgttt agaaagactg aaagaagagg gcaagataaa gtattatggg
    25081 ttttcaacag aatctttgag cttggcagaa gaggctatag aatctggctt gtatgcgaca
    25141 ttacgctttc cttttaatta tgtctcgaat gcgcaagaaa tagatttgct tagactttgc
    25201 aaagaagcag agcttggttt tatagcctac aaacccttac tcggtggaaa aatacaaaat
    25261 ataccgcttg cctttggttt tttacgacaa tatgaaacgg cagtgcctgt ttggggtttt
    25321 cgctctgagg atgaattaca gcaaattata tattttgaag agcatccacc tcaggtcgat
    25381 tcacaatttt tacaagacat agaacacgag aaagcacttt tttttggtgt ataaaaaata
    25441 aatactgtgc ttagtctgat agcttagtta gtctaaggct aggagctttt ttgctttacc
    25501 atttccattg ctttttgctt tgttaaaacc tgtgcaattt ttaaaccaaa ggtaccagtg
    25561 tttaaagttt taggttctcc ggagggctca aactcgttga gtatttgcac aatgggattt
    25621 tgcgggtttt ctgggtttac atctgttacc tgtgctactt ttccattgga tagtaaaacg
    25681 tagccaccaa taggatacag tgacaatgat aataaaagcg cttttataac tgtcggatcg
    25741 tagacctgtc cgttgttttt gagcatttct atcattgcct catgtgaaga agaggcttcc
    25801 ttataatgtc gtggtgcggt aattgcttca aaagcacagg ctacagcaat tatcttcgca
    25861 taggttgaaa tcttttcgcc agaaagttta cgaggatagc cggtcccatt ttctctttcg
    25921 tggtgctcaa gtacaccgag ggaaatattg agtggaaatt cattttcttt taagatattg
    25981 tatgaaataa tgggatgggt aaaaatagta tttttttcat tttgtgaaag cggtctgtcg
    26041 gtcatgtaaa gctgtggagg caaacgtatc atacctattt catgtaagat acaagcaaca
    26101 ccgagctcta taagctttga aagaggaagc cgcatttgca aaccgatggt tatagcaagt
    26161 actgtcgagc gcatcgagtg gctaatgagg aagtttttat tctttgcttc tagacttggt
    26221 tgtattctaa gaacaaatct cctattttct tttacaaaga taacaaattc ttttacctta
    26281 tcagatagct catctaattt cagttcttta tgtgtcgcat agcgtgtgta tacttgattt
    26341 atataattaa aataatcgtt ataaatagtt tgcacaacgg aaagcctgct tttatcggaa
    26401 tcgcctttta tttctcccgt aaataaactg gtatcaacct cagttgcatt ttttaaaata
    26461 tctgggtcta tgtattcctt ttttttagtt tcactgtcag ttggctcctg ggggctcgtt
    26521 ggcgtaaaag aatccatgcg agctgttcca ctaccaatag tcccatcaga atatacttgc
    26581 ttaaaatccc atattaaaag tgcttttata agtgcgtttg aaacaggaat agagtttgtg
    26641 agaagtaaaa atgttttatc tataactaca tcttgagtaa aataaacatt ttcttttaaa
    26701 tcaactacgt taaacgaatt cactatctgc tcctcatagg cagcgtttca aatttttttt
    26761 ggttgctacc ttgcattttt acctgtttct atctactaat ataacatgtt ttttaaatag
    26821 tgcaaagttc tttccataag tcttataaaa aaggaatggt cgaaaaaaaa gctgaaaaac
    26881 tcaacaaaga aaaagttttc cagctttata agtacaagac ttagccgtct ggatgtcgaa
    26941 ccggctaaat gattgttctt tatgatactt atagtttcgg cagtttatat aaaagcttaa
    27001 gcgtattgta aagatttttc caaaaaaaga attgcctttt gggcattttc aaaaaagcaa
    27061 aacaatacag cgtgagaaaa ttctttgttt tccagcattg cttttt
//
LOCUS       DAOSSW010000012        26817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_2001_length_26817_cov_6.723675, whole genome shotgun sequence.
ACCESSION   DAOSSW010000012 DAOSSW010000000
VERSION     DAOSSW010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 26817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26817
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2001_length_26817_cov_6.723675"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(410..1462)
                     /locus_tag="PKW26_02125"
     CDS             complement(410..1462)
                     /locus_tag="PKW26_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92399.1"
                     /translation="MKKWLGSNFYLPSLMARGLNCVDYFILDWLYVFKESGKMHSKKV
                     FFSKPRQYLTGVYVKYQKIIDDLQKMIPIQTTETIGKRIQKYEDAGLIYKALVKNNGT
                     FVYIHFIEEQWNSLVSEIKSTEKTEYPDLNPGIDGSKAVSRLVFKTGTNNSKAIHSKV
                     TNTGDVKETKSYAIVAKVFGEKVSEFSSDFFMNLDDFFEKHFLEKEKRQDYLSYIKAY
                     ALEKIKTEDALAGYVYKTVFLENMLHSFLNLLNQKPKVETSSKSNLHRCKACGEDYEE
                     SEPYCPVCQLTISEESDKEKVEYHHRVSLLSPEKRTAFFLALSATLLRNWKKGKSSAS
                     EDTLNEIKKEYGLIDN"
     gene            complement(1440..1646)
                     /locus_tag="PKW26_02130"
     CDS             complement(1440..1646)
                     /locus_tag="PKW26_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92400.1"
                     /translation="MTNEVLLEIKTEIEKQNITHGKCTVCFTFHDGRLGFYELQFSKR
                     KNIQPNKQLKIKTGGDGFEKMARK"
     gene            complement(1643..1864)
                     /locus_tag="PKW26_02135"
     CDS             complement(1643..1864)
                     /locus_tag="PKW26_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ92401.1"
                     /translation="MNTSKITSIKNLTEETLLSRQEVAKILHIGLSTLDSLITEKELP
                     RTRIKKRVFVLQQDLENYILANRNTGGAK"
     gene            complement(2029..3288)
                     /locus_tag="PKW26_02140"
     CDS             complement(2029..3288)
                     /locus_tag="PKW26_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92402.1"
                     /translation="MGARSFKWKNLEAKIDERVDRYALLLAIANIQCSVYSLEKYNGE
                     QKKAEKFFLKNILIEAEKSQNNTGNRFYNSDLFNKAVNFQGEKKDAEKIAENLYKKIK
                     GKVIFDNEKYSLIQNLGALISLFLGILFFCITLVCIVYSRAALDYFFVSLFFFSISGA
                     FFYKYRIVNGLSKNSVKTFYDRILVEDVFYFLEEEERYFKEVKAKREQNEKEEIQRVR
                     DQKDKKEKEEFKREIEELKKARDQNENEKNKTGIKYDFFDFQDEYFYLPFPFLIALSR
                     VESKKRKALDDNYIYNKYFSLHLLLASIDYYKCKYFNGEKEKEKEEEEEKEEEEEEEE
                     YSWSILKKVLGMDKKIWKKNFANILSQAKKREGKKTKSALEQEEYIQLIDAEVKKIDE
                     EHRERTEKRFLPKDMQALFFAKRIPKE"
     gene            3592..3681
                     /locus_tag="PKW26_02145"
     tRNA            3592..3681
                     /locus_tag="PKW26_02145"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            3759..3839
                     /locus_tag="PKW26_02150"
     tRNA            3759..3839
                     /locus_tag="PKW26_02150"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3793..3795,aa:Leu,seq:tag)
     gene            3868..3939
                     /locus_tag="PKW26_02155"
     tRNA            3868..3939
                     /locus_tag="PKW26_02155"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3900..3902,aa:Gly,seq:gcc)
     gene            4026..5399
                     /gene="tig"
                     /locus_tag="PKW26_02160"
     CDS             4026..5399
                     /gene="tig"
                     /locus_tag="PKW26_02160"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOQ92403.1"
                     /translation="MKSSHTVSKKEASQASLAVTVKAADAEGKYTETLQKYAKSIQIP
                     GFRKGKAPLSVLESKYGESLKAEAAAEIIDACLEDIFTKFDEEKSEYRPLAYAQPQMD
                     SVPELKIGEDFSFEIRYDIMPSVKVTDFSGITIKEPQVSVGEAELKEELKAIQERNAM
                     VVDKKDDAKAKKDDIVTINYCELDEKGNALEDTKREGFVFTIGSGQNIYKIDDEIIGM
                     KKGETKDITKTYDDKEEDPDLKGKTKHIRVTIDGLKERNLPELDDELAQDVHEKYKTL
                     EDLKTDIRKNLDTALENRLRELKSNDLIEQLIEKYPIELPQSMIAAELESRWRMMAQQ
                     FQTTVEQLDKMVASSGQTKEAMLNQMSGDSEKMLKGRIIVESLLKDSDISISAEEVDA
                     EFEKIAEGAGISLDEVKKHYADPHRKEYLIDDMKEQKLYKDLFTKVKISKGDKLTFAA
                     LFENTAE"
     gene            5435..6046
                     /gene="clpP"
                     /locus_tag="PKW26_02165"
     CDS             5435..6046
                     /gene="clpP"
                     /locus_tag="PKW26_02165"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOQ92404.1"
                     /translation="MNEKMHTLIPYVIEQTSNGERSYDIFSRLLQDRIIFVDGEINDI
                     SADIIVAQILFLESQNAEKDISMYINSPGGSVTAGLAIYDTMQHVRPDIQTICMGQAA
                     SMGALLLAGGSKGKRYALPSSRVMIHQPWGGAQGQAADIAIQAREIIRLKKLTIEYFA
                     YHTEKPIEQIAQDMERDLFMSSEEAMDYGIVDHIMKRREDGSK"
     gene            6033..7289
                     /gene="clpX"
                     /locus_tag="PKW26_02170"
     CDS             6033..7289
                     /gene="clpX"
                     /locus_tag="PKW26_02170"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HOQ92405.1"
                     /translation="MALNKDRKTCSFCKKPEGPNRMLIKGADQSYICQQCSSICNSIF
                     AQTDSDESEFILTDIPTPKAFKEHLDQHVVGQDYAKKVLSVAVYNHYKRLSLQGSRFE
                     KDADEVQIEKSNILLIGPTGSGKTLLAKTLAQKLQVPFAIADATTLTEAGYVGEDVEN
                     VLLKLIQAAGGDIAKAEKGIIFIDEIDKITRKSENASITRDVSGEGVQQALLKIIEGT
                     IASVPPQGGRKHPNQEMIPMNTENILFICGGAFVGLEKIIEKRVHDHSMGFGAQTKLR
                     SEESMMELLNKVIPDDIVKFGLIPELVGRLPLSVALKELTEDDLVTILTEPKNSIIKQ
                     FKASFLFDNVDLQFTDEAIRAIAHTAIKHKTGARGLRSIVEKMLLDIMFEVPSLKEGK
                     SLTITEDVVLNTEKATIQLLKKNKAS"
     gene            complement(7286..8398)
                     /locus_tag="PKW26_02175"
     CDS             complement(7286..8398)
                     /locus_tag="PKW26_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOQ92406.1"
                     /translation="MKIGINTFGCEHGRSGIGSYLISLFKNIDSKTKHNFILFGEEID
                     RYTYSEAFANSFEAVPISTKKPVLKLWHRIGILSFIKKQKFDIVLYPNPISFMPARFS
                     VPSVALVQEIASLALAEKPFFKRAFIVRKLKKVHKIIAGSQYIRKDLIQLGIKDAKIE
                     VIHNGIDHSLFYPHRELTGDTLVIKPFSIKRPYFIYVSRLQGEHKMHRELIDAFDLFK
                     SKTKSEHRLVLSGSDGDKAERIHKAVAASAYAEDIFLTGHFPHENLPELYSCADASVF
                     PSIAEGVGLPILEAMATGIPVLCAKAGSLPEFAGNSAIYFDAHNPVEIAENLERIVND
                     EKLRETMIENGLDWVKRFSWTKTADKTIEVLENLEA"
     gene            8446..9588
                     /locus_tag="PKW26_02180"
     CDS             8446..9588
                     /locus_tag="PKW26_02180"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOQ92407.1"
                     /translation="MENDNQSFSFFSLQKQFIDNLDKQGIVKPTAVQEKVIPAILEGK
                     NVFFESETGTGKTFAYLLPLLEKVSFTNKDVQVIIVSPTHELASQIKGQIQILGDFLV
                     NLCIGGTSLKRQIEFLKEKPQVVIGTPGRILELVHLKKLKLAGLQAIVFDEIDRLMAK
                     ELRDDTMLLASLTPSESQLIACSATMKENLSEKIALSAKKEIESIYIEPEDILQKNIE
                     HIALFSEQRNKVDVLRSFLAAVKPKKTLVFTSKLSDVQIIASKLAARKIASASLFASA
                     SMKERKAAIDGFRSGKFQVLVTSDLSSRGLDIQDVSHVVQMDYPSNEVFFIHRAGRTG
                     RSGNMGYNVLIGDAWEMREFSKLEKKLGIKITPKQLSSGKLLAPKL"
     gene            9663..10628
                     /gene="hflK"
                     /locus_tag="PKW26_02185"
     CDS             9663..10628
                     /gene="hflK"
                     /locus_tag="PKW26_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOQ92408.1"
                     /translation="MNEEKETSKKKQMKFSPGTFILGIIVLFALILVFTSFFIVDATE
                     QAVITRFGRYHKTLGSGLQFKLPLGIDKSYNIPVKVVQTEQFGYKTIKGGITNKYSNE
                     MFKESTMLTGDLNIVDVEWIIQYRIVDPSAWLFNVKDKQQTIRDISQSVINTLVGDRA
                     ILDVMTTERSSIQDNAVEMMNDNFKSLGLGINIITVQLQNVVPPADVQAAFEDVNKAS
                     QDMERFINEGLERYNAEIPLAKGVADQQIQIAEGYAINKVNRAQGDVSRFLAVYDEYR
                     KAPAVTRDRLYLEAMEQIFKAKQNTTLVDKELQNILPIKNLGGAQ"
     gene            10625..11608
                     /gene="hflC"
                     /locus_tag="PKW26_02190"
     CDS             10625..11608
                     /gene="hflC"
                     /locus_tag="PKW26_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOQ92409.1"
                     /translation="MKKFTIGLIIVIAVFIVFLMMGPFYIINEGNQVVVTRFGEIVET
                     HTEAGLYFKIPFIDIVTTYPKLILSIDGDSQRIPTKENQFIIVDTTSRWKISDPALFY
                     QSFKTLEAAYTRLSDVIDSATRTIITQNRLSEIVRSSNLINEEESIDPSIESLSDEAT
                     DQISSLINVATKSESVHKGRRHLTNEMAIEAQKMLPQYGIELIDILPRQIKYSDEMTE
                     SVYNRMIKERNKVAQAYRSFGEGQKADQLGKLEKDKKRIESEAFRTAQEIKGKADAEA
                     SKIYADAFSVDPEFYTFWKSLESYQETIPLFDASFSTSMDYFKYLYNARGR"
     gene            11609..13156
                     /locus_tag="PKW26_02195"
     CDS             11609..13156
                     /locus_tag="PKW26_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92410.1"
                     /translation="MNLKAHEDAVYLHTEIGSREFAQSKLFPLLKEAGFLVSFNDSEI
                     DFTEWFFTGTQEDAGKKTKIMTLIGPGFEAKSFYEILMEGEREKKQDSIYRIQRAYTE
                     ALEKNIALPNTGPMGILVSEDSLLFLPQELCLRSFNALSETKKSEFFGRFKNRSLKGQ
                     ESIDFCLAVYIYIYLTGDFPYPSLDEEKRQEHIQYAECIPLHLYNPSFPQDMIFSVES
                     ILQGKKEKPSLPLLDKSYLEPEITEKNLDILREKERKEWLEKKQKRNSRIIFLKKHAT
                     KLIIFPAIFFLLALTIIGFMRDKKAGPNSLGLSDIETIEAYYTAVNTLDLSLSSNLLY
                     KKTKSPYESIIATYHVVKMTRESYERIRPFVHPIEKIQNASLVDSGMFGISHLKIGDT
                     LLNPFSQKASAQNKIAVPDIADNEKRQYTVKFYFIKNEGEDDLVVEFCEDYVELVFHK
                     DRWLITKVLPSSNIQIEDYVLFLEKLENIAELPLEEKIKLLEKEYVWMPSLEELYFIQ
                     SEREDDE"
     gene            13149..13640
                     /locus_tag="PKW26_02200"
     CDS             13149..13640
                     /locus_tag="PKW26_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOQ92411.1"
                     /translation="MSEKAYKRPSWDEYFMEICQTVAKRATCDRGRSGCVIAKENQIL
                     VTGYVGSPTGLPHCDDVGHKLKKMVHESGEVTQHCVRTVHAEQNAITQAAKRGIAIDG
                     ATLYCKMTPCRTCAMLIINCGIKRVVCEKKYHDSKDSLEMFSQAGVEILHLQEEVQKY
                     ENM"
     gene            complement(13664..14257)
                     /locus_tag="PKW26_02205"
     CDS             complement(13664..14257)
                     /locus_tag="PKW26_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOQ92412.1"
                     /translation="METPKDLTKYCNKCGSFLVSEKETCLSCRDSPLLEHVDFVLPLH
                     SYRLWKKEVLFYWKILGNRSFSPVIAKMYAEVLERFFFGIALVPIPPRPGKIRKTGWD
                     QIDEITKILSLNKKHKILNLLKRVSRRQQKKMNRQERLDTIGRVYALKDGIKDLPSKV
                     VLIDDIMTTGATLESCALVLKEAGVKEVYALCLFIGS"
     gene            complement(14422..>15713)
                     /locus_tag="PKW26_02210"
     CDS             complement(14422..>15713)
                     /locus_tag="PKW26_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92413.1"
                     /translation="DTSDINNSTDTKPIPDELVGEDFETIEEKNDILFEEPIVESLGF
                     FPEQDLESDENQAVFDNVVSDEVADSDKSEKKLIPVQTQEVKTEAKIWQGDLETEKTD
                     IVPSTEEVITEIEGLQRTPIQVPSPPASPQVAELAQAPQITQAPVVQTPSPSPPIQEA
                     TKAQEIKTENIQEAQFAEPEEKSLPEIKELASKTKADAKNNQFLEIRYPGSGWIYIGE
                     EGQENFVSYVGRKIVDGDTLFTLKTTKPGLTRLEFYKSDPLSQSYIEDTIELTISDER
                     ALDSEKKLIDSYDYEDALKAPEAITAENEGQKLLAGSQSALSDEPELEELAFLPPVET
                     MPSEMLLSSAKESMANNAPEDALLYLDEFLRVSLDNTDEAWFLRGQLYEKPGKMRNIQ
                     LARESYQTIVDAYPSSAFWKQASDRILYIDKFFFIIK"
     assembly_gap    15714..15813
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15878..16300)
                     /locus_tag="PKW26_02215"
     CDS             complement(15878..16300)
                     /locus_tag="PKW26_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92414.1"
                     /translation="MIDRVKQVFSAFQDFIANLTYSILHKTGLDVFYKKYPKRFFTVL
                     SIILVALFLSVTVYGKVFIKKAVIPQREIYNFDLDQDLLPPAEPLFREEYYLSREQKE
                     KWSDEDIQEWFTIPNEYMIEKLHDDNTRLIQGIMEGSP"
     gene            complement(16332..19235)
                     /locus_tag="PKW26_02220"
     CDS             complement(16332..19235)
                     /locus_tag="PKW26_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ92415.1"
                     /translation="MFLKSLEMFGFKSFADRTKIDFATGITALLGPNGCGKSNVVDAV
                     KWVLGEQAARSLRAEKMEDVIFNGTETRKALNFAEVALTLANDNGLLPLDTSEIQLKR
                     RLYRSGESEYFINNTQVKLKDVRELFWDTGVGKTAYSVMEQGKIDQILSSKPEERRFL
                     FEEAAGITRFKAKRAEAERKLERTEENMRQVDGILGEVKRSHDSLKIQSDKTHQYRAL
                     RESIFNFELDIQLLRLKGFINDKGRKEEDLKTASEKRDIARKEIDEINASVAEHMDAV
                     NEMQTKLIAMNQEIFGLAAEQKGKQDQIKIVTEQHAEAKAKIAVLENRKKNLEEQIEN
                     LQEEIDEKEASLHQIHKRIDDIVNNISEFESNIKLASNQIYKNSDDVKKLEGDIDSLE
                     KENRGFQTELEAITEDIVSELDQKLKDAGYSSKAHEEALEKFTALMANIKRLASSKKT
                     ISQDFSAIQEHTESDISQFSQMVLSSFSELEDSLKDLENAFDEYKKTSLHFIDDFLSP
                     EGIITKKRRIDEKIKQTLQKMQEKKQEIVKLEEENKVLSQKINEYRETLEKLRINQAQ
                     MRSEVQASEDQIRIIRRELRNQENALSDLENEVFTEKKRSEEINEQIIELEGEIASIE
                     HRGRTLTKELENLEYSISSKNKDVSGKQDAVQKKTQELGKYQAQLEKINLDIVTIDTE
                     IRNLKDNFRESHSRDLLEFEEKMYTITMPIMSLRESLSKARQELKDIGSVNLMAPEEF
                     LEVKERYDFLTKQIADLVKARDDLKRITEEIRAESTELFMATYNNIKKNFHNMFRRLF
                     GGGKAELRLTDPQNVLESGVEIFAQPPGKKLENIALLSGGEKTMTAIGLLFATYMVRP
                     SPFCLLDEIDAALDEQNVLRFVHTLREFANVSQYIVITHNKKTVTGAGTMLGVTMEES
                     GVSKVITIRLENEGANSDSSIPYIEPFEEEDVPLEEDVYMPPHPPKRVRNEQR"
     gene            19362..20636
                     /gene="serS"
                     /locus_tag="PKW26_02225"
     CDS             19362..20636
                     /gene="serS"
                     /locus_tag="PKW26_02225"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOQ92416.1"
                     /translation="MLDYRFIKENIDAVKQNIKNRNMTSDADLVVELFNKRTQLISAL
                     QEFQQKRNENATAMKQKLDEAQRKALIEEGKALKEKISLMEAELKEAEEALDREGKKI
                     PNMAHPDAPIGSLDTENLEVKQVGKPREFSFVPKDHVELGQNLDLIDFEAATKVSGPK
                     FYYLKNEAVFLEQALTMYGLQILKKHGFTPFITPDIAREEILTGIGFNPRGNESNVYS
                     LEGEDACLVATAEITLGGYHKDEILAKERLPLRYCGLSHCFRREAGAAGQFSKGLYRV
                     HQFTKLEMFVYCTPEDSDAIHEELRLIEEEIFTGLGIPFRVVDTCTGDLGAPAYRKWD
                     LEAWMPGRNGGEWGEVTSTSNCTDFQARRLNVRYKNEDGKNTFVHMLNGTAIAMSRAL
                     VAIFENYQNEDGSIDIPEVLQGLCGFSKIEAK"
     gene            complement(20701..21648)
                     /locus_tag="PKW26_02230"
     CDS             complement(20701..21648)
                     /locus_tag="PKW26_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92417.1"
                     /translation="MNNYLSKVERDLILSHLIDEKPLLLFKPLSTEYLPHEAEAYQFP
                     QITIAPQSYQVVNKGILFFPFSDFSFSIPHGTSIAVLFHFKGRALIFESQFSHLTKGY
                     ALVISPYIFKQIDDSISQEKRIHGKFYFGLQNESTLFVPFHSKASIPLFSIDLWKDLS
                     SESEKKFLSLVESLYQKTYSLSELFIEKKAADTKPFYPLISAAIYLTQKEERGAESFA
                     GRARPLEILFLSDTHIVFASESIREIMHEKSEYFITIEIPVLRLTRHIDSTIFVEQIL
                     FDSVEGQKKEIAFCHISSLQEENRRFLSEKLYGFDSEFD"
     gene            complement(21641..22300)
                     /locus_tag="PKW26_02235"
     CDS             complement(21641..22300)
                     /locus_tag="PKW26_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92418.1"
                     /translation="MKLSYSALFCLFSLFLCSCKIDKNLYQYEDEVLSLKNEFNQKTR
                     DIPWIEEIEADILYTTEADKKSSAKKTDTSDTLLTSDMLFLHDSNTSRAIYPYIEGFG
                     SLDSRSLDPKTRYFLDAFLKELASARYAKEAFLPDARYLSIILTDFIENYPLPVSWIF
                     GEAFPLSSPTANYLEVPVKIYSDKTQDNKKAHYKLVFFLVESEEKKRIQQIQIGNIIY
                     E"
     gene            22398..24380
                     /locus_tag="PKW26_02240"
     CDS             22398..24380
                     /locus_tag="PKW26_02240"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOQ92419.1"
                     /translation="MALNLSQKILAAHRVSKDGNDIAIKIDQTLTQDATGTMAYLQFE
                     TIGIPRVKTDVSVSYIDHNTLQTDYRNMDDHRYLQSIAEKYGLWFSRSGNGICHQVHL
                     ERFGKPGATLLGSDSHTPTGGGLGMIAIGAGGLDVAVAMGGGAFHLTEPKIYGVKLTG
                     VLKAGVSAKDIILEVLRRETVKGGVGAIYEYFGPAIAQLTVPQRATITNMGAELGATC
                     SIFPSDEVTKNYLAAMGREKDWIALSADEGASYDKLIEIDLSSLEALASQPHSPDAVT
                     EVKKLKGKAVQQVVIGSCTNSSLDDLSRVASIVKGKHIHPGVEAGIAPGSRTTLLLAE
                     KMGILGDLIKAGFRILESACGPCIGMGFAPSSGGVSLRTFNRNFKGRSGTPDALVYLV
                     SPETAAAAAITGEITDPTELSVSYINQEKSLLDGDNPLLSSEDIAAISSDDGMLIAPK
                     TPEEAAKVEIIRGPNIKPCPLSPQLKASLTLPVLLKAEDNVSTDDIMPAGAKVLPLRS
                     NIPEISKFTFERLDECFYSRAIENQKVNPNGCIVVGGENYGQGSSREHAALAPMFLGV
                     RAVLVKSFARIHKANLINYGIIPLVFKDKEDYENIAFGEKLVLEDIFESLDTGKNFVL
                     KTASGKTIEAINDLDERSKAILKAGGLAAYTKSGGQ"
     gene            24393..25631
                     /locus_tag="PKW26_02245"
     CDS             24393..25631
                     /locus_tag="PKW26_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ92420.1"
                     /translation="MQAVRDPLWKHIFLPDALYEATNTKDFLRLHGIKQLGPSYLVYP
                     GATHTRAAHSFGVYEVASRLIKNLIQKGARNWVSREGMQSFLAASLFHDLGHFPYTHS
                     LKELPLLDHEVLTGEIILKEPLRSIIGKTGANPEQVAAIVDTSKDCSDVETLFYRKLL
                     SGVLDPDKLDYLNRDAFYCGVPYGIQDTEFVLAKLIPHKERGIIIDSSAMLSCENVLF
                     SKYLMYKAVYWHKNVRIATAMMKKTLYTALKEGILQAKELYSIDDQGLYSLLESKTYF
                     DELECADAVQNKAFFKVLYECPYNESHKLHRDLEDLEKREKIEKEIAEKIGCKAAQIL
                     IDVPERISFESDLYIRDEKTSFSKSSTVFSEHTVERFTTSLRKIRLAVSPLFLSQKTD
                     KETEKNIKLIEEFFFLDYNT"
     gene            25644..26351
                     /locus_tag="PKW26_02250"
     CDS             25644..26351
                     /locus_tag="PKW26_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HOQ92421.1"
                     /translation="MKVLNYMEDLVITRTNELYDEVKEMDAPWLNCDCEHCRLDTISY
                     ALNRLPPRYIVSGRGFIHNTVDQDSQLKADIDSLVIEGMHTINTTQRPYHGTAKGKMK
                     GQTGPYFNFPTFIGSVLDGTTFEPLSDVEIILKQDNVKSEMVDYTWANPYISSKLTKG
                     TYSFLVKSEEAEKENISKVFVFTLEAKKEGYESITYNFSIPLISEATAKVESKSTYSL
                     KIQNLFMFEEGSTNELE"
     gene            26417..26489
                     /locus_tag="PKW26_02255"
     tRNA            26417..26489
                     /locus_tag="PKW26_02255"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26450..26452,aa:Pro,seq:tgg)
BASE COUNT         8348 a         5083 c         5116 g         8170 t
ORIGIN      
        1 cgatattttg gatatccgca gaagagaaaa caggatgttc aattgtctcg ataagagctc
       61 ttaccaatct attcataatg ctaactgtca cattatgcca gttattcgat attttcgact
      121 gattcctagg ttttaccatc agcaacagct attaagtatc ccttaataac tgaatcgggc
      181 gtataaatca agtacatttt tttgaaacaa ggtactctgt ctttttcatg tttttcacaa
      241 atatgagcct ctactttccc aaagtcatcg ctaaagaggc tatcctcaaa ctgaactata
      301 aaatatttct tactagttgc gcatcctcta atcctcatcg tcaaagctac tactttttat
      361 agcaagacca agggagaatc ccttttaaga ttctcccttg gtctttagct taattatcta
      421 tcaagccata ctctttttta atttcgttta acgtatcttc agaggctgaa ctttttccct
      481 ttttccaatt gcgtaaaagt gttgcgctaa gtgcaagaaa aaaagctgtt cttttttcag
      541 gagataaaag agagactctg tgatgatact caactttttc tttatcactt tcttctgaaa
      601 tagttaattg acaaacagga caataaggtt cactttcttc atagtcttct ccgcaggctt
      661 tacagcgatg aagatttgac tttgaagaag tttccacctt gggcttttga ttcaaaagat
      721 tcaaaaaact atgtagcata ttttcgagaa acacagtttt atacacatag ccagccaatg
      781 catcttcagt tttgattttt tctaaagcat aggcttttat gtatgagaga taatcttgcc
      841 ttttctcttt ttccaaaaag tgcttttcaa aaaaatcatc aaggttcata aaaaaatcag
      901 aggaaaactc agaaactttt tctccaaaaa ctttggcaac aatagcatac gattttgttt
      961 ctttcacatc cccagtatta gtaactttac tatgtatagc tttactatta tttgtccccg
     1021 ttttaaacac gagtctagag accgctttag aaccgtcaat accagggttt aaatcagggt
     1081 actcagtctt ttctgtggac tttatttctg aaacaaggga attccactgc tcttcgataa
     1141 agtgaatata aacaaaggtg ccattatttt tgacaagcgc tttatatata agccccgcat
     1201 cttcatattt ttgtatgcgc ttgcctattg tctctgttgt ttgaatcgga atcatttttt
     1261 gtaagtcgtc aattattttt tgatacttca catagactcc tgttaaatac tgccttggtt
     1321 ttgaaaaaaa gactttcttt gaatgcattt ttcccgattc tttaaaaaca taaagccaat
     1381 ctaatataaa atagtcgaca caatttaagc ctctcgccat tagcgatggc aaataaaaat
     1441 tacttccgag ccattttttc aaatccgtca cctcctgtct ttatttttaa ttgcttgttt
     1501 ggctgaatgt ttttacgttt cgaaaactga agttcataaa accctaatcg tccatcatga
     1561 aatgtaaagc agacagtaca tttcccatgt gtaatattct gcttttcaat ttcggttttt
     1621 atttctagca atacttcatt tgtcattttg cacctcccgt gtttcgattg gcaagaatat
     1681 agttttctaa atcttgctgt aaaacaaaaa ctcttttttt tattcgagtt cgaggaagtt
     1741 ctttttctgt aatcaaagaa tccaatgtag ataaaccaat gtgcaaaatt tttgccactt
     1801 cttggcgaga caataaagtc tcctcagtga ggtttttaat gctagttatt tttgatgtat
     1861 tcatactaac tcctatgaaa gcccaaggcc tgtaggcttt cagtttgtaa taaggaaagg
     1921 ctaagccctt cctgtacgta catataagag tgtagcaaat aatagtggga ttattaaaaa
     1981 cgctacttct tgctattatc gatactatat aatagcaaaa aagcgtacct attctttagg
     2041 aattcttttt gcaaaaaaga gagcttgcat atcctttggt aaaaaacgct tttctgttct
     2101 ttcacggtgt tcttcatcta tcttttttac ctcggcatca attagttgta tatattcttc
     2161 ttgttctaaa gcagatttag tttttttccc ttctctcttt ttagcctgag aaagaatatt
     2221 agcaaagttt tttttccata tttttttatc catgcctaaa acttttttaa ggatactcca
     2281 gctatactct tcttcttctt cttcttcttc tttttcttct tcttcttctt tttctttttc
     2341 tttttcgcca ttgaaatatt tacatttgta gtaatcaatt gaagctagca aaagatgcag
     2401 tgaaaaatat ttattataaa tatagttatc atctaacgcc tttctttttt tagactcaac
     2461 tctgcttaaa gctattaaaa agggaaatgg aaggtaaaaa tactcatctt gaaaatcaaa
     2521 aaaatcatac ttgatgcctg ttttattttt ttcattttcg ttttggtccc ttgctttttt
     2581 taattcttct atttctcttt taaattcttc tttttccttt ttatcttttt ggtcccttac
     2641 tctttgtatt tcttcttttt cattttgttc tcttttcgct tttacttctt taaaatatct
     2701 ttcctcttct tcaaggaaat aaaaaacatc ttctaccaat atcctatcgt aaaaagtttt
     2761 tacagagttt ttgctgagcc cattaacaat acggtactta tagaaaaaag caccacttat
     2821 agaaaagaaa aatagagata caaaaaaata atctaaggct gctctagaat aaacaataca
     2881 aacaagtgtt atacaaaaaa aaagaatccc taagaataaa gaaatcaaag cgcctagatt
     2941 ctgtattaaa ctatattttt cattatcgaa aatcactttt cctttaattt ttttatacag
     3001 gttttccgct attttttcag cgtctttttt ttccccttga aagtttaccg ccttattaaa
     3061 aaggtcagaa ttataaaatc gattccctgt gttgttttga cttttctctg cttcaattaa
     3121 aatatttttt aagaaaaatt tttccgcttt tttctgctct ccattatatt tttctaaaga
     3181 atatacagag cattgtatat tggcaatagc caataacagt gcatatctat ctacacgttc
     3241 atctatttta gcttctaggt ttttccattt aaaagatctc gctcccatag cattttattt
     3301 cctttgtttt ttgtatttat atttatgagt ataggcatat ttcaaaaaaa agaaaaggta
     3361 aaaaaagtag actaattgtt aagtgtgtat ttctctgctc tcattttttg taaatatttt
     3421 tatctgcaat attgtattct tttctccctt ctatgccttt ttacgtcaca tttctatggt
     3481 ggagactgtg caatggaatg tgcaatgatt atcgaaataa aaaaatctga agccagaaaa
     3541 agccagaaaa agccaagaaa agattgacga agggcgtttt tggtgctaaa atgagctaaa
     3601 atttagtgta ctagggaaaa tgcacgggtt tgaagccgct cttaatctgt gggtcacaag
     3661 ttcgatcctt gtatggctca gcaaaaaaca tttgttttta gaaaatgtat tgacattatt
     3721 tttcaaaacg agtaatatca atagtgttcg ctagtaaagc gagagtggtg aaattggcag
     3781 acacgctaga cttaggatct agtgccttag gcgtaagggt tcaagtccct tctctcgcac
     3841 tttattctaa aacaagcgac aatttttgcg ggaatagctc agtggtagag cgccaccttg
     3901 ccaaggtgga tgtcgcgggt ccgactcccg tttcccgctc tacataaaaa gcgattcgga
     3961 gtaatctgaa tcgctttttt tatactactt aaaaccatca aattcaagat gaaaaggaaa
     4021 agtctatgaa atcaagccac acagtaagta aaaaagaagc ctcacaagca agtctcgctg
     4081 ttaccgtcaa ggcagctgac gctgagggaa agtacacaga aacgttgcaa aaatatgcaa
     4141 aaagcattca aatccctggt tttagaaagg gtaaggcacc gctttctgtt ttagaaagta
     4201 agtatggaga atctctcaag gcagaagctg cagcagaaat tattgatgcg tgtttagaag
     4261 atatttttac aaaattcgac gaggaaaaat ctgaataccg tccactggca tacgcacaac
     4321 cacaaatgga tagcgtaccg gagttaaaaa taggtgaaga tttttctttt gagattcgct
     4381 acgacataat gccaagcgta aaagtaacag acttttctgg catcacgata aaagagccac
     4441 aggtgagcgt aggagaagct gagctaaaag aagaattaaa ggctattcaa gagcgcaatg
     4501 caatggttgt cgataaaaaa gatgacgcaa aggcaaaaaa agatgacatt gttactatta
     4561 actactgcga acttgatgaa aaaggcaatg ctctggaaga tacaaaacgt gagggctttg
     4621 tttttaccat tggctcaggt caaaatatct acaaaataga tgatgaaatt attggtatga
     4681 aaaaaggtga aaccaaggac attaccaaaa cttatgatga caaggaagaa gatccagatc
     4741 tcaagggaaa aacaaaacat attcgtgtaa caatcgacgg tttaaaggag cgaaatcttc
     4801 ctgaacttga cgatgaactt gcacaagatg tgcatgaaaa atacaaaacg ctcgaagact
     4861 taaaaactga tattcgaaaa aaccttgaca ccgctttgga aaataggctt cgcgagctta
     4921 agtcaaatga tttaatcgaa caattgatag aaaaatatcc tatagaacta ccacaatcga
     4981 tgattgctgc cgaacttgaa tcacgctgga gaatgatggc acagcaattc caaacaacgg
     5041 ttgaacaact agacaagatg gttgcctctt ctggtcaaac aaaggaagct atgctcaatc
     5101 aaatgtctgg cgactcagaa aaaatgctca agggtagaat tattgtagaa tctctcctaa
     5161 aagactcaga tatttctatc agcgctgaag aagttgatgc agaatttgaa aaaattgcag
     5221 aaggtgcagg tatttctctc gatgaagtga aaaaacacta tgcggatccc catagaaaag
     5281 agtatcttat agatgatatg aaagagcaaa agctctacaa agatttattt accaaggtaa
     5341 aaataagcaa aggcgataaa ctaacatttg ccgccttgtt tgaaaatacc gcagagtaat
     5401 ctcgctattt gaattatatt ttgaggatgc aataatgaat gaaaagatgc acacacttat
     5461 tccatacgta attgagcaaa caagcaatgg tgagcgctca tacgatattt tttcacggct
     5521 tttacaagat aggattatct ttgtagatgg tgagataaac gatatttcag ctgacatcat
     5581 tgttgcgcag attctctttc tcgagtcgca aaatgcagaa aaagatattt caatgtatat
     5641 caattctcct ggtggctctg ttactgccgg cttagctatt tacgacacca tgcagcatgt
     5701 tcgccctgac attcaaacga tttgtatggg acaggcagcg agcatgggag ccctccttct
     5761 tgcaggtgga agcaagggaa aacgctacgc cctcccctct agtcgcgtga tgattcatca
     5821 accttggggt ggtgcacaag gtcaggcagc agatattgca attcaagctc gggagattat
     5881 tcgcttgaaa aaacttacta tagaatactt tgcctatcat acagaaaaac caatagaaca
     5941 aattgcacaa gatatggaac gagacttgtt tatgtccagt gaggaagcta tggactatgg
     6001 aattgttgac catataatga agcggaggga agatggctct aaataaagat agaaaaacct
     6061 gttctttttg taaaaagcct gagggtccaa accgaatgct cataaagggc gctgatcaaa
     6121 gttatatttg ccagcaatgt agttctattt gtaactctat ttttgcacaa acagatagcg
     6181 atgagagtga atttatttta accgatattc ctactccaaa agcatttaaa gagcatttag
     6241 accaacatgt cgtcgggcaa gactatgcaa aaaaagtact ttccgtagcg gtgtataatc
     6301 attacaaacg gctttcgctg caaggttctc gctttgaaaa agatgcggac gaagtacaga
     6361 tagaaaaatc aaatattctt ctcatcggtc cgacgggttc tggcaaaaca cttttggcaa
     6421 aaactctcgc acaaaaacta caggtgcctt ttgcaattgc cgatgctaca acgcttaccg
     6481 aggcaggtta tgttggcgaa gatgttgaaa atgtcctact caagctcatt caagcagccg
     6541 gtggagatat agcaaaggca gaaaagggta ttatttttat cgatgagata gataaaatca
     6601 ctcgaaagtc tgaaaatgct tctattacac gggatgtttc tggtgaaggt gtgcaacaag
     6661 ctttgttaaa aataatcgaa ggcacaatag cgagtgttcc gccacaaggt gggagaaaac
     6721 atccaaacca agaaatgatt cctatgaaca ctgaaaatat tttatttatc tgcggtggcg
     6781 cctttgtagg acttgaaaaa attatagaaa agcgggttca tgaccatagc atgggttttg
     6841 gagcacaaac aaagttgcgt agcgaagagt caatgatgga gcttttaaac aaggttattc
     6901 cagatgacat tgtaaaattt ggtttaattc ctgaacttgt cgggcgcttg cctctctccg
     6961 tcgctctgaa agaacttacc gaagatgact tggtaactat tcttactgaa cctaaaaact
     7021 cgattatcaa acaatttaaa gcatcgtttt tatttgacaa tgtcgacttg cagtttaccg
     7081 atgaagcgat tcgtgccatt gcacatactg cgattaaaca taaaacaggt gcccgtggct
     7141 tacgttccat tgtcgaaaaa atgcttctcg atataatgtt tgaagtgcct tcgttaaaag
     7201 aaggaaaaag cttaactata actgaagatg ttgttttaaa tactgaaaag gcaaccattc
     7261 aattgttgaa aaaaaacaag gcctcttaag cttctagatt ttctaagact tcgattgttt
     7321 tatcggctgt tttagtccag gaaaaacgct ttacccagtc taaaccattt tctatcattg
     7381 tctcgcgtaa tttttcgtcg tttacaatgc gttcaaggtt ttcagctatt tctactgggt
     7441 tatgtgcatc aaaatagatc gcggagttgc cggcaaattc ggggagggag ccagcctttg
     7501 cacagagcac aggaattcca gttgccatgg cttccaagat gggaagccct acaccttctg
     7561 ctatcgaggg aaagacagag gcgtcagcgc aggaatagag ctcgggcaaa ttttcatggg
     7621 gaaaatgtcc tgttaaaaaa atatcttcag cgtatgcaga agcggcgaca gccttgtgaa
     7681 tgcgttcagc cttatctcca tcgcttccag agagtaccaa tctatgttca gattttgttt
     7741 tgcttttaaa taaatcaaat gcgtcgatga gctcgcggtg cattttatgc tcgccttgta
     7801 aacgagatac atagataaaa taggggcgtt ttatagaaaa aggttttata acaagggtgt
     7861 cccccgtcaa ttccctatgc ggataaaata aactatgatc gattccattg tgtataactt
     7921 cgattttagc gtcttttatg ccaagttgta ttaaatcctt gcgaatatac tgacttcctg
     7981 caattatttt atgtactttt tttagtttac gtacaataaa agctctttta aaaaaaggtt
     8041 tttctgcgag agcaagtgaa gcaatttctt gcacaagagc gaccgaggga acagaaaaac
     8101 gagctggcat aaaggaaatg ggatttggat agagaacaat atcgaatttt tgttttttaa
     8161 taaaagacag gataccgatg cgatgccaga gcttcaaaac aggttttttt gtagaaatag
     8221 gcacagcttc aaagctattt gcaaaggctt ctgagtaagt ataccggtca atttcttccc
     8281 cgaacaatat aaagttatgc ttcgtttttg agtcaatgtt tttaaataat gatataagat
     8341 atgagccgat tccacttctt ccatgttcac agccaaaggt atttattcca attttcatta
     8401 ttcgaattat agcatatatt atttgtctgt gctatacttt ttctaatgga aaatgataat
     8461 caaagtttta gttttttttc acttcaaaaa caatttatag acaatctaga caaacaaggc
     8521 attgtaaaac caactgcggt tcaagaaaaa gtgattcctg ccatccttga agggaaaaat
     8581 gttttttttg aatctgagac tgggactgga aaaacctttg cctatctcct tcccctacta
     8641 gaaaaagtaa gctttacaaa caaagatgtc caagttatta ttgtctcccc tactcacgaa
     8701 cttgcctcac aaataaaggg acaaatccaa atccttggcg attttttggt aaacctttgt
     8761 atcggtggaa cttctttgaa acggcaaatt gaatttctca aagaaaaacc gcaggttgtt
     8821 attggtactc cgggaagaat tttggaattg gtgcacttaa aaaaactcaa actcgctggt
     8881 ttacaagcga ttgtcttcga tgaaatagac aggcttatgg cgaaggaatt gcgcgatgac
     8941 acgatgcttc ttgcaagcct tacgccgagt gaatcgcaac tcatcgcctg ttcggctacg
     9001 atgaaagaaa atctttctga aaaaatagcc ctcagcgcaa aaaaagaaat tgaaagcatt
     9061 tatatagaac ctgaagatat tttacagaaa aatatagaac atattgcctt gttttcggag
     9121 caaagaaaca aggttgatgt cttgcgtagt tttctcgctg cggtaaagcc aaaaaaaacc
     9181 cttgttttta cctcaaaatt atctgatgtg caaatcatag caagtaagtt agcggcaaga
     9241 aaaatcgctt ctgcttcttt gtttgcgagt gcaagcatga aggaaagaaa ggctgcaatc
     9301 gacggtttcc gaagcgggaa atttcaggtt ttggtcacca gtgatttatc ttctcgtggt
     9361 ttagatatcc aggatgtaag ccatgttgtt caaatggact acccttcaaa cgaggtattt
     9421 tttatacatc gggcaggaag aacgggaaga agtggcaata tggggtataa cgttttaata
     9481 ggtgatgcct gggaaatgag agagttttca aagcttgaaa aaaaactagg gataaaaatt
     9541 accccgaaac aactttcaag tggaaaattg cttgcaccaa agctctaaaa tgagtatctt
     9601 agtaaaagaa tcttagtctc cagactgtat acagttttac atacataaaa agaggagtta
     9661 aaatgaacga agaaaaagaa acaagcaaaa agaagcaaat gaaatttagt ccaggaacat
     9721 ttattctcgg gattattgtt ttatttgccc ttattcttgt atttaccagt ttttttatag
     9781 ttgatgcaac tgaacaagcg gttataacac gctttggacg ctaccacaaa acgctcggct
     9841 caggcctaca gtttaagctt ccccttggca tagataaaag ctataatatt ccagtaaaag
     9901 tagtccaaac cgaacagttc ggctacaaaa ccattaaagg cggtataaca aataaataca
     9961 gcaacgaaat gtttaaagag tccaccatgc ttacggggga cttaaacatt gtggatgttg
    10021 aatggattat ccaataccgc atcgtagacc catctgcttg gcttttcaat gtcaaagata
    10081 agcaacagac aattcgcgat atttcgcaat ctgttataaa tacccttgta ggagatcgag
    10141 ctattctcga cgttatgaca actgagcgaa gtagtatcca agataatgcc gttgagatga
    10201 tgaatgataa cttcaaatct cttggtttag gtataaatat tattactgtg cagttacaaa
    10261 acgttgttcc tcctgcagat gtgcaagcag cctttgaaga cgtaaataag gcaagccaag
    10321 atatggagcg ctttataaac gagggtttag aacgctataa tgcagaaatt ccccttgcga
    10381 agggtgttgc agaccaacaa attcaaattg cagaaggcta tgcgataaat aaggtaaatc
    10441 gcgcacaggg tgatgtcagt cgctttcttg cagtttacga cgaataccga aaagctcccg
    10501 ctgttactcg cgaccgactc tatcttgaag caatggagca gattttcaaa gcaaaacaaa
    10561 atacaactct tgtagacaaa gaacttcaaa atatcctacc tataaaaaat ctcggaggtg
    10621 cacaatgaaa aaattcacta taggcttaat tattgttatc gctgttttta ttgtattcct
    10681 tatgatggga cccttttata tcataaacga aggaaaccaa gtggttgtaa cgcgctttgg
    10741 agaaattgtt gaaacgcata ccgaagctgg cttgtatttt aagatacctt ttatagatat
    10801 cgttaccacc tatccaaaac ttattttatc gattgatggg gactcgcaac gaattccgac
    10861 aaaggaaaat cagtttatca tagtcgatac tacgagtcga tggaagattt ctgaccctgc
    10921 gctcttttat cagtccttta aaacgcttga agcggcctat acgaggctca gcgatgttat
    10981 agattctgcg acaaggacga ttattaccca aaataggttg agcgaaattg ttcgctcttc
    11041 aaaccttata aatgaagaag agagtattga cccaagcata gaatcgctca gtgatgaggc
    11101 aacggaccaa atcagctctc ttataaatgt tgcgacaaag tctgagtcgg tgcataaggg
    11161 aagacggcat ttgacgaatg agatggctat agaggctcaa aaaatgctgc cacaatatgg
    11221 tatagaactt atcgatattt tgccacggca aataaaatac tctgatgaaa tgactgaatc
    11281 tgtctataac cgaatgatta aagaaagaaa taaggttgca caagcctatc gttcctttgg
    11341 tgaagggcaa aaagcagacc aactcggaaa acttgaaaaa gataaaaaac gcattgaatc
    11401 tgaggctttt agaacagcgc aggaaataaa ggggaaggca gatgctgaag cctcaaaaat
    11461 ttatgctgat gctttttctg tagatccaga attttacacc ttttggaaga gcttggaatc
    11521 ctaccaggaa acaataccgc tctttgacgc aagtttttct acatctatgg attacttcaa
    11581 gtatctgtat aatgcaagag gtcgctaaat gaacttaaaa gctcatgagg atgctgtcta
    11641 tttacacact gaaataggca gccgagagtt tgcacaatca aaattatttc ctttattaaa
    11701 ggaagcaggc ttccttgtga gctttaatga cagtgaaatt gattttacag aatggttttt
    11761 taccggcacc caagaggatg ctggtaaaaa gacaaaaata atgacgctca ttggtcctgg
    11821 ctttgaagct aagtcttttt atgagattct catggaaggc gagcgagaaa aaaaacaaga
    11881 tagtatatac cgtattcaaa gagcctatac agaagcgcta gaaaaaaaca tagccctccc
    11941 caatactggt cctatgggca ttttggtgtc agaagactct ctcttatttt taccacaaga
    12001 actctgtctt cgctctttta atgccttgtc tgaaacaaaa aaaagcgagt tctttggtcg
    12061 gtttaaaaac cgctccctca agggtcaaga aagcatagac ttttgccttg cagtctacat
    12121 atatatctat cttacagggg acttccccta cccgagtctc gatgaagaaa aacggcaaga
    12181 acatatacaa tacgcagaat gtataccact tcatttatat aatccttctt ttccacaaga
    12241 tatgattttt tcagtagagt ccattcttca agggaaaaaa gaaaagccat ccttgcccct
    12301 cctcgataaa agctacctgg aaccagagat aaccgaaaaa aatctagata tattgcgaga
    12361 aaaagagcga aaagaatggt tagaaaaaaa acaaaaaaga aatagtcgta ttattttctt
    12421 aaaaaaacat gcaacaaaac tcatcatttt tccagctatt ttttttctcc ttgccctcac
    12481 catcataggt tttatgagag ataaaaaagc tggtccaaac tccctcggtt taagcgatat
    12541 tgaaacgatc gaggcctatt acacggcggt taatacgctc gacctgagcc tttcatcaaa
    12601 tctcttatac aaaaaaacaa agtcgccata tgaatcgatt atagctacct atcacgtagt
    12661 caaaatgact agagagtctt atgagcgcat acgccccttt gtgcacccca tagaaaaaat
    12721 acaaaatgca agtttggtag attctggtat gtttgggatt agccatctaa aaattggaga
    12781 tacgctgctc aatccttttt ctcaaaaagc aagtgcacaa aataaaattg cagtacctga
    12841 tatagcagat aatgaaaaga gacaatatac tgtcaaattt tattttatca aaaatgaggg
    12901 tgaagatgac ctcgttgtag aattttgcga agactatgta gaacttgttt ttcataaaga
    12961 tcgctggctt ataacgaaag ttttgccctc gtcaaacata caaattgaag actatgttct
    13021 ctttttagaa aaactagaaa acattgcaga gctaccattg gaagaaaaaa taaagctgct
    13081 cgaaaaagaa tatgtatgga tgccaagtct agaagaacta tattttatac aaagtgaaag
    13141 agaagatgat gagtgagaaa gcctataaaa gacccagctg ggacgaatat tttatggaaa
    13201 tttgtcaaac tgtggcaaaa cgtgctacct gcgacagggg acgaagtggt tgtgttatag
    13261 caaaagaaaa tcaaatcctt gttaccggtt acgttggtag tccaactggt ttgccgcatt
    13321 gtgatgatgt tgggcataag ctaaaaaaaa tggtgcatga gagcggcgag gtaacgcagc
    13381 actgtgttcg cacggtgcac gcagagcaaa atgctataac acaggcagca aaacgaggga
    13441 ttgccattga cggagcgacg ctctactgca agatgacgcc ctgtagaacc tgtgccatgc
    13501 ttataatcaa ttgtggcatt aaacgagttg tttgcgaaaa aaaataccat gatagcaaag
    13561 attctcttga aatgttctcg caagcaggtg tagaaatact gcatttacaa gaagaagtac
    13621 aaaaatacga gaatatgtaa aagacctctc aaaaactgag tctttaagaa cctataaata
    13681 aacaaagggc gtaaacttct tttactccag cttctttgag cacgagcgcg caactttcca
    13741 aggttgcccc agttgtcata atatcatcga ttaaaacaac ttttgacggt aggtctttta
    13801 taccatcttt taaagcatag actcgaccga ttgtatctaa gcgctcctgt cggttcattt
    13861 ttttttgttg tctacggctt acacgtttta aaaggtttaa gattttatgt tttttattta
    13921 acgagagtat ctttgtaatt tcgtctattt gatcccagcc tgtttttcgt atttttcccg
    13981 ggcgcggtgg tatgggaaca agggctatgc caaagaaaaa acgctccaaa acttctgcat
    14041 acatttttgc aataaccggt gaaaaagaac ggtttcccag aattttccag taaaataaaa
    14101 cttccttttt ccataaacga taggagtgca gtggtaggac aaaatcgaca tgttccagca
    14161 aaggtgaatc gcgacaactg agacaggttt ctttttcact tacaagaaaa ctaccacatt
    14221 tattgcagta tttagttaaa tcctttgggg tttccataaa atctgcaagg caaggattgc
    14281 acaataaaaa aacgctcctt tctccgcaac gcaggcattt ttcattccca caaagaaagg
    14341 ataaaaagcg atggagcttt tctttccatt tatatatact aataaggctc ataccttatt
    14401 agtggcgttt tataggtact tttatttaat tataaagaaa aatttatcaa tatacaaaat
    14461 tctatcactg gcttgtttcc aaaatgcact cgaaggatag gcgtcgacaa tggtttggta
    14521 tgattctcgt gcaagctgta tgtttcgcat ttttccgggt ttttcgtata attgtccacg
    14581 caaaaaccaa gcctcgtcag tattgtctaa actgacacgt aaaaattcgt cgagatataa
    14641 aagggcatcc tctggcgcat tatttgccat ggattctttt gcacttgaca aaagcatttc
    14701 gctcggcatt gtttcaactg gcggtaaaaa agcgagttct tctagctcag gttcatcgct
    14761 caaagctgac tgagaccctg caagaagttt ttgcccttcg ttttctgcag ttatagcctc
    14821 tggcgctttc aaagcatctt cgtagtcata gctgtcaatg agtttttttt cagaatcaag
    14881 ggctctttca tcacttatag tcaattcaat tgtatcttcg atatatgatt gtgaaagtgg
    14941 atccgattta taaaactcaa ggcgagtaag ccctggtttc gttgttttca aagtaaaaag
    15001 tgtatcccca tctactattt ttctgcccac ataggataca aaattttcct gcccttcttc
    15061 tcctatataa atccaaccac ttcctggata gcgtatttct aaaaactgat tgttttttgc
    15121 atcagccttt gttttacttg caagctcctt tatttctggt aggctttttt cctctggctc
    15181 agcaaattgc gcttcttgaa tgttctcggt ttttatttcc tgtgcttttg tcgcttcctg
    15241 aatcggggga ctggggcttg gagtttgcac tactggtgct tgtgtgattt gtggcgcttg
    15301 tgctagctcg gcaacttgcg gactagcagg cggacttgga acttgaatcg gtgttctttg
    15361 taaaccttca atttccgtta tcacctcttc ggtactcggt actatatcag ttttttctgt
    15421 ttccaaatca ccttgccata ttttcgcttc tgtttttact tcttgtgttt gaacgggaat
    15481 aagttttttt tcacttttat cgctatctgc aacttcatcg gaaacaacat tgtcaaaaac
    15541 agcttgattt tcatcgctct ctaaatcctg ttctggaaaa aaaccaagac tttctacgat
    15601 tggctcttca aaaagaatgt cgtttttttc ttcgatagtt tcaaaatctt ctccaacaag
    15661 ctcatcgggg ataggctttg tatcggttga attatttata tcacttgtat cgtnnnnnnn
    15721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtaaaaa aaagaaaagc gaaagcaaaa
    15841 aaaatgcttc tcaaaaaaaa gttttggcta tgtattttca tggacttccc tccattatgc
    15901 cttgaattag acgggtattg tcatcatgca gtttttctat catatattcg tttggtatag
    15961 taaaccattc ttgaatatct tcatcgctcc atttttcttt ttgttctcta gaaagataat
    16021 attcttcgcg aaaaagtggt tcagctggcg gaagcaagtc ttgatctaaa tcaaagttgt
    16081 agatttccct ttggggaatc accgcttttt ttataaatac ctttccatat acggttacac
    16141 ttaaaaaaag agccacaagg ataatcgata agacagtaaa gaaacgtttt ggatattttt
    16201 tataaaaaac atcgagacct gttttgtgca agatactata agttagattt gcaataaaat
    16261 cttgaaaggc gctaaaaact tgttttactc gatcaatcac gtatcatcct tgtaaaaaac
    16321 taatgcctag tttatctttg ctcgttcctc actcgcttgg gtggatgcgg tggcatatac
    16381 acatcctctt ctaggggaac atcttcctct tcaaacggtt caatatacgg tatggaggag
    16441 tctgaattag cgccctcatt ttcaagacga atcgtaataa ccttacttac gccagattct
    16501 tccatcgtta cgccgagcat tgtgccggct ccggttactg tttttttatt gtgtgttata
    16561 acaatgtact gacttacatt tgcaaattca cgcaatgtat gcacaaagcg aagaacgttt
    16621 tgctcatcaa gggctgcgtc aatttcatcg agcaagcaga acggagaggg acgaaccatg
    16681 tatgttgcaa ataaaagtcc gattgcggtc attgtttttt caccaccaga taatagggct
    16741 atgttctcaa gtttttttcc cggcggttgc gcaaaaattt ctacaccaga ttccagtacg
    16801 ttttgtgggt ctgtgaggcg cagttctgcc ttccctcctc caaaaagacg acggaacata
    16861 ttgtgaaaat tcttttttat attgttgtag gttgccataa aaagctcagt agattcagcg
    16921 cgaatttctt cggtaatgcg ttttaagtca tctcgcgctt ttacaaggtc ggcaatttgc
    16981 tttgttaaaa aatcatagcg ctcttttact tcaagaaatt cttctggcgc cataaggttt
    17041 accgagccta tgtcttttaa ttcttgtcga gcctttgaca aagattctcg caaactcata
    17101 atcggcatag ttatggtata cattttttct tcaaactcaa gcaaatctcg tgaatggctt
    17161 tctctgaagt tatcttttag gttacgtatt tccgtatcta tggtaacaat gtcgagattt
    17221 attttttcta gttgtgcttg atatttaccg agctcttgtg ttttcttttg aaccgcatct
    17281 tgctttccag aaacatcttt atttttggag ctaatgctgt actcgaggtt ttcaagctct
    17341 tttgtaagcg tccgtcccct atgttcaatc gaggcaattt ctccttcaag ctctatgatt
    17401 tgctcgttta tttcttctga gcgttttttt tctgtaaata cttcgttttc taaatcggat
    17461 aaggcatttt cttggttacg tagttcgcgg cggattatgc gtatttggtc ttctgaagct
    17521 tgcacctcac ttctcatctg cgcttggttg atacgtagtt tttcgagcgt ctctcgatac
    17581 tcgttaatct tttgagataa aaccttgttt tcttcttcga gctttacaat ctcctgcttt
    17641 ttttcttgca ttttttgaag cgtctgtttt atcttttcat cgatgcgtct ttttttggta
    17701 ataatacctt ctggagataa aaagtcatct ataaaatgta agcttgtttt tttatactca
    17761 tcaaaggcgt tttccaaatc tttgagtgag tcttcaagtt cagaaaaaga cgaaagcacc
    17821 atctgtgaaa actgggaaat atcgctctca gtatgttctt gaatagcaga aaaatcttgc
    17881 gagattgttt tttttgaaga agcaaggcgt tttatgtttg ccataagagc tgtaaatttt
    17941 tcaagggcct cctcgtgtgc ctttgaagaa taacccgcat cttttaattt ttggtcaagt
    18001 tcacttacaa tgtcttcggt aattgcctcg agttcggttt gaaagcctcg gttttctttt
    18061 tcaagactat ctatatcgcc ttcgagtttt tttacatcat cagagttttt ataaatttga
    18121 tttgaagcaa gttttatatt gctttcaaat tctgaaatat tgtttacaat gtcatcgatt
    18181 cttttgtgaa tttgatgcaa gcttgcttct ttttcatcta tttcttcttg gagattttct
    18241 atttgctctt cgaggttttt tttccgattt tccaaaaccg caatttttgc ctttgcttct
    18301 gcatgttgct cggtaacaat ttttatttga tcttgttttc ctttttgctc cgcagcaagt
    18361 ccaaaaattt cttgattcat ggcgatgagc tttgtttgca tttcgtttac cgcatccatg
    18421 tgttcggcaa cggaagcatt tatctcatct atttcttttc gtgcaatatc tcttttttca
    18481 cttgcggttt ttaggtcttc ttctttgcgc cccttgtcgt ttataaagcc ttttaaacgc
    18541 aagagctgaa tatcaagctc aaagttaaaa atagattcac gtaaggcacg gtattggtgg
    18601 gttttatcgg attgtatttt gagggagtcg tgacttcgct ttacttcccc cagaatgccg
    18661 tcgacttggc gcatattttc ttcagttcgc tcgagtttac gctcagcctc cgcccgtttc
    18721 gccttgaatc gagttattcc cgcagcttct tcgaataaaa aacgccgctc ttctggctta
    18781 ctcgacaaaa tttggtcgat ttttccctgt tccatgacag aataagctgt tttacctaca
    18841 ccggtatccc aaaaaagttc tcgcacatct ttgagtttta cctgcgtatt gttgataaag
    18901 tattcgcttt cacctgagcg atataaacgg cgcttgagtt gaatttcgct ggtgtccagt
    18961 ggaagtaagc cgttatcgtt ggcaagggta agcgcaactt ctgcaaaatt caatgccttt
    19021 cgcgtttctg ttccattaaa aataacgtct tccatttttt cagcgcgaag tgaccttgca
    19081 gcttgttcgc ccaaaaccca ttttaccgca tcgacaacat tgcttttccc acaaccattg
    19141 ggaccaagca gggctgttat accagtagca aaatcaattt tagtacgatc ggcaaaggat
    19201 ttaaagccaa acatttcaag actttttaga aacacgaaaa acctctatat tttgtaagaa
    19261 tagtatatgt acactttcag tattgttcaa ttttacctgt atttttgact aaagtcaatg
    19321 aactacttga aaagagtcct taagaatact atgatatccc tatgttagac tatagattta
    19381 taaaagaaaa tattgatgcg gtaaagcaaa atattaaaaa cagaaatatg acaagtgatg
    19441 cggatttggt tgttgaatta ttcaataaaa gaacccaact catttctgcc ctgcaagaat
    19501 ttcagcaaaa acgcaatgaa aatgcgactg cgatgaagca aaagctggat gaagcgcaaa
    19561 gaaaggcgct tatagaagag ggaaaggctt taaaagaaaa aatttctctc atggaagcag
    19621 aattaaaaga agctgaggaa gcgcttgatc gagaagggaa aaaaattccg aatatggcac
    19681 atcctgatgc accgattgga agtcttgata cagaaaatct tgaagtaaag caagtgggaa
    19741 aaccacgtga atttagcttt gtaccaaagg atcatgtaga actcggacaa aatctcgacc
    19801 tcatcgattt tgaagctgcg acaaaggttt ctggaccaaa gttttattat ctaaaaaatg
    19861 aggcggtttt tcttgagcaa gccctcacta tgtacggctt acaaatctta aaaaagcacg
    19921 gctttacgcc ctttattacc cccgatatcg cgcgagaaga aatcttaacg ggaattggtt
    19981 ttaatccccg tggcaacgag tcaaatgttt acagcctcga aggcgaagat gcttgcttgg
    20041 ttgcaacagc ggaaatcacc ctcggtggct atcataaaga cgaaatcctt gcaaaagaaa
    20101 ggcttcccct tcgctactgt gggctttcac attgttttag acgagaagcc ggagcagcag
    20161 ggcagttttc caagggcctc tatcgcgtac atcaatttac caagctcgaa atgtttgtct
    20221 actgtactcc cgaagactca gatgcaattc acgaagaatt acgactcatc gaagaagaaa
    20281 tttttacggg actgggtatt cccttccgtg ttgtagatac ttgcacagga gacctcggag
    20341 cacctgccta tcgcaaatgg gatttggaag catggatgcc cgggcgaaac ggcggcgaat
    20401 ggggagaggt aacttcaacc tcaaactgta ccgattttca agcgcgtcgc ttaaatgtac
    20461 gttataaaaa cgaagatgga aaaaacacct ttgtgcatat gctaaacgga accgccattg
    20521 ctatgtcccg cgccttggtt gcgatttttg aaaactatca aaacgaagat ggctctatcg
    20581 atattcctga agtacttcaa ggtctttgtg gcttttccaa aatcgaagca aaataaaacg
    20641 gaatagaaaa aaagataaaa tattttttaa aaagccggtc taaaaaaaac aatctagacc
    20701 ttaatcaaat tctgaatcga agccgtagag tttttcactt aaaaacctac ggttttcttc
    20761 ttgtaaagag cttatatggc aaaaagctat ttcctttttt tgcccttcaa ctgaatcaaa
    20821 taaaatctgt tcaacaaaga tagtagagtc gatatggcga gtcagccgaa gaaccggtat
    20881 ttcaatagtg ataaaatact cgctcttttc atgcataatt tctcggatag attcacttgc
    20941 aaacacaata tgtgtatctg acaaaaaaag tatttccaag ggtcgcgccc tacccgcaaa
    21001 agactccgcg cctcgctcct ctttttgagt gagatagatt gctgcactta taagcggata
    21061 aaaaggcttg gtatctgcag cttttttttc tataaacaat tctgaaagag aatatgtttt
    21121 ttgatagaga ctttccacaa gactgagaaa ttttttttca gactctgatg aaaggtcctt
    21181 ccataaatcg atagaaaaaa gcggaataga agccttggaa tgaaagggca caaaaagtgt
    21241 cgattcattt tgtaagccaa aataaaactt gccgtgaatt cttttttctt gtgaaataga
    21301 atcgtctatt tgtttaaaaa tatagggcga gattacaagc gcatagcctt ttgtaagatg
    21361 tgaaaactga gattcaaaga tgagggctct tcctttgaag tgaaataaaa ccgctatgga
    21421 agtgccatga ggaatactaa aggaaaaatc gctaaagggg aaaaaaagaa tacccttatt
    21481 taccacctga taggattgtg gcgctatagt aatttgtgga aactgatagg cctctgcttc
    21541 atgtggtaga tattctgtag aaagtggttt aaaaagcaat aagggttttt catcgatcaa
    21601 atgcgaaaga attaaatctc gttctacctt tgaaagataa ttattcatat ataatgtttc
    21661 ctatttggat ttgttgaata cgtttttttt cttcgctttc taccagaaaa aaaacaagct
    21721 tgtagtgggc ttttttatta tcctgcgttt tatcgctata tattttaaca ggcacttcta
    21781 ggtaatttgc cgtaggagag ctgagaggaa aggcttctcc gaaaatccag gaaaccggta
    21841 agggatagtt ttctataaaa tctgttagta taatagaaag atagcgagca tctggtaaaa
    21901 aggcttcttt ggcatagcga gctgaggcaa gttctttgag aaaggcatcg agaaaataac
    21961 gggtttttgg atcgaggctt cgtgaatcca gactaccaaa tccttcgata tagggataaa
    22021 ttgccctcga tgtgtttgag tcatgtaaaa aaagcatatc tgaagtaaga agggtatcgc
    22081 ttgtatccgt tttttttgcg gagctttttt tatcggcctc tgtggtgtat aaaatatctg
    22141 cttctatttc ttctatccat ggaatgtcgc gcgttttttg attaaactca ttttttaaac
    22201 tcaagacctc gtcttcatat tgatagaggt ttttatcaat cttgcaagaa cataaaaaaa
    22261 gagaaaatag acaaaataat gcagaatatg acaatttcac aaaaaaagta tactgtattt
    22321 ttgaaaaaat aaccagtgta gacataaaaa ctaaatcctt ataaaataaa gccattatac
    22381 atgggaggct tttttaaatg gctttaaatt tatcacaaaa aatattggcg gcacatcgtg
    22441 tatcaaaaga cggaaatgat attgcaataa aaatcgatca aacattgacc caggatgcaa
    22501 cgggtactat ggcgtacttg cagtttgaaa cgattggtat tccgcgggta aaaaccgatg
    22561 tatctgtgtc atatattgac cacaataccc tacaaaccga ctaccgaaat atggacgatc
    22621 accgatatct acaaagtatt gccgaaaaat acggtttgtg gttttcacga agtgggaacg
    22681 gcatttgtca tcaagtgcat ttggagcgtt ttgggaaacc gggggcaacc cttcttggaa
    22741 gcgactcaca cacgccaact ggtggtggac tcggcatgat tgcgatcggt gctggtggcc
    22801 tagatgttgc tgtcgctatg ggtggtggtg cctttcactt aacagagcca aaaatatatg
    22861 gggtaaaact cacaggcgtc ttgaaagctg gcgtttctgc aaaagatatt atcttggaag
    22921 ttttgagacg agaaaccgtc aagggtggcg tcggtgcaat ctacgagtac tttggtcctg
    22981 caattgcgca attgacggtg ccgcaacgag cgacgattac caatatgggt gcggaacttg
    23041 gggcaacctg tagtattttc ccatctgacg aagttaccaa aaactaccta gcagcaatgg
    23101 gtagggaaaa agattggatt gccttaagtg ccgatgaagg ggcgagctac gacaagctca
    23161 ttgaaataga tttatctagt ctcgaagctc ttgcaagtca accacattct cccgatgcag
    23221 taacagaggt aaaaaaactc aaaggcaagg cagtgcaaca agttgttatt ggctcttgta
    23281 caaatagctc tctcgatgat ctttcacgag ttgcttctat agtaaaggga aaacatatac
    23341 atccaggcgt agaagctggg attgctccgg gaagccgcac taccctgctt ttggcggaga
    23401 aaatgggcat cctcggcgac ttaataaagg ctggatttag gatactggaa tcagcttgcg
    23461 gtccctgcat cggaatgggc tttgcgccat cttctggcgg agtttcctta cgaaccttta
    23521 acagaaactt taagggcaga agcggcacac ccgatgcgct tgtctacctt gtctctccag
    23581 agactgctgc ggcggcggca attactgggg agataacaga cccgactgag ctttctgtct
    23641 cgtatataaa tcaagaaaaa agcttactcg acggcgacaa tcccttgctt tcatctgagg
    23701 atatagcggc gataagcagc gacgatggta tgctcattgc acccaaaact ccagaagaag
    23761 cagcaaaggt tgagataatt cgtgggccaa atatcaaacc ctgtccgctt tcaccacagt
    23821 taaaagcttc tttgacgctt cctgttttac tgaaggctga agataatgtg agtacagatg
    23881 atataatgcc tgctggggca aaggttttac cacttcgctc aaatatacct gagatttcaa
    23941 aatttacctt tgaacggctc gatgagtgtt tttattcacg tgcaatagaa aatcaaaagg
    24001 taaaccctaa tggctgcatt gtcgtaggtg gagaaaacta cggtcaaggc tcatcacgcg
    24061 aacacgccgc ccttgcacca atgtttttag gcgtccgtgc tgtattggtt aaatcttttg
    24121 cccgcattca caaagcaaac cttatcaact atggaataat tccccttgta ttcaaagata
    24181 aagaagacta tgaaaacatc gcctttggag aaaaacttgt cttggaagat atttttgaaa
    24241 gcttggatac gggaaaaaac tttgttttga aaactgcaag cggaaaaacc atagaagcga
    24301 taaacgacct tgatgaacga agcaaggcaa ttctcaaagc tggtggactt gctgcctata
    24361 caaaatctgg cggacaataa aaaggagaaa tcatgcaagc ggttcgagac ccattgtgga
    24421 aacatatttt tttaccggat gccttatacg aggcaacaaa cacaaaggat tttctacgct
    24481 tgcatggcat aaagcaactt ggaccaagct acctcgtgta tccgggagcc acccatacaa
    24541 gggctgcaca ttccttcggc gtctatgaag ttgcctcacg actgataaaa aacctcattc
    24601 aaaaaggagc gcgtaactgg gtaagtcgtg aaggcatgca gtcttttttg gcggcaagcc
    24661 tttttcacga cctagggcat tttccctaca cccactcgct caaagaactc cccctactcg
    24721 accatgaagt gctcaccgga gagataatcc tcaaagaacc acttcgctcg attataggaa
    24781 aaacaggtgc aaaccccgaa caagttgccg ctattgtcga cacaagcaaa gactgcagcg
    24841 acgtagaaac acttttttat agaaaactct tatccggtgt cttagacccc gataaactcg
    24901 actatctcaa ccgcgatgcc ttttattgtg gagttccata cggcatacaa gatacagaat
    24961 ttgttttggc aaaactcata ccacacaaag aacgcggcat aattattgat tcttctgcaa
    25021 tgctttcctg cgaaaacgtg cttttttcaa aatatttaat gtacaaggct gtgtattggc
    25081 ataaaaatgt acgcatagca accgctatga tgaaaaaaac actctacacc gccttgaaag
    25141 aaggcatact acaagcaaaa gaattgtata gcatagacga tcagggctta tatagcctcc
    25201 ttgagtcaaa aacctatttt gatgaacttg aatgcgcaga tgcggtacaa aacaaggctt
    25261 ttttcaaggt actctacgaa tgcccctata atgaaagcca caaactgcat agagatttgg
    25321 aagatttaga aaaaagagaa aagatagaaa aagagattgc agaaaagata ggctgcaaag
    25381 cggcacaaat ccttatcgat gtacccgaac gtatttcttt tgaaagtgat ttatatatac
    25441 gagatgaaaa aacgagtttt tctaaaagta gcacggtatt ttcggagcat acggtagagc
    25501 gatttacaac aagtttacga aaaattcgcc ttgcggtatc acctctgttt ttatcccaaa
    25561 aaaccgataa agaaactgaa aaaaacataa aattgattga agaattcttt ttcttggact
    25621 ataatacata gcatggaggc ctcatgaaag tcctgaatta tatggaagac cttgtaataa
    25681 caagaacaaa tgaactttat gatgaagtca aagaaatgga tgcaccctgg ctcaattgtg
    25741 attgcgaaca ttgtagactc gatactatta gttatgcctt aaaccgtttg cctccgcgct
    25801 atattgtctc gggaagaggt tttatccaca acaccgttga ccaagacagc caactcaagg
    25861 ccgatattga ctcccttgtt attgaaggga tgcacacgat caatactacc caaagaccat
    25921 atcacggaac agctaaaggg aaaatgaaag gtcaaacagg accttatttt aattttccaa
    25981 cctttattgg aagtgtttta gacgggacga catttgaacc cttgagcgat gtagaaataa
    26041 tcttaaaaca agacaatgta aaatcggaaa tggttgacta tacatgggca aatccgtata
    26101 taagctccaa attgaccaag ggtacctact cttttttagt aaaatccgag gaagcagaaa
    26161 aagaaaatat aagcaaggtt tttgtcttta cgcttgaagc aaaaaaagaa ggttatgaaa
    26221 gcatcacata taactttagt attccgctta taagtgaagc aacggcaaag gttgaatcaa
    26281 agtcgaccta ctcattaaaa attcaaaatc tctttatgtt tgaagaaggt agcacaaacg
    26341 agcttgaata aatctttgta aaaaagagca ataccatttg actttttttt aataatttgc
    26401 taaagttaat aaatcacggg atgtagccta ggggctaagg cgtctggttt gggaccagaa
    26461 gatcgggggt tcaaatccct ccatcccgac ttttaattac ttaatatata tagaattaac
    26521 aaaatacata ttctccgctt tgaaaaaaat gccatgaaaa tgccataaaa caaggagttt
    26581 ttgagaatat gtacagaatt tacaaaaaac agatatacag tcgaccttct tttctaagcc
    26641 acctgtgcgg tggtcaacaa atatagcttt taagtccgtc aattgttcca taacttacag
    26701 tataaagcta aaacgcgctt tttacaattt ttaaatacag tttagtccct ttttctcctt
    26761 cttctcaaat ctcacaaatc tctcgtagtt taacacacaa atgaagcttt ccctttg
//
LOCUS       DAOSSW010000013        26340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_2045_length_26340_cov_7.226022, whole genome shotgun sequence.
ACCESSION   DAOSSW010000013 DAOSSW010000000
VERSION     DAOSSW010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 26340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26340
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2045_length_26340_cov_7.226022"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="PKW26_02260"
     CDS             <1..1331
                     /locus_tag="PKW26_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92422.1"
                     /translation="SKSIFLQSQNFSGGLHYPLMKDSAVIFVSSFFDHDSLFVSPFET
                     IFKDARFIKSEKTFDDADVILEEVLAEGFERESMEAIASIESVDDEARPETTSLDAYK
                     TKKYNPIAYMTKGIFLPTLSLEIIDDTSDNDEFDLSLSFGFMYLTGDPADTWQILIQP
                     SYDFTNEKIYLQTEIATTAFPLPLSLEGISVFGLDGSVQNDYTLSSAIEIPLGNSPYS
                     FNFSTALFLSHGKKNAESAFFNFLHNGNILSFSSIRNANLNPFALKGFELGASLWFFD
                     TKDPEFSLDTNYAYNFFASYCLPQVLPIQNPIGFTYNLPTVFEASLFTSQNVYEEEEA
                     LWSASATTTLFAFDFQNALSNIYIQRFIFEAEYACMQGLDYIDYKNPLDNFSHIERNL
                     FKDSVAISAHFTSSVIVGMLLTQLKIDLGATFRYHIKDRKPEWSLLIRFH"
     gene            1395..2564
                     /locus_tag="PKW26_02265"
     CDS             1395..2564
                     /locus_tag="PKW26_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOQ92423.1"
                     /translation="MKSINAPSDINVSRILRLVWQKREISRIEIASQLGIDKSTVTKI
                     VSSLIDMGLISELSEGNTGPQGGRKPIFLEISPSFACVGGVEINSGRYVCCLLDMYGK
                     VLFQYAEAIEPGSYEIDMYQGIFLKAFETLSSEAEKKGLPLVGIGVGFPGIVNSEEGI
                     ITQSVPLMIDEAYAFVEEVSKEIAIPLFIENDARCCCYGEMLLAKTHDVKNMLYVLAE
                     YRILQPTKSSQKNLSVGLGIVFDGLILKGADFSAGEFRSLLWDEGNKGQFYSGEKKLE
                     QLDEGAVQPVFFELAQHIAFLVNTLNINQVYIGGIDKKYAEQLIDSINTRIRFQWPYK
                     AKQNYKVSLASLEMFAVSYGAAAMFLEQIFALPNLSLKSGMGESVLKTFESLKKN"
     gene            2744..4048
                     /locus_tag="PKW26_02270"
     CDS             2744..4048
                     /locus_tag="PKW26_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOQ92424.1"
                     /translation="MRLSKKVLLVSLVLVLAAGMVFAGGSKDTAKKQGKVLTIYCWND
                     EFQTRFNDYFAAKGLVPDGIEVNWVITPNQGNAYQDKLDEALLRQNSASERDIIDIFL
                     VEADYALKYVDTPFTLDVFKDVGLTSADVANQYKYTKDIMTDSKGVLKGVSWQACPGG
                     FIYRRSIAKAVLGTDDPAKVQEALSSWDKFDAVAAQAKAKGYFMLSGFDDAFRVFSDN
                     AKTPWVGAGDKIQIDANIERWIEQTKTYTDKGYNNKANLFSAESWGGGMKDGKVFGYF
                     GPSWFQDFCLAPAVDTAGDWAVCTGPQGFSWGGTWICAAAGTDNIDLVKQVMLTLTTD
                     KDTLVAIAQEAGDFTNHEGAMAEIAKSSYGNPFLGGQNHIAYLLESAKKIDRSSMGPY
                     DQGMTETIQASFADYFNGSVTKGQAWDNFYKAIIEIYPNLKK"
     gene            4125..5057
                     /locus_tag="PKW26_02275"
     CDS             4125..5057
                     /locus_tag="PKW26_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ92425.1"
                     /translation="MTKTSRPQKKRLVDYSKWGYFFIAPFFIIYVIFSLIPLLSTFVY
                     SFFESYRIGLQQVGPNFVGFANYKAVLESDFLKYTMNTLLLWILNFVPQILVSLVLAV
                     WFTSSSLRLKGQPFFKTIIYMPNLIMASAFAMLFFALFSDKGPVNIILEGMDIEPIRF
                     LTKISWTRALISIMNFLMWFGNTTILLMAGILGIDESLFEASRIDGASALQTFFKITM
                     PLLMPIFIYVLITSMIGGIQMFDVPQILTNGSGNPNRTSMTLIMYLNRHLTSRNFGLG
                     GAVSAFIFLMTSVLGFFTYRSLMSQYRKSPSRKS"
     gene            5072..5947
                     /locus_tag="PKW26_02280"
     CDS             5072..5947
                     /locus_tag="PKW26_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ92426.1"
                     /translation="MTTETTIYRKPKGNVFLQLQRRLAYLFLLFLAAICIFPFLILLV
                     NASRSNAQIQVGFTLLPGKFFVTNFLNLVQDPNMTILRSLFNSVFVSSATAFFSTYFS
                     AMTAFGIYMYRFKGRNFAFNFIMLVMMVPGQVSALGFIQMVTKMGLYDTFLPLIIPAI
                     ASPVVFFYMMQYMESVLPFEIVESARIDGANEFFTFNTIVLPILKPAIALQAIFSFVG
                     SWNNYFMPALILSSKNKKTIPILIAQLRSADYMKFDLGKVYMLICLAIIPLLIVYLIL
                     SKQIIRGVTMGSVKG"
     gene            complement(6027..>7283)
                     /locus_tag="PKW26_02285"
     CDS             complement(6027..>7283)
                     /locus_tag="PKW26_02285"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOQ92427.1"
                     /translation="YENILMFERDFPNVMEIRLEQNYRSTETILAAANGVISHNTNRK
                     DKSLWSGNGSGKPIEVYMPEDEHDEASFIAEMIQSLRMSEGRKFGDFGVLIRANTMTR
                     AIEEAFLAENIPHSISGGTSFFQRKEIKDIISYLRLISNHDDDINLLRIINTPRRGIG
                     KKTIEQISAISTSQKCSLWTSINALIAASESPLSDRAKADLVEFVELIGKQRSLLLSG
                     GKGLSKKVRAMVDEINYMDYLMAEYQKNEKAARFKFLNIEHLLSGIDSWENNPDTFDS
                     GLYAYLNRITLLSRDDMEDEEGLLGKVNLMTIHASKGLEFPVVFIAGAEDGLIPHARS
                     VEEGDGNIEEERRLFYVAITRAQDKLFISSCRRRRKMQTVVECTPSPFLDEIPENLVS
                     YHEPKGAVSDAEAIDFLAKMKNRFVM"
     assembly_gap    7284..7383
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7384..7865)
                     /locus_tag="PKW26_02290"
     CDS             complement(<7384..7865)
                     /locus_tag="PKW26_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOQ92428.1"
                     /translation="MSTMRLNEQQDKAVKTIEGALLIIAGAGSGKTRVITNRIVHMLE
                     MGIPQSQILALTFTNKAAKEMQERIRELTGKKLQNLSISTFHAFGVKILRQDIETLGW
                     RENFSIYDEVDKNQLIKETARELGLKTEGLDIYKIANLFSNIKIGRWTWEASNDAYKQ
                     L"
     gene            complement(7843..7974)
                     /locus_tag="PKW26_02295"
     CDS             complement(7843..7974)
                     /locus_tag="PKW26_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92429.1"
                     /translation="MDAKSNVPVLTISSWASFAKSIVPLYQQAFLIMIIVYVNYEIK"
     gene            8125..9453
                     /locus_tag="PKW26_02300"
     CDS             8125..9453
                     /locus_tag="PKW26_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexokinase"
                     /protein_id="HOQ92430.1"
                     /translation="MTVAQKVNAFLGKNGFTCGGPNINAVIDSLIWDMKKGLNEGRST
                     EGAFVASQDMIPTFAMPPTERIVNKNVIVIDAGGTNFRACLVHFDAEGTYTISDFKKT
                     AMPGIEREYGKKEFYDTIAKNLDHLKGKADSIGFCFSYAMSITPEGDGRVLDFSKEIK
                     AKEVVGSLVGESLSAALVERGWEKPKKIILLNDTAAALLSGAASAKPGRLFSSYVGLI
                     LGTGMNAAYIESNPIKKIAGQKAPESQIVVCETGKFDKLPQSLFDIAYDKTTNTPGMY
                     IIEKMCSGAYLGPVTGFALKQAAKDGLFSEAVAKDLLLIEKFELKDMDQFFFAPYNTD
                     TLLGAIMAKGNQDDYDTCYALLDAFVERTARLAASIIAACVIKSEKGTQASLPVCVVT
                     EGTTYFKTHNLQDRVKGYLNTVLMEERGLSFELVSPESAITLGAAVAALS"
     gene            complement(9505..9852)
                     /locus_tag="PKW26_02305"
     CDS             complement(9505..9852)
                     /locus_tag="PKW26_02305"
                     /inference="COORDINATES: protein motif:HMM:NF019401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HOQ92431.1"
                     /translation="MQTILPIIKMVKLGINVLSVIILCIGVVFCARDLFLILIAKIPK
                     SEKISRLQYMKTELGSYVLLGLEVLIVADIIETIINPTIDDIILLAAIITIRTVVSLF
                     LNKEIKEIREAAH"
     gene            10134..10601
                     /locus_tag="PKW26_02310"
     CDS             10134..10601
                     /locus_tag="PKW26_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HOQ92432.1"
                     /translation="MEYRALESFPYYNESKLLVEGLGYSLVELRVTPQNGRHQVRAVI
                     TRLRSEDTQSIGIDDCAKVHRLLLPRLEALIQSPEVYIEVTSPGLERLIKNAAEFSLF
                     IGKQLRVWDVEISDWLLGIIRSANEKELELELRDGNQKKIPFENITKAKLFQS"
     gene            10612..12111
                     /gene="nusA"
                     /locus_tag="PKW26_02315"
     CDS             10612..12111
                     /gene="nusA"
                     /locus_tag="PKW26_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOQ92433.1"
                     /translation="MSSQMADAIRQLTQDKGLSKEAVLQTIEDMLKAAYKRTFGTNVN
                     AIVKFNEDQSDVAIYSRKVIVDGVYDPVTEIELEEALELSEDCEVGDEIDILVDPREF
                     DRSAIQTGKQTAQQSFSEIQKDSLYAEYKDKVGEIIIGYYQRERNGTIYVDLGKVEGV
                     LPAKNQSPREVYKKNDRIRAIITEIRKSYNGLQIILSRTDPDFIRAILDLEVPEVYDK
                     IVEVHKIVREPGYRTKIAVYSHRDDVDPVGACVGLKGVRIQAVIRELEGEKIDVLKYD
                     ADPRVFIKNALSPAEVTTVIILDEEKKQALAVVPESQFSLAIGKQGLNVRLANRLADW
                     SIDVKTEDQCEEYDDLLAESRRQAQELFSDVEEYDEISNISELPQVDEDIVAILAKHS
                     IVSIHDFLEMQEAGTLPDDDELTEEKLTALNALIDSLEYDDWEEDEDFESEESDFDET
                     DDEEIEEREADFHDDEEYECPECGGVIKIDMANCPHCGVGLSFEYEDEE"
     gene            12118..>12656
                     /locus_tag="PKW26_02320"
     CDS             12118..>12656
                     /locus_tag="PKW26_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92434.1"
                     /translation="MAEEVEKKPAFILNKNPEKAQEKTPEPKGTDSSSEATKKAPSRR
                     KAVVKVVTQEKPEKKAKADTPEVKAQVEKKDTESKKESGSKKEVEHKVPSPIEGPVIV
                     RRASGSVTELSPQRPNSKQGNLADKPRVRREAGQRDGGRNYPPRREGGYRRDAGAQGG
                     QQGRDSNFNRQPGNRGPNTR"
     assembly_gap    12657..12756
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12757..14790
                     /gene="infB"
                     /locus_tag="PKW26_02325"
     CDS             <12757..14790
                     /gene="infB"
                     /locus_tag="PKW26_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOQ92435.1"
                     /translation="AGGRPQGGPRFGTGFKPGFGAAPAPMEENKKVPAKKTFKAKRPV
                     YNRRDKEENISDKLFGQKKKQTVKTNPIPESIEIMETISVSDLAKKMNLKASELIAKL
                     MSMGMMVSITQSIDSDTATLLASEFGCEVHLVSLYDETVIASQEDKPEDMASRPPVVT
                     IMGHVDHGKTKTLDAIRSANVIATEFGGITQHIGAYMVETEKGRITFLDTPGHEAFTM
                     MRARGAKVTDIVILVVAADDGVMPQTIEAINHAKDAKVPIVVAVNKVDKPEANPDKVK
                     TKLSELGLMPEEWGGDTQFVHISALKKEGLNELLDAVLLQAEILELSANHACRAEGKV
                     IESRIDHGRGVVATIIVERGTLRTGDPYVAGVYAGRVRAIFNDLGQKIDEATPSMPVE
                     ILGLEAMPNAGDPFQVTDSEKTARAISSKRQELKRFEDAKSVKKVTLDNLYETIDAGE
                     CLELKVIIKSDVQGSAEALKQSLEKLSTRDVRLNVIHASAGAINESDVMLAATDSNAL
                     IIGFNVRPTPKARVLAETEKVDIRKYNVIYKAVEEITLAMEGMLKPDVKEEIIGMLEV
                     RETFKVPKIGLIAGSYVLSGTIKRTSSVNIIRDGIVIHSGKVTSLKRFKDDAKEVNAG
                     YECGVGVDTWQDIKVGDQFEVFEYVEVARKLGESLESIKAAEAKKAAKAESKE"
     gene            14793..15194
                     /gene="rbfA"
                     /locus_tag="PKW26_02330"
     CDS             14793..15194
                     /gene="rbfA"
                     /locus_tag="PKW26_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOQ92436.1"
                     /translation="MGDFRLIRLGGQIRDEISKMIAKQTIKDPRVSTFLSINLVELSG
                     DLGFAKVYVSSFMDDAQTEKGVKGLQSAAGFIQTQLSKKLRLRQFPKLEFVRDKSIQE
                     GFDMVQKLTALEESEKQNAREDGENLDNDDE"
     gene            15187..16161
                     /gene="truB"
                     /locus_tag="PKW26_02335"
     CDS             15187..16161
                     /gene="truB"
                     /locus_tag="PKW26_02335"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOQ92437.1"
                     /translation="MNETKTKEPGLLLYAKRSGPTSFNALWDIKKALDTKKIGHTGTL
                     DSFAEGLLVVLSNGATKLVSHVTNFDKKYEAIIAFGQETDTLDPLGTIISESDFPSKE
                     ALEKTLENFTGKIEQRPPIYSSIYVQGKRASDLARDGEEVALKKRLVEVYSLELLEVD
                     NPDAIRFAHIRAHVSKGTYIRSLARDIAKSMGLCAHLARLRRTSVGPFSLEDAVFFEQ
                     LSPFNLSQLQTPVATIKTNLEKLPWQNIKDACLALGETEAKAMGFSTRILKKIFEKNF
                     SKGMNLEENWFSSPIPEDESLIFSQENQFLGAIKNINQKLSYSFVIKT"
     gene            16174..17028
                     /locus_tag="PKW26_02340"
     CDS             16174..17028
                     /locus_tag="PKW26_02340"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92438.1"
                     /translation="MKILYWKNIEKAYRKRHKTRVSKKIQEIRDFFNKPTALSIGAFD
                     GPHLGHERLFDTVLAEKKDGSLAGLVSFIQSPKMRSKKNFQGEISSLRLRTSFFEEKN
                     FDFIILIDFSPKFSRMEGKLFLSILFFVSNTKFLAIGPDFRCGFQGRTGLKEIASLVK
                     EKGIHLEVLDEFLVSGLGARSTKIREAVYVGDFETAQILLGRAYIFDLVSARKGKKIG
                     LKKNVWQISAGKTSQVLPPEGSYRVKALFADGSFLFASCIREAHFLRLELHEDSTENV
                     VAISFGYR"
     gene            17054..17323
                     /gene="rpsO"
                     /locus_tag="PKW26_02345"
     CDS             17054..17323
                     /gene="rpsO"
                     /locus_tag="PKW26_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOQ92439.1"
                     /translation="MALTKEKTAATVAAFGASAKDTGNTKVQVALLTERIKQLTEHCK
                     TFKKDKSASRGLIKLVGQRRNLLKYLERTELDTYRALIKELGLRK"
     gene            17440..19557
                     /gene="pnp"
                     /locus_tag="PKW26_02350"
     CDS             17440..19557
                     /gene="pnp"
                     /locus_tag="PKW26_02350"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOQ92440.1"
                     /translation="MNEIKRVSYKIGDEELILETGLIAKQTNGTVYAQFGGSAIIATV
                     CASGEAKEGLDYVPLTVDYNEKYYAAGKIPGGFIKREGRPKDKEILVSRLIDRPMRPL
                     FDIAFGRDIQIVPTCVSTDQVNPPDILSVIASSAAVHISDIPFNGPIAAVRVGYIDGE
                     YILNPTYEEIEKGDLEIVVAGTKDGFTMVEGGANEVSEELMLGALAKAEEFIKVMCKL
                     QDDLRELVGKEKLPLAPLEVALENKEAMLSEAYAKMETACFVKGKMERREAISKVKAA
                     LKEKYAEQLEDETQAKLFASLFEEMEYKILRKSILDKSLRVDGRSPTDIRHISCEIDL
                     LPRPHGSALFTRGETQALVVTTLGTSLDEQVYDDIDGDRSENFILHYNFPPYSVGEVG
                     RMGTGRREIGHGNLARRSLEAMMPSREEFPYTVRVVSEIMESNGSSSMASVCGGTLSM
                     LAAGVPMKKPVAGIAMGLITEGSADNPYERYAVLSDILGEEDHLGDMDFKVSGTEDGI
                     TGFQMDIKIAGVTTEIMTKALSQAKDARLHILNIMNKTISKPASSISKYAPKIESMKI
                     DVDKIGALIGPGGKTVKGISALYGVTINTDDDGTVTIYGKTAAATEDAKRAVKGIVED
                     PEVGFIYNGTVKRIMDFGAFVEILPGKEGLCHISKFSRSKINAVSDVVKEGQQIPVKL
                     LEIDKMGRLNLSYIDAIEEQEKK"
     gene            19560..20843
                     /locus_tag="PKW26_02355"
     CDS             19560..20843
                     /locus_tag="PKW26_02355"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOQ92441.1"
                     /translation="MPIALLHLDNGIDLILEPIVTAKTAAIGFWFSVGSRYEAKNQRG
                     ITHFVEHMLFKGTSSRTARDIALAFDRIGGYINAFTDRENLCVHCVVPSEYAEKAIEI
                     MNDMMNHSLFSPEEIEKERQVIESEIISSYDDPEEAANEAVFQAIWPNQSLSQSIAGS
                     LEDIASLVREDLLDWYTRYIQNGKLLICISGNFSEKKIRAALETNPDRKKVFNEPPFL
                     LPKPHEGFHFLSASFQMEQLFFCYNLEKPKTLQEYLHWHILNAIIGDTMSSRLFQSLR
                     ERAAYCYTVYSYYATSSDSALLCAYASASKKNVKKLIADMKKEMQGLFALNISEEEVE
                     ASKQHLCGEEIIASEDPEQRMKQLALQYFLDLPLVNSEERIEEIKKTKVDQIEALIKN
                     NLHVSKSAFALYGPSLGFYTKRMLKKNYKDDYAKK"
     gene            20830..21279
                     /gene="dut"
                     /locus_tag="PKW26_02360"
     CDS             20830..21279
                     /gene="dut"
                     /locus_tag="PKW26_02360"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOQ92442.1"
                     /translation="MLKNKLRILYKKNPHLNIPEYQTQGAAGADISAFLEDAITILPG
                     DFQAIPTGLFLEIPVGFEVQIRPRSGLAFKFGVTVLNSPGTIDSDYRGEVKVILINHG
                     KEAFVVNNGDRIAQMLVAPVVQAEFEEMKSLSETQRSGGGFGSTGIA"
     gene            21257..22414
                     /locus_tag="PKW26_02365"
     CDS             21257..22414
                     /locus_tag="PKW26_02365"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOQ92443.1"
                     /translation="MAQLESHNKNKASFYFSKEITKYLLKELLLFFLLSFLFFFFVFF
                     VNQILLMAQDILKKRAPLADVIKLIVYSLPFVIAQAAPFATLVGFLMCLGKLVSDNEI
                     LVIRASGFSFTALAIPVFSLGLIISIFSFAVNDYLLPMGSIAYNSLYNEIIYANPAVV
                     IEPNTIKRANDAVLIVGDLEDSVISDLLIIDKDSDGYTRIISSGKTDLLKPSSKSILI
                     KLQMSNPEIFSFDTNNLKTYNYAYGSKAILHFFSDRFFSQSSTINPREMTSYDLRKEL
                     KKIQEKNKGKRNFELTVYEIEYYKKFSLPFGAFFFSLLALPLALLFGKHNGQTIGLIV
                     GVLLSVLYWALLITGQTYGAQTNTNGFVVMWLPNAIMGIMAFILFFRLVRK"
     gene            22429..23475
                     /locus_tag="PKW26_02370"
     CDS             22429..23475
                     /locus_tag="PKW26_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOQ92444.1"
                     /translation="MAKKFFPIFLSSLAFFSFVLILLDLLINIWSYITQAVPVSSILY
                     VSYLYIPKTISFAIPLAILFAASFSLSDLSAKNELFAIFASGISLIRFTLPLLIFSFL
                     ASFGFLFFEDRIVVPTYNKKIVTQDFLLQNVESKNNSQVVILLDKGKIVYKADYYDDD
                     AQKLFGLLVVLRNDAMEVEKILRASSASWREDQWLYSGETIFEIINHEVLVTKDISGL
                     EIREKPEVFRSNVINIEAENIQNSKLYLEHLEKTGLPMAESLSLYYKKFSFPFIVFIV
                     VFLSIGVSGKTKKNVLLVSLASSLGSAVLFYVTQMLTMLMAKFSVISPLMGAWFPVFL
                     FVGISLVLLRYSKT"
     gene            23521..24657
                     /gene="tgt"
                     /locus_tag="PKW26_02375"
     CDS             23521..24657
                     /gene="tgt"
                     /locus_tag="PKW26_02375"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOQ92445.1"
                     /translation="MKEIFTIEHQDENSRARTGRLVLPHGSVQTPVFMPVGTNATVKA
                     ITVDDLHEIGFEIILSNTYHLYLRPGSDVIGNLGGLHGFSRWDKNILTDSGGFQVFSL
                     APFRKIREEGVEFKSHIDGSSHFLTPEKIVQVQRQLNSDIQMQLDVCSSWGVDYAEAK
                     ESLRITNDWLLRAKKEWKKTQADGYQGLLFPIVQGNFFKDLREESADFVSNLDMPGIA
                     IGGLSVGEPLEIYQEMLSFTAPLLPYEKVKYVMGIGTPDYILDAIENGIDMFDCVLPT
                     RNARNGSYFTRDGALAIKKEEHRLSAEAIDAECNCKVCKNYSRAYLRHLFKTNEMLSS
                     MLASYHNLYFLHDMVKNAREAIRKNNFTAYKKCFLDRYFKRGAK"
     gene            24654..26000
                     /locus_tag="PKW26_02380"
     CDS             24654..26000
                     /locus_tag="PKW26_02380"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOQ92446.1"
                     /translation="MKKCHGFCIVFFFLFSFFIFSAGADFTEIEKRERTLVYGLESDV
                     TSLIDTLIKEKDFTYTNQLFDIYVQSQNIVVREKIILYFMNAEDYALKELVLETLEDP
                     WDEKKQVVKAMLQYVGKLKITEAGPFILDLVKTDDEAYFENAVIALGLVGGEEEALYL
                     AEYFQNEAPSIIKKQALMKALVNMNEEAIWDFLVETMQNEDENTYVRMYASEALGNTK
                     KPEAVDLLIDMFSNSDINIRVYAIKGLANFNDEKTIQVLIEALKDNNYRVRLEAIEAF
                     KKNKDERAADSLLYRAEHDPEMSVKQKSYETLAILNTEAVQRFFKKAIENAKHNTDLR
                     IKAAAVSLEQEIEAVYQSIFDLAKESLKDDKLKNIRYALGKEFAKYKNKAFEEVCELY
                     LSHTDVSTKGTGLDIFITNAYVSLVPKVQAIANNDKEGANQKKARLALEKTGFDLK"
     gene            complement(26002..>26340)
                     /locus_tag="PKW26_02385"
     CDS             complement(26002..>26340)
                     /locus_tag="PKW26_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOQ92447.1"
                     /translation="SRNIHALSAGARKSIGGGLSVFSIFFIKVPANIAEIRYDGQSCS
                     LALLSPKYFPEADSNIIEDCLNTPITIISDHGYITRIMFSEYESQTEKLNELLLSVIP
                     EDDKKKYIKK"
BASE COUNT         8211 a         4587 c         5238 g         8104 t
ORIGIN      
        1 cttcaaaaag tattttttta caaagtcaaa atttttctgg tggcttgcat tatcctctaa
       61 tgaaagactc tgcggtaatt tttgtttcaa gtttttttga tcacgattct ttgtttgtct
      121 caccctttga aaccattttt aaagatgctc gctttatcaa gtctgaaaaa acattcgacg
      181 atgcggatgt aatacttgaa gaagtccttg ccgagggttt tgaaagagaa tccatggaag
      241 ctattgcgtc catagaatcg gtagatgacg aggcaagacc agaaacaact tcccttgatg
      301 cgtataaaac aaaaaaatat aatcctatcg catatatgac gaaaggaatt tttcttccca
      361 cactatcgct tgagataatt gatgatacaa gtgataacga cgaatttgat ctttcattgt
      421 ctttcggttt tatgtactta accggagatc ctgctgatac atggcagatt ctcattcagc
      481 cttcttacga ttttaccaat gaaaaaatat acttacaaac agaaattgca acaacagctt
      541 ttcctttacc tcttagtctc gagggaatct cagtttttgg tttagatggc tctgttcaaa
      601 acgattacac tctttcatcg gctatagaaa ttcccctagg aaattcgccc tatagcttta
      661 acttttccac agctcttttt ttatcgcatg gcaaaaaaaa tgcagaatcg gcttttttca
      721 attttctgca taacggaaat attctttctt tttcatctat tcgaaatgca aacttaaatc
      781 cttttgcctt gaaaggtttt gagctaggag cttctctttg gttttttgac accaaggatc
      841 cagagttttc tcttgatacg aactacgcat ataatttttt tgcgtcttat tgtctaccac
      901 aagttttgcc aattcaaaat ccgattggct ttacctataa tctaccgaca gtttttgaag
      961 caagtctttt tacttcacaa aatgtatatg aagaagagga agcgctttgg tctgcaagtg
     1021 ctacaacaac cttgtttgcc tttgatttcc aaaatgccct ttcaaatatc tatatacaaa
     1081 gatttatttt tgaagcagag tatgcatgta tgcaaggatt ggattatatt gattataaaa
     1141 atcccctaga taatttttca catattgaaa gaaatttatt taaagactct gttgccattt
     1201 ctgcacattt tacttcttca gtgatagtcg gaatgttact cacacaatta aaaatagatt
     1261 taggtgctac ctttcgttat catattaaag atagaaaacc tgaatggtcc ttgcttatac
     1321 gttttcattg atggcggtta taataataca attttattat tgaaaacact gtatttctat
     1381 gatagtgtaa gagaatgaaa tctataaatg caccatccga tataaatgtc tctcgcattt
     1441 tacgccttgt ttggcaaaaa agagaaatta gtcgtataga aattgcctcg caacttggga
     1501 ttgataaatc aactgttaca aaaattgttt cttcacttat agatatgggg ttaatttcag
     1561 aactttctga aggaaatacc ggtccgcaag gaggtcgaaa acctattttt ctagaaattt
     1621 ctccttcgtt tgcttgcgtc ggaggagtag aaatcaattc aggacgctat gtttgttgcc
     1681 ttctcgatat gtatggaaaa gttttgtttc aatatgctga agcgatagaa ccaggatcgt
     1741 atgaaataga tatgtatcag ggcatttttt tgaaagcctt tgagacactt tcttctgagg
     1801 cagaaaaaaa aggtttaccg cttgttggga taggggttgg ttttccaggt atagtaaatt
     1861 cagaagaggg tattattact caatcagtac ctctgatgat agacgaagcc tatgcttttg
     1921 ttgaagaagt ttctaaggaa atagctattc ctctttttat tgaaaatgat gcacgctgtt
     1981 gttgctatgg cgaaatgctt ttggcaaaaa cacatgacgt taaaaatatg ctctatgttt
     2041 tagcagaata tcgtatttta caaccaacaa agtcttcgca aaaaaatctt tcagtcggtc
     2101 ttggtattgt ttttgatggg cttatattga aaggggccga tttttcggca ggagaatttc
     2161 gtagcctttt atgggatgaa gggaataagg ggcagtttta ttccggtgaa aaaaaacttg
     2221 aacaacttga tgaaggtgct gtgcaaccgg ttttttttga acttgcccag catattgctt
     2281 ttttagtaaa taccttaaat ataaatcaag tatatatcgg tggaatcgat aaaaaatatg
     2341 ctgaacaact catagatagc ataaatactc gtatacgctt tcaatggccc tataaagcga
     2401 agcaaaacta taaggtttcc ttagcatcgt tagaaatgtt tgctgtttct tatggagcgg
     2461 ctgcaatgtt tttggaacaa atatttgctt tgccgaatct ttccttaaaa tcaggcatgg
     2521 gagaatcagt tttgaaaaca tttgaaagct taaaaaagaa ctaagcccct aaaatctttt
     2581 ttttatccaa aaattacaaa aaaaaagcaa aaaaatgaaa gatttcgctt tacagatttc
     2641 attttcaatg ttactattat gttagttgaa ggaagcaatt aactaagtag ctctatcacg
     2701 taagtgattt aagcatattt cattactctc aaagggggaa aaaatgagac tttctaagaa
     2761 agtgttgctc gtgagtctag ttctcgtact cgcagcagga atggtttttg caggtggttc
     2821 aaaagacaca gcaaaaaaac aaggtaaggt tttaacaatt tattgttgga atgatgaatt
     2881 tcaaactcga ttcaatgact attttgcagc aaaaggttta gtgccagatg gtattgaagt
     2941 gaattgggtt attacaccaa atcaaggaaa tgcctatcaa gacaagctcg atgaagcttt
     3001 gttgcgacaa aattcagcat cagagcgcga tattattgac attttccttg ttgaagcaga
     3061 ttacgcacta aaatatgtcg atacaccgtt tacactcgac gtatttaaag atgttggttt
     3121 gacaagtgca gatgttgcaa atcagtataa atatacaaaa gatattatga ctgattcaaa
     3181 gggtgttctt aaaggcgttt cttggcaagc atgtccaggt ggattcattt accgacgttc
     3241 aattgctaaa gctgttcttg gaactgatga ccctgcaaaa gtgcaagagg ctttgagttc
     3301 ttgggataaa tttgatgcag ttgcagcaca agcaaaggca aaaggctatt ttatgctttc
     3361 tggttttgat gatgccttcc gcgttttctc agacaacgct aaaactcctt gggttggtgc
     3421 aggcgacaaa attcaaattg atgcaaatat tgagcgatgg atagaacaaa ctaagactta
     3481 tactgataaa ggctataaca ataaggcaaa ccttttttca gctgaatcat ggggtggtgg
     3541 tatgaaagac ggtaaggtat ttggttactt tggtccttca tggttccaag atttctgttt
     3601 agctcctgct gttgataccg ctggtgactg ggctgtctgt actggtcctc aaggtttctc
     3661 atggggtgga acatggattt gtgcagctgc tggaactgac aatattgacc tcgtaaaaca
     3721 agtaatgctc actcttacta cagataaaga tacccttgtt gctatcgcac aagaagcagg
     3781 tgactttact aaccatgaag gcgctatggc tgaaatcgca aaaagtagtt atggaaatcc
     3841 attcttaggt ggacaaaacc acattgccta tctgcttgag tctgcaaaga aaattgacag
     3901 aagtagcatg ggcccttatg accaaggcat gacagaaaca attcaggctt catttgctga
     3961 ctatttcaat ggttctgtta cgaaaggaca agcatgggac aacttctata aagctataat
     4021 cgaaatatac ccaaatctta agaagtagtt tttaattact tgaaaaaaca agcaaggcag
     4081 cttttgcctt tgcttgtttt ttttgcatat acaggaggaa agtgatgaca aaaacaagta
     4141 gaccgcaaaa aaaacgactg gtcgattatt ctaagtgggg ttattttttt attgcaccat
     4201 tttttattat ttacgtgata ttttctctca tacccttgct atctactttt gtatatagtt
     4261 tttttgaaag ctatcgcatc ggtttgcagc aagttggacc aaactttgtt ggctttgcaa
     4321 attataaagc ggtactggaa agcgattttt taaaatatac aatgaacaca ttgcttttat
     4381 ggatactcaa ttttgtgcca caaatactgg tttccttagt tttagctgta tggtttacca
     4441 gttcgagttt acgactaaag ggacagcctt tttttaaaac aattatttat atgcctaact
     4501 tgattatggc atcagccttc gctatgttat tttttgccct cttttcagat aagggaccgg
     4561 taaatattat tctcgaaggt atggatatag aacctattcg atttttaacc aagatttctt
     4621 ggactcgtgc gcttatttca attatgaatt ttttaatgtg gtttggaaat acaaccattt
     4681 tactcatggc aggaattttg ggcatagatg aaagtttgtt tgaagcctca cgtatagatg
     4741 gagcgagcgc tttacaaaca ttttttaaaa tcacgatgcc gcttttaatg cccattttta
     4801 tatacgtact aattacctca atgattggtg ggattcaaat gtttgacgta ccacaaattt
     4861 taacgaatgg aagtggaaat cctaaccgaa caagtatgac cttaattatg tacttaaatc
     4921 gacatttaac aagtaggaac tttggtctcg gtggcgcagt ttctgctttt attttcctta
     4981 tgacatctgt gcttggtttt tttacctatc gttctttgat gtcgcaatat agaaaatcac
     5041 cttcaagaaa aagttaagga gtacaattta aatgactaca gaaactacta tatatagaaa
     5101 accaaaagga aatgtttttc tgcaattgca acgccgctta gcttacttgt ttttattgtt
     5161 tcttgctgcg atttgtattt ttccttttct tattttactt gtaaatgcat cgcgatcgaa
     5221 tgcacaaatt caagttggct ttacactctt gccgggaaaa ttctttgtaa caaacttttt
     5281 aaatcttgta caagacccaa acatgactat tcttcgctct cttttcaatt cggtttttgt
     5341 ttcaagtgca actgcttttt tctcaaccta tttttctgcg atgacagcat ttggtattta
     5401 catgtatcgc tttaagggta gaaattttgc tttcaatttt ataatgcttg ttatgatggt
     5461 gccagggcaa gtttcagctc taggttttat tcaaatggtt acaaagatgg gcttatacga
     5521 tactttttta ccgcttatca taccagctat tgcttctcct gttgtgtttt tttacatgat
     5581 gcaatacatg gagtcggtgt taccttttga aattgtcgag tctgcaagaa tagatggagc
     5641 aaatgaattt tttaccttta atacaattgt tctacctatc ttaaaacctg ctattgctct
     5701 acaggcaata tttagctttg ttggaagttg gaataattat tttatgcctg ctttgatttt
     5761 aagttcaaaa aataaaaaaa caatccctat cttaattgcg caattgagaa gtgctgatta
     5821 tatgaagttt gatttgggca aggtgtatat gcttatttgc cttgcgatta ttccgctgtt
     5881 aattgtgtat ttaattcttt ctaaacaaat tatacgcgga gttactatgg gaagtgtaaa
     5941 aggttaagtg cctcctaaaa atacctttgt tcaagcatgt agtagacttg tttccctgca
     6001 attttattac atgcttggac ttttttttac ataacaaaac ggtttttcat ttttgctaga
     6061 aaatcaattg cctcagcatc ggaaactgcg ccctttggtt catgatagct tacaagattt
     6121 tctggaattt catctagaaa aggagacggc gtgcattcaa ctacggtttg catttttctt
     6181 cgccttctac acgagctaat aaataattta tcttgagccc gtgttatagc aacgtaaaaa
     6241 agccgccgct cttcttctat atttccatct ccctcttcca cacttcgagc gtgtggaatg
     6301 aggccgtctt ctgcaccagc tataaaaaca accggaaact ccaatccctt tgaggcatga
     6361 attgtcataa gatttacttt tcccaacaag ccctcttcat cttccatgtc atcgcgcgag
     6421 agaagggtaa tgcgattaag ataggcataa agacctgagt caaaggtgtc ggggttattt
     6481 tcccagctat ctatacctga gagtaaatgt tcgatattta aaaacttaaa acgcgcggcc
     6541 ttttcatttt tttgatattc agccatgaga tagtccatgt agtttatttc atcgaccata
     6601 gcacgcactt tttttgacaa accctttcca ccgctcaaaa gaagacttct ttgttttccg
     6661 atgagctcta caaattctac caaatcagcc tttgctctat cgctcaaagg gctttccgat
     6721 gcggcaatca atgcattgat acttgtccat agtgagcatt tttggcttgt tgatattgcc
     6781 gatatttgct cgatggtttt tttcccaatt cctcgccgag gtgtattgat aatgcgcaaa
     6841 agatttatat catcatcgtg gtttgatata agccgtagat acgaaataat atcttttatt
     6901 tcctttcttt gaaaaaaact cgttccacca gatatgctat gtggaatatt ttctgccaaa
     6961 aaagcttcct ctatcgcacg agtcattgta ttcgctcgta tgagaacacc aaagtcccca
     7021 aactttcgcc cctcgctcat tcgcaagctt tggatcatct cggcgataaa gcttgcctca
     7081 tcatgctcgt cctcaggcat atatacttct atgggttttc cactgccatt tcccgaccac
     7141 aagcttttat ccttgcgatt tgtattgtgc gagataactc cgtttgccgc agctaaaata
     7201 gtttctgttg atcgataatt ttgttcaagt cgtatttcca ttacatttgg aaagtctctt
     7261 tcaaacataa gaatgttttc atannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnagctgct tgtatgcgtc attcgatgcc tcccaagtcc agcgccctat tttaatattt
     7441 gaaaaaagat ttgctatctt gtatatatcg agtccctcgg ttttaagccc tagttctcga
     7501 gctgtttctt ttataagttg gtttttatcg acctcatcat aaatagaaaa attttctctc
     7561 caaccgaggg tctctatgtc ttgcctaagg atttttaccc caaaggcatg aaaggttgat
     7621 atgcttaaat tttgcagttt ttttcctgta agctcacgta ttctctcttg catttcttta
     7681 gcagccttat ttgtaaaagt gagcgccaaa atctgacttt gtggaatacc catctcaagc
     7741 atgtggacaa tgcggtttgt aataacccgc gttttaccac tccctgcacc cgcaataatg
     7801 agcaaggcac cctcaattgt tttaactgcc ttgtcttgtt gttcatttaa tctcatagtt
     7861 gacatagaca attatcataa tcagaaaagc ctgttgatac aaggggacaa tggattttgc
     7921 aaaactcgcc caagagctaa tagtgaggac aggtacgtta ctttttgcat ccatgcaaaa
     7981 agtaactttg acagttttgt ttgcaaaact gtcgtagttt tttcaaacat ggatgtttga
     8041 aaaaacgttt gtttcttctt gaatttttct ttttgcaatg ttatactttt tagacatttt
     8101 atattgacta ttttggagga tataatgaca gttgcacaaa aggtaaatgc ttttctgggt
     8161 aaaaatggtt ttacttgtgg tggcccaaat ataaatgcgg taattgatag ccttatttgg
     8221 gatatgaaaa aaggcttaaa tgaaggacga tctacagaag gcgcatttgt agcgtcacaa
     8281 gatatgatac caacctttgc gatgcctcca accgagcgta tcgtcaataa aaatgtaatt
     8341 gttattgatg ccgggggaac gaattttagg gcttgtttgg ttcattttga tgcagaaggt
     8401 acttatacca tatctgattt taaaaagacc gcgatgcctg gtattgaacg ggaatatgga
     8461 aaaaaagaat tttacgatac gattgcaaaa aacctcgatc acttaaaggg caaggcagat
     8521 tctatcggtt tttgtttttc ttacgcaatg agtattacgc cggaaggaga tggacgtgtt
     8581 cttgatttct caaaagaaat aaaagcaaaa gaggttgtag gaagtcttgt cggagaatct
     8641 ttgagtgcag ctcttgtaga aagaggatgg gaaaaaccga agaaaattat tcttttaaac
     8701 gataccgccg cggctcttct ctccggggca gcaagtgcaa aacccggtcg tctttttagt
     8761 tcctatgttg ggctcattct ggggaccgga atgaatgccg cttatattga aagcaatcca
     8821 ataaaaaaaa ttgcagggca aaaagctcct gaaagccaaa tcgtcgtttg tgaaaccggt
     8881 aagtttgata aattgccgca atcacttttt gatatagcct atgataaaac aactaatact
     8941 cctggaatgt atataattga aaaaatgtgt tcaggagcat atttaggacc agtaacagga
     9001 tttgctttaa aacaagcggc aaaggatggt ttgtttagcg aagctgttgc aaaagacctt
     9061 cttttaatcg aaaaatttga attaaaagat atggatcaat tcttttttgc tccttacaac
     9121 acagatactt tgcttggtgc aattatggca aagggcaatc aagatgatta tgatacgtgt
     9181 tatgctctcc tcgatgcctt tgtcgaacga acagctcggc tcgcagcatc tattatagca
     9241 gcctgcgtaa taaaaagtga aaaaggtact caagcatccc tccctgtctg tgttgttaca
     9301 gaagggacga cgtattttaa aacgcataac ttacaagatc gtgtaaaagg ctatcttaac
     9361 acagtgctga tggaagaacg aggtttatct tttgagttag taagccctga gtctgcaatt
     9421 accctcggtg ctgctgttgc ggctttgtca taatatatat acaaaatact gagaaaacgc
     9481 ttttagtttt aaaagtgttt tctcttaatg tgctgcttct cgaatttctt ttatttcttt
     9541 atttaagaag agagaaacaa ccgttcgtat tgtaataatt gcagcgagga gtatgatatc
     9601 atcgattgtt gggttgatga tggtttcgat tatgtctgca acaataagta cttctaagcc
     9661 aagtaggaca taactaccaa gctcggtttt catgtactgt agacgagaaa ttttttcaga
     9721 ttttggtatt tttgctataa ggatgaggaa aaggtctcgt gcgcaaaaaa caacaccaat
     9781 acataaaata ataacagata agacgttgat accgagtttt accattttta ttatcggaag
     9841 aattgtttgc attttatctc cttgtatacc tttatgattt cgcataaatg atttcacttt
     9901 tagtatacac tgccttttct tattttacaa ttttttttcg cttagattta agctgattca
     9961 atgcgcagta ttgacaagat tttagccact tgctatactt actatagaac ataagttaat
    10021 atcctttact ttcaggagtc gttcaaacga ctcttttttt gtatcttata gccaagcatt
    10081 tttttgaatt caaaacaatt gctttcgttt acaaattttt tataggagta tttgtggagt
    10141 atagagctct agaatcgttt ccttattata atgaatcaaa actcttagtt gaaggcttag
    10201 gctattctct cgttgagctt cgtgttactc cacaaaatgg gcgtcatcaa gtacgagctg
    10261 ttatcactcg tcttcgttct gaagatacac agagcatcgg tatcgatgat tgtgcaaagg
    10321 tgcatcgtct ccttttaccg cgacttgaag cacttataca aagtcccgag gtatatatcg
    10381 aagtaacttc tcctggttta gagcgcttga taaaaaatgc agcggagttt tctttgttta
    10441 tcggaaaaca gcttcgtgtt tgggatgtag agataagtga ttggcttctt ggcattattc
    10501 gcagtgcaaa tgaaaaggaa ttagaattgg aactacggga cggaaatcaa aaaaagatac
    10561 cgtttgaaaa tattacaaaa gcaaaattat ttcaatctta gggggcttga gatgtcatcg
    10621 caaatggcag acgctatccg acaattaaca caagataagg gcttaagtaa agaagccgtt
    10681 cttcaaacaa tagaagatat gctcaaggca gcatataaga gaacttttgg gacaaatgta
    10741 aatgcaatag tgaaattcaa cgaggaccaa tcagatgtcg ctatatattc tcgaaaggta
    10801 atagttgatg gtgtttacga tccagtaact gaaatagaac ttgaagaagc attggagctt
    10861 agtgaagact gcgaagtagg cgatgaaatc gatattcttg tcgatccgcg tgagtttgat
    10921 cgttctgcaa ttcaaacagg aaagcaaaca gcccagcaat cttttagtga aattcaaaaa
    10981 gatagcttat acgctgaata caaagataag gttggagaaa ttattattgg ttattatcaa
    11041 cgggagcgaa atggaacaat ctacgttgat ttgggtaagg ttgaaggtgt tttacctgcc
    11101 aaaaaccaat ctccacgaga agtttataaa aaaaatgata gaatccgggc tataatcaca
    11161 gaaatacgta aatcctataa tggcctacag attatactct cacgaactga tcctgatttt
    11221 atccgtgcaa tattggattt agaagtacca gaagtatatg ataaaattgt tgaagtacat
    11281 aaaattgtac gtgaaccagg ctaccgaaca aaaattgcgg tttattctca tcgagatgat
    11341 gttgacccag ttggagcttg cgttggttta aaaggtgtgc gcattcaagc tgttattcgt
    11401 gagcttgagg gcgaaaaaat tgacgtactt aagtatgacg ctgacccaag ggtttttatc
    11461 aagaacgccc tctctccagc agaggttact actgttataa tcttggacga agagaaaaaa
    11521 caagcccttg cggtagtacc tgaaagccaa ttttctttag caatcggtaa acaagggctg
    11581 aatgtccgct tggcaaatcg tcttgctgat tggagtatcg atgtaaaaac agaagatcaa
    11641 tgtgaagaat acgatgattt actggcagaa tcgagacggc aagcgcaaga gctctttagc
    11701 gatgtagaag aatatgatga aatatcaaac atttcagaac ttccacaggt tgatgaagat
    11761 attgtggcaa ttttggcaaa acactcaatt gtcagtattc atgacttctt ggaaatgcaa
    11821 gaagcaggta ccttacctga tgatgatgag cttactgaag aaaaattgac tgcgttgaat
    11881 gctcttattg attcattaga gtatgatgac tgggaagaag atgaagattt cgaatctgaa
    11941 gaatctgact ttgatgagac agatgatgaa gaaatcgaag aacgtgaagc tgactttcac
    12001 gatgatgaag aatacgaatg ccccgaatgt ggtggcgtaa taaaaatcga tatggcaaac
    12061 tgtcctcatt gcggtgtcgg tcttagtttt gaatatgagg atgaagagta ggtgataatg
    12121 gcagaagaag tagagaaaaa accagcattt attttaaata aaaaccccga aaaagcacaa
    12181 gaaaagactc ccgaacctaa agggacagac tcatcttctg aagctacaaa aaaagcacct
    12241 tctcgaagaa aggcagttgt aaaggttgtt acacaagaaa aacctgaaaa aaaagctaag
    12301 gccgatacac ctgaagtaaa agcacaagtg gaaaaaaaag atactgagtc taagaaagag
    12361 agcggttcta aaaaagaagt ggaacataaa gtgccttctc ctattgaagg tccagttatt
    12421 gtaagacggg catctggttc tgttaccgaa ctgagtccac aacgtccaaa cagtaagcaa
    12481 ggaaatcttg ctgataagcc acgtgtgcgt cgcgaagctg ggcaacgaga tggcggcaga
    12541 aattatcctc cacgacgcga aggtggctat cgtcgtgatg cgggagcaca gggcggacag
    12601 caaggtcgcg atagcaattt taacagacaa cccggaaatc gaggtccaaa tactcgnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngctg gtggaagacc acaaggcgga
    12781 ccacgttttg ggacaggatt taaaccaggc tttggtgctg ctcctgctcc tatggaagaa
    12841 aacaaaaaag ttccagcgaa aaaaacattt aaagcaaaac gccctgttta caatagaaga
    12901 gacaaggaag aaaatattag cgataagctt tttggacaaa agaaaaagca aaccgtaaaa
    12961 acaaatccta tacccgaaag catcgagatc atggaaacga tttctgtttc agatcttgca
    13021 aaaaagatga acttaaaggc atcagaattg attgcaaaac tcatgtccat gggtatgatg
    13081 gtttctatta cgcagtctat tgactccgat acagcgacct tgcttgcctc tgagtttggc
    13141 tgtgaagtgc atttggtgag cttatacgat gaaaccgtta ttgcaagtca ggaagataag
    13201 ccagaagata tggcttctcg tccacctgtt gtaacaatca tgggacacgt tgaccacggt
    13261 aaaacaaaaa ctcttgatgc tattcgttct gcaaacgtta ttgccactga atttggtggt
    13321 atcacccaac atattggtgc ttacatggtt gaaaccgaga agggtagaat aaccttcctc
    13381 gatacaccag gacacgaagc ctttactatg atgagggcac gtggtgcaaa ggttactgac
    13441 attgttattc ttgttgtagc agccgatgac ggcgttatgc cgcaaacaat agaagctata
    13501 aatcacgcaa aagatgccaa ggttcctatt gttgttgcgg tgaataaagt cgataaacct
    13561 gaggcaaatc ccgacaaggt taaaacaaag ctttccgagc ttggtctcat gcctgaagaa
    13621 tggggtggag acacacaatt tgtacatata tcagccttaa aaaaagaagg cttaaacgaa
    13681 ctcctcgatg ctgttttatt acaggctgaa atcctcgagt taagcgcaaa tcacgcctgt
    13741 agagctgagg gtaaggttat agaatcgcgc atcgaccacg gtcgtggtgt tgttgcaacg
    13801 attatcgtcg aacgcggaac cttgcgaaca ggggacccct atgttgctgg tgtttatgca
    13861 gggcgtgttc gtgctatctt taatgacctt ggacaaaaaa tcgatgaggc aacgccgagt
    13921 atgccggttg aaatccttgg tctcgaagcg atgccaaatg caggcgatcc cttccaagtt
    13981 accgactctg aaaaaacagc ccgtgctatt tcttctaagc gccaagaact caaacgcttt
    14041 gaagatgcaa agtcggtcaa aaaggttaca ctcgacaatc tctacgaaac cattgatgcg
    14101 ggtgaatgct tagaactcaa ggttattatc aagtctgacg tgcaaggttc agctgaagct
    14161 ctcaaacagt ctcttgaaaa actttcaacg cgagatgttc gcttaaatgt tatccatgca
    14221 tcagccggtg ctataaatga aagcgatgtt atgcttgcag caaccgattc aaatgcgctt
    14281 attattggct ttaatgtgcg accaacacca aaggctcgag ttcttgcaga aactgaaaaa
    14341 gttgatattc gtaagtacaa tgttatttac aaggctgtgg aagaaattac ccttgcaatg
    14401 gaaggcatgc tcaaacccga tgttaaggaa gaaatcattg gtatgcttga agttcgtgaa
    14461 acctttaaag tgccaaaaat tggactcatc gcaggctcct atgtgctcag cggtaccatt
    14521 aaacgaacaa gcagcgtaaa tattatacgt gatggcattg ttattcactc tggcaaggtt
    14581 acttctctaa aacgatttaa agatgatgca aaagaggtca atgctggcta tgaatgcggt
    14641 gtcggtgtcg atacctggca ggatataaag gttggcgatc agtttgaggt ttttgaatat
    14701 gttgaagttg cacgaaaact cggcgaatct ttagaatcta taaaagcagc tgaggcgaaa
    14761 aaagcagcga aggctgaaag taaggaatag cgatgggaga ctttagattg atacgtcttg
    14821 gaggacaaat ccgagatgaa atttcaaaaa tgattgcaaa acaaacaatc aaagacccac
    14881 gtgtttctac ttttttgtcg attaaccttg tagagctttc gggagactta ggtttcgcaa
    14941 aggtttatgt ttcaagcttt atggacgatg cgcaaacaga aaaaggcgta aaaggtttgc
    15001 aaagtgcggc tggttttatt caaacccagc tatcgaaaaa acttcgcttg cgacaatttc
    15061 ctaagctaga attcgtgcga gataaaagta ttcaagaagg ctttgatatg gtgcaaaaac
    15121 taacagctct tgaagaaagt gaaaagcaaa acgctcgtga agatggagaa aatctcgata
    15181 acgacgatga atgaaactaa aacaaaagag ccagggcttt tactctatgc aaaacgctct
    15241 ggtcctacca gttttaatgc cttgtgggat ataaaaaaag ccttagatac aaaaaaaata
    15301 ggtcataccg gtacccttga cagttttgcc gagggtttac tcgttgtttt gagcaatggc
    15361 gctacaaaac tcgtaagcca tgtaaccaac tttgacaaaa aatacgaggc tattattgct
    15421 tttgggcaag aaaccgacac ccttgatccc ctcgggacga ttatatctga atccgatttc
    15481 cctagcaaag aagcacttga aaaaacactt gaaaacttta cagggaaaat agaacaaaga
    15541 ccacctattt attcatccat ttatgtacaa ggaaaaagag caagcgatct tgcacgggac
    15601 ggcgaggagg ttgccctaaa aaaacggctt gttgaggtat attctcttga acttctagaa
    15661 gttgacaatc cagatgctat tcgttttgct catattcgtg cccacgtttc aaagggaacc
    15721 tatattcgat cccttgcgcg agatattgca aagtcgatgg gactttgtgc gcatcttgca
    15781 cgattacgcc gcaccagtgt cggtcctttt tccctagaag atgctgtttt tttcgagcaa
    15841 ctctccccat ttaatctaag tcaattgcaa actcccgtag cgacaataaa aacaaactta
    15901 gaaaaacttc cctggcaaaa tataaaagat gcctgcctcg cgcttggaga gactgaagca
    15961 aaagcgatgg gtttttctac ccgtatctta aaaaaaatat ttgaaaagaa tttttctaag
    16021 ggtatgaact tagaagaaaa ttggttttca tctcctattc cagaggatga atctcttatt
    16081 ttttcacaag aaaatcagtt tttgggtgca ataaaaaata taaatcaaaa actcagttat
    16141 tcttttgtta taaaaacctg atttgggctt gctatgaaaa tattgtattg gaaaaacata
    16201 gaaaaagcat atcgcaagcg gcataaaaca agggtttcaa aaaaaataca agaaatacgt
    16261 gattttttta acaagcctac tgccttgagt atcggtgctt ttgacggacc gcatcttggg
    16321 catgaaaggc ttttcgatac tgttttagca gaaaaaaaag acggctctct tgcaggcctc
    16381 gtgagtttta tacaatcgcc aaagatgcga agtaaaaaaa actttcaagg tgagatttca
    16441 agcttgcgtt tacgtacttc tttttttgaa gaaaaaaact ttgattttat catactcatt
    16501 gacttttctc ccaaatttag tagaatggaa gggaagttgt tcttatcgat tctttttttt
    16561 gtaagcaata cgaagttttt agccatcggt ccagattttc gttgtggctt tcaaggaaga
    16621 acaggactga aggaaattgc ctctttggta aaagaaaaag gcatacactt ggaagtgctt
    16681 gatgaatttt tagtttcagg cttaggagcc cgatctacaa agattcgtga agcagtgtat
    16741 gtaggagatt ttgaaacagc tcaaatcctt ttgggaagag cctatatctt tgatttagta
    16801 tctgcacgaa aagggaaaaa aataggtttg aaaaaaaacg tatggcagat ttccgctgga
    16861 aagacaagcc aggttttgcc accggagggt tcatatcggg tcaaagcact ttttgctgac
    16921 ggctcatttt tgttcgcttc ctgtattcgt gaggctcatt tccttcgcct cgagttgcac
    16981 gaagacagca ctgaaaatgt ggtggcaatt agttttggat atagatagga aaaattatac
    17041 taaggagtaa gtaatggcgc ttacaaaaga aaaaacagct gcaacagttg cagcatttgg
    17101 tgcgagtgca aaagacacag gcaatacaaa ggttcaagtt gctttattga cagaaagaat
    17161 taaacaatta accgaacact gtaaaacctt taaaaaagac aagtctgcat cacgcggttt
    17221 aataaaatta gtcggacaaa gaagaaattt attaaaatat ctcgaacgca cagagcttga
    17281 tacctaccgt gcgctcatta aagagttggg attacgaaaa taagtagttt taaaccctgt
    17341 ataaacctta gttagcctca agagttaact aaggttatat tcgttttaaa tgaatagttt
    17401 cgcaaaatgc ttttctattt atttatacta ggagaaaata tgaacgaaat taaacgagtt
    17461 agttataaaa taggtgatga agagcttatt ctagaaactg gcctcatagc aaaacaaacc
    17521 aatggaacag tgtatgcaca gtttggcggg tctgcaatta ttgcgacggt ttgcgcctct
    17581 ggggaagcaa aagaaggctt ggactatgtg cctcttaccg tagattataa tgaaaaatac
    17641 tatgctgcgg ggaaaattcc tggcggtttt attaaacgag aggggagacc aaaagacaaa
    17701 gaaattttag tttcacgtct catcgaccgc ccaatgcggc ctctttttga tattgccttt
    17761 ggtcgcgata tacagattgt acctacctgc gtttcaactg accaagtaaa tccaccagat
    17821 attttatctg ttattgccag ttctgctgca gtacatattt ccgatattcc gtttaacgga
    17881 cccatcgctg cagtgcgtgt tggctatatc gacggagaat atatcttgaa tccaacctac
    17941 gaagaaattg aaaaaggtga tttggaaatt gttgtagcgg gaacaaaaga cggttttacc
    18001 atggttgaag gaggtgcaaa cgaagtctct gaagagctca tgctcggtgc acttgctaag
    18061 gccgaagaat ttatcaaagt catgtgtaaa ttgcaagatg acttgcgcga acttgttggt
    18121 aaagaaaaac tccctcttgc ccctctagaa gtagctcttg aaaataaaga agcgatgctt
    18181 tctgaagcct atgcaaaaat ggaaacagct tgttttgtta aaggtaaaat ggaaagaaga
    18241 gaagcgattt caaaagtgaa agcagcttta aaagaaaaat atgcagagca acttgaagat
    18301 gaaacacaag caaaactttt tgcatctctt tttgaagaaa tggaatacaa gattttacgt
    18361 aagagtatct tagataaatc tcttcgcgtt gatggtagaa gtccaacaga tattcgccat
    18421 atttcttgtg aaatagatct ccttcctcgc ccacacggct cagctctttt tactcgtggc
    18481 gaaacacaag cgctcgttgt tacaaccctg ggcacgagtt tagacgaaca ggtctatgac
    18541 gacatagacg gagatcgaag tgaaaacttt attttacact ataacttccc tccctattct
    18601 gtgggagaag tgggacgcat gggaacagga cgccgtgaaa taggtcacgg gaatcttgcc
    18661 cgacgctcgc tcgaagcaat gatgccaagt cgagaagagt ttccctacac tgttcgtgtt
    18721 gtctctgaga ttatggaatc aaatggttcc tcatccatgg cctctgtttg cggtggaaca
    18781 ctttctatgc tcgctgctgg tgtaccgatg aaaaaaccag ttgcaggtat tgctatgggg
    18841 cttattaccg agggcagcgc tgacaatccc tatgaacgct atgctgtttt atcagatatt
    18901 cttggggaag aagatcacct tggagatatg gactttaaag tttctgggac agaagacggt
    18961 ataaccggct ttcaaatgga tataaaaata gctggtgtta ctacagaaat tatgaccaag
    19021 gcattgagtc aagcaaagga tgcccgtctt catattttaa atatcatgaa taaaacgatt
    19081 tctaaacctg cttcttcgat tagtaaatat gctccaaaaa ttgaatcaat gaaaattgat
    19141 gttgataaaa ttggtgcgct catcggtcct ggtggaaaaa cagttaaagg tatttctgcc
    19201 ctctacggtg ttactatcaa cacagatgat gacggaacag ttacgattta tggtaaaaca
    19261 gctgcagcaa ccgaagatgc aaaacgcgcg gtaaaaggca ttgtagaaga tcctgaagta
    19321 ggctttattt acaatggtac cgtaaaacgc attatggatt ttggtgcctt tgtagaaatc
    19381 ctaccaggaa aagaagggct ctgtcatata tccaaatttt cgcgaagcaa aataaatgcg
    19441 gtttctgatg tcgtaaaaga aggacaacaa atcccggtaa aattgcttga aatcgataaa
    19501 atggggcgct taaatctttc ctatatcgat gcaattgagg aacaagaaaa aaaataaaaa
    19561 tgcctatcgc tcttttgcat cttgataatg gcatagacct catcctcgaa ccgattgtaa
    19621 cggcaaaaac cgccgcaatc ggtttttggt tctcagttgg ttctcgctat gaggcaaaaa
    19681 atcaacgagg cataacacat tttgttgagc acatgctttt taaggggacg agcagtcgaa
    19741 cagcacgaga tatagccctt gcctttgata gaatcggtgg ctatatcaat gcctttactg
    19801 acagggaaaa tctctgtgtg cattgtgttg ttccgagtga atatgcagaa aaagccatag
    19861 aaataatgaa tgatatgatg aatcattctc tgttttcccc tgaggagatt gaaaaagagc
    19921 gacaagtaat tgaaagtgaa attatatctt cctatgatga tccagaagag gctgcaaatg
    19981 aagctgtttt tcaagcaatt tggccaaatc aatctctttc gcaaagcatt gcaggtagtc
    20041 tagaagatat agcttccttg gttcgagaag atttactcga ctggtataca agatatattc
    20101 aaaatggaaa actccttatc tgtatatcag gaaatttttc tgaaaaaaaa attcgagcag
    20161 ccttagaaac aaatccagat agaaaaaagg tttttaatga accgcctttt ttactaccaa
    20221 agccacatga aggttttcat tttttatctg cctcctttca aatggaacaa ttgttttttt
    20281 gctataacct agaaaaacca aaaaccctgc aagaatattt gcattggcat attttaaatg
    20341 ctataatcgg agatacgatg agttcgcggc tctttcaaag cttgcgagaa cgagctgcgt
    20401 attgctacac cgtctatagt tactatgcaa cgagctctga ttctgctctt ttatgtgcct
    20461 atgcttctgc ttcaaaaaag aatgtcaaga aacttatagc agatatgaaa aaagaaatgc
    20521 aaggtctttt tgctctaaat atttcggagg aagaagttga agcttcaaaa caacatcttt
    20581 gtggagaaga aattattgcc tccgaagatc cagagcaaag aatgaagcaa cttgcgcttc
    20641 agtatttttt agatttaccc ttggtaaatt ctgaagagcg gattgaagaa ataaaaaaaa
    20701 caaaggtgga tcagatagaa gcgcttataa aaaataattt gcatgtttca aaatctgcct
    20761 ttgccttgta tggtccgtca ttgggttttt atacgaaacg aatgcttaaa aaaaactata
    20821 aggatgacta tgctaaaaaa taaactaaga atcttgtata agaagaatcc tcacttaaat
    20881 attcctgagt atcaaaccca aggggcagct ggtgctgata tttcagcttt cttagaagat
    20941 gcgattacta ttttacccgg agattttcaa gcaataccga cgggattatt tttagaaatt
    21001 cctgtaggct ttgaagtgca aatacgacca cgctcgggtc tcgcctttaa gttcggcgtc
    21061 actgttttaa atagtcctgg cacaatcgat agcgattatc gcggtgaagt aaaggtaata
    21121 cttatcaatc atgggaagga agcatttgta gttaataatg gagataggat agcgcaaatg
    21181 cttgtagctc ctgttgtgca agctgagttt gaagaaatga aaagcttaag cgaaacacaa
    21241 agaagtggtg gtggctttgg ctcaactgga atcgcataat aaaaataaag cctcgtttta
    21301 ttttagtaag gaaattacaa aatatctttt aaaagaattg cttttgtttt tcctcctctc
    21361 ttttttattt ttcttctttg tcttctttgt gaatcaaata ctattgatgg cacaagatat
    21421 tttaaaaaaa agagcaccac ttgcagatgt tataaaactg attgtctatt ctcttccttt
    21481 tgttatagca caagcggcac cctttgcaac gcttgtcggt tttttaatgt gtcttggaaa
    21541 actagtcagt gataacgaaa tactggtaat acgagcaagc ggtttttctt ttaccgccct
    21601 tgccatacct gttttttctt tggggcttat tatctctatt ttttcttttg ctgttaatga
    21661 ttatctttta cccatgggtt caatagctta taattcactg tataacgaaa taatttatgc
    21721 gaaccctgct gtcgttattg aaccaaatac aattaaaagg gctaatgatg ctgtgttaat
    21781 tgttggcgat ctagaagatt ctgttatatc tgatttattg attatcgata aagactccga
    21841 tggctatacg cgtattattt cttctggaaa aactgatctc ttaaaacctt cgagtaaatc
    21901 tattttaatt aaattgcaga tgagcaatcc tgaaattttt agctttgata ctaataatct
    21961 taaaacctat aattatgcct atggctcaaa ggctatattg catttttttt cagatagatt
    22021 tttttctcag tcgtctacaa taaatcctcg agaaatgact tcctatgatt taagaaaaga
    22081 actaaaaaaa atacaagaaa aaaataaggg aaaaagaaat tttgaactaa cagtctatga
    22141 aatagagtat tataaaaagt tttccctgcc ttttggtgcc ttcttttttt cattgctcgc
    22201 tcttcctctc gcacttttat ttggaaaaca taatggacaa acaattggac tcatagtagg
    22261 agttttgctt tctgttttat attgggcact tctcataacg gggcaaacct atggagcaca
    22321 aacaaataca aatggctttg ttgttatgtg gctaccaaat gctatcatgg gaataatggc
    22381 ttttatttta ttttttaggt tggtaagaaa atgaccttgt ataattattt ggcaaaaaaa
    22441 ttttttccca tattcttatc ttcacttgcg tttttttctt ttgtccttat tctccttgat
    22501 ttacttataa atatttggtc ctatataact caggctgttc ccgtctcatc cattttatat
    22561 gtttcctatc tttatattcc caaaacaatt tcctttgcaa ttcctctggc aattcttttt
    22621 gcggcatcat ttagcttgag tgatttaagt gcaaaaaatg aactatttgc tatttttgct
    22681 tctggaattt ccctcatacg atttactctt cctttactta ttttttcctt tcttgcaagc
    22741 tttggctttt tattcttcga agaccgcatt gttgttccga cttataataa aaaaattgtt
    22801 acacaagatt ttttgttaca aaacgtcgaa agtaaaaata atagtcaggt agttattctt
    22861 ttagataagg gaaaaattgt atacaaggct gattattacg atgatgatgc acagaaactt
    22921 tttggacttt tggttgtttt acgtaatgat gctatggaag tggaaaaaat actgcgcgca
    22981 agttctgcaa gctggagaga agaccaatgg ctgtattctg gggaaactat ttttgaaata
    23041 atcaatcacg aggttctagt aacaaaggat ataagtggtt tggaaatacg ggaaaaacct
    23101 gaagtgtttc gaagcaatgt aataaatatc gaagcagaaa atattcagaa ttcaaaactg
    23161 tatttagagc atcttgaaaa aacaggtttg cctatggcgg aatcgctttc gctctactac
    23221 aaaaaatttt catttccgtt tattgttttt attgttgtgt ttttatcgat tggtgtatct
    23281 ggaaaaacaa aaaaaaatgt tcttcttgta agtcttgctt ctagccttgg ttctgcggtt
    23341 ttgttttatg ttacacaaat gcttactatg cttatggcaa agttttctgt tatatcacct
    23401 cttatgggag cctggttccc tgtattttta tttgtgggta ttagtcttgt attattacgt
    23461 tattcaaaaa catagaagtt tgaaactatg tttttttaag actttaaact aaggggaaaa
    23521 atgaaagaaa tatttacaat agaacatcaa gatgaaaact cacgagcaag aacgggtaga
    23581 cttgtactcc cacacggcag tgttcaaaca cctgttttta tgccggttgg cacaaatgca
    23641 actgtaaaag caattaccgt tgacgactta catgagatag gctttgaaat tattttatca
    23701 aatacctatc atttatatct tcgccctggt tcagatgtta ttggaaatct tggcgggcta
    23761 catggctttt ctcgctggga taaaaatatt ttaactgact ccggaggctt tcaagtattt
    23821 tcacttgctc cctttcgaaa aattagagaa gaaggggtgg agtttaaaag ccatatagat
    23881 ggctcttctc attttttgac tcccgaaaaa atcgtgcaag tacagagaca attaaactcc
    23941 gacatacaaa tgcagctcga tgtttgctct tcttggggag tcgattatgc tgaagcaaag
    24001 gaatcattac gaattacaaa tgattggctt ttgagggcaa aaaaagaatg gaaaaaaacg
    24061 caagcggatg ggtatcaagg gcttttgttt cccatagtgc aggggaattt tttcaaagac
    24121 ttacgagaag aatcagcaga ttttgtaagc aatctagata tgccgggaat tgcgatcggt
    24181 ggtttgtctg tgggggaacc gcttgagata tatcaagaaa tgctttcttt tacggcacct
    24241 cttttgccat atgaaaaagt aaaatacgtt atgggtattg gaacgcccga ctatatttta
    24301 gatgcaatag aaaacggaat cgatatgttt gattgcgtgc ttcctaccag aaacgcacga
    24361 aatggttcct attttacccg agatggcgcc ctcgctataa aaaaagaaga acaccggctg
    24421 agtgcggaag ctattgatgc tgaatgcaat tgcaaagtat gcaaaaatta ttcacgggct
    24481 tatttgcggc atttatttaa aacaaacgaa atgctcagct ctatgcttgc aagttatcat
    24541 aatttatatt ttttacatga catggtaaaa aatgcacgcg aggcaattag aaaaaataat
    24601 tttactgcgt ataagaaatg ttttttagac cgatatttta aacgaggtgc aaaatgaaaa
    24661 aatgccatgg tttttgtatt gttttctttt tcctcttttc gttttttatt ttttctgcag
    24721 gtgcagattt caccgagata gaaaaaaggg aaagaacact tgtctacggt ttggaatctg
    24781 atgttacaag cttaattgat acgcttataa aagaaaaaga ctttacttat acaaatcaat
    24841 tatttgatat atatgtacaa tcacaaaata ttgtcgtgcg agaaaaaata attctctatt
    24901 ttatgaatgc cgaagattat gccttaaaag agcttgtctt agaaacgctt gaagacccat
    24961 gggatgaaaa aaagcaagtt gtaaaagcta tgttacaata tgtgggaaaa ttgaaaatta
    25021 cagaggctgg tccctttatt ttagacctcg ttaaaactga tgatgaagca tattttgaaa
    25081 atgcggtaat tgctttggga ttagtcggcg gcgaagaaga agccttgtat cttgcggagt
    25141 attttcaaaa tgaagcaccg agtattataa aaaaacaagc tttgatgaag gctctcgtaa
    25201 acatgaatga agaagcgatt tgggattttc tcgttgaaac aatgcaaaat gaagatgaaa
    25261 atacctacgt gcgaatgtat gcgagcgagg ccttggggaa tacaaaaaag cctgaagctg
    25321 ttgacctgtt aatcgatatg tttagtaata gtgatataaa tattcgagtc tatgctatta
    25381 aggggcttgc aaattttaat gatgaaaaaa cgatacaggt acttatagaa gccttaaaag
    25441 ataataatta tagggttcgg ctcgaagcta tagaagcatt taaaaaaaat aaggacgagc
    25501 gagcagcgga ctctctttta tatcgagctg agcatgaccc cgaaatgagc gtaaagcaaa
    25561 agtcttatga aacacttgct attctcaata cagaggctgt tcaacgcttt ttcaaaaaag
    25621 caattgaaaa tgctaagcac aacactgacc ttcgcataaa ggcggcagcg gtttctcttg
    25681 agcaagaaat agaagcagtc tatcagtcta tttttgatct tgcaaaagaa agtttaaaag
    25741 atgataagtt aaaaaacata cgctatgcct tgggtaaaga gtttgcaaag tataaaaata
    25801 aagcctttga agaagtttgt gaattatatt tatctcatac agatgtttca acaaagggaa
    25861 caggattgga tatttttatt acaaacgctt atgtgagtct tgttccaaaa gtgcaggcta
    25921 ttgcaaacaa cgacaaagaa ggtgcaaacc aaaaaaaagc ccgtcttgct ttagaaaaga
    25981 caggctttga tttgaaataa tttatttttt aatatatttc tttttatcgt cttctggaat
    26041 aaccgacaag agtagttcat tgagtttttc ggtttgagac tcatattcgc taaacataat
    26101 acgtgtgatg taaccatgat cagaaataat tgtaatcgga gtgtttaagc aatcttcaat
    26161 gatatttgaa tcagcttccg ggaaatattt tggactaaga agtgctaaac tacagctttg
    26221 accatcatag cgtatttcag caatattcgc gggaacttta ataaagaaaa ttgagaaaac
    26281 tgatagacca ccaccgatcg attttctagc tccagctgat aaggcatgta tatttcgtga
//
LOCUS       DAOSSW010000014        25831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_2094_length_25831_cov_6.440022, whole genome shotgun sequence.
ACCESSION   DAOSSW010000014 DAOSSW010000000
VERSION     DAOSSW010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 25831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25831
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2094_length_25831_cov_6.440022"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PKW26_02390"
     CDS             <1..724
                     /locus_tag="PKW26_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92448.1"
                     /translation="KDEAHLNQALGIFFPFLFGDKIQFTIKPGFMYLYSLALEGSHTS
                     SSYLIPTFDIETKKISYTGNFRNGYEVSSETNFDFHLEKKRFNPNLLLQAEFFTPVLK
                     KDEPFFLHSARLIYYFNKERDIPMGFFIRGILDTELMAKHAIVINNDFTFKLFNTRFN
                     TKHSSKFNFELQTSLFFDTSFVFTPEYIFDQTMGLNLIVQPESMKSVQGRISVAGNFD
                     WTNPKKDRKSGIEIYIGIGLFY"
     gene            complement(731..>1749)
                     /locus_tag="PKW26_02395"
     CDS             complement(731..>1749)
                     /locus_tag="PKW26_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92449.1"
                     /translation="ISWQLLLDAEQAFYNREYGTALKLANTALVNRQKEVDWSLRVLD
                     TATKSAAFHSKDALDEIYEMLLERDEHDAVLLLESIFIKYGLEDFDNSLSQLRVFIQD
                     QKEYPEVYFLIAKIYMLEGEWKLAEDFLLKSYKYASHLIVQSQKYDILYALFDLYTIT
                     KEIEKQEKTLLLIIADDPHYSQNYKSTSLFYTAAQAIRTNTELNKFFLLYHSQSLFSM
                     RAYYELAKMYSVDAENKGLVLQLLSLAAISAYDRIETIIKNRVFDYSFDSFDTLLRIA
                     LKYDDILDWMQSENVWEIFYNLALSANENKNASFAKTLLIHISNYCPDNYWKKRAIEA
                     YIQF"
     assembly_gap    1750..1849
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1850..2832)
                     /gene="ispG"
                     /locus_tag="PKW26_02400"
     CDS             complement(<1850..2832)
                     /gene="ispG"
                     /locus_tag="PKW26_02400"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOQ92450.1"
                     /translation="MISRTVQIGAGHVKQIGIGGFSPVSIQTMWKEGILVVLKESKEY
                     NKDALFSILERIAVLESLGCDLLRFAVPDMESAQALNIIASKTSMPLVADIHFDYRLA
                     LECLKGDVAKIRINPGNIGKKENLVQVVEACKDKNVGIRIGVNFGSLPKDLLARLEEP
                     GDKKSDTNEDAKVFKVEDALVEAAERECAVLENLGFYNYLVSMKASNVRETIAANELF
                     ASRNNIPLHLGVTEAGPMIPGIVKSTIAFTHLLSKGIGNTLRVSLSDSPENEVICARE
                     ILSENGLRMGGVKIVSCPRCGRNGFDVHAFVNKWQNELLGLDKNLQIAVMG"
     gene            complement(2835..3260)
                     /locus_tag="PKW26_02405"
     CDS             complement(2835..3260)
                     /locus_tag="PKW26_02405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701618.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOQ92451.1"
                     /translation="MEFLSWLGTACVFGISAIGSAIGIGAAGLAAIGAWKRGYLNNKP
                     APFILSVFAGAPLTQTLYGYLLMGALQEKLLAGANPGLILGTGVFTGLVIAASAIFQG
                     KAGAAGADALGETGKGFANYLMVVGLCETVALFAMAFNF"
     assembly_gap    3385..3484
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3764..5725)
                     /locus_tag="PKW26_02410"
     CDS             complement(3764..5725)
                     /locus_tag="PKW26_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOQ92452.1"
                     /translation="MKKVSLVVLNRYKEKSLKSLRKLGLVHLETLEGSSEELSRVRAL
                     YDRLSTAKTSIHDLKLPKKYTSPGKKSAVVENLDRDAILSAYEEKGKISEGLKKALLQ
                     AEEILALQEAKKEIGEDIQLARKELDRLAAWGGVNPQDFAYLEEKGIGLTMYEIPSAA
                     LKKIPDETTILQVNADKNATRFLLLQFAGEEKAELPPEAFKIALGEESTQSLEKELAE
                     KNKALLLIQNDLYEALQYLPNLSEEMQNLSKAIEFENIYAAMPQDEGLDAVETAVLQS
                     ENRALTWITGFIPTEDIEALRNFAQKEKIALIITEPKEEDEVPTKLKNNKVVSLIYPV
                     TDFLGTVPGYREYDISGWFLLFFSIFFGMIFGDGGYGILLVLTGLGLILHSKIKGKKI
                     EPMIILVALLGLSTVVWGTLTCTWFGLEVSQIPAWLVNLSWEPISNANPNEELLKQNI
                     KIFCFALALVQLSIAHLKGVFANIKSLKFLGELGSLILLWGVFYVVLNIVVDKERFAF
                     DAVLFGGYEAMPIVLSAIGIGFAMSFIFANYEGNIFASIMASVKDIISVLLGVVNVFS
                     DIVSYIRLWAVALAGSAIAATINTMAGPALGSALMFLGILLLIFGHGLNMVLNVLSVL
                     VHGVRLNTLEFTGHLGMSWSGHSYEPFSE"
     gene            complement(5737..6354)
                     /locus_tag="PKW26_02415"
     CDS             complement(5737..6354)
                     /locus_tag="PKW26_02415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOQ92453.1"
                     /translation="MAIKLTKNELKTQKDALKRYTRYLPTLTLKKQQLQSEIRDIEIR
                     AKELRKAREKLEKEFSSWIAVFGETGFFKPEMVVVSNIKKGFTNIAGVTIPVYEGADF
                     ARGDYDLYLTPLWIDLAADRMEKALAYDLEAEVLEEQIRLLSRELRVTTQRVNLFEKV
                     KIPETKAHIKKITVYLGDQQVAAVVRGKISKKNLEAKTNTFMEIA"
     gene            complement(6367..7659)
                     /locus_tag="PKW26_02420"
     CDS             complement(6367..7659)
                     /locus_tag="PKW26_02420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOQ92454.1"
                     /translation="MKKIYSKIESINGSVITVRAKDIKYGELAEIQTSFGASLAEVNK
                     IDGDLVSLQVFAGGRGVSTGDEIRFLGHEMQVSFSDDLLGRIFNGAGEPRDKGPLLSE
                     NLVEIGGPSVNPSKRIIANRMIRTGIPMIDMFNTLVVSQKLPIFSISGEPYNELLARI
                     AMQAEVDIIILGGMGLKYDDYLYFKNTLEEAGSLSKTLMFVHTASDPTVECLMVPDMS
                     LAVAEQFALQGKDVLVLLTDMTNFADSMKEIAITQEQVPSNRGYPGDLYSQLAARYEK
                     AVDFDDAGSVTILAVTTMPGDDVTHPVPDNTGYITEGQYYLKGGRIEPFGSLSRLKQN
                     VNGKTREDHRALMDGMIRLYASYKDTLEKKAMGFMMSQWDEKLLKYGELFEKEMMDLS
                     INIALEDALDLGWKILADCFEKEETGLKSDLIAKFWPQ"
     gene            complement(7662..9431)
                     /locus_tag="PKW26_02425"
     CDS             complement(7662..9431)
                     /locus_tag="PKW26_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOQ92455.1"
                     /translation="MTDTKGRVVAVNGNLISVAFDGTVSLNEVGYVSVGDKKLKSEVI
                     RIQNGEAQLQVFEMTKGIQTGDEVFFTNELLSIELGPGLLGQVFDGLQNPLPELAEQA
                     GYFLERGVYLDPLPDASTWSFTPIAKAGDTVKAGSFIGEVPEGTFKHKIMVPFTFLGN
                     YTVVSVKEKGDYALKDTIAVVKDARGVEHNLSMSFNWPVKRSVDCYAERLMPTDTLTT
                     KIRLIDTFFPVAKGGTYCIPGPFGAGKTVLQHTTSRNADVDIVIVAACGERAGEVVEI
                     LKEFPELTDPRTGRSLMERTIIICNTSSMPVAAREASVYTGVTLAEYYRQMGLDVLLL
                     ADSTSRWAQALREMSGRLEEIPGEEAFPAYLESYIAAFYERAGIVRLHDGSTGSVTIG
                     GTVSPAGGNFEEPVTQATLKVVGSFHGLSRERSDARKYPAIDPLESWSKYKSVLDKDW
                     VDFARYYMHRGNEIEQMMKVVGEEGTSLDDYIVYLKGEFLDAVYLQQNSFDEIDSAVT
                     VERQNYSFQKIIQVLGSDYNLDSKDDARTYFYQLRQSFIDWNYVLWGSEEFSKKEKDL
                     DALYESKVKKLSDKAKHLLEGAK"
     gene            complement(9428..9970)
                     /locus_tag="PKW26_02430"
     CDS             complement(9428..9970)
                     /locus_tag="PKW26_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92456.1"
                     /translation="MSFYYLMSQLPYFSLSQSSPLPITESYFLELAERNLNEAQLKTL
                     KNISLVPEKKPREIDSSFLTAYYAWERELRFVLGQIRGQRLKKDFSLPNNLSQSFEIL
                     SIARTATGFESPLEAEQFLNEQRFLAIKKLQPMDYFSLDSVYAYALQLKLALRIKQFN
                     EEAGLAEYRSMYDTILGESK"
     gene            complement(9972..10595)
                     /locus_tag="PKW26_02435"
     CDS             complement(9972..10595)
                     /locus_tag="PKW26_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HOQ92457.1"
                     /translation="MDIQLQELIDKIKLDGVATAEEEAAKIISEAEKSAQGIVQEAEK
                     NAARNIEKAKLEAERLEKASVDAIAQASRNVLLTFRENVVKELNALLKDAAQSALSAD
                     LLKSLIPEVIKNWTEKEDAKDVSVLLNKKDLDSVQSSLTASLKAELAKGLRIQMSEEV
                     VDGFRLSLKDGEAFYDFSSESIASLFSSYLNPQTAKIMKEASQTMGN"
     gene            10772..11236
                     /gene="smpB"
                     /locus_tag="PKW26_02440"
     CDS             10772..11236
                     /gene="smpB"
                     /locus_tag="PKW26_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOQ92458.1"
                     /translation="MKKGTKLIAQNKKARFNYHIEEDYECGIELKGTEVKSVKNGSLS
                     FPDGFAEIIDNEVWLKNFSIAEYPFSKHFTHEPQRPKKLLLHRAEIKRLKKKTDEKGY
                     TLIPLDVYLKNGLVKVRLGLCKGKKLFDKRDTIRQNDLHREVQREFRKNLEG"
     gene            11245..12081
                     /locus_tag="PKW26_02445"
     CDS             11245..12081
                     /locus_tag="PKW26_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92459.1"
                     /translation="MKKSLVITITIFLLLPCVFSQTRTKELGFFAPIYNTKDEGTAKL
                     IVDLYYNHLLSFDSFRVQDFRSIVFSKDNILENYGIQLAFFIDISDSDNNWTIAFNTF
                     FDNKTHTEEKIYDSYYQVLLSAKNDLFVFLDRLEKTEENTAREKSDDVSATTVSINLD
                     SIAGTWFTDASIDKIIILRGGRGFVIYKNGASMNISVEIRGNQIIALQTSKTNASFFP
                     ELPRDLALKVATEELQPIKWIMTAKNENEMVGEKNHYEAVYENDKATSVKTVSSPSRW
                     TR"
     gene            12081..13574
                     /locus_tag="PKW26_02450"
     CDS             12081..13574
                     /locus_tag="PKW26_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOQ92460.1"
                     /translation="MIKFNPQNPLDALEAWLDTYLNFEKMPKKDFFWLDTMQFLCKKC
                     NNPQNTFQSFHVAGSKGKGSVSTFIASILTEAAIDCGLYTSPHLCAFSERVTKSHAVL
                     DDSIYANAYKELSYTIDSIIPKTYPNQREPSWFELATLFAFISFKEAGFPWAVLETGL
                     GGRLDATNVILPAASVITEIELEHQEYLGNTVEAIAREKAGIIKKGIPVFCSSTEAIA
                     CAVFKTEAKKKDADYFHLDDFIDKVQYSYHEDKMKIEASMPELFSRPLKASLQLLGAY
                     QVRNALLASLVVKKILPQISQRQIETGLENAFIPGRFQIIKKTKQNPLIVADGAHTKK
                     SISHCLSTLQELFLSAPKKDSSKQRVEPRFSLLFACAMDKDVEAIAHLLVESKLFTRI
                     FLTKPGAVKQTNLEKANEAFEKAVNAEMLQGSRYGSSKNSSENQIEKSEVKPAFFLIE
                     SYEEAMEKAFQEALKQDETLVVTGSFYLVSELHSFLANSIRSSSNSA"
     gene            complement(13508..14422)
                     /locus_tag="PKW26_02455"
     CDS             complement(13508..14422)
                     /locus_tag="PKW26_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOQ92461.1"
                     /translation="MLVNAQGKIAQKEFSKSIDLKKSQIFFDAEDYLIYYKAAGLASA
                     PLKKINSEDLSKDFLEEGEEDSALREVAKIYPEVLEVASQFKPIEGGLVHRIDTATSG
                     LLLLAKNQKSYDFFIDIQKKTSFRKCYTAFSHKISQKDVKKSLKRGYPPLPGEYRLEE
                     TMQLPLKIASAFRNFGPRGASVRPLLIENAKDKKIYQTSIDALSHLGFARKIGVSLSL
                     AYRHQVRSHLHWLGYPIIADMRYGRESDSDRIGETHKNPKKSQKPMLFFASGLSFIDP
                     KTGKTVSYALFEEDLIEFARKECNSETR"
     gene            complement(14450..15436)
                     /locus_tag="PKW26_02460"
     CDS             complement(14450..15436)
                     /locus_tag="PKW26_02460"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOQ92462.1"
                     /translation="MIDIESKVSLAEGERLRLDLYLITQLDPSFSRSRLKEGLVLLTC
                     NNKNAKLSSRVKNGDTIHLQWKNPIPSTIKPEEIDLNIVYEDENLMVLNKAQGMVVHP
                     AHGNWSGTLVHALLFHWGREVIQKEANLLDWPEAQKRPGIVHRLDKDTSGLIITAKNT
                     KTEAYLKKQFKKHSVIKQYIAITKGHPPFEKGTIKTQIMRDPNNPQKFIASTDESQGK
                     FAHTDYICIALYGPYALMKIRIKTGRTHQIRVHMKYINCPILGDPLYGVRDSRFKTAT
                     LMLHASKLGIRLPEQKQRSFFKAATPIRFKKVLQVFHKEYERNSMWMKKHKS"
     gene            complement(15437..15868)
                     /locus_tag="PKW26_02465"
     CDS             complement(15437..15868)
                     /locus_tag="PKW26_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92463.1"
                     /translation="MKKTLVFFLIISFSFSLLWADNSPEPYAKDEFKPWMKQLRRSEI
                     VTLGSLPFTTLTTSLGYSFYRYAANDFDSAYFPNPFSPSQANLSTEEQKQVLFISLSA
                     SLLVGIIDFSISMIKQNKVKEEKVKTSEPYTIKRIVIEADE"
     gene            complement(15901..16671)
                     /locus_tag="PKW26_02470"
     CDS             complement(15901..16671)
                     /locus_tag="PKW26_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOQ92464.1"
                     /translation="MNDKKESDITIYEKNIKNALNEILENIEKAALKSARKSSEVSIL
                     AVSKFRSIEEIQAAINVGLELFGENRVQEAIEKFPSILEKNPQVKLQMIGSLQRNKVK
                     HLLPIATCIQSVDRIELIKEIIKEVKKRNEVSASDSQPIELLFEIHTAEDSKNGFKDL
                     DELWLCLDYLLSENEPRLLAQPAGFMTMAAFTKDEKLIRSSFQKLRSVQEEAKKRYPN
                     LHLDVLSMGMTNDYAIAVEEGATMLRIGSALFIPSFFA"
     gene            complement(16673..17563)
                     /gene="rsmA"
                     /locus_tag="PKW26_02475"
     CDS             complement(16673..17563)
                     /gene="rsmA"
                     /locus_tag="PKW26_02475"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOQ92465.1"
                     /translation="MTFLPDFNSPTAIKLFLESHEMAMQKKFGQNFLIQESSRKKILA
                     AFGNVEGKKIWEVGPGLGAMTQHLLEEKATLIAFEIDKGFSSVLRKAFSPERILAPAS
                     FRLVEGDFLKTWENEWETQGAPDYFFGNLPYNISSQILANTISKGLRFEKAIITVQKE
                     LAERIDAKPGQKDYSSFSVLIQWAYNVKKIIDLGPSHFWPRPNVDSRSLLLEKKEDFP
                     QCLDAGHFMELQRALFLSRRKTIKNNLIQFYKNDDIAETVLENANIKANERAENLSLE
                     NILYLSDCSYQFLQEKKGGK"
     gene            complement(17611..18936)
                     /locus_tag="PKW26_02480"
     CDS             complement(17611..18936)
                     /locus_tag="PKW26_02480"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOQ92466.1"
                     /translation="MKAILTQLSVHLRRLWQSLIGSAKNTSLIEKLALVVILMYYLFY
                     ASIPVRKPFYTVFPIDTIDRLSLCLLESPQKVNEQYFLLRAQVLEVGMQRQNLTFSHE
                     GEGRLEALVPAALVESLYPGRLFSSTGSKAVFESGSILDCSGSFTKKSMNTKKTAVPT
                     FFIKKAEQRGWKSGLSQFRALLRLAFKRLLYTWGEGGSLVLALLSGERLYLEPRLADA
                     FRNAGLSHILALSGMHLSFFAGLTAGFASRIFGKKRAPFFSFLAISFFVWFAGFSPSL
                     MRAYICALIGLLSSFASLDINLTSVLSISFILQILIAPDDGMSLSFLLSYSALLGIAL
                     FSRFFFSKCIRFFPEPIALSLSASLAAQIFTIPLTLAFFGTITPIGIIATVFISPLIS
                     LFMSLSITAIILALCLPQTLFFSGVLIQGLYSFIAFLVVLFAKVPAIQF"
     gene            complement(18872..20047)
                     /locus_tag="PKW26_02485"
     CDS             complement(18872..20047)
                     /locus_tag="PKW26_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92467.1"
                     /translation="MKKLYFTILFFLSTVFLFADFSQEQKAFVSSSIQDKVSILKKQI
                     AILEKDLPLLDMALDFCLTNAAILSDSTHLHDLLFVSLYALPQDNLEPYDSALKKLFF
                     LYNKEEIRVEILNLLTKIAISEAFFTENLYNFLFEEIEKDYSRRSNKSIFASLQYLSS
                     IQDLAFFNKIYPLLSKNIDFELKKNIEEVLALSIENYKNDLLERISTTTAQEKKLILS
                     ILGRNPHLNLFFKAEVTENLLRATIISIGDNTGTERESLLKEFYEVQMESVRIIKEAK
                     WTRAASLVAGYFPIAVEQYTAFLISKDELLEVMDSLTLLATSETGRALSDYLAVLNKK
                     TEKTGLFDEEIMLHLISALAQLGEKTAFDNLLYVILHEGYPDSIISASKEALAKLNW"
     gene            complement(20044..21141)
                     /locus_tag="PKW26_02490"
     CDS             complement(20044..21141)
                     /locus_tag="PKW26_02490"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOQ92468.1"
                     /translation="MAILRHFKDMIRFFSMGFDSSFKFRQIHMLWTLAKNANLEDPTS
                     LYFSIPVLDRTISQLLTIARAKGQDKTDSFNAFLTKLYEFRTEVELSLGKKGLESTKE
                     LDVGQVLRIILTGKGVFTSKILANGRNLIIEVPKKDKTISITGLDWIGKDISVYLWRK
                     NDAAYVFDTAVLDSSLYNGQNVLYLAHTTALMRTQNRKSIRVPCKIPAQIFIVNLAVS
                     DPFAMEHSQGLKCMLEDISETGALIRIGGEGLKDMEIKLQFQLNDSLVIMAGLVRNVK
                     YDAKLNQSLLHFEATLINPSMKNTILSYVYNVLPQEEKDVLDVMNLVEEDENQVSEEA
                     EGLDKEIDFGDTELGETMDMPTESFFQGEME"
     gene            21229..21864
                     /locus_tag="PKW26_02495"
     CDS             21229..21864
                     /locus_tag="PKW26_02495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOQ92469.1"
                     /translation="MKIQHFFYQISEFFIILILFVLPSIFSKEMTTFTNTKEFFSFVI
                     YLFFSIFLLFILNKKNKTEKKTQKKPILVSIVSLILLLWLSFIFSVSLPVQEKSYIKP
                     SLFVQNLFFIPILVSFEEVLFRAYIPDKVFYLFTIKAKKQSRFIKLLAIVFSSLLFAL
                     AHSYAGFYSIIFAFLSSLILFVLKDYTSSLFFPILVHSLYNYFAFFYLLKT"
     gene            21920..22981
                     /locus_tag="PKW26_02500"
     CDS             21920..22981
                     /locus_tag="PKW26_02500"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOQ92470.1"
                     /translation="MTLYILALIIAFGISTISMPIIIKISKKRNLFDRIDDRKVHTGQ
                     ISRLGGIGITIGFSIALAILYFSGLFSTIQQNIWYLLGAGLCISAMGIIDDLKALSAK
                     LKLLIQVVAAILVLIGGFKFTGIAISSVFYLNFSFFSYIITFCWIIGVTNAYNLIEGI
                     DGLCGGIASFASLTFAFIFFKSDNTAAIFICLSLASAILGFLLFNWPLPKAKIFMGDG
                     GSQFLGFVLAVLPLLSSKGSMPTISLPYAIAVLMIPIFDTIAAIWRRLREKKRIDSPD
                     RFHIHHKLMLLGFSSRSTMVILYLLQAIICIFLASAYWLGGYISVFILLADYLTGVLF
                     FTIIHIGKNQALKTDTQEA"
     gene            complement(22956..23522)
                     /locus_tag="PKW26_02505"
     CDS             complement(22956..23522)
                     /locus_tag="PKW26_02505"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOQ92471.1"
                     /translation="MRILKNDSKNYISILRALENDKVIVLPTDTIYGFSGKINTTKEK
                     IQAIKGREENKPFIVLIEKPEDISLFSDIRPHQDLLALWPGALTIILPLRNSEESIAL
                     RCPGDAWLRKLIADLKSPLYSTSCNRSGLPPLSDIDEIEKEFSKEVALIVEDPSSGSG
                     LASTIVSIEEHSVKLIRQGSLMLPAYLF"
     gene            complement(23509..23979)
                     /locus_tag="PKW26_02510"
     CDS             complement(23509..23979)
                     /locus_tag="PKW26_02510"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOQ92472.1"
                     /translation="MRIKRILISIALGTLCYVGISFVFGESGVLAEKQLIEQKDMLVA
                     NINTIQKTQDSLILKQKALTEDTEVIASYAKQIGYISEGETLLKISGIPSNKAKQHEI
                     GKKYLKTEIFFVEERISKTCGIVIFILSYLVLFLFSLKNKSFSNKQERFLHAYS"
     gene            24127..>25831
                     /locus_tag="PKW26_02515"
     CDS             24127..>25831
                     /locus_tag="PKW26_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92473.1"
                     /translation="MSLEENNKIDQYGVWLKKTPLLDEKEEQTEIEVEDMSENMSDDF
                     IPIDDSFDTGKNAEVAESASLDVTEENIQDDYSDFIEQSKEDIFDEEMSSLLETETSV
                     DQEILDTAPEDVSLETFSDGEIDLSAFMDESEKEKDNAEPSKTEEVSLDDFSDGEIDL
                     SAFMDNDSIDSSSKSTSEEINIDDFFSGTESLHVDSFLTNEAPKEDIFEEDPIDIDLV
                     FDDAFASEAISNPSEDIEIFYTSDSDAEFESFNVDDLFNDTEELFETEKISPQTVTYE
                     DANEFDDLLSSLDESSPSVIDTEVTKAKKATQVYNISVRVDDDFEDKENIMEKPTEKE
                     NIENKRDETLDDIPLFLNNQKFTTKKDLEKNTSEEYTNLTRLNKEASMEFDELDDILE
                     NVEIIDGTEDTSTKKNLSEETELVIVNETNPLDEEENLDLGEKSTIDADLNMEEDDTI
                     LENAETEVAMEDSTDTSIDTDIELDETIQFNLDEDRQEINETEIFFEDEDDEISIEID
                     MPEDDSSEAISTDIEDALYEEKTEIEVDNKLDNDNLDSINEIDNLASMSEDFDDSLDT
                     ME"
BASE COUNT         7939 a         4980 c         4650 g         8062 t
ORIGIN      
        1 aaaagatgag gctcatttaa accaagcgct cggaattttt ttcccgtttt tattcggcga
       61 caaaatacag tttactataa agcccggctt catgtatctg tatagcttag ctttagaagg
      121 ctctcataca tcttcatcct atcttattcc aacttttgat atagaaacga aaaaaatttc
      181 ttacacagga aattttcgaa atggctacga agtaagcagc gaaacaaact ttgactttca
      241 cttagaaaaa aaacgcttta atcccaacct tttactacag gcagagtttt ttacccctgt
      301 cttaaaaaaa gatgagcctt tttttcttca ttctgctcgt cttatatatt atttcaataa
      361 agaaagagat ataccgatgg gtttctttat tcgcggtatt cttgataccg agctaatggc
      421 aaagcatgct attgttataa ataatgattt tacatttaaa ctctttaaca ctcggttcaa
      481 tactaagcat tcttcaaaat ttaactttga attgcaaaca agtcttttct ttgatacgag
      541 ctttgttttt acaccagaat acattttcga tcaaacaatg gggctaaacc tcattgtgca
      601 acctgaatca atgaaatctg ttcaaggaag aatttctgta gcaggaaact ttgattggac
      661 aaatccaaaa aaggatagaa aatcgggaat agaaatatat attgggatag gtttatttta
      721 ttaaacgact ttaaaattgt atatatgctt ctatggctct ttttttccaa taattatctg
      781 gacaataatt gctaatatgt attaaaagtg tttttgcaaa gctggcattt ttgttttcat
      841 ttgcactgag cgcaagatta taaaaaatct cccatacatt ttcactttgc atccagtcaa
      901 ggatatcatc gtattttaaa gcaattcgca aaagcgtatc aaaactgtca aagctataat
      961 caaagactcg gtttttaatt attgtttcta tgcgatcgta ggcagaaatc gctgctaaac
     1021 ttaaaagttg caagacgagt cctttgtttt ctgcatccac gctatacatt tttgcaagct
     1081 cataataagc ccgcatagaa aaaagtgatt gggagtgata taaaagaaaa aatttgttaa
     1141 gttcggtatt tgtgcgtatt gcctgtgctg ctgtgtaaaa aagtgaggtc gatttataat
     1201 tttgactata atgtgggtca tccgcaataa tgagtaaaag tgttttttct tgtttttcaa
     1261 tttcttttgt tatagtatac agatcaaaaa gtgcatataa aatatcatat ttttgtgatt
     1321 gcacaattaa atgagatgca tatttatagg actttaacaa aaagtcttct gcaagtttcc
     1381 attcaccttc gagcatataa atttttgcaa tcaaaaaata tacttcaggg tattcttttt
     1441 ggtcttgaat aaaaacacgt aattggctta aagaattatc aaaatcttct aacccatatt
     1501 ttataaaaat actttccaat aacaatactg catcatgctc atctctttcg agaagcattt
     1561 catatatttc atcaagagca tcttttgagt gaaaggcagc cgattttgtt gctgtatcta
     1621 ggacacgcaa tgaccagtct acttcttttt gtctgttcac aagcgctgta tttgcaagtt
     1681 tcaacgctgt accatattca cgattataaa aggcttgttc agcgtctaaa agcaattgcc
     1741 aagaaatttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc aacccattac
     1861 cgcaatttgt agatttttat ccaaaccgag caattcattt tgccatttat tgacaaaggc
     1921 gtgaacatca aaaccatttc ttccacaacg tggacaggag acaatcttta cgccacccat
     1981 gcgaagccca ttttcagata aaatttctcg ggcacatatc acttcatttt ctggactatc
     2041 tgaaaggctc actcgaagcg tatttcctat ccccttcgat aaaagatgcg taaaggcgat
     2101 ggtgcttttt actatgcctg gaatcatagg acccgcctct gtgacaccga ggtgaagcgg
     2161 tatattgttt cggctcgcaa aaagttcatt tgcagcaatc gtttctcgca cattgctcgc
     2221 tttcatcgaa acaagatagt tataaaaacc aaggttttct aaaacagcac attctctttc
     2281 agcagcctcg acaagggcat cttctacctt aaaaaccttt gcatcttcat tcgtatcaga
     2341 ttttttatct cctggctctt ctagccttgc aagtaaatcc ttcggtaaac ttccaaaatt
     2401 gaccccgatg cgtatgccta catttttatc cttgcaagct tccacaactt gtacgagatt
     2461 ttcttttttc cctatattgc ctggatttat acgaattttt gcaacatcgc cttttaagca
     2521 ctcaagcgcc aacctataat caaagtgaat atcagcaacg agtggcatgg acgtttttga
     2581 ggcaatgata ttcaatgcct gtgccgattc catatctggc accgcaaaac gtaaaagatc
     2641 gcaaccgaga ctttctaaaa cagcgattcg ctccaaaata gaaaaaaggg catctttgtt
     2701 gtactccttc gattctttga gcacaacgag gatgccttct ttccacatag tttgtattga
     2761 cacaggagaa aatcctccta tgccaatctg ttttacatgc ccagctccga tttggactgt
     2821 gcgacttatc atttttagaa gttaaaagcc atagcaaaga gggctacagt ttcacaaagc
     2881 cctacaacca tgaggtagtt tgcaaaacct tttcctgttt caccaagagc atcagcacca
     2941 gcggcaccag cctttccttg gaaaatcgca gaagcggcaa taactaaacc agtgaaaaca
     3001 ccagttccca agattaaacc tggatttgca cctgctaaca atttttcttg taaggcaccc
     3061 attaaaagat aaccgtacaa agtttgtgtt aaaggagccc cagcaaaaac tgataagata
     3121 aagggagcag gtttattgtt gaggtagccc cgtttccatg caccaattgc agcaagacct
     3181 gctgcaccaa taccgattgc tgatccaatt gcagaaatgc caaatacaca tgcagttcct
     3241 aaccaactta aaaattccat acatactcct ataaaaagaa aataaaaaaa tttcaaattt
     3301 tttattttct tttttctaac tttcgcaacg aagtgagaaa gttaatttat atatttttag
     3361 cgacatggaa gtcgcattaa aaccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnngaagtg agaaagttaa tttatatatt tttagcgaca tggaagtcgc attaaaacca
     3541 cttgccttgt tttgtttaca aaacaagata gtattttttt tcatggacga aaaaaaatac
     3601 gtaagactcg agttgcagtg ctcggcgcta ttttagccga gcactgcata cttactccta
     3661 taaaaatttt ctaactttcg caacgaagtg agaaagttaa tttatatatt tttagcgaca
     3721 tggaagtcgc attaaaaccc acttgtcttc ttttgcaaaa attttattca ctaaatggtt
     3781 cgtaggagtg acctgaccaa gacataccga gatgcccggt aaactccaag gtgtttagac
     3841 ggacaccgtg aaccaaaacc gacaatacat tgagcaccat gttcaatcca tgtccaaaga
     3901 ttaaaagcaa gatgcccaag aacatgagcg cagacccgag agcgggacct gccatagtat
     3961 tgattgttgc agcaattgca gaacctgcaa gagcaacagc ccaaagccga atatacgaaa
     4021 caatgtcaga aaacacattg acgacgccga gcaagacaga aataatatct tttacgcttg
     4081 ccattatgga ggcgaaaata tttccttcat agtttgcaaa gataaaactc atggcaaaac
     4141 caatgccgat tgccgaaaga acgatgggca ttgcctcata tccgccgaat aaaacagcat
     4201 caaaggcaaa gcgttctttg tcgacaacga tattgagaac aacgtagaag actccccaaa
     4261 gcaagatgag tgagccaagt tcgcccaaga attttaagct ttttatattt gcaaaaactc
     4321 cttttagatg tgcaatcgat aattgtacga gggcaagggc aaagcaaaag attttaatat
     4381 tttgcttaag cagctcttcg tttggattag cattcgatat gggttcccaa gaaaggttta
     4441 caagccaagc tggtatttgt gaaacttcaa gtccaaacca tgtacaggta agggttcccc
     4501 aaaccaccgt tgaaagccca agcaatgcaa ccaatattat catgggttct atttttttgc
     4561 cctttatctt cgaatgaaga ataagtccca aaccagtgag tacgagcaag atgccgtagc
     4621 caccgtcgcc gaaaatcata ccaaagaaaa tcgagaagaa aagcaaaaac cagcctgata
     4681 tgtcgtattc tcggtagcca ggaacggttc ccaaaaagtc cgttaccgga taaatgaggc
     4741 tcaccacctt gttattttta agctttgtcg gaacctcatc ttcctctttt ggttctgtta
     4801 ttatgagcgc tattttttct ttttgtgcaa aatttcgtaa ggcttcgata tcttctgttg
     4861 gaataaaacc tgttatccat gtaagtgcac gattttcact ctgtaaaaca gctgtttcaa
     4921 ctgcgtctaa gccttcatct tgcggcatag ctgcatagat attttcaaac tcaatagcct
     4981 tagaaagatt ttgcatttct tcgcttaggt ttggaagata ttgtagagct tcgtacaagt
     5041 cgttttgaat aagaagtaga gccttatttt tttcagcaag ttctttttcc aagctttgag
     5101 ttgactcttc accgagggct attttaaagg cttctggtgg aagttctgct ttctcttctc
     5161 ctgcaaattg aagtagcaaa aatcttgttg catttttatc tgcatttact tgtaagatag
     5221 ttgtctcatc gggaattttt ttcaaagcag cgctagggat ttcatacatt gttaagccaa
     5281 tacccttttc ttcgaggtag gcaaagtctt gtggatttac tccaccccag gcagcgagcc
     5341 tgtctagctc ctttcttgca agctgtatat cttcacctat ttcttttttt gcttcttgca
     5401 aggcgagaat ttcttcagcc tgcaaaagcg ccttttttag cccctctgag attttgccct
     5461 tctcttcata ggcagaaagg attgcatcgc ggtctaaatt ttccacaacc gcgctctttt
     5521 tgccagggct tgtgtatttt ttgggcagct tcaaatcatg tatagaagtt tttgcagtcg
     5581 aaagtctatc atacaaggca cgaacgcgag aaagctcctc gctcgaacct tcaagtgttt
     5641 cgagatgcac aagaccgagt tttcgcagag atttgaggct cttttctttg tatcgattaa
     5701 gtacaaccaa agatactttt ttcatagata ctatcattat gcaatctcca taaaggtatt
     5761 tgtcttggct tctaagttct ttttagaaat tttaccacga actactgcgg caacttgttg
     5821 gtcgccaagg tagaccgtaa ttttttttat atgtgccttt gtttcaggaa tttttacctt
     5881 ttcaaaaagg tttacgcgct gggtagtaac acgtaactca cgagataaaa gccgtatttg
     5941 ctcttccaaa acttctgctt caaggtcata ggcaagggcc ttttccatgc ggtcggctgc
     6001 aaggtctatc caaagcggcg taagatataa atcatagtcg ccacgggcaa aatcagctcc
     6061 ttcgtaaaca ggtatggtta ccccggcgat attggtaaag ccttttttta tattgcttac
     6121 cacgaccatt tcgggcttaa aaaaacctgt ttcaccaaag accgcaatcc acgaagaaaa
     6181 ttctttttcg agcttttctc gcgccttccg caattccttt gcacgaattt caatatcgcg
     6241 aatttcgctt tgcagttgtt gttttttcaa agtaagagtt ggcaaatagc gtgtatagcg
     6301 tttgagtgca tctttttgag tttttagttc attttttgta agctttatcg ccatatccgt
     6361 cctcttttat tgtggccaaa attttgcgat gagatcggat tttaagccag tctcttcttt
     6421 ttcaaaacaa tctgcaagga ttttccagcc taaatcaagg gcatcttcta aagcaatatt
     6481 gatagataag tccatcattt ccttttcgaa gagttctccg tattttaaga gtttttcgtc
     6541 ccattgagac atcataaagc ccatggcttt tttctcgagg gtatccttgt aagaggcgta
     6601 gaggcgaatc ataccgtcca ttaaggcgcg gtgatcttcg cgcgttttac cgtttacgtt
     6661 ttgtttaagg cgtgaaaggc ttccaaaagg ctctatacga ccacctttga ggtagtactg
     6721 tccttcggta atatagccag tattatctgg cacaggatga gttacgtcat cgccaggcat
     6781 agtggtaacc gcaagaatgg taacagagcc tgcatcgtca aagtcaaccg ctttttcata
     6841 acgggcggca agctgactgt ataagtctcc tggataccca cggtttgaag gaacttgctc
     6901 ttgggtaatc gcaatttctt tcatagagtc ggcaaagttt gtcatatcgg tgaggaggac
     6961 gaggacgtct tttccttgaa gtgcaaattg ctcagcaacc gccaagctca tatcgggaac
     7021 cataaggcat tctaccgttg ggtctgatgc ggtatgcaca aacataagcg ttttgctcaa
     7081 agaacctgct tcttcaaggg tatttttaaa gtacagatag tcgtcgtatt ttaagcccat
     7141 tccaccaagg atgataatat caacctcagc ttgcatagca attcgtgcga gcagttcgtt
     7201 atagggctca cctgaaatcg agaaaatagg gagcttttga cttaccacga gggtattaaa
     7261 catgtcaatc ataggaatac cagttcgaat catccggttg gctataattc ttttggaagg
     7321 gtttacggag ggtcctccta tctcaacgag gttttcactc aaaagaggtc ccttatcccg
     7381 tggttcgcct gctccgttaa agatgcggcc gagtaagtcg tcagaaaaac ttacctgcat
     7441 ttcatgaccg agaaaacgga tttcgtctcc ggtagaaaca cctcgcccac ctgcaaaaac
     7501 ttgcaaggaa acaaggtcgc cgtcaatttt atttacttcc gcaagagatg cgccaaaact
     7561 tgtttgaatt tctgcaagtt cgccgtattt aatatccttt gctcgaacgg taataacaga
     7621 accatttata gattcaatct tgctatatat ttttttcatt tttatttagc tccttcaagt
     7681 aaatgcttcg ctttatctga aagttttttt accttgcttt catagagggc atcgagatct
     7741 ttttctttct ttgaaaactc ctcgcttccc caaagcacat agttccagtc aataaaactt
     7801 tgtcgtaatt gataaaaata ggttctcgca tcatctttac tgtctagatt ataatctgaa
     7861 ccgagaactt gtattatttt ttggaagcta taattttgtc gttcaacggt aacagcagaa
     7921 tctatctcgt caaaggagtt ttgttggaga tagacagcat cgaggaattc acccttgaga
     7981 tagacaatgt agtcgtcaag gctggttcct tcttcaccga caaccttcat catttgctct
     8041 atttcgttac cacggtgcat ataatagcgt gcaaaatcga cccagtcttt atctaagacg
     8101 ctcttgtact ttgaccatga ttcaaggggg tcaatcgcag gatatttacg ggcatcggat
     8161 cgctcacgag agagtccgtg gaaggagccg acaactttga gggttgcctg tgtaacgggc
     8221 tcttcaaagt ttccacccgc aggtgatact gttccgccga tggttacaga acctgttgaa
     8281 ccgtcgtgaa ggcgaacaat gcctgctctt tcataaaagg ctgcaatgta ggactcaaga
     8341 taggcgggga aggcttcttc tccaggaatt tcttccaagc gtcctgacat ttctcgaagc
     8401 gcttgcgccc atcgacttgt tgagtcagca aggaggagga cgtctaagcc catttgtcgg
     8461 tagtattcag caagcgttac accggtgtac accgaagctt cacgagcggc aacgggcata
     8521 ctggaggtat tacaaataat aatagttcgt tccatgaggg agcggcctgt gcgtgggtca
     8581 gtgagttcgg ggaattcttt taagatttct acaacttccc ctgctcgctc accacaggcg
     8641 gctacaatta caatgtccac gtcagcgttg cggctcgtgg tgtgttgtaa aaccgttttt
     8701 cctgcaccaa agggtccggg gatacagtag gtaccgccct tggcaacggg gaaaaaggtg
     8761 tctatgagac ggatttttgt ggtaagtgtg tctgtgggca taaggcgctc ggcatagcag
     8821 tcaactgaac gttttaccgg ccagttaaag ctcatactaa gattgtgttc tacaccgcga
     8881 gcgtctttta caacagcgat ggtatctttg agggcatagt cacctttttc ttttactgaa
     8941 acgacggtgt aattgcccaa aaaggtaaag ggaaccatta ttttatgttt aaaagtaccc
     9001 tctggcactt cacctataaa agaacctgcc tttaccgtat cgccagcttt tgcgatgggg
     9061 gtaaaagacc aagtgcttgc atcgggaagc gggtcaagat agacgcctcg ttcgaggaaa
     9121 tatccagctt gttctgcaag ttcaggaagc ggattttgaa gcccgtcaaa aacttgtcca
     9181 agtaagcctg gaccaagttc tatggataaa agttcattgg taaaaaagac ttcatcgcct
     9241 gtttgaatac cctttgtcat ttcaaagact tgcaattggg cttctccgtt ttgtattcgg
     9301 ataacctcac tttttagttt tttgtcccct acagaaacat agcctacttc attgaggctc
     9361 actgttccgt caaaggcgac acttataagg ttgccattta cagcaacaac tctacctttt
     9421 gtatctgtca ttttgattct ccaagaattg tatcatacat tgatcgatat tctgctaatc
     9481 cagcttcttc gttaaattgc tttatgcgca aggcaagttt taattgcaag gcgtaggcat
     9541 aaactgaatc aagactaaaa tagtccattg gttgtagttt ttttatcgct aaaaagcgtt
     9601 gctcgtttaa aaactgttca gcctcaagag gactttcaaa accagtcgcc gttctcgcta
     9661 tcgaaagaat ttcaaagctt tgagaaaggt tatttggaag agaaaagtct tttttgagcc
     9721 tttgtccgcg tatttgtccc aaaacaaaac gtagctcacg ctcccaagca tagtaggcag
     9781 tcaaaaagga ggagtcaatt tctcgtggtt ttttttctgg tacaagggag atatttttta
     9841 gtgttttaag ttgcgcctcg tttaagtttc gctcagcaag ctctaaaaaa taagactctg
     9901 taatgggcag tgggctcgat tgtgaaagag aaaaatacgg caactgcgac atgagataat
     9961 aaaaactcat tttaattacc catagtttga gaagcttctt tcattatttt tgcagtttgt
    10021 gggtttaagt atgaggaaaa aagacttgca atagattcac tcgagaagtc ataaaaagct
    10081 tcgccgtctt ttaagctcaa gcgaaaacca tcaactactt cttcggacat ttgtatgcgt
    10141 aagccttttg caagctcagc tttgagagat gccgttaagg atgattgcac agagtcgagg
    10201 tcttttttat tcaataaaac agagacatct ttcgcatctt ctttttcagt ccagttttta
    10261 atcacctctg gaataagtga tttaagtaaa tctgcagaaa gtgcagactg cgccgcatct
    10321 tttaaaagtg catttaattc ttttacaaca ttttcacgga atgttaacaa aacatttcga
    10381 cttgcttgtg cgattgcatc gacacttgct ttttcaagac gctctgcctc aagctttgct
    10441 ttttcaatgt ttcgtgcagc atttttttct gcctcttgca cgatgccttg tgcagatttt
    10501 tctgcttctg aaataatttt ggcagcttct tcttctgcag tcgctacccc gtcaagctta
    10561 attttgtcaa ttaattcttg taattggata tccataagac ccctctatga ttattgtgag
    10621 aaaaatctca cgtatttaca atagtatcga ttatagaccg atacaattaa aaatgcaata
    10681 caaaaacaca ttgattgtcg agcaatattt ataagcagtt ttttttatca agctacttga
    10741 gagctttata aagatggctt atactctaag aatgaaaaaa ggcacaaaat taatagcgca
    10801 aaataaaaaa gcgcgcttta attatcatat agaagaagat tatgaatgcg gcattgaact
    10861 caagggtaca gaggtgaagt cagtgaaaaa tggctcccta tcctttcccg atggctttgc
    10921 agaaataatc gataacgagg tatggctaaa aaacttctcc atagcagaat atcccttttc
    10981 aaagcatttt acccacgagc cacaaagacc aaaaaaactg ctcttacata gggctgaaat
    11041 caagcgctta aagaaaaaaa ccgatgaaaa aggatacaca ctcataccgc tcgatgtata
    11101 tctcaaaaac ggtcttgtca aagttcgcct tggcctttgt aaggggaaaa agttatttga
    11161 caaaagagat acgatacggc aaaatgattt acatcgagaa gtacagcgtg aatttagaaa
    11221 gaatcttgag ggttaaacga aaatatgaaa aaaagccttg ttataacaat aacaattttt
    11281 cttttacttc cttgtgtgtt ttcacaaaca aggacaaaag aacttggctt ttttgctccc
    11341 atttacaata cgaaggatga aggaacagca aagctcatag tagacttata ctataatcat
    11401 cttttaagct ttgatagctt tcgcgtacag gattttcgct ccattgtttt ttcaaaagac
    11461 aatattctag aaaactatgg aattcaatta gcatttttta tagatatttc agatagtgat
    11521 aataactgga caatagcttt taatactttt tttgacaata aaacacatac tgaagaaaaa
    11581 atctatgaca gctactatca agtccttctt tctgcgaaaa atgatttatt tgttttttta
    11641 gatagactcg aaaaaaccga ggaaaataca gcaagagaaa aatctgacga tgtctcagct
    11701 acaacggtta gtatcaattt agattccatt gcaggaacct ggtttactga tgcttccatt
    11761 gacaaaatta ttattttacg tggtggaagg ggttttgtta tttataaaaa tggtgcttca
    11821 atgaatatat cagttgaaat tcgaggaaat caaattattg ccctgcaaac aagtaagaca
    11881 aatgcttcct tttttcccga actacctcgc gaccttgcct tgaaagttgc gacagaagaa
    11941 ctgcaaccca taaaatggat aatgacggca aaaaatgaaa acgaaatggt tggagagaaa
    12001 aatcattatg aagcagtcta tgaaaatgat aaggcaacat ctgttaaaac ggtcagtagc
    12061 ccatcaaggt ggacacgata atgataaaat ttaatcccca aaatcccctc gatgccttag
    12121 aagcttggct tgatacctac ttgaactttg aaaaaatgcc taaaaaagat tttttttggc
    12181 ttgatacaat gcaattttta tgtaaaaaat gcaataatcc acagaatacc tttcaaagtt
    12241 ttcatgttgc tggctccaag ggcaagggct ctgtttcaac ctttattgca tctattttaa
    12301 ccgaggcggc tatcgattgc ggtttgtaca cctcccctca tctttgtgct ttttcagaac
    12361 gcgttacaaa atctcatgct gtcttagatg actctatcta cgcaaatgcg tataaagaat
    12421 tgagctatac aattgactca attatcccca aaacctatcc aaaccaacgt gaaccctcgt
    12481 ggtttgaact tgcgacgctt ttcgccttta tcagctttaa agaggctggt tttccttggg
    12541 ctgttttgga aacaggtctc ggcggaaggc tcgatgcaac aaatgtcata ctgcccgctg
    12601 cttctgttat taccgaaatt gagcttgaac atcaagaata tcttggcaac acagtcgaag
    12661 cgattgcccg tgaaaaagct ggtattataa aaaaaggtat accggttttt tgttcttcca
    12721 cagaagcaat tgcttgtgcg gtatttaaga cagaggcgaa gaaaaaagac gcagactatt
    12781 ttcatctcga tgattttatt gacaaagtac aatactcgta ccatgaagac aaaatgaaga
    12841 tagaagcatc tatgccagag cttttttcac gccctttaaa agcctccttg caacttttag
    12901 gagcatatca agtaagaaat gcccttcttg caagcctcgt cgtgaaaaaa atcctccccc
    12961 agatttcaca aaggcagata gaaactggct tagaaaatgc ttttattccc ggccgttttc
    13021 agataataaa aaaaacaaag caaaatccac tcatcgttgc agacggcgca catacaaaaa
    13081 aaagtatttc ccactgtctt tctaccctac aagaactttt tctgtcagca ccgaaaaaag
    13141 actctagcaa acaacgtgta gagcctcgtt tttcacttct ttttgcctgc gctatggata
    13201 aagatgtgga agcaattgcc catttactcg tagaatcaaa gctttttacg cgcatttttc
    13261 taacaaaacc tggcgctgta aagcaaacaa atcttgaaaa agccaacgaa gcctttgaaa
    13321 aagcagtcaa tgcagaaatg cttcaaggat cacgttacgg ctcctcaaaa aacagctctg
    13381 aaaaccaaat agaaaaaagc gaagtaaaac cagcattttt tctcatagaa tcgtatgaag
    13441 aagccatgga aaaagccttt caagaagcgc taaaacagga cgaaacactc gtcgttacag
    13501 gctcttttta ccttgtttca gaattgcatt cttttcttgc aaactcgata agatcttctt
    13561 caaacagtgc ataagataca gttttgccgg ttttcggatc tataaaagaa agaccagatg
    13621 caaaaaaaag cattggcttt tgcgattttt tcgggttttt atgcgtttct cctattctat
    13681 ctgagtcaga ttctcttcca tatcgcatgt cagctattat cggatagcca agccagtgca
    13741 aatggctccg tacttgatgg cggtaggcaa gacttagaga aactcctatc ttccttgcaa
    13801 aaccgagatg actgagtgca tcgatgctcg tctgatatat ttttttatcc tttgcgtttt
    13861 ctatcaaaag agggcgaact gatgctcctc gtggaccaaa attgcgaaaa gcggatgcta
    13921 tcttcaaagg aagctgcata gtttcttcaa gacgatattc tcctggcaag ggaggataac
    13981 ccctttttag gctttttttt acatcctttt gagaaatttt gtgtgaaaat gcggtatagc
    14041 atttacgaaa ggaagttttt ttttgtatat ctataaaaaa atcgtagctt ttttgatttt
    14101 ttgcaagcaa aagtaagcct gatgtggcag tatcgattct atgcacaagt ccgccttcaa
    14161 taggcttaaa ctgagatgct acttcaagca cttctgggta gatttttgct acttcacgta
    14221 aagctgaatc ttcttcccct tcttcaagga aatccttcga caaatcctcg ctatttattt
    14281 tttttaaagg agccgaggct aaacctgctg ctttgtaata aataagatag tcttcagcgt
    14341 caaaaaaaat ctgagatttt ttcaaatcga tgctttttga aaattccttt tgagctattt
    14401 ttccttgggc attgacgagc attttattct ccgttttttt aaatctacct tagcttttgt
    14461 gttttttcat ccacattgaa ttgcgctcat attctttatg aaaaacctgc aagacttttt
    14521 taaatcgaat aggagtcgct gctttaaaaa agctgcgctg tttttgctca ggtaggcgaa
    14581 tgcccagctt agaagcatgg agcatgaggg tcgctgtttt aaagcgagaa tctcgaacac
    14641 cgtacaatgg atcccctaaa ataggacaat tgatatattt catatgtacg cgaatttgat
    14701 gggttctgcc tgtttttata cgtattttca ttaaggcata aggaccgtat aaagctatac
    14761 aaatataatc ggtatgggca aactttccct gactctcgtc agtgctcgct ataaattttt
    14821 gtggattatt tggatcacgc attatctggg tttttatagt ccccttttca aaaggagggt
    14881 gtccctttgt tatagcaata tactgcttta taacagaatg ctttttaaat tgctttttta
    14941 aataggcctc agttttcgtg ttttttgccg tgataataag ccctgaagtg tccttgtcca
    15001 aacggtgtac aattccgggc cttttttgtg cctcaggcca gtcaagtaag ttcgcttctt
    15061 tttggattac ttccctaccc cagtgaaata agagggcatg tacaagcgtt cccgaccagt
    15121 ttccgtgcgc aggatgtaca accatgcctt gcgccttgtt caaaaccatg agattttcat
    15181 cttcataaac gatatttaaa tcgatttcct ctggcttaat agtacttgga atgggatttt
    15241 tccattgtaa gtgaatagta tcaccatttt ttactcgact agataacttt gcgtttttat
    15301 tgttacaggt taaaagcact aagccttctt ttaagcgtga acgagaaaaa gatggatcca
    15361 attgtgttat gagatagaga tcaagccgta atctttcgcc ctcagccaag gaaaccttcg
    15421 attctatgtc tatcatttat tcgtcagcct ctatgacaat gcgtttaatg gtatacggtt
    15481 ctgaagtttt cactttttct tctttcactt tattttgttt tatcatgcta atgctaaaat
    15541 ctataatgcc caccagtaaa ctcgctgaaa gggagataaa aagtacttgc ttttgttctt
    15601 ctgtgctcaa atttgcttga gaaggagaaa atgggttagg aaaatacgct gaatcaaaat
    15661 catttgctgc atatcgataa aaggaatagc ccaaggaagt ggtaagcgtt gtaaaaggaa
    15721 gtgagccaag cgttacaatt tcgcttcgcc tcagttgttt catccaaggt ttaaactcgt
    15781 ccttcgcata gggctctggg ctattatcag cccacaaaag agaaaaggaa aacgatatga
    15841 taagaaaaaa aacaagagtc tttttcatag gcttagtata tcatatacat agcgcattgc
    15901 ctatgcaaaa aatgagggga taaataaggc tgaaccaatt ctaagcatag tcgccccttc
    15961 ttcgaccgca attgcatagt catttgtcat acccatagaa aggacatcca aatgcaggtt
    16021 tgggtagcgt ttttttgctt cttcttgaac cgagcgcaat ttttgaaaag aagaacggat
    16081 tagtttttca tcttttgtaa aggcagccat agtcataaaa ccagcaggct gtgcaagcaa
    16141 tcgaggctca ttttcggaaa ggagataatc taaacaaagc caaagttcat cgaggtcttt
    16201 aaagccgttt tttgaatcct cagcagtatg tatttcaaaa agcagctcga tgggctggga
    16261 atcagatgcg ctcacttcat ttcgtttttt tacttctttt ataatctctt ttataagctc
    16321 gattctatcg actgattgta tacaagtagc aatcggcaaa agatgtttta ccttatttcg
    16381 ctgtaagctt cctatcatct gcaattttac ttggggattt ttttctaaaa tcgatgggaa
    16441 tttttctatc gcttcttgca ccctgttttc tccaaaaagc tctaagccca cattgatagc
    16501 ggcttgaatt tcttcgatgc tgcgaaactt agataccgca agaatcgaaa cctcactcga
    16561 ctttcttgcg ctttttaggg cagctttttc tatattttct aaaatttcat ttaaggcatt
    16621 ttttatattt ttttcgtata ttgtgatatc actttctttt ttatcattca ttttattttc
    16681 cacctttttt ttcttgtaaa aactgataac tgcaatcact caaataaaga atattttcaa
    16741 ggctcaggtt ttcagcccgt tcatttgctt tgatgttcgc attctccaaa acagtttctg
    16801 caatgtcatc atttttataa aactgtatga gattattttt tattgttttt cgacgtgaaa
    16861 gaaataaggc tctttgcaat tccataaaat gcccggcatc gaggcattgg gggaaatctt
    16921 cttttttttc aagcaaaagg ctgcgtgaat caacgtttgg acgaggccaa aaatgagaag
    16981 gaccaaggtc tataattttt tttacattgt aggcccattg tattaaaaca gaaaaagaag
    17041 aatagtcttt ttgtcccggt tttgcatcga ttctttctgc caactctttt tgcacagtga
    17101 taatcgcttt ttcaaaacgc aaacctttag aaatagtgtt tgcgagtatt tgtgaagaaa
    17161 tattgtatgg taggtttcca aaaaaataat ctggggctcc ctgcgtttcc cattcgtttt
    17221 cccatgtttt tagaaaatct ccttcgacga gccgaaaaga tgcaggtgca agtattcttt
    17281 cgggtgaaaa tgccttacgt aaaacactgg aaaatccctt atctatttca aaggctataa
    17341 gtgtagcttt ttcttccaaa agatgctgcg tcatcgcacc taaaccaggg ccaacttccc
    17401 atattttttt cccttccaca tttccaaagg cagcaagaat tttttttcga gaactttctt
    17461 gtatgagaaa gttttgccca aacttttttt gcattgccat ttcatggctc tccaaaaaca
    17521 attttatagc tgtgggagaa ttaaaatcgg gtaaaaaagt catacacctt cctttttttg
    17581 aataagtcta ttttgcacaa gaataaaaaa ctaaaattgt attgcaggca cctttgcaaa
    17641 taatactacc agaaaagcaa taaaagaata aagcccctga ataagaacgc cactaaaaaa
    17701 aagcgtttga ggtaggcaaa gtgccaaaat aattgccgtt atggaaagac tcataaaaag
    17761 agaaataagc gggcttataa aaacagttgc gataatgcct atcggtgtaa tagtaccaaa
    17821 aaatgcaagg gtcaagggta tggtaaaaat ttgtgcggca agagaggcag aaagcgaaag
    17881 agcaatcggt tctgggaaaa agcgaataca ctttgaaaag aaaaaacggg agaacagggc
    17941 tattcccaaa agcgcagaat acgaaagcaa aaaagagaga ctcatgccat catctggtgc
    18001 tatgagaatt tgtagtataa acgaaatcga taaaaccgat gtaagattta tatcgaggga
    18061 ggcaaaagaa gaaaggagcc cgataagggc acaaatatag gcgcgcatta aagatggcga
    18121 aaatcctgca aaccagacaa aaaaacttat tgccaagaag gaaaaaaaag gagcgcgttt
    18181 ttttccaaaa atcctactgg caaaaccagc agtaaggcct gcaaaaaaag aaagatgcat
    18241 gccagaaagg gctaagatat gagataagcc agcatttcga aaggcatctg ccaatcgtgg
    18301 ttctaagtac aatcgttccc cagataaaag tgccaaaact aaactaccgc cttcacccca
    18361 ggtatacaaa agccgcttaa aagctaatcg caatagcgcc cgaaactgtg aaagccctga
    18421 tttccagccc ctttgttcag ctttttttat aaaaaatgtt ggtaccgcag tttttttagt
    18481 attcatactt tttttcgtaa aggagccaga acaatcgagg atggagcccg attcaaaaac
    18541 cgccttcgag ccagtagagg aaaaaagcct tccaggatac aaagattcaa ccaaagcagc
    18601 gggaacaagc gcttctagcc tcccctctcc ctcgtgtgaa aaagtgaggt tttgtctctg
    18661 catgccaact tccaaaactt gggcgcgaag tagaaagtac tgctcattta ctttttgtgg
    18721 actttccaaa agacaaagtg aaagcctgtc gattgtgtcg ataggaaaaa cagtataaaa
    18781 gggttttcgc actggaatag aggcataaaa aagataatac ataagaataa ctacaagggc
    18841 aagtttttct ataagacttg tatttttagc actaccaatt aagctttgcc aaagcctcct
    18901 tagatgcact gataattgag tcaggatagc cttcatgtaa gataacatag agtaagttgt
    18961 caaaagcggt tttttctccc agttgtgcaa gcgctgatat taaatggagc atgatttctt
    19021 catcaaataa tccggtcttt tcagtttttt tgtttaaaac tgccagataa tctgacaaag
    19081 cccttcctgt ttcacttgtt gcaagcaagg ttaacgagtc cataacttcc aaaagctcat
    19141 ccttacttat aagaaaagca gtatactgtt caaccgcaat cggaaaatag cctgcaacaa
    19201 ggctcgcagc tcgtgtccat ttcgcttctt tgataatacg caccgattcc atttgaacct
    19261 catagaattc ttttaaaagg ctctcacgtt cagtacctgt attatcacct atacttatta
    19321 ttgtcgctct taagaggttt tctgtaactt ccgctttaaa aaataaattt aaatggggat
    19381 ttcgacctaa aatgcttaaa ataagctttt tttcttgcgc tgtcgttgtt gaaatcctct
    19441 ctagtaaatc atttttatag ttttctatag aaagagctaa aacttcttca atattctttt
    19501 tcaattcaaa atcaatattt ttactcaata agggataaat tttattaaaa aaggcaaggt
    19561 cttgaatcga agaaagatac tgcaaactgg caaatattga tttgtttgat cgcctgctat
    19621 agtctttttc tatttcttca aagagaaaat tatagagatt ttctgtaaaa aaagcctcac
    19681 ttatagcaat ttttgttaaa aggtttaaga tttcaacgcg aatttcttcc ttgttgtaga
    19741 gaaaaaacaa ttttttaagt gccgagtcat agggctcaag attatcttga ggaagagcat
    19801 ataaactgac aaaaagtaag tcatggagat gcgtagaatc ggataaaata gcagcatttg
    19861 tcaagcaaaa gtccaaagcc atatcgagta agggtaaatc tttttctagg attgctattt
    19921 gtttttttag aatagaaacc ttatcttgta tactgctgct cacaaaggct ttttgctcct
    19981 gtgaaaaatc tgcaaataaa aaaacagttg ataaaaagaa aagtatagta aaatatagtt
    20041 ttttcattcc atttctcctt gaaagaatga ttcagtaggc atatccatag tttctccaag
    20101 ttcagtatca ccaaagtcaa tctctttatc cagcccttcc gcctcttcac taacttggtt
    20161 ttcatcttct tctacaaggt tcattacatc taaaacatct ttttcttctt gaggtaacac
    20221 attatataca tagcttaaaa tagtgttttt catagaagga tttattaagg ttgcttcaaa
    20281 atgcagtaag gattgattta gtttcgcatc atattttaca tttctcacga gtccagccat
    20341 aataacgagt gaatcattta attggaattg taatttaatt tccatatcct ttaaaccctc
    20401 tccaccgata cgaataagcg ctcctgtttc tgaaatatct tcaagcatac attttaagcc
    20461 ttggctatgc tccatcgcaa aaggatcact tacagcgagg ttaacaataa agatctgcgc
    20521 gggaatctta caaggcacac gaatggattt tctattttgt gtgcgcataa gcgccgttgt
    20581 atgcgccagg tataaaacat tttgcccatt atacaaagag ctatccaaaa ctgcagtgtc
    20641 aaatacatag gcagcatcat tttttctcca taaatatacc gatatatctt tcccaatcca
    20701 atctaaacca gtaattgaaa tagtcttgtc ttttttgggc acttcgataa tgagatttct
    20761 tccatttgca aggatttttg aagtaaaaac acctttacct gttaaaataa ttcgtaacac
    20821 ctgtcctaca tcgagctctt ttgtgctttc taagcctttt tttcccaaac ttaattctac
    20881 ttcagtccga aactcataga gtttcgttaa aaaggcatta aaggagtctg ttttatcctg
    20941 ccccttcgct cgggcaatcg ttaaaagttg gctaatcgtt ctatcaagaa ccggtataga
    21001 aaaataaagc gatgtgggat cttctaagtt tgcatttttt gcaagtgtcc ataacatatg
    21061 tatttgacga aatttaaagg aagagtcaaa acccatagag aaaaaacgaa tcatatcttt
    21121 aaaatgtctt aagattgcca cgatcagaag aattattaca ataaaaatga taataataat
    21181 aaggttatac acattattct cctagagtag tataataaca cataagctat gaaaattcag
    21241 catttttttt atcaaataag tgaattcttt attattctca ttttatttgt tttaccaagt
    21301 attttttcaa aagaaatgac gacttttaca aataccaaag aatttttctc ttttgttatc
    21361 tatcttttct tctcaatttt cctccttttt atactcaata aaaagaataa aacagaaaaa
    21421 aaaacacaaa aaaagcccat ccttgtctct attgtaagcc ttatcttgct cttatggctt
    21481 tcttttattt tttctgtatc cctaccagtt caagaaaaaa gctatataaa accaagcctt
    21541 tttgtgcaaa atcttttttt tatccctatt ttggtaagct ttgaagaagt tttatttcga
    21601 gcctatattc ctgacaaagt tttttatctt tttacaataa aagcaaaaaa acaaagtcgt
    21661 tttataaaac ttcttgcaat agttttttct tctctccttt ttgccctcgc acattcctac
    21721 gcaggtttct attcgattat ttttgccttt ttaagttccc tcatactttt tgttttaaaa
    21781 gactatacta gctctctttt ttttcccatt cttgtgcata gcctttataa ttattttgct
    21841 tttttctatc tcttaaaaac atagacaatt taataatttt gtcctataat caatttgata
    21901 aaaaaataag agggacggta tgacgcttta tatacttgct ttaattatag cctttggtat
    21961 ttctactatt tccatgccta taatcataaa aatatcaaaa aaacgaaact tatttgatag
    22021 aatagatgat agaaaagtgc acacagggca aatttcccgt ctcggcggca taggtataac
    22081 aatcggtttt tcaattgcac tcgcaatcct ctattttagt ggtctttttt ctacaataca
    22141 gcaaaatatt tggtatctac tcggagcggg gctttgtatc tcagcaatgg gaataatcga
    22201 tgacttaaaa gcgctttctg caaagttaaa actgctgatt caagtcgtag cagccatact
    22261 cgtactcatc ggtggcttta aattcactgg aatcgctata agttctgtct tttatctaaa
    22321 tttttctttt ttttcataca taattacttt ttgttggatt attggcgtta caaatgcgta
    22381 caatttaata gaaggtattg atgggctctg tggtggtatt gcaagttttg cttccttgac
    22441 ctttgctttt atttttttta agtctgacaa tacagcagct atttttattt gcttatcact
    22501 tgcaagtgcg atactcggct ttttgctgtt taactggccc ttacccaagg caaaaatttt
    22561 tatgggagat ggaggtagtc agtttttggg ttttgttctt gcagtccttc ctctacttag
    22621 cagcaagggc tccatgccaa ccatatccct gccctatgct attgcggtct tgatgatacc
    22681 catttttgac accattgctg ctatttggag aaggctccgt gaaaaaaagc gcattgacag
    22741 tcccgataga tttcatatcc atcataaact tatgctactc ggcttttctt ctcgttcgac
    22801 aatggttata ttgtacctat tacaagcaat tatttgcata tttttagctt cagcatactg
    22861 gcttggcggc tatatctctg tatttatcct tcttgcagat tatcttaccg gagttttatt
    22921 tttcactata atacatatag gcaaaaatca agcccttaaa acagatacgc aggaagcata
    22981 agagagcctt ggcgaattag ttttacagaa tgctcttcta tagaaacaat cgtagaagct
    23041 aagcctgagc cactcgaagg atcttcaaca attaaagcga cttctttgga aaattctttt
    23101 tcaatttcat caatatcgct taatggcggc aagccgcttc tattacaact tgtagaatac
    23161 agtggacttt ttagatccgc aataagtttt cgtagccacg catcccctgg acagcgaagt
    23221 gcaatgcttt cttcgctatt tcgtaaggga agaataattg ttagagcacc cggccataag
    23281 gcaagcaaat cctgatgtgg gcgaatatca ctaaacagag aaatatcttc tggtttttct
    23341 atgagcacta taaaaggttt attttcttct cgccccttta tagcctggat tttttccttt
    23401 gttgtgttta tttttcccga aaaaccataa attgtatcgg ttggtaaaac aataaccttg
    23461 tcattttcca aagctctaag aatagaaata taatttttac tatcgttttt aagaatacgc
    23521 atgcaaaaac ctttcctgtt tgtttgaaaa cgatttgttt ttaagagaaa acaaaaacaa
    23581 aacaaggtag cttaaaataa aaatgacaat accacatgtc ttgcttatcc gctcttctac
    23641 aaaaaagatt tcagttttaa ggtatttttt acctatttcg tgctgttttg ctttattgct
    23701 tggaatccca gaaattttga gcaaagtttc accttctgag atatagccaa tctgttttgc
    23761 ataggaggct ataacctcag tatcttcagt caatgccttt tgttttaaaa taagactatc
    23821 ctgtgttttt tgaattgtat ttatatttgc cactaacatg tctttttgtt ctattagctg
    23881 cttttctgcc aaaacaccgc tttcaccaaa tacaaaagat atacccacat agcagagcgt
    23941 tcccaaggct atggaaatta aaatgcgttt tattctcata ttatatctaa cggcatattt
    24001 ttttgttttc ttaaaaaaaa atatgtgtta tactagttct acatatttat aagtataagt
    24061 gtttttttta gaaaaacagc cgatactttt aaagaataat aaaactatct atttatggag
    24121 taagatatgt ctttggaaga aaataataag attgaccaat atggtgtgtg gctaaaaaaa
    24181 acaccgcttt tagatgaaaa agaagaacaa acagaaatag aagttgaaga tatgtctgaa
    24241 aacatgtcgg atgattttat tccaatcgac gatagctttg acacgggaaa aaatgcagag
    24301 gttgctgaat cagcctccct cgatgttact gaagaaaaca tacaagatga ttattcagac
    24361 tttattgaac aaagcaaaga agatattttt gatgaggaaa tgtctagtct tttagaaaca
    24421 gaaacctcag tagatcaaga aatattagac actgcccctg aagatgtatc cttagaaaca
    24481 ttttcagacg gagaaattga tctttcagcc tttatggatg agagcgaaaa agaaaaagac
    24541 aatgcagaac catcaaaaac agaagaagtt tctctcgacg atttttcaga tggagaaata
    24601 gacctttctg cttttatgga taatgactca atagattctt cttctaaatc cacgagtgag
    24661 gaaataaata tagacgattt ttttagtggc acagaatctt tacatgtaga cagtttttta
    24721 acgaatgaag cgccaaaaga agatattttt gaagaagatc ccatagatat agaccttgtc
    24781 tttgatgatg cttttgcaag tgaagctata tcaaatcctt ctgaagacat agaaattttt
    24841 tatacgtctg attcagacgc agagtttgaa tcatttaatg ttgacgacct attcaatgac
    24901 acagaagaat tatttgaaac agaaaaaatc tctcctcaaa cagttactta tgaagatgca
    24961 aatgaatttg atgacttact gtcttctctc gatgaatcaa gcccgtcagt tatagataca
    25021 gaagttacta aagctaaaaa agcaacgcaa gtgtataata ttagcgtacg tgttgacgat
    25081 gatttcgaag ataaagaaaa cataatggaa aaaccaacag aaaaagaaaa catagaaaat
    25141 aaaagagacg aaaccttgga cgatataccc ctctttttga ataatcaaaa attcaccaca
    25201 aaaaaagacc ttgaaaaaaa cactagcgaa gagtatacta atctcacgag gttaaataag
    25261 gaagcaagca tggaatttga tgaattagat gacattttag aaaatgtaga aataatcgat
    25321 ggaacagaag atacctcaac aaaaaaaaat ctatctgagg aaactgaatt agtaatcgta
    25381 aatgaaacaa accctttaga tgaagaagaa aacctcgatt taggcgaaaa aagcactata
    25441 gatgcggact taaatatgga agaagatgat actattctag agaatgctga aactgaagtg
    25501 gcaatggaag atagcactga tacttctatc gatactgata ttgaacttga tgaaacaatc
    25561 caattcaatt tagatgaaga cagacaagaa ataaacgaga ctgaaatttt ttttgaagat
    25621 gaagacgatg aaatatccat agaaatagat atgccagaag atgactcaag tgaagctata
    25681 tccacggata tagaagatgc actgtatgaa gaaaaaacag aaatagaagt agacaataag
    25741 ctcgacaacg acaatctaga ttcaatcaat gaaattgata atttagctag tatgagcgaa
    25801 gattttgatg actccctcga cacgatggaa a
//
LOCUS       DAOSSW010000015        25436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_2133_length_25436_cov_7.712817, whole genome shotgun sequence.
ACCESSION   DAOSSW010000015 DAOSSW010000000
VERSION     DAOSSW010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 25436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25436
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2133_length_25436_cov_7.712817"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1919)
                     /locus_tag="PKW26_02520"
     CDS             complement(<1..1919)
                     /locus_tag="PKW26_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOQ92474.1"
                     /translation="MASFISLVLGRSGSKKGRLILSGIVSLVALGFKIALKDNHENGY
                     HLITIILMILATGISGLPIMARAISSLRYKIVAIDLLVTIAIIGAIFIGEYWEAAAVS
                     FLFMLGEWLEARTIEKTRSSIKSLLDLAPVTARVKRNGAELEIPAEEVVIGDVIIIKP
                     GEKIPADGKVIEGFASVNQAAITGESMPLEKELGSSVYSGTIVESGYIIIEAVKVGED
                     TTFAGILRLVEEAQDKKAKTQKFLERFSRYYTPGIILLSIAVYLITRDFRLALTLLVI
                     SCPGALVISAPVSIVAGIGNGAKHGVLVKGGEVMEKLGTVKAIAFDKTGTLTKGRAEV
                     QKIQTTSALSETEILEIAAIGESFSEHPLAKAVLRMADAKLKKTDAALQLDSPENPEM
                     IIGQGIVFGHKNTKYRIGNRSLFTSLKTQDGSNLEELLSNEESEGRTCIILGTENEIL
                     GFLSIADEIRPEAKQIIRDLKKLGLKKIIMLTGDNEKTAQAIAKNLGLSAVHAELLPA
                     DKVDALEKIKKDYPRVAMVGDGVNDAPALAASFVGIAMGEEGSDVAMETADIVLMTSD
                     LQKLRYAMGLSRATVKNMRQNISFALAVAAFLLAGVLIKTVNLSLGMLVHEASVILVI
                     VNAIRLLAYKERKSFL"
     gene            complement(2006..2221)
                     /locus_tag="PKW26_02525"
     CDS             complement(2006..2221)
                     /locus_tag="PKW26_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOQ92475.1"
                     /translation="MTTKTYQLETLSCPSCVAKIEGMLKKTKGISESEVLFNTSRVRA
                     SFDEAEVNSDQIKKNIADLGFVVLNEK"
     gene            complement(2495..3832)
                     /locus_tag="PKW26_02530"
     CDS             complement(2495..3832)
                     /locus_tag="PKW26_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOQ92476.1"
                     /translation="MSRKKVLIMGAAGRDFHNFNTYYRNNPEYEVVAFTATQIPDIDG
                     RKYPAELAGADLYPNGIPIYNESELARLITELAVQEVVFAYSDVTYQYIMNRSAMVNA
                     LGADFILMGYDRTKIKSTKPVISICAVRTGCGKSQTTRFIYDIFKKMGKKVVAIRHPM
                     PYGDLVAQKVQRFAELSDLEKHKCTIEEMEEYEPHIIAGNVIYSGVDYEAIIRAAEKD
                     ADGEPDVILWDGGNNDFPFYDSDLKIVIVDPLRPGHEVSYYPGEVNLCMADIIVINKM
                     DSAKPEDIATVKANCQRLNPKALIIEADSALTVDGDIKGKKVLIVEDGPTLTHGEMKI
                     GAASVAAERFGVAEIVDPKPHAVGKIASTYEKYPNIGKGILPAMGYSDAQKKDLETTI
                     NKIEADLVLSGTPIDLGRVVKVNKPIINVAYELAPRGDGAKLLEERLVKKLFG"
     gene            complement(3943..4302)
                     /locus_tag="PKW26_02535"
     CDS             complement(3943..4302)
                     /locus_tag="PKW26_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOQ92477.1"
                     /translation="MNVLIIGASNKKERYAYMAMERLEKAGHRVFLFNPKLDEIEGRP
                     VIQSLSKIDQPIDTITLYLGPQNLEAYVDEILALKPRRIISNPGTENTFLPEKAAAAG
                     IVYLEACTLVMLSTKQF"
     gene            complement(4379..5395)
                     /locus_tag="PKW26_02540"
     CDS             complement(4379..5395)
                     /locus_tag="PKW26_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ92478.1"
                     /translation="MNTLLLAYDSCRLCPHDCKVNRNKGELGICGESAELRLAFAGLH
                     FGEEPLITGSGGSGTIFVSGCNLGCAFCQNFQISQEKMGSVVSTEDFATICLKLEKEG
                     AENINIVTGSHAIPALALGLKEAKKKGLSIPVCWNSSAYENVEALELLKNLVDIWLPD
                     LKTLDDAFSSQAFRAKNYPEYAKKAIYWMMEKSKIKIQNSTIQKKDSILEFESLTLPK
                     MLQGLIVRHLILPNRLEDSFSVIQWFKENIDTKGYLSLMSQYTPIRGREKKIDAIKLM
                     ENRFLEEYELETAFDILDYFEIENGFYQELNPSDDWLPDFNQTQTFPNELAKPLWHWK
                     YGFL"
     gene            complement(5469..6656)
                     /locus_tag="PKW26_02545"
     CDS             complement(5469..6656)
                     /locus_tag="PKW26_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HOQ92479.1"
                     /translation="MENNDTKIILEAKKVSKLYGLNKGDAIKLLQKGATKEEVLKKTG
                     VSVALWDASFDVKEGEIFVLIGLSGSGKSTMVRCLNRLNKPSSGEIFFKGKKIQSFTK
                     KELQDYRRNHIAMVFQSFGLMSHRDVLSNVVYGLEIKGVGKEEREEKAQEIIEMVGLS
                     GFEHHPITSLSGGMSQRVGIARALATDPEILLMDEPFSALDPLVRKEMQFELLSIQKK
                     LKKTVVFITHDINEAFKLGDRVGIMKDGKIVQIDSPEEMSTHPADSYVEDFIEGADKS
                     QVLTVKNVMIKPRSLVKERDSLKTALRQMRSNDVSSVYCVDNDMHFLGIVLINDVIQA
                     MQENKALKEIIISDIETTSEDTAISEIIPIASETRYPIAVLDEEKKLKGLVSKASILS
                     SVH"
     gene            complement(6643..7509)
                     /locus_tag="PKW26_02550"
     CDS             complement(6643..7509)
                     /locus_tag="PKW26_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOQ92480.1"
                     /translation="MKWLIDFPFEITINKKAIDSAVRSFSVKHDAFFSAITSGLNGLI
                     GGIHSLLNIIPWWLFILAIFFAGWKSTGKLKNGIFFALAITVIGIAGLWALMLETLSI
                     IIAAVIISLFFGLPLGILLAQFDRAEKIARPVLDTMQTMPVFVYLIPALLFFGLGKAP
                     AVIATTIYAIVPVIRLTSLGIRQVDKEVVEAAKAFGSTGVQTLIKVQIPQAKATIMTG
                     VNQTLMMAMAMVVTCSMIGASGLGMEVLMGVQRIEIGRGLIAGIAVVILAIVLDRITQ
                     GIAEKGQTNGKQ"
     gene            complement(7585..8583)
                     /locus_tag="PKW26_02555"
     CDS             complement(7585..8583)
                     /locus_tag="PKW26_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ92481.1"
                     /translation="MKSITKGSLIFIGLILTLSFFIGCTTEEKKTIIFADPGWDSVQL
                     HNEVAGFIAIHAYGYDSWQQITGTTPITHEGTIKGEIDVHLEEWTDNIASYTADRDAG
                     KLKEMGVNFDDNMQGFYVPRYVIEGDASRGIEASAPNLKTVQDLALYPDVFQDPENKT
                     KGRIYGSIPGWEIDTIMFNKYEYYGLDENFIYFRPGSDAALAAAFTSAIDKGQAIVGY
                     YWEPTWLMGLYDLVLLEDAPYIDEESYMAGKTECPAVVCTTAVSNAFYEKDPAFCNEF
                     VANYKTSSALISEALAYMSNNNASAAETALWLMKTYDNLLDLWLPEDKAALVRQAL"
     gene            complement(8678..9295)
                     /locus_tag="PKW26_02560"
     CDS             complement(8678..9295)
                     /locus_tag="PKW26_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOQ92482.1"
                     /translation="MATIKMASYQKIALSIAQDIVDGKYAVGEKLSGRSTLASRYKVS
                     SETIRKAMAVLQDVQVVSIKPSSGICIQSALKAKAFLEQYTSAHSIKELRNDLLEWMQ
                     RQKQETDAMRSTMRKLLDMTERQEAISPFTPFTFKLLASMNHIGQTSSDLQFWQVTGA
                     TIIAILRGDEMLLSPGPYAEFLEGDLLYFIGPKESYERVKNLFVN"
     gene            9527..10966
                     /locus_tag="PKW26_02565"
     CDS             9527..10966
                     /locus_tag="PKW26_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HOQ92483.1"
                     /translation="MKRRDAIHIRLPGERFTPFSLLKKLNAKAILESASFTQGRARYS
                     ILLLEEAFRLMQDNSGIYFLHGSEKKRLEQSDFLAPENRQAPYDILDLLSYIANQNEP
                     AQDGIPLPGSGIGYISYEFVQRTYGIDFSGQKDDLGMPESLFIVGHIYLVFDHFKEII
                     HIIGLNYNESEIDLEKKVFEVKERIENLDFTYLAQAESKSDYRILTNIDASKKEYCKN
                     VSKIKDLIREGEVVQAVPSRRLQFESELDALEAYRRLRSLNPSPYMFYIDFSSFQLIG
                     ASPESVVRVENGFATIRPIAGTKKRGKNRDEDEMQKELLENDPKEKAEHLMLVDLARG
                     DLSRVCVPGTVELSSFQKPEFYSHVIHLVSEVRGKLDKNINSTDVFRATFPAGTVSGS
                     PKKKVMELISKLESSKRNFYAGAVGYMQSSGDLDFCIAIRSALKQGKIWTMQAGGGIV
                     FASDEEREWEETNEKLSAFSHIFEMKDIK"
     gene            10978..12603
                     /gene="trpD"
                     /locus_tag="PKW26_02570"
     CDS             10978..12603
                     /gene="trpD"
                     /locus_tag="PKW26_02570"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOQ92484.1"
                     /translation="MVLIIDNNNESTHNIVHAIKKYSRHDVFLAQEESIDLEQVKALE
                     PEVLIFASLSFPKMRDEGKNILARLKEVVDYYAHKKLLLGIDSGFLFLAYCFGAKIKA
                     VEKIEQALVKEIKLDGRGLFRSIGNKANFVLYNCFYIEEESLSKDFEITAKTNEGQIM
                     GFRHKTMPIEAVLFHPESYASEYEKQFFVSVLNYRREALPVQEIVTRLCAGEDLDRLS
                     AEIFMEDLTDGCLDERQTAAILTALTAKGPSEEEIAGCAAVLYRKKAPLEIGTRELTD
                     IVGTGGDSKGSFNISSMAALLASSCDLAIAKHGNRAVSSKSGSADFYEALGIKIDTSA
                     EHTARAIKKTHFGFLFAPLYHAAMKHAAPVRRAIGIKTIMNLIGPLSNPAGAAYQMLG
                     VYDLSLLAPVARASKLLGAKRVMVVCSLDGFDEISPCEKTAVFEIDEDGNEKNYFLDP
                     KDFEIENCDAKELDGGTAEENALLAFDLMEGRGLKAIVEAVSLNAGAALYIGKKAGSI
                     EEGYRMAKEAIISGRLKEKVKEIQEETNAKESA"
     gene            12584..14188
                     /locus_tag="PKW26_02575"
     CDS             12584..14188
                     /locus_tag="PKW26_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92485.1"
                     /translation="MQKKAHKIEDSGDIKTENILDLIVAERKKTIEKKGYNFGFKIPE
                     KREKPIIPFMPDRGLILEIKRASPSKAWISKDLDIKAKASEYISLGAKAISVLTEEEF
                     FKGSLKDLVAVSNEVYKKNKRVAILRKDFLSSVEDIAISYYCGADVVLLIASVLFYEN
                     SYLMMKEALKFGLSVLYEVRTKEEFLIYESCLEHLEKEAYLDVREKIVLGINARNLKS
                     FKMDMLKPLRFLNLSKKKTKIIFESGIKTKEMATRLAHLGFFGLLIGEAAVKEKKEIK
                     KIAESFGKEKNTRDSVFWKHFLADGFLEKKIQKKICGFTHLDDVLMAIEEGVEILGFI
                     FSKKTKRKASKELLELLAKTDIYKKYSQDKKLLRVGVVTELYSKEWRQAISLLRHGYL
                     DVLQIHDLEVFHTAHKKNLVKVLEGLRKKNIAYYTAIALEDEADLRALSRLQDLGEFR
                     VLIDRKKVSNSKNTEANQKEGIVEYIKLLEKVESPLWLAGGLSAELVLLYNEQLDIEL
                     LDFARATEGAIVGRKEKEKIETVLAL"
     gene            14231..15508
                     /gene="trpB"
                     /locus_tag="PKW26_02580"
     CDS             14231..15508
                     /gene="trpB"
                     /locus_tag="PKW26_02580"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOQ92486.1"
                     /translation="MNKEKADGFYGMYGGSFVAEIIRRPIDELEKAFRKQSQDYTFLT
                     ELEELRRDFIGRPTPLLFAEKTSRENGGAKIYIKAEGFAHTGAHKINNAVGQVLLAKK
                     MGKTRIIAETGAGQHGLATAATCARLGMECTVYMGEVDVLRQQPNVCAMEMYGAKVVS
                     VKTGARTLKDAINEAMRDWASSFEHTHYVIGSALGPAPYPEMVKFFQTVIGKEVQKQS
                     MEKNLSIQALVACVGGGSNAIGFFAPFIAEKSPRLIAVEAGGKGPGLGENAVRMKGSA
                     RTSIVHGYKSKFLVDEDGQVRNTYSISAGLDYTGIGPELASLGEEGRIEFTSVGDREA
                     LDALQLFAQNEGLLFALESAHAAAAALTIAKTMKANEVVVVNMSGRGDKDLFISAPVF
                     RADSWKLFLQNELARLETGTDVHDMKMQMGEEA"
     gene            15505..16269
                     /gene="trpA"
                     /locus_tag="PKW26_02585"
     CDS             15505..16269
                     /gene="trpA"
                     /locus_tag="PKW26_02585"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOQ92487.1"
                     /translation="MKQKIKLMAHQIALYPNKEAALLYAQALVEGGAEILEIQIPFSD
                     PSADGPLIQEACAQTIAAGVRVKDCFAFIKSIHEAFPKLCIYLMSYASILVSYGLESF
                     VLESKAVGVSGFIVPDLPFDSNEGLIDFAEAQGLLYIPVITTSMSEERLERILACGFT
                     LVYCAIRKGITGSKTEIADETIVFLDGLKNRGIKVLAGFGIVEKSQAELLAPHVEAIV
                     AGSVFVEIMQKQIKENLAQDKIVLLLEEKSKELSGK"
     gene            complement(16296..17330)
                     /locus_tag="PKW26_02590"
     CDS             complement(16296..17330)
                     /locus_tag="PKW26_02590"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOQ92488.1"
                     /translation="MNNTHSKTQIDTHSNTHRTSEKEKDKNQNSQSANNPVDLSVNIA
                     GVHFKNPVIAASGTFGFGEEYAQLVNVDALGGICSKGLTLEARQGNTGKRLHETASGL
                     INSIGLENPGIETFIKRQLKTMLSFDTVSIANLSGSTIESYVEGARLLDETEIPMIEL
                     NISCPNVKQGGMAFGLDPISAAEVTTAVRKATRKPLIVKLSPNAPKLLSVAEAVQESG
                     ADALSLINTIQAMAINIERGKPVFDNVKAGLAGPAIKPIALSMVWDVCKMMQEKKEKD
                     RIPVIGLGGIRNWIDAVEFIMAGASAIQVGVATFANPQAMEEIIAGIETFMKRKNYTT
                     IEDFRGLALE"
     gene            complement(17341..18273)
                     /locus_tag="PKW26_02595"
     CDS             complement(17341..18273)
                     /locus_tag="PKW26_02595"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92489.1"
                     /translation="MRRRVLQSQTITKVCEIKQLQKDVFSLKVETELKHKEQSIPKAG
                     QFYMLKSRPSRVLLTRPISVYKVRLLDASGKELASHADCLQETFPAVQDAHVLELEFL
                     ILQKGEGTEDLCNLQVGLSIDLIGPLGNHFFKPSESLSGAKNSKAFAEREAAKEANTE
                     NQLPPSVAIVGGGIGIAPVSGFAQGLEKESYDFYASFKSGTYGIEDVQAKNLYITTDD
                     GSVGVHGMLPAVLTADTLKEKKYTLLYACGPLPMLAYLQKICLEAGVQAWLSMEEKMG
                     CGVGACLGCTIKTTEGNRQCCKAGPIFQGDKLIF"
     gene            complement(18285..19310)
                     /gene="pyrF"
                     /locus_tag="PKW26_02600"
     CDS             complement(18285..19310)
                     /gene="pyrF"
                     /locus_tag="PKW26_02600"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOQ92490.1"
                     /translation="MNYLHEMHRLQASCKEKGPLCIGLDTDPSYLPESILSDYAHPAE
                     AVLAYNKALIDATKNDTGCYKVQIAYYEAMGLKGLSCYLETLRYLRSLDIPFIADIKR
                     GDIADTAKAYARAHFSGDFEANTITVNPYMGFDTLEPFLEWCKPKTNNTNPDAREKTA
                     STDKSAPLPKGFFVLLRTSNPGMVDIEKQALASGGFVLDRVGEKLLSIEKEMTSLYAN
                     AGLSKTASNNTNVCSPVGAVVGCTEESDARALRDAYPNIFFLIPGYGAQGGAARIAAT
                     LLDKAGGTVNSSRGILTAWKNDSLLEEKRSAEKLCLKDIVESAQRAAKNAKEDLLNAK
                     KSLQINN"
     gene            complement(19389..21446)
                     /locus_tag="PKW26_02605"
     CDS             complement(19389..21446)
                     /locus_tag="PKW26_02605"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOQ92491.1"
                     /translation="MKRKKKAPVFLRSLFISILVFSLSILSFFIPLTTRLDFLLYDYA
                     MRLTASFTHPSDAISLVLLDQESIDWALQEKGWNWPWPREAYGDIVRYFNLANAASLT
                     FDVLFTEPSLYGNADDENFAQACRENGKVVHTFYYSDKNKPPLLPIPSIRQSAALLGD
                     VTGTSDEDKVARRVKSFTTYNGEKIPSLGLAPLLAAGIAVETDKPENPKGQLLRFQEN
                     LDRYIPYSASDILQSYYAIQKGDAPLLEAEQFAESFIFFGFFAPGLFDITATPISTAY
                     PGVGVHVTQLDNYLQNSFIKEIPFILLALALLLSSFVSILPIGIHEKLASNARYAKLS
                     GILTTISFIFLIASYSLVYFFLFYKGVYVPLASFFVSLFLGFAISVIISYTQEGKQRR
                     YIKNAFKQYLSPIVIEQLIANPDKLKLGGERRNLSIFFSDVQGFTSISEKLNPSDLTS
                     LLNDYLSEMTDIILASGGTIDKYEGDAIIAFWNAPLDLENHAQAALEAAVKCQSKLDA
                     MRPELEKRAGQVFLMRIGINTGDAVVGNMGSHSRFDYSMLGDSVNLAARLEGFNKQFD
                     SYTMCSEATKNAAQRHGCRLRFRELGKAAVVGKSQAVRVFEPMTEVAYENKKQSIDIF
                     EKALKAFYDGDFLAAKELFTSIAATDPPAKKYLEKLIFYENNPLSEEEKKNWEGIWVA
                     TTK"
     gene            complement(21462..22343)
                     /locus_tag="PKW26_02610"
     CDS             complement(21462..22343)
                     /locus_tag="PKW26_02610"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOQ92492.1"
                     /translation="MKKAKSLVILLGSLTLLSFFFSCESISDFDTLGRVVSSGVDAGM
                     AIAKAAEPITPEQEYYIGRSVAATLLQSYDLADEVKLTAYLNKICMALVIHSERPELY
                     NGYHVSILDSDQINAFATSGGHILITRGLLSCAENEDALASVIAHEIGHIQLQHSLKA
                     IKTSRSSQAALATGGLFLNLTGNEDLMDLADMMDASINEVITTMVNKGYSKQQEYDAD
                     AAAVKLLFDAGYNPHAIISMLKVLQEKQPRSSGGFAKTHPSAKQRINEVNKVLKKYAT
                     SPEAINRNKRFNETVFL"
     gene            complement(22340..22810)
                     /locus_tag="PKW26_02615"
     CDS             complement(22340..22810)
                     /locus_tag="PKW26_02615"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOQ92493.1"
                     /translation="MKKHVSIVLIFLFLLPCVFAKAGDTMYVNVKESILKSGTGFFAS
                     KLGTVYYGDTVIILAEAGKWTQIELKENPSNKGWIPSTNLTKKKIISGKTQNLSESEL
                     ALAGKGFSAEVEDAMKQRHGELRFDLVDKIEDNYVGDEDLLEFIVEGKLYGGEG"
     gene            22974..24338
                     /locus_tag="PKW26_02620"
     CDS             22974..24338
                     /locus_tag="PKW26_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOQ92494.1"
                     /translation="MSESIVQKTNPLGTEKVGTLLRTFSIPSIISMLVGALYNIVDQI
                     FIGQKIGELGNAATNVAFPLSTASVAIALLMGIGGAAAFNLASGRGETEKGGYIIGNS
                     VSILIILGIVLMLITQAFLEPLLRFFGSPENVLGYAKTYTGIVSFGFPFLILSTGATH
                     LIRADGSPKYAMFCNLSGAILNTILDPIFIFVLDMDMAGAAIATILGQMLSFVLAIGY
                     LRKFRTVKLVFSHFIPRVKYFSQSMILGLAPAFNQSAMMLVQIIMNKSLVFYGALSAY
                     GAAIPLASAGVITKTAMIFFSIIIGISQGMQPVVSFNYGAKKYDRVKEAYKTSLKVAL
                     FISILAFLLFQLFPRQIVSIFGKGSAEYFAFSTLYFRIFMFFTFINCIQPVSANFFTA
                     IGKPHKGMFLSLTRQIIFLLPLIFLLPLRFGIDGVVYAGPIADFFAASVSVFFISYEL
                     KKML"
     gene            24415..>25436
                     /locus_tag="PKW26_02625"
     CDS             24415..>25436
                     /locus_tag="PKW26_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOQ92495.1"
                     /translation="MIKKIPLLCFCVLFFSFIFGETQEVLKLSLDDAVLLAMQNNVQL
                     SSMAIDLRMKKAEKDYAWNVFLPSIQATGTMARTNIKEASATIPSMSPPGFATITTKL
                     DEKDKWKVMGGLNISLNLNAALFEGLRATRQSYEAGLLSYEAARRDTEQNVKKAFYGL
                     LLQEGSLAISKEKLASAQKRKTQMEANFKNGLVSELQYLQVQLALETQKPAIMEAELL
                     LAQQKELFAFLLGLPLGTEIVLVGEIAPHIQEFNADELLAEYLGRSFELAQLDKNKEL
                     METQLRATLLQTYTPTIVLSQTFNPALGKIDDDWFKKDNWADTSGAFSLTIAFNLTNL
                     LPFSGMG"
BASE COUNT         7064 a         4782 c         5654 g         7936 t
ORIGIN      
        1 agaaagcttt ttctctcctt gtatgctaac aaacgtattg catttactat aacaagaata
       61 accgatgctt cgtggacgag cataccgaga gaaagattta cggtttttat aagcactcca
      121 gcaaggagaa aggctgctac agcaagcgca aagctaatgt tttgtctcat attttttacc
      181 gtagcacggc ttaaacccat tgcatagcga agtttttgta aatcacttgt catgaggaca
      241 atatctgcag tttccatggc aacatcgctt ccctcttcac ccatggcaat gccaacaaag
      301 cttgctgcaa gcgcaggtgc gtcgtttact ccgtcgccaa ccattgcaac tcgcggatag
      361 tcttttttta ttttttcgag cgcatcgacc ttatccgctg gaagcaattc tgcatggaca
      421 gcactaagcc caaggttttt tgcaatagct tgtgcggttt tttcattgtc cccagtaagc
      481 ataataatct tttttaatcc cagttttttt aagtcgcgta taatttgttt tgcttctgga
      541 cgtatttcat ccgcaatgga aagaaagccg agtatctcat tttctgttcc gaggataata
      601 caggttcttc cctctgattc ttcgttagag agaagctctt ctaggtttga gccatcttgt
      661 gttttaagac ttgtaaacaa gcttctattt cctatgcgat attttgtatt cttgtgacca
      721 aaaacgatgc cttgcccgat tatcatttcg gggttttcag gcgagtcgag ttgaagcgcg
      781 gcatcggttt ttttgagctt tgcatctgcc atgcgaagca cagcctttgc gaggggatgt
      841 tcggaaaaag attctccaat ggcggcaatt tctaagattt ctgtttcgct taaagcgctt
      901 gttgtctgta ttttttgtac ttcagctctt ccctttgtga gcgtgcctgt tttatcgaag
      961 gctatggctt taactgtccc aagtttttcc attacctctc cacctttaac gaggacgccg
     1021 tgttttgcgc catttccaat tcctgcaaca atggatacag gagcggatat aacaagggca
     1081 ccggggcagg atataacaag gagagtaagt gcgaggcgaa aatctcttgt gattagatag
     1141 acggcaatac tcaagaggat gatacccggg gtgtagtagc gcgaaaatcg ctctaagaac
     1201 ttctgtgttt ttgctttttt atcttgtgct tcttctacaa gtcgaaggat tcctgcaaag
     1261 gtcgtgtctt cgccaacctt cactgcctcg attataatat agccactttc gacgatggtt
     1321 ccagaataga ccgatgagcc aagctctttt tcgagaggca tcgactcccc ggttatagct
     1381 gcctgattta ccgaagcaaa tccttctata accttaccgt cagcagggat tttttctcct
     1441 ggctttatga taatcacatc gcctatgact acttcttctg caggtatttc gagttctgcg
     1501 ccattgcgtt ttacccgtgc ggttactggt gcgaggtcga gcaggctttt tatagaagag
     1561 cgtgtttttt ctatggtgcg cgcttcaagc cattctccga gcatgaacaa aaatgaaacc
     1621 gcagcagctt cccaatactc tcctataaaa atagcaccga tgatcgcaat tgttacgagt
     1681 aaatctatcg caacaatttt atagcgaaga gaagaaatgg ctcgtgccat gatgggcaaa
     1741 ccagagatgc ctgttgccaa aatcataagt atgattgtta tgagatggta gccgttttcg
     1801 tgattatcct tgagtgcaat tttaaaaccg agggcgacga ggcttacaat tccagaaagt
     1861 ataagccgcc ctttctttga gccacttcta cctagaacaa gcgaaataaa agatgccata
     1921 ttgagtctcc taaaaataat gggaaggttt aatttttaaa agcaaaactc ccctcgctat
     1981 aaaataagcg agaagagaaa gcgttttact tttcgtttaa tacaacaaaa ccgaggtcgg
     2041 cgatgttttt ctttatttga tctgagttta cttctgcttc atcaaaggaa gcccgcactc
     2101 gagaggtgtt aaataaaact tcactttcac tgatgccctt tgtttttttg agcatgcctt
     2161 caattttggc aacgcaagaa gggcaggaaa gagtttccaa ttgatatgtt tttgttgtca
     2221 taaaaaacct ccatagtctt ttctttatag taattgatgg aaacatttta ctatttccta
     2281 ctgttttaat atactataaa agagaaaaaa agtatagtgg aaaggggata aaagaggctt
     2341 tggagcttta aatagtatga ttttagtata ggactcttag atattcttgt atctattata
     2401 acagcaggta taaagataat acaagcaaaa ttttttgaca aaaaaagggc aacgatttga
     2461 aataaaagtt gcccagtttt aatatttgct ttctttagcc aaagagtttt ttgacgagtc
     2521 tttcttcaag tagttttgcg ccatctccgc gtggtgcaag ttcgtaagca acatttatta
     2581 ttggtttgtt gactttgacg actcgcccta aatcgatagg agtaccgctc aatacaaggt
     2641 cagcctcgat tttattgatt gttgtctcca aatctttttt ttgtgcatcg ctatagccca
     2701 tcgcaggtaa aatacccttg cctatgttgg gatatttttc ataggttgaa gcaatttttc
     2761 caacagcatg tggttttggg tcaacaattt ctgcaacgcc aaaacgctca gcggcgacac
     2821 ttgctgcccc tattttcatt tccccatggg tgagggtagg accatcttcc acgattaaaa
     2881 ctttcttgcc ttttatgtcg ccatctaccg tgagtgcgct gtctgcttct atgatgagcg
     2941 cctttggatt aagccgttga caattggcct ttaccgttgc tatatcctct ggctttgcgc
     3001 tatccatttt atttataacg ataatatctg ccatgcaaag atttacttca cctggataat
     3061 acgaaacctc gtgccccggt cgtagcggat cgacgatgac gatttttaag tcagagtcat
     3121 aaaagggaaa gtcgttattc ccgccatccc acaaaataac atcaggctca ccgtctgcat
     3181 ctttttctgc cgcgcgaata atggcctcat aatcaacacc ggaataaatt acatttcccg
     3241 cgatgatatg tggttcgtat tcttccattt cttctatagt acatttgtgt ttttctaagt
     3301 cagaaagctc tgcaaaacgt tgcacttttt gagcaacaag atcgccatag ggcatgggat
     3361 gtcgaatcgc aacaactttt tttcccattt ttttaaatat gtcatagata aagcgagttg
     3421 tctggctttt tccacaaccg gtacgaaccg cacaaatgga aataactggc tttgtcgatt
     3481 tgatttttgt tcggtcatag cccattaaaa taaagtcggc acccaaggca tttaccattg
     3541 cgctccggtt cataatgtat tgataagtta catcactata ggcgaagaca acttcttgaa
     3601 ccgccaattc tgtaataagc cgtgcaagct cagactcatt gtagatagga attccgttcg
     3661 gatataaatc ggctcctgca agctctgcag gatattttct cccgtcaatg tcggggattt
     3721 gtgttgcggt aaatgcaaca acctcatact ctggattatt tctgtagtag gtgttgaaat
     3781 tgtggaaatc tcggcctgct gcacccatta taagtacttt ttttctagac atacatgcct
     3841 ccttctatat aatgcaataa agttgccttc tttgtcaatt attttgtttt gtttttttta
     3901 actcgatagt gtaaaaattg aataagggta gatagaaaaa gcttaaaatt gttttgttga
     3961 aagcatcacc aaagtgcagg cttcaaggta gactatgcca gccgccgctg ctttttctgg
     4021 caaaaaagta ttttctgtac caggattgga aataatacgc cttggtttta aagcaagaat
     4081 ttcatcgaca taggcttcta ggttttgtgg accgagatag agcgtaatag tgtcgatagg
     4141 ctgatcgatt tttgagagac tttgtataac aggccgcccc tcaatctcgt ccagctttgg
     4201 attaaaaaga aaaaccctat gaccagcttt ttccaaccgc tccatcgcca tgtacgcata
     4261 acgttctttt ttattacttg caccgataat taaaacattc atacagataa agtactatga
     4321 aatgagaaga aaaactagaa gggagcacgg ataagcacga acagcttctc gagagctatc
     4381 acaaaaagcc atatttccag tgccagagtg gtttggcgag ttcgtttggg aaggtttgtg
     4441 tttggttaaa gtctgggagc cagtcatcgc tggggtttag ttcttggtaa aagccgtttt
     4501 ctatttcaaa gtaatcgagg atgtcaaagg cggtttcaag ttcgtattct tctaaaaatc
     4561 tattttccat gagttttatt gcatcgattt ttttctccct tcctcgaatg ggtgtgtatt
     4621 ggctcatgag tgagaggtag cccttggtgt cgatgttttc tttgaaccat tggataacgg
     4681 aaaagctgtc ttcaagtcga ttggggagga tgaggtggcg gacgatgagg ccttggagca
     4741 tcttgggaag tgtgaggctt tcaaattcca gtatgctgtc ttttttttgt atcgttgagt
     4801 tttgaatttt tatcttgctt ttttccatca tccaatatat tgcttttttt gcatattctg
     4861 gataattttt tgcgcgaaag gcttggctgc taaaggcgtc atcgagggtt tttaagtcgg
     4921 gtagccaaat atcgacgagg tttttgagga gttcgagggc ttcgacgttt tcgtaggcgg
     4981 agctattcca gcatacggga atgcttagtc cctttttttt tgcttctttt aagccaaggg
     5041 cgagggcagg aattgcgtgg cttccggtaa cgatgtttat attttctgcc ccttcttttt
     5101 ctagtttgag gcagattgtt gcaaagtctt cggtgcttac gacggagccc attttttctt
     5161 gcgagatttg aaagttttgg caaaaggcac agcctaaatt gcagccgctt acaaagattg
     5221 tgcctgagcc gccgcttcct gttatgaggg gttcttctcc aaagtggaga ccagcaaagg
     5281 caaggcgaag ttctgcgctt tcgccacaga tgccgagttc gcccttgttg cggtttacct
     5341 tgcagtcatg gggacagagc cggcaagagt cataggctag taaaagagta ttcattttcc
     5401 taccatacac tttttatgct ttttcgtata ttggttacta ctttttttag cctatatcga
     5461 tttctggttt aatgcactga agataaaatg cttgccttgc tcactaagcc tttgagtttt
     5521 ttctcttcgt ccaagactgc gataggataa cgggtttcac ttgctatggg aatgatttcg
     5581 ctaattgcgg tgtcttcgct tgtcgtttct atatctgaaa taataatttc ttttagcgct
     5641 ttgttttctt gcatcgcttg tatgacatcg ttaatcaata caatgcctaa aaaatgcatg
     5701 tcgttatcaa cgcaataaac tgatgagacg tcgtttgagc gcatttgtcg taaggctgtt
     5761 ttaagagagt ctctttcttt tactagggag cgtggtttta tcattacatt ttttacggtg
     5821 agcacctggc ttttatcagc gccttctata aaatcttcca cgtagctgtc ggcaggatgg
     5881 gtggacattt cttcggggct gtctatctgg acaatttttc cgtctttcat tatgccgacg
     5941 cggtcgccga gtttaaaggc ttcgttaata tcgtgggtaa taaaaacaac ggtctttttt
     6001 aatttttttt gtatcgataa aagttcaaac tgcatttctt tacgtacgag tgggtcgagg
     6061 gcagaaaagg gttcgtccat caataaaatt tctgggtcgg ttgcaagggc gcgtgctata
     6121 cctacgcgtt gactcatgcc gcctgaaagg cttgtgatgg ggtggtgttc aaagcctgag
     6181 agcccaacca tttcgattat ttcctgtgct ttttcttcac gttcttcttt tcctacgcct
     6241 tttatttcaa gaccgtagac tacgtttgaa agtacatctc tgtgagacat aaggccaaaa
     6301 ctttgaaaga ccatagcgat atgatttcgt ctatagtctt gtagttcttt ttttgtaaag
     6361 ctttgtattt tttttccttt gaaaaagatt tcacctgaac ttggtttatt gagtcggttt
     6421 aggcagcgta ccatggtaga ttttcctgag ccggagagtc cgatgagtac aaagatttcg
     6481 ccttctttta catcgaagga tgcatcccaa agggcaactg agacgcctgt tttttttagg
     6541 acttcttcct ttgttgcacc tttttgcaaa agttttattg catcgccttt gtttaagccg
     6601 tagagttttg aaactttttt tgcttcgagt attattttag tatcattgtt ttccatttgt
     6661 ctgtcctttt tcggcaatgc cctgtgttat gcggtcgagt acaattgcga gaatgactac
     6721 tgcgattcct gctatgagtc cacgtcctat ttctattctt tgtactccca taagtacttc
     6781 catgcctaag cctgaagcac caatcatgga gcaggttaca accattgcca ttgccatcat
     6841 gagggtttga tttacgcctg tcataatggt agcttttgct tgtggaattt gcaccttgat
     6901 aagtgtttgc actcctgttg agccaaaggc ttttgccgcc tctactactt ccttatcaac
     6961 ttgtcgaatt cctaaactcg taagacgaat taccggtacg atggcgtaaa ttgttgttgc
     7021 gataactgcg ggagcttttc ctaagccaaa aaataaaagt gcggggatga ggtagacaaa
     7081 gacgggcatt gtttgcattg tatctaaaac agggcgtgca attttttcag ctctgtcgaa
     7141 ttgtgccaag agaatgccga gtggtaagcc aaaaaaaagc gatataataa ctgcggcgat
     7201 tatgattgaa agtgtttcga gcataagtgc ccaaagtccg gcgatgccga taactgttat
     7261 agcgagggca aaaaaaatgc cgtttttaag ttttccggtg ctcttccatc ccgcaaaaaa
     7321 aattgcgagg ataaaaagcc accaggggat aatgtttaag aggctgtgga ttccgcctat
     7381 gagaccattt aagcccgagg ttatggctga aaaaaaagca tcgtgtttta cgctaaatga
     7441 acgaactgcg ctgtctatgg cttttttatt gattgtaatt tcaaatggaa aatctattaa
     7501 ccatttcata ttttctcctt attgttagaa aaaagaaaac gcttgctaca aacgtaggta
     7561 tcaagcgttt tcaaatgagt agggttagag agcttgtcga acaagcgctg ccttatcttc
     7621 tgggagccag aggtcgagta aattatcgta ggttttcata agccagagcg ctgtttctgc
     7681 tgcagaggca ttattgttgc tcatgtaggc aagtgcttca gaaataaggg cgctgctggt
     7741 tttgtagttt gcaacaaatt cgttacaaaa ggcaggatct ttttcataaa aggcattgct
     7801 taccgctgtg gtacaaacga cagcagggca ttctgttttt cctgccatat agctttcctc
     7861 atcgatatag ggggcgtctt cgagtaagac gaggtcgtag agccccatga gccaggttgg
     7921 ttcccagtaa tatcctacga tcgcttgacc cttgtcgatg gcagaggtga aggcggcagc
     7981 aagagccgca tcagagcctg gtctaaagta gataaagttt tcgtctaagc cataatattc
     8041 gtatttgttg aacatgatgg tatcgatttc ccagccaggg atagagccat atattctacc
     8101 cttcgttttg ttttctgggt cttgaaaaac atctggatag agcgcaaggt cttgtacggt
     8161 ttttaagttt ggtgcagatg cttcaattcc tcgactcgca tcaccttcga taacatagcg
     8221 tggcacataa aagccttgca tattgtcatc aaagtttacg cccatttctt tcagttttcc
     8281 tgcatcacgg tctgctgtgt atgaggcaat attatctgtc cactcttcta agtgcacatc
     8341 gatttcaccc ttaattgtac cctcgtgagt aatcggtgtt gttccggtga tttgttgcca
     8401 tgagtcgtag ccgtaggcgt ggatggcaat gaaacctgcc acctcgttgt gtaattgcac
     8461 ggaatcccag ccgggatccg caaagatgat tgtctttttt tcttcggtag tacaaccgat
     8521 aaaaaaactc agtgttaaaa taagcccaat aaaaataaga gagccctttg taatgctttt
     8581 cattttcttc tcctgttttg aaaatctgaa catattgttc aagcacatag catggatagt
     8641 aatgagtcaa tacaaaagtc tgttgtttgc atcaacgtta attcacaaag agatttttta
     8701 cgcgttcata ggattccttt ggtccgataa aatacaatag atcgccttct aaaaactcag
     8761 catagggtcc gggagataaa agcatttcat cccctcgtag aatagcaatt atcgttgcac
     8821 cagttacttg ccaaaattgc aagtcagaac ttgtttgtcc tatatgattc attgaagcaa
     8881 gcaatttaaa ggtgaaaggg gtaaagggac ttatcgcctc ttgtcgttct gtcatgtcga
     8941 gcaattttcg catggtcgaa cgcatcgcat cagtctcttg cttttgtcgt tgcatccatt
     9001 caagcaagtc gtttcgaagt tccttaatcg aatgcgcact tgtgtattgc tctagaaaag
     9061 cctttgcctt tagtgccgat tgtatacaaa tgccgctact cggttttatc gatacaacct
     9121 ggacatcctg caagactgcc attgccttac gaatcgtttc agatgaaacc ttgtatcgac
     9181 ttgcaagcgt tgagcgcccg gagagttttt ctcccaccgc atatttgcca tcgacaatat
     9241 cttgcgcaat actcaatgca attttttgat acgaagccat ttttatcgtc gccatgaaat
     9301 ccccaaagta aaaaaatagt ctactaagta gtttctcttt gtccgacaac ccaagtataa
     9361 caataggctg tcggtttggt caagtagata cattgctcag attacattta agctaagatt
     9421 ttgtatacgt tttttctcaa aaataacttg acaggctttt tgtttctctg tatagtatca
     9481 ttgagccatg ttactgtgtg tagaaacata gcgcaatgga ggaaaaatga aacgaagaga
     9541 tgctatacat atacgtttgc caggagagcg atttacgccc ttttctttgt taaaaaaatt
     9601 gaatgcaaag gctattttag agtcggcttc ttttacgcag ggaagggctc gctattctat
     9661 tctcttgctc gaagaagctt ttcgacttat gcaagataac tcgggcattt attttttaca
     9721 tggtagtgaa aaaaaacggc ttgaacaaag tgatttttta gcaccagaga atagacaggc
     9781 accctatgat attttagatt tgctctctta catagcgaat caaaatgagc ctgctcaaga
     9841 tggcattcct cttccgggta gtggaatagg ctatatatcc tatgagtttg ttcaaagaac
     9901 atatggcata gatttttccg ggcaaaagga tgacctcggt atgccagagt ctttgttcat
     9961 tgttgggcat atctatcttg tttttgatca ctttaaagaa attattcata ttatcggttt
    10021 aaattacaat gaaagcgaaa ttgatttaga aaaaaaagtc tttgaagtta aagaacgcat
    10081 tgaaaatctt gattttactt atcttgcgca agccgagtca aaatctgact atcgcatact
    10141 gacaaatatt gatgcatcta aaaaagaata ttgtaaaaat gtttcaaaaa taaaagacct
    10201 tattcgagaa ggggaagttg tgcaagcggt gccgtcaagg aggctgcagt ttgaaagtga
    10261 gcttgatgca cttgaagcgt atcgtaggct gcgctctctt aatccttcgc cctacatgtt
    10321 ttatattgac ttttcttcgt ttcagttgat aggagcttct cccgaatctg tcgtgcgggt
    10381 ggagaatgga tttgcgacca ttcgtccgat agcgggtaca aagaagcgag gaaaaaatag
    10441 agacgaagat gaaatgcaaa aagagctgtt ggaaaatgat ccaaaagaaa aggctgagca
    10501 tcttatgctt gtcgatcttg cgcgtggtga tttatcccgt gtttgtgttc ccggcacagt
    10561 tgagcttagt tcatttcaaa aaccagagtt ttacagtcat gttatccatc ttgtctccga
    10621 agtaagggga aagcttgata aaaatataaa tagcacagat gtgtttcgag ctacttttcc
    10681 tgcggggaca gtcagcggtt ctccaaagaa aaaagtaatg gagcttattt caaaacttga
    10741 atcaagtaaa agaaattttt atgcaggagc tgtgggttat atgcagtcat cgggggattt
    10801 agatttttgc attgcaattc gttcggcgct taagcaggga aaaatatgga ctatgcaggc
    10861 aggcggtggt attgtttttg cttctgatga agagcgtgaa tgggaagaaa caaatgaaaa
    10921 gctatctgct ttttctcata tttttgagat gaaagatata aaataaggaa gtgtaaaatg
    10981 gttttaataa ttgataataa taatgaatct acacataata ttgttcatgc tataaaaaag
    11041 tattcacgtc atgatgtctt tcttgcccaa gaagagagta tcgatcttga gcaagtaaaa
    11101 gcgctggagc cagaagtttt gatttttgca tcgctttcgt ttccaaagat gcgtgatgaa
    11161 ggaaaaaata tcttagctcg attaaaagag gttgtagatt attatgcaca taaaaaattg
    11221 cttcttggta tagatagtgg ttttttattt cttgcctatt gttttggtgc aaagattaag
    11281 gcggtagaaa aaatagaaca agctttggta aaagaaatta aacttgatgg ccgtggtttg
    11341 tttcgttcta ttggaaataa ggcaaacttt gttttataca attgttttta tatagaagaa
    11401 gaatctcttt caaaagattt tgaaataacg gccaaaacaa atgagggaca gataatgggc
    11461 tttcgtcaca agacgatgcc tatagaagct gttttatttc atccagaatc ctatgcttca
    11521 gaatatgaaa aacaattttt tgtatctgtt ttaaattata gaagagaggc attacctgtg
    11581 caagaaatag taacaaggct ttgtgcagga gaagatttag atagactcag tgcagagatt
    11641 tttatggaag atcttacaga cggttgtctt gacgaaagac aaacggcagc catacttacc
    11701 gccttaaccg caaaggggcc ttcagaagaa gagatagctg gttgtgcggc tgtcttgtat
    11761 agaaaaaaag caccgcttga aattggtacg agagagctca cagatattgt tggcacaggt
    11821 ggagatagca agggaagctt taatatatct tctatggctg ccctactcgc atcgagttgc
    11881 gacttggcaa ttgcaaagca tggtaacaga gcggtgtcca gcaaaagtgg ctcagctgat
    11941 ttttatgaag ccttgggtat taaaatagat acatcagctg agcataccgc acgagcaatt
    12001 aaaaaaacac attttgggtt tttatttgct cccttgtatc acgcagcgat gaaacatgct
    12061 gctcctgttc gccgtgctat cggtattaaa acaattatga atctaattgg ccccttgtca
    12121 aatccggcag gagcggccta ccaaatgctt ggcgtctatg acctgtcatt gcttgctcca
    12181 gtagctcgtg cttcaaaact tttgggtgca aaacgcgtaa tggtcgtttg tagcttggat
    12241 ggttttgatg aaatatcgcc atgtgaaaaa acagctgtct ttgaaattga tgaagacggg
    12301 aatgaaaaaa actattttct ggatccaaaa gattttgaaa ttgaaaactg cgatgcaaaa
    12361 gaacttgacg gaggaacagc tgaagaaaat gctttgcttg catttgacct tatggaagga
    12421 aggggcttaa aggcaattgt agaagcagtt tctctcaatg cgggagctgc cttgtacatt
    12481 ggtaaaaaag cagggagcat agaagagggc tataggatgg caaaggaagc tattatttcc
    12541 ggtaggctaa aagaaaaggt aaaagaaata caggaagaaa caaatgcaaa agaaagcgca
    12601 taaaatagaa gatagtggtg atataaaaac tgagaatatc ctcgatctca ttgtagcaga
    12661 gcgaaaaaaa acaatagaaa aaaaaggcta taactttggc tttaagattc cagaaaaaag
    12721 agaaaaaccg attattcctt ttatgccgga tagggggctc atactcgaaa tcaaaagagc
    12781 ttcgccgtca aaagcgtgga tttcaaaaga tttagatata aaggcaaagg ccagtgaata
    12841 tatctctctt ggtgccaagg caatatcggt gcttacagag gaggaattct ttaaaggaag
    12901 tttaaaagac ttagttgcag tttcaaatga ggtgtataaa aaaaacaaaa gggtagctat
    12961 cttacgaaaa gattttttaa gctcagttga agacattgct atttcttatt attgcggggc
    13021 agatgttgtt ttattgattg cctctgtttt attttatgag aattcctatc tgatgatgaa
    13081 agaagccctt aagtttggct tgtcagtttt atatgaagtg agaacaaaag aagagttttt
    13141 aatttatgaa agctgtttag agcatcttga aaaagaagct tatctggacg tacgagaaaa
    13201 aattgttttg ggaattaatg cgcggaactt aaaaagcttt aaaatggata tgcttaaacc
    13261 cctaaggttt ttaaatctaa gcaaaaaaaa gacaaagatt atttttgagt ctggaattaa
    13321 aacaaaagag atggcaactc gtttagcgca cttaggattt tttggattac tcataggaga
    13381 agctgcagta aaggaaaaaa aagaaataaa aaaaatagca gagagttttg gaaaagaaaa
    13441 aaatacgagg gattctgttt tttggaaaca ttttcttgca gatggttttt tagaaaagaa
    13501 aatacaaaaa aaaatatgtg gttttacgca tcttgacgat gtgcttatgg cgattgaaga
    13561 gggcgtagaa atacttggct ttattttttc aaagaaaaca aaacgtaaag cctcaaagga
    13621 actgttagag cttttagcaa agactgatat ctataaaaaa tattctcaag ataaaaaact
    13681 tttgcgtgtt ggtgttgtaa cagagcttta ttcaaaagaa tggaggcagg ccatatctct
    13741 tttgcgtcat ggctatcttg atgttttaca aatacatgac cttgaagttt ttcatacagc
    13801 acataaaaaa aacttggtaa aggtattaga aggtttacga aaaaaaaata ttgcctacta
    13861 cactgcgatc gcgcttgaag atgaggctga cctccgtgct ttaagtcgat tgcaagacct
    13921 cggtgaattt cgtgtgctta tagataggaa aaaagtaagc aattcaaaaa acacagaagc
    13981 aaatcagaaa gagggaatcg tagagtacat aaagctttta gaaaaagttg aaagcccgct
    14041 ttggctcgct ggtggtttaa gtgcagaatt agttttgctt tataatgagc agcttgacat
    14101 tgagttgcta gactttgctc gcgctacaga gggggcgata gttgggcgaa aagaaaagga
    14161 aaagatagag accgttttag ctctataggg caagacattt ttgtatttct caatttttaa
    14221 agaggtagat atgaataaag aaaaggcaga tggattttac ggaatgtatg gagggagttt
    14281 tgttgcagaa ataattcgcc gtccgattga tgaactagag aaggctttta gaaagcaaag
    14341 tcaggattat acgtttttaa ctgagctcga agagctacga agagatttta ttgggcgacc
    14401 aactcccttg ttatttgcag aaaaaacctc acgggaaaat ggaggggcaa aaatctatat
    14461 aaaggcagaa ggctttgcac atactggtgc tcataaaata aataatgcgg tggggcaagt
    14521 gcttcttgca aaaaaaatgg gaaagacacg gattattgca gaaacggggg cggggcagca
    14581 tgggcttgca acggctgcga cttgtgcacg tttgggaatg gaatgcacag tatatatggg
    14641 cgaggttgat gtcttgaggc aacagccaaa tgtctgcgct atggaaatgt atggggcaaa
    14701 ggttgtttct gtaaagactg gggctcgcac tttaaaagat gctataaatg aagcgatgcg
    14761 cgactgggca agtagttttg agcatacgca ctatgtcatt ggttcagccc ttggtcctgc
    14821 gccctatcca gaaatggtaa agttttttca aactgtcata gggaaggagg tgcaaaaaca
    14881 aagcatggag aaaaatcttt ctatacaagc cctcgttgcc tgtgttggag ggggctcaaa
    14941 tgcaattggt ttttttgcgc cctttattgc agagaaaagc cctcgtttaa ttgctgtcga
    15001 agccggcggt aagggaccgg gtttgggtga aaatgctgtt cgcatgaagg gtagtgctcg
    15061 aacgagtatt gtgcatgggt ataagtcaaa gtttttagta gatgaagatg ggcaggttcg
    15121 aaatacttat tcaatttctg ctggtttaga ttatacgggg attggtccag aacttgcctc
    15181 gcttggagaa gaggggcgga tagaatttac gagtgtagga gatagggaag ccttagatgc
    15241 cttacaatta tttgcccaaa acgaggggct tttatttgcg cttgaatctg cccatgctgc
    15301 ggcggcggcc ttgacaattg caaagactat gaaggctaac gaagttgtcg tggtgaatat
    15361 gtcaggcaga ggtgataaag acttatttat tagcgctcct gtttttaggg cggattcatg
    15421 gaagttgttt ttacaaaatg aattggcacg attggaaaca gggacggatg ttcatgatat
    15481 gaaaatgcaa atgggagaag aagcatgaaa caaaaaataa aactaatggc gcatcagatt
    15541 gctctttatc caaataaaga agcagctctt ctctatgctc aggcattagt tgaaggtggg
    15601 gcggaaattt tagaaattca aattcctttt agcgatccaa gtgctgacgg tcctcttata
    15661 caagaggctt gtgcacaaac aatcgcagca ggggtgcgcg ttaaggattg ttttgctttt
    15721 attaaaagta tacatgaagc ctttccaaag ctttgtattt atcttatgag ttacgcatct
    15781 attcttgttt catacggcct ggagtccttt gttttagaat caaaggcggt gggagttagt
    15841 ggctttattg ttccagatct tccttttgat agcaacgagg gactcatcga ttttgcagaa
    15901 gcgcaaggtc ttttatatat tcctgtaata acaacaagta tgagtgaaga gcgcttagag
    15961 agaattttgg cctgtggttt taccttggtt tattgtgcaa ttcgtaaagg tattacgggg
    16021 agtaaaacag aaatagcgga tgagacaatt gtgtttttag atggcttaaa aaatcgcggg
    16081 ataaaagtgc ttgcaggttt tggcattgtt gaaaaaagtc aagcagaatt attggcccca
    16141 catgttgaag caatcgttgc gggttctgtc tttgttgaga taatgcaaaa acagataaaa
    16201 gaaaatttag cgcaagataa gatagtttta ttgctagaag aaaaaagcaa agagctttca
    16261 ggaaaataaa aaaaacagct ctcttctgtt tctttctatt ctagggcgag acctctaaag
    16321 tcttctattg tcgtatagtt ttttctcttc ataaaggttt caatgcccgc tatgatttct
    16381 tccatagctt gtgggttggc aaaggttgca acgccgactt gtatggcgct tgctcctgcc
    16441 atgataaatt ctactgcatc tatccagttg cgtatgccgc cgagtccaat gacggggatg
    16501 cgatcctttt cttttttttc ttgcatcatt ttgcatacat cccaaaccat gctcagtgca
    16561 atgggtttta ttgcagggcc ggcaagtcct gcttttacat tatcgaaaac aggtttaccc
    16621 cgctcaatat tgattgccat tgcttgaatg gtattgatga ggcttaaggc atctgcgccg
    16681 ctttcttgta ccgcttctgc aacagagagg agcttgggag cgttgggaga aagttttacg
    16741 atgaggggtt tccgtgttgc tttacgaacc gctgtcgtta cctcggctgc tgaaatgggg
    16801 tcgagtccaa atgccatgcc accttgcttt acattggggc aggatatatt gagttctatc
    16861 atggggattt ctgtttcgtc gaggaggcgg gcgccttcga catagctttc tatcgtggag
    16921 cctgaaagat ttgcgatgct gacggtatca aatgagagca tggtttttaa ttgccgtttg
    16981 ataaaggttt cgatgccagg gttttctaag ccgatagaat tgataagacc actcgcggtt
    17041 tcgtgtaatc tttttcccgt gtttccctgt ctagcttcga gggtgagtcc ttttgagcaa
    17101 atgccgccga gtgcatctac attgacaagt tgtgcgtatt cttccccaaa gccaaaagtg
    17161 ccggaggcag cgatgacggg atttttaaag tggacaccgg ctatattgac tgagaggtcg
    17221 acaggattgt tcgcggattg ggagttttgg tttttatctt tttctttttc agatgtcctg
    17281 tgtgtattgc tatgtgtatc gatttgtgtt ttggaatgtg tattgttcat agtaaatcct
    17341 ttagaaaata agtttgtcgc cttgaaagat ggggccggct ttacagcatt gtctattgcc
    17401 ttctgttgtt tttatggtgc aaccgaggca ggctccgact ccgcagccca ttttttcttc
    17461 catgctaagc caggcttgta ctcctgcttc gagacagatt ttttgcaggt aggcgagcat
    17521 ggggagggga ccgcatgcgt agagcaaggt gtattttttt tccttgaggg tatcggctgt
    17581 taaaacagct gggagcattc catggacgcc gacacttccg tcatcggtgg tgatatagag
    17641 attttttgcc tgaacgtctt ctatgccgta ggttccagac ttaaagcttg cataaaaatc
    17701 gtagctttct ttttctaagc cttgtgcaaa acctgaaaca ggtgctatgc ctatgccccc
    17761 accaacgatg gcaactgagg gtggcagttg gttttcggta ttcgcctctt ttgccgcttc
    17821 tcgttcggca aaggctttgc tatttttcgc gccacttaat gattcgcttg gtttgaaaaa
    17881 atggtttccg agaggaccta tgagatctat cgataagcca acttgcaagt tgcaaaggtc
    17941 ttctgtgcct tcaccttttt gtagtatgag aaattcaagc tcaaggacat gcgcatcttg
    18001 gacggctgga aatgtttctt gtaggcagtc agcatggctt gcaagttctt tgccagatgc
    18061 atcgaggagg cgcactttat agacagaaat gggacgtgtt agcaaaacac gagaaggccg
    18121 agatttgagc atgtaaaatt gtccagcctt ggggattgat tgctctttgt gtttgagttc
    18181 tgtttcgacc ttgagagaaa aaacatcttt ttgtaattgt tttatttcac agacctttgt
    18241 tattgtttga ctttgtaata ctcgtcttct catgtgtacc tgctttagtt gtttatttgt
    18301 aaggattttt ttgcattaag caaatcttct tttgcatttt tggcggctct ttgagcgctt
    18361 tcgacaatgt cttttagaca gagtttttct gcacttcgtt tttcttctag taggctatca
    18421 tttttccaag cggttaaaat acctcgggat gagttcactg ttccaccggc cttatcaagt
    18481 agggtcgccg caattcgtgc tgctccgccc tgggcgccat agccggggat gaggaaaaag
    18541 atattggggt aggcgtcgcg caatgctctt gcatcgcttt cttcggtgca accgacgaca
    18601 gcgcctacgg gagagcaaac gtttgtgtta ttgctcgccg ttttggacaa gccagcatta
    18661 gcataaaggg atgtcatttc tttttcaatt gaaagaagtt tttcgccaac tctatcgaga
    18721 acaaagcctc cgcttgcaag tgcttgtttt tctatgtcga ccatgcctgg attagaggtc
    18781 cgtagcaaaa caaaaaaacc ctttggaagt ggggctgact tatccgtgga agctgttttt
    18841 tctctagcat ctggatttgt attattggtt tttggtttgc accattctaa aaaaggctcg
    18901 agggtatcaa aacccatgta ggggtttact gttatcgtgt tggcttcaaa gtctcctgaa
    18961 aagtgcgcgc gtgcataggc tttggcggtg tcggcaatgt cgcctcgttt tatatcggca
    19021 ataaagggaa tatcgagtga gcgaagatag cgaagtgttt caagatagca agaaagccct
    19081 ttgagtccca tcgcttcgta gtaggctatt tgcaccttgt agcagcctgt atcgtttttt
    19141 gttgcatcga tgagcgcttt gttataggca agaaccgcct ctgctggatg agcgtagtcg
    19201 ctcaaaatag attcaggaag ataggaggga tcggtatcga gaccgataca aagcggtcct
    19261 ttttctttgc aggatgcttg taacctgtgc atttcatgta aataattcat agcaagagaa
    19321 taacatagaa cgttttaaat aacaattctc attttacatt gcttagaaaa acattcaagc
    19381 tttgatcctt attttgttgt ggcgacccat atgccttccc aatttttttt ctcttcttct
    19441 gagaggggat tgttttcata aaaaataagt ttttcaagat atttttttgc gggcgggtca
    19501 gtcgctgcga tagaggtaaa gagttctttt gctgctaaaa aatctccgtc ataaaaggct
    19561 ttgagggctt tttcaaaaat atcgattgat tgttttttat tttcgtaggc tacttctgtc
    19621 atgggttcaa aaacgcgaac agcttgagat tttccgacaa ctgccgcctt cccgagctct
    19681 cgaaagcgca agcgacagcc atgtctttgt gcagcatttt ttgttgcttc tgagcacatg
    19741 gtatagctgt caaattgttt attaaatcct tcgaggcgcg ctgcaaggtt taccgaatct
    19801 ccaagcatag aataatcaaa gcgcgaatgt gagcccatgt ttccgacaac ggcgtctccg
    19861 gtgtttattc cgattctcat gagaaaaact tgcccagctc ttttttcaag ttcgggacgc
    19921 attgcatcga gcttgctttg gcacttgact gcggcttcca gggcggcttg tgcatgattt
    19981 tccaaatcga ggggtgcatt ccaaaaagcg attatagcat cgccttcgta tttgtcgatt
    20041 gtaccaccgg atgcaaggat aatatcggtc atttcgctta ggtagtcatt caaaagcgaa
    20101 gttaagtcgc ttggattgag tttttcagat atgcttgtaa aaccttgcac gtcagaaaaa
    20161 aagatgctta gatttcgcct ctcgccaccg agcttgagtt tgtctggatt ggcgatgagt
    20221 tgttcaataa cgatggggct taggtattgt ttaaaggcat tttttatata gcgccgttgt
    20281 tttccttctt gcgtgtagga gataatgacc gatattgcaa agcctaaaaa aagtgagaca
    20341 aagaagcttg caaggggaac gtagactccc ttataaaaga ggaaaaaata cacgaggctg
    20401 taagaggcaa taaggaagat aaaggagatc gttgtaagaa taccgctgag ttttgcatag
    20461 cgcgcatttg atgccaattt ctcgtgaata ccgattggaa gaatagaaac aaaggagctt
    20521 aggagaaggg caagggcaag aaggataaag ggaatttctt ttataaaaga gttttgcaaa
    20581 taattatcga gctgtgttac gtgaactccc accccgggat aggctgttga aatgggagtt
    20641 gcagtaatat caaataatcc aggggcaaaa aatccaaaga aaataaaact ttctgcaaac
    20701 tgttcggctt ccagtagggg agcatcgcct ttttggattg cataataact ttgcaaaata
    20761 tcggaggcgc tgtagggaat atatctatca agattttctt gaaagcgtaa aagctgtccc
    20821 ttgggatttt ctggtttgtc tgtttctacg gcaattcctg cagcgagaag gggagcaagt
    20881 ccgagactgg gtattttttc tccattataa gtggtaaaac tttttactcg tcgggcaacc
    20941 ttatcttcat cgcttgttcc tgttacgtcg cctaggaggg cagccgactg tcgaatagaa
    21001 ggtattggga ggagaggagg cttgttttta tcgctgtagt aaaaagtgtg tacaactttt
    21061 ccattttctc gacaagcttg tgcaaagttt tcatcgtctg cattgccata gagggagggc
    21121 tcggtaaaca aaacgtcgaa tgtaagggag gcagcatttg caaggttaaa ataacgtaca
    21181 atatccccat aagcttctcg aggccagggc caattccagc ctttttcttg aagagcccaa
    21241 tcgatacttt cttggtcaag caaaactaag gatatagcat cagatggatg cgtaaaagaa
    21301 gccgtcagtc gcattgcata gtcgtaaagc aaaaagtcta gccttgtggt aagcggtata
    21361 aaaaagctca gaatagatag gctaaaaaca aggatgctta taaacagaga gcgtagaaaa
    21421 acaggagcct tcttttttct tttcatcttt accctttttg tttacaaaaa aacagtttcg
    21481 ttaaagcgtt tatttcgatt tatagcctca ggacttgtcg catatttttt caaaacctta
    21541 tttacttcat taattctttg ctttgcagag gggtgtgttt ttgcaaaacc accagaggag
    21601 cgcggttgtt tttcttgtag tacctttagc atagaaatga ttgcgtgtgg attatatcct
    21661 gcatcaaaga gtagttttac cgctgcagca tcggcgtcgt attcttgttg tttagaatag
    21721 cccttgttta ccatggtggt aattacttcg tttatagatg catccatcat atctgcaagg
    21781 tccatgagat cttcgttccc agtaaggttg agaaataaac cacccgttgc aagcgctgct
    21841 tgtgagcttc ggcttgtttt tatagctttt aaactatgtt gaagctgtat atgtcctatt
    21901 tcgtgggcta taactgaggc aagggcatct tcattttctg cacaagagag caaaccacgt
    21961 gtgatgagga tatgccctcc agatgttgca aaggcgttta tttggtcgct atccaaaata
    22021 gaaacgtggt agccgttgta gagttctggc cgttcggaat gtataacaag agccatgcag
    22081 attttattta agtacgcggt tagcttgact tcatcagcga ggtcgtagga ttgtaaaaga
    22141 gttgctgcaa ccgaacgccc aatataatat tcctgctcag gagtgatggg ttctgcagcc
    22201 tttgcgattg ccatgccggc atctacgcca gaactaacaa cacgaccgag agtatcgaag
    22261 tcggagatgc tttcacagga aaaaaagaat gagagaagtg tgagacttcc gagtagtatt
    22321 acaagagatt ttgctttttt catccttcgc ctccatagag ctttccttca acaatgaatt
    22381 ctaataaatc ctcatccccg acatagttgt cttctatttt atccactaaa tcaaagcgta
    22441 attctccatg cctttgcttc atagcgtctt caacttcggc agaaaaacct tttccagcaa
    22501 gggcaagttc gctctcagaa agattttgcg tttttcccga aataatcttt ttctttgtaa
    22561 gatttgtgct tggtatccag cctttatttg aggggttttc ttttaattcg atctgcgtcc
    22621 acttacctgc ttcggcaaga ataataacag tatcgccgta atacacggtt cctagtttcg
    22681 atgcaaagaa tcctgtgccg cttttcaaaa tagattcttt cacatttaca tacattgtgt
    22741 cgcctgcttt tgcaaaaaca caaggaagca ggaagagaaa aattaaaaca atgctaacgt
    22801 gttttttcat aatgtctcct aaatctttag atactcagtg tatcatacta tagtactcat
    22861 ttctattata acacatgaaa gtgatgtttt atcaataaaa aacagtaaaa caacaaaaaa
    22921 ttattagagc tctgtaaatt gacaaaaaga ggcttaaaga gtactctttt tttatgtcag
    22981 aatccatcgt acaaaaaacc aaccctctgg gaacagaaaa agttggtacc ttgcttagaa
    23041 ccttttctat tccaagtatt atttccatgc tcgttggagc cttatacaat atagtcgatc
    23101 aaatctttat aggtcaaaaa ataggggagc tgggaaatgc cgcaacaaat gtcgcctttc
    23161 ccctcagtac cgcgagtgta gcaattgcgc ttcttatggg aattggggga gccgcagcct
    23221 ttaatctcgc aagtggacgt ggggaaacag aaaagggtgg atacatcatt ggcaactcgg
    23281 tgagcatcct cattattttg ggcattgttt taatgctcat aacccaggct tttttagaac
    23341 cgcttttgcg tttttttggc tcacctgaaa atgtgctcgg ctatgcaaaa acctataccg
    23401 gcattgtttc cttcggtttt ccttttttaa ttttatcaac tggggcgact caccttattc
    23461 gagctgacgg cagcccaaaa tacgctatgt tttgcaatct ttctggcgcg atattaaata
    23521 ctattctcga tcccattttt attttcgtcc tcgacatgga tatggcaggg gccgcaatcg
    23581 caacaattct tggacaaatg ctttcctttg tccttgctat aggctatctg cgaaaattta
    23641 gaacagtaaa acttgttttt tctcacttta ttccacgtgt gaagtatttt tcacaatcga
    23701 tgattttggg acttgctccg gcttttaatc aatctgcaat gatgcttgta caaattatta
    23761 tgaataagtc gctcgttttc tacggtgcct tgtcagccta tggcgctgca attcctcttg
    23821 cgagtgccgg cgttataaca aagacagcga tgattttctt ttctattatc ataggtattt
    23881 ctcaagggat gcaacctgtt gtcagtttta attacggggc aaaaaaatac gaccgtgtaa
    23941 aagaggctta taaaacctct cttaaggtag ccttgtttat atctattctt gcctttttac
    24001 tttttcagct tttcccacgg cagattgttt cgatttttgg aaagggatca gccgaatatt
    24061 ttgccttctc gacactctat tttcgcatct ttatgttttt tacctttata aactgtatac
    24121 aacctgtctc ggcaaatttt tttaccgcga ttggaaaacc gcataagggt atgtttttat
    24181 cccttacacg acaaattata tttttactac cgcttatatt tttgctgccg cttcgttttg
    24241 gcattgacgg ggtagtctat gcaggaccca tcgcagattt ttttgcagca agcgtttcag
    24301 tattcttcat aagttatgaa ctaaaaaaaa tgttataaat tgtgtaaaac aattgctttt
    24361 atctaaagaa aacagtatct ttaggtaaaa gtgctttttt ataaggattt aataatgata
    24421 aaaaaaattc ccctcttgtg tttctgcgtt ttgtttttca gttttatttt tggagaaacg
    24481 caggaagttc taaaactgag ccttgacgat gctgttttat tagcaatgca aaacaatgtg
    24541 cagctttctt ctatggcaat tgacttgcgt atgaaaaaag cggaaaaaga ctatgcctgg
    24601 aatgtgtttt tgccaagcat acaggcaact ggcactatgg cacgcacaaa cataaaagaa
    24661 gcgagcgcaa caattccttc tatgagtcca ccagggtttg caacaattac aacaaagctt
    24721 gatgaaaaag ataaatggaa ggtgatgggt ggtttgaata taagtttaaa cttaaatgct
    24781 gctctctttg aaggcttaag ggcaacgagg caatcttatg aagcaggctt gctgagctat
    24841 gaggcagcga ggcgcgacac cgagcaaaat gtaaaaaaag cattttacgg cttgctttta
    24901 caagagggaa gccttgctat atctaaagaa aaacttgcaa gcgcccaaaa gcgaaaaacc
    24961 caaatggaag caaacttcaa aaacggctta gtttctgagc ttcagtactt gcaagttcag
    25021 ctggcccttg aaacgcaaaa acctgcaatc atggaagcag aattattatt ggcacagcaa
    25081 aaagagctct ttgctttttt acttgggctg cccttgggaa cagagattgt cctcgtgggg
    25141 gagatagctc cgcatatcca agaattcaat gccgatgaac ttctcgcaga gtatttggga
    25201 cgctcctttg aactcgcaca acttgataaa aataaagaac ttatggaaac gcaattacgg
    25261 gcgacgctct tgcaaacata taccccgaca attgttttga gtcaaacctt taaccccgct
    25321 ctgggaaaaa ttgatgatga ttggtttaag aaagataact gggcagatac ttctggcgcc
    25381 tttagcctca cgatagcctt taatcttaca aatcttttgc ctttttctgg catggg
//
LOCUS       DAOSSW010000016        25331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_2138_length_25331_cov_5.338186, whole genome shotgun sequence.
ACCESSION   DAOSSW010000016 DAOSSW010000000
VERSION     DAOSSW010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 25331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25331
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2138_length_25331_cov_5.338186"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="PKW26_02630"
     CDS             <1..1364
                     /locus_tag="PKW26_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOQ92496.1"
                     /translation="RTNQDTCYHQRPLVEVGQKVEFASVLADGPATFNAELALGRNIL
                     VGFVPWHGYNYEDAILISQRVVKEDMFTSIHIKEFITEIRETKLGAEKITRDVPNTSE
                     KTLDNLDAEGIIRVGAKVKSSDILVGKVTPKSETETTPEFKLLNSIFGEKAKDVRDSS
                     LRVPHGIEGTVIDVQRLRRTEDDALNPGVEEVVKVLIATKRKLREGDKMAGRHGNKGI
                     VAKILPIEDMPYLEDGTPLDICLNPLGVPSRMNIGQILESELGLAAQFLDEWYESPVF
                     QSPTTEQIDGKLKEAGFNTNSKAILRDGMTGETFKNPVFVGVIYFMKLHHLVDDKMHA
                     RSTGPYSLVTQQPLGGKAQFGGQRLGEMEVWALEAYGAANTLQELLTIKSDDMTGRSK
                     IYESIVKGEPSTAAGVPEAFNVLVQELRGLALDFTIYDAKGKQIPLTERDDELITKAG
                     SNF"
     gene            1431..>1894
                     /locus_tag="PKW26_02635"
     CDS             1431..>1894
                     /locus_tag="PKW26_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOQ92497.1"
                     /translation="MRDIQDFDNLMIKLASPETIRAWSYGEVKKPETINYRTLRPEKE
                     GLFCEKVFGTTKEWECYCGKFKSIRYKGIICDRCGVEVTHFKVRRERMGHIELAAPVS
                     HIWYYRSTPSRMGLLLDLQVAALRSILYYEKYIVIDAGDTDLKQMQLLTEDE"
     assembly_gap    1895..1994
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1995..5540
                     /gene="rpoC"
                     /locus_tag="PKW26_02640"
     CDS             <1995..5540
                     /gene="rpoC"
                     /locus_tag="PKW26_02640"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOQ92498.1"
                     /translation="PVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLQQLQ
                     APEIIIRNEKRMLQEAVDALFDNSKRKKAVKGAANRPLKSISDMLKGKQGRFRQNLLG
                     KRVDYSGRSVIVVGPELKLWQCGLPTKMALELFKPFIMKKLVDKDIVFNIKKAKTLVE
                     QEAPEVFAILDEVVREHPVMLNRAPTLHRLGIQAFEPVLVEGKAIKLHPLVCHAFNAD
                     FDGDQMAVHVPLTQAAQMECWTLMLSARNLLDPANGKTIVYPSQDMVLGIYYLTKVKP
                     SGKGTGKRFSSFNEVMMAVEQDNIEYQSLIKVPVQKAPHNGAIVETTPGRLLFNDQMP
                     EEVPYVNELLGDKELKNFIETVHKERGSWLTVQMLDTIKDIGYKYATFFGATISMDDI
                     IIPEEKKGMMEKANKEVVSIMQQYHKGVITQEERYNRVIEVWQKANDELTSVMMKTLE
                     KDQEGFNSIYMMAVSGARGSKNQIRQLSGMRGLMAKPSGEIIELPIRANFKEGLSVIE
                     FFISTNGARKGLADTALKTANAGYLTRRLVDIAQDVVINEDDCSTINGIAYSAIKDGD
                     DILEALHDRIVGRFTLERVLHPITREQICAANEYITDEKAVEIENAGVESVKLRTVLT
                     CESKHGVCCKCYGRNLAHNKIVEIGEAVGIIAAQSIGQPGTQLTMRTFHVGGTATKTT
                     EENRTVLKYPVVVNSISGTHVEMPEGWLFTRKGQMLVNRIIERHEFSEKDTLMVEDGG
                     RVGVGDVLFKKGTKEVKSSVIAFVTIRDNVIYLTGNEQKIEIKNGSQMQVVAGDIVEA
                     GQTIASFDPFAEPIIAEVDGYVHFEDIIHGSTLDEEIDEETGNVERIISDSYVETKQP
                     RILITDEAGNERGSYYLPTGSYLQVEDKAPISAGTIIAKILKESAKTRDITGGLPRVE
                     ELFEARKPKNPAVLAQISGTVHFKGIAKSKRIVVVQDKYGKNFEHQVPINKRLLVRDG
                     DEVIAGDQLSDGDLNPHDILSISGENALQNYLMNEIQQVYRVQGVSINDKHIGVIIRQ
                     MLRKVELVAVGDTRFIYGQQVDKYQFHEENRRVISEGGQPAIARPMFQGITKASINIG
                     SFISAASFQETTKVLTNAAVAGAQDSLRGLKENVIIGHIIPAGSGIRNYRNIKLFDDK
                     AVDLDVQMEEILEKRKLEKALEVEMDEGNLGADMN"
     gene            5665..6038
                     /gene="rpsL"
                     /locus_tag="PKW26_02645"
                     /pseudo
     CDS             5665..6038
                     /gene="rpsL"
                     /locus_tag="PKW26_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012538144.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
     gene            6051..6521
                     /gene="rpsG"
                     /locus_tag="PKW26_02650"
     CDS             6051..6521
                     /gene="rpsG"
                     /locus_tag="PKW26_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOQ92499.1"
                     /translation="MGRKNKTINRPILPDAKYNSPVITKFVTRMMLDGKKATNVAIIY
                     EAMDKLKEKTDKDPLEVFLKALDNVKPMVEVKSRRVGGATYQVPIEVRESRREALAMR
                     WIITAARAKSGRGMGERLANELLDAYNNTGTAYKKKDDTHKMAEANKAFAHFRW"
     gene            7120..8307
                     /gene="tuf"
                     /locus_tag="PKW26_02655"
     CDS             7120..8307
                     /gene="tuf"
                     /locus_tag="PKW26_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOQ92500.1"
                     /translation="MAKEKFVRTKPHMNVGTIGHVDHGKTTLSAAITKHSAQKYGDKA
                     LNYDEIDNAPEEKARGITINTRHLEYQSDKRHYAHIDCPGHADYVKNMITGAAQMDGA
                     ILVVSAPDSVMPQTKEHILLARQVGVPGMIVFLNKVDLLDDPDLLELVEEEVRDVLEQ
                     YQFPRDTPIIKGSAFKALADGASAEDTAPIDELLDTMDSYFADPVRAVDLPFLMPVED
                     VFSIPGRGTVVTGRIERGVINMNEEVEIVGIRPSQKTVITGIEMFNKLLDKGETGDNV
                     GLLLRGIEKTAVERGQVLAKTGSIKPHTKFEGQVYVLSKEEGGRHSPFFSGYRPQFYF
                     RTTDITGTVYLPEGVDMIKPGDNTKIIGELINPIAMDKGLKFAVREGGRTIASGQVTE
                     ILA"
     gene            8361..8669
                     /gene="rpsJ"
                     /locus_tag="PKW26_02660"
     CDS             8361..8669
                     /gene="rpsJ"
                     /locus_tag="PKW26_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOQ92501.1"
                     /translation="MAAEKIRVRLRAFDVDLIDQSAKSIVQTVQKAGAKVAGPIPLPT
                     RINKVSVLRSPHVNKKSREQFEMRTHKRLIDIIEPSSDVIDSLMKLELPAGVDVEIKQ
                     "
     gene            8736..9362
                     /gene="rplC"
                     /locus_tag="PKW26_02665"
     CDS             8736..9362
                     /gene="rplC"
                     /locus_tag="PKW26_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOQ92502.1"
                     /translation="MKGLIAKKVGMTQVFDESGNLTPVTVIRVEPNTVVALKDKETFG
                     YDAVVLGLDDLRKSRITKPYAGQFPEGLAPKQKIKEFRDFEAEVKVGDEVGLELFEGI
                     RFVDVTGISKGKGFQGVMKRWNFHGGRASHGSKFHREAGGTGQCTSPGRTFKNTKQAG
                     RMGREQVTVQNLRIVKIDPELKVVMVRGAVPGNRDTTLIIKSAVKKEF"
     gene            9381..10025
                     /gene="rplD"
                     /locus_tag="PKW26_02670"
     CDS             9381..10025
                     /gene="rplD"
                     /locus_tag="PKW26_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOQ92503.1"
                     /translation="MEKKVYSTDGKELRAITLDDKVFGLPINEDVIYYAINNELANKR
                     VGTACTKGRAEVNGSNAKPFKQKGTGGRARRGDKKSPLLRGGGVIFGPKPRDFSFVLP
                     KKVKRLAMKSILSLKAQTDRLTIIEDFTIESGKTKDLVSILKNFVKDEHRTVVVLKDN
                     DAMLKRAGKNIPTVSFLSYNRLRAHDLFYGQKIIMLESAVKNLSDFYVEEQEAK"
     gene            10025..10309
                     /locus_tag="PKW26_02675"
     CDS             10025..10309
                     /locus_tag="PKW26_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOQ92504.1"
                     /translation="MMLEDILVEPVLSEKATLLREEGKYVFKVHPKANKYEIKDAVQK
                     LFNVKVVDCTVMNVFGKMKRVRYRTGRTSSWKKAIVKLAPGETIKVFEGV"
     gene            10339..11169
                     /gene="rplB"
                     /locus_tag="PKW26_02680"
     CDS             10339..11169
                     /gene="rplB"
                     /locus_tag="PKW26_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOQ92505.1"
                     /translation="MALKVYKPITAGTRARIDLVRDEITTNRPEKSLTTGKKRNAGRD
                     ANGRISVRHKGGGHKRRYRDIDFKRDKHGVPGTVRTIEYDPNRSANIALIFYADGEKR
                     YIIAPKGLEVGQKILSGESASPTVGNALPLEAIPVGFTVHNIELTIGKGGQLARSAGT
                     SALVAAKEGDYVTIRLPSSEVRLVHKKCYATIGTVGNEDRMNTQLGKAGRNRWLGVRP
                     GVRGMSMNPVDHPLGGGEGRGKGRNPVTPWGQPCKGFKTRKKRKVSSNFIVSRRKKKR
                     "
     gene            11179..11460
                     /gene="rpsS"
                     /locus_tag="PKW26_02685"
     CDS             11179..11460
                     /gene="rpsS"
                     /locus_tag="PKW26_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOQ92506.1"
                     /translation="MSRSVKKGPFIEKSLYKKVIEMNKAADRKMIKTYSRCSTIIPEM
                     VGNTISVYNGKSWVPVYITENLVGHKLGEFSPTRVFRGHAGSDNKAGRR"
     gene            11464..11820
                     /gene="rplV"
                     /locus_tag="PKW26_02690"
     CDS             11464..11820
                     /gene="rplV"
                     /locus_tag="PKW26_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOQ92507.1"
                     /translation="MAEKKDYIAKTKFLIASPTKVRPVANVVKRKNCSEAMAILENMP
                     QKGATLIKSTMKSAIANALYANKKLDEEMLYVKDVQIDEGPRLKRIWYRARGRADMLK
                     RRMCHITVVVAEKAGN"
     gene            11822..12583
                     /gene="rpsC"
                     /locus_tag="PKW26_02695"
     CDS             11822..12583
                     /gene="rpsC"
                     /locus_tag="PKW26_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOQ92508.1"
                     /translation="MGQKVHPIGLRLGINKTWQSRWYADPREYADLLHEDLKIRKMIS
                     TMPECKNADIAEVEIIRHPQRVTIVIHTARPGVIIGVKGANIEKIGAEIQKKIAKKVQ
                     IKIKEIKRSEINASLIAQNIARQLVGRVSFRKVLKQSIANAMRGGAQGVKVRLSGRLG
                     GAEMSRTEEHKDGRVPLHTLRANIDFGFAEAHTTFGKIGVKVWVYNGMMYGYEQNEDA
                     GQVQRKRRERTDRAEGADRDSRPARPSRSAKAPRS"
     gene            12587..13006
                     /gene="rplP"
                     /locus_tag="PKW26_02700"
     CDS             12587..13006
                     /gene="rplP"
                     /locus_tag="PKW26_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOQ92509.1"
                     /translation="MALSPKRVKHRKVQRGRIKGNATRNCNVDFGDIALVALEPFWLT
                     AQQIEAARVALNRRINRKGQVWIRVFPDKPYSKKPAETRQGKGKGAPDHWTAVVKPGQ
                     VLFEIGGVELDTAAQAIILAGSKLPFKTKVAYKQDVQ"
     gene            13018..13218
                     /gene="rpmC"
                     /locus_tag="PKW26_02705"
     CDS             13018..13218
                     /gene="rpmC"
                     /locus_tag="PKW26_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOQ92510.1"
                     /translation="MAKKEKDLTYSELIVKRNELKKKYMDLRFQMVIGHVDNPVQKRT
                     MRRDIATLNTLIRQKEIAGLDK"
     gene            13229..13501
                     /gene="rpsQ"
                     /locus_tag="PKW26_02710"
     CDS             13229..13501
                     /gene="rpsQ"
                     /locus_tag="PKW26_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOQ92511.1"
                     /translation="MEEQVVQGKSSKRSFVGVVTSDKMDKTIVVQISTKKLHKLYKKY
                     VTSVKKYKAHDEKNDARIGDRVKIVESRPLSKDKCWRLVEVLERAK"
     gene            13512..13880
                     /gene="rplN"
                     /locus_tag="PKW26_02715"
     CDS             13512..13880
                     /gene="rplN"
                     /locus_tag="PKW26_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOQ92512.1"
                     /translation="MIQMQSYMNVADNSGAKVVQCIKVIGGSKRRYASVGDVVVVAVK
                     ETLPTSTIKKGTIEKAVIVRISKEYRRSDGTYIRFDDNACVIIDANKNPKGKRVFGPV
                     ARELRDMDYMKIVSLAPEVL"
     gene            13903..14217
                     /gene="rplX"
                     /locus_tag="PKW26_02720"
     CDS             13903..14217
                     /gene="rplX"
                     /locus_tag="PKW26_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOQ92513.1"
                     /translation="MDKKFKIRKDDTVEIIAGKDKGKRGPIVKILRSKDRVIISGANL
                     VKKAMKKRSQQDKGGIVEVEAPIHVSNVAIVCKKCGPTRIGFKLDGEKKTRVCRKCGD
                     TL"
     gene            14217..14768
                     /gene="rplE"
                     /locus_tag="PKW26_02725"
     CDS             14217..14768
                     /gene="rplE"
                     /locus_tag="PKW26_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOQ92514.1"
                     /translation="MSNYVPRLKKVYKDTIAPELFKELGCTSVMQVPAVKKVVVSMGV
                     GEALANKKLLDAAVTDLTTITGQKAVKTKAKKSIANFKLREGNEVGVMVTLRGTQMYE
                     FLDRLINVALPRVKDFRGIKATGFDGRGNFSLGVTEQIIFPEIDFDKIEKISGMNINI
                     VTSAQTDSDARALLSKFGMPFRK"
     gene            14779..14964
                     /locus_tag="PKW26_02730"
     CDS             14779..14964
                     /locus_tag="PKW26_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002557082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOQ92515.1"
                     /translation="MAKKSLIIKASRPQKYSTRKYNRCLVCGRPRGYLRKYKMCRVCF
                     RKLASEGLIPGVTKSSW"
     gene            14978..15376
                     /gene="rpsH"
                     /locus_tag="PKW26_02735"
     CDS             14978..15376
                     /gene="rpsH"
                     /locus_tag="PKW26_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOQ92516.1"
                     /translation="MSASDPIADMLTKIRNAALARHEKVDVSASKIKLEIVKILKTEG
                     YIKNFKKISQDGMNSIRIFLKYDDKNASVIKGIQKISTPGRRVYSGYKDLPRVYNGYG
                     TVILSTSLGVTTGRKANEKMVGGELICTVW"
     gene            15389..15925
                     /gene="rplF"
                     /locus_tag="PKW26_02740"
     CDS             15389..15925
                     /gene="rplF"
                     /locus_tag="PKW26_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOQ92517.1"
                     /translation="MSRIGKLPVQIPAGVKVSVSANEISVEGPKGKLSQDFNNLVSIK
                     VEGTDVVVNRANDSKPARSAHGLYRSLVENMIQGVTEGFSKTLVINGVGFRAEVQGNL
                     LMLNLGYSNDIYAGIPENLTVVVEAQGKLTISGIDKQAVGEYAAQIRKLRLPEPYKGK
                     GIRYSDEVIRRKVGKSGV"
     gene            15938..16300
                     /gene="rplR"
                     /locus_tag="PKW26_02745"
     CDS             15938..16300
                     /gene="rplR"
                     /locus_tag="PKW26_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOQ92518.1"
                     /translation="MHKKRNEKDRKRLKRKIHIRKRLHGTADRPRMTVFRSNKNLFVQ
                     VIDDEQGKTLASISTLEKEFSSMKATVKDAEKLGEAMGKRLVENKINTVVFDRNGYLY
                     HGVVKSLADGARKAGIVF"
     gene            16309..16821
                     /gene="rpsE"
                     /locus_tag="PKW26_02750"
     CDS             16309..16821
                     /gene="rpsE"
                     /locus_tag="PKW26_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOQ92519.1"
                     /translation="MYNQRNYDKDQPKEFVEKLVKLNRTAKVVKGGRRFSFSALTVVG
                     DKKGRVGFGFGKANDVSEAIRKSIEKAKKNMVVLPLKNGTIPHKIQAKYKGSSVLLLP
                     ACSGTGIIAGGPVRAVLEAAGATDVMSKSLGSNTSINVVQATFAAIDGLMDARVIAKN
                     RGKALKDVWG"
     gene            16824..17015
                     /gene="rpmD"
                     /locus_tag="PKW26_02755"
     CDS             16824..17015
                     /gene="rpmD"
                     /locus_tag="PKW26_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOQ92520.1"
                     /translation="MAKNKRIKITLVRSTIGQKAPKVATVRSLGLKKLNSTVEHEVNP
                     AIMGMVAAVSHLVTYEELN"
     gene            17015..17467
                     /gene="rplO"
                     /locus_tag="PKW26_02760"
     CDS             17015..17467
                     /gene="rplO"
                     /locus_tag="PKW26_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOQ92521.1"
                     /translation="MSDFTLKAPVGANKAKKIVGRGSGSGLGTTAGRGNKGQQSRSGG
                     GTYVGFEGGQMPLYRRIAIRGFSNYPFKKEYALFNLRDLEAKYENGETVSKETLIKKG
                     LLKKAGILVKVLGDGELTKKLTVNVDKVSASAKEKIEKIGGTVAISNE"
     gene            17481..18800
                     /gene="secY"
                     /locus_tag="PKW26_02765"
     CDS             17481..18800
                     /gene="secY"
                     /locus_tag="PKW26_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOQ92522.1"
                     /translation="MANNPIVNMFRVKELRERILFTFIVLAVYRFGGVLTIPGISAQA
                     LTTYFSSLTKGNAFVDYMNFFAGGAFSKFSLFMLGVTPYISTQIIMQLALIIFPSLKK
                     MVQEDGGQKKFQTWTRIGTVFVSLIQSLAVVAYAYSIPNAVLIQNIWLFRAIAMVSIT
                     TGTMATIWLGEQITSRGIGNGVSMLIFAGIVSRLPSAVWELGGLVKNNEINVVFVVVV
                     LLMFVAVVALVVYEQQGQRKIPVHYAKRVVGRKMYGGQNTYIPFKLNPSGVIPVIFAS
                     SFLTFPLQLATSFGVKYRWLARLANFLTPSGWWYNIIQVLLIVFFAYFYTQVTLNPTE
                     IAKNIRENGGSIPGIRTDKMEEYMQKVLNRLMLPGSLYLAFIAVIPTIIQNLFGFPHS
                     ISSIIGGTSLLILVGVDLDTMSQVEALLKMHHHDGLVKKGKLRSRNL"
     gene            19044..19157
                     /gene="rpmJ"
                     /locus_tag="PKW26_02770"
     CDS             19044..19157
                     /gene="rpmJ"
                     /locus_tag="PKW26_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOQ92523.1"
                     /translation="MKVRTSVKPICDKCKVIKRFGVVRIICTNPKHKQKQG"
     gene            19179..19544
                     /gene="rpsM"
                     /locus_tag="PKW26_02775"
     CDS             19179..19544
                     /gene="rpsM"
                     /locus_tag="PKW26_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOQ92524.1"
                     /translation="MARIAGVDLPNKHVNIALTYIYGIGRSSADEICEKTKIDPNRMM
                     NDLSQDEVGVIRTLIDAEYKVEGRLRSETGLNIKRLMDIGCYRGLRHRKGLPVRGQRT
                     RTNSRTRKGRKKTVAGKKK"
     gene            19567..19947
                     /gene="rpsK"
                     /locus_tag="PKW26_02780"
     CDS             19567..19947
                     /gene="rpsK"
                     /locus_tag="PKW26_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOQ92525.1"
                     /translation="MATVKRRKEKKSVYEGNVYIQATFNNTIVTITDLKGNALSWASS
                     GGLGFRGAKKSTPFAAQTVAETAVQKAVSYGLREVHVYVKGPGVGRESAVRSLGALGL
                     KVKSISDVTPIPHNGCRPRKTRRM"
     gene            19965..20600
                     /gene="rpsD"
                     /locus_tag="PKW26_02785"
     CDS             19965..20600
                     /gene="rpsD"
                     /locus_tag="PKW26_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOQ92526.1"
                     /translation="MGRYNGPVCRLCRTEQRKLYLKGDRCNSDKCPLNKKRGAPGKDP
                     KARIGKRSEYGLQLREKQKLKRMYGMFEKQFHLTFEKANRMSGVTGENLIILLESRLD
                     NFVYRMHLAASRSQARQLVSHGHILVNGKPVNIPSYHLKPSDVVEVKEKSKKLVSILD
                     SLKEVSKSGVSSWIDLDIDAQKATFVSYPSREEVADLADIKEQLIVELYSK"
     gene            20610..21665
                     /locus_tag="PKW26_02790"
     CDS             20610..21665
                     /locus_tag="PKW26_02790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOQ92527.1"
                     /translation="MARKNLLKGFKKPKGINFEHIDVNNHYGKFSAFPFETGFGTTVG
                     NTLRRVLLSSIQGYAVSSVRITSFDADAVPHVISSEFEAIPNVVEDTLEILNTLKQVR
                     LRLPEDIEQDTFTFEFKGPGKVTSDMLEKGGSLEVFTKDMHLFTMMENAHIEFVVQVD
                     LGRGYVPAEINENYIEFVNTIPMDAIFTPVTRVQYSIEPCRVGQRNDYDKLIIEIWTD
                     GTISPEDALAEGAKIAKDHFAIFINFNEDDDHKQDDQDEADERIRKILNTPVEELELT
                     VRSSNCLKNANIRTIGELTKKSEEDIAKTRNFGKKSLAEIKEKLQEWNLSLGMTDYSH
                     LRAKVDLAASKEESDES"
     gene            21655..22197
                     /gene="rplQ"
                     /locus_tag="PKW26_02795"
     CDS             21655..22197
                     /gene="rplQ"
                     /locus_tag="PKW26_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOQ92528.1"
                     /translation="MNHKMGFNALSRTSAHRRAMTRNMVTSLFRFERITTTKAKALEV
                     RKTAEKLITRSKVDSVHNRRQAARFIQDKIILAKLFTDIGPRMKDRAGGYTRVLKLGF
                     RQGDAADMVILELVDFSLDGDTKPASKSEKKDVKKANADTKAKVDTKSKTKAKTTEKA
                     EDLSKATAAEPSTENEKTEE"
     gene            22207..22389
                     /locus_tag="PKW26_02800"
     CDS             22207..22389
                     /locus_tag="PKW26_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92529.1"
                     /translation="MSKSFRGRNLRELPASGRGLCAQCKREGVKILHEVEIDGAKNKI
                     CKICKATNKNKANKAS"
     gene            22579..23715
                     /locus_tag="PKW26_02805"
     CDS             22579..23715
                     /locus_tag="PKW26_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ92530.1"
                     /translation="MKKVIKTILIALFTLMFFIGCSKEDTSVIKIGGIFPLSGDVAVY
                     GVEAKNGIELAILEINAKGGVNGKQLVLVSEDDEGSPEKTVNAFKKLTSKDKVKIVIG
                     SLTSGCTAAITTLAQNQKILLIAPAATMESITDAGNYVFRACFIDPFQGTVGGKFAAE
                     ILDSKRAAVLYDTDNDYSVGLYENFKKSFVANGGSMAAEESYTSGDVDFNAQITKIKS
                     ANPDVVYLPDYYATVSLIAKQLRAQDIHTPIVGADGWGGLVDNAGDEVLNGFYSDHYA
                     ADSTDDIVVSFVNAYKDRYKSTPVSFAALGYDCVYILKDAMERAGSEEASIVRNEVEK
                     TNGAYVTGQLTFDAKRNPIKSAVMMEIVKQDSVLTPVYKATVNP"
     gene            24070..24969
                     /locus_tag="PKW26_02810"
     CDS             24070..24969
                     /locus_tag="PKW26_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOQ92531.1"
                     /translation="MDTISMVIQQLINGISLGSIYALIALGYTMVYGIVKLINFAHGD
                     ILMVGAYVGFFVLSAFGTSPFGFFVAIIISMVVCALFAIVIERFAYRPLRNAPRLNSL
                     ITAIAMSLIIQNAARVIPFIGPNPRQFPTLAKSTVYIGSFSLSNIQLIVIALSAILML
                     ILAYIIKFTKIGKALRAVSFDNKAASLMGISVNKTISFTFALGAALAAVGGVLYATAY
                     PQITPTMGATPGLKAFIAAVLGGIGSIPGAMIGGYILGLAETFAKGFISSQFANAISF
                     AILIIILLVKPTGLMGKNEREKV"
     gene            24972..>25331
                     /locus_tag="PKW26_02815"
     CDS             24972..>25331
                     /locus_tag="PKW26_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOQ92532.1"
                     /translation="MQNIKMFNKKMIFPLIVSVFLLIVPTILIKLAIIDAYFAQIITL
                     AAINAIMAISVNIICGFTGQLSLGQASFMAIGAYMSILLTEILGLPMGLSIILAGFSA
                     AFVGFLIGFPTLKLRGDY"
BASE COUNT         8265 a         4332 c         5540 g         7094 t
ORIGIN      
        1 aaagaaccaa tcaagatacc tgttatcacc aaagacctct tgtggaagtt ggccaaaaag
       61 tggagttcgc aagtgttctt gctgacggtc ccgcgacctt caatgctgaa cttgcacttg
      121 gtcgaaacat acttgttggt tttgtacctt ggcacggcta taactatgaa gatgctatct
      181 taatttcaca acgcgttgtt aaagaagata tgtttacttc aatacatatt aaagaattta
      241 ttacagaaat acgcgagaca aaactcggcg cagagaaaat aacccgcgat gtacccaata
      301 caagcgaaaa aacacttgac aacttagatg ccgaaggtat tatccgtgtt ggtgctaagg
      361 ttaagtcttc cgatattctc gtcggtaagg ttactccgaa gagcgaaaca gaaactactc
      421 cagaatttaa gcttttgaat tctatctttg gtgaaaaagc aaaagacgtt cgagattctt
      481 ccttacgtgt tccacacggt atcgaaggta cggtaatcga tgtgcaacgt ttacgcagaa
      541 ctgaagacga tgctttaaat cctggcgttg aagaagttgt aaaagtctta attgcaacca
      601 agcgaaaact tcgcgagggc gataagatgg ctggacggca tggaaacaaa ggtattgttg
      661 cgaaaatcct tcctatagaa gatatgccat atctagaaga cggcacgcca ctcgacattt
      721 gtttaaatcc gcttggagtt ccatctcgta tgaatatcgg acagattctt gaaagcgagc
      781 ttgggcttgc agcacagttt ttggacgaat ggtacgaatc tcccgttttc cagtcaccaa
      841 ctaccgagca aattgatgga aagcttaagg aagcaggatt taataccaac tccaaagcga
      901 ttttgcgcga cggtatgaca ggcgaaacct tcaaaaaccc tgtttttgtt ggcgttattt
      961 actttatgaa actgcatcac ctcgttgatg ataaaatgca cgctcgttca acaggtcctt
     1021 attcactcgt aacgcaacaa cctcttggtg gtaaagcaca gtttggtgga caacgtttag
     1081 gagaaatgga agtatgggca ctcgaagcct acggagctgc gaacaccttg caggaattac
     1141 ttacgattaa gtcagatgat atgaccggtc gctcaaaaat ttacgagtca atcgtaaagg
     1201 gtgaaccttc aacagccgca ggtgtaccag aagccttcaa tgtattagta caagaattgc
     1261 gtggacttgc cttagacttt actatctatg acgcaaaggg caagcaaatc ccgcttacag
     1321 agcgtgatga cgagcttatt acaaaggctg gttcgaactt ttaacgagaa ttagagcacg
     1381 tgaacagtga gtagttttgc tattgctgtt cacaacaagt aggagaacat atgcgagata
     1441 tccaagattt tgataacttg atgattaaac tagcttcgcc agaaaccatc agagcatggt
     1501 cctatggtga agtaaaaaaa ccagaaacaa taaattatcg aacattacga ccagaaaaag
     1561 aagggctttt ttgcgaaaag gtctttggta ctacaaaaga atgggaatgt tactgcggta
     1621 aatttaagtc tattcgatat aagggtatta tctgtgatcg ttgtggcgta gaagttacac
     1681 actttaaagt gcgacgcgaa agaatgggac atattgaact cgcagcccca gtttcacata
     1741 tatggtatta tcgttctact ccttcccgca tgggactttt gctcgacttg caagttgcag
     1801 ccctacgttc tatcttatac tacgaaaaat acatcgtaat cgatgcaggg gataccgacc
     1861 tgaagcaaat gcaattgctt acagaagatg aattnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnncctgtt attccaccag aattgcgccc catggtgcag ctcgacggcg
     2041 gtcgctttgc aacatcagac ttaaacgatt tatatagaag agttattaac cgaaacaatc
     2101 gattaaagag gttgcagcag ttacaagctc ctgaaattat tattcgaaat gaaaaacgca
     2161 tgctccaaga agctgttgat gcgcttttcg ataactcaaa acgaaaaaag gcggtaaaag
     2221 gggcagcaaa ccgaccgctc aagtctattt ccgatatgct caagggaaaa caagggcgat
     2281 tccgccaaaa ccttcttggg aagcgtgttg actactccgg tcgttcggtt atcgttgttg
     2341 gccctgagct caaactttgg caatgtggtt tacctacaaa gatggcctta gagcttttca
     2401 agccctttat aatgaaaaaa ctcgtcgata aagacattgt tttcaatatt aaaaaagcaa
     2461 aaaccctcgt tgagcaagaa gctcctgaag tttttgctat tctcgacgaa gttgtgcgcg
     2521 aacatcctgt tatgcttaac cgagcgccta ccttgcatag acttggaatt caagcctttg
     2581 aaccagtact agtagaaggt aaggcaataa agcttcatcc tcttgtttgt catgctttta
     2641 acgctgactt tgacggtgac caaatggctg ttcacgtgcc tctcacccaa gctgcgcaaa
     2701 tggaatgctg gacactcatg ctttcagcgc gaaaccttct tgacccagcg aacggaaaga
     2761 ctattgtgta tccatcacag gatatggttt taggtattta ttatctaacg aaggttaagc
     2821 caagcggaaa agggacagga aagcgtttct cttctttcaa tgaagtgatg atggctgttg
     2881 agcaagacaa tattgagtat cagtctctta ttaaggtgcc agtacaaaaa gctccacata
     2941 atggggcaat tgtcgaaact accccaggac gacttttgtt taacgaccaa atgccagaag
     3001 aagttcccta tgtaaacgag cttttgggcg ataaagaact aaaaaacttt atagaaactg
     3061 tgcataagga aagaggttct tggttaactg tgcaaatgct cgacacgatt aaagatattg
     3121 gttataaata cgcaactttc tttggcgcga cgatttctat ggatgatatt attatccctg
     3181 aagagaaaaa aggtatgatg gaaaaggcaa ataaagaagt tgtttccatt atgcagcagt
     3241 accacaaggg tgttatcaca caagaagagc gatataaccg agttattgag gtttggcaaa
     3301 aagcaaacga tgagcttacc tcagtgatga tgaaaacgct cgaaaaagac caagaaggtt
     3361 tcaatagcat ctatatgatg gctgtttctg gagcacgagg tagtaaaaac caaatccgac
     3421 agctctctgg tatgcgtggt cttatggcaa aaccaagtgg tgaaattatt gaacttccta
     3481 ttcgagcaaa ctttaaagaa ggcttatccg ttatagaatt ctttatttct acaaacggtg
     3541 ctcgaaaagg tcttgctgat acagcgctaa aaaccgcaaa cgccggatac cttacgcgac
     3601 gtctcgttga tattgcacag gacgttgtta taaacgaaga cgattgtagt accattaacg
     3661 gtattgcata ttctgcgatt aaagacggtg atgatattct cgaagctctc cacgatagaa
     3721 ttgttggacg atttactctg gagcgcgttt tgcaccccat tacacgggaa caaatttgtg
     3781 ctgcaaacga gtacattacc gatgaaaaag ctgttgaaat agaaaacgcc ggtgtagaaa
     3841 gcgttaaact tcgaactgtt ttaacctgtg agtctaaaca cggtgtttgt tgtaagtgtt
     3901 atggacgaaa ccttgctcat aacaagattg tcgaaatagg cgaagcagtt ggtattattg
     3961 cagctcaatc tatcgggcaa ccgggaacac agcttacgat gagaaccttc cacgttggag
     4021 gaaccgcaac aaagactacg gaagaaaaca gaactgtatt aaaatatcct gttgttgtaa
     4081 acagtatctc tggaactcac gtagaaatgc cagaaggttg gctctttact agaaagggac
     4141 aaatgcttgt aaaccgtatt atcgaacgtc atgagtttag tgaaaaagat acactcatgg
     4201 tagaagatgg tggaagagtt ggtgtaggcg atgtgctttt caaaaaaggc acaaaagaag
     4261 tgaaatcctc cgttatagcc tttgttacca ttcgtgataa tgttatctat ctgacaggaa
     4321 atgaacaaaa gatagaaatt aaaaacggct cacaaatgca ggttgttgca ggagatatcg
     4381 tagaagctgg acaaacaatt gcctcttttg acccctttgc tgagcccatt attgccgaag
     4441 ttgacggtta cgtacacttt gaagatatta tccacggttc aacactcgat gaagaaatcg
     4501 acgaagaaac aggaaatgtt gagcgtatta tttcagattc ctatgttgaa acaaagcaac
     4561 ctcgtatcct tataactgac gaggctggaa atgaacgcgg aagttattat ctaccaacag
     4621 gttcgtatct tcaggtagaa gataaggcgc cgattagcgc tggaaccata atcgcaaaaa
     4681 ttctcaaaga atctgcaaaa acacgcgata ttacgggggg actacctcga gttgaagagc
     4741 tttttgaagc aagaaaacca aaaaaccctg ctgttttagc acaaatttct ggaacagtgc
     4801 atttcaaagg cattgcaaag agtaagcgga ttgtggttgt tcaagataaa tacggcaaaa
     4861 actttgagca tcaagtacca atcaataagc gattgttagt gcgagatggt gatgaagtta
     4921 tagctggtga ccaattgagc gatggtgact taaacccaca tgatattttg tcaatttctg
     4981 gtgaaaatgc ccttcaaaat tatttgatga atgagattca gcaagtgtat cgtgtacaag
     5041 gtgtaagtat caatgacaaa catatcggtg taattatccg gcaaatgctt cgaaaggttg
     5101 agcttgtagc cgttggtgat actcgcttta tctacggaca acaggttgat aaataccaat
     5161 tccatgaaga aaatcgccgt gttatttcag aaggtggaca acctgcgatt gcacgcccga
     5221 tgtttcaagg tataacaaag gcttcgataa acattggctc ttttatttcc gcagcttctt
     5281 tccaagaaac tacaaaggtt ttgacgaatg cagcagttgc tggagcgcaa gacagtctcc
     5341 gcggattaaa agaaaatgtc attatcgggc atattattcc tgcaggttct ggtattcgaa
     5401 attatcgcaa tattaaactt tttgacgata aagcggttga ccttgacgtg caaatggaag
     5461 aaattctaga aaagagaaag ctcgaaaaag ctttggaagt tgaaatggac gagggaaatc
     5521 tcggcgcaga tatgaactag atctaagtga gtagctgact actcgcttag gcggttgaca
     5581 gagatttcat aattttgcta tgctagtgag cacagttata caaaataccg gagtgctgcc
     5641 ggttaaaata gaggagaaag gcagatgcct acaattaatc aattaattag aatgggacgt
     5701 aaaacagtca aaatgggaac caagtctccc gcacttgaat cctgtcctca aaaacgcggt
     5761 gtttgtaccc gtgttatgac tgttacgcca aaaaaaccga actcagcgct tcgtaaagtt
     5821 gcccgtgttc gcttaagtaa tggaattgaa gttactgcat atatacctgg tattggacat
     5881 aacttgcagg aacactcagt tgttttaatt cgaggaggtc gtgtaaggga cctcctggtg
     5941 ttcgctatca tattattcga ggtgcaaaag ataccctcgg cgtaacagat agaaaacaag
     6001 gtcgctcacg atacggtgcg aaaaaaccaa aggcataagg agaataggaa atgggaagaa
     6061 aaaataaaac aataaaccgt ccaattctgc cagatgctaa atacaatagc cctgttatta
     6121 caaagtttgt aacaagaatg atgctagacg gaaaaaaagc aaccaatgtc gcaattattt
     6181 atgaagcgat ggataaatta aaagaaaaaa cagacaaaga tcctttagaa gtttttctta
     6241 aggctcttga taatgtaaaa ccgatggtag aagtaaaatc tcgccgtgtc ggtggtgcaa
     6301 cttatcaagt acctattgaa gttcgcgaaa gcagacgaga agccttggct atgcgctgga
     6361 ttattacagc tgctcgtgca aaaagcggac gaggtatggg cgagcgtctt gcaaatgagc
     6421 tcttagatgc atacaacaac acaggtactg catataagaa aaaagatgac acccataaaa
     6481 tggcagaggc aaacaaagct ttcgcacatt ttagatggta aacgagatgt agttgtcttt
     6541 ttctgacaac tacagttatt tttttacttt ttttacgcta tttttgaatt atctaaggca
     6601 tgagtcttgt tctttttttc taaatagtgt aaagtttgtc ttatccttaa aattttaagg
     6661 agtaattttt ctttcaaaag ggaaaaatta tctgggttct ttgagattca aaccgctttt
     6721 ataaaaataa aagtgtggca aaaccgtaaa gttgtttttg tatacaaaaa cttcaggctt
     6781 tgcgacccgt aaacacaaat atcatcgttg atggtattaa atatggtttg ttttcagggt
     6841 ttttgaatgg cttttttgcc aggctctcgg atgccaaacc atatcatcgg attgaacatt
     6901 tggtggtttt aggtggtacc agccaaaaca agttgcattt atagcttgtt ttacgcagtt
     6961 acaaaaagta cagtttcttg acttgcatct aaatcaaaac aatcgtttca atctcaaaca
     7021 ttggtgtgta gtgaatgtcg ggttcggtat gagcttgtgc attcaaaaag tatacaacac
     7081 tatatttttt actgccttta atctggtaag gaggatcaaa tggcaaagga aaagttcgtt
     7141 agaacaaaac cgcacatgaa cgttggtacc attggacacg tagaccacgg taaaaccacc
     7201 ctttcagcgg caatcacaaa gcatagcgcc caaaaatatg gcgacaaggc tcttaattac
     7261 gacgaaatcg ataatgctcc ggaagaaaaa gcgcgaggta ttactatcaa tacacgtcac
     7321 ctagagtatc aatcagacaa gagacactat gcacacattg actgtcctgg acacgctgac
     7381 tatgttaaaa acatgatcac cggtgctgct caaatggacg gagctattct tgtagtttct
     7441 gctcctgact cagttatgcc tcaaacaaaa gaacacattc tacttgctcg acaggttggt
     7501 gttcctggca tgatcgtttt cttaaacaag gttgatcttt tggacgatcc agatcttctt
     7561 gagcttgttg aagaagaagt tcgagacgtt cttgagcaat accaattccc aagagataca
     7621 ccgattatca agggttcagc ttttaaagcg ctcgctgacg gtgcttctgc tgaagacaca
     7681 gctccaattg atgaattact cgacacaatg gactcatatt ttgcagatcc agttcgagca
     7741 gtagatcttc catttttgat gcctgttgaa gacgttttta gtatccctgg tcgtggaacc
     7801 gttgttactg gacgaattga gcgtggcgtt atcaatatga acgaagaagt tgaaattgtt
     7861 ggtattcgac caagccaaaa aacagttatt accggtattg aaatgtttaa caaactactc
     7921 gataagggtg aaactggcga taacgttggt cttttacttc gcggtattga aaaaactgct
     7981 gtagaaagag gtcaagttct tgcaaaaact ggttcaatca aaccacatac aaaatttgaa
     8041 ggtcaagtat acgtactttc taaagaagaa ggtggacgac acagcccatt cttctcaggc
     8101 tatcgaccac aattttactt ccgaacaact gatattacag gaactgtata tcttccagaa
     8161 ggtgtagata tgattaaacc tggtgacaat acaaaaatca tcggtgaatt aatcaaccca
     8221 atagctatgg ataagggttt aaagttcgct gttcgtgaag gtggacgtac tattgcttct
     8281 ggtcaagtaa cagaaatttt agcataatgt atatttaaaa gagcagggga ctaactcctg
     8341 ctcttgtttg tggaggaaac atggctgccg aaaagattcg tgtcaggctt cgcgcttttg
     8401 atgtggactt gatcgatcaa agtgcaaagt ccatcgtgca aactgttcaa aaagcgggtg
     8461 caaaggttgc aggacctata ccgcttccga ccagaataaa taaggtttct gttttgagat
     8521 ctcctcacgt aaataaaaaa tcacgtgagc aatttgaaat gagaacacat aaacgcctta
     8581 ttgatattat cgagccatca tcagatgtaa ttgattcttt gatgaagctt gaattgcccg
     8641 ctggcgttga tgtagaaatt aagcagtaaa actgcaaaat aaaggtacgg tccctcggat
     8701 caggggatgg cggccaaagg taaggagata tcagaatgaa aggtctgatt gcaaaaaaag
     8761 tgggaatgac tcaggttttt gacgaaagcg gtaacttaac accagttacc gtgatccgcg
     8821 tagagcctaa tacagttgtt gcacttaaag acaaggaaac atttggctac gatgctgttg
     8881 ttcttggctt ggacgattta cgaaaaagcc gaattacaaa gccctacgcc ggtcaatttc
     8941 ctgaaggact tgcgccaaaa caaaagatta aagaattccg tgactttgaa gccgaggtaa
     9001 aagtaggtga cgaagttggt cttgaactgt ttgaaggaat tcgttttgtt gatgtaacag
     9061 ggatttcaaa aggaaaaggt tttcaaggtg ttatgaagcg ctggaatttt catggtggtc
     9121 gtgcatcaca tggttctaag tttcatcgcg aagctggtgg tacaggtcaa tgtacttctc
     9181 cagggagaac ttttaaaaac acaaaacaag ctggacgaat gggtcgagaa caagttactg
     9241 ttcaaaactt acgcatcgta aagatagatc cagagcttaa agttgtgatg gttcgcgggg
     9301 ctgttcccgg gaatagagac actacgctga tcatcaaatc tgcggtaaag aaagaattct
     9361 aggctacgag gaagatataa atggaaaaaa aagtctattc aaccgatggc aaagaactgc
     9421 gagcaattac ccttgatgat aaggtatttg gtctcccaat aaatgaagat gttatttatt
     9481 atgccatcaa taacgaatta gctaacaaac gtgttggaac cgcttgtaca aagggccgtg
     9541 ctgaagttaa cggaagcaat gcgaaaccct ttaagcaaaa aggaaccggt ggtagagcac
     9601 gccgtggaga taaaaaatct cctcttttac gaggcggcgg tgttatattt ggtcctaaac
     9661 cacgtgattt tagctttgtt ttacccaaaa aggtaaaacg attagcaatg aaatctatct
     9721 tgagtttaaa agctcaaaca gatagattaa caatcatcga agacttcact atagaaagtg
     9781 gaaagacaaa agatttagtt tcgattttaa aaaactttgt aaaagatgaa catagaactg
     9841 ttgttgtatt aaaagataac gatgctatgc ttaaaagagc gggaaaaaac attcctactg
     9901 tatctttttt atcttataat cgattacgag cacacgatct tttttacgga caaaaaatta
     9961 tcatgctaga gagcgcagta aaaaatcttt ctgattttta tgttgaagaa caggaggcaa
    10021 aataatgatg ctagaagata ttctcgttga acctgttttg tcagaaaaag caactttatt
    10081 gcgtgaagaa gggaagtatg ttttcaaagt acatcctaag gcaaataagt atgaaattaa
    10141 agatgcggtg caaaaacttt tcaatgtaaa agttgtagat tgcactgtca tgaatgtgtt
    10201 tggaaagatg aaacgagttc gttatagaac cggtcgtact tccagttgga aaaaagcaat
    10261 cgtcaaactt gctcctggcg aaacaattaa ggtatttgag ggcgtttagc tcttgataca
    10321 tatataaggg gaacctaaat ggccttaaaa gtatataaac ctataactgc tggcactcga
    10381 gcacgaattg atttagtacg cgacgaaatt accacaaata gacctgaaaa aagtttaaca
    10441 actggtaaga agcgaaacgc tggacgtgat gcaaatggtc gtatctcagt tcgtcataag
    10501 ggtggcggtc ataaaagaag gtatagagac atagatttta aaagagataa gcatggagta
    10561 ccaggaacag ttcgaactat cgaatacgat ccaaaccgaa gtgcaaatat cgctttgatt
    10621 ttctatgctg atggcgaaaa aaggtatatc attgctccaa aaggattaga agttggtcaa
    10681 aaaattcttt ctggcgaaag tgcaagccca actgtgggta acgcacttcc tttagaagca
    10741 atacctgttg gatttaccgt tcacaatatt gaattgacaa ttggtaaagg tgggcagttg
    10801 gcacgatctg ctggaacaag cgctcttgta gctgctaaag aaggcgacta tgttactatc
    10861 cgacttcctt caagtgaagt tcgtttagta cataaaaaat gctatgcaac catcggaacc
    10921 gttggaaatg aagatagaat gaatacccaa ttgggaaaag ctggacgaaa tagatggctt
    10981 ggtgtgagac caggcgttcg cggtatgtct atgaacccag tcgatcaccc gcttggtggt
    11041 ggtgaaggtc gaggtaaggg aagaaaccct gtaacaccat ggggacaacc atgtaagggc
    11101 ttcaagactc gcaaaaaacg caaggtatcg agcaatttta ttgtttcgcg ccgtaagaag
    11161 aagagatagg agataaccgt gtcaagatcc gttaaaaaag gaccttttat tgaaaagagt
    11221 ctttacaaaa aggttattga aatgaacaaa gcagcagaca ggaagatgat aaaaacatat
    11281 tcccgatgtt ctactattat tcctgaaatg gttggaaata cgatttctgt ttataatggt
    11341 aaatcttggg tgcctgtgta tatcacagaa aaccttgttg ggcataagct cggtgagttt
    11401 tctcctacac gtgttttccg cggtcatgct ggctcagata acaaagctgg gaggagatag
    11461 aaaatggctg aaaaaaaaga ctatatagcc aagacaaagt ttctcatcgc ctctcctaca
    11521 aaggttcgcc ctgtagccaa tgttgttaaa agaaaaaact gttcagaagc tatggcaatt
    11581 ttagaaaata tgccacaaaa gggtgcgact ttaattaaaa gcactatgaa atcagctatt
    11641 gcaaacgcat tatatgcaaa caagaagctt gatgaagaaa tgttgtatgt taaggacgtt
    11701 caaatagacg aaggtcctcg actaaaaaga atttggtacc gagcacgtgg tagagcagat
    11761 atgcttaaaa gacgtatgtg tcatattaca gtggtggtag cagaaaaggc gggtaattaa
    11821 gatgggacaa aaagtacatc caataggact acgtcttggc attaataaaa cctggcaatc
    11881 acgctggtat gctgacccaa gggaatacgc agatttatta cacgaagatt tgaagattcg
    11941 aaaaatgatt tctacaatgc ctgagtgtaa aaatgctgat attgctgaag tggaaataat
    12001 tcgacatccg caaagggtaa caattgttat tcatacagct cgaccaggtg taatcattgg
    12061 tgtaaaaggc gcaaatatcg aaaaaattgg tgctgaaatc caaaaaaaga ttgccaaaaa
    12121 agtgcaaatc aagataaaag aaattaaaag atcagagatt aatgcttctt tgattgcaca
    12181 aaatatcgct cgacagcttg taggtagagt ttcttttaga aaggttctaa aacaatctat
    12241 agcaaatgct atgagaggcg gagcccaagg cgttaaagta cgtttaagcg gtcgattagg
    12301 cggagcagaa atgtcccgca ctgaggagca caaagacggg cgagtaccgc tccatacact
    12361 tcgtgctaat attgattttg gttttgcgga agctcatact acctttggta aaattggtgt
    12421 aaaggtatgg gtttataacg gaatgatgta cggctacgaa caaaacgaag atgcaggtca
    12481 agtgcaacga aagcgacgtg aacgtacaga tcgagcagaa ggtgctgaca gagattctag
    12541 accagctcga ccaagccgta gtgcaaaagc cccaaggagt tagtcaatgg cacttagtcc
    12601 taaaagagta aagcatcgca aggtgcaacg cggaagaatt aaggggaatg caacgcgaaa
    12661 ttgtaatgtc gactttggag atatagcgct tgtcgcttta gaaccatttt ggcttacagc
    12721 tcagcaaatt gaagctgctc gtgttgcttt gaaccgaaga attaatcgta agggacaagt
    12781 ttggattcgt gtgtttccag ataaacccta ttcgaaaaag cctgctgaaa caagacaagg
    12841 taaaggaaaa ggtgctccag atcactggac agctgttgta aaacctggtc aagttttgtt
    12901 tgaaatcggt ggtgtagaat tggatacagc cgctcaagca attattttag ctggcagtaa
    12961 gttacctttc aaaacaaagg ttgcttacaa acaagacgta caataaggag aagcagaatg
    13021 gcaaaaaaag aaaaagattt gacttattct gaacttattg ttaagcggaa tgagctaaaa
    13081 aagaagtaca tggatttacg atttcaaatg gtaatcggcc atgttgataa ccctgtgcaa
    13141 aagcgtacca tgaggcgaga tattgccaca ttgaatacct taattcggca aaaagaaatt
    13201 gccggtttgg ataagtagga gttgacccgt ggaagaacaa gttgttcaag gtaaaagctc
    13261 aaagcgctct tttgtaggtg ttgtaacaag cgacaaaatg gataaaacca tcgttgttca
    13321 aatttctaca aaaaagctac acaagcttta taaaaaatac gttacgagtg tcaaaaaata
    13381 caaggctcac gatgaaaaaa atgatgcacg cattggcgac agagtgaaaa tcgttgagtc
    13441 aagacctcta agcaaagata aatgctggag acttgttgaa gtgcttgagc gagcaaaata
    13501 aggagtaggc tatgattcaa atgcaatcgt acatgaacgt tgccgacaac tctggagcta
    13561 aggttgttca atgcattaaa gttatcggcg gttcaaaacg tcgatatgca agtgttggtg
    13621 atgtagttgt tgtagcagtt aaggaaaccc ttcctacttc tacaattaaa aaaggcacta
    13681 tcgaaaaagc agttatagtg cgtatttcta aggaataccg aagatctgat ggaacttata
    13741 ttcgttttga tgataacgct tgtgttatta tcgatgcaaa taaaaaccca aaaggtaaac
    13801 gtgtatttgg tccagtagcc agagagcttc gcgacatgga ttacatgaaa attgtatccc
    13861 ttgcgccaga ggttctataa ggttcttaag gagaattgaa gaatggataa gaagttcaag
    13921 atccgaaagg acgacacagt agaaattatt gctggaaaag acaagggaaa acgaggtcct
    13981 atcgtaaaaa ttttacgaag taaagatcgc gttatcattt ctggtgcaaa ccttgtaaaa
    14041 aaagcaatga aaaaaagaag tcagcaggac aaaggcggca tagttgaagt tgaagctcca
    14101 attcatgttt caaacgtagc tattgtttgc aaaaaatgcg gtcctactag aattggattt
    14161 aaactcgacg gcgaaaagaa aacaagagta tgccgtaaat gtggagacac actataatga
    14221 gtaattatgt accccggctt aagaaagtct ataaagatac aatcgcccct gagcttttta
    14281 aagagctcgg atgcacttct gtaatgcaag tgcccgctgt caaaaaagtg gtagtgagca
    14341 tgggcgtggg tgaagctctc gcaaacaaga aactccttga cgccgcagta actgacctaa
    14401 caacaattac aggtcagaag gctgtgaaaa ccaaggcgaa aaaaagtatt gcgaacttca
    14461 aacttagaga aggtaatgaa gttggtgtaa tggtaacatt gcgaggaact caaatgtatg
    14521 agttcttgga tcgcttaata aacgttgcct tacctcgtgt aaaggatttt cgtggcatca
    14581 aggcgactgg ctttgatgga cgaggcaatt tttctcttgg tgtaaccgag caaattattt
    14641 tcccagaaat tgatttcgat aaaattgaaa aaatttcagg tatgaatatt aacatcgtga
    14701 ccagtgcgca aactgatagc gatgcacgtg ctcttctatc caagtttgga atgccgttta
    14761 ggaaataggt aggagatcat ggcaaaaaaa tcattgatta ttaaggcaag ccgcccacaa
    14821 aaatatagta ctcgcaagta caatcgttgt ttagtgtgtg gtcgaccacg tggatattta
    14881 cgaaagtaca aaatgtgcag agtatgtttt cgtaagttag caagcgaagg cctgattcca
    14941 ggcgttacaa aatcaagctg gtaggaggga gtagataatg agtgcttcag accctattgc
    15001 agatatgctt actaaaatca ggaatgcagc tttggctcgc cacgaaaaag tagatgtttc
    15061 tgcttctaag attaaattag aaattgttaa aattttaaaa acagaaggtt atattaaaaa
    15121 ctttaagaaa attagccaag atggtatgaa ttctattcgt atttttctta aatatgatga
    15181 taaaaacgca tctgttatca aaggtatcca aaagatatct acaccaggta gacgagtcta
    15241 ttctgggtat aaggatctgc cccgtgtcta taacggttac gggactgtta ttttatcaac
    15301 ctctttgggt gtaacaactg gtcggaaggc aaacgaaaag atggttggcg gtgaacttat
    15361 ttgcaccgtt tggtaaaagg gaaagaaaat gtcaagaatt ggtaaacttc ccgttcaaat
    15421 accagcgggt gtaaaagttt ctgtaagtgc aaacgaaatt tctgtagaag gaccaaaagg
    15481 aaaactcagt caagatttta acaatttagt ttctattaag gttgagggta ccgatgttgt
    15541 ggtaaatcga gcaaatgact caaaaccagc gcgttcagca catggtttat acagaagcct
    15601 tgttgaaaat atgattcaag gtgttaccga aggctttagc aaaacattag ttattaacgg
    15661 tgttggtttt agagctgaag tgcaaggtaa tctcttgatg ttaaaccttg gttattcaaa
    15721 cgacatttat gcggggattc cagaaaatct taccgtagtt gttgaagctc aagggaaatt
    15781 aacaatcagc ggcatagata aacaagccgt tggtgagtat gcagcacaaa ttcgtaagtt
    15841 gcgacttcca gagccatata agggcaaggg aattcgttat agcgacgaag tcattcgaag
    15901 aaaagtcggt aaatctggcg tataatgagg taaagcgatg cataaaaaac gtaatgaaaa
    15961 agatagaaaa cgtcttaaga gaaagattca cattcgtaag cgattgcacg gtacagctga
    16021 ccgcccacga atgactgttt ttagaagcaa taaaaacttg tttgtgcaag ttattgatga
    16081 cgaacaaggc aaaacacttg cttctatctc taccttagaa aaagagtttt catctatgaa
    16141 ggcaactgtt aaagatgctg agaaactcgg cgaagcaatg ggcaaacgac ttgttgaaaa
    16201 taaaatcaac acagttgttt ttgatcgaaa tggctacctc taccatggtg tagtgaagtc
    16261 tttagctgat ggtgcccgca aagccggaat cgtgttctag gagtagatat gtataaccaa
    16321 agaaattatg ataaagacca gccaaaagaa tttgtagaaa aattggtaaa gctgaaccga
    16381 actgccaagg tagtaaaagg tggaaggcga ttttcttttt ctgcattaac cgtggttgga
    16441 gataaaaaag gtagagttgg ttttggattt ggaaaagcca acgatgtcag tgaggcgatc
    16501 cgaaagagca ttgaaaaagc aaagaaaaat atggtagtac ttccacttaa aaatggaaca
    16561 ataccacata aaattcaagc aaaatacaag ggttcttctg tcttattgct tcctgcttgt
    16621 tctggtaccg gaattatcgc tggtggacca gtgcgggcgg tattggaagc agctggcgca
    16681 acggacgtta tgtctaagtc acttggctca aacacatcca taaatgtagt acaagcaaca
    16741 tttgctgcta ttgatggctt aatggatgct cgagttattg ctaaaaacag aggaaaagct
    16801 ctcaaggatg tttgggggta actatggcta agaataaaag aattaaaatt actttagtac
    16861 gaagcactat tgggcaaaaa gcgccaaagg ttgctactgt tcgaagtctc ggtcttaaaa
    16921 aactaaattc cacggtggaa catgaggtaa atcctgctat tatgggtatg gttgcagctg
    16981 tttctcactt ggtaacatac gaggagttga attaatgtct gattttactc ttaaagcacc
    17041 agttggtgca aataaagcaa aaaagatagt tggtcgaggt tcaggatctg ggcttggaac
    17101 aactgcaggc cgcggaaata agggtcaaca atcaagatct ggtggtggta cctatgtagg
    17161 ttttgaaggt ggacaaatgc ccttataccg acgcattgct attagaggtt tttctaacta
    17221 tccgtttaaa aaagaatatg ctctttttaa tcttcgtgat ttggaagcaa aatacgaaaa
    17281 tggtgaaaca gtttctaagg aaacactcat taaaaaaggc ttgttaaaaa aagcgggtat
    17341 tttagtaaaa gtattgggtg atggtgagtt gacaaaaaaa cttactgtta atgttgacaa
    17401 ggtttcagct tctgcaaaag aaaaaataga aaaaattggc ggaacagttg cgataagtaa
    17461 cgaataaaag tgagaaaaag atggcaaaca atcctatagt taatatgttt agagtaaaag
    17521 aactacgaga gagaatcctc ttcactttta ttgttcttgc tgtgtatcga tttggtggtg
    17581 ttttaacaat accaggcata agtgcacaag ctttaacaac gtacttttct tctttaacaa
    17641 aggggaatgc ctttgttgat tatatgaact tttttgcagg tggagctttc tctaagtttt
    17701 cactttttat gcttggtgtt accccatata tttcaacaca gattattatg caacttgcat
    17761 tgataatttt cccttcgctc aagaaaatgg tacaagaaga tggtggacaa aagaaatttc
    17821 aaacatggac acgaattgga actgtttttg tcagcttaat ccaatctctt gctgttgtag
    17881 cctacgcata ctctattcca aatgctgttt taatacaaaa tatttggctt tttagagcaa
    17941 ttgcgatggt ttcaataaca acaggaacga tggctactat atggcttggt gaacaaatta
    18001 cttctagagg catagggaac ggtgtttcga tgcttatttt tgcaggtatt gtttctcgtt
    18061 taccatctgc tgtttgggaa ctgggtggtt tagttaaaaa caatgaaatt aacgttgttt
    18121 ttgtcgtagt tgtcttactt atgtttgttg ccgttgtcgc tcttgtcgtg tatgaacaac
    18181 aaggtcaaag aaagatacct gtgcattatg cgaaacgagt tgttgggcgt aaaatgtatg
    18241 gtgggcaaaa tacttacatt ccatttaagc ttaatccatc aggtgttatc cctgttattt
    18301 ttgcctcatc ttttttaacc tttcctttac aattagctac aagctttggt gttaaatata
    18361 gatggcttgc aagacttgca aactttttaa ctccttcagg ctggtggtat aatattatac
    18421 aagttttgtt aatcgtgttt tttgcttact tctacactca agttaccttg aaccccacag
    18481 aaattgcaaa aaatatccgg gaaaatggcg gctcaattcc tggaattcga actgacaaaa
    18541 tggaagaata tatgcaaaaa gtattgaaca ggctcatgtt acctgggtcc ctgtaccttg
    18601 cttttattgc agtaattccg actattatcc aaaatctatt tggttttcca cattcgattt
    18661 cttctataat cggtggaact tccttattga ttttagttgg agttgattta gatacaatga
    18721 gtcaagtgga agctttatta aaaatgcatc atcacgacgg cttggtaaaa aaaggaaagc
    18781 tacggtctcg taacctttag cttgagcagg ttttttagaa cttctaaaaa acctccttgt
    18841 tttacgtatc tatggcatag aaagctttgt tttttatgcc ttcggtgttt tctttatgaa
    18901 aatatcaaaa aaagctgaaa attttttttc tagcttgtag aagttttttt ggaaatagtg
    18961 tttaatactt aaatgttgaa ttgtaatcat tgcgatttgc tgagattcaa caatgacact
    19021 atgaatttat ccagggggat attatgaaag ttagaactag tgtaaagccc atttgtgata
    19081 agtgtaaggt aattaaacga tttggcgttg tccgaattat ttgtactaac ccgaaacata
    19141 agcaaaaaca gggctaagcg gagcttaagg agaattagat ggctcgaatt gcgggagttg
    19201 acctcccaaa taagcatgtc aatatcgctt tgacgtatat ttacggaatt ggacgatcca
    19261 gtgcagatga aatctgcgaa aaaacaaaga ttgatccgaa tcgtatgatg aacgatcttt
    19321 cacaggatga agttggtgtt attcggacac ttattgatgc tgaatataag gtagaaggta
    19381 gactacgatc tgaaacaggt ctaaatatta aacgccttat ggacattggc tgctaccgtg
    19441 gtcttcgaca tagaaaggga ctacctgttc gcggtcaacg tactcgtact aattctcgca
    19501 cacgcaaggg tagaaaaaag accgttgcag gcaagaagaa ataggctata ggtggaggta
    19561 ttttaaatgg ctaccgttaa aagacgaaaa gagaaaaaaa gcgtatatga agggaatgta
    19621 tatattcaag caaccttcaa caataccatt gttaccatta cagaccttaa aggaaatgcg
    19681 ctctcctggg cttcatctgg tggtttaggt ttccgtggag caaaaaaatc aactcctttt
    19741 gcagcacaaa ctgttgcaga aacagctgta caaaaggctg tgagttatgg cttgcgtgaa
    19801 gtgcatgtgt atgtgaaagg ccccggtgtt ggtcgtgaat ctgcggttcg atctttaggc
    19861 gctttaggtc taaaggttaa atcaatttcc gatgttacac cgatcccaca caatggatgt
    19921 cgacctagaa aaacacgacg aatgtagtga aggagaacta agaaatggga agatataatg
    19981 gtcctgtttg cagactttgt agaacagaac aaagaaaatt atacctaaaa ggcgatcgat
    20041 gcaatagcga taaatgccct ttaaataaaa aaagaggagc tcctggtaag gatccgaagg
    20101 ctcgaattgg gaaaagaagt gaatacggac ttcagcttcg agaaaaacaa aaacttaaga
    20161 gaatgtacgg aatgtttgaa aaacaattcc atcttacatt tgaaaaagcg aatagaatga
    20221 gcggtgttac cggtgaaaat cttattatct tgctcgaatc acgcctcgac aactttgtat
    20281 atcgcatgca cttggctgca agccgatcac aagctcgaca attggtttca catggacata
    20341 ttcttgtgaa cggaaaacca gtaaatattc cgagctatca cttaaaacca agtgatgtcg
    20401 tagaagtaaa agaaaaaagc aagaaactgg tgagcatttt agattcttta aaagaagttt
    20461 ctaaatcagg cgtttcttct tggatagatc ttgatattga tgcacaaaag gcaacttttg
    20521 tctcatatcc atctcgtgaa gaggtagcag atcttgccga cattaaagaa cagctcatcg
    20581 ttgagcttta ttcaaaataa ggagtattaa tggcacgcaa aaatctgctt aaaggattta
    20641 agaaaccaaa gggcataaac tttgaacata ttgatgtaaa caatcattat ggtaagtttt
    20701 cagcctttcc ttttgaaact ggttttggta cgactgtagg caatacattg cgccgagtat
    20761 tactttcttc aattcaaggc tatgccgtta gttctgtgag aattacgtca tttgatgcag
    20821 atgctgttcc tcatgttatt tctagtgagt ttgaggctat tccaaatgtt gttgaagata
    20881 cgctagaaat tttaaacaca cttaaacaag tgcgacttcg attacctgaa gatattgaac
    20941 aagatacttt tacctttgag tttaaaggac ctggaaaagt aacgagtgat atgcttgaaa
    21001 aagggggcag tctagaagtt tttacaaaag atatgcactt gtttactatg atggaaaacg
    21061 cgcatattga atttgtagta caagttgacc ttggccgtgg atatgttcca gctgagataa
    21121 atgaaaacta catcgagttt gtaaacacta tcccaatgga cgctattttc actccagtta
    21181 ctcgtgttca atactcaata gaaccttgcc gtgtaggaca acgaaatgat tatgataaat
    21241 taattattga aatttggaca gacggtacta tttcacctga ggatgcttta gctgaaggtg
    21301 cgaagattgc aaaggatcat tttgcgatct ttataaactt taatgaagat gatgatcata
    21361 agcaagacga tcaagatgaa gcagatgaaa gaattcgtaa aattctcaat acacctgtgg
    21421 aagaacttga gttgacggta cgttcatcga actgtttgaa aaatgctaac attcgcacta
    21481 ttggtgaatt aacaaaaaaa tcagaagagg atattgctaa gactagaaat ttcggcaaaa
    21541 agagtttagc tgaaattaaa gaaaaactac aagaatggaa tttaagttta ggaatgactg
    21601 actatagcca tttacgtgca aaagttgact tagcagcctc gaaggaagaa tcagatgaat
    21661 cataaaatgg gttttaatgc actttctcga acaagtgcac atagacgcgc tatgactcgc
    21721 aatatggtca cctcgttgtt tcgatttgaa agaataacaa caacaaaggc aaaggcgctt
    21781 gaggttagaa aaaccgctga aaaattgatt acacgaagta aagttgactc tgtacacaac
    21841 agaagacaag ctgctcgttt tattcaagat aaaataattc ttgcaaagct ttttactgat
    21901 attggtccgc gcatgaaaga tcgagctggt ggctacacaa gagttctcaa gctcggtttt
    21961 agacaaggcg atgctgcaga tatggtaatc ttagaattag ttgatttttc tttagatggt
    22021 gatacaaagc cagcttcaaa gtcagaaaaa aaagatgtga aaaaggccaa tgctgacacg
    22081 aaggcaaagg tagatacaaa gtcaaaaacg aaggcgaaaa ctactgaaaa agctgaagat
    22141 ttaagcaaag caacagctgc tgagccatct actgaaaacg aaaagactga agaataggag
    22201 atatctatgt caaaatcatt tagaggaaga aatcttagag aattacccgc aagtggtaga
    22261 ggtctttgtg cgcaatgtaa acgtgaaggc gttaaaattt tacatgaagt tgagattgac
    22321 ggcgcaaaaa acaagatttg caaaatttgt aaggctacaa acaaaaacaa agctaataaa
    22381 gcaagctaat taaattttgt aaaaaactgc ctgcgggcag ttttttttgt ttaaagtact
    22441 ttagatatga ttttttgcta taaatcttgt atatttatag acaagttaaa aaaaaaaatg
    22501 taagatatta caaaatggag aagaatgaat tgttgcgtat aaattgcgta atagttcatc
    22561 aaaaaaagag gaggagctat gaaaaaagta attaaaacaa tactaatagc tttatttaca
    22621 ctcatgtttt ttattgggtg ttcaaaagaa gatacttctg ttattaaaat cggtggtatt
    22681 tttcccttat ctggagatgt agctgtttat ggcgttgaag ccaaaaacgg tatagaactt
    22741 gctatattgg aaatcaatgc gaagggcgga gtaaatggca agcaacttgt tcttgtttct
    22801 gaagatgatg aaggttcacc tgaaaagaca gtaaatgctt ttaaaaagct tacttcaaaa
    22861 gataaggtta aaatcgttat tgggtcttta acttctggtt gtactgcagc aattacaaca
    22921 cttgcgcaaa atcaaaagat tcttttaatt gcaccagctg caactatgga atcaattacc
    22981 gatgcaggta attatgtgtt tagggcatgt tttattgacc cattccaagg aaccgttggt
    23041 ggcaaatttg cggctgaaat cctggactca aaacgtgctg ctgttttgta tgatacagac
    23101 aatgattatt ctgtcggttt gtatgaaaac tttaaaaaat ctttcgtggc aaatggtggt
    23161 tcaatggcag cggaagaatc ctatacttca ggtgatgtag attttaatgc tcaaattaca
    23221 aaaattaaat ctgcaaatcc tgatgttgtt taccttcccg actactatgc cactgtatct
    23281 ttaatagcga aacaattgcg cgcacaagat attcatacac caattgtagg tgctgatgga
    23341 tggggaggac ttgtagataa tgcaggcgat gaagttttaa acggatttta ttctgatcac
    23401 tatgcggctg actcaacaga tgatatcgtt gtgtcttttg taaacgccta taaagatagg
    23461 tataagtcaa ctcctgtttc ttttgcagcc ttaggttacg actgtgtgta tatcttaaaa
    23521 gatgcaatgg aaagagctgg atctgaagag gcaagtattg ttcgaaatga ggttgaaaaa
    23581 acaaatggag cctatgttac tggacaatta acctttgatg caaaacgaaa cccgattaag
    23641 tctgctgtaa tgatggaaat tgttaaacaa gattctgtct tgactcctgt atataaggca
    23701 actgttaatc cttaagcgtg gattttaaac taaaaaaggc ctttattgca aaaatgtgat
    23761 ataggctttt tttttgctat ttacctattg gctagaaaag ggtaaaataa attcaaactt
    23821 tcttattaca ttgcgagagc gagtttttat ggaggagtaa tgaaaagtat ggctaaaata
    23881 gtgccatcct tttcatttgc acgtttgcta gcaaacgtgg gatttactgc actttctcat
    23941 tacattgcga aagtgctgaa aaaagtcaat ttcgaaaact gaaattttcg aaattgactt
    24001 tttatggagg agtaatgaaa agtatggcta aaatatcgcc atccttttca tttgcacgat
    24061 ggaggtaatg tggacacaat ttcgatggta atacaacaac tcataaatgg gatttctctc
    24121 ggtagtattt atgccctcat tgccttgggc tataccatgg tatacggcat cgtaaagttg
    24181 ataaattttg cacacggcga catactcatg gttggtgcct atgttggttt ttttgtgcta
    24241 tctgcttttg gaacaagtcc gtttggcttt tttgtagcaa taattatttc tatggtagtc
    24301 tgtgccttgt ttgctattgt gatcgagcga tttgcctatc ggcctttgag aaatgcacca
    24361 aggttgaatt ctttgataac tgctattgcg atgtcattga ttatacaaaa tgctgctcgt
    24421 gttattccat tcattggacc gaatccgaga caatttccaa cgcttgcaaa atcgacagtg
    24481 tatattggtt ctttttcact ttcaaatatt cagttaatcg ttatagcttt gtctgcaatt
    24541 ttaatgttga tacttgctta tataattaaa tttacaaaga taggaaaggc tttgcgtgct
    24601 gtatcttttg ataataaggc tgcaagttta atggggattt ctgtaaataa gacgattagt
    24661 tttacctttg ctctcggtgc agctctggct gcagtgggag gagttttgta tgcaacagct
    24721 tacccgcaaa ttacaccaac tatgggtgcg actccgggct taaaggcttt tattgcagcg
    24781 gttttgggcg gtatcggctc tatacctggc gcgatgatcg gcggttatat cttgggttta
    24841 gcagagactt tcgcgaaggg ctttattagt tcacagtttg caaatgctat ttcctttgca
    24901 attttaatta ttattttact tgttaaacca actggcttaa tgggaaaaaa tgaaagggaa
    24961 aaggtataag aatgcaaaat ataaaaatgt tcaataaaaa aatgattttt cccctgattg
    25021 tttcggtttt tttgctcatt gttccgacta ttctaataaa acttgcaatt atcgatgcat
    25081 attttgcaca aattataaca ttggctgcta taaatgcaat tatggctatt tcagtaaata
    25141 ttatttgcgg gtttacaggc cagctttctc ttgggcaagc gagctttatg gcgattggtg
    25201 cttatatgag cattcttttg accgaaattc ttggcttgcc tatgggcttg agtattattc
    25261 ttgcgggatt ttctgctgcc tttgtaggtt ttttgattgg ttttccgact ttgaagctac
    25321 ggggtgatta t
//
LOCUS       DAOSSW010000017        23774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_2312_length_23774_cov_7.970024, whole genome shotgun sequence.
ACCESSION   DAOSSW010000017 DAOSSW010000000
VERSION     DAOSSW010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 23774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23774
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2312_length_23774_cov_7.970024"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PKW26_02820"
     CDS             complement(<1..606)
                     /locus_tag="PKW26_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOQ92533.1"
                     /translation="MLKALNNEENIERSFYKTEILLGPGTVQKLNTKTIAVFGLGGVG
                     SFVVEALARSGIGSFILIDNDVISPSNLNRQIYALHSTIGQAKVDLAKERILDINPHA
                     RVSTHKLFYLPKEKHESYQNSIDGEKKNLVKSPLASLEAQEKLEAELSLCDYIIDAVD
                     TISAKILIIERARELSIPHISSMGTGNKVDPSQFKIADIYKT"
     gene            complement(587..2377)
                     /locus_tag="PKW26_02825"
     CDS             complement(587..2377)
                     /locus_tag="PKW26_02825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOQ92534.1"
                     /translation="MADKTIPLWSLESIYPSIESKEHQEAKTNLKNGLSELASLVATK
                     PSREDFPSWLNTYLEKYNKTISLFQSMYAYAHAIYSCDTTNTAFLNNLSQIEQALVEV
                     QDIGFLFTKILTEHKQALPNFYTAYPQYTSYSFILNEYIEGDSHYMSREEENLANSLQ
                     RYASSAWSRLQEQIISSLVDAETGKTFNELRNEAYAQERNVRKTAFEKERALLKSSEI
                     AIAACLNNIKGATLELNKKRSWEEPIDKALFANRLSKKSLDALISAIEDSLPFWQDYL
                     KTKASLLGLEKCEFYDLFAPLVSENAKEKIWSFAEAQKYIVETFGQFSKSLADFAQKA
                     FENNWIDAKVRQGKVGGAYCTDFPYHKEPRVLSNFTGTFSDILTLAHELGHAYHFDCI
                     KDEDFSLSHYPMTLAETASIFAETVVMHDAIGRSQGFEKVKLIESSLSDSAQVLVDIL
                     SRFYFERSVFEHKKEGELSAQDFSRLMKEAQEKTYGSGLTDKKHDLMWAVKGHYYGPD
                     LDFYNFPYAFGLLFARSLFAVHKENTAKGDSSFPTTYIELLKNTGRFSCEEVCAQAGF
                     DIQTKAFWKKGIDSFAEELAEMKAYAQSIK"
     gene            complement(2396..4144)
                     /gene="pheT"
                     /locus_tag="PKW26_02830"
     CDS             complement(2396..4144)
                     /gene="pheT"
                     /locus_tag="PKW26_02830"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOQ92535.1"
                     /translation="MPKIEVNEALFFNLLGAKYDYDSLEEKLTCAKAELDEKPDPSLA
                     EAERSIKIELNDTNRPDLWSTAGIARQLRMHNGLAKSADYSSFLSTEKKNLPTNEGLK
                     NKTDRFIKVDPALKDIRPFMTAFLISGKPIDEHMLVDIIQTQEKLCWNYGRKRRSIAM
                     GVYRSQLIEWPVQYKACDPDKRRFIPLASESEMSLREINKEHPKGKDFGWIIENEKLF
                     PLIVDAKDEVLSMPPVINSAKLGAVEVGDSDLMVEMTGTDMDSLLLATNIVACDFFDA
                     GYTILPVRVEHPYETGYGKTITTPFYFQKTTQARLSAINTLLGHNLTKDEVQEALTKM
                     DCHVSIEEKEGESLFTLTPPAYRNDFLHEVDIIEDVAIGKKLSFFTPEKPHDFTIGRL
                     LPLTLFSRKAKSLMVGLGYQEMIFNYLGSKRDYIDRMNISADSVIEVANPMSENYQFV
                     RPSIIPSLLAAEAVSANAVYPHNIFEIGKVAFLCDEENTGTRTRQNLGFVSLSQDANF
                     NTGASEVAALLYFLGHEYKVKESDDPRFIKGRQALVFKGETEVGIFGEIHPQVLENWD
                     IGVPCFACELDLEELI"
     gene            4292..7519
                     /gene="carB"
                     /locus_tag="PKW26_02835"
     CDS             4292..7519
                     /gene="carB"
                     /locus_tag="PKW26_02835"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOQ92536.1"
                     /translation="MKRTDIEKILIIGSGPIVIGQACEFDYSGTQACKALRELGYKIV
                     LVNSNPATIMTDPVMADATYIEPLNVERIEQIIVKEKPDALLPNLGGQTGLNLAFELS
                     KKGILEKYGVKVIGVNLEAIERGEDRQIFKAEMIKIGIDTPRSDIAHTIEEAEQIAEN
                     LSFPVVVRPAYTMGGSGGGLVYNREELHTTVSRGLELSLTHQCLIEESILGWEELEVE
                     VVRDKKNQMIAVCFIENIDAVGVHTGDSFCTAPFLTIDKDLERRLKEQAFKIVENIGV
                     IGGTNVQFAYNRKEDRIAIIEINPRTSRSSALASKATGFPIALVSAKLASGMSLDELP
                     YWRDGSLEKYSPDGDYMVMKFARWAFEKFNGVSDSLGTQMKAVGEVMSIGKTFKETFQ
                     KAIRALENGRSGLGFAKDFHKKSVDELCDALKVANSERYFMLYEAIRKGASLTRLEEI
                     TFIKKYYLEQIQELVQLEEELLKNRGMLPSDRLLEKAKKDGFSDKYLAQILNIREKDI
                     RKKRKELGIVEGWHAVPVSGVEDKFYYYSTYNAPDTVSVTDNKKKIMILGGGPNRIGQ
                     GIEFDYCCVHASFALKDLGYETIMVNCNPETVSTDYDTSDRLYFEPVTTEDVLQIYEK
                     EKPEGVIVQFGGQTPLNIARELSDAGVRILGTSIDSIDIAEDRDLFKTMMETLNIPMP
                     EAGMASNYEEALAVAEKIGYPLMIRPSFVLGGRGMEVIYDQKMLKEYIERAVGVSPDR
                     PLLLDRFLKDALECEADALSDSENVFVPSVMEHIELAGVHSGDSACVIPPVSITKEHI
                     KTIKTYTQKIAQSLKVCGLMNMQYAIEGDKVYVLEANPRASRTVPLVSKVCNVQMAKL
                     ATRLMMGESLASLELKEKKIPHFGAKEAVFPFDKFDNVDPVLGPEMRSTGEVLGMSEN
                     FGLAFYKSQEAASASLPDLPEEGKVKKVLISLSEKVHQAKDAVEVGRGFHKMGFSILA
                     TEGTAAFFQKEGIPCEVIAKLNEGRPNVLDVILNKEVCLAINTPSAKKDVIKDEAAIR
                     QAVIKYKVPYITTLAAAKAAVQGILASSKGKGSVKSIQEFHKEIR"
     gene            7580..8155
                     /locus_tag="PKW26_02840"
     CDS             7580..8155
                     /locus_tag="PKW26_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOQ92537.1"
                     /translation="MHLYQIFIIALGLSMDAFAAALCKGLAMSKKNLKIALLIAVFFG
                     GFQALMPLLGWLLAFSFATYIEAYDHWIAFGILTILGGKMLFEAIKNRNEGCPVYSNK
                     KEVYKRELFQLGIATSIDAFAVGLTFAFFEVAILPAIAIIGLVTFALSLLAVYLGNFF
                     GEKLKTKAEIFGGIVLIGIALKILIEHLFVA"
     gene            8253..10160
                     /locus_tag="PKW26_02845"
     CDS             8253..10160
                     /locus_tag="PKW26_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOQ92538.1"
                     /translation="MQSTMPKMLREVAKQHPDVPAQYFKDGTGRFQSVRYSDLYKTIL
                     DLASGLLHLGVKRGNHVGIISDDRKEWQEASMAIMGLGAADVPRGCDATKADLSYILS
                     FAECEFCFVENQAQVEKCISIREAIPNVKTLIVFDLVEKTALEAAKKAGFSLIQYNDV
                     IENGTKERKKNPGFVEKEIDKGNEEDIACIIFTSGTTGEPKGVMLCHRNFLTQLDELP
                     ELITLSPGDKALCVLPVWHSFQRLCEYMILFQAGSMCYSKPIGSILLADFAALNPQLL
                     PAVPRVFEAVYEGVLRTMRKGGGIKYLLFKFFVGIGILHSKIDRLLLNKTALFKKRNR
                     FFDWLLLVLPWLFLYPLKLLGSVLVFKKIREKLGNSFHVGVSGGGALPPIVDEFFWAV
                     GINIVEGYGLTETAPVISVRSMHNPTFGTVGYAIRGVEAKIVDEDGRELGPGQKGTVM
                     IKGGSVMKGYYKKPDLTSEVLSEDGWLDTGDLGLLTIDGQLVLRGRKKDTIVLRGGEN
                     VEPLPLEMRMMESRFIAQAVVLGQDERYLAAMIVPSRDDVESYAKEKMIDVSVFEKLL
                     EHDEIQKLFETEIKELVNAKSGFKIFERISRFVLLKKPFEPGVELSAKQEVMRYKLPE
                     LYPKEMKLLFK"
     gene            complement(10167..11312)
                     /locus_tag="PKW26_02850"
     CDS             complement(10167..11312)
                     /locus_tag="PKW26_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOQ92539.1"
                     /translation="MLKKTNLNTIFLFALFALFFVALVGIFYPFFYVILWASLLYILL
                     NPLYKKIQQKITQKKRFLEIKRHALAAIFSIATLILIIGPLLLLTIQLAQQLLAVFKD
                     IELFLRTNPTFFSDSSFIKKTIDILNDFGIENELFSQVDGLHIRAFILQTIQYYSNTL
                     LMISKNLLENTANFILSILFVVFALYFFYLDGAYLFTLLIKAIPINPKHMDTLAKKFS
                     ETIRGLLSGYFLVALYQGIIAFILMKIFGIKAALLFSVLLMFSSFIPLFGAGLIWFPL
                     GIILLFTDSVIKGILFLVLSAFFISFLDNFLRPFFLQDRIKVHPLLIFFAILGGLKIF
                     GLNGLILGPTVIILFFAVLDMLVSFDEENFVEENHNPREKNTIEPIE"
     gene            complement(11322..12539)
                     /locus_tag="PKW26_02855"
     CDS             complement(11322..12539)
                     /locus_tag="PKW26_02855"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HOQ92540.1"
                     /translation="MHTNKEENYTRESMFGRPPKDALSLLDNFDSILQEIKPLSSKHL
                     QSLPYNIQQLSLQLTSERSSRKKTYMNSRVEMISYTRYFMWWNLFRLTSLFARFPKDF
                     FQNLLQTEEETYCLDIGSGPLTLPIALYLARPELRDKKLNWYCLDPSSTSLSLGEDIF
                     LSIVAKLRGRAWNIIKVKGLFGETIRNKTKLICSANMFNEMYLEDRRPLEELVKLYAK
                     TLSQYTKDENASVLLVEPGIPRSARFISLFRDSLLRQNFSILSPCPHEEACPMPGTRD
                     KKWCHFTMNAENAPEKLQKLSRDAALAKEKIVLSYVFASKCPQKNKDDSALRISSLPI
                     RLPKQKIGHYACGKKGLMLFVKNKYTIAENAALFSGDLVQQNKEKAETKQGNLQKKRT
                     QIDSKSGAIIIDS"
     gene            complement(12539..12889)
                     /locus_tag="PKW26_02860"
     CDS             complement(12539..12889)
                     /locus_tag="PKW26_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOQ92541.1"
                     /translation="MRKEFSIGDIEELTQTKAHILRYWEEIIPSIAPKKNISGRRVYT
                     EQDLFLLLRLKHLVQDKKYTLEGARNKLIEEASDDLSLLDENGSPVHIRQSFNELRTD
                     LIELFLIVKKHRKP"
     gene            complement(12889..14340)
                     /gene="der"
                     /locus_tag="PKW26_02865"
     CDS             complement(12889..14340)
                     /gene="der"
                     /locus_tag="PKW26_02865"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOQ92542.1"
                     /translation="MKKKQNQKKQFSKSFTPKHQKTYDSPYSMDSDYKNRPRIVIAGR
                     PNVGKSTLFNRFLNRRLAIVDSKPGVTRDPVEKNAFILGYPVKLIDTGGFKLDRDHGS
                     DEAIMDELVVEKTLETLKNCDLIVLVLEVGEITAEDEEFIALLRPYWDKVIAAVNKTE
                     GGRKEEEAWNFMQFGFQSLHLISASHGDGITALAKEIISRLDFSAVEEGSEEKPIKVS
                     IMGKPNTGKSTLSNVLTKSSASIVSDYAGTTRDVVEGFFSYKGKNFHLLDTAGIRKKT
                     KVKEDIEYYSVNRAIKTLQNCDLVFYILDAEEGLTDQDKKIISLAHDRGRGIIFILNK
                     WDLQEDTKQSRKKAEEDIRIHFGHMIWAPVLCISALEKQGINPLLNTALKLYGQLTKK
                     IDTPSLNLALKEWIRSYPPPQSKASQFKLRYCVQSNTNPISFILFASRPEVVPQSYIT
                     YLKNKIRSDLGFDNIPIILELKASRQKWEDRES"
     gene            complement(14348..16033)
                     /gene="ptsP"
                     /locus_tag="PKW26_02870"
     CDS             complement(14348..16033)
                     /gene="ptsP"
                     /locus_tag="PKW26_02870"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOQ92543.1"
                     /translation="MNILSGISASKGLAIEKAHFIVQSKKKQVEKTRISQSEKEAEWK
                     KFQKALELTIKDFSLLLQTNNPDEKKLIETYLLMLNDQEFINQIKLNFDNSSYNVDFI
                     VDSVVNESASLLRHTNDEYLSQRADDILDVFGKVIDRILGNKIENIQDIDANSIVVSQ
                     NISGSFAISLFQKGIKGLLLSDGGLSSHTAILARSYGIPFIFGIHKLSEHISHGDTII
                     LDANEGSVCISPDLKTIEKYQKLKKEAEERENLLAQYINKKGKTKDKVEINLFANIGS
                     VEEADLALKHGAEGIGLFRTEFLFMDAERPSDLLDETKQYQVYSEVLKIMGEKSVVIR
                     TLDAGGDKVLSVTENSISKNGKKTKYIKPEIEENPLLGNRAIRFCLGQKDIFKTQLRA
                     LLRASIDGNLSIMLPLITHKEQVLETKALIDEIKKELTEKNIAFKKDVPLGIMIETPA
                     AAILAEDFAEIVDFFSIGTNDLTQYTLCVDRENTGVSALYQEMHPAVLALIEMTSKAA
                     KKHNIPISVCGEMAAREDEIKALLKRGIRSLSMRANKISEVKAFLETISLKSL"
     gene            complement(16077..17507)
                     /gene="mnmE"
                     /locus_tag="PKW26_02875"
     CDS             complement(16077..17507)
                     /gene="mnmE"
                     /locus_tag="PKW26_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOQ92544.1"
                     /translation="MLENKTYIPEQAIAAIATPLAPSALAVIRCTGKEVISLVAAIFS
                     RPEALQKAAGNTTLYGWIVDGEKKVDEVILAVYRAPKSFTGEDMVEIFCHGGISVVRS
                     IYALLLSKGFSEAERGEFSFRSFLNGKTDLTKAEAIREIIESKTQDSSSRAAGRLSGS
                     LFQQLLEIKKKILHVLASIEVEIEYPEDEETFLDAFKPEELKEILNELEILSESWSCE
                     KMYQEGARLILAGKTNAGKSSLFNAILKEDRAIVSEQHGTTRDWLESFISLDGIPARL
                     FDTAGLRETEDLIEQKGVLMTQELAKDADLLLYLIDGSIGISPEDSAFIEDILTKQSF
                     PLLLVLTKKDKTETNLFDVIKAMAALKFKHPNSMADALEIVHISSKTGEGISELSKKA
                     KALLSQKHASQRKQAGLGSLRQKKAVDEAIESITHALEVYEADYTIDALALDLENALE
                     SLAIITGEVSGDDVLDSVFSHFCVGK"
     gene            complement(17542..19677)
                     /locus_tag="PKW26_02880"
     CDS             complement(17542..19677)
                     /locus_tag="PKW26_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOQ92545.1"
                     /translation="MKNSSLRHLFFYFLAFFFSLSLFAQTNQNALYEQVLIGTKEGLF
                     QVNNYQTQRLWAEAGIHKIIYTDRYYFLTTKGVYESKDLQSFTSKNTGLPAHSVKTIE
                     NGEKIFSLLNVDLKDLASHPTKPEILVTATKDAVYISRDSAESWKSLGSSARTAGCKS
                     VAVCDLPRYNSSGAITGSELIVFMSHPIYGLSYIQADSANPKWIDITRGFDSLPNNPL
                     VDEIADILPVVSIDANGNQTIDVFLSQSFIPRLYSLDWKNKKAVPLWKGALVNASFDS
                     LVYENGALHFLEMGGFSTLNIAESKIQKNTAQTNQWFQSIRNQNLIAEAAFFKHSNDP
                     SRSVSLDELWMLYPNTIASSYSYKASGKKSLYLPVNQITGAGLQKHIQTIADNKLNSV
                     VVDMKDDYGMLRYKSKEAKILEKNGISRHAIDLEDFVNPLKEKDIYLIARIVVFKDKN
                     LYQWQGGKYAVWDSALKKPWQGIRGYSDILDENEIKTGTQTNYYDEFWVDPYSDEVWE
                     YNVAIAQELIERGFDEIQFDYIRFPTDGLNLSNASYRHHEKDMDKESALLSFLKYARS
                     NIQAPIGIDIYGANGWYRSGARTGQDVELMAPYVDVICPMFYPSHFEQNFLDYKPAAE
                     RPYRIYYFGTYRNAVIARNQIIVRPWLQAFYLGVSYDKLYYDKNYVKRQVFGVRDSIH
                     NGYMYWNNSGRYDDIEPDIGDDEAYPWTY"
     gene            19810..20910
                     /locus_tag="PKW26_02885"
     CDS             19810..20910
                     /locus_tag="PKW26_02885"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOQ92546.1"
                     /translation="MRKNKVEKFGILTSGGDAPGLNAAIRAVVRTAISSYGMKALGIE
                     HGYRGLIDGMAHELLLDDVSGILTRGGTILGTSRESPFKKLEFDPETGMNDVEKILAQ
                     YKKWELDALVVLGGNGTNTTGAHLAKAGLNIIGLPKTIDNDLVLTDTSFGFHTAVSVA
                     TEAIDRLHTTAHSHDRIMVIEVMGHKAGWLALYAGIAGGGDVILIPEIPYNIENIAKH
                     LKKRQKQGKIFSIVVVAEGAMNKEESMMDKKTFKKTRKEMKNSIGYRIAQELEEATGL
                     ESRVTVLGYLQRGGTPVSADRVLATQMGVEAAHMLYRGEFGKMVAMQEGRLLGVPLEE
                     VAGKIKMVEKTHPLVKAAREVGTCFGEKDEDE"
     gene            20897..21790
                     /locus_tag="PKW26_02890"
     CDS             20897..21790
                     /locus_tag="PKW26_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HOQ92547.1"
                     /translation="MKTSKTNYKCHFCEVCVGKGCTAELPGMGGVFESRNFVLNCAAW
                     DGFSLDSFNDKERKKIPRAKVRLAPITGALQNVGYHDEKAFYKDLTEFVFKAGFRLSI
                     GDGTPDDKLQFGIQAIQKLQEKKPKTKAAVFLKPYANEILFERIEWASPVAEIIGIDI
                     DAYNILTMRNLVNLEKKTASQMKEVQKKAGLPFAVKGVFTSADMDLVKELRPDIVVVS
                     NHGGRVETDIGSSAEFLARRGRELRNYCGEIWVDGGIRKERDVEIASFLGASEVLVGR
                     PFITALCRGDSAESLRNTFAR"
     gene            21883..23643
                     /gene="ettA"
                     /locus_tag="PKW26_02895"
     CDS             21883..23643
                     /gene="ettA"
                     /locus_tag="PKW26_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOQ92548.1"
                     /translation="MAKTYDDKKIIYSMDRVSRTYGTKTVLKDVSISYFYGAKIGVVG
                     ENGSGKSSLLKILAGVDTDFVGETHVSPGYTIGYLEQEPELEKGKTVKEIVSEGVKEI
                     TDLLQEFDEVNEAFGDPDADFDKLCNRQADIQEKLDALDAWNLDANLELAMDALRCPP
                     PEQSVDVLSGGEKRRVALCRLLLQKPDILLLDEPTNHLDAETVAWLERHLQSFPGTII
                     AITHDRYFLDNVAGWILELDRGEGFPYKGNYSGWLEQKKQRLALEEKGESDRQKALQK
                     ELDWIQMTPKGRQAKHKEHITKYEQLLNESGREKLRDTKITIPAGPRLGNLVVEAKDL
                     TKGYGERLLFDNLSFTVPAGAIVGIVGPNGAGKTTLFRLIADAAGQTVERLDGTEGGE
                     KPDAGELRVGESVKLTYVDQMRSLLDPEKTVWEQLSDGLDIIKLGAVDEKGRALNGAI
                     REVNSRAYCSWFNFSGSDQQKKVGVLSGGERNRLNLAMMLKQGANLLLLDEPTNDLDV
                     TTMRALEEAIESFAGCAMVISHDRWFLDRICTHILAFENNSEVRWFEGNWSDYAEWRR
                     KELGGEADRPHKTVYRKMTR"
BASE COUNT         6752 a         4183 c         5462 g         7377 t
ORIGIN      
        1 ggttttgtaa atatcggcta ttttaaattg gcttgggtcg actttgtttc ccgttcccat
       61 cgaagaaata tgtggaatgc tgagttctcg cgcccgttct ataatcaaga tttttgcact
      121 aatcgtgtct actgcatcaa taatataatc gcaaagggaa agttcagcct caagcttttc
      181 ttgcgcttct agggacgcta gagggctttt gacgaggttt tttttctctc catcgattga
      241 gttttgataa ctttcgtgtt tttctttcgg gaggtaaaaa agcttatgag ttgataccct
      301 tgcatgggga tttatgtcga gaatgcgctc ttttgcaagg tctacctttg cttggccgat
      361 ggtagaatgc agggcataaa tttgtcgatt gaggtttgaa ggagaaataa catcattgtc
      421 gatgaggata aaacttccga tgccagatcg agcaagggct tcgacgacga aggagccgac
      481 gccgccgaga ccaaaaacag caattgtttt agtgttcagt ttttgaacag ttcctggtcc
      541 aaggaggatt tctgttttat aaaagctccg ttctatattt tcttcgttat ttaatgcttt
      601 gagcataggc tttcatttct gcaagttctt ctgcaaagga gtcgatgcct tttttccaaa
      661 atgcctttgt ttgaatgtca aaacctgctt gcgcacatac ttcttcgcaa gaaaagcgtc
      721 cagtattttt tagaagttca atataggttg tagggaaact gctatctccc tttgctgtat
      781 tttctttgtg taccgcaaat aaggagcgtg caaagagaag accgaaggca taggggaaat
      841 tgtaaaaatc aagatcaggt ccataataat gtcccttaac agcccacatg aggtcgtgtt
      901 ttttatctgt taagcctgag ccataggttt tttcctgtgc ttctttcata agccgagaaa
      961 aatcttgtgc agagagctca ccttcttttt tatgttcaaa aactgaacgt tcaaaataaa
     1021 accgacttaa aatatcgacg aggacttggg ctgaatctga caggctactt tcgatgagct
     1081 ttactttttc aaaaccctgg gaccttccta tcgcatcgtg cattacaacc gtttctgcaa
     1141 agatactcgc tgtttctgcc agtgtcatag gatagtgtga aagcgaaaag tcttcgtctt
     1201 tgatacaatc aaagtgatag gcgtggccga gttcatgtgc aagagttagg atgtcggaga
     1261 aggtgccggt gaagtttgaa agaacgcgag gttctttgtg gtagggaaaa tccgtacaat
     1321 aggctcctcc aaccttgcct tgtcgcacct ttgcatcgat ccaattattt tcaaaggctt
     1381 tttgcgcaaa gtcggcgagg cttttggaaa attgtccgaa ggtttcgacg atatattttt
     1441 gtgcttctgc aaagctccat attttttctt ttgcattttc agagacaagg ggggcaaata
     1501 aatcataaaa ctcgcatttt tctaagccta aaagactcgc cttggtcttg aggtaatctt
     1561 gccaaaaagg gagggagtct tcgattgccg agatgagggc atctaagctt ttttttgaaa
     1621 ggcggtttgc aaagagtgct ttgtcgatgg gttcttccca cgagcgtttt ttgtttaatt
     1681 caagggttgc gccttttata ttatttaaac aggcggcaat ggcaatttct gagcttttaa
     1741 gaagggctct ttccttttca aaggcggttt ttcgcacatt ccgttcttgg gcataggctt
     1801 cattgcgcag ctcgttaaag gtttttcctg tttctgcatc gacaagggat gaaataattt
     1861 gctcttgtaa acggctccat gccgaggaag catagcgctg caaagagttt gcaagatttt
     1921 cttcctcacg agacatatag tgggaatcac cttcaatgta ttcgtttaaa ataaagctat
     1981 agcttgtata ttgtggataa gctgtgtaaa agttcggtaa agcttgttta tgttcggtga
     2041 gtatcttggt gaaaagaaag cctatatctt ggacttcaac aagggcttgc tctatttgtg
     2101 agaggttgtt taaaaatgcg gtatttgttg tgtcgcaaga atagatagca tgggcgtatg
     2161 cgtacatact ttgaaaaaga cttatggttt tattgtattt ttctaggtag gtattgagcc
     2221 aggatggaaa atcttccctg cttggctttg tggcaacgag agaggcaagc tctgaaagcc
     2281 cgttttttag attggttttt gcttcttgat gttctttact ttcgattgaa ggatagatac
     2341 tttcaagcga ccaaagagga atcgttttgt cagccatggt attaactcct ttgttttata
     2401 tgagttcttc taagtctagt tcacaagcaa agcaggggac gccaatgtcc cagttttcga
     2461 ggacttgggg atgtatttca ccaaagatac cgacttctgt ttcgccttta aatacgagag
     2521 cttgccgacc ttttataaat cgtggatcgt cgctttcttt taccttgtat tcatgaccta
     2581 aaaagtagag taaggcggca acttcgcttg ctccagtatt gaagtttgca tcttgcgaaa
     2641 ggctcacaaa accgaggttt tgtcttgtcc ttgtgccggt attttcctcg tcgcagagaa
     2701 aggcaacttt tccaatctca aaaatgttat gtggataaac tgcattggca gataccgctt
     2761 cagctgctaa aaggcttgga ataatagaag ggcgtacaaa ctgatagttt tcgctcatcg
     2821 ggtttgcaac ttctataacg ctatctgcgg aaatattcat acgatcgata taatcgcgct
     2881 tagagccgag gtagttaaaa atcatttctt ggtagccgag tccaaccatg aggctttttg
     2941 ccttgcgact aaataaggta aggggaagaa ggcgtccgat tgtaaaatca tgtggttttt
     3001 caggggtaaa aaaggaaagt ttttttccga ttgcaacgtc ttcgattatg tctacttcgt
     3061 gcaaaaagtc attgcgatag gcaggaggag tgagggtgaa aaggctttct ccttcttttt
     3121 cttctatgct tacgtggcaa tccatttttg taagcgcttc ttgcacttca tcttttgtaa
     3181 gattatgtcc taagagcgta tttatagcgg aaaggcgggc ctgtgttgtt ttttgaaagt
     3241 aaaagggcgt tgtaattgtc tttccatagc ctgtttcgta gggatgttcg acacgtacag
     3301 gaaggattgt gtaaccggca tcaaaaaagt cgcaggcgac gatgttcgtt gcaagcaaaa
     3361 gggagtccat atcggtgccg gtcatttcaa ccatgagatc gctgtcgcct acttcaacag
     3421 ctccgagttt cgcgctattg ataacgggag gcatagaaag tacctcatct tttgcatcta
     3481 caatgagggg aaagagtttt tcgttttcaa ttatccagcc aaaatctttg ccttttggat
     3541 gttctttgtt tatttcgcgc aagctcattt cactttctga agcaaggggg ataaaacgtc
     3601 ttttgtctgg gtcgcaagcc ttgtattgta ctggccattc gatgagctgt gagcgataga
     3661 cacccatcgc aatagacctt cttttgcgcc catagttcca gcagagtttt tcttgtgttt
     3721 gaataatgtc tacaagcatg tgttcatcta tgggtttacc agaaataaga aaggcggtca
     3781 taaaggggcg aatgtctttt aaggctgggt ctacttttat aaatctgtct gttttgtttt
     3841 ttaagccttc gtttgttgga aggtttttct tttcagttga taaaaaggaa gaataatcag
     3901 cagattttgc aagcccattg tgcatgcgta attgcctggc gatgcctgca gttgaccaaa
     3961 ggtcaggtcg atttgtatcg tttagttcta tttttatgct tcgctctgct tcggctaggg
     4021 aagggtcggg tttttcatcg agttcagctt ttgcacaggt gagtttttct tctaagctat
     4081 cgtaatcgta ttttgctcct aaaagattaa aaaataaggc ttcgtttact tcaatttttg
     4141 gcattgtttc ctcttttata tattcatttt gtattgattt atgtatagtt ctttcaatta
     4201 tagcaaagag aaaaatagtt gtctattata cagagctaaa tactttattc ttttattgaa
     4261 ttatgctagt tttttaactt aaggatgtat tatgaaaaga acagatatag aaaaaatttt
     4321 gattattggc tcgggtccaa ttgttattgg tcaggcatgt gagtttgact actcggggac
     4381 gcaggcctgt aaggctttgc gtgaattggg ctataaaatt gtcttggtaa attccaatcc
     4441 agcgacgatt atgactgatc cagttatggc ggatgcaact tatattgaac cattgaatgt
     4501 tgaaagaata gagcagatta ttgttaaaga aaagccagat gctcttttgc caaatcttgg
     4561 tgggcaaacg ggattaaatc ttgcttttga gttgagcaaa aaaggtattt tggaaaaata
     4621 cggcgtaaaa gttatagggg taaaccttga agcgattgaa cgtggtgaag accgacaaat
     4681 cttcaaggct gagatgataa aaataggcat agatacgccg cgctcagata ttgctcatac
     4741 aatagaagaa gctgaacaaa tagcagagaa tctttctttt cccgttgttg tgcgacctgc
     4801 ctatacgatg ggtggctctg ggggcggcct tgtttacaat agagaagaat tgcatacaac
     4861 ggttagccgt ggtttggagc tttctcttac gcatcaatgt ttaatcgaag agtctatttt
     4921 aggttgggaa gagcttgaag tggaagttgt tcgcgacaaa aaaaatcaaa tgatagcagt
     4981 ttgttttata gaaaatattg atgctgttgg tgtgcatacg ggagattcct tttgtaccgc
     5041 acctttttta accatcgata aggatttgga aagacgctta aaagagcagg cttttaaaat
     5101 agttgaaaat atcggagtca taggcggaac aaatgtgcag tttgcctaca accgaaaaga
     5161 agatcgcatt gcaattatcg aaataaatcc acgcacctct cgctcaagtg cgcttgcttc
     5221 aaaggcaacg ggctttccaa ttgcccttgt ttcggcaaag ctcgcttcag gtatgagctt
     5281 ggacgaattg ccctactggc gggatggaag cttggaaaaa tactccccag atggcgacta
     5341 catggttatg aagtttgcgc gctgggcttt tgaaaaattt aacggtgtta gcgattctct
     5401 tggtacgcag atgaaggcgg ttggagaagt catgtcgatt ggaaaaacct ttaaggaaac
     5461 ctttcaaaaa gcaattcgcg cccttgaaaa tggacgaagc ggtctaggct ttgcaaagga
     5521 ctttcacaaa aaatcggtcg acgagctttg cgatgcgctg aaagttgcca atagtgaacg
     5581 gtattttatg ctctatgagg cgattcgtaa gggcgcaagt ttaactcgcc tagaagaaat
     5641 tacctttata aaaaaatact atctcgagca aatacaggaa cttgttcagc tagaagaaga
     5701 gcttttgaaa aatcgcggaa tgcttccaag cgataggctc ttagaaaagg caaaaaaaga
     5761 tggattttct gataaatatc tagcgcaaat cctaaatatt cgagaaaaag atattcgaaa
     5821 aaagcgaaaa gagcttggca ttgttgaagg ctggcatgcc gttcctgttt ctggggttga
     5881 ggataagttt tattattatt cgacatataa tgctcctgat actgtttctg ttacagacaa
     5941 taaaaagaaa attatgattt tgggcggcgg tccaaataga attggtcagg ggattgagtt
     6001 tgattattgt tgtgttcacg catcttttgc gcttaaagac ttgggctatg agacaataat
     6061 ggtaaactgc aacccagaaa cggtttctac agattatgac acgagtgata ggctttattt
     6121 tgagcccgtt acaacagaag atgttttgca aatctatgaa aaagaaaaac ccgagggagt
     6181 tattgtgcag tttggcggac aaacacccct aaatattgca cgggagcttt ctgatgcggg
     6241 agtacgtatt ttgggaacaa gcatcgattc aatcgatata gcagaagacc gtgatttatt
     6301 caaaacgatg atggaaacac tcaatattcc tatgccagaa gcaggtatgg cgtcgaatta
     6361 tgaggaagcc ttggcagttg ctgaaaaaat tggctatcct ttgatgattc gtccttcctt
     6421 tgtgcttgga ggcaggggta tggaagttat ttatgatcaa aaaatgctca aagaatatat
     6481 cgaacgggct gttggagtaa gtccagatcg tccgctttta ctcgaccgct ttttaaaaga
     6541 tgcgctcgaa tgtgaggcag atgcgctttc agatagtgaa aatgtctttg tgccctcggt
     6601 gatggagcat attgagcttg caggagtgca ctcaggagac tctgcctgcg ttattccgcc
     6661 ggtatctatt acaaaagagc atataaaaac gattaaaaca tatacacaaa aaatagcaca
     6721 aagcttaaag gtttgtggct tgatgaatat gcagtatgcc atagagggtg ataaggtgta
     6781 tgtgcttgag gcaaatccgc gagcgagtcg aactgttccg cttgtttcta aggtctgtaa
     6841 tgtgcaaatg gcaaaacttg caacacggct tatgatgggc gagtccttgg cttctttgga
     6901 attaaaagaa aaaaagattc cgcactttgg tgcaaaggaa gcggttttcc ccttcgataa
     6961 gtttgacaat gtggaccctg ttttagggcc agagatgcgt tctacgggag aagtccttgg
     7021 catgagtgaa aactttgggc ttgcctttta taaatctcaa gaggctgctt ctgcaagttt
     7081 acctgactta ccagaagaag gtaaggtaaa aaaagtttta attagccttt cagagaaagt
     7141 tcatcaagcg aaggatgccg ttgaggttgg aaggggcttt cataaaatgg gcttttctat
     7201 tttggcaaca gagggaacag cagcgttttt ccaaaaagaa ggcattcctt gcgaggtaat
     7261 agcaaagctt aacgaaggtc gaccaaatgt gcttgatgtg atattgaaca aagaagtttg
     7321 tcttgcgata aatacaccga gtgcaaaaaa agatgttata aaagatgagg cagcgattcg
     7381 ccaagcggtt ataaagtaca aggttccgta tattaccacc ttggcagcgg caaaggcagc
     7441 ggtgcaagga attttggcat cttcaaaggg caagggctca gtaaagtcaa tacaggaatt
     7501 tcacaaagaa atacgctaag gcgaaaaagc ttttttagaa tgcttgtcta aaaagatttc
     7561 tctgtttata ctctagccta tgcatttata tcaaattttt attatagcac tagggctttc
     7621 tatggacgct tttgccgcag cgctttgtaa gggcttggca atgtccaaaa aaaacctaaa
     7681 aatagcctta ctcattgctg ttttttttgg aggctttcaa gccttgatgc ccttgttggg
     7741 ctggcttttg gcctttagct ttgcaacata tatagaagcc tatgaccact ggatagcttt
     7801 tgggatttta accattcttg gcggaaaaat gctttttgaa gcaataaaaa accgaaatga
     7861 aggctgccct gtgtattcaa ataaaaaaga agtgtataaa cgggaacttt ttcaactcgg
     7921 aattgcaaca agtatagatg cctttgcggt aggacttacc tttgcctttt ttgaagttgc
     7981 gatacttccc gcaatagcta taatcggtct tgtaaccttt gccttatcgc tccttgctgt
     8041 ttacctagga aacttttttg gggaaaaact taaaacaaag gcagaaattt ttgggggaat
     8101 tgttctcatt ggaatcgccc taaaaattct catcgaacat ctttttgttg cttaatatat
     8161 cttgccagta ttgaatttac cgtttataat tacattattc tcattttgac agagaatgat
     8221 attttgtcat gtttgataaa ggatcttgat ttatgcaaag tacaatgcca aagatgctac
     8281 gcgaggtagc aaagcagcat ccagatgttc cagctcagta ttttaaagat ggaacagggc
     8341 gttttcagtc ggtaaggtat tccgatttat ataaaacaat acttgatcta gcctcaggat
     8401 tgcttcattt aggggtaaaa cgaggtaatc atgtaggtat tatttctgat gatagaaaag
     8461 aatggcaaga agcaagcatg gcaattatgg gtctcggcgc tgctgatgtt cctcggggct
     8521 gtgatgcaac aaaagcagac ctttcctata ttttatcctt tgcagagtgt gaattttgtt
     8581 ttgtagaaaa tcaagcgcaa gtagaaaaat gtatttctat tcgtgaagca atcccaaatg
     8641 taaagacgct tattgttttt gatttggtgg aaaagacagc gctcgaagca gcaaaaaaag
     8701 cgggttttag tcttatacaa tataacgatg tgatagaaaa tggtacaaaa gagcgaaaaa
     8761 aaaatcctgg ttttgttgaa aaagaaatag acaaagggaa tgaagaagat atcgcttgta
     8821 ttatttttac ttctggaaca acaggggagc ctaagggcgt tatgctttgt catcgaaatt
     8881 ttttaacaca actcgatgaa ttaccagagc ttataactct tagccctgga gacaaggcgc
     8941 tttgtgtgct tcctgtgtgg cattcatttc agcgcttgtg tgagtatatg attctttttc
     9001 aagcaggttc tatgtgttac tcaaaaccga taggcagtat cttgcttgca gattttgcag
     9061 cactcaaccc gcaactgtta cctgcggtcc cacgtgtttt tgaagcagtt tatgagggag
     9121 tacttcgaac aatgagaaag ggcggcggta taaagtatct tctatttaaa ttttttgtag
     9181 gaattggtat tttacatagc aaaattgacc gattgttgct taataaaact gccttgttca
     9241 aaaaacgaaa tagatttttt gattggcttc ttcttgttct tccatggctt ttcttatatc
     9301 ctttgaaact tttaggttca gttttagtgt ttaagaaaat tcgagaaaaa ctagggaata
     9361 gttttcatgt aggcgtgtct ggtggtggag ctcttccgcc gattgtcgat gagtttttct
     9421 gggctgttgg cataaatatc gtcgagggct atgggcttac agaaacagct cctgttatat
     9481 cggtgcgatc tatgcacaat ccaacctttg gtacagtggg ttatgccatt cgaggtgttg
     9541 aggcaaagat tgtcgatgaa gatggaagag aattaggacc ggggcaaaag ggcacggtga
     9601 tgataaaggg tggctctgtc atgaagggct actataagaa gcccgattta acatcagagg
     9661 ttttaagtga agatggctgg ctcgatacgg gagatttagg cttgctgact atcgatgggc
     9721 aacttgtgct tcgcggtaga aaaaaagata ccatagtgct tcgtggaggc gaaaacgttg
     9781 agccactccc tctcgaaatg aggatgatgg agtcgcgctt tattgcccag gctgttgttt
     9841 taggtcagga tgaacgctat cttgccgcga tgattgtacc gagtcgtgac gatgttgaaa
     9901 gctatgcaaa agaaaaaatg atagatgttt cggtttttga aaaacttcta gaacatgatg
     9961 agatacaaaa actttttgaa acagagataa aagagctggt aaatgcaaag agcggtttta
    10021 aaatttttga acgcatttca cgatttgttc ttttgaaaaa accttttgag ccaggagttg
    10081 aactttctgc caagcaagaa gttatgcgct ataaactgcc agagctctat ccaaaagaaa
    10141 tgaagctttt atttaagtag cctttattat tcaataggtt caattgtatt tttctctcta
    10201 ggattgtgat tctcttcaac aaagttctct tcgtcaaaag agacgagcat atcgaggacg
    10261 gcaaaaaaga gaattataac ggttggtcct agaataagcc cgtttaagcc aaatattttt
    10321 aagccgccta aaattgcaaa aaagataaga aggggatgta cttttattct gtcttgcaag
    10381 aaaaatgggc gtaaaaaatt gtccagaaag ctgataaaaa atgcactcaa tactaaaaac
    10441 aaaatacctt taatcacaga atctgtaaat aataaaataa ttcccaaggg gaaccatata
    10501 agtccagctc caaaaagtgg aataaaagaa gaaaacataa gtaaaactga aaaaagaaga
    10561 gctgctttta taccaaaaat tttcattaga ataaaagcaa tgatgccttg atagagggca
    10621 acaagaaaat agccggaaag taaaccgcga attgtctcgg agaatttttt tgcgagtgta
    10681 tccatgtgtt ttgggtttat gggaatagct tttataagta gggtaaaaag ataggctccg
    10741 tcaagataga aaaaatagag ggcaaagaca acaaataata tagaaagtat aaaatttgcg
    10801 gtattttcaa ggagattttt tgaaatcata agcaaggtgt tgctatagta ttggattgtt
    10861 tgcagaataa aagcgcgaat atgtaagcca tccacttgag aaaataattc attttctatg
    10921 ccaaaatcat ttaagatatc tattgttttt tttataaagc ttgagtcaga gaaaaaggtg
    10981 gggtttgttc gtagaaataa ttcaatgtct ttgaaaacag caagtaattg ttgtgctaat
    11041 tgaattgtta aaagtaaaag aggtccaata ataagaatga gcgtggcaat tgaaaaaata
    11101 gctgctaagg catgtctttt aatttctaaa aaacgctttt tttgggtaat tttttgttgt
    11161 atttttttat agagcggatt taaaagaata tataaaaggg aagcccataa aataacatag
    11221 aaaaaggggt aaaaaatacc aacgagggca acaaaaaaaa gtgcaaatag ggcaaataga
    11281 aaaatggtat ttagatttgt tttttttaac attatattcc tttatgaatc gatgataata
    11341 gcaccggatt ttgaatcgat ttgagtccgc tttttttgaa gattgccttg ttttgtttcg
    11401 gctttttctt tgttttgctg aactaggtct cctgaaaata aagctgcatt ctcagctata
    11461 gtatacttgt ttttgacaaa aagcataagt ccttttttac cacaggcata gtgtcctatt
    11521 ttttgttttg ggaggcggat gggaagggag cttatgcgca aggcgctgtc gtctttgttt
    11581 ttttgcgggc atttgcttgc aaagacatag gaaagcacaa ttttttcttt tgcaagagct
    11641 gcatcgcgtg aaagtttttg gagtttttct ggtgcatttt cagcgttcat tgtaaagtgg
    11701 caccattttt tatctcgtgt gccaggcatc gggcaggctt cttcgtgggg gcagggagaa
    11761 agtatggaaa agttttgtcg taaaagtgag tcgcgaaaga ggcttataaa acgtgcactt
    11821 cgagggatgc caggttcaac aagaaggaca gaggcgtttt cgtcttttgt gtactgagaa
    11881 agtgtttttg catagagctt taccaactct tctaggggac gtctatcttc gagatacatt
    11941 tcgttaaaca tatttgcact gcatataagc ttggttttgt ttcgaattgt ttcgccaaaa
    12001 agccctttta ctttgataat attccaagct ctgccgcgaa gttttgcgac gatggaaagg
    12061 aagatatctt ctccgaggct gaggcttgtg ctcgaagggt ctaagcaata ccagtttagt
    12121 tttttgtctc gtagttcagg tcgagcgagg tagagagcaa taggaagggt gagagggcca
    12181 gagccaatat caaggcaata agtttcttct tctgtttgaa ggaggttttg aaaaaaatct
    12241 tttggaaagc gggcaaaaag agatgtaagg cgaaaaagat tccaccacat aaaataacgg
    12301 gtgtagctta tcatttcgac gcggctgttc atataggttt ttttacggct tgaacgctcg
    12361 cttgttagtt gtagagaaag ctgttgaata ttgtagggaa gactttgtaa atgcttgcta
    12421 ctcaatggtt ttatttcttg taggatacta tcaaagttat ccaagagaga aagggcatct
    12481 tttggcggac ggccgaacat gctctctcgt gtataatttt cttctttgtt tgtgtgcatt
    12541 atggtttcct gtgttttttt acaattaaaa aaagttcgat aagatctgtg cgtaattcgt
    12601 taaaagattg gcgaatatgc acagggcttc cgttttcatc aagaaggctt aagtcatcac
    12661 ttgcttcttc tatgagctta tttcttgctc cttcaagtgt gtatttttta tcttgtacga
    12721 gatgttttaa tcgaagcagg agaaaaaggt cctgttcagt atagactcgt ctgccactta
    12781 tgtttttttt tggtgcaata gaaggtatta tttcttccca atagcgaaga atatgtgcct
    12841 ttgtttgagt gagctcttca atatcgccaa ttgaaaactc ttttctcatt agctctccct
    12901 gtcttcccat ttttgacgac ttgctttgag ctcaaggata atgggaatat tgtcaaagcc
    12961 aaggtcgctg cgtattttat tttttagata tgtaatatag ctttgtggca ccacttctgg
    13021 acggcttgca aaaagtataa agctaatggg gtttgtgttt gattgcacgc agtagcgtag
    13081 cttaaattgg cttgccttgc tttgtggtgg tggatacgag cgaatccatt ctttgagtgc
    13141 aaggtttagt gaaggtgtgt caattttttt tgtaagttgc ccatagagtt tgagcgcagt
    13201 gtttagcaag gggtttatgc cttgtttttc aagggctgaa atacatagca cgggagccca
    13261 gatcatatgt ccaaagtgta tgcgtatatc ttcttcagct ttttttcgcg attgttttgt
    13321 gtcttcttgt aggtcccatt tatttaagat aaaaataata cctcgtcctc ggtcgtgggc
    13381 aagagagata atttttttat cttggtcggt taaaccttct tctgcatcca agatgtaaaa
    13441 aacaaggtcg cagttttgca aggtttttat agcacggtta acagaataat attctatgtc
    13501 ttcttttacc tttgtttttt tacggatacc agcggtatcg agtagatgga agtttttacc
    13561 tttgtaggag aaaaagcctt cgactacatc gcgtgttgtg cctgcataat cgcttacaat
    13621 ggatgcgctg ctttttgtaa ggacgtttga aagggttgac tttcctgtat ttggttttcc
    13681 catgatagaa acttttattg gtttttcttc gcttccctct tcgactgcag aaaagtcaag
    13741 gcgagagata atttctttgg ccaaggcggt gattccgtcg ccgtgggatg cagagatgag
    13801 gtgcagggat tgaaagccaa attgcataaa gttccatgct tcttcttctt ttctgccgcc
    13861 ttctgttttg tttacagcag cgattacctt atcccagtag gggcgtaaga gagcgatgaa
    13921 ttcttcatct tctgcggtta tttcaccgac ttctaaaaca agaacgatga ggtcgcagtt
    13981 ttttagggtt tcaagggttt tttctacaac gagctcgtcc attatggctt catcgctgcc
    14041 atggtcgcgg tcgagtttaa aaccgcccgt gtcgatgagt tttactggat agcccaaaat
    14101 aaaggcattt ttttctacag ggtcgcgggt tacgccgggt ttcgagtcaa caatagcaag
    14161 tcgtctgttt aaaaatcgat taaaaagagt cgattttcct acatttgggc gtcctgcgat
    14221 gacaattcgc ggtctgtttt tatagtcact gtccatagaa tatgggctgt cgtatgtttt
    14281 ttgatgttta ggtgtgaaag actttgaaaa ttgctttttt tgattttgtt tttttttcat
    14341 atatacctta taaagacttg agggaaatag tttctaaaaa agcttttact tcagaaattt
    14401 tatttgctcg catactgagg gagcgaatgc ctcgttttaa aagcgctttt atttcgtctt
    14461 ctcgggcggc catttctcca caaactgata tggggatatt gtgctttttt gctgccttgc
    14521 ttgtcatttc gatgagggcg agtacagcgg gatgcatttc ttggtagaga gctgaaacac
    14581 ctgtgttttc tcgatcgacg caaagagtgt actgggttaa atcatttgtt ccaatagaaa
    14641 aaaagtcaac aatttctgca aaatcttctg caaggattgc tgcagcgggc gtttctatca
    14701 taataccaag gggaacatct tttttaaagg ctatgttttt ttctgtgagc tcttttttta
    14761 tctcatcaat gagagccttt gtttcgagga cttgctcttt atgagtgatg agtgggagca
    14821 tgatggaaag attgccgtca atgcttgctc gaagaagtgc acgtaattgt gttttgaata
    14881 tatccttttg accgagacag aaacgaatag cgcgatttcc caaaagagga ttttcttcta
    14941 tttctggttt tatatacttt gttttttttc cattttttga gattgagttt tctgtaacag
    15001 aaaggacctt atctcctccg gcatcaaggg tgcgaataac aacagatttt tcgcccataa
    15061 ttttgagcac ttctgaataa acttggtatt gttttgtttc gtcgagtaag tctgaagggc
    15121 gttcggcatc catgaataaa aactctgttc gaaaaagccc tattccttca gcaccatgtt
    15181 taagcgctaa atctgcttct tcgacagagc caatgtttgc aaagaggttt atctcaacct
    15241 tatcttttgt ttttcctttt ttgtttatat actgtgctag aaggttttct cgttcttcgg
    15301 cttctttttt gagtttttga tatttttcta ttgttttaag gtcaggggag atacagacgc
    15361 ttccttcatt tgcatctaaa ataatggtat cgccgtgaga aatatgttca ctcagtttat
    15421 gtatgccaaa aataaagggg attccgtaac ttcttgcaag gatggctgtg tggctggaaa
    15481 gacctccgtc agaaagtaag agtcctttaa tgcctttttg gaagagagat atggcaaagg
    15541 agccagaaat attttgtgaa acgacgatag agttagcgtc aatgtcttgg atattttcta
    15601 ttttattgcc gaggatgcgg tcgattacct tgccaaaaac atcgagaata tcatctgctc
    15661 gttgtgaaag gtattcgtcg ttggtatggc gtaaaagaga ggcgctttcg tttacaacgc
    15721 tgtcaacaat aaaatctacg ttgtagcttg agttgtcgaa atttagtttt atttgattta
    15781 taaattcttg gtcattgagc atgagaagat aggtttctat aagttttttt tcatcaggat
    15841 tatttgtctg taagagaagt gaaaaatctt taatagtgag ctccaaggct ttctggaatt
    15901 ttttccattc ggcttctttt tcagattgag aaattcttgt tttttctact tgtttttttt
    15961 tcgattgcac aataaagtgt gctttttcta tggcaaggcc ctttgacgct gaaattcctg
    16021 ataaaatatt catagactta ctatagcaat ctcaaggcaa taagtcaaag ggaaagctat
    16081 tttcctacac aaaaatgcga aaaaacactg tcgagaacat catctccgct gacttcacct
    16141 gtgataatgg cgagagattc gagggcgttt tctaaatcca gggcgagggc gtcgatggtg
    16201 tagtctgctt cataaacttc tagggcatgt gtgatagatt ctatcgcctc atcgactgct
    16261 tttttttgtc gtagagagcc aagtccagct tgttttcttt ggcttgcgtg tttttggctt
    16321 aaaagagctt ttgccttttt gctgagttca gaaatcccct ctcctgtttt gcttgaaata
    16381 tgtacgattt caagagcatc ggccattgag tttgggtgtt tgaattttaa agctgccatt
    16441 gcttttataa catcaaagag atttgtttct gttttatctt tttttgtgag aactaaaagc
    16501 aagggaaagc tttgttttgt taaaatatct tctataaaag cgctatcttc cgggcttatt
    16561 ccgatggagc catcgatgag gtagaggagg aggtctgcgt ctttggcgag ttcttgggtc
    16621 atgaggacgc ccttttgctc gatgagatct tctgtctctc gcaaaccagc tgtgtcaaaa
    16681 agtcgtgcag gaatgccatc gagggagata aagctttcaa gccagtcacg agttgtgccg
    16741 tgttgctcgg agacaatggc acggtcctct tttaagattg cattgaaaag actcgatttt
    16801 cctgcatttg ttttcccagc aagtataagg cgtgctcctt cttgatacat tttttcacaa
    16861 gaccaagact cggaaagaat ttctagttca ttgagaattt ctttgagctc ttctggttta
    16921 aaggcatcta aaaaagtctc ttcatcttct gggtattcta tttcaacttc gatggaggcg
    16981 agaacatgga ggattttttt cttaatttca aggagctgtt gaaaaaggga gccagaaagg
    17041 cgccctgccg cacgacttga agagtcttgt gtttttgatt ctataatttc tcgaattgcc
    17101 tctgcctttg tgaggtcggt ttttccattt agaaaggagc gaaaagaaaa ttctcctcgc
    17161 tcagcttctg aaaagccctt tgagagtaaa agcgcataga tgctgcggac aacggagata
    17221 ccgccatggc aaaaaatttc gaccatgtct tctccggtga aacttttggg agctctgtag
    17281 accgcaagga taacttcgtc aacttttttt tctccgtcga caatccagcc gtagagcgtt
    17341 gtatttccag ctgctttttg aagtgcttct gggcgagaaa aaatcgctgc gacaagagat
    17401 ataacttctt ttcccgtaca acgaatgaca gccaaggcgc ttggtgcaag gggtgttgcg
    17461 atcgctgcga ttgcttgttc agggatatag gttttatttt ctaacatcta gggacctcct
    17521 aaaaaaacga gggtaatcta atcaatacgt ccaagggtag gcttcatcat cgccaatatc
    17581 tggttcaata tcgtcgtatc gaccgctatt attccagtac atatagccat tgtggatgga
    17641 atcgcgcacg ccaaagactt gccgtttgac atagttttta tcgtagtaga gtttgtcata
    17701 agaaacaccg agataaaaag cttgtagcca aggccgtacg ataatttgat ttcgtgcaat
    17761 gacggcattt ctataggttc caaaataata aatgcgatag gggcgctctg ctgctggttt
    17821 ataatctaaa aagttttgct caaagtggct tggataaaac atagggcaaa taacatcgac
    17881 atagggtgcc ataagctcta catcttgtcc tgttcgtgcg ccacttctat accaaccgtt
    17941 tgcaccgtaa atatctatcc cgatgggcgc ttggatattg cttcgggcat actttaaaaa
    18001 cgaaagaagg gcgctttcct tgtccatatc tttttcatga tgacgatagc ttgcatttga
    18061 aagatttagt ccgtcagtag gaaagcgaat gtagtcaaac tgaatttcgt caaaaccgcg
    18121 ctctatcaat tcttgtgcaa tagcaacatt gtattcccag acttcatctg aatagggatc
    18181 aacccaaaac tcatcgtagt agtttgtttg tgttcctgtt tttatttcgt tttcatcgag
    18241 gatatcagaa tagccacgta tgccctgcca tggttttttt agggcagaat cccaaacagc
    18301 gtattttcca ccctgccact ggtagagatt tttatctttg aaaacaacaa tgcgagcgat
    18361 tagataaata tctttttctt tcaagggatt tacaaagtct tctaaatcga tggcgtgtcg
    18421 gcttatgccg tttttttcga gaattttagc ttcttttgat ttatagcgaa gcatgccata
    18481 gtcgtctttc atgtctacaa ctacggaatt gagtttattg tcagcaattg tttgtatgtg
    18541 tttttgtaaa cctgcaccgg taatttgatt tacaggtagg tagaggcttt tttttccgct
    18601 cgccttatag ctatagctgc ttgctatagt gttcggatag agcatccaaa gttcatcgag
    18661 ggaaacagaa cggcttgggt catttgagtg tttaaaaaaa gcggcttcag caataagatt
    18721 ttgattacga atactttgaa accattgatt tgtttgggcg gtattttttt ggattttgct
    18781 ttctgctata ttgagtgtag aaaaaccacc catttctaaa aagtgcaggg caccgttttc
    18841 atagacgagg gaatcaaaac ttgcatttac caaagcgccc ttccaaagtg gaactgcttt
    18901 tttatttttc cagtcaaggg agtagagccg tggaataaaa ctttggctca aaaaaacatc
    18961 tattgtttga tttccgtttg catcgataga aacgacgggt aaaatatctg ctatttcgtc
    19021 aacgagggga ttatttggca agctgtcaaa accacgggtt atatcaatcc attttggatt
    19081 tgctgaatct gcttggatat acgaaagccc ataaataggg tgggacataa agacaatgag
    19141 ctcgcttcct gttatggcgc cagaagagtt gtagcgaggg aggtcacaga cggcaaccga
    19201 tttgcaacca gctgttcgag cgcttgaacc gaggcttttc catgactcag cagaatctct
    19261 agaaatatag acagcatcct ttgttgcggt tacaagaatt tcgggttttg taggatgact
    19321 tgcaagatct tttaagtcta catttaaaag gctaaatatt ttttctccgt tttctattgt
    19381 ttttacagag tgagcaggaa gccctgtgtt tttgcttgta aagctttgta aatctttact
    19441 ttcatacaca cccttagttg ttaaaaaata atagcgatct gtgtagataa ttttatggat
    19501 gccagcttcg gcccaaagtc tttgtgtttg gtagttattt acctgaaaga gtccttcttt
    19561 tgtaccgata aggacttgtt catacaaagc attttgattt gtttgggcga aaagagaaag
    19621 gctaaaaaaa aaggctaaaa aataaaaaaa aaggtgccga agcgatgagt ttttcatagc
    19681 gatatactag caaattttta ttgattattc cagtcgttaa atagacgtgg agctaaagct
    19741 gtgttaaagt gatataaaat agagcgtagt gagagttctg gtttctaaaa ttttttagtt
    19801 aggatgtgta tgagaaaaaa taaggtcgaa aagtttggaa ttcttacttc tggaggagat
    19861 gctcctggct taaatgccgc aattcgagct gttgttcgaa ctgcaatttc tagctatggc
    19921 atgaaagcct tgggaataga gcatggatat cgtggcttaa tcgacggcat ggcgcatgag
    19981 cttttattgg atgatgtttc aggtatattg actcgcggag ggactattct cggaacgtct
    20041 cgggaaagtc ctttcaaaaa actcgagttt gaccctgaaa caggcatgaa cgatgtggaa
    20101 aaaattcttg ctcagtataa gaaatgggaa ctcgatgccc ttgttgtgtt aggcggaaac
    20161 ggaacaaata caacaggcgc gcatttggct aaagcagggt tgaatataat cggtcttcca
    20221 aaaacaatcg ataatgactt ggttttaaca gatacaagtt ttggttttca taccgctgtt
    20281 tcggttgcaa cagaggcaat agaccgcttg catacaaccg cgcatagtca tgatagaatt
    20341 atggtaatcg aagtaatggg gcataaggct ggttggcttg ccctttatgc aggaattgct
    20401 ggaggtggcg atgttatatt gattccagaa attccgtata atatagaaaa cattgcaaag
    20461 catttgaaaa aaagacaaaa acaaggtaaa attttttcga ttgtcgttgt agctgaaggc
    20521 gcaatgaaca aagaagaatc catgatggat aaaaaaacct ttaaaaaaac gcgtaaagaa
    20581 atgaaaaatt ctattgggta taggattgca caagagttgg aagaagcgac aggtttggag
    20641 tctcgcgtaa ctgttttggg ttatttgcaa cgaggaggga ctcccgtttc ggcagatcgc
    20701 gttcttgcga cacaaatggg agttgaagcg gcacatatgc tgtatcgggg agaatttggt
    20761 aaaatggttg ccatgcaaga aggaaggctt ttgggagttc ctttggaaga agttgcaggg
    20821 aaaataaaaa tggtggaaaa aacccatcca ttggtaaagg ctgcgagaga ggtaggaacc
    20881 tgctttggag aaaaagatga agacgagtaa aacaaattat aaatgtcatt tttgtgaagt
    20941 gtgtgtgggc aagggctgta cagctgagct tcctggcatg ggtggtgttt ttgaaagccg
    21001 caactttgta ttgaactgtg ctgcctggga cggtttttct ctcgattcct ttaacgataa
    21061 ggagaggaaa aaaatccctc gtgcaaaggt tcgccttgct cctataacgg gagccttaca
    21121 aaatgtgggc taccatgacg aaaaagcctt ttacaaagat ttgaccgagt ttgtttttaa
    21181 ggcaggtttt cgcttaagca taggcgacgg cacccctgac gataaacttc agtttggcat
    21241 tcaagctatt caaaaactgc aagagaaaaa acccaaaaca aaggccgctg tttttttgaa
    21301 accctatgca aacgaaatac tttttgagcg cattgaatgg gcgagtcctg tagcagaaat
    21361 cattggtatt gatatagatg catataatat tcttacgatg cgaaacttgg tgaacttgga
    21421 aaaaaagaca gcaagtcaaa tgaaagaagt gcaaaaaaaa gcaggtctcc cctttgcggt
    21481 aaaaggtgta tttacctctg ctgatatgga tttggtaaaa gaactgcgtc ctgacattgt
    21541 ggtggtatcg aatcatggag ggcgagtaga aacggatatc ggtagctcgg cagaatttct
    21601 tgcccgaaga ggtcgagagt tgcgaaatta ttgcggtgaa atatgggtag acggcggcat
    21661 acgaaaagaa cgagatgtag aaatagcaag ctttttggga gcaagcgaag ttttggttgg
    21721 tcgccccttt atcaccgccc tttgtcgtgg agatagcgca gaaagcctac gaaacacttt
    21781 tgctcgctaa tatttctgga gctgaaaacc tgtctgtatc gataaacctc acgcccctcc
    21841 tttttacttg tttctttttt gcttctttac tacaatgaaa ccatggcaaa gacatatgat
    21901 gataaaaaaa taatttattc gatggatagg gtttctcgta cctatggaac aaaaactgtt
    21961 ttaaaagatg tgagtatatc ttacttttac ggtgcaaaga taggcgtcgt tggtgaaaac
    22021 ggctctggaa agtctagcct tttgaaaata cttgcaggtg tcgataccga ctttgttggc
    22081 gaaacgcatg taagtcctgg ttataccata ggctatcttg aacaagaacc tgagttggaa
    22141 aaaggcaaga cagttaaaga aattgtttca gagggtgtaa aagagataac agatctcctg
    22201 caagaatttg atgaagtaaa tgaagccttt ggcgaccccg atgccgactt tgataaactg
    22261 tgcaatcgtc aagctgacat acaagaaaaa ctcgatgccc tcgatgcctg gaacctggat
    22321 gcaaaccttg aacttgcgat ggatgccctt cgctgtccac cacccgagca aagtgttgat
    22381 gtactttctg gaggagaaaa aagacgagtt gccctctgtc gccttctctt acaaaaacca
    22441 gatattttac ttcttgatga accgacaaac cacctcgatg cagaaaccgt tgcctggctg
    22501 gaacgccact tacaaagctt ccctggaacc attatagcca ttacccacga ccgttatttt
    22561 ttagacaatg ttgctggttg gatacttgaa ctggatcgcg gcgaaggatt tccttacaaa
    22621 ggaaattatt ctggttggct tgagcaaaaa aagcaaagac ttgcccttga agaaaaaggc
    22681 gagagcgata gacaaaaggc gcttcaaaaa gaacttgact ggatacagat gactcccaag
    22741 ggaagacagg cgaaacataa agaacatata acaaagtatg agcaattatt gaatgaatct
    22801 ggccgagaaa aactgcgaga tacaaaaatc acaatacctg caggtcctcg acttggaaac
    22861 cttgtcgttg aagcaaaaga ccttacaaag ggctatggag agagactttt atttgacaac
    22921 ctatcgttta ctgttcctgc tggtgcaatt gttggaattg tcggcccaaa cggagctggt
    22981 aagacaacgc tctttcgcct cattgcagat gccgcgggac aaacagtgga gcgactcgat
    23041 ggtacagaag gtggtgaaaa accagatgcc ggagaacttc gcgttggcga gagcgtaaag
    23101 ctgacctatg tagaccaaat gagaagcctc ctggacccag aaaaaaccgt ctgggaacaa
    23161 ctctcggatg gacttgatat tatcaaactt ggtgcggtgg acgaaaaagg acgggcactc
    23221 aacggagcta ttcgtgaagt aaattcacga gcctattgct cttggtttaa tttttctgga
    23281 agcgaccaac aaaaaaaagt aggcgttctt tcgggaggag agcgtaaccg cctcaacctt
    23341 gcaatgatgc tcaaacaagg agcaaacctc ttgttgctag acgagccgac aaacgacctc
    23401 gacgttacca caatgcgtgc tctcgaagaa gcgatagaaa gttttgccgg ttgcgccatg
    23461 gtgataagcc acgaccgctg gtttttggac aggatttgta cacatattct tgcatttgaa
    23521 aacaatagcg aagtgcgatg gtttgaagga aactggagcg actatgccga gtggcgccga
    23581 aaagaacttg gaggcgaagc agaccgccct cataaaaccg tgtatagaaa aatgacccga
    23641 taaaagtagg ctcggtatag gctgtgaata aagacaatgt tgacagcctg cgccaaaact
    23701 tgctatacta aaaaagcttg ggggtgtagc tcatctggct agagcgcttg catggcatgc
    23761 aagaggtaag gggt
//
LOCUS       DAOSSW010000018        23438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_2353_length_23438_cov_6.531754, whole genome shotgun sequence.
ACCESSION   DAOSSW010000018 DAOSSW010000000
VERSION     DAOSSW010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 23438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23438
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2353_length_23438_cov_6.531754"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..>1733
                     /locus_tag="PKW26_02900"
     CDS             34..>1733
                     /locus_tag="PKW26_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOQ92549.1"
                     /translation="MQTIEDLKQYTFKALLENSVKKFEKEKALSLVNGKPITYRELYE
                     KSLVISNLLKNSGLKKGDKVAIYSSSMPQWGIVYFGIVNAGFIAVPLLPDFSANEIET
                     ILEHSETKVVFVAEKMKARLDTIKEDLIEILFKIEDFSLFYGNIDKKQLSLNPLSPEE
                     RKMLKEIKIQEEDIASIIYTSGTTGRSKGVVLTHKNIVYNAVQCQTVHRVHRRDRALS
                     FLPLSHVYEFTIGFVMQMLNGSAVYYLGKPPTVTSLLPAFRKIRPTLVLSVPMIIEKI
                     YKNKVLPELTKTDLTKKLYQKPFFRKILHRIAGKSLNKTFGGKINFFGFGGAKVDPEV
                     ELFMKEARFKYAIGYGLTETAPLIAGSGPKNTIPGTIGPVLEGVELAILDPDPETGIG
                     EIVAKGENIMQGYYKDEALTKSVFTTEEDSTGPGWFKTGDLGIIDTKNRLSIKGRLKN
                     MILNSAGENIYPEDIEFILNQHPLVTESLVVEDGNGLVALVQLDNEKLEKEKGEGFTK
                     IAGNTINEIKNDLLYLQEEVLSEIHFFVNQKVNRISKLSAVRYIQEFEKTASQKIKRY
                     "
     assembly_gap    1734..1833
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1834..3404)
                     /gene="murD"
                     /locus_tag="PKW26_02905"
     CDS             complement(<1834..3404)
                     /gene="murD"
                     /locus_tag="PKW26_02905"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOQ92550.1"
                     /translation="MNKSKPVKPKKDSHKSSVPNQSKLNISENLISNSSLFHNYPSFC
                     PISSLADIKDKKVLVMGLGLHGGGEASVRFFLKHGAFVTITDLKTEIELESTIKSLKN
                     DSSLNLDKADFVLGRHDIRDFEEADLVIKNPAVKYEGNIYLEKAKHIESDISLFLRFT
                     KSPIIAITGSKGKSSTASAIHFGLQQAGFHSFLGGNITISPLHFLEKTNENTPVVLEL
                     SSWQLSDLRGREILKPKIAIITKIVPDHLNWYGTMENYIEDKKLINKNQDSSSFTICS
                     ADSWGDIFASGTEAQVIRYSSKPFQGHIAKGAWLDTHSKGYVTRNTALSIKMGTEDLK
                     FIKKISSNSKISLLAKTAIPILDNLLVPGIHMKENMLIASLALVLMGVSPLKTAKIMA
                     KFPGVPHRLEYFHKKTLQNLPTGGTEDLKNLKKAAKTSFLFYNDSAATVPDATIAALS
                     SFTDPIHLISGGTDKNLDFTELAKHLPKAKSLYLLAGSGTELLIKELDKSSVPYFGPF
                     PSLDALLARLKEQIT"
     gene            complement(3397..4299)
                     /locus_tag="PKW26_02910"
     CDS             complement(3397..4299)
                     /locus_tag="PKW26_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92551.1"
                     /translation="MKLDFYKLHLGGNSFLLIDIGKHSISTNALSPIARQICDRRYGV
                     GASACIFLYPDNFVRIFLPNGKEHFHSYDAFFCAARYSFDSGYFLKTANKDRITLKTP
                     LGDRGFYIISPREFKISLGSPFSLTNGARIDKNSKHSIETIRINNKPVCISGFHIKDD
                     IVAVNAKEVNISSFFDLYLKINNVFTENKTYLVYYREVAKEAMSIRTIKRGPSTSCIS
                     AAAALVSSVLSGNPETASVFIFEKGSPSLYTQEINLLEDFDDSRKLTILWENETNELF
                     AIGSAGYTFEGSFDYRDGNIKNYE"
     gene            complement(4296..5402)
                     /locus_tag="PKW26_02915"
     CDS             complement(4296..5402)
                     /locus_tag="PKW26_02915"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOQ92552.1"
                     /translation="MKIVILYGGKSSEHEISLLSASSVVRNINLKKHKVSLIAITKKG
                     QWFLQDDSEISRIQNDKNAVLRISENNEAVFIAPGLGGDRGFKTASQTIPCDVVFPVL
                     HGAFGEDGTIQGLLEIAQVPYVGCGVMASSLTMDKEKTKQVWQTQNLPVVPYMTVKKH
                     EFLTNDTKTKIIEETEQKFTYPVFVKPCNAGSSVGTAKVRDRGFLEKALEEAFSWDEK
                     VLIEPFIDAREIECSVIGNQTPIVSVLGEIIPTHEFYDYEAKYNDPNGANFFIPARIS
                     SEETEKIKVLAQKAYEAVDASGFSRVDFFIDKQSNEIYLNEINTIPGFTSISMFPKMC
                     EAAGIPYKTIIERLLDLAVERFKARQALNTGLHM"
     gene            complement(5407..7056)
                     /locus_tag="PKW26_02920"
     CDS             complement(5407..7056)
                     /locus_tag="PKW26_02920"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOQ92553.1"
                     /translation="MDREKHLDTLLSSIKILEISSPTLNPLIQNLVFDSRNVTKGSLF
                     FALPGNTVHGNTFIQEAIEKGAVAIIYQDEISSSLIQTHKANVVMVKVQDSRYAMSPI
                     ADSFYDSPSSKLKVIGVTGTEGKSTTTYLIWQLIQLLGAKAGFISTVQTSFGDRAFDN
                     TEHLTTPEAPIIHYSLAKMLENNCEYAIIESSSHGLSKKTNRCGDIHYDVGIVTNVTH
                     EHLEFHGTREQYAFDKANLFRSLDLFSHEKTVLGLKSSVPTFGVINADDSVAFSFAQN
                     TEQACFFFSDKSSVPLKEQNIESKTLFSASNIISSEKKLSFTLTNENLTKQSVEVETN
                     LIGTFNVHNILASIITVSKIMGKSIEEVAKTCQKLIPVKGRMSSVDKGQNFEVIIDYA
                     HTPSSFNTIFPPIRDRAQGKIISVFGSGGNRDTKKRPEQGAIAARYSDIVILTDEDPR
                     TENSMDILTMIAEGCKNPYNFSVSRGTEVYCKADSTSLFLIPDRKKAIRKAFSLASKN
                     DIVLLLGKAHENSIIYADHIMPYDEIEEAISALAEMGYKKG"
     gene            7168..7656
                     /locus_tag="PKW26_02925"
     CDS             7168..7656
                     /locus_tag="PKW26_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOQ92554.1"
                     /translation="MRKRRILKENAMYHVGARINNKEMLLKSNELKALFLAVVKRAKK
                     KYIFQLTNFVVMENHIHMIIKPGKNQNLSRIMQWVLSVFAVICNKKLGRTGHLWGERF
                     FSKIISSLQEYIKISDYIDNNPVKAGLASGEKVWKFSGKYHRLVKKYSILEKPETLAL
                     LF"
     gene            7776..9473
                     /locus_tag="PKW26_02930"
     CDS             7776..9473
                     /locus_tag="PKW26_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOQ92555.1"
                     /translation="MKDIVLLGDEAIALGAIHAGLSAGFGYPGTPSTEILEFLIKNKE
                     NVSKSDQLESSVPIAQWCSNEKTAYESALGASFAGKRSIVTMKHVGLNVAADPFMNSS
                     LVGINGGLVLIVADDPSMHSSQGEQDSRFFADFAMIPCFEPTTQQEAYEMMFEAFEIS
                     EKNKVPVMMRVVTRLAHSRAVVRPFDVPLHIKQANLLKANKLEWMLLPALARKNYEKM
                     IEKQELFQKWSNSNKWNTLQMSKNKSDFAIITSGLGKNYYLENIKDYAEYLKALSPSQ
                     ELPSHLHIATLPLPLGKIQELTQNAQTLLIIEEGMPVIEKQLKGLLPQKITVLGKLSG
                     HLPRTGELNPDFVRKALSLEERKSITSNPDIAKLTTDLPGRPPQLCRGCPHADSYSSL
                     NKAVSVLKEKAGDDNVVIMSDIGCYALGALSPYNAIESIVCMGASLGMARGAAQVGVK
                     YAFGVIGDSTFLHSGIQNLVDAVSTKTPMTAIILDNSIVAMTGCQETIVPSNKLESLV
                     VGLGVEPEHVRVLSTFPNKIEENTKILLEEAEYRGVSVILLVRECVESLRIKNKAKK"
     gene            9503..10075
                     /locus_tag="PKW26_02935"
     CDS             9503..10075
                     /locus_tag="PKW26_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOQ92556.1"
                     /translation="MKYDILLAGVGGQGVLSIAAIIAQAAMAEGLHVRQSEVHGMSQR
                     GGGVQAHLRISDSEIYSDLIPKGGADMILSMEPLESLRYLEFLCPQGVLITSSVPFEN
                     ITNYPDISELHKKIKKIPHSVLVDSKSIASVAGNLRSENMVLVGAALNHIPIQKKTVE
                     TAVCNRFGSKDPRLVDINCTALAMGTEVLA"
     gene            10075..>11312
                     /locus_tag="PKW26_02940"
     CDS             10075..>11312
                     /locus_tag="PKW26_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOQ92557.1"
                     /translation="MSIEIPFWDKPVETLDRKEIEELQLIGLKKQVEHALNTSFYSKR
                     FKEYKILGTEDFKTLSDIRRLPFTTKNDLREAYPFGMLCVPKEDLVRIHASSGTTGTP
                     TIIYHNKKDLDMASDTMARSLVAAGCKKGDIVQNMMTYGLFTGGLGFHYGAERIGMTV
                     IPTSAGNTLRQIAFMKDFGTTVTHATPSYLLHLHQAIENSDYKRSDFKWRLAVTGAEP
                     HSEELRQKIQEKLGIEVYNCYGMSELNGPGVAFECIFRNGLHIWEDRYIFEIIDPDTL
                     EPLPDGEIGELVVTILHRDSMPLIRYRTRDLTRVIPEPCPCGRTHRRLARFTGRTDDM
                     LIINGVNVFPSQIEEVLLKKSGIGANYLILVEKAGLLDKLTVQTEVTPELFADDARVL
                     NALRDSLQTEISALISIHPK"
     assembly_gap    11313..11412
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11702..12310)
                     /gene="thrH"
                     /locus_tag="PKW26_02945"
     CDS             complement(11702..12310)
                     /gene="thrH"
                     /locus_tag="PKW26_02945"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HOQ92558.1"
                     /translation="MYITCLDLEGVLVPEIWIAFAEETGIEELRLTTRDISDYNVLMN
                     KRIDILKAHKLKLKDIQNTIEKINPMDGAKNFLDNLRELSQVIILSDTFTEFALPLMK
                     KLGMPTIFCNSLTVSEDGFISGYKLRQDNGKYHAVKGFQSMGYKIIASGDSYNDIEMI
                     KQAEKGFLFRSPTHIQEEHKEFQSFVEYDELYQAIKREIQEK"
     gene            12565..13530
                     /locus_tag="PKW26_02950"
     CDS             12565..13530
                     /locus_tag="PKW26_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92559.1"
                     /translation="MKKICVFFLALSFFYGFAENAASGDFNLSVDLAYYPLSEKISGE
                     THFSPLSAPYSGVELRTVASFTWTIPTPFSENPLVSGNNLKIKTALELSPVSFVPELS
                     FSFTPIAFLIFSTGIKAGSGWTFTPLSVDGMAQYDNVSSSYNPLSPFSSLYYNFWAEA
                     LFQFDLAALYPGEWNHIALVATYKPYYEALSKGGEGGNPWYWQGSGEKANGWKYYSSI
                     IFAYQMPLLLQTIAVQTEFEGFYDASSFASAYQAWDPCFAKISISPLVNLEFSKSQNL
                     IILFGFSSRRSFSAQTSHLQNDLEKTTIGREWFFNRIALSYSIRF"
     gene            13686..14507
                     /locus_tag="PKW26_02955"
     CDS             13686..14507
                     /locus_tag="PKW26_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOQ92560.1"
                     /translation="MENELITPYLSQLKKYPLLSADEEKKLANSIENGDMRARNLLVQ
                     SNLRLVISIAKKYLHYKVSLSDLLQEGNIGLLIAATKFRSSFNTRFSTYAYLWIQQYM
                     LRFIRNKNPAIQIPHRKEAEIRRIRASQDHLHQHLGRKPNADELSLYLGLSEKEIKNI
                     LDHEFSLKSLDLIVDITSETTFGQFLTADTPSPEEDFLVTEKREIVLNIMQELPKKER
                     DVIASRYNLAHIDSKNTLRFVGSKLGLSTETVRQIEKRALKKIRSKALERGFALS"
     gene            14542..>15055
                     /locus_tag="PKW26_02960"
     CDS             14542..>15055
                     /locus_tag="PKW26_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92561.1"
                     /translation="MNAENKRKKIKRKKKSSPRRTLLFFAFVLFSCLLLIVFSSIFIS
                     KDVEQFSDKQEDTMLYEEKKAITEVLPDMQESSVPQKEYVEKTLTSSSNKTQSSVPNA
                     PKADLEVQKGALVKSSVPQVEKNIDRSQVLAESSVPQKKRGSIILVLDDGGHNISQLQ
                     PFLNLPFPLTI"
     assembly_gap    15056..15155
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15156..15791
                     /locus_tag="PKW26_02965"
     CDS             <15156..15791
                     /locus_tag="PKW26_02965"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOQ92562.1"
                     /translation="HNISQLQPFLNLPFPLTIAILPALAYTKESASLTRKAGKEVMLH
                     QPMQAINLSVDPGPSAILPNMTEAEIRAILQKNVSEIAPIVGMNNHEGSLITADANAM
                     SVVLDFCRDTALLFLDSRTNVETKVSFVAKEKKMQIWERNIFLDNTPDRENITSMFQS
                     GLEIAEKNGYVIMIGHVWSGSHLAQILDELGKKAIQDGFVFTTLSALELNQ"
     gene            15805..16845
                     /gene="tsaD"
                     /locus_tag="PKW26_02970"
     CDS             15805..16845
                     /gene="tsaD"
                     /locus_tag="PKW26_02970"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOQ92563.1"
                     /translation="MKILGIESSCDETACAIVEDGKKILSNVVATQIPFHEEYMGVVP
                     EIASRKHIEWILPVVKKALQDASLNISEIDGFAVANRPGLMGSLLVGLSFIKTLAWSQ
                     NKPFIAVNHMLAHLYASHLAVDISYPYLGLLVSGGHSIICKVLDFDTIEVLGTTIDDA
                     VGEAFDKVAKFYNFGYPGGVAIDRLAKEGNPRAASFPIPVLNKGEKKEHWYDVSYSGL
                     KTAAINQIDQFWHEGYEKTPENIAAAFQETAVNILLTRLFRAVEDTGIKTIVAGGGVA
                     ANSRLRALLAERDDLHCIFPPLKLCTDNGAMIAGLGYHYLKRGESSPLTTTASPRVTE
                     FKRGLKVQRDRA"
     gene            complement(17012..17572)
                     /gene="cyaB"
                     /locus_tag="PKW26_02975"
     CDS             complement(17012..17572)
                     /gene="cyaB"
                     /locus_tag="PKW26_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOQ92564.1"
                     /translation="MFEIELKASLTNRKEVEEKISQFAEYLGKTEKSDVYWTQSRVGH
                     PQKVRLREETNKPLRVNYKKKEIRSGQIEVNDEYEFTIGAGLDKKEAEKEKLSFETFL
                     SSAGFSPSMVKHKDTKRWNFEDTLIEVSYVDKLGDFIEIEILSETADTERTIEAHKKL
                     LRTLEKAGVPEENIETRYYSELLENV"
     gene            complement(17565..18647)
                     /gene="ilvE"
                     /locus_tag="PKW26_02980"
     CDS             complement(17565..18647)
                     /gene="ilvE"
                     /locus_tag="PKW26_02980"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOQ92565.1"
                     /translation="MAFSLQMYPITYMAKYNSETEKWEERWIESDTITFDELNALSPE
                     KKEAVLAARNDLKLPGVSYTSQYGFGCFEGMKAFPTKDGRVAIFRPDQNAKRFANSMR
                     GLKAPAFPEDLYINASKEFVKRNAVLGYVPSYNAEWEKDSYASASAVYMRPFMNTEGA
                     IGVGISVEPYVIICGTTVSSYFKGGNTKAITTERIRATEHGTGWIKCASNYVISALAK
                     REAEDAGYMEVIFLDTSRKYVQEGSSCNIFFKLKDGTLITPELGDTILPGITRNSTIT
                     LANQLGIKVEERLVTIDEVMKNAVECFVTGTAAGLTPIESITHEGREAVFNNREPGEV
                     ATQIQKILKGGQYGSVEDTNNWNYYV"
     assembly_gap    18685..18784
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18817..19425
                     /locus_tag="PKW26_02985"
     CDS             18817..19425
                     /locus_tag="PKW26_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOQ92566.1"
                     /translation="MKKGMKVFLIVLAVVLLLIFSLYRFWAGNYNAMVQKGETVDAAW
                     AQVQNQYQRRFDLIPNLVNTVKGYASHESEVFTNIADARSRAGGVLQVSDEVLNNPES
                     FARFQQAQNELGSALQRLLVITENYPELKADQNFLALQDQLEGTENRIGTERKRFNDV
                     VRDYNTFIRQFPRVIIANMSGFTAKQYFAAGTEAQSAPQVQF"
     gene            19477..20184
                     /locus_tag="PKW26_02990"
     CDS             19477..20184
                     /locus_tag="PKW26_02990"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOQ92567.1"
                     /translation="MLSKKTKKMIKKLNLSKKDFDDIQAAVEKEEKKTSGEIALALIR
                     ESDSYSFWELFFSVIVGGFVFSLLLPLSPFFEKFLTSFLWTYSSWQLPAIIGLITFFV
                     IALLFNIANIPSIDRFIIPYVVRHRAVYLRALRHFVESGVYATRDHSGILIFISVMER
                     EVRILADIGLAEKIEQEKWNTIAQELSAAFKANAVKEGLEKAIHDCGLLLQEHFPVQE
                     DNPNELADGLVVLEVAE"
     gene            20181..20987
                     /locus_tag="PKW26_02995"
     CDS             20181..20987
                     /locus_tag="PKW26_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOQ92568.1"
                     /translation="MIRVNNAGFRKHSFIVLFFVLAVSVFSLSVPPMQGAVNDWAGIL
                     SASEKNELETFLNATNDQTSVQVAVLTLPSLEGESLEAFSMKVSEAWKLGQKEKDNGV
                     LLLVSMEEKKIRIEVGYGLEDRLTDIKSGLIIRNIIAPAFQSGQFGKGIVEAAKTIVG
                     IATDNEEIISKKINKTDSDDSIVGFIIFIIFVLFMTGALGRRGRGGCGGGLGNLFWLH
                     LLFGGSGRGGSSFGGRSGGSFGGGSSRGGLGGGFRGGGGSFGGGGASGGW"
     gene            21101..22210
                     /gene="ychF"
                     /locus_tag="PKW26_03000"
     CDS             21101..22210
                     /gene="ychF"
                     /locus_tag="PKW26_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOQ92569.1"
                     /translation="MAINCGIVGLPNVGKSTIFSALTSAPAEAANYPFCTINPNIGIV
                     DLPDARLDFLVKTLKPKNKVPTTVEFVDIAGLVKGASKGEGLGNQFLAHIREVGMIAQ
                     VVRCFKNDDIIHVNNKIDPVDDMLTINMELAFADLATLEKALERAQRASRVQAKDEQK
                     KALSLIAVLEKIKPLLENGESVRKAGLTDEEKALVYECHFITLKPQLYVCNVDEDGMQ
                     NGNEYIDAVRAQAEQEGAKLVVLCGKFEAELADMDSEEEKAAFLAELGLEESALSVLA
                     REAYDLLGLRTFFTAGEIECRAWTIRKGDRAPKAAGVIHSDFERGFIKAEVYSYDDLV
                     SYGSEAEIKAKGRYRVEGKEYIVQDGDIIFFKFNV"
     gene            22303..23310
                     /locus_tag="PKW26_03005"
     CDS             22303..23310
                     /locus_tag="PKW26_03005"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92570.1"
                     /translation="MKKDTKLFKMLAFSFVLVLFFASCHNLSSSFAGRGYRQGSLSIT
                     SWNIQTFFDTQTSGREYSDFIGTKLWNDAVYVKRLERLCQTMEALSSDLFIFQEIENE
                     AVLVDIVNYLKTQGNPALAYSHAVFIPSRDDVLGCAILSRYPIISCTTHQIDYRFEGK
                     KQPDTRPLMEVLVQAHFSKPLRVYTSHWKSKSGGAEQSEFWRDAQEALIARRIRETGD
                     QLYVFAADCNRDLSEFAQDGHYIVLDTEGQRLKTQSPYLFDAFADEDGTYFFQKEWNK
                     IDHFFIGEALHILSFCIERKGLHVDKDGIPYRFSIWNGQGYSDHLPISCVVSEKMNKN
                     D"
BASE COUNT         6958 a         4082 c         4694 g         7304 t
ORIGIN      
        1 ttgagagttg ttgcaattat ttgaggagca atcatgcaaa caattgaaga cttaaaacag
       61 tatacattta aagccctgtt ggaaaactct gtaaaaaaat ttgaaaaaga aaaagctctg
      121 tcccttgtta acggtaagcc aattacatat cgagaattgt atgaaaaatc attagttatc
      181 tcgaatttac ttaaaaacag tggacttaaa aaaggggata aggttgctat ttatagttct
      241 agcatgccac agtggggtat tgtgtatttt gggattgtaa atgctggttt tatagctgtt
      301 cccttgcttc cagatttttc agcgaacgag atagagacta ttttagagca ttctgaaaca
      361 aaggttgttt ttgttgctga gaaaatgaaa gcaagattgg ataccatcaa agaagatcta
      421 atagaaatct tattcaaaat agaagatttt tctttatttt atggaaatat tgataaaaaa
      481 cagctttctt taaatcctct gtccccagag gaacgaaaaa tgcttaagga aataaagata
      541 caggaagaag atatagcatc aattatttat acttcaggaa ctacggggcg ttcaaaaggt
      601 gttgtcttaa cacataaaaa cattgtgtat aatgctgtgc aatgccaaac agtacatcgt
      661 gtacatagga gggacagagc tctgtccttt ttacctctat ctcatgtgta tgagtttact
      721 attggttttg ttatgcaaat gcttaatgga agcgctgtat attaccttgg gaaaccaccg
      781 acagttacga gcttacttcc tgcgtttaga aaaattcgcc caacgcttgt tctaagtgtc
      841 ccgatgatta tagaaaaaat ctataaaaat aaggttctac cggagttaac aaaaacggat
      901 ttaacaaaaa aattgtacca aaagcctttt tttagaaaaa tcttacatag aattgctggg
      961 aaatcgctca ataaaacctt tggaggcaaa attaattttt ttggcttcgg tggtgcaaaa
     1021 gttgacccag aagttgagct ttttatgaaa gaagcacgat ttaaatatgc tattggttat
     1081 ggtcttacag aaactgctcc attaattgct ggatctggtc ccaaaaatac tattcctggc
     1141 acaataggtc ctgttttaga aggcgttgag cttgcaatcc tcgatcctga cccagaaaca
     1201 ggtataggtg aaatcgttgc aaagggcgaa aatattatgc agggctatta taaagacgaa
     1261 gcacttacaa aatcagtctt tacaacggaa gaagatagca cgggtcctgg ttggtttaag
     1321 acaggagatt tggggataat tgatacaaaa aatcgtttgt ctattaaagg gcgactaaaa
     1381 aatatgatat taaattctgc aggtgaaaat atttatcctg aagatataga atttatttta
     1441 aaccagcacc ctcttgttac cgaatcgctt gtagttgaag atggaaatgg gctcgttgct
     1501 ttagtacagc tcgataacga aaaactggaa aaagaaaaag gtgaaggctt taccaaaata
     1561 gcaggcaata caataaacga aataaaaaac gatttattgt atttacaaga agaggtacta
     1621 tctgaaatac atttttttgt gaaccaaaag gtaaatagaa tttcaaaact ttccgcggtt
     1681 cgctatattc aagaatttga aaaaactgca agtcagaaaa taaaacggta tttnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctcgtaa tttgttcttt taaccttgca
     1861 agaagcgcgt cgagggatgg gaaagggcca aaatagggga cagaggattt atctagttct
     1921 tttattaaaa gctctgtccc cgagccagcg agaagataga gcgattttgc cttgggcaaa
     1981 tgctttgcca gttctgtaaa atctaagttt ttatcagttc caccagaaat aagatgtatt
     2041 gggtcagtaa aagagcttaa tgctgcaatt gtcgcgtctg gaacagttgc agctgaatcg
     2101 ttataaaaaa gaaagctcgt tttggcagct ttttttaaat tctttaaatc ctctgtccct
     2161 cctgtaggaa ggttttgcag tgtttttttg tgaaaatatt ctaatcgatg tgggactcca
     2221 gggaatttcg ccattatttt tgctgttttt agaggactga ctcccatcaa gaccaaggca
     2281 agcgatgcaa tgagcatgtt ttccttcata tggattccag gcaccaatag attgtcaaga
     2341 atagggattg cggtttttgc tagtaaagat atttttgaat tgctagagat ttttttgata
     2401 aattttaaat cctctgtccc catttttatt gaaagggctg tgtttcttgt cacatatcct
     2461 ttactatgcg tatcaagcca agcgcctttt gctatatggc cttgaaaagg cttgctacta
     2521 tagcgaataa cttgagcctc tgtccccgaa gcaaaaatat ctccccagga gtcggcagag
     2581 catattgtaa agctggagga atcttggttt ttattgatga gttttttgtc ttctatgtag
     2641 ttttccattg ttccgtacca gtttaaatgg tctggaacaa tttttgtgat aattgcaatt
     2701 tttggtttta gaatttctct tcctcgaaga tctgaaagtt gccaacttga aagctctaaa
     2761 acaacaggag tgttttcatt tgttttttct agaaaatgta gtgggctgat ggttatattc
     2821 ccacctaaaa aagagtgaaa gccggcttgt tgcagtccaa aatgtatagc actcgccgtt
     2881 gaagattttc ccttgctacc tgtaatggca ataataggtg attttgtaaa gcgtagaaat
     2941 aaagagatat cgctttctat gtgttttgct ttttcgagat atatattccc ttcgtatttt
     3001 accgctgggt tttttataac aagatccgct tcctcaaaat cccgaatatc atgacgccct
     3061 aaaacaaaat ctgccttatc aagatttagt gaactatcat ttttaagaga ttttatagtt
     3121 gactctaatt caatttctgt ctttaggtca gtaattgtaa caaatgcacc atgttttaag
     3181 aaaaaccgaa cagaagcttc tccccctcca tgcaatccaa gtcccattac aagcactttt
     3241 ttatctttta tatctgcgag gctcgaaatt gggcaaaaac tgggatagtt atggaataaa
     3301 ctgctatttg aaatgagatt ttcggaaata ttgagcttgc tttggttggg gacagaggat
     3361 ttgtgtgaat cttttttggg ctttacaggc ttagatttat tcatagtttt ttatatttcc
     3421 atctctgtaa tcaaaacttc cttcaaatgt atatccggcg ctgcctattg cgaaaagttc
     3481 attcgtttca ttttcccata agatagttaa ttttctagaa tcatcaaaat cttctaaaag
     3541 atttatttct tgcgtgtaaa gagaagggga gcctttttca aaaataaaaa cagaggcggt
     3601 ttctgggttt ccagataaaa ctgacgatac gagtgcggcg gcagcactaa tgcaagaggt
     3661 tgacggtcct cgttttattg tgcgaataga catagcctct tttgcaacct ctctgtaata
     3721 aactagatag gttttatttt ctgtgaaaac attatttatt ttaaggtaga ggtcaaaaaa
     3781 agacgagata tttacttctt tggcatttac cgcaactata tcatccttaa tatgaaaacc
     3841 tgaaatacaa acaggcttgt tattgatacg tattgtttca atagaatgtt tgctgttttt
     3901 gtcaatacgt gccccatttg taagagaaaa aggactacca agagaaattt tgaactctct
     3961 aggagaaatt atataaaatc ctctgtcccc aaggggagtt tttagtgtta ttctgtcttt
     4021 attagctgtt tttagaaaat agcctgaatc aaaggaatag cgcgctgcac aaaaaaatgc
     4081 gtcataacta tgaaaatgtt ctttgccatt tggtaaaaaa attctaacaa aattgtctgg
     4141 gtagagaaaa atacatgcgc ttgcaccaac tccgtaacgt cggtcgcata tttgccttgc
     4201 aattggggac agagcatttg ttgaaatgga atgttttcct atgtcaatga gtaaaaaact
     4261 attgccaccg aggtgtaatt tataaaaatc tagtttcaca tatgtaaccc tgtattgagt
     4321 gcttgtcgag ccttaaatct ttcaactgct aagtctaata aacgttcaat tatggttttg
     4381 tagggtatgc ctgcagcttc gcacattttt ggaaacatac taatcgatgt aaacccaggg
     4441 atagtattga tttcgtttag ataaatttcg ttactttgct tatcgataaa aaaatcaact
     4501 ctcgaaaagc cactcgcatc aactgcttca taggcttttt gcgctaggac tttgattttt
     4561 tcagtttctt cactgcttat tcgagctggg ataaaaaagt ttgctccatt ggggtcattg
     4621 tatttggcct catagtcata aaattcatga gtaggaatta tctctcctaa aactgacaca
     4681 ataggtgtct gatttccgat aactgagcat tcaatttctc tcgcatctat aaaaggctcg
     4741 attagcactt tttcatccca agaaaaagcc tcttcaagtg ctttttctag aaatcctctg
     4801 tccctcacct ttgctgtacc tacagagcta cctgcgttgc agggtttgac aaaaaccgga
     4861 taggtaaatt tttgctctgt ctcttcaata atttttgttt ttgtatcgtt cgttaaaaat
     4921 tcatgttttt ttaccgtcat atacggtact acaggaagat tttgcgtttg ccaaacttgt
     4981 tttgtttttt ctttatccat ggtaagcgag cttgccatca caccacaacc aacataaggc
     5041 acttgtgcta tttctaacaa gccttgtatt gtcccatctt caccaaaggc tccgtgtaaa
     5101 acaggaaaaa caacatcgca gggaattgtt tgagatgctg ttttaaatcc tctgtccccg
     5161 ccaagacctg gtgcaataaa aacagcttcg ttgttttctg aaatacgtaa aacagcgttt
     5221 ttatcgtttt gtattcggct aatttcagaa tcatcttgta aaaaccattg tccttttttt
     5281 gtaatagcga ttaaggacac tttgtgtttc ttgagattga tatttcgtac aaccgaagat
     5341 gctgacaaaa gagagatttc gtgttcgcta ctttttccac catataaaat aacaattttc
     5401 attatattat ccttttttat agcccatttc agctaaggca gagattgctt cttcgatttc
     5461 gtcataaggc atgatatgat cagcgtaaat tattgagttt tcatgcgctt ttccaagcaa
     5521 taatacgata tcgtttttac ttgcaagaga aaacgctttt cgaattgctt tttttctgtc
     5581 gggaattaaa aataggcttg tagaatccgc tttgcaataa acctctgtcc ctcttgagac
     5641 cgaaaagtta taaggatttt tgcagccttc agctatcatg gttaaaatat ccatggagtt
     5701 ttccgttcta gggtcctcat ctgttaaaat tacaatatcg ctgtatcgtg ctgcgatagc
     5761 tccctgttct ggcctttttt tcgtatctct atttccacca gaaccaaaca ctgaaattat
     5821 ttttccctgt gctctgtccc taatgggagg aaaaattgtg ttaaacgagg acggcgtatg
     5881 tgcataatct ataataactt caaaattttg tcccttatca acagaactca tgcgcccttt
     5941 aacaggaatc aatttttggc atgtttttgc tacttcttct atggatttcc ccattatttt
     6001 cgataccgta ataatagaag caaggatgtt gtgcacattg aaggtaccga ttaagtttgt
     6061 ctctacctct actgattgtt tggttaaatt ttcattcgta agagtgaagc taagtttttt
     6121 ttcagaagaa ataatgttag aagcagaaaa aagtgttttt gattcgatgt tttgttcttt
     6181 tagagggaca gaggatttat cagaaaagaa aaaacaagct tgttctgtat tttgtgcaaa
     6241 agagaaggca acagaatcat cagcattaat taccccaaat gtggggacag aggatttaag
     6301 acctaagact gttttttcat gcgagaaaag atctaaagag cgaaataagt ttgctttatc
     6361 aaaggcatat tgctctctag tgccatgaaa ttctaagtgt tcatgggtta cattagtcac
     6421 aatgccaaca tcatagtgaa tatccccaca acggtttgtt tttttggaca aaccatgaga
     6481 agaagattcg ataattgcat attcacagtt attttcaagc atttttgcaa gagaataatg
     6541 aataataggt gcttctggag ttgtaaggtg ctcggtgtta tcaaaagctc tgtccccaaa
     6601 agaggtttgc actgtcgata taaaaccagc tttagcacct agtagttgta ttagttgcca
     6661 aatgagatat gttgtcgtgc ttttcccttc cgttccagtt acaccgataa ctttaagttt
     6721 cgatgacggg ctatcgtaaa aagaatcagc gataggactc atagcgtaac gtgaatcttg
     6781 cacttttacc atcacaacat ttgctttgtg ggtttgaatt aaggaagaag agatttcatc
     6841 ttgataaatt atagccactg cccctttttc aatagcttct tgtataaatg tgttgccatg
     6901 gacagtattt cctggtagtg caaaaaataa acttccttta gttacgttcc tagaatcaaa
     6961 aaccaggttt tgtattaaag gatttagcgt aggggaagaa atctcgagta tttttattga
     7021 gctcaatagt gtatctaaat gtttttctct atccatagtg gtggaattag tataacgaaa
     7081 aaaaaaagaa aaaaaaaggg gacagaggaa ttttttttta attaaattta aatgcaaaaa
     7141 agtcttcaca aaaaccaata ataaggtatg agaaaaagaa gaatattaaa agaaaacgcc
     7201 atgtatcatg ttggagctcg tatcaataac aaagaaatgt tgttaaaatc aaacgaatta
     7261 aaagctttgt ttttagcggt cgttaagcga gcaaagaaaa aatatatttt tcagctaaca
     7321 aattttgttg taatggaaaa tcatatccat atgattatca agccaggaaa aaatcaaaac
     7381 ttgtctcgca ttatgcaatg ggttttaagt gtttttgctg ttatatgcaa caaaaagctc
     7441 ggtcgcactg ggcatttatg gggagagcga tttttttcga aaattatttc ttccttgcaa
     7501 gagtacatta agatttcaga ttatatcgac aacaatccgg ttaaagcagg tttggcaagt
     7561 ggcgaaaaag tatggaagtt ttccggaaaa tatcatcggc tggtaaaaaa atatagtatt
     7621 ctcgaaaagc ctgaaacctt agccttgctt ttctaaaacg cgcaccaggg aaaactttct
     7681 agagctaaaa tctaccccga ctcatttcta tattatatct ctttacacct cagttaagtc
     7741 ttgttatact aaactttata attaaagagg taaaaatgaa ggatattgtt ttattaggtg
     7801 atgaagctat agctctgggg gcgattcatg caggtttaag cgctggattc ggttatccag
     7861 gaaccccatc aacagaaata ttagaatttt taataaaaaa caaagaaaac gtgagtaaat
     7921 ctgatcaatt agaaagctct gtccccatag cgcagtggtg ttctaacgaa aaaactgcgt
     7981 atgagtcggc tttaggtgct tcttttgctg gaaaaagaag cattgtgaca atgaagcatg
     8041 ttggtctcaa tgttgccgct gatccattta tgaattcttc gctggtaggt attaacggtg
     8101 gtcttgtttt gattgttgcc gatgatccta gtatgcattc aagccaaggt gagcaggatt
     8161 cccgcttttt tgctgatttt gcgatgatac cttgttttga accgacaact caacaagaag
     8221 cttacgagat gatgtttgaa gcctttgaaa tttctgaaaa aaataaggta cctgtaatga
     8281 tgcgcgttgt tacccgtctt gcacatagtc gcgctgtggt tagacctttc gatgtgcctc
     8341 ttcatataaa acaagctaat ttgttgaagg ctaataaatt agaatggatg cttttacccg
     8401 ctttagctcg aaaaaactat gaaaaaatga tagaaaagca agaactcttt caaaagtggt
     8461 ccaattctaa taaatggaat accttgcaga tgagcaaaaa taagtccgat tttgcaatta
     8521 ttaccagcgg cttaggtaaa aactattact tagaaaacat aaaagattat gctgaatatt
     8581 taaaagctct gtccccatct caggaactac ctagccatct tcatatcgcg actcttccac
     8641 tgcctttagg aaaaatacaa gaacttactc aaaacgctca aacgctcctt attatagaag
     8701 aaggaatgcc tgttatagaa aaacagttaa aaggcttatt accacaaaaa attacagttt
     8761 tagggaagct ttcagggcat cttccgcgga caggagaact caatccagac tttgtaagaa
     8821 aagctctgtc cctcgaagag agaaaatcta taacaagcaa tcccgatatt gccaaattaa
     8881 ctacagatct tcctggtcgc cccccacaat tatgtagggg atgtccgcac gctgattcat
     8941 atagctcctt aaacaaggct gtttctgttt taaaagaaaa ggctggggat gataatgtag
     9001 tgattatgtc cgacattggt tgttatgccc ttggtgctct gtccccctac aacgcaattg
     9061 aatcaattgt ctgcatgggt gcctctcttg gcatggcgcg aggtgctgcg caagtcggtg
     9121 taaaatatgc ctttggtgta ataggagatt ccacgttctt gcattctggc atacaaaatc
     9181 ttgtcgatgc agtatctaca aaaaccccga tgacagcaat tattttggat aattccattg
     9241 tagcgatgac gggttgccaa gaaacgattg ttccctcaaa taaactcgag tctttagttg
     9301 ttggtttagg tgttgaacct gaacatgtac gagttctttc tacttttcca aataaaatcg
     9361 aagaaaacac gaagattttg cttgaagaag cagaatatcg aggagtatct gtcatactat
     9421 tggtaagaga atgcgttgaa tcgcttcgaa ttaaaaataa agcaaagaaa taacaattta
     9481 tttaccgtat agaggagata atatgaaata cgatatatta ttggctggag ttggtggaca
     9541 aggcgtctta tccatcgcag ctattatcgc ccaagccgca atggctgagg gcttacacgt
     9601 tcgtcaatca gaagtccacg gcatgagtca acgtggcgga ggcgtacaag cacatttacg
     9661 catctctgac agcgaaatat atagcgattt aataccaaaa ggcggggccg acatgatttt
     9721 gtctatggaa cctttggaaa gtctacgcta tttagaattc ctctgtcccc aaggagtctt
     9781 gattacaagc tctgtcccct ttgaaaacat tactaattat ccagatatta gcgaattaca
     9841 taaaaaaata aagaaaatac cacattccgt tttggtggac tctaaatcaa tagcatctgt
     9901 ggctggaaat ctacgttcag agaacatggt cttggttggt gccgctttaa atcacatacc
     9961 catacaaaaa aagacagtag aaacagctgt ttgcaatcgt tttggtagca aagatcctcg
    10021 gctcgttgat attaattgca ccgctcttgc aatggggaca gaggttttag cctaatgtca
    10081 atagaaattc ctttttggga taaaccggta gaaaccttag atcgcaaaga aatagaagag
    10141 cttcagttaa ttgggctaaa aaagcaggta gagcacgctc tgaataccag tttttacagt
    10201 aaacgattta aggaatacaa aattttgggg acagaggatt ttaaaactct ctcagatatc
    10261 cgtcggcttc cttttacgac taaaaatgat ctacgtgagg catatccttt tgggatgctt
    10321 tgtgttccaa aagaagacct tgttagaatt catgcctcga gcggtacgac agggacgccg
    10381 actattattt atcacaataa aaaagacttg gatatggcaa gcgatactat ggcgcgttct
    10441 ttggttgctg ctggttgcaa aaaaggcgat attgtccaaa atatgatgac ctacgggctt
    10501 tttactggtg gcttgggatt tcattacggg gcagagcgta ttgggatgac tgtaattcca
    10561 acaagcgcag ggaatacgct tcggcaaatt gcgtttatga aagattttgg cacgacggtg
    10621 acacatgcga caccgagtta cctacttcat ttgcatcaag caatagaaaa ctcggactat
    10681 aaacggagtg attttaagtg gcgcttagcg gtaacaggag ccgaaccaca ttctgaagag
    10741 ctccgtcaaa aaatccagga aaaacttggt atcgaggtat acaattgcta tgggatgagc
    10801 gaattaaatg ggcctggcgt tgcttttgag tgtatatttc gaaacgggct tcatatttgg
    10861 gaagatcgat atatttttga aattattgac cccgatacct tagagcctct tcccgacggt
    10921 gagattgggg aactagttgt aactatttta catcgggatt ccatgccctt aattcgttat
    10981 cgaacgcgag atctcactcg tgttattccc gagccctgtc cctgcggaag aacgcatcga
    11041 aggctcgcac gttttactgg ccgcaccgat gatatgctta taatcaatgg tgtaaatgtc
    11101 tttccgagcc aaatcgaaga agtccttctt aaaaaaagcg gtattggtgc gaattatctc
    11161 attcttgttg aaaaagcagg cttactcgat aaacttaccg tacaaaccga ggtaacgccc
    11221 gagctttttg cagacgatgc gcgggtgtta aatgcattga gagatagcct ccaaacagaa
    11281 atctcagcac tcatatcgat acatccaaaa atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nntgagagat agcctccaaa cagaaatctc agcactcata tcgatacatc
    11461 caaaaattga gctaaaagaa cctggttcca tgcctcttgc ggaaggaaaa gcgaaacgtg
    11521 tcgacgatct acgcccaaaa agctaagacg ggggacagag gatttcaaaa aaatgaatct
    11581 tatttgaatt tatctgtaaa ttaaaactaa ctttggggac agaggattct tatacgtgta
    11641 gcttgtactt caaattcact cttgagggac agaggattta aaatataatg aaatctatct
    11701 gctatttctc ttgaatttct ctttttatcg cttgatatag ttcgtcgtat tctacaaagc
    11761 tttgaaattc tttatgttct tcttgaatat gggtaggaga acgaaacaaa aagccttttt
    11821 ctgcttgttt aatcatttct atgtcattat aggagtcgcc cgatgcgata attttgtatc
    11881 ccatgctttg aaagccttta actgcatgat attttccatt atcttgtcgt aatttgtagc
    11941 cagaaataaa accgtcttct gaaacagtaa gagaattgca aaaaatggtt ggcatgccga
    12001 gttttttcat aaggggcagg gcaaattcag tgaaggtgtc ggagagaatt attacttgag
    12061 atagctcgcg caaattatct aaaaagtttt ttgctccatc cattgggttt attttctcaa
    12121 ttgtgttttg aatatctttt agttttagtt tatgtgcttt taggatgtct atccgtttat
    12181 tcataagcac attataatcg ctaatatctc ttgtcgtgag gcgcaattct tctattccag
    12241 tttcttcagc aaaggcaatc caaatttcag gcactaaaac gccttctaaa tccaagcaag
    12301 ttatatacat actattttcc ttcatttttt atgttcgacc agtttaccaa aaatagacta
    12361 ttcatgcaag gaatcttggg agacagagga ttttcctttt tggtatttat gctgctcatt
    12421 gtttattgag ggacagagga tttaaaaaaa atgaactata tctgaattca tcattaaatt
    12481 aaaacaagct ttagggacag aggatttaaa atttgacaaa acacttcttt tacatcatac
    12541 tcctatatga ttgaggagtg tgatatgaaa aaaatctgtg ttttcttttt ggctctatct
    12601 tttttctatg gatttgcgga aaatgctgcg tcgggagatt ttaatctttc tgttgacctt
    12661 gcatattatc ccttaagtga aaaaatttcg ggggaaacgc atttttcacc tctatctgct
    12721 ccatatagcg gggtagaatt acggactgta gcttctttta catggaccat accaactcct
    12781 ttctctgaaa atccgcttgt cagcggaaat aatctaaaaa taaagacggc tttagagctt
    12841 agtcccgtat ctttcgtgcc tgagctctct ttttctttta ctcctattgc cttcctcatt
    12901 ttttctacag ggataaaagc aggaagtgga tggactttca ctcctttatc tgtcgatggt
    12961 atggctcagt atgataatgt ttcttcatcc tataatcctc tgtccccatt cagtagcttg
    13021 tattacaatt tttgggctga agctttattt caatttgatt tagcggcatt gtaccctggt
    13081 gaatggaatc atattgccct tgttgcaaca tataagccat actacgaggc tctttctaaa
    13141 ggcggcgaag gggggaatcc ttggtattgg caaggtagcg gtgaaaaagc gaatggctgg
    13201 aagtattact cgtcaattat ttttgcatac cagatgcccc ttttgttgca aacaattgcg
    13261 gtgcaaactg aatttgaagg tttttatgat gcttcaagtt ttgcatcggc gtatcaagcc
    13321 tgggatccat gttttgcaaa aatttcgatc agcccgctgg tcaatttaga gttttcaaag
    13381 tctcagaatc taataattct ttttgggttt tcaagccgaa gaagtttttc tgcacaaaca
    13441 tcccatttac aaaatgattt agaaaaaaca actattgggc gagaatggtt ttttaatcgt
    13501 attgccttaa gttattcaat acgattttaa aactgtacaa aaaaacatac ccatttattt
    13561 aatagctgtc tacttaaata gactgttgtt aactagatgt tatctattct attgtattat
    13621 aacgaattaa ctgtttctct atgttggcat gaaacttgct agtaattagt aaaaggatgt
    13681 caataatgga aaatgagtta attacaccgt atttatcaca attaaaaaaa tacccacttt
    13741 tatcggcgga tgaagaaaaa aaacttgcaa attctatcga aaatggtgat atgagggcac
    13801 gcaacctatt ggtacaatca aacctacgat tggtaatatc catcgcaaaa aaatatcttc
    13861 attacaaggt atcgcttagt gatttattac aagaaggcaa tattggcttg ctgatagcgg
    13921 ctacaaaatt tcgtagtagc tttaatacgc gtttttcaac ttatgcgtat ttgtggatac
    13981 agcaatatat gcttcggttc atacgaaata aaaatcctgc gattcaaatt ccccatcgaa
    14041 aagaagctga aattcgtaga atacgggcga gtcaagatca tttacaccag catttaggga
    14101 gaaaaccaaa tgcagatgaa ctatctcttt acttaggttt aagcgaaaag gaaataaaaa
    14161 atattttaga tcatgagttt tcccttaaaa gtttagattt gattgtagat ataacaagtg
    14221 aaactacttt tggtcaattt ttaacagctg atacaccttc gccagaagaa gattttcttg
    14281 ttacagaaaa gcgtgaaatt gtcttaaata ttatgcaaga gcttccaaag aaagaaagag
    14341 atgttattgc tagtcgctat aaccttgcgc atattgattc aaaaaacacc ttgcgttttg
    14401 ttggctcaaa actagggctt tctacagaaa cggtgcgcca gatagaaaaa agggctttga
    14461 aaaagatacg ctctaaggct ctggaacgag gtttcgcgct ttcttgacaa tccaaaacga
    14521 aaggactagt ataaggcatt tatgaatgcc gaaaataaaa gaaaaaaaat aaaacgaaaa
    14581 aaaaaatcat ctccaaggcg aaccttgttg ttttttgcct ttgtgctgtt ttcttgttta
    14641 ttgctcattg ttttctcctc aatttttatt tcaaaagatg tagagcaatt tagtgataaa
    14701 caagaagata cgatgcttta tgaagaaaaa aaagctataa cagaggtttt gccggatatg
    14761 caagaaagct ctgtccctca aaaagagtat gtagaaaaaa ctctaacttc ttcctccaat
    14821 aaaacgcaat cctctgtccc caatgccccc aaagcggatc ttgaagttca aaaaggggcg
    14881 cttgtaaaat cctctgtccc tcaagtcgaa aaaaacatag atcgctctca ggttttggct
    14941 gaatcctctg tccctcaaaa aaaacgaggg agtatcattc ttgttcttga tgatggtggg
    15001 cataatataa gtcaattaca accctttttg aatttgcctt ttccgctgac tattgnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncataa tataagtcaa ttacaaccct
    15181 ttttgaattt gccttttccg ctgactattg ctattttacc tgctttagcg tatacgaaag
    15241 aaagcgcttc tttaacaaga aaggctggaa aagaagtgat gcttcatcag cccatgcaag
    15301 ctatcaattt atctgtggac ccaggtccaa gtgcaattct tccgaatatg actgaagctg
    15361 aaatacgtgc gattttacaa aaaaatgttt ccgagatagc tcccattgta ggaatgaaca
    15421 atcacgaggg ctctctcatc acagccgatg cgaatgcgat gtcagttgtg ctcgattttt
    15481 gtcgagatac agctttatta tttcttgatt ctcgcacgaa tgtcgaaaca aaggtttctt
    15541 ttgtagcgaa agagaaaaaa atgcagattt gggagcgaaa tattttttta gataacaccc
    15601 cggatagaga gaatatcacg tcgatgtttc agtcgggtct cgaaattgca gagaaaaacg
    15661 gctatgttat tatgataggt catgtatggt cgggctctca tcttgcacaa attttggatg
    15721 agcttggaaa aaaagcaata caggatggtt ttgtttttac aacactctct gcgttggaat
    15781 taaatcaata aaggtaatta aaaaatgaaa atacttggca tcgaatcttc ttgcgatgaa
    15841 accgcttgtg caatcgttga ggatgggaaa aaaatactaa gcaatgttgt tgcgacacag
    15901 attccctttc acgaggaata catgggagtt gttccagaaa ttgcaagtcg aaagcatatt
    15961 gagtggattc ttcctgttgt aaaaaaagcg cttcaagatg cttctctcaa tataagcgaa
    16021 attgatggct ttgctgttgc gaatcgacct gggcttatgg ggtctcttct cgttggttta
    16081 agctttataa aaacgcttgc gtggtctcaa aataagcctt ttatagcggt aaatcatatg
    16141 ctcgcgcatc tttatgcctc gcatttggcg gtagatattt cctaccccta ccttggcttg
    16201 cttgtttctg ggggacatag cattatttgc aaggtcttgg attttgatac aatcgaggtg
    16261 ttaggaacga caatcgatga tgctgtggga gaagcctttg ataaggttgc aaagttttat
    16321 aactttggct atccaggggg cgtcgcaatt gataggcttg caaaagaagg aaatccaagg
    16381 gccgcatctt ttcccatacc tgttttaaat aagggagaaa aaaaagagca ttggtacgat
    16441 gtctcgtatt cgggcttaaa aactgctgcg ataaatcaaa ttgatcagtt ttggcatgaa
    16501 ggctatgaaa aaacacctga aaatattgcc gcggcctttc aagaaacagc ggtgaatatt
    16561 ttattgacaa ggctttttcg agctgtagaa gatacgggaa taaaaacgat tgttgccggg
    16621 ggaggagttg ctgcgaattc tcggcttcgt gcattattgg ctgaaagaga cgatttgcat
    16681 tgtatttttc cgcctttaaa actctgcact gacaatggtg ctatgattgc ggggcttggc
    16741 tatcattatc taaaacgggg agaatcgagc ccactgacga caactgcaag ccctcgtgtt
    16801 actgagttca aaagaggact aaaggtgcag agggacagag cataattttt tttgagctct
    16861 tggggacaga ggatttctta aaattcgtta aaaaaataca attttcttgt aaaaaacgtt
    16921 tattaaaagc tcattcttga tgatttccaa agtgcacttg ttttttatcg ctttggggac
    16981 agaggattta aaagtgtaac aaaaaaaaga gttatacgtt ttctaatagc tcgctgtaat
    17041 agcgtgtttc aatgttttct tcaggaacgc ctgctttttc gagagttcga agtagttttt
    17101 tgtgtgcttc tatcgttctt tctgtatcag cggtttcaga aagtatttct atttcaataa
    17161 aatcgccgag tttatcaaca taggagactt ctatgagcgt gtcttcaaaa ttccatcgtt
    17221 ttgtgtcttt atgtttaacc atactcggag aaaatcccgc tgaggataaa aaagtttcaa
    17281 atgagagttt ttctttttct gcctcttttt tatctaagcc ggctccaatc gtaaattcgt
    17341 attcgtcatt gacttctatt tgtcctgagc gaatttcttt tttcttatag ttgaccctta
    17401 atggtttgtt cgtttcttct cgtaggcgca ctttttgagg atgtcctacg cgagattgcg
    17461 tccagtagac atcgcttttt tctgtttttc cgagatattc tgcaaattgg cttattttct
    17521 cttctacttc ttttctattt gtgaggctcg cttttaattc aatttcaaac ataataattc
    17581 caattatttg tatcctcgac ggatccgtat tgaccacctt ttaaaatttt ttgtatctgt
    17641 gtagcaactt ctccgggttc gcgattattg aatactgctt ctctaccttc atgcgtgatg
    17701 ctttcaatcg gcgttaagcc agctgcggtt cctgtaacaa agcattctac ggcattcttc
    17761 attacttcat cgatggtaac gagtcgctct tcaactttga taccgagttg gtttgccagt
    17821 gttatcgtgc tgtttcgagt tattcccgga agaatagtgt cgccgagttc aggggtaatg
    17881 agtgttccat cttttagctt aaagaaaata ttacatgagg agccttcttg gacgtatttt
    17941 ctgcttgtgt caaggaaaat aacttccata tagcctgcgt cttctgcctc ccgttttgca
    18001 agggcagata taacatagtt tgaagcacat tttatccagc ctgttccgtg ttcggttgca
    18061 cgaatacgct ctgttgtaat agcctttgtg ttgccgcctt taaaatatga tgagactgtg
    18121 gttccacaaa taataacata gggctcaact gatatgccaa cgcctatagc tccttctgta
    18181 ttcataaaag gtcgcatgta aacggcgctt gcacttgcat atgaatcttt ttcccattca
    18241 gcgttgtagc tcggaacata gccgaggact gcatttcgct ttacaaattc ttttgatgca
    18301 ttgatataaa ggtcttcggg aaaagcgggt gcttttaagc cacgcattga gtttgcgaaa
    18361 cgttttgcat tttggtcagg tcgaaaaatg gcgactcttc cgtcttttgt gggaaaggct
    18421 ttcatacctt caaaacagcc aaagccgtat tggctcgtat aactgacgcc agggagcttt
    18481 aagtcatttc gggcagcaag taccgcctct tttttttcgg gggacagagc atttagctca
    18541 tcaaaagtga tagtgtcaga ttcaatccac cgttcttccc atttttctgt ttcactatta
    18601 tattttgcca tgtaagtaat tgggtacatt tgcaagctaa aggccatttt atcctccaaa
    18661 ataaaaaaat atgcaattaa ttgtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18781 nnnnattatt aaaataagaa atacatggag gttggaatga aaaaagggat gaaggttttc
    18841 cttattgttt tggcggttgt tcttttactc attttttcgc tgtatcgatt ttgggcgggg
    18901 aattataatg caatggtaca aaagggcgaa acggtcgatg ctgcgtgggc acaggtacaa
    18961 aaccaatatc aacggcgttt tgatttaata ccaaatctag tgaatacggt aaaaggctat
    19021 gcgtctcatg aatctgaggt gtttacaaat attgctgatg cacgttctag ggctggtggt
    19081 gttttacagg tttctgatga ggtgctcaac aatcctgaat cttttgcacg ttttcaacaa
    19141 gcacaaaatg agcttggttc agccttacaa aggcttttag ttattaccga gaattatcct
    19201 gaattaaagg ctgaccaaaa cttcttagcc ttacaagacc agcttgaagg gacagagaat
    19261 cgcataggga cagagcgtaa gcgttttaac gatgtcgtgc gagattacaa tacctttatt
    19321 cgccaatttc ctcgcgtcat tattgcaaac atgagtgggt ttaccgcaaa acaatatttt
    19381 gctgcaggga cagaggcaca atctgctcca caagtgcagt tttaagtttt aggcttgcaa
    19441 gtttcaaaat tcacctaatg ttctaaggag taggaagtgc tttcaaaaaa aacaaagaag
    19501 atgataaaaa aactaaatct ttcaaaaaaa gattttgacg atatacaggc tgcagttgaa
    19561 aaagaagaaa aaaagacttc tggtgaaatt gctctcgctc ttattcgcga aagtgatagc
    19621 tattcttttt gggagctttt tttctctgtc attgtaggag gctttgtttt ttctctgctt
    19681 cttcctctgt ccccattctt tgagaaattt ttaacttctt ttctttggac ctattcttct
    19741 tggcaacttc ctgccataat cgggcttatc acattttttg ttattgccct tttatttaat
    19801 atcgcaaata ttccctctat cgatcgcttt attattccct atgtggttcg tcatcgagct
    19861 gtgtacctgc gtgcgcttcg ccattttgta gaaagtggag tctacgcgac gcgagatcat
    19921 tctgggattc ttatttttat ttcggtaatg gagcgcgaag tgcgtatcct tgctgatatt
    19981 gggcttgcag agaagattga gcaagaaaaa tggaatacta tcgcacaaga actctcagcg
    20041 gcttttaaag cgaatgcggt aaaagaaggt ttagaaaagg cgattcacga ttgcggactg
    20101 cttttgcaag agcattttcc tgtgcaagaa gataatccaa atgaactagc tgatggctta
    20161 gttgttttgg aggtagctga atgatacggg tgaataacgc aggttttcgt aaacattctt
    20221 ttatagtttt attttttgta ttagctgtct ctgttttttc gctttctgtt cctccgatgc
    20281 agggcgccgt caatgactgg gcaggtattc tttctgcgag tgaaaaaaat gagcttgaaa
    20341 catttttaaa tgcaacgaat gaccaaacat cagtgcaagt tgctgtttta acacttcctt
    20401 cactcgaggg agaatcccta gaggctttta gcatgaaggt ttctgaagct tggaagcttg
    20461 gacaaaaaga aaaagacaat ggtgttttgc tcctcgtctc catggaagaa aaaaaaatcc
    20521 gcattgaagt tggctatggc ttagaagatc gtctcacaga tataaagtca ggtttgataa
    20581 tacgaaatat tatagcacct gcatttcaaa gtgggcaatt tggtaaagga atcgtagaag
    20641 cagcaaaaac aattgtcggt atcgcaaccg ataatgaaga gattatttca aaaaaaatta
    20701 ataaaaccga tagcgacgac agtatcgttg gttttattat ttttattatc tttgttttat
    20761 ttatgactgg tgcccttggt cgaaggggac gcggaggctg tggtggaggg cttggaaatc
    20821 ttttttggct gcatctttta tttggtggca gcggaagagg tggctcctcc tttggcggta
    20881 ggtcaggggg cagttttggg ggtggctctt ctcgtggtgg tttaggcggc ggttttcgag
    20941 gtggtggcgg tagtttcggg ggcggaggtg cctctggtgg ctggtaaaaa tacaaaggca
    21001 aagatgatta gtagttaaca aaaagtaaaa tctaagatac aattatctct taataaaaaa
    21061 acaattagaa tactcttttt gaaaatgtaa aggaattatc atggcaataa attgtggtat
    21121 tgtagggctt ccaaatgtgg gaaagtctac tattttttca gcattaacgt ctgctccagc
    21181 tgaggcagca aattatccct tttgtacaat caatcccaat atcggtatcg tagacctacc
    21241 tgatgcacgg cttgattttt tagttaaaac gcttaaaccc aaaaacaagg taccaactac
    21301 ggtagaattt gttgatattg caggtttagt aaaaggcgca tcaaagggag agggtttagg
    21361 aaatcaattt ttggcgcata ttcgtgaagt gggcatgata gctcaagttg tccgttgttt
    21421 taaaaatgat gatattattc acgtaaacaa taaaatagac cctgttgacg acatgcttac
    21481 tatcaatatg gagcttgctt tcgcagacct tgcgacgctt gaaaaagcac tggaaagagc
    21541 gcaaagggcg agtcgggtgc aagcaaaaga tgaacaaaaa aaagctctgt ccctcattgc
    21601 ggttttggaa aaaattaagc cgcttttgga aaatggtgaa tctgttcgaa aagctggttt
    21661 gactgatgaa gaaaaagcgc ttgtttacga atgtcatttt ataacattaa agccgcagct
    21721 ctatgtttgc aatgtcgacg aagatggcat gcaaaatggc aatgaatata tcgatgcggt
    21781 gcgggcgcaa gctgaacaag aaggggcgaa acttgtggtg ctttgtggaa aatttgaagc
    21841 tgagcttgca gatatggaca gcgaagaaga aaaagctgct tttttagctg agctcggttt
    21901 ggaagagtct gcgctttctg tgcttgcacg agaggcttat gacttacttg gtttacgaac
    21961 tttttttaca gcgggagaaa tagaatgtcg cgcttggact atccgcaagg gggacagagc
    22021 tccaaaggca gctggcgtta ttcacagtga ctttgaaaga ggttttatca aggcggaagt
    22081 ttactcgtat gacgacctcg tttcttatgg aagtgaagca gaaatcaagg caaagggacg
    22141 ctatcgagtt gaaggaaaag aatatattgt ccaagatggg gacattattt tttttaagtt
    22201 taatgtatag ataaaagggg tacaataggt acctcttttt ttttatttta aaaaaattaa
    22261 taaaaaaaac aaaaaagtcc cgagaaaaac caataataag acatgaagaa agatacaaaa
    22321 ctatttaaaa tgcttgcatt ttcttttgtc cttgttttat tttttgcaag ctgtcacaat
    22381 ctaagttcct cctttgcagg aagaggctat cgtcaaggct ctctatctat tacatcatgg
    22441 aatattcaaa ccttctttga cacccaaacg agtggaaggg aatactcgga ttttatcggg
    22501 acaaaacttt ggaacgatgc tgtgtatgta aagcgtcttg aaaggctttg tcaaacaatg
    22561 gaagctttaa gttctgacct ctttattttt caagaaattg aaaatgaggc tgtgctcgta
    22621 gatattgtaa actacttaaa gactcaggga aaccccgcat tagcatactc gcatgctgtt
    22681 tttatacctt cgagggatga tgtgctcggt tgtgctatcc tctcgcgcta tccaattatt
    22741 tcttgtacga cgcatcaaat tgattatcgg tttgagggga aaaaacagcc ggatactcgg
    22801 cctttgatgg aagttttagt acaagctcat ttttcaaagc cgcttcgagt gtatacgagc
    22861 cattggaaga gtaaatcagg tggagctgag cagtcagagt tctggagaga tgcacaggaa
    22921 gctttaattg cgcggcgaat acgagaaaca ggtgatcaac tctatgtctt tgcagcagat
    22981 tgtaaccgtg atttatcaga atttgcgcaa gatggccact atattgtttt ggatactgag
    23041 gggcaaaggc ttaaaacaca atcgccctat ttatttgacg cttttgctga tgaggacggg
    23101 acctattttt tccaaaaaga atggaacaaa atagatcatt tttttatcgg agaagccttg
    23161 catattcttt cgttttgtat tgagcgtaag gggcttcatg ttgataaaga cggtattcca
    23221 tatcgttttt ctatttggaa tgggcagggt tattcagatc atcttcctat atcctgtgta
    23281 gtgagtgaaa aaatgaataa aaacgattaa aagggtaact ttctcacggt ctttcgtgta
    23341 taataattgt atattaagta tatacatgat tgaatcagag gagaaaacta tggcaactat
    23401 tacattcaca aaagacaatt ttgaagcaga agtactaa
//
LOCUS       DAOSSW010000019        22960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_2419_length_22960_cov_6.828596, whole genome shotgun sequence.
ACCESSION   DAOSSW010000019 DAOSSW010000000
VERSION     DAOSSW010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 22960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22960
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2419_length_22960_cov_6.828596"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..470)
                     /gene="ssrA"
                     /locus_tag="PKW26_03010"
     tmRNA           complement(117..470)
                     /gene="ssrA"
                     /locus_tag="PKW26_03010"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(532..2034)
                     /locus_tag="PKW26_03015"
     CDS             complement(532..2034)
                     /locus_tag="PKW26_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92571.1"
                     /translation="MKRYLVSIFALFFCFSLSAKELNVELLHVDTVLVYREDGKADIS
                     VKTRWNVSSGAMSGFYFSGEKNAIQFDLHKSWAEGFEHNTRQEFPLDILPLAEGGYDI
                     IIADAKSLSGLVDFVFTYRVNLAASGHTSLVDEKDRLSDKNNRLFYFNWAPVDWDLTA
                     KSRTTEIIFPISIDDLIITDSETKQRKTELFLQTLGLNISKETIASNTKIDWYEVEGL
                     DKKSYLSLRFYQENLREKESQPIILYLDAKSDLLVDNPMIGQNILSDTGDSFSFQNWE
                     DKNLAKKNTRAMKILYVLLFLFFCFSSFSFIIVFLKQKKKVKSEKKEVLKMLFPNVET
                     VAVRKNIHPIEIAFLLAFPLVDFIYLVLESLFLAGKIEYKNSKKLRIEKKNLVVFDTF
                     ENDFLSCFDDEGFLVLFKIERFYTKGIKKTKTDTTYDLTETIAYWSEKITSIQFDSLE
                     DKPFVTYWWTFSIYAKYMQNTFEATQSLFISPVYYQIVAAYEMEKKSPYF"
     gene            complement(2057..2353)
                     /locus_tag="PKW26_03020"
     CDS             complement(2057..2353)
                     /locus_tag="PKW26_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92572.1"
                     /translation="MNFAQEILIIIKLVFGGLASFFAILLWSKTRDLAWMSLVIASLC
                     TYAAIVYDVLENLGVIPIGLYTFYGISIASLFFAIVPSIFYIISFAIMLKRLSK"
     gene            complement(2431..3219)
                     /locus_tag="PKW26_03025"
     CDS             complement(2431..3219)
                     /locus_tag="PKW26_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92573.1"
                     /translation="MDTIHSDEYQKCFKKKYGFVKRARGSFLYTEKNIRLVDLAQNEG
                     RAILGWRHGSSMLSFKNTLEKGLWSSYPNGAEHRLEKALSSLFSLCGYAEYTSFYWYI
                     GKPDKDFSLKNKQSLYPWSGLYPLSDDNAGTRIKDETLLFIPPLPWPSLYILAGKNSS
                     LPPSDRLSTCLIEALTRSLYDLIKEFPKRPPESWGKFDSILNAYFIREGSLLKPKLKG
                     KDYDAFALHCLESEIYIAERNPEKEEAPPSFVPWFANPSAFKKL"
     gene            complement(3261..5528)
                     /locus_tag="PKW26_03030"
     CDS             complement(3261..5528)
                     /locus_tag="PKW26_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOQ92574.1"
                     /translation="MGIMEYLQSLNEEQLAAVEHSGSPLLILAGAGSGKTRVITTKIA
                     YLISEKKIKASSILAVTFTKKAAAEMMLRARELEPLASHSQIRTFHSFGAWFLRLHHQ
                     EAGVDPNFTVYDDDDSAQLLKKAVPSLSTQDAKYMAHKIARAKDYCYGPESEYLCEIE
                     EDLEFPRYYAAYERRLRETGNVDFGDLIQLPVMLLQNNEGIRNFIHRLFKVVMVDEYQ
                     DSNVAQFQLLQQLVGPETYVCVVGDDDQSIYKFRGAEVQNILNFQKEFPGTELIKLEQ
                     NYRSRSGILKLADGAIKNNEDRLGKTLRAVRGMGKRPVVVFLATQDDEVRYCAKLITE
                     AKHKGVPYNNWAILYRTNAQSMGFETEFLKQKIPYNVVGSLKFYEREEVKDILSYLSF
                     LVNPRDEIAFRRVVNKPARAIGSITQDKIINLSKERLLGTEAIHQNIYEACQQFLTQA
                     SKKAKEGLETFLSIIETGKNLLEESIDEKQTLSVLIEKIITISGLDAYHAAQDEISGS
                     TKLANLQELINSALPYQKTTEGLVAFLEHIDLDRALMQELQEGEDRDAVTLITLHNTK
                     GLEFERVIITGMEEGLFPRSDKTDEELEEERRLFYVGITRAKDELYFTNCVVRRVWGY
                     TQYLPVSPLLLEIAHLDLEIQGKPPHLFSLARNTAFNASKKMLSKNSSSDESFDNFFL
                     EEEQKTVLAEDKKTNSNDWSRGQKIFHDDFGYGAIFDVKETSGEITISIQFENGQIKK
                     ILPKYAGHKIMAVKD"
     gene            complement(5596..7032)
                     /gene="rimO"
                     /locus_tag="PKW26_03035"
     CDS             complement(5596..7032)
                     /gene="rimO"
                     /locus_tag="PKW26_03035"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOQ92575.1"
                     /translation="MTKPKATQKLFFLDQYGCAKNQVDGEVLTAFLSEDGWLRCENST
                     EASLIIINSCGFIESAKKESIEALLHAREIHPDKKIILAGCLSERYAKELSEEMSEAD
                     FFMGNGDLSEIKKIVKKLFPEFDAPSYDEFRFSTPPQEGVSTTKRNDLFNFPGSAYVK
                     ITEGCNNCCSFCAIPLIRGNLRSRSIEDIKAEVIDLLEKNVFEINLIGQDLASYGKGF
                     IDIENPNAVFSPLHNLLKALLEIQKPFWLRLLYIHPDNFPLDILPLFENEKRLLPYFD
                     IPFQSGDRDILIRMNRRGNTSDYLLLIEKLRLSGSRSPYADSSIRTTFLTAFPGESDE
                     AHKNSKNFLEAIQPDWSGSFVYSKEEGTKAYELGKTIVAKKAEKRQKELQNIQAHITR
                     ARLKRFVHQKISVLVEELIIDPEKTEGLAIGRAWFQAPEVDGSIVIRYDLDDAGAIDA
                     VKPGNLVEVKITSSSDLDLDSVFIAPTQ"
     gene            complement(7025..8107)
                     /locus_tag="PKW26_03040"
     CDS             complement(7025..8107)
                     /locus_tag="PKW26_03040"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ92576.1"
                     /translation="MESFGEILKIAREKREIDLEKVSHDTSISIEYIRALEMEQTDVF
                     PGEPYLVGFLRNYAEYLNLDAKYLIQLYRSKKIQEAPIPPGLLRDEKTFPWKIVLPLG
                     IALSILVGLGLFFFIRHINNDSGVELLEKTDEVQIFTLTSVPIQKRVYENDIISVMLA
                     SGQVELLVEKTKEQLVLGTPNGKQQVQLGEEVKLDIDSVQGPDIVIFVSDIAKNDASR
                     GAEIRMFALSETEMREQPLSLDGIPLITEVDKNLSQSIIFDGTRAYPFTVNITFRGSC
                     LFRSQADNKDIIEDYFSAGDLQTITAQNGVRLWMSNANALKIQVIGDGKTHDLDVGRP
                     GQVLVQDIKWVKDIDGRFKLMVLLVD"
     gene            complement(8121..8828)
                     /locus_tag="PKW26_03045"
     CDS             complement(8121..8828)
                     /locus_tag="PKW26_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOQ92577.1"
                     /translation="MRKKTLFYISVVLMACFSLNSLLAQEIQTAQNFFQSISEYYANI
                     TDYEADLEIRAGSQNMSAKVSFKKPNLLRIDFSRPDTQVILFNGSLLTIYLPGSSAVL
                     TQSVSKDSGAAGGANLATPQGLNLMSRYYIISYAEKNTPVTLDDLEKNPNGSSEMVMK
                     LLLSRRNASEGFRTIVLSINPTTKLIRRVEATTPANESFVFTFYNYAINQKIPDTRFI
                     YDSPGTANTYNNFLFTE"
     gene            complement(8887..10191)
                     /locus_tag="PKW26_03050"
     CDS             complement(8887..10191)
                     /locus_tag="PKW26_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92578.1"
                     /translation="MEEKELRNLEELLESHDLENLSSKKILSKKPLSKAMQKYLEKKL
                     ELSLLNCGEKIPMHPIALCIPVSYEYPHFFRTLRSLHLSAMKALSYLGEKHQAFSIVC
                     CINARENDALEVKENNAKLLHELRRLQKTEFDGPFMNLHILDYSHEGAYFKKKDGVGL
                     ARKIALDWALKSGAEVLASLDADCLVSENYLYTLALFYQKYKNKSEWALCGFSHQEPA
                     EKAHKKAMDAYETYLHEHSRFLFDIESPYYPVALGPTIICTSEAYVRSGGMNKKIAGE
                     DFYFLQSLIKLNLKKRIEDYLFLASKVYPSARLSKRVLFGTGTALEEILGGKKKGFEK
                     KQYIAAKDFFSLFNTMLLSFYEREEKSIEMLLEFEKKVKNSSSQVYAFLAEDRFWIDW
                     EKIVKTHFSSKENLRKAFFERFDGLKTIRLFNYLAEVHKSDF"
     gene            complement(10194..11201)
                     /gene="miaA"
                     /locus_tag="PKW26_03055"
     CDS             complement(10194..11201)
                     /gene="miaA"
                     /locus_tag="PKW26_03055"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOQ92579.1"
                     /translation="MQKKYSFNTEKYNALVLLGPTAVGKTPLANRLAEYFSTELISAD
                     SRQVYKGLDIGSGKDLDEITVPYHLIDIADLRDEYSVYDYQKDFFSVFNGLAKKTIQP
                     VIVGGTGMYLDAIIRDYSLIKVPTNISLRESLNGKTMDELEAILREMKPDLHNKTDLE
                     ERHRLLRAIEIELYYKEQGLDAEKKQSIKAGDVKPQSDESKAEFKALVLGTSFPREVL
                     RKNITERLKKRLESGMIEEVENLLSSGISHERLQRLGLEYKFLSLYLLGGLTKDALFS
                     KLNTAIHQFAKRQETWFRGMERKGVAIHWIPCDGSETEVSIEGRFAKAIDILYREKYL
                     V"
     gene            complement(11202..11978)
                     /gene="dapB"
                     /locus_tag="PKW26_03060"
     CDS             complement(11202..11978)
                     /gene="dapB"
                     /locus_tag="PKW26_03060"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOQ92580.1"
                     /translation="MKIALLGYGKMGHMIEVEAKKKGFEISATIDSYAKDASHFISSP
                     ADIVAALQETKSEVVIDFTHPDAAFENIKAIIPLGIPLVVGTTGWLSHLSEVQDLVAK
                     HSSSLFYAANFSIGVNLFYKVVAEAAKVFSSYDDYDVALWEAHHNQKADSPSGTALEL
                     AKLVLENHSGKTEMHFDAFHEKPKAHQLHVSSTRCGSVPGTHTVFFDSNADSIELLHR
                     ARSREGFALGAVHAASWLFDGLKKGSVQKGRVYTMEDMLS"
     gene            complement(11994..13334)
                     /locus_tag="PKW26_03065"
     CDS             complement(11994..13334)
                     /locus_tag="PKW26_03065"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOQ92581.1"
                     /translation="MIVMKFGGSSLATAERIQHVASIIEAYKEKKPLVVLSAMGDTTD
                     FLLEASDLAVEGRVDISSVEKLHIDTAKELAIDAREIEALLEELKTLLTGISMLKELT
                     KRTRDYLVSFGERLSVRMMALYLDKKGSAARFYDAWDAGFISDSNFMSAELKDSVWTT
                     IPKAFEDYTAGRQKEIPIVTGFIAKDINGYITTLGRGGSDLTATMLGAALNVEEVQTW
                     KDVDGILTTDPRIVKDARPIEEVTYEEAAELAYFGSQVLHPRSMQPCRKTGTPVRVKN
                     SYNIASPGSIIVEKHKGEVSPVRAITAVKGVTIIDLVSTRMLGASGFLAHIFNNFLKW
                     DISIDVIATSEVSVSLTVNTKQHLEPLIADLQNVADVELKTKKAIVTVICDVKRSSSI
                     LSACFKALQDNAINVQMISQGASKVNVSMLVEETEVNEVVQVLHKSFIDNKLIY"
     gene            13380..15281
                     /locus_tag="PKW26_03070"
     CDS             13380..15281
                     /locus_tag="PKW26_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOQ92582.1"
                     /translation="MFRKGNLIIYKQKPAVIQAIEDKISISFVSGFKGKKALIENQKV
                     RDKDILFLHPGPAENLTSLVTEDNIKASDTFTKAIIETWELLAEEEQAFRFDEIAELS
                     IDTKVLENIFPLYFSLIDSVYFKTEQKESQLFFIPRSKDEVAALLEKENEKQNEAAIK
                     DAFIKRLRQKKADLESDAKFLQEIEAFALGKTDKPKYLKEAHVKETIEEAHKLLLDVG
                     FWTMSKNPYPARHGLSMHSSTSFLDSPPHEDDRIKLEHLAYAIDNEGSTDPDDAIAYD
                     GEHLWIHIADPASIVLPDSAIDKAARNRGATLYIPEGASRMLSEKSLKDYALGLEGEN
                     SYSRALSFKIRITEDAVVEDVSILKSFVKVKRLSYKEADEQKNSSQLAPLFRLADKNF
                     TKRKNAGAIQIQFPEASVILTKNEGADPSVEIAPLVEYESFALVREMMLLAGEAAARF
                     AFKNNIPFPYVSQEEPEIPKKLPEGLAGQYRLRKSMKARSLGVTPSQHASLGLSFYSQ
                     VSSPLRRYSDLVAHQQIRLFLDNKTVLTGDAVLERISQGDAASRAIMRASRESELHWK
                     LVYLLQNSEWEGRGIILEQKGKTAIVFIESLAMEASVHAPKAELNDKIFLRVGKIQLT
                     KLEVQFIEA"
     gene            complement(15351..16463)
                     /gene="ugpC"
                     /locus_tag="PKW26_03075"
     CDS             complement(15351..16463)
                     /gene="ugpC"
                     /locus_tag="PKW26_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOQ92583.1"
                     /translation="MAKVELKGIGKVYDNVFRAVDNANIVVDDQEFVVFVGPSGCGKS
                     TTLRMVAGLEDITEGELYIDGELMNDVPPKDRNIAMVFQNYALYPHMTVYDNMAFGLK
                     IRKTDKAEIERRVNEAARILDIEKLLDRKPKALSGGQRQRVAVGRAIVRNPKVFLFDE
                     PLSNLDAKLRVQMRAEISDLHHRLKATMIYVTHDQIEAMTMSDKIVVMKDGKIQQIGS
                     PLYLYNHPINKFVAGFIGSPPMNFLTVKIIEKGGKLIADEGSFEIIPTDEQAKTLKNY
                     IGKEVFFGFRPEDLTYVKTPAPVNNMQMKISVIEPLGAETHLHLQTKTQQVIAVVEPD
                     NKYQIGDTANFTPLLEKGKFFDKDTELNICEVVKSE"
     gene            complement(16610..17773)
                     /locus_tag="PKW26_03080"
     CDS             complement(16610..17773)
                     /locus_tag="PKW26_03080"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ92584.1"
                     /translation="MKMSAPIFLLFAIYGVINVYLPLVLRSMGFTPSHVGYLLALFEV
                     AGLVFPFLLSPFISKKGLHGPFLFISGIILSLLPFPLVKIGGFTMTAILLAMYATGYK
                     GIVPVSDSMTNLLLGDEHTKYGRVRVMGSIGFVLMSLFLQFIVKPEQASQNTIIFWMC
                     IPSLLFALSILFIPGLIKRQPPDAEAHVETEFEEKKSFFNAFKNFPKVFWVGLFLIFC
                     GYLGMTPSQRFFSMYIYEYLGLQASGFLWALAAIAEIPFMFFANRFLRRYGSMRLLVF
                     GTFFVFVRIITYILIPNFTGALIAQLFNAFTYGLYHPAAIFFVAEHTPRKNLVVGMTL
                     YSIVAIGAGSIIGNLIGGLVIEYFGYPVLFTSFSLVPLLAVIIYFIFFMKEYR"
     gene            complement(17829..19877)
                     /locus_tag="PKW26_03085"
     CDS             complement(17829..19877)
                     /locus_tag="PKW26_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOQ92585.1"
                     /translation="MKKQRLWKLCEKTIPSFSTPLGCFYDEETHTAHFSIWAPTASKV
                     RVLLFDDGKTEHFSYNFPLIIDRKTGVWTLRCSVDVPLDTMFYEYEITTEKGSLSCLD
                     PYALSMAAFTNDNTSGRAAIINPNSPAYLPKGGWSKENSYEENLSALAAFNHYTDAII
                     YEVSVRDFTISPDARLDKANLLGHPGSYNAFVQKLPYLKKMGITHIQLLPVLNFYNND
                     ETKLDFEKSTALYNNNYNWGYDPHNYFTPEGWYASNPHDPYCRISELKTLIKEAHLQG
                     LRVILDVVYNHMAKIDFLDDIVPHYYFRLSKQDGSFLNNSACGNDIASERIMARKLIH
                     DSLVYLVKEYHVDGFRFDLMGLMDSQTVLDAYDACVLYNKDILFIGEGWKMYNGPKGT
                     QGMDQNFMTKTQKVSVFNDEFRDLAKGGGTNEDLKGFLTGGSIFTKDLFYNVCALPQV
                     NFKVNSPARSVQYFECHDGLTLHDVIAHNMNFDLSNSMHLDELYARLRLAHVLLFTSQ
                     GIPFIQAGQENARTKPKASVRDFDSSDIHGNFVRNSYKASDTINSFSWTLDAQREKLN
                     AFVRSLIALRKKMPHFRLHTKELVESSVHFLQTQSSDLLLVYTINLKHQGFDEVCYLI
                     FNARKTISSVELNIDFSQAKVYVHGERVSVDGFSFDSSEYGGKTIDIAPLSALVFFVP
                     "
     gene            19959..21125
                     /locus_tag="PKW26_03090"
     CDS             19959..21125
                     /locus_tag="PKW26_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOQ92586.1"
                     /translation="MKKNLYFDCAATALYDEAIIKNALEKSLCFFANPSSLHKKGLEA
                     KQCLEACREKAAKSLEIPAEQLFFTSGGTEANQIAILSLLTRPSKGSILISAIEHPAV
                     REQVKMLESSGWSVQTIPVNTEGSINLRSLREKLSADTALVSVMAVNNETGAIQPIEK
                     IGALLQELYEGKRKPRFHVDAVQAIGKIPFSLKQTAIDSAALSAHKIGGPKGVGALYT
                     KHQMESFLKGGNQERGLRSGTENLFGIYAFADCLEKYTIGEKTQAFECYEKQKTLTAF
                     FIEEVKNIKAATIIPKTRTGKEGESFSPWIVQAAFQNIPAQVLIRALSEHGVCISSGS
                     ACSSKKLSRPVLEAMNLPKEQIQNAVRFSFCPSHTREDIESLLALLKNTVNQYG"
     gene            complement(21109..21876)
                     /locus_tag="PKW26_03095"
     CDS             complement(21109..21876)
                     /locus_tag="PKW26_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ92587.1"
                     /translation="MPSSNNTQEKALSLKIEKVSLTRNDKLLFRDFSHVFDASLIHAI
                     LGPSGCGKTSFLHAIAGLLPIDDGKISKITALSYLFQEPRLLPWLSLEKNLSLVIEKL
                     YTDKNLAKEKVASMLEKVHLIDYAKRFPSEISGGERQRLALARAFLFPSPLLLMDEAF
                     QSQDIVLKLQLMDLLQSLHEEDRRTIILVSHDIREALSLADTIMVLKGSPIKKELLHS
                     MTQEKSQKQSASKTYVNFPQDLQERENDILRVFSHID"
     gene            complement(21869..22621)
                     /locus_tag="PKW26_03100"
     CDS             complement(21869..22621)
                     /locus_tag="PKW26_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOQ92588.1"
                     /translation="MKKILFVFFIPTAILLCVWYLASIHIASSLILPSPLFVFTRFLL
                     LIKENLFWEHVFYTILRTILSFLYSLVISIILGIAIGFSKSFSLLLKMPLSVIKATPV
                     VSFILLALFWFTTSQVPIFVSVLMTLPIITSSVALGIANIDIKLRQMARVYGLTKKQK
                     ILHIYLPSVFPSFASGALSAFGLSWKVVVAAEVISLPKRAIGTSLQTAKVHIETADVF
                     AISLLIIALSFVLELLLSALFKNCMKWREKDA"
     gene            complement(22648..>22960)
                     /locus_tag="PKW26_03105"
     CDS             complement(22648..>22960)
                     /locus_tag="PKW26_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ92589.1"
                     /translation="MTVLVGRKSFVEENKDLVKKVLKEVAAAIDYTNKNPEKAGQLVE
                     KYSLGLKAPIVTKSIPTSAFAYSSAVDAKKDIEDLLSVFLDFAPEAIGGKLPDDSFYF
                     N"
BASE COUNT         7211 a         4521 c         4289 g         6939 t
ORIGIN      
        1 tccttttttc gtccatgaaa aaaggaacta tctcgttttg gtgaagtaat ttatatagaa
       61 aaccaaaacg agtgagctta gtccctgcga catccatgtc gcaaaaacaa taagaacgtt
      121 ggagatgggg ggaattgaac ccccgtccga atacactgac caggaacgtc tacaggttta
      181 tcggtgcgag gtagttgtcg agaggcggtg tcagcaccga aagcgtcgcc tccgtattct
      241 agaaaatgtc ctctctaaag cctagactct ttagaaagca agccctggtt tgtgacagaa
      301 agcaagaccg ttcagagctg caccgtcttg tttccgcgct aagctgaaat taagcagcta
      361 aagcgtaatt gtcgttattt tcggcaatta taagtttcgc ctgtttagag gaccggcaac
      421 ctcacctgca gttcaagacc acgaataccc gtcgaaaccg gaacaccccc gatttatagg
      481 ttttagtctt agactaaata aaatatacgc ttttttcttt tataagtcaa gctaaaaata
      541 tggactcttt ttttccattt catatgcggc tactatttga taataaactg gagatataaa
      601 aagagactgc gtagcctcaa aggtattttg catatacttt gcatagattg aaaaagtcca
      661 ccaataggta acaaagggtt tatcttccaa gctatcaaat tgaatgcttg tgatcttttc
      721 actccaatac gcaatagtct ccgtgaggtc ataagtcgta tcagttttag ttttttttat
      781 tcccttagtg taaaaccttt caattttaaa taaaactaaa aacccttcat catcaaagca
      841 tgataaaaaa tcattttcaa atgtatcgaa aacaacaaga ttttttttct ctatcctcag
      901 tttttttgaa tttttgtatt ctatttttcc agctaaaaaa agactttcca aaacaagata
      961 aataaaatct acaagtggaa aggccaataa aaaagcaatt tcaatgggat gtatattttt
     1021 tctaacagct actgtttcca catttgggaa aagcattttt aaaacttctt ttttttcact
     1081 tttcactttt ttcttttgtt tcaaaaaaac aattataaaa gagaacgagc taaagcaaaa
     1141 aaacaaaaac aacaatacat agagaatctt cattgctctt gtattttttt tggctaggtt
     1201 tttatcttcc cagttttgaa aagaaaagct atcgcctgta tcggataaaa tattttgccc
     1261 tatcatagga ttgtcaacaa gcaagtcaga ttttgcatct aaatacaaga taataggctg
     1321 gctttctttt tcgcgtagat tttcttggta aaagcgcaag gagagatagg attttttatc
     1381 caagccttca acttcatacc aatctatttt tgtattgctt gcaattgtct ctttactaat
     1441 atttaaacca agtgtttgta aaaagagttc tgtttttctt tgtttcgttt cgctatccgt
     1501 aattattaaa tcgtcaatag aaataggaaa aattatttct gttgtacgtg actttgcggt
     1561 taagtcccag tcaacaggtg cccaattaaa ataaaacaag cgattatttt tatcgcttag
     1621 tcgatctttt tcatcgacaa gagaagtatg ccctgaagca gcaagattta cacggtaggt
     1681 aaaaacaaaa tctacaagcc cagaaagaga ttttgcatct gcaataatta tatcatagcc
     1741 cccttcggcc aagggtagaa tatcgagagg gaattcttgc cttgtgttat gttcaaaacc
     1801 ttccgcccaa gatttatgga ggtcaaattg aatcgcattt ttttcgccag aaaaataaaa
     1861 acccgacatc gcgccactac ttacattcca tcttgttttt acgcttatat ccgcttttcc
     1921 atcttcgcgg tatacgagta cagtatctac atgaagcaat tctacattga gctcttttgc
     1981 actgagagaa aaacaaaaaa ataaggcaaa tatgctaact agatatcttt tcatttttta
     2041 agtaaaactt ccatttttat ttgctcaagc gtttaagcat tatcgcaaaa gaaataatat
     2101 aaaaaatcga tggtacaatt gcaaaaaata aagatgcaat agagatgccg taaaaagtgt
     2161 aaagcccgat aggaatgacg ccgaggtttt ctaagacatc gtagacaatc gcagcataag
     2221 tgcaaaggct tgcaatcaca agagacatcc aagcgaggtc gcgtgttttt gaccaaagca
     2281 ggattgcaaa aaatgaggca agtcccccaa aaacaagttt tataataatc aatatttctt
     2341 gggcaaaatt cacaacggct cctctttttt atgctatttc tagtatcgtt ttttctctat
     2401 acttttaaat atcgacaaaa aacacacaat ctaaagtttt ttgaaagcgg atggatttgc
     2461 aaaccacggg acaaatgacg gaggtgcttc ttctttttct ggatttcttt ctgcaatata
     2521 tatttcactt tcaaggcaat gcagggcaaa ggcatcgtag tcttttcctt ttagttttgg
     2581 cttcaacaag gacccctctc gaataaaata ggcattcaaa atggaatcaa attttcccca
     2641 agactcagga ggtctcttgg gaaattcttt tattaaatca tacaaggaac gagtgagtgc
     2701 ttcaataagg caggttgaaa gtctatctga tggtggaagg gaactatttt tacctgcaag
     2761 aatatacagc gagggccaag gaaggggcgg tataaaaaga agcgtttcgt cttttattct
     2821 tgtacctgca ttatcgtccg agagtgggta taaacccgac cagggataca aagactgctt
     2881 attttttaga ctaaaatctt tatctggctt tccaatatac caataaaaag aagtatactc
     2941 agcatagcca cagagagaaa aaagactcga caaggctttt tctaaacgat gttcagctcc
     3001 attcgggtag gaagaccaga gtcctttttc aagtgtattc ttaaaactaa gcatactcga
     3061 gccatgtctc catccaagaa ttgccctgcc ttcattttgt gctaaatcaa caaggcgaat
     3121 atttttttct gtatacaaaa aagatcctcg cgctcttttt acaaaaccgt attttttttt
     3181 gaagcatttt tgatattcat cgctatgtat tgtatccatg cctaatcctt ttgacagcgt
     3241 attttaccta ctgttttttc tcagtctttt accgccataa ttttatgtcc agcatacttt
     3301 gggagaattt tttttatctg tccattttca aactgtatag aaatagtgat ttctccactt
     3361 gtttctttta catcgaaaat agcgccgtag ccaaaatcat catgaaagat tttttgacca
     3421 cgactccagt catttgaatt cgttttttta tcttcggcta aaacagtttt ttgctcttct
     3481 tctaaaaaga agttatcaaa gctctcatca cttgaactat tttttgaaag catcttttta
     3541 cttgcattaa acgctgtgtt gcgcgcaagc gaaaaaaggt gcggcggctt tccttgtatt
     3601 tctaaatcga gatgggcaat ttcaagcaag agcggactta ccggcaaata ctgtgtatag
     3661 ccccaaacgc gacggacgac acagtttgta aaataaagct catcctttgc tcgtgtaatg
     3721 ccgacgtaaa aaagacgtcg ctcttcttcg agttcttcgt cggttttatc gcttcgcgga
     3781 aaaagccctt cttccatgcc agttataata actctttcaa actcaagtcc ctttgtattg
     3841 tgcagggtaa taagcgtaac cgcatcccta tcttcgccct cttgcaattc ttgcatcaag
     3901 gcgcgatcta aatctatgtg ttctaaaaaa gcaacaagcc cctctgtagt tttttgatag
     3961 ggcagcgctg aattgataag ctcttgtaag tttgcaagtt ttgtagagcc agagatttca
     4021 tcctgggcgg catgataggc gtccaaacca gaaatcgtaa ttattttttc gatgagaaca
     4081 gaaagagttt gtttttcgtc aatgctctct tctaaaagat tttttcctgt ttcaatgatt
     4141 gacaaaaatg tctctaaacc ctcttttgct ttttttgaag cttgtgtgag aaattgctga
     4201 caggcttcat aaatattttg atgaatagcc tctgtcccga gcaatctttc ttttgaaagg
     4261 tttataattt tatcttgggt gatagagccg attgctcgcg ctggtttatt gacaacccgc
     4321 cgaaaagcaa tttcatcgcg aggatttacc aaaaaagaaa gataacttaa aatatctttt
     4381 acttcttcac gttcgtaaaa ctttaatgag ccgacaacat tgtagggaat tttttgtttt
     4441 agaaattccg tttcaaagcc catagactgc gcatttgtcc gatacaaaat cgcccaatta
     4501 ttatatggta cgcccttgtg ttttgcctcc gtaatcagtt ttgcacagta acgcacttca
     4561 tcgtcctggg ttgcaagaaa aacaacaaca ggacgttttc ccatgccacg aacagcacgg
     4621 agcgttttgc caagcctatc ttcgttattt tttatcgcac catctgcaag tttcaaaatc
     4681 cctgaacgag atcgatagtt ttgctcaagt ttaataagct ctgttcctgg aaattctttt
     4741 tgaaagttga ggatattttg tacttcggcg ccacgaaatt tatatatcga ttggtcgtca
     4801 tcaccgacga cacagacata ggtctcaggt cctacaagtt gttgcaaaag ctgaaactgc
     4861 gctacattcg aatcttggta ttcgtcaacc atcaccactt taaaaagacg atgaataaaa
     4921 ttgcgaatcc cctcattgtt ttgtaaaagc attacgggca attgtatgag atcaccaaaa
     4981 tcaacatttc cggtttctcg aaggcgcctt tcataggctg cataataacg cggaaactct
     5041 aaatcttctt ctatttcgca gagatactca ctctctggcc catagcaata atccttcgca
     5101 cgcgcaattt tatgagccat gtattttgca tcttgtgtcg aaagagaagg aaccgctttt
     5161 tttagcagtt gcgctgaatc atcatcgtca tagacagtaa agtttggatc gacaccagcc
     5221 tcttggtggt gtaaacgtaa gaaccatgca ccaaaagagt gaaaggtgcg aatttggcta
     5281 tgcgaggcaa gcggttccag ttcacgagcc cgcagcatca tttcggctgc agccttcttc
     5341 gtaaaggtaa ccgccaaaat cgatgaggct tttatttttt tttcagatat gaggtaggca
     5401 atcttagtcg taataacgcg agtttttccg ctccccgccc cagctaagat aagcaaggga
     5461 gagccagaat gttctactgc tgctaattgc tcttcattta agctttgtaa atattccatt
     5521 atcccaataa tatcccttgt gtttattttt gtggcaatac aaacaaatta gaatcacatc
     5581 aaaaaaataa ttcttttact gagtaggtgc aataaaaaca gaatctaaat caagatcgct
     5641 tgaagaagta atttttacct caacgagatt tccgggtttt acagcatcta ttgctcctgc
     5701 atcatctaaa tcatagcgta tgacaatgga gccgtcaact tcaggagcct gaaaccaggc
     5761 tcttccaatt gcaagccctt cagttttttc tggatcgatt ataagctctt caacaagaac
     5821 agaaattttt tgatgaacaa aacgttttaa acgcgctcgt gtaatgtgcg cttgtatatt
     5881 ttgcaattct ttttgtcgct tttctgcctt cttggctaca attgtttttc ccagctcata
     5941 tgcctttgtg ccttcttctt tgctatagac aaaggagcct gaccaatctg gctgaattgc
     6001 ttctaaaaag tttttgctgt ttttatgagc ttcatcgctt tcaccgggaa aagcggttaa
     6061 aaaagtggta cgaatagaag aatctgcata aggacttcga gaacctgaaa gacgaagttt
     6121 ttctatcaat aaaagataat cacttgtgtt tcctctccga ttcatacgta taagaatatc
     6181 tcggtcgccg ctttgaaagg gaatatcaaa gtagggaagc aagcgttttt cattttcaaa
     6241 aagcggtaaa atatcgaggg ggaaattgtc gggatgaata tataaaagcc gtaaccaaaa
     6301 aggtttttga atttctaaaa gagccttgag taaattatgt aagggcgaaa agacagcatt
     6361 tggattttct atgtcgatga aacccttacc ataggatgca aggtcttgac cgatgagatt
     6421 tatttcaaat acattttttt caagcaagtc gataacttcg gcttttatat cttcgatact
     6481 gcgacttcga agatttcctc gaataagtgg aatcgcacaa aaagagcaac aattattgca
     6541 cccctcggta atttttacat aggctgaacc ggggaagtta aacaagtcat ttcgttttgt
     6601 agtagagacg ccttcttgtg gcggagtgga aaaacgaaac tcatcgtaac tgggcgcgtc
     6661 aaattcagga aagagttttt ttacgatttt ttttatttca cttaaatcgc cgttccccat
     6721 aaaaaaatca gcttcagaca tttcttcgct caattctttt gcatagcgtt cagagagaca
     6781 accggcaaga ataatttttt tatcgggatg tatttctcgt gcatgtaaaa gcgcttctat
     6841 cgattctttt tttgcgcttt ctataaaacc acaagagttt attataatga gggaagcctc
     6901 tgttgaattt tcacatcgaa gccagccatc ttcagataaa aaagctgtta acacttcacc
     6961 gtcaacttgg ttttttgcac aaccgtattg gtcaagaaaa aaaagttttt gcgtagcttt
     7021 aggcttagtc aactaaaagc accattagct taaaacgccc gtcgatatct ttaacccatt
     7081 taatatcttg gactaagact tgaccagggc gaccaacatc taggtcatga gtttttccgt
     7141 caccaattac ctgtatcttt agggcatttg catttgacat ccataagcga acaccatttt
     7201 gtgctgtaat tgtttgtaaa tctcccgcag aaaaataatc ttctattatg tctttattat
     7261 ctgcttgcga acggaagaga caagaaccac ggaatgtgat gttgacagta aatggatagg
     7321 cacgtgtacc atcaaaaata atcgactgtg agaggttttt atcaacctct gtgataagtg
     7381 gaatgccatc aagggaaagg ggttgttctc tcatttcagt ttcagataaa gcaaacatac
     7441 gaatttcagc accacgactt gcgtcgtttt ttgcaatgtc agaaacaaag atgacaatat
     7501 ctggaccttg aacagaatct atgtcaagtt taacctcttc acccaattgc acttgctgct
     7561 tgccgtttgg tgtaccaagc acaagttgct cctttgtttt ttcgacaagc aattcaactt
     7621 gcccagaagc gagcataacg gaaataatat cgttttcata gacgcgtttt tgaataggaa
     7681 cacttgttag tgtaaaaatc tgcacctcat ctgttttttc taagagctcc acacctgagt
     7741 cattattgat atgccgtatg aaaaaaaaga gaccgagacc gacaaggata gacaaagcta
     7801 taccaagtgg cagaacaatc ttccatggaa atgttttttc atcacgtaaa agcccaggtg
     7861 gaataggtgc ttcttgtatt ttttttgaac gatataattg aataagatac tttgcatcga
     7921 ggtttaaata ttctgcataa tttcgcaaaa acccaacaag atagggttca cctggaaaaa
     7981 catctgtttg ttccatttct aaagcacgaa tatattcgat ggatatactt gtatcatgtg
     8041 atactttttc taggtctatt tctcgttttt ctcgagctat ttttaaaatt tcaccaaaac
     8101 tttccatgat tagtactacc ctattctgta aaaagaaaat tattatatgt atttgctgtt
     8161 cccggagaat cgtaaataaa acgagtatca gggatttttt ggtttattgc ataattataa
     8221 aaagtaaaga caaagctttc atttgcaggt gtagttgcct ccacacgacg aatcagtttt
     8281 gtcgtaggat ttatcgataa cacaatggtt cgaaaacctt ctgaagcatt tcttcgtgaa
     8341 agcaagagtt tcatcaccat ctcacttgaa ccatttggat ttttttctaa atcatctaat
     8401 gtaaccgggg tatttttttc tgcataggat ataatataat atcgactcat aagattgaga
     8461 ccttgtgggg tagcaaggtt tgccccccct gctgcccctg aatccttgga aacagactga
     8521 gttaaaaccg cagaagagcc cggtagataa atcgtcaaaa gactaccgtt aaacaggatc
     8581 acttgtgtat cgggtcgaga aaaatcgata cgtaagagat tgggtttttt aaaagacacc
     8641 ttcgctgaca tattttgtga gccagcgcgg atttccaaat ctgcttcata atcggtgata
     8701 ttggcataat attcggagat actttgaaaa aagttttgag cggtttgtat ctcttgcgca
     8761 agcaaagaat tgagagaaaa acaagccata agaacaacac taatataaaa aagagtcttt
     8821 tttcgcatac gaattacatt ttccttatac tctataataa atcagaaaag tagactagtc
     8881 aaggtcttag aaatcgctct tatgcacttc agcgagataa ttaaataagc gaattgtttt
     8941 taagccatcg aaacgctcaa aaaaagcctt tcgtagattt tctttcgaag aaaaatgtgt
     9001 ttttacaatc ttctcccaat ctatccaaaa cctatcttcc gctaaaaaag catatacttg
     9061 tgaagagctg ttttttactt ttttttcaaa ttctaaaagc atttctatgc tcttttcctc
     9121 tctctcataa aaactcaaaa gcattgtatt aaaaagacta aaaaaatcct tagctgctat
     9181 atattgtttt ttttcaaagc cttttttttt ccctcccaga atctcttcaa gagctgtgcc
     9241 agtaccaaat aaaacacgtt ttgaaaggcg tgcggaggga tagacctttg aagcgagaaa
     9301 aagatagtct tctattctct tttttaagtt aagctttata agcgattgca aaaaataaaa
     9361 atcttccccg gcaatttttt tattcatacc accagaacgc acataggctt cgctcgtgca
     9421 tataatagtc ggtcccaagg caaccggata atacggactt tctatatcaa ataaaaaccg
     9481 actatgctca tgtaaatagg tttcgtaggc atccatagcc tttttatgtg ctttttctgc
     9541 tggctcttga tggctaaaac cacaaagcgc ccactcactt ttattcttgt atttttgata
     9601 aaacaacgca agcgtataca aatagttttc gcttacaaga caatcagcat cgagggaagc
     9661 caaaacttct gccccagatt taagcgccca gtcgagagct atttttcgag caagtcccac
     9721 cccatctttt tttttgaaat aggctccttc atgagaataa tcgagtatat gcaggttcat
     9781 aaaaggccca tcaaattctg ttttttgtaa acgccgcaat tcgtgtagca gttttgcgtt
     9841 gttttctttc acttcgagag catcgttttc tcttgcatta atacaacaaa caatactaaa
     9901 ggcttggtgt ttttctccca aatatgaaag agctttcatg gcagatagat gtaaggagcg
     9961 aagtgttcga aaaaaatgag gatattcata cgagacggga atacaaagag caataggatg
    10021 cattggtatt ttttcaccac agtttaaaag agaaagctca agtttttttt caagatattt
    10081 ttgcattgcc ttggacaaag gcttcttgga aaggattttt tttgaactca gattttcgag
    10141 atcatggctt tctaataatt cctctagatt tctaagctct ttttcttcca taattaaacg
    10201 agatatttct ctctatacaa gatatcaatt gcctttgcaa atcgcccttc gatcgaaacc
    10261 tcggtctcgc taccatcaca gggaatccag tgaatagcta ctccctttcg ttccattcca
    10321 cgaaaccatg tttcttgacg ttttgcaaac tgatgaattg cggtgttgag ttttgaaaaa
    10381 agcgcatcct ttgtaagccc tccgagaaga tagagcgaca agaatttata ttcgagtcca
    10441 agtctttgta agcgctcatg gctaatccca gagcttagaa gattttcaac ttcttcgatc
    10501 attccagatt ccaagcgttt tttaagacgc tctgtgatat ttttccgcaa aacttcacgt
    10561 ggaaaactgg ttccgagcac aagggctttg aactctgctt tactttcatc gctttgaggt
    10621 tttacatcac ccgcttttat cgattgtttt ttttcggcat caagtccctg ctctttataa
    10681 tagagctcta tttcaatagc gcgaagtaaa cgatgccgct cttccaaatc tgttttattg
    10741 tgtaagtcag gtttcatctc gcgtagaatt gcctcaagct catccattgt ttttccgttt
    10801 aagctttcac gaaggcttat atttgtcggc accttgataa ggctatagtc tcgaataata
    10861 gcatcgagat acatacctgt gccaccgaca atcaccggtt gaatagtttt ttttgcaagg
    10921 ccattaaaaa cagaaaaaaa atccttttga taatcgtaga cggagtattc gtccctgaga
    10981 tctgcaatat cgatgaggtg atagggcaca gttatttcat caaggtcttt tccggaacct
    11041 atatccaatc ccttgtaaac ttgccgtgaa tctgcggata taagctctgt agaaaaatat
    11101 tctgcaagac ggtttgcaag tggcgttttt ccaacagcag tcggaccgag aaggacaagg
    11161 gcattgtatt tttctgtatt gaaggaatat tttttctgca tctatgagag catatcttcc
    11221 atggtataga ctctgccctt ttggacggaa ccttttttta agccgtcaaa aagccaagag
    11281 gcagcatgaa cagcgccgag tgcaaagcct tctcgagagc gagcgcgatg aagtagctca
    11341 atactatctg cattggaatc aaaaaaaaca gtatgtgttc caggaacaga accacaacga
    11401 gtgcttgaaa catgtaattg atgggctttt ggtttttcat gaaatgcatc aaagtgcatt
    11461 tctgtttttc cactgtgatt ttctaacaca agttttgcaa gttctagggc tgttcccgaa
    11521 ggcgaatctg ctttttgatt atgatgtgct tcccaaagag ctacatcgta atcatcgtaa
    11581 gatgaaaaaa cctttgccgc ttcggcaacg accttgtaaa ataaattgac acctatggaa
    11641 aaatttgctg cgtaaaaaag cgaagaacta tgttttgcaa cgaggtcttg cacttcagaa
    11701 agatgagaaa gccagcctgt tgttcccacg acaagcggaa ttccaagcgg tataattgct
    11761 tttatatttt caaatgcggc atcgggatga gtaaagtcta taactacttc gctttttgtt
    11821 tcttgcaaag ccgctacaat atctgcagga gatgaaataa aatgacttgc atcctttgca
    11881 tatgaatcga tagttgctga aatttcaaaa ccttttttct tagcttctac ttctatcata
    11941 tgtcccattt ttccatagcc aagtaatgct atcttcatct tataccacct cttctaataa
    12001 ataagtttgt tatctataaa tgatttatgt aacacttgca caacttcgtt aacttcagtt
    12061 tcttcaacaa gcatactcac attcaccttc gatgcaccct ggcttatcat ttgcacattg
    12121 attgcattat cttgcagggc tttaaaacaa gcggacaaaa tactactcga acgttttaca
    12181 tcgcaaataa ccgttacaat tgctttcttt gttttgagtt ctacgtcagc gacattttgt
    12241 aagtccgcta taagcggttc caaatgttgc tttgtattga cggtaagtga aacagaaacc
    12301 tcgcttgttg caatcacatc gatgcttata tcccacttga gaaaattgtt aaaaatatgt
    12361 gcgagaaaac ctgaggcgcc gagcatacgc gttgaaacaa ggtcgattat cgtaacgcct
    12421 tttaccgcgg taatagcgcg cacaggggaa acttcaccct tgtgtttttc tacaatgata
    12481 gaaccagggg aagcaatatt ataagaattt tttactcgaa caggggtacc tgtttttcta
    12541 cacggttgca ttgaacgagg atgtaaaact tgagagccaa aataagcaag ctcagctgcc
    12601 tcttcgtagg ttacctcttc gatggggcgt gcatctttta caatacgtgg gtcagtcgtt
    12661 agaataccat caacatcctt ccaagtttgc acttcttcaa cattgagggc agctccaagc
    12721 atagtcgctg ttaaatccga accaccacga ccaagcgttg taatatatcc atttatatct
    12781 tttgcaataa atcccgttac aataggtatt tctttttgcc ttcctgcagt atagtcttca
    12841 aaagcctttg ggatagtagt ccacacagag tctttgagct ccgcactcat aaaatttgaa
    12901 tcggaaataa agcctgcatc ccaggcgtca taaaatcgag cagcacttcc ttttttgtct
    12961 aggtagagcg ccatcatacg tacagataaa cgctcgccaa atgaaacaag ataatctctc
    13021 gtgcgttttg taagctcttt tagcatagaa atacccgtca ggagagtttt cagctcttcc
    13081 aataacgctt ctatttctct cgcatcaatt gcaagttctt ttgctgtatc gatatgtagc
    13141 ttttcaacag aagaaatatc cacccttcct tctacagcaa ggtcggaggc ttcaagcaaa
    13201 aaatcagtcg tatctcccat ggcagagaga acaacgagag gctttttttc tttataggct
    13261 tctataatag aagcaacgtg ttgtattcgt tcagctgtgg caagagaaga gccaccaaat
    13321 ttcattacta tcataaaaaa atactatcat atttttattt tttagactat agttatgaaa
    13381 tgtttagaaa aggcaaccta ataatttaca aacaaaaacc cgcagtcatt caagcgatag
    13441 aagataaaat ctcaatttct tttgtatctg gttttaaggg gaaaaaagcc ctcatagaaa
    13501 atcaaaaggt acgcgacaag gatattcttt ttttacatcc cggtcctgct gaaaacctta
    13561 caagccttgt aacagaagat aatataaaag catcggatac atttacaaag gcaattatag
    13621 aaacatggga actcttagca gaagaagaac aagcatttcg atttgatgaa atagctgagc
    13681 tgagtatcga tacaaaggta ctagaaaaca tctttcccct atactttagc ctcatcgatt
    13741 ctgtctattt taaaacggag caaaaagaaa gccagctctt ttttattcct cgatcaaagg
    13801 atgaggtagc cgctctttta gaaaaagaaa atgaaaaaca aaacgaggct gctataaaag
    13861 atgcttttat aaaacgattg cgccaaaaaa aagcagactt agaaagcgat gcaaagtttt
    13921 tacaagaaat agaagccttt gcactcggca aaacggataa accaaaatat ctcaaagaag
    13981 cgcatgtaaa agaaacgatt gaagaagcgc acaaactcct tttagatgtc ggattttgga
    14041 caatgagtaa aaatccttat cctgcacggc atgggctttc gatgcattcg agtacgagtt
    14101 ttttagactc ccctccccat gaagacgacc gcataaaact cgagcacctc gcctatgcaa
    14161 tcgataatga aggtagcacc gaccctgacg atgcaatagc ctatgatggc gagcacttat
    14221 ggattcacat agcagaccca gcgtcaatag tgctacctga ttcagctata gataaggctg
    14281 cacgaaatcg cggagcgacg ctctatattc cagaaggagc aagtcgcatg ctctctgaaa
    14341 aaagtctcaa ggactatgcc ctcggtttag aaggagaaaa ctcctattct cgagcgctct
    14401 catttaaaat acgcataaca gaagatgctg ttgtcgaaga tgtaagtatc cttaaaagct
    14461 ttgtaaaagt aaaacgcctt tcatataaag aagctgatga gcaaaaaaac agctcccaac
    14521 tcgccccact ctttcgcctt gcagataaaa acttcaccaa acgaaaaaat gccggtgcaa
    14581 tacagatcca gtttccagaa gcctcagtta ttcttacaaa aaatgaagga gctgatccaa
    14641 gtgtcgaaat tgcaccactt gtagaatacg aatcctttgc ccttgtccgc gaaatgatgc
    14701 ttttggcagg agaggctgca gctcgttttg cttttaaaaa caatattccc tttccctatg
    14761 tctctcaaga agaacccgag attcccaaaa aattgcccga gggtcttgcc gggcaatatc
    14821 gcctcagaaa aagcatgaaa gctcgctctc ttggtgtaac accttcgcaa cacgcaagct
    14881 taggtctctc tttttatagc caagtttcaa gcccacttag acgctactcc gacttagtcg
    14941 ctcatcaaca aatccgtctc tttttagata acaaaacagt attgacggga gatgcggttt
    15001 tggaaagaat atctcaaggt gatgctgcaa gccgcgctat aatgagggcc tctcgggaaa
    15061 gtgagcttca ctggaaactc gtctaccttt tacaaaactc tgaatgggaa ggaaggggta
    15121 ttattctcga acaaaaagga aaaacagcca ttgtttttat agaaagcctt gcaatggaag
    15181 cgagcgtaca tgctccgaaa gcagagctca acgacaaaat ttttcttcgt gttggaaaaa
    15241 ttcagctgac aaaactagaa gtacaattta tagaagctta aaaaactttc ctttaacaaa
    15301 aaagctgcca acacaaaaat gatggcagct tttcaatttc caaatactat ttattcggat
    15361 tttacaactt cacagatgtt tagttctgta tctttatcaa agaactttcc tttttcgaga
    15421 agtggtgtaa agtttgctgt gtccccgatt tgatatttgt tatcaggttc tacaacagcg
    15481 ataacctgtt gtgtcttcgt ttgaaggtga agatgtgttt ctgcaccaag cggttcaata
    15541 acagaaattt tcatttgcat attatttact ggtgctggtg tttttacata ggttaaatcc
    15601 tcaggtcgga atccaaaaaa tacttctttt ccaatgtagt ttttaagcgt ttttgcttgc
    15661 tcatcggttg gaattatttc aaaagagcct tcgtcagcaa tcaattttcc gcccttttca
    15721 ataattttta cagtaaggaa gttcataggt ggagaaccga taaaacctgc aacaaattta
    15781 ttgattggat ggttatatag atacaaagga gatccaattt gttgaatctt tccgtctttc
    15841 ataacaacaa ttttatctga catagtcata gcttcaatct gatcgtgtgt aacgtagatc
    15901 attgtagcct taagtctatg gtgaagatca gaaatttcag cacgcatttg tacacgaagt
    15961 ttagcatcaa ggtttgaaag cggttcatca aacaagaaaa cctttgggtt acgaacaata
    16021 gcacgtccaa cagcaacacg ttgtctttgt ccaccagaaa gagcctttgg ctttctatca
    16081 agtaattttt caatgtcgag aattcgagct gcttcattta ctcgtctttc aatttcagcc
    16141 ttatctgttt ttcgaatttt aagaccaaaa gccatattgt catatactgt catatgtgga
    16201 tacagtgcat agttttggaa aaccatagca atgtttctgt cttttggtgg aacatcgttc
    16261 attaactcac cgtcaatgta caattcacct tcagtgatat cttcgagacc agctaccatt
    16321 cggagcgtag tagatttacc acagccggat ggtccaacaa agacgacaaa ttcttggtcg
    16381 tctacaacaa tgtttgcatt gtcaactgca cggaaaacgt tatcgtatac ttttccaatg
    16441 cccttcagtt ctactttagc cataaagcaa aacctcctat cttatattat acatttaatt
    16501 ataaacctaa aaaacaaatc tgtcaaacta aaccagagat ttatagataa aaaagttaaa
    16561 caaaagtgat attgtcctaa ggaaacgcaa aaaccaaaaa aagctatctt tatcgatatt
    16621 ccttcataaa aaatataaaa tatataataa ctgctaagag tggcaccaaa gaaaaagaag
    16681 taaaaagaac agggtatccg aaatattcta taacaagacc gccgatgagg ttgccgatta
    16741 tgctacccgc acctattgca acgatagaat ataaggtcat tccaacaaca agatttttcc
    16801 tcggcgtgtg ttctgcgaca aaaaaaattg cggctggatg atacaagcca taggtaaagg
    16861 cgttgaaaag ctgggcaata agggcaccgg taaagtttgg tatgagaata taggtaataa
    16921 tccgcacaaa gacaaaaaaa gtgccaaaaa caaggagtct catactaccg tagcgacgta
    16981 aaaatcgatt tgcaaaaaac ataaaaggta tttcggcaat agctgcaaga gcccataaga
    17041 aacctgaagc ttgtaagcca agatattcat agatatacat ggaaaaaaaa cgttgagatg
    17101 gggtcattcc taaatagcca caaaaaataa gaaaaagacc tacccaaaaa actttgggga
    17161 agtttttaaa tgcgttaaag aaggattttt tttcctcgaa ttctgtttct acatgggctt
    17221 ctgcatcagg aggctgtctt tttattaagc ctggaataaa aagaattgaa agagcaaaaa
    17281 gaagggaggg tatacacatc cagaaaataa tagtgttttg tgatgcctgt tctggcttta
    17341 caataaattg caaaaacaag ctcataagca caaagcctat ggaacccata acgcgcactc
    17401 tgccatattt tgtatgctca tcaccgagaa ggaggtttgt catagaatct gaaaccggta
    17461 caataccttt atagcctgtt gcatacatag caagcagtat agcagtcatt gtaaaaccgc
    17521 ctattttaac aagaggaaaa ggcaaaaggg ataaaataat gcctgaaata aataaaaatg
    17581 gaccgtgcaa gccttttttc gatataaagg ggctcagtaa aaaaggaaaa acaagacctg
    17641 caacttcaaa aagggcaagc aagtaaccga catggcttgg ggtaaaaccc atggaccgta
    17701 aaacaagggg taaatagaca ttgattacac cataaattgc aaaaagtaaa aatatggggg
    17761 cagacatttt cataggcatt tccttaattc actagagtat aaatatttat attttacttt
    17821 gtaatttttt atggaacaaa gaaaacaaga gctgataaag gcgcaatatc gattgttttt
    17881 cctccgtatt ctgagctatc gaaagaaaag ccatctacac ttactctctc cccatgaaca
    17941 taaacctttg cctgagaaaa atcaatattc aattcaacac tagaaatagt ttttcgtgca
    18001 ttaaaaataa ggtagcaaac ctcatcaaaa ccttggtgtt taagatttat agtatacacc
    18061 aaaagaaggt cggagctttg tgtttgtaaa aaatgaactg aagactcaac gagttcttta
    18121 gtatgcaggc gaaaatgcgg catttttttt ctcaaagcaa taagtgagcg aacaaaggca
    18181 tttagttttt ccctttgtgc atctaaggtc cacgaaaaag agtttatagt atcgcttgct
    18241 ttatatgaat tccgaacaaa attgccatgt atatcggaac tatcaaaatc tctaactgaa
    18301 gcttttggct ttgtgcgtgc attttcctgc cctgcttgta taaatggtat tccctgactc
    18361 gtaaacaata aaacatgagc aaggcgaagc cgcgcgtaaa gctcatcaag atgcatacta
    18421 ttcgaaagat caaagttcat attgtgtgct ataacgtcat gtaaagttaa accgtcatgg
    18481 cattcaaaat attgtacaga acgtgctgga ctattcactt taaaattaac ttgcggaaga
    18541 gcacagacat tgtaaaaaag gtcctttgta aaaatggagc cgccggttaa aaaacctttg
    18601 agatcttcat tcgtccctcc gcctttggca aggtcgcgaa actcatcgtt gaaaacagag
    18661 actttttgtg tttttgtcat aaagttttga tccatgccct gcgtaccctt tggaccatta
    18721 tacattttcc agccttcccc aataaacaag atatctttat tgtataaaac acaggcgtca
    18781 taagcatcta aaactgtttg actgtccatt agtcccatga ggtcaaaacg aaatccatct
    18841 acgtggtatt ccttaacaag atatactaag gagtcgtgaa tgagttttcg agccattatc
    18901 cgttcacttg ctatatcatt tccgcaggca gaattattta aaaaactacc atcttgtttt
    18961 gataatcgaa aataataatg cggaacgatg tcatccaaaa aatctatttt tgccatatgg
    19021 ttgtacacaa catcgaggat aacccgaagt ccttgtaagt gcgcttcctt tatgagcgtt
    19081 tttaactcgg atatacgaca atagggatca tgaggatttg aagcatacca accctctggt
    19141 gtaaaataat tgtgtggatc atagccccag ttataattat tattgtacaa agctgttgat
    19201 ttttcaaaat cgagctttgt ctcatcgttg ttataaaaat ttaatacagg caataactgg
    19261 atatgcgtta tgcccatttt ttttaggtag ggcagttttt gaacaaaggc attgtaagaa
    19321 cctggatgtc caagtaggtt cgccttatct aagcgagcgt caggagatat ggtaaagtca
    19381 cgaacagata cctcatagat aattgcatcg gtgtagtgat taaaagcagc taaggcggaa
    19441 agattttctt cgtaagaatt ttccttgctc cacccaccct ttggaagata ggcaggagaa
    19501 tttggattga taatagcagc tctcccgctt gtattgtcat ttgtaaaggc tgccattgat
    19561 agagcatagg ggtcgaggca agagaggctt cccttttctg tagtaatttc atactcataa
    19621 aacatagtgt cgaggggaac atccactgag caacgcagtg tccaaactcc tgtttttcta
    19681 tcgattataa gagggaaatt ataagagaag tgctcggttt ttccgtcatc aaaaagaagt
    19741 acgcgcacct tgctcgccgt cggtgcccaa atagaaaaat gtgcggtatg cgtttcttcg
    19801 tcataaaagc aacctaaggg agttgaaaag gaaggtatcg ttttttcgca aagcttccaa
    19861 agtctctgtt ttttcatagc ttattctaag actaaaacaa aaaaaagaca atggaccata
    19921 gcattgtcct ttttcttttc ttcttttata ctgagctaat gaaaaaaaat ctctattttg
    19981 attgtgcagc gactgcctta tatgatgaag ctatcataaa aaacgctttg gaaaaaagct
    20041 tgtgtttttt tgcaaatccc tcaagtcttc acaaaaaagg acttgaggca aagcaatgct
    20101 tagaagcatg tagagaaaaa gcggcaaaat ccttggaaat acctgcagaa cagctttttt
    20161 ttacatcagg cggaacggag gcaaatcaga ttgcgattct ctcactttta actcggccaa
    20221 gcaagggttc tatacttata tcggcgatcg aacatccagc ggtacgcgaa caggtaaaaa
    20281 tgcttgaaag ctctggctgg tctgtacaga ctatccccgt caatactgag ggtagtatca
    20341 atctcaggag tttgagagaa aaactctcgg cagatacagc ccttgtttct gttatggcgg
    20401 taaataatga aacgggtgca atacaaccga ttgaaaaaat aggagctctt ttacaggagc
    20461 tctatgaggg gaagcgaaaa cctcgctttc acgttgatgc ggtgcaagct ataggtaaaa
    20521 ttcctttttc tttaaaacag acagctatcg attctgcggc attatctgcg cataaaatag
    20581 gtgggccaaa aggcgtcggc gccttgtata caaaacacca aatggaaagt tttctaaaag
    20641 gcggaaatca agaaagaggt ttacgaagcg gaactgaaaa tctttttggt atctatgcct
    20701 ttgccgattg cttggaaaaa tatacgatag gggaaaaaac gcaggctttt gagtgctatg
    20761 aaaaacaaaa aacactcact gcttttttta tagaagaagt aaaaaatatt aaagctgcta
    20821 caattatacc aaaaacgcgg acaggaaaag aaggtgagtc attttctccg tggattgtcc
    20881 aagccgcatt tcaaaatatt ccagcacaag tgcttattcg cgcactcagc gagcatggcg
    20941 tttgtatttc cagcggttca gcctgctcct cgaaaaaact ttctcgacca gttttagaag
    21001 caatgaatct tccaaaagag cagatacaaa atgcggttcg cttttccttt tgcccaagcc
    21061 atacgaggga agatattgaa agcctgctag cattgctcaa gaacaccgtt aatcaatatg
    21121 gctaaacact ctgagtatat cattttctcg ctcttgtaaa tcttgaggga aattgacata
    21181 ggttttagaa gcgctttgtt tttgagattt ttcttgcgtc atgctatgaa gtaattcttt
    21241 ttttataggg ctacctttta aaaccataat agtatctgca agacttaaag cttctcgaat
    21301 atcatgactg acaaggataa tcgttcgcct atcttcttcg tgtaagctct gtagtagatc
    21361 catgagttgc aattttagca caatatcttg agactggaag gcttcgtcca taagcaagag
    21421 tggtgatgga aaaagaaagg cacgagcgag ggcaagtcgc tgtcgctctc ctccagatat
    21481 ttctgagggg aaacgttttg cgtagtcgat gagatgcact ttttcaagca tggaagctac
    21541 tttttctttt gcaagatttt tatcggtgta tagtttttca ataacaaggg ataagttttt
    21601 ttctaagctc agccaaggaa gcaagcgcgg ttcttgaaat aaataactaa gcgcggtaat
    21661 tttagatatt tttccatcat cgatcggaag caatccagca atagcgtgaa gaaaacttgt
    21721 ttttccgcaa ccagagggtc cgagaatggc atgaatcaaa gaggcgtcaa aaacatgtga
    21781 aaaatctcta aacagcagct tatcgtttcg agtgagagag actttctcta ttttgaggct
    21841 aagtgccttc tcttgcgtgt tatttgaact aggcatcttt ttctctccac ttcatacagt
    21901 ttttgaaaag ggcagataac aacaactcta aaacaaaact caaagcgata attaaaaggc
    21961 taatagcaaa gacgtcagct gtttctatat gcacctttgc agtttgcaaa cttgtaccta
    22021 tagcacgctt tggcaagctt atcacttctg ctgcaacaac aaccttccag cttaaaccga
    22081 aggctgatag ggcgccggaa gcaaaggaag gaaagacaga aggcagatag atatgtagga
    22141 ttttttgctt ttttgtaagg ccataaactc gggccatttg ccttagtttt atatcaatat
    22201 ttgcaatgcc gagagcaacg gaagatgtaa ttatgggcag ggtcataagc acagaaacaa
    22261 agatgggcac ttgagacgtt gtaaaccaaa acagcgcgag taaaataaag gagacgacag
    22321 gagttgcctt gataactgaa agaggcattt taagtaggag agaaaaactt ttactaaaac
    22381 cgattgcgat accaagaata attgaaatca caagcgaata tagaaaagaa aggattgttc
    22441 gcaggatagt ataaaacaca tgttcccaaa acaagttttc ttttataagt aaaagaaatc
    22501 gcgtaaaaac gaaaagaggt gatggtaaaa taagactcga agcaatatga attgaagcga
    22561 gataccaaac gcataagagt atcgccgttg gtataaaaaa gacaaaaagt atttttttca
    22621 ttcgcttcaa ctcatatttt tatttactta attaaaataa aaactgtcgt caggtagttt
    22681 cccaccaatt gcttcgggcg caaagtctaa aaaaacagag agtaagtctt ctatgtcttt
    22741 ttttgcatca actgcactgc tatatgcaaa agcagaagtg ggaatagact tagttacaat
    22801 tggcgctttt aagcccaagg aatatttttc aacaagctgt cctgctttct cgggattttt
    22861 attggtatag tctattgcag ccgcaacttc ttttaaaacc tttttcacca agtctttatt
    22921 ttcttctaca aaagatttac gaccaaccaa aaccgtcata
//
LOCUS       DAOSSW010000020        22744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_2448_length_22744_cov_7.490149, whole genome shotgun sequence.
ACCESSION   DAOSSW010000020 DAOSSW010000000
VERSION     DAOSSW010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 22744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22744
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2448_length_22744_cov_7.490149"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2278
                     /locus_tag="PKW26_03110"
     CDS             <1..2278
                     /locus_tag="PKW26_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ92590.1"
                     /translation="KAKPAQTGSNATNPLVLSVSTLDGKFSPFFYTSAYDNEVQEKTQ
                     LGLLYYNKEGAPEAGMAVPCLAWDYSQDVNADQSLTTYTFILKDKIVFSDGKPVTAKD
                     VLFSIYTLADPFYDGSSTFYTMKIQGINEYRLQTSAEVLKNVESILAAGVGVDGSGKL
                     LPNPVSGISADQVERFWSYVDEGGVKFAQEIVDYVLNNYLSYVGSDYFPGFTADQVKA
                     SASLQTAFGMAMWGFGEVVDGSIFEDANGVEYNLDTDTLDASIYWDNIVGAYGYDLSD
                     GGINAEKAGDLTVQDYTKDAYISAEGQIEGGVKSITGITSGKMKCDDGVTREYIQVVI
                     DGVDPKAIFNLGILVAPMHYYTAGFKGKLNEFGVATANREFIEVLKSKNSNPVGAGPY
                     IFKEYKDSVVTYTANDKFLLGAPKIKTLRLKEIALGSELDSVLTGEVHFTDPSAQNSI
                     INNISTGDGNYANLAYTLVDNDGYGYIGINAQAVPEFEIRRALAHAMNVQLAVDNYYQ
                     ELASVNYRTMTKILWAYPDNPTNLFPYDGTGETSKALFLEAGYTYDAAKNIMSYPAGH
                     EKAGQQVTYKFTLPSNASDHPAGSIYIDTQDVLSKIGVKVNIDVDQNLLGKLDTAYES
                     GVVVWAAAWGSGGVDPDMFQIWHSDPKQNQSTSPVAKGLYWMFQNGSDEEKAMLNELN
                     DLIIAGRSTLDVEERKAIYGRALDLSTGLAVEIPTYQRKNLYVYNKKIVKASSLFSGN
                     DVTPFQSPISFIWNVELN"
     gene            2344..3342
                     /locus_tag="PKW26_03115"
     CDS             2344..3342
                     /locus_tag="PKW26_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ92591.1"
                     /translation="MWKYLLKRISLALLILVGVSALIYLLVMLMPADYIDNQTSAALQ
                     SGAMTKDDVLRLKELYGLADKSFFGITKSYGKWLGSSLSGDFGFSFVYGKPVTQVLTS
                     YMWISFLIALIAFVLQMIIGIPMGIKAAVNQYGAYDYTVSIFAMIGTALPSFFLAALL
                     MNVFAIQLNILPLQGLTTATKIFAETAHLQIFFDKLLHLILPITVLTLLGVGGLMKLT
                     RTNMLEVLNSDYIRTARAKGLSEHVVTYKHAFRNTMIPLVTTLSGMLPSLFGGAMITE
                     TVFAIPGIGYMALRATQQGDIPFIMGYNMFLAILTVLGTLISDFMYMVVDPRVKLS"
     gene            3354..4397
                     /locus_tag="PKW26_03120"
     CDS             3354..4397
                     /locus_tag="PKW26_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ92592.1"
                     /translation="MAEDTKVTKKQESAEEMHFQVISPGRMVAKRFFKSYLSIIGLAM
                     IAFVFIFSFLGPVIVDMTWKYKEIQVFKIDRPNDLILTADFIGADGKTYTYYDRSQTI
                     VQFKAPLSKQHWLGTDTSGFDIFVRLMYGGRISLTISFIVIFLETFIGVILGGIAGFF
                     GKWADMIIMRIVDIFSCLPALPILLVLSATISSIESIPTEHRIYYLMAFLTLMGWTGV
                     ARIVRGQILMLREQEYMMAAETTGIKTFNKIFKHLVPNTIPQLVVSATLGLGGIILYE
                     STLSYLGLGVPFPRAAWGSMIALADPSKGQEILAMYPNMWVPAGILIVVTVLGFSFIG
                     DGLRDAFDPRMKR"
     gene            4423..5643
                     /locus_tag="PKW26_03125"
     CDS             4423..5643
                     /locus_tag="PKW26_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ92593.1"
                     /translation="MGFFNFKNKKIQLEDDIDETMYLSVKEERRIAKENKKVFRDYTK
                     LKRRKNVSEEEYLTQMRDSANVVEFDDLHTYFYTDVGTVKSVNGVSFDVPQGKIVGVV
                     GESGCGKSVTSLSLMQLVQAPTGQIVSGSIRYQDATGKCYEITDMPTDAMLHIRGKDI
                     AMIFQEPMTSLNPVFKIGNQMDEVVLLHVEDATSESAKARSIEMLNLVGIADVEKVYD
                     SYPHELSGGMRQRVMIAMALSCDPRLIIADEPTTALDVTIQAQILELLREVKDRIHGS
                     IMLITHDLGVIAEMADLVVVMYAGRIVEKGTVQEIFKNPKHPYTIGLMKSRPGSQNTG
                     DRLYSIKGQVPNPINMPPYCYFYNRCDKAFAPCSQSYPELMQFSKTHFANCHLYDPKY
                     ASGLKDEKREKQNG"
     gene            5636..6640
                     /locus_tag="PKW26_03130"
     CDS             5636..6640
                     /locus_tag="PKW26_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ92594.1"
                     /translation="MDKNNTAKNQDANTILTVKNLKKYYPLSNIEFGRQKKFVKAVDD
                     VSFAIQKGTTMGLVGESGCGKTTVGRTILRLHEVTSGQVFFDGKDLAKISSRQLRLLR
                     PQIQMIFQDPYSSLSPRLPVGEIIGEAVKEHKIVPKSEYRSYIHKVMSDCGLQSHHYY
                     RYPHEFSGGQRQRISIARAVALQPKFIICDEPLSALDVSIQAQIINLMKDLQDQYGYT
                     YLFISHDLSVVEYISDTIGVMYLGKLVEVASKKQLFENPMHPYSEALLSAVPVANPDI
                     KMNRIILKGDIPSPVNPPSGCSFRTRCPKVMPVCAEKEPLLKDYGEGHCLACHLYAQD
                     "
     gene            6652..6981
                     /locus_tag="PKW26_03135"
     CDS             6652..6981
                     /locus_tag="PKW26_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92595.1"
                     /translation="MKESRKETIDKKANSLNDDGRVFADMSIDGMPPSLFGGIKKAFG
                     VCGGKRCKEQKKTNSEKSFFKLDREEKKQRLAISLGVFSSYLVFTLVFFGIIALFFLF
                     SQFIWFK"
     gene            7023..8384
                     /locus_tag="PKW26_03140"
     CDS             7023..8384
                     /locus_tag="PKW26_03140"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOQ92596.1"
                     /translation="MKGNSFYKNRFVYISLVLLLIFALSSCGKKIRIVDPVELGFSCT
                     VYYNALGGTVNKREIRETYYEPGSFLFMPSGTSNMLIEPIREGYILAGWYKAKTDILD
                     ENGKVIAYDFKAEDRWDFAEDRVYDDMSLYARWIPQGKAEYIDAETGLVMFSKNISDK
                     SPVLALTRAAENLIKKKGYTFAGYFTNPDFTKPFDFSARQINALKPNEKDFEKEVTSL
                     YPQINLEKLSESEKILVRTVKNNLYEAYIQEYIENTKSQNIFLKYEKGLVIHVASLED
                     LRYKGQLSFSGLTVDGEPVDRYSIEKDIDFKGVSLVMGESFSGKISGNGHSLKNISLV
                     LNSKPIDKDKEKKLSLFENLEDAIIEDLHIENFVIKINANAGVKVMAAPLAINGKNLS
                     LKNVSLQNIQIDTGRSDDGSAEYLLGDLFVNSENISLANTKGSQFVFSHSSFAKVHRL
                     LTR"
     assembly_gap    8583..8682
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8683..9197)
                     /locus_tag="PKW26_03145"
     CDS             complement(<8683..9197)
                     /locus_tag="PKW26_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOQ92597.1"
                     /translation="MNQSKDEQLLKDLEILAKEKGLDIRDELQEITKKIQSSKDIVSK
                     VWKNVELARHSDRPRTLDFIEMIVDDFVELHGDRYFGDDLAMVGGIGFIDGIPVTVIG
                     NQKGRNLKETIDRNGGMANPEGYRKALRLAKQAEKFNRPIITFIDTQGAYPGLGAEER
                     GIGEAIALNLR"
     gene            complement(9194..9964)
                     /locus_tag="PKW26_03150"
     CDS             complement(9194..9964)
                     /locus_tag="PKW26_03150"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HOQ92598.1"
                     /translation="MECPHCRISHDSDLFEQHLMVCPECSRHLRMTVWQRIEYLTDKD
                     SFKELYKTLKSKNPIQMEGYEEKLQAAEAKSALKEAVLTGSCTIEGEKALLALMSFDF
                     MGGSMGSVVGEKISRLLLKGASERTPVIIYATSGGARMQEGLLSLMQMAKTSSAAAEM
                     DKAGVPLFIMLCDPTTGGVTASFAMLGDIICAEPGALIGFAGPRVIEGTIRQKLPEGF
                     QTAEFQLQKGFVDCIVPRSEQKAFFAKMIKAHSQGGEK"
     gene            complement(10031..11356)
                     /locus_tag="PKW26_03155"
     CDS             complement(10031..11356)
                     /locus_tag="PKW26_03155"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOQ92599.1"
                     /translation="MIKKILIANRGEIAVRVIRTCKEMGIETVAVYSSADKENLHVKL
                     ADKAYCIGPAPSSKSYLNAEALITIALQTGCQAIHPGVGFLSENPRFVRDVEKAGLIW
                     IGPKSDVIEMLGDKVQARATAIKAGLPITPGSDGAIKTEEEAIAISEKCGFPVIIKAA
                     SGGGGKGMRIVYKKEDLKENLTIASREAEANFADGTVYIEKYLVNPRHVELQVVADGK
                     GNVAVLGERDCSVQKNHQKLIEESPSPSVSPKMRQEMTEGAKNLFANLKYRGAGTIEF
                     LVSGDAFFFMEVNARIQVEHPVTEFVTDSDIVREQILVCSGLPMEVSSEKNKLKGWSI
                     ETRINARAPGKVQRLEVPGGIGVRFDSFLYNGYTVLPHYDSMIAKLIVHDTNRERALK
                     KMLRALDELVIDGVPTNIEEQKSILTSKKFMSGQFGTSLYTELFPDKTV"
     gene            complement(11366..11851)
                     /gene="accB"
                     /locus_tag="PKW26_03160"
     CDS             complement(11366..11851)
                     /gene="accB"
                     /locus_tag="PKW26_03160"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOQ92600.1"
                     /translation="MNEAFILSLLEKFDTSSVKELEIQKDGTKISLSKNDRAIQSQAP
                     VQFAPTATSQAAASPVATKNTAEAASEKKAAASSKLSDALTIKSPIVGTFYRSPSPDS
                     PAYVEKGKKVKKGDVICILEAMKMMNSLEAEFDCIIEDILVTNGELVEFDQDLFTVKK
                     I"
     gene            complement(11911..13143)
                     /locus_tag="PKW26_03165"
     CDS             complement(11911..13143)
                     /locus_tag="PKW26_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HOQ92601.1"
                     /translation="MIIKPMIRSNICLNAHPAGCKQSVLDQIEITKKVRAEYDKKNAS
                     KGKNLRPIKNVLVLGCSNGYGLASRITAAFGYDAATIGVSYEKAGTETKGGTPGWYNN
                     LAFDAEAKKAGLVSVTIDGDAFSDPIKQEVIQAAKEKNMVFDLVIYSLASPVRTDPET
                     GVMYKSVLKPFGTKYTGTTIDAMTGEMKEISAEPANEDEAMQTVKVMGGEDWERWIDQ
                     LSDAELLAENCLTLAYSYVGPEVSQAIYRRGTIGKAKEHLEKTAKVLTEKMANIKGEA
                     YVSVNKGLVTRASAVIPIIPLYLSVLFKVMKEQGSHEGCIEQINRLFWERLYLATDGS
                     EFAKIPVDEENRIRIDDWEMDPQVQAEVDRIMPLVTQENVGELADLEGYRHDFLATSG
                     FDIAGVDYEADTERFDRI"
     gene            complement(13162..13593)
                     /gene="fabZ"
                     /locus_tag="PKW26_03170"
     CDS             complement(13162..13593)
                     /gene="fabZ"
                     /locus_tag="PKW26_03170"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOQ92602.1"
                     /translation="MSVTKDIKSLIPHRKPFLFVDEILSCDAEGSVSEHIFTEDEFFF
                     EGHFPEYPVVPGVILVETMAQAGGAALSYQKTFAEGSLFFLATVDKVKFRRQVRPGDK
                     VRMEVKNLRVSPKMIKQSGKAYVGDEVAAEAEWMCLVGSAN"
     gene            complement(13594..14850)
                     /gene="fabF"
                     /locus_tag="PKW26_03175"
     CDS             complement(13594..14850)
                     /gene="fabF"
                     /locus_tag="PKW26_03175"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOQ92603.1"
                     /translation="MRRVVVTGLGAVTPLGNTLAETWSGVKEGKSGITKITQFDASDF
                     SCKIAGEVKDFNPSDWMDRRDARKMARFTQLAVAATAQALEDAGLTKDTIDKEKTGCI
                     LGNGIGGFEITEHSMKKYFDAGNARIPPLTIPLLIPNEAPGNISMIYGLHGMGFTIAT
                     ACASGTDALGLALDQIRSGRMDMCISGGTEATVTGFGIGGFSVLQTLPTSFNDNPTKA
                     SRPFDKRREGFVMGEGAGILILEEYESALKRGAKIYCELAGYGASSDAYHITSPDPSG
                     IGGALALKTALKDAGVKPEDVDYYNAHGTSTPINDPSETQMIKMAFGEHAYKMKVSST
                     KSMTGHCLGAAGGIEAILSVKAIEEGFYPPTINLDDPAIDEGCDLDYVPNKGIDASAT
                     GGISCAASGSLGFGGHNGVLIFKKVK"
     gene            complement(14852..15595)
                     /gene="fabG"
                     /locus_tag="PKW26_03180"
     CDS             complement(14852..15595)
                     /gene="fabG"
                     /locus_tag="PKW26_03180"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOQ92604.1"
                     /translation="MLLKGKTALVTGTYQGIGVEIVKRYLEEGASVWGVSIKPSPVKE
                     ELEAYAKSFGGSFTEIYADVSNKEEVVPVLEKALKDSGGFDILVNNAGITRDGLSFRM
                     SMKDWDDVIRVNLTSVFIISQVISSDMIKKRKGSIINMSSIVGLHGQGGQVNYSASKA
                     GLIGYSKSLAKEVAGRGVRVNLIAPGFIDTDMTQAIPEEAREKWLETIPMKKGGTMKN
                     VADSCVFLASDLSSYITAQIIGVDGGMGA"
     gene            complement(15608..16585)
                     /locus_tag="PKW26_03185"
     CDS             complement(15608..16585)
                     /locus_tag="PKW26_03185"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOQ92605.1"
                     /translation="MSTVFLFPGQGAQFPGMVKDICDAHPEAMRLVKEAEEISGEALS
                     EWLWNSDASLLSRSDRSQLAITVASLAIAKVLEIKGIKADAVAGFSLGEYPALYTSGV
                     LSFADTIRVVKERGRIMQEACEAIQNESSSSELDNASPGMAAVIGLETDEIIAILKEY
                     SGEKGIVFPVNMNSPKQTVVAGTAEGLEVCERLCKEKGARRFIRLQVAGPFHSPLMHK
                     AAKDFGNFLETISFQNPKTLFFSNVTGDIIKSGEEAKRRAVEHITYPVLWTSVEKKIH
                     AFFEESKSKAVLLEVGPGKVLSGLWRDSGYAEAITSIPCGTLEQIQSII"
     gene            complement(16694..17641)
                     /locus_tag="PKW26_03190"
     CDS             complement(16694..17641)
                     /locus_tag="PKW26_03190"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOQ92606.1"
                     /translation="MSVIIRAAAKALPKNHVINDDLAKKIDTSDEWIRSHTGIGARYI
                     AEEGETSASLGARSSEEALKKAGLEANQVDLIVCATATPDYHGFPANACLIQESLAAE
                     NAACFDLSAACSGFLYALDTAEALMAKHNWKYALIIGTEVLSSIVDWEDRATCVLFGD
                     GAGTILLENNAEIDKAERGLSSFILGAQGSGSQYLYNKFGEALQMDGRAVYNFAVGIM
                     TQLIKDLMEKENLTEDMVDYFVCHQANERILSAAAKRLGFKMDKFIISLDEYGNTSSA
                     SIPLTLVDMDEKGMLKEGMTIVSAAFGAGLTYAGAVFRW"
     gene            complement(17792..18553)
                     /gene="aat"
                     /locus_tag="PKW26_03195"
     CDS             complement(17792..18553)
                     /gene="aat"
                     /locus_tag="PKW26_03195"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOQ92607.1"
                     /translation="MRQKQKNENSNNFPYLPIDFFFPFPDPNETQDGVVCMDGNLSPG
                     MLLSAYLQGIFPWYNADEPILWWSPDPRFTILKKDFHIPKRLKRFIKQNKEKQSQKDP
                     TAFYYTVNTDFEAVITNCAHVKRVDQKHTWIIDEVIQAYTEFHRLGYAHSFETWQAGK
                     LVGGFYGVLIGKIFFGESMFTLKPEASKAIFAEFAQIFFDLGGEVIDSQIYTDHIARF
                     GGKNISRSAFLRLEKDLLFTKPKSSVIEHFQNHFS"
     gene            complement(18525..20861)
                     /gene="clpA"
                     /locus_tag="PKW26_03200"
     CDS             complement(18525..20861)
                     /gene="clpA"
                     /locus_tag="PKW26_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HOQ92608.1"
                     /translation="MKISQLVQNIIKNAFNEAKKQQHEFVTPEHLLKVFLDNKQILEF
                     LDWCGADLPYLTRSVDEYLKTRVPVAENKGQADPVQTIGFSSIIDRTLLHCASAEKKS
                     IEYTDVLVSLYDEKNNHCSYFLKNSGVSRLNLLENITYLDMLENSGVDVNENITDIEN
                     GVESALIADLEKLDELNKQNKKQKAKQNEKVKKTFLERFTTNLNEEAKEGKLDTLIGR
                     DEEIERTIQILCRRTKNNPVHVGDAGTGKTAITEGLAQRIVSGEVPDFLKKFKILSLD
                     MTALIAGTKFRGDFEDRIKKLGEELLKEQSTILFIDEVHGIFGAGSSASSGLDASNLL
                     KPLLAKNTFRCIGSTTHEEYAKTFEKDRALARRFQKIDIIEPTEAETIEILKGLQERY
                     ETHHQVQYTDEALEAAVVLSKLYITERHLPDKAIDLIDEAGAYLRLHPTKDQKVTKQL
                     IEKTLSKITRIPEKTVSSDEKTSLKSLDSDLKKEIFGQNEAIEAITKSVKRSRAGFRA
                     PEKPIANFLFAGPTGVGKTELAKKLAELLNISFLRFDMSEYQEKHTVSRLIGSPPGYV
                     GSEDGGLLSDAVRKNPHALILFDEIEKAHPDIYNLLLQIMDYASLTDNHGKKADFRSS
                     IIIMTSNAGARDINKPMIGFGDQKVSSAAIKEAVEKIFTPEFRNRLDAIIPFNSLDEK
                     TIRYITKNEIKKLNERLFEKNVEIRLSDDCIHYLAKEGYSAEFGARNISRTIDEKISE
                     PLVEEVLFGKLEKGGIAHCSIKDGKIEIEYEAKAKKRK"
     gene            complement(20858..21169)
                     /locus_tag="PKW26_03205"
     CDS             complement(20858..21169)
                     /locus_tag="PKW26_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOQ92609.1"
                     /translation="MELETSKIVETVNKNKTKLLEPKNYRVILYNDNFTTKEFVVEVL
                     MRRFNKKHNEALILMEKVHNQNQAIIGSYPYDIAATRMSLTIQDARQNGFPLRCEMEE
                     G"
     gene            21316..22092
                     /locus_tag="PKW26_03210"
     CDS             21316..22092
                     /locus_tag="PKW26_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOQ92610.1"
                     /translation="MKKILAWNVNGIRAIEKKGFIPWLFDSGADVLCIQETKAKPEQL
                     SDDLLKPHLNGEGYFSYFSSALRPGYSGTAIYSKKEPDAVFTMGVSEFDEEGRTLAAQ
                     FGSTVVISAYFPNSQEAGARLDYKLAYCDAMLAFCDDLVKKGNNIILCGDYNIAHKEI
                     DLANPKANEKNPGYLPEERAWMDLFTSSGYCDLFRKFNTEPHNYTWWSYRFKAREKNI
                     GWRIDYQCVNETFVPAVSSSTILADQMGSDHCPIAIEVDF"
     gene            22092..22718
                     /locus_tag="PKW26_03215"
     CDS             22092..22718
                     /locus_tag="PKW26_03215"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOQ92611.1"
                     /translation="MASLRLKKEDVGAFTQLYVLLSVIIFIVSQQSLFFASFFISPTN
                     VLSSEPFSLANPWHYLRLFFHPFGHHSVLALCANAAFLFFLSPLVEKKYSKSILLGMF
                     FTCALVSASLTVAFFQNPIQGGTGFTFLYLLLCLIDSLEKQEFSFSLCIILILFLIRV
                     FYEAVDEQDLSAFMHLVGSLVASILAFIHTKKPIRRKPVAKKENKAKK"
BASE COUNT         6334 a         4479 c         4500 g         7331 t
ORIGIN      
        1 taaggctaaa cctgctcaaa caggttccaa tgcgactaat ccacttgttt taagtgtgtc
       61 aactttggat gggaagttca gtcctttttt ctatacttct gcttatgaca atgaagtaca
      121 agaaaaaaca cagcttggac ttttgtatta taataaagaa ggagcccctg aggctggcat
      181 ggctgtaccc tgtcttgcat gggactatag ccaagatgta aatgcagatc aatctttaac
      241 tacgtatacc tttattttaa aagataagat agtctttagt gatggaaaac cagttactgc
      301 aaaagacgtt ttattttcaa tctacaccct agctgatccc ttttatgacg ggtcatctac
      361 cttttatact atgaaaattc aagggataaa tgaataccga ttgcaaacga gtgctgaagt
      421 tcttaaaaat gttgagagca ttcttgctgc tggcgttggt gtcgacggaa gtggaaaact
      481 tctaccaaat ccagtaagcg gtatttctgc tgaccaagta gaacgctttt ggtcctacgt
      541 ggatgaaggt ggtgtcaagt ttgctcaaga aatcgttgac tatgttttaa ataattatct
      601 ttcatatgtt ggttctgatt atttccctgg ctttacagct gatcaagtaa aggcaagtgc
      661 aagtttgcaa acagcctttg gtatggctat gtggggcttt ggtgaagtag ttgacggtag
      721 catctttgaa gatgcaaatg gagtcgaata taatctagat actgacaccc ttgatgcaag
      781 catttattgg gacaatattg ttggtgcata tggctatgac ctgagcgatg gcggcatcaa
      841 tgcagaaaaa gcaggcgatc tcaccgtaca agactataca aaagatgcgt atatttctgc
      901 tgaaggacaa attgaaggcg gcgtaaaatc tattaccggt attacttctg gaaaaatgaa
      961 atgtgatgat ggtgtaacac gtgaatatat ccaagtggta attgacggtg tggatccaaa
     1021 agctattttt aacttaggta ttcttgttgc tcctatgcat tattatacag ctggatttaa
     1081 aggaaagctc aatgagtttg gtgtagcaac cgcaaaccgt gaatttattg aagtattaaa
     1141 atcaaaaaat agtaatcctg tgggtgctgg tccctatatt ttcaaggaat acaaggactc
     1201 tgtcgttact tataccgcaa atgacaaatt cttacttggt gcaccaaaaa ttaaaaccct
     1261 acgtttaaaa gagattgctc ttggttctga acttgattct gtgcttactg gagaagtaca
     1321 ttttaccgat ccatctgcac aaaactcaat tatcaacaac atttctactg gcgatggtaa
     1381 ttatgcaaac cttgcatata ccctcgttga taatgacggt tatggctata tcggtataaa
     1441 tgcacaagct gttcctgagt ttgaaattcg ccgagcgctt gctcatgcta tgaacgtgca
     1501 acttgctgtt gacaactact accaagaact tgcaagtgta aactatagaa caatgacaaa
     1561 gattctttgg gcatatccag ataatccaac aaatcttttc ccttatgatg gaacaggtga
     1621 aacttccaag gctcttttct tagaagccgg ttatacctat gatgcagcaa aaaatatcat
     1681 gagctatcct gcagggcatg aaaaagcggg tcaacaagtt acctataagt ttacgcttcc
     1741 ttcaaatgcc tctgatcacc cagctggttc aatttatatc gatacgcaag acgtactttc
     1801 aaaaattggt gttaaggtaa atattgacgt agatcaaaac cttttgggaa aacttgatac
     1861 agcctatgag tcaggcgttg ttgtttgggc agctgcatgg ggctctggtg gcgtcgatcc
     1921 agatatgttc caaatatggc actcagatcc aaaacaaaac caaagtacct ctcctgttgc
     1981 aaagggcctg tactggatgt ttcaaaatgg ttctgatgaa gaaaaagcta tgcttaacga
     2041 actcaatgat ttgattattg ctggtcgctc aacactcgat gtggaagaaa gaaaggctat
     2101 ctatggccgc gctctcgacc tttctactgg tttagctgtt gaaattccaa cctatcaaag
     2161 aaagaactta tatgtatata acaaaaaaat tgtaaaagct tcatcactct ttagtggtaa
     2221 cgatgtaact ccatttcaat ctccaataag cttcatttgg aatgttgaat taaattaatt
     2281 gtagcaaaaa aagtgtaaga acagcgcaat ctgttcttac acttttttca taatgaggat
     2341 cttatgtgga aatatttgct gaaaagaatt tcgcttgcac ttttaatttt ggtgggcgtt
     2401 tcagcactca tctatctctt ggttatgctg atgccagcag actatattga caatcaaact
     2461 tctgctgctt tacagtctgg cgcaatgaca aaagacgatg ttttacgtct taaagaactc
     2521 tacggtttag ccgacaagag tttttttggt attacaaaga gctatggaaa atggctcggt
     2581 agtagtttaa gtggcgactt tggtttttca tttgtatacg gaaaaccagt aacccaagtt
     2641 ttaacaagct atatgtggat atcatttctc atagctttaa ttgcctttgt tttacaaatg
     2701 attattggca tacccatggg cataaaggca gcggtaaatc agtacggtgc ctatgattat
     2761 acggtatcta tatttgcaat gattggaacg gccctgcctt catttttttt ggccgctctt
     2821 ttaatgaatg tttttgcaat tcaattaaat attctgcctt tacaaggctt aactacagca
     2881 acaaaaatct ttgctgaaac agcacatcta caaatctttt tcgataagct tttacatctc
     2941 atcctaccca tcactgtttt aaccttactc ggtgttggtg gtttgatgaa gcttactcgt
     3001 accaatatgc ttgaagtgct taattcagat tatattcgaa cagctcgcgc aaaaggctta
     3061 tcggaacatg ttgtaactta taagcatgcg tttagaaata caatgattcc acttgtaacg
     3121 acgctctcgg gaatgcttcc gagtctcttc ggtggagcga tgataacaga aacagttttt
     3181 gctatcccag gaattggcta tatggcgcta cgtgcaacgc agcagggcga catacccttt
     3241 attatgggct acaatatgtt tttggcaata ttgactgttc ttggaacact aatttcagat
     3301 tttatgtaca tggtggtaga tccccgtgtt aaattgtcct aggtgggaaa tagatggcag
     3361 aagatactaa agtaacaaaa aaacaagagt cagctgaaga gatgcatttt caagttattt
     3421 ctcccgggag aatggttgca aagcgctttt tcaaaagcta cctttctata attgggcttg
     3481 caatgatagc ctttgtcttt attttttctt ttttgggtcc cgtcatagtc gatatgacat
     3541 ggaaatataa agaaatacag gtttttaaaa ttgaccgacc aaatgattta atcctcacag
     3601 cagattttat tggagcagat ggaaaaacct atacctacta tgatcgttca caaaccatag
     3661 tgcagtttaa agcacctctt tctaaacagc actggcttgg cacagatacc tctggttttg
     3721 atatttttgt acggcttatg tacggcggta ggatttcgct cacgatttct tttatcgtta
     3781 tatttttgga aacctttata ggtgttattc ttggggggat agcaggattt ttcggaaaat
     3841 gggcagatat gattataatg cgtattgtag atattttttc ttgtcttcct gcgcttccta
     3901 ttttactcgt tctttcggca actatttcat cgattgaaag tattccaaca gaacatagaa
     3961 tttattatct catggctttt ttaaccctaa tgggttggac gggagtcgca cgtattgttc
     4021 gtgggcaaat tctcatgctt agggaacaag aatacatgat ggctgctgaa acaactggta
     4081 taaaaacttt taataaaata tttaagcatc tcgttccaaa tactataccg cagctcgttg
     4141 tatctgcaac gcttggttta ggtggcatta ttttatacga gtctacctta tcctatctcg
     4201 gattgggcgt tccttttcct cgagctgcct ggggttcaat gatagccctt gcagatccgt
     4261 caaaggggca agagattctt gcaatgtatc ctaatatgtg ggttccagca ggaattctta
     4321 ttgtggtaac tgttttaggt tttagcttta ttggtgatgg cttacgagat gcctttgacc
     4381 cacgaatgaa acgctaggaa tagcgagata aggaaaaaaa gaatgggctt ttttaatttt
     4441 aaaaataaaa aaatacagct agaagatgat atcgatgaaa caatgtattt gagtgtaaaa
     4501 gaagagcgac gcattgcaaa agaaaacaaa aaagtctttc gtgattacac taaactcaaa
     4561 cgcagaaaaa atgtttccga ggaagaatat cttacacaaa tgcgcgatag tgcaaatgta
     4621 gtagaattcg atgacttaca tacatatttt tataccgatg ttggtaccgt aaagtccgtc
     4681 aacggtgtta gtttcgatgt gcctcaaggg aagattgtcg gagttgtggg cgagtctggt
     4741 tgtggaaaat cggtaacgag tttgtcctta atgcaattgg tgcaagctcc aacgggacaa
     4801 attgtaagcg gaagtattcg ctatcaagat gctacaggaa agtgttatga aataacagat
     4861 atgcccacag atgcaatgct tcatattcgt ggaaaagata ttgcaatgat tttccaagaa
     4921 ccaatgacaa gtctaaatcc tgtttttaaa ataggcaatc aaatggatga ggttgtattg
     4981 cttcatgttg aagatgcaac tagtgaatct gcaaaagcgc gttcaataga aatgctgaac
     5041 ctcgtaggca tcgccgatgt agaaaaagta tatgatagct acccgcatga actttctggt
     5101 ggtatgagac agcgtgtaat gattgcgatg gcgctttctt gcgacccgcg ccttattata
     5161 gccgatgagc caacaacagc gctcgatgtt acgatacaag cacaaatcct tgaattgctt
     5221 cgtgaggtaa aagatagaat ccacggaagt attatgctta tcacccatga cctcggtgtt
     5281 atcgctgaga tggcggactt agttgtggtt atgtatgcgg gacgaattgt agaaaaaggc
     5341 acggtgcaag aaattttcaa aaatccaaaa catccctata ctatcgggct tatgaaaagt
     5401 agacctggca gtcaaaatac aggcgatcgt ttatatagca taaagggaca ggttcccaat
     5461 ccaatcaata tgccgccata ttgttatttt tataatcgtt gcgataaggc ctttgctccc
     5521 tgttctcaat cttatccaga attgatgcag ttttcaaaaa cgcattttgc aaactgtcat
     5581 ctctacgacc caaaatatgc atcaggcttg aaagatgaaa aaagggagaa acaaaatgga
     5641 taaaaataat actgctaaaa atcaggatgc aaatactata ctgacagtaa aaaacttaaa
     5701 aaaatattat ccactttcaa atattgaatt tggtagacaa aaaaaatttg ttaaggctgt
     5761 cgatgatgtt tcttttgcta ttcaaaaagg aacaacaatg ggcttggttg gtgaatccgg
     5821 ttgtggaaaa acaaccgttg ggcgaacaat tttacgctta catgaagtaa cttctggtca
     5881 agtttttttt gacggaaaag acttggcaaa aatttcttct cgacaattgc gtttgctccg
     5941 tcctcaaata cagatgattt tccaagaccc ctattcaagt ctttctcccc gtttacctgt
     6001 tggtgaaatt ataggggagg cggtaaaaga gcataagatt gttcccaaaa gcgaataccg
     6061 ttcttatatc cataaggtta tgagtgactg cggtttacaa agccaccatt actatcgcta
     6121 cccccatgag ttttcaggtg ggcagcgaca gagaattagt attgcacggg cagttgcctt
     6181 gcaaccaaaa tttataattt gtgatgagcc tttaagtgcg ctcgatgttt ctattcaagc
     6241 acaaattata aatcttatga aagacttaca agaccaatac ggatacacct atttgtttat
     6301 ttcccatgac ctttcagttg tggaatatat ttcagataca attggtgtta tgtatttggg
     6361 aaaactcgta gaagttgcaa gcaaaaaaca attgtttgaa aaccccatgc atccctattc
     6421 tgaagcgctt ttgtcggctg ttcccgtagc aaaccccgat ataaaaatga atcgcatcat
     6481 tctaaaggga gatattccaa gcccggtcaa tccaccatct ggttgttcct ttcgcacgcg
     6541 ctgcccgaag gtgatgccag tctgtgctga aaaagaaccg cttttgaaag attacggcga
     6601 agggcattgc cttgcttgtc atctctatgc tcaagattag gagtttgttt tttgaaagaa
     6661 agtcgaaaag aaacaataga taaaaaggca aatagtctaa acgatgacgg aagggttttt
     6721 gctgacatga gcatcgatgg aatgcctcct tcactttttg gtggtataaa aaaagccttc
     6781 ggtgtttgtg gagggaaaag atgtaaggag caaaaaaaaa caaactccga aaaatctttt
     6841 tttaagctag atagagaaga aaaaaaacag cgccttgcaa ttagccttgg ggtcttttct
     6901 tcctatcttg tttttactct tgtttttttt ggcattattg ctttgttttt tttgtttagt
     6961 caatttattt ggtttaaata aagctcatgg agagatacag cttttttaag atggaggaaa
     7021 aaatgaaagg aaattctttt tataaaaata gatttgtgta tatatccctt gttttacttt
     7081 taatctttgc tttaagttct tgcgggaaaa aaataagaat tgttgatcca gttgaattgg
     7141 gtttttcttg cacagtctat tacaatgcac tcggcggaac agtcaataaa cgcgagatac
     7201 gagaaaccta ctatgagcca ggctcttttc tttttatgcc ttcaggtaca agcaatatgc
     7261 tgatcgaacc tattcgagaa ggctatattc tcgcaggctg gtataaggca aaaactgata
     7321 tcctcgatga aaatggaaaa gtaattgcct atgattttaa ggctgaagat cgttgggact
     7381 ttgcagagga tcgagtctac gacgatatga gtttatatgc aagatggatt ccccaaggaa
     7441 aggctgagta tatagatgcg gaaactggtc ttgttatgtt tagcaaaaat atatctgaca
     7501 aatcgcccgt gcttgctttg acaagggctg cggaaaatct tataaagaaa aaaggctata
     7561 cctttgcagg ctattttacc aatccagatt ttacgaagcc ctttgatttt tctgctcgcc
     7621 aaatcaatgc tttgaagcca aacgaaaaag actttgaaaa agaagttacc agtctttacc
     7681 cgcaaataaa tctagaaaaa ctttctgaaa gcgaaaaaat acttgttcgt actgttaaaa
     7741 acaatctcta cgaagcttat atccaagaat atattgaaaa cacaaagagt caaaatattt
     7801 tcctcaagta tgaaaagggt cttgttatac atgttgcgag tttggaagac ttacgctata
     7861 aggggcagtt gagtttctcc ggacttaccg ttgatggcga gcccgttgat cgctatagta
     7921 tagaaaaaga tattgatttt aaaggagtga gtttagttat gggagagtct ttttctggga
     7981 aaatttctgg aaacggacat agcctaaaaa atatttctct cgtacttaat agtaaaccga
     8041 tcgataaaga caaagagaaa aagctttcac tttttgaaaa cctagaagat gcaattattg
     8101 aagatttgca tatagaaaat tttgtaatta aaatcaatgc aaatgcaggt gtaaaagtta
     8161 tggctgcacc cttggctatc aatggaaaaa atctaagtct aaaaaatgtc tccctacaaa
     8221 atatacagat cgatacagga cgctccgatg atggtagcgc agaatatctt ttaggtgatt
     8281 tatttgtaaa ctctgaaaat atatcccttg caaatacgaa aggatctcag tttgttttta
     8341 gtcattcatc ttttgcaaaa gtgcatagac tgcttacaag ataagatgct ttaaaaataa
     8401 aagaggctgt ctccttcgac agcctctttt ttttagcagc ttgtttattc ttcctcaaaa
     8461 aaacccattg attgaatttt tttatttcga tagttcacaa gaacagatgg atttcgtttc
     8521 attaaatcct ttaagtcttt taggatttgt tgtttaatat tgtttgctgt ttcttgtgga
     8581 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntctcgtaa gtttagggca
     8701 atagcttctc caatgccgcg ttcttctgcg ccaagcccgg gataagctcc ctgtgtatct
     8761 atgaaggtga taatcggtcg gttaaatttc tccgcttgtt ttgcaaggcg aagtgctttt
     8821 ctataacctt caggatttgc catgccacca tttctgtcga tggtttcttt taagttacgc
     8881 cctttttgat taccgattac cgttacagga atgccgtcga taaaacctat gccaccaacc
     8941 atagcgaggt catcgccaaa gtatcgatcg ccgtgaagtt caacaaagtc gtcgacaatc
     9001 atttctataa aatcaagggt tcgcggtctg tcgctatgtc tggcaagttc aacgtttttc
     9061 catacttttg aaactatgtc ttttgagctt tgtatttttt tagttatctc ctgtagttca
     9121 tcacgtatgt ccaagccttt ttcttttgcc aaaatttcaa ggtcttttaa taattgttca
     9181 tccttgcttt ggttcatttt tcaccccctt gactgtgtgc ttttatcatc tttgcaaaaa
     9241 aagctttttg ttcacttcga ggaacaatac aatcgacaaa acctttttgt agttgaaact
     9301 cagctgtttg aaaaccttct ggaagttttt gtctaatcgt accttcaata acgcgaggac
     9361 cagcaaagcc gataagtgct ccaggttcag cacagataat atcgccgagc atagcaaagc
     9421 ttgcagttac tccgcctgtc gttggatcgc aaagcatgat aaaaagtgga actccggcct
     9481 tgtccatttc tgctgcagca ctcgaggttt ttgccatttg catgagggag agtaagcctt
     9541 cttgcattcg ggcgcctccg cttgttgcat agataatgac aggagttctt tcacttgccc
     9601 cttttagaag caatcgtgaa attttctcgc caacaacgga gcccatcgag ccgcccataa
     9661 aatcaaagga cataagtgcg agtaaagctt tctcgccttc gatcgtacag ctcccagtga
     9721 ggaccgcttc ttttaaagca gattttgctt cggctgcttg aagtttttct tcgtagcctt
     9781 ccatttgtat aggattttta ctttttaatg ttttatataa ttctttaaaa ctatctttgt
     9841 ctgtaaggta ttctattctt tgccaaacag tcatacgcaa atggcgagaa cattcagggc
     9901 ataccatcaa gtgttgctca aagaggtcgc tgtcatgact tattctgcaa tgtggacatt
     9961 ccatagcact cataatttct cctattattc taaatacact acgagtgatt ttttcgtagt
    10021 gtattatttc ttatactgtt ttgtcaggga aaagctcggt atataagctc gttccaaact
    10081 gacctgacat aaattttttt gaagttaaaa tacttttttg ctcttcgata tttgtaggta
    10141 ctccatcgat aacaagctcg tccaaggcgc gaagcatttt tttcaaagct cgctctcggt
    10201 ttgtgtcatg gacgatgagt tttgcaatca ttgaatcgta gtgggggagt actgtatagc
    10261 cattgtataa aaagctatcg aaacgaactc ctataccacc gggcacctca agcctttgta
    10321 cctttcctgg agcccgtgca tttattctcg tttcgataga ccaacccttg agcttgtttt
    10381 tttcgctcga gacttccata ggtaaaccag agcaaacaag gatttgttct cgcacgatgt
    10441 ctgagtccgt tacaaactcg gttactggat gttccacttg aatacgcgca ttcacttcca
    10501 taaagaaaaa tgcatctcct gaaacaagaa attctatagt tccagccccg cgatatttta
    10561 agtttgcaaa aagatttttt gctccctctg tcatttcttg tcgcattttt gggctaactg
    10621 agggggaggg actttcttca attagttttt ggtgattttt ttgaactgaa caatcacgct
    10681 cgccgagcac agcgacattt cccttaccat cggctacaac ttgcagctca acgtggcgcg
    10741 gatttactaa gtatttttca atatagactg ttccgtcagc aaagtttgct tcagcctcac
    10801 gagatgcaat tgttaagttt tccttgaggt cttctttttt ataaacaatt ctcataccct
    10861 tgccgccacc accagaggca gcttttatga taacaggaaa accgcatttt tcagaaatag
    10921 caattgcttc ttcttctgtt tttatagcac catcgcttcc tggtgtaata ggaagtcctg
    10981 cttttatagc agttgcacgg gcttgcacct tatctccgag catttcaatt acatcagatt
    11041 ttggtccaat ccaaatgagg ccagcttttt cgacgtcgcg aacaaagcga ggattttcgg
    11101 aaagaaagcc aactcctgga tgtatagcct gacaaccagt ttgtaatgca atcgtaatga
    11161 gcgcttctgc gtttaagtaa cttttacttg agggtgcagg tcctatgcaa taggctttgt
    11221 cggcgagttt tacgtgtaag ttttctttgt cggcagatga atagactgcg acagtttcaa
    11281 tgcccatttc cttacaggtt cgaataacgc gaaccgcaat ttctccacgg ttcgcaataa
    11341 gtattttttt tatcatgctt ttctcttaga ttttttttac tgtaaataaa tcttgatcaa
    11401 attctactag ttcgccattt gttacaagga tgtcttcaat gatgcaatca aattcagctt
    11461 ctaggctatt catcattttc atagcttcaa gtatgcagat aacatctcct ttttttacct
    11521 tcttgccttt ttctacatag gcgggggagt cgggagaagg tgagcgataa aaggttccaa
    11581 ctataggact tttaatagta agtgcatcgc ttagcttact tgatgcagct gctttttttt
    11641 cgcttgcagc ttctgctgta ttttttgtag caacagggga ggcagctgcc tgactcgtag
    11701 ctgttggagc aaactgtaca ggagcttgtg attgtatagc tcggtcattc ttgctcaagg
    11761 aaatctttgt tccatctttt tgtatttcca attcttttac agagcttgta tcaaattttt
    11821 ctagtaagct taaaataaat gcttcgttca tgttttcctc tttttctccc gatgcataca
    11881 aacttgtaca tcggaagttt gtgtcgtatt ttaaatgcgg tcgaagcgtt ctgtatcagc
    11941 ttcgtaatct actcctgcaa tatcaaaacc tgaagttgcc aagaagtcgt gtctatatcc
    12001 ttcaaggtct gcaagttcgc ctacgttttc ttgagtaacg agtggcatga tgcgatcaac
    12061 ttcagcttga acttgtgggt ccatttccca gtcatcaatg cggattcggt tttcttcgtc
    12121 aacaggaatt tttgcaaact cagaaccatc ggtagcgagg taaagtcgtt cccaaaacaa
    12181 tcgattgatt tgttcgatgc agccttcatg gcttccttgc tctttcatta ctttgaagag
    12241 tacagaaaga tacaagggga taatggggat aactgcactg gcacgggtta ccaagccctt
    12301 attgactgaa acataggctt caccttttat gtttgccatt ttttctgtca aaacctttgc
    12361 ggttttttct aagtgttctt ttgccttacc aatagttccc cttctataaa tagcttgtga
    12421 aacttctgga ccaacataag aataggcgag agtgaggcag ttctctgcaa ggagttccgc
    12481 atcagaaagc tggtctatcc atcgctccca atcttctcca cccataactt ttactgtttg
    12541 catcgcctca tcttcgtttg caggttcagc agaaatttct ttcatttcgc cagtcattgc
    12601 atcgatggtt gttcctgtgt attttgtgcc aaatggtttt agaacagatt tatacataac
    12661 tcccgtttct ggatcggttc taacagggct tgcaagcgaa taaataacaa ggtcaaagac
    12721 catatttttt tcttttgctg cttgtataac ttcttgtttt atggggtcag aaaaagcatc
    12781 tccgtcaatt gtaacagaaa ctaagcctgc tttttttgcc tccgcatcga atgcgaggtt
    12841 gttgtaccaa cctggtgttc cgccctttgt ttctgttcct gctttttcat aggatacgcc
    12901 tatggtcgca gcatcgtagc caaaggctgc ggtaattctg cttgcaagtc cgtagccatt
    12961 tgagcaacca agcacgagta cgttttttat agggcgaaga tttttcccct ttgaagcgtt
    13021 ttttttgtca tattcagctc taactttttt tgtgatttca atttgatcta aaacagattg
    13081 cttacatcca gctggatgtg cgtttaaaca aatattgctt cgaatcattg gttttattat
    13141 catgagtttt tctcctgttt tttagtttgc gctgccaact aagcacatcc attcagcttc
    13201 tgcggcaact tcatcaccga cataggcttt tccagattgc ttaatcatct ttggcgatac
    13261 acgtaggttt tttacttcca ttcgtacctt gtcgccaggg cgaacttgtc ggcgaaattt
    13321 taccttatca acagttgcca aaaagaagag agagccttct gcaaacgttt tttgatagct
    13381 caatgccgct ccacctgctt gtgccattgt ttcgacgagg ataacgccag gaacaacggg
    13441 gtattcgggg aagtggcctt caaagaaaaa ttcatcttca gtaaaaatat gttcgcttac
    13501 tgaaccttcg gcatcgcatg aaagaatttc atcgacaaat aaaaatggtt ttctgtgcgg
    13561 gattaaactt ttaatatctt ttgtaacact catttatttt accttcttaa aaataaggac
    13621 gccattgtgc ccaccaaagc ctaaggagcc agatgctgca catgagatac caccagtggc
    13681 agaagcgtca attcccttgt ttgggacata gtcgagatcg caaccttcat ctatggctgg
    13741 atcgtccaag ttaattgttg gaggataaaa tccttcttca attgctttta ctgataaaat
    13801 agcttctata ccgccggcag caccgaggca gtgcccagtc atgctttttg ttgaagaaac
    13861 cttcatctta tatgcgtgtt caccaaaagc catttttatc atttgtgttt cggaaggatc
    13921 gttaattgga gtcgatgtcc cgtgtgcatt gtagtaatcg acgtcttcgg gttttacgcc
    13981 tgcatctttg agagcggttt ttagtgccaa agccccgcca attcctgagg ggtcaggact
    14041 tgttatatgg taggcatcgg aggaagctcc gtagcctgca agttcacaat agatttttgc
    14101 accacgcttg agggcagatt cgtattcttc taagattaag atacctgcac cttctcccat
    14161 aacaaaacct tcccttcgtt tatcaaaggg acgacttgcc tttgttgggt tatcattaaa
    14221 ggaagtgggt aaggtttgta aaacgctaaa gccaccgata ccaaaaccgg ttaccgttgc
    14281 ctcagttccg ccagaaatac acatgtccat gcgaccgctg cgtatttggt cgagagcaag
    14341 accgagggca tcggttccag aagcacaagc ggttgcaatc gtaaagccca taccatgtaa
    14401 gccatagatc atagaaatat ttcccggtgc ttcatttgga ataagcaagg gaattgttaa
    14461 aggcggaata cgtgcatttc ctgcatcaaa gtattttttc atagaatgct ctgttatttc
    14521 aaaaccacct ataccgtttc ctaagataca acctgttttt tctttgtcga ttgtatcctt
    14581 tgtcaaacct gcatcttcta aagcttgggc tgttgcagca accgcgagct gagtgaagcg
    14641 cgccatcttt cgtgcatccc gtctgtccat ccaatccgat gggttaaagt cttttacttc
    14701 acctgcaatc ttacaactaa aatcgctcgc atcaaattgt gtaatttttg taataccact
    14761 ttttccttct tttacgccag accaggtttc tgccaaagta ttccccaagg gagtgacagc
    14821 acctaagcct gttacgacaa ctcgtctcat cttaggcccc catgccacca tcaacgccga
    14881 taatttgcgc tgtaatatag ctcgataaat ctgacgctaa aaagacacag gagtcagcaa
    14941 catttttcat ggttccaccc tttttcattg gaattgtctc aagccatttt tctcgagctt
    15001 cttctggaat agcttgagtc atatcagtat cgataaaacc aggtgctatg agatttactc
    15061 gcactccgcg acctgcaact tcttttgcaa gactcttgct atagccgatg agtccagcct
    15121 tgctcgctga atagtttact tgtccgcctt gaccatggag tccgacgata ctagacatat
    15181 tgataattga accttttcgc ttttttatca tatctgatga aataacttgg ctaatgataa
    15241 acacgcttgt taggtttacg cgaataacat cgtcccagtc cttcatagac attctaaatg
    15301 ataagccatc tcttgttata cccgcattat ttaccagaat gtcaaagcct ccagaatctt
    15361 tgagtgcctt ttccaaaaca ggaacaactt cctctttgtt agaaacgtcg gcataaattt
    15421 ctgtaaagct tccgccaaaa gattttgcat acgcttcgag ctcttctttt actggtgacg
    15481 gtttaattga tacaccccat acgcttgcgc cttcttccaa atatcgctta acgatttcta
    15541 caccgatacc ttgataggta cctgtaacta atgctgtttt tccttttaat aacatacttt
    15601 acctcactta gataattgat tgtatttgtt caagagttcc acaagggata ctcgtaattg
    15661 cttctgcata gccagaatct cgccataaac cagataaaac ttttcccggt ccaacttcaa
    15721 gtaagactgc ctttgacttt gactcttcaa aaaaagcatg aatctttttt tctacacttg
    15781 tccataaaac aggataggta atatgttcta ccgcacgtct ttttgcttct tctccgcttt
    15841 tgattatatc accggtaaca ttggaaaaaa agagtgtttt gggattttga aaagaaattg
    15901 tttctaaaaa gtttccaaag tccttggcag ccttgtgcat gagcggggag tgaaagggcc
    15961 cggcgacttg taaacgtata aatcgccttg cacctttttc tttacagagc ctttcacaga
    16021 cttccaagcc ttcagccgtt cctgcaacca ctgtttgttt ggggctgttc atgttgacgg
    16081 gaaaaacaat gcccttttct ccagaatatt cttttagaat tgctattatt tcatctgttt
    16141 ctaagccaat aaccgccgcc attccagggc ttgcattgtc aagctcgctg cttgaacttt
    16201 cattttgaat tgcttcgcaa gcctcctgca tgattctccc tcgctctttt acaactcgaa
    16261 tggtatcagc aaaggatagg acaccgcttg tgtagagggc agggtactcg cccaagctaa
    16321 aaccagcgac ggcatcagcc tttataccct ttatttcaag gacctttgca atggcaaggg
    16381 aagcaactgt aatcgcaagc tgactgcgat cgctacgtga aagaagactc gcatcggagt
    16441 tccaaagcca ttcgcttaaa gcttcgccag aaatctcttc ggcttctttt acaagccgca
    16501 tcgcttcagg atgggcgtcg caaatgtcct ttaccattcc aggaaattgt gcaccctgtc
    16561 caggaaataa aaaaactgta ctcattaaaa cctcttttta ttaaaacgct tgtttttttt
    16621 accttttatt tctcgtgttt tttttctatt attctattat ttaataggta gaatatttaa
    16681 tagatagaaa aaattaccag cgaaaaacag ctccagcata ggtaagacct gcaccgaagg
    16741 cagctgaaac aatagtcatg ccttctttaa gcattccttt ttcgtccata tcgacgaggg
    16801 tgagaggaat agaggcagaa gacgtgtttc catattcatc aaggctgata ataaatttat
    16861 ccattttaaa accgaggcgt tttgccgcag cacttaaaat gcgctcattt gcttggtggc
    16921 agacaaagta gtcgaccata tcttcagtta gattttcttt ttccatcaag tctttaatga
    16981 gttgtgtcat aatgccgacg gcaaaattgt atacagcgcg accgtccatt tgtaatgctt
    17041 ctccaaattt attgtagaga tattgacttc ctgaaccttg agctccgagg ataaaactgg
    17101 aaagacctct ttctgcttta tctatttctg cattattttc caagagaata gttccagcac
    17161 catcgccaaa aagaacgcag gtcgctctgt cttcccagtc tactatagaa gaaagcacct
    17221 ctgttccaat aatgagtgcg tatttccaat tatgttttgc cattaaagct tcggctgtgt
    17281 cgagggcata taaaaagccc gaacaagctg ctgataagtc aaagcaggct gcgttttcag
    17341 cagcaaggct ttcttgaatg aggcaggcat tggcagggaa gccgtgatag tcgggagtcg
    17401 cagttgcaca aacaataagg tcaacttgat ttgcttcgag acccgctttt ttgagggctt
    17461 cttctgaaga gcgtgcacct aagcttgcac tcgtttcgcc ttcctcagcg atatatcgag
    17521 cgccaatgcc agtgtgtgag cgaatccatt catcgcttgt atcaattttt tttgctaaat
    17581 catcgtttat tacgtgattt tttgggagag ccttcgcggc tgctctaata attacagaca
    17641 ttttttctcc ttatttctca ttatgacaaa aagctcataa atgtcaatgg ttagttagac
    17701 gaaagtgcga aaaatgtcat aatgtcaaat cgtatacata ttagacaagg agaggacaaa
    17761 aaggttgcaa aagtctttgc tacgaaagtt tttatgagaa gtgattttga aagtgctcaa
    17821 tcactgagct tttaggtttt gtaaagagaa ggtctttttc aagtcgtaaa aaagccgatc
    17881 ggctaatatt tttccctcca aagcgagcta tatggtcagt gtagatctgt gaatcaatga
    17941 cttctccgcc taaatcaaaa aaaatctggg caaattcagc aaagatcgcc ttggaagctt
    18001 ctggttttaa ggtaaacatt gactccccaa aaaaaatctt tcctataagc acgccgtaaa
    18061 agccgccgac gagttttcca gcctgccaag tttcaaaaga atgtgcataa ccaagtctgt
    18121 gaaattctgt ataggcttgg ataacttcgt cgattatcca tgtatgtttt tgatccacgc
    18181 gttttacatg ggcgcagttt gtaattactg cttcgaagtc tgtatttacc gtgtaataga
    18241 aggcagttgg gtctttttgg ctttgttttt ctttattttg ctttataaaa cgtttgaggc
    18301 gtttgggaat atgaaaatct ttttttaaga tagtaaaacg agggtcggga gaccaccaca
    18361 aaataggttc atcggcatta taccagggaa aaatgccctg taaataggct gagagaagca
    18421 tgccagggga gagatttccg tccatacaga caaccccgtc ctgcgtttcg tttggatcgg
    18481 ggaaggggaa gaaaaaatct ataggaaggt agggaaaatt attgctattt tcgttttttt
    18541 gcttttgcct catattctat ctctattttt ccatctttga tgctacaatg agcaatgcca
    18601 cctttttcaa gcttgccaaa aagtacttct tcaacgaggg gctcagagat tttttcgtcg
    18661 atagtgcgcg aaatatttcg agcgccaaat tctgccgaat aaccttcctt cgccaagtag
    18721 tgaatgcagt catcgctgag gcgtatttca acattttttt caaaaagccg ttcgtttagt
    18781 ttttttattt catttttggt gatatatcta atggtttttt cgtctaagct gttgaagggg
    18841 ataattgcat cgagcctatt tcgaaattct ggagtaaata ttttttcaac tgcttctttg
    18901 attgccgctg aagaaacctt ttggtctcca aaaccaatca tgggcttgtt aatatcacgc
    18961 gcgcccgcat tactcgtcat aataataatc gaggagcgga agtcagcttt ttttccgtgg
    19021 ttgtcggtga gcgaggcgta gtccattatt tgcaagagca ggttgtaaat atcgggatgt
    19081 gctttttcaa tttcatcaaa taaaataagc gcatgaggat tttttcgaac cgcatcgctt
    19141 aaaagccctc cgtcttcgga gccgacataa cccggaggcg agccaataag acggcttacg
    19201 gtatgttttt cttgatattc ggacatatcg aagcgtaaaa agctaatatt gagtagttca
    19261 gcaagttttt ttgcaagttc ggtttttcca actccagttg gaccggcaaa taaaaagttt
    19321 gcgattggtt tttctggtgc acggaagcct gcgcgagagc gttttaccga ttttgttata
    19381 gcttcaatag cttcgttttg tccaaagatt tcttttttta aatctgaatc caagcttttt
    19441 aagcttgttt tttcatcgga tgagaccgtt ttttcgggta tcctcgttat ttttgaaagt
    19501 gttttttcta ttaattgttt agtaactttt tgatcctttg ttgggtgtaa gcgtaggtag
    19561 gcgccagcct catcgatgag gtcgattgcc ttgtcgggga ggtgccgttc agtgatatag
    19621 agttttgaaa gtacaactgc ggcttcaagt gcttcatcag tatactgaac ctggtgatgt
    19681 gtttcgtatc gttcttgtaa accttttaga atttctatag tctcagcttc tgttggctct
    19741 ataatgtcaa ttttttgaaa gcgccgtgca agagctctgt ctttttcaaa ggtttttgcg
    19801 tattcttcat gggttgtcga accaatacag cgaaaggtgt tttttgcaag gaggggtttt
    19861 aaaaggtttg acgcatcaag accgctactt gcagaggagc cagcaccaaa aataccatgt
    19921 acctcatcta taaataaaat agtagactgc tctttaagaa gctcttctcc cagttttttt
    19981 attctatctt caaaatcgcc tcggaacttt gtacctgcga tgagggctgt catatcgaga
    20041 ctgagaattt taaatttttt gagaaaatct ggtacttctc cagaaacgat tctttgcgcc
    20101 agtccctctg tgattgctgt tttccctgtg ccggcatcgc cgacatgaac gggattgttc
    20161 tttgtgcgtc gacataagat ttgtatcgtt cgctctattt cctcatctct tcctataagt
    20221 gtatcgagtt ttccttcttt tgcttcttcg ttgaggtttg tcgtaaagcg ttcgagaaag
    20281 gttttcttta ccttttcgtt ttgctttgct ttttgttttt tattttgctt gttaagttcg
    20341 tcgagcttct ccaaatctgc aattaaggca gactcaacac cattttcaat atctgtaata
    20401 ttttcattta catcgacacc agagttttct aacatgtcga gataggtgat attttcaagt
    20461 aagttgaggc gagatacccc agagtttttt agaaaataac tacaatggtt gtttttctca
    20521 tcgtagagac ttacaagcac atcggtatat tcaatgcttt ttttttctgc agaggcacaa
    20581 tgtaaaagag ttcggtcaat aatggacgaa aacccaattg tttgcacggg atccgcttgc
    20641 cctttgtttt ctgcaacagg aactcttgtt tttaaatatt catcaacact tcgtgttaaa
    20701 taggggaggt ctgccccaca ccaatcgaga aactccaaga tttgtttatt gtctaaaaaa
    20761 actttcagaa ggtgttctgg tgttacaaac tcatgctgtt gtttttttgc ttcattaaag
    20821 gcgtttttta ttatgttttg caccaattga cttattttca tccttcctcc atttcgcaac
    20881 gtaatggaaa accattttgg cgagcgtctt gtatcgttaa agacattcgc gttgctgcta
    20941 tatcataggg ataactccca atgatagcct gattttggtt atgtactttt tccatgagga
    21001 tgagagcctc attgtgtttt ttattaaatc gtctcataag gacttcaact acaaattctt
    21061 ttgttgtgaa attatcattg tagagaataa cacgataatt cttcggttct aagagcttcg
    21121 ttttattttt attgacagtt tcaactatct tgctcgtttc taattccata aaaaaagcac
    21181 tccctatata tagtataact cataatgcaa agatttcatt ctaaatctct ttaaaaatac
    21241 acaaaaatac aggtaaaatc aagtttttat cttctaagac ttgaaaaaaa acgataaaaa
    21301 agggaaacta agcctatgaa aaagatttta gcttggaatg taaatggaat tcgtgctatt
    21361 gaaaaaaaag gctttattcc ctggcttttt gatagtggtg cagatgttct ttgtatacaa
    21421 gaaacaaagg caaaacctga acagctttca gatgatttgt taaaaccaca tttgaatgga
    21481 gaagggtatt ttagttattt ttccagcgct ctgcgaccag gttattctgg aacagcaatt
    21541 tatagcaaaa aagaacccga tgctgttttt acgatgggag tgagcgaatt cgatgaagag
    21601 gggcgaacct tggcagcgca gttcggtagc acagttgtaa tatcagcata ttttccaaac
    21661 agtcaagagg caggagcccg gctcgattat aaacttgctt attgtgatgc aatgttggca
    21721 ttttgcgatg accttgtaaa aaagggaaat aatattattc tttgtgggga ttataatatt
    21781 gcccataagg aaatagactt ggcaaatcca aaggcaaatg aaaaaaatcc cggctatcta
    21841 cccgaagagc gtgcgtggat ggatcttttt acgagttcag gctattgcga tttgtttcga
    21901 aaattcaata cagaaccgca taattatacc tggtggtcgt atcgatttaa agcacgtgaa
    21961 aaaaatatcg gctggcgtat cgattaccaa tgtgtcaacg aaacttttgt tccagctgtt
    22021 tcttcgagta ctattctcgc tgatcaaatg ggtagcgatc attgccccat tgcaatcgag
    22081 gttgattttt aatggcatca ttgagactga aaaaagagga tgtaggtgct tttactcagc
    22141 tttatgtgtt gctttctgta attattttta ttgtatcaca gcagtcttta ttttttgctt
    22201 ctttttttat aagtcctaca aatgtcttga gctccgagcc cttttcgctt gctaatccct
    22261 ggcattatct ccgtctcttt tttcatccct tcgggcatca ttcggtttta gccctttgtg
    22321 caaacgccgc ctttcttttt tttctctctc cccttgttga aaaaaaatac tcaaaatcta
    22381 ttttactggg aatgtttttt acatgcgccc tcgtttcggc aagtttgaca gtggcttttt
    22441 ttcaaaatcc tattcagggt ggaactggtt ttactttttt atatctttta ctctgtctca
    22501 tcgattctct tgaaaaacaa gaattctctt tttcactttg tattatttta atactttttc
    22561 taatacgcgt gttttatgaa gcggttgatg aacaagacct ttctgccttc atgcacttag
    22621 tcggctctct cgtagcaagc attttagctt ttattcatac aaaaaaaccg ataaggcgaa
    22681 aacctgtagc aaaaaaagaa aacaaggcaa aaaaatgagc cttgataaag agagaaaaag
    22741 cgcc
//
LOCUS       DAOSSW010000021        21854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_2581_length_21854_cov_7.254002, whole genome shotgun sequence.
ACCESSION   DAOSSW010000021 DAOSSW010000000
VERSION     DAOSSW010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 21854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21854
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2581_length_21854_cov_7.254002"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PKW26_03220"
     CDS             <1..578
                     /locus_tag="PKW26_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOQ92612.1"
                     /translation="VTKNDELAQRMKTMRLHGIDRPVWDRYSSPKASWLYDVVEAGYK
                     YNLPDILAAIGREQLKKVFFLYEQRKEIVDSYNAAFAHCNWIIPPPDGIGNAWHLYFL
                     QLDLEKLTIDRDSFALELQENGVSVSVHFIPHYHMSYWKKRGLEVKDFPESEKKFNST
                     LSLPLWPGMTKKMANFVIDRVKEIGEKAYVR"
     gene            568..2637
                     /locus_tag="PKW26_03225"
     CDS             568..2637
                     /locus_tag="PKW26_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOQ92613.1"
                     /translation="MCAKTQKKQFEFINSYYSDLSLDEQVIALIVRSPVASGTIRSIN
                     HPSLPEGYSIFTKKDLRWETDIEVLDTKIPLLAYEKISYEGEAIAIVCGPSIHVARRI
                     IAGLDIQFTEALEEAKNPEEDIVLHDIFTRAIDMSQNKKKIGSEIESLKDETIYTINK
                     TYSTKIKHEFYKESIGCLAHFSKKNLSIFTPSLWAQNLVENVSAVTNIATEFISIKKT
                     ISHHLSESNIWAESYIACLASYASLLLEKPVFLQIEKKDEIPFFYNSPEFSTSFNVSF
                     TSEGKILSIDAHCQIDCGAFPIFKEEIINRTKVACIGAYSIPEQKIRVDILKSAKPPL
                     SIDINAVDTHAQFAIEVLMQDIAMKLKLFPHELRLKNIETAQSGILDLGAEEIPSLIE
                     TSIDKSQFLRRYATYNLVSQDPELFLKHKPCRGIGMSIGFQGSSFASSILENKRKELS
                     LTLRTDESVIIHAHTHSKKIANIWKQMAAQELSIPPEKIKLDPSYAVLSDAFPSTIHR
                     NINITTSLIKRCAKGIEAKRFHEALPIVEKKAIYTQRPKTWNLKKLEGKPYFSYAWAS
                     AVIDLEIDSYSYEVLIKKIWVTIHAGKILNREDAEKSVKKNIQQQLSRYLPEESYVSF
                     LIDLHFISSEDDPKEIGSLMNNVIPSALHSALTLALQKPIPQLPLVHDSVFKLIKGKE
                     NENTMHS"
     gene            2615..3112
                     /locus_tag="PKW26_03230"
     CDS             2615..3112
                     /locus_tag="PKW26_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOQ92614.1"
                     /translation="MKIQCILNGKLTLIETEVQDLLSDSLRKNGLLSVKNGCYQGKCG
                     ACTILLNKRPVPSCLVYTSSLNNDEVETLEYFYKSEDYQDIEEAFVILGIKLCGFCNA
                     GTLFTLYDILQRSKQFQKKELETKIRTINCDCVEEESLLKAVLLAKQIKDRRLGEEIK
                     KYGKR"
     gene            3099..3968
                     /locus_tag="PKW26_03235"
     CDS             3099..3968
                     /locus_tag="PKW26_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOQ92615.1"
                     /translation="MEKDKLDFEKNIQVISAQNLEELFYFQKSVRDLYIVGAGTTKEH
                     LSNSHKGIKSILFTRTITDLCQIEKKERYIDIGSASSLAQILELGEKIIPKVFYDALK
                     SIANPFIKNLATIGGNICYTKKRLTLFSPLLALEAGLEFKASASDTLYIPISKFHEVP
                     KGYFLSKIRIPIEDWDISIFKKLGPQETINAFSAFFTFLAQIEKTVVVDVRIAFCGNI
                     SFRHKELENKLLGIRLPLSNKFKNELILEAEELYNLRFEADQKKNKIDLDSDALLKQQ
                     YLNLLSFSLDYLS"
     gene            complement(3970..5022)
                     /gene="queA"
                     /locus_tag="PKW26_03240"
     CDS             complement(3970..5022)
                     /gene="queA"
                     /locus_tag="PKW26_03240"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOQ92616.1"
                     /translation="MKTSDFYFNLPEDCIAQYPSKIRGDDRLLVLNRDKDIDDSALFV
                     HKKMSDFLSLVDENSLLVFNNSRVRKSRLFAKKINSSQTVEFLFIHQYADGSWKVMVR
                     NAKKHKPGHGYVFSDGTEAKIIERESDRGTEFRSLLFDVAVDEAWFERLGHVPLPPYI
                     KREDALFDESRYQNIYASEVGSSACPTAGLHFTPDILEALKNKGIDTAFLTLHVGLGT
                     FLPVRSEKIEEHTMHEEVYTISEETADKINAAKKEGKKIIAVGTTSVRSLESNWDDKK
                     KCLTPGSFATSIFLYPGYEFKLVDQLFTNFHTPESTLLMLVSAFAGKEKIFKAYDEAI
                     KEKYRFFSYGDAMFIR"
     gene            complement(4997..5995)
                     /gene="ruvB"
                     /locus_tag="PKW26_03245"
     CDS             complement(4997..5995)
                     /gene="ruvB"
                     /locus_tag="PKW26_03245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOQ92617.1"
                     /translation="MHLPEDSQDNSLRPLSLNEFMGQSKIKENLSIFIEAAVSRKESL
                     DHLFLIGPPGLGKTSLAHITACELGVDFKVTSAPALDKPKDLVGILSTITERTVFFID
                     EIHRLKPAIEEMLYMAMEDFELDFVIGQGPQARTVRIPIPHFTLVGATTKAGNVSSPL
                     ISRFGIVERFSYYSDEELSRIIKRSSAILSIQIDTEAALLLASCARGTPRVANRLLRR
                     MRDFAHVAGKDIIDTELVQDSLKRLEIDALGLEKHDRDILKTIIQQYDGGPVGAETLS
                     ISIGESVDTLEDYYEPYLIQAGLIQRTSRGRIVTAKAYAHLGLEGKPPRENERLLF"
     gene            complement(6091..6681)
                     /gene="ruvA"
                     /locus_tag="PKW26_03250"
     CDS             complement(6091..6681)
                     /gene="ruvA"
                     /locus_tag="PKW26_03250"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOQ92618.1"
                     /translation="MIHSLSGYISQKLAQNLLLENAGIEWQIEMPDTDIASLPAVGEK
                     VRIFTHLHHKEDSMRLFGFASFESRETFFDLLKVNGIGPKAAIKILSSIHYEQLIEAL
                     DTENMAVLEKISGVGKVTAQKMVLALKGKIRFISEKETEAREEWADLVQALADMGYDK
                     KDARLVLKKAALRLDSSLSQSQKEEALLKAAILELN"
     gene            complement(6775..6847)
                     /locus_tag="PKW26_03255"
     tRNA            complement(6775..6847)
                     /locus_tag="PKW26_03255"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6812..6814),aa:Val,seq:tac)
     gene            6996..8282
                     /locus_tag="PKW26_03260"
     CDS             6996..8282
                     /locus_tag="PKW26_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOQ92619.1"
                     /translation="MEKLFKLNEHGTNVRKEVIAGITTFLTMAYILAVNPSMLAETGM
                     SAGGVFTATVVASAIATLVMAFLANLPVALAPGMGLNAFFTYTVVLGMGVSWQVALTA
                     VLLEGLLFIVLSFFNVREAIINAIPSNLKKAVAVGIGLFIALIGLANTNLVVSDAGTI
                     IGLGALEGAALLAVIGLVITIILYALKVPGSILIGIIITTLIGIPMGVTNIPHNFKIL
                     SLPEKPIFMQFDFSSVLTLKFFTIFFTFLFVDIFDTVGTLVGVTTQAGLTDKDGNIPK
                     VKQALLADAVGTVAGAALGTSTVTSYVESTAGVAAGGRTGLTSVVTAGLFLLALFLSP
                     LFLIVPSAATAPALIFVGFLMMKSVVGINFEDPAEGIPAFICIIVMPFAYSIAEGIVW
                     GLIAYVLLKAVTGKFKDISIMSWVLFVIFIARFFIK"
     gene            complement(8370..9782)
                     /gene="thrC"
                     /locus_tag="PKW26_03265"
     CDS             complement(8370..9782)
                     /gene="thrC"
                     /locus_tag="PKW26_03265"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOQ92620.1"
                     /translation="MMYTDTRELNTRVSFRTAVLNGMNEKSGGLYVPIEYPFLEKQFL
                     NKNPEPSFRDIAFEAAKLFVGDEIPDNNLQEIIIDAYPFTAQTVPIDPTTYVLELHHG
                     PTCAFKDFGARFMARTMSYFNRDEDDVLHILVATSGDTGSAVGSAFQDVPGIDVTILY
                     PKGKISPLQEKQLSTFTGNVRSLAVDGTFDDCQKLVKTAFTDSDLRNNLRLSSANSIN
                     ISRLIPQSFYYLYSSLVVRNRAPRDSRIDNPAIIFVVPSGNFGNLTSGLIAQKMGAPI
                     SGFVAATNANRTVPDWIATGNYKPRPSIATYSNAMDVGAPSNYERIINMYNLDEVRSL
                     FASYWLDDAGTLQAIKNCHTRTGYMLDPHGAVAWQAWDDMRRGGMEALLRGEDFDYTK
                     PGLTKNIPSWAQKIIENKAVGVLLETAHPAKFGDIVQSAIGREPVMPDRLEKVLYLPN
                     TALSMENNYEVFKSWLLENL"
     gene            10050..10613
                     /locus_tag="PKW26_03270"
     CDS             10050..10613
                     /locus_tag="PKW26_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOQ92621.1"
                     /translation="MNSMFERLGALLKERLEGDEQDIFKDSKKINTEKSFADEEIKKE
                     DFSQKMQTEKASHSEEKIEEKLGEKAKANTKEKSKTSTYSQGKGHFSFDRSKQDQSYH
                     KKAYTIYKAESLKKPMPKAIQRDFDFLALSYSASLEECKERYKKLIKAYHPDKHSSDL
                     RKQKNATEHSAKINAAFNRIRTWFETN"
     gene            complement(10627..11496)
                     /locus_tag="PKW26_03275"
     CDS             complement(10627..11496)
                     /locus_tag="PKW26_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOQ92622.1"
                     /translation="MKSMTAYAIVEKQTAKNALSIEIKSYNSRYLDISIILPSFLNRL
                     EAELKHLVHKTISRGKIEVFVRYKEMDPQIKVEINEPMISAYDEAFSALAKKMGRKKE
                     KLPMSFLLSLDSVLSNEKTIDIDLAKESLEAVLQEALELCVADKKREGAHLQKELLAQ
                     ISLLEESVHIFEKNQKKMEAQLKQSIKERYAELVESSIPEDKLMSELALLLMKYTIQE
                     EIVRLKSHIEILKKEMLENSQPGKRIDFICQEIQREVNTIGSKNQISEVSQSVVQAKE
                     ALENIREQAKNIE"
     gene            complement(11493..13007)
                     /gene="murC"
                     /locus_tag="PKW26_03280"
     CDS             complement(11493..13007)
                     /gene="murC"
                     /locus_tag="PKW26_03280"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOQ92623.1"
                     /translation="MHNSNLSFEKKLPRDMSGIRLFFTGIKGTGMTALVEICHERGAI
                     IDGSDIDDVFYTDAILSSLDIRPKLFSKNNITENIDLVFHSSAYDPESHEELLEAKAL
                     GIPIMEYTIALGEISKASFSVAVSGVHGKTSTTSIIGTILKELPLESQVLVGSQVPSF
                     GNSCTMHKGSEFFIAETCEYKKHFLHFHPSIILMTSVEEDHQDFFPHYEDMRDAFVEF
                     VSLLPENGRLIYCADDKGSRDVVSIVKQKRKDIRYIAYGKNAKDAGETYFYTFTNLYL
                     ESGTQNFTVQTPDFEKKLSLRVPGYHSVLNSLGAIAVCLSLWEEKGNSLNEAILDSIG
                     KALLQFVGAKRRSEIVADIPQSPSNPYGALIIDDYAHHPTAIETTLKGFRDFYPDRLL
                     IVSFMSHTYSRTHALLDAFSKAFSSADIVILHKIYSSARENKEKYIGNTSGEILFQKT
                     SAHHPQVLYFDEFLDAQDEVLHLMQNKASVFITMGAGDNWKLGLAVKNRLEDTK"
     gene            complement(13085..15754)
                     /gene="ppdK"
                     /locus_tag="PKW26_03285"
     CDS             complement(13085..15754)
                     /gene="ppdK"
                     /locus_tag="PKW26_03285"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOQ92624.1"
                     /translation="MAKTQLVYFFGGGKAEGDASMKTLLGGKGANLAEMTNLSIPVPP
                     GFTIATEVCKLYYDNNKKHPVGLKEEVQANLEKLEKLTGKKLGDPDDPLLVSIRSGAA
                     MSMPGMMDTVLNLGMNDKAVQGLINKTGKDRFAWDSYRRFIQMFGDVAMGVPHAAFEK
                     ALEDMKAKKKVVLDSDLSAADLEALVGEYKKIVKKHAKEDFPQDPLKQLWFSIDAVLN
                     SWNNDRAIKYRALNNIKGLAGTAVNVQAMVFGNFGETSGTGVCFSRDPSTGENIFYGE
                     YLMNAQGEDVVAGIRTPEKISTLEKENKKVYDQLVKIKNNLEKHYKDMQDMEFTVQEG
                     ELFILQTRSGKRTGQAAVKCAVDMVNERLIKKEEAVMRVTPDQIDQLLHPMIDPKVIK
                     TTKALTKGLNASPGAACGQIVYTADEADQWVKEGKKVVLVRKETSPEDITGMVVAEGI
                     LTSTGGMTSHAAVVARGMGTPCVCGATEVQFIGGAVLINGTKLKKGDFITIDGTTGAV
                     YKDSLPLIDVKVSKELDTFLEWCDEVRNKAVRKTEHLTLKGFGIRANADQGEDAKTAF
                     DFGAEGIGLCRTEHMFFDKEKIIHVRAMIVSDTEAQRKAALKKIMPLQKKDFLAIFKA
                     MKGKPVTIRLLDPPLHEFIPQNKAEIEELAELMNLKVAEVKAKLDSLHELNPMLGHRG
                     CRLAITYPEIYATQVEAITLAAIEMKKKKLDVKPEIMIPLICEPQELEIIRRDSEALI
                     KELLDKAKIKLDIQIGTMIEVPRAALLAHKIAEHADFFSFGTNDLTQTAFAFSRDDAG
                     KFLPDYVEKGLFEADPFKTLDESGVGELVSIAVERGRSTNKGIKLGICGEHGGDPQTI
                     DFCYRVGLDYVSCSPYRVPIARLAAAQAVLNNA"
     gene            15853..17055
                     /locus_tag="PKW26_03290"
     CDS             15853..17055
                     /locus_tag="PKW26_03290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOQ92625.1"
                     /translation="MKTHKLNIFLVLFTTFMLFYACKTIDSIKVEKTQGIDSLKEEIP
                     EEVFVVDTFIPEEKTLRLSFSGDLMAHINVTRMKDFSLIWKDVKPLFEEEGLHFINLE
                     TPVHDKRPYENYPSFNVKKAYVEAALDAGFNVFSLANNHTNDQGKEGILETEKYFAEL
                     EKRDVFWAGIKKEADENFSYALIEKNGFKILFFAYTEILNQEAHRELIDYVRPNKKDR
                     DVFINFAKKIKAETMADFCVISVHCAEPEYVLEVPASRKIFYHSLTEELADIVWANHP
                     HLVQEWEWIGRLDTGRIEKAIFYACGNTISGQRLSLNYEYPAAMREYTGDGIVFSLDL
                     KKDAEEKTYIESYSHQLITTHIDEDRNSLVQFLDLDFIKNQNEKNKKYYEKRLELMNK
                     IQGTITWK"
     gene            17046..19601
                     /locus_tag="PKW26_03295"
     CDS             17046..19601
                     /locus_tag="PKW26_03295"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA];
                     GO_process: GO:0008380 - RNA splicing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
                     /protein_id="HOQ92626.1"
                     /translation="MEITRPKPNYKELPVYAQKSKILDAIQNNQVIIIQSPTGSGKTT
                     QIPVILHEAGFSENGIIGVTQPRRIAALSVSEFIAKQLQTNFPGLVGYKMRFEDKTDL
                     STKIKIMTDGILLQEMKLDPYLSKYSVIIIDEAHERSLNIDFILGLLKRILRMRKDFK
                     VIVSSATMNAEAFSVYFEGCPVISIDTETWPVDLIYDPLPQSTTQSAAFTDMLLEKVE
                     SIVYRIFDNKHKGDILIFLPGEKIIKDLTSHLYRSALSKELFIIPLYGRLSKEEQEKV
                     FYPTPPDKTKVVIATNIAETSITIDGITTVIDSGLAKINYYNPQTFTSSLNETLISKA
                     SCNQRRGRAGRTQAGVCYRLYSRNDYDRRSLYTLEEIYRTDLSEVVLRMSELGITDFE
                     SFDFIAPPNPLGLESAIETLFLLGALEKDYSLSAIGKHMVEFPLMPRHSRIIVEAMVR
                     YPSVMEEVLIATAFLSAQSPFILPQGEEMDARRAHHRFRNPDGDFLSYIHIFRLFTEA
                     KNQELFCEKSYLDHRVMSEIVNIKIQLEQIVSDLGFPILSGGSRLDYLTSVASGLIQF
                     ICFNHGRGMYRSFTQENIQIHPGSNMFKEEPAIFVAGEVFKTTKMFAMSVSPISKEIL
                     NRLNIKMPTKQVRGKEIYEVKKSQSQKGRQREAQDVERQKQMRDENIVRIGPESFELK
                     KIKGKKILNLPLTKFSPLVQDERYENRISQLADLRGCLLINYKSLLSGEKISLIAKLV
                     KELNLSPLPQIDAIRNKNFYYNENPGRLIDSLSYVLRVTEARAKTKELGFITLYSNDI
                     GNYWYKISRGFSTALNESLASVENLIAKEDVELNSEQKTILNATYRKLSSLYT"
     gene            complement(19638..20807)
                     /gene="hemW"
                     /locus_tag="PKW26_03300"
     CDS             complement(19638..20807)
                     /gene="hemW"
                     /locus_tag="PKW26_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOQ92627.1"
                     /translation="MEVALYIHIPFCASKCSYCDFYSLACRDIPAWYIDRLEEEFFFR
                     IKQFNIKKIKSLYFGGGTPSYLLQNPVLKQALSSFFSKIKPFLRPDAECSFECNPDDI
                     SEEACIFLKSLGINRISLGIQSFNNTKLAFLNRRSSKAINEKALSIIQKAGFSHFSAD
                     LISGLAPLGKNFKTEATELYEAIDKLISFGVDHISLYSLIVSPGTLLEKQLEENPNLF
                     DEALSEKLWIGGRDYLEKKGFMQYEVSNFSLPGCESQHNLCYWDMETYLGLGAGATGS
                     LFLPDGKTLRYENTEDLEAYKNRDFLESQETECIEKKDAIFEYLMMGFRKNAGISASV
                     FSKRFSESIESYIEPHFSNWEKKSLAKKRQEGSEIYYALTKKGLLFLNQFLEALL"
     gene            complement(20811..21557)
                     /gene="lepB"
                     /locus_tag="PKW26_03305"
     CDS             complement(20811..21557)
                     /gene="lepB"
                     /locus_tag="PKW26_03305"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOQ92628.1"
                     /translation="MINNKSVQNPYELSYIMKRQLQKRISTLVFFVVFTIAAIFFVKT
                     FLLFSFIPQTNAVFSVTENKDRVFVSPFLKPLNTLIPSSMKISHGDLVLVNTSKTKDI
                     GQIKKITKAIVSFFTLNKIELFTEHPRAFSNSSLLRVLACPGDSLYIKDFIVYVKDKN
                     SQFFLTEFEMSKKDYEIQKELGQVDESHPLYIALGNMETIVLAENEYFLLSDNRLANL
                     DSRLWGPVDAKNIKGKALFRVQLFKDASFL"
     gene            21680..21751
                     /locus_tag="PKW26_03310"
     tRNA            21680..21751
                     /locus_tag="PKW26_03310"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21713..21715,aa:Gly,seq:tcc)
BASE COUNT         6843 a         4284 c         4084 g         6643 t
ORIGIN      
        1 tcgttacaaa aaatgacgag cttgcacagc gtatgaaaac aatgcgcctg cacggtatag
       61 accgccccgt ttgggataga tatagttcac caaaagcttc ctggctctat gatgttgtag
      121 aagcgggcta taaatataat ctgcctgaca ttctagcagc aataggaagg gagcaactaa
      181 aaaaagtttt ttttctatat gaacaaagaa aagaaattgt tgatagttac aatgcagcat
      241 ttgctcactg taattggatt attcccccac cggatggaat aggaaacgcc tggcatttat
      301 attttttaca gttggattta gaaaaactaa ctatagatcg cgatagcttt gccttagaat
      361 tgcaagaaaa tggggtgagc gtttccgttc attttatacc gcattatcat atgagctact
      421 ggaaaaaaag aggcttagaa gtaaaagact ttccagaaag tgaaaaaaaa tttaactcga
      481 cgctttcact accactttgg ccagggatga caaaaaaaat ggcaaacttt gttatagata
      541 gggtaaaaga aattggggag aaggcctatg tgcgctaaga ctcaaaaaaa acaatttgag
      601 tttattaatt cctactatag cgatttaagt cttgatgaac aagtgattgc actcattgta
      661 cgaagcccag tagcaagcgg aactattcgc tctatcaatc atccctcgct gcctgaagga
      721 tatagtatct ttacaaaaaa agatttacgt tgggaaacag atatagaggt actcgataca
      781 aaaatccccc ttcttgccta cgaaaaaatt tcatacgaag gtgaagctat cgcaattgtt
      841 tgtggaccct ctattcatgt agcaaggcgc attattgcag gcttagacat tcaatttaca
      901 gaggctcttg aagaggcaaa aaaccctgag gaggatatcg tcttacacga tatatttaca
      961 cgcgccatcg atatgagtca aaataaaaag aaaataggtt ctgaaataga atcgctaaaa
     1021 gacgaaacaa tctatacgat aaacaaaacc tattcaacaa agataaagca cgaattctat
     1081 aaagaaagta tcggctgtct tgctcatttt tcaaaaaaaa atctaagtat ttttacgcca
     1141 agtctatggg cacaaaacct agtcgaaaat gtttctgcgg taacaaatat cgcaacagaa
     1201 ttcatttcta taaaaaaaac catatcgcat catctcagtg aatcaaatat ttgggcagaa
     1261 agctatattg cctgccttgc aagctatgca tcgcttcttt tagaaaagcc tgttttttta
     1321 caaatagaaa aaaaagatga aattcctttt ttttacaata gcccagaatt ttcaacatct
     1381 ttcaatgtct cctttacctc tgaaggaaaa atactatcga ttgacgcaca ttgccaaatt
     1441 gattgtggcg cctttcctat ttttaaagaa gaaatcataa atagaacaaa ggttgcttgc
     1501 ataggcgcat atagtatccc agaacaaaag atacgcgtcg atattttaaa aagtgcaaaa
     1561 ccaccccttt caatagatat aaatgctgtc gatacacatg cacaatttgc aatcgaagtg
     1621 cttatgcaag acattgctat gaagttaaaa ctttttcctc atgaactgcg cttaaaaaat
     1681 attgaaaccg ctcaaagcgg gattcttgat ttaggtgccg aggaaatacc ttctctcatt
     1741 gaaacatcca tagataaatc acaattcctt cgccgttacg caacctataa tcttgtttcg
     1801 caggacccag agctcttttt aaaacataaa ccctgccgag gaattggtat gagtattggt
     1861 tttcaaggaa gtagttttgc ttcatcaatc ctcgaaaata aaagaaaaga gctttccctc
     1921 actttaagaa cagatgaatc tgttataata cacgcccata ctcattctaa aaaaattgca
     1981 aatatttgga aacaaatggc tgcacaagag ctttctattc ctcctgaaaa aataaaactt
     2041 gacccttctt atgcggtttt aagtgatgcc ttcccttcta caattcatag aaatatcaat
     2101 ataacgacat ccctcataaa aagatgcgcg aagggtatag aagcaaaacg ttttcacgaa
     2161 gccttaccta tcgtagaaaa aaaagctatt tatacgcaaa gacctaaaac atggaactta
     2221 aaaaaactag aaggaaagcc gtatttttct tatgcatggg cttcagcggt aatagattta
     2281 gaaatagata gctatagtta tgaagtttta ataaaaaaaa tatgggttac aattcatgcc
     2341 ggaaaaatat taaatcgaga agatgccgaa aaatcagtaa agaaaaatat acagcagcag
     2401 ctatctcgct accttcccga ggaaagctat gtaagctttt tgatagatct gcactttata
     2461 agctctgagg atgaccccaa agaaataggt tctttgatga acaatgttat tccttccgcg
     2521 cttcattccg cccttaccct tgcattgcaa aagcccattc cacaacttcc cctcgtccat
     2581 gacagcgtat ttaaattgat taaaggaaaa gaaaatgaaa atacaatgca ttcttaatgg
     2641 aaagctaacc ttaatcgaaa cagaagttca agatctttta agcgatagct tgcgtaaaaa
     2701 tggcttacta tcggttaaaa acggttgtta tcaggggaaa tgtggtgcct gtactatttt
     2761 actcaataaa agacctgttc cctcttgctt ggtgtacacc tcctccctca ataacgatga
     2821 agttgaaacg ctcgaatatt tttacaagtc tgaggattat caagatatag aagaagcatt
     2881 tgtcatattg ggcataaaac tctgtggatt ttgtaacgcg ggtacccttt ttacactcta
     2941 tgatatattg caaagatcga agcagtttca aaaaaaagaa ttagaaacaa aaattagaac
     3001 catcaattgt gattgtgtgg aagaagaaag tttattgaag gcggtccttc ttgcaaaaca
     3061 aataaaagat agaagacttg gcgaggaaat aaaaaaatat ggaaaaagat aagttagatt
     3121 ttgaaaaaaa catacaagtt ataagcgcac aaaatcttga agagcttttt tattttcaaa
     3181 agtcagtacg agatctatat attgttggtg cgggaacaac aaaagaacat ttaagcaatt
     3241 ctcacaaagg cataaaatca atcctcttta caagaactat tactgaccta tgccaaatag
     3301 aaaaaaaaga acgctacatc gatattggct cagcaagctc tctcgctcaa atcctagagc
     3361 tcggtgaaaa aattattccc aaggtatttt atgatgcact taaaagtatc gcaaacccat
     3421 ttataaaaaa tcttgctaca attggtggaa atatttgtta tacgaaaaaa agactcaccc
     3481 ttttttcacc gctcctcgcc cttgaagcag gcctagaatt taaagcgtcc gcttctgata
     3541 ccctgtacat acctatttca aaatttcatg aagttcccaa gggctatttt ctttcaaaaa
     3601 ttcgaatccc catcgaagac tgggatataa gtatttttaa aaaacttggt ccacaagaaa
     3661 caataaatgc tttttcagct ttttttacat ttctcgcaca aatagaaaaa accgttgtcg
     3721 tcgatgttcg tatagccttt tgtggcaata ttagttttag gcataaagaa ttggaaaata
     3781 aattactagg gatacgcttg cctttaagta acaagtttaa aaacgaactc atacttgaag
     3841 cagaagaact ttacaacctt cgctttgaag cagatcagaa aaaaaataag atagacctag
     3901 atagcgatgc cttgttgaaa cagcagtatc tcaacttgct ttctttttca ctcgattatc
     3961 tcagctagat tagcgaataa acattgcatc tccgtaagaa aaaaaacgat acttttcttt
     4021 tattgcctca tcgtaggctt taaaaatttt ttcctttcct gcaaaggcag aaacaagcat
     4081 gagtaaggtt gactctgggg tgtgaaagtt ggtaaacaat tggtcaacaa gtttaaactc
     4141 atagccagga tataaaaaaa tagaggttgc aaagcttcct ggagtaaggc attttttttt
     4201 atcatcccag tttgactcaa ggcttcgaac agaggtagtg ccgactgcaa tgattttttt
     4261 tccttctttt tttgcggcat ttattttatc tgccgtctct tcactgatag tataaacctc
     4321 ttcgtgcatt gtatgctctt cgattttttc agagcgaaca ggtaaaaaag taccgagtcc
     4381 cacatgaaga gtgagaaaag ctgtatctat tcctttgttt tttaaagctt ctaaaatatc
     4441 gggcgtaaaa tgtaaaccag cagttggaca ggcagaagaa ccaacctcac ttgcataaat
     4501 attttgatag cggctttcat caaacaaggc atcctcgcgt ttaatatagg gcggaagtgg
     4561 tacgtgaccg agcctttcaa accaggcttc atctaccgct acatcaaaga ggaggctacg
     4621 aaactctgtt cccctgtcgc tctcccgctc gataattttt gcttcagtcc catctgaaaa
     4681 aacatagcca tgccctggct tgtgtttttt tgcattgcga accattacct tccaagatcc
     4741 atctgcatac tggtgaataa ataaaaactc tacagtctga gaagaattta ttttttttgc
     4801 aaagagccga gattttctca cccgtgaatt attaaaaact aaaagggagt tttcatcgac
     4861 caatgaaaga aagtcagaca tttttttatg tacaaaaagt gccgagtcat ctatgtcctt
     4921 atcacgattt aaaacaagga gtctatcatc tcctcgtatt tttgaagggt attgcgcaat
     4981 acagtcttcg ggtaaattaa aataaaagtc gctcgttttc acgcggtggt tttccttcta
     5041 agcctaaatg tgcataggcc tttgcagtta caatacgccc tcgacttgtc ctttggatta
     5101 agcctgcttg tatgagatag ggttcgtaat aatcttccaa ggtatcaacc gactccccta
     5161 tcgatatgct aagagtttca gcaccgacgg gtcccccatc gtattgctgg ataattgttt
     5221 ttaaaatatc tctatcatgt ttttctaaac caagggcatc gatttcaaga cgttttaagg
     5281 aatcctgcac aagctcagta tcgattatat ctttcccggc tacgtgagca aaatccctca
     5341 tacgtcgcag gagcctgttt gcaacacgtg gcgttcctcg tgcacaagaa gcaagcaaaa
     5401 gagcagcttc tgtatcaatt tgtatcgata ggatagcgct cgatcgtttt ataatacgag
     5461 agagttcttc gtcgctataa tatgaaaaac gctccactat gccaaatcgg ctgataagtg
     5521 ggctcgacac attcccagcc ttcgttgtcg ctcctacaag cgtaaaatgc ggtatgggaa
     5581 tgcgcaccgt ccgtgcttgg ggtccctgcc ctatgacaaa gtctagctca aaatcttcca
     5641 ttgccatata aagcatctct tcaatggcag gttttaagcg atgaatttca tctatgaaaa
     5701 ataccgttcg ctccgtaatt gtcgataaga taccgaccaa gtccttgggt ttatccaagg
     5761 caggtgcact tgttacttta aaatcaaccc ctaactcgca agcggtgata tgtgcaaggc
     5821 ttgtctttcc caaaccaggt ggaccgatta aaaaaagatg atcgagactt tcctttcgag
     5881 aaactgcagc ttctataaaa atagataaat tttctttaat cttcgactgt cccataaatt
     5941 catttaaact caaggggcga agtgagttat cttgagaatc ttcgggtaag tgcatgtctt
     6001 ggcggataaa tgaagttgat agattgggga agtgttcctt tgcagaatcg tagagatagt
     6061 ttttttcatc tcgatttaat atatccatat ctagttaagc tccaaaatag cagcctttaa
     6121 gagagcctct tctttttggc tttgacttaa agaagaatcg agcctaagtg cagctttttt
     6181 taagacaagg cgagcgtctt ttttatcata acccatatca gctaaggctt gcactaagtc
     6241 agcccattct tccctagcct ctgtttcttt ttcggagata aaacgtatct ttcctttcaa
     6301 ggcaagcacc attttttggg ctgtgacttt gccaacccca gaaatttttt ccagtacagc
     6361 catattttct gtgtcgagtg cttcgataag ttgttcataa tgaatgcttg aaagaatttt
     6421 tatagcagcc tttggaccta tgccatttac cttgagtaag tcaaaaaaag tttcgcgcga
     6481 ctcaaaagat gcaaaaccaa aaagtcgcat agaatcttcc ttatgatgta agtgcgtaaa
     6541 aattcttacc ttttcaccaa ccgcgggaag cgatgcaata tctgtgtcag gcatttctat
     6601 ctgccattcg atacctgcat tttccaaaag gagattttga gctagttttt gacttatata
     6661 tcctgaaaga ctgtgaatca tttgtttatt ttacttgaaa aaagctgaag gtgcaagagt
     6721 tttgagaagg tcttttaaaa tgaaaaaacc atttggaaaa atccaaatgg tttttgggcg
     6781 aagaggggat cgaacccccg acaacctggg tgtaaaccag gtgctctccc agctgagcta
     6841 ttcgcccttc gatttgttta tattagcaat atcaaaaaaa aaagtcaagt agtttagccg
     6901 ttttttcttg ttttattgaa cacaaaatga tatactacat ctttcttaca taactaaaaa
     6961 gtaagacgac catctatgga ataatggagg atagtatgga aaagttgttc aagctcaatg
     7021 agcacggaac aaatgtacgc aaagaggtta ttgcaggtat aacaaccttt ttgacaatgg
     7081 catacatatt ggcggtaaat cccagtatgc tcgcagaaac gggtatgagt gcaggtggtg
     7141 tttttaccgc taccgtcgtt gcctcagcaa ttgcaacctt ggttatggca tttttagcga
     7201 atcttccggt agcccttgca ccaggtatgg ggctcaatgc ctttttcacc tacactgttg
     7261 ttttgggaat gggtgtctca tggcaagttg ccttgaccgc agttttactc gaaggtcttt
     7321 tatttattgt cctttcgttt ttcaacgtca gggaagcaat aattaatgca attccttcca
     7381 acctgaaaaa agctgttgct gttggtatag gtttatttat tgcgcttatt ggtcttgcaa
     7441 acacaaatct cgttgtcagt gatgccggta caatcatcgg attaggtgct ttagaaggag
     7501 ctgcactact tgcagtaatt ggtcttgtta ttaccattat tttatatgca ctaaaagttc
     7561 caggctctat cctcattggt attattataa cgaccctaat cggtattcca atgggtgtta
     7621 caaatattcc tcataatttt aagattttaa gccttcctga aaaaccaatt tttatgcaat
     7681 ttgatttttc atcggtttta acattgaaat tttttactat cttttttacc ttcctctttg
     7741 tcgatatttt tgacacagta ggaaccctcg ttggtgttac cacgcaggca ggtttaaccg
     7801 ataaagacgg aaatattcct aaagtaaaac aagcgcttct tgcagacgca gttggaacag
     7861 tagctggcgc agccttaggt acatcaactg ttacaagtta tgttgaaagt accgctgggg
     7921 ttgcagcagg aggccgaact ggcttaactt ctgtagtaac cgcaggactc ttcttgctcg
     7981 ctctcttttt atccccctta ttcctcatcg tcccttcagc agcaaccgcc ccagccctta
     8041 tttttgttgg ctttttgatg atgaaatctg tagttggcat caattttgaa gatccagccg
     8101 aaggtattcc cgcctttatt tgtataatcg ttatgccctt tgcctacagc attgcagaag
     8161 gtattgtatg gggattgatc gcctacgtgc tcttaaaagc tgttaccgga aaattcaaag
     8221 atatatctat catgtcctgg gttttatttg taattttcat tgcacgcttc tttatcaaat
     8281 aagaaagtct ctgaagatgt atgcttaaat agacaaagct gcctcttttg ggcagctttt
     8341 ttgtttgtgt tttttaagtc tagataggtc tagagatttt ctaagagcca ggacttaaag
     8401 acttcgtaat tattttccat ggaaagagct gtatttggca aatacaatac tttttctaaa
     8461 cgatctggca taacaggttc tctcccaatg gcactttgca ctatatcgcc aaattttgca
     8521 gggtgtgcag tttcaagcaa aacaccaaca gccttatttt cgatgatttt ttgtgcccat
     8581 gaaggaatat ttttcgtaag acctggttta gtatagtcaa aatcctcccc acgcaataag
     8641 gcttccatac cgccacgacg catatcatcc caggcttgcc aggccacagc cccgtgcggg
     8701 tcgagcatat aaccagttct cgtatggcaa ttttttatag cctgcaaggt acctgcatcg
     8761 tcgagccagt aggaagcgaa aagagagcgt acctcatcga gattatacat attgataatg
     8821 cgctcataat tactcggtgc gccaacatcc attgcattcg aataggttgc gattgaaggt
     8881 cgaggtttat aatttccggt tgctatccaa tcggggacag ttcggtttgc atttgttgct
     8941 gccacaaaac cacttatagg agcacccatt ttttgtgcaa taagaccaga agtgagattt
     9001 ccaaaattgc cagagggaac tacaaagata attgcagggt tgtcgatacg gctatctcga
     9061 ggtgcacggt tacgcacaac caaagatgag tagagataat aaaaactttg cggaataagc
     9121 ctcgaaatat tgatagaatt tgcactggac agtcgcaagt tatttcgtaa atctgaatca
     9181 gtaaaggctg tttttaccag tttttgacaa tcatcgaagg taccatcgac agcaagagag
     9241 cgtacatttc cagtaaaagt agacaattgc ttttcttgta aaggagaaat tttacctttt
     9301 gggtagagaa tcgttacatc aattccggga acatcttgga aggcactgcc cacggcacta
     9361 cccgtatcac cagaagttgc aacgagaata tgcaaaacgt cgtcttcgtc tctattaaaa
     9421 taagacatag tccgagccat aaagcgagca ccgaaatctt taaaagcaca ggttggtcca
     9481 tggtgcagtt ctaacacata ggtcgtaggg tcgattggta ccgtttgtgc agtaaaagga
     9541 taggcatcga ttataatttc ttgtaaatta ttgtcgggta tttcatcacc cacaaaaagc
     9601 tttgcagctt caaaagcaat atctcgaaaa gaaggctcag ggtttttatt caaaaactgc
     9661 ttttcaagaa aaggatattc gataggcaca tatagcccac ctgatttttc attcatgcca
     9721 tttaatacag cggttcgaaa gctaactctg gtgtttagct ctcttgtatc ggtatacatc
     9781 atttttaaaa tcctgctttt agttattatt tgtagtatac aatggaaagc taaaaatagc
     9841 aagaattatt tattgtcgat ttttggggaa aatagcacca taatagggtc tgtttcaggg
     9901 tcaagttcgt ttccggtgaa tttataggct aagaattcgg agcctgtgtt acttgtcttt
     9961 tctcaaaaaa taaatgttaa aaaaatagta tcatgtaata aaaaaatccc tagattgata
    10021 caatgactct ttctcgctat actgggtcta tgaatagtat gtttgaacgg cttggggctc
    10081 ttttaaaaga aaggcttgaa ggtgatgaac aagacatttt taaagactca aaaaaaataa
    10141 atactgaaaa aagttttgca gatgaagaga taaaaaaaga agatttttct caaaaaatgc
    10201 agactgagaa agcttctcat agcgaagaaa aaatcgagga aaagctaggg gaaaaagcca
    10261 aagcgaatac caaagaaaaa agcaaaacat ctacatactc tcaaggcaag ggacattttt
    10321 catttgacag aagtaaacaa gaccagagct atcataaaaa agcttacaca atatataagg
    10381 cagaaagtct taaaaagccc atgcctaagg ctatacaaag ggactttgat tttctcgctc
    10441 tttcctactc tgctagctta gaagaatgca aggagcgtta taaaaaactc atcaaggcct
    10501 atcatccaga taaacatagc tctgatttgc gcaagcaaaa aaatgcaacg gagcatagtg
    10561 caaaaataaa tgcagccttt aatcgcattc gcacgtggtt tgaaacaaac taaaatctaa
    10621 aaaacactac tcaatgtttt ttgcttgttc tcgtatattt tcaagggctt cttttgcctg
    10681 cacaaccgac tgtgaaactt cactaatctg attttttgaa ccgattgtat tgacttctct
    10741 ttgtatttct tggcagataa aatcgattcg tttccctggt tgtgaatttt ctagcatttc
    10801 tttttttaaa atttcgatat gacttttaag ccgaactatt tcttcttgta tcgtatattt
    10861 catcaacaat agggcaagtt cagacataag cttatcttct gggatgctcg attccacgag
    10921 ttcggcatag cgctccttta tagattgctt caattgcgct tccatttttt tttgattttt
    10981 ctcaaatata tgtacgcttt cttcaagcaa ggagatttgt gcaagcaatt ctttttgtaa
    11041 atgcgcgcct tctctttttt tatctgccac acataattcc aaggcttctt gcaaaaccgc
    11101 ttcaaggctt tcttttgcta aatcaatgtc aattgttttt tcattggaga gcactgagtc
    11161 gagcgataaa agaaagctca tgggaagttt ctcttttttt cgacccattt tttttgccaa
    11221 cgcagaaaag gcctcgtcgt aggcactaat cattggctca tttatttcta cttttatctg
    11281 ggggtccatc tctttgtatc gcacaaagac ttctattttt cctctggaaa tagttttatg
    11341 cacaagatgt tttagttctg cttctaatcg attcaaaaag gaaggcagga taatcgatat
    11401 gtcgaggtaa cgcgaattat aggatttgat ttcgatagac agggcgtttt ttgctgtttg
    11461 tttttcaaca attgcatagg ctgtcatgct tttcattttg tgtcctccaa gcggtttttt
    11521 actgcaagtc cgagtttcca gttgtcccct gcgcccattg ttataaaaac gcttgccttg
    11581 ttttgcatga gatgcagcac ctcatcttgg gcatctaaaa actcatcaaa ataaagcacc
    11641 tgtggatgat gagcggatgt tttttgaaag agtatttcgc ctgaggtatt tcctatatat
    11701 ttttctttat tctctcgtgc tgagctataa atcttatgca agataacaat atccgcactc
    11761 gaaaaggctt ttgaaaaagc atcgagtaag gcatgggttc gcgaatacgt atgcgacata
    11821 aaactgacga ttaaaagacg gtcgggataa aaatctcgaa accctttgag cgttgtttct
    11881 atcgccgtgg gatgatgggc gtagtcgtcg atgataagag cgccataagg attcgatggg
    11941 ctttggggaa tatcggcgac tatctcgctt cgccgttttg caccaacaaa ttgcaacaga
    12001 gcttttccaa ttgaatcaag gatagcttca ttgagggaat ttcctttttc ttcccataag
    12061 ctcaggcaaa cagcaatcgc tccaagggaa tttaaaacac tatgatagcc aggcactcgc
    12121 aagctcagtt ttttttcaaa gtctggtgtt tgtactgtaa aattttgtgt accgctttca
    12181 agatagagat ttgtaaaggt ataaaaatat gtttcaccag catcttttgc gttttttcca
    12241 taggctatgt atcgtatatc ttttcgtttt tgcttaacaa tagaaacaac atcgcgcgaa
    12301 cccttgtcat cggcgcaata aatgagcctg ccattttctg gcagtaagga aacaaattca
    12361 acaaaggcat ctcgcatgtc ttcgtagtgt ggaaaaaaat cttgatggtc ttcttctaca
    12421 ctggtcatta aaataatcga gggatgaaaa tgtaaaaagt gttttttgta ttcgcaggtt
    12481 tctgctataa aaaattcact tcccttatgc attgtgcaag agtttccaaa agaaggtact
    12541 tgggagccaa caaggacttg cgactccaga ggaagctctt ttaaaatagt tccgattatg
    12601 cttgtggtgc ttgtttttcc atgaacgcca gaaacggcaa cgctaaaaga tgccttggag
    12661 atttctccga gcgcaatggt gtattccata atgggtatgc cgagcgcttt tgcttcgagc
    12721 agctcttcat gactttctgg gtcgtaggct gatgagtgaa aaacaaggtc tatattttct
    12781 gtaatattat tttttgaaaa aagctttggt ctaatatcaa gtgaagaaag aatagcatcg
    12841 gtataaaaaa catcgtcaat gtcgcttccg tcaataattg ctccgcgctc atggcatatt
    12901 tcaaccaagg cggtcatacc tgtcccctta atcccagtaa aaaaaaggcg aattccagac
    12961 atgtcgcgag gtagtttttt ttcaaatgag aggttcgaat tgtgcataat aaagaagtat
    13021 actcgaattg taaaataaaa aaaagaggta gccattttgc tacccccttt tttatgcctt
    13081 tattttaggc attattcaaa acagcttgtg ctgcagcaag tcgtgcaatt ggtacacgat
    13141 agggagaaca ggagacatag tctaagccta cacgatagca aaagtcgatt gtttgtgggt
    13201 cgccaccatg ctcaccacag ataccgagtt ttataccctt atttgtagag cgtcctcgtt
    13261 ctaccgcaat agaaaccaat tctcctacac cactttcatc gagggtttta aaaggatctg
    13321 cttcaaataa acctttttca acatagtcgg gaaggaattt tccagcatcg tctcggctaa
    13381 atgcaaaggc tgtttgcgta aggtcatttg tcccaaagct aaagaagtct gcatgttcag
    13441 caatcttatg agcaagaagt gctgctcgtg gaacttcaat cattgtacct atttgaatgt
    13501 ctagctttat ttttgcctta tctaaaagct ctttgatcag ggcttcgctg tcgcggcgta
    13561 tgatttcaag ctcttgtggt tcacaaataa gcggtatcat gatttcaggt tttacatcga
    13621 gctttttctt tttcatttcg atagcagcaa gggtaattgc ttcaacctgt gttgcataaa
    13681 tttcaggata ggtaattgca agacggcaac cacgatgtcc gagcatgggg tttagctcat
    13741 gtaaggaatc aagttttgcc ttcacttcag ctacttttaa gttcatcaat tctgcaagct
    13801 cttctatttc agccttattt tgtgggataa attcatgaag cggtgggtca agcaggcgaa
    13861 tcgtaactgg tttgcccttc attgccttga agatagcaag gaagtctttt ttttgtagcg
    13921 gcatgatttt tttaagagca gcctttcttt gcgcttcggt atcagaaaca atcattgcgc
    13981 gaacatggat aattttttct ttgtcaaaga acatatgctc agttcggcac agtcctatac
    14041 cttccgcacc aaagtcaaag gcggtttttg catcttcacc ttggtcagcg tttgcacgaa
    14101 taccaaaacc tttcagtgtc aagtgctctg tttttcgaac agccttgttt cgcacctcat
    14161 cacaccattc gaggaaggta tcaagttctt tggaaacctt tacatcgatg agtgggaggc
    14221 tatctttata gacagctcca gttgttccat cgatagtaat aaaatcacct tttttaagct
    14281 tggtaccgtt tataagaact gcaccgccaa taaattgtac ctcggttgca ccgcacacac
    14341 agggagttcc cataccacga gcaacaacag ctgcatggct tgtcatacca ccagtggaag
    14401 ttaaaatacc ttcagcgaca accattccag ttatatcttc agggcttgtt tctttacgaa
    14461 ccaatacaac cttcttgccc tcttttaccc actggtcagc ttcatctgct gtgtagacaa
    14521 tttgtccaca ggctgcaccg ggggaggcat tgagcccctt tgttagcgcc ttcgtcgttt
    14581 taattacctt tgggtcaatc atcggatgta agagttggtc aatttgatct ggtgtaacac
    14641 gcataaccgc ttcttctttt ttgataagtc gctcgtttac catgtcgact gcacatttta
    14701 ctgcagcttg tccagtacgt tttccacttc ttgtttgcaa aataaataat tcaccttctt
    14761 ggacagtaaa ctccatatct tgcatatctt tataatgctt ttcaagatta ttttttatct
    14821 taaccaattg gtcatagact tttttatttt ctttttcaag cgtagaaatt ttttctggtg
    14881 ttcgaatacc tgcaacaacg tcttcgcctt gcgcattcat taagtattcg ccataaaaaa
    14941 tattttcacc ggttgaaggg tcgcggctaa agcaaactcc tgttcctgaa gtttcgccaa
    15001 agtttccaaa aaccatcgcc tgcacgttta ctgcagtacc cgccaagcct ttaatattat
    15061 tgagtgcacg gtattttata gccctatcat tattccacga attcaatact gcatcaatag
    15121 aaaaccagag ttgcttgagt gggtcttgag ggaaatcttc ttttgcatgt ttttttacaa
    15181 tttttttgta ttcacctaca agtgcttcga ggtctgcagc gcttaagtcg gagtcaagta
    15241 caactttctt ttttgccttc atatcttcta aagctttttc aaaggcagca tgaggaacac
    15301 ccattgcaac gtctccaaac atttggataa aacgtctata ggaatcccag gcaaatctat
    15361 ccttgccggt tttgttgatg agcccttgca cagccttatc gttcatacca aggtttaaaa
    15421 cagtatccat cattcccggc atcgacatag cagcaccaga gcgaatagaa actaaaagcg
    15481 gatcatcagg atcgccgagt ttttttcccg taagtttttc aagcttttca aggtttgctt
    15541 gcacttcttc ttttaagccc acaggatgtt ttttattgtt atcgtaatat aacttacata
    15601 cctcagttgc tatagtaaaa ccgggaggaa caggtattga aagatttgtc atctctgcaa
    15661 ggtttgcgcc ctttccacct aagagtgttt tcattgaagc atcaccctct gctttcccac
    15721 caccaaaaaa atacaccaat tgtgttttcg ccataaaaat tggtactcct tcttcttttt
    15781 acaaagtaat ataacaatac tattttacaa tatcattgca gtttttgcaa actaaatgta
    15841 tactatttat gaatgaaaac acataaatta aatatcttcc ttgtcttatt tactactttc
    15901 atgctttttt acgcgtgtaa aacgatagat agtataaaag ttgaaaaaac gcaaggcatc
    15961 gattctttaa aagaagaaat tccagaagag gtatttgtag ttgatacatt tatcccagaa
    16021 gaaaaaacac ttcgtctcag cttttctggc gatctcatgg cgcatataaa cgtaacacgc
    16081 atgaaagatt tttctttgat ttggaaggat gtaaaaccgc tcttcgaaga agaaggtttg
    16141 cattttataa atcttgaaac acctgtgcac gataaacggc cctatgaaaa ctatccgagc
    16201 ttcaatgtaa aaaaagccta tgtcgaagca gcactcgacg cgggtttcaa tgtcttttct
    16261 cttgctaata atcataccaa cgaccaggga aaagaaggaa ttttagaaac cgaaaaatat
    16321 tttgctgaac tcgaaaaaag agatgttttt tgggcaggta taaaaaaaga ggcagacgaa
    16381 aattttagct atgcgcttat agaaaaaaat ggatttaaaa ttttattttt tgcctatacg
    16441 gaaatcctca atcaagaagc acatcgtgaa cttattgact atgtaagacc taataaaaaa
    16501 gacagagatg tttttataaa ctttgcaaaa aaaataaagg ctgaaacgat ggccgatttt
    16561 tgtgttatct ctgtacactg tgctgagccc gaatatgttt tagaagttcc tgcaagtcga
    16621 aaaatatttt atcattcgct tacagaagag cttgctgata ttgtatgggc aaatcatcca
    16681 cacctcgtgc aagaatggga atggatagga aggctggata cagggagaat tgaaaaagca
    16741 atcttctacg cctgcggaaa tacaatctct ggtcaacgtc tttcactcaa ctatgaatac
    16801 cctgcagcta tgcgagaata cacaggggat ggtattgttt ttagccttga cttaaaaaaa
    16861 gatgctgagg aaaagacata tatagaaagt tacagccatc aactaataac aacacatata
    16921 gatgaggata ggaattcttt agtacagttt ttggatttag attttattaa aaatcaaaat
    16981 gaaaaaaata aaaagtacta tgaaaaaaga ttggaactga tgaataaaat acaaggtaca
    17041 ataacatgga aataacacga ccaaaaccga attataaaga gctccccgta tatgcacaaa
    17101 aatcaaaaat actcgatgcc atacaaaaca atcaagttat tataatacaa agcccgacag
    17161 gttcgggtaa aacaacacag attcccgtaa tcttacacga ggcaggtttt tcagaaaatg
    17221 gaatcatcgg agtaacgcag cccagaagaa ttgcagcgct ctccgtttca gagtttatcg
    17281 caaaacaatt gcaaacaaac tttcccggct tagtgggcta taagatgcgc tttgaagata
    17341 aaacagattt aagcacaaag ataaaaataa tgaccgatgg tatcttgctg caagaaatga
    17401 aactcgatcc atacttgagt aaatactcag ttattatcat cgatgaggca catgaaagaa
    17461 gcttaaatat agattttatc ctgggcttac ttaaacgtat tttacgcatg agaaaagact
    17521 ttaaagtcat tgtctcttct gcaacaatga atgccgaagc tttttctgtt tattttgaag
    17581 gctgccctgt tatttcgata gatactgaaa cctggcccgt cgatctcatc tacgatcctc
    17641 tcccacaaag cacgacgcaa tctgccgcct ttaccgacat gcttttggaa aaggtggagt
    17701 ctatcgtcta tcgcattttt gacaataaac acaagggcga cattcttata tttttacccg
    17761 gagaaaaaat aataaaagac ctcacttctc acctctatcg cagcgcactt tctaaagagc
    17821 tttttattat tccactgtat gggaggcttt caaaagaaga gcaggaaaaa gtcttttacc
    17881 caactcctcc agataaaaca aaggtggtaa ttgcaacaaa tatcgcagaa acttctataa
    17941 cgatcgatgg cataactacg gttatcgatt ccgggcttgc aaaaataaat tattataatc
    18001 cgcaaacgtt tacatcgagc ttaaatgaaa cgcttatttc aaaagcttca tgcaatcaaa
    18061 gaaggggacg cgcaggcaga acgcaggcag gtgtttgcta taggctctac tcacgaaacg
    18121 attatgacag gagaagtctt tacacccttg aagaaatcta tcgaactgat ttatctgaag
    18181 ttgttcttcg catgagtgaa ctcggcataa ccgattttga aagctttgat tttatagcac
    18241 caccaaatcc ccttggttta gaaagcgcta tagaaacact ttttttactt ggagctttgg
    18301 aaaaagacta ctcgctctct gcaattggaa agcacatggt agaatttccc ctcatgccac
    18361 gacatagccg catcatcgtc gaagctatgg ttcgctatcc ctctgtcatg gaagaagtgc
    18421 ttatagcaac agctttttta tcggcacagt ctccctttat tttaccccaa ggtgaagaaa
    18481 tggatgcgcg aagagctcat catcgcttta gaaatcctga cggagatttt ttatcctata
    18541 tacatatttt ccgtttattt actgaagcaa aaaaccaaga gctcttttgc gaaaagtcct
    18601 acctcgatca tcgagtgatg agcgaaatag tcaatataaa aatacagttg gagcaaattg
    18661 taagcgacct cggttttcct attttatccg gtggaagtcg cctcgattat ttgacctctg
    18721 ttgcaagcgg tttaatacaa tttatttgct ttaatcacgg gcgcggcatg tatagaagct
    18781 ttacgcaaga aaatattcaa attcatcccg gctcaaatat gttcaaagaa gaacccgcta
    18841 tctttgttgc aggggaagtt tttaaaacaa caaaaatgtt tgcaatgtca gtttccccta
    18901 tttcaaaaga aatattgaat cgacttaata ttaaaatgcc tacaaaacaa gtacgcggaa
    18961 aagaaatcta tgaggtgaaa aaaagccaaa gtcaaaaagg aagacaaaga gaagcacagg
    19021 atgttgaaag gcaaaaacag atgcgagatg aaaacattgt tcgcattggt ccagaatcct
    19081 ttgagctaaa gaagataaag ggcaaaaaaa ttctcaatct tcctcttaca aaattttctc
    19141 ccttggttca agacgagcgc tatgaaaacc gtatttcaca acttgctgac cttcgcggct
    19201 gcctcttgat taactacaag agtcttcttt cgggagaaaa aatttcactc atagcaaaac
    19261 ttgtaaaaga actcaatctt tctccccttc cacagataga tgcaatacga aataaaaatt
    19321 tttactataa cgaaaatcct ggaaggctca tagatagcct ttcatatgtc ttgcgagtta
    19381 ccgaggcaag agctaaaaca aaggaactcg gttttataac gctctattca aatgacatag
    19441 gaaattattg gtataagatt tcacgcggat tttctaccgc attaaatgag agccttgcaa
    19501 gcgtcgaaaa ccttattgcc aaggaagatg tagaattaaa cagtgaacaa aaaacgattt
    19561 taaatgcaac ctatagaaag ctctcttccc tctatactta gagagcaaaa aactagaaaa
    19621 ctaaactttt gcttttctta taaaagcgct tctaaaaatt ggttgagaaa aagcaagcct
    19681 ttttttgtta gggcataata gatttcactt ccctcctgcc ttttttttgc aagggatttt
    19741 ttttcccagt ttgaaaaatg cggctcgata tagctttcga tagattcaga aaaacgctta
    19801 gaaaaaactg aggcagaaat ccctgcattt tttctaaaac ccatcatgag atattcaaaa
    19861 atcgcgtctt ttttttctat acattctgtt tcctgacttt ctaaaaaatc tctattttta
    19921 taagcttcta ggtcctcggt gttttcatag cgcagtgttt ttccatcggg caaaaaaaga
    19981 cttcccgttg ccccagcacc cagcccaaga tatgtttcca tatcccaata acaaagattg
    20041 tgctgacttt cacatccggg gagagaaaaa tttgaaacct cgtactgcat aaagcctttt
    20101 ttttctagat aatctcgccc ccctatccag agtttttctg aaagcgcttc atcaaagaga
    20161 ttgggatttt cttccaattg tttttcgagc aaggttcccg gggacacaat gagagaatac
    20221 aaggaaatat gatcgacacc aaaagaaata agcttgtcga ttgcctcgta gagttctgta
    20281 gcctcagttt taaaattttt tcccaatgga gctaggcccg aaataaggtc tgcagaaaaa
    20341 tgcgaaaagc ctgctttttg gatgatagaa agtgcttttt catttatagc cttgctagag
    20401 cgacgattta aaaaagcaag ctttgtattg ttaaaacttt gtatgcccaa agaaattcga
    20461 tttatgccaa gcgattttaa aaaaatgcaa gcttcttcgc ttatatcatc gggattgcat
    20521 tcgaagctgc attcagcatc gggtctaaga aatggtttga ttttgctaaa aaaagaagag
    20581 agggcttgct ttaaaacagg attttgcaag agataacttg gcgtcccacc accaaaataa
    20641 aggcttttta ttttttttat attaaattgt ttaatgcgaa aaaaaaactc ttcttctaaa
    20701 cggtcgatat accatgcggg aatatccctg caggcgagtg aataaaaatc gcagtagctg
    20761 cattttgaag cacaaaaagg tatgtgaata taaagtgcta cttccatagt ttataaaaaa
    20821 gaggcatcct taaaaagctg tactcgaaac aaagctttgc cttttatatt ttttgcatca
    20881 actggtcccc aaagacgaga atcaagattg gcaagtcgat tatcggagag aagaaagtat
    20941 tcgttctcag ctaaaacaat ggtttccata ttgcccaagg cgatatacaa gggatgactt
    21001 tcatcgacct ggccaagttc tttttgtatt tcatagtctt tttttgacat ttcaaattca
    21061 gttagaaaaa actgactatt cttatctttt acatagacaa taaaatcttt tatatacaga
    21121 ctatctccag gacacgcaag aacgcgaagg agcgaagagt tagaaaatgc ccgaggatgt
    21181 tcggtaaata attctatttt gttcaatgta aaaaaagaaa caatagcttt tgtgattttt
    21241 tttatctgac cgatatcctt tgttttcgac gtattcacca aaactaaatc gccatgactt
    21301 attttcatag atgaaggaat aagggtattt aacggtttta aaaaagggct aacaaaaacc
    21361 ctatccttat tttctgtaac agaaaaaaca gcatttgtct gaggaataaa agaaaaaagc
    21421 aaaaaggtct ttacaaaaaa aatagccgca atagtaaaaa caacaaaaaa aacaagagtc
    21481 gatattcttt tttgtagctg ccgtttcatt atataagaca attcgtaagg attttgtact
    21541 gacttattat ttatcatact aaaaatgtat cacaagaaag aagcaaatac aagaagcttt
    21601 ttagaaaagg attacttgac tttttttttc aaaattgcta cactcttttt tgttgagtgg
    21661 tacatctgta ccctacaacg cggatgtcgt ataatggcta ttaccctagc cttccaagct
    21721 ggtgacgtgg gttcgattcc catcatccgc taagcaaaag aaaactaccg aaaggtggtt
    21781 tttttttgca ttacaggttt aaagggaatc gatacgaagt ggtcggcatg caaacgtcct
    21841 gtttgcatgc ctta
//
LOCUS       DAOSSW010000022        21840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_2585_length_21840_cov_8.315079, whole genome shotgun sequence.
ACCESSION   DAOSSW010000022 DAOSSW010000000
VERSION     DAOSSW010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 21840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21840
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2585_length_21840_cov_8.315079"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1341)
                     /locus_tag="PKW26_03315"
     CDS             complement(130..1341)
                     /locus_tag="PKW26_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ92629.1"
                     /translation="MKKKNLLVTLIILISVILISSGVYLAVQFSKPAIRISYINLPQN
                     LTVAFTKIAENSKESKKEHIEILFFDTYPTKPKELKRLLKSDLILSKSGSIPEALEAY
                     LEEFNLDYTSTLPSTIRAMGQINKKQKALPLVLDHFELAWNKKLISPFANPYEAPKSI
                     EEILRIGYLAKENTTTPLYPFVCAGGDDESLINLIGVLIEAFFGEKAWIETTQTAKTI
                     DPKDLLSMPENKAFLATLNILKEWRQTGFLHPEWFHMTKADVENFMESNLALFVSMSF
                     SDHRKISQKAIERYETSYMPASIAMKESRSLTIPSYLIMQIKTKKTNQLANTILKEIY
                     THEGQNILTNATSLAPTTSQAETSDKQASDVRLWAAASNKALPSLASALYKNPEDISS
                     LAKMIRDYLEQ"
     gene            complement(1384..2520)
                     /locus_tag="PKW26_03320"
     CDS             complement(1384..2520)
                     /locus_tag="PKW26_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92630.1"
                     /translation="MKKAKSSQSKKNKTALKKQPIKTYIFFAIALVAIIIGFTLITQN
                     YKPFIILLPQEEKTFDSWSLLVRTIEKELGLKRIRLSGYKNFKDLENQIARAGTQKIL
                     WAEVPHSSDYPLEQLIEKKLLQTVDMSSNKIFFPASILAASQTATNSEKEHYTLPLCL
                     NPIVKIRKINPSSDIFPFDLVVSAKNPKDALDIYTFFKAELAKTNENPEAKTVFASIQ
                     EAANTKYIQKNAHTFTKQDSIQYFFAEESKDLLISTEDLLSFTTEQAHAVQVQNLGSH
                     ITSELHSLIFPKTKNAKTKRLIEKSQDLLANKELQFILANTRNWIPAHIATVARNAQT
                     DFIRQGIRQSSLCFIPSLEIPAEAEAKNEFLAQLEKAFRSKINE"
     gene            2672..>4158
                     /gene="glpK"
                     /locus_tag="PKW26_03325"
     CDS             2672..>4158
                     /gene="glpK"
                     /locus_tag="PKW26_03325"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOQ92631.1"
                     /translation="MAKYIISLDQGTTSSRAIAFNHEGLSVASVNKEFAQYYPQDGWV
                     EHDANEIFSSQLAVLKELIIKQDIGTEEIAALGITNQRETVVLWDRLTGEPVHRAIVW
                     QCRRTASLCEALKKKGYEQKIREKTGLLIDAYFSATKIQWILDAVAGLRERAERGEIL
                     AGTVDSWLIWKLSDGRLHATDVTNASRTMLFNIHTLEWDEDLLALFSIPSCILPEVRE
                     SSEVYCMTDEKVCGFSVPIASAIGDQQASLFGQACIAPGESKTTYGTGCFMLMHTGEK
                     AVLSKNNLLTTIALGYKGKITYALEGSVFMGGAIIKWLRDELGLISSAPECDVLAELV
                     PDSAGLVLVPAFTGLGTPYWDMYARGLLIGLTRGVNKAHICRAALEAIAFQVKDIIHC
                     MEEDASSVLSCMKVDGGASVSEILMQFQADMLDTKVLRPKNTETTALGAAYLAGLAVG
                     FWQDEEELFSTWQKDKSFEPKMQSEKREELYARWKKAVERSRSWL"
     assembly_gap    4159..4258
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4259..5704
                     /locus_tag="PKW26_03330"
     CDS             <4259..5704
                     /locus_tag="PKW26_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOQ92632.1"
                     /translation="AIIGAGIIGSTLARLLSRYELSICLIEKENDVSCGTSKANSGII
                     HAGYDAWPGTKMAEYNVRGNALYEGLSKELGFGFQRLGSYVVAQNDEELKSIEALFER
                     GKKNGVPGIEIISGFEFLKKEPHANPDILAALWAPTAAIISPYEATWAFAENAAENGV
                     DFFFEREVQGLEKVADIFHIKTGKGNIQARFVVNAAGLFSDRINAMVGARAFSIRPRR
                     GEYVLLDTNVKGLAKSILFQPPTSAGKGVLVTPTVDGNILIGPTAEPVQEKTATETTE
                     KGQRNILEVAKRTIPELTKRFAINSFSGLRATPFIDDKQYADFIIEEDGEVEKFFTLG
                     GIASPGLAAAPAIAEAVRNLLDTAGLVLKEKKDFKAHRKPFVYFARASEEEKQKLVRE
                     NPLYARIICRCEMVTEAEIIKAIHSPIGAMDLDGVKRRTRAQMGRCQGGFCLPRIAEI
                     ISRERAISMTEVSKSGRSSFVLQGKTREGIE"
     gene            5701..7062
                     /locus_tag="PKW26_03335"
     CDS             5701..7062
                     /locus_tag="PKW26_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ92633.1"
                     /translation="MKKAEKILKIKRWDIVIVGGGPAGLAAALQAHMRGIENILVIER
                     DIHLGGILQQCIHNGFGLHHFKEELTGPEYAQRFIDEIEGKIDYLLDAMVVDLDFQEH
                     DFQGVDDSENLREKIKKVSVMSPELGYQVFETRALILATGCRERTRGALLIPGTRPAG
                     IFTAGSVQRFVNIEGYLPGKKAVILGSGDIGLIMARRLTLEGIDVKLICEIMPYSSGL
                     TRNIVQCVENFNIPIKYNHTISQIHGRHRVEGVSVCEVDAERRIKPETEFFVECDTLL
                     LSVGLIPENEISLSSGILMDPMTSGPLINESMASSIEGVFACGNAVHVHDLVDFVSEE
                     GIKAGDAAADYIASMKDGKNGKKESEALRIPIRAGSRVRYVVPQFLDTKKKNIKLFFR
                     VTEEIKNCYINIESHTEQLRSLKKRIVKPGEMLQVELSPEQLLKVQGDLTVSIDLAQE
                     VMD"
     gene            7062..7439
                     /locus_tag="PKW26_03340"
     CDS             7062..7439
                     /locus_tag="PKW26_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOQ92634.1"
                     /translation="MTELIKENLVCIVCPMGCLLEVETRIENGRKKVLSVSHNECKRG
                     EVYAGKELVEPVRTLTTTVAVQGGSEVLLPVKTDGEIPKALLFQSMEILKRIRVKAPI
                     KMGDLVFADILGTGVDVVACKNI"
     gene            7482..13265
                     /locus_tag="PKW26_03345"
     CDS             7482..13265
                     /locus_tag="PKW26_03345"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOQ92635.1"
                     /translation="MKKTYLSFLLVAILVLIFSCTNTKASREESAEGIRFQNEGEFRV
                     LSFEPEGELPASVKNPQIYVQFSKPVVALKKLGEVMTSSDVFSIEPKLDGVYRWYGTS
                     LLAFEASDLTIPQMEYKVEVNPALVSIDGETIKGETSFRFKTQALRMLSVIPGYSAQK
                     EGVYVNNNDVPLDLAKDIALVFSYPVNPDVIKDYLEIYADDVKLNFSVLKAYENLLHV
                     SIEDTVPEDKKISVLLLEGGRSEKHFIGTEKRQAVSFQSLRPFVQTRMYTEGQSYSKY
                     TNPLEITYSHNLDTNQIDEIIKGIKTTPSMPLTKDNVEILGRTIRIFGLPVVYEQNYS
                     FSMDAEVKDIYGRSIVEKIRALVSVPPARSFVDFKDYGFALLEAEFAPKIAFEFQNIL
                     EGSEYRVNDFLQKFDAEMIPKNKRVTHLVDLEPHLKKEVGEKWGWAIFSANMRYKYKT
                     YDGEYKEREQTNTQEIQVTDIGASLRYAYNKAVVLVSSLSKAEPIANARVRFYSSPME
                     RSFSLLRQRKTLNFVAEGKTNSEGLAIINFAPGEYRRQFFGKNIFVEIETENPKDLVI
                     FNPNILNMYRYDVSSVRNPEDAEKEFMKTFIFTDRALYKPGETLTFRGIDRTLQRGEY
                     KPYVGNYEIKIQSGYWNPTVYATINGKTTATGGFWERFEIPPDLKPGSYDIVYRRTGL
                     NDLGTKGSCSFEIAFFERLRFEAKASISPGTYFRGDRLDGIVKAEYLGGGSLANANCY
                     VSWYSEPSGFYLNTKEYKDYRFGPLLGYEGRNFLNREEGLLMMDGSASFSQTSGSEKL
                     RGMAYQYRFEARVRDPGNQEIASVASAVVHPASFYLGIKKTGSSFGFVKKGDAISYDY
                     ILVDPNGNLPETALWNSKANEGKIKVELEREEWKAVHQIAVTGQLNTRYVKEMVSEST
                     SSIKIQKSGSFTIKPQNAGSYLLRVSTVDSKGREVVSELRFYATGASWASFYGNNSAQ
                     EIQLVADKSIYTVGDTAYIMLQSPLPQGQYLISVEREGIFSERLIRLDEASTVLEVPI
                     EDEYLPIVYVSVSSYSKRTGSTAEDYDTPDVNKPKGYFGLVRLEVDTITRAFDIAIST
                     DKESYRPGEKARISLKATKDGKPLSNAEITLMAVDRGVIDLINYHVPNPINFFYNRSN
                     FTYNVKGGDSRSYLIDPVTYAVKNLAGGDADEEDDDKLSERKNFDPTALFEPYLLTNA
                     AGEVSCEFVLPDSLTEYRITAIGIDKNNYALSEEKLPVNNPLSARHVLPQRLRVNDIS
                     EAGMVISNLDSVPHEVKLSLKTHEGIERSGEKAEKNGYLRGPGKASLKGEETKKITVK
                     PNETLSVFFEIEALKAGFVSLEFTLESAVLNERIINVLEIDKQYLYETVTTVGQVGSS
                     KKDEKSKENLTELIEIPGDAEDNMGKLYVQLDPTRLGLLRTAVQYVFDYPYGCLEQRS
                     ARILPLVYFGEYLKVFDLDSQVADPKKVVQDELAYWAKSQLFDGGFPYWPGGLNSTVD
                     VSIRIGEIVAQAKRHGIVVPASLNVKGLASFLKKQKTEMQKRWSAASSVYYYSYINYV
                     LSLMGETVEVKELEEITKSKSADIASLAFTGLTYFELDNKAKAKEVAAMLKKHMKMTT
                     QGVEISRIKTAPWWSFLNSNIADYALCLKLFTSLDPDDRLVPNLVHELLDMQNASHGY
                     WISTANTARALDAFDTYIKTNKLENLDFKASASLSGKELLSGSFKGLSSELIENDFYF
                     AGDALKKLPRDTELPLTFTKDGVGKLFYTSSLTYALPINQQSPRDEGIGLFVDYIDVE
                     TGKIVQETKLKSGKVYKARIFVSTTMDRNFVALRVPIPSGAEVVNAAFVTTGSYAEYD
                     EGREDESGYEGAYGNIAFSVGLSNRDIFDNEVQYFWNYFPKGRQTVEFMFRAVRKGMY
                     ETPSATAECMYESEIFGRTAGRLITIE"
     gene            13258..>15482
                     /locus_tag="PKW26_03350"
     CDS             13258..>15482
                     /locus_tag="PKW26_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOQ92636.1"
                     /translation="MNKIGRNKKSGGARKNALLDFCREFFKGLFKGLFNVSANALVKG
                     SVNVSAKGPTKGFFSQGKEKKKHQGKKRQTKRFLVFARILAFFFLFVCLCFFLAYLLP
                     WKALDTFSHRQYSTRFYDRRGDLLYILPLEEGLHREWLDLEAIPKHLVEAFIEAEDKN
                     FWHHHGVDFKAIFRAAGQNLKTKKRVSGASTISMQLARLIVPRKNPRASFGDKFFEML
                     RAIRLELKRTKTEILELYLNGIPFGFQCEGLQSAAKFFYGKEVYDLRADEIAELVLIP
                     RNPNLYAPTREKQYRYPNEAPHFLQYLISQESRFPPELFVSIDKNLNDIAQNLLLQSI
                     QDYDYARISNGAVFAIDNRTGEILVWLGNKDWYNSFDGQVDGVLTRNQAGSTMKAFLY
                     ALALERGFSPATVLPDVPMDFGGEEVYVPQNFNNRFNGPQLFRTSLASSLNIPAVYLL
                     WRLGIDDYVEKLLDLGFESLRDVRGSQGLSMALGSAEVSLFELVRAFSVFANEGELIK
                     PRAYMLENREEKNNKAKKSEVKKLEKKVAVFSSDTANIIADMLSDKEARSLGFGFPKV
                     FDTPYPAIFKTGTSNQFQNILALGATPAFTVGAWMGNLSGETVLGETGSSIPAKIVRS
                     LLDILEEERLTKGGEEMAFNEPLHFQKKYLCALSGMSPSPYCKNLVEEFCLSGDESSE
                     SCSWHYLNLASNSIAIRYPDEFQRWFASPNLQGSLFSSSALRFLYPLDRARFILDRGM
                     S"
     assembly_gap    15483..15582
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15673..16377)
                     /locus_tag="PKW26_03355"
     CDS             complement(15673..16377)
                     /locus_tag="PKW26_03355"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOQ92637.1"
                     /translation="MTKKKIASLLLSSVLMISLFAAGSSETKNTISVMGNGSVIVDSD
                     IAHIRVSVLTTDADASLSAQKNADLMTRVQDALIKIGLTKADFATSNYSIYENKPYNS
                     KDGSYGPPEYRTSNNLSVTVKDVSKTGLVIDTALKAGANQLSSLSFSSSKIAEAYDEA
                     RIQAIKDAQKKAEVLAREAGRKLGKAIIIEESHSYAEPIAYAGVMMLKAERADTPIVA
                     EDSTATYAVRVVFELK"
     gene            complement(16496..18643)
                     /locus_tag="PKW26_03360"
     CDS             complement(16496..18643)
                     /locus_tag="PKW26_03360"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOQ92638.1"
                     /translation="MILLEANDISYTYPHMKKKALDSVSLEIYSASYTAILGENGSGK
                     STLGKILAGILVADSGSIIAHEKPVGIVFQSPKHQIIAGEVLKDSSLGPENMGKSRKD
                     AKKIAREALKEIGLAEKMDANTLSLSLGQMQKLAMAGIIALSPKTIIFDEALSMIDPG
                     GRQEILALIDDLHERGKTIISITHDEDEAKKAEHIYFMQEGKIQSIIDKKEFENDADL
                     RAFFHYPLDTEQKQAIKKEDKKKPTISIDSVSFSYQNAKQKTEKGKDFFQNLSLQFCS
                     SSLTAIMGPSGSGKTTLFELIAGLLEAKDGEILSSGKVSLSLQDANAALFEEFAVDDI
                     AFGPRNQGLTGKKLLERVQKAMALVNLDYDVFKDKKTLALSGGQKRKLSLAGIIALDS
                     DILLFDEPTAGLDPKSRSEIMATMKALANAGKTVLFSTHRKEEGAFADRLILLEEGTV
                     IYDEAPSKIRQEEKEGLKTVKRSEGKILAGLQRGIGSDYMKKNTALHKAKPLPSFLLF
                     LGLFIFGIALQSPKSLGALSLFSILYAIISQFPLKKIIKRIFLVLPWIAFLLFFQILF
                     FGTRPGDIVYTSISFFSKWTLSLTSAKIELCIKTFLHFFNVLIALSVYNFTKTETEII
                     EGFEDFLKPLSYIKLPVKYIKLLFLLVFRFIPLLLDEAASILKTQIIREGIKETKGLF
                     KTVKIFLPLFIPLVLQTIRRAEYLSDALIARQY"
     gene            18690..19406
                     /locus_tag="PKW26_03365"
     CDS             18690..19406
                     /locus_tag="PKW26_03365"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HOQ92639.1"
                     /translation="MKQFFLVLMSIFSLFFFFSCQSTKRLELSQKRETENTNYYEIEI
                     EKPLLKDSVPYAKKINAHIDEIIGAQRASFLKIVRDYTEDWGGTHKLAFFVSWKLYQL
                     DDAIFSSLLTVYVYAGGAHGYTMLFPINYDLETGEEITIDTLPLYIGKKTKKKEKTSS
                     EFLETASRLSKPKIFSQVDTKHNTELAINWIEEGLEPKKENFSYFTLTEKNLIFHFAE
                     YQLAPYYYGRLEASIPYKKF"
     gene            complement(19445..20623)
                     /locus_tag="PKW26_03370"
     CDS             complement(19445..20623)
                     /locus_tag="PKW26_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ92640.1"
                     /translation="MNERKDKFSTKMLETPPSGIRKFFDLIIGRDDIISLGVGEPDFP
                     TPWLMREEAYYHLEQGHTSYTSNWGLLELREEIAKYLQKYNLDYNPKKEILVTIGVSE
                     AVDAVLRAILNPQDEIIICEPCYVSYQPLAELCDTKLIRFDTSNTNFYPTAEELEKVI
                     SPKTKAIMLCSPSNPTGKMIPKDELEKIAKLAQKHKIWVLSDEVYSELVYDENAHCSI
                     ASFPGMKDYAIVLNGFSKAFAMTGWRIGYVCCPPDLMEQVHKLHQYSTICAPIMSQYA
                     AAEGLRSGASEVEKMRLSYQQRRNLMTSKLDEMDIPYIEPEGAFYIFVDIRKTGLSAE
                     DFATKLINEYQVAVVPGSVFGKAGEGFVRCCYATEIKKLKIALERIKKMVQDLQRKNS
                     "
     gene            complement(20623..21108)
                     /locus_tag="PKW26_03375"
     CDS             complement(20623..21108)
                     /locus_tag="PKW26_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOQ92641.1"
                     /translation="MEEILQLLQDDGRISIEDMAAMTKKTSEEVKAIMKKLENDGTIV
                     KYAAIVNTDKLLNNENRVRAIIEVQVTPEREHGFDAIAERIYRFPEVKSLTLMSGGYD
                     LQIIIEGKSLQEVAFFVARKVSAIEGVKSTKTHFVLKTYKENDIVLVDQKRDRREGVT
                     A"
     gene            21285..21650
                     /locus_tag="PKW26_03380"
     CDS             21285..21650
                     /locus_tag="PKW26_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOQ92642.1"
                     /translation="MQFTGSIPIYEQIMRYIKKEIITGELNGEDKIPSVRDLADRLSV
                     NPNTVSRAYQELEREGLTETRRGMGTYIVGDKTMKDRIKKELSQDLVLDFVEEMKASG
                     FTAEEIIGFVSERLEKETL"
     gene            21650..>21840
                     /locus_tag="PKW26_03385"
     CDS             21650..>21840
                     /locus_tag="PKW26_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92643.1"
                     /translation="MKNLFKVCPSCGNKTISFEEGKKWTCAFCNFELYFNVATSASII
                     LLVGNALVLVRRGKEPGKGL"
BASE COUNT         6195 a         3873 c         4929 g         6643 t
ORIGIN      
        1 caccaaaacg agatagttcc ttttttcatg gacgaaaaaa ggaacgtata aatcctgttg
       61 actcgaacag tctatacatg gacgccgcag ggattggctt ggctcgtttt gcttatatcg
      121 atatgttttt tattgttcta gatagtctcg tatcattttt gcaaggctgc tgatatcttc
      181 agggttctta tacaaggcgc ttgcaaggga tgggagggcc ttatttgaag cagcagccca
      241 gaggcgtaca tcacttgctt gtttgtcgct tgtctcagct tggctggtag tgggtgcaag
      301 gcttgtagcg tttgtcaaaa tattttgccc ttcatgggta tatatttctt ttaaaattgt
      361 gtttgcgagt tggtttgttt ttttcgtttt tatttgcatt ataagatagc ttggaattgt
      421 tagtgagcga gattctttca tagcgatgct tgctggcata taagaagttt cgtatcgttc
      481 tatagccttc tgagatattt ttcggtggtc tgaaaaactc atgcttacaa aaagtgcaag
      541 gttactttcc ataaaatttt ctacatcggc tttagtcata tgaaaccatt caggatgtaa
      601 aaatcccgtt tgtcgccatt ctttaaggat atttaaggtg gctaagaaag ctttattttc
      661 tggcatagag agtagatctt ttgggtctat tgtttttgcg gtttgtgtcg tttcaatcca
      721 cgccttttct ccaaaaaatg cttctataag aacacctatt aggtttatga gggattcgtc
      781 atcgcctcct gcgcatacga aggggtatag tggtgttgtg gtattttctt tagctaggta
      841 tccaattcta agtatttctt cgatgctttt aggagcttca tagggatttg caaaaggtga
      901 tattagtttt ttattccatg ccaattcaaa atggtcgaga actaagggga gggctttttg
      961 ttttttattt atttgcccca tagcgcgaat tgttgaggga agggtgcttg tgtaatcgag
     1021 attaaattct tctaaatacg cttctaaagc ttcggggatt gaaccagatt tgctcagaat
     1081 aagatcgctc tttaagagcc ttttcaattc ttttggctta gttggatatg tgtcaaaaaa
     1141 caatatttct atatgttctt ttttactttc tttgctattt tcagcgattt ttgtaaaagc
     1201 cactgttaga ttttgaggca ggtttatata gcttatacgt atggctggct tggaaaattg
     1261 tacggcaaga tagactccgc tacttatgag gatgactgag atcaggataa ttagggtcac
     1321 caagaggttc ttttttttca tagtccaatc atagcataag agagaataat tcgctagctt
     1381 tttttattca tttatcttgc ttctaaaggc tttttctagt tgggctagga attcattttt
     1441 tgcctctgct tctgctggaa tttctaaaga gggaataaaa caaaggctac tttggcgaat
     1501 gccttgacgg ataaaatctg tttgagcatt tctcgctacg gttgcaatat gtgcgggaat
     1561 ccagtttcgt gtatttgcaa ggataaattg aagttctttg tttgcgagga ggtcttggga
     1621 tttttctatc aaccgtttgg tttttgcgtt tttagttttt gggaaaataa gtgaatgcag
     1681 ttctgacgta atgtgacttc cgagattttg tacttgaacc gcatgggctt gttctgtcgt
     1741 aaaactgagg aggtcctctg ttgaaataag caggtcttta gattcttctg caaaaaaata
     1801 ctgtatgcta tcttgtttgg taaacgtatg agcgtttttc tggatatatt ttgtatttgc
     1861 tgcttcttga atagaggcaa aaacagtttt tgcttcaggg ttttcgtttg tttttgccaa
     1921 ctctgcttta aaaaaagtgt aaatatcgag agcatctttg ggattttttg ctgaaacgac
     1981 gaggtcaaat ggaaaaatgt cgcttgaggg atttattttt ctaattttta cgatgggatt
     2041 caggcaaagg ggaagcgtat aatgttcttt ttcgctattt gttgctgttt ggctggcggc
     2101 taaaatcgat gcgggaaaaa agattttgtt gctgctcata tcgacagttt gcaatagttt
     2161 tttttctata agctgttcta ggggatagtc tgaagaatgg ggaacttcag cccaaagaat
     2221 tttttgtgtg cctgcccgtg ctatttggtt ttctaggtct ttaaagtttt tatagccaga
     2281 aagacgaatt cgtttgagcc caagctcttt ttctattgtg cgaacaagaa ggctccatga
     2341 atcaaaggtt ttttcttctt gaggtagcaa tataataaaa ggtttatagt tttgggtaat
     2401 gagtgtaaaa cctatgatga tggcgacaag cgctattgca aaaaaaatat aggttttaat
     2461 gggctgcttt tttagagctg tcttattttt tttcgattgg gaagattttg cttttttcat
     2521 agcctaagta taatagaaga aagggctaaa agcaattaaa aaaaacacct attgtatctg
     2581 atttttttta agataacata aggaaaggca ttttttatct aggttttagt ttttttcttg
     2641 catttcctct tatcgatgct atacttttag tatggctaaa tatattattt ctctcgatca
     2701 ggggacaacg tcttctcgtg caattgcctt caatcatgag gggctttctg ttgcttcggt
     2761 aaataaagaa tttgcgcagt attatccgca agatggctgg gttgaacatg atgcaaatga
     2821 aattttttct tcacaactgg ctgtcttaaa agagctaatt atcaagcagg atattggtac
     2881 ggaggaaatt gctgcgcttg gtatcacaaa tcagcgggag acggtggttt tgtgggatag
     2941 gcttacgggt gagccagtgc atcgggcgat tgtctggcag tgtcgacgaa cggcttctct
     3001 ttgcgaggct ttgaaaaaaa agggttatga gcaaaagatt cgtgaaaaaa cggggctttt
     3061 aatcgatgcg tatttttctg caacaaagat tcaatggatt ctcgatgcgg tagcgggttt
     3121 gcgtgagcgt gcggagaggg gagaaattct tgcagggact gttgacagct ggcttatatg
     3181 gaagctttct gatggaaggc ttcatgcgac tgatgtgaca aatgcgagtc gaactatgct
     3241 gttcaatatt catacattgg aatgggatga ggacttattg gctttatttt ctattccaag
     3301 ctgtattttg cctgaggttc gggaatcgag tgaggtctat tgcatgactg atgaaaaggt
     3361 ctgtgggttt tcggttccta ttgcttcagc tattggggat cagcaagctt ctttgtttgg
     3421 gcaggcctgt atagcacctg gagaaagtaa aacgacgtat ggaacgggtt gttttatgct
     3481 catgcatacg ggtgaaaaag cggttctttc aaaaaataat ttacttacaa cgatagctct
     3541 tggttacaag ggaaaaataa catatgccct tgagggtagt gtttttatgg gtggcgctat
     3601 tatcaagtgg cttcgtgatg aactcggtct tatttcctca gctccagaat gtgatgtttt
     3661 agcggagttg gttcccgatt ccgcaggcct tgttttggtt cctgctttca cgggtcttgg
     3721 gactccctac tgggacatgt atgcgcgcgg tttgcttatt gggctgacgc gtggagtaaa
     3781 taaggcgcat atttgtcgcg ctgccttaga agcaattgct tttcaagtaa aggatattat
     3841 tcattgcatg gaagaagatg cctcatcagt gctctcttgc atgaaggttg atggcggtgc
     3901 atcggttagt gaaattctca tgcagtttca agctgatatg ctcgatacaa aggtgctgcg
     3961 tccaaagaat acagaaacaa cagccctcgg ggcggcttat cttgcaggtc ttgcggtggg
     4021 tttttggcag gatgaagagg agcttttttc tacttggcaa aaggataaaa gttttgagcc
     4081 aaagatgcag agtgaaaaaa gagaagagct ctatgctcga tggaaaaaag cggttgagcg
     4141 aagtcgctcc tggcttgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
     4261 gataataggg gcaggaataa ttggctcgac tctggcgcgt ttgttgtcgc ggtatgagct
     4321 ttctatttgt ctcatagaaa aagaaaatga tgtttcttgt ggcacttcaa aggcaaactc
     4381 tggcattatc catgctggct atgatgcctg gccgggaaca aagatggccg aatacaatgt
     4441 gcgtggaaat gccttgtatg aggggctttc aaaagagctg ggttttggct ttcaaagact
     4501 cggtagttat gttgttgcgc aaaatgacga agagttaaaa agcattgagg cacttttcga
     4561 gcgtggcaaa aaaaacggag ttccagggat tgagataatt tctggctttg agtttttgaa
     4621 aaaagagcct catgcaaatc cagatattct cgctgctttg tgggcgccaa ctgcggcgat
     4681 aatttcaccc tatgaagcga catgggcttt tgcggaaaat gctgcagaaa atggagtgga
     4741 ttttttcttt gagcgagagg tgcaaggtct tgaaaaagtg gcggatattt ttcatatcaa
     4801 aacgggtaag ggaaatattc aagctcgctt tgtcgtaaat gccgcagggc tttttagcga
     4861 caggataaat gcaatggttg gggcgagggc tttttctatt cgccctcgtc gtggtgaata
     4921 tgttttactc gacacaaatg taaaaggtct tgctaagagt attttgtttc agcctccgac
     4981 atctgctgga aagggagttc ttgttacgcc cactgtcgat ggcaatattt taattggacc
     5041 aactgctgag ccagttcaag aaaaaacagc gacagagaca acggaaaaag ggcagagaaa
     5101 tattttggag gtagcgaagc gcaccattcc agagctgaca aaacgttttg cgataaattc
     5161 tttttcgggt ttgcgcgcaa ctccttttat agatgataaa caatacgctg attttatcat
     5221 agaagaggat ggcgaggtag aaaagttttt tacgctcggt ggcatcgctt cgcctggttt
     5281 agctgcagcg ccagctatag ctgaggcggt tcgcaactta ctcgatacgg cagggcttgt
     5341 gctaaaagaa aaaaaagatt ttaaagcaca tcgcaaaccc tttgtgtatt ttgcacgagc
     5401 aagcgaagaa gaaaagcaaa aactggtgag agaaaatcct ttgtatgcac gtattatttg
     5461 tcgctgtgaa atggtaactg aggctgaaat catcaaggcg attcactcgc ctattggagc
     5521 tatggatttg gatggagtaa aaaggcgaac gagggcgcaa atggggcgct gtcagggagg
     5581 tttttgtttg ccacggatag cggaaattat tagccgagaa agagctatct cgatgaccga
     5641 ggtgagtaaa agcggccgca gttcttttgt cttgcaggga aaaacacgag agggaataga
     5701 atgaaaaaag cagaaaaaat tcttaaaata aagcgatggg atattgtgat tgtaggcgga
     5761 ggacctgcag gtctcgccgc tgccttgcaa gcgcatatgc gtggtataga aaatattctt
     5821 gttatcgagc gagatataca tcttggtgga attttgcagc aatgtattca caacggtttt
     5881 ggcttacatc attttaaaga ggagcttact ggaccagaat atgctcagag gtttattgac
     5941 gagatagagg ggaagataga ctatctcttg gatgctatgg ttgtcgacct tgattttcaa
     6001 gagcacgatt tccaaggagt tgacgactca gagaacctga gggaaaaaat aaaaaaagtt
     6061 tcggtgatga gtcctgaatt gggctatcaa gtgtttgaaa ctcgcgctct tattcttgca
     6121 actggttgta gagagagaac tcgaggtgcc ttgcttattc caggaacgcg tcctgctggc
     6181 atttttactg cgggatcggt tcagcgcttt gtaaatatcg aaggctatct tcccggaaag
     6241 aaggcggtga ttcttggctc tggcgatatc gggcttatta tggctcggcg tttgactctt
     6301 gaaggaatcg acgtaaaact tatttgtgaa attatgccgt attcatcggg gcttacgcga
     6361 aacattgtgc aatgtgtaga aaattttaat atacctataa aatataatca tacaatttct
     6421 caaattcatg gaaggcatcg ggtggaagga gtgagtgttt gtgaggttga tgccgagcgt
     6481 agaataaaac cagagacaga attttttgtc gaatgcgata cgcttttgct ttctgtgggg
     6541 cttataccag aaaacgaaat ttcactttca tcaggtattt taatggaccc gatgacgagt
     6601 ggaccgctta taaatgaatc gatggcgagc tcaatcgagg gagtttttgc ttgtggaaat
     6661 gctgttcacg tgcatgacct tgttgacttt gtgagcgagg aaggcataaa ggcaggggac
     6721 gctgctgctg attatattgc atccatgaaa gatggaaaaa atgggaaaaa agaaagcgaa
     6781 gccttgcgca ttcctattcg agcagggagt cgtgttcgct atgttgtgcc gcagtttttg
     6841 gatacaaaaa agaaaaacat aaaattgttt tttcgcgtaa cggaagaaat aaaaaactgc
     6901 tatatcaata ttgaatcgca tacggaacaa ttgcgctctt taaaaaaacg cattgtaaaa
     6961 cctggcgaga tgttgcaagt ggaattaagt cccgagcaac ttttgaaggt gcagggagac
     7021 ttgactgttt ctatagacct tgcgcaggag gtgatggact aatgacagag ctaataaaag
     7081 aaaatcttgt ttgcattgtt tgtcctatgg gttgtctttt ggaagtggaa acaaggatag
     7141 aaaatggaag aaaaaaagtg ctttctgttt cccataacga atgcaagagg ggcgaggttt
     7201 acgcaggaaa agaacttgtt gaaccagtta ggacactcac aacgactgtt gctgtgcaag
     7261 gtgggtctga agttttactt cctgtaaaaa cagatggaga aataccaaag gcacttttgt
     7321 ttcagagcat ggaaatatta aaacgcatta gagtgaaggc gccaataaaa atgggagatc
     7381 ttgtttttgc tgatattcta ggaacggggg tagacgttgt cgcctgtaaa aatatataaa
     7441 tatattctat aaataaaaac gatataaaaa ggatttgagt tatgaaaaaa acgtatttat
     7501 ctttcttgct tgtagctatc ttagtcttga ttttttcttg tacaaataca aaagcaagtc
     7561 gagaagaaag tgcagaggga attcgctttc aaaatgaagg tgagtttcgg gttctaagtt
     7621 ttgagccaga aggggaactg cctgcttctg taaaaaatcc gcaaatctac gtacagtttt
     7681 caaaacctgt tgttgcattg aaaaaactcg gcgaagtgat gacaagctcg gatgtgtttt
     7741 ctattgagcc aaaacttgat ggagtgtatc gctggtatgg aacaagcctt cttgcttttg
     7801 aagcaagcga tttaaccata ccgcagatgg aatacaaggt agaagtaaat cctgcccttg
     7861 tttctataga cggtgaaacg ataaaaggag aaacgagttt tcgttttaaa acacaggcgc
     7921 ttcgtatgct ttcagttatt cctggctata gcgcacaaaa agaaggcgtc tatgtaaaca
     7981 acaacgatgt tccccttgat ttagcaaaag atattgccct tgtgttttct tatcctgtaa
     8041 atcccgatgt gataaaagac tatcttgaaa tctatgctga tgatgtaaag ctaaactttt
     8101 ctgttctcaa ggcgtatgaa aatcttttac atgtttcgat agaagatact gttccagaag
     8161 ataaaaaaat ttctgttctc ttgcttgaag gtggacgatc tgaaaagcat tttattggta
     8221 cagaaaaaag acaggcggta agttttcaaa gcttgcgtcc ctttgtacaa acaaggatgt
     8281 atactgaagg gcaaagttat agtaagtata caaacccctt ggaaataaca tatagccata
     8341 acttggatac aaaccaaatc gatgagatta taaagggaat aaaaacgact ccttcaatgc
     8401 ctcttacaaa ggacaatgta gaaatcttag gaaggacgat tcgtattttt ggcttacccg
     8461 tagtgtatga gcaaaattat tcttttagca tggatgcaga agtaaaagat atctacggaa
     8521 gaagtatagt tgaaaaaatt cgtgcccttg tatcagttcc tcctgctcgc agctttgtag
     8581 actttaaaga ttatggcttt gcccttcttg aagcggagtt tgctcccaag attgcatttg
     8641 aatttcaaaa tattttagaa ggctcggaat atcgagtcaa tgattttcta caaaaatttg
     8701 atgcagagat gattccaaaa aacaaacgag ttacccatct tgtcgactta gaacctcacc
     8761 taaaaaaaga agttggcgaa aaatggggtt gggctatctt ctccgcaaat atgcgttata
     8821 agtacaaaac ctatgatggc gaatataaag aaagagagca aactaatacg caagaaattc
     8881 aagtaacaga tattggggca agtttacgct atgcctataa caaggctgtc gttctcgtct
     8941 cgagtttaag caaggctgaa cccatagcaa atgcgcgggt gcgcttttat tcttctccga
     9001 tggaacgctc cttctctctt cttcggcagc gaaaaacttt aaactttgtt gcagaaggaa
     9061 aaacaaatag cgaaggctta gctatcatca attttgcacc gggagaatat cgcaggcagt
     9121 tttttgggaa aaacatcttt gtagaaatag aaacagaaaa tcccaaagac cttgttatct
     9181 ttaatccaaa tatcttaaac atgtatcgct atgatgttag ttcggtgcgt aatcccgaag
     9241 atgcagaaaa agaatttatg aaaaccttta tctttaccga ccgcgccttg tataagccag
     9301 gagaaacact taccttccgc ggaatcgata gaaccttgca acgtggcgaa tataaaccct
     9361 atgtgggaaa ctatgaaatt aaaattcaaa gtgggtattg gaacccgacg gtctatgcaa
     9421 ctatcaatgg aaaaacaacc gcgacgggtg ggttttggga gcgctttgag ataccgcctg
     9481 acttaaaacc tggttcatac gacattgtct accgaagaac gggattgaat gacttgggaa
     9541 caaagggctc ttgctccttt gagatagctt tttttgaacg gcttcgtttt gaagcaaagg
     9601 cttctatttc accggggact tatttccgtg gcgataggct tgacggcatt gtaaaggctg
     9661 agtatctcgg aggcggaagt ttggcaaatg caaactgcta tgtcagttgg tatagcgagc
     9721 cgagcggttt ttatctcaac acaaaggagt acaaggacta tcgttttggt cccttgcttg
     9781 gttatgaggg gcgaaatttt ctaaaccggg aagaggggct tttgatgatg gacggctcgg
     9841 cgagctttag ccaaacgtct ggaagcgaaa agcttcgcgg tatggcctat caatatcgct
     9901 ttgaagcacg agtgcgagat ccaggaaatc aagaaattgc ctctgttgct tcggctgttg
     9961 tgcatccggc ttctttttat ctgggcataa aaaaaacagg ctctagtttt ggctttgtaa
    10021 aaaaaggtga tgctatcagt tatgattata tcttagttga tccaaatgga aatcttcccg
    10081 aaactgctct gtggaactcg aaggcaaatg agggaaaaat aaaagttgag cttgaaagag
    10141 aagaatggaa ggcggtgcat caaatagctg ttacggggca attgaacacg cgctatgtaa
    10201 aagaaatggt aagcgagtca acttcttcta taaaaataca aaagtcaggt agctttacga
    10261 ttaaaccaca aaatgcaggc tcctatctct tgcgtgtttc aacggtcgac agcaagggtc
    10321 gagaggtggt aagtgaattg cgtttttatg caacaggcgc ttcctgggct tctttttatg
    10381 gaaacaatag tgcacaggaa atacaacttg tcgctgacaa atctatctac acagtaggcg
    10441 ataccgctta tattatgcta caaagtccac ttcctcaggg acagtatttg ataagcgtcg
    10501 aacgggaagg aattttttct gagcgtctca tacgtcttga tgaagcaagc acggttttgg
    10561 aagttcctat agaagatgag tatctaccca tagtctatgt ttctgtctcc tcgtattcga
    10621 agcggacagg ttctactgca gaagattatg atactcccga tgtaaataaa cccaagggct
    10681 actttggttt agttcgcctc gaagttgaca ctatcactcg cgcctttgat atcgctatta
    10741 gtaccgataa ggaaagttat aggccgggag aaaaggctcg gatttctctc aaagcaacaa
    10801 aggatggtaa gcctctttca aatgcagaaa ttaccttgat ggctgttgac cgtggtgtta
    10861 ttgatttaat aaattatcat gttccaaatc caattaactt tttttataat cgctcgaatt
    10921 ttacttataa cgtaaaggga ggcgattcac gttcgtattt aattgacccg gtaacctatg
    10981 cagtaaaaaa tctagcaggg ggtgatgctg acgaagaaga tgacgacaaa ctgagcgaga
    11041 gaaaaaattt tgaccctacg gccttgtttg agccctatct tttgacaaat gcagcgggtg
    11101 aagtttcttg tgagtttgtc ttgcccgatt ctttaaccga gtatcgcatt accgctatcg
    11161 gtatcgataa aaataattat gccctaagcg aagaaaagct tccggtaaat aatccacttt
    11221 ctgctcgcca tgttttaccg caaagacttc gcgtaaatga tatttcagaa gcaggtatgg
    11281 ttatatcgaa tcttgattct gttcctcatg aagtgaaact ctctcttaaa acgcatgagg
    11341 ggattgagcg ttcaggtgag aaggcagaaa aaaatggcta cctgcgaggt cctggaaaag
    11401 caagcttgaa gggagaggaa acaaaaaaaa tcacggtgaa gccaaacgaa acgctttctg
    11461 ttttctttga gatagaagcg ttaaaggcag gctttgtcag tttagaattt acgcttgaat
    11521 cagctgtttt aaacgaacgc attatcaatg tactcgaaat cgacaagcag tatttgtatg
    11581 agacagttac aacggtgggg caagttggct cttcaaaaaa agatgaaaag tcaaaagaaa
    11641 acctaacaga gcttatcgaa ataccagggg atgccgaaga caatatgggc aagctctatg
    11701 tacagcttga tccaactcgc cttggtttac ttcgcacagc tgtgcaatat gtttttgatt
    11761 atccctatgg ttgtttggag caacgtagtg cgcgtatttt accgctcgtg tattttggtg
    11821 aatatctcaa ggtctttgac ttggactccc aggtagctga tccaaaaaaa gttgtgcaag
    11881 atgaacttgc ctactgggca aagagccagc tttttgacgg aggctttccg tactggccag
    11941 gtggtttgaa ttctacggtg gatgtgagta tacgcattgg agaaattgtt gcacaagcaa
    12001 aacgccatgg cattgtggtg cctgcaagtt taaatgtaaa aggtcttgcc agtttcttaa
    12061 aaaagcaaaa gacggaaatg caaaaacgct ggtctgcggc aagttcggtt tattattata
    12121 gctatatcaa ttatgtctta agcttgatgg gagaaacagt agaagtaaaa gagttggaag
    12181 aaataacaaa gtcaaaatct gctgatatcg cgtctcttgc ttttacgggg cttacgtatt
    12241 ttgaactgga caataaagca aaggcaaaag aagtcgctgc aatgcttaaa aagcatatga
    12301 agatgacaac gcaaggagtt gaaatttctc gaattaaaac agcaccttgg tggagttttt
    12361 taaattcaaa tattgccgac tatgctttgt gcttaaaatt gtttacgagt ttggatccag
    12421 atgacaggct tgttccaaat cttgttcatg aacttctcga tatgcaaaat gcgagccatg
    12481 gctactggat atccaccgca aataccgctc gcgctcttga cgcctttgat acatacataa
    12541 aaacaaataa gttggaaaat ctcgatttca aagcgagtgc aagtctttct ggaaaggaat
    12601 tgctctcagg ctcattcaag ggcttgtcga gcgagcttat tgaaaatgat ttttactttg
    12661 caggagatgc attgaaaaaa cttccacgcg atacggaact tccattaact tttacaaaag
    12721 acggtgtggg aaaacttttt tacacaagct ctctaactta tgcgcttcct atcaatcagc
    12781 aaagcccgcg agatgaaggc atcggtcttt ttgtcgatta tatcgatgta gaaacaggaa
    12841 agattgtgca agagacaaag ttgaaatcag ggaaggtgta taaggcgcgg atttttgttt
    12901 ccacaacaat ggatagaaac tttgttgcct tacgtgttcc cattccttct ggcgccgaag
    12961 tggtaaatgc tgcctttgtt acgacaggaa gctatgccga atacgacgaa ggaagagaag
    13021 atgaatcggg ctatgaagga gcatatggga acatagcttt ttctgtaggc ctttcaaacc
    13081 gtgatatttt tgataacgaa gtgcagtatt tttggaacta ctttccaaag ggcaggcaga
    13141 cagttgaatt tatgtttaga gctgttcgta agggaatgta tgaaacgcca tcggcaacag
    13201 ctgagtgcat gtatgaaagc gaaatctttg gacgaactgc aggacggcta ataacaattg
    13261 aataagatag gtagaaataa aaagtcggga ggggcgagaa aaaacgctct tctcgatttt
    13321 tgtagggaat tttttaaggg cttgttcaag ggcttgttta atgtctcggc taatgccttg
    13381 gttaaaggct cggttaatgt ctcggctaag ggtccaacaa aaggattttt ttcacaaggc
    13441 aaagagaaaa aaaagcatca ggggaaaaaa cgccaaacaa aaagattttt agtttttgct
    13501 cgcattcttg cattcttttt cctttttgtt tgcctttgtt tttttctcgc ctaccttctt
    13561 ccatggaagg ccctcgatac tttttcgcat cgccaatact caacgcgctt ttatgacaga
    13621 cggggagact tgctctacat tcttcccctt gaagaaggct tgcatcgaga atggctggac
    13681 ctcgaagcta tccccaagca tcttgtcgaa gcctttatag aagcagaaga taaaaacttc
    13741 tggcatcatc acggagtcga tttcaaagca atattccgcg ccgctggaca aaatctcaaa
    13801 acaaaaaaaa gagtcagcgg cgcctctacg attagcatgc agcttgcccg cctcatcgtg
    13861 ccaaggaaaa atcctcgggc aagctttggc gataagtttt tcgagatgct tcgggctatt
    13921 cgcttggaat tgaagcgaac gaaaacagag atactagaac tctatctaaa cgggataccc
    13981 tttggatttc aatgcgaggg tttgcaatct gctgcaaagt ttttttatgg aaaagaagtc
    14041 tatgatttgc gtgcagatga aatagctgaa ttggttttaa ttcctcgaaa cccaaacctc
    14101 tacgcaccga ctcgggagaa acagtatcgc tatccgaacg aagcgccaca ttttttgcag
    14161 tatcttataa gtcaggaatc tcgcttcccg ccagagcttt ttgtatcgat tgataaaaat
    14221 ctcaatgaca tcgcacaaaa tcttcttttg caatcgatac aagactatga ttacgcacgg
    14281 atttcaaacg gcgctgtttt tgcaattgat aatcgcacgg gagaaatact tgtttggctt
    14341 ggaaataaag attggtacaa tagttttgac gggcaggtcg acggggtttt aacgcgcaat
    14401 caagcaggta gtacgatgaa ggcattttta tatgcccttg ctcttgaacg cggcttttct
    14461 cctgcgacgg ttcttcccga tgttcccatg gattttggag gcgaagaggt ttatgttcca
    14521 caaaatttta ataatcgctt taatggtccg caacttttta gaacctcttt ggcttcaagt
    14581 ttaaatatac ctgcggtcta tcttttatgg cgactgggca ttgacgatta tgtagaaaaa
    14641 cttttagacc taggctttga atctcttcgt gatgttcgag gtagccaagg tttgtcgatg
    14701 gcgcttggca gtgcggaagt aagcttgttt gaacttgtgc gggctttctc ggtttttgca
    14761 aatgaaggag agcttataaa accgagggcc tatatgctgg aaaatcgcga agagaaaaac
    14821 aataaagcaa aaaaaagtga agtaaaaaag cttgaaaaaa aagtcgctgt tttttcatct
    14881 gataccgcaa atataattgc agatatgcta tcggataagg aagcgcgctc tctcggtttt
    14941 ggtttcccaa aagtttttga cactccctat cctgcaattt ttaaaacagg aacttcaaat
    15001 cagtttcaaa atatcttagc cttgggggca acacctgcat ttaccgttgg agcctggatg
    15061 ggaaacctaa gcggtgaaac agttctaggc gaaacaggaa gctctattcc tgcaaaaata
    15121 gtgcgaagcc ttttagatat actcgaagaa gagcggctta caaagggcgg tgaggaaatg
    15181 gcttttaatg aaccgcttca ctttcaaaaa aaatatcttt gtgcgctttc tggaatgagt
    15241 ccctctcctt actgcaaaaa tctcgtagaa gaattttgct taagcggcga tgaatcgagt
    15301 gagtcttgtt cctggcatta tctaaacctt gcaagcaata gcatagcgat tcgctatcct
    15361 gatgagtttc aaagatggtt tgcctctccc aatttacaag gcagcttgtt tagctccagc
    15421 gctttgcgtt ttttatatcc cttggacagg gcacgattta ttttggatag aggtatgagc
    15481 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaatttgg aaaacgtttt
    15601 atagacattc gataaaggcg agacacactt ttatcgaatg ctaagtctaa actaataaaa
    15661 tacttgttac gattatttta attcaaaaac aactcgtact gcatatgtcg cggtactatc
    15721 ttcagcaaca atgggtgtat cggctcgctc tgcttttagc atcataactc ccgcataggc
    15781 gataggttct gcatagctat ggctctcttc aatgataatt gccttgccga gtttgcgacc
    15841 agcctccctt gcgaggacct ctgctttttt ttgtgcatct ttgatagctt gtatgcgtgc
    15901 ttcatcgtag gcttctgcaa ttttactcga tgaaaaactc aaggaagaaa gttgatttgc
    15961 acctgccttt aaagcggtat cgattacgag ccctgttttg gaaacatctt ttactgttac
    16021 actcagatta tttgaagtgc gatactcggg agggccatag ctcccatctt ttgaattgta
    16081 tggcttgttt tcgtagatgg aataattcga cgttgcaaaa tcagcctttg ttaaacctat
    16141 tttgatgagt gcatcttgta cccttgtcat aaggtctgca tttttttgtg cagaaagaga
    16201 agcatctgca tccgttgtta aaactgaaac gcgaatatgt gctatgtcgg aatcgacgat
    16261 aacagaacca tttcccataa cggagatggt gttttttgtt tcgcttgaac ctgctgcaaa
    16321 aaggctaatc attaaaacac tacttaaaag taggcttgca atttttttct ttgtcatttg
    16381 tgctcctaaa aaattttgct ctgtaaacag gctgtcttat atttccctgt tattattaat
    16441 ataacaattc aaacggaaaa aggaaacaaa agcgaaaaga ttgcgacgat tttttttaat
    16501 actgccgtgc gataagcgca tcgcttaaat attctgctcg ccgtattgtt tgtaaaacaa
    16561 gggggataaa taagggtaga aatattttta ccgttttaaa gagccccttt gtttctttta
    16621 tgccttcacg gataatctgt gtttttagaa tcgatgcggc ttcatcgagg agcaagggga
    16681 taaaacgaaa aaccaataaa aataaaagct tgatatattt taccggtagt tttatgtagc
    16741 tgaggggttt caaaaaatct tcaaagcctt ctatgatttc tgtttcagtt tttgtaaaat
    16801 tatatacgga gagtgctatg agtacgttaa aaaaatgcaa aaaggttttt atacaaagtt
    16861 cgatttttgc acttgtaaga gaaagcgtcc attttgaaaa aaaggaaatg cttgtgtaga
    16921 ctatgtcgcc aggtcttgtg ccaaaaaaaa gtatttgaaa aaacaagagg aaggctatcc
    16981 agggaagtac caagaaaata cgctttatta ttttttttag ggggaactgt gaaataattg
    17041 catagaggat cgaaaaaaga gaaagagcgc cgagactttt cgggctttgt aaggcaatgc
    17101 caaaaataaa gagaccgaga aaaagtaaaa aagaggggag cggttttgcc ttgtgaagag
    17161 cggtgttttt tttcatataa tcgctgccaa tgcctctttg taagccagcg agtatttttc
    17221 cctcagagcg ttttactgtt ttgagtcctt ctttttcttc ttgccttatt ttagaaggcg
    17281 cttcatcgta aatgactgtg ccttcttcta gcaagatgag tctgtctgca aaggctcctt
    17341 cctcttttcg atgtgtagaa aagaggacgg ttttacccgc attggcaagt gctttcattg
    17401 ttgccataat ttcagagcga cttttggggt caagcccggc tgttggctca tcaaaaagaa
    17461 gaatgtcgct atcaagggcg atgatgccgg cgagagaaag ctttcttttt tgtccgccag
    17521 atagggctaa ggtttttttg tctttaaata cgtcgtagtc taagttgaca agagccattg
    17581 ccttttgcac tcgctccaag agttttttcc cggtaagccc ttgattgcgt ggcccaaagg
    17641 caatatcatc tacagcaaat tcttcgaaca aggctgcatt tgcatcttgc aaggaaaggg
    17701 aaaccttccc cgacgagaga atttctccat cctttgcttc gagtaaacca gcgatgagtt
    17761 caaaaagcgt tgtttttcct gagccagagg gtcccattat tgcagtcaat gaggatgagc
    17821 aaaattgcag tgaaagattt tgaaaaaaat ctttgccttt ttcagttttt tgttttgcat
    17881 tttgatagga aaatgataca gaatcaattg agatagtagg ctttttttta tcctcttttt
    17941 ttattgcctg tttttgttct gtgtctaggg gataatggaa aaaagctcgt aagtcggcat
    18001 cgttttcaaa ttctttttta tcgattattg actgtatttt tccctcttgc ataaaataaa
    18061 tgtgttctgc tttttttgcc tcatcttcgt cgtgcgttat gcttataatt gtttttcccc
    18121 tttcgtgcaa gtcgtcgatg agtgcgagta tttcttgccg tccaccggga tcaatcatgg
    18181 aaagtgcttc gtcaaaaata attgttttcg gagagagtgc gataattcct gccattgcta
    18241 gtttttgcat ttgccctagg gagagtgaga gggtgtttgc atccattttt tctgcaagac
    18301 ctatttcttt gagtgcttct cgggcgattt tttttgcgtc ttttctactt tttcccatat
    18361 tttctggacc gagggagctg tcttttaaaa cttctcctgc aattatttga tgtttcggtg
    18421 attgaaagac tattcctact ggtttttcat gggcgataat acttccagaa tctgcgacca
    18481 agatacccgc taaaatcttt ccgagtgtgc tttttcccga gccgttttcc ccgagaatgg
    18541 ctgtatagga agctgagtag atttctagcg aaacagaatc gagcgccttt tttttcatat
    18601 gcggataggt ataggaaata tcgtttgctt ccaaaagaat cataaaaaat ataatacctg
    18661 ttttatacaa aatatgctat aataaaaaca tgaagcagtt ttttttagtc ctcatgagta
    18721 ttttttctct cttttttttc tttagttgtc aaagtacaaa gcgtcttgag ctttcacaaa
    18781 aacgcgaaac agaaaataca aattattatg aaatagagat tgaaaaacct ctgttgaaag
    18841 actcagttcc gtatgcaaaa aaaataaatg cccatatcga tgagattatc ggtgcacaaa
    18901 gagcgagctt tcttaagatt gttcgggact atacagaaga ctggggtggc actcacaagc
    18961 ttgccttttt tgtttcttgg aagctctatc aactcgatga tgctatattt agctctttgc
    19021 taaccgtcta tgtctatgcg ggtggcgccc atggctacac aatgcttttt cccattaact
    19081 atgatttaga aacaggggaa gaaattacca tcgatacgct ccctctatat attgggaaaa
    19141 agacaaagaa aaaggaaaaa acgtcctcgg aatttttaga aacagcttcc agactaagca
    19201 agccaaagat tttttctcaa gtagatacaa agcataatac agaactggct atcaactgga
    19261 ttgaagaggg cttagagccg aaaaaagaaa atttttccta ctttacactg actgaaaaaa
    19321 atctgatttt tcattttgca gagtatcagc tcgcacccta ctactatggg cgcttagagg
    19381 cttctattcc ctataaaaaa ttctaaccga ggaaagtata gaagaaagca aactcttgta
    19441 ttgcctagct attttttcgc tgtaggtctt gaaccatttt ttttattcgt tcgagcgcaa
    19501 tctttagttt ttttatttca gtggcgtaac agcaacgaac aaagccttct ccagccttgc
    19561 caaaaacaga acccggcaca accgcaacct gatattcatt tataagtttt gttgcaaaat
    19621 cttcagcgct caagcccgtc tttcgtatgt cgacaaaaat gtagaaggcg ccttctggtt
    19681 cgatgtaggg aatatccatt tcatcgagct tgcttgtcat aaggtttcgt ctttgttggt
    19741 aggaaagccg cattttttca acttcgcttg caccgcttcg caaaccttct gccgctgcat
    19801 attggctcat aatcggtgca caaatcgtcg aatactggtg tagtttgtgg acttgctcca
    19861 tcaaatcggg cgggcagcaa acatagccga ttctccaacc ggtcatggca aaggcttttg
    19921 aaaaaccgtt taggacaatc gcgtagtcct tcatgcccgg aaaggaagca atagaacaat
    19981 gcgcattttc gtcgtagacg agttcggaat agacttcgtc agaaagcacc caaattttat
    20041 gcttttgtgc gagcttggct attttttcaa gttcatcttt gggaatcatt tttcccgttg
    20101 gattgcttgg cgagcaaagc ataatagcct ttgttttggg acttatcacc ttttcaagtt
    20161 cttcagcagt tggataaaag tttgtatttg aggtgtcaaa gcgaataagc tttgtgtcgc
    20221 agagttctgc aagcggttga taggaaacat agcagggttc acaaataata atttcatcct
    20281 gcggatttag gattgcacga agcaccgcat cgacagcttc agaaactcct atggtaacaa
    20341 gtatttcttt ttttggatta taatcaagat tatacttttg tagatatttt gcaatttctt
    20401 ctcgtagctc gagcaatccc cagtttgatg tgtaggaagt gtgcccctgt tcgagatgat
    20461 aataggcttc ttcgcgcata agccaaggag tgggaaagtc tggctcacca acaccgagtg
    20521 agataatatc atcgcgacct ataatgaggt caaaaaattt cctaatgcca gagggcggtg
    20581 tttccagcat ttttgtggag aatttgtctt ttcgttcatt cattatgcgg taaccccttc
    20641 gcgtcggtct cgtttttgat caactaaaac aatatcgttt tctttatagg ttttgagtac
    20701 aaaatgtgtt tttgtgcttt ttaccccttc aatcgctgaa acttttcggg caacaaaaaa
    20761 agcaacttct tgcaaggatt ttccttctat aataatttgc aagtcatagc caccagacat
    20821 gagtgtaagg gactttactt ctgggaatcg ataaatgcgc tcagctattg cgtcaaaacc
    20881 atgctctctt tcgggtgtaa cctgcacttc aataatagcg cgcactctgt tttcattgtt
    20941 aaggagtttg tcagtgttta caatggccgc gtattttaca attgttccat cgttttcaag
    21001 ctttttcata attgctttaa cttcttcgct tgtttttttg gtcatggcag ccatgtcctc
    21061 gattgaaatg cgtccgtcat cttgtaataa ttgtaaaatt tcttccatta ttgtctcctt
    21121 tttttctgcg cgatgcagca gaagtgaaaa tatacagaca gtatagttat ctttgcataa
    21181 atatccaata cttataccct tattttggaa attatttaaa aaaagacttg acattgttga
    21241 attagtgtgc taagttaata atacactaat tcaaggaggt gcttgtgcag tttactgggt
    21301 ctataccgat ttatgaacaa ataatgcggt atataaaaaa agaaattatt acaggcgaac
    21361 tgaatggcga agataagatt ccatcagttc gagaccttgc agacagactt tcggttaatc
    21421 caaataccgt ttcaagagcc tatcaagagc tggagcgtga aggtttaact gaaacacgcc
    21481 gtggaatggg gacctacatt gtaggagata aaacaatgaa ggatagaata aaaaaagagc
    21541 tcagccaaga tctggtgctc gattttgttg aagagatgaa ggcttcaggt tttaccgcgg
    21601 aggaaatcat aggctttgtt tcagaacgtt tagagaagga gactctttaa tgaaaaattt
    21661 atttaaagta tgcccgtcgt gtgggaataa aacaatttcg tttgaagaag gcaaaaaatg
    21721 gacctgcgct ttttgcaatt ttgaacttta ttttaatgtt gcgacgtcag catctattat
    21781 tcttcttgta gggaacgccc ttgttttagt acgccgaggg aaggagccag gaaaggggct
//
LOCUS       DAOSSW010000023        21272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_2668_length_21272_cov_7.800207, whole genome shotgun sequence.
ACCESSION   DAOSSW010000023 DAOSSW010000000
VERSION     DAOSSW010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 21272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21272
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2668_length_21272_cov_7.800207"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PKW26_03390"
     CDS             <1..417
                     /locus_tag="PKW26_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOQ92644.1"
                     /translation="YGYVYVARIAMGANMAQTIRALREAESYDGPSIVIAYSHCINHG
                     INMGNGLQNQKDAVTSGLWPLYRYDPRLVEQGKNPFQLDSKEPDFNLADYMYKEVRFK
                     TLKAANPERAQMLLEKATEKAKRQYKEYKYLAEREF"
     gene            528..1238
                     /locus_tag="PKW26_03395"
     CDS             528..1238
                     /locus_tag="PKW26_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOQ92645.1"
                     /translation="MFRTLIYFFKLVWDLLKKNKDLKKAEKLEKQGRIKERDALVRPI
                     VQDWAQKLIYNTKSTVEVIGSENVPKNQSIVLIGNHQSYMDIPLLLGFIDTPLAFISK
                     AEILKVPIIARAMKQMQCEFMERNNPRQSLQTILKAIENIKNGYSTVIFPEGTRSKGG
                     PVIDFKPGSFKLAFKSGAPIVPISIDGTWRLYEDKKRINAGHIRLTIHNPIETSKLTK
                     EEMHELPEKIRDIILAAL"
     gene            1247..2113
                     /locus_tag="PKW26_03400"
     CDS             1247..2113
                     /locus_tag="PKW26_03400"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOQ92646.1"
                     /translation="MLKKEILIKKASGHLEPFDPRKLLQSLNVSGADERLAKEILSEI
                     EAWLKPEVSTKKIYAKALGLLRQKTVSAAGKYRVKEALMELGDNGHPFEIFVGEIFKK
                     LNYEVEVGKILQGKSITHEMDVIATKEKNQHLVECKYSQFDSRYVSIQVPLYVHSRVQ
                     DIIAHRKKEDAFRGFKFTGWVATNNRFSPDSVKYAEDYGLSLLSWNYPKEYSLRYLIE
                     KHNLFPVTILQSLNTKEKEKLIANNIVTCNALFNNLEHLVALDIPEKRKKILLAELNA
                     INNKNTHKNNAK"
     gene            2245..2625
                     /locus_tag="PKW26_03405"
     CDS             2245..2625
                     /locus_tag="PKW26_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92647.1"
                     /translation="MKKLLLFVLIISLSLFFLSAQSTETSESLKFIESSVKAGNATID
                     FSFNDLTGVLRLTYTLETFTFDLDEADRLIREAVATFVKENGYFKYRVYPEEDDIRYF
                     AKTRTTRYKKFYILYDKARLEGSY"
     gene            complement(2701..3255)
                     /locus_tag="PKW26_03410"
     CDS             complement(2701..3255)
                     /locus_tag="PKW26_03410"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOQ92648.1"
                     /translation="MRKTYVGLSILLFLVGLVMIFSPEGWIKACVIIIGFSILLNGFY
                     NIIKMRVLLVNPFFQKAILVRGILSILVGIFAVLLPLLVAKLVFSVLLYGIAVYLCIA
                     SVIQGYALYKIKDAGFSIAPYLYEIVFSIVLALVFIFIPTEVGKSIVRIFGIIVSLAA
                     IGFIIYEWKSKPYIIQAESVEDID"
     gene            3368..3949
                     /locus_tag="PKW26_03415"
     CDS             3368..3949
                     /locus_tag="PKW26_03415"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOQ92649.1"
                     /translation="MKNKSLSKKASLTALFSALICATAFIQIPLPIGVPIVIQDMTAI
                     LAGMLLGPLYGTLSVLLFLVVGIIGLPVFSGKAGFHVISQGLTGGFLVGYLLSAFLAG
                     LCMQFFYKPEKNEKSAKTAFKSWLVLFFAAVIATVALFTLGIARFMTIGEKSFLQSIS
                     LVLLPFLPGNLIKLGLVVILTKRLHRFVQEITQ"
     gene            3974..4729
                     /locus_tag="PKW26_03420"
     CDS             3974..4729
                     /locus_tag="PKW26_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ92650.1"
                     /translation="MDKIKEAKNKTLFVLQNISKTFYPKFQALTNITFSVNKGDFIVI
                     AGANGSGKTVLMHIIAGLEEASKGELIFFKEQRVGLVFQEADSQILAETVAEDVAFGP
                     KNAGLSKEAVKQKVHESLQWTSLLEKKDFSSRLLSGGEKRRLALAGILALDSDIFIFD
                     EPFANLDWPSVRQVCELLEKLKKEGKTILVLTHELEKILAYANRFIILSQGRAVFDGS
                     VEEGLSEQMLTKLENWGIRNPLQSYKKKEDLHW"
     gene            4723..5463
                     /locus_tag="PKW26_03425"
     CDS             4723..5463
                     /locus_tag="PKW26_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HOQ92651.1"
                     /translation="MVVNLFSYRKAESLLHKANPGLKLVFLILFSSLIFLKQTWIAPL
                     LLTALSFCLALLLFFMAKIPVKKIFQLKLLVFIGFLFALFKSLSLDIQFSPRFQFLCS
                     FSLQDGLAGLFWGFNFFTASFFALIFFDTTSMSDLQDSFHSVQKKLSRFIPILEKIPL
                     ANALALTISFIPEVFSLWGQIKKASLARQNTRSHVLTKPKGVFKKKYKKKLSLRRQLA
                     VFLQEFSTLISLLLLRAMEKRKALLNRS"
     gene            5518..6183
                     /locus_tag="PKW26_03430"
     CDS             5518..6183
                     /locus_tag="PKW26_03430"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOQ92652.1"
                     /translation="MKALIVGNGSLENIDLLKKEAKKADFIICADGGAGFLEKASLLP
                     HFLIGDFDSIDQGLLDFYRSKNVEIIQHPTKKDFTDTELAIELALEKKARAISLIAVT
                     GSRIDHSLSSLFLLKPLIEKKIDAKILSDCNEVYLLGPGESSIKFEKKNLSLNMDELI
                     LSLLALSEKVEGIRTTGLSYPLVDACLKQGSSLGVSNRFSAPSATIRIKKGFLYIILS
                     KEI"
     gene            6203..7249
                     /locus_tag="PKW26_03435"
     CDS             6203..7249
                     /locus_tag="PKW26_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002687824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ92653.1"
                     /translation="MKYSFKNDYAEGAHPQILQALAETNMLQSDGYGLDSFSEHAKSI
                     LYKHINFPQAQVHFLVGGTQTNLIALSSFLRPHEAVIAVSSGHILVHETGAIEATGHK
                     IISTKGKEGKIYIPELEALLKEHSDEHMVKPRLVYISNASEIGTIYSKEELETLYRFC
                     KDQNLLLYMDGARLGAALTAHGNNLSLSDIAASVDAFYIGGTKNGALFGEALVISNPS
                     LQKDFRFLIKQKGGLLAKGRLLAIQFLTLFQDNLFFDLAKHGNTMAGKIQKELKDLGF
                     SFFIESPTNQIFPIFPKTLIEELEKDYGFYVWEDISASEAAVRLVTSWATKEDAVKSF
                     IKAVEKSSTYNLKK"
     gene            7269..7862
                     /locus_tag="PKW26_03440"
     CDS             7269..7862
                     /locus_tag="PKW26_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOQ92654.1"
                     /translation="MKKKNVFIVYCHPSEESFTRNVRDSFIQGLEAAGHSYELSDLYA
                     MDFQSDMSKEEYTRDAFYHDIPVIGEDVKEEQEKINRADIIVFIYPVFWTDVPAKMKG
                     WFDRVLSYNFAYGNKTMKMLDKVLILCTAGNKIWHLKRFGLLRSMKKIMLGDRFFNRA
                     KKKDFVVFDGMAMSFPEHREKHWEIFKKKAYEIGKNL"
     gene            complement(7965..8546)
                     /locus_tag="PKW26_03445"
     CDS             complement(7965..8546)
                     /locus_tag="PKW26_03445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOQ92655.1"
                     /translation="MNTLILYCHPWEGSYNFAILKAITKGLEKRKVSYEILDLVLDGF
                     NPVMTEADLAEYRKGAFVDPKVGEYQEKLMRAEEIIIITPIWWGMPPAILKGFFDKVF
                     LPHWAYETAASGLLQGKLTHIKKATVISTMNSPQFYYNLFLKSPLKNSMILGTLKSCG
                     VQNIKWISFPNVLKQKQEIREKWLNKLERYAET"
     gene            8575..9486
                     /locus_tag="PKW26_03450"
     CDS             8575..9486
                     /locus_tag="PKW26_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ92656.1"
                     /translation="MIESILIEGLIYGIMVLGVFITFRILDFADLTVDGSFPIGASIM
                     GIFLLKGVNPFLALLVAFLGGLICGLITALIHTKLKIPGLLAGILTMTMVYSISLRIM
                     SNRANISYLKVPTLFSKIQAMSAAFLSPDLSILFFSILVVAVILVLLNNFFTTDFGLC
                     MGALGSNPQMIISQGMNPEIIKAVGIALSNGLVAFSGALASMYQGFADVGSGSGIAIA
                     GLASLMIGEFIIRSNKISALTFRVILGSILYRALMYLARKYGYLIHMNPNDLKLITGL
                     LIILSMVISKTKFRKKPSVHKAEASHA"
     gene            9479..10282
                     /locus_tag="PKW26_03455"
     CDS             9479..10282
                     /locus_tag="PKW26_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ92657.1"
                     /translation="MLKLENISKIFAEGSPDENRALKNINLEIHEEDFVTVIGSNGAG
                     KSTLYNVIAGTMKATSGIVSLKTKNGIKNITEDKEHIRAHYIGRIFQNPLLGTAGKMS
                     LEDNMVICSKKGYKGLKKSLNNKTRDYFRSQLEILGMGLENRLKDNVELFSGGQRQAL
                     TLLMTLMSKPSLLLLDEHTAALDPHMAQLVMDLTKKFASDDKLTVMMITHNMNHALSY
                     GNRLLMMDGGEIVLDIGAEEKAKLSMAEIISQFKAIRKKEIVNDQMLLQ"
     gene            10482..11414
                     /locus_tag="PKW26_03460"
     CDS             10482..11414
                     /locus_tag="PKW26_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOQ92658.1"
                     /translation="MQYITVKQASELWGISDRRVRLLCAEGRIEGLIQKGRSYLIPKN
                     TLKPLDGRSLRGKNIPEQYARLFTTIDEMKAELNRRRPLSIGELKRLQDEFLVEFTYN
                     SNAIEGNTLTLQETALALEGVTIDRKPLKDHLEAVGHRDAFLYVASLVSNKVAISERI
                     IREIHSLVLINKPEDKGIYRRIPVKIMGAHHEPPQPYLVPVQMEKLLTGLSRVDQHII
                     ETAALFHLDFEGIHPFVDGNGRTGRLIINLMLMQAGYPPIDMKFTDRRKYYSCFDSYY
                     RDKDASPMVKMIGEYVKERLSHYLSLVEDFHRPM"
     gene            complement(11579..11986)
                     /locus_tag="PKW26_03465"
     CDS             complement(11579..11986)
                     /locus_tag="PKW26_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOQ92659.1"
                     /translation="MKKILIDLNIILDFLNKRNYHKEAAILMDKCVKKEVEGFVCAHE
                     ITTLSYFLFKEKRDRSKIMQTLASLLDLCKVIALDKNILKEALISPIQDFEDAVIEVS
                     AIKEQVDYILSRNISDFKLSRIPAYTPEQFFLL"
     gene            complement(11967..12206)
                     /locus_tag="PKW26_03470"
     CDS             complement(11967..12206)
                     /locus_tag="PKW26_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HOQ92660.1"
                     /translation="MKKLTLSLDKDLIHFAHTYSRHNDISVSKLFEQYLLQLQSKGEK
                     QELDSKTQLLYGAFERDPIPNKEELRKGFYEKDTH"
     gene            complement(12290..12823)
                     /gene="msrA"
                     /locus_tag="PKW26_03475"
     CDS             complement(12290..12823)
                     /gene="msrA"
                     /locus_tag="PKW26_03475"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOQ92661.1"
                     /translation="MTKENSKQTIYFAAGCFWGVEEYFRRVHGVIESSSGYMGGHTEN
                     PNYIAVVDGETGHAETVRVVYDSNKLEFKELLARFFSIHDPTSLNRQGNDIGTQYRSA
                     VFYTKEGQKKEIDAMIEHLTKIQKYYDPILTEVLEAKVFYPAEEYHQRYIERRPFAYC
                     HVNLKDAIEPVDERFFA"
     gene            complement(12804..13658)
                     /locus_tag="PKW26_03480"
     CDS             complement(12804..13658)
                     /locus_tag="PKW26_03480"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ92662.1"
                     /translation="MNELIGNLVTKKKDTFFDTVIETETHSIPVRVYIPKELNEDAPL
                     VLYMHGGGFVLGTYAQKHLVTKQISQKAHCIVVSINYALAPEYPYPEGLMDCLAVYNY
                     FIANSSLENCQYRDVIVAGDSAGASLSAVICQYQRDKGEKIPLAQILFSPATAKINPK
                     TGDAWESRIKNQKKSFLTERSLEVFYDFYLEGKLEENLYNPYVFPLMTKDFSGLPPAA
                     FVLCGKDPLYDEGLAYAQALQKEGRVEIREFKNKDHNYQGKEAIDFVVAFIKSIERDW
                     GKDDKRKF"
     gene            13838..14011
                     /locus_tag="PKW26_03485"
     CDS             13838..14011
                     /locus_tag="PKW26_03485"
                     /inference="COORDINATES: protein motif:HMM:NF040920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1871A family CXXC motif-containing protein"
                     /protein_id="HOQ92663.1"
                     /translation="MGKLKKQMHQRPRYKDFVFALLFGLGVFLLIIGLKDGGFLSVFR
                     KAAFICLECIGIG"
     gene            14020..14874
                     /locus_tag="PKW26_03490"
     CDS             14020..14874
                     /locus_tag="PKW26_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOQ92664.1"
                     /translation="MKKYNFFTRIYIQIAAVFASNPFLQNFSKGTIYQGKGKGICVPG
                     LNCYSCPAAVGACPLGSLQSSLASPQGRTSFYIAGFLVSIGAFFGRTICSFLCPFGFL
                     QELLYKIPTKKIKKSKTTRRLSIAKYIFLVVFVLALPYGVYLATGIGSPFFCKLICPA
                     GSLEAGIPLVIANESLQSLIGFLFSWKMSLLALFIITSIFIYRPFCRFVCPLGAIYSF
                     FNKYALFGVRVDTAKCISCKKCINFCKLDVHEVNDRECIRCGECISVCPTEAISFFQK
                     RTNNNTIS"
     gene            14885..15478
                     /locus_tag="PKW26_03495"
     CDS             14885..15478
                     /locus_tag="PKW26_03495"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOQ92665.1"
                     /translation="MTKKILTLSFAFLILISFVFTSCAKNEEKEFAKAAEQLKKYQMV
                     EKKESEPTTQEVKTEAKKEGPMALDFSLQMTDGTKFTLLQNLDKPILVNYWATWCPPC
                     VREFPDLEEMYKNYKDRMHFVAINSAETKDVIEAFMTKNKYTIPVAMDSDNAVGMMYG
                     IASIPTTFIVDTEGRIVFAQLGMMTKKQMQNAIEAAL"
     gene            complement(15482..17578)
                     /locus_tag="PKW26_03500"
     CDS             complement(15482..17578)
                     /locus_tag="PKW26_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92666.1"
                     /translation="MNLKNHSQAEKVLLWRESLTKLSDQQFFDLLRMYIGEIKTPYNK
                     QNLIEQMSSVLRKEENKQKILSLLDYQDRLILAALHVFSGASFTQLKHFFSDSISSYD
                     LETDLQNLEERLLLFRSLEPSTEKKIYLINPLLEEALLPFIGSSVLFPKSKLHADIDS
                     ENTFFLNSHLLAAVYAFVFQFPDLCKIDGSLKKKHAQTISELFLNHCDISCFEKLISA
                     LQNMGLFKQVENETHIDHERWNEFSLLSEQEQYAYIAAASIASLPRSVLANLASICTV
                     FLMSLEENLYTRKILKRIAFVFFEKLHATTMPKVSKLRTLLQQNQAKKTELAFDSALL
                     VDSLISFGLLEALGIDDEDEALYKVRKTETSSDTKKLVHLGSSFFVSILPGLSLHSLL
                     PLVKIMDLQGCDTSLSFEITKQSVTRAFESGMKPDFILKTFEAYSVHEIPQNFHHAIQ
                     DWYANFKSGSLYKGFVLHVDADKIIAVENNAVLKKYLCHTLAPGVYLMNFANEDEAHD
                     AVQKSGLDFIGRIKTLAKPSISLPLSPLQHALIQSPIDFADTKTDEEKNHILLSKKRD
                     VQKELLDYLAEMDLTKEQREDLSLRIKKKIVFSTEQIRPDSVRYVKTEAKGMDFLGKI
                     AIVEQAISSELRLEIRYDGLEKEEKKVFVGKPLLIEKFTNDALVHLEVEEKKEPVQLS
                     VGGASLVKLIRKSFVF"
     gene            complement(17559..19316)
                     /locus_tag="PKW26_03505"
     CDS             complement(17559..19316)
                     /locus_tag="PKW26_03505"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOQ92667.1"
                     /translation="MNSQNPLIVQSDRTLLLDVHMPRSAECRDALLPFAELEKSPEHL
                     HTYRLTPLSLWNARSSGFSPEDVARVLMEFSRYEVPTSVLTWVTEVTSRFGKLILLPD
                     PLSPEDEERLILKVESQTIYREIEASPSLRKTLTPLLNANEYTFDSSYAFSLKLVDRG
                     SIKQDLMKLGWPVKDEVPLRDGEPLEVLLREKTLFGHAFEPRQYQVEAAQALVGNKGP
                     GSGFGTIVLPCGSGKTIVGMKIMSMLHTNTLILTTNISAVHQWIDELIDKTELTAEQI
                     GEYTGEKKTIKPVTVATYQVLTWRPDKNSAFPHFQIFKERPWGLIIYDEVHLLPAPVF
                     RVTAELQAIRRVGLTATLVREDGCEGHVFSLVGPKRYDVPWKELEGAGWIASAECIEL
                     RIDLASEKEIEYATVQPRLKHRLASENPSKIPLVMDLVRHFKNDKILIIGQYLQQLHE
                     IAVLLNAPIITGKTPTSQRDALYADFRSGKNSVLVVSKVANFAIDLPDASMAIQISGT
                     FGSRQEEAQRLGRILRPKERTSRFFTIITRNTVEEEFGANRQKFLAEQGYAYVLLRTP
                     EDIAQYLAENGESDESKKS"
     gene            complement(19321..20025)
                     /locus_tag="PKW26_03510"
     CDS             complement(19321..20025)
                     /locus_tag="PKW26_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92668.1"
                     /translation="MKNNILLIGKDFLENEAFVAYCKGKEIPFVCTTKAQAKSEGKET
                     VFEGTKSIIPWNRASNLSSMNLIIRAETLLDSIDRVLLYFDYDHFTEEYSQLGKNHFQ
                     IIADELIASYQYITENILERFKEKKQGGFIFLLKEALPLFRSSKNSVEVNNPALYAAQ
                     EAFVAFAEMTAYRYSQKLENSVVLTRTTLKDDEKIASLLFSLLESDKLEKKLQKDATR
                     WLLLQDQNEGAIRFLK"
     gene            20110..>21272
                     /locus_tag="PKW26_03515"
     CDS             20110..>21272
                     /locus_tag="PKW26_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOQ92669.1"
                     /translation="MKIWIKYVLASILGSLAAFLLPLNITSVQKTIDFLTEISIRFGR
                     YMLVPLLFFSMALAIYKLRNTGKLFKTAVYVATVSLITSFVLVFLGILTTLLIKLPRI
                     PISVEKMTNSASLDIPTRLLDIFPITSFSVFLDGNFLLPLLVLAGFIGAGCASDTNAS
                     KLTVNLFDSLSKVFFAVMSFFVDMFAFGMIFITCTWTILFKDLLRSGVFTPLILLLAG
                     EFILIAFILYPLVLKFVFKEKTPYRVLYASTASLITAFFSGDINLSLGVAIKHLKESL
                     GLKRRIAAVTAPVFSVFARGGTALVTATAFIVILRSYSGLVIPFGEIVWIGFISFLIS
                     FFLGGIPIGGAFLSLTILCSFYGRGFDAAYLLLKPMAFVLGSFATAIDTSTMM"
BASE COUNT         6675 a         4478 c         3833 g         6286 t
ORIGIN      
        1 tatggctacg tatacgttgc acgcatcgca atgggtgcta atatggcaca aaccatccgc
       61 gccttacgag aagctgaatc ctacgacgga ccatctatcg taatagcata cagccactgt
      121 attaaccatg gaattaacat gggcaatggc ttacaaaacc aaaaagatgc tgtaacaagc
      181 ggactttggc cattgtaccg atacgatcca cgacttgttg aacaagggaa aaaccccttc
      241 caactcgaca gcaaagaacc agatttcaat cttgctgact acatgtacaa agaagtacgc
      301 tttaaaacct taaaagctgc aaatcctgaa cgagcacaaa tgctcttgga aaaagcaaca
      361 gaaaaggcaa agagacagta caaagagtac aaatacttag ctgaaagaga attctaagca
      421 aagtaaaaca aaaggcggtt tgcataaagc aagtcgcctt ttttattgcc tagagctctt
      481 ttttggtgta gaatagtact atataaacca ttctctttgg gggcacaatg tttagaacac
      541 ttatatattt tttcaagctt gtttgggact tactgaaaaa aaataaagac ttaaaaaaag
      601 cagaaaaact tgaaaaacaa ggacgcataa aagaaaggga tgccctcgtg cgacccattg
      661 tacaagactg ggcacaaaaa cttatctaca acacaaaatc cacagtggaa gtaatcggtt
      721 cagaaaatgt acccaaaaac caatccattg ttttaattgg aaaccaccaa agctatatgg
      781 atattcccct tcttttagga ttcatcgaca ctcctcttgc ctttatatcc aaggctgaaa
      841 tactcaaggt tcccatcatt gcacgggcga tgaaacaaat gcaatgcgaa ttcatggaac
      901 gaaataaccc gcgacaatcc ttgcaaacca tcctcaaagc aatcgaaaac ataaaaaatg
      961 gatattcgac ggttatcttc ccggaaggaa cacgcagcaa gggcggtccg gtaattgatt
     1021 ttaagcctgg aagttttaag cttgcattta agtctggagc tcccattgtt cctatcagca
     1081 tcgatggtac ctggcgcctt tacgaagaca aaaaacgcat aaacgcagga catatacgcc
     1141 tcacaataca caatcccatc gaaacatcga agcttacaaa agaagaaatg catgaacttc
     1201 ctgaaaaaat tcgagatatt atcttagcag ccttatagga tagactatgt taaaaaaaga
     1261 aatactaata aaaaaagcat ctggtcatct tgaacccttt gacccacgaa aactcttaca
     1321 gtcgctcaat gtctcaggtg ccgatgaacg actcgcaaaa gaaattcttt ctgaaattga
     1381 agcttggtta aaaccagagg tgagcactaa aaaaatttat gcaaaggcac tcggccttct
     1441 tcgtcaaaaa acagtttctg ctgcaggaaa ataccgcgta aaagaagcct tgatggagct
     1501 cggcgacaac gggcatccct ttgaaatctt tgttggcgaa atatttaaaa aactcaacta
     1561 cgaagttgaa gtcggaaaaa tactccaggg gaaaagcatc acgcacgaaa tggacgtcat
     1621 tgcaacaaaa gaaaaaaatc aacacctcgt cgagtgtaaa tatagccaat ttgatagtcg
     1681 ctatgtaagc atacaagtcc cactctatgt ccattcacgc gtacaagaca ttatcgcaca
     1741 tagaaaaaaa gaagatgcct ttcgcggctt taagtttaca ggatgggttg caacaaacaa
     1801 tcgtttttcc ccagattccg taaaatatgc agaagattat ggactctccc ttttatcatg
     1861 gaattaccca aaagaatata gcttgcgcta tctcatcgaa aaacacaatc tttttcccgt
     1921 aacgattcta cagtccctca acacaaaaga aaaagaaaaa ctcatcgcaa acaatattgt
     1981 tacttgtaac gctttgttca ataacctcga acatctcgtt gccctcgaca ttccagaaaa
     2041 aagaaaaaaa atccttttag cagaattaaa cgcaattaat aataaaaaca ctcataaaaa
     2101 taacgcgaaa taaaaacttt gattgcaaaa tttcttcttt caatatatac tatactaagt
     2161 agtcgtgcaa atgctaaaag ctagtgagat acctcgcaat ccatagtgag gaaaaaatat
     2221 attgacttgt tataggagga ctctatgaaa aaattgctat tatttgttct cattatttcg
     2281 ctatctttgt tttttctcag tgcacaaagc acagaaacga gtgaaagttt aaaatttatt
     2341 gaatcaagtg ttaaagctgg aaatgctaca attgacttct cattcaatga tttgaccggt
     2401 gtactcagac ttacctacac gctcgaaacc tttaccttcg atcttgatga agcagataga
     2461 cttatccgcg aagcagtagc aacatttgtc aaagaaaacg gatattttaa gtatcgcgtg
     2521 tatccagaag aagacgatat ccgctatttt gcaaaaacac gaacaacacg atacaaaaaa
     2581 ttctacatct tatatgataa ggcacgttta gaaggctctt attaacaaat aaagcaccac
     2641 tgtagaaaaa caatcttcta actagtggtg cattctttga tttacttaat taaaccccta
     2701 tcaatcaata tcctcgacag attctgcttg aataatatag ggctttgact tccattcgta
     2761 gataataaaa cctatggcag caagagaaac aataatacca aaaatccgta ctattgattt
     2821 tcccacttcc gttggtataa aaataaacac aagtgccaaa acaatcgaaa aaacaatttc
     2881 ataaagatag ggcgcaatgc taaaacctgc atcttttatt ttatagaggg catagccttg
     2941 tattacactc gcaatacata aataaacagc aatcccatac aacaaaacag aaaaaacaag
     3001 cttggcaacc aacaagggta acaatacagc aaaaattcct acgagaatgc tgagtatacc
     3061 tctaactaaa atcgcttttt gaaaaaatgg gttcacgagt aggacccgca tttttataat
     3121 attgtaaaaa ccattgagca aaatactaaa tccaataatt atgacacagg cttttatcca
     3181 gccttctggt gaaaaaatca taaccaagcc aactaaaaac aataaaatag acagtccaac
     3241 ataggttttt ctcatccatc tcctcctaca tgtgctttta ccttttataa gtatacgcta
     3301 gattggacaa aatgaaaaca aaaaaatccc cttgcataca tttttattat ttgttaccct
     3361 caaaaagatg aaaaacaaat cccttagtaa aaaagcaagc ctaaccgctc ttttttctgc
     3421 ccttatctgt gcaacagcct ttattcaaat ccctctccct ataggcgttc ccatcgttat
     3481 ccaagacatg accgcaattt tggcaggaat gcttctagga cctctgtatg ggactctttc
     3541 tgttttactc ttcttagttg tgggcatcat cggacttcct gttttttcag gaaaagccgg
     3601 atttcacgtt atttcgcaag gtcttactgg tggtttttta gtaggctacc ttctctcggc
     3661 ttttttggca gggctttgta tgcagttttt ttataaacca gaaaaaaatg aaaaatcagc
     3721 gaaaacagca ttcaaatcat ggcttgttct tttttttgcc gcagtcatcg caacagtcgc
     3781 tctctttact cttggcatag cacgatttat gaccataggc gaaaaatctt ttctacagag
     3841 tataagcttg gtcctccttc cttttttacc tggaaacctt ataaaacttg gacttgtcgt
     3901 cattctcaca aaacgcttgc atcgatttgt tcaagaaatt acacagtaag tgtcaaataa
     3961 aacaggatgc aagatggata aaataaaaga agcaaaaaat aaaacacttt ttgtgttgca
     4021 aaatatttcc aaaacctttt atccaaaatt ccaagcactt acaaatatta ccttttctgt
     4081 aaacaaaggc gactttatcg ttattgctgg ggcaaatggc tctggaaaaa cagttctcat
     4141 gcacattatt gcagggcttg aagaagcaag taaaggcgaa cttatttttt ttaaagaaca
     4201 acgagttggc ttggtatttc aagaagcgga ttcacaaatc ttggcagaaa ctgttgcaga
     4261 agacgtcgcc tttgggccaa aaaacgctgg tctttccaaa gaagcggtta aacaaaaagt
     4321 ccacgaaagt cttcagtgga catccttact tgaaaaaaaa gatttttctt caagactctt
     4381 atctggtgga gaaaaaaggc gactcgccct agcaggaatt cttgccctcg acagcgatat
     4441 ttttattttc gatgagcctt ttgcaaacct tgactggcca agcgtcaggc aagtctgtga
     4501 acttctcgaa aaacttaaaa aagaaggaaa gacaatcctt gtcttaacgc acgaacttga
     4561 aaaaatactc gcctatgcaa atcgttttat tattttaagc cagggaaggg ctgtttttga
     4621 cggaagcgtc gaagagggcc tttccgaaca aatgctcaca aagctcgaaa actggggcat
     4681 tcgaaacccc ttgcaatcgt acaaaaagaa agaggattta cattggtagt aaatcttttt
     4741 tcataccgta aagcagagag ccttttacat aaagcaaacc ccggcctaaa acttgttttt
     4801 ctcatacttt tttcaagcct tatttttcta aaacaaacat ggattgcacc gcttctttta
     4861 acagcgcttt ctttttgcct tgccctcctt ctttttttta tggcaaaaat accagtaaaa
     4921 aaaatctttc aattaaaact tcttgttttt ataggctttc ttttcgctct ttttaagtcc
     4981 ctttctttag acatccagtt ttcgcctcgg tttcaatttt tatgctcatt ctccttgcag
     5041 gatggcttag ctggcttgtt ttggggcttt aattttttta ccgcttcttt ttttgcactt
     5101 atcttttttg acacaacgag tatgagtgat ttgcaagata gctttcatag tgttcaaaaa
     5161 aaactctctc gttttattcc tattttagaa aaaataccct tggcaaatgc gcttgcctta
     5221 actatctctt ttatacctga agttttttcg ctctgggggc agataaaaaa agcatccctt
     5281 gcacgacaaa atacgcgcag ccatgttcta acaaaaccaa aaggggtttt caaaaaaaaa
     5341 tacaaaaaaa agctttctct acgccgacag cttgctgttt tcctccaaga attttctacc
     5401 cttatttccc tcctcctttt acgtgctatg gaaaaacgaa aagcattgct aaatcgctct
     5461 taaagcgata gtattacaat atatttacac gaaagaaaag gacataaagg aaaaagcatg
     5521 aaggcgctca ttgttggaaa tggaagttta gaaaacatag acctgttaaa aaaagaggca
     5581 aaaaaagcag actttattat ttgcgcagat ggtggcgcag gtttcctaga aaaagcttct
     5641 ctgctgccgc attttttaat cggagacttt gattcaatcg atcaaggact tctggacttt
     5701 taccgaagca aaaacgtaga aattatacag catccaacaa aaaaagattt tacggacacc
     5761 gaacttgcta tagagcttgc actagaaaaa aaagcgagag cgataagtct tattgcggta
     5821 accggaagtc gcatcgacca cagcctctca tcactttttt tgctcaagcc attaattgaa
     5881 aaaaaaatag acgcgaaaat tctaagcgat tgtaacgaag tctatctcct cgggcctggt
     5941 gaatcaagca tcaaattcga gaaaaaaaat ctttccctaa acatggacga actcatcctc
     6001 agcctcctag cgctcagtga aaaagtcgaa ggcataagaa cgacggggct cagctatccg
     6061 ctcgtggatg cctgcctaaa gcaaggctca agcctaggag taagcaaccg tttttctgcc
     6121 ccttctgcca caatcaggat aaaaaaaggt tttctgtata taatactatc aaaagagata
     6181 taagaaaaga taaggatcca ctatgaaata cagctttaaa aatgattatg ccgagggtgc
     6241 tcaccctcaa attcttcaag cgcttgctga aacaaacatg cttcaaagcg atggctatgg
     6301 gctagactct ttttccgagc acgcaaaatc gattctgtat aaacatatta actttcctca
     6361 agctcaagtt cattttttag tcggtgggac acaaacaaac ctcatcgccc tatcttcttt
     6421 tttacgtccg cacgaagcag ttatcgccgt ttcttcaggg cacattctag ttcacgaaac
     6481 aggagctata gaagcaacag gacataaaat catatcaacc aagggcaaag aagggaaaat
     6541 atacattccc gaactagagg ctctcttaaa agaacatagt gatgaacata tggtaaaacc
     6601 tcgacttgtc tacatatcaa atgcaagtga aataggtacg atatacagca aggaagaact
     6661 tgagactctg tatcgttttt gtaaagatca gaatctttta ctttacatgg atggcgctcg
     6721 cctcggtgca gcattgacag cgcatggaaa taatctttca ctttctgata tcgctgcctc
     6781 ggtcgacgca ttttacatcg gcggtacaaa aaatggagca ctctttggag aagccctcgt
     6841 catttccaat ccttcactgc aaaaagactt tcgtttctta ataaaacaaa aaggcggcct
     6901 ccttgcaaaa ggacgcttac tcgccattca gtttttgacc ttgtttcaag acaatttgtt
     6961 ttttgattta gcaaaacatg gaaatacaat ggctgggaaa atacaaaaag aactaaaaga
     7021 cttgggcttt tcgtttttta ttgaaagtcc tacaaatcaa atctttccta ttttccccaa
     7081 aactcttatt gaagagttgg aaaaagacta tggcttttat gtctgggaag atatttcggc
     7141 aagcgaagct gctgttcgcc ttgtaacttc atgggcaacg aaagaagatg cggttaaaag
     7201 ttttataaaa gcggtggaaa aatctagcac gtacaacctt aaaaaataaa tatacaagga
     7261 aggaatctat gaaaaaaaag aacgttttta ttgtctattg tcatccctca gaggagtcct
     7321 ttacccgaaa tgtccgagat tcttttatac agggcttaga agctgcgggg catagctatg
     7381 aactttccga cctctacgca atggattttc aaagtgatat gagcaaagaa gaatatacga
     7441 gggacgcatt ttatcatgat attcccgtaa ttggcgagga tgtgaaagaa gaacaagaaa
     7501 aaataaatag agctgacata atcgttttta tctatcccgt tttttggacc gatgtgccgg
     7561 caaagatgaa gggttggttt gatcgcgttc taagctacaa ctttgcctac ggaaacaaga
     7621 caatgaaaat gctcgataag gtcctcatcc tctgcaccgc aggcaataaa atctggcatc
     7681 taaaacgctt tggcttattg cgaagtatga aaaaaataat gctcggcgac aggtttttca
     7741 atcgtgccaa gaaaaaggac ttcgtggtct ttgacggcat ggcaatgagt tttcctgaac
     7801 accgtgaaaa acactgggaa atttttaaga aaaaagctta cgaaataggc aaaaatctat
     7861 aaacaataaa accgagcact ttatcgctat cgcgtcttta ttgtttacga tgagtacaaa
     7921 ttacttttca caaaaaccta tctctttaac aatgaaaaga aaatttaagt ttctgcataa
     7981 cgttctaatt tattaagcca tttttctcgt atctcttgtt tttgcttaag aacgtttgga
     8041 aaacttatcc atttgatatt ttgcactcca caagatttca atgtacccaa gatcatagag
     8101 tttttcaaag gtgattttaa gaaaagatta taataaaact gtggcgaatt catagtcgaa
     8161 ataacggtag ctttttttat atgggtgagc ttcccttgga gcaagccact ggcggcagtt
     8221 tcatatgccc aatggggaag aaaaactttg tcgaaaaaac cctttagaat tgctggaggc
     8281 ataccccacc aaattggggt aataataata atttcctctg cccgcatcaa tttttcttgg
     8341 tattcgccta cttttgggtc aacaaaagca cccttcctat attccgcaag atctgcttct
     8401 gtcatgacgg gattaaaacc atctaaaact aaatcgagaa tttcatatga aaccttgcgt
     8461 ttttcaagcc cctttgtaat cgccttcaaa atagcaaaat tatagctccc ttcccaggga
     8521 tgacaataca aaattaaagt attcatttgg tttatttcac ataaaggaaa aaaaatgata
     8581 gaaagcatac ttatagaagg tttaatttac ggcatcatgg ttttgggggt ttttataaca
     8641 ttccgcattt tggattttgc cgacctcacc gtagatggct ccttccccat cggtgcatct
     8701 atcatgggaa tttttttgct caagggagta aatcccttcc tcgctctcct tgttgctttt
     8761 ttaggtggcc ttatctgtgg tcttatcacg gcccttattc atacaaaact caaaatcccc
     8821 ggcctccttg ccggtatttt aactatgaca atggtctatt ccataagcct gcgcataatg
     8881 tctaacaggg caaatatttc ctatctcaaa gtacccaccc tcttttcaaa aattcaagcc
     8941 atgagcgcag cttttttaag cccggaccta agcatacttt tttttagcat acttgttgtt
     9001 gcggttattt tggtattatt gaacaatttt tttacaaccg actttgggct ttgcatggga
     9061 gcacttggct caaacccaca aatgattatc tcccaaggca tgaacccaga gattatcaag
     9121 gcagttggca tagctctttc aaatgggctt gtcgcttttt ctggcgccct cgcttcaatg
     9181 tatcagggct ttgcagacgt cggctctggc tcaggcatcg caatcgcagg acttgcctcc
     9241 ctcatgattg gcgaatttat tattcgctca aataaaatct ccgcccttac ctttcgcgtc
     9301 atacttggct caattctcta ccgagcactc atgtatcttg cacgaaaata cggctatctc
     9361 attcacatga acccaaacga cctaaaactc attaccgggc ttttaattat tctctctatg
     9421 gtaatctcaa aaacaaagtt tagaaaaaaa cctagcgtac acaaggcgga ggcatctcat
     9481 gcttaaactc gaaaacattt caaaaatatt tgcagaagga agccccgacg aaaacagggc
     9541 gcttaaaaat atcaatctag aaattcacga agaagatttt gttaccgtca taggctcgaa
     9601 cggagcagga aaatccaccc tctacaatgt cattgcaggt acgatgaaag cgacaagtgg
     9661 cattgtaagc ctcaagacaa aaaacgggat aaaaaatata acagaagata aagaacatat
     9721 ccgtgctcat tatattgggc gcatttttca aaacccgctt ttgggtaccg ctgggaaaat
     9781 gtcactcgaa gacaatatgg taatctgcag caaaaaaggc tataagggct taaaaaaaag
     9841 cttaaacaat aaaactcgag attactttag aagccaactc gaaatacttg gaatgggctt
     9901 ggaaaatcga ctcaaggaca atgttgagct tttttctggc ggtcaacggc aagcgctcac
     9961 cctcctcatg acccttatgt caaagccctc tcttttactc ctcgacgaac acaccgcagc
    10021 cctcgaccca catatggctc aactcgttat ggacctcacc aaaaaatttg caagcgacga
    10081 caaactcacc gtcatgatga taacgcacaa tatgaaccac gcactttcct atgggaatcg
    10141 ccttctcatg atggacgggg gagaaatcgt cctcgacatt ggtgcagaag aaaaagcaaa
    10201 actgagcatg gcagaaataa tttcacagtt caaagctatt aggaaaaaag aaatagtgaa
    10261 cgaccagatg ttgctacagt aagacgtatt ttatgcaagt cgctttcaaa ctgacaatag
    10321 actttttatc tgtccatgat agttattatc gggataacaa cgcatccccg atggtaaaga
    10381 gctagaaact cttataggaa caatacaagc atatatcaaa aaaaacattg ctattattcc
    10441 gatagtatga tatgctataa aaaaagctgt ggagtaaaac catgcaatat atcacagtaa
    10501 aacaagcctc ggagctttgg ggcatatccg atcgccgtgt tagactactt tgtgcagagg
    10561 gacgaataga gggacttatc caaaaaggac gctcttatct aataccgaaa aacaccttaa
    10621 agcctcttga cgggcgcagt ctgcgcggta aaaatattcc agaacaatac gctcgcttat
    10681 ttacgaccat tgatgaaatg aaggcagagc ttaaccggcg acgtcctctg agcatcggag
    10741 aattgaaacg cttgcaagat gaattccttg tggaatttac ttataactcc aacgccatcg
    10801 agggcaacac gctcacactg caagaaaccg cactggctct ggaaggcgtg acaattgata
    10861 gaaagccact gaaagatcat ctagaagcag tagggcatcg cgatgccttc ctttatgtcg
    10921 cttcccttgt cagcaataaa gttgcgattt cagaacggat tatccgtgaa attcactccc
    10981 tcgtcctgat aaacaaaccg gaggacaagg gcatatatcg aagaattcct gtaaaaatta
    11041 tgggggcaca tcacgaacct ccacaaccct atcttgtccc tgtgcagatg gaaaaattac
    11101 ttacaggctt gtcccgtgtt gaccaacaca ttatagaaac agcagcactt ttccaccttg
    11161 acttcgaggg catccatccc ttcgtcgacg gcaatgggcg cactggccgc ttgattataa
    11221 acctgatgct tatgcaagca ggctatccac ccatcgatat gaaatttacc gacagaagaa
    11281 aatactattc ctgctttgac agttattatc gggacaagga cgcatccccg atggtaaaga
    11341 tgattggtga atatgtaaaa gaaagacttt cgcactattt aagtttagta gaagattttc
    11401 atcgacccat gtaagcaagc atgaattaga tttttgagtt attaggcaaa aagaggacag
    11461 gcatatcgta aaatacgaag cagatttttc ataccttcat tgtaaataca tgaagcattt
    11521 tatgcaagaa gtgaaaaaag atttgctata agattttgag accccgtcta ccttctaact
    11581 aaagtaaaaa aaattgctct ggagtgtagg cgggaattcg agaaagcttg aaatcagaaa
    11641 tatttctaga aagaatataa tcaacttgtt ctttaatcgc actgacttca attactgcat
    11701 cttcaaaatc ctgaattggt gagataagag cttctttcaa aatattttta tcaagtgcaa
    11761 ttactttaca caagtcgagc aagctcgcaa gtgtttgcat tatttttgaa cgatctcttt
    11821 tttctttaaa taaaaaatag gataaggtag taatttcatg agcacaaaca aaaccttcta
    11881 cttctttttt tacacactta tccataagaa ttgctgcctc tttatggtaa tttcttttat
    11941 ttagaaaatc caaaataata ttgagatcaa tgagtatctt tttcataaaa acccttacgc
    12001 aattcttctt tatttggaat aggatctctt tcaaaagccc cgtaaagtag ttgcgttttt
    12061 gaatcaagct cttgtttttc acccttagat tgtagttgaa gcagatattg ttcaaaaagt
    12121 tttgatactg aaatatcatt atgtcgagaa tatgtatgag caaaatgtat gaggtctttg
    12181 tcaagactaa gcgttaattt tttcactatt cttcctcctc tattcattct ttttgggttt
    12241 ctatacgtat aaaatataac acgatacgta tagaaaaaca agattcatct caagcaaaaa
    12301 acctctcgtc gactggttct attgcatctt ttagatttac atggcagtag gcaaagggtc
    12361 tccgctctat atatctttga tgatattctt ctgcaggata aaaaaccttt gcttctaaaa
    12421 cttctgtaag aatggggtcg taatactttt gtatctttgt tagatgctct atcattgcat
    12481 cgatttcttt tttttgccct tcttttgtat aaaacacagc agaccgatat tgtgtaccaa
    12541 tgtcatttcc ttgacggtta aggctcgttg gatcatgaat agaaaagaac cgagcgagga
    12601 gctctttaaa ctcaagctta tttgaatcat agactacacg cactgtttct gcatgccccg
    12661 tttctccatc gacgacagct atgtaattcg gattttctgt atgaccgccc atatatccag
    12721 agcttgactc aataactccg tgaacgcgtc ggaagtattc ttcaactccc caaaaacagc
    12781 ctgctgcaaa atatattgtt tgcttagaat tttcttttgt catccttacc ccaatctctc
    12841 tctatgcttt tgataaaagc cacaacaaag tctatggctt ccttcccttg ataattatgg
    12901 tccttgtttt taaactcgcg tatttcaacc cgtccttctt tttgcaaagc ctgtgcatag
    12961 gcgaggcctt cgtcatacaa tgggtctttt ccgcaaagta caaaagctgc aggaggaagc
    13021 cctgaaaaat cttttgtcat aaggggaaag acataggggt tgtaaagatt ttcctcaagc
    13081 ttgccttcaa gataaaagtc atagaaaacc tcaaggcttc tttctgtgag aaaacttttc
    13141 ttttgatttt ttattcggct ctcccaagcg tcgcctgttt ttgggtttat ttttgcggtt
    13201 gccggtgaaa ataaaatctg cgcaaggggt attttttccc ccttatctcg ttggtattgg
    13261 caaataacag cagaaagact tgcgccagcg ctatctcctg caacaataac atccctatac
    13321 tggcagtttt ccaaactgct atttgcaata aaataattat ataccgcaag gcagtccatt
    13381 aaaccttccg gatagggata ctctggtgca agggcataat ttatggacac aacaatacaa
    13441 tgcgcctttt gtgaaatttg ttttgttaca agatgctttt gcgcataggt tcccaagaca
    13501 aaacctcccc cgtgcatgta taaaacaagg ggtgcatcct catttaattc cttggggata
    13561 tacacacgaa cgggaataga atgcgtttct gtttctataa ctgtatcaaa aaaggtatct
    13621 tttttctttg taacaagatt accgatcaat tcattcatag ttcgtatact tttgtatttc
    13681 gtatcaacat tgtccatata atggcgttga atcgaagtac aagaaaaaaa gagaaaaagg
    13741 cataaaagta tcaatagtat aattcgaaaa tccatagtac aattgtacat tgaatagaat
    13801 gcttaggcaa gaattatatt tgatttggta ggagactatg ggaaaattaa aaaaacaaat
    13861 gcatcaacga cctcgttata aagatttcgt atttgccctt ctttttggat taggagtttt
    13921 cttgctcatt attggattaa aagacggtgg ttttctttca gtctttcgca aagcggcctt
    13981 tatttgcctc gaatgcatcg ggattggata ggagcaaaaa tgaaaaaata caactttttc
    14041 acacgaattt acatacaaat cgctgctgtt tttgcctcaa atcctttttt acaaaacttt
    14101 tccaagggta ctatttatca aggaaaagga aagggtatct gtgttcccgg attaaactgt
    14161 tactcctgcc ctgctgcagt tggcgcctgc cccttaggta gcttacagtc tagcttggca
    14221 agcccacaag gaagaacaag cttttatatc gcaggctttt tagtttctat tggcgctttt
    14281 ttcggtcgca cgatttgctc ttttttatgc cccttcggct ttttgcaaga actcttatat
    14341 aagataccta caaaaaaaat aaaaaaatca aaaacaacga gacgcctttc catagcaaaa
    14401 tatattttcc ttgtcgtttt tgtccttgcg cttccctatg gagtctatct tgcaaccggc
    14461 atcgggagcc cttttttttg taagctcatt tgtcccgcag gcagcttgga ggcaggcatc
    14521 cccctcgtta ttgcaaacga aagcttacaa tccctgattg gctttttatt tagctggaag
    14581 atgagcctcc tcgcgctttt tattatcact tccattttta tttaccgtcc cttttgccga
    14641 tttgtctgtc ccctcggtgc tatctattca ttttttaaca agtacgcact ctttggcgtt
    14701 cgagtcgata ccgcaaaatg tataagctgc aaaaaatgca tcaatttttg caagctcgat
    14761 gtccacgaag taaacgacag agaatgtatc cgttgtggag agtgcatctc tgtttgtcca
    14821 acagaagcaa ttagtttttt tcaaaaaaga acaaataata atacaatatc ttaaaggagt
    14881 tactatgaca aaaaaaatac taacactgag ctttgctttt ttaattctca taagctttgt
    14941 ctttacaagt tgtgcaaaga atgaagaaaa agaatttgca aaagctgccg agcaacttaa
    15001 aaaataccaa atggtagaaa aaaaagaaag cgagccgaca acacaagaag tcaaaactga
    15061 agcaaaaaaa gaaggaccca tggctctcga cttcagcctt caaatgacag atggaacaaa
    15121 atttactctt ttgcaaaatc ttgacaaacc catcctcgtc aattattggg caacctggtg
    15181 tcctccctgc gttcgagaat ttcccgacct tgaagagatg tataaaaact ataaagatag
    15241 aatgcacttt gttgcaataa actctgcaga aacaaaggat gtcatcgaag cctttatgac
    15301 aaaaaacaaa tacacgatac cagttgcaat ggatagcgac aatgctgttg gtatgatgta
    15361 cggtatagca agcattccaa caacctttat cgttgacacc gagggcagaa ttgtttttgc
    15421 gcaactgggt atgatgacaa aaaaacaaat gcaaaacgca attgaagctg ctttataaac
    15481 attaaaacac aaaagatttt cgaataagtt ttactaaact agcccctccg acggaaagtt
    15541 ggacgggctc ttttttttct tctacttcaa gatgtacaag agcatcattt gtaaattttt
    15601 ctatcaatag aggtttccct acaaaaacct ttttctcttc tttttctaag ccgtcatagc
    15661 gaatttcaag tcgaagttcg gaggaaattg cttgctcgac gattgctatc ttcccaagaa
    15721 aatccatacc ctttgcttcg gtttttacat atcgcactga gtccggacga atttgctccg
    15781 tagaaaaaac aatttttttc ttgatgcgta ggcttaaatc ttctctttgc tcttttgtaa
    15841 ggtccatctc tgctaaataa tccaacaatt ctttttgtac atctcgtttt ttggaaagaa
    15901 gaatgtgatt tttttcttca tctgtttttg tatctgcaaa atcgattggc gattgaataa
    15961 gtgcatgttg cagaggactt agaggaaggc tgatactcgg ctttgcaagt gtctttatgc
    16021 gcccaataaa atctaagcca cttttttgta ccgcatcatg cgcttcgtct tcatttgcaa
    16081 aattcatgag atacacaccg ggtgccaaag tgtggcaaag atatttttta agaacagcat
    16141 tattttccac agcaattatt ttatcagcat caacatgaag aacaaaaccc ttgtacaaag
    16201 aacccgactt aaaattggca taccagtctt gtatcgcatg gtgaaagttt tgtggtatct
    16261 cgtgaacgga ataggcttca aaggttttca aaataaaatc tggcttcatg cctgattcga
    16321 aggccctcgt taccgattgc tttgttattt caaaacttaa acttgtatca caaccttgca
    16381 agtccataat ttttacaagg ggtaataaag aatgcaaaga caaaccggga aggatagaaa
    16441 caaaaaagct tgaaccgaga tggacgagtt ttttcgtatc gctactcgtt tcggttttcc
    16501 gaactttgta tagcgcttca tcttcatcat cgatgccaag agcttctaag agtccaaatg
    16561 aaataaggct atccacaagg agcgcgctgt caaaagcgag ctcggttttt ttagcttggt
    16621 tttgttgcaa aagcgttcga agctttgaaa ccttcggcat agtagtggca tgcagttttt
    16681 caaaaaacac aaaggcaatt cgttttaaaa tttttcttgt atatagattt tcttcaagag
    16741 acattaaaaa aacggtacaa atagaagcaa gatttgcaag aacagaacgt ggaagacttg
    16801 caattgacgc tgcagcaata taggcatatt gttcttgttc cgataaaagt gaaaattcat
    16861 tccatctttc atgatcgata tgtgtttcat tttccacttg tttaaacaag cccatatttt
    16921 gcaatgcaga aataagtttt tcaaaacagc ttatatcgca atgatttaaa aataattctg
    16981 aaatcgtttg tgcatgtttt tttttcaagc ttccatctat tttacataaa tcggggaatt
    17041 gaaacacaaa ggcatagaca gcagcaagca aatgcgagtt gagaaaaaaa gtattttctg
    17101 aatctatgtc tgcatgcagc ttactctttg gaaataaaac gcttgaacca ataaaaggca
    17161 agagggcttc ttccaaaaga ggatttataa gatagatttt tttttctgta cttggctcaa
    17221 ggcttctaaa aagcaaaagc cgttcctcta agttttgcaa gtccgtctca aggtcataac
    17281 ttgaaatact gtcagaaaag aaatgtttta attgcgtaaa actcgctcct gaaaaaacat
    17341 gtaatgccgc aagaatgagc ctgtcttggt agtccaacaa agaaagtatt ttttgcttat
    17401 tttcttcttt gcgcaaaaca gaactcattt gctcgataag gttttgttta ttgtacggcg
    17461 tttttatttc accgatatac atacgaagca aatcaaaaaa ttgctggtca gaaagctttg
    17521 taaggctttc cctccagagc aaaacttttt ctgcctggct atgatttttt agattcatca
    17581 ctctctccat tttctgccaa atactgcgct atatcttctg gcgttctcaa aagcacatag
    17641 gcatacccct gctccgccaa aaacttttgt ctgtttgcac caaattcttc ttccacggta
    17701 ttccgtgtaa taatcgtaaa aaaccgcgag gtgcgttctt tgggccgcaa aattctaccc
    17761 aaacgttgcg cctcttcctg cctgctccca aaggttcccg aaatctgtat cgccatcgaa
    17821 gcatcaggta aatcgatagc aaagtttgca accttggaaa caaccaaaac actgtttttt
    17881 ccagatcgaa aatcagcgta cagtgcatcg cgttgtgaag ttggtgtttt tcccgttata
    17941 atcggcgcat tgagcaagac cgcaatctca tgtaattgtt gcaaatattg cccaatgatg
    18001 agaattttat catttttaaa atgccttact aaatccataa ccaagggaat cttcgaaggg
    18061 ttttcgcttg caagtctatg cttgagtcgt ggctgtactg tcgcgtattc gatttctttt
    18121 tcacttgcca aatcaatacg cagctcaatg cactcagcgc tcgctatcca accagcacct
    18181 tcaagttctt tccatggtac gtcatagcgc ttcgggccaa ccaaactaaa gacatgcccc
    18241 tcgcagccat cttcgcgaac caaggttgca gttaaaccaa ctcgacgaat agcctgtagt
    18301 tcagctgtta cccgaaaaac aggcgcaggc aataaatgca cctcgtcata tattatcaaa
    18361 ccccaagggc gttctttgaa aatttgaaaa tgtgggaagg cagagttttt atctggtctc
    18421 caagttaata cttgataagt tgcaactgtt accggtttta ttgttttttt ctcacccgta
    18481 tactctccta tttgctctgc agtcaactct gtcttatcga taagttcatc tatccattgg
    18541 tgaaccgcag aaatatttgt cgtaagtata agcgtgttag tgtgcagcat ggacataatt
    18601 ttcatgccga caattgtctt tccagaaccg cagggaagta caatcgtacc aaaacctgaa
    18661 cccggtccct tatttcccac aagcgcctga gccgcttcca cttgatactg gcgcggttca
    18721 aaggcgtgac cgaaaagtgt tttctcccga agcaaaactt ccagaggctc gccgtcgcgc
    18781 aagggaacct cgtcttttac cggccagcca agcttcatca aatcttgttt aatacttcct
    18841 cggtcgacaa gcttgagaga aaacgcataa gagctatcaa acgtatattc gtttgcattg
    18901 agcaggggag tcaaggtttt tctcagcgac ggactcgcct ctatttcccg gtatattgtt
    18961 tggctttcta cctttagaat aagccgttct tcatcttctg ggctcaatgg gtcagggagt
    19021 aaaataagtt taccgaagcg actcgttact tccgttaccc acgttaaaac agatgtgggc
    19081 acttcgtagc gagaaaactc catcaaaacc cttgcgacat cttcagggct aaaaccagaa
    19141 ctcctcgcat tccacaaaga caagggggta agacgatagg tatgtagatg ctcgggagat
    19201 ttttctagct ctgcaaaggg taaaagcgca tcacggcatt cagccgaacg aggcatatgc
    19261 acgtcgagaa gaagcgttct gtcactttgt acaattaatg gattttgact attcatattt
    19321 ttattttaaa aagcgaattg ctccttcatt ttgatcttga agcaatagcc accgagtagc
    19381 atctttttgt aatttttttt caagtttatc actttccaaa agcgaaaata aaagcgaagc
    19441 tattttttca tcatctttta aagtcgtcct tgtcaaaaca acagagtttt ccagtttctg
    19501 gctatatcga taggctgtca tttctgcaaa agcaacaaag gcttcttggg cagcatacaa
    19561 agcgggattg ttgacctcaa cagaattttt tgaagagcga aagagcggaa gtgcttcttt
    19621 aagtagaaaa ataaaaccac cctgtttttt ttctttaaag cgttccaaaa tattttccgt
    19681 tatatattga tatgaagcaa tgagttcgtc agcaataatt tgaaaatgat ttttccccaa
    19741 ctgagaatac tcttctgtaa aatgatcata atcaaaatac aaaagaacac ggtcgataga
    19801 gtcaagaagg gtttcagctc gaatgataag attcatggaa gaaagattgg aagccctatt
    19861 ccacggaata atcgactttg tcccctcaaa tacagtctct ttgccctctg actttgcctg
    19921 cgcctttgta gtacagacaa aaggaatttc ctttcccttg caataagcta caaaagcctc
    19981 gttttcgaga aaatctttcc ctatcaaaag aatattgttt ttcatcttcc taccaatata
    20041 acacaagaaa ggtttatttt caatgaacaa agcttgacga ttatgcattg tttcgttatc
    20101 ctaaaaagca tgaaaatatg gattaaatat gttttagcga gtattttggg ttcacttgct
    20161 gccttcctcc tccctttaaa tatcacaagt gttcaaaaaa caattgattt tttaaccgaa
    20221 atatctatcc gctttggtcg atatatgctc gttccgcttc tttttttcag catggctctt
    20281 gctatctata agctgcgaaa cacgggaaaa ctttttaaaa cagctgttta tgtcgcaacg
    20341 gttagcctta tcacttcttt tgtccttgtt tttttaggaa ttctcaccac attattgata
    20401 aaactccctc gaatccccat ttctgtggaa aaaatgacaa attctgcgag tttggacatt
    20461 cccacccgct tactcgatat tttccccatt actagttttt ccgttttttt agatggaaac
    20521 tttttattgc ccttacttgt tttagcaggc ttcatcggag caggttgtgc aagcgacaca
    20581 aatgcttcaa aactcacagt aaacctcttt gactcacttt caaaagtatt ttttgccgtt
    20641 atgagcttct ttgtcgatat gtttgccttt ggaatgattt ttatcacctg cacctggacc
    20701 atactcttca aagacttgct gcgatctggc gtttttaccc cactcatttt attgcttgca
    20761 ggggaattca tcctcatcgc cttcatcctc tatccactcg ttttaaagtt tgttttcaag
    20821 gaaaaaacac cctaccgagt actctatgca agcaccgcaa gccttataac cgcctttttc
    20881 tcaggcgaca taaacctcag cctcggcgta gcaataaaac acctcaaaga aagccttggc
    20941 ttaaaacgca gaattgccgc cgtaacagcg cccgtttttt cagtctttgc gcgcggagga
    21001 acagccctcg ttaccgcaac agccttcatc gttattttgc gctcatattc tggactcgtc
    21061 attccctttg gagaaattgt ctggattggt tttatctcct tcctcatctc ctttttcctc
    21121 ggcggcattc ccattggcgg cgccttttta tccctaacca ttctttgctc tttctacggc
    21181 cgcggctttg acgcagccta tctcttactc aaaccaatgg cctttgtcct ggggtctttt
    21241 gcaaccgcaa ttgatacaag cacgatgatg tt
//
LOCUS       DAOSSW010000024        20051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_2867_length_20051_cov_7.392078, whole genome shotgun sequence.
ACCESSION   DAOSSW010000024 DAOSSW010000000
VERSION     DAOSSW010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 20051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20051
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2867_length_20051_cov_7.392078"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /gene="rlmD"
                     /locus_tag="PKW26_03520"
     CDS             complement(<1..1478)
                     /gene="rlmD"
                     /locus_tag="PKW26_03520"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOQ92670.1"
                     /translation="MKKNESIGTGPNIKTIDLNSEAHAVAKINGKTIFIEPMKVNTFS
                     KSNTENQLLQDEHYGALPDEIIDCKIIADKKNFFLGELISINTKSTKRIKPQCPIFGL
                     CGGCSLQHLSYDAQLAFKQNLVLQNLKRLGNFNLIENNIKIENIIGMENPWQYRNKAE
                     FPIGSIFDSTFNLTADSKTDSQNLHAGFYKKKTHEIISTENCLIQNTLSNTARLVLKD
                     LFPKLCHLVTRISKNNSLMLILVTDDLRFFNTIKTNTNSSIKQSFTDESFTAKSFTEK
                     CSALQKAIPELTSLYLSYSPNASHSNGKDFIQLFGDAFLIDSIGSLSFEISPQSFFQT
                     NHIQTEQLYKIVKQYASLSGNETVVDLYCGIGTIGLYLASHAQKIIGVESVKEAVENA
                     KANAKRNSIPNAQFIHSRAEHALEKISEPIDLLIVDPPRAGLEEKLIKEIIKKNPKKI
                     IYVSCNPASFSRDIKRLREKSSENYSLTKLQAIDMFPHTTHV"
     gene            1549..2787
                     /locus_tag="PKW26_03525"
     CDS             1549..2787
                     /locus_tag="PKW26_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOQ92671.1"
                     /translation="MDFIQLTQVSETLLTLCIMLFAGFLLTRLTKMAKLPNVTGYILA
                     GVLIGPSVLNIIPKQIVANMSFVSDIALTFIAFSVGRFFKKEKLKKTGGEVLVITLFE
                     SLIAGIVVTFVMFFIFKLSFAFSLLLGAIATATAPASTIVTIRQYGARGKFVNTLLQV
                     VALDDAICLIIFSIATALVGHEAGQAASLQSILLTLLFNILSVGLGFLCGFFLSRLMT
                     PTRSEDNRLILTLALLLGIAGLCALVHVSPLLACMAFGMAYINLTKDKVLYEQVDRFA
                     PPILSLFFIISGMNLDLASFASYGLIGVVYFIVRIIGKYSGAWLGCAVTKTSHKIRNY
                     LGFALIPQAGVAIGLAFLGQRLLPEDSGIMLLSIILSSSVLYELVGPASVKFALHRSG
                     AIEKGQKKKREKEEKAKEKK"
     gene            2908..4407
                     /locus_tag="PKW26_03530"
     CDS             2908..4407
                     /locus_tag="PKW26_03530"
                     /inference="COORDINATES: protein motif:HMM:NF023283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 family protein"
                     /protein_id="HOQ92672.1"
                     /translation="MKNSSLEYFTHFLKTDSGANLLRAKSAAFVITFLYNEFRVDHVP
                     FILADDFESHLVSFLQNNVIDVETLLISEEEAPQEVLLGDIQAKAKFLVSYWCSENKN
                     YIKRYYSHNKNFVIELHPSIERLFTWLESCEPEEFVGTESRFQDILFQLRNLKTNMDQ
                     NPELRIAELKKEKKRIDDEIQRIKESGRAETFTPVQIQERLLNISRSSRELLGDFRQV
                     GENFKQILNDIYREQTETNATKGEILGYTLDTNRQLKLSPQGQSFSTFWKFISQDSDN
                     EINTLIESIIISQADRGLVYSDVFLSRLKHYLYQEGHKIIEQNRQLTDKINRVLSRQE
                     QDEYQRVKELSNEIKSLVKALFSKEKKLPEQCAMEIEGKAEIYAPQARYPVLKSEETI
                     FTAMESFDSRQINQEALEDVFNQFYIDEKLLLSYADEFRKKMNGKQFTLAEMLISYPI
                     EKGLAEIITWFSIAENDTRIIIQKEKNDSIEYNKNGDLIKVKLPRMVFS"
     gene            4404..5033
                     /locus_tag="PKW26_03535"
     CDS             4404..5033
                     /locus_tag="PKW26_03535"
                     /inference="COORDINATES: protein motif:HMM:NF025205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HOQ92673.1"
                     /translation="MSQKESSPPWAPVCIKLLQGPLYRANAKDIYWDLLISHESHIQS
                     YFATLGVSVLVDKSDGYAFLSQIDDEQIDSNSLPKLIRRIPLTPEISLLCVLLREALD
                     SFDSAQSDSSVLVMTAHEIKDLLALYIKEQSDQTKFITKLDSYLTQLVGFTFLKELAP
                     ENGGVLSGLERIFEVRRIIRAKIDAEFLEEFKRKLSEEAEKIETEQEEV"
     gene            5033..>5235
                     /locus_tag="PKW26_03540"
     CDS             5033..>5235
                     /locus_tag="PKW26_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ92674.1"
                     /translation="MANELFSSGGYRLDVFQLYNWGLFDKVIYSLDCAQGTTLLTGAN
                     GSGKTTIVDGLLSLLVPPQQRHY"
     assembly_gap    5236..5335
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5336..>6852
                     /locus_tag="PKW26_03545"
     CDS             <5336..>6852
                     /locus_tag="PKW26_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92675.1"
                     /translation="IEQINKILSEEESSFDKQGRWKRILSEKYNTVFFGDSFKKYSDF
                     YSSLFGFKSDKALRLFSQIVGLKVLGNLTEFIRTNMLEETDVYAEFEKLDTNYIKLMQ
                     SNNEIQKTKKILELLEPILELGKKLEKEKSEKNRLETISSLVPLWKAEKAIFLLTENK
                     IILERNKADLESQLSQNRKEKSETDNKISSLQISLATNDTQSRIETIDREINIKTDEK
                     KRRDLKRSQYEKMANEVGISIPTNETMFRNNFEKIVEKEKAEIELKKTNEDIRFNNQT
                     EFNTLQNEAEIIKNELASLSKRNTNIPDANIKIRDSICQKINCKADEIPFAGELIQVA
                     SNEQNWNYAIEKLLHSFALTLLVPEKLYKKVTNYVKENNMKGRVVYFKIEEDVFSLES
                     KIQLQKDSDTVPGKLEIKENVWVRDWLVDYIAKQFDYRCTDNIEEFSRAHKAVSSTGL
                     IKNKMRHEKDDRQFSDEKKLKVLGWDNKEKKNILSSRYDEIESLQLKQTTLLNEI"
     assembly_gap    6853..6952
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6953..7782
                     /locus_tag="PKW26_03550"
     CDS             <6953..7782
                     /locus_tag="PKW26_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOQ92676.1"
                     /translation="LYEKLKKDDLPKYQEQFHEYLQDTMNNDIVDFNQFIQNEEQEIR
                     TAISDLNASLRKIRYEHNPDTYLQLEFSKNNDVRINEFKEHLKNAIPNAFDLSSFDSE
                     KEEELFNKIKLFLDTVKANDANRKYILDIRNWYTFAASEKYKETGIQKQFYPDSGSLS
                     GGEKAKLTYTILASAIAYQFGLTQEESARSFRFAIIDEAFSKSDSANSDYAMRLFKQL
                     DLQIMVITPQDKINIVEDYISTVHITEKIGTKDSRLLPLTIERYKEQQKEVLQGKKI"
     gene            7785..9044
                     /locus_tag="PKW26_03555"
     CDS             7785..9044
                     /locus_tag="PKW26_03555"
                     /inference="COORDINATES: protein motif:HMM:NF021498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2220 family protein"
                     /protein_id="HOQ92677.1"
                     /translation="MITVKEIKQKALNRYSSFLKYKIVLELESEDTKIQNALGENGFD
                     SKRDSFFPLEIQGNKGNPNDDFKKRSAALQELIKASNLKKLFSYALETKDVLTRNNGT
                     QTIIQKIYFENEKSFLVFIEKELEVKRFCEALKIINNAKLKNFEMQTWAEKNIKALCT
                     ETEEHFWQNIVLVIAWLKDNPKSDLYIREIPLPIHTKFIENNKKLIESFFYTANNQSN
                     KKPSFEKLLGLKSNPLLFYFRFLDPSFSFVEGMHSPQLMAISLSHFNDFLETEKLKQI
                     QTVFITENKMVCLAFPKVKNALCLLGHGYYLLNLKNCSALQNKQIFYLGDIDEHGFDI
                     LSKFRNYFPHTISLCMDKKTYETFKFFAVSSDKKLPDNSIPKNLRDDELEIFNHLRKE
                     PEHNRLEQERITQDYIISQLKKKNILL"
     gene            complement(9039..10373)
                     /locus_tag="PKW26_03560"
     CDS             complement(9039..10373)
                     /locus_tag="PKW26_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ92678.1"
                     /translation="MEEKKLSLKTKLGFGVCDMGGNLFFTAMGFWSLYYLTDVVSLLP
                     AAAGFAIMIGKFWDAVTDPAMGYISDRTKHKMGRRRPYILYGSIPLFLSMWFFFTNPH
                     LESQTLLTVWAVLALCFLNTAFTIVNVPYSSLTPELTKDYHEQSALNGYRFTFAIIGT
                     IIGAAIVLPILGLFSTESKGFSAAGFIMGLVMLITALITTFSVKENPPALESIPKEGF
                     FKTYSAVFKNKAYLFLVGVFALHILAINLLQGILVYYFEYVLIAKEQTQIALIVLLVI
                     AMISIGPSVSLSKKLGKKLTYQVGLGLMALGCMAIFFFGHIFGFVFVFIIMAFVGIGL
                     GIVFATPWAMVPDTVEWDAIKTGVRKEGIYYGMWTFFSKVGQALSIGLLGLLLTMSGY
                     LPNIEQNNKTIFTIRLLVGPIPALIFLAAIITLIFYPISEKVYNEMMQKKES"
     gene            complement(10411..11757)
                     /gene="mgtE"
                     /locus_tag="PKW26_03565"
     CDS             complement(10411..11757)
                     /gene="mgtE"
                     /locus_tag="PKW26_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOQ92679.1"
                     /translation="MLINVITPEIQELINSHDWKSLKEVLPGLEPVDTSDLIRSVSST
                     EDRVIIFRLLPRDLAADVFSEMDWDLQEDILSGMNNTQIRQILTELEPDDRTRFFEEC
                     PSEVTRKLIEFLPVDERKKTLELLGYPEYSVGRLMTPNYVAIKKNWTIQRAMDHIRKV
                     GKNVENFDIVYVIDNQWKLLDAIELRSLILSDDDALVESIMDNTYVSLSAFEDQEKAV
                     YYLEHYGLPILPVVDSEGVLLGSITFDDILEIAKEEATEDFHKTASVSPLETPYMQTK
                     VLDFFKKRVGWLIALIFVNVISGFAMAHFEELIQAVTALVFFIPLIIDSGGNAGSQSS
                     AMIIRAFATGEVHLKQWWEVVLKDLLVSMMLGISMGFAVSFLGIYRGGILMGLSVALS
                     MAAVVITGSLLGTILPFVLRVFKLDPATASSPLITSMADILGIIFFFSISQAIMGL"
     gene            11919..12431
                     /locus_tag="PKW26_03570"
     CDS             11919..12431
                     /locus_tag="PKW26_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92680.1"
                     /translation="MIEKALLEYLQPVLQKNTQLFSLSCPEWILSKKRFLLGKEYVFS
                     IMLVLHTDLIPMEILFQELDSLFKEIGLDPKLKKKIQWFVLSPRGGIYLYESIAIREH
                     KKNKKMRNRFIIINTENASVSFGPGHKKNTTASQKLFSLIQNSRFFRREEWLSKMFDF
                     FSICEDTINK"
     gene            12524..13087
                     /locus_tag="PKW26_03575"
     CDS             12524..13087
                     /locus_tag="PKW26_03575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOQ92681.1"
                     /translation="MEQVFILSCIIQNLQIKPMVSLFQNIFYRYKKDNLEYTVENAIK
                     SFEQMRKNVEKQLDEEQAFFLEKISELVTYYGKDEAGIELRLPNILAQRLIEAVSNTA
                     IIITHPKRKKIKFLQQRKKDSEDYTIDFRNNEYSSETTTSLFLTISAGISCFSPKDTS
                     YLECFKRTDAALYESKNTGKNKYTILY"
     gene            complement(13136..14284)
                     /locus_tag="PKW26_03580"
     CDS             complement(13136..14284)
                     /locus_tag="PKW26_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOQ92682.1"
                     /translation="MNETNNTSDNASFEALLEQSLPNQKRLEPGQKIKAEVVSFSKEY
                     VFIHLNGKSEGIIEKKEFLDDEENLRIKEGDFVEAHFIAAKNGELFFSTKIAQEEASL
                     QMLENAYRASIPVEGKIEKEIKGGFEVSIGKHAAFCPFSQLGIAKEEQENIIGKVLTF
                     KIITYKDGGRSLTLSHLAVLEEERKNALKELSKTLSVGQKVSVEVKSLHPFGAFVDLK
                     GIKALLPISEIRFDRIDKIEDFLSLGETIEVAILNLDWENDKISVSKKALLGDPWENL
                     EDFAPESIHTGTITRIAGFGVFVRLKEGLEGLLHNSEIRKDERGNRDINLKKGDTIEV
                     IIKSVDSYNKKIALKQGETKEEIENAKEIKKHQNVDDAYNPFASLLKK"
     gene            14523..15977
                     /gene="gndA"
                     /locus_tag="PKW26_03585"
     CDS             14523..15977
                     /gene="gndA"
                     /locus_tag="PKW26_03585"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HOQ92683.1"
                     /translation="MGQKYSTIGLIGLAVMGKNLAFNMADKGFSVSVYNRSFDKTKDV
                     FAEYTSESFRGFEKLEDFVLSLESPRKIMLMVKAGEAVDQTIALLLPLLSPGDLIIDG
                     GNSHFKDTQRRTKALEEKGLLFLGTGVSGGEEGARFGPAIMVGGSETAYSLVENIFSA
                     ISAKYIGQDGKSEDCCGYLGSDGAGHFVKMVHNGIEYADMQLIAEAYSLLKEDFSNEA
                     LSQIFAEWNEGELESYLIDITKDIFKKKDAETGAHLVDLILDRAGQKGTGKWTAQISL
                     DLASPIQSITTAVYERFMSARKDERIRAASLYKGQAENGCESNFAASQKSDAEKKALV
                     EEVRRALYASKICAYAQGFSMLLDASREENWNLDFAQIAKIFRGGCIIRARFLTELAK
                     EFDAKADLENILLSPLFSATIKTYEKDWRSVVSKAILQGRSLPAFSSALSYFDSYTTK
                     NLPTNLLQAMRDYFGAHSYERIDKEGFFHTNWGE"
     gene            15977..17464
                     /gene="zwf"
                     /locus_tag="PKW26_03590"
     CDS             15977..17464
                     /gene="zwf"
                     /locus_tag="PKW26_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOQ92684.1"
                     /translation="MSGEKMSASFVIFGATGDLTHRKLMTAFYSMIRDGLLSDDFCIV
                     AIGRRKLSTETYREQVCASLGKFLKSPVDTKIWQRLERMIFYYEFDFTQSDGYRDLNS
                     YLAKRDKESNTKENRVFYLAVAPSFFETIIHGIERGELASSCRGFCRCVIEKPFGKDL
                     ETAKKLNDTILRVFPEKSIYRIDHYLGKDMIQNIMILRFSNLIFESLWNNRYIDNVQI
                     SLTEKLGVETRGDYYEDSGAMRDMVQNHILQIVSLVAMEPPVNLKMDSIRNEKQKVLE
                     ALEKIDETFLENNVVFGQYAEGFIDGKKVQGYRSEEKVDAKSETETFVALKLQIENFR
                     WAGTPFYIRTGKRLKEASGEIIIQFKELPHSLYNAEKNLAKPNLLILRIQPKVGLYFQ
                     FNTKDYASHSGIIPITMDTGASDAAQGNTPEAYERLLYDILRGDGSLFSRWDELEAAW
                     LFADKLIEYREQKKTDFPNYAAGTMGPKRAFDLIEKDGRSWVDMH"
     gene            17483..18184
                     /locus_tag="PKW26_03595"
     CDS             17483..18184
                     /locus_tag="PKW26_03595"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOQ92685.1"
                     /translation="MICFNPKEIIDISMPLVEGMKVYKGREAKKPRFENQVNHQTGTV
                     HESLLSMNLHTGTHIDTKLHMIDDGEKLEALAISDFIAEAIVLDFSHIQNAITKIDIE
                     EKIASLVKKNILKKGSVTTILKEHFVILKTKNSFEDILEGAFIYLQEDGAEYLAKMGL
                     RGVGIDALGIERDQPNHGSHLALMEEDIHILEGLRLGHVAEGCYTLLALPLSIIGVEA
                     SPVRAVLFPSTEITR"
     gene            18190..19479
                     /gene="aroA"
                     /locus_tag="PKW26_03600"
     CDS             18190..19479
                     /gene="aroA"
                     /locus_tag="PKW26_03600"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOQ92686.1"
                     /translation="MEYKEIKKLSVSSAVLNPPPSKAHTLRALILASLCKGSSTIKNA
                     LLADDQKYLIDCLRALGVSISIERGDEGENSIHVEGKGIPFHVENTKLDCGESGVAMN
                     FLSSLACFANETLILSGKEGLLKRPIDDLTSALESLGVAISFLDKDAYPPLKIKPGLF
                     NKTEVSLSGKKTSQYFSSLSIAGALNPEGLIIHCTDDLSEKPYLDITLEMMKAFCVLA
                     VHEDYKTIRVFPKQEFRPQKIRIEGDYSSASYFFLVAAITGIEVKMPYLKPDSVQGDK
                     KIIDFMQEMGCSIWEEGDALVVKGGNLKAVDIDCLNTPDIVPTLAVAAAFAEGESILR
                     NISQLRYKESDRISAIQSELGKMGIKTALKGEDLCIFGSPKEIHSAIIDSHNDHRIAM
                     SFAVPGLILEGQKIMNPSCVAKSFPHFWELFDGIFTQ"
     gene            19460..19822
                     /locus_tag="PKW26_03605"
     CDS             19460..19822
                     /locus_tag="PKW26_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92687.1"
                     /translation="MEFLHNNAAENARLQKRKNSLAGTLAKTLGFVSISFIILLLFLA
                     LNSFTVQGYARLINESGKIRGGIQRVLQLDPESQEFRDIANQIDASIYELQKLEKSCP
                     LLYGTYTSQKRYCRSLWR"
     gene            19773..>20051
                     /locus_tag="PKW26_03610"
     CDS             19773..>20051
                     /locus_tag="PKW26_03610"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOQ92688.1"
                     /translation="MVHTLRKNDIAVRYGGEEFLLLLRDINSTACFHTCERLRHCVED
                     ESKKTGVPITVSIGYAMFTKNLSSVQHISNVDKALYASKEKGRNQTTNF"
BASE COUNT         6604 a         3145 c         3825 g         6277 t
ORIGIN      
        1 tccacatgtg ttgtatgagg gaacatatcg attgcttgga gttttgtgag gctgtagttt
       61 tcagagcttt tttctcggag gcgttttatg tcgcgagaga agcttgctgg gttgcatgaa
      121 acgtaaatta tttttttggg attttttttg ataatttctt ttatgagttt ttcttctaag
      181 cctgctcggg gaggatcgac gatgagtaaa tcgatgggtt cagaaatttt ttctaaagca
      241 tgttctgctc gtgagtgtat aaattgtgca tttggtattg agtttcgttt tgcatttgct
      301 ttggcgtttt cgactgcttc ttttactgat tcaaccccaa ttattttttg tgcatgagaa
      361 gcgagataga gtcctattgt tccaatacca caatagagat cgacgacggt ttcgtttcca
      421 gaaagggatg catattgttt tacgattttg taaagctgtt cagtttgaat atgattggtt
      481 tgaaaaaaag attgtggtga aatttcaaac gaaagacttc caatagaatc aattaaaaag
      541 gcatctccaa aaagctgtat gaaatctttt ccattactat gagatgcgtt tggtgaatat
      601 gaaagataga gtgaagtcaa ttctggaatt gctttttgaa gtgcagaaca tttttctgta
      661 aaagattttg cagtaaaaga ttcatcggta aaagattgtt taatagaaga gtttgtattc
      721 gtttttatcg tattgaaaaa tctgagatca tcagttacta aaataagcat gaggctatta
      781 ttttttgaaa tgcgtgtaac aagatgacaa agctttggaa ataaatcttt taatacaagg
      841 cgagcagtat ttgacaaagt gttttgaatt aaacaatttt cagtcgaaat tatctcatga
      901 gtttttttct tgtaaaatcc tgcatgaagg ttttgagaat cggtttttga atcggctgtt
      961 agattgaatg ttgaatcaaa tattgagccg ataggaaact cagctttatt tctatactgc
     1021 caaggatttt ccatgccaat tatattttct atttttatat tgttttcaat taggttaaaa
     1081 ttgccaaggc gtttgaggtt ttgaagaaca aggttttgtt taaatgcaag ttgtgcatca
     1141 taagaaagat gttgtaaaga gcagcctcca caaagaccga aaatcgggca ttgtggtttt
     1201 attcgttttg ttgattttgt attaattgaa attaattcac caagaaaaaa gtttttttta
     1261 tcagcgatta ttttgcaatc aataatttca tcgggtaggg ctccatagtg ttcatcttgc
     1321 aaaagctggt tttcggtatt ggattttgaa aatgtgttta ccttcatagg ttcaataaaa
     1381 attgtttttc cattgatttt tgcaactgca tgtgcttctg agtttaaatc aattgtttta
     1441 atattaggtc ccgtgcctat gctttcattt tttttcattt taggataaag tatactatta
     1501 aatgtaattt atttctatga gtgaattgaa atcgtgttgg aggaaaacgt ggactttata
     1561 caactaactc aagtgagtga aactttattg acgctttgta ttatgctttt tgcaggtttt
     1621 cttttaactc gactcacaaa gatggcaaaa ttacccaatg tgacgggcta tattttggct
     1681 ggtgtgttga ttgggccgag cgtattgaat ataattccaa aacaaattgt agccaatatg
     1741 agctttgtaa gtgatattgc gcttactttt attgcattca gtgtggggcg gtttttcaaa
     1801 aaagaaaagc tcaaaaaaac tggtggcgaa gtattagtca ttacgctttt tgaatcttta
     1861 attgcgggga ttgttgttac ttttgtaatg ttttttattt ttaagctaag ttttgcgttt
     1921 tcacttttgc ttggtgcgat tgctactgca actgctcctg caagtacgat tgttacaatt
     1981 cgccaatatg gtgcgagagg aaaatttgta aatacgcttt tgcaagttgt tgccctcgat
     2041 gatgcaattt gtcttattat ttttagtatt gcaactgctc ttgttggcca tgaagctggg
     2101 caagctgctt ctctgcaatc gattttatta actcttttat ttaatatttt atcagttggt
     2161 cttggttttc tttgtggatt ttttttaagt cgcttaatga ctccaactcg tagcgaagac
     2221 aatcgcctta tcttaacgct ggctctcttg cttgggattg cagggctttg tgcacttgtt
     2281 catgtttctc cattgcttgc ctgcatggct tttggaatgg catatattaa tttaacgaaa
     2341 gacaaagttt tgtacgaaca agttgataga tttgcgccac ctattttatc attgtttttt
     2401 attatttctg gaatgaattt agatcttgcg tcttttgcaa gttatgggtt aattggtgtt
     2461 gtttatttta ttgtgcgcat tatcggaaaa tattctggag catggctcgg ctgtgcagtt
     2521 acaaaaacct ctcataaaat tcgtaactac ctaggctttg ctcttattcc acaagcaggt
     2581 gttgcaatcg ggcttgcatt tttgggacaa aggcttttgc ccgaagattc tggaatcatg
     2641 ctcttgtcga taattctttc atcttcggtt ttgtatgagc ttgtcggacc tgcaagtgtc
     2701 aagtttgctt tgcatcgttc aggtgctatt gaaaaagggc aaaaaaagaa aagagaaaaa
     2761 gaagaaaaag ctaaggagaa aaaataaatt gaatatatgg cgaaatcgat gtgaggtcag
     2821 ttgaaaaaaa tactacaact ttttgaagct ttctcttact ttacagctgc tcttttctag
     2881 gatactatgt ttattaagtg agattgtatg aaaaatagtt cgcttgagta ttttacacat
     2941 tttcttaaaa ctgactctgg agcaaatctt cttcgagcaa agtctgctgc ctttgtaata
     3001 acttttttat ataatgaatt tcgtgtagat catgttcctt ttattttagc tgatgatttt
     3061 gaatctcatt tggtttcttt tttgcaaaat aatgttattg atgtggaaac cttactcatc
     3121 tcagaagaag aagcgcctca agaagttttg cttggtgata ttcaagcaaa agcaaagttt
     3181 ctcgtgagtt attggtgttc agaaaataaa aattatatta agagatatta ttcgcataat
     3241 aaaaacttcg tgattgaatt gcatccaagc attgaacggc tttttacttg gctagagtct
     3301 tgtgagccag aagaatttgt gggaacggaa tcgcgatttc aagatatttt atttcaattg
     3361 cgcaatctta aaacaaatat ggatcaaaat cctgagttaa ggatagcgga attaaaaaaa
     3421 gagaaaaaga gaatagatga tgaaatccag cggatcaaag aatcagggcg tgctgaaact
     3481 ttcactccag tacaaattca agaacgcctt ttaaatattt ctcgttcatc gcgtgagctt
     3541 ttgggagatt ttcgtcaagt tggagaaaat tttaaacaaa ttctcaatga tatttatcgt
     3601 gaacaaactg aaacgaatgc tacaaagggt gaaattttag gttatacact cgatacaaat
     3661 aggcaactaa aattatctcc acaaggacag tctttttcaa ctttttggaa atttatttca
     3721 caagatagtg ataatgaaat aaatacttta atagaaagta ttattatttc tcaagctgat
     3781 agaggtttag tatattcaga tgtttttttg agtcgtttaa aacattactt atatcaagaa
     3841 gggcataaaa taattgaaca aaatcgacag ctcactgata aaataaatcg agttttatcg
     3901 cgtcaagaac aagatgaata tcagcgtgtt aaagaattat caaatgaaat aaaatcactc
     3961 gtaaaagctt tgttttcaaa agaaaaaaaa ttgccagaac aatgtgctat ggaaatcgaa
     4021 ggaaaagcag aaatctatgc accacaagca cggtatcctg tgttaaaatc agaagaaaca
     4081 atttttacag caatggaaag ctttgatagc aggcaaataa atcaagaagc actagaagat
     4141 gtgtttaatc aattttatat tgatgaaaaa ttactgttat cttatgcaga tgagtttcgt
     4201 aaaaaaatga atggaaaaca atttacactt gcagaaatgc ttatatctta tccgattgaa
     4261 aaagggcttg ctgaaattat tacttggttt tccatcgcag aaaatgatac acgtattatt
     4321 atacaaaaag aaaaaaatga tagtatcgaa tataataaaa acggagactt aataaaagtt
     4381 aagcttccaa ggatggtttt ttcatgagtc aaaaagaatc ttcgcctcca tgggcaccag
     4441 tttgcattaa acttttgcaa ggtccattat atagagctaa tgcaaaagat atatattggg
     4501 acttactcat ctctcatgaa tctcatattc agtcgtattt tgcaacgctc ggtgtatctg
     4561 tattagttga taaaagtgat ggctatgctt ttctttcaca aatagatgat gaacaaatag
     4621 attcaaattc tttgccaaaa cttattcgaa gaataccttt aactccagaa atttctttat
     4681 tgtgtgtgct tcttcgcgaa gctcttgatt cttttgatag tgcccaaagt gattcttctg
     4741 ttcttgtaat gacagctcat gaaataaaag acttgctagc tttgtatatt aaagaacaat
     4801 ctgatcaaac aaaatttata acaaaactag attcatatct tacacaactt gttggattta
     4861 cttttttaaa agaattggct ccagaaaatg gaggagtcct ttcaggttta gaaagaattt
     4921 ttgaagtgag aagaattatt agagcaaaaa ttgatgcaga atttttggaa gaatttaaaa
     4981 gaaaactatc agaagaagct gaaaaaattg aaacagaaca ggaggaagtt taatggcgaa
     5041 cgaacttttt tcatctggag gttatcgtct tgatgttttt caactttata actggggact
     5101 ttttgacaaa gtaatttatt ctctcgattg tgctcaagga acaactttat taacgggggc
     5161 gaatggcagt ggtaaaacaa ctatagttga tgggctttta tctttgcttg ttcctccaca
     5221 acaacgacat tataannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngattg
     5341 agcaaataaa caaaattctt tcagaagaag aaagtagttt tgataaacaa ggaagatgga
     5401 aacgaatttt aagtgaaaaa tataatacgg ttttctttgg agatagtttt aaaaaatatt
     5461 ctgattttta tagcagcttg tttggtttta aatcagataa agctcttagg cttttttctc
     5521 agattgttgg tctgaaagtt cttggaaatt taacagaatt tattcgcaca aatatgcttg
     5581 aagaaactga tgtatatgct gaatttgaaa aacttgatac gaattatata aaattaatgc
     5641 aaagcaataa tgaaattcaa aaaacaaaaa aaattcttga gcttttagag cctatacttg
     5701 aattaggtaa aaaacttgaa aaggaaaaga gtgagaaaaa tcgtttagaa accatttcta
     5761 gccttgttcc actttggaaa gcggaaaaag caattttttt actaacagaa aataaaataa
     5821 ttttggaaag aaataaagca gatctcgaat ctcagctttc acaaaatagg aaagaaaaaa
     5881 gtgaaacaga taataaaatt tcaagcttgc aaatttctct tgcaacgaat gatacacaaa
     5941 gtcgtataga aactattgat agagaaataa atattaaaac agatgagaaa aaacgaaggg
     6001 atttaaaacg aagtcaatat gaaaaaatgg caaacgaagt aggaatttca attcctacaa
     6061 atgaaacaat gtttagaaac aattttgaaa aaatagtaga aaaggaaaaa gcagaaattg
     6121 aactaaaaaa aactaatgaa gatatacgtt ttaataatca gacagaattt aatactcttc
     6181 aaaatgaagc agaaataatc aaaaatgaac ttgcttcttt aagcaaaaga aataccaata
     6241 taccagatgc taatattaaa attcgagata gcatttgtca aaaaattaat tgtaaagctg
     6301 atgaaatacc ttttgcaggt gaacttattc aagttgcttc taatgaacaa aactggaatt
     6361 atgcaataga aaaacttctc cacagttttg ctctcacact tttagtccct gaaaaattgt
     6421 ataaaaaagt taccaattat gttaaagaaa ataatatgaa agggagagtg gtttatttta
     6481 aaatagaaga agatgttttt tctcttgaat caaaaataca attacaaaaa gattcagata
     6541 cagtaccagg aaaacttgaa attaaagaaa atgtatgggt gagagattgg ctggtagatt
     6601 atatagcaaa acaatttgat tatcgctgta ctgataatat cgaagaattt tctcgtgctc
     6661 ataaagcagt atcatcgaca ggtttaatta aaaataaaat gcgccatgaa aaagatgacc
     6721 gacaatttag tgatgagaaa aaattgaaag tgcttggttg ggataataag gaaaagaaaa
     6781 atattttaag ctctcgttat gatgaaattg aatctttgca attaaagcaa acaactttac
     6841 ttaatgaaat agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntattgtat
     6961 gaaaagctaa aaaaagatga tttaccaaaa tatcaagaac aatttcatga atatttacaa
     7021 gatacaatga ataatgatat tgttgatttt aatcagttta ttcaaaatga agaacaagaa
     7081 ataagaacag ctatttcaga tttaaatgca agtttgcgaa aaataagata tgagcataat
     7141 ccagatacct atttacaatt agaattttca aaaaataatg atgtacggat aaacgagttc
     7201 aaagagcatt tgaaaaatgc aatcccaaat gcctttgatc tttcttcttt tgattctgaa
     7261 aaagaagaag aactatttaa taaaataaaa ctttttttag atactgtaaa agcaaatgat
     7321 gcaaatcgaa aatatatact tgatattcga aactggtata catttgcagc atctgaaaaa
     7381 tataaagaaa ctggaataca aaaacaattt taccctgatt caggtagttt aagtggtggt
     7441 gaaaaagcga agcttaccta tactatttta gcatcagcga ttgcttatca atttgggctt
     7501 acacaagaag aaagtgcacg ttcatttcgc tttgcaatta ttgatgaagc ttttagtaaa
     7561 agtgattctg ctaactctga ttatgcaatg cgtttattta aacaactaga tttacaaatt
     7621 atggttatta caccgcaaga taaaatcaat attgtagaag attatatttc tacagttcat
     7681 attacagaaa aaataggaac caaagattct cgtttattac ctctgactat tgaacgttat
     7741 aaagaacaac aaaaagaagt tttacaaggg aaaaaaatat gactatgatt actgtaaaag
     7801 aaataaaaca aaaagctttg aatagataca gctctttttt aaaatataaa attgttttgg
     7861 aattggaaag cgaagatact aaaattcaaa atgctttagg tgaaaatggt tttgattcga
     7921 aaagagattc tttttttcct ttagaaatac aagggaataa aggtaatcca aatgatgatt
     7981 ttaaaaaaag aagtgcagca cttcaagaat tgattaaagc aagcaactta aaaaaactct
     8041 ttagttatgc tcttgaaaca aaggatgtct taactcgtaa taatggaact caaactatta
     8101 tacaaaaaat atatttcgaa aatgaaaaaa gctttctggt ttttattgaa aaagaattgg
     8161 aagtaaaaag gttttgtgaa gcattaaaaa tcataaataa tgcaaaattg aaaaactttg
     8221 aaatgcaaac atgggcagaa aaaaatataa aagccttgtg tactgaaaca gaagaacatt
     8281 tttggcaaaa tattgtttta gttattgctt ggttaaaaga taatccaaaa agcgatttgt
     8341 atattcgtga aataccactt ccaatacata caaagtttat tgaaaataac aaaaagctca
     8401 tagaaagttt tttttatact gctaacaatc agtcaaataa aaaaccgtcc tttgaaaaac
     8461 tacttggact taaaagtaat cctttgttgt tttattttcg ttttttagat ccttcttttt
     8521 cttttgttga agggatgcac tctcctcaac tcatggctat ttctctttct cattttaatg
     8581 attttttgga aacagaaaaa ctgaaacaaa tacagacagt ttttattact gaaaataaaa
     8641 tggtatgttt agcttttcca aaagttaaaa atgctctttg tcttttggga catggttatt
     8701 atttacttaa cttaaaaaac tgttctgcat tgcaaaataa acaaatattt tatttaggtg
     8761 atattgatga acatgggttt gatatacttt caaaatttag aaattatttt ccacatacta
     8821 tttctctttg tatggataaa aaaacctatg aaacatttaa gttttttgca gttagctcag
     8881 ataagaagct tccagataac tcgattccaa aaaacttaag agatgatgaa cttgaaattt
     8941 tcaatcactt acgaaaagaa cctgaacata atcgccttga acaagagaga attacacaag
     9001 actatattat ttcacaatta aaaaagaaaa atattttatt atgattcttt tttctgcatc
     9061 atttcattat acactttttc acttatggga taaaagataa gtgtaataat ggcagctaaa
     9121 aaaataagtg cagggatggg gccaacgagg aggcgaattg taaaaattgt tttattattt
     9181 tgctcaatat ttggaagata cccgctcatg gttaataaga gccctaaaag tccgattgaa
     9241 agagcttgtc ctacttttga aaaaaaagtc cacatgccat aataaatacc ttcttttcga
     9301 actcctgttt tgatcgcgtc ccattcgact gtgtctggaa ccattgccca aggagttgca
     9361 aacactattc ccaaacctat tccaacaaaa gccattataa taaaaacaaa aacaaagcca
     9421 aaaatatgac caaagaaaaa tatcgccatg caacctagag ccataagtcc aagcccgact
     9481 tgataggtga gtttttttcc aagtttttta gaaagcgaca ctgaaggtcc gatgcttatc
     9541 attgctataa ccaacaatac aatgagtgca atttgtgttt gctcttttgc gattaagaca
     9601 tattcaaaat aatacactaa gattccttgg agtagattga ttgcaagaat atgtaatgca
     9661 aaaacaccaa ctaaaaaaag atatgctttg tttttaaaaa cagcagaata ggttttgaaa
     9721 aatccttctt ttggaataga ctccaatgca ggcggatttt cttttacact gaaagtagta
     9781 atgagcgcag tgataagcat tacaagcccc ataataaaac ctgctgcgga aaagcccttt
     9841 gattcagtag agaataatcc gaggatcgga agtacaattg cagctccgat aattgtaccg
     9901 atgatagcaa aagtaaaacg gtatccgtta agagcacttt gctcatggta gtctttagtt
     9961 aattcaggag ttaatgaaga gtagggcaca ttgactatag taaaagccgt atttaaaaaa
    10021 caaagagcta aaactgccca gactgtaagg agcgtttgag attcgaggtg tggattggta
    10081 aagaaaaacc acattgataa aaaaagagga attgagccat agagaatgta ggggcgtctt
    10141 ctccccattt tgtgttttgt gcgatcagaa atatagccca ttgctggatc agttactgca
    10201 tcccaaaact ttccaatcat aatagcaaag cctgcagctg ctgggagtag ggagacgaca
    10261 tcagttaaat agtagagaga ccaaaatccc atcgcagtaa aaaataaatt gccgcccata
    10321 tcacagacgc caaaaccaag ctttgtttta agactcaatt ttttctcttc cataataaaa
    10381 actcctttaa tgtccgtgtc tgttggtctc ttagagaccc attattgctt gtgaaatact
    10441 aaagaaaaag ataattccga gtatatcagc catagaggtg atgagggggg agcttgcggt
    10501 tgcagggtcg agcttaaaga cgcgcaaaac aaaggggagg attgtgccca aaagacttcc
    10561 ggttatgacg actgctgcca tggagagagc aacgcttagc cccattaaaa tacctccacg
    10621 gtaaatacct aaaaaagaaa ccgcaaagcc catggaaata ccgagcatca ttgaaacgag
    10681 gagatctttt agaacgactt cccaccattg tttgagatgc acttctcctg ttgcaaaggc
    10741 acgaataatc attgcagatg attgtgagcc tgcatttcca ccgctgtcta ttattaaagg
    10801 aataaaaaaa acaagggcgg ttactgcttg tatgagctct tcaaagtgag ccatagcaaa
    10861 accagatata acatttacga agatgagggc gataagccag ccgacgcgtt ttttgaaaaa
    10921 atctaaaacc tttgtttgca tatagggtgt ttcgagagga gagacagagg ctgttttgtg
    10981 gaagtcctca gttgcttctt ctttggcgat ttcaagaata tcatcgaagg tgatgcttcc
    11041 aagtaaaaca ccttcagaat caacgacggg taatatgggc aagccgtagt gttcgagata
    11101 atataccgct ttttcttgat cttcaaaggc tgaaagactg acataggtat tgtccatgat
    11161 gctttcaacg agagcatcat catcgcttaa aataagactt cgcaattcaa ttgcatcgag
    11221 cagtttccat tgattatcga taacataaac aatgtcaaag ttttcaacat ttttacctac
    11281 ttttcgaata tggtccattg cgcgctgaat tgtccagttt tttttaatag cgacataatt
    11341 cggtgtcatc aagcgcccta cggaatattc aggataaccg agcagttcaa gcgttttttt
    11401 tcgttcatct acgggtaaaa attctataag tttgcgtgta acttctgagg ggcattcttc
    11461 aaaaaaccgg gttcgatcgt ctggttcgag ttctgttagg atttggcgaa tttgtgtgtt
    11521 attcattcca gaaagaatgt cttcctggag atcccaatcc atttcagaaa aaacatctgc
    11581 agcaaggtct cgaggaagga ggcgaaaaat aatgacgcga tcttctgtgc ttgaaaccga
    11641 acgtatcaaa tcagaggtgt ctaccggttc taaaccaggt aaaacttctt taagactctt
    11701 ccaatcgtgt gaattgatta attcttgaat ttctggtgta atgacattta ttaacatcga
    11761 ctttaccccc cttattgata tgttatttat tgtatataat agtaccatta tagtataata
    11821 aaccaatttt gcatactata taaagtatta ttattggaaa agagcaaact ctatagaata
    11881 gcgaagctat gtaaatcgca aaggatttta agtaaaaaat gatagaaaaa gccttgttag
    11941 aatatctaca gcctgtttta caaaaaaata cgcagctttt ttctcttagc tgtcctgaat
    12001 ggattttgtc aaaaaagcgt ttcctattgg ggaaagagta tgttttttct ataatgcttg
    12061 ttttacatac agatctcatc ccgatggaaa tactttttca agaactcgac agtcttttta
    12121 aagagattgg cttagatcct aaactcaaaa aaaaaataca gtggtttgtg ctctctcccc
    12181 gtggaggaat ttacctttat gaaagtatcg caatacggga gcacaaaaaa aataaaaaaa
    12241 tgcgcaatcg gtttataatt atcaatacgg aaaatgcctc tgtctctttt ggaccgggac
    12301 ataaaaaaaa tactactgca tctcaaaagc tttttagcct catacaaaat agtcgttttt
    12361 ttagaagaga agaatggctt tcgaaaatgt ttgacttttt ttcaatttgc gaagatacta
    12421 tcaataaatg aaagaagcaa acagaccgaa cctttataag ataagtttaa agttcttcct
    12481 cttgaaataa ttctcttgta ttttctttgt ttactcttat tatgtggaac aagtatttat
    12541 tttatcttgc ataattcaaa acttgcagat aaaacctatg gtgagcttgt ttcaaaacat
    12601 attttataga tataaaaaag ataatttaga atatacagtt gaaaatgcaa tcaaaagctt
    12661 tgaacaaatg aggaaaaatg ttgaaaagca gcttgatgag gaacaagcat tttttttaga
    12721 aaagatttct gaattagtta cttattatgg caaagatgaa gcaggcattg agttgagatt
    12781 gccaaacatt cttgcacaaa ggctcattga agcagtaagt aatacagcaa taataattac
    12841 gcatccaaaa agaaaaaaaa taaaattttt acaacaaaga aaaaaagatt cagaagatta
    12901 tactattgat tttagaaaca atgagtattc tagtgaaacg acaacaagct tgttcctaac
    12961 tatttctgcg ggaatttctt gtttttctcc taaagatacc agctaccttg aatgttttaa
    13021 aaggactgat gcggccttgt atgagtcaaa gaataccggc aaaaataaat ataccatatt
    13081 gtattagttg gggtggagat ttttttgttt tacaggctgc aaaaaagagg ctttgttatt
    13141 tttttaacaa gcttgcaaag gggttgtaag catcgtcaac attttgatgt tttttaattt
    13201 cctttgcgtt ttcaatttct tcttttgttt caccttgttt gagcgctatt tttttattgt
    13261 agctatcgac cgattttata ataacttcga ttgtatcgcc tttttttaag tttatatctc
    13321 tattgcctct ttcgtcttta cgtatttctg aattatgtaa gagcccttca agaccttctt
    13381 ttagtcgcac aaaaacacca aaacctgcga ttcgtgttat cgtaccagtg tgaattgact
    13441 ctggtgcaaa gtcttcgagg ttttcccatg ggtctcccag gagagctttt ttactgacgc
    13501 ttattttgtc attttcccaa tcaagattga gaattgcaac ttcgattgtt tccccaaggc
    13561 tgagaaagtc ttcgatttta tcaattcgat cgaagcgtat ttcgctaatc ggtagaagtg
    13621 cttttatgcc ttttaaatcg acaaaggctc caaagggatg gagggatttc acttcgacgg
    13681 acactttttg tccgacggat agtgttttag aaagttcttt gagggcgttt tttctttcct
    13741 cttctaaaac agcaagatgc gagagagtga ggcttctgcc accatctttg tatgtgataa
    13801 ttttaaaagt gagtactttt cctatgatat tttcttgctc ttcttttgct atgccaagtt
    13861 gagaaaaggg acaaaaggct gcgtgttttc cgatgcttac ttcaaagcca ccttttatct
    13921 ctttttctat tttgccttcg acaggaatag aagcacggta ggcattttca agcatttgta
    13981 gagaagcctc ttcttgagca atttttgtgc taaaaaaaag ttctccgttt tttgcagcta
    14041 taaaatgtgc ttctacaaag tcgccttctt ttatgcgtaa attttcctca tcgtcgagaa
    14101 attctttttt ttctattatg ccttcgcttt ttccattaag gtgaataaaa acgtattctt
    14161 tagaaaaaga aacgacctcg gcttttattt tttgaccagg ttctagtctt ttttgattgg
    14221 gaagggattg ttctaaaaga gcttcaaagc tcgcattatc gctcgtattg tttgtttcgt
    14281 tcatacaatg agagtacgag aaaatagaat ttaggtcaag caagcaaaag gctcgttttt
    14341 atttcctact agtagacttt gtttaacttg ttttttttta gactatcgat tagagtataa
    14401 gaatattttt tacatccttg taaaaaatat tcggacagtt ttgctttgca aaacacgtca
    14461 aagattggtt gcctctcgcc atccgtggcg agaaagtatg tttttggaat gaaggataag
    14521 gtatgggaca aaaatacagc acaatagggc tcatcggtct tgcggtgatg ggcaaaaatc
    14581 tcgctttcaa tatggcggat aagggttttt ctgtttccgt ttacaataga agttttgata
    14641 aaacaaagga tgtttttgca gaatatacat cggaatcttt tcgaggtttt gaaaaactcg
    14701 aagactttgt tctctctcta gaatctcctc ggaaaattat gctcatggta aaggcgggag
    14761 aggctgtaga tcaaacgatt gcgcttttgc ttccccttct ttctcctgga gacctcatca
    14821 tcgatggagg aaattctcac tttaaagata cgcaaagaag aacaaaggct cttgaagaaa
    14881 aaggtctttt gtttttgggg acgggagttt ctggaggaga agagggagcg cgctttgggc
    14941 ctgcgattat ggtagggggg agtgagacag cctatagcct tgttgaaaat attttttctg
    15001 ctatttctgc aaaatacata ggacaagatg gcaagagcga agattgttgt ggctaccttg
    15061 gaagtgatgg tgcagggcat tttgtaaaaa tggttcacaa cggcatagaa tatgctgata
    15121 tgcaactcat tgctgaagcc tatagtcttt taaaagagga tttttcaaac gaagcgcttt
    15181 ctcagatttt tgccgaatgg aatgaaggcg agctggaaag ctatttaata gatataacaa
    15241 aagatatttt taagaaaaaa gatgcagaga cgggagccca cctcgttgac ctcattctcg
    15301 atagggctgg tcaaaaaggt acgggcaagt ggacggctca aatttctctt gatctcgcca
    15361 gtccaattca aagtattaca acagcggttt atgagcgttt tatgtcggca agaaaggatg
    15421 agcgtatacg tgcagcttct ttgtacaagg ggcaggcaga aaatggatgc gagtctaatt
    15481 ttgcggctag tcaaaaaagc gatgcagaaa aaaaagctct tgttgaagaa gtgcgtcgcg
    15541 ccttgtacgc gagtaaaata tgcgcctatg cacaaggctt tagcatgctc ctcgatgctt
    15601 ctcgggaaga aaactggaat cttgactttg cacaaatcgc aaaaattttt agaggtggtt
    15661 gtattattcg agcgcgattt ttaactgagc ttgcaaaaga gtttgatgca aaagctgact
    15721 tggaaaatat tcttctctct cccttattta gtgcgacgat taaaacatac gaaaaagatt
    15781 ggcggtcagt tgtatcaaag gcaattctcc aaggaaggtc gcttccagcc tttagttctg
    15841 ctctgagtta ttttgatagc tatacgacaa aaaatcttcc gacaaatctc ctgcaagcga
    15901 tgagggatta ttttggcgca catagctatg agcgcataga caaggaaggt tttttccaca
    15961 cgaactgggg ggaataatga gcggtgaaaa aatgtcagca agctttgtta tcttcggtgc
    16021 aacgggagac ctcacacatc gaaaactcat gaccgctttt tatagcatga ttcgagatgg
    16081 cttgctttca gacgattttt gtattgttgc aattggtcga cggaagctct ccacagaaac
    16141 atatcgcgaa caagtctgtg cttctttggg aaaatttttg aaaagccctg tagatacaaa
    16201 aatatggcag cgcttggaaa gaatgatttt ttactacgag tttgatttta cacaaagcga
    16261 tggctataga gatttaaact cctatcttgc aaaacgcgac aaagagtcaa atacaaaaga
    16321 aaaccgagtt ttttatcttg cagttgctcc ctcctttttt gaaacaatta ttcatggcat
    16381 agaaagggga gaacttgcat cttcttgccg aggcttttgt cgctgcgtta ttgaaaaacc
    16441 ttttggaaaa gatttagaga cagcaaaaaa actcaacgat actattctcc gtgtttttcc
    16501 tgaaaagtct atttatcgca ttgaccatta tttgggaaag gacatgatac aaaatatcat
    16561 gatactgcgt tttagtaacc tcatctttga atcgctttgg aataaccggt atatcgataa
    16621 cgtgcaaata tctcttacgg aaaaacttgg ggtagaaacc cgaggggact attacgaaga
    16681 ctctggtgct atgcgcgata tggtgcaaaa tcatattttg caaattgtca gcttggttgc
    16741 aatggagcca ccagtaaact taaagatgga ctcaatacga aatgaaaaac aaaaagtctt
    16801 agaggcattg gaaaaaattg acgagacatt tttggaaaat aatgttgttt ttggtcagta
    16861 tgctgaaggc tttatagatg gaaaaaaagt gcagggctat cgctcggaag aaaaggtaga
    16921 tgccaaatct gaaacagaaa cctttgttgc attgaaacta caaatagaaa attttcgctg
    16981 ggcaggaacg cctttttata ttcgcacagg aaaacgtctg aaggaagctt ctggtgaaat
    17041 tattatacaa tttaaagaac tgccgcatag cttgtacaat gcggaaaaaa atcttgcaaa
    17101 accaaatctg cttattttac gcattcaacc aaaggtagga ttgtactttc agtttaatac
    17161 aaaggattat gcaagccatt cgggtattat tcccattacg atggacaccg gtgcaagcga
    17221 tgcggctcag ggaaatacgc ccgaagccta tgagcgtttg ctctacgata ttttgcgggg
    17281 agatggctct ctcttttctc gttgggatga acttgaagca gcctggcttt ttgcagataa
    17341 acttatagaa tatcgcgagc aaaaaaagac agactttccc aactatgcgg ctgggacaat
    17401 gggaccaaag cgcgcctttg atttaattga aaaagatggt cgctcttggg tagatatgca
    17461 ctaggcgaaa aggaggatgc atatgatttg ttttaatcca aaagaaatta ttgatatttc
    17521 gatgcccctt gttgagggga tgaaggtgta taagggacgg gaggcaaaaa aacctcgctt
    17581 tgaaaatcag gtaaatcatc aaacagggac tgtgcatgaa agtttgcttt cgatgaattt
    17641 acacacgggc actcatatcg atacaaagtt gcatatgata gatgatggag aaaaattgga
    17701 agcgcttgca atttctgatt ttattgcgga agctattgtt ttggattttt cacatattca
    17761 aaatgcaata actaaaatcg acatagaaga aaaaatagca agccttgtga aaaaaaatat
    17821 attaaaaaaa ggctcggtta caactatttt gaaagagcac tttgttattt taaaaacaaa
    17881 aaattccttt gaagatattc ttgaaggagc ttttatttac ttacaagagg atggtgcaga
    17941 atatttggca aagatgggct tgcgaggagt tggcatcgat gcactgggga tagagcgaga
    18001 ccagccaaat catggaagcc accttgcgct catggaagag gatattcata ttcttgaagg
    18061 cttgcgattg ggacatgttg cagaaggctg ctacaccttg cttgcattgc ccttgtcgat
    18121 tattggtgtt gaggcaagcc ctgttcgcgc ggttttattt ccaagcacgg aaatcacaag
    18181 gtgaaaaaaa tggaatacaa ggaaataaaa aaactttctg ttagctctgc tgtcctcaat
    18241 ccaccgccat caaaggcaca tacgctccgc gctcttattc ttgcttcctt atgcaaggga
    18301 agctcgacca taaaaaatgc cttgcttgct gatgatcaaa aatatctcat cgattgcctt
    18361 agagccttgg gggtctctat ttctatagag cgaggagatg agggagaaaa tagtatacat
    18421 gttgaaggga agggaattcc ttttcacgta gaaaatacaa agcttgactg cggagagtcc
    18481 ggggtcgcaa tgaattttct aagctcccta gcttgctttg caaatgaaac tctaattcta
    18541 agcggtaagg aaggcttgtt aaaaagaccc atagatgatt taacttctgc ccttgaaagc
    18601 ctcggtgtcg ctatttcttt tttagataaa gacgcttatc cgcctttgaa aataaagcca
    18661 ggattgttta ataaaactga agtctccttg agtggaaaaa aaacatctca gtattttagt
    18721 tccttatcga ttgcaggggc tttaaatccc gaaggactta ttattcattg tacagatgat
    18781 ttaagcgaaa aaccctatct tgatattaca cttgaaatga tgaaagcttt ttgcgtgctt
    18841 gctgttcatg aagactataa aacgatacgc gtttttccga agcaagaatt tcgtcctcaa
    18901 aagattcgca tagaaggaga ttattcgagc gcttcgtatt ttttcctcgt agcggctatt
    18961 acggggatag aagtaaaaat gccctactta aaacctgact ctgtgcaagg tgataaaaaa
    19021 atcattgatt ttatgcagga gatgggctgt tcgatatggg aggaaggcga tgcccttgtt
    19081 gtgaagggcg gaaacttaaa agcggtcgat attgattgtc tcaatacacc agatattgtg
    19141 cctacgcttg ctgttgcggc agcctttgcg gaaggggaaa gcatcttgcg aaatattagt
    19201 caattgcgct acaaagaaag cgatagaatt tctgcaatac agagcgagtt agggaaaatg
    19261 ggtataaaaa cagcacttaa aggcgaagat ttatgtattt ttggttcgcc aaaagagata
    19321 cattctgcta taatcgactc acataatgac cacaggatcg caatgagttt tgctgttcct
    19381 ggtctcattt tagaagggca gaagattatg aatccctctt gtgtagcaaa atctttccct
    19441 catttttggg agctttttga tggaattttt acacaataat gcagcagaaa atgctcgcct
    19501 acaaaaaaga aaaaattctc ttgcaggcac ccttgcaaaa accttaggct ttgtttctat
    19561 cagttttatt atacttcttc tttttttagc tctaaattct tttacggtac aaggctatgc
    19621 acgacttatc aatgaaagcg gaaaaattcg cggtggcata caacgtgttt tacagcttga
    19681 cccagaatcc caagaattta gagacattgc aaatcaaata gatgcatcga tttatgaatt
    19741 gcaaaaactt gaaaaaagct gccccttgct gtatggtaca tacacttcgc aaaaacgata
    19801 ttgccgttcg ctatggcggt gaggaatttt tactgctcct tcgcgatata aattctactg
    19861 cttgttttca tacctgcgag cgcctaagac attgtgtaga agatgaatca aaaaaaacag
    19921 gagtacccat aacagtgagc atcggttatg caatgtttac aaaaaacctt agctccgttc
    19981 agcatatttc aaatgtcgac aaggcactct atgcatcaaa agaaaaaggg cgaaaccaaa
    20041 caacaaactt t
//
LOCUS       DAOSSW010000025        19860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_2903_length_19860_cov_7.033931, whole genome shotgun sequence.
ACCESSION   DAOSSW010000025 DAOSSW010000000
VERSION     DAOSSW010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 19860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19860
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2903_length_19860_cov_7.033931"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PKW26_03615"
     CDS             <1..410
                     /locus_tag="PKW26_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HOQ92689.1"
                     /translation="WSNLRNCAYIQEFEKEKIAWNRIASKKLFSLIDEGIIIQDSMHF
                     FTGNHLKFLCAILNSKLFSWFMYLIIGDAAGGNAGNADNVSNLTIIKPTEQLELQIEN
                     LLKNEQYSEIDKIIYQLYDLTDEEIAFIEKASK"
     gene            633..2141
                     /locus_tag="PKW26_03620"
     CDS             633..2141
                     /locus_tag="PKW26_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92690.1"
                     /translation="MDNKKTINTKKTQKSGTKLFYTIGSIVILVLAALAFVLIPALTT
                     TGSRDEIIVGSWNKKPIKYVANSDFTKNVEYYSNYYKQQGYEVNDQNFYTILDHAFTA
                     TVLNRAIIDEVDKAGYIPSQNTINRELLPYFYDTEGNYSAKIFRDTPDSKKIEYKKNI
                     IENLKYGSYVMDHFAQTKVPYYGLKSAKNESVALAKMNAYKKGFDYVAFSTDDYPLSQ
                     ARNFANENPDLFTEYNFSVISFDTEAQAKSVRKQLEQNEITFFDAVENFSTKNYSGDD
                     GIFTYTYQYQIKPLLASEEELKSVLATEVDSLSPIVKTSNTYSIFKANANPKQADFTT
                     DASLDIVLNYLKQYEMGRVQDYFISLANDFSLNASLGNFAGAAEKFNREVKSLEPFAL
                     NYGNSELLYAVPSSSDGLLYGAERNESFLKTAFSLEEGEVSAPIILGSNVLVLQCTEE
                     QNELSESELENYTYIYAYYAGQYNENSFYNYFLSSDKTVNNVFATYLKYFYQ"
     gene            2157..3926
                     /locus_tag="PKW26_03625"
     CDS             2157..3926
                     /locus_tag="PKW26_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOQ92691.1"
                     /translation="MSPDKHSNKRLSSGQPSLEREDADIQKIETKNGFTVLYKNRYLY
                     SRFDAKKNSERLVQSLEIKGESLFFIFSPLLGHGLDLLFEKISSDSFVLAIETDPLLY
                     AFSLQHINKKLLEKKNFLYLCLEEKMTGVKELLEKIDHLVQSEEGYAFRRLVPLELSS
                     TFIQKEFQNTCTQYIDLYINQFWRNRITLVKLGRLYAKNIFQNLALLSQAETIKSATI
                     EKPILVLGAGPSLEENIAIIQAFQKHFFIIAVDTALAYLHKQKIGIDLCLVMEPQLIN
                     QKAFLAVKNTKIPLLADLCSYPATQKILNGPLSFFFSEYASLQFLQNLAKNSGIQKYR
                     ALGSVGLVAVETALKLRKKNTAIFFTGLDFSYQLGKTHTKETPSHLARLFEATRLKPA
                     ENIGLVENKIGDGLYTNPALENYAKLFDDLYAEENELYMLSETSIIRKIKKISLEDFR
                     KWIENKASQTDTSQRDSIEKNEKIESRFFETDTETNTIEKTKRKNAIIDFLENEIKKL
                     SVLSSFLESIKPEQTSSQLDENLQKEVRKKLLGLEYLYIHFPEAYKKPDIHFSFISGL
                     KGEISFFLKIIERSLDRITSEHF"
     gene            complement(3953..5758)
                     /gene="lepA"
                     /locus_tag="PKW26_03630"
     CDS             complement(3953..5758)
                     /gene="lepA"
                     /locus_tag="PKW26_03630"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOQ92692.1"
                     /translation="MTPLVQKRNFCIVAHIDHGKSTLADRLIQKTKVVDDRVFQNQML
                     DNMDIERERGITIKSHAVTIPYTAKDGLLYELNFVDTPGHVDFSYEVSRAIASCEGAL
                     LIVDAAQGVESQTLSNMYLALEQNLEMVPVINKIDLPAADIPRVKKQIDHDLGLDPDL
                     AVCISAKTGEGVDELFEAIVNQIPPPSDDESGFTKALIFDCHYDPYRGVVVHLRIFSG
                     SIKKGDTVRFMVSESDYKVEECGIFRLGLVEKNSLECGDVGYIIAGIKTVSDVKVGDT
                     VTLTKNPVDKPLDGFKDVKPVVYSSIYPVDTSDYEELRESMDKLKLNDASLVFEKDSS
                     MALGHGFRCGFLGLLHLEIVQERLERDFDQSVIFTAPSVRYLITLSNGEEKYIDNPAD
                     FPELNKIAKSEEPYIKAAIITPATYLGNVMALCTEKRGVQTNMQYLDEKRVEIFYEMP
                     LAEVLFDFYDRLKSYTRGYASFDYELIGYRETELVKIDILINGKSVDALAQLTYKGNA
                     VAKARNTCAQLKEEITRQQFKIAVQGAIGSQVIARETVSALRKDVLAKCYGGDITRKK
                     KLLEKQKEGKKRMKMVGDVELPQSAFLAVLKTKEE"
     gene            complement(5771..7504)
                     /locus_tag="PKW26_03635"
     CDS             complement(5771..7504)
                     /locus_tag="PKW26_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOQ92693.1"
                     /translation="MKTKLSTKLFHPFFLILSFFILACQTHSEHHFIPLAYTELDARN
                     GEIDEIRLQIEEKPLESLWRSALLLETLEGFPNEHKDACEAVFDEAKSFVLRETQTAL
                     DEKNIPLAKAFVLSLQNLSVTEIALYTNELERLENDFLKTYFPALGLDENNHALKDSK
                     NSDNAYSEPFPSLPPSKVSQYLQGLVTVWVDRGVSVQRGLGLPDRIIGSGFFIDSMGH
                     MITNYHVIESEVDTTYKGYSRVYVKLYDDVETRYPAKVIGYDKALDLALLKTEVPVPF
                     SFSLGSSEDLEVGDRIFAMGSPLGLENTITSGIVSSKSRSLSTVASAMQIDAAINPGN
                     SGGPIVDQNGLVQAIVFAGVLEYEGLNFAIPVEQLEKILVLLFHGEVKHSYTGFYAKT
                     KRELPQQEALGIEVHYVMRGSAAQKGLLAKGDIVQSLNATKIRTVEDYQNAMLEIMPG
                     TIIKLGGVNQEGEAFTKYLYTEERPEMPIYEAYKREPVEDFFLPSFGMKLKRTSVYNK
                     NQYEIASIIKGSAADENGFSLQDPVEIKKIKFLEKNTIVYAELFTRKRNKAYFEVNLA
                     IGASLDSPYFF"
     gene            complement(7482..9533)
                     /locus_tag="PKW26_03640"
     CDS             complement(7482..9533)
                     /locus_tag="PKW26_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92694.1"
                     /translation="MKKNRAALFLLSLFSFLFLGAQESDYLQQANFQTATRCVELARD
                     QLVAGNYIDAFDFIEVGLFYDESISDLWYLHALLLEYQNKEIFKIIDSVEKALEKKSW
                     LYYTPEAARILYAKYALATGDMHAALKILDEKPVLIQSDAEYLRCLASYRLQDREKAH
                     EIISNARRLFPNDARFPLLFFQNERLMQSNEEAFQALSTSLLAMLEKWKTREPDILVY
                     ASFFVDEDSAVRYLKEYRLTEKKDPLYILAFLEHGLYTEEQAIQALFQIADRGIAAPV
                     FFELLSVLQAPQSFAFFAENLEDFSGRLDFDSNRDGADDFFVYYKYGRPSTIVYDKNA
                     DDALNWIIECDYGLAEKCIDYEKSYEVYFGRYPFVDSFSSFALEEERMEMRFVDASVT
                     WEAYELELIPIFENKVFYLPVLNRDSQAFDLRTMQAFANEIVIYPKENEHKKIRFSLQ
                     NNRIKTALYTLKDKPYAYGFFENGLLQFRNVDRDGNGSYETTEFYAFDPELKSEKNKE
                     ANLYTELFGSFEMPEGYYLSEVRIDTNENGIPDFRESYLPDGRQITAWDENENGSWDL
                     AYEKNMQAGDFLEEKVFFINPLNNEEVEIVLENGIPTIARYADRTKKILKEKDLPFYW
                     LGESSYTRHALKAYTELAKTDIQGMRILIDDHDMRGFRMLAIKIDTFYFGELIEDETI
                     Y"
     gene            complement(9584..10819)
                     /gene="tilS"
                     /locus_tag="PKW26_03645"
     CDS             complement(9584..10819)
                     /gene="tilS"
                     /locus_tag="PKW26_03645"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOQ92695.1"
                     /translation="MSKTKKTSIEEKLIQSLASLPLDATLLLAVSGGADSICMLSLCA
                     RYAKLRNQKIIVASLNHNLRSEKESQKDLDFVKDFCKELGILCLVKTAEPGFIKKLSA
                     KRKKGLEEAARFYRYAFLEDSAKEQGASYILTAHNKNDKLETILERFLQGSLARSGME
                     IMRGNYVKPLLDCSRDEIEDYLEKNNIAYCIDKTNAETVFLRNKIRNRLVVFLNTYFW
                     GWQKALLQGAKKQAIDEDFFTELLDSYAFSMRDAKTAVYEKEAFFSLHKAIQTRLLYK
                     SFSQLSVHSRISYAVIEQILAKQGIETDEIIVFFDNQNIIVQKKDKREFARKGKKNFY
                     AIVEKECKFITNYATISIDRADEKTDYVKGQVIAYKKKLPFALSYDGENAKLDFFHQE
                     AEADKKYLQLRIENKRDYK"
     gene            complement(10892..11443)
                     /locus_tag="PKW26_03650"
     CDS             complement(10892..11443)
                     /locus_tag="PKW26_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOQ92696.1"
                     /translation="MEQLGLKANKRSELGKKAALKIRKEGKLPAVVYNTKGEATALTI
                     KEDEFTKIWKQATPTTLINLDIDGTVSKAFIQATDYDIISDKNLHVDFHIIDESAVLK
                     RSIKMQVAGNPVGVREGGRLEKGVKSIVISCLPKDLPVRIVADVSKLKLGDSLYVKDL
                     NLGEGVTVVSDTEDLVASVAALV"
     gene            complement(11503..11574)
                     /locus_tag="PKW26_03655"
     tRNA            complement(11503..11574)
                     /locus_tag="PKW26_03655"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11540..11542),aa:Gln,seq:ttg)
     gene            complement(11673..11954)
                     /gene="spoVG"
                     /locus_tag="PKW26_03660"
     CDS             complement(11673..11954)
                     /gene="spoVG"
                     /locus_tag="PKW26_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HOQ92697.1"
                     /translation="MQITEIRIRKVAAEGKLKAYVTVTFDDCFVVHNVKIIEGKTGLF
                     IAMPSRKTSGGDYKDVAHPISPEFRTEMQEKILAEYQAGNVEDSSPSDD"
     gene            complement(12003..12875)
                     /gene="ispE"
                     /locus_tag="PKW26_03665"
     CDS             complement(12003..12875)
                     /gene="ispE"
                     /locus_tag="PKW26_03665"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOQ92698.1"
                     /translation="MMLGELTVFCPAKLNIHLQVKEKRADNFHSIESIFQTVSLYDTV
                     RLRQVEKAGCEVFVEGMTLDSKNTLTQAYEAFSAYTGVEKGLSVSVQKIIPAGAGLGG
                     GSSNAAALIRSMDKLFETGLGEDALLDIAAQVGSDVPFFLKGGAAIVSGRGEKIRAIK
                     PRDDLIFVLIFPEVHSSTKEAYAQVDLEIEQGLAEKGPGVESLEKMYYESPESWTFIN
                     SFSNIIMSQQSLVKQAFADLQEQKSLYVQMSGAGSSVFGVFLSEDFARDAYKNLSKKW
                     KRSYILTSSTVDCC"
     gene            13148..13384
                     /locus_tag="PKW26_03670"
     CDS             13148..13384
                     /locus_tag="PKW26_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92699.1"
                     /translation="MLTEMELLEDSSMMEYGFDYAYNDDYDDDFDEDASLDDFEDDEF
                     LDEDFEDDIEDYDDEEEDFDYEDDDDFGYDVFDE"
     gene            complement(13496..13840)
                     /gene="rsfS"
                     /locus_tag="PKW26_03675"
     CDS             complement(13496..13840)
                     /gene="rsfS"
                     /locus_tag="PKW26_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOQ92700.1"
                     /translation="MKTTKEKAIEIAKLMQEFKGEAVLAMDVSQLNSWTDYFVIVTTN
                     SATHSKGLFKQIKDYIKENDLEIMVPKRKIPDGYDWTLVDLGTIVIHLMSEDAREFYN
                     LEKLWHLGETIF"
     gene            complement(13876..15069)
                     /locus_tag="PKW26_03680"
     CDS             complement(13876..15069)
                     /locus_tag="PKW26_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOQ92701.1"
                     /translation="MPTIKHRSGLLFLILIFLVIFVTSVFLIIALQTDFISESLKNDP
                     LVNTLFIITDADEILSTQIVLYYPDNKRAALFDIPGNTGAIFSSIGRVDRIDAIYAEK
                     GLAAYAKEVQTLTGLKIPFTILVDIEQFARLTDYFNGLRVFIPRPIDSQDGDTRYLLP
                     SGSVKLDGDKIQAFLRYNPEEDESSGLQEKRQDIFIAFLAALNANKEILENKKNYAVL
                     EKEFSTNLSSKDLKKILLYLSEIDSERLNGQTVTGSIKTVDGKRLLLPFYDGQLIKDV
                     CKQTINALVSSSNIVQSRVYVLEIQNGTNTAGLAKNTSSLLQSVGYEVLSVTNAANKP
                     IEKTYIIDHIGYPEMAEEIGSFIRCTNVITEEIDLESSDLAEASLVDYTIVLGTDFDG
                     RYVRR"
     gene            complement(15102..15734)
                     /gene="yqeK"
                     /locus_tag="PKW26_03685"
     CDS             complement(15102..15734)
                     /gene="yqeK"
                     /locus_tag="PKW26_03685"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOQ92702.1"
                     /translation="MLKTEVLEEIQLYMQKNMDDKRYEHSLRVADTAKILCIQYGENP
                     EAGYFAGLVHDLCKQFSDEKIKTLAKKDGLAISHIEKKKPSLLHGRAGAVLLEEEFFS
                     NELFRKNAADLLEYEDGILEAVRFHTFGEEKLSVLAKIIYIADKIEPGRSYEAKSLLE
                     EIAVLELDDLALKVIQDAIAYLERKKKEVALSTLRFYEELLSNRVADYDD"
     gene            complement(15727..16353)
                     /gene="nadD"
                     /locus_tag="PKW26_03690"
     CDS             complement(15727..16353)
                     /gene="nadD"
                     /locus_tag="PKW26_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOQ92703.1"
                     /translation="MKIAILGGSFNPVHLGHLHIAEQTQSLLGYEKIFFVPAYISPFK
                     EKPMEVSNSERIRVLKKALQYNRDFQIENIEIKRKGLSYTYDTVLYLNQKYKKKLQSE
                     EGIFQKIGLILGADLLPDFHKWHKYEKLAEMVHFILAKRHGSKIMEKDVEVQKAIEGF
                     PFPHSLLENTFMDISSTDIREKIQKGEDWQSFLPKASVKYIEKKGLYA"
     gene            complement(16370..17521)
                     /gene="obgE"
                     /locus_tag="PKW26_03695"
     CDS             complement(16370..17521)
                     /gene="obgE"
                     /locus_tag="PKW26_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOQ92704.1"
                     /translation="MVKFADEALIEVRAGNGGNGCVAFRREKFVPRGGPAGGDGGKGG
                     DVLFCIKRNLRTLAHLRYKRVFKAKNGADGQGSQKYGRDGEDVLIPLPPGSIIKDAET
                     GEIIHEFTTETEAEVFTFLTGGKGGWGNVHFKSSTNQAPRTAYEGSLGETRKLKIELS
                     IMADIGLVGFPNAGKSSLLEYFTNARPKIAPYPFTTKIPNLGVLRVSDEKDIIIADIP
                     GIIEGASSGVGLGFTFLKHISRTAGLAFLIDCSDEQYLTAYNTLLYELKTYSKDLVQK
                     RRIVLLNKIDLPGAAERAAELAQILKKEHPLDIILPISIKKKEGMGRVQEEMEAMVER
                     MEENAALQTETSMGYTYVNYTQSQKKSSFLDSRASCSDETHQFPGSENE"
     gene            complement(17632..17889)
                     /gene="rpmA"
                     /locus_tag="PKW26_03700"
     CDS             complement(17632..17889)
                     /gene="rpmA"
                     /locus_tag="PKW26_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOQ92705.1"
                     /translation="MARKKGGSGSKNGRDSNPKYLGVKVYGGQIVPAGSILVRQRGTK
                     IHPGVNVGKGKDDTLYATETGTVVYHERKGRKLASVSPLEA"
     gene            complement(17908..18234)
                     /locus_tag="PKW26_03705"
     CDS             complement(17908..18234)
                     /locus_tag="PKW26_03705"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOQ92706.1"
                     /translation="MRLAKTKRGSLRYCSSSGHAFFARKGSDIVCAAVSTLLRTTLLL
                     LQEKKDLEVEVSLQKRGELFFKIINAEDEASLDFLQFAYAFLEKGLQSIEKDYPKHLS
                     IAFFIE"
     gene            complement(18246..18560)
                     /gene="rplU"
                     /locus_tag="PKW26_03710"
     CDS             complement(18246..18560)
                     /gene="rplU"
                     /locus_tag="PKW26_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOQ92707.1"
                     /translation="MYALIEYKGKQYKAEKDALITVDKLDIEKGQNIEIDTVLLVSDE
                     KNVSVGTPYVKGALVKAVVEDSFKDKKVIVYKHKSKKDYYKTQGHRQQYTKIRVSDIV
                     VA"
     gene            complement(18662..19828)
                     /locus_tag="PKW26_03715"
     CDS             complement(18662..19828)
                     /locus_tag="PKW26_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HOQ92708.1"
                     /translation="MKKDDYRLKRYAEAAQTSKVELQQPLIKTAQSAKDSLTKTLDEL
                     TKQGLVKVPLPSKTEEKESIFRRVAKFLLIIGIDEAAKILPHLLPEQVDKIALEIASI
                     RSVDEDEAALILAEFESLVQKSRESGGVETAYEILEKAFGPERAEALIQKKIAFPKGK
                     PFEYLQEADTEKIQQLLHDESDAIRSLVLSQLPPKRAADYIQALPQAEKTSLVHRLAT
                     LNKIDTEVLVRVDSAMREKTQRLSIEKADSIDGKAALVEILKKMPGSGDLRILSSIEN
                     SDPDLSREIKEQLFSMTDILKADDRFIQKKLQEMEDASIAYLIAGKSETFRLKILGNI
                     SKGRKALVLEEENMRKPMKKRDVDEVTLAFFTLLRKAWENGELILHDSSDILVY"
BASE COUNT         6098 a         4050 c         3443 g         6269 t
ORIGIN      
        1 attggtctaa tcttagaaat tgtgcttata ttcaagaatt tgaaaaagag aagatagcct
       61 ggaataggat agcaagcaaa aaactttttt ctttaattga cgaggggatt atcattcaag
      121 atagtatgca cttttttaca ggtaatcatt tgaaattttt atgtgcaatt ttaaattcaa
      181 aattattttc ttggtttatg tatctaatca tcggtgatgc tgctggaggt aatgcaggaa
      241 atgctgataa tgttagtaat ttaactatta taaaaccaac agagcaactt gaattacaaa
      301 ttgaaaattt attaaaaaat gaacaatatt ctgagattga taaaattatt tatcaattat
      361 atgatttaac agatgaagaa attgcattta ttgaaaaagc gagtaaataa acagatcccg
      421 aaattttaaa gcctattttg gaaagctaac aaagggcatg acattcactc ctatcatcac
      481 cgctctttaa aatgacttgt ttttttacga ctatgtatga aaccttcaag acacagggga
      541 gaccatgatt ttttttccat agattgacag agcaagcccc tcatgataaa ataatagaat
      601 atgcatctta aataataagg aatcgttcta ttatggataa caaaaaaaca atcaatacaa
      661 aaaagacaca aaaatctggc acgaagctct tttacaccat tggctctatc gttattttag
      721 ttttagctgc cctcgccttt gttcttatcc ccgcccttac cactacaggt agcagagatg
      781 aaattatcgt aggaagctgg aataaaaagc ctataaaata tgtagcaaat tccgacttca
      841 ccaaaaatgt cgaatactat agcaattact acaagcaaca gggctatgaa gtaaatgatc
      901 aaaactttta taccattctc gaccatgctt ttaccgcaac tgttttaaac agagcaatta
      961 tcgatgaggt tgataaagca ggttatatac cttctcaaaa caccataaac agagagctcc
     1021 tcccctattt ttacgatact gaaggaaact attccgcaaa aatctttcga gatactcctg
     1081 atagcaaaaa aatcgaatac aaaaaaaata tcattgaaaa cctaaaatac ggaagctatg
     1141 tcatggatca ttttgcgcaa acaaaggttc cctactacgg tttaaaatct gcaaaaaatg
     1201 aaagtgttgc tttggcaaag atgaatgcct acaaaaaagg ctttgactat gttgcatttt
     1261 caacagacga ttatccacta tcacaagcac gcaattttgc aaacgaaaac cctgatttat
     1321 ttaccgaata caatttttca gttatttctt ttgacactga agcacaagca aaatctgtac
     1381 gaaagcaact cgaacaaaac gaaataacct tttttgatgc tgtggaaaac ttttctacaa
     1441 aaaattatag cggggatgat ggtattttta cttataccta tcagtatcaa ataaaaccgc
     1501 tactcgcaag tgaggaagag ctcaaatccg ttttggcaac tgaagtagat agcctcagcc
     1561 ccatcgttaa aacgtcaaat acctactcaa tctttaaggc aaacgcaaac ccaaaacaag
     1621 ctgattttac aacagacgca agcctcgata ttgttttaaa ttatttaaaa caatatgaaa
     1681 tggggcgcgt acaggattat tttataagcc ttgcaaatga tttttctctc aacgcaagtc
     1741 tcggaaactt tgcaggagct gcagaaaaat ttaacagaga agttaaatca ctcgagccct
     1801 ttgcattgaa ctatggcaat tcagaattac tctatgcagt acctagttct agcgatggcc
     1861 tactctacgg cgcagaaaga aatgaaagtt tcttaaagac cgctttttcc ttggaagaag
     1921 gcgaggtttc tgcccctatt attctcggtt caaatgttct tgttttacaa tgcactgaag
     1981 aacaaaatga gctaagcgaa agtgagcttg aaaattatac atatatctat gcatattacg
     2041 ctggacaata taacgaaaat tctttttata attatttttt aagtagcgat aaaacagtaa
     2101 acaatgtttt tgctacctat ctaaagtatt tttaccaata aactgaggat acctagatga
     2161 gccctgataa gcatagcaac aaaaggcttt cctcagggca gccctcttta gaacgagagg
     2221 atgcagatat acaaaagatt gaaacgaaaa acggttttac tgttttatat aaaaatcgct
     2281 acttatattc tcgctttgat gcaaaaaaaa attcagagag gctcgtgcaa tctttagaaa
     2341 taaaaggtga gagcctcttt tttatttttt caccacttct cggtcatggc ttagacttat
     2401 tgtttgaaaa aatatcgagc gattcttttg tcttagctat tgaaacagat cccctcctct
     2461 atgccttttc actacaacac atcaacaaaa aacttctaga aaaaaagaat tttctctatc
     2521 tctgtctcga agaaaaaatg acgggagtaa aagagctact agaaaaaata gaccatcttg
     2581 tacagagcga ggaaggctac gcttttagac gtcttgtacc actggaactc agtagcactt
     2641 tcatacaaaa agaatttcaa aatacctgca cgcaatatat agatttgtac ataaatcaat
     2701 tttggcgaaa tagaataacg cttgttaaac tggggcgact ctatgcaaaa aatatctttc
     2761 aaaatcttgc cctcctatcc caggcagaga caataaaatc tgcgacaata gaaaaaccca
     2821 tccttgtttt aggagcaggt ccctcgctcg aagaaaatat tgcaataata caagcatttc
     2881 aaaagcactt ttttataatc gctgtcgaca ccgctttggc atatctacac aaacaaaaga
     2941 taggcataga cctctgcctc gtcatggaac cacagttgat aaaccaaaaa gcctttctcg
     3001 ctgtaaaaaa cacaaagata ccgcttctcg ccgatctttg tagttatcct gcaacacaaa
     3061 aaatccttaa tggaccgctt tcgtttttct tttcagaata tgcttcactg caatttttac
     3121 aaaaccttgc aaaaaattcg ggcattcaaa aatacagggc gcttggttcc gtaggtttag
     3181 ttgcggtaga aacagcttta aaactgcgta aaaaaaacac tgcaatcttt tttacaggct
     3241 tagatttttc ctaccagctc gggaagacac atacaaaaga gactccttct catctcgccc
     3301 gattgttcga ggcaacacga ctcaagcctg cagagaatat tgggcttgtt gaaaacaaga
     3361 taggagacgg actctataca aatcccgctt tggaaaatta tgcaaaactt ttcgatgacc
     3421 tctatgcgga ggaaaacgaa ttatacatgc tctctgaaac aagcattatt cgaaaaataa
     3481 aaaaaatctc tcttgaggat tttagaaaat ggatagaaaa taaggccagt caaacagata
     3541 ctagtcaaag agattctata gaaaaaaacg aaaaaataga gtcgcgtttt tttgaaacag
     3601 atactgaaac aaatactata gaaaaaacaa aaagaaagaa tgcgattatt gatttcttgg
     3661 aaaatgaaat aaaaaagctc tctgttttat cgagctttct agaaagcata aaaccagagc
     3721 aaacaagttc tcagctagat gaaaatcttc aaaaggaagt aagaaaaaag cttctcggct
     3781 tagaatacct ttacatacat tttccagaag catacaaaaa accggatata catttctcct
     3841 ttatctcagg gctaaaagga gaaatatctt tttttctaaa aatcatcgaa aggtctctcg
     3901 ataggattac gagtgagcat ttttaaaaat ggttttttga aaaagcgttt atttattctt
     3961 cttttgtttt aagcaccgca aggaaggctg attgtgggag ctcgacatcc cctaccatct
     4021 tcattcgctt ttttccttcc ttttgttttt caagaagttt tttctttctc gttatatctc
     4081 caccataaca ttttgccaaa acatctttac gcaaggcaga aacagtttca cgtgcgataa
     4141 cttgactccc aattgcacct tgcaccgcaa ttttaaattg ctggcgggta atttcttctt
     4201 ttaactgcgc acaagtattt cgcgcctttg caacggcatt tcccttatag gtcaattgtg
     4261 ccaaagcgtc cacacttttc ccatttataa gaatatcgat ttttacgagt tctgtttctc
     4321 tataaccgat gagctcataa tcaaaggaag catagcccct cgtataactt ttgagtcggt
     4381 cgtaaaagtc gaagagtact tcggcgagcg gcatctcata aaatatttca acacgctttt
     4441 catcgaggta ttgcatattt gtttgtacgc cgcgtttttc tgtgcaaagc gccatcacat
     4501 tccccaagta tgttgcaggg gtaataattg ccgcctttat atagggctct tcagattttg
     4561 ctattttatt gagttcagga aaatctgcag gattatcgat atatttttct tctccatttg
     4621 aaagagtaat aagatagcga actgagggag cggtaaaaat aacagattgg tcaaaatctc
     4681 tctctaagcg ctcttgtaca atttccaagt gtaagagtcc caaaaaaccg cagcgaaagc
     4741 catgcccaag tgccatcgaa gagtcttttt caaaaactaa acttgcgtca ttgagcttga
     4801 gtttatccat gctttcgcga agctcttcat aatcgctcgt atcaacagga tagatggatg
     4861 aataaacaac gggcttaaca tctttaaatc cgtcaagtgg tttgtcaaca ggattttttg
     4921 tcagtgttac tgtgtcgcca acctttacat cggaaactgt ttttatacca gcaatgatat
     4981 agccaacgtc gccacattca agggaatttt tttcaactaa gccaagtcga aaaataccgc
     5041 attcttcaac cttgtagtca gactcagaaa ccataaaccg aacagtgtcg ccttttttta
     5101 tggagccaga aaaaatacgc agatgcacaa cgacaccacg atagggatcg tagtggcagt
     5161 caaaaataag tgctttggta aaaccagatt cgtcatcact cggtggtgga atttgattta
     5221 caatagcttc aaaaagctca tcaactccct cgcccgtttt tgcagaaata caaacagcca
     5281 aatcgggatc aagccctaag tcatggtcta tctgtttttt tacgcgtggg atatctgctg
     5341 caggtaaatc aatcttgttt ataacaggca ccatttctaa attttgctca agtgcaagat
     5401 acatatttga gagggtttgt gactcaactc cttgagcagc atcaacaatg agcaaggcgc
     5461 cctcgcaaga agcaatagca cgagaaactt cgtatgaaaa gtcaacgtgg ccaggtgtat
     5521 caacaaaatt taactcatag agtaaaccat cttttgctgt atagggtatt gtaaccgcat
     5581 gactttttat agtgatgcca cgctctcgtt caatatccat attgtcgagc atttgatttt
     5641 gaaaaacgcg atcatctaca acttttgttt tttgaataag tctgtcagca agggttgatt
     5701 tcccgtggtc aatgtgcgca acgatgcaaa aatttctttt ttgtactaat ggtgtcatgg
     5761 ccctagtata ttaaaaaaaa taaggactgt ccagactcgc ccctattgcg aggtttactt
     5821 caaaataggc tttatttctc ttgcgtgtaa aaagctctgc atagacaatc gtgttttttt
     5881 ctaaaaattt tatttttttt atttcaacag ggtcttgtag agaaaatcca ttttcatcgg
     5941 cagcactacc ctttataatc gaggctattt cgtattgatt tttattgtat accgaggtgc
     6001 gctttaactt catcccaaaa cttggtagaa aaaaatcttc gacaggctct cttttatagg
     6061 cttcgtaaat aggcatttca gggcgctctt ctgtgtaaag atattttgta aaagcttctc
     6121 cctcttggtt tactccccca agctttataa ttgtccctgg cattatttcg agcattgcat
     6181 tttgatagtc ttcgacagtt cgtattttcg tagcgtttag agattgtaca atatcgccct
     6241 tagccaataa gcccttttga gcagcacttc cacgcataac ataatgcact tcgataccca
     6301 gggcttcttg ttgaggaagc tctctttttg tttttgcata aaagcctgtg tacgaatgtt
     6361 tcacttcgcc gtgaaaaagc aaaactaaaa tcttttcgag ctgttcaact ggtatcgcaa
     6421 aatttaatcc ttcgtactca agcacgccag caaagacaat tgcttgcaca agtccatttt
     6481 gatcaacaat cggaccacca gaatttcccg gattaatagc tgcatctatt tgcatagcgg
     6541 aagctactgt agaaagcgag cgactcttgc tagaaactat gccgcttgta atggtgttct
     6601 caagaccgag gggacttccc atagcaaaaa ttctatcgcc gacttcaagg tcctcagatg
     6661 agcctaggga aaaagaaaaa gggacaggca cctctgtttt caaaagcgct aagtcgagcg
     6721 ccttatcgta accaataacc tttgcaggat agcgagtttc aacatcgtca tagagcttta
     6781 cataaacccg agaatatccc ttgtatgtcg tatcaacttc actttcaata acatgatagt
     6841 tggtaatcat atgtcccatt gaatcaataa aaaaaccaga gcctataatt ctatctggca
     6901 gcccgagccc tctttgtacg gaaactccac ggtcaaccca aacggtaacc aagccttgca
     6961 aatactgtga aaccttcgat ggaggaagag agggaaacgg ttcgctatac gcattgtcag
     7021 aatttttgga atctttcaaa gcgtgattat tctcgtctaa gcctagggct ggaaaatatg
     7081 tttttagaaa atcattttcc aagcgctcca gttcatttgt gtacaaagca atttcagtaa
     7141 cagaaagatt ttgcaaggat agaacaaagg cttttgcaag tggaatattt ttttcatcaa
     7201 gtgctgtctg cgtttctctc aaaacaaaac ttttagcttc atcaaaaaca gcttcgcagg
     7261 cgtctttgtg ttcatttgga aatccctcta gagtctcaag caataaggct gaacgccaaa
     7321 gcgattcaag cggcttctct tctatttgca aacgaatctc gtctatttct ccattgcgtg
     7381 catcgagttc tgtgtaggca agcggaataa aatgatgctc ggaatgtgtt tgacaagcaa
     7441 gaataaaaaa agagagtata agaaaaaaag gatgaaaaag cttagtagat agtttcgtct
     7501 tcaatgagtt ctccaaaata aaaagtatct attttaatag caagcattct aaagccgcgc
     7561 atatcatgat catcgatgag aatacgcatg ccctgtatat ctgtttttgc aagctcagta
     7621 taggccttga gcgcatggcg agtataggat gattccccaa gccaataaaa aggtaggtct
     7681 ttttctttta ggattttttt tgtgcgatct gcataacgag caattgtcgg tatcccattt
     7741 tcaagaacaa tttccacttc ttcattgtta agtggattta taaaaaagac cttctcttct
     7801 aagaaatcac ctgcttgcat atttttttca taggctaggt cccaggagcc attttcgttt
     7861 tcatcccagg ctgttatttg tctgccatca ggaaggtagc tttctcgaaa atctggaatg
     7921 ccattttcat ttgtgtcaat acgcacctct gaaagataat agccttctgg catttcaaaa
     7981 cttccaaaca gttccgtgta gagatttgct tctttatttt tctctgattt aagctcgggg
     8041 tcaaaggcat aaaattcagt tgtttcatag ctaccgttcc cgtctctgtc aacatttcga
     8101 aactgcagta agccattttc aaaaaagccg taggcatagg gtttatcttt aagcgtatat
     8161 aaggctgttt ttatacgatt gttttgcaaa ctaaagcgga ttttcttatg ctcgttttct
     8221 ttcggataga taactatttc gtttgcaaag gcttgcatcg ttcttaaatc aaatgcttga
     8281 ctatctctat ttaataccgg caaataaaaa accttgttct caaaaatcgg tatcagttct
     8341 agctcgtagg cttcccaagt aacagatgca tcgacaaagc gcatttccat ccgttcttct
     8401 tccaaagcaa agctagaaaa agaatcaaca aagggatagc gaccaaaata cacttcatag
     8461 gatttttcgt agtctataca tttttcagca agtccataat cgcattctat tatccagttc
     8521 aacgcatcgt cagcgttttt gtcatagaca atcgtagaag gtcttccgta cttatagtag
     8581 acgaaaaaat catctgcccc atccctattg ctatcaaagt ctaaacgccc ggaaaaatcc
     8641 tcgagatttt ctgcaaaaaa agcaaaagac tgtggcgcct ggaggaccga gagcaactca
     8701 aaaaacaccg gagctgctat gcccctatct gcaatctgaa acaaagcttg tattgcctgc
     8761 tcctcggtat ataaaccatg ctctaagaaa gcaagaatat agagagggtc ttttttttcc
     8821 gtcagccgat attctttgag ataacgtacg gcgctatcct catccacaaa aaatgatgcg
     8881 tagaccaaga tatcgggctc ccttgttttc catttttcta gcatagctaa aagcgatgta
     8941 ctaagcgctt gaaatgcttc ttcgttactt tgcatcaatc gttcattttg aaaaaacaaa
     9001 agcggaaatc gagcatcgtt tggaaataaa cgccgtgcat tggaaataat ttcatgcgcc
     9061 ttttctctgt cctgcaagcg ataagaagca aggcaacgaa ggtattcggc atcactttga
     9121 attaaaacag gtttttcatc gaggattttt agagcagcgt gcatatctcc tgttgcaagc
     9181 gcatattttg catacaaaat acgtgccgct tctggggtat aatataacca agattttttt
     9241 tccaaggctt tttcaactga gtcaataatt ttaaaaattt ctttattttg atattctagc
     9301 aacaaggcat gaagatacca gaggtcggaa atactctcat cataaaataa accaacttca
     9361 ataaagtcaa atgcgtctat ataattccct gcgacaagtt ggtcacgagc aagttcaaca
     9421 caacgcgtcg ctgtttgaaa atttgcctgc tgtaaataat ccgattcttg cgctccaaga
     9481 aaaagaaaag agaatagact tagtaaaaaa agagccgctc tatttttttt cacaaaccac
     9541 ctcatacttt ttatttttat cgatacagta tgtaccgaaa tatttattta taatccctct
     9601 tgttttcaat acgcaattgt agatattttt tatctgcttc agcttcttga tgaaaaaaat
     9661 caagctttgc attttctcca tcataagaca aagcaaaggg gagctttttt ttatatgcta
     9721 tgacttgccc cttcacatag tctgtttttt catcagccct atctatagaa attgtagcat
     9781 aatttgttat aaatttacat tctttttcta cgatagcata aaaattcttt tttcccttcc
     9841 ttgcaaattc tcttttgtct tttttttgca caataatatt ttgattatca aaaaaaacga
     9901 tgatttcatc cgtttcaata ccttgctttg ctaaaatctg ttctatcact gcataggaaa
     9961 tacgcgaatg aacagaaagc tgtgaaaaac ttttatacag aagtcgtgtt tggattgctt
    10021 tatgcaagga aaaaaaagct tctttttcgt agacagcggt tttggcgtct ctcatagaaa
    10081 aagcatagga gtccaaaagc tctgtaaaaa aatcttcatc tatcgcctgt ttttttgccc
    10141 cctgcaagag agctttttgc caaccccaaa agtacgtatt caaaaaaaca actaagcgat
    10201 ttcggatttt atttcgcaaa aaaactgttt ctgcatttgt tttatcgatg caataggcga
    10261 tattgttttt ttctagatag tcttctattt catcgcgact acaatcaaga aggggtttta
    10321 catagtttcc acgcattatt tccataccgc ttcttgcaag tgagccttga agaaagcgct
    10381 ctagaattgt ttcgagcttg tcgtttttgt tatgtgcggt aagtatgtat gaagcgccct
    10441 gctcctttgc agagtcttcc aaaaaagcat agcgataaaa acgagctgcc tcttcaagcc
    10501 ctttttttct ttttgcgcta agttttttta taaaacctgg ttcggctgtc tttacaaggc
    10561 aaagaattcc aagctcttta caaaagtctt ttacgaagtc aaggtctttt tggctttctt
    10621 tttccgatcg cagattatga ttgagacttg caacgatgat tttttgattt cgcagttttg
    10681 catatcgcgc acagagggaa agcatgcaga tagaatcagc gcctcctgaa accgctaaca
    10741 agagggtcgc atctaaaggt aaggaagcta gagattggat gagtttttct tcgatagatg
    10801 ttttttttgt ttttgacatt acataagatt gtaacataaa aaaagactga tgattagacc
    10861 atcagtcttt tgtattcact ataaaaaatt cttatacaag cgctgcgaca gaagcaacaa
    10921 ggtcttcagt atcgcttaca acagtaacgc cttcaccaag atttaagtct tttacataga
    10981 gtgaatcacc cagtttcaac tttgaaacgt cagcaacaat tcgcactggt aaatcttttg
    11041 gtaagcagct aattacgata gattttacac ccttttctaa tcgtccacct tcgcgaacgc
    11101 caacagggtt tcccgcaact tgcattttta tagagcgttt taaaaccgct gactcatcaa
    11161 taatgtgaaa atcaacatga aggtttttat cgcttataat atcgtagtca gtagcttgaa
    11221 taaaagcctt acttactgtc ccgtcaatat ctaaattgat aagcgtagtc ggtgtagcct
    11281 gcttccaaat ttttgtaaat tcgtcttctt taatggttaa agcggttgcc tcaccctttg
    11341 tgttatatac aacagcaggt agtttgccct ctttacgtat tttaagtgca gcttttttac
    11401 caagttctga gcgtttgttt gcttttaagc ctaattgttc catttttcta gaactcctta
    11461 tactagtaca ttatagcttc atagctgagc taaatagatt ttttgggacg aaaggattcg
    11521 aacctttgaa taccggtacc aaaaaccggg ggcttaccgc ttgccgacgt cccaataaat
    11581 gtgattaata aacagtatat taaaactttt agacttatgc aataggtatt tttacaaaag
    11641 ttcgataatt tgtaatatat ttagacttta gcctagtcat ctgaaggaga tgagtcttct
    11701 acatttcccg cttgatactc tgcaagaatt ttttcttgca tttctgttct aaattctggg
    11761 ctaatagggt gtgcaacatc tttgtaatcg cccccactcg tttttcggct aggcatagct
    11821 ataaaaagcc ccgttttgcc ttcaattatt ttgacgttgt gtacaacaaa gcaatcgtca
    11881 aacgttacgg ttacataggc ttttaacttt ccttctgctg caacctttct gatacgaatc
    11941 tcggtgattt gcatattcgt cctccatata ctaagtacaa aattttagtt aggtactcac
    12001 aatcagcaac aatcaacagt ggaggaagta aggatataag acctcttcca ttttttactt
    12061 aggtttttgt atgcatcgcg agcaaaatct tcactcaaaa aaacaccaaa aaccgaagag
    12121 ccagcaccag acatttgaac atacaaggat ttttgttctt gtagatcagc aaaggcctgt
    12181 tttactagac tctgttgact cataattata ttagaaaatg agtttatgaa ggtccatgac
    12241 tcagggcttt cataatacat tttttctaaa gactccactc ccggtccctt ttcagcgaga
    12301 ccttgttcaa tttccaggtc gacctgggca taggcttctt ttgtagagct atgaacctcg
    12361 ggaaaaataa gcacaaaaat caagtcatct cttggtttta tggctcttat tttttccccc
    12421 cgaccactta caatggctgc cccacctttt aagaaaaaag gcacatcact gccaacctga
    12481 gcagctatat ccaaaagggc atcttctcca agccctgtct caaaaagctt atccatgctt
    12541 cgaataagcg cagcggcatt tgaagaacca ccgccaagtc ccgctcctgc aggtattatc
    12601 ttttgcacag aaacagaaag acctttttct acacctgtat atgcactaaa agcctcgtag
    12661 gcttgtgtaa gcgtgttttt tgagtctagg gtcataccct cgacaaatac ctcacatcca
    12721 gctttttcaa cctgccgtag cctaacagta tcatacaaag aaacagtttg aaaaatgctt
    12781 tcaatcgagt gaaaattgtc tgctcgtttt tcttttacct gcaagtggat atttagcttt
    12841 gcaggacaaa agacagttaa ttcacctagc atcatatata attatagcaa ataataatgc
    12901 tttgtcaaac attttaaaag gctttttaaa aaaactcctc tttctcaaaa aacgaaaaga
    12961 gtagaaaact atggaaaata aagccttttt atgcattttt atacatattt gtttgcgtat
    13021 aaaaaaaata taaaatattt tacaaaaaaa gataaaaaaa cactcgtttt tcgttatcaa
    13081 gtagttgaca tttgattagc tgtattttat gttttaaata tgaaagacaa agcaaggaag
    13141 tatttgtatg ttaactgaaa tggaattatt ggaggattca tcaatgatgg aatatggatt
    13201 tgattatgca tacaatgatg actacgacga tgattttgat gaggatgcct cgttggatga
    13261 ttttgaagac gatgagtttt tagatgaaga ctttgaggac gatatcgagg actatgatga
    13321 cgaagaagaa gacttcgatt atgaagacga tgacgacttc ggctatgatg tttttgacga
    13381 ataagctcct acttgagcta tacgttaaag catagaagat gaaaaaactc ttgtaaaatt
    13441 tttacaagag ttttttactt ttaattactt tttatccaaa agaaaaaaaa ccaaactaaa
    13501 aaatagtttc cccaagatgc caaagctttt cgagattgta aaactcacgt gcatcttcag
    13561 acatgagatg gataacaata gttcccaagt caacaagggt ccagtcatag ccatcgggaa
    13621 tttttctctt cggtaccatt atttctaaat cattttcttt gatatagtct tttatctgct
    13681 taaataagcc ctttgaatgt gttgcactat ttgttgtaac aatgacaaaa taatctgtcc
    13741 agctattcag ttgtgaaaca tccattgcaa gcaccgcttc tcccttaaat tcttgcatga
    13801 gttttgcaat ttctatcgct ttttcctttg tagttttcat atgtccccca taatttattt
    13861 ttataatgct aatagctagc gtcttacata gcgcccatcg aagtctgttc ccaaaacgat
    13921 ggtatagtct acgagagaag cttcagctaa atccgaactc tccaagtcta tttcctcggt
    13981 tatcacattt gtgcagcgaa taaaagagcc tatttcctct gccatttctg gataaccaat
    14041 atggtcaata atatatgttt tttctatagg tttattggct gcgtttgtaa cactcaaaac
    14101 ctcatagccg acactttgaa gcaagcttga tgtatttttc gccaagccag cagtatttgt
    14161 tccgttttgt atttctaaaa catagactcg actttgtacg atgtttgaac ttgaaacaag
    14221 cgcattgatt gtctgtttgc agacatcctt tataagttgc ccatcgtaaa aaggcaaaag
    14281 caatcgctta ccatcaaccg ttttaatcga acccgttacc gtttgtccat tcagtcgctc
    14341 actgtcaatt tcagataaat aaagcaaaat cttttttaag tcctttgaac taaggtttgt
    14401 agaaaattct ttttccaaaa ccgcataatt ttttttattt tccagaatct ctttatttgc
    14461 atttagagca gctaaaaaag caataaaaat atcctgtctt ttttcttgca aacctgaact
    14521 ttcatcttct tccggattat atcgtaaaaa ggcttgtatt ttatcgccat cgagttttac
    14581 cgagcctgag ggtaaaagat agcgcgtatc tccgtcttgg ctgtcgatag gccttggaat
    14641 aaaaacgcga agcccattaa aataatcggt aagtcgcgca aactgttcta tatcgactag
    14701 aatggtaaag ggtattttta agccggtaag ggtttgtact tcttttgcgt aggctgctaa
    14761 acccttttcg gcataaatcg catcgatacg atcaacacgt ccaatcgatg aaaagatagc
    14821 ccctgtattt ccaggaatat caaataaagc ggctcgttta ttgtcaggat aatacaagac
    14881 aatttgagtg ctcagtatct catcagcatc tgtgataata aacaaggtat ttacaagggg
    14941 atcatttttt aatgactcac tgataaaatc tgtttgcaag gcaataatta aaaaaacgga
    15001 agttacaaag ataacgagaa aaataagtat gagaaaaagc aagccactgc ggtgtttaat
    15061 tgtaggcata aaccatcctt atcattatat gtattttagt tctaatcatc ataatcggca
    15121 actctattgc tcaataattc ttcgtagaat cgcaaagtag aaagcgccac ttccttcttt
    15181 tttcgctcca aataggctat ggcgtcttgg ataactttca aagccaaatc atctagctcc
    15241 aaaacagcta tctcctcaag aagcgatttt gcctcataag agcgacctgg ctcaatttta
    15301 tcggcaatat agataatttt tgccagcaca gagagttttt cttctccaaa ggtatgaaaa
    15361 cgaaccgctt caaggatccc atcctcgtat tctaaaaggt ctgcagcatt ttttctaaaa
    15421 agctcgtttg aaaaaaactc ctcttctagc aaaaccgctc ctgcccttcc gtgcaaaagc
    15481 gatggcttct ttttttcaat atgcgaaata gcaagcccgt ctttttttgc caaagttttt
    15541 atcttttcat cagaaaattg tttgcaaagg tcatggacta aaccagcaaa atatcccgct
    15601 tctggatttt cgccatactg tatacaaagg atttttgcgg tatcggcaac gcgcaagcta
    15661 tgctcatagc gtttatcgtc catatttttc tgcatataga gctgtatttc ttctagaact
    15721 tcggttttaa gcatagagcc cctttttttc gatgtatttt acactcgcct taggtaaaaa
    15781 actctgccag tcttctccct tttgtatttt ttctctaata tcggtactcg aaatatccat
    15841 aaaagtattt tccaaaaggc tatggggaaa aggaaagcct tcgatagctt tttgaacttc
    15901 cacatctttt tccattatct tcgagccatg tcgctttgca agaataaaat gaaccatctc
    15961 tgcaagcttt tcatacttat gccatttatg aaaatcggga agtaaatctg caccaagtat
    16021 taagccaatt ttttgaaaga tgccttcttc gctttggagc ttttttttat atttttggtt
    16081 taaatataaa actgtatcgt aggtataaga aagtcctttg cgttttattt caatattttc
    16141 tatttgaaaa tccctattgt attgaagggc ttttttgagc acccgtatac gctcactatt
    16201 gctcacttcc atcggttttt ccttaaaggg tgagatatag gctggcacaa aaaaaatttt
    16261 ttcatagcca agaaggcttt gtgtttgttc agctatatgc aaatgcccga gatgtacggg
    16321 attaaagcta ccaccgagga ttgcaatttt catttaaaag ccctcgccct cattcattct
    16381 cgcttcctgg aaactgatgc gtttcatctg aacaagaagc gcgtgaatct aagaaactgg
    16441 attttttttg tgactgagta taatttacgt atgtatagcc catagaagtc tctgtttgca
    16501 aggcagcatt ttcttccatt cgttcaacca tagcttccat ttcttcttgc accctcccca
    16561 ttccttcttt cttttttata gaaataggca gaataatatc taagggatgt tcttttttta
    16621 agatctgcgc aagttctgct gctctttccg cagccccagg aagatctatt ttgtttaata
    16681 atacaatacg tcgcttttgc accaaatctt ttgaataggt tttaagttca tacaaaagtg
    16741 tgttataggc tgtcaaatac tgctcgtcag aacagtcgat taaaaaagct aaacctgcgg
    16801 tacgagaaat atgctttaaa aaagtaaagc ccagtcctac gccactcgat gcaccctcga
    16861 taatccctgg aatatctgcg atgataatgt ctttttcatc tgaaacacgc aaaacaccaa
    16921 ggtttggaat ctttgtcgta aagggatagg gagcaatttt tggtcgggcg tttgtaaaat
    16981 attcgagaag ggaagatttc cccgcgtttg gaaagccaac aagcccaata tcagccataa
    17041 tgctaagctc gatcttgagt tttcgcgttt cccccaagct cccttcgtag gcggttcgcg
    17101 gcgcctggtt tgttgaagac ttaaaatgca cattacccca accaccctta ccgcccgtta
    17161 aaaaggtaaa aacttcagct tcggtttctg tagtaaactc gtggataatt tcaccagttt
    17221 ctgcatcttt tataatactt cccggcggta ggggaatgag tacatcttca ccatctctgc
    17281 cgtatttttg agaaccttga ccatcggcgc cgttttttgc cttaaacacc cgtttatagc
    17341 gtaaatgcgc cagagtgcgt aagtttcgtt taatacaaaa caaaacatcc ccacccttgc
    17401 caccatcgcc cccagcagga ccacctcgag gaacaaattt ttcacgtcga aaggcaacgc
    17461 aaccgttacc accattaccg gctctaactt cgatgagcgc ctcatctgca aactttacca
    17521 cacaaacctc atttttttag tcgatacaca aaataataaa aaaaataccc ttatggaatg
    17581 actttgagcc tccaaagggt atctgcgtag aagataaaaa aagagtttcg gttaagcttc
    17641 taaaggactt accgatgcta attttctacc ctttctttca tgatatacaa ctgtgcctgt
    17701 ttctgttgca tagagcgtat catctttacc ctttccaaca tttacaccag gatgaatttt
    17761 agtgccgcgt tgacgaacta aaatagaacc tgcaggaaca atttgtccac catatacttt
    17821 tacacccaaa tactttggat tggaatctcg accgtttttt gagccacttc cacctttttt
    17881 tcgtgccatt atatgcctcc gtctttttta ctcaataaaa aatgcaatgc ttagatgctt
    17941 tggatagtct ttttctatac tttgcaaacc tttttctaaa aaagcatagg caaactgtaa
    18001 aaaatccaaa gatgcttcat cttcagcatt tattatttta aaaaacaact caccgcgttt
    18061 ttgcagtgaa acctcaactt ctaaatcttt tttttcttgc aaaagtaaaa gcgttgtgcg
    18121 caaaagcgtt gataccgctg cacagacaat gtcgcttccc tttctagcaa aaaaagcatg
    18181 acctgaagac gaacaatacc gcaggctccc tctttttgtc tttgcgagcc gcacctgtat
    18241 catgcttatg caacaacaat gtcagaaaca cggatttttg tatactgttg tctatgtccc
    18301 tgtgttttat aataatcttt tttgcttttg tgcttataca caatcacttt tttgtcttta
    18361 aaactgtctt ctacaacagc ttttacaagt gcacccttaa cataaggagt gccaacactt
    18421 acatttttct catcgcttac gagaagaaca gtatcaatct cgatgttttg tcctttttca
    18481 atgtcaagtt tatctacggt aataagggca tctttttctg ccttgtattg ctttccctta
    18541 tattcgatca gtgcgtacat ttcatacctc tatataatga attcgtctaa acagtataca
    18601 attttttata aaatctagtc aatagatgaa aaaaaaatta taaaaacttt gaaaagagcc
    18661 atcaatacac aagaatatcg ctgctgtcat gtaagataag ctcgccattt tcccaggctt
    18721 ttcgcaaaag cgtaaaaaaa gcaagcgtta cctcatcgac atcacgtttt ttcatgggtt
    18781 ttcgcatgtt ttcttcttct aaaacaagcg cctttctgcc cttggaaata tttccaagaa
    18841 tcttaagccg aaaagtctcg gactttcctg ctataagata ggcaatgctt gcatcttcca
    18901 tctcttgcag ttttttttgt ataaacctgt catcagcctt taaaatatcg gtcatgctaa
    18961 aaagctgttc ttttatctcc ctgcttaaat ccgggtcact attttctatt gaagataaaa
    19021 tacgaaggtc acctgaacct ggcatttttt ttagaatttc taccagcgca gcctttccgt
    19081 cgatagagtc cgccttttct atagaaagac gctgtgtttt ttctcgcatt gcagaatcaa
    19141 cacggacaag aacctcggtg tctattttat tgagtgttgc aagtcgatgc acgagagagg
    19201 ttttttcagc ctgaggaagt gcttgaatat aatcggcagc acgtttagga ggcaactggg
    19261 agagtacgag ggatcgaata gcatccgact cgtcatgtaa aagctgctgt attttttcag
    19321 tatccgcttc ttgtagatac tcaaagggtt tcccctttgg aaaggcaatt tttttttgga
    19381 taagcgcttc tgccctctca ggaccaaagg ctttttccaa aatctcatag gcggtttcaa
    19441 ctccaccact ttctcgagat ttttgcacta aactttcaaa ttcagctaga atgagcgctg
    19501 cctcatcctc atcaacacta cgaatagaag ctatttcaag ggcaatttta tcgacttgct
    19561 caggcaaaag atgcggcaaa atttttgctg cctcatcaat gccgattata agtaaaaact
    19621 ttgcaacccg acgaaaaatg ctctcttttt cttcggtttt gcttggaagc ggaaccttta
    19681 ccagcccctg ctttgtaagc tcgtcaagag tctttgtaag agagtctttt gccgattgag
    19741 ctgttttaat caaaggctgt tgaagctcca ccttcgatgt ttgagcagct tctgcatagc
    19801 gcttgagacg ataatcatct tttttcatga ataaattgta gcacaagagc ccttttccgt
//
LOCUS       DAOSSW010000026        19738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_2923_length_19738_cov_6.663720, whole genome shotgun sequence.
ACCESSION   DAOSSW010000026 DAOSSW010000000
VERSION     DAOSSW010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 19738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19738
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2923_length_19738_cov_6.663720"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PKW26_03720"
     CDS             complement(<1..464)
                     /locus_tag="PKW26_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOQ92709.1"
                     /translation="MLEAVLFDMDGLLFDTERLYLKSWKKASEEAGIDFSEDLFFRCV
                     GTNTRDTKKIIFDALGEDFPFDQFEQITSQAVIDEIKANGPPLKKGVHSILAFLKERN
                     IKMALATSTGKEMAKWMVEKAGLELYFDAYAFGSEVKQGKPHPDIFLLAAKNL"
     gene            complement(471..1334)
                     /locus_tag="PKW26_03725"
     CDS             complement(471..1334)
                     /locus_tag="PKW26_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOQ92710.1"
                     /translation="MQSRKSISFNKDENIVSLYLKDINRIPLLSREEETSLALRAADG
                     DKEAKDKIVSANLRFVVSIAKKYQNNGLDLLDLVNEGNIGLLTAIERFDVSKGYHFIS
                     YAVWWIRQAILKAISEKTKMIRLPLNRVNELMQIEKARKELSGGKSEEEELREVAEMV
                     YMKPEQVKELLAISRDLLSLDSTIRFDSSDSTTIGDIVEDGRYESPEEVAVYENMKDE
                     IDEVMQTLTERERDVLAYRFGLNGKPSMSLKEVGDVFNLTKERIRQIEKKAIKRLQHP
                     SRSARLESFVA"
     gene            1646..1912
                     /locus_tag="PKW26_03730"
     CDS             1646..1912
                     /locus_tag="PKW26_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOQ92711.1"
                     /translation="MKVIPLADRVLVKTEKTESKTASGIIIPETAQEKTQIAVVLAVG
                     DDKEKIKVAVGDRVMYDKYAGTQVTIDNEECLILKGQDIIAKIA"
     gene            complement(1914..3665)
                     /locus_tag="PKW26_03735"
     CDS             complement(1914..3665)
                     /locus_tag="PKW26_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ92712.1"
                     /translation="MLKKAFVYGLCIVLFFSCSTKSTNASFIAKLDEADVHIAYSRFS
                     DAEKVLVAAEKMATSDIQYLSVYKRLYKMHKMAACEKVVKKLYKKYGFKPEIQALYTH
                     FLLETKQVAKALSIAKGLVGTDYASLYAEALFLSTNNEKDFFTTDYVQAYIDAYHLTG
                     NTAYSINAACLLAHLGRFREAMFLHPKRMTSYEPAYFWSLIAYDAGDFDTCIFNLSLL
                     AFSEKHLLLEADAYLKAGLLDEANEAWLSVIAHDLKSPVSYLNAARASEEKGLFSEAS
                     QWLLQMLDLFPHFTPGLVEYAYYALRLEKQKSEDILTSNLELRGIKTLQNTKLEDYLQ
                     VPISDALWRIEKSLKEKEDIDLELEQIKLRWAADPQRSRAEKNAEVYALLERNYLGDS
                     LYEERVLQWALWYFLSQRQWKEAEQLFIPYLTTKYGEHSKDAKDEVVIVFDPYNRIAE
                     MRSWEREYLSYFVAEYKKDLMLAYRLLVYEFENGQISTTNEKKDFTQRDRLPLFINLG
                     NMYFGLKQTEKAIELYSLASSQAKSDKQKSEIHYRLALINLHKQEIKNALLNAEYSIL
                     LDSNNTEARLLYKKLKD"
     gene            complement(3669..4082)
                     /gene="nusB"
                     /locus_tag="PKW26_03740"
     CDS             complement(3669..4082)
                     /gene="nusB"
                     /locus_tag="PKW26_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOQ92713.1"
                     /translation="MSRRKARILAFQALYSFDVGKMDSEQLVSLSWVESKSLEKLSES
                     SNLYTRLLIAGTLENLEEIDENISKNLTNWDFERLNRVDLAILRISAYALLYQKDLHP
                     SIVIDEAVQIAKEFGSDDSFKFINAVLDNIQKKEV"
     gene            complement(4099..5178)
                     /locus_tag="PKW26_03745"
     CDS             complement(4099..5178)
                     /locus_tag="PKW26_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ92714.1"
                     /translation="MKKNIGLLIGSLLISASLFAQADLQVLATVKLYKSESITVRQLK
                     ARAEVYKKQTGATNFTLDHKKEILESLIDEKLLLQAAQKDGITVSDSEVNAAFLQSMS
                     EQIGKPLTEPELAQIIKQQTNLSLDDFLKSQTSMNTAEYKQHIKTQIIAQRYLIAKRP
                     EILQISATDAEIRSAYEMNKTNFVQPDVVQLFLVVVAKQNNPDATAEKVGALLKDFKS
                     GKLTQNALREKAKLDPTKMNAGEVYLGKTTSAAQQLGWDFQKLLELFAKPKGFYSEVN
                     ETAGDYQFYQVVEKYDQKTLALSDVVQPGSNYTVYELLKDQLSQQKKSQSLITATTEL
                     ISSLKTPENYQMLKTGEALDTLLTW"
     gene            complement(5246..7054)
                     /locus_tag="PKW26_03750"
     CDS             complement(5246..7054)
                     /locus_tag="PKW26_03750"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOQ92715.1"
                     /translation="MTANELRQKYIAFFKTKNHNEISGKSLLPDNDPTVLFTTAGMQP
                     LVPYLLGETHPQGTRLVNYQKCVRTGDIEEVGDASHLTCFEMLGNWSLGDYFKKESIA
                     YSYEFLTSKDWLNLDPRKLSVTVFAGDENAPRDEESAQYWIEIGMPEDKIAYLGAEDN
                     WWAAGPTGPCGPDTEIFYWVGEGLPKEGSNKETESEHWLEIWNNVFMEYNRVDEKTLV
                     KLPKQNVDTGMGLERTTTILQGKTSVYETEIFEPIIHAIEKEVQEVQNNPNFRYGEAE
                     EKDWSIRIITDHIRSSVFILGDTKGPSPSNLGAGYVLRRLIRRAVRHAKKLGIEKPIL
                     SVLAKTVIETFEKAFPELRENEEKILNELAQEEKRFSETLRKGELEFEKMLPNLMKGN
                     AKLMSGRLAFRLYDTFGFPIELTEELAAEHGLKVNVEEFDAAFKKHQEASKAGSDHLF
                     KGGLAESSEITKRYHTATHLLQKALIQVLGEHVGQKGSNITAERMRFDFTHPAPMTAE
                     EIQQVEAIVNEQIKKALPVTMEVMSLEDAQKSGAIALFGEKYEDKVKVYSVGDFSKEL
                     CGGPHVENTSEIGTFIIKKEQSSSAGVRRIRAVIEN"
     gene            complement(7133..9772)
                     /locus_tag="PKW26_03755"
     CDS             complement(7133..9772)
                     /locus_tag="PKW26_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOQ92716.1"
                     /translation="MAFDKKKVFYFSQTKSVPKTASRDFFGIRARLALDLAEMKLPVL
                     PGLIINSEVADEIEKVDFKKDLESYFTEFSKELGKAFGDENHPLLIKFVISPNMVISQ
                     YPILHNIGLAKDTVEGFADKVGGTFAANEILFLIIGILKVEKMIAESTDDKKYLATVD
                     KTIDSVTKDFKSKEKKQKALSIIEKYEGLLPEGFFESAKKQIEVMLTRVSYLLEVDEQ
                     NDHDTAILIHPMVYGNYTKDSSSGICTTRNVITGEKVIQGMFYNNAYDELFSPGKNIS
                     QFDKKYVSQLTKTAEQLEDTFKDIRQIRFTVENGKLWLIEQKSVEQKSTASLIKLYFD
                     LLKRKIIDDKTLIQSLKPEQLTELLHPVIEEASIKGLDSISGGVSGAPGAAVGRVYFS
                     TEDLLDAKCLAKQQGEDTRCILLMHATYAGDVKAIEEATGVLSNEGGYSAHASVVARQ
                     YGKVSLVKPDMVITGKKAKIGNMVIQSGDYLTIHVPYYGSAVLYKGKASLVQPNPDKL
                     GLFDMVALTKKYVGNFQVRANADSARDAGLSLKFGADGIGLCRTEHMFFQEERINLFR
                     NMVLSQTEEERKKILADLKKIQIKDFYGLLKAMQGKPVTIRLLDAPLHEFLPHNETEK
                     KEYLDYAKNCGIKMTKAALEAQMIALSESNPMLGHRGCRIAISHPEIYEMQVSALFEA
                     ACKLSKEKIKVFPEVMIPIIMNAAELKTLIYGKKIEGYTYTGLAEIAQEVCKKNKVTN
                     LKYSFGTMIELPCAALSSAEIARYAEFFSFGTNDLTQTCLGLSRDDFTSFMPDYTMLD
                     LIDGNPFSVLDSRVKELIELSTKRGTEARPDLSVGLCGEHGARPENIQFCMNGGLHYV
                     SCSPYSVPIALLAVAQENLKKEG"
     gene            complement(9787..10917)
                     /locus_tag="PKW26_03760"
     CDS             complement(9787..10917)
                     /locus_tag="PKW26_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92717.1"
                     /translation="MILHAVQQLETYGNAGVHVADAMNELMYGGDFPEKEILPIIHEL
                     LIKKWSYKARSWNVSVNEIDAKKIYEQVVKWKACDIVIVNHHPDLGLIVLNPKNPQHQ
                     EGLESLKKNELIVVYSGYQGKKESDALCETAVSKTIDALLGKKVTVPDPLLKGSFIYR
                     KPKPELLEKHIEKTASKKAGTGTRAKTAKSAAKAPTRGERVIIGGGAASGSEFSFAQR
                     NATSAGLNTKSIQAKPTSKRTSSITGNAYSGDKNAAKRAQAQTSQPASFRKVTPLVSV
                     PVTNELFHNGNVEAWKRIIASYENKYPNLHIFVYYEGERITDINSLFKWGKVKHGSSI
                     QFVVAGDDIQDVAKLKRYFTQGASPQFEAFLQGAPGTILPLF"
     gene            complement(11015..13006)
                     /gene="cfpA"
                     /locus_tag="PKW26_03765"
     CDS             complement(11015..13006)
                     /gene="cfpA"
                     /locus_tag="PKW26_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014342563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic filament protein CfpA"
                     /protein_id="HOQ92718.1"
                     /translation="MASIDIRKSPNVFDPTVPSAVGSRNSLAQEYRDQQAEVNNLLEE
                     EANKILNHITAKLPEEVLNQLDVMGGLKEKLYNYFNQNYQNMFNRYVVTTEDEMVKKV
                     RNFIDKEEMKVLNRYTPKEIAALLEDVGGADKFNTGEIEKSIVNMYGHLQGHVQRGVN
                     DLETLTNALLRQKSDVGAFVRGENAYSIVKCAFKDNIYKPKTVTDVKLSINILDSELI
                     SPIFHYQVTVEYLIKDLLSKHITDMLDREIEDLKNKRIDDGLDEMSDGELIFAKFNRV
                     TDYTDDDVENQKSKRYSIIAKNLMDKISDLRAEIDPNTFDQLNIRENLKKIIDMENIR
                     NRGFNTAINSLTAILDTSKMGYQYVENLKNARELILREYEDTDAAHLPDERYQVRLKY
                     YDNAQLINEREAYRVQLASFATEVDHLWDVLKTNYDDSKRFKRVADFEDLASRYKRKI
                     ATRYKAKTGDPIYEETAKVWDEISFIKPQETDVEKMNQTFLYEKDKIKSKLIIMRNKM
                     KTRFDYQYPIERRVMEERLAYLEAEFNKFDYMINPFHIQPGLLMDIDITSIKRKKATL
                     DGMANVLNEFLHGISKGFQDAAFASFSRRRSTVRVDLTQSFAQAPSDGHEEKSSAGND
                     LLAELNQDALPPASTAKKAPAKKTAKKGVVDSPKSRRTR"
     gene            13149..13763
                     /locus_tag="PKW26_03770"
     CDS             13149..13763
                     /locus_tag="PKW26_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOQ92719.1"
                     /translation="MKKQSIIAVIFLIFLFSVHGNERKEPIHVFLMIDKSLSMEEDYS
                     FSFLQDWLNKEFLPNNVKKGDSLTVFFFYGETKRVFNKTINNEADLIEIQSIISKEKP
                     NGPFTDIGLAMDSLKEAVQNTKGLPIALIFSDLIQEAAYPSKYAGTYYDFAERYLSED
                     RVFPLKENYYQVTLQLERPETIEKRAKTIYTRIKDSTEEPLYRQ"
     gene            13771..15255
                     /locus_tag="PKW26_03775"
     CDS             13771..15255
                     /locus_tag="PKW26_03775"
                     /inference="COORDINATES: protein motif:HMM:NF033751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pallilysin-related adhesin"
                     /protein_id="HOQ92720.1"
                     /translation="MKQKLSIALFIIAGLALLVIYFTNEYIQSNKKEVSRAQLIIPQT
                     RGESSSQETARISPISEEANITTLLQLEAGETLLAVVTNDIDADTYEDQIVAVKKFNN
                     PFIFILVGLYNPLKMAYERVIEYQTEISQTRTFALSVLDIAGNGQNTIIYTGFTDKNN
                     SFFQALALQKDKGLARLIELIRLESDGSIFLQQNTEKTGDLGYNEPKTTFSILVHSTE
                     TNEEDGSFEQVETSYKWDETTGFFEEFSVEKTPGTRLEAKELAKIQDGTVESFSSYLN
                     GLWLFSESSTTNPYFIFFDWKNKEIIFSSGEIQEIFSWENSTLRRNGIYISTVNSLIA
                     NLNRKIDVSLLSLDEIRIRISSDLGMVINTDTSWDGVYKKTDLQNFKLKKNENSEDNR
                     AIRLLESETNWTFMTSSLLSLSNGNYVFTSGANTEEGVYSEKNVQGESFIQFRSFSKN
                     AFFDGFYTVTKNESSLVLQPVKIHINGSFSYSGSAISLEKKKED"
     gene            complement(15258..15944)
                     /locus_tag="PKW26_03780"
     CDS             complement(15258..15944)
                     /locus_tag="PKW26_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ92721.1"
                     /translation="MKIQSKKVLFFIAVFFIFFSYANLFSYSPEVIRYFSSLKSKEPL
                     ELIESIEKQIGQEEKLNTHPEKTKELYYLLAEVQEKMGDYEEASKNYEKAASQEAFLA
                     AAKTALLYGDSLRCDTLLSRLTQGHIKEKLVSQVRLYAVWSWLVKAQSEDALHEPLVI
                     LKSYVGMKGMEEEQAAILLTLWYLTGEEAYKVAIEKQYPKSLEFLVLESAASFLPTPF
                     WFFVPRKNQG"
     gene            complement(15928..16227)
                     /locus_tag="PKW26_03785"
     CDS             complement(15928..16227)
                     /locus_tag="PKW26_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92722.1"
                     /translation="MPWKLLGFVALLVFATIFIGFNLEHRCDVSIGFTTFKDVPIFLS
                     LLIAFALGVLVMTPFTFIRALSPPQKKGRASEHSKQSQKADTDSAKSKKQHEDTK"
     gene            complement(16242..17639)
                     /gene="miaB"
                     /locus_tag="PKW26_03790"
     CDS             complement(16242..17639)
                     /gene="miaB"
                     /locus_tag="PKW26_03790"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOQ92723.1"
                     /translation="MTYFFETYGCQMNIAESASVERAFIEAGWTKADDVQVADFVIIN
                     TCSVRETAESRILGRLGWYASLKAVRKLEERAKNHVFPKAHSYALTNKENPPRLNLAI
                     MGCMAERLIDSLQKDYPVLDYVIGNFQKQYFPDIIAAVEKGEKWIETDEKPHYVFAES
                     SYEKGSHSAFVPIMHGCNNFCSYCIVPYVRGREVSRSPESIFAEIDALSKNGVKEITF
                     LGQNVNSYIWEEEGKEAIDFPSLMQAVSQHIHATKSSIGWIRFMSSHPKDISPDLIQL
                     VKKDPLFCKHIHLPVQHGSNAILDAMRRKYTREAYLEKIAAIKKEIPGVSLSTDIMIG
                     FPGETIEDFEETIALMREVAFENAFMYYYNPREGTKAADMQEQLSIKEKKRRLALVIA
                     EQQAISKRLMEERLGESVRVLAEGFSKDNPEELVGRTERDERVVFPAGPEKIGHFVEV
                     FLTELTGATLRGKIV"
     gene            complement(17636..18466)
                     /gene="folE2"
                     /locus_tag="PKW26_03795"
     CDS             complement(17636..18466)
                     /gene="folE2"
                     /locus_tag="PKW26_03795"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOQ92724.1"
                     /translation="MLENKTEKNSKSHSLIDMQNEVDERDIPLQKVGVRGLQYPVFVL
                     DKNHKQQSTTASIDLFVNLPHHFKGTHMSRFIEIVHSYHKDLSMKQFLAMLSEVREKL
                     EAQKAFGSLRFPFFLEKQAPISKQSGMMSYACSYEGIVSKNEKEFYVGIEVPVTTLCP
                     CSKAISKYGAHNQRGVVRVRLRNEGFFWIEDVISMIEACASSGVYSVLKREDEKYVTE
                     KAYENPRFVEDLVREVYQGLVAFESEKKFSWFSVEAENYESIHFHNAYAYTEFLGDRE
                     "
     gene            complement(18453..19334)
                     /locus_tag="PKW26_03800"
     CDS             complement(18453..19334)
                     /locus_tag="PKW26_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HOQ92725.1"
                     /translation="MAAIIIDGFEIASQVRADVAKKVLALKAKQIEACLAVVLVGEDP
                     ASISYVTGKEKALQEVGMKDRSIRLPATTSQEELLALIDELNKDESVHGILVQLPLPK
                     HLEEAAVIQAIDPKKDVDGFHPVNVGNMLIGNKSFLPCTPHGVLVLLEKMGISTKGAH
                     VVIVGRSNIVGKPLAVLLSRRESNATVTLCHTGTKDLASFTRQADILIAAVGRANTIT
                     ADMIKKDAVIIDVGVNRIPDESKKSGFRLVGDVAFDEVKEKAAFITPVPRGVGPMTIA
                     MLMWNTLEAAENADARK"
     gene            19475..>19738
                     /locus_tag="PKW26_03805"
     CDS             19475..>19738
                     /locus_tag="PKW26_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92726.1"
                     /translation="MKKYLSLCFCLCFLSLAVFAQMNEDEQDKDAKTEELYVYKMNQK
                     GDWFIKLGGAAFIPLQPKNMNLGLDLNLGFYRFLWSEFSLGGDI"
BASE COUNT         5854 a         4009 c         3431 g         6444 t
ORIGIN      
        1 aggttttttg cggcaagaag aaagatatcg ggatggggtt ttccttgctt tacctcggag
       61 ccaaaggcat aggcatcgaa atagagctct aaaccagctt tttctaccat ccatttcgcc
      121 atctcttttc ccgtagaagt cgcaagcgcc attttaatat ttcgctcttt taaaaaagcc
      181 aaaatactat gcacaccttt tttaagcggc ggtccatttg ccttaatctc atctataaca
      241 gcttgcgagg ttatttgttc aaattgatcg aagggaaaat cctctccaag cgcatcgaag
      301 atgatttttt tagtatccct tgtatttgtt cccacacagc gaaaaaacaa atcttcagaa
      361 aaatctatcc ctgcttcttc gctcgccttt ttccaagact tcaaatacaa tctttcggta
      421 tcgaacaaaa ggccatccat gtcaaataat acagcttcta acataggcat ctaggcgaca
      481 aaggattcta aacgagctga ccgactcgga tgctgcaaac gcttaattgc ttttttttca
      541 atttgtcgaa tgcgctcttt tgtcaaatta aaaacatcgc ccacttcttt taaagacata
      601 ctcggttttc catttaagcc aaagcgatag gcaagcacat cccgttcacg ttcggtcagg
      661 gtttgcatca cttcatctat ttcatccttc atattttcat acaccgcaac ttcttcaggg
      721 ctttcatagc gcccgtcttc aacaatatcg ccaatcgtag tcgaatcaga actatcaaat
      781 cgaatagtgg agtcgagaga gagcaaatct ctagaaatag ccagaagttc ttttacctgc
      841 tcaggtttca tgtacaccat ctctgctact tctctaagct cttcttcttc cgattttcca
      901 ccgctcaact ccttccgagc cttttcaatt tgcatcaatt cattgactcg attcaaaggc
      961 aagcgaatca tctttgtttt ttcgcttatc gcttttaaaa ttgcttggcg aatccaccaa
     1021 accgcataag aaataaagtg atatcccttg gaaacatcaa aacgctcaat cgcagtcaac
     1081 aagccgatat tcccctcgtt tacaaggtca agcaagtcca agccattgtt ttgatatttt
     1141 tttgcaatcg ataccacaaa acgcaaattt gcacttacta ttttatcctt tgcttcctta
     1201 tcgccatcgg ctgcacgaag cgcaagactc gtttcttctt cccttgacaa caagggtatt
     1261 ctgtttatat cttttaagta gagagagaca atattttcgt ccttattaaa tgatattgat
     1321 tttctgcttt gcatatgtcc tcctaaaatg tatctcgtat attagttata ataatgcaag
     1381 tttcatgcca actttttatt tttggaaatc aaataaaaaa atgtaattcc ttgtattgta
     1441 aagaattaga aaaatcaata tattttctta aataagagct ttttcttctg tgtcatttta
     1501 acacaatatc cttaaatttc aaaaaaactg cgtcaataca gtacattttt tttaatatat
     1561 ctttatttat ttttttcctt gccctttttt catatatttt gtatctttga agtatcaaaa
     1621 tacaataaag gagctttgca caaatatgaa ggttatccct ttagcagacc gagtcttggt
     1681 taaaacagaa aaaactgaat caaaaaccgc tagtggtatt attatcccgg agacagcaca
     1741 agaaaaaaca caaattgctg tggtgcttgc tgtcggcgac gataaagaaa aaatcaaggt
     1801 tgctgtagga gaccgcgtaa tgtacgataa gtatgcggga acacaggtaa caatcgataa
     1861 tgaagaatgc cttatcttaa aaggccaaga cattatagca aaaattgctt aaactaatct
     1921 tttagttttt tgtacaaaag acgtgcttca gtattgtttg aatccagcaa aatgctgtat
     1981 tcggcattca gaagcgcgtt ttttatttcc tgtttatgca agtttataag cgcgagtcta
     2041 tagtgtattt ccgacttttg tttatcagat tttgcttgac ttgaagcgag agaataaagc
     2101 tctatcgcct tttcagtttg cttaagacca aaatacatat ttcccaagtt tataaaaagg
     2161 ggaagtctat ctctctgagt aaaatctttt ttttcgtttg ttgtggaaat ttgtccgttt
     2221 tcaaactcat agacaagcaa tcgatatgca agcatgagat cttttttata ttcagccaca
     2281 aaataagaca aatactctcg ttcccaggaa cgcatttctg ctatccgatt gtaaggatca
     2341 aaaacaataa caacctcatc ttttgcatct tttgaatgct caccatattt tgttgttaaa
     2401 taggggataa acagttgttc cgcttccttc cattgccttt gcgataaaaa ataccaaagt
     2461 gcccattgca aaactctttc ctcgtataaa gaatccccca aatagtttct ttctagtagc
     2521 gcatagactt cagcattttt ttctgcccga cttcgttgtg ggtctgctgc ccagcgcaat
     2581 ttaatttgct caagctctag atcaatgtct tctttttctt ttagagattt ttctattcgc
     2641 caaagtgcat cgctaatggg aacttgtagg tagtcttcga gtttcgtgtt ttgcagtgtt
     2701 tttatcccgc gcagctctaa gttagaagtc aatatatctt ctgatttttg tttttctaaa
     2761 cgaagtgcat aataggcata ttcaactaaa ccaggcgtaa aatgcggaaa aagatccagc
     2821 atttgtaaaa gccattgcga tgcttctgaa aaaagcccct tttcttcgct tgcacgggca
     2881 gcattcaaat acgaaacagg cgattttaaa tcatgggcaa taacagaaag ccaggcttca
     2941 tttgcttcat cgagtaagcc agcttttaga taggcatcag cttcaagtaa gagatgtttt
     3001 tcagagaagg ctaaaagaga aagattaaaa atacaggtat caaaatcgcc cgcgtcataa
     3061 gctatgagcg accaaaaata agcaggctca tagcttgtca tgcgttttgg atgaaggaac
     3121 attgcttctc gaaatcgtcc gagatgtgca agcaaacatg cagcatttat actataggct
     3181 gtattgcctg tcaaatgata ggcatcgata tatgcttgga cataatccgt cgtaaaaaaa
     3241 tctttttcat tatttgtcga taaaaaaaga gcctcggcat acaaggaagc ataatctgta
     3301 ccgaccaagc ctttcgctat cgatagggct tttgcgactt gttttgtttc aagcaaaaaa
     3361 tgtgtatata gggcctgtat ttcaggctta aaaccatatt ttttatacag cttttttact
     3421 accttttcac aggcagccat cttatgcatt ttataaaggc gtttatacac ggaaagatac
     3481 tgaatatcgc tcgtcgccat tttctcggca gcaacaagaa ccttttctgc gtcagaaaaa
     3541 cgagagtacg caatatgcac atcagcttca tcaagttttg ctataaaaga agcatttgtc
     3601 gatttggttg agcatgaaaa aaagaggact atgcacaatc catacacaaa ggcttttttt
     3661 aacatagact atacttcttt tttttgaata ttgtccaaaa cagcatttat aaacttaaag
     3721 gaatcgtcag agccaaactc ttttgcaatt tgcaccgcct catctattac gatagaagga
     3781 tgcaaatctt tttgatataa caaggcatag gcagatatgc gtaaaatggc caaatcaact
     3841 cgattcaaac gctcaaaatc ccagtttgtt agattttttg aaatattctc atctatctct
     3901 tcaagatttt ctagagttcc agctattaaa agcctagtat aaagattgct tgactcactg
     3961 agtttttcga ggcttttact ttctacccat gaaagagaaa ccaattgttc tgaatccatc
     4021 ttgcccacat caaacgaata aagcgcttgg aaggctagga ttcgagcttt tctacgagac
     4081 ataaaaaact cccgtatttt accaagttaa aagagtatct aatgcttcac ctgtttttaa
     4141 catttgataa ttttctggtg ttttaagtga cgaaatcaat tccgttgttg cggtaatcaa
     4201 agactgtgat tttttttgtt ggctcaactg gtccttaagt aactcatata ccgtataatt
     4261 cgaacctggt tgtacaacat cactcaaagc caaggttttt tgatcatatt tttcaacgac
     4321 ttgataaaac tgataatcac ctgctgtttc attcacttca gagtaaaatc ccttcggttt
     4381 tgcaaacaat tcgagcagtt tttgaaagtc ccaacctaac tgctgagctg cagaagttgt
     4441 tttccctagg tacacttcac cagcattcat ctttgtggga tcaagctttg ctttttcacg
     4501 aagagcattt tgcgttaatt tacctgactt aaaatctttc aaaagtgcgc ctaccttttc
     4561 agccgttgca tccgggttat tttgtttggc taccacaacc aaaaaaagtt gcacgacatc
     4621 gggctgtaca aaatttgttt tattcatttc atatgcagag cgaatttcgg catctgttgc
     4681 agaaatctgt aaaatctccg gtctttttgc aatcaaatag cgttgcgcaa taatttgcgt
     4741 tttaatatgt tgtttatatt cagcagtatt catcgaggtt tgtgatttta agaaatcatc
     4801 taaactcaaa tttgtttgtt gcttaattat ttgtgccaat tcaggttcag ttaaaggttt
     4861 cccaatctgc tctgacattg attgtaaaaa agctgcattt acctctgaat ccgatacagt
     4921 aataccgtct ttttgcgcgg cttgaagtaa aagtttttca tcgattaaag attctaaaat
     4981 ttctttttta tggtctaggg taaaattggt agcaccagtt tgttttttat acacttctgc
     5041 gcgcgcttta agttgacgca ctgtaattga ttcgcttttg tacaatttta ccgttgcaag
     5101 cacttgcaaa tctgcctgtg caaaaagact cgcacttatc aaaagtgacc ctatcaataa
     5161 tccaatattc tttttcattt tttcacctta taaatcaata tataataaaa ccgtttagtt
     5221 tagttttagt tacagggata taaaattagt tttctataac agctcgaatt cggcgaacac
     5281 ccgcagaaga ggactgttct ttttttataa taaaagtacc aatttcactc gtattctcga
     5341 catgcggtcc accgcataat tcttttgaaa aatcacccac actgtacacc tttaccttat
     5401 cttcgtattt ttcaccaaat aaggcaatag cgccagattt ttgtgcatct tcgagagaca
     5461 ttacttccat cgtaacaggc aaggcttttt ttatttgctc atttacgata gcttcaactt
     5521 gctggatttc ctcagctgtc atgggggcag gatgtgtgaa gtcaaaacgc atacgttcag
     5581 ctgtaatgtt tgagcctttt tgcccaacat gctctcccaa aacctgtatc aaagcttttt
     5641 gtaaaaggtg ggttgcggta tgatagcgct ttgtaatctc tgagctttct gccaagccac
     5701 ctttgaacag atgatcgctt cctgccttcg atgcctcttg gtgtttttta aatgctgcat
     5761 caaattcttc gacattcacc tttaaaccat gttcagcagc aagctcttct gttagttcaa
     5821 tgggaaagcc aaaagtatcg tagagcctaa acgcaagtct cccagacatt agctttgcat
     5881 tccccttcat aagatttggt agcatttttt caaactctag ctcgcctttt cttagcgttt
     5941 cgctaaatcg tttttcttct tgggcgagtt catttagaat tttctcttca ttttctcgca
     6001 attctggaaa agctttttca aaggtttcta taacagtctt cgcaagcaca gaaagaatgg
     6061 gtttttctat gccaagtttt tttgcatggc gaacagctct tcggattaaa cgtcgtaaaa
     6121 catagcctgc tccaaggttt gaaggacttg ggccctttgt atctccaaga ataaaaacac
     6181 tcgatcgaat atgatcggtg atgatgcgaa tgctccaatc tttttcttca gcttctccat
     6241 accgaaagtt cggattattt tgcacttctt gcacttcttt ttctatagca tgaattatag
     6301 gttcaaagat ttctgtttca tatacggacg tttttccctg caaaattgtc gtagtgcgtt
     6361 ctaagcccat accggtgtca acattttgtt ttggtagctt tacgagtgtt ttttcatcaa
     6421 ctcgattata ttccataaaa acattgttcc agatttcaag ccagtgttcg ctttcggttt
     6481 ctttatttga accttctttt ggaagtcctt ctcctaccca ataaaaaatc tcagtatcag
     6541 gaccacaagg gccagtagga ccagctgccc accaattatc ttcagcgcca agataggcta
     6601 ttttatcctc aggcattccg atttctatcc aatactgagc ggattcttca tctcgcggtg
     6661 cattttcatc accagcaaaa acagttacag agagttttcg tgggtcaagg tttaaccagt
     6721 cttttgaagt taaaaactca taactatagg ctatggattc ttttttaaaa taatcaccga
     6781 gcgaccagtt acccaacatc tcaaagcagg taagatggct cgcatccccc acttcttcga
     6841 tatcgccggt tcgaacgcat ttttgataat tgacgaggcg tgttccttgt ggatgcgttt
     6901 caccaagcaa ataaggtaca agaggttgca ttcctgcggt cgtaaacaag accgttgggt
     6961 cattgtctgg caagagagat tttccagata tttcattgtg attttttgtt ttaaaaaagg
     7021 caatgtattt ttgtcgtaat tcgtttgctg tcatactctt tatcttagat atggacgccc
     7081 tcaatgtcaa gggtagaaag acaagggttt tctaccccaa acaaaaagag ttttatcctt
     7141 ctttttttaa attctcttgt gcaactgcta aaagcgcaat tggtactgaa tatggtgaac
     7201 aagaaacata gtgtaaaccg ccattcatac aaaactgtat gttttcaggt cttgcaccat
     7261 gctcgccaca aagacccaca gaaagatcag gacgtgcttc tgtaccacgc tttgttgaaa
     7321 gctcaatcaa ttcttttaca cgtgaatcta aaactgaaaa aggatttccg tcgatgagat
     7381 cgagcatagt ataatcaggc ataaaactcg taaaatcatc gcgagaaagt cctagacagg
     7441 tttgcgtcaa atcatttgtt ccaaaggaga aaaattctgc gtaacgagct atttctgcag
     7501 aactgagagc cgcacaagga agttcaatca ttgtaccaaa gctatatttt aggttcgtta
     7561 ccttattttt tttacatact tcttgagcta tctcagccaa tcctgtataa gtatagcctt
     7621 caattttttt cccatatatg agcgttttca actccgctgc gttcataata atcggaatca
     7681 taacctcagg aaataccttt atcttttctt tgcttaactt acatgcagct tcaaagagtg
     7741 ccgaaacttg catttcataa atttctggat gtgatatagc tattcgacaa cctcggtgac
     7801 caagcatcgg attgctttct gaaagagcaa tcatttgcgc ttctagagct gcctttgtca
     7861 tttttatgcc acagtttttt gcatagtcta gatactcttt tttttcagtt tcattgtgag
     7921 gcaaaaattc atgtagagga gcatcgagca aacgaatagt tacaggcttc ccttgcattg
     7981 cttttagaag tccataaaaa tcttttatct gtatcttctt taagtccgcg agaatttttt
     8041 ttctctcttc ttcagtttga gataaaacca tatttctaaa aagattaatg cgttcttctt
     8101 ggaagaacat atgttctgtt cgacaaagtc caataccatc tgccccaaac tttaaggaaa
     8161 gtcctgcatc tcgggcggag tcagcatttg cgcgtacttg aaagtttcct acgtattttt
     8221 ttgtcaaagc aaccatgtca aataagccaa gtttatcagg gtttggttgc acaagagaag
     8281 ccttgccctt atataaaacg gcagaaccat aatagggaac atgaatagtg agataatcac
     8341 cggactgtat caccatattg cctatcttcg cttttttccc tgttataacc atgtcgggtt
     8401 ttacaagtga gaccttcccg tattgacgag caacaacaga agcgtgagcc gaataccctc
     8461 cttcattaga aagcacccca gttgcttctt cgattgcctt aacatcacct gcataggttg
     8521 catgcatgag caaaatacaa cgagtatctt ctccttgctg ttttgcaagg cactttgcat
     8581 cgagcaaatc ttcagtagaa aaatacacgc gcccaactgc tgcaccagga gcacctgaaa
     8641 caccaccgct tatggagtca agacctttta tagaagcttc ttctataacg ggatgcagga
     8701 gctcagtcaa ttgctctggt tttaaacttt gtataagcgt tttatcgtcg atgatttttc
     8761 gtttcaacag gtcaaaataa agcttaatca aagatgcggt acttttttgc tcaacagatt
     8821 tttgttctat aagccaaagt tttccatttt ctactgtaaa gcgaatttgg cgaatatctt
     8881 taaaagtatc ttcaagttgt tctgccgttt tcgttaactg acttacatat tttttatcaa
     8941 actgcgaaat atttttccca ggggaaaaaa gctcatcata ggcattatta taaaacatac
     9001 cttggattac tttttcgcca gtaataacat tccgagtagt acaaattcct gagctagaat
     9061 ccttcgtata gtttccatat accataggat ggataagtat tgctgtatcg tggtcatttt
     9121 gctcatcaac ttctaataaa taactaacac gagtaagcat cacttcaatt tgtttttttg
     9181 ccgattcaaa gaaaccttca ggcaagagcc cctcatattt ttcaataata gagagcgcct
     9241 tttgtttttt ttccttcgac ttaaagtcct ttgtcacaga atcgattgtt ttatccactg
     9301 ttgcaagata ttttttatca tccgtacttt ctgcaatcat tttttcgact ttcaaaattc
     9361 cgataattaa aaataagatt tcattcgcgg caaaagttcc accaaccttg tcggcaaagc
     9421 cttcaacagt atctttcgca agcccaatat tatgtaaaat aggatactga cttataacca
     9481 tatttggaga tataacaaat tttataagca aagggtgatt ctcgtcacca aatgctttcc
     9541 ccagttcttt agaaaactct gtaaaatagc tttctaaatc ttttttaaaa tcaacttttt
     9601 ctatttcgtc tgcaacttca ctgttaataa ttaagccagg taaaacgggc agtttcattt
     9661 ctgccaaatc caaggcgaga cgagccctga tgccaaaaaa atcacgagag gctgtcttgg
     9721 gaacagactt tgtttgagaa aagtaaaaaa cctttttttt atcaaatgcc ataaaacaac
     9781 tccttcttag aacaatggta aaatagtacc gggtgcacct tgcaaaaatg cttcaaactg
     9841 agggctggcg ccttgtgtaa agtagcgctt tagcttggca acatcttgaa tgtcatctcc
     9901 agctacaaca aattgtatac tacttccgtg ttttacctta ccccacttaa ataaagaatt
     9961 aatatcggtt atacgttcac cttcataata cacaaaaata tgtaggtttg ggtatttgtt
    10021 ttcataactt gcaataatcc ttttccaagc ttcaacgttt ccattgtgaa ataattcatt
    10081 cgtaacagga acagatacaa gtggcgtcac ttttctaaaa ctcgcaggct gactcgtttg
    10141 tgcttgagcg cgttttgctg catttttatc acctgagtac gcatttccgg taatcgaaga
    10201 agtccttttc gaagtgggtt ttgcttgaat agacttggtg ttaagaccag cacttgtagc
    10261 atttctttgg gcaaaagaaa attcacttcc acttgcagct cctccaccga ttataactcg
    10321 ctctcctctc gtaggtgctt ttgcagcaga ttttgccgtt tttgcccttg ttccagtgcc
    10381 agccttcttt gaagcggttt tctctatatg tttttccagt agttctggtt ttggttttct
    10441 atatataaaa gaacccttta acaaggggtc gggaacagtc actttttttc caagcagggc
    10501 atcaattgtt ttgcttaccg ctgtttcgca aagtgcatca ctctcttttt taccctgata
    10561 accagaatac acaacaatga gttcattttt tttaaggctt tctaaaccct cttggtgttg
    10621 tggatttttg ggatttaaaa caatgaggcc taaatccgga tgatgattta cgattacaat
    10681 gtcacaagcc ttccacttga caacttgctc gtatattttt tttgcgtcta tttcatttac
    10741 cgaaacattc cacgagcgag ccttgtatga ccattttttt attagcaact cgtgaataat
    10801 aggtaaaatt tctttttcag gaaaatcgcc cccatacatt aattcgttca ttgcatcagc
    10861 aacatgaact ccagcgtttc cataagtctc aagttgttgc actgcatgta aaatcatttt
    10921 tgtctcctct taattaaata tatcctataa aaaaaggtgc gttataattg ataaacaact
    10981 acacgcacct ttagtatagc acaagcttta taatttagcg agtacgtctt gattttggtg
    11041 agtcaacaac tccctttttt gctgtttttt tagcaggagc tttcttcgca gtacttgctg
    11101 gtggcaaagc atcttgattt aactcagcaa gtaagtcatt gcctgctgaa gatttctctt
    11161 catgcccatc tgaaggagct tgagcaaaag actgtgttaa atcaacacga acggtagatc
    11221 ttcttcgtga gaaagaagca aaggcagcat cttggaagcc ctttgaaata ccatggagga
    11281 attcattaag cacatttgcc ataccatcga gcgttgcctt ctttcgtttg atagaagtaa
    11341 tatcgatatc catcaacaaa cctggttgaa tatggaaggg gttaatcatg taatcaaact
    11401 tattgaattc tgcttcaaga tatgcaagtc tttcttccat aacccgtctt tcaattggat
    11461 actgataatc aaagcgcgtt ttcatcttgt ttctcataat aataagcttg ctcttaatct
    11521 tatctttttc atataagaaa gtttgattca ttttttcaac atcagtttct tgtggtttta
    11581 taaaggaaat ttcatcccat acttttgctg tttcttcata aataggatcg ccagtttttg
    11641 ccttataacg agttgcaatt tttctcttat agcgactagc aaggtcttca aagtcagcta
    11701 cacgcttaaa tcgttttgaa tcatcgtagt tcgtttttaa aacatcccaa aggtgatcga
    11761 cttcagttgc aaaactagct aattgaactc gatacgcctc tctttcattg ataagctgtg
    11821 cattgtcata gtacttcaag cgcacttgat atctttcatc tggaagatga gctgcatcgg
    11881 tatcttcgta ttcgcgaaga attaattcac gagcattctt taagttttct acatattggt
    11941 agcccatttt tgaagtatca agaatagcag ttaaagagtt gattgcggta ttaaaaccac
    12001 ggttacgaat gttttccata tcgatgattt tcttaagatt ttcacgaatg ttaagttgat
    12061 cgaaggtatt tggatctatt tctgcacgta aatcagaaat tttgtccatt aaattttttg
    12121 caataatact gtatcgttta ctcttttggt tttcgacatc atcatcagta taatcagtta
    12181 cacgattaaa ctttgcaaaa ataagttcgc catctgacat ttcatctaaa ccatcgtcaa
    12241 ttcttttatt ttttaagtct tcaatttcac gatctaacat gtcagtaata tgttttgaaa
    12301 gaagatcttt aatcaaatat tctaccgtta cttgataatg aaaaatagga ctgataagtt
    12361 ctgaatcaag aatgttaata gaaagtttta catcggtaac tgtttttggt ttataaatat
    12421 tgtctttgaa tgcacactta acgattgaat atgcattttc accacgaaca aaggcaccaa
    12481 catcagattt ttgtcgaagc aaggcgtttg ttaaggtttc aagatcattt actccacgtt
    12541 gtacgtgacc ttgcaagtgt ccatacatat ttacaatgga tttttcaatt tcacctgtgt
    12601 taaacttatc tgctccacca acatcttcaa gtaaggcagc aatttctttt ggcgtatagc
    12661 ggttgagaac cttcatttct tctttgtcga tgaagtttct aaccttttta accatttcat
    12721 cttcagttgt aacaacataa cggttaaaca tgttttgata gttttgatta aaatagttat
    12781 ataacttttc tttcaaacca cccataacat caagctggtt taacacctct tctggtaatt
    12841 tcgcagtaat atgatttaaa attttgttcg cttcttcttc aagcaaattg ttcacttctg
    12901 cttgttgatc tctatactct tgagcgagcg agtttctaga acctacggca cttggcactg
    12961 taggatcaaa tacgtttggg ctttttcgaa tatcaatact tgccatattt tctccttagg
    13021 attattttcc tctctgttac tctacaccta taattataag caaagtttta agaatgtaaa
    13081 gcattttttt ttatattaaa aaaaagatat gtatgttata ctaaaattat agtttttagg
    13141 gggtttttat gaaaaaacaa agcatcatcg ctgtaatttt tcttattttc ttattctcag
    13201 tacatggaaa tgaacgaaaa gagccaatcc atgttttttt aatgatcgat aagtctcttt
    13261 ctatggaaga agactatagt ttttcttttt tgcaggactg gttgaacaaa gaatttcttc
    13321 caaacaatgt aaaaaaaggg gatagtctca ctgttttttt cttttatgga gaaacaaaaa
    13381 gagtttttaa taaaacaatt aacaatgaag cagatttaat tgaaattcaa agtattattt
    13441 ctaaagaaaa accaaacggt ccctttactg atataggtct ggctatggat agcttaaaag
    13501 aggctgttca aaatacaaaa gggcttccca ttgcgcttat tttttcagat ctcatacagg
    13561 aagctgccta tccttcaaaa tacgcaggaa cctattacga ctttgcagaa cgttatctca
    13621 gtgaagatag agtttttcct cttaaagaga actattatca agtaacgctt cagttagaaa
    13681 ggcctgaaac tatagaaaag agagcaaaaa ctatttatac tcgtataaaa gattcaacag
    13741 aggagccctt gtaccggcaa taagcaaaac atgaaacaaa aactaagtat agctctattt
    13801 atcatcgcag gccttgcctt gctcgtgatt tattttacaa atgaatatat tcaatcaaat
    13861 aaaaaggaag taagccgagc gcagctcatt attccgcaaa caaggggtga aagcagtagt
    13921 caagaaacag cgcgcatttc acccatatcc gaagaagcaa atataacaac gcttctacaa
    13981 ctcgaagcag gtgaaacgct tttggcagtt gtgactaatg atatcgatgc tgatacttat
    14041 gaagatcaaa ttgttgcggt aaaaaaattt aataacccct ttatatttat ccttgttggt
    14101 ttatataatc ctctaaaaat ggcttatgag cgcgtaatag aatatcaaac agagatttcc
    14161 caaacaagaa cctttgcact ttcagttttg gacattgcag gaaatgggca aaacaccatt
    14221 atctataccg ggtttacaga caagaacaat tctttttttc aagcacttgc cttacaaaaa
    14281 gacaaaggac ttgctcggct catagaactt atacgcttag aatcggatgg atctattttt
    14341 ctccagcaaa ataccgaaaa aacaggtgat ttaggttata acgaacctaa aacgactttt
    14401 tcaattcttg tacatagcac cgaaacaaat gaagaagacg gttcttttga acaagtagaa
    14461 acaagctata aatgggatga aacaacagga ttttttgaag aattttctgt ggaaaaaaca
    14521 cccgggacac gacttgaagc aaaggaactc gcaaaaattc aagatggaac ggtcgaaagt
    14581 ttttcctcct atctcaatgg actttggctt tttagtgaaa gtagtacaac aaacccctat
    14641 tttatatttt ttgactggaa aaataaggaa attatttttt catcgggaga aattcaagaa
    14701 attttttcct gggaaaatag tactctgcga cgaaatggaa tatatatttc cacggtaaac
    14761 agtctcatcg caaacttaaa cagaaaaata gatgtaagct tactttctct tgatgaaata
    14821 cgcattcgca ttagttctga cctaggaatg gtaataaata ctgacacaag ttgggatggg
    14881 gtctataaaa aaacagactt gcaaaatttc aagctcaaga aaaacgaaaa ctcagaagac
    14941 aatagggcaa tacgtttact tgaaagtgaa acaaactgga cttttatgac aagcagcctt
    15001 ctaagccttt caaatggaaa ctatgttttt acttctggag cgaatacaga agaaggtgtc
    15061 tattctgaaa aaaacgtgca aggtgaaagt tttatacaat ttcgctcatt ttctaaaaat
    15121 gcctttttcg atggttttta taccgtcaca aaaaacgaaa gctcgcttgt attgcaacca
    15181 gtaaaaatac atattaacgg aagtttttcg tattctggct ccgcaattag tttagaaaaa
    15241 aagaaggaag attagcatta gccttgattt tttctcggaa caaaaaacca aaagggtgtt
    15301 ggcaaaaaag atgcagcgct ttctaaaaca agaaactcaa gagatttagg gtactgtttt
    15361 tcaattgcga ctttgtatgc ttcttctccg gtaagatacc aaagcgttaa aagaatcgcc
    15421 gcttgctctt cttccattcc cttcataccg acataggatt ttaagataac gaggggctca
    15481 tgcagggcat cttcgctctg cgctttaaca agccagctcc acactgcata aaggcgaacc
    15541 tgagatacaa gcttttcttt tatatgtcct tgtgtaagcc gagataaaag agtatcgcag
    15601 cgaagactat caccgtacaa gagggctgtt tttgccgcag ctaaaaaagc ctcttgtgaa
    15661 gcagcttttt catagttttt tgaagcttcc tcgtagtcac ccattttttc ttgcacttcc
    15721 gcaagtaaat aataaagctc tttcgttttt tcaggatggg tattgagttt ttcttcttgc
    15781 ccaatctgtt tttctatact ttctataagc tcaagaggtt ctttgctttt aagactggaa
    15841 aaatatcgta taacttcggg agaatatgaa aaaaggttcg catatgagaa aaaaataaaa
    15901 aaaactgcta taaaaaaaag gactttttta ctttgtatct tcatgttgtt ttttagactt
    15961 tgcactatct gtatctgcct tttgagactg cttactatgc tctgatgccc ttcctttttt
    16021 ctgaggtggg ctaagtgctc gtataaaggt aaagggagtc ataactaaga caccgagggc
    16081 aaaggcgatg agtaagctca aaaaaatagg cacgtcttta aaagtagtaa agccaataga
    16141 aacgtcacat ctatgctcta aattaaaacc aataaaaatc gttgcaaata ccaataaggc
    16201 tacaaagccc aacaacttcc aaggcataca atacctccct tttagacaat tttaccacgg
    16261 agcgtagcac ccgtcaattc tgttaaaaaa acttctacaa aatgccctat tttttctgga
    16321 cctgcaggaa acacaactcg ttcgtcgcgc tccgtccgtc ccacaagctc ttcaggatta
    16381 tcttttgaaa agccctctgc caaaacccgc acgctttctc caagtcgctc ttccatgagc
    16441 cgttttgata tagcttgttg ctcagcaata accaaggcaa ggcgacgttt tttctctttt
    16501 atagacaatt gctcctgcat gtcggcagcc tttgtcccct ctcgtggatt ataataatac
    16561 ataaacgcgt tttcaaaggc tacttcacgc atcaaagcga tggtttcttc aaaatcttct
    16621 atagtttcac ctggaaaccc tatcattata tcggtagaaa gtgaaacccc aggtatttct
    16681 ttttttatag cggcaatttt ttcaagatag gcttccctcg tatactttcg tctcatcgca
    16741 tcgagaatgg cgtttgagcc atgttgcaca ggcagatgaa tatgcttaca aaaaagtggg
    16801 tcctttttaa ctagctggat taggtcaggg cttatatctt ttggatggct cgacataaaa
    16861 cgaatccaac caatgctcga ctttgtagca tgaatatgtt gagaaacagc ttgcattaaa
    16921 gagggaaaat ctatcgcctc ttttccttct tcttcccata tgtaggagtt tacgttttgt
    16981 cccaaaaagg taatttcttt tacgccattt ttagacagtg catctatttc tgcaaaaata
    17041 ctttctggac ttcttgaaac ttccctaccg cgaacatagg gaacaatgca ataagaacaa
    17101 aaattattgc aaccgtgcat gatgggaaca aaggctgagt gagagccctt ttcatagctg
    17161 gactctgcaa aaacataatg aggtttttcg tcggtttcta tccatttttc gcctttttct
    17221 accgctgcaa taatgtcggg gaagtattgt ttttgaaaat ttcctataac atagtctaaa
    17281 acaggatagt ctttttgcaa gctgtctata agccgttctg ccatacatcc cataattgca
    17341 agattgaggc gaggcggatt ctctttattt gtcaaggcat agctatgtgc cttgggaaaa
    17401 acatggtttt ttgccctttc ctcgagtttg cgaacagcct taagagatgc ataccagccg
    17461 agtctgccca aaatacgact ttctgctgtc tcgcgtacgg aacaagtgtt aataataaca
    17521 aagtctgcaa cttgcacgtc gtcagccttt gtccaaccag cttctataaa tgctctttca
    17581 accgaagctg actctgctat attcatttga cagccatagg tttcaaaaaa ataggtcatt
    17641 ctctatctcc caaaaactcg gtataggcat aggcattgtg aaagtgaata ctctcgtagt
    17701 tttctgcttc cacgctaaac cagctaaact ttttttcact ttcaaaagcc acgagaccct
    17761 gatatacctc tcgaacgaga tcttctacaa aacggggatt ttcataggct ttttctgtca
    17821 catatttttc atcttcgcgt ttaagaacag aataaactcc agaagaagcg caggcttcta
    17881 tcatgcttat aacgtcttct atccaaaaaa aaccttcatt tctaagccga acacgtacaa
    17941 ctccacgctg attatgagcg ccgtattttg aaatagcctt tgaacaggga cagagggttg
    18001 ttactggcac ctcgattcca acataaaatt ccttttcgtt ttttgaaaca attccctcat
    18061 aactacaagc atagctcatc ataccgcttt gcttagaaat aggagcttgt ttttctaaaa
    18121 aaaaaggaaa gcgtaaacta ccaaaggctt tttgtgcttc aagtttttcc cgcacctcag
    18181 aaagcatagc taaaaactgc ttcatcgaaa ggtctttatg atagctatgg acaatttcta
    18241 taaaacggct catatgagta cctttaaagt gatgcggtaa gtttacaaat aaatcgatgc
    18301 ttgctgttgt cgattgttgt ttgtggtttt tatccaaaac aaaaacagga tactgcaggc
    18361 cccgaacacc aaccttttgc aaaggaatat cacgctcgtc aacttcgttt tgcatatcga
    18421 tgagggaatg agatttgcta ttcttttcgg ttttattttc gagcatctgc gttttctgct
    18481 gcctccaggg tgttccacat gagcatggcg atggtcatgg gaccaacacc tcggggaact
    18541 ggtgttataa aggctgcctt ttcttttact tcatcaaagg caacatcacc gacgagacga
    18601 aaacccgact ttttactttc atcgggaatt ctatttacac ccacatctat aataaccgcg
    18661 tcttttttta tcatatctgc agtaatagta tttgcccttc ccactgctgc aatgagaata
    18721 tctgcttgac gggtaaaaga agcaaggtcc tttgttcctg tatggcaaag cgttactgtt
    18781 gcattgcttt ctctgcgtga aagaagaacc gcaagaggct tcccgacaat atttgagcga
    18841 ccaacaatga ccacatgggc tccctttgta gaaatgccca ttttttccaa taaaacaagg
    18901 acaccatggg gagtacaagg cagaaaggat ttatttccga tgagcatatt gccgacattt
    18961 acgggatgaa agccgtcgac atctttttta gggtcaatcg cctgtatcac agcagcttct
    19021 tctaaatgct ttggaagcgg taattgcaca agaatgccgt ggacgctctc atctttgtta
    19081 agctcgtcga tgagggcgag aagttcttct tggctggtcg ttgcaggaag acgaatagag
    19141 cgatccttca taccaacttc ctgaagcgct ttttcttttc cggtaacata ggaaatactt
    19201 gcggggtcct ctccaacaag cacaacagct aaacaagctt ctatttgttt tgcttttagt
    19261 gcaagcactt tttttgcaac atcagcgcgc acttgagaag ctatttcaaa accatctata
    19321 ataattgcag ccatattttt ctcctaaaga caggtctcgt atatttctac ttgcctatct
    19381 tctttttatt caatatagta gtaactatag caagtctaag tgttttattc tataagaaca
    19441 atataaaaaa atgactacaa ataaaggtga acaaatgaaa aaatatttaa gtctatgttt
    19501 ttgtctttgc tttctaagcc ttgcagtttt cgcacaaatg aatgaggatg agcaagacaa
    19561 ggatgcaaaa acagaagaac tctatgtcta caagatgaac caaaaaggcg actggttcat
    19621 aaaactcggg ggagccgctt ttatccccct ccaacccaaa aatatgaacc tgggcttgga
    19681 cttaaatctc ggtttttatc gatttttatg gagcgaattt tccctcggcg gtgatatt
//
LOCUS       DAOSSW010000027        19461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_2971_length_19461_cov_7.165413, whole genome shotgun sequence.
ACCESSION   DAOSSW010000027 DAOSSW010000000
VERSION     DAOSSW010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 19461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19461
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2971_length_19461_cov_7.165413"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1070
                     /locus_tag="PKW26_03810"
     CDS             177..1070
                     /locus_tag="PKW26_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOQ92727.1"
                     /translation="MKGIPLGERYKHLFGALQANTKAPAVITEENVFQKGNDAILPIV
                     DGMLDENFLEGSRLEGLEHFENFLNLVKEGKRGLILMEHYSNLDLPALIYLLRKDAGA
                     MGEDLAKRIVAIAGMKLNEEDPMVRAWAEAFSRIVIYPSRSLSSIKDPEKYAEEEAKS
                     KKINMASMRALDASRKRGEVTLVFPSGTRYRPGKPETKRGVREIDSYLRLSDVMILVT
                     INGSCLRINEEDPNNMLSDLVIPDCVVLSSSPVFECKAFRNEVINAVNDPEVDKKQAV
                     VDRVMDLLNEQHEEIEKIRFR"
     gene            complement(1074..2459)
                     /gene="hisD"
                     /locus_tag="PKW26_03815"
     CDS             complement(1074..2459)
                     /gene="hisD"
                     /locus_tag="PKW26_03815"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOQ92728.1"
                     /translation="MTIKTLSTENFLRTEAAKAFFSPRFSDNSVEESVKTILEKVASE
                     GDTALHYFSKHFDKLDLHSFEIPLSERKKAASELKKTKPELYKAIQFSYKNALRFAKK
                     QKKSFGNFEMEIAKGVFTGQKNIPVEKAGIYVPAGRYPLFSSLIMGAAPAQAAGVEKI
                     ILCTPPRLHPKDAQEQEHAEKNNKKIALKPWADETTLAVSYICKIDTVYALGGAQAIA
                     AMAYGTETIPQVDVIVGPGNKYVAEAKRLVYGKVGIDFIAGPTEVFIIADESARASWV
                     AADLLAQAEHDPDAQAILATNSEKLIAEVKTEIQKQISLFPEPEAALQSLKENGLILL
                     CKDLDEAIEIANKKAPEHLEIALDDTKERKKLVEKARNYGSLFIGHEAAEVFGDYCAG
                     LNHTLPTSGSARFTGGLSVRHFIKTLTTLRAHNQESIQEIAKHSALIGRSEGLIGHAR
                     AAEIRFQNSQD"
     gene            complement(2468..3769)
                     /gene="hisIE"
                     /locus_tag="PKW26_03820"
     CDS             complement(2468..3769)
                     /gene="hisIE"
                     /locus_tag="PKW26_03820"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOQ92729.1"
                     /translation="MVISSIDLKNGQVVQLKNGKDAVLERNDWQAIIEDFAFYGETAV
                     IDLDAALGNLITEGSEKGNTVNTEIVHKLLRKGNVRVGGGVRSVKKAKELISLGAEKV
                     IIGSSAWKDGALDEVFLTELTKAIGKQRIIISIDAIDGIIAVKGWKETAGIPLLEAAV
                     QAEKWASELLFTCVEREGCMTGFDLALAKKLRKLVSCRLVVAGGVSSLEEIIALEKMQ
                     IDVQLGMALYTNVVSLRDAFVECLNWESAEKNTEGLISVIAQSPSGEVLMNGFANKEA
                     FYKSIDEKKLTFWSRSRNTLWTKGESSGNILKVLQLRADCDRDAVLATVEPQGPTCHT
                     GSWSCFGGEAIASSSFQRLSNIIAERFSNPKPGSYTATLTDTVVREKIEEEAEELIEA
                     EGKDEVVWEAADLLYFISVLLHRENLSWDDVLNELDRRHKK"
     gene            complement(3897..6038)
                     /gene="ppk1"
                     /locus_tag="PKW26_03825"
     CDS             complement(3897..6038)
                     /gene="ppk1"
                     /locus_tag="PKW26_03825"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOQ92730.1"
                     /translation="MNKTLSYLNRELSWIEFNNRVLLEAQKESVPLLEKLKFLLIVSS
                     NFDEFFKVRLAELKKNAVSKPEEKDISGLTNKEQLAKVSERTKTIIANQYSYLNKELM
                     PQLAEKGLAHISQANYSPEDISFLSPFFQDNIFPILTPVRLEEKSETNHISNQKLNVA
                     FLLKSQAENSSMPEALRHKEGELPIATVQIPLSLPRIIWLPEDRGKKRFTLLENLIAN
                     FGTRLFPGYDVEETAFFRLVNDAAFSVDEQRDFDFIQAMEEVLEKRLSSLPVCMHFED
                     ASKTIIQAVSKKLSLTDEYSYEVSGIADISALSGLLDLEGFAELKNAEWKIFPNRYLN
                     LKTNVWDVLKERDVLLNLPYEKFDPVVDLINQASKDAHVLAIKMTLYRTNSQSRIIQA
                     LERAARNGKQVSVFVELKARFDEKQNIEWVTRLEQAGVVVIYGIVHIKVHAKLLLIIR
                     KEYEGIKRYAHFSTGNYNESTAKLYSDLCLFTSKDEYTQDATLFFNTISGYSSIPTLK
                     HLYMAPVNLKEKLLSLIEREIRCSNTLTPGLIMAKMNSLSDKDIIKALYKASQNNVNV
                     LLNVRGICLLVPGVKGQSENISVISIIDRFLEHSRMYYFQNSGEEEIYLSSADCMPRN
                     LERRIELLFPIIDEEVFQNLKDLLLIYFSDNQKAFLLQSDGSWKQRNREKGEEKIRAQ
                     KLIHESFKKKNETLYNNAREFTVRRSQAAET"
     gene            complement(6078..7604)
                     /locus_tag="PKW26_03830"
     CDS             complement(6078..7604)
                     /locus_tag="PKW26_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ92731.1"
                     /translation="MLSIEAVIEIGATGIRLLIAEYTKEKKWQILDHSELPISLGYDV
                     FSNGSVSRESLLECLTILHNYKEQIKSWAIKEDAISIIATSAIREASNKEIILDRIFV
                     KTGFKVKVIDGIEEIRLMYLGVVHAIHNTDLAFQKNNSLIIEVGGGSTEILFLQAGKI
                     TEVHSLRLGTVLIEQHSKASMGSQKEAKEFLRDYIASAADQLSGELRPQSVEVFIAIG
                     SEVRLAASHVGKKISSLAYSIEREAFIHFVDEIQEYAVEEINERFKIPYNEARTLAIS
                     LLTYKLFLSLTPAKMLLVPETSIREGLLIAKREANSSLLQAEFNEQIIAAVYNLGKKY
                     NIDMEHAEYVKKTSLFLFDSMKDELGLKVEERLLLEIAALLHDIGAFIRPQNHNEHSE
                     YIILQSDIFGLNKQKHTMIASATRYHRGPEPQLSDSRYASLTREERVSVLKIASILRI
                     AEALDRSHRQRLKNLEIEFKNDSFFITTEGLESLLEKRALKEKANLFETVFGYRILLL
                     "
     gene            complement(7626..9056)
                     /locus_tag="PKW26_03835"
     CDS             complement(7626..9056)
                     /locus_tag="PKW26_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOQ92732.1"
                     /translation="MNKRDFPKIHFYDQDFVDVYDKTWSWLQDFWFNPETETADLNGL
                     FIYPHSQNPVVDQMESIFSTFFLVYSNKNYAANSNLDFFYKQQEESGAIRWKYSFPSL
                     EAVRPKENPEAVGLPLFSWAEFNLYHKTANKKRIKEIVPHLEKYTQWLDETFKQENGL
                     YAVKHQASTMYNSPRENTYYPVDFNACMAIHALYMAALGDILNDKDINFQYKRMYFSL
                     KTRINKLMWCSDTGFYYDLNKKEEKLPQKTIAGFWPLLAEIPNEDKADALTAHLTNPE
                     TFGTDHPFPSLSADHPKFSVHGNGYCGSVLPEFNFMILKGLEKYHKWDLARESAIRHL
                     YYVLEAVFPGGSHTSGDLWQGYLPNKEGKASGSASQEFPKKRYLQSAALSTIAVMIEN
                     VIGLTISLPRKTVDWIIPNIEIMGIENLSLKRNLITILSNKSNRGWEIQMESEKLYYF
                     TINILGQKKKTLPIPSGKCSMLIDKL"
     gene            complement(9303..11075)
                     /locus_tag="PKW26_03840"
     CDS             complement(9303..11075)
                     /locus_tag="PKW26_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92733.1"
                     /translation="MTTHPFRKIIIHLCVYTVIILGIFAIQFRNQSIISKNFGQLRLS
                     VSQLKKNTDGNTEFDDRFTIQYKGLNIYANNTKPVLIKDKEKTQKLQFLAWESLSDSS
                     FELFFSHNVSLLCGFTGQNKEIFTITSSTNNPDWLEISVPYEIMPTYAVMQTNPKNLI
                     LSGKTQQTILNAAAIDEHYVYFTAQNNNLSYANYEPTKKFMFEEASRYALAKKEVYDQ
                     GVFYLRQKLLTSFDTSENISESQVIALIAEHLERGSYKEGLARIPQSFLNSSRRSYLS
                     SPYFNNLSLMQKSLMMYHENMAYKMQHAQKTKQLDIFQGDSFPVFLLTQNKDKIESLL
                     QTLVNRDSFEPTLLEASGIIESYLLLEQHKRNERKIIEPLLFACIETIQKACTLEDDK
                     LRLIEQETKQNTVSSLRVAKTLINYGIFIKNTDITTTGYFLANSALENTANLTAQNIA
                     ELYPILVQTNYYPHIEILNQANGKPIWAWTSAKNLSLNTDSDGTITLRIEFPVGQSHY
                     IIVHNIEAFEAIEIYEMFFRTDPRFETYDSSGYVYDAANKNLLLKVKNKTAVETIKLY
                     YKTAATETATETTTEKMDENKPEA"
     gene            11141..12559
                     /locus_tag="PKW26_03845"
     CDS             11141..12559
                     /locus_tag="PKW26_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ92734.1"
                     /translation="MKKNKVCIRFCVFLLISFFSACTTTQKIEKGEGIVDGHEVAVES
                     EEEIFSLSDFLDTLYVSLRNNGLEKTLGLFDTLPEEAEGDFTIEYLHASLLFSANDYD
                     AAAEIANRLAVENPNNADVMYLRAMIAKGQGKLTEKNALLKQMIAEDPTNPEANVELA
                     NDQMLRKNFGLAKRYFQTSLSSDPTNPDAMLGLGQSAYYTGDLKMSKDILTRMTEIYP
                     EESMGWFFLAKLAGEEENYKKAGDYIETALSIEPDYYDYWSDYGIYLRYQGRSDEAEK
                     AWSRAIDLNDEAFYAYIHRASLYDEKEDLDKALLDYIKIIELNPDYPYSYESLGMIYW
                     HKEEWAKSRNAFNYAYKLNPANISYPLMISATYLREKNTKANKDFLKNVYGKFPADSI
                     EYAMARLYYDGINPPLVEKKLTSITSSFLKGKMHFYMGLFYDLYGDDINAKKQYLIVK
                     NLQSPMFFEYRLNDWILAKMNG"
     gene            12572..13840
                     /locus_tag="PKW26_03850"
     CDS             12572..13840
                     /locus_tag="PKW26_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOQ92735.1"
                     /translation="MDLEKETEQQEAIEKKTETDGIETAVEKKVLVNTKKTLRFQDRE
                     IILLGTAHVSKESCEQVAEAIAEENPDCIAIELDEERLASMKNPNSWKNLDIVKVLKE
                     NKGFVLLANLVLSSFQKRLGANVGVKPGDEMRAALEEAEKKGIATVMVDRPIQITLKR
                     AWATNSFWGKMKLLSALIASAFETETISAEEIEKLKNSNEMDAMMNELAEYLPSVKKV
                     LIDERDEYLAKKIWTSKGEKILAVLGAGHLPGVERQLEKLSKGETNSDTSAISEIPAA
                     GIGGKIAGYIFPALIVGLIVAGFFTGGAISSLEMLLRWLLWNGSLAALGTLLALGHPL
                     AILVGFFGAPIATLNPVLGVGLFTGLTQAWIRKPKVEDMETLQEDLSSIKKIYKNRIL
                     RVLLVFILSSVGGVIGNFIAVPALFTSLIN"
     gene            13880..15289
                     /locus_tag="PKW26_03855"
     CDS             13880..15289
                     /locus_tag="PKW26_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92736.1"
                     /translation="MNKIQLLSFLLLCLFMPAFVCSEESQSFAYLLENYLENDRSLKE
                     IELQYEQALLSYKKTQVQNSLSISLSSGTSVLSIDENGADLSLAPSLSLGLPEFQNTK
                     AEVKFPMTLPLKENASPSIQDAGLSLSMDIISSAKKNRALVIKKAEESLRDASQKKEA
                     RILAVKKEFLQSLKSIYTLQSNLLTQEDNALTKEVDLSLLKMQGFAESSAKYRLAYLS
                     WQSVLRDYEEAKRSFEKEILDFARKCGTEKFELSIEIPREELVDISEFPKTSFSAIKT
                     SIQNYENNSLAEKAKSPFTLALNAGYGFSLSEIQASRTVKNTLSSGLTFKKDGLSASL
                     GMQNSLEKIENPRFTFSLSYSFLEQALSTIALSEDEVQEKILMQAIENAELTYNSTVQ
                     DKQKQKDDLLWQLQKNKGQEALYADLAEDMKTWYDKGIISTSEYKQALTNYAKASTQV
                     RISEIDGIIYNIDIALLFY"
     gene            15363..15602
                     /locus_tag="PKW26_03860"
     CDS             15363..15602
                     /locus_tag="PKW26_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92737.1"
                     /translation="MSKKRNKIAKKRRIILIVSILLIVLALILLILGLRAKNTGAKKV
                     QASEYRVREESFENIIEITGNVLAAASQDLTIAGS"
     gene            15618..16451
                     /locus_tag="PKW26_03865"
                     /pseudo
     CDS             15618..16451
                     /locus_tag="PKW26_03865"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            16535..17230
                     /locus_tag="PKW26_03870"
     CDS             16535..17230
                     /locus_tag="PKW26_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ92738.1"
                     /translation="MDAVKRVYEMGDNVVEALRGVDFKVQQGEFLSIMGPSGSGKSTC
                     MNMIGCLDRPTSGDVIINGKKTKLMNEKELARLRNETVGFVFQQYHLLSTMTVLENVM
                     LPLRYKGIPLTERRILAKETLQSVGLEDRLSHRPHELSGGQKQRVAIARAMVTNPRII
                     LADEPTGALDSKTGKQVLDLFWDINRTGTTIIIVTHDPGIGLSLPRAIHLFDGLISED
                     GKQHSLEKEVHHV"
     gene            17223..18413
                     /locus_tag="PKW26_03875"
     CDS             17223..18413
                     /locus_tag="PKW26_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ92739.1"
                     /translation="MFEDFYNALQNFKLNKTRTFLSLLGIIIGVASVIVITTLGQSAT
                     DNIKKSFGSSGLDQVSLSQGFMNRRGVTNITFNDSFRSELWDYIPHLKGIYYNNSLSA
                     TLMYGDISASANATAIEYGFLEMNNYELEEGTSFSVTDTVEGTQKAIIGSELAESLFP
                     EGSAIGKSVIMTISNVRFGFEIIAVLKSQSSGFSSTGSAVYIPRGFYTKKIMPSQIAN
                     SIVLQLTDQNHATRVVEAIKNYAKEKTGQDNMLQVMSMQSMLDQYDQVLGTVSLLLSS
                     IAAISLLVGGIGIMNIMIVTVTERRKEIGIRKALGASPNAIRLQFLVESASISLMGGI
                     LGILFGLLLSFIATYVLDWVFSVQFVFCLIAFLFSAFVGVFFGLSPAARAAKLDPVEA
                     LASE"
     gene            complement(18466..>19461)
                     /locus_tag="PKW26_03880"
     CDS             complement(18466..>19461)
                     /locus_tag="PKW26_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOQ92740.1"
                     /translation="YVSGEESASQIKSRANRLSIFEDNIEVLCTRLLEDVLEALASIN
                     PVLLIVDSIQTLFSPPMGPVPGTVNQLKYCANELIRWVKERDSVLFLTAHITKDGGIA
                     GPKALEHMVDTVVFFERNDDRTRFLRSSKNRYGSVDELGIFSMTEKGLVPVEDPASLF
                     LTKRREKLPAGVAIVPVFEGSRVFLVEIQALTVPAKGSLSRVYSDKVDSARISRIAAV
                     LEKRLGLCFSDQDLYINVAGGVRLTESAIDLALAMALFSARSDKAIAEKTVFVGELSL
                     AGEVRPVDRLQQRIKASQALGFTRLVAPDFEGAKKLDAVHPISQIREGIDLFFTK"
BASE COUNT         5724 a         3739 c         4022 g         5976 t
ORIGIN      
        1 gattttttga aaatagccat agcaggctat tggcttaaaa aagaacggtg ggtaaaaaac
       61 cttgcccttg acaactaaat aaagacttat taaggttata taggtttttt ttattttatg
      121 ataaaataag ttcggcttaa aacgctgttt tttatatacg attaggagat tataatatga
      181 agggaattcc tttaggagag agatacaaac atttatttgg cgccttgcaa gcaaatacaa
      241 aggcaccggc tgttattact gaggaaaatg tatttcaaaa agggaatgat gctatattgc
      301 ccatcgttga cggaatgtta gatgaaaatt ttctagaggg ttcacggctt gaaggcttgg
      361 aacattttga aaatttttta aacttagtta aagagggaaa gcgtgggctc atcctcatgg
      421 aacattatag caacttagat ttacctgcgc tcatttattt gttacgaaaa gatgcagggg
      481 cgatgggtga ggacctagca aagagaattg tcgccattgc tggtatgaag cttaacgaag
      541 aagatcccat ggtgagagct tgggcagagg ctttttcacg cattgttatt tatccgagtc
      601 ggagtttaag ctctataaaa gacccagaaa aatacgcaga agaagaggca aaaagtaaaa
      661 aaataaatat ggcttctatg cgcgcgctcg atgcgtctcg caaacgtgga gaggtaacgc
      721 ttgtttttcc ttctggaaca cgatatcgtc caggaaaacc tgaaacaaaa cgtggggttc
      781 gcgagataga ttcatatctg cgcttgtccg atgtaatgat tttggttacg ataaatggtt
      841 cttgcttacg tatcaatgaa gaagatccaa ataatatgct cagcgacctt gttattcccg
      901 attgtgtcgt tctttcttca agtcctgttt ttgaatgtaa agcatttcga aatgaggtaa
      961 tcaatgcggt aaacgatcct gaggtcgata aaaaacaagc ggtcgtcgat agagttatgg
     1021 acttacttaa cgaacaacac gaggaaatag aaaaaatacg ctttcgctaa cttttagtct
     1081 tggctgtttt gaaagcggat ttctgcagca cgggcatgac cgatgagccc ctcgcttcgt
     1141 ccgatgaggg ccgagtgttt tgctatttct tggatgcttt cttgattatg ggctcgtagc
     1201 gtagtgagtg tttttataaa atgccgcacg ctcaagcctc ctgtaaaacg ggcggaacca
     1261 gaagtgggaa gcgtgtggtt taagccagcg caatagtcgc caaaaacttc tgctgcctca
     1321 tgccctataa aaagagaacc ataatttctt gccttttcga cgagtttttt tctttctttt
     1381 gtgtcatcga gggctatttc aagatgctcg ggtgcttttt tatttgcaat ttctatggcc
     1441 tcatctaaat ctttgcaaag aaggatgagt ccgttttctt ttaaactttg tagagccgct
     1501 tctggttcgg gaaagaggct gatttgtttt tgtatttcag tctttacttc tgcaatgagc
     1561 ttttctgagt ttgttgcaag aattgcttgg gcatcagggt cgtgttctgc ttgtgcgaga
     1621 aggtccgctg ccacccatga ggctcgggcg ctttcatccg cgatgataaa gacttctgtc
     1681 ggtcctgcaa taaaatcaat gcctaccttc ccgtatacga gtcgcttcgc ttctgcgaca
     1741 tatttatttc ctggtccaac aattacgtca acttggggaa tagtctctgt tccataggcc
     1801 atagctgcaa tcgcttgggc gccgccgagg gcgtagacag tatcaatttt acaaatatat
     1861 gaaaccgcaa gcgttgtttc atccgcccac ggttttagcg ctattttttt attgtttttt
     1921 tctgcatgct cttgttcttg tgcatctttg ggatgcaggc gaggcggtgt gcaaagaatt
     1981 attttttcta caccagcagc ttgtgcggga gcggctccca ttatgagcga ggaaaaaagc
     2041 ggatagcgtc cagcagggac atagatgccg gctttttcta caggaatatt tttttgtccg
     2101 gtaaaaacac cttttgcaat ttccatttca aagttgccaa aggatttttt ttgttttttt
     2161 gcaaagcgaa gggcgttttt atatgaaaat tgaatagcct tatagagttc gggttttgtt
     2221 ttttttagct cagaagcagc cttttttcgc tcgcttagcg gtatttcaaa agaatgcaag
     2281 tcaagtttgt caaagtgttt tgaaaaataa tgcagggcag tatctccctc ggaggctact
     2341 ttttccaaaa ttgtttttac actttcttca acggaattat ctgaaaagcg aggcgaaaaa
     2401 aaagcctttg cagcctctgt cctcaaaaaa ttttctgtgc taagcgtttt tattgtcatg
     2461 ttttttccta ttttttatgt cttcggtcga gttcgtttaa gacatcgtcc caacttaggt
     2521 tttctctatg caaaagaaca gagataaaat agaggaggtc ggctgcttcc caaaccactt
     2581 cgtcttttcc ctctgcttct ataagctctt cagcttcttc ttctattttt tcacgaacta
     2641 ccgtatcagt taaggttgcg gtataggagc ctggtttggg atttgaaaaa cgctcggcaa
     2701 taatattgga gagtctttga aagcttgaag atgcgattgc ttctccgcca aaacatgacc
     2761 agcttcctgt gtggcatgtt ggcccttgag gttcgactgt cgccaaaacc gcatcgcgat
     2821 cgcagtcagc acgaagttgt aaaaccttga gaatatttcc agaagattcg ccctttgtcc
     2881 acaaagtgtt tcgagagcga ctccaaaagg tcagtttttt ttcatcgatg cttttataaa
     2941 aggcttcttt gtttgcaaag ccattcataa gcacctctcc tgagggactt tgtgcgataa
     3001 ctgaaataag cccctctgta tttttctcag cgctttccca gttgaggcat tctacaaagg
     3061 catctcgcag ggaaacaaca tttgtgtaca gggccattcc gagctgcacg tcaatttgca
     3121 ttttttcaag tgctatgatt tcttctaggc tggaaacgcc gcctgctact acgagtcggc
     3181 aagagaccag tttgcgcagt ttttttgcga gcgctaggtc gaagcccgtc atacaaccct
     3241 cgcgttcaac gcaggtaaag agcaactcgc ttgcccactt ctctgcttgc acagcggctt
     3301 ccaaaagcgg tatgccagcc gtttccttcc agccctttac ggcgatgatt ccgtcgattg
     3361 catctataga aataataatg cgttgttttc caattgcctt tgtaagctca gttaaaaaaa
     3421 cttcgtcaag cgcgccgtcc ttccatgcag aagagccgat gataactttt tcagcaccaa
     3481 gggagataag ttcctttgct ttttttacac tgcgaacacc tccaccgact cgaacatttc
     3541 cttttcgtaa gagcttgtgt acgatttcgg tatttacggt gtttcctttt tcactgccct
     3601 ctgttatgag gttgccgagt gcggcatcga ggtctatcac tgcggtttcg ccataaaagg
     3661 caaaatcttc tataattgcc tgccaatcat tacgctctag aacagcatct ttcccatttt
     3721 tgagttgaac aacctgtcca ttttttaaat ctatcgatga aataaccatg ttaaaaagta
     3781 taacaaaaag cccccctttg ggcaataaag agatctttgc ttgtgggaat ttcaaaaaat
     3841 tctgcgttaa aactttaaac cttttttcta gattttataa aacaataagg catgttttat
     3901 gtttcggcag cttggcttct tcgaacggta aattcacgcg cattattgta taatgtttcg
     3961 tttttctttt taaaagattc atgtatgagc ttttgagccc gtattttttc ttcgcctttt
     4021 tccctgtttc gctgtttcca agagccgtca gattgtaaga gaaaggcttt ttggttatcg
     4081 ctaaagtata ttaaaagcaa gtcttttagg ttttggaaaa cttcttcatc tatgatggga
     4141 aaaagcaatt caatacgcct ttctagattt cggggcatgc aatcggcact tgaaaggtag
     4201 atttcctctt cgccagaatt ttgaaaataa tacattcgtg aatgttccaa aaaccgatcg
     4261 ataatgctaa tgacagaaat attttcgctt tgtcccttta caccaggaac gaggaggcaa
     4321 atacctcgga cattgagcaa gacattgacg ttgttttgac tcgccttgta cagtgctttt
     4381 ataatatcct tatctgagag gctgttcatc ttcgccataa taagacccgg tgtaagggta
     4441 tttgaacagc ggatttctcg ctctatgagc gataaaagct tttctttaag gtttacaggg
     4501 gccatataaa gatgctttag tgtaggtatg gacgagtacc ctgaaatagt gttaaaaaaa
     4561 agtgtggcat cttgggtgta ttcgtccttg cttgtaaata aacaaaggtc gctgtagagt
     4621 tttgcggtag actcgttgta atttcctgtt gaaaaatgag cgtagcgttt tataccttcg
     4681 tattcttttc gtataatcag taagagtttt gcgtgtactt tgatatgcac tatgccatag
     4741 ataaccacta ctcctgcttg ttcaagccgc gttacccatt ctatattttg tttttcatcg
     4801 aagcgggctt taagctcgac aaaaacgctt acttgctttc catttcgtgc agcacgttct
     4861 agcgcttgaa taatacgtga ctgggaattt gtgcggtaaa gtgtcatctt tatagcaaga
     4921 acatgggcat ccttactcgc ttgatttata agatctacaa cagggtcgaa tttttcatag
     4981 ggtaggttca aaagtacatc acgttctttt aaaacatccc atacatttgt ttttagattt
     5041 aagtagcgat tggggaaaat tttccactca gcatttttaa gttcagcaaa accctctaag
     5101 tccagcaagc cactcaaggc tgaaatatct gctatgccag aaacctcata gctatattcg
     5161 tcagtgagag agagtttttt agaaaccgct tgtattattg ttttgcttgc atcttcaaag
     5221 tgcatacaga caggcaaaga agaaagacgt ttttccaaaa cctcttccat tgcttggata
     5281 aaatcaaagt ctcgttgttc atcgactgaa aatgcggcat catttacaag ccgaaaaaat
     5341 gcggtttctt ctacatcgta gcctgggaat aaacgcgtcc caaagtttgc aatgaggttt
     5401 tccaaaaggg taaaacgttt ctttcctcta tcttctggta accatataat tcggggaaga
     5461 gagagaggga tttgaacggt agctataggc aattcacctt ctttatggcg aagcgcctca
     5521 ggcatagagg agttctcagc ctgcgattta agtaaaaagg cgacgtttag tttttggttc
     5581 gatatgtgat ttgtttcgct tttttcttct aaacgtactg gtgtgagaat gggaaatata
     5641 ttgtcttgaa aaaaggggct taaaaaactg atatcttctg ggctatagtt tgcttgagat
     5701 atatgggcga gacctttttc agccaattgc ggcattagct ctttatttag atagctatat
     5761 tgatttgcta taatagtttt tgttcgctct gaaaccttgg ccaattgctc tttgtttgta
     5821 agtccagaaa tatctttttc ttcaggtttt gaaacagcat tttttttaag ctcggcgaga
     5881 cgcactttaa aaaactcatc gaagtttgag cttacaatca agagaaattt taacttttcc
     5941 aaaagaggca cagattcttt ttgtgcttct aagagcacgc gattgttaaa ttcaatccaa
     6001 gagagttctc tgtttaagta gcttaaggtt ttattcatac gcattccgtt ttgttcgatg
     6061 atagcgtatt ttaatcttta gagcaataaa attctatagc caaaaactgt ttcaaagaga
     6121 tttgcctttt ctttcaatgc gcgtttttcg agaagacttt ccaaaccttc tgtcgtgata
     6181 aaaaaactgt cgtttttgaa ttcgatttct agatttttaa gtcgctgtct atggcttctg
     6241 tccagagctt ctgcgatacg caagatactt gcaattttca aaacgcttac ccgttcttcg
     6301 cgagtgaggg aggcgtaccg cgagtcagat aactgcggtt caggaccacg gtggtagcgc
     6361 gttgcacttg caatcattgt atgtttttgt ttgtttaaac caaaaatgtc tgactgcaag
     6421 ataatgtatt cgctgtgctc gttgtggttt tgtgggcgaa taaaggcccc aatgtcgtgt
     6481 aagagagctg caatttctaa gagcaagcgt tcttcaactt ttaagccaag ttcatctttc
     6541 atactatcga ataaaaacaa agacgttttt tttacatact ctgcatgttc catatcgata
     6601 ttgtattttt tcccgaggtt ataaacggca gcaataattt gttcgttaaa ctcggcttgc
     6661 aagagactgc tgtttgcctc tcgtttggct atgagcaaac cctcacgtat tgacgtttct
     6721 ggaacgagaa gcattttcgc aggagtgaga cttaaaaaaa gcttataggt gagaagagaa
     6781 atagcgagtg ttcttgcttc attataaggg attttaaaac gctcatttat ttcttcgacc
     6841 gcatattctt gtatttcgtc aacaaagtga ataaaggctt cgcgttctat cgaataggcg
     6901 agagatgaaa tttttttccc gacatggctt gctgcaagac gtacttcaga accaatggca
     6961 ataaaaactt cgactgattg cggcctcagt tcaccgctca attgatcagc ggcactcgca
     7021 atatagtcac gtaaaaactc ctttgcttct ttttgggaac ccatcgaggc cttactatgt
     7081 tgttcgatga ggacagtacc gaggcgtaaa ctatgtacct cggtaatttt tccagcttgt
     7141 aaaaagagta tttctgttga gccgccacca acttcgatta tgagtgagtt atttttttga
     7201 aaagcgaggt cggtgttatg tatggcatga acgacaccga ggtacatgag tcgaatttct
     7261 tctatgccat cgattacctt gactttaaat cctgttttga caaaaatcct atcaaggatg
     7321 atttctttat tgcttgcttc acggattgca gaggtcgcaa taatcgatat tgcatcctct
     7381 tttatcgccc agctttttat ttgctctttg taattatgta aaatagttaa gcattctaag
     7441 agcgattccc ttgaaacaga accgtttgaa aaaacatcgt agccaagaga aatgggcaac
     7501 tcggagtgat cgagtatctg ccattttttt tccttggtat actcagcaat gagaagccga
     7561 attcctgttg cacctatttc aataaccgct tcgatagaaa gcacaatgaa accccatttt
     7621 tagaactata atttatctat gagcatagaa cacttacccg acggaatagg taaggttttt
     7681 ttcttttgcc ccaaaatatt gatagtaaaa taatagagtt tttcgctttc catttgtatt
     7741 tcccaaccgc gattactttt atttgataaa atagtaatga ggtttcgttt gagcgagagg
     7801 ttttcaattc ccataatttc aatattgggt ataatccagt caactgtttt tcgaggcaag
     7861 ctaattgtga gccctataac gttttctatc atcacagcga ttgtcgaaag ggcagctgat
     7921 tgtagatagc gtttttttgg aaattcttga cttgcggaac cgcttgcctt gccctctttg
     7981 ttgggcaggt agccttgcca gaggtcgcca gaagtatgtg agcccccagg gaaaacagcc
     8041 tcaagtacat aatacaaatg tcgtatagct gattcacgtg ccaaatccca tttgtggtat
     8101 ttttctaagc ctttgagaat cataaagtta aactcgggta aaacagaacc gcagtagcca
     8161 ttgccatgaa cactaaattt tgggtgatcg gcagaaaggc ttgggaaggg atggtcagtt
     8221 ccaaaagttt cgggatttgt taaatgtgcg gtgagtgcat ctgccttatc ttcgttcgga
     8281 atttctgcca aaagaggcca aaaacctgca attgtttttt gcggtaattt ttcttctttt
     8341 ttatttaagt cataataaaa accagtatct gagcaccaca taagcttatt gatacgagtt
     8401 tttaaggaaa aatacattcg tttgtactga aagttgatat ccttatcgtt taaaatatcg
     8461 ccaagggcag ccatatagag ggcatgaatc gccatgcagg cattgaaatc gacggggtag
     8521 taggtgtttt cccgtgggga attatacatt gtcgatgctt gatgttttac cgcataaagt
     8581 ccgttttcct gtttaaaggt ttcatcgagc cattgtgtgt atttctccaa gtgagggaca
     8641 atttccttta tacgtttttt atttgctgtt ttatgataga gattaaactc tgcccaggaa
     8701 aaaagtggaa gccccacagc ttctggattt tccttcgggc gaactgcctc gagacttgga
     8761 aagctgtact tccagcgaat tgcaccgctt tcttcttgtt gtttatagaa gaagtcaagg
     8821 ttggaatttg cagcataatt tttatttgag taaaccaaaa aaaaggtcga aaaaatagac
     8881 tccatttggt ctactacagg gttttggctg tgaggataaa taaataatcc atttaagtca
     8941 gctgtttctg tttcagggtt aaaccaaaaa tcttgcaacc aagaccatgt tttatcatac
     9001 acatctacaa agtcttggtc ataaaagtgt atttttggga aatcgcgttt gttcacagat
     9061 tctcctttaa tatattggaa aaaagacaga aattgttttt ttcgagtata tcacatattt
     9121 ttaaaaagtg atacagtaaa gaaataaaaa ctttgttttt ttaagaatac tcaactttag
     9181 ttccatgttt tttagatata cgaattctcc tatcaccaat aaaacatgta tttccgcctt
     9241 ctgttacaat tattaataaa aaagacgaga tttcccgctt tattgtacat cgttacaaga
     9301 tcttatgctt caggtttatt ttcatccatt ttttcagtag ttgtttcagt agcagtttca
     9361 gtagctgcag ttttataata aagttttatg gtttcaaccg cagttttgtt ttttactttc
     9421 aacagaaggt ttttatttgc agcatcatag acgtagcctg aagaatcgta ggtttcaaag
     9481 cgtggatcag ttctaaaaaa catttcgtat atttcaattg cttcaaaggc ttctatattg
     9541 tgcactataa tataatggct ttgccccact ggaaattcaa ttcgcaatgt aattgtacca
     9601 tctgaatcgg tgtttaaact tagattcttt gccgaagtcc atgcccagat aggtttgcca
     9661 tttgcctggt taagtatttc tatatgtggg tagtagtttg tttggacaag gatggggtag
     9721 agctccgcaa tattctgtgc cgttagattt gctgtatttt ctaaagctga atttgctaaa
     9781 aaataccctg tcgttgttat atctgtgttt tttataaaga tgccgtagtt tataagtgtt
     9841 tttgcaactc gtaaactgga gacagtattc tgttttgttt cttgttctat aagccttagt
     9901 ttgtcatctt ctagtgtaca tgccttttgt atcgtttcta tacaagcaaa taaaagtggc
     9961 tcgataattt ttctttcatt tcgcttgtgc tgttctaaaa gaaggtagct ttctattatg
    10021 ccgcttgctt ctaaaagagt cggctcaaaa ctgtctcgat ttacgagggt ttgtaagagt
    10081 gattcaattt tgtctttgtt ttgggtaaga agaaatacag gaaatgaatc tccttgaaag
    10141 atgtcgagtt gttttgtttt ttgtgcatgt tgcattttat atgccatgtt ttcatggtac
    10201 atcataaggc ttttttgcat aaggctaaga ttgttaaaat agggactaga aagataggag
    10261 cgtcttgagc tattcaaaaa gctttgggga atacgtgcaa gcccttcctt atagctgcca
    10321 cgttctaaat gctcggcgat gagggctatt acctgggatt cgcttatgtt ttcagatgta
    10381 tcaaaactcg tgagaagttt ttgcctaagg taaaaaactc cttgatcgta gacctctttt
    10441 tttgcaagtg catagcgaga tgcttcttca aacataaatt tttttgttgg ctcatagttt
    10501 gcatagctta gattattatt ttgagctgtg aagtagacat aatgctcatc tatagctgca
    10561 gcgtttaaaa ttgtttgttg cgtttttccg cttaggatga ggtttttagg atttgtttgc
    10621 ataactgcat aagtcggcat gatttcatag ggcacagaga tttcgagcca gtcaggatta
    10681 tttgtgctgc ttgttatagt aaaaatctcc ttgttttgtc cagtaaaacc gcataaaaga
    10741 ctgacattat gagaaaaaaa gagttcgaag gagctgtctg agagcgattc ccaggctaaa
    10801 aactgtaact tctgtgtttt ttctttgtct ttaattaaaa caggctttgt attgttggca
    10861 tatatgttga gacctttata ttgtatagta aatctatcat caaattctgt gtttccatca
    10921 gtgttttttt taagctgaga aacgcttaag cgtaattgac cgaaattctt tgatattata
    10981 gactggtttc taaactggat tgcaaaaata ccgagaataa taactgtata tacacaaaga
    11041 tgtataataa tctttcgaaa tggatgcgta gtcataagtg ctagtgtagc taatgattgc
    11101 gacaaaatca actattcaaa aaaaataaag taagggaaaa atgaaaaaaa ataaagtatg
    11161 tatacggttc tgtgtttttc ttttgataag ctttttttct gcatgtacga cgacacaaaa
    11221 aatagaaaag ggagaaggta ttgttgatgg tcatgaagta gccgttgagt cagaagaaga
    11281 gattttttcg cttagcgatt ttcttgatac gctttatgtt tctctgcgaa acaatggctt
    11341 ggaaaaaaca ctcggcttat ttgatactct gcctgaagaa gcagaaggcg attttacgat
    11401 agagtatcta catgcatcgc ttttattttc tgcaaatgac tacgatgcag cagctgaaat
    11461 agcaaatcgt cttgcagttg aaaatccaaa caatgccgac gttatgtact tgcgtgcaat
    11521 gatcgccaag gggcagggta agcttacgga aaaaaatgcc ttgttgaaac agatgatagc
    11581 tgaagacccg acaaaccctg aagcaaatgt tgagcttgca aatgatcaaa tgctgcgaaa
    11641 aaactttggg cttgcaaaac gatattttca aacgagtcta tcttctgacc caacaaaccc
    11701 cgatgcgatg cttggcttag gtcaaagtgc ctattatacc ggggacttaa aaatgtcaaa
    11761 agatattctt actcgtatga cagagatata tccagaggaa agcatggggt ggttttttct
    11821 tgcaaagctt gcgggcgaag aagaaaatta taaaaaagca ggtgattata ttgaaacagc
    11881 actttcaata gaacctgatt attatgatta ctggtcagat tatggtattt atttgcgcta
    11941 ccaaggtcgc tctgatgagg ctgaaaaggc atggtctcgt gcaattgact taaacgatga
    12001 ggctttttat gcctatattc acagggcttc tctctatgat gaaaaagaag acctcgataa
    12061 agcgctttta gattatatta agattattga attaaaccct gattatccct attcctatga
    12121 atccttgggt atgatttact ggcacaagga agaatgggca aaaagcagaa atgcctttaa
    12181 ttacgcctat aaactaaacc cagcgaatat ttcctatccg cttatgattt ctgcaaccta
    12241 tttgcgagaa aaaaacacaa aggcaaataa agattttctc aaaaacgtgt atgggaagtt
    12301 tcctgcagat tctattgaat atgcgatggc tcgtttatac tatgatggca ttaaccctcc
    12361 gcttgtagaa aaaaaactga caagtataac gagtagcttt cttaaaggaa aaatgcattt
    12421 ttatatgggt ttgttttatg acctatatgg cgatgacatc aatgcaaaaa aacaatatct
    12481 tatagttaag aatcttcaaa gtccaatgtt ttttgaatat cgattgaacg actggattct
    12541 tgcaaaaatg aatgggtaaa acgaggaaaa aatggattta gaaaaagaaa cagagcaaca
    12601 agaagctata gaaaaaaaaa ctgagacaga cggaatcgaa actgctgttg aaaaaaaagt
    12661 tcttgtaaat acaaaaaaaa ccttacgatt tcaagataga gaaattattc ttttagggac
    12721 cgctcatgtt tctaaagaga gttgcgaaca agttgcagag gcaattgccg aagaaaaccc
    12781 tgactgcatt gcgattgaat tggatgaaga gcgacttgct tctatgaaaa atccaaactc
    12841 gtggaaaaat ctcgacattg taaaagtact taaagaaaac aagggttttg tccttctggc
    12901 aaacctagtc ttatcaagct ttcaaaaacg tctcggtgcc aatgttggag taaaaccagg
    12961 tgacgaaatg cgtgctgccc tggaagaagc tgaaaaaaaa ggtattgcaa cggtgatggt
    13021 cgaccgcccg atacaaataa ccctgaaacg tgcttgggcg acaaactctt tttggggaaa
    13081 aatgaaatta ctttcagccc tcattgcgag tgcctttgaa acagaaacaa tatctgcaga
    13141 agaaatagaa aagctcaaaa attcaaacga aatggacgct atgatgaatg agcttgctga
    13201 gtatttaccc tcggtaaaaa aagtgctcat cgatgagcga gatgagtatc ttgcaaaaaa
    13261 aatatggacg agtaagggcg aaaaaattct cgctgtttta ggtgctggcc atttacctgg
    13321 cgtcgagcga caactagaaa aactttcgaa gggagagaca aactcggata caagtgcaat
    13381 ctcagaaatt cccgcagcgg gaatcggtgg aaaaattgcg ggctatattt tccctgccct
    13441 catcgtcggt ctcattgtcg cgggtttttt taccggaggc gctatttctt ccctggaaat
    13501 gcttttacgt tggcttttgt ggaatggctc ccttgccgcc ttgggtaccc tgcttgctct
    13561 cggacaccca cttgcaattc ttgtcggttt ttttggcgct cccattgcaa cgctgaatcc
    13621 cgttttgggt gtagggcttt ttacggggct tactcaagct tggattcgta aaccaaaggt
    13681 tgaagatatg gaaacgctac aagaagattt gtcttcaata aagaaaatct ataaaaaccg
    13741 tatccttcgt gtcttattag tgtttatatt atcatcagtg ggtggcgtaa ttggcaactt
    13801 tatcgctgtg cccgcccttt ttacgagtct tattaactag ctaccaactt catgtggttg
    13861 gtctgcatgg gggaatatat tgaacaaaat acagcttctc agttttcttt tgctttgtct
    13921 ttttatgcct gcttttgtat gctcagaaga atctcaaagc tttgcatatc tcttggaaaa
    13981 ttatcttgaa aacgatagaa gccttaaaga aatcgagcta caatacgaac aagcgctcct
    14041 atcctataaa aaaacacagg tacaaaactc actgagcatt tctctttcgt ccggcacctc
    14101 agtcctatca atcgatgaaa atggagccga cctctcgctc gccccttcgc taagccttgg
    14161 gcttcccgag tttcaaaata caaaggcgga agtaaaattt cctatgaccc ttccgctcaa
    14221 agaaaacgca tcgccttcta ttcaggatgc agggctctcg ctttctatgg atattatctc
    14281 aagtgcaaaa aaaaatagag cacttgttat aaaaaaggcg gaagaaagcc ttcgcgatgc
    14341 ttctcaaaaa aaagaagcac ggatccttgc cgttaaaaaa gaatttttgc aatcacttaa
    14401 atcgatatat accctgcagt cgaatctttt aacacaagaa gacaatgctt tgaccaagga
    14461 agtcgattta agcttgctga aaatgcaggg ctttgcggaa tcttcggcaa aatatcggct
    14521 cgcctatcta agctggcagt ctgttttgcg cgattatgag gaagcaaaac gtagctttga
    14581 aaaagaaatc ctagactttg caagaaaatg cggtacagaa aaatttgaac tttcgataga
    14641 aatacctcga gaagaactgg tcgatataag cgagtttcca aaaacgtctt ttagcgcgat
    14701 taaaacaagc atacaaaact atgaaaataa tagccttgca gaaaaagcga aaagtccttt
    14761 tacacttgcc ctcaatgcag gctacggttt tagcttgagc gaaatacagg catcgcgtac
    14821 tgtaaaaaac accctttctt ctggacttac atttaaaaaa gacggtttat cagcttctct
    14881 tggcatgcaa aacagcttag aaaaaatcga aaacccgcgt tttacctttt ctctttccta
    14941 ttcttttttg gaacaagcac tttcgacgat tgctctttca gaagacgagg tacaagaaaa
    15001 aatccttatg caggcaatcg agaatgccga gctaacatac aattctaccg tgcaggataa
    15061 gcaaaaacaa aaagatgatt tgctatggca attacaaaaa aacaagggcc aagaagcctt
    15121 gtatgcagat ttagcggagg atatgaaaac ctggtatgat aaaggcatta tttctacaag
    15181 tgaatacaaa caagctctta caaattatgc aaaggcgagc acccaggtcc gtatatctga
    15241 aatcgatggc attatctaca atatagatat cgccttgctt ttttattaag caaaatcaaa
    15301 aaagtaaagc tcatataaag actgataacg aaggctaaaa ttcgacaaaa aaggaataga
    15361 aaatgtcaaa gaaaagaaat aaaatagcaa aaaaaagacg catcatacta atcgtgagta
    15421 ttttactcat agtgcttgct ttaatcctac ttatcctggg cttgcgggca aaaaatactg
    15481 gtgcaaaaaa agtacaagca agtgaatatc gtgttcgaga agaaagtttt gaaaatatta
    15541 ttgaaataac cggaaatgtg cttgctgcgg caagtcaaga tttaaccatt gccggaagct
    15601 gaactgttac aaaggtctat gttcaaaaag gcgatgttgt aaaaaaaggt caggtaataa
    15661 tcgaacttga tgcaagcgaa caggaatatg cgcttgcaaa gcacgactac gataccgaac
    15721 aaaagcgaat aaatggctct cggcgtgaaa ttgaacttat ggaaaagcaa cgagatgttt
    15781 tgcttaaaaa acttgaagac agaaaaatcc tagcctactt tgatggtatt atcgcacagt
    15841 tttctgttgc cgagggcgat tatttagaag caaaagatag tgtcggtgtt attatcgacc
    15901 gttcatatat gaaggcgatg gttgaagttg ttgaaaccga tgcacaaaaa ttaaagctta
    15961 atcaaaaagt gtattttacc tttcctgcct atgcaaataa aacgattgaa ggctatgtcg
    16021 tggcatatcc ctcagtcggg cgcattacaa accgcggcgc aacagttgtc gacgctgagc
    16081 tacgaatcga taatcctcca gcggatattt tgcccaatta ttcttttacg ggaaaaatcg
    16141 aagtagctgc tccagttaca atgcgtattg ttgagcgaga agcaattgga cggaatggtg
    16201 gaaaatcctt tgtcgaaaaa atacagcctg atggaagcct tgtcaaaagc gatgttgaag
    16261 ttcaagccta tggggtacaa tttgtagaag tcctttctgg agctcaagcc ggtgacctgc
    16321 taaaaagcca aagcacaggg atttctggct cccttcgttt aaattctgct aatacatcta
    16381 caactataaa tagaacgacg ggaggagggc aaggcatgag tggtggcgct gttcctatga
    16441 tgcgtcctta gagtataaga aagtgagaaa aatcgtgaaa aaaaagactg aagaagctga
    16501 aaaaccgctt gtagatacaa atacaattat tcaaatggac gctgtaaaac gagtttatga
    16561 aatgggcgac aatgtcgttg aagcgcttcg aggagtagat tttaaggttc agcaaggaga
    16621 atttctttcc attatgggac cttctggctc tggaaagtca acctgcatga atatgattgg
    16681 ctgtctcgac agaccgacga gtggcgatgt aattatcaat ggaaaaaaaa caaaactcat
    16741 gaatgaaaaa gagcttgcac gcttgagaaa tgaaacagtc ggttttgttt ttcagcaata
    16801 ccacctcctt tctacaatga cggttttaga aaacgtaatg ctcccccttc gttacaaggg
    16861 aatcccgctt acagaaagac gcatacttgc aaaagaaacc ctgcagtcag tcggtctcga
    16921 agatcgcctt agccatcgac cgcatgagct ttctggaggg caaaaacagc gagttgccat
    16981 tgcacgagct atggtaacaa atccccggat tattcttgca gacgaaccaa ccggcgccct
    17041 cgattcaaaa acaggaaagc aagtactcga tttgttttgg gatataaatc gtactggcac
    17101 gacgattatc attgttaccc atgaccctgg gatagggctt tctcttccac gtgcaattca
    17161 cctttttgac ggacttataa gcgaagacgg aaaacagcat agccttgaaa aagaggtaca
    17221 ccatgtttga agatttttac aacgccttac aaaactttaa gctcaataaa acacgtactt
    17281 ttttatccct ccttgggatt ataattggag ttgcttcggt tattgtgatc acgacacttg
    17341 gacaaagtgc cacagacaat ataaaaaaat cttttggctc ttctggtctc gatcaggtaa
    17401 gcttgagcca gggatttatg aatcggcgtg gagttacaaa cattactttt aatgacagct
    17461 ttcgctctga actctgggac tatatacccc atctaaaagg tatttattat aacaatagcc
    17521 tatccgcgac tctcatgtat ggggatattt ctgcttcggc aaatgcgact gctattgagt
    17581 atggtttttt ggaaatgaat aactacgagc ttgaagaagg gacttccttt tctgttaccg
    17641 acactgtcga aggcacacaa aaagctatta tagggagcga acttgccgag tctttatttc
    17701 cagagggaag cgctatagga aagagcgtca tcatgacaat ctcaaatgtt cggtttggct
    17761 ttgaaataat cgctgtttta aaatcgcaaa gctcaggttt ttcttcaaca ggctcagccg
    17821 tctatatacc acgaggtttt tatacaaaaa aaataatgcc atcgcaaatt gcaaactcaa
    17881 ttgttttgca gttaacagat caaaaccatg caacaagggt tgttgaggct ataaaaaact
    17941 atgcaaaaga aaaaactggc caagacaata tgctgcaagt tatgtcaatg caaagcatgc
    18001 tcgaccaata cgaccaggtt ttaggtacag tgagcttgct cttatcgagt atcgcagcta
    18061 tttccctcct tgtcggcggc attggtataa tgaacataat gatagtaact gttaccgaaa
    18121 ggcgaaagga aatcggcata cgaaaagccc tcggagcaag tccaaatgca attcgtttac
    18181 agtttttagt tgaatccgcc tccatttctc tcatgggtgg cattctcggc attcttttcg
    18241 gcttgctctt gagctttatc gcaacctatg tactggattg ggttttctcc gtgcagttcg
    18301 ttttttgcct tattgccttt ttgttttcag cctttgtcgg cgttttcttt ggactcagcc
    18361 ccgcagcaag ggcagcaaaa cttgaccccg tcgaagccct cgcaagcgaa taaaagcaaa
    18421 aaaaagttta agtagcgttt ttacaaacag gtgaaaaaaa attgcttatt ttgtaaaaaa
    18481 caaatcaatt ccttctcgta tttgcgaaat aggatgcacc gcgtctagtt tttttgctcc
    18541 ttcaaagtcc ggtgcaacaa gccgtgtaaa accaagcgct tgggaagctt ttattcgttg
    18601 ttgcaatcga tctacgggac gaacttcgcc tgcgagacta agttccccaa caaagactgt
    18661 tttttctgca atcgctttat cgctccgtgc agaaaacaag gccattgcga gggcaaggtc
    18721 tatggcgctc tcagttaagc gaacaccccc cgcaacattt atatacaaat cttgatcgct
    18781 aaagcaaagt cccaagcgtt tttccaagac agccgcaata cgactgatcc gtgcagagtc
    18841 aaccttgtca gaatacaccc gacttaaaga gccctttgcc ggtactgtca gcgcttgaat
    18901 ttcaactaaa aacacgcgac ttccctcaaa aacaggcacg attgcaaccc ctgcaggcag
    18961 cttttctcgg cgttttgtca aaaacaaaga agcaggatct tcaacaggca ccaagccttt
    19021 ttcagtcatg gaaaaaatac ccaattcatc cacagaacca taacggtttt ttgacgatcg
    19081 caaaaatcgc gttcgatcat catttcgttc aaaaaaaaca accgtatcga ccatgtgctc
    19141 taaagccttt ggtcctgcaa ttcccccatc cttggtaata tgggcggtta aaaataaaac
    19201 agaatcacgc tccttaaccc agcgaataag ctcatttgca caatatttta gctgatttac
    19261 agtccctgga actggtccca taggcggaga aaaaagcgtt tgtattgaat cgacgataag
    19321 caaaacagga ttgatactgg ccaaggcttc caaaacatct tccaagagcc ttgtgcatag
    19381 gacttcgata ttgtcttcaa agatagaaag cctatttgcg cggcttttta tttgacttgc
    19441 agattcttcc ccactcacat a
//
LOCUS       DAOSSW010000028        18130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_3236_length_18130_cov_6.372172, whole genome shotgun sequence.
ACCESSION   DAOSSW010000028 DAOSSW010000000
VERSION     DAOSSW010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 18130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18130
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3236_length_18130_cov_6.372172"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..457
                     /locus_tag="PKW26_03885"
                     /pseudo
     CDS             86..457
                     /locus_tag="PKW26_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(537..1340)
                     /locus_tag="PKW26_03890"
     CDS             complement(537..1340)
                     /locus_tag="PKW26_03890"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOQ92741.1"
                     /translation="MLLVLDIGNTNVVAALFYGDELVHEWRMYSDTKRTSDEYTSTLL
                     SLMRDAGVSQSSVKNAIISSVVPLLIGPFISLVDRITGKKPIIVSSALFPKLPIKVPE
                     TAAHEIGSDLVCNAVEAYATFQSPLIVVDFGTALTFTALDKNGYVQGIAITPGLRTAV
                     NSLFANTAQLPSVALEAPADSLGVNTIHAIQAGIVLGYKGLVESLIDQQKKDLASKTG
                     CSYEEIKVIATGGLNSVLSPITNIFSKIDKQLTLKGLCRIAKIVGIEAK"
     gene            1376..2683
                     /gene="hisS"
                     /locus_tag="PKW26_03895"
     CDS             1376..2683
                     /gene="hisS"
                     /locus_tag="PKW26_03895"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOQ92742.1"
                     /translation="MNTLIQAKVLKGFRDYLPNQEIKRALLIEKLTEAFRFYGFVPID
                     TPVLEYSEILLRKSEGETEKQVFRFEDNGGRDVALRFDLTVPFARFIASHKGELYFPF
                     KRYHIAKVWRGEKPQAGRYREFMQCDFDSIGSDSAESDFEILSLMKKSLSILGVENIT
                     IRLSHRGIFNAFLKKLNLHEKSEDVLRSVDKLAKVGRDEVISQLEAISGSKETAQAIL
                     RFISGDSDFDACLKLMEEMSGATREADRLRSLRAMMIAAGIEKSFVLDPSITRGLDYY
                     TGIVYETFLNDLPSIGSVCSGGRYDNLAGLYMKEEISGVGSSIGLDRLIAALEELGMG
                     EEKKSFVDAIIFYLDAPLALEYQALAEKLRRENIAVEVFPEPKKINQQYNLAEKKGIP
                     YGIIVKNEKDAKTGSYLLTLKNLETRESFESIDFEKALAILKK"
     gene            complement(2765..3133)
                     /locus_tag="PKW26_03900"
     CDS             complement(2765..3133)
                     /locus_tag="PKW26_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92743.1"
                     /translation="MKNKFLKSFFLFFFSLFLISSCKKNKAVLDVSVFQSITPTEQWA
                     VVVDSYVSFYADSSKKASVQANGRRGDVLKMQGEKWVRVLNGKEKWYFFEKGWLEQSS
                     IAIYSNALQAQKASQILLGD"
     gene            complement(3123..3530)
                     /locus_tag="PKW26_03905"
     CDS             complement(3123..3530)
                     /locus_tag="PKW26_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOQ92744.1"
                     /translation="MERKKSVAGIVYSDSLILIGKRLPTGSMASKWEFPGGKVEPDEN
                     CKEALIREFKEEFSIDIEVGEKIAEAEFTHKGVVSDLFAYRVYFLNENPTWVLSEHEK
                     IKWATIEEIKSLDFVDSDLLLIQQIEKKLAHEK"
     gene            complement(3546..3956)
                     /locus_tag="PKW26_03910"
     CDS             complement(3546..3956)
                     /locus_tag="PKW26_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92745.1"
                     /translation="MLQFYFFSILFNTLTGISLITLKKKNGEPLVEKPIELFENPTYK
                     LILGILTGFMGLMKLLSVVPNDVPIFGDFIPFVAGILGAAVLLFDYNTEKNAVDIAIP
                     SGVENLLVKGRVFVGYFCILAGVLHLLFPKVIFF"
     gene            complement(4032..4661)
                     /locus_tag="PKW26_03915"
     CDS             complement(4032..4661)
                     /locus_tag="PKW26_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92746.1"
                     /translation="MEDKNTKNADAFSQDFQSQGGFEKTDNAIDPSSLVFHYKRENRI
                     SKAPTLVKSSYDGTLKKPPKGFFRALVHTKSSRIMLIVMIVVLGFTVASILSGPKKHR
                     ANIDGIAFSLSAFSFEDTIYVSLQMQEDTNRAKKSIETKPEETVVQVDFSFIDADGNA
                     LVQQSFEKTFLNKEIFLRTTLSDYDILSVSCLLKIDEKEAMVESRVDRK"
     gene            complement(4678..5925)
                     /locus_tag="PKW26_03920"
     CDS             complement(4678..5925)
                     /locus_tag="PKW26_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92747.1"
                     /translation="MLYLYSQKIKKHGLFVLVLLIFSLCLSLLSCNRQQKLSSIEHEE
                     LFRLSYGSFEDEIDLFSLGQTGIIQSFIHMQDGFFYIANGEAKKVLQLTSFGDLLSII
                     YNTETNPSPSFIRQLDQGENLVATQHAVSYPFNKIGKLSVDFNKTLYITEELPLERQE
                     IDKENNVLLSQVVLVFSKDGNFVDYLGQEGPGGRPFPFITNIFTNKQNELIVSCLTTE
                     GQIVYWFSQEGFLKYTIPIYNDALPELESDTLLEASLDCIIPDYSSQKLYVKIDYYKN
                     YIDPETKAIAGIDFYNSAVSVLDIETGRYEEPILIPSYTEVVSQGYTKLNYSIPYNFL
                     GVTETGWLFFTIPDQSGFMIQMLQPNGQKILKNHFTLDLSEVLYHNFSLSQSGIISAL
                     LATEDAASVVWWRTDALTDSFIR"
     assembly_gap    6167..6266
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6372..9110
                     /gene="secA"
                     /locus_tag="PKW26_03925"
     CDS             6372..9110
                     /gene="secA"
                     /locus_tag="PKW26_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOQ92748.1"
                     /translation="MFSSIMKALFGSKTERDVKSLVPIVEKINAEEAWASALSKEAFL
                     ETTASFKKSIASGTKLEELLPKAYALSREASKRVLGERPYDVQLMGSIVLNSGKIVEM
                     KTGEGKTLMCVAAAYLNSLTGKGVHIVTVNDYLAERDAEWMRPVYEYLGVSVGIIVSN
                     LDNEARKKAYNCDITYGTNNEFGFDYLRDNMQVSVDQKVQRGFAFCIVDEIDSILIDE
                     ARTPLIISGQGEDDTARFFEVDKFVHQLVEAEKDPVTGDYPDEAQGQEIVGDYKIDEK
                     SKRVSFTDQGMLHIEEILQKNRLIEGSLFDEVNFEYIHYFTQAVRAHKLYKNDVDYVV
                     KEGQVQIVDEFTGRILEGRRYGDGLHQAIEAKEKIRIAQRNRTLATITFQNFFRMYEK
                     LSGMTGTADTEAVEFSKIYKLDVVVIPTNKPIARIDDNDEVYLNEHDKWEAIAKEIKE
                     CHAKGQPILVGTVSIEKSELLSALLTKKGIRHEVLNAKNHAREALIIAEAGAKGSVTI
                     ATNMAGRGTDIKLGGNAEFRARKKAGTEASAEVFAQVLAKEKESWKKDYEEVKAAGGL
                     YVIGTERHESRRIDNQLRGRSGRQGDPGRSKFFISMDDDLMRLFGGERMKTMMKRIGM
                     PDGEAIFHPWLTKGIEKAQTKVELRNFEIRKHLLDYDDVLNEQRNYIYAQRDAILNDD
                     NLRSRVMTTAEEVSEEYANTFKTMSRRDMATAFSHLQENLKDHFNFQLKSQDETSDVY
                     NAMLSYFERDLNYKEALAGKENLNMFIRYQYVQAIDRRWLDHLEQLEALREAVYLRSY
                     GSKNPLTEYKIDGFNIFYDMLDAIRLEIASRVFKVRVQTEDTSHRTSRKMQMNAQHNA
                     MQSFDGMQARQAAAASPMASKRQGDSITVVRSGPKVGRNDPCPCGSGKKYKQCCGR"
     gene            complement(9238..11334)
                     /gene="feoB"
                     /locus_tag="PKW26_03930"
     CDS             complement(9238..11334)
                     /gene="feoB"
                     /locus_tag="PKW26_03930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOQ92749.1"
                     /translation="MKLKIALIGNQNAGKTTLFNQLTGSRQHVGNFPGVTVEKKSGII
                     KKQYTHVFNTHARVGQPVTGEEVHNENLLQIPRNLEVELVDLPGIYSLSPYTPEERVT
                     RDFLIREKPDAIINILDATNIERNLYLSLQVMEMHIPMVIALNMMDEVRKAGNSIDID
                     RLSKDLHLDIVPIVANKTEGVSELVQRTISEAISKHKPQKIDFCTGPVHKAIHSVAHL
                     IENIAEKHSFPLRFASTKLVEGDEIIAEALHLGENDKMIIQNIVDDMEKALDTDREAA
                     LADMRYEFIAGVSQKSVHRKGDTIEQMRSVKIDRVLTHPIFAIPIFVGIMSLIFWVTF
                     GIIGASLSEWFEAFIEWITSIVDSGLTAFGLAAVPHSLIIDGAFAGIGSVLSFMPTIL
                     VLFFFLSLLEDSGYMARVAFVMDGLLRKLGLSGKSIVPMLIGFGCTVPAVMATRTLSS
                     RRDKLLTIFITPFMSCSAKLPVYALFTQVFFPKYAALVMIGLYIGGMVVAIISALLLR
                     NTFFRGKPVPFVMELPAYRMPTMKTMGLHIWEKARDFIQRAFTVIFMATLVIWFFQTF
                     DIGLNVVENSADSILAQLGSFMVPIFKPLGLDDWRLSTALVTGLTAKEAVVSTLAVLF
                     GSEAGLETALSSVLSVPSAMAFLVFILLYMPCFATLAVTKREIGTRGAIAAASFQTLT
                     AWIVALGVYRVFLLIF"
     gene            complement(11331..11741)
                     /locus_tag="PKW26_03935"
     CDS             complement(11331..11741)
                     /locus_tag="PKW26_03935"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOQ92750.1"
                     /translation="MRLSRLEPGKQAVIKSVAGEGALRRHLLDMGLTPNTEVFVRKVA
                     PMGDPIELCLRGYELTLRLSEAELISVEPKESFVFCSGSCSSCSSSHAHGYAADSGRG
                     RGGQGRGRGFFGRGGKGRGRGRGFSLFAKRGTEE"
     gene            12013..12233
                     /locus_tag="PKW26_03940"
                     /pseudo
     CDS             12013..12233
                     /locus_tag="PKW26_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707275.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12402..12830
                     /gene="rplM"
                     /locus_tag="PKW26_03945"
     CDS             12402..12830
                     /gene="rplM"
                     /locus_tag="PKW26_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOQ92751.1"
                     /translation="MKTIFVNEKDAVRNWYLIDASEKPLGRVAAKAALMLRGKQKTTF
                     TPNQEMGDYIVIINAEKVAVTGSKKTGKLYHHHTGYVGGLKTFTFEEKLERHPTEPLM
                     LAIKGMLPKGRLGRRMLTNVKVYAGNEHPHEAQNPQTLEV"
     gene            12851..13243
                     /gene="rpsI"
                     /locus_tag="PKW26_03950"
     CDS             12851..13243
                     /gene="rpsI"
                     /locus_tag="PKW26_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOQ92752.1"
                     /translation="MVKNIGIGTGRRKTAVARVYVRDGSGKVTINDKDIQDYFATAEQ
                     IQVVRQPLMVTASENKYDLVITVSGGGLNGQAGACLHGISRALSQVDQENYSALKANG
                     FLTRDSRMVERKKYGRPGARKRFQFSKR"
     gene            13209..13922
                     /locus_tag="PKW26_03955"
     CDS             13209..13922
                     /locus_tag="PKW26_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOQ92753.1"
                     /translation="MHESDSNLANVSILEEQLEDSKNTICIQGIKQVLSGLYKIHPTA
                     GPVFFVRDWYFGVEHFEKLLEEFETVFFADREKGEKTSFCLTEEQSLLLLDASEAYLA
                     ERYAMGLLNRSEQSRFLLFQKLLKKKFRISYINEVLDFLEHEGHLSDLRFSEAWLRQR
                     MRSKKESRTKLYQELLSRSISSQTAKRALDTAFEDVDEEEILRDLIDSYISKGFDRDK
                     IIKKCVYYGFPLKGINTLL"
     gene            13994..14605
                     /locus_tag="PKW26_03960"
     CDS             13994..14605
                     /locus_tag="PKW26_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ92754.1"
                     /translation="MAKDSQIHIVYSALEVAKICGVVNQTAINWIRGEHLKAFTTPGG
                     QFRVYLDDLIDFMRKRNMRIPQDVLDLYEQGSAKQASVLIVDDDKGLNSVIAKFLEKS
                     HEKIVVYQAFDGFEAGVQLVDKKPKCVLLDLDLPGVDGFEVCKKIYNSEEYGKPIVCV
                     ITALEDEDLEKEVKEMGAVEFFPKPLNLVEIANRIKKRLHLED"
     gene            complement(14658..15653)
                     /gene="sppA"
                     /locus_tag="PKW26_03965"
     CDS             complement(14658..15653)
                     /gene="sppA"
                     /locus_tag="PKW26_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOQ92755.1"
                     /translation="MTIKEQNPKTKKKNTGLIILGLIVVLGIIFAISQCNTGIKEIGM
                     QKSSSNRSAFWINLYKPESIAILEIVGTIEEANASYNQAWLLETLENLKNDKKNKALI
                     LEIDSPGGAVYQADELYLALKDYAKEKPLYAYFKSMAASGGYYIACAAEKIYANRNTL
                     TGSIGVISGMSLDITEMLDKLGVKMTTVHSGKNKNMLNLNEPFTEEQRAIMQSVSDEC
                     YEQFTGIVAEARNLDIALVKNLADGRIYTAKQALEAELIDGISLFEEALEELKSTYAF
                     DPDIHTERLSYQKKKSVWESMLDIKSFLPSRKTEAEIILQILEPPVAFPAYYYAP"
     gene            complement(15654..16382)
                     /locus_tag="PKW26_03970"
     CDS             complement(15654..16382)
                     /locus_tag="PKW26_03970"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOQ92756.1"
                     /translation="MINRVEIKTEAKTHLRGNWKYPILITLVTLVLSTLFNFRTSMSY
                     VTGIRLSATDEVFSFIWQFFAFIISVASAFFYLRFIREREAANFQTFLDGFNLFWKAT
                     LTFLLLMLRLIGWTLLLIIPGIIKAFAYSQVHFIIADYPDIKITDAFRLSNLMTRGYK
                     LDIFVFYLSFLGWILLTLITFGIANIFVSPYMDTAATKLYLHLKENAVQANLIDTNTD
                     EKESSEEINKEQKDEGEAKSETEV"
     gene            complement(16430..17275)
                     /locus_tag="PKW26_03975"
     CDS             complement(16430..17275)
                     /locus_tag="PKW26_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ92757.1"
                     /translation="MKIEKLNSELQLPKDAGLSFFFVGVGSAFSKTHFQTNIIITKNE
                     DHLLIDCGTLCPYALHSYKSNITKIQHFLITHSHADHIGGLEEAALMGRYVSKQKPTM
                     IISDEYKKILWSQSLRGGNSYGEISEGKYLNFDDYFTQIKPKLISKKPRPLYQVSCGS
                     IDLKLFRTKHIPDSVQSWRNSFYSLGVLVDERILYPSDTRFDTDMLYWMLEDYPTIEY
                     IFHDTQFYPGGVHAYYEDLKTLPNDIKKKMFLCHYGDNFSQYTPEDDGFAGFAKQAHF
                     YHFDR"
     gene            complement(17362..>18130)
                     /locus_tag="PKW26_03980"
     CDS             complement(17362..>18130)
                     /locus_tag="PKW26_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOQ92758.1"
                     /translation="KHLLPDFIQIGENSGYLKETFLPYFSPKNNEGNTENIAVLCAGP
                     DFTSALIGTNTLSPGLICDRAGSSEGFNLCTEWPLKAGTKLKHEDKTFDTEIRNLPSI
                     ISPFFNASTLIPDSGIRFSKTKRQSSFAKSSYTDYVEHLLENHDEAGYKIMIELAHEC
                     KLAFDILYDAYSDACQKQGTKKNIERKIIVTGGQAKNEAWLKLKAKIMEVSIQIPLCV
                     DAELVGNAAIAFFSLGIYSSIQTATQNISKIKKIISP"
BASE COUNT         5501 a         3446 c         3744 g         5339 t
ORIGIN      
        1 atgttgtcga tggcagtatg acctttggtg cctttgtttt ggttatcacc tactataata
       61 ttttgcagtc gatgaagcag aatatgaaag gctcgttcaa ttagtcgaac tagaaaaact
      121 aaaggagaga aatcttggaa gtggaggaga gcatatttca ggaggcgaaa aacagcgcct
      181 tgctctcgct cgttttttat atcaattgac agtaaaaaat aaagcctttt ttattataga
      241 tgaagctttt gtttctcttg atttttatac aaaagaaaaa atgcttgccc taacggcgga
      301 agcagtgaag ggtaaaacag gtatttgcat aacgcatgac gatgctgtct tccaggctct
      361 gggagataag gttttacttt gcaacaaaga aaataaattg gttctgaaag ataaaataaa
      421 ggggaaaaac ttaaaggatt atcttgacgc agaataatat gctttaagtt cagctttctt
      481 aaaaccccaa ggttgaccaa tagcctgcga caaagagcat gcgcaatact atttttctac
      541 ttcgcctcta ttcctacaat ttttgcgatg cggcacaaac ctttgagggt gagctgctta
      601 tcgatttttg aaaaaatatt tgtgatgggg cttaaaacag aatttaagcc gccagtggca
      661 attactttta tttcttcata gctacagcct gtttttgagg caagatcttt tttttgctgg
      721 tcgatgagcg actcgactaa acccttgtag ccgagcacaa tgccggcttg tattgcatgg
      781 atggtgttca cgccaaggga atcggcggga gcttcgaggg caacggaggg aagttgtgcg
      841 gtatttgcaa aaagcgaatt gactgcggtc cgtaatccag gagtgatggc tatcccttgg
      901 acatagccgt ttttatcgag agcggtgaag gtgagcgctg tcccaaagtc cacgacgatg
      961 aggggacttt gaaatgttgc gtaggcttct acagcgttgc agacgagatc gctaccaatc
     1021 tcgtgagcgg cagtttctgg aacctttatg ggaagctttg ggaaaagagc ggaggagaca
     1081 atgatgggtt ttttcccagt aatgcgatcg acgaggctta taaagggacc aatcaataaa
     1141 ggaacaaccg aagaaataat ggcatttttt accgaagatt gagagactcc cgcatcccgc
     1201 atgagggaaa gaagggtaga agtatattcg tcggaggtgc gttttgtgtc gctgtacatt
     1261 cgccactcat gaacgagctc atcaccataa aaaagagcgg cgaccacatt tgtatttcca
     1321 atatctaaaa ctaaaagcaa ttgtataaat ctcctttatt cgttattata ctcctatgaa
     1381 tacattgata caagcaaaag ttttaaaagg ttttcgagat tatctaccta atcaagaaat
     1441 aaagcgagcc ctactcatag aaaaactcac agaagccttt cgtttttatg gctttgttcc
     1501 tattgatact cctgttttag aatattcaga aattttactt cgaaaaagcg aaggtgaaac
     1561 agaaaaacaa gtctttcgtt ttgaggacaa tggtgggcgc gacgtagccc ttcgctttga
     1621 ccttacagtt ccctttgcgc gatttattgc aagccacaag ggcgaactct acttcccctt
     1681 caaacgttat cacattgcaa aggtatggag gggagaaaaa ccgcaagcag ggcgctatcg
     1741 tgaatttatg caatgcgatt tcgatagcat tggtagcgat tctgcggaat ctgactttga
     1801 aattctctct cttatgaaaa aatctctttc aattctcgga gttgaaaata taacaattcg
     1861 attaagccac cgcggcatct ttaatgcttt tttgaaaaaa ctcaatcttc atgaaaaaag
     1921 cgaagacgtt ttgcgctctg tggacaagct tgcaaaagtg ggtagagacg aagtgataag
     1981 tcaacttgaa gctatttctg gctcaaaaga aacagctcaa gctattttgc gttttatttc
     2041 gggagatagc gactttgatg cttgcctcaa gctcatggaa gaaatgtctg gtgcaacgag
     2101 ggaagccgac cgcctccgca gccttcgcgc catgatgatt gccgccggca tagaaaaaag
     2161 ctttgttctc gacccatcta ttacacgcgg tcttgattat tatactggca ttgtctacga
     2221 aactttttta aatgaccttc cctctatagg ctcagtctgc tccggcggtc gctacgacaa
     2281 tcttgcagga ctttatatga aggaagaaat ttctggcgtc ggctcatcca tcggtcttga
     2341 tcgactcatc gcagccctcg aagagctggg catgggagaa gagaaaaaaa gctttgtcga
     2401 tgcgattatt ttttatctcg atgcaccctt ggctttggag tatcaggctt tggcagaaaa
     2461 actacgcagg gaaaatatag cggtggaagt cttccctgag ccaaagaaaa taaaccaaca
     2521 gtataatctt gcagaaaaaa aaggcatacc ttatggcatc attgtcaaaa atgaaaaaga
     2581 cgcaaaaaca ggctcttatt tactaacgtt aaaaaaccta gagacgagag aaagttttga
     2641 gtccatagac tttgaaaaag cgcttgctat tctaaaaaaa taaaactaat cttttaagat
     2701 aaaaaaaggg gctgtctaaa aagtatttat atgatacttg cggcagtcct ttttttctgc
     2761 tggcttagtc gcctagtaaa atttgagagg ccttctgtgc ttgcaaggca ttgctgtaga
     2821 ttgcaatact cgattgttca agccaaccct tttcaaaaaa ataccatttt tctttgccat
     2881 ttagaactct aacccacttc tcaccctgca ttttcaaaac atcgcccctt cgaccatttg
     2941 cttgcacact tgcttttttg gaactatctg cataaaacga aacataggaa tcgacaacaa
     3001 cagcccattg ctcggtcggc gtaatacttt gaaatacaga aacatcgagt acggctttat
     3061 tttttttaca agaagagatg agaaaaagag agaaaaaaaa taaaaaaaag ctttttagaa
     3121 atttattttt catgtgccag ttttttttct atttgttgga tgagtagcaa atcagagtca
     3181 acaaagtcta agcttttaat ctcttcgatt gttgcccatt ttattttttc atgctcactt
     3241 aaaacccagg tgggattttc gttcaaaaaa tacacgcgat aggcaaaaag atcggataca
     3301 actcccttgt gagtaaactc cgcctcggca attttttccc ctacttctat atctatagaa
     3361 aattcttctt taaattcacg gataagtgct tctttgcagt tttcatcggg ctcgaccttt
     3421 ccccctggaa attcccactt gcttgccata gagcctgtgg gtaaacgctt cccaatgaga
     3481 atgagggaat cagaataaac aataccagct acagattttt ttcgctccat ttttataaat
     3541 acagcttaga agaaaataac ttttggaaat agaaggtgta aaacacccgc cagtatacaa
     3601 aaatagccca caaaaacacg tcccttgacg aggaggtttt cgactccaga gggaatggca
     3661 atgtcaacag catttttttc ggtattgtag tcgaataaaa gaacagctgc accaagaata
     3721 cctgcaacaa agggtataaa gtccccaaag atgggtacat cgtttggcac tacgctcaag
     3781 agtttcatca aacccataaa accagttaaa atacccaaaa taagcttgta ggttgggttt
     3841 tcaaacaatt caatcggttt ttcgacaagt ggctcaccgt ttttcttttt caaagtgatg
     3901 agactaatcc ctgttaaggt attgaataat atggaaaaaa agtaaaattg taacatactt
     3961 ctcctctaaa aatctttctt tgcaaaaacg caaagtatat atatagtagc aaaggcggtg
     4021 aaataagtcc gctactttcg gtctaccctt gattctacca ttgcttcttt ttcatctatc
     4081 tttaagaggc aactcaccga tagtatatca taatctgaaa gagttgtccg caaaaaaatt
     4141 tctttattta aaaaagtttt ttcaaagctt tgctgcacca aggcatttcc atctgcatcg
     4201 ataaaagaaa aatcgacttg cacgactgtt tcctcaggtt ttgtttctat tgattttttt
     4261 gccctgttag tatcttcttg catttgtaaa cttacataaa ttgtgtcctc aaaggaaaag
     4321 gccgagaggc taaaggcaat gccgtcgata ttcgccctgt gttttttggg accgctgagt
     4381 atacttgcga cggtaaaacc caaaacaaca atcataacga tgagcataat gcgagatgat
     4441 tttgtgtgaa cgagggcgcg gaaaaaaccc ttgggtggtt tttttagtgt tccgtcgtat
     4501 gaacttttta cgagagttgg cgccttggaa atacggtttt cccttttata atggaaaaca
     4561 agactcgatg ggtcgatagc attatctgtt ttttcaaacc caccttgaga ttgaaagtct
     4621 tgcgagaaag catcggcatt ttttgtgttt ttatcttcca taaaaaacct tcagcaccta
     4681 tctaataaaa gaatccgtca aagcatctgt tcgccaccat acgacagagg ctgcatcttc
     4741 agttgccaga agggcggata ttataccgct ttgagaaagt gaaaagttat gatacagcac
     4801 ttcgctcaga tcgagggtaa aatggttttt gagaattttt tgcccattgg gctgaagcat
     4861 ttgaatcata aaaccgcttt ggtctggaat agtaaaaaag agccaacccg tttctgtaac
     4921 gccgagaaaa ttgtagggaa tgctataatt gagctttgta taaccttgac tgacaacctc
     4981 agtataggaa gggatgagaa tcggctcctc gtaacgtcct gtttctatgt ctagaacact
     5041 caccgcggag ttgtaaaaat ctatccccgc aattgccttt gtctctgggt ctatataatt
     5101 tttatagtaa tcaattttta cataaagctt ttggcttgag tagtcgggaa taatgcagtc
     5161 taaagaagct tcgagcaagg tatcggactc aagctctggt agcgcatcat tgtaaatggg
     5221 aatagtgtat tttaaaaaac cctcctgcga aaaccaatac acaatttgcc cttcagttgt
     5281 aagacagctt acaatgagct cattttgttt atttgtaaaa atatttgtta taaaaggaaa
     5341 gggtcttcca ccaggacctt cttgtccaag ataatcgaca aagttaccgt ctttagaaaa
     5401 aaccaagaca acctgactca ataaaacatt gttttcctta tctatttctt gcctttccaa
     5461 gggaagctct tcagtaatat agagcgtttt attgaagtcg acagaaagtt tccctatttt
     5521 attaaatgga taagaaaccg catgttgtgt tgcaacaaga ttttcacctt ggtcaagttg
     5581 acgaataaaa gaaggacttg ggtttgtttc agtattgtag ataatgctta aaagatcacc
     5641 aaaagaggtg agctgtagca ctttttttgc ttccccgttc gcaatataaa aaaaaccgtc
     5701 ctgcatatgt ataaaacttt gtattattcc cgtttgtcca aggctaaaca agtctatttc
     5761 atcctcaaaa gaaccataag aaagtcgaaa aagctcctca tgttctatag aagaaagctt
     5821 ctgttgacga ttacatgaaa gaaggctgag acataaagaa aaaatcaata atactaaaac
     5881 aaagagacca tgttttttta ttttctgtga ataaagatag agcatgttag tagaataagc
     5941 ctcttagtag ctcttgtcaa cgggctttct cttacgtttc gcgactttac gagagctttt
     6001 aaaatcctaa tactcacttt cggaaagacc agtaaaacgt ttaatttgct caggaggaag
     6061 accttcagat ttcatactac gagcggtttg tctgagtttt tctaaacctt gttctaaacc
     6121 ttgttctaaa ccttgttcta aaccttgttc taaaccttgt tctaaannnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnncctt gttctaagcc ttctgcccga ccttcttcca
     6301 atgcttctgt tatgaggaca tttttttatg cccttcttgc tttttctcat gtttaggagt
     6361 acattgttta aatgtttagt tctattatga aagctttgtt tggttccaaa acagagcgtg
     6421 atgtaaaaag tcttgtaccc attgttgaaa agataaatgc cgaggaagct tgggcttcag
     6481 ctctttcaaa agaagctttt ttagaaacaa cagcgtcttt taaaaaaagt attgcctctg
     6541 gcacaaagct cgaagaatta cttcccaagg cctatgcgct ttctcgggaa gcttcaaagc
     6601 gtgttttagg cgagcgtccc tatgacgtcc agctcatggg ctccattgtg ctcaactctg
     6661 gaaaaatcgt cgaaatgaaa acaggtgagg gaaaaaccct catgtgtgtt gctgcggcct
     6721 acttaaacag ccttacagga aagggcgttc atattgtcac ggtaaacgat tatcttgcgg
     6781 agcgtgatgc cgaatggatg cgccccgttt acgaatacct tggtgtgagc gtcggtatta
     6841 ttgtttccaa tctcgataat gaagcacgaa aaaaagccta taactgcgat ataacctatg
     6901 gaacgaataa cgaattcggt tttgactatc ttcgcgacaa catgcaagta tccgttgacc
     6961 aaaaagtgca aagaggtttt gccttttgta ttgttgacga aattgactct attttaatcg
     7021 acgaggctcg tactccgctc attatttcag ggcagggtga ggacgatacc gcacgatttt
     7081 ttgaagtaga caagtttgta catcaattag ttgaagcaga aaaggaccct gtcacgggag
     7141 attatcctga tgaggcacaa gggcaagaaa ttgtcggcga ctacaagata gatgaaaaaa
     7201 gtaagcgggt ttcttttacc gatcaaggaa tgcttcatat agaagaaatt ttacaaaaaa
     7261 accgcctcat cgaagggagc ctctttgatg aggtgaattt tgaatatatc cactatttta
     7321 cgcaggcagt tcgtgcccat aagctatata aaaacgatgt tgactatgtg gtaaaagaag
     7381 gacaggtgca aatcgttgac gaatttaccg gacgtatcct cgaaggtcgg cgttatggcg
     7441 atggtttgca tcaagcaatt gaggcaaaag agaaaatccg cattgcccag cgaaatagaa
     7501 ctcttgcaac gattaccttt caaaactttt ttagaatgta cgaaaaactc tctggtatga
     7561 cgggaacagc tgataccgaa gcggttgaat tttccaagat ttacaaactc gacgttgttg
     7621 ttatcccgac aaataaaccc atcgctcgta tagacgataa tgacgaggtt tacttaaacg
     7681 aacacgataa atgggaagcg atagcaaaag agataaaaga atgccatgca aaggggcaac
     7741 ccatccttgt tggaaccgtt tctattgaaa aatctgagtt gctttctgcc ttattaacca
     7801 aaaaaggtat tcgtcatgaa gttttaaacg caaaaaacca tgctcgagaa gcgctcatca
     7861 ttgcagaagc tggtgcgaag ggctccgtta ccatagcaac aaatatggca ggacgtggta
     7921 ccgatattaa acttggtgga aatgcggagt ttagggcgag aaaaaaagcg ggcacagagg
     7981 cgagtgcaga agtttttgca caagtccttg caaaagaaaa agagtcctgg aaaaaagact
     8041 acgaagaagt aaaggctgca ggtgggcttt atgttatagg aacggagcgc catgaaagta
     8101 ggcgaattga caaccagctt cgcggacgct ctggacggca aggtgaccca ggtcgctcta
     8161 agttttttat ctccatggat gacgacctca tgcgcctctt tggtggtgag cgtatgaaaa
     8221 ccatgatgaa gcgaatcggc atgcccgatg gcgaggctat tttccatccc tggcttacaa
     8281 agggcataga aaaagcacaa acaaaggttg aactgcgtaa ctttgaaatt cgtaaacact
     8341 tgcttgacta cgatgatgtt ttaaacgagc agagaaatta tatctatgcc caaagagatg
     8401 ccatcttaaa cgacgataac ttacgctctc gtgttatgac aacggcggaa gaagtttcag
     8461 aagaatatgc aaacaccttc aaaacaatga gtcgccgaga tatggcaaca gccttttctc
     8521 acctacaaga aaacctcaaa gaccatttta attttcaatt aaaaagccaa gacgaaacaa
     8581 gcgatgtcta caatgcaatg ctttcgtatt ttgaaagaga cttaaactac aaagaagcgc
     8641 ttgccggtaa agaaaatctc aacatgttta ttcgctatca atatgtacaa gcaatcgata
     8701 ggcgatggct cgatcacctt gaacaattgg aagcacttcg agaagcggtg tatttgcgct
     8761 cttatggctc aaaaaatccg cttaccgagt acaaaataga tggtttcaat attttctacg
     8821 atatgctcga tgcaattcgc ctcgaaatag caagccgtgt tttcaaggta cgggtgcaaa
     8881 ccgaggatac cagccataga acctcacgaa agatgcagat gaatgcccag cataatgcaa
     8941 tgcagtcttt tgatggcatg caggctcgac aagctgctgc tgcgagcccc atggcttcaa
     9001 aacgacaagg cgatagcatt accgttgtgc gctccggccc caaggtgggt cgaaatgacc
     9061 cttgtccttg tgggagcggt aaaaaatata aacaatgctg cggaagataa aagcacgaac
     9121 agctttagca agacctgaga tgtcgcaagg tcttgcccca caaacagcat tgccctttca
     9181 aactaaagtt gtccttcaat ttctcaaaag ctttctagcc tgcaaatttc gcttgtgcta
     9241 aaagatcaat aagaacactc gatagacccc cagggctact atccaagcgg tgagcgtttg
     9301 aaagcttgcc gcagcgatgg cgcctcgtgt gccgatttct cgttttgtaa cagcaagggt
     9361 tgcaaaacaa ggcatgtata ataagataaa aaccaaaaaa gccatggcgc ttggaacact
     9421 gagcaccgat gaaagggcgg tttctaagcc tgcctcgctt ccaaagagca cggcaagggt
     9481 gctcacgact gcctcttttg cggttaagcc ggtaacaaga gcggtggaaa gtcgccagtc
     9541 atcgagacca agcggtttga agatgggcac cataaaagag cctaactgtg ccaagatact
     9601 atctgcagaa ttttccacga cattgagccc tatatcgaag gtttgaaaaa accaaatcac
     9661 caaggttgcc ataaagataa ctgtgaaagc tctttggata aaatcccttg ccttttccca
     9721 aatatgtaag cccattgttt tcattgtagg catacgatag gcaggcagtt ccattacaaa
     9781 gggaacgggt tttccccgaa aaaaggtatt tcgcaagaga agtgcagaga taatcgctac
     9841 gaccattccg ccgatataga gtccaatcat aacgagggca gcatattttg ggaaaaaaac
     9901 ttgcgtaaaa agggcgtaga cgggcagttt tgcgctacaa gacataaagg gtgtaataaa
     9961 tatcgtcaaa agtttatctc gacggctcga gagtgttcgt gtcgccataa cagcaggaac
    10021 cgtacaacca aatccgatga gcatgggaac aattgatttt ccagaaagcc cgagtttgcg
    10081 caagagtccg tccatgacaa aggcgacacg tgccatataa cccgaatctt ccagtaagga
    10141 gaggaagaaa aagagcacga ggatagtggg cataaaggaa agaacgctcc ctatccctgc
    10201 aaatgcccca tctatgatga gggaatgcgg caccgctgct aagccaaagg cagttagccc
    10261 cgaatcgaca atactcgtta tccattcgat aaaggcttcg aaccattcgc ttaagcttgc
    10321 tcctataatg ccaaaggtta cccaaaagat aagagacata atccctacaa aaataggtat
    10381 ggcaaagatg ggatgggtga gcactcggtc tatttttacc gagcgcattt gttctatcgt
    10441 gtcaccctta cgatgcaccg atttttggct tacccctgct ataaattcat agcgcatatc
    10501 cgcaagggct gcttctcggt cagtatcgag ggctttttcc atatcgtcta cgatattttg
    10561 tataatcatt ttatcatttt cgcctaaatg aagcgcttca gcgataattt catcgccctc
    10621 gacgagtttt gttgaagcaa agcgcaaggg aaaactatgt ttttcagcaa tattttctat
    10681 aagatgggct acggagtgta tcgccttgtg aacaggtccg gtacaaaagt ctattttttg
    10741 tggcttatgc tttgaaatag cctcagaaat agttctttgt acaagctcag aaacaccctc
    10801 ggttttattt gccacaatag gtacaatatc gagatgtaaa tcctttgata aacgatcgat
    10861 atcaattgag tttccagcct ttctcacctc gtccatcata ttgagtgcta ttaccatggg
    10921 aatgtgcatt tccataacct gcaaactcaa gtaaaggttt cgctcgatat ttgtggcatc
    10981 gaggatattg ataatagcat cgggtttttc gcgaattaaa aaatcccttg taacgcgctc
    11041 ttcgggcgtg tagggtgaga gcgaataaat accaggtaag tcgacaagct ctacttccag
    11101 atttcgcggt atttgcaaaa gattttcgtt atggacttct tcccccgtta ccggttgccc
    11161 aacacgtgca tgggtattga atacatgtgt gtattgcttt tttataatac ccgacttttt
    11221 ttcgaccgtt acaccgggaa aatttcccac atgttggcga cttccagtga gttgattaaa
    11281 aagagttgtc tttccggcat tttggtttcc tataagtgca atttttagct tcattcttca
    11341 gtacctcttt tagcaaataa agaaaaacct cttccacgac ctctgccttt tccaccacgt
    11401 ccaaaaaagc ctcgacctcg gccctgtcct ccgcgacctc tgccactatc tgccgcatat
    11461 ccatgggcat gactcgaact acaactcgag caagagccag agcaaaagac aaagctttct
    11521 tttggttcga ctgatattag ctcagcttct gaaagacgca atgttaattc atagcctcgt
    11581 aaacaaagct cgatggggtc tcccattgga gctacctttc gtacaaagac ttcagtgttt
    11641 ggcgttaagc ccatgtcgag aaggtgccgt cgtaaagcgc cttctcctgc aacgctttta
    11701 attacagcct gttttccagg ctccaatcgc gataatctca tatagtctgt ctcctaaagc
    11761 atctgcgctt attaagaaaa atattctagt actctatttt atacaaaaca gagaatgtta
    11821 acctaggcta acatttcctt tataatactc ttttcttatc gcttagtcaa ttgctaggga
    11881 atagaaaacg tatataaaga aagtcaataa atacatagac gatacacaaa ttttttgtag
    11941 ccttgctatg aaaaaaaaag ctagaaaaaa aagagagtat gcggtataat agaatcacta
    12001 gggggaattg taatggtttc aatgattatt gggtttattt ttatagcttt cactgtttta
    12061 gctgccttac cctttggcct tggctggggt agcgatatta ttgcctttct aaaggaggca
    12121 gccctgttct tgcagccttt atcggtctca ttgcaatctt tgtcggtatt gctgatattc
    12181 gcgataaaag agaagcaaaa aaagaagaag agcaatcacg cgcaaaagaa taaaaaagca
    12241 ttttctactt gactttttct cataaaaaaa agtatactat ctcaacccgt atatctgcgt
    12301 ttagactgtt agaaagtttg ctcactttta taacagataa accaaaaaag ctacaaagat
    12361 gctcactgta tgctttaaaa atgattttat aaggtaatgt aatgaaaaca atttttgtaa
    12421 atgaaaaaga tgcggtacgt aactggtacc tcatagacgc atcagaaaaa ccattgggaa
    12481 gggttgcggc aaaggcagcg cttatgctcc gtggaaaaca aaaaacaact tttacaccca
    12541 atcaagagat gggtgattat atagtgatta taaatgccga aaaggtagct gtaactggtt
    12601 ctaaaaaaac aggcaagttg tatcatcatc atacaggcta tgttggtggc ttaaaaactt
    12661 ttacctttga agaaaaactt gaaaggcatc ctactgaacc tctcatgctc gctattaagg
    12721 gaatgcttcc aaagggaaga cttggtcgca gaatgcttac caatgtgaaa gtgtatgctg
    12781 gaaatgaaca cccacatgaa gcacaaaatc cacaaacctt agaagtataa aggaaaagat
    12841 aggagaaatc atggttaaaa atattggtat tggtactgga agaagaaaaa cagctgttgc
    12901 acgagtatac gttcgcgacg ggtctggaaa agttactatc aacgacaaag atattcaaga
    12961 ttattttgca acagcagaac aaatacaagt ggtacgacaa cctttgatgg taactgcaag
    13021 tgaaaataaa tacgaccttg ttattaccgt ttctggcggt ggattaaacg gacaagctgg
    13081 tgcttgctta cacggcattt cacgcgcttt atcccaggtt gatcaagaaa actatagcgc
    13141 tttaaaggca aatggctttt taactcgtga ctcacgaatg gtcgaaagaa aaaaatacgg
    13201 tcgacctggt gcacgaaagc gattccaatt tagcaaacgt tagtatttta gaagagcaat
    13261 tggaagattc aaaaaacaca atttgcattc aaggtataaa acaggttctt tcagggttgt
    13321 ataaaataca tcccacagca ggacctgttt tttttgtgcg cgattggtat ttcggtgtag
    13381 agcattttga aaaacttctt gaagaatttg agactgtttt ttttgcagat agagaaaaag
    13441 gtgaaaaaac ttctttttgt ctaacggaag aacaaagcct gcttttactc gatgcaagtg
    13501 aagcctatct tgctgaacgc tatgctatgg gcttattaaa tagaagcgag caatcacgct
    13561 ttttactttt tcaaaaattg ctcaaaaaaa agtttcgcat ttcgtatatt aatgaggttt
    13621 tagatttttt agaacacgag gggcatttat cggacctgcg tttttcggaa gcttggttgc
    13681 gccaacgtat gcgaagtaaa aaagagagtc gcacaaagct ctatcaagag cttttgtcga
    13741 ggagtattag ctcccagacc gcaaaacggg ctctcgatac cgcttttgaa gacgtagatg
    13801 aagaagaaat tcttcgcgat ctgatagatt cgtatatttc caagggtttt gatagagata
    13861 agattataaa aaaatgtgta tattacggct tccctttaaa aggtataaat accctattat
    13921 aataaaaaaa aggcttactg aatttcaatt tttgtgttat gataattgaa gcgctaaggt
    13981 atagaggagc attatggcaa aagattcaca aatacatatc gtatattcag ctttggaagt
    14041 ggcaaagatt tgtggagtag taaatcaaac cgcaataaat tggatacgtg gtgaacactt
    14101 aaaagctttt acaactcccg gtggtcaatt tcgggtgtat ttagacgatt tgattgattt
    14161 tatgcgaaaa cgcaatatgc ggattcctca ggatgtattg gatttatacg aacaaggttc
    14221 tgcaaaacaa gcatcggtgc ttattgtcga tgatgacaag ggcttaaact ctgttatagc
    14281 aaaatttcta gaaaaatccc atgaaaagat tgttgtgtat caagcctttg acggctttga
    14341 agcaggtgtg caacttgtcg ataaaaaacc aaaatgtgtt ttgctcgatc ttgatttgcc
    14401 aggagttgat ggctttgaag tatgcaaaaa aatatataat tctgaagaat atggtaaacc
    14461 gattgtttgt gtaatcacag ccttggaaga tgaagattta gaaaaagaag tcaaagaaat
    14521 gggagcggtc gagtttttcc cgaaaccact caatttagta gaaatagcaa atcgtattaa
    14581 aaagcgcttg catttagaag attagctgtc ttcagctgaa aaaaaacagt caagaaaaaa
    14641 tcttgactgt tttttattta tggtgcgtaa taatatgcag ggaaagcgac aggcggttca
    14701 agtatttgta aaatgatttc agcttcagtt ttccgacttg gtaaaaaaga ttttatatcg
    14761 agcatagatt cccaaaccga ttttttcttt tgataggata gtcgttctgt atggatatct
    14821 ggatcaaaag cataggtcga ttttaattcc tcgagtgctt cttcgaaaag actaatgcca
    14881 tctatcaatt ctgcttcaag ggcttgtttt gcggtataaa tacgtccgtc ggcaaggttt
    14941 tttacaagag ctatatctaa gtttcgtgct tcagcgacga ttcctgtgaa ctgctcgtaa
    15001 cattcatctg atacgctttg catgattgcc ctttgttctt ctgtaaaggg ttcatttagg
    15061 ttgagcatgt ttttattctt tccagaatga acagttgtca tttttacacc aagcttgtca
    15121 agcatctcgg ttatatcgag ggacattccg ctgataacgc caattgagcc agtaagtgta
    15181 tttcggtttg cgtaaatttt ttctgcagca catgcaatgt aataaccgcc tgaagctgcc
    15241 atgcttttaa aataggcata gagaggtttt tcttttgcat agtctttgag agcgagatag
    15301 agttcatctg cttgatagac ggcgccccct ggagaatcta tttctagaat gagcgctttg
    15361 ttttttttat cgttttttag attttctaga gtttctaaaa gccaagcttg gttataactt
    15421 gcattcgctt cttctatggt gccgactatt tctagtattg ctattgattc tggtttgtaa
    15481 aggtttatcc aaaaagcact tcggtttgaa cttgattttt gcattcctat ttcttttatt
    15541 cccgtattgc attggcttat cgcaaaaata attccaagga ctacaataag ccccaagatt
    15601 ataagaccag tatttttctt ttttgttttt ggattttgtt cttttattgt catttatact
    15661 tccgtttcgc ttttcgcttc accttcgtct ttttgttctt tgtttatctc ttcgcttgat
    15721 tctttttcat ctgtgtttgt atcgataagg tttgcttgca cagcgttttc ttttaagtga
    15781 agatagagtt ttgttgctgc ggtatccata tagggtgaaa caaaaatatt tgctatgcca
    15841 aaagtgatta gagtaagaag tatccaaccg agaaacgaaa ggtagaaaac aaaaatatcg
    15901 agcttatagc cccttgtcat aagattgctc aggcgaaaag cgtcagtgat ttttatgtcg
    15961 ggatagtctg cgataataaa atgtacttgt gaatatgcaa aggctttaat gattcctggg
    16021 attatcaata gtagagtcca gccgataagc cgaagcataa gcagtaaaaa agtcagtgtt
    16081 gctttccaaa agaggttaaa gccgtctaag aaggtttgaa agtttgcagc ctctctttct
    16141 cgaataaagc gcaagtaaaa aaaagctgat gcaacagaaa tgataaaagc aaaaaattgc
    16201 catatgaaac tgaatacttc atcagttgca cttaggcgta tacctgttac ataagacata
    16261 cttgtcctaa agttaaataa ggtcgaaagt acgagcgtta caagagttat aaggattgga
    16321 tatttccaat tgccccgtaa gtgtgttttt gcttctgttt tgatttcaac acgattaatc
    16381 aattttttct ccttttcgat gaatacgatg ttcatcatgt tttatgtttt tatctatcaa
    16441 aatgataaaa atgtgcttgt tttgcaaagc cggcaaagcc gtcgtcttct ggagtatact
    16501 gagaaaaatt gtctccataa tgacagagaa acattttttt ctttatgtca tttggaagcg
    16561 ttttcaggtc ttcataatag gcgtgcactc ctccaggata aaactgggtg tcatgaaaaa
    16621 tatactctat cgtgggatag tcttcgagca tccagtagag catatcggtg tcaaaacggg
    16681 tatcagaagg gtagagaatg cgctcatcga cgagaactcc aagcgaataa aaagaattgc
    16741 gccatgattg aacggaatct ggtatatgtt ttgtgcgaaa aagctttaag tctatagaac
    16801 cgcagcttac ttgatatagt ggacgaggtt tttttgaaat aagcttgggt tttatttggg
    16861 taaaataatc gtcaaagttg aggtattttc cctcagagat ttctccatag gaatttcccc
    16921 cacgtaaaga ttggctccag agaatttttt tatactcatc tgaaataatc atagtcggtt
    16981 tttgctttga aacatagcgc cccataagtg ccgcttcttc taagcctcct atatgatccg
    17041 catgggagtg tgttattaaa aaatgctgta tttttgtgat atttgactta taactgtgga
    17101 gtgcgtaggg gcaaagagta ccgcaatcga ttaagaggtg gtcctcattt tttgttatga
    17161 taatgtttgt ttgaaaatgc gtttttgaaa aagcacttcc aacgccgaca aaaaagaatg
    17221 ataaacctgc atcttttggt aattgtaatt cactattaag tttttctatt ttcatacatc
    17281 cctcaataca gtatagtata acacagattt tttaataaat ctgcactttt tttttatttt
    17341 ttattagtaa gattcagtgt tttaaggaga aattattttt tttattttcg aaatattttg
    17401 tgttgcagtt tggatacttg aataaatgcc gagagaaaag aaggcaatag ccgcatttcc
    17461 gacaagttct gcatctacac aaaggggaat ctgtatgcta acttccataa tttttgcttt
    17521 tagtttgagc caggcttcat tttttgcctg ccctcctgta acaattattt ttcgctcaat
    17581 gttttttttg gttccctgct tttggcaggc atcgctatag gcatcataga gaatatcaaa
    17641 ggcaagtttg cattcatggg caagttctat cattatttta tatccagcct cgtcatgatt
    17701 ttctagaaga tgttctacat agtcggtgta ggagcttttc gcaaagcttg attgccgttt
    17761 tgtcttgcta aagcgaatac cgctgtcggg tatgagggtg cttgcattaa aaaaagggct
    17821 tataatagac ggaagattgc gaatttctgt atcgaatgtt ttatcttcat gttttagctt
    17881 ggtgccggct ttgagaggcc attctgtgca gagattaaaa ccctcactgg aacctgctct
    17941 gtcacatatg agtccggggc ttagggtatt ggttcctatg agtgctgatg taaaatcggg
    18001 acctgcacaa agaaccgcta tgttttctgt gtttccttca ttgttttttg gggaaaaata
    18061 cggcaaaaaa gtctctttta agtagcctga gttttcacca atctgtataa aatcgggtag
    18121 taagtgtttt
//
LOCUS       DAOSSW010000029        17772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_3309_length_17772_cov_5.666591, whole genome shotgun sequence.
ACCESSION   DAOSSW010000029 DAOSSW010000000
VERSION     DAOSSW010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 17772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17772
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3309_length_17772_cov_5.666591"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /locus_tag="PKW26_03985"
     CDS             complement(<1..1696)
                     /locus_tag="PKW26_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92759.1"
                     /translation="MAVQDYDAALILLQEYIAKKPQDFDAAQKRLKKIINSRIAFSEK
                     AEELVGVLMNEPLEDKKKLDMIASLEALLEKNPSTLYTGFIQETKAAAQFTYYRAVFD
                     EIMEKGSLLVEKGAYNEAIQLFYSGLDLYQKEFFEENWDPALKQEVKNKLELLPILIA
                     DFPQILANLDEAEIVFSEKQNNLATVLDFFPVFLDSFQKFASYQNEIQSIGAFFNNSF
                     IDLQKENPNLTEASFLAFASRFLLGRQNSETTGIIASYNQQWNKKIEPFLIASDLLIQ
                     KEFNTSSLMIQSLKTNFNLTSLEKAKTSFAETEKALNYVENLLALYQLKRENDGSASV
                     YHDTSRSKNLLFLKALKAEYVLFASNIEKEANEKNLFFAFKESIIASEYASNLFKNTK
                     EILDLNQDYNRAEERIRGLNDTTYEVWLVFYNTLLRDLEGSIQENLAYLSQEWDSFAH
                     FLENEGKLIENHYANLYAEGLETLNPEKKENPETLLALSYPAESILLFNEILKNIDAD
                     TREIDEKNKSLLESKVFHESLFTKEVEKSSSFFVKLISDLENLKRQTQSRILIAEQE"
     gene            1930..3228
                     /locus_tag="PKW26_03990"
     CDS             1930..3228
                     /locus_tag="PKW26_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HOQ92760.1"
                     /translation="MRKNKFLLITFILFSLIKSLASLEYSDVYADVSEFFSVFIDPNE
                     GLTSFRSLLIPSGGRPEALGNAYTGMANDVSYIEYNPAASAVLDETEIGVFHNAWILD
                     TNVESFVFTSRKRNLGWGAQIKALYLPFTEYDLFGNSVSAGYYSETIAAINLAYNFFA
                     GYTFKGLAVGTSLKTAFRSVPDYVNAETDQIDKNAGLGQSGLAFMADLGFLFRFNAFK
                     FYSSREANMQVGLSFMHFGAGITGFAQADGVFLDDPLPSKINLGFSYKPIRPLTFVAD
                     ISQPLNLQSIGESGLLSFGGGMALQITPFFALHSGFFLSGGNPRFSLGSEFMLFSYLA
                     NVNYTLDLTSSINPISRFSLALKMNLGDKGRKSDAEKVDELYVKGLEAYYLLHLEEAI
                     RLWEEALEIDPGFDPAIIAIKAAQATIDIQEKIRDIQKLE"
     gene            complement(3259..4845)
                     /locus_tag="PKW26_03995"
     CDS             complement(3259..4845)
                     /locus_tag="PKW26_03995"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOQ92761.1"
                     /translation="MKKKSFALHLLLHQGYFRDINNSESDKNQLLFYAISQTYLPLLN
                     MFANLESDGINFKLGLTITPSLCTLLTDPVVQQSYIEWLDKLILLGEAEIKKHEGNPE
                     TQELAKEYLRTCKRNKRDFVETFNQDILSKFAYYAHKGNIELFASAATNCFLPHYIDL
                     KEAIHAQVEAGLIAHKSFFNLAPEGFWLPNLGYTPGLEHILRSYGLNYAIIETHGLLF
                     STPPSKNGIFSPVRSENSFAFFGRDPYAEKEIMGDEGYIHNEIYRNEENDIGFESDIE
                     ELTAILAQNKERVSTGYKYFSKASENIKKKQYDIEKARQQADKDAQDFLAKKIDLLHK
                     AAELSEEDEVSLVATFDASLFGEKWFEGIDWLERFFRHASKQEEINIENCSDLAKNQF
                     TLQVVQPYHSAASGFGYGEDLVDNSNEWMVRYVHKSTERMIDLAQRFTDDTGLKARAL
                     NLAAKEILLAQASDWPSMLHNKNYPDFAREAFSQNVSSFATVFESLGSNNTSTEWLTN
                     TERAHQIFPWINYRIFSKKK"
     gene            complement(4869..5525)
                     /locus_tag="PKW26_04000"
     CDS             complement(4869..5525)
                     /locus_tag="PKW26_04000"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HOQ92762.1"
                     /translation="MRNKIITKADLESMSTSALVSLADEYNIEIPESLNRRFIISELL
                     EIAEEQSKTHQDDLRDDASAFTFYEDLPKNFNETYIGAIMRNPLWCFAYWDIRDSEFK
                     EISQNPNYLGLTLSLVFYKDGVEVKKEGIEIEVHLEKKEQFILLNPAYNSFRLHLIAE
                     FSNTEPKIISSTQKITIPHIILEKNEPGIDKKVSPIIALSGMHDILREHYNNHRQTFI
                     "
     gene            complement(5619..6266)
                     /locus_tag="PKW26_04005"
     CDS             complement(5619..6266)
                     /locus_tag="PKW26_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOQ92763.1"
                     /translation="MEDNILTIEEVAKYLRVSERTVYDWAQKGEIPSGKIGTVWRFKK
                     TEIEKWVNDRLSTGSRTSPFGAQIQIKNILSPDRVIFLNHSSKQDALVALADNLSTAP
                     QVKNAQELVSEILKREELMSTAIGRGIAIPHVRLSSVTDLIMSVGISKVDVQDFQTID
                     DTPVRLLFMIAASYNQHAYYLQTLSYFSTKLKNKDLREALLETENLLDAYNLLVQ"
     gene            6417..7508
                     /locus_tag="PKW26_04010"
     CDS             6417..7508
                     /locus_tag="PKW26_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92764.1"
                     /translation="MKIKEKKWFFSLLGFLIVLLYVLFSSQPLKKELQLLPEWTVNLE
                     DSYLAHTNEDKLFAQSETEVLLPFLQGQYLGAVYPSGEIAFIEAYAQRASLSKKYWIP
                     YEMNAKKTLVHPMDGGEPFLIKEEGFPFAIDSFLFTFFPGGNSFGSYDSEGQLLWSAS
                     QWSPISAFSVSDKAVLVGYIDGNLRLFSADGDLLFSSYPAGSKYEIIYGAALSSDSQL
                     LASICGLEKQRFVLYTVDGENTKIIHHEYIDGEKTRARFMQFNADMSKVFYDVDQGLA
                     AFDIKTKTTNLVPLTGNICSMQNLSFENTIVVLSKKNNSWYLSLLDAYDEIIASFSFT
                     AESASLFTYENALFLGRDASLSKMELVRK"
     gene            7505..>8289
                     /locus_tag="PKW26_04015"
     CDS             7505..>8289
                     /locus_tag="PKW26_04015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ92765.1"
                     /translation="MKKVVKVQKIRLSVFILSLMLGTVFFSQMPMQAFEWPQEIASLN
                     EILFFFGEDRKDFISSSLVFPKGEVIKLSEAGKVLFQYEAEQSCSRFPSSLGNALVLA
                     HENDLRSLYANLGEIAVEKDADFLQEGLEIAMSGDSGWHEDGAGLEFAVIDVKNKALV
                     NPLLVFPMGYKAIDFAIGKIYIKDVKGDYRELGTGKSFEEGFYTFYRESRDKMPLFRS
                     LVSLNGKTIDSLSYDSLSLWKEKLVLKSSQAVLLDYKQLYPME"
     assembly_gap    8290..8389
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8429..9028)
                     /locus_tag="PKW26_04020"
     CDS             complement(8429..9028)
                     /locus_tag="PKW26_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOQ92766.1"
                     /translation="MQEWLYSEVVKEHFTDPKNVLMENEDQWPCDGRGIVGNIRCGDQ
                     MLILLRVKDDVISDVRWKTYGCASAIASTSILSEAIKGMHIEEAYKLKPQDIVAKLGG
                     LPSNKVHCSVLGDKALRAAINDYLEKQGRASLEGKEEVTVICSCLNITDKDLEEAYKA
                     GDRSWADLQARTKIGTVCGGCKEEAMEKMHEFDHLYGED"
     gene            complement(9065..10225)
                     /locus_tag="PKW26_04025"
     CDS             complement(9065..10225)
                     /locus_tag="PKW26_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOQ92767.1"
                     /translation="MHKRIYLDYNATTPLSEEVKHFYISELEKFGNASSLHEDGRLES
                     KKIEKARVEFAELVNCSANEIIFTSGGSESNNTVFQTMISLSKKMQRNTIVISAIEHP
                     CVIESAKELEREGFRLIEVAVNPDGRINMDEYQKALSENPLLVSIMAANNEIGTVQDI
                     QVLADLAHQSGALFHSDAVQAVGKIPVDLKAWNVDYATFSSHKIYGPKGIGALYVKEG
                     RNIEPLIRGGHQEKGFRAGTYNAPAIAAFGLAASLAKKELSSYKSHTWKLREALREGF
                     LEKIPNIHVNGHESEVLPNTLNVSFPGAEGEAILLHLDLLGISVSTGSACASGSLDPS
                     HVLMAIGLGPELAHGSIRFSFGKYSTMEEVDYILEKFPPVIDRLRKMSSIPM"
     gene            complement(10218..11444)
                     /gene="mnmA"
                     /locus_tag="PKW26_04030"
     CDS             complement(10218..11444)
                     /gene="mnmA"
                     /locus_tag="PKW26_04030"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOQ92768.1"
                     /translation="MTYESTGLSKKSPIAIQEVSSPKAGSTVVVALSGGVDSALTALL
                     LKERGCTVIGATMSLWDEGYPIPHSESGRRESCYSPDEEKDIAECRSFCKEHDIPYYL
                     IPVAKVYNEKVLDYFKAEYRSGKTPNPCIQCNRFVKFGAFIEGIQKELDAKGIGFDYF
                     CTGHYARIVQIKDDIASVYGEDAKTGLHPFVVTQAKDGEKDQSYFLCRLPSSILEKVR
                     FPLSDFTKKEVYEMAKKRELAASKRKESQDFVPQTYFDILFQDKKAQEGSFINPEGRI
                     LGKHRGLEHYTVGQRRGLGISDVKPLYVQAIDSQNNTIILAYEDDLFSKALLSENWVW
                     AGDYRPEKSFRAWVKIRLASKPVEADIIPSIEDTSVLVQFLEAQRAVAPGQSVVIYKE
                     GLSLGGGIISRVIENA"
     gene            11568..12002
                     /locus_tag="PKW26_04035"
     CDS             11568..12002
                     /locus_tag="PKW26_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOQ92769.1"
                     /translation="MKISTRGRYALRLLLDLMLQDKDSYVSLKEISSRQDISVKYLEQ
                     ITSLLTKAGLLISTRGPTGGYKLAKSPDHIRVYDVMVATEGPLHSVACLGETENPCPR
                     KDSCTTLGVWEGFDKVVYDYLTSITLSDALTLEDKEAVNFSI"
     gene            12100..13524
                     /gene="amrA"
                     /locus_tag="PKW26_04040"
     CDS             12100..13524
                     /gene="amrA"
                     /locus_tag="PKW26_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOQ92770.1"
                     /translation="MNRKAILGAAVLPHPPLAVGKVAIDKKEIIKKTIASFHSLAKEI
                     ERLAPQTIVLISPHTNLYADYFHLGSYNEGEKIAWGDFSRFGDAESRVEVGFDEPFID
                     ALAELCQKEDFPCGKLGKKTSFLDHGITVPLYFVQKQYSDFQLVQVGLSGLGLAEHYA
                     LGMKITEIAEKLGRNTYIIASGDLSHKLLADGPYGYAAEGPEYDERIMAVLERAAFGE
                     MLDFDEAFLERAAECGHRSFCIMAGAFDGLKVDAKKLSYEGPFGVGYGAALFIPIEKD
                     TARHFLEGFREKEAKKLTERRKNEDPYVQLARASIEHFVNCGKVLELDAKLKEKIVLE
                     KEAYTKKAGCFVSLKKNGQLRGCIGTISPTQKNIAEEIIANAISSASRDGRFEPVRVD
                     ELSSLVYSVDILSESEAIASEKELDVKRYGVIVSAGHKRGLLLPNLEGVDSIDMQVSI
                     AKQKAGILPHEKVSLERFEVLRYE"
     gene            13517..14404
                     /gene="amrS"
                     /locus_tag="PKW26_04045"
     CDS             13517..14404
                     /gene="amrS"
                     /locus_tag="PKW26_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOQ92771.1"
                     /translation="MNKKHLVNCDICHHRCKIALGAYGFCGIRQNLDGKLVNQGYGKI
                     TALALDPIEKKPLYRFYPGSAILSVGSLGCNMNCSFCQNHHIARLRLDSAESNSAWES
                     YTPKALVELAIETKKQGNRGLAFTYNEPLINYEFIIDCAKKMKEMDSELKVVLVTNGH
                     LEIAHAKKLLPYVDAANIDLKAFRKAFYTKMGGDFSLVKAFIEEAVKNCHVELCYLLI
                     PGLNDGLDEVEEAAEWIASLDCTGQGPLENGKDIALHISRFFPTYKLVDRAATSVSAV
                     YEAVAVVKKHLNWVYPGNV"
     gene            14584..14799
                     /locus_tag="PKW26_04050"
     CDS             14584..14799
                     /locus_tag="PKW26_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOQ92772.1"
                     /translation="MPKGKPIIDIEKCKGCALCVTACPKKIINMSSQTNAQGVHFAYC
                     VDETKCIACMSCATICPDMVIEILKFD"
     gene            14814..15869
                     /locus_tag="PKW26_04055"
     CDS             14814..15869
                     /locus_tag="PKW26_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOQ92773.1"
                     /translation="MNDKILMKGNEAIGEAAILAGCRHYFAYPITPQNEIPAYMALRL
                     PEVEGTFLQAESEVAAINMVFGASAAGARVMTSSSSPGIALKQEGFSYLAGAELPAVV
                     VNMMRGGPGLGNISGSQGDYHQATRGGGNGDYKTIVLAPGTVQELADLTIKAFELADK
                     YRMVAMILGDGYLGQMSEPLTLPKAVQNIPSKADWALTGPKGRAGRSIASLRLAPDDA
                     LYQHNMHLAEKYAQVEAAETMAESYLCDDADIVLCAYGTSARICRKAIKKLRSEGIKV
                     GLFRPISLWPFPKKELAKVASGAKKLLTVEMSLGQMLDDIRLHSGKIPSEVDFYGTPG
                     GALPKIETIIEKVRSYV"
     gene            15862..16671
                     /locus_tag="PKW26_04060"
     CDS             15862..16671
                     /locus_tag="PKW26_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOQ92774.1"
                     /translation="MSKLIYTKPEALRPVKTHYCPGCGHGIVHKLIAQIIDELGIRED
                     TLLVAPVGCAVLAYNYIDVDGLESAHGRAPAISTGLKRVHKDKIVICYQGDGDLLAIG
                     AAETIHAANRGEDITVIFINNAIYGMTGGQMAPTSLEGQVTKTTPKGRDASEVGYPIR
                     ACEMLNTLVAPVYIERCAVYDVPHINKTKAAIRKALRYQKEGKGYSFVEILSMCPTNW
                     GVDPVKGAEWVKNAMESYYPLAVFRDIPAGKVPELAAPEGTSAYERREEAR"
     gene            16668..17219
                     /locus_tag="PKW26_04065"
     CDS             16668..17219
                     /locus_tag="PKW26_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOQ92775.1"
                     /translation="MTKEIIFAGFGGQGVILAGKVLCLACMSDGKSVSHIPAYGAEMR
                     GGTANCSVIISDEDIASPVIDKSDIVIALNKPSMVKFEKNLKENGLLIYNTSLIDLKP
                     SRTDIKTLGIPVNAMAEESGNPRGINMVVLGVLAKLFPEMLSKHAHFTAALDEAISER
                     NKKFNPLNIATIESAYAKDYVLE"
     gene            17231..>17772
                     /locus_tag="PKW26_04070"
     CDS             17231..>17772
                     /locus_tag="PKW26_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOQ92776.1"
                     /translation="MENKNSLGKKVQLFREMRNLSLEEVSKRSGLCIDEIEKIEKGDL
                     IPSLTPLIKIARVLGTRLGSFLDDDENLGPVVTRKETHTSITRFSNRDGAVSSDLDFF
                     SLAQNKTSRHMEPFLIDIHPLSEKEYRMSSHEGEECIYVLSGSIEVKYGSETHVLNEG
                     DSIYYDSIVEHHVHSASGEAA"
BASE COUNT         5085 a         3422 c         3608 g         5557 t
ORIGIN      
        1 tctcttgctc tgcaatgaga atacggcttt gcgtttgtct ttttagattt tccaaatcgg
       61 aaatgagctt gacaaaaaag gagctgcttt tctcaacttc ttttgtaaat agactttcat
      121 gaaaaacctt agactctaaa aggcttttgt ttttttcatc aatctccctc gtatcggcat
      181 caatattctt gagtatttca ttgaaaagta aaatactttc ggcaggatag cttagcgcaa
      241 gaagcgtttc tgggttttcc tttttttcag gatttaatgt ttctaggcct tctgcgtata
      301 gatttgcata atgattttct attagttttc cttcattttc taaaaagtga gcaaaactat
      361 cccactcttg acttaaatac gcaagatttt cttgtatact gccttccaaa tcccttagca
      421 aggtattgta aaagacaagc catacttcat atgttgtatc atttaaccct ctgattcttt
      481 cttcagcacg attatagtct tgattcagat cgagtatttc ctttgtgttt ttgaaaagat
      541 ttgaagcata ttcagaagct atgatagatt ccttaaaagc aaaaaagaga tttttctcat
      601 tagcttcttt ttcaatatta cttgcaaata aaacatattc agcctttaat gcttttaaga
      661 ataaaagatt tttactgcgg cttgtatcat gatatactga agcagaaccg tcattttctc
      721 tttttaattg atatagggcc aagagatttt ctacatagtt cagagctttt tctgtttcag
      781 caaagcttgt ttttgctttt tctagactcg ttaagttgaa atttgttttg agactttgaa
      841 tcattaggct tgatgtgtta aattcttttt gaatgagtag atccgatgcg attaaaaatg
      901 gttctatttt tttattccat tgttgattat atgaggcgat aattcctgtt gtttcgctgt
      961 tttgtcttcc caaaagaaat cgactcgcaa atgctaaaaa agaagcttcg gtaagatttg
     1021 gattttcttt ttgtaagtct atgaaactat tgttaaagaa tgctcctatg ctttgtattt
     1081 cattttgata tgaggcaaat ttttggaaac tgtccagaaa aacagggaaa aagtctaaaa
     1141 cagtagcgag gttattttgt ttttctgaaa aaactatttc tgcttcgtct aggtttgcaa
     1201 gaatctgcgg aaaatctgca atgagaatcg gtaagagctc gagtttgttt ttcacctctt
     1261 gttttaaggc tggatcccaa ttttcttcaa aaaattcttt ttgatataaa tcaagtcctg
     1321 agtagaatag ttgtattgct tcattataag cccctttttc aacaagaagg ctcccttttt
     1381 ccatgatttc gtcaaaaaca gcacggtagt aggtaaactg tgcagcagcc tttgtttctt
     1441 gtataaaacc ggtatataaa gtgcttgggt ttttttcaag cagtgcttca agacttgcaa
     1501 tcatatcgag ctttttttta tcttcaagcg gttcattcat aagaactcct acgagctctt
     1561 cagctttttc agaaaaagcg atacgagagt tgataatttt tttcaatctt ttttgagccg
     1621 cgtcaaagtc ttgaggtttt tttgcaatgt attcttgcaa aagaataagt gctgcatcgt
     1681 agtcttgtac agccatcaat tcatcgatag ttttaaaagg agactccttt ttaccagcgg
     1741 caaaaagagt gaaaagagaa aaaaataaaa tacaaatgaa aaaggctttt tttatttccc
     1801 aaccccttat tacaacattt aatagaaaaa aaacactatt ctctattagt tttcggcatt
     1861 ttaaagccga ttatgtagta tcttagtctt ttttgcgata taattacata gggtggaagg
     1921 tggaatttga tgcgaaaaaa caagtttttg ctaataacat ttattttatt ctcgttaatt
     1981 aaatctctag caagtttaga atattcggat gtgtatgctg atgtttcaga gtttttttca
     2041 gtttttatcg atccaaatga agggctcaca agctttcgta gccttttaat tccctctgga
     2101 ggaagacccg aagcacttgg caatgcatat acgggcatgg caaacgatgt aagttatatc
     2161 gaatacaatc ctgcagcaag tgcggtgctc gatgagacgg aaatcggtgt ctttcataat
     2221 gcctggattc ttgacaccaa cgtagaaagc tttgttttta cgagccgaaa aagaaattta
     2281 ggctggggcg ctcaaataaa agcactctat ctaccattta ccgagtatga tttatttgga
     2341 aactccgttt ctgcaggcta ttatagtgaa acaattgcag ccatcaatct tgcatacaat
     2401 ttttttgcag gctatacctt caaaggtctc gccgttggaa caagtcttaa aacagccttt
     2461 cgcagcgttc ccgattatgt aaatgcagaa acagatcaaa tagataaaaa tgcaggttta
     2521 gggcaatcgg gcttggcatt tatggctgac cttggttttc tttttcgctt taatgccttt
     2581 aagttttata gttctcgtga agcaaatatg caggtggggc tttcttttat gcattttggt
     2641 gcaggtatta cagggtttgc acaagcagac ggtgtttttt tagatgaccc actcccttca
     2701 aagataaatc ttggtttttc gtataaacct attcgaccgc ttacctttgt agcagatatt
     2761 tcgcaaccct tgaacttaca atccatcggt gaatcaggtt tattgtcttt tggcggcggt
     2821 atggccctgc aaataacacc ttttttcgca ctccattcag gttttttctt gtcgggggga
     2881 aatccacgtt ttagcctcgg ctctgaattt atgctctttt cctatcttgc caatgtgaat
     2941 tatacccttg atttgacttc gagtataaat ccgataagcc gttttagtct cgccttaaaa
     3001 atgaatttag gcgataaggg acgaaagtca gatgcagaaa aagtcgatga actctacgtg
     3061 aagggcttgg aagcatatta tctattgcat ttggaagaag ctattcggct ttgggaagaa
     3121 gccttggaaa tagacccggg ctttgaccca gcaattattg ctataaaagc ggcacaggcg
     3181 acaatagata ttcaagaaaa aatccgtgat attcaaaaac ttgagtagct tactttttat
     3241 ctggcttttt gagaaaaact attttttctt actaaaaata cgataattta tccagggaaa
     3301 aatttggtgt gctcgctctg tatttgtaag ccattcggta ctcgtgttgt ttgaaccgag
     3361 ggattcaaaa acagttgcaa aggagcttac attttgcgaa aaggcttctc gagcaaagtc
     3421 tggatagttt ttgttgtgaa gcatgcttgg ccagtcactt gcttgtgcta aaagtatttc
     3481 ctttgcagca aggttgagtg cacgggcttt taagccggtg tcatcggtaa agcgctgagc
     3541 gaggtcaatc attctttctg tcgacttatg aacatagcgc accatccact cattcgagtt
     3601 gtcaactaag tcctcgccat agccaaaacc agacgctgca gaatgatagg gctgtacaac
     3661 ttgcagggta aattgattct ttgccaaatc gctacaattt tctatgttta tttcttcttg
     3721 tttacttgca tgcctaaaaa aacgttcaag ccaatcaatg ccttcaaacc atttttcacc
     3781 aaagagggaa gcatcaaagg tcgcaacgag ggaaacttca tcttcctcgc ttaattctgc
     3841 agccttatga agtaagtcta ttttttttgc caaaaaatct tgtgcatctt tgtcagcttg
     3901 ttgtcgtgct ttttcaatgt catattgttt tttttttatg ttttctgaag cttttgaaaa
     3961 atatttatag cccgttgata cacgttcttt attttgtgct aaaatagccg ttagttcttc
     4021 tatgtctgat tcaaagccta tgtcattttc ttcatttcgg tatatttcat tgtgtatata
     4081 tccttcatcc cccattattt ctttttctgc ataggggtct ctaccaaaga atgcaaaaga
     4141 attttcactt cgtactgggg aaaagatacc gtttttgctc ggtggcgttg aaaataaaag
     4201 cccatgtgtt tcaataatag cataatttaa cccataggat cgcaaaatat gttcaagacc
     4261 cggtgtatag ccgaggtttg gtaaccaaaa gccctctgga gcaaggttaa aaaaactctt
     4321 atgagcgatt aaaccagctt ctacttgcgc atggattgct tctttgagat ctatataatg
     4381 tggcaaaaag caatttgttg cagcgcttgc aaaaagttcg atatttcctt tatgtgcata
     4441 ataggcaaat tttgataaga tatcttggtt aaaggtttct acaaaatctc ttttatttcg
     4501 cttacacgtt cgtagatatt cttttgcaag ctcttgggtt tctggatttc cctcgtgttt
     4561 ttttatttca gcctcaccta agagaatgag cttatcaagc cattcgatat aactttgctg
     4621 tacaacaggg tctgttaaaa gggtacaaag tgaaggggtt atggttaaac caagtttaaa
     4681 gttaattcca tcactttcga gatttgcaaa catatttaaa agggggagat aggtttgaga
     4741 aatagcgtaa aagagtagtt ggtttttatc gctctcgcta ttgtttatat cgcgaaaata
     4801 tccttgatgg agtaataaat gtaaggcaaa agactttttt ttcatgtttt tcctctctac
     4861 catagctatt aaataaaggt ttgtcggtga ttattgtaat gttcacgtaa aatatcgtgc
     4921 attcctgaaa gtgcaataat cggacttact tttttatcga taccaggttc gtttttttct
     4981 agaataatat gtggaattgt aattttttgt gtgctagaaa ttattttagg ttcagtattt
     5041 gaaaattcgg ctattaaatg gagtcgaaaa gagttatatg ctggatttag taaaataaat
     5101 tgctcttttt tttctaaatg cacttcaatt tctatgcctt ctttttttac ctcaacacca
     5161 tccttgtaaa aaacaaggga aagagtgaga ccgagatagt ttgggttttg acttatttct
     5221 ttaaattcag aatctcgaat atcccaatag gcaaaacacc agagtggatt gcgcattatt
     5281 gcaccaatat aggtttcatt aaaatttttg ggcagatctt catagaaggt aaatgcactt
     5341 gcatcatcgc gaagatcgtc ttgatgggtt ttgctttgtt cctctgcaat ttccaataat
     5401 tcagaaatga taaatcgtct atttaaagat tcaggaattt caatgttata ttcatctgca
     5461 agcgaaacga gagcactggt tgacattgat tctaaatcag cttttgtaat aatcttattt
     5521 ctcatacaag agctatgatg aacagaataa agcttaatgt caatacctta attctgtagg
     5581 cctttatagg aaaagcctac agaagctgta taaaatcttt attggacgag taaattatat
     5641 gcgtcaagaa ggttttctgt ttctaagaga gcctctcgca agtctttgtt tttgagtttc
     5701 gtactaaaat acgaaagtgt ttgtaaataa tacgcgtgtt gattgtagga ggctgcaatc
     5761 ataaataaga gtcgaacagg tgtatcgtca attgtttgaa aatcttggac atcaacttta
     5821 gaaatgccta cagacataat taggtcagta accgaagaaa gtcgtacatg aggaatagct
     5881 atgccccgtc ctattgcagt tgacataagc tcttctcgtt tgagaatttc gcttactagt
     5941 tcttgtgcgt tttttacttg tggcgcagtg ctgagattgt cagcgagggc aacaagcgca
     6001 tcttgctttg aactgtgatt taagaaaata actctatccg gggagagtat gttttttatt
     6061 tgaatttgtg ctccaaaagg ggaggtgcgt gaacctgttg ataaacggtc gttaacccat
     6121 ttttcaattt ctgttttttt aaatcgccat acagtgccaa ttttaccaga aggaatttct
     6181 cctttttgcg cccagtcgta tactgttctc tctgacactc ttaaatactt tgctacttct
     6241 tcaattgtaa gtatattgtc ttccactcta ttctccttgc atattcagga attgttcttt
     6301 gtttgtattc tttagtatga ttttgcactg tttgtgcgta tatgtcaagg gttgcataga
     6361 aaaaaaatac tatctttgct tgacaagcat atatgcttat tataccatgc ttttatatga
     6421 aaataaaaga aaaaaaatgg tttttttcac ttttaggctt tttaattgtt cttctctatg
     6481 ttttgttttc gagccagccc ttaaaaaaag aactacagct cctgcctgag tggactgtca
     6541 acttagaaga tagctatctt gcccatacaa atgaagataa gctttttgca cagagtgaaa
     6601 cagaagtgct tttacccttt ttacaagggc agtatcttgg ggcagtctat ccaagcggag
     6661 aaattgcctt catagaagca tacgcacaac gagcgagcct ttcaaaaaaa tactggattc
     6721 cgtatgaaat gaatgcaaaa aagactctcg tgcatcctat ggatggagga gagccctttc
     6781 taatcaaaga agaaggcttc ccatttgcta tagattcatt tttgtttacc tttttccccg
     6841 gtggaaatag ctttggctct tatgattccg agggacagct tttgtggagc gcatcgcagt
     6901 ggagtcctat atccgctttt tctgtctctg acaaagctgt tcttgtcggc tacatcgacg
     6961 gaaaccttcg cttgttttct gccgatggcg acttgctttt ttcaagctat cccgcgggga
     7021 gtaagtatga aattatttac ggagcggcac tttcttcaga tagccaatta cttgcctcca
     7081 tctgtggctt agaaaagcag cgttttgttt tatacacagt cgacggcgaa aatacaaaaa
     7141 ttatccatca cgagtatata gacggagaaa aaacaagggc tcgctttatg caatttaatg
     7201 ccgatatgag caaggttttt tatgatgtcg atcagggcct cgctgcattt gatataaaaa
     7261 caaaaacgac aaatcttgtt ccccttactg ggaatatttg tagcatgcaa aatctatcct
     7321 ttgaaaatac aatcgtggta ttaagtaaaa aaaacaatag ctggtatcta tcgcttttag
     7381 atgcctatga cgaaataatc gcaagttttt cttttaccgc agaatctgcc tcgcttttta
     7441 cctacgaaaa tgcattgttt ttgggaaggg atgcgagtct ttcaaaaatg gagcttgtaa
     7501 gaaaatgaaa aaagtagtta aagtacaaaa aatacgcctt tctgttttta tccttagtct
     7561 aatgcttggc actgtctttt tttcacagat gcccatgcag gcttttgaat ggccgcaaga
     7621 aattgcttcc ctaaacgaaa ttttattttt ctttggggag gacaggaagg actttatatc
     7681 ctcttccttg gtttttccaa agggggaggt gataaagctt agtgaagctg gaaaagtact
     7741 gtttcagtat gaagctgagc agtcttgttc tcgctttcct tcgagcttgg gaaacgcctt
     7801 ggtgctcgcc cacgaaaacg atttacgaag tctctatgca aaccttggag aaattgcagt
     7861 ggagaaagat gcggactttt tacaagaagg tttagaaata gcgatgagtg gagattctgg
     7921 ctggcatgag gacggagctg gcttagaatt tgctgttatc gatgtaaaaa ataaggctct
     7981 tgtcaatccc ttgcttgttt ttcccatggg atataaggca atcgattttg cgataggaaa
     8041 aatctacata aaggatgtaa aaggtgatta tagagaactg gggactggaa aaagctttga
     8101 agaaggtttc tatacttttt atcgtgaatc tcgtgataaa atgccccttt ttagaagcct
     8161 tgtatcactc aatggaaaga cgatagatag cctcagctat gattctctgt ctctttggaa
     8221 ggaaaaactt gtccttaaat caagccaagc cgtattgctc gattataaac agctttatcc
     8281 catggaaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ttaaaaaaaa
     8401 gctatccgat tgtggatagc ttttttattt agtcttctcc gtagagatgg tcgaattcat
     8461 gcattttttc cattgcctct tccttgcaac caccacagac ggtaccgatt tttgtgcgcg
     8521 cttgtaagtc agcccagctg cggtcgcctg ccttataggc ttcttctaag tccttgtcgg
     8581 taatgttaag acagctacaa ataacggtta cttcttcctt tccttcaagg cttgctcttc
     8641 cctgtttttc aagatagtcg tttatagcag cacggagcgc cttatctccc aaaacagaac
     8701 agtgcacctt gtttgaaggc agtcctccaa gctttgccac aatgtcttga ggcttaagct
     8761 tataggcttc ctctatgtgc attcctttta ttgcctctga aaggatagaa gttgaggcga
     8821 tagcacttgc gcatccatag gttttccatc gcacatcgct gataacgtca tcttttactc
     8881 gcaaaagaat gagcatttga tcgccacagc gaatatttcc gacaatgccc cgtccatcgc
     8941 agggccattg gtcttcattt tccattaaaa catttttggg gtcagtaaaa tgctctttta
     9001 ctacctcaga atataaccac tcttgcatag ctatttctcc tctatctgtt tatacagtgt
     9061 attcttacat cggaatcgat gacatttttc gaagtctatc gataaccggt ggaaatttct
     9121 ctaaaatata gtctacttct tccattgtgc tatattttcc aaaactaaat cgaatagagc
     9181 catgagcaag ctctggtccc aaacctattg ccataagcac gtgggaaggg tctaaacttc
     9241 cagaagcgca ggctgagccc gttgaaacgg atatacccaa aagatcgagg tggagtaaaa
     9301 tagcctcgcc ctcggctcct ggaaaagaaa cattgagggt gttgggaagc acttcgcttt
     9361 cgtgcccgtt cacgtgaatg ttgggaattt tttctaaaaa gccctctcgt aaggcttctc
     9421 tcaacttcca tgtatgagat ttatagcttg ataattcttt ttttgcaaga cttgctgcta
     9481 aaccaaaggc ggctattgcc ggagcattat aggtccctgc tcgaaagcct ttttcctggt
     9541 gcccacctcg tataagcggt tctatattgc gcccttcttt tacgtacaag gcaccgatgc
     9601 cctttggtcc atagatttta tgcgaggaaa aagttgcata atcaacattc catgctttta
     9661 agtccacagg aattttcccc accgcctgta ccgcatcgct gtgaaaaagc gcaccacttt
     9721 ggtgggctag gtctgcaagg acttgtatat cttgcactgt gcctatttca ttgtttgcag
     9781 ccataatgga aacgaggaga ggattttcac tgagggcttt ttgatactcg tccatattga
     9841 ttcgtccgtc aggattaacc gcaacttcta taagtcgaaa accttctctt tcgagttctt
     9901 ttgcagattc tataacacaa ggatgctcaa ttgcagaaat aacaatcgta tttcgttgca
     9961 ttttttttga tagcgagatc attgtttgaa atacggtatt gtttgattca gaaccacctg
    10021 aggtaaagat aatttcatta gcggagcaat ttaccagctc ggcaaattct acccgtgcct
    10081 tttctatttt tttagattca agtcgcccat cttcgtgtaa gctggacgca ttgccaaatt
    10141 tttccaactc tgagatgtaa aaatgcttta cttcttcaga aagtggtgtt gttgcattgt
    10201 aatctaaata tattctctta tgcattttct atcactcggg aaataatccc gcctcctagg
    10261 ctaagccctt ctttatatat cacaactgat tgcccagggg caacagctct ttgcgcttct
    10321 aaaaactgca ccaaaacact tgtatcttca atgcttggta taatatctgc ttcgactggc
    10381 tttgatgcaa gacgaatttt cacccaggct cgaaagcttt tttcggggcg atagtctcct
    10441 gcccaaaccc agttttctga aagcaaggcc tttgaaaata aatcatcctc ataggcgagt
    10501 attatggtat tgttctgaga atcgatggct tgaacgtaga gcggttttac atcgctaatc
    10561 ccaagccctc ttcgttgtcc gacggtataa tgctcaagtc ccctgtgttt tcccaaaata
    10621 cgtccttcgg gattgataaa ggaaccttct tgtgcttttt tatcttgaaa gaggatatca
    10681 aaatacgttt gtggtacaaa atcttggctt tcttttcgct tcgatgctgc aagttctctt
    10741 ttttttgcca tttcatagac ttcttttttt gtaaaatctg agagggggaa acgtaccttc
    10801 tctaaaatgg atgagggaag gcggcataaa aaatagcttt ggtctttttc gccatctttt
    10861 gcttgagtta caacaaaggg atgtaagcct gtttttgcat cttctccgta gacagaggct
    10921 atatcatctt ttatttgtac aatacgtgca taatggccag tacaaaaata gtcaaagcct
    10981 attccttttg cgtcgagttc tttttggata ccttctataa atgctccaaa ttttacaaag
    11041 cgattgcatt gaatacaagg atttggtgtt ttacccgatc gatactctgc tttaaaataa
    11101 tcgaggacct tttcgttgta gacctttgct acagggatga ggtagtaagg aatatcgtgt
    11161 tctttgcaaa aactgcgaca ttcggctatg tctttttctt catcgggact atagcaagat
    11221 tcacgtctgc ccgattctga gtgcgggata gggtagccct cgtcccataa agacatggtc
    11281 gctccgatta ccgtgcaacc acgttctttg agaagtaagg ctgtcaatgc tgagtcgaca
    11341 ccaccagata aggcaacaac gacagttgag ccagctttgg gagaggatac ttcttgtatt
    11401 gcaataggac tttttttaga aagcccagta ctttcatagg tcatacttaa aatatactgt
    11461 tttaatgaaa aagagtgaat agacaaaggg tataaaaact agcgaaatac taggtttctt
    11521 tagtcaaaat gctttatttt ttattggaac tatagtatat tatgagtatg aagatttcaa
    11581 cacgcggtag atatgcattg aggctcttat tggacctcat gttacaagat aaagatagct
    11641 atgtttctct aaaagaaata tcgagtaggc aagatatttc tgtgaagtat ttggagcaaa
    11701 ttacctcgct tttgacaaag gcgggtttgc ttatcagtac ccgaggccct acgggaggct
    11761 ataagctagc aaaaagccct gatcacatac gagtttatga tgttatggtc gctacagagg
    11821 ggccactgca ttccgtagct tgtttaggtg aaacggaaaa cccttgccct cgaaaagatt
    11881 cttgtactac ccttggagtc tgggaaggat tcgataaggt tgtttacgat tatctaactt
    11941 caattactct ttctgacgcc cttacattgg aagacaaaga agctgttaat ttttctattt
    12001 gacagactct ctcgcagctt catttttccc tctttttatg ctatactatc ttcatgaaac
    12061 aagaaagtaa aagagatttt tttgtttcgg ggaggtaaaa tgaatcgcaa agctatactc
    12121 ggagcagcag tcctgcctca tccgcctttg gctgttggta aggttgccat cgataaaaaa
    12181 gagataatca aaaaaacgat tgcatctttt catagccttg caaaagagat agaaaggctt
    12241 gcaccgcaaa ccattgtcct tatttctccc cacacgaacc tctatgccga ttattttcat
    12301 ctgggatctt ataacgaggg tgaaaaaata gcttggggag acttctcgcg ctttggtgat
    12361 gcagaaagtc gagttgaagt aggcttcgat gagcccttta tcgatgcact ggcagaactt
    12421 tgccaaaaag aagatttccc ctgtggaaaa cttggaaaaa aaacgtcctt tctcgaccat
    12481 ggaattaccg taccgcttta ttttgtgcaa aaacagtata gcgattttca gcttgtgcaa
    12541 gttggcttaa gtggcttagg tttagcagag cactatgcct tgggtatgaa aataacagag
    12601 attgcagaaa agcttgggcg aaacacctat atcatagcaa gcggcgacct ctcccataaa
    12661 ttgcttgccg atggacccta tggctatgct gccgaaggcc cagaatacga tgaacgaata
    12721 atggcggtct tggaaagggc tgcctttggc gaaatgctgg attttgatga agctttttta
    12781 gaaagggcag ccgaatgtgg gcatcgctct ttttgcatta tggcaggagc ctttgacggg
    12841 cttaaagtag atgcaaaaaa actctcctat gaaggtccct tcggcgttgg ctatggtgca
    12901 gctcttttta taccaataga aaaagataca gcacggcatt ttttagaagg ctttagagaa
    12961 aaagaagcta aaaaacttac agaaagacga aaaaacgagg acccctatgt acagcttgca
    13021 cgcgctagta tagaacactt tgtaaactgt ggaaaagttc ttgaactcga tgcaaaatta
    13081 aaagaaaaaa ttgtgctaga aaaagaagcc tatacaaaaa aggctggttg ttttgtttct
    13141 ttgaaaaaaa acggacaact tcgcggctgc attgggacta tttcgccgac acaaaaaaac
    13201 attgccgaag aaattattgc aaatgctatt tcatctgcga gcagggacgg gcgctttgaa
    13261 cctgttcgtg tcgatgagct ttcaagcctc gtgtattctg tagatattct ctcggaatcg
    13321 gaggctattg cgagcgaaaa agagcttgat gtaaaacgct acggagttat tgtaagtgcg
    13381 ggacataagc ggggactttt actgcccaat cttgaaggcg ttgatagcat cgacatgcaa
    13441 gtatctattg ccaagcaaaa ggcgggtatt ttgccacacg aaaaagtgag cctcgagcgt
    13501 tttgaagttc ttcgctatga ataaaaaaca ccttgtaaac tgcgatattt gtcatcatcg
    13561 ttgtaagata gctctgggag cctatggttt ttgtggtatt cgccaaaacc tcgatggaaa
    13621 acttgtaaac caaggctatg gaaaaataac agcccttgcc ctcgacccaa ttgaaaaaaa
    13681 accgctttat cgtttttatc ccggttctgc tattctttcc gttggttctc tcggttgcaa
    13741 tatgaactgt agtttttgcc aaaatcacca cattgctcgt ttacgcctcg attctgccga
    13801 gtcaaattct gcttgggagt cttatacgcc aaaagccctc gtcgaacttg caattgaaac
    13861 caaaaaacaa ggcaataggg ggcttgcctt tacctataac gagcccctta tcaattatga
    13921 atttattatc gattgtgcaa aaaaaatgaa ggaaatggat tcggagctta aagttgttct
    13981 tgtaacaaac gggcatctag aaatagctca tgcaaaaaaa ctccttccct acgtcgatgc
    14041 ggcaaatatt gaccttaaag cctttcggaa agccttttat acaaagatgg gtggtgattt
    14101 ttcccttgta aaagccttta tcgaagaagc ggtaaaaaac tgccatgttg aactatgcta
    14161 tttgcttata ccgggcttaa atgatggact tgatgaagtg gaagaagccg ccgagtggat
    14221 tgcttcccta gattgcaccg ggcaaggacc cctggaaaat ggaaaagaca tagccttgca
    14281 tatttctcga ttttttccga cctataagct cgtagatagg gctgcaactt cagtttccgc
    14341 tgtctatgag gcagtcgcag tagtaaaaaa acacttaaac tgggtttatc caggaaatgt
    14401 ttgagcttta atctcggttt ttatttggct atcatagaaa aaaaacgagc aactcttaac
    14461 atattgacaa agtttgtcat tttggcaaat tttttatgtt ttttgctgtc tacgtattga
    14521 ttgctcttgt aacaaagtgc agacttaatt atacttatcg aatagtaaaa gggaggtttt
    14581 tttgtgccaa aaggtaaacc aattatcgat atagaaaaat gtaagggctg tgccctttgt
    14641 gtaacagctt gtcccaaaaa aattatcaat atgtcgagtc aaacaaatgc gcaaggggta
    14701 cattttgcct attgtgtaga tgaaacgaag tgcattgcct gtatgtcttg tgcaacgatt
    14761 tgcccagata tggtaattga aattcttaaa tttgattagc taaggataga aatatgaatg
    14821 ataaaatatt aatgaaggga aatgaggcaa taggtgaggc tgcaattctt gcgggctgcc
    14881 gccattattt tgcctatccc ataacaccac aaaatgaaat tcctgcctat atggcgctgc
    14941 ggcttccaga agttgagggc acatttttac aagcagaaag tgaagttgcc gcaatcaata
    15001 tggttttcgg ggcttccgct gctggtgcgc gcgttatgac ctcctcctca tctcctggaa
    15061 ttgccttaaa acaagaaggt ttttcctatc tcgctggtgc ggaacttcct gccgttgtgg
    15121 taaatatgat gcgaggtgga ccaggcttgg gaaatatttc tggctcacag ggtgattatc
    15181 atcaagcaac tcgtggtgga ggaaacggcg actataaaac catcgtttta gctcccggaa
    15241 ctgtgcaaga acttgcagac cttacgataa aggcctttga acttgccgac aaataccgca
    15301 tggtcgcaat gattttaggc gatggctacc tcggacaaat gtctgagcct ttgactcttc
    15361 caaaagcggt tcaaaacatt ccgtcaaagg cagactgggc gctcactggt cctaagggtc
    15421 gagctggtcg ctcaattgca tccctcagac ttgctccaga tgacgcactc tatcaacaca
    15481 atatgcactt agcagaaaaa tatgcccaag tcgaggcagc agaaacaatg gctgaatcct
    15541 atctttgcga tgatgccgat attgtacttt gtgcctatgg tacttctgct cgtatttgcc
    15601 gaaaagcaat aaaaaaactg cgaagcgaag gcataaaggt tggacttttt cgtcctatta
    15661 gtctctggcc ctttcccaaa aaagagcttg caaaggttgc atcaggggca aaaaaactgc
    15721 ttaccgttga aatgagcctt gggcaaatgc tcgacgatat tcgtttacat tcgggtaaaa
    15781 tcccctctga ggtcgatttt tacggtacac ctggtggtgc acttccaaaa attgaaacca
    15841 taatagaaaa ggtgaggtcc tatgtctaag ctaatatata caaaaccaga agcccttcgg
    15901 ccggtaaaaa cacattattg tccaggttgt ggacatggca ttgtgcataa actcattgca
    15961 caaattatcg atgagttggg tattcgagaa gatacccttc ttgttgcacc cgttggctgt
    16021 gcagttcttg cctataacta catcgatgta gacggcttgg agtcagccca cggaagagct
    16081 cctgcaatat ccacgggact taaaagagta cacaaagata aaatcgtgat ttgctatcag
    16141 ggtgatggag acttacttgc cataggggca gctgaaacta tccatgcggc aaataggggt
    16201 gaagatatta cggttatttt tataaataat gccatctatg gtatgacagg tgggcaaatg
    16261 gcgccgacaa gccttgaagg tcaggttacc aagacaacac cgaagggacg agatgcaagc
    16321 gaggttggct acccaattcg cgcttgtgaa atgctcaata cccttgttgc acccgtttat
    16381 atcgaacgat gcgccgttta tgacgtaccc catatcaata aaacaaaggc agcgatacgc
    16441 aaggctcttc gctaccaaaa agaaggcaag ggttactcct ttgtagaaat tctttccatg
    16501 tgtcctacaa actggggcgt cgatcccgtt aaaggcgctg aatgggtaaa aaatgcaatg
    16561 gaaagctatt atccactggc tgtgtttcgc gacattcctg caggaaaggt tccagaacta
    16621 gcagcaccag agggaacatc agcctatgag cgaagggagg aagcacgatg acaaaggaaa
    16681 tcatttttgc aggctttggt ggacaaggcg tcatcctcgc gggaaaggta ctctgccttg
    16741 cctgtatgag cgatggaaaa tcagtctccc atattcccgc ttacggggct gagatgcgag
    16801 gcggcaccgc gaattgttcg gttattattt ctgatgaaga catagcctct ccagttatcg
    16861 ataaaagcga catcgttatt gccttgaata aaccctcaat ggtgaaattt gaaaaaaacc
    16921 taaaagaaaa cggcttactc atttataata catcccttat cgatttaaaa ccaagccgaa
    16981 cagatatcaa aacacttggt atccctgtga atgctatggc agaagaaagc ggtaacccac
    17041 gtggaatcaa tatggttgtc ctcggcgttt tggcaaaact ttttcccgaa atgctttcca
    17101 agcatgcgca ttttactgcc gctctcgatg aagcaatctc agagcgaaat aaaaagttta
    17161 atcccttgaa tattgcaacg attgaaagcg catatgcaaa agactatgtt ttagaataag
    17221 gaagaaaaat atggaaaata aaaacagcct tggaaaaaaa gtacagttat ttagagagat
    17281 gcgaaacctc tcccttgaag aagtgtcaaa acgaagcgga ctgtgtattg atgaaataga
    17341 aaaaatagaa aagggagacc tcataccatc tcttacaccg cttattaaaa tagctcgtgt
    17401 tttaggaaca cgactcggct catttcttga cgatgatgaa aatctcggtc ccgttgttac
    17461 acgaaaagaa acacatacat caattacccg tttttcaaac agagacggcg cggtaagctc
    17521 agacttggat tttttctccc ttgcacaaaa caaaacatcc cgacatatgg aacccttttt
    17581 aatagacatt catccattgt cagaaaaaga atacagaatg tcatcacacg aaggcgaaga
    17641 atgtatctac gtgctctctg gttcgattga agtaaaatat ggcagcgaaa cccatgtgct
    17701 aaacgaaggc gatagcatct attatgattc aatagtagaa catcatgtgc actcagcatc
    17761 tggagaagca gc
//
LOCUS       DAOSSW010000030        17658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_3334_length_17658_cov_8.072488, whole genome shotgun sequence.
ACCESSION   DAOSSW010000030 DAOSSW010000000
VERSION     DAOSSW010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 17658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17658
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3334_length_17658_cov_8.072488"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1802)
                     /locus_tag="PKW26_04075"
     CDS             complement(66..1802)
                     /locus_tag="PKW26_04075"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ92777.1"
                     /translation="MKLKAELLVFIFVSIFILFSCTTTKPSTSFIESDEVQKKPREAV
                     AITTPRISKKKFSSIEPSLLQKIENARAGDLQEAIAVLKKKEPKLSKDEESLLRIAYA
                     LQAILYQEKIDESLYTDSTDLDSYAATIAAAKRGIYDFNSGTEDFFSLSLPSLVLITA
                     PSVHNYYEEARFALEKALSLNPESFFAEYLFAILLEEGFSLDEAIPRYKNAHEKNPSY
                     IPTMLRYMKALAKIGQEKEAFQKAELILAQEDHNIDALWIAARYSFSEKKYDAAEMYI
                     SRLLQRDPNNSEFILFRAKVLMAKEEFMKASSLLDLYAKTDKTAREYLELKALLQRDW
                     NKNPTAAASTLQEALRLYPDDLELLLIAASISAQLGQKVADFSLEELLDRILVKEPQN
                     SEAIAIRVDEFILQEQWQDAYAANSSLYAQAKKDPHVLLNEIKICLALNKVSEAREAY
                     DFLQVLTGENEETMLSNIRILIAEKAETSARSLIETNLGKATNTRTKSALYFERSKLA
                     SSDEARLNDLRFSLTANPRNADALFSLYNYYFQQKEYRKAQYYLKQAVSLNTNSKILL
                     ERNKELDILLAE"
     gene            complement(1799..2371)
                     /locus_tag="PKW26_04080"
     CDS             complement(1799..2371)
                     /locus_tag="PKW26_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92778.1"
                     /translation="MKKKKDIQIANIMVSPMNKMILFFGLILVCFGILLITCMRPLCS
                     TAFFAFRSFACVSLGAIFFYLSLIHIKKTAFFFLGLLFQLIGFLFILIDLGLISYAFS
                     QLWPVVLMFAGLCLFVTNFYKQSRILKQFLVPAILLFCLGFVFLLFSLHLFNFTFSQF
                     VVDWWPVFIVVMGIILVVLFFINKKKVPVE"
     gene            2436..3470
                     /locus_tag="PKW26_04085"
     CDS             2436..3470
                     /locus_tag="PKW26_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ92779.1"
                     /translation="MNITNKIACFLLSVYVFTFFSCTEPNFLDKHQKDLFSILKEPNL
                     SNETTFSVINQIASTYKTQKKESELILFLTDYVEKNPSDPYNAYWLLMIALFYMESDA
                     EKVAEYYFDRIIQNYDDLLVKDKSLHIISLQNLIKMSDSPENKVSYYNALISQFGEEL
                     NITELYLRLAQEYEAMGDWELALKSYASFLERPDATTIQIPGISDPYNTAKKLVDFNN
                     SPKNQTFETLEALETAIKRAIDTRNFNILDSYCANVNFFAMSWKQDDNADNVEKNFSM
                     RDYGLGNRIYYDKNLDESSNPNEAYLRTTGWSRYISTWYLYFRKVNFPLDPEIHGRWE
                     WAGIYFGEKV"
     gene            3474..4709
                     /locus_tag="PKW26_04090"
     CDS             3474..4709
                     /locus_tag="PKW26_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOQ92780.1"
                     /translation="MKRFFQYSAILLLLFSQILFASESLELKAIDIDVPEEELISYYK
                     NQYLSDSAKRWLEEIMTNAAEYRPFILQELKEQDAPLCLQYLPVIESNYKITALSKSG
                     ASGLWQFMKNSIAPFNIHVNEWMDERRDPWLSTRAAIQKLKENYSYFQDWHLALAAYN
                     AGLGGISRLMQSYKTNNYWDLAKKGALSTETKHYVPKFLAIAEILENHEYYGLNFPSP
                     EETEHKSLDFVEIPIKKAVSLQILAEKIEVSEKELIYYNPSLYYGISPMDTSYRLRVS
                     QDSAEKTLALLNDESLPLIQYSMYKIKSGDTLYALALHYGVSVDSIQKLNSLKTSKII
                     IGQNLLIPSIKNVGEYAGSKPAEAIAYDGSYVVKKSDTLWSISLAYNVQVEQLAEENN
                     MLVSDILREGFILKVPIRN"
     gene            4719..6077
                     /locus_tag="PKW26_04095"
     CDS             4719..6077
                     /locus_tag="PKW26_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOQ92781.1"
                     /translation="MEASTGSKKRIIFATALIFFICLRSIPLFSQAYSSQSAVNWATK
                     KFNSRLELDFSQAGLKHPVDRGSAVDALSSRLPSLIKEPLLSIIVDSSRSLGDLILLD
                     ELRFEVLTEITKTAQKSAGVFINNGQDMEMYHTIDLENIASLLIKHKTTYTQQIPIQS
                     LSSKPYTGILIDARGSLPVQGEFLNEKLHPSLFPKIWTDAMELIYEKNMVEPSIALRK
                     GLVSYADRINEKEFAARIGNDPLRIKAQKVFGVYRTDPVISRDDALKILSVPQNLELL
                     KNGKVIILVDSDLLSHAVVAPEKTATYYAVFKEFLELAYDKRFPDKIEDTHKGIQITS
                     HFNFIADSPELLANEKEHLDSIARLLQQHALSNEYTIEIEGHTASVGKPEGEMILSIQ
                     RAETILSELQNRGIDTSSFSYRGYGGTLPIGDNETEEGRAENRRVEIYVVPKATYIQR
                     SN"
     gene            complement(6157..6813)
                     /gene="purN"
                     /locus_tag="PKW26_04100"
     CDS             complement(6157..6813)
                     /gene="purN"
                     /locus_tag="PKW26_04100"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOQ92782.1"
                     /translation="MKREKMQNKALRLAVLVSGGGTNLQAIIDSERAREQSPFRVSLV
                     ISSTKDAYALERARRFAIQSEITSPVSVLGSEKAKTATMEEKRLALSNAVFELCKKND
                     IDLIVLAGFLTVLTGPIIKAYENRIINMHPALLPKFGGVGMWGNHVHEAVLASGEMES
                     GCTVHLVDSGCDTGEILVQKKVPVLQDDTAETLYKRIAPLEHEAMIEALEMLSARLLG
                     "
     gene            complement(6827..7876)
                     /gene="purM"
                     /locus_tag="PKW26_04105"
     CDS             complement(6827..7876)
                     /gene="purM"
                     /locus_tag="PKW26_04105"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOQ92783.1"
                     /translation="MVDYKDAGVDIEEGYKAVSKYKDFAKATMIPGVLNGLGSFAGMF
                     AVPKGIKNPVMVSGTDGVGTKLDIAFAMKKYDTVGIDCVAMSVNDILCTGAKPLYFLD
                     YIACGKLDSEIAADLVKGISEGCLQTGCALLGGETAEMPGFYDEGKYDIAGFGVGIID
                     KKKIVTGDKIQAGDVLIGLASSGVHSNGFSLVRKLVSDFDTAFEGTTIGEVLLEPTKI
                     YVKPVLAVLEKYKKAVHGMVHVTGGGFYENIPRMFPKNNEGQFLHRVVIKKKSWTILP
                     IFDELVKRGANSDRMYHTFNMGIGFVLAVDAKKAASIIETFKQHGEEARIIGRVEALE
                     KPAFLSQEEALVFED"
     gene            complement(7935..9473)
                     /gene="purF"
                     /locus_tag="PKW26_04110"
     CDS             complement(7935..9473)
                     /gene="purF"
                     /locus_tag="PKW26_04110"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOQ92784.1"
                     /translation="MNINQGDFKKNMLSHIIDENAEDKLKDKCGVIGIFLNTEGCSEN
                     TEIAADSKTKTKIQSKELLGQNAAKLAYYGLHALQHRGQESAGIAVSNNGEVEQIRGL
                     GLVAEVFKENKLQGLSGNIACGHVLYETTNNVHIENVQPFVNRSKLGTIAVSHNGSLV
                     NYEQIKEFLEETGSTFVSTSDSEVIIKLIAKSYKKGLDRALTDTIQMIKGSFSLVVMT
                     ENSLIGARDTHGIRPLCLGQLENGWVLASESSALDAIGAELVRDIRPGEIIIINKDGV
                     LSFDFGEQTAKHTCVFEYIYFARPDSIMDGIAVQEARLRMGAVLAKESHVDADVVVGV
                     PDSGLGAAMGYANASGIPYAMGIVKNKYIGRSFIAPAQSSRENIVFVKLNVLKSDVEG
                     KRVVVIDDSIVRGTTSRRLIEILRRAGAKEVHFRISSPPVKFPCYFGIDTPSRQDLIS
                     SNFSLEKTREQIGADSLAFISIEGMLEALKGIDEKNYGYCKGCFTGKYPIAVPMAGLN
                     TNGN"
     gene            complement(9516..10013)
                     /gene="purE"
                     /locus_tag="PKW26_04115"
     CDS             complement(9516..10013)
                     /gene="purE"
                     /locus_tag="PKW26_04115"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOQ92785.1"
                     /translation="MKVALFFGSKSDTEIMKKAGDVLKAFDVSYKAFVLSAHRSGDLL
                     KERIREVEAEACQVIIAGAGLAAHLPGVIASHTILPVIGIPLECRSPQSNGLAGMDAL
                     FSIVQMPKQIPVATVGINNAENAAYLALQILALSDADLKEKLILFRKKLVDEALLNGA
                     CGVDL"
     gene            10176..>11926
                     /locus_tag="PKW26_04120"
     CDS             10176..>11926
                     /locus_tag="PKW26_04120"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOQ92786.1"
                     /translation="MILDNENENLKVHEWIQEYTEQGSLDIITGYFTIGALAWLSKTV
                     NDKITDFKLILGDIVNIDSVENRPLDLLNENISIEASLKLSSLAKEAVNFLKQDKVVA
                     KTLEPNFCHAKTYLFNPNKNDDRNKYFISGSSNLTEAGIGLKQTNNVELNIAETGNNN
                     QYKELSTWFHEIWNKPQAHKKKTLIDSEGKKTSVDFKNYLIGEIEQIFIKYSPRELYY
                     KTLFELFGNQLLETESDPNFNRQIGRLENSAIYNTLYEFQKKGVLSLIRMLQKYNGAI
                     LADAVGLGKTWSALAVIKFFQLQGRETMLLCPKKLEHNWQRYLRNQDSRFEKDQFDFF
                     IRFHTDMHEDRMEKYTDRNDKYFTNDKPKLIVIDESHNLRNDKSNRYKFFLEEILKKN
                     EDIKVLLLSATPINNSLTDIRNQFKLMVQGRVDGYEDSLGIRNLDYTFRVAQNTFNEW
                     RKDDKPRIAEFIKKLPENFFTLTDSLIVARTRKMIEGQQTGLTFPLKAKPENHFVTPS
                     QLGNFESFEELFDHFPPMLSGYQPSFYLDEDETDILHDEKQRDRFLVKMMYILMVKRL
                     ESSWYSFYSTIEKIKDH"
     assembly_gap    11927..12026
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12027..13422
                     /locus_tag="PKW26_04125"
     CDS             <12027..13422
                     /locus_tag="PKW26_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOQ92787.1"
                     /translation="NLQKFKEKIESETVLPRNQESADDKLQTLIKEINEKRKLGENNG
                     NKKVVIFTVYRDTALYLYNQLKSRGFDKLAMVSGTGSISSDSKEETKNFESILERFAP
                     YTKLYMEKEWDFKSEKKGIEAYHEWISWIAQNHKKTYEKVQNPIDILIATDALSEGQN
                     LQDADMVINYDIHWNPVRVIQRMGRIDRLGSPNKKIFGINFWPSNNINSYLNLQGRVE
                     ERMATMKLAGSEVDEKFSKSFKEMSHDESFDQKMNNRMLEQMKTTFDDLEGEKAFGFD
                     DLSLEGYRQDLVEEFNRDKAKYQKMPKGVYTGFKADSAICAENGLIAVLGYPAKAPKS
                     PQHEYTKYNLIYINSEGKSVLLNQKEILDALTLHKEKDRFVPEKIDSGDEEAIQDLAG
                     SLKSWLESQAVEEVAQEDGTTKKQMGIEAKNILAKLRKGDKDAVSKVKQNIKVEEKFQ
                     MENFDLITWFLVTV"
     gene            13434..14570
                     /locus_tag="PKW26_04130"
     CDS             13434..14570
                     /locus_tag="PKW26_04130"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOQ92788.1"
                     /translation="MKFDDLIVQIQEVSKNLQQQAVKAVNVSLTLRNWLIGFYIVEFE
                     QNGEDRAQYGDRLLERIAETIQIKGLGITNLKWCRIFYQNYPQIIQPITQMFDNPIFL
                     PIRQSITDELQSTKNQMHKFHPLPIDECNKSQKHIQHLLNHCSFTHFIELIKIDDAIK
                     RKFYEMLILKTTPSIKELKRSIESLTYERFGLSENKEKALQKIINKIQPQSNTDLIKS
                     HYFFEFLNISHPELIEESDLEQALITHLQQFITELGNGFCFENRQKRILIGDEYYFID
                     LVFYHRILKCHVLIELKTEAVKHEHIGQLKTYLNYYKKNIQEPNDNPPVAILLITEQN
                     KALVEYAIADADKDLFVSKYQLQLPRKEEIEKFITNELKRLKNE"
     gene            14563..>17658
                     /locus_tag="PKW26_04135"
     CDS             14563..>17658
                     /locus_tag="PKW26_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOQ92789.1"
                     /translation="MSKLEKFKTDSFIEATQSFFKDLGLPILNITEEPTSAKETLKDT
                     YKENESFQLIDDVYFVGMVDDAAFENKTPLNPSEIKNEIERDYDGILIFALTLNKRPN
                     DLLPTRSQLAEISRAFNREFYYTPVVIVFKYNNYLAFANTERLKYKQEWREGEKAGKV
                     SLLRDIYIEKPHSGHERILSELEIPSSGTKKINTFATLYAHWQEVFSVNLLNKKFYQE
                     LSNWYFWAIKNVVFPSEPTIEDAHRKNAKLEDLIHEHRATNVIRMLTRLLFVWFIKEK
                     KLIPEELFELESLQKDILNEIEPFYENGLFTESNKDSVYYKAILQNLFFATLNCPILS
                     DDLDKRFRGFRREKNYGDDFGNDWLYRYKKYFKNPDIFLELMNSTVPFLNGGLFECLD
                     DKPQKIYIDGFSDNLAKGEQLIVPDYLFFGLEEEVDLSDVVGISDAKTKQSAVKGLIN
                     ILKSYKFTITENTPIEEDVALDPELLGKVFENLLASYNPETKTTARKQTGSFYTPREI
                     VNYMVDESLIAYLENAIKDWEGLNEKEIDEKLHQLTSFDTAEVFADKPDLQKKIVMAL
                     STCTILDPACGSGAFPMGILQKMVHILQKLDSDNKIWYDVQLEKAEQESKDAFKIDNK
                     EDRGERLQEINEVFDRSINDPDYARKLFLIENCIYGVDIQPIATQISKLRFFISLITD
                     QKVSPEKTNFGIRPLPNLETKFVAANTLIGIEKESGLFFTEDIQELEKKLKVIRSKLF
                     NARTKETKNKYRKKDEELRTEIAKILKNNGLPLDSAEKLATWDPYDQNASSPFFDPEW
                     MFDIEDGFDVVIGNPPYVQLQKNTGALAKEFENANFETFTRTGDIYSLFYEKGINVLK
                     QNGHLTYITSNKWMRAGYGKSTRQYFTKFNPIKLIDLGSGVFESATVDTNILIIQNAK
                     NKNKCLAVDLSKTKENVDLNNLNYEILKNLSSESWTIANKIEQSIKAKIERIGTPLKE
                     WDIQIYRGVLTGYNEAFIIDGETKDKLIAQDPKSAEIIKPVLRGKDIKRYKAEFADKW
                     LI"
BASE COUNT         6011 a         3464 c         3185 g         4898 t
ORIGIN      
        1 ctttaaactc gccatggatg gcgagaggca acaaataatg taagaacact atacagtagc
       61 ttttcttatt ctgcaagtaa aatatcgagt tctttattgc gttctaaaag tattttacta
      121 tttgtattga gcgagactgc ttgtttcaaa taatactgtg ctttgcggta ttctttttgt
      181 tgaaaataat aattatacaa ggaaaaaagg gcatcggcat ttcgcggatt tgctgttaaa
      241 ctaaaacgca aatcattaag cctcgcctca tcagaagatg ctaacttact tctctcaaaa
      301 tataaggcag acttagttct cgtatttgtt gctttcccaa gatttgtttc tattaaagag
      361 cgggcgcttg tctcagcttt ttcagcaatt aaaatacgga tattagaaag catggtctct
      421 tcattttcac ccgtaagcac ttgtaaaaaa tcataggctt ctctcgcttc agaaacctta
      481 ttgagggcaa ggcagatttt gatctcattc aataaaacat gtggatcttt ttttgcttgt
      541 gcatagagac tgctatttgc ggcataggca tcttgccact gctcttgcaa gataaattcg
      601 tcgacacgaa tcgctatcgc ttcagaattt tgtggctctt ttacgagaat gcggtcgaga
      661 agttcttcta agctaaaatc agcaactttt tgtcccaatt gtgcggaaat tgaggcagca
      721 atgagcaaga gttctaagtc atctggatag agacgaagcg cttcttgcaa agtacttgct
      781 gcagccgtcg ggtttttatt ccaatctctt tgtaaaagcg ctttcaattc aaggtattct
      841 ctcgctgtct tatcggtttt tgcatacaag tccagtaagg aagaagcttt cataaattct
      901 tcctttgcca tgagcacttt tgcacgaaaa agaataaatt cgctattgtt tgggtctcgt
      961 tgcaacaatc tcgatatata catttcagca gcatcatatt ttttttctga aaacgaatag
     1021 cgagcagcta tccaaagcgc atcgatatta tgatcctctt gagctaaaat aagctcggct
     1081 ttttgaaagg cttctttttc ttgccctatt ttagcgaggg ccttcatata gcgaagcatt
     1141 gtaggaatat aggatgggtt tttttcatga gcgtttttat aacggggtat agcttcatca
     1201 agagaaaaac cttcttctaa aagaatggca aagaggtatt ctgcaaaaaa actttctgga
     1261 ttaagactca gtgccttttc taaagcaaaa cgagcttcct cgtagtaatt atgtactgaa
     1321 ggggcggtta ttaagacaag cgatgggaga gaaagggaaa aaaaatcttc agttcctgaa
     1381 ttaaaatcat aaatccctct tttcgcagct gcaattgtag cggcataaga atcaaggtcc
     1441 gtactatcag tatataaaga ttcatcgatt ttttcttgat ataaaattgc ttgcaaggca
     1501 taggcaattc gtagcaagct ttcttcatcc ttagaaagtt ttggctcctt ttttttcaaa
     1561 actgctatcg cttcttgcaa atcccctgcc cttgcgtttt ctattttttg cagtaaactc
     1621 ggttcaatac ttgaaaactt cttttttgag atacgcggcg ttgtaatcgc aacagcttcc
     1681 cttggttttt tttgcacttc atcgctttct ataaagctcg ttgacggttt tgttgtcgta
     1741 caagagaaaa gtataaaaat ggacacaaaa ataaaaacaa gaagttccgc ctttagcttc
     1801 attccacagg aaccttcttt ttattgataa aaaataaaac aaccaaaatg atacccataa
     1861 caacaataaa taccggccac caatccacta caaactgtga aaatgtaaaa ttgaaaagat
     1921 gcaaggaaaa caataaaaaa acaaaaccga ggcaaaacaa aagaattgca ggaacgagaa
     1981 attgttttaa aatacggctt tgtttataaa aatttgtcac aaaaaggcaa agcccagcaa
     2041 acatgagaac aactggccat aactgagaaa aagcatagga aataagccct aagtcaatca
     2101 aaataaacaa aaaaccaatc aattgaaaaa gtaagcccaa aaagaaaaag gctgtttttt
     2161 ttatgtgaat tagtgataga tagaaaaaaa tagcacccaa gcttacacat gcaaaagagc
     2221 gaaacgcaaa aaaagcagtg gaacaaagag gtcgcataca tgtaataagc aagattccaa
     2281 aacaaactaa tatgagacca aagaaaagta tcattttatt catggggctg accataatat
     2341 ttgctatttg aatgtctttt tttttcttca tcgcagagtc ctttataaat agtatcggtt
     2401 gcaaattata acaagtctat gataccatac tatctatgaa tataacaaat aaaatagcat
     2461 gttttcttct ctctgtatat gtttttactt ttttttcttg cacagaaccc aactttttag
     2521 ataaacacca aaaagactta ttttccatac tcaaggagcc aaatctttcc aatgaaacga
     2581 cattttctgt cataaatcaa atcgcttcta catataaaac acaaaaaaaa gaaagcgaac
     2641 tcattctctt tttaacagat tatgttgaga aaaacccttc agacccatac aatgcctact
     2701 ggcttctcat gattgccctc ttctatatgg aaagtgatgc cgaaaaagtt gcggagtatt
     2761 attttgaccg catcattcaa aattacgatg acctactcgt aaaagacaaa tcgctccata
     2821 ttataagctt acaaaatctc ataaaaatga gcgattcccc tgaaaataag gtgagctatt
     2881 acaatgccct catttcacaa tttggcgagg agctaaatat cacagaactt tatttacgcc
     2941 tcgcgcaaga atacgaggct atgggtgatt gggaattagc attaaaatcc tacgcatctt
     3001 ttttagaacg ccccgatgca acaacgatac aaatccccgg tatatcggac ccttacaaca
     3061 ccgcaaaaaa acttgtggac ttcaataatt cacccaaaaa ccaaaccttt gaaaccctcg
     3121 aagcccttga aaccgcaata aagcgtgcaa tcgatacaag aaacttcaat attctcgata
     3181 gttactgcgc aaatgtcaac ttttttgcta tgtcatggaa acaagatgac aacgcggaca
     3241 atgtcgaaaa aaacttctcc atgcgcgatt acggcttagg gaatcgtatc tattatgata
     3301 aaaatcttga tgaatcttct aacccaaatg aggcctattt acgaaccacg ggttggagtc
     3361 gctatatttc tacctggtat ctttatttta gaaaggtaaa ttttccgctt gatcccgaaa
     3421 tccatggacg ttgggaatgg gctggtattt actttggaga aaaagtttag gctatgaaaa
     3481 gattttttca gtattctgct atcctccttc tattattctc acagatactc tttgcttctg
     3541 aatccttaga actcaaagcc atagacatag atgtccccga agaagaactg attagttatt
     3601 ataaaaacca atacctcagc gattctgcaa aaagatggct tgaagaaatt atgacaaatg
     3661 cggcagaata tcgtcccttt attttgcaag aactcaagga acaagatgcc ccgctttgtc
     3721 tccagtattt gcccgttata gagtcaaact ataaaatcac tgccttgtca aaatcggggg
     3781 caagtggcct ttggcaattt atgaaaaaca gcatcgcccc ttttaatatt cacgtgaatg
     3841 agtggatgga tgaaaggcgc gacccctggc tttccacccg agcagcgata caaaaactca
     3901 aagaaaatta tagctacttt caggactggc acctcgccct cgctgcgtac aatgccggac
     3961 tcggcggtat ctcccgcctt atgcaaagct ataagacaaa caattattgg gaccttgcca
     4021 aaaaaggagc tttgtcaacg gagacaaaac actatgtacc caaattttta gctattgctg
     4081 aaattttaga aaaccatgag tactacggtc tcaactttcc aagtcctgag gaaacagagc
     4141 ataagtccct cgactttgtg gaaatcccca taaaaaaagc tgtctccctt cagattctcg
     4201 cagaaaaaat agaagtgagt gaaaaagagc tcatctacta caatccctcg ctctactacg
     4261 gcattagccc catggataca agctatcgtc tacgagtttc gcaagatagt gctgaaaaaa
     4321 cactcgccct tctaaatgat gagtccctac cgctcattca atacagcatg tataaaataa
     4381 agtcagggga tacgctctac gccctcgccc ttcactatgg cgtgagtgta gatagcatac
     4441 aaaaactcaa ctctcttaag acatcgaaaa ttatcatagg gcaaaattta ctcataccaa
     4501 gcataaaaaa tgtcggcgaa tatgcagggt ctaaaccagc tgaggcaatt gcctacgacg
     4561 gaagctatgt cgtaaaaaaa agcgataccc tctggagtat ctccctcgcc tacaatgtcc
     4621 aagttgaaca acttgcagaa gaaaataata tgcttgtttc cgacatttta cgcgaaggat
     4681 ttatcctaaa agtgccgata cgtaactagg ggtatagtat ggaagcgtca acagggtcga
     4741 aaaaacgaat aattttcgcc acagctctta ttttttttat ttgtctcaga agcattccgc
     4801 ttttttctca agcctattct tcccaaagcg cggtaaactg ggcaactaaa aaatttaatt
     4861 ctcgccttga acttgacttt tctcaagcag gactcaagca tcctgtagac agaggaagcg
     4921 ccgtagatgc actgtcctca cgacttccct ctcttataaa agagccactt ttaagtataa
     4981 tagtagactc ttctcgaagt ttaggcgacc ttatcttact cgatgaatta cgtttcgaag
     5041 ttctcacaga aattacaaaa actgcgcaaa aatcagccgg tgtttttatc aataacgggc
     5101 aagatatgga aatgtaccat actattgact tagaaaatat cgcatccctt ctcattaaac
     5161 ataaaacaac ttatacgcaa caaataccca ttcaaagcct ttcttctaaa ccctatacag
     5221 gcatcctcat cgatgcgcga ggtagcttgc ctgtgcaggg agaatttttg aatgaaaaac
     5281 tgcatccaag tttatttccc aaaatttgga cagatgctat ggaattgatc tatgaaaaaa
     5341 acatggttga gccaagcatt gcacttcgaa agggcttagt ctcctatgcc gaccgcataa
     5401 acgaaaaaga atttgctgcc cgcataggaa atgatccatt gcgcataaag gcacaaaaag
     5461 ttttcggtgt ctatcgaacc gacccagtca tttctcgaga cgatgcactt aaaattctct
     5521 ctgttccaca aaacctagaa ctactcaaaa atggaaaagt tattatcctc gtcgattctg
     5581 atttgctctc tcatgctgtc gtggcgcctg aaaaaaccgc tacctactac gctgttttca
     5641 aagagttttt agaattggcc tatgacaaac gctttccaga caaaatagaa gatacccaca
     5701 agggcattca aataacctcg cactttaatt ttattgcaga ttctcctgag ctactcgcaa
     5761 atgaaaaaga acaccttgat tcaatagctc gcctactgca acagcatgcg ctttcaaatg
     5821 agtacacgat agaaatagaa gggcatacgg caagtgtcgg aaaacctgaa ggggaaatga
     5881 ttttatctat tcaaagggct gaaactattc tatcagaatt acaaaaccgt ggtatcgata
     5941 caagttcctt tagttaccgt ggatacggcg gaacacttcc cattggcgat aatgaaactg
     6001 aagaaggtcg ggcggaaaat cgtcgcgtag aaatctacgt ggtgccaaag gcgacctata
     6061 ttcagcggtc aaattagcag ttagatgtga aaaacaataa gacaagatag ctgccaaagc
     6121 aagctatatt caagggtcaa attaagtcag gctaaatcag cctaaaagtc gggcggaaag
     6181 catttctagg gcttcaatca tagcctcatg ttcaagggga gcgatgcgtt tatacagggt
     6241 ctccgctgtg tcatcttgta aaacaggcac ttttttttgc acgagaattt ccccggtatc
     6301 gcagcccgaa tcgacaaggt ggacggtaca gcccgattcc atttctccac ttgcaagcac
     6361 agcttcgtga acatgattcc cccacatgcc gacgccacca aattttggca ataaggcggg
     6421 atgcatattt atgatgcggt tttcataggc ttttataatg ggaccggtga gcacagtgag
     6481 aaaacctgca agcacaatga ggtctatgtc gttttttttg caaagttcaa aaacagcatt
     6541 tgaaagagcg aggcgttttt cttccatggt tgccgttttg gctttttcgc tgccaagaac
     6601 agaaacaggg ctggtgattt cgctttgtat agcaaaacgc cgagcgcgct cgagggcata
     6661 ggcatccttt gtgctactga tgacaaggga aactctaaag ggagactgct ctctcgccct
     6721 ctcgctatcg attatagctt gtaggtttgt tccgcctcct gaaacaagga cggcaagacg
     6781 cagagcttta ttttgcattt tttctctctt catgccctgc cccactttag tcttcaaaga
     6841 caagcgcttc ttcttggctt aaaaaagcag gtttttcgag tgcttcaacg cgaccgatta
     6901 tacgcgcttc ttcgccatgc tgcttaaaag tctcgattat agatgcagct ttttttgcat
     6961 cgactgcaag cacaaaacca atacccatat tgaaggtatg gtacattcta tcggaatttg
     7021 cgccacgttt tacaagctca tcaaagatag gaagtatagt ccaggatttc ttttttatca
     7081 ctacacgatg caaaaactgt ccttcattat ttttggggaa catgcgagga atattttcat
     7141 aaaaaccacc gccggttacg tgcaccatgc catgcaccgc ctttttgtat ttttctaaga
     7201 cagcaagaac aggttttaca taaatctttg ttggctccaa aagcacttca ccgattgttg
     7261 ttccttcaaa tgcggtgtcg aagtcgctta ccagtttacg cacgagagaa aagccatttg
     7321 aatgaactcc actcgacgcc aaaccgatga ggacatcacc cgcctgtatt ttatccccgg
     7381 taacaatttt tttcttatcg ataatgccga ctccaaagcc agctatgtcg tacttccctt
     7441 cgtcgtaaaa acctggcatc tcagcagttt ctccaccgag caaggcgcaa cccgtttgta
     7501 agcagccctc gctaatgccc tttaccaagt cggctgctat ctctgagtcg agttttccac
     7561 aggcaatata atctaaaaaa tacagcggtt ttgcccctgt gcaaagaata tcgttgacac
     7621 tcatggcaac acaatcaata ccgactgtat cgtatttttt catagcaaag gcaatatcga
     7681 gttttgttcc cacgccatct gtcccactca ccatcacagg attttttatc cccttgggaa
     7741 cagcaaacat accagcaaaa ctaccaagcc cgttgagaac tcccggaatc atcgtcgcct
     7801 ttgcaaaatc cttgtatttt gaaacagcct tatagccttc ttctatatct acaccagcat
     7861 ctttataatc aaccatatct atccaccttt tctattttcc tgtatccaca gtctttgtct
     7921 tattcctatc gtttttagtt gccatttgta tttagtcctg ccatgggaac tgcaatcgga
     7981 tacttgccag taaaacaacc cttgcaatag ccatagtttt tttcatcgat acccttcaaa
     8041 gcttcaagca ttccttcaat cgaaataaaa gcaagggaat cggcgcctat ttgctccctc
     8101 gttttttcaa ggctaaagtt tgaagaaata aggtcctggc gactcggcgt gtcaatgcca
     8161 aaataacagg gaaattttac cggcggagat gaaatgcgaa aatgcacttc ctttgcccct
     8221 gcgcgacgca aaatctcgat gagccgtcga ctcgtcgtgc cgcgaacaat cgaatcatcg
     8281 ataacgacaa cgcgttttcc ttcaacatcg ctttttaaga cattgagttt tacaaagaca
     8341 atattttctc ttgagctttg tgcaggtgca ataaaggagc gcccgatgta tttatttttt
     8401 acaatgccca tcgcataggg aatgccgctc gcatttgcat agcccatggc agctcccaag
     8461 cctgaatcag gaaccccaac aacgacgtca gcgtcgacat ggctttcctt cgcaagcacc
     8521 gcccccattc gaagacgagc ttcttgcaca gcgataccgt ccataatgga atctggacgg
     8581 gcaaaataaa tatattcaaa aacgcaggtg tgttttgccg tttgctcacc aaaatcaaag
     8641 gagagcaccc cgtccttatt gattataata atttcaccgg ggcgaatatc gcgaacgagc
     8701 tccgcaccaa tggcatcgag ggcagagctt tcacttgcga gaacccagcc attttcaagt
     8761 tgccccaaac aaagcggacg aataccgtga gtgtcgcgtg cgccgatgag agaattttct
     8821 gtcatcacta caagggaaaa tgaacccttt atcatttgaa tcgtgtcggt taaagccctg
     8881 tccaagccct ttttataact ttttgctata agctttataa taacttcaga atcgctggta
     8941 gaaacaaagg ttgaacccgt ttcttcgaga aattctttta tttgctcata atttacgaga
     9001 cttccgttat gagaaaccgc aatagttccc agctttgaac gattgacaaa aggttgcaca
     9061 ttttctatat gaacattatt tgttgtttca tataaaacat gaccgcaggc tatatttccc
     9121 gagagccctt gtaatttatt ttctttgaaa acttctgcaa caagcccaag cccgcgaatt
     9181 tgttctactt cgccattatt agaaacagca attcctgcag attcttgccc acggtgttgc
     9241 aaggcatgta aaccgtaata ggccagcttt gccgcatttt gcccgagcag ctcttttgat
     9301 tgtatctttg ttttggtttt gctatcagcc gctatctcag tattttcaga gcagccttca
     9361 gtatttagaa aaataccgat aacgccacat ttatccttga gcttatcttc tgcattttca
     9421 tcgattatat gcgaaagcat atttttttta aaatcacctt gatttatatt catttttaca
     9481 tcctactcgt acatcacatt caactaaaaa aggccctaca aatcgacacc acaagcacca
     9541 tttagcaaag cttcatccac cagttttttt cgaaataaaa tgagtttttc ttttaagtct
     9601 gcatcagaaa gtgcgagtat ttgaagcgca agataggccg cattttctgc attgtttata
     9661 ccaacagtcg ccactggaat ttgctttggc atttgtacta tggaaaaaag agcatccatg
     9721 ccagctaagc cattcgattg cggactcctg cattcaagcg gaatacctat gacagggaga
     9781 atcgtatggg aggcgatgac accgggaaga tgtgcggcga gaccagcacc tgcaataatt
     9841 acttgacaag cttcagcctc aacttcacga atgcgttctt tgaggagatc tccacttcta
     9901 tgtgctgata aaacaaaggc tttgtaggaa acatcgaatg cttttaaaac atccccagcc
     9961 tttttcataa tttctgtgtc ggattttgaa ccgaaaaata atgcgacttt catttacaga
    10021 tattaacata aaaaaagcct ttcttcaaga cacaggggag aatccaagtg taaggcaata
    10081 aaaatgaatt tgcaagataa gatgttgaca agtaagtctt tggctagttt aatagatatg
    10141 atacgataaa atatatgaga agaggggtta aatgaatgat actagacaat gaaaacgaga
    10201 atttgaaagt ccatgagtgg attcaagaat acaccgagca aggaagctta gatattataa
    10261 cagggtattt taccataggt gctctggctt ggctttctaa aactgtaaat gataaaatta
    10321 cagacttcaa acttattttg ggtgatattg taaatattga ttccgtagaa aatcgtcccc
    10381 tcgatctctt aaacgaaaat attagcattg aagcttcttt aaagctaagt tcacttgcaa
    10441 aagaagctgt caatttttta aaacaagata aggttgtggc aaaaacacta gaacccaatt
    10501 tctgccatgc aaaaacctat ttgtttaatc caaataaaaa tgatgataga aacaagtact
    10561 ttatttctgg aagctcaaac cttaccgaag ctggtattgg attaaaacaa acaaacaatg
    10621 tagagttaaa tatcgcagaa acaggtaata acaatcaata caaagaacta agtacgtggt
    10681 ttcacgaaat atggaacaaa ccacaagcac acaaaaagaa aaccctaata gattctgaag
    10741 gtaagaaaac aagcgtagat tttaaaaact atctgattgg cgaaatagaa caaattttta
    10801 taaaatattc tccgagagag ctttattata aaacgctctt tgaattattt ggcaaccaat
    10861 tgctagaaac agaaagtgac cccaacttta accgacaaat cggacgttta gaaaattctg
    10921 ccatttacaa cacactgtat gagtttcaaa aaaaaggcgt tttgagtttg attcgaatgc
    10981 tgcaaaaata taacggagct attttggcag atgctgttgg actgggaaaa acatggagtg
    11041 ccttggcggt gattaaattt tttcaactcc aaggaagaga aacaatgttg ctctgcccca
    11101 aaaaattaga acataattgg caaagatatt tgagaaacca agactcacgc tttgaaaaag
    11161 accagtttga tttctttata cgctttcaca cagatatgca cgaagacaga atggaaaaat
    11221 acacagatcg caatgacaaa tactttacaa atgataaacc aaagttaata gtcattgatg
    11281 aaagccataa cttgcgaaat gataaatcga accgttacaa attttttctc gaagaaattt
    11341 tgaaaaaaaa tgaagatata aaagttttac ttctttctgc cactcccata aacaattcat
    11401 taacagatat tcgaaaccag tttaagttga tggtgcaagg aagagtcgat ggatatgaag
    11461 actcattggg cattcgcaat ttggattaca cttttcgagt ggcacaaaac accttcaatg
    11521 aatggcggaa agatgacaaa ccacgaatcg ctgagtttat aaaaaaactc cccgaaaatt
    11581 tctttacact tacagattca ttgattgttg cacgtactcg aaaaatgatt gaaggacagc
    11641 aaacgggatt aacttttccc ctaaaagcaa aacctgaaaa tcattttgtt acacctagcc
    11701 aactaggaaa ctttgaatct tttgaagagt tgtttgacca ttttccgcca atgctttcgg
    11761 ggtatcagcc ttcattttat ttagatgaag atgaaacaga tatacttcat gacgaaaaac
    11821 aaagagaccg ttttcttgtt aaaatgatgt atattctgat ggtaaaacgc ctggaatcct
    11881 catggtattc attttattcc accatagaaa aaataaaaga ccatcannnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnncaac ctccaaaaat ttaaagaaaa aattgaaagt
    12061 gaaaccgttc ttccgagaaa tcaagaatct gcagatgata aactgcaaac tttgataaaa
    12121 gaaatcaatg aaaaaagaaa attgggcgaa aacaatggaa acaaaaaagt tgttatcttt
    12181 acggtttatc gagacacagc tctgtacctt tataaccaat taaaaagtcg cggttttgat
    12241 aagttggcaa tggtctctgg aacaggttct ataagtagcg attcaaaaga agagactaaa
    12301 aactttgaat ctattttgga acgttttgct ccatatacaa aactatatat ggaaaaagaa
    12361 tgggatttta agagcgagaa aaaaggaatc gaagcctatc atgaatggat ttcgtggata
    12421 gctcaaaacc acaaaaaaac ctatgaaaaa gttcaaaatc caattgatat cttaattgct
    12481 accgatgcat taagtgaagg acaaaacctc caagatgccg atatggttat aaattacgat
    12541 atccattgga acccggtgcg tgttattcaa cgaatggggc gtatcgatcg attgggaagt
    12601 ccgaataaaa aaatatttgg aattaatttt tggccatcta acaacattaa cagctatcta
    12661 aatcttcaag gaagagttga agagcggatg gcaacaatga agttggcagg atccgaagtc
    12721 gatgaaaagt tttctaagag ttttaaagaa atgtcccatg acgaatcttt cgaccaaaaa
    12781 atgaataatc gcatgttgga acaaatgaaa acgacctttg acgatttgga aggagaaaag
    12841 gcatttggct tcgatgacct ttccctggaa ggctataggc aagatttagt ggaagaattc
    12901 aacagagaca aagcaaaata ccaaaaaatg cccaaaggcg tctatactgg attcaaggca
    12961 gatagcgcta tttgcgcaga aaatggctta attgctgtat tgggctatcc tgcaaaagct
    13021 ccaaaaagcc cacaacacga atacaccaaa tataacctta tatatataaa cagcgaaggc
    13081 aaatcagtat tgctcaatca aaaagaaatc ttggatgcct tgacacttca caaagaaaaa
    13141 gaccgatttg tgcctgaaaa gatagacagt ggagatgaag aggctattca agacttggca
    13201 ggttctctaa aatcttggtt agaaagtcaa gccgtagaag aagtagcgca agaagacggt
    13261 acaacaaaaa aacaaatggg aatagaagca aaaaatatcc tagcaaaact gaggaaggga
    13321 gataaagacg ctgtaagcaa agtaaaacaa aatatcaaag tagaagaaaa attccaaatg
    13381 gaaaactttg acctaattac ttggtttttg gtgacggtct aatacaaatg aaaatgaaat
    13441 ttgacgatct tatagtacag attcaagaag taagcaaaaa tttacagcaa caagctgtaa
    13501 aagcggtgaa tgtatcactc actttgcgaa attggttaat tggtttttat attgtagaat
    13561 ttgaacaaaa cggagaggat agagctcagt acggtgatcg attgttagaa agaattgctg
    13621 aaacaattca aattaaaggg ctgggtatca ccaacttaaa atggtgtagg attttttatc
    13681 agaattatcc gcagataatt cagccaataa ctcaaatgtt cgacaaccca atcttcctcc
    13741 caattcgtca gtcaataact gacgaattac aaagtaccaa aaatcagatg cataagttcc
    13801 atcctttgcc aattgacgaa tgcaataaat ctcaaaaaca catacaacat ttattaaacc
    13861 actgctcttt tacgcatttt atagagctga taaaaataga tgacgcaatt aaaagaaagt
    13921 tttatgaaat gttaattcta aaaacaactc cctcgataaa agaactaaaa aggagcattg
    13981 aaagtttgac ttacgaacgc tttggactct cagagaataa agaaaaagct ttgcagaaga
    14041 taattaataa aattcaacct caaagtaata cagatttaat caagtcacat tatttttttg
    14101 aatttcttaa catttcccac cccgaactaa tcgaagaaag tgatttagaa caagctctga
    14161 ttacgcatct tcaacaattc attacagaac ttggtaatgg attttgcttt gaaaatagac
    14221 aaaaacgtat cctcattgga gatgaatatt attttattga tttagtcttt tatcatcgga
    14281 ttcttaaatg tcatgtactt attgaactga aaactgaagc agttaaacac gaacatatag
    14341 gacaactaaa aacctatttg aattactaca aaaaaaatat ccaagagcca aacgacaatc
    14401 caccagtcgc catattatta attacagagc aaaacaaagc cctcgtggaa tatgcaatag
    14461 cagacgcaga caaagatcta tttgtaagca aataccaatt acaactccct cgaaaagaag
    14521 aaatagaaaa atttattaca aatgaattga aaaggttaaa aaatgagtaa actagaaaaa
    14581 tttaaaacag attcttttat cgaagctact caatcttttt ttaaagattt gggcttgcca
    14641 attcttaaca taacagaaga gccaacttcc gcaaaagaaa ctctcaaaga tacttataaa
    14701 gagaacgaaa gctttcagct tatcgatgat gtttattttg taggaatggt agacgatgct
    14761 gcttttgaaa acaaaacccc tttaaaccca agtgaaatca aaaatgaaat cgaacgtgat
    14821 tacgatggta ttttgatttt tgcccttacc cttaacaaaa gacccaatga tttacttccc
    14881 acccgttcgc agctcgcaga aatatccaga gcatttaacc gtgagtttta ttatactcca
    14941 gttgtaattg tgtttaaata caataattat ctcgcatttg caaatacaga acgattaaaa
    15001 tacaagcaag agtggcgaga aggggaaaaa gcaggtaaag ttagtttact gcgagatatc
    15061 tatatagaaa aaccacattc tggtcatgaa cgcattttat ctgaactgga aattccatcg
    15121 agtgggacca aaaaaataaa cacctttgct acactctacg cccactggca agaagttttt
    15181 agcgtaaatt tactcaataa aaaattctac caagaacttt ctaactggta tttttgggca
    15241 atcaaaaatg tagttttccc aagtgaacct actatcgaag atgctcatag aaaaaatgct
    15301 aaattagaag atctaataca cgagcatcgt gcaacaaatg ttatccgcat gctcacgcga
    15361 ctcctttttg tatggtttat aaaagagaaa aaactcattc ccgaagaatt atttgaactt
    15421 gaaagcttac aaaaagatat tctaaacgaa atagaaccgt tttacgaaaa tggattattt
    15481 accgaaagca acaaagacag cgtctattat aaagctattt tacaaaacct attttttgca
    15541 acactcaact gcccaatcct gtcagatgac ttagacaaaa gatttagagg atttcgaaga
    15601 gaaaagaatt atggagatga ctttggaaac gattggctct atcgatacaa aaaatatttt
    15661 aaaaaccctg acatattttt agaactgatg aactctactg ttccattttt aaatggtgga
    15721 ttatttgaat gtttagatga taaaccgcag aaaatctaca tcgatggttt ttccgataac
    15781 ttagctaaag gtgagcagct tatagtaccc gactatcttt tctttggtct cgaagaagaa
    15841 gttgatttaa gcgatgtcgt tggcataagt gatgcaaaaa caaaacaatc tgccgttaaa
    15901 ggtcttataa atatcttaaa gtcctacaag tttacgataa ctgaaaacac ccctatcgaa
    15961 gaagatgtag cacttgaccc agagcttttg ggaaaagtat ttgaaaacct actagcaagt
    16021 tataatcccg aaaccaaaac aacggcacga aagcaaactg gctcctttta tacaccgaga
    16081 gaaattgtaa actatatggt ggatgaaagc ttgattgcct acttagaaaa tgcaattaaa
    16141 gattgggaag gattaaacga aaaagaaatc gatgaaaagc tccaccaact cacttctttt
    16201 gatactgcag aagtatttgc cgataaaccc gatttgcaaa aaaagattgt tatggcattg
    16261 agtacttgca ctattctaga cccagcctgt ggctcgggtg cctttcccat gggcatttta
    16321 cagaaaatgg tacatatcct acaaaagctc gattcggata ataaaatatg gtatgatgtt
    16381 caattagaaa aagcagaaca agagagtaaa gatgctttta aaatcgataa caaggaagat
    16441 cgtggagagc gactacaaga aatcaatgaa gtttttgacc gaagcatcaa cgatccagat
    16501 tatgctcgta aactcttttt gatagaaaac tgtatctatg gagtagatat tcagcctatc
    16561 gcaacacaaa tatccaagct acgatttttt atctccttga taaccgatca gaaagtaagt
    16621 cctgaaaaaa cgaactttgg catacgtccc ttacccaacc ttgaaacaaa atttgttgct
    16681 gccaatacgc ttataggaat agaaaaagaa agtggtttgt tttttacaga agatatacaa
    16741 gaactagaaa aaaagttaaa agtaatccga agtaaattgt ttaatgcgag aactaaagaa
    16801 actaaaaata aatacagaaa aaaagacgaa gagctacgaa cagaaattgc aaaaatactc
    16861 aaaaacaatg gcctaccttt ggatagcgca gaaaaactag ccacttggga cccctacgac
    16921 caaaacgctt cttccccctt ctttgatccc gaatggatgt tcgacataga agacggcttt
    16981 gatgtggtga taggaaatcc accgtatgtg caattgcaaa agaatactgg tgctttagct
    17041 aaagaatttg aaaatgcaaa cttcgaaact tttacacgaa caggagatat ttactcctta
    17101 ttttacgaaa aaggtattaa tgtattaaag caaaatggac atttaaccta cattacctca
    17161 aacaaatgga tgagagctgg ttatggaaaa agtacacgcc aatattttac aaaatttaat
    17221 cccataaaat taattgattt agggagtggt gtttttgaaa gtgcaacagt agatacaaac
    17281 atcttgatta ttcaaaatgc aaaaaacaaa aacaaatgtt tagcggtaga tctttcaaaa
    17341 acaaaagaaa atgtagattt aaataatttg aattatgaaa tattgaaaaa tcttagcagt
    17401 gaaagttgga caatagccaa taaaatcgag caaagtataa aagccaaaat tgaaagaatt
    17461 ggaactcctt taaaagaatg ggatattcaa atttatcgag gggttttaac tggctacaat
    17521 gaagctttta ttatcgatgg agaaacaaaa gataaactca tagcacaaga tccgaaaagt
    17581 gcggagatta ttaaacctgt tttgagaggt aaggatataa aaagatataa agctgaattt
    17641 gctgataaat ggttgata
//
LOCUS       DAOSSW010000031        16924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_3494_length_16924_cov_7.256980, whole genome shotgun sequence.
ACCESSION   DAOSSW010000031 DAOSSW010000000
VERSION     DAOSSW010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 16924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16924
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3494_length_16924_cov_7.256980"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2194)
                     /locus_tag="PKW26_04140"
     CDS             complement(<1..2194)
                     /locus_tag="PKW26_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOQ92790.1"
                     /translation="MTLENSQAEKLIRSKTNEEQFNAVQMLKNAVVAAGAGSGKTRVI
                     SYRYAYLISVRGYKVSEILTLTFTDKAATQMHEKIYEVLKEIAEHEVVDASIQRAKQA
                     LEDFQDARIQTLDSYASSIVKNQGRLYGLSPDFMIDLEKTREEAEREALQFALEHKEN
                     QYLKNFIGTQNFESKAKSLFAQTIDTQSSLSKAINFEKDFEKQKKHILHEYDIITKKT
                     KDFFQTAFSIFNEIDDKKEMTSAAYKNIALFYQDNTELIFPPVDSIASFFHGENKELK
                     QELYACLKVFYAFQKIDNRTKKEGPLLDIKNIIRESKILYAELNSIILYVFNASTTQE
                     LYKLLSLFQEKWNTKKRRSLILSYKDISDLALEILTHHYETRESEKQSYKAIMIDEFQ
                     DNNLEQKNLLYLLAEKPVLKKDFIPEIPSENQLEPEKLFFVGDEKQSIYRFRGADVSV
                     FRGLQEAFDSSIQLSTNFRSHPSLITSFNSLFGGADYPQSMKSFIQNQTGERILDVDK
                     TRASIFLKPSHIIEGEEFPLFEAEYHGVRAQNAKEKLIQSEDEIQAAIHFCFHVSEGE
                     ETEEDTGESEEKNKATKNTSSNEDETLTKDEAEAFFTANKISELIKNSEGELSFSDFV
                     LLFRSYTKQNYYEKYFRNLGIPYISESIRSFFSDAPVNDIFSLLRLLVFPYDSLAYAS
                     FFHSPFVRLSLEGVNTCIYFLKNKTESETPNLFDTGIVESLNEEDKERF"
     gene            complement(2215..5196)
                     /locus_tag="PKW26_04145"
     CDS             complement(2215..5196)
                     /locus_tag="PKW26_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOQ92791.1"
                     /translation="MKNQITEKKSEYQIITDILSKTIKNEDAYFVFPTEITAKAWAEK
                     SLFLTDYKAVELDRFIAWDTFKGTAIRSVKQDRASIPSLLRKLFVENLLENTEEPFFK
                     ALIPEQWADLAPSFSSWISSLLPQLARFKAHLVQNLSEGSLNFENSNFEKQSKEILEK
                     IAKTEGDAENRDLLKLENTYAQFLENNGLFEPAWEKPPFHNDGKSYYVFFADITSDYA
                     EYASILENAENVKLIASPSKESVLENLQVIAYENSREEIRKTALFIEELLDTGYAASE
                     IAIHLPEMEELAPYLMRELKLRDIGFQLRAGKALSLYGAGSFFSSMKRLVDEEFSFDS
                     LKQILLNKNLPWKTSVETLINKLLNFGVKNNCLCSYKKKTVLVDLWEEAFKIDYDESL
                     VLFYRSLKNSATEFAKAKSFEALKKNYFEFRSTFFDMEICSEESDLILSRCITELLNC
                     IELEKSYPSVQVKNPLAFFCSHLDTSEYLPQQKTKGVAIFPYKAAAASPYRQHIILNA
                     GQKQVSVLHQYLSFLLKTKRQKLGIEDSNVSEKMLALYSMGQVRFSYSEKGLSHYSLP
                     HTDFQKILAPEKIEGKDIYLLEQNIFYTQGETESDKKTAYPQADFLYKTQKDGFANWI
                     KARPQEEKIKTFSLDSESYEKFLTSVSDRETNMLRVSATKLRDFFFCPLFFLFSRHYK
                     LKNERLETSLLAPVYLGQLYHAILKTFFIYYKDKDEALPEPPKTKNAFQENKTSEAYY
                     PVLESAFKTIMDGFPKSVFPNENLSPLSQEIILNQAPAFFKTLDEAVKIFLSYFYNSR
                     VLETEAWHESKEEKYLLLGSIDLVVEKPNGEIWIIDFKTSSPPSKTDCLADEEGRLEN
                     FQLPLYIRLFEKTKNKRVDGAAFFTINNPAISPLIGYIEDNEGKNKKPYYAKNIVHRN
                     PQNEESNTDKAEVNFNETMQALNKHIEDYVEKISNNIFKLDESIPFTQCAACEYKKLC
                     RTTYRVSGDKKASRGLIDLRF"
     gene            5267..>6072
                     /locus_tag="PKW26_04150"
     CDS             5267..>6072
                     /locus_tag="PKW26_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HOQ92792.1"
                     /translation="MKMYSVLRGKALPHGATIHKDGVNFSVFSRHATAIWLDLFEKPE
                     DDLPFQSIKFDEKINKTGDVWHLFIPGLKAPAFYLYRVDGPFEPHNGHRFNKNNYLLD
                     PYARELTDVSVFKQLHDGFFIPKDSYDIERQKPQTAKKFPKSVVVDDEFDWQGDTPLN
                     LPLRQSVIYETHLKGFTAHESSHVKHPGTYRGLIEKIPYLKELGITSVELLPVQEFDE
                     WENTNINPKTGERLKNYWGYSTIAFFAPKIGFSSDKSPGGAVREFKEMVR"
     assembly_gap    6073..6172
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6173..6934
                     /locus_tag="PKW26_04155"
     CDS             <6173..6934
                     /locus_tag="PKW26_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HOQ92793.1"
                     /translation="FITSHDGFTLNDLVSYNGKHNEENGENNRDGSDHNVSYNYGFEG
                     PTQNRDIEQIRNQQIKNFLCSLLFSQGTPMLLAGDEMRRTQNGNNNAYCQDNALTWID
                     WSLKDTHSTLFRFTKQAIAFRLSHPAFRRPEFFQGHDHSYNAIPDIFWYDEFGEAIDW
                     GSANHFLAFYLDGRHAEVFADIDDNDFYVMLNSSTKDKTVVVPPLQNGRKWYRVIDTA
                     LPSPHDFTPPGDEEILQNQAKYVQKARSFSLLLSK"
     gene            7015..9462
                     /locus_tag="PKW26_04160"
     CDS             7015..9462
                     /locus_tag="PKW26_04160"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOQ92794.1"
                     /translation="MQFDKHKFKENLSARLRRQYGKDISQASFHNLYDAVSASTLEFI
                     MEDWMQTRKTFETKPVKQMSYLSAEFLMGRALNNNLINTGFLEPVKEVLEEMGIDYAT
                     IEEQESDAGLGNGGLGRLAACFLDSLATLDYPGHGYGIRYEYGMFEQRIENGFQVEYP
                     DNWLKHRDPWEIKRSDLAVEVEFGGRVAMKKGSNGSDVFYLEDAERVTATPFDMPIVG
                     YGTKTVNTLRLWQATSPNGFDLQLFNDMQYHRSVEKQNDAENISRVLYPNDSGPSGKT
                     LRLKQQYFFSSASLQDLLNAFVKEHGKNFELFPEYNVIQLNDTHPVVAIPEMMRLLID
                     VYRLGWDEAYSIVEKTFAYTNHTILSEALEKWPIATFKELLPRVYQIIEEMHRRFSIN
                     LKEAFPEDYARQNRMAIISNGMVFMAHLAISTCFSVNGVAALHTEILKTKELKDWADF
                     YPQKFNNKTNGVTQRRWLLSSNPRLAKFISARIGDGWQKDLSLLKGLEKFVDDDASLT
                     ELISIKQKNKEEFAEYIKVTQNEFLDPQSIFDVQVKRLHEYKRQLLNILHVMYLYNKI
                     LEDESYKPLPRTFIFGAKAASGYRRAKTIIKLITTVAERINNDKRVKGLLRVVFLENY
                     RVSLAEKLFPASDVSEQISTAGKEASGTGNMKFMMNGALTIGTLDGANIEIVEEAGDE
                     NAFIFGLTADEIHAIEKEHSYQPQRYLDSNPELAKVVDQLIDGTYDPTNHLFKELHDS
                     LLWGVDGQRSDVYYVLADFEAYAKAHEKVLEAYQDQKGWAKKALLNIARSGKFSSDRT
                     IEDYVSDIWKIEKLLVE"
     gene            9465..>10328
                     /locus_tag="PKW26_04165"
     CDS             9465..>10328
                     /locus_tag="PKW26_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOQ92795.1"
                     /translation="MEQVGAKTLLASLGLMLVTLLWGLSFAVVKNSLDLVPPVYMMAF
                     RFSIASLALLLVFPKKLSAITKETLKKGFVLGIFLALGYALQTIGVVYTSAGKNAFIT
                     TTYVIFVPFIYWFFTKHKPGFFSIIPAFVGLLGIALLSLQDDLSVNKGDILTLLCGIS
                     YAFHMVFIALFTKDDDPILLNLVQIFFVTVFSWLLAPFYDGAFPLLAIQDPATIWSML
                     FLGIFCTMLAFFLQTLCQKFVPPGTASLIMSLEAVFGVFFAWLILGEILSLRMAFGAL
                     LVFVAILLSQKK"
     assembly_gap    10329..10428
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10494..11312
                     /locus_tag="PKW26_04170"
     CDS             10494..11312
                     /locus_tag="PKW26_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ92796.1"
                     /translation="MKKNLRIFAIIALVVLAFAFTACAKTTKSDGLTIKEGVLQIAME
                     IGYPPFEYFDTDGKTPIGFDVQLGKAIAEELGLQVAYVDTAWDGIFAGLKINKYDVVI
                     SAVTITDERLKEFNFTKPYIGNAQSLVLLKNTELSAKHPEELGGLRVAYQAETTSDIY
                     MTKLKAEKGVNYKEFEYDKVMNCFDDLKNGRVDAIVCDSLVAVDYVADANAPYEIVWL
                     GEADEFFGVCIKKGNDALTEKIDLALDKLFAEGKIQAISKEIFGSDIVSATRAN"
     gene            11355..12014
                     /locus_tag="PKW26_04175"
     CDS             11355..12014
                     /locus_tag="PKW26_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOQ92797.1"
                     /translation="MEKLQTILNWIPPLLEGAVVTISLTLLSVIAGLIGSLFLALGKM
                     SKNKALNRASSAYVFFFRGTPLLMQLYFIYYGLPQIAPWLTINNRFIAAFIAFGLNSA
                     AYCAEIIRAAIQSIDKGQFEAARALGMSYGQTMRLIIIPQSFRRLIPPVGNEFIMVLK
                     DASLVSIIALSDITKITRSISSSTGSALVYIPAMILYLIITGIFTLVFNKLEKKYSVY
                     E"
     gene            12051..12803
                     /locus_tag="PKW26_04180"
     CDS             12051..12803
                     /locus_tag="PKW26_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOQ92798.1"
                     /translation="MIKTINVCKNFGSLEVLRDVSLTVDQKEVVCIVGPSGAGKSTYL
                     RSLNRLEKITSGQVYIEDVLMFDAEHGVNKVKMPAKERAERLLELGMVFQRFNLFPHK
                     TVLENVMVSPIHVKKQSLSYAEEKAMALLERVGLSDKLHEYPAKLSGGQQQRVAIARA
                     LAMEPKIMLFDEPTSSLDPELVGEVLGVMKQLALDGMTMLVVTHELGFAREVANRVVF
                     MYEGRVLEDASPDEIFKNPKHERTKQFIKSVL"
     gene            12822..13379
                     /locus_tag="PKW26_04185"
     CDS             12822..13379
                     /locus_tag="PKW26_04185"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOQ92799.1"
                     /translation="MDIFTYTLIVVFFIFAAILSFIDIKHQKIPRFLSFSFIGSIYGL
                     RILGTLLLFLISNGESETGRLSFSAFLFALYPPTLGLILGLAVFYAVRVVSKLKLGLA
                     DVWFSGAMGTFLGYLGWYAAIAISCLFALSWIFSLAVLRKIRAKHEGQKEISLLKTFR
                     QIQIPFIPFLASGSLCALLVSSLTR"
     gene            complement(13393..14592)
                     /locus_tag="PKW26_04190"
     CDS             complement(13393..14592)
                     /locus_tag="PKW26_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ92800.1"
                     /translation="MATLLLIIIYVSFISLGLPDGMLGAAWPVMRLEFAVPLGSAGII
                     SMIISLGTVVSSLFSAKLIKKFGTGMISLVSVFLTALALLLFSFAQSLYPLFLFAIPL
                     GIGAGAIDSALNEYVAEHYEASHMNFLHSFWGIGALSGPVIMSLFLQKNGSWNSAYFL
                     VAIIQFALTLALFFALPLWNHKSLQKKEHPDADIFKDEPKQKEPLKKVLTQRGVKGVL
                     ISFMLYTGIEATMGLWGASYLKDSLFFSAERAALWTSLFYTGIMAGRFISGFLAMRLS
                     SKKLLKIGTSLISLGSLFLLLSLIQSLPHEIALIGFILVGLGCAPVFPGMLHETPRRF
                     GKEHAQSIMGFQMAVAYTSTTILPPLFGLIASFLGTWLLPFTIVLYGFVLYILNKRSF
                     RVFNTSA"
     gene            complement(14644..16290)
                     /locus_tag="PKW26_04195"
     CDS             complement(14644..16290)
                     /locus_tag="PKW26_04195"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HOQ92801.1"
                     /translation="MNKKTYIIGHRNPDTDSVVSAASYAVLKESLGGKDFFAARAGKM
                     TAQSEYIFNRFKVPVPEYLSDLLPKVGFYINEAYQTVHENTSLWNAVAKMEECNSKVL
                     SVIDSEGRYKSFLHYNAFAQNVLKILNPEKKTSVTTSIKLICDTLGAQPLHTKDENRI
                     SNSAILIAASQFDTFCEKFNEHLPENLIVITGDRADIQEYCIERGVKVIVLTAGSVLK
                     KDLQAKAEKKDVCALISPYDTSSTAMLIVYSTPVSAISDTDIKPVNKNDLVRSIKPIL
                     QNSPSRCLPVINDEKKLIGIISESDLIQEANIEVIMVDHNELSQAIEGIEHYKIKEII
                     DHHRLGNLNTRDPITFINKPVGATSTLIATLFMENKVPMPKPIASILLAGILADTLIL
                     QSATTTDIDRDVAEYLSNITGLDIDELGSDIMSASSRIGKRSANDVIQQDMKEYTEGE
                     HSFTVSQIEVDNPEEIMERKEEFLFALETAKKAKNLLFSALMVTDVTKLTSFLLISGD
                     ASFIQSITFPKQEQDMYVLKDIVSRKKQLIPLLSEQVEKY"
     gene            complement(16283..>16924)
                     /locus_tag="PKW26_04200"
     CDS             complement(16283..>16924)
                     /locus_tag="PKW26_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92802.1"
                     /translation="ISPVKHYTAIVDGTAPELRYLIHDAYYIDENQNVYFTSDTSLIV
                     WGEDIGSGTENLFVRINDSEYIDFIDENMIYLNETYEDGSYQLESYLVDRVGNTSPIF
                     SQLIYLDNTAPEVEIEIDPNAIVLNDRIFIDPTTVLSFVANDEGSGVKDIFVSINGAE
                     FASVQGDYVVPEKGENTVRFYAVDNLGNKSDVKEVSFSNALSLPETELYLEIE"
BASE COUNT         4838 a         3069 c         3429 g         5388 t
ORIGIN      
        1 ggaatcgttc cttgtcttct tcgtttaaag attcgactat tcctgtgtca aagagatttg
       61 gtgtttcgct ttcggtttta ttttttagga aatatataca ggtgttgacg ccttctaaac
      121 taagtcgtac aaagggggag tgaaaaaaac ttgcataggc gagcgagtca tagggaaaaa
      181 ccaaaagtcg caaaagtgaa aaaatatcat ttacaggagc gtcagagaaa aaacttcgta
      241 tgctttcact gatatagggt atgccaagat ttctaaagta tttttcgtaa tagttttgtt
      301 ttgtatagct tcgaaataag agaacaaaat cggaaaagct aagttcgcct tctgagtttt
      361 ttattagttc agatatttta tttgcggtaa aaaatgcttc agcctcgtcc tttgtcaagg
      421 tttcatcttc attgcttgat gtgtttttcg tagccttgtt tttttcttca ctctcacctg
      481 tatcctcttc agtttcttca ccttcgctta catgaaaaca aaaatggatg gctgcttgta
      541 tttcatcttc gctttgtatc agtttttctt tggcattttg ggcgcgaact ccatgatact
      601 cggcttcgaa gaggggaaat tcttctcctt cgataatatg tgacggcttg agaaaaatag
      661 aagccctcgt cttgtctaca tcgaggattc tttctcctgt ttggttttga ataaaagatt
      721 tcatgctctg ggggtagtct gccccgccaa agagtgaatt aaagcttgta atgagagagg
      781 gatgagagcg gaagttcgtt gaaagctgaa tgcttgagtc aaaggcttct tgtaagcctc
      841 ggaaaacgga aacatccgcg ccacgaaatc gatatatcga ttgtttttca tcacccacaa
      901 aaaaaagttt ttctggttca agctgatttt ctgaaggtat ctcaggtata aaatcttttt
      961 tcaaaacagg tttttctgct aagagataga gaaggttttt ttgctcgagg ttattgtctt
     1021 ggaattcgtc aatcataatt gccttgtagc tttgtttttc ggactcacgt gtttcataat
     1081 gatgtgttaa aatctcaagg gcaagatctg aaatatcttt gtagctgagg ataagtgagc
     1141 gtcttttttt tgtattccat ttttcttgaa aaagagaaag tagcttgtag agctcttggg
     1201 tagtagacgc attgaaaaca taaagaatga tactgttcaa ttctgcataa agaatttttg
     1261 actcgcggat gatattttta atgtctaaaa gtggtccctc tttttttgtt cggttatcta
     1321 ttttttgaaa agcgtagaaa actttaagac aagcatatag ttcttgcttt agttctttgt
     1381 tttcgccgtg aaaaaaagaa gctatagaat ctacaggtgg gaaaataagc tctgtattgt
     1441 cttggtaaaa aagagcaatg tttttatagg ctgcagaagt catttctttt ttatcatcta
     1501 tttcattaaa tatactgaaa gctgtttgaa aaaaatcttt tgtttttttt gttattatgt
     1561 cgtattcatg aaggatgtgt tttttttgtt tttcaaaatc tttttcgaag tttatagcct
     1621 tgcttaagct actctgtgta tcgatggttt gggcaaaaag agattttgcc tttgattcaa
     1681 agttctgtgt acctataaag ttttttaggt attgattttc tttgtgctcc agagcaaatt
     1741 gtaaggcttc tcgttccgcc tcttcccttg ttttttcaag gtcaatcata aaatcaggac
     1801 tcaatccgta taggcgtcct tgatttttta caatggatga tgcataggag tcgagtgttt
     1861 gaatgcgtgc gtcttgaaaa tcttctaatg cttgctttgc tctttgaata gatgcatcta
     1921 ctacttcgtg ttctgcaatt tctttaagca cttcgtaaat tttttcatgc atctgtgttg
     1981 cagccttgtc ggtaaaggta agcgttaaaa tctcactcac cttgtaacct cgcacagata
     2041 taagataggc ataacgatag gaaataacac gcgtttttcc gctccctgca cctgcagcta
     2101 cgactgcatt cttgagcatt tgtactgcat taaattgttc ttcgtttgtc ttgctgcgta
     2161 taagtttttc tgcctgtgaa ttttccagtg tcatattttg ccttcttacc tttattaaaa
     2221 tctaagatcg attaagcctc ttgaagcttt tttgtctcct gaaaccctat atgttgttcg
     2281 acagagtttt ttgtattcgc aagcggcaca ttgggtaaat ggaatgctct catcgagttt
     2341 gaatatattg tttgaaattt tttcaacata gtcttcgatg tgtttattta gcgcttgcat
     2401 tgtttcgtta aaatttactt cagctttgtc agtattgctt tcttcgtttt gaggatttcg
     2461 gtgcactata ttttttgcat aatacggttt tttatttttt ccttcattgt cttctatata
     2521 accgatgagc ggactaatcg ctggattatt aattgtaaaa aatgcagcac catcaactcg
     2581 cttgtttttt gttttttcaa aaagcctaat atataagggg agctgaaagt tttctagtct
     2641 tccttcttca tctgcaaggc aatcggtttt agatggaggg gagctagttt taaaatcgat
     2701 aatccatatt tcaccatttg gtttttcgac aaccaagtca atagagccaa gtagtaggta
     2761 tttttcttct tttgattcat gccaagcctc tgtttccaaa acacgtgagt tataaaagta
     2821 tgaaagaaaa attttaacag cctcatctaa tgttttgaaa aaagctggag cttgatttaa
     2881 aataatttct tggctcaaag gagaaaggtt ttcattggga aaaacagatt taggaaaacc
     2941 atccattatt gttttaaaag cggattctaa aacagggtag taagcttcgc ttgttttgtt
     3001 ttcttgaaaa gcgttttttg tttttggagg ttcagggagt gcttcgtctt tatctttata
     3061 atagataaaa aatgttttta gtatggcatg atagagttgt cctaagtata cgggagcaag
     3121 caaacttgtt tcgagtcgtt cgttttttaa tttatagtgc ctgctgaaaa gaaaaaagag
     3181 ggggcagaaa aaaaagtctc taagctttgt tgcagaaact ctcagcatat ttgtttctct
     3241 atcagaaaca cttgttaaaa atttttcata ggattcactg tcaagtgaaa atgtttttat
     3301 tttttcttct tgggggcgtg cttttatcca gtttgcaaaa ccatcttttt gtgttttgta
     3361 taaaaaatca gcttgagggt atgctgtttt tttatcggac tctgtttctc cttgtgtata
     3421 aaaaatattt tgctcaagaa gatagatatc ttttccctct attttttctg gtgctaagat
     3481 cttttgaaaa tctgtatggg ggagggaata atggctcagg cctttttcac tgtatgaaaa
     3541 tcgaacctgc cccatgctgt aaagtgcaag cattttttct gaaacattgc tgtcttctat
     3601 accgagtttt tgtcgttttg tcttgagtaa aaaagataag tattgatgta aaacagatac
     3661 ttgtttttgt cctgcgttta agataatgtg ttgccgatag ggagaggctg ctgcagcctt
     3721 gtagggaaag attgcgactc cttttgtttt ttgttgtgga agatattcgc ttgtatcgag
     3781 atgagagcaa aaaaatgcga gcgggttttt tacttgtacg ctaggatagc ttttttcaag
     3841 ctcgatacaa ttgagaagtt ctgttatgca acggcttagt ataaggtcgc tttcttcgct
     3901 acatatctcc atatcaaaaa acgtgctgcg aaattcaaag tagttttttt tcaatgcctc
     3961 aaaagacttt gcctttgcaa actctgtcgc tgagtttttt aaacttcgat aaaataaaac
     4021 taagctttca tcgtaatcga ttttaaaagc ctcttcccaa aggtctacca agactgtctt
     4081 ttttttataa gagcaaaggc aattgttttt taccccaaag ttaaggagct tatttataag
     4141 cgtttcaact gaagttttcc agggcaggtt tttattgaga aggatttgtt ttaaactgtc
     4201 aaaagagaac tcttcgtcta cgagtctttt catagaagaa aaaaagcttc ctgcgccata
     4261 gagcgagagt gctttcccag cccgcaactg aaagcctatg tctcgcagct ttagttcgcg
     4321 catgagatag ggggcgagtt cttccatctc tggaaggtga atcgctatct cgcttgctgc
     4381 atagcctgtg tcgagaagct cttcgatgaa gagggcggtt ttacgaatct cttcgcgtga
     4441 gttttcgtag gcgataacct gaaggttttc taaaacgctt tcttttgatg gagaagcgat
     4501 gagctttacg ttttctgcat tttccaaaat agatgcgtat tcagcatagt cgcttgtaat
     4561 gtctgcaaaa aaaacatagt aagacttacc atcgttatga aagggaggtt tttcccaagc
     4621 aggttcaaag agcccattgt tttcaagaaa ttgggcatag gtattttcaa gcttgagtaa
     4681 gtcgcggttt tctgcatcac cttctgtttt tgcgattttt tctagtattt ctttactttg
     4741 cttttcaaag ttgctgttct cgaagtttaa acttccttca cttagatttt gcactaaatg
     4801 cgctttgaag cgtgcaagtt gaggaagcaa agaggaaatc cagcttgaaa aagagggtgc
     4861 aaggtctgcc cattgttcag gtatgagggc tttgaaaaaa ggctcctccg tattttcaag
     4921 gaggttttct acgaaaagct tgcgtaaaag cgagggaata cttgctcgat cttgctttac
     4981 ggagcgaata gctgtccctt tgaaggtatc ccaggctata aatcgatcaa gttcgactgc
     5041 tttatagtct gttaaaaaaa gagatttttc agcccaggct ttcgctgtga tttctgtggg
     5101 gaaaacaaaa taggcatcct catttttgat agttttggag agaatgtcag ttattatttg
     5161 gtactcgctt tttttttctg taatttgatt tttcattatt cctattatat catagttttt
     5221 ggttagatac tgtttttttt agaaatgtat gttatacttt taaattatga aaatgtattc
     5281 agtgttacgg ggtaaggctt tacctcatgg agcaactata cacaaagatg gcgtaaattt
     5341 ctctgtgttt agccggcatg caacagcaat ttggctcgat ctttttgaaa aaccagaaga
     5401 tgatcttcct tttcagagca taaagtttga tgaaaagatt aataaaactg gtgacgtttg
     5461 gcacttgttt ataccaggat taaaagcacc tgcattttat ttatatagag tggatggtcc
     5521 ttttgaacct cataatgggc atcgctttaa caaaaacaac tatcttctcg atccctatgc
     5581 tcgagaactg actgatgtat ctgtttttaa acaattacac gatggctttt ttattcccaa
     5641 agatagttat gatatagaaa ggcaaaaacc acaaacagca aaaaaatttc ctaaatctgt
     5701 tgttgtagat gatgaatttg actggcaggg cgatacaccc ttaaatcttc ccttgcgtca
     5761 atctgttatt tacgaaacgc acttaaaagg ttttactgcc catgaaagtt ctcatgtaaa
     5821 gcatccggga acttataggg ggttgattga aaaaataccc tacttaaaag aactggggat
     5881 aacaagcgtt gaacttctgc ctgtacaaga atttgatgaa tgggaaaata caaatatcaa
     5941 tcccaaaact ggagaacggc tcaagaatta ttggggctac agcactattg ctttttttgc
     6001 acctaaaatt ggtttctcaa gtgataaaag tcctggtgga gcagtccgtg aatttaaaga
     6061 aatggttcga gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttattac
     6181 aagccacgat ggttttactt taaatgattt agtttcatat aacggaaagc ataatgaaga
     6241 aaatggtgaa aacaatcgcg acggctcgga ccacaatgta agctataact atggttttga
     6301 aggaccaaca caaaatcgtg acatagaaca aattcgaaat caacaaataa aaaactttct
     6361 ttgtagctta cttttttcgc aaggcacccc catgctgctt gccggcgatg aaatgcggcg
     6421 aacacaaaac ggtaataaca atgcctactg tcaagataat gccttaacct ggattgactg
     6481 gtctttaaaa gatacacact cgactctttt tcgctttacg aaacaggcta ttgcttttcg
     6541 cctaagccat cctgcctttc gccgtccaga gtttttccag ggacatgatc atagctataa
     6601 tgctatacct gatatttttt ggtatgatga gtttggagaa gctattgatt ggggcagtgc
     6661 aaatcatttt ctagcctttt atttggatgg gcgccatgca gaagtttttg cagatattga
     6721 tgataacgat ttttatgtaa tgctcaattc ttctacaaaa gataagacag ttgtagtgcc
     6781 cccactccaa aatgggcgaa agtggtatag agtaattgat acagcattac cgagtccgca
     6841 tgactttaca ccccctggag atgaagaaat tctgcaaaat caagcgaaat atgtgcaaaa
     6901 agcacgttct tttagtcttt tgttatctaa atagctagaa ttttttattc taatatccta
     6961 agattaggta taacacaaaa aaacagggcg taatgcctaa tatgggagca aaaaatgcaa
     7021 tttgacaaac acaaatttaa ggaaaatctt tctgcgcgtt tgcgaaggca atacgggaaa
     7081 gatattagcc aagcaagttt tcacaatttg tatgatgcgg tctctgcctc aactcttgag
     7141 tttattatgg aagattggat gcaaacaagg aaaacttttg aaactaaacc ggttaagcaa
     7201 atgtcctatt tatctgcaga gtttttaatg ggaagggcat tgaacaataa tctcattaat
     7261 acagggtttt tagaaccggt aaaagaagtt cttgaagaaa tgggaatcga ttatgcgaca
     7321 atagaggaac aagaatctga tgcgggtctt ggaaatggtg ggctcggacg tctcgcagct
     7381 tgttttttag attcgcttgc gacattagat tatccagggc atggctatgg cattcgctat
     7441 gagtatggta tgtttgaaca aaggattgaa aacggctttc aagtagagta tccagataat
     7501 tggttaaaac accgagatcc atgggaaata aaacgcagcg atttagcggt tgaagttgaa
     7561 tttggcggcc gagttgctat gaaaaaaggc tcgaatggct cggacgtttt ttatctcgaa
     7621 gatgcggaac gagttaccgc aacacctttc gacatgccca ttgttggcta tggaacaaaa
     7681 acagtaaaca cgctgcgact gtggcaggca acaagcccga atgggtttga cttacaactc
     7741 tttaatgaca tgcagtatca tcggtcggtt gaaaaacaaa atgatgcaga aaatattagc
     7801 cgtgttttat atccaaacga ttctggtcct tcagggaaaa cgcttcgcct gaagcaacag
     7861 tacttttttt catccgcgag tttacaagat ttactcaatg cctttgtaaa agaacacggg
     7921 aaaaattttg aactctttcc tgagtacaac gtgatccaac tcaacgacac gcatcctgtt
     7981 gtagctattc cagaaatgat gcgcttgctc atagatgtgt accgacttgg ttgggacgaa
     8041 gcctatagca tcgttgaaaa aacatttgcg tatacaaacc atactatttt atctgaagct
     8101 ttggaaaaat ggcctatagc aacttttaaa gaattgcttc cacgggtcta tcaaatcata
     8161 gaagaaatgc acagacgttt ttctatcaat ctcaaagaag cctttcctga agattatgct
     8221 cggcaaaacc gaatggcaat tattagcaac ggcatggttt ttatggcaca cttggctata
     8281 tcaacctgct ttagcgtaaa cggtgttgct gccttgcata cggaaatttt aaaaacaaaa
     8341 gagctgaaag actgggcaga tttttatcct caaaagttta ataataaaac aaatggcgta
     8401 acacaacgtc gttggctttt aagctcaaat ccacgacttg ctaaatttat tagcgcgcgg
     8461 ataggagacg gatggcaaaa agatttatct cttttgaaag ggctcgaaaa gtttgtagat
     8521 gacgatgcaa gtttaacaga acttatttct attaaacaaa agaacaaaga agaatttgct
     8581 gagtatataa aagttacaca aaatgaattt ttagatccgc agtctatttt tgatgtacaa
     8641 gtaaaacgtc ttcatgaata caaacgacaa ttgctcaaca ttttacacgt catgtatcta
     8701 tacaataaaa ttcttgaaga cgaaagctat aaacccttgc caagaacttt tatctttggt
     8761 gcaaaggctg catcagggta tcgaagagca aagacaatta tcaagttgat aacaacagtt
     8821 gcagaaagaa taaataatga caaacgagtg aaggggcttc ttcgtgttgt ttttttagaa
     8881 aactatagag taagtttagc agaaaaatta tttcccgcct ccgatgtgtc tgagcaaatt
     8941 tctactgcag gaaaagaagc aagtggaacg ggtaacatga agtttatgat gaatggtgcg
     9001 cttactattg gtactttgga tggtgcaaac attgaaattg tcgaagaagc aggtgatgag
     9061 aatgccttta tctttgggct taccgcagat gaaattcacg ccattgaaaa agagcatagt
     9121 taccaaccac aacgctatct tgactctaat ccagagcttg caaaagttgt agatcaactt
     9181 attgacggaa cttatgaccc tactaatcat cttttcaaag aacttcatga ttctttactc
     9241 tggggtgttg atgggcaacg aagtgatgtc tattatgtgc tcgcagattt tgaagcctat
     9301 gcaaaagcac atgaaaaggt tttagaagca taccaggatc aaaaaggctg ggcgaaaaaa
     9361 gccttgctca atattgcacg aagcggaaaa ttttcttcgg atagaactat agaagattat
     9421 gtctcggaca tttggaaaat agaaaaactc ttggtcgaat aaacatggaa caggttgggg
     9481 caaaaacact tcttgcatct ttgggcttaa tgcttgtaac tctgttgtgg ggactgtctt
     9541 ttgctgttgt aaaaaatagc ctcgacctcg ttcctcctgt gtatatgatg gcctttcgtt
     9601 ttagtatagc ctcgcttgca ctgcttctcg ttttcccaaa aaaactaagt gccataacaa
     9661 aggaaacact aaagaaaggt tttgttttag gcatttttct tgctctcggt tatgctttac
     9721 aaacgattgg agttgtgtac acaagcgcag gaaaaaatgc ctttataaca actacctatg
     9781 ttatctttgt accttttatt tattggtttt ttacaaaaca caagccaggt tttttttcta
     9841 ttatccccgc ctttgtcgga ctactcggca ttgcccttct ttccttacaa gatgatttaa
     9901 gtgtcaacaa gggcgacatt ctcacgcttt tatgcggtat ttcctatgcc tttcacatgg
     9961 tttttattgc gctttttaca aaagatgatg accccatact cttaaacctt gtgcagattt
    10021 tcttcgtaac agttttttct tggctccttg ctccctttta cgacggagcc tttcccttgc
    10081 ttgcaattca agaccccgca acgatttggt cgatgctctt cttggggatt ttttgtacga
    10141 tgcttgcttt tttcttacaa acgctttgcc aaaaatttgt gccaccaggg acagcctctc
    10201 ttataatgtc tctagaagcg gtatttggcg tcttttttgc ttggcttatc ttgggcgaaa
    10261 tcctatccct acgcatggct ttcggggccc ttcttgtatt tgtcgccatc ttgctttcac
    10321 aaaaaaaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg taaattgaca
    10441 gactaaaagg aaagctttaa tatagtagca tatatctgat aaaggaaaaa atcatgaaaa
    10501 aaaatctacg catttttgcg attattgcac ttgttgtact agcctttgca ttcactgcat
    10561 gcgctaagac tacaaagtct gacgggctta ctattaaaga gggcgttttg caaatcgcca
    10621 tggaaattgg ctatccgcca tttgagtatt tcgacacgga cgggaaaact cccatcggct
    10681 ttgatgtaca gctcggaaag gcaatcgccg aagaattggg cttgcaagtt gcctatgtgg
    10741 atacagcctg ggacggaatt ttcgctggtc taaaaatcaa taaatacgat gtggtcatat
    10801 cagcagtaac tataacagat gagcgcttaa aagaattcaa ttttaccaag ccatatatcg
    10861 gcaatgcgca atctctcgtt ttattgaaaa acacagagct gagtgcaaaa caccccgaag
    10921 aactcggtgg tttacgagtt gcctatcaag cagagaccac ttcagatatt tatatgacaa
    10981 aactaaaggc agaaaaaggt gtcaattaca aagagtttga atacgacaag gttatgaatt
    11041 gttttgacga tttgaaaaac ggacgagttg atgccattgt ttgcgatagc cttgtcgctg
    11101 ttgactatgt cgcggatgca aatgcgccct atgaaattgt ctggcttgga gaagcagatg
    11161 aattttttgg cgtgtgcatt aaaaaaggca atgatgctct tacagaaaaa atagacctcg
    11221 ccttagacaa actttttgca gaaggcaaga tacaagcgat ttcaaaagaa atttttggct
    11281 cagatattgt ttctgcgact cgcgcaaact aaataaaaac ctgtatttga aaaatacagg
    11341 cagaagggat actcatggaa aaactacaga caatacttaa ctggataccg ccacttttag
    11401 aaggcgcggt tgtaactatt tctctcactt tgctctctgt tatagcaggt ttaatcggta
    11461 gcttgttttt ggcacttgga aagatgtcaa aaaataaagc cctaaaccga gcttcgagtg
    11521 cctatgtttt ctttttccgt ggtaccccct tgttgatgca gttgtatttt atctattatg
    11581 ggcttccaca aattgctccc tggctcacta taaacaaccg atttattgcc gcctttatcg
    11641 cctttggctt aaactcagca gcctattgtg cagaaattat acgtgcggca attcaatcta
    11701 ttgacaaggg gcagtttgag gcagctcgtg ccttgggaat gagctatgga caaacgatgc
    11761 ggctcattat tattccgcag tcttttcgcc gtctcattcc acccgttggc aatgagttta
    11821 taatggtctt gaaagatgca tctttggttt cgattattgc cctatccgat ataacaaaaa
    11881 taacccgtag catttcttcg tcgacaggtt ctgcccttgt gtatattcct gcgatgattt
    11941 tatatcttat tattacgggg atttttaccc tcgttttcaa caaacttgaa aaaaaatact
    12001 cggtctacga ataaagcttc atactaaaaa taagaggaaa aactatggca atgataaaaa
    12061 caataaatgt atgtaaaaat tttggtagct tggaggttct ccgcgatgtt tctttgactg
    12121 ttgatcaaaa agaggtcgtt tgtatcgtcg gaccttctgg tgcaggaaaa tcaacctatt
    12181 tgcgaagttt aaatcggctt gaaaaaataa catcgggaca agtgtatatc gaagatgtgc
    12241 ttatgtttga tgccgaacat ggtgtaaata aggtgaaaat gcctgcaaaa gagagggcag
    12301 agcgtctttt ggaacttggt atggtttttc agcgctttaa tctttttcca cataaaactg
    12361 ttttagaaaa cgttatggtt tctcccatac atgtaaaaaa acaaagcctt tcgtatgcag
    12421 aagaaaaagc gatggccttg cttgagcggg ttggtttaag cgataagttg cacgaatatc
    12481 cggcaaaact ttcgggaggg caacaacagc gagttgctat tgctcgtgct ttggctatgg
    12541 agccaaaaat tatgctcttt gatgagccaa cgagctctct ggacccagaa ttggttgggg
    12601 aagtgcttgg ggtgatgaaa caactcgccc ttgacggcat gacaatgctt gtggttaccc
    12661 atgagcttgg ctttgcgcgc gaagtggcaa atcgtgtagt atttatgtat gaggggcgtg
    12721 tcctagaaga tgcatcgccc gatgagattt tcaaaaaccc aaaacacgaa agaacaaagc
    12781 aatttataaa aagcgtttta taaaataaag aggaggtcct tatggatatt tttacctata
    12841 cgctcattgt tgtttttttt atctttgcag cgatactttc ttttatagat ataaaacacc
    12901 aaaaaatccc ccgctttctt tctttttcgt ttattggcag tatctatggc ttacgtattc
    12961 ttggaaccct cctccttttt ttgatatcca atggcgagag tgagactggc aggctgtcat
    13021 tttccgcttt tttatttgcg ctttatccac cgacactggg acttatcttg ggactcgctg
    13081 tgttttacgc tgttcgtgtt gtatctaaac taaagctcgg actcgctgac gtgtggtttt
    13141 ctggagctat gggaactttc ttgggctacc ttggttggta tgccgccatc gcgatttcct
    13201 gtctttttgc gcttagctgg attttctccc ttgctgtttt acgaaagatt cgagcaaaac
    13261 atgaagggca aaaagaaata agcttgttaa aaacctttcg gcagattcaa atccctttta
    13321 taccctttct cgcatctggt tcactctgtg ctcttttagt ttcaagtctt acaagataat
    13381 atttttatct acctaagcgc tcgtattgaa aacacgaaaa gaacgtttgt tgagaatata
    13441 gagcacaaaa ccatagagga caatggtgaa gggaaggagc caggttccca aaaaacttgc
    13501 aataagcccg aatagaggcg gtaagattgt tgtcgaagta taggcgacag ccatttgaaa
    13561 gcccataatg gactgcgcgt gctcctttcc aaaacgtcga ggggtttcgt gtagcatgcc
    13621 aggaaagacg ggagcacaac cgagcccaac caagataaaa ccgatgagcg ctatttcgtg
    13681 cggcaagctt tgaatgagcg agagaagcag aaagagagag cccaagctta taagagatgt
    13741 gcctattttt aaaagttttt ttgatgaaag ccgcatcgcc aaaaaaccag agataaaacg
    13801 tcccgccata atgccggtgt aaaaaagact tgtccacaat gctgcgcgtt ctgcgctaaa
    13861 gaacaaagag tcttttaagt aacttgctcc ccacaagccc atcgttgctt caattcccgt
    13921 gtaaagcata aaagatatga ggacgccttt tactcccctt tgcgtgagga cttttttcaa
    13981 aggctctttt tgctttggct catccttgaa gatatctgca tctgggtgct cttttttttg
    14041 aagactttta tgattccaga gaggtagtgc aaaaaaaagg gcaagcgtca aggcaaactg
    14101 gataattgca acgaggaagt atgcagaatt ccacgaaccg tttttttgta aaaacaagct
    14161 cattataaca ggtccgctca aggccccaat cccccaaaaa ctgtgtaaaa aattcatgtg
    14221 gcttgcttcg taatgctccg caacgtactc gttcaaagca gagtctatag caccggcccc
    14281 aattccaagc ggtatcgcaa agagaaagag aggatagaga ctttgtgcaa atgaaaaaag
    14341 aaggagggcg agggcggtta aaaaaacaga taccagagat atcattcctg ttccgaattt
    14401 ttttattaac tttgcgctaa aaagcgatga gacaactgta ccgagtgaaa ttatcatgga
    14461 aataatgcct gcgcttccaa gaggaacagc aaattcaaga cgcattaccg gccaagcagc
    14521 accaagcata ccatcaggta aacccaaact aataaacgaa acgtagataa taattaataa
    14581 gagagtagcc atagtattga gtctactcta aagtaaaaaa gagtcaagct tgcaaaagcg
    14641 ttgctagtat ttttctactt gttcagataa aagcggtatc aattgttttt tacgtgatac
    14701 aatgtctttc aagacataca tatcttgttc ttgctttgga aaagttatgc tttgaataaa
    14761 gcttgcatct cccgatataa gcaagaaact cgtaagcttt gtaacatcgg taaccataag
    14821 agccgagaag agcaagtttt ttgctttttt cgcagtttca agtgcaaata aaaattcttc
    14881 ctttcgctcc attatctctt cggggttatc tacttcaatt tggcttaccg taaagctatg
    14941 ttctccttca gtgtattctt tcatatcttg ttgaataaca tcgttcgcgc tgcgttttcc
    15001 aatgcggctt gaagcactca taatatcgct accgagttcg tctatatcca agccggtaat
    15061 atttgaaaga tactcagcga catctcgatc tatgtcagtc gtagttgccg attgtaaaat
    15121 gagcgtatca gcgagaatcc ccgccaaaag gatggaggcg ataggtttcg gcatgggcac
    15181 cttgttttcc ataaaaagtg ttgcgataag cgttgatgtc gcaccaacag gcttgtttat
    15241 aaaggtaata gggtctcgcg tgttgagatt tcccagccga tgatggtcga taatttcctt
    15301 gattttataa tgctcaatgc cttcaatcgc ttgactgagt tcattgtggt cgaccattat
    15361 aacttcgata tttgcttctt gaatcaagtc gctttcagaa ataatcccga tgagtttttt
    15421 ttcatcgtta ataactggta aacaacgact aggcgaattt tgtaaaatgg gtttaatgct
    15481 acggaccaaa tcgtttttgt ttacaggttt tatgtccgtg tcagaaatag ccgatacggg
    15541 agtggagtag acaataagca ttgccgtact ggaagtgtcg taagggctaa taagcgcaca
    15601 tacatctttt ttttcagctt tcgcttgcaa gtcttttttc aaaactgatc cagctgtaag
    15661 aacaatcacc tttactcccc gttctatgca atattcttga atatcagctc tatcacctgt
    15721 aataacgatg agattttcag gtaaatgttc attgaatttt tcacaaaaag tatcgaactg
    15781 acttgcagct atgaggatgg cagagtttga tatgcggttt tcatcttttg tatgcaaggg
    15841 ctgtgcgcca agcgtatcgc aaataagctt aatactcgtt gtaactgacg ttttcttttc
    15901 agggtttagg atttttaaga cattttgagc aaaggcatta taatgcaaaa aagatttata
    15961 acgtccttct gaatctataa cagaaagcac ctttgagtta cattcttcca tttttgcaac
    16021 cgcattccaa agcgacgtgt tttcatgtac cgtttggtag gcttcattta tgtagaaacc
    16081 tacctttggg aggaggtcag aaaggtattc gggtacaggc actttaaagc gattgaaaat
    16141 gtattcgctt tgtgcagtca tttttccagc tcgtgcggca aaaaaatctt ttccaccgag
    16201 agattctttt aaaactgcat aactcgctgc cgaaaccaca gagtctgtgt ctgggtttcg
    16261 gtgcccgatt atatatgttt ttttattcaa tttctaagta caattctgtt tcaggaaggc
    16321 tgagagcgtt ggagaatgaa acttctttta catcgctttt attgccgagg ttatcgactg
    16381 cataaaatcg cacagtgttt tcgccttttt ctggtacaac gtaatcacct tgcacacttg
    16441 caaattcagc tccatttatg gaaacaaaga tatcttttac accagagcct tcatcgttag
    16501 cgacaaagct aagtactgtt gttgggtcga taaaaattct atcgttgagg acaatcgcat
    16561 ttgggtctat ttctatttct acttcaggtg ctgtattgtc aaggtatata agctgtgaaa
    16621 agatggggct tgtattccca acgcggtcga cgaggtaact ttccagttgg tatgatccgt
    16681 cttcgtaagt ttcatttagg taaatcatgt tttcatcgat aaaatctata tattcactat
    16741 cgtttatacg aacaaagagg ttttcggtac cacttccgat atcttctccc caaacgatga
    16801 ggcttgtatc gcttgtaaaa tagacgtttt gattttcatc gatataatac gcatcgtgga
    16861 taaggtatct taactcaggc gctgttccat caacgattgc tgtgtagtgt tttacggggc
    16921 taat
//
LOCUS       DAOSSW010000032        16609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_3574_length_16609_cov_6.823910, whole genome shotgun sequence.
ACCESSION   DAOSSW010000032 DAOSSW010000000
VERSION     DAOSSW010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 16609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16609
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3574_length_16609_cov_6.823910"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..2511
                     /locus_tag="PKW26_04205"
     CDS             301..2511
                     /locus_tag="PKW26_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOQ92803.1"
                     /translation="MSKIKKIAKKDFGFSFIPEAFLHPDQDEFTIRNRQINFNPSEWR
                     HISKEEILLLEKNNNTTDDWENVLVQDPFLPHLIKNSVFSGLVRIGRQEEVYLQYHDF
                     SLPVGITNSRIISCDIGENCAIHNCAYLSHYILGNSVILSCIDEMSTSSHAKFGEGVI
                     KEGESEDVRIWLSPINEAGGRLVLPFKEMICADAYLWAMYRDDAVLMERLKSITQQKI
                     DPRRGYYGQVGDETIIKSTRIIKDVNFGQACYVKGANKLKNLSIHSTRSESSQIGEGV
                     ELVNGIIGYGCRIFYGVKAVRFVLGNNSNLKYGARLIHSILGDNSTISCCEVLNNLVF
                     PGHEQHHNNSFLIATMIMGQSNMAAGATVGSNHNTRGNDGEIIAGRGFWPGLSSNLKH
                     NCRFASYVLLSKGNYPNELHIPFPFSLVIDNEKDNCLQIMPAYYWMYNMFALERNSWK
                     YKNRDTRKTIVQEVEYDYLAPDTVYEILEAIEKLEYLTGKAWFIREGMEPEFINNEDC
                     INKGKFLLENETAVVNSLSIVPEKIENSKRLVVLLRVAESYKAYKEMLVFYGVKTLAY
                     FLKESNLTWNQFSERQKKIDLHWLNLGGQLVPEKEVDALREKIREKKVNSWDEIHEQY
                     AIWHKDYPQKKAENAYQILQLLSMEEKIEEKNWESFLGEAIKIAKKVYEQVLFTKQKD
                     YNNYFRTITYRNEKEQNMVLGKIEKNPFILQAEKERDSFIHLCRDFLHKNEKIG"
     gene            2742..>4173
                     /locus_tag="PKW26_04210"
     CDS             2742..>4173
                     /locus_tag="PKW26_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOQ92804.1"
                     /translation="MLIATTFAVIILLLRSIIKSVTNLTDKTSYIKQKELSIQFLENS
                     KDDLSEFTKALNEFLLFYNDFFRHLKEMCLENQSAHLSVVDLNKKIIDVIKGARKSVE
                     EVSDKMYSLNSNIASADELSNSISIDAVTLSSQIEIQVTMVQNSIEALEAITKNIEIL
                     YSSAEKTTDNVTHLVSLSTNGKNTMDISNEKILEIETQAFKINEVVDLIEQIADQTNI
                     LAMNAAIEAAHAGDAGKGFSVVAEEVRKLAEASAEGSKDISTFVSGIIGQIKNTKEAS
                     LATGEAFNDIDRTIHQVNKEVSIISESLNDIAQKSSGVMNNILMLKDISEMIDLSSKE
                     VATRSERISEDMKVVDGISSNVFEKNTDLLSHIHTLEESSRSFEEAIGKLQGVSQTLN
                     EKLYSIQINDVCATIDFETEFESEGKNITLEQSESLEIQNTEKDEVVQISIDEKEKSE
                     SQFMEENSFLEEIVQDSSVQESSTKEI"
     assembly_gap    4174..4273
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4448..6211
                     /gene="mnmG"
                     /locus_tag="PKW26_04215"
     CDS             4448..6211
                     /gene="mnmG"
                     /locus_tag="PKW26_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOQ92805.1"
                     /translation="MKKSIESYDVAIVGGGHAGIEAALAASRMNLKTILITQSISNIG
                     VLSCNPSIGGISKGNIVREIDALGGEMGKIIDKAMIQFRLLNKSRGPAVQAPRSQADK
                     NYYASIARQSLETQENLSLLQDTIVDIQVEEKEKTYLKALLTERGRLIPCRAAVLTTG
                     TFMGGKIFIGQFDAPNGRLGELGAYGLTESLQKLGFKIGRLKTGTPQRVLKCSIDFSK
                     FEIQNGDETIIPFSFDNEKIERPMVPCYLGYTNEKTHEIIRENILKSPLYAGKISGVG
                     ARYCPSLEDKVMRFAERDKHQIFIEPESLDSNEMYINGVSSSLPEDVQDAFLRTIPGF
                     EHAKVTRPGYAVEYDFIEPTQLYPSLETKKVVGLFSAGQINGTSGYEEAAGQGLVAGV
                     NAALRVKAMQKNQVEYEPLFLARDEAYIGVMIDDLVTLGTKEPYRMFTARAEYRMNLR
                     HDTANERLCEKGFKVGLKTKAQLDAVEEKKALKKEILSLLKTKRGVQNPGYEKRIWEE
                     AEIDYKYEHYILKQEKRVEKLKKMEHFSIAADFDYSKIVGLSNESRQKLEEIRPTSLG
                     QAGRISGIRSSDIMLLMIYLK"
     gene            complement(6203..7114)
                     /locus_tag="PKW26_04220"
     CDS             complement(6203..7114)
                     /locus_tag="PKW26_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOQ92806.1"
                     /translation="MISFSEFLDIPKIDAHNHLNLGMRYSSYAPWAGFYIPNFPRKMS
                     GLDEMHEIIGEYTRPRVQSAKDVSTLFSLSIEDAIEDGIKILEASVDMGLAQFFRNTT
                     AFCNMLESLKSQYIKEISFRPEVGMGKTFDKAKLKRLVPDCIQSGVFGSIDLYGPEVL
                     DGIEDFQAYFELAYKKGLKKKAHIGEFSNAQTVKDFVNFFELDEVQHGINAAYDDSAL
                     LLLKEKNVRLNITPTSNVMLSAVMSLESHPIKRFVQEGVRVSIATDDLLFFNRSVSEQ
                     CADMVNAGLFTKEEVLDILNQSYEESL"
     gene            complement(7137..8942)
                     /gene="pepF"
                     /locus_tag="PKW26_04225"
     CDS             complement(7137..8942)
                     /gene="pepF"
                     /locus_tag="PKW26_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOQ92807.1"
                     /translation="MSTKSIPLRKDVKQEDKWDLTSLYNSLEEWETDLKTVPEMTKTL
                     ASFKGRLAENEENLLEALKAFSKLEEAIEKLASYAFLLTTTDASDSSYQDIYGRIMIA
                     ASKSEEELSFFVPEIQSIPEEKLTKWIETDNFADFKVAIEKLLWLKPYILSEKEERLF
                     ALQMEANGTAQKAFSMLTNVDLDFGTIKTDAGEEALSQSTYSIFLESANRDIREAAYK
                     KFYGTFDNHKNTLASLYAGSVHLDIYNARARGYTSSIEAALYRDKVPLSVYENLIATI
                     HEHKAVFHKYYSLRKKILKLDTLRHWDVYVPLVPEVSRITPYAEAVDILREALRPLGN
                     EYTDILCSGLLNGWVDKYENKGKRSGAFSAGGYTGYPYILMNYKENVLRDVFTLAHEG
                     GHSMHSYYSARNNPFLSYNYTIFEAEVASTFNEDLLFHYLLKHSADNTMKAYLLCNRA
                     SDILATLYRQTMFAEFEKITHEKQESGQPLTLDLLRSEYRNLLVSYFGPEMQFESESD
                     LESLRIPHFYSAFYVYKYATGVSASLALAKKVREGTEQERQDYFKFLSSGGSAYPIQA
                     LKKAGVNMEEKGPIRAAIQEFENLVTEMEKLLLVQ"
     gene            9053..9976
                     /gene="pyrB"
                     /locus_tag="PKW26_04230"
     CDS             9053..9976
                     /gene="pyrB"
                     /locus_tag="PKW26_04230"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOQ92808.1"
                     /translation="MLKGKHVIEPSNLSVKEIDEICSLAEQILVDPAAFMDVCRGKIL
                     ATLFFEPSTRTRLSFEAAIMRLGGTFLGFADALSSSTAKGESLADTIRTVAAYSDIIA
                     MRSPKEGAAMLASKYSSKPIINAGDGGHQHPTQTLTDLLTIRSIKGGFSNLTIGLCGD
                     LKFGRTVHSLIKAMSRYSNVKFVLISPTELQIPSYLESQILKPLGIAYTSVEQLDDVI
                     GDLDILYMTRVQKERFFNEQDYIRLKDSYILDAEKMKGARKNLIVMHPLPRVNEISPE
                     VDSDPRAVYFKQVEFGMYARMALIAKLTGAI"
     gene            9989..10429
                     /locus_tag="PKW26_04235"
     CDS             9989..10429
                     /locus_tag="PKW26_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOQ92809.1"
                     /translation="MIHVDTIKNGLVIDHIRAGSGPRIFSWLGLDKADFSVAMIMNVP
                     SKTHGKKDIIKIDSIISIDYSVLGFIDPNITINVVVEERITRKINLTLPDRVENIIAC
                     KNPRCITSTEKYITHVFTLVDKKRAEYCCEYCDELYRAGIGEVF"
     gene            10429..11694
                     /locus_tag="PKW26_04240"
     CDS             10429..11694
                     /locus_tag="PKW26_04240"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOQ92810.1"
                     /translation="MKKIIYNVRIVDTNTDEYGAVVIRKGKIESVVLLGKDVSNICVA
                     PSLRKLIDSCDEKINAKGLVLMPAFVDWHAHFRYPGQGEKEDLETGLAAARAGGFSTV
                     VLMPNTNPVVSSQKEAVSIMDEIKLLSDIKAFQTVSITKNFDGRDISHLHALDKSIVP
                     IITEDGFDVASESVMREAMKIAAKKGFVVSCHCEDAAFVPLAKKAREEGNFKKAEKLL
                     QKAEDTATKRNLKLALETGCRVHIAHCSTKKSLAYVRQAKKKETFPGQITLEVTPHHA
                     CLSYEMKGLDKELVNPPLRSEKDRRAVVKAIQKGLVDVIATDHAPHTLEDKKNGACGF
                     SGIETAFSLCYTELVKTGKLSLSKLSELMSSNPAQMLGLDKGFITEGLDANLVLLDLE
                     ASFFIDSKDFKSKGKYTPIQGKKLFGRII"
     gene            11740..13356
                     /locus_tag="PKW26_04245"
     CDS             11740..13356
                     /locus_tag="PKW26_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ92811.1"
                     /translation="MYKTLIFRSLSRHKKKGRRLFVLMALCSATIILLLSFKEDFKRQ
                     SEEQLVQLRYGHLEIVPPDSPLAQKNLITPPTENLSFINFDKDFDIWLRSLPEVAEAS
                     PSITRYGIAYSLGGETESWLALLAVDAKRLASLLPLTKALEGSDDISWTEGEDVPILR
                     ATPYSIFGSYTENPDIFYRYQLRANDEGLPQFFSEISKDFPKIFISPPYQNAADDWNR
                     FLTEWNAFLIKEDVHEWLDEIPERFFEDYDWKLDDAIWELRENTNFKRKAFLKKKVFQ
                     ALYPNGIVELREPIYVDKQVTLQLPLINAKEVIALPVAVNAMYKGFCAVDPLYQEVSF
                     MDINAFRYYMGLKENEATEYIVRLHKSSDVEKIKAVIEDKLLEMGSDAKVVDWKSLGE
                     MQMTTATAFTFVIGLLIGIFIIIVLIFTVNLVLISLLQRKKEIGTGLAMGLSTDQTRL
                     LMSAEVGLIAFVSCFLGSVFALVLIAFFSKIGIPGMVFFVSGKLFLQFRFYPFIMAFF
                     ILVPSTMLVSLIPLSQLHEKMPVDFFKEGK"
     gene            13356..14618
                     /locus_tag="PKW26_04250"
     CDS             13356..14618
                     /locus_tag="PKW26_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92812.1"
                     /translation="MKLALQIAFRNISSHKRKNRIIFAVTTVLSFFMFLFLAFSDGEI
                     ENIKNGVSSFYRPHWDLRVFTKEFIRLDHRGENPWEETILNADELKKDLLAFPEIKEV
                     ISYLPANNVSLFINGKKYLYFSPIAVEKNDSFIQSKYEIVEGNREVIPEKNTISLHYM
                     MKKLIGAKIGDEIKIVGDTIFDQVTMAKVRVSSFYKPYFDNPNLASSFLMPFEDLQNY
                     VGYEQNEAPYLIIRLQAKVNPEKMQKQLISWAEDTNKELRFFTQKTSSRQDQWAMIYG
                     MIRYIIIAMTLITFFIAAFGMMNVVSVNLLERAKEIGTYYCLGCEKPFLMAVYTIEIF
                     LVNMIGGIAGILLGQGARLIVNALKITSEEPGFQIVVGSSVFYLGSSLSTVVWIIGGL
                     AIITILTSLSTLGKALKVSPVVAVQELN"
     gene            14635..15375
                     /locus_tag="PKW26_04255"
     CDS             14635..15375
                     /locus_tag="PKW26_04255"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOQ92813.1"
                     /translation="MKNKIFKTISINLCILSVSFAFLYGQSAEEIIKKMEEKMFPSSR
                     SEMTLRFETKTSQEDYVFTSFARDTNQKIIVRFKSPAAMLGSDLLMLDRNVWLYDPKS
                     GREMKIPSNQSFGGTGFSYGDILRLNYSDNYSASIKEDLSDSWIIDLVAKQKDAPYHA
                     ISLEIAKDFTPVLGRYFTRSGDLIKEMEYSNAKLINGVLKPLTVRVTSPLDPSEVSTL
                     SLVKEEKKEYPLSIFNKRNLSLKMEENY"
     gene            15391..16062
                     /locus_tag="PKW26_04260"
     CDS             15391..16062
                     /locus_tag="PKW26_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ92814.1"
                     /translation="MTFIETKDLIKNYELGKTLVPALRGLDVAIEKGEYLCVAGPSGA
                     GKSTFLNLVGLLDEATSGTIIFDGTDITSLSKKNYHRFRRGKISFIFQSFNLVPVLNA
                     RENIEFPLIIQKIDKAERDRRIDAITEEIGIRQWLHHKPDELSGGQRQRVAIARALVT
                     KPEVIIADEPTANLDSVTGKAILDLLRRLNSEEQTTFIIASHDHSLIDGADRVIKIVD
                     GLVAS"
     gene            16059..>16609
                     /locus_tag="PKW26_04265"
     CDS             16059..>16609
                     /locus_tag="PKW26_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92815.1"
                     /translation="MKSFVVRLHYKKSFFLLLLFSFAFTILAAQETDAEKKLETKLET
                     GFAFTAYEPSIDSTWFTKESLYLDFTLTSGNILKANGFLEAVYDDENKAPEPLTAEEL
                     IKELSLSWMISPSFIATVGKQKLEWGVARAFPSIDSLEALTSVSEPSEKAIPRGLTGF
                     KLDLIPSWFWSFSLLAVPEPRLR"
BASE COUNT         5288 a         2761 c         3410 g         5050 t
ORIGIN      
        1 tctcaaaaac tcggtaagta aacggaacgt ttacgccaga tgccaggctt tttttattta
       61 aaagcggtct tgcgccgctt ttaaataaaa ttagtctaag gttttctatc gcgagttttc
      121 tcaagctcct tcaaagccct cactttccct ctcaaaaact cggtaagtaa acggaacgtt
      181 tacgacagat gccaggcttt cggcctaagg ttttctatcg cgagttttct caagctcctt
      241 caaagtcctc gctttccctc tcttgaacac aggtgccgtt ttcctttata ctaaaagcct
      301 atgagtaaaa taaaaaaaat agcaaaaaaa gactttggtt tttcttttat tcctgaggct
      361 tttttacatc ctgatcaaga cgaatttact atccgaaata gacaaataaa ttttaatcct
      421 tcagaatgga ggcatatatc caaagaagaa attcttttgt tagagaaaaa caataacacg
      481 acagatgatt gggaaaatgt tctagtacaa gaccccttcc ttccgcactt aataaaaaac
      541 tctgtctttt caggcttggt aagaattgga cgtcaagaag aagtgtattt gcagtaccac
      601 gacttcagtt tacctgttgg tataacaaat agcagaatta tttcttgtga tattggagaa
      661 aactgcgcca ttcataattg cgcttatctt tctcattata ttttgggaaa ttcggttatt
      721 ctgagttgta ttgatgaaat gtctacttca agtcatgcaa agttcgggga aggggttata
      781 aaagaaggag agagcgaaga tgtccgtatt tggctttcac ctataaatga agctggtggg
      841 cgccttgtac ttccttttaa agaaatgatt tgtgcagatg cctatttatg ggcaatgtat
      901 agggacgatg ctgttttaat ggagcgttta aaaagtataa ctcagcaaaa aattgatccg
      961 cgtcggggtt attatggaca agtaggtgat gaaactatta taaaaagtac cagaattata
     1021 aaagatgtta attttggaca ggcatgttat gtaaaaggtg cgaacaagct taaaaaccta
     1081 agcattcatt ctactcgttc agaaagttcg caaattggtg aaggcgtaga gctcgtaaac
     1141 ggtattattg ggtatggctg tcgtattttt tatggtgtaa aagcagttcg ctttgtttta
     1201 ggaaataatt ctaatcttaa atatggagcg cgtcttattc actcaattct gggagataat
     1261 tccaccatat cttgctgcga ggttttaaac aatcttgttt ttcccggtca tgaacaacat
     1321 cataacaatt cttttttaat tgcaaccatg ataatggggc aatcaaatat ggcagcgggt
     1381 gcaactgttg gttcgaatca taatacgagg ggaaatgacg gtgagataat tgctggtcgt
     1441 ggtttttggc cagggctttc cagtaactta aaacataact gtcgatttgc aagttatgtt
     1501 ttgttgagta aaggaaatta tccaaatgaa ctacacatac cctttccgtt ttcacttgta
     1561 atcgataatg aaaaagataa ttgcctgcaa ataatgcctg catactattg gatgtataat
     1621 atgtttgcac ttgaacgtaa ttcatggaaa tataaaaatc gagatactcg aaaaaccatt
     1681 gtccaggaag ttgagtatga ctatctcgca ccggataccg tttatgaaat cttagaggct
     1741 attgaaaagc ttgaatatct aacgggtaag gcttggttta tacgagaagg tatggaaccc
     1801 gaatttatta acaatgaaga ttgtataaat aagggaaagt ttctgctcga aaacgaaacg
     1861 gctgtggtta attctcttag catcgtacca gagaaaatag aaaactcaaa acgtctcgtt
     1921 gtcttgcttc gcgttgcaga atcgtataag gcctacaaag aaatgcttgt gttttatgga
     1981 gtaaagacac ttgcttattt tttgaaagaa tctaatctta cttggaatca attttcagaa
     2041 agacaaaaaa aaatagattt acattggctt aatcttggtg gacaattggt gcctgaaaaa
     2101 gaggtagatg ccttacgaga gaaaattcgt gaaaagaaag taaactcttg ggacgaaatc
     2161 catgaacaat atgcaatctg gcacaaggac tatcctcaaa aaaaggccga aaatgcgtat
     2221 cagattttac aattactgag tatggaagaa aaaatagaag aaaaaaactg ggagtctttt
     2281 ttaggagaag ctataaaaat agctaagaag gtatacgaac aggttctctt tacaaagcaa
     2341 aaagattata ataattattt tagaacaatt acatatcgaa atgaaaaaga acagaatatg
     2401 gttttgggta aaattgaaaa aaaccctttt atactgcaag ccgaaaaaga aagagattct
     2461 tttatacatc tttgcaggga ttttttacac aaaaatgaaa aaatagggta gaataggctt
     2521 gtaagtgccg acatgaggag taggttttca acatcgttta ggggaagttt tggaaaatct
     2581 ttttctggag gtatgatgac aattagaaaa aaataattac cttaggtgtt ctcattgtac
     2641 ttttatccgt ttcggtagcg tttttttctc tcagtttaca aaaatccttc ataacaaata
     2701 aggtatatgc gaattcattt gccgtaaata gtattcttgg tttgcttata gctacgacct
     2761 ttgctgttat tatattgtta ctgcgttcaa taattaaatc ggtaacaaat ttaacagata
     2821 aaacgagtta tattaagcaa aaagaactaa gcattcaatt cttagaaaat tcaaaggacg
     2881 atttatctga atttacaaaa gctttaaacg aatttttact tttttataat gattttttta
     2941 ggcacttaaa agaaatgtgt ttagaaaacc aatctgctca tctttctgta gttgatttaa
     3001 ataagaaaat tattgatgtg ataaaaggtg cacgaaagtc cgtggaagaa gtgagcgaca
     3061 agatgtattc tttgaattca aatattgcaa gtgcagatga actttcaaat tctatttcta
     3121 tcgatgcggt tacattatcg agccaaattg aaatacaagt tacgatggtg caaaattcta
     3181 ttgaagcttt agaagcaata acaaaaaata ttgaaatatt atattcttcg gctgaaaaaa
     3241 caactgataa cgtgactcat ttagtttcgc tttctaccaa tggtaaaaac accatggata
     3301 tttcgaatga gaaaatttta gaaattgaaa cccaagcttt taaaataaat gaagtagttg
     3361 atttaatcga acaaatagca gatcaaacaa acattctcgc aatgaatgct gcaattgaag
     3421 cagcccatgc cggagacgcc ggtaagggct tttctgttgt tgctgaagaa gttcgtaaat
     3481 tggcagaagc atcggcggag ggttcaaaag atatttctac ctttgttagt ggtattatcg
     3541 gtcaaataaa aaacacaaaa gaggcaagcc tagcaacggg cgaggccttt aatgatatag
     3601 atcgcactat acatcaggta aacaaagagg tttctataat aagtgaatct cttaatgata
     3661 ttgcgcaaaa aagcagtggc gtaatgaaca atattctgat gcttaaagat atttctgaaa
     3721 tgatagattt aagttcaaaa gaagtagcca ctcgctcaga gagaatttct gaagatatga
     3781 aggtggttga cggcataagt agtaatgtat ttgaaaaaaa cacagactta ttgtcacata
     3841 ttcacacctt ggaggaatct tcaagatctt tcgaggaagc aattggaaag ttgcaaggtg
     3901 taagtcaaac tttaaatgaa aagttatata gtattcaaat aaatgacgtt tgtgctacga
     3961 tagattttga gacagagttt gaatcagagg gtaaaaatat taccctagag caaagtgaaa
     4021 gtctagaaat acaaaatact gaaaaagatg aagttgtgca gatctcaata gatgaaaaag
     4081 aaaagagcga aagtcagttt atggaagaga attccttctt agaagagatt gtgcaagatt
     4141 cctcggttca agaatcgagt acaaaagaaa tagnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnttagaag agattgtgca agattcctcg gttcaagaat cgagtacaaa
     4321 agaaatagaa aaaatagaac aagaaaatga gaagcaaaca aaagaagaaa ctgttgatat
     4381 tgatttagat gattttttta attaagccta tcttaacaaa aacgagagaa aaaactatac
     4441 tatacaaatg aaaaaatcaa ttgaatccta tgatgttgcg attgttggcg gagggcatgc
     4501 tggaatagaa gctgctctcg ctgcctcccg aatgaactta aaaacaattc ttataacgca
     4561 atccatttcg aacataggag ttttatcctg taacccgtcg attggaggta tctccaaggg
     4621 aaatattgta agggagatag atgccctcgg cggtgagatg ggaaaaatta ttgacaaggc
     4681 aatgatacag tttcgccttt taaacaaaag tcgaggacct gctgtacaag ctccgcgctc
     4741 tcaagctgat aaaaactact atgcttctat tgcacgacaa agtctagaga cgcaagaaaa
     4801 cttgagtctt ttgcaagaca caatcgtaga tatccaagtc gaagaaaaag aaaagacata
     4861 cttaaaagcg cttcttaccg agcgtggtcg gctcattcct tgccgggctg ctgttcttac
     4921 tacgggcacc tttatgggtg gaaaaatctt tatcgggcag tttgatgcgc caaatggcag
     4981 gctcggggaa ctcggcgcct atggcttgac tgaatcgctt caaaaactcg gttttaaaat
     5041 agggcgatta aaaacaggta ctccgcaacg tgttcttaaa tgctctatag atttttccaa
     5101 gtttgagata caaaatgggg atgaaacgat tattcctttt tcatttgata atgaaaaaat
     5161 cgaaagaccg atggtgcctt gttatcttgg ctatacaaat gaaaaaaccc atgaaattat
     5221 tcgagaaaat attttaaaat ctcccttata tgcaggaaaa atttctggcg tcggcgctcg
     5281 atattgccca tctctcgaag acaaggtgat gcgctttgca gaacgagata agcatcagat
     5341 ttttattgaa ccagaaagtt tggattcaaa tgaaatgtat ataaacggtg tgagttcttc
     5401 tttgcctgaa gatgtgcaag atgctttttt gagaacgatt cctggttttg agcatgctaa
     5461 ggttactcgc cctggctatg ctgtcgaata cgattttatt gaaccaacac aactctatcc
     5521 gtctctggaa acaaaaaaag tggtgggtct tttttctgcc ggtcaaataa atgggacttc
     5581 aggctatgaa gaagctgcag gccaaggttt ggttgctggg gtgaatgcag ctttgcgcgt
     5641 aaaggctatg caaaaaaatc aggtagaata tgagccactt tttttggctc gggatgaggc
     5701 ctatatcggc gttatgattg atgacttggt aacgctcggt acaaaagagc cctatcgtat
     5761 gtttaccgca cgggctgagt accgtatgaa tttgcgccat gataccgcaa acgagcgact
     5821 ttgtgaaaaa ggctttaaag tgggcttaaa aacaaaggca cagctcgacg cagtggaaga
     5881 gaaaaaagcc ttaaaaaaag aaattctttc cttgcttaaa acaaagcggg gtgtgcaaaa
     5941 tcctggttat gagaaaagga tatgggaaga agctgaaata gattataaat acgagcacta
     6001 tatcctcaag caagaaaaac gagttgagaa gctaaaaaaa atggagcact tttccatagc
     6061 tgctgatttt gattatagca agatagtcgg cctttcaaat gagtcgcggc aaaaacttga
     6121 agaaattcgc ccgacaagct tggggcaagc agggcgtatt tcgggaattc ggtcatcaga
     6181 tataatgctt ttgatgattt acctaaagtg attcttcgta gctttggttt agaatgtcta
     6241 aaacttcttc cttggtgaat aagcccgcat tgaccatatc ggcacattgt tcactaactg
     6301 aacgattaaa aaagagtaag tcatcggtag cgatggaaac tcgtactcct tcttgtacaa
     6361 aacgttttat ggggtggctt tccaaagaca ttaccgcaga aagcattacg ttacttgtgg
     6421 gagtaatatt gaggcggaca tttttttctt tcaataacag taaggcagag tcgtcgtagg
     6481 ctgcatttat accgtgttgt acctcgtcga gttcaaagaa gttgacaaaa tcttttaccg
     6541 tttgtgcatt tgaaaattct ccaatatgag cttttttctt taatcctttt ttataggcaa
     6601 gttcaaaata ggcttgaaaa tcttctatgc catctaaaac ttctggtcca tagagatcaa
     6661 tacttccaaa aacgccgctt tgtatacagt ctggtacaag cctttttagt tttgccttat
     6721 caaaggtttt ccccatgcct acttcgggac gaaagctgat ttcttttata tattggctct
     6781 ttagggattc aagcatattg caaaaagcag tcgtatttct aaaaaattgt gcaagtccca
     6841 tatcaacact tgcttcaagg attttaatac cgtcttctat cgcatcttct atagataagc
     6901 taaaaagcgt gcttacgtct tttgctgatt ggactcttgg acgtgtatat tctcctataa
     6961 tttcatgcat ctcatctaag ccagacattt ttcgaggaaa atttgggatg taaaaacctg
     7021 cccatggggc ataggatgaa tagcgcattc ccaagtttag atgattgtgt gcatcgattt
     7081 tgggaatatc taaaaattct gaaaaagaaa tcattacgtc aactccgttc atgtttttat
     7141 tgcactagta gcaatttttc catttctgta acaagatttt caaattcttg tatcgcagct
     7201 cgtataggcc ctttttcttc catatttact cctgcttttt ttaaagcttg aataggatag
     7261 gctgaacccc cgctcgataa aaacttaaaa taatcttgtc tttcttgttc tgtaccttct
     7321 cttacttttt tagctagggc taaggaagca gagactccag ttgcgtattt atagacgtag
     7381 aaagcagaat aaaagtgagg gatccgaagt gattcgagat cgctttcgct ttcaaattgc
     7441 atttctggtc caaaatagct tacaagtaag tttctatatt cacttcgtaa taaatcaagc
     7501 gtcaaaggtt gtccgctttc ttgtttttcg tgtgttatct tttcaaactc agcaaacatt
     7561 gtttgtctgt agagtgttgc caaaatatca gatgcgcgat tacaaagtaa atatgccttc
     7621 atagtattat cggcagaatg tttcaaaaga taatgaaaaa gtaagtcttc gttgaaagtt
     7681 gatgcaactt cagcttcaaa aatagtataa ttatagctta aaaaggggtt atttcttgca
     7741 gaatagtagg agtgcataga atgtccgcct tcgtgtgcga gggtgaatac atcgcgtagg
     7801 acattttctt tgtaattcat caagatatag ggatagccgg tatagcctcc tgccgaaaat
     7861 gcaccgcttc gctttccttt attttcgtat ttatcaaccc agccattcag taatccagag
     7921 caaagaatat cagtgtattc gttgccaagt ggacggagag cttcccgtaa gatatcgaca
     7981 gcttcggcat agggagttat tcgagaaact tcgggtacga gggggacgta gacatcccaa
     8041 tgtcgtaagg tatcgagctt gaggattttt tttcgtaggc tatagtattt gtggaaaaca
     8101 gccttgtgtt catggatggt ggcgatgagg ttttcataga ctgagagggg taccttatca
     8161 cggtagagtg cagcttctat gctcgatgta tatccgcgag ctctggcatt gtaaatgtcg
     8221 agatggacgc ttcctgcgta gaggcttgca agggtgtttt tatgattgtc aaaggtgccg
     8281 tagaattttt tataggcagc ttcgcgaata tctcgatttg cgctttctaa aaaaatggaa
     8341 taagtggatt ggctgagggc ttcttcccct gcatcagttt ttattgtgcc aaaatctaaa
     8401 tcaacatttg tgagcataga aaaagctttt tgtgcggttc cattcgcttc catttgaagg
     8461 gcaaagagtc gttcttcttt ttcagataag atatagggct tgagccacaa gagtttttct
     8521 atagctactt taaaatctgc aaagttatct gtttctatcc attttgtaag tttttcttcc
     8581 ggaatgcttt gtatttctgg tacaaaaaaa ctcaattctt cttcggattt acttgcagct
     8641 atcattatcc tgccgtagat atcttgatag cttgaatcag aggcatctgt agtggttaat
     8701 aaaaaagcgt aggatgcaag tttttctatt gcttcttcaa gtttgctaaa agctttaaga
     8761 gcttctagta aattttcttc gttttcagcg agtcggcctt tgaaagacgc gagagttttg
     8821 gtcatttcgg gtacagtttt gagatctgtt tcccattcct caaggctatt gtaaagactt
     8881 gttaggtccc atttgtcttc ttgttttacg tctttacgta aaggtataga ttttgtactc
     8941 ataataataa agtataacaa gctttagatg aaataacaag gaagtttaat tttttttcta
     9001 gcttgagaaa aagaattaaa gtatgatata ctgtgggtag aaaaggggca gtatgttaaa
     9061 agggaagcat gttattgagc cgagtaattt aagtgtaaaa gaaatagatg aaatttgttc
     9121 tttagcggag cagattcttg tagacccagc agcatttatg gatgtgtgta ggggcaagat
     9181 tcttgcgacg cttttttttg agccaagcac gagaacgagg ctttcttttg aagctgcgat
     9241 aatgcgtttg ggtggtacct ttttaggttt tgcggatgcg ctttcaagct cgacagcgaa
     9301 gggggaaagc ttagccgata ctattcgaac tgttgctgcc tatagcgata ttatagcgat
     9361 gcgaagtccg aaagaagggg ctgcaatgct tgcatcaaaa tattcttcaa aaccaatcat
     9421 aaatgcaggg gacggtgggc atcagcatcc gacacaaacg cttacggatt tgctgacaat
     9481 tcgttctatt aagggtgggt tttcaaacct tacgattggt ctttgtggtg accttaagtt
     9541 tgggcgaact gtgcatagtc ttataaaagc gatgagtcgg tattcaaacg tgaaatttgt
     9601 tctcataagc ccgacggaat tgcagattcc tagttatctt gagtcgcaga ttttgaaacc
     9661 tctaggtata gcctatacaa gcgtcgagca actcgatgat gttattggag acctagacat
     9721 cttatatatg acgcgtgtcc aaaaggagag attttttaac gagcaagatt atattcgcct
     9781 aaaagatagt tatattttgg atgcagaaaa aatgaaagga gcacgaaaaa acctcattgt
     9841 tatgcacccg cttccacgcg tgaatgaaat ttctcctgaa gttgactccg atccgagggc
     9901 ggtttatttt aagcaggttg agtttggcat gtatgcgcgt atggcgctga ttgcaaaact
     9961 caccggtgca atctaaagga agaatagaat gatacatgtt gatacgataa aaaatggttt
    10021 ggtaatcgac catattcgcg caggaagcgg gccacgcatt ttttcatggc ttggtctcga
    10081 taaggccgat ttttcagttg cgatgattat gaatgtgcct tcaaaaacac atggaaaaaa
    10141 agatattata aaaatcgata gtattatttc tatagactat tcggtgcttg gttttattga
    10201 cccaaatata acgataaatg tcgtggtgga agagcgcatt acgagaaaaa taaacctcac
    10261 tctgccggac cgtgttgaaa atattattgc ttgtaaaaat cctcgttgta taacaagtac
    10321 agaaaaatat ataacccatg tttttactct tgttgataaa aaacgagctg agtattgttg
    10381 tgaatattgc gatgagcttt atcgcgcagg gataggtgag gttttttgat gaaaaaaatt
    10441 atatataatg taagaattgt tgatacaaac acagacgagt atggggctgt tgtgatacga
    10501 aagggaaaaa ttgagtctgt ggttttattg ggtaaggatg tttcaaatat ttgtgttgcc
    10561 ccgagtttgc gcaagctcat cgattcatgc gatgaaaaaa taaatgcaaa gggactggtg
    10621 cttatgcccg cctttgttga ctggcatgca cattttcgat atccggggca aggtgaaaaa
    10681 gaagatttgg aaactggtct tgctgcagct cgcgcaggtg gttttagtac ggtggttctt
    10741 atgccaaata caaaccctgt agtttctagc caaaaagaag ctgtttcaat tatggatgaa
    10801 ataaaactgc tcagtgatat aaaagccttt caaacggtga gtattacgaa aaactttgat
    10861 ggaagggata tctcgcactt acatgccctc gacaaatcaa tagtaccaat tattactgaa
    10921 gatggtttcg atgttgcaag tgaatctgtg atgagagaag cgatgaaaat agctgcaaaa
    10981 aaaggcttcg tcgtttcttg ccattgtgag gacgctgcct ttgtcccgct tgcaaaaaaa
    11041 gcccgtgaag aagggaattt taaaaaggca gaaaaacttt tgcaaaaggc ggaggatacg
    11101 gcaacgaagc gaaacctaaa actcgccttg gaaacaggct gccgagtgca tattgcccat
    11161 tgtagtacaa aaaaatcact ggcttatgta agacaggcaa agaaaaaaga aacattcccg
    11221 gggcaaatta cccttgaagt tacgccccat catgcttgct tgagttatga aatgaaggga
    11281 ctcgataagg aactcgtaaa cccgcccttg agaagtgaaa aagacagacg agctgtggta
    11341 aaggcgattc aaaagggtct tgtagatgtg attgcaactg accatgcgcc gcatacactt
    11401 gaagataaaa aaaacggagc ctgtggtttt tcaggaattg aaaccgcttt ttcactttgt
    11461 tataccgaac tcgtaaaaac agggaagctg agcctttcaa aattgagtga actcatgtct
    11521 tcaaaccccg ctcaaatgct aggtttggac aaaggcttta tcacagaagg actagacgca
    11581 aatcttgttt tgctcgatct tgaagcaagc ttttttattg attcaaaaga tttcaaatca
    11641 aagggaaaat atacgccgat acaaggaaaa aaattatttg ggcgtataat ataaaaaaag
    11701 gtatcgcttt tcgtctttta aagtgttatg ataaatatca tgtataaaac attaattttc
    11761 cgtagccttt cacgccacaa aaaaaagggt cgtcgccttt ttgtcttgat ggctctttgt
    11821 tctgcaacca ttattttatt attgtccttt aaagaagatt ttaaaagaca aagcgaagag
    11881 caacttgtgc aacttcgtta tggacattta gagattgtgc caccagattc acctcttgct
    11941 caaaaaaatc ttataactcc accgactgaa aatcttagtt ttattaattt tgataaggat
    12001 tttgacattt ggctccgttc tttaccagag gtagccgaag cttcaccgag cataactcgt
    12061 tatggaattg catatagtct tggaggagaa actgagtcat ggcttgcctt acttgcggtt
    12121 gatgcaaaac ggcttgcctc cttgttgcca cttacgaagg ctctggaagg aagtgatgat
    12181 ataagctgga cagaaggaga agatgttcca atcttacggg ctacacctta ttcgattttt
    12241 ggatcataca ctgaaaatcc cgatattttt tatcgctatc aattaagagc aaacgatgaa
    12301 gggttgcctc agttttttag tgaaatatca aaagattttc caaaaatatt tatatctcct
    12361 ccctatcaaa atgcggcaga tgattggaat cgatttttaa cagagtggaa tgcttttttg
    12421 ataaaagaag atgtacacga atggcttgat gaaataccag agcgtttttt tgaagactat
    12481 gactggaaac tcgatgatgc tatctgggaa ttaagagaga acacaaattt taaacgaaaa
    12541 gcatttttaa agaaaaaagt ctttcaagct ttgtacccaa acggtattgt agaattgcgg
    12601 gaacctatat atgttgacaa acaagttacc ctccagttac cccttataaa tgcaaaagaa
    12661 gtcatagcct tacctgtagc agtcaatgca atgtacaagg gtttttgcgc tgtagaccca
    12721 ctttatcaag aagtgagttt tatggatatt aatgcatttc gttactatat gggcttaaaa
    12781 gaaaatgagg caacagaata tattgttcgt ttacataaaa gttctgacgt agaaaaaata
    12841 aaagcagtaa tagaagacaa gcttttagag atggggtcag atgcaaaagt agttgactgg
    12901 aaaagcttag gtgagatgca aatgacaaca gcaacagcct ttacctttgt tattgggctt
    12961 ttaataggta tatttattat aattgtgctt atttttacag taaatcttgt gctgatatcg
    13021 cttctacagc gaaaaaaaga aataggtacg ggcttagcta tggggcttag tacagatcaa
    13081 acaaggctgc ttatgtctgc agaagttggt ttaatagcct ttgtttcctg cttcctaggt
    13141 tccgtttttg ctcttgtctt aatcgctttt ttttcaaaaa taggtattcc tggcatggtt
    13201 ttttttgtct ccggaaagct ttttttacag tttcggtttt atccatttat tatggctttt
    13261 tttattttag tgccatctac tatgctggtt tctttaattc ctctatcgca actacatgaa
    13321 aagatgcccg tagatttttt caaggagggt aaataatgaa actcgcacta caaatagctt
    13381 ttagaaatat tagctcgcac aaacgtaaaa atcgcattat ttttgctgtt acaacggttt
    13441 taagtttttt tatgtttttg tttttggcat tttctgatgg agagatagaa aacataaaaa
    13501 acggggtttc gagtttttat agaccacact gggacttgcg cgtttttaca aaagagttta
    13561 ttagacttga tcaccgaggc gaaaatcctt gggaggaaac aattttaaat gcagatgagt
    13621 taaaaaaaga cttgttagca tttcctgaaa ttaaagaggt gatatcctat cttccggcaa
    13681 acaatgtttc cttgttcata aatggtaaaa aatatttgta tttttctccc attgcagtag
    13741 agaaaaatga ttcatttatc caatcgaagt acgaaattgt tgagggaaat agagaagtaa
    13801 ttcctgaaaa aaacactatt agtttgcact atatgatgaa aaaactaata ggtgcaaaaa
    13861 ttggtgatga aataaaaatt gtaggtgata ctatttttga tcaagtaacg atggcaaagg
    13921 tgagagtttc gtcgttttat aaaccatatt ttgacaatcc aaatttagca tcatcatttt
    13981 taatgccttt tgaagattta caaaattatg tgggttatga acaaaatgaa gccccatact
    14041 taataattcg tttgcaagca aaagtaaacc ccgaaaaaat gcaaaaacag cttatttcat
    14101 gggcagagga tactaacaaa gaacttcgtt tttttacaca aaaaacgtca agcaggcaag
    14161 atcaatgggc tatgatatat ggtatgattc gctacattat tattgcaatg acgcttataa
    14221 cgttttttat agcggccttt ggtatgatga atgttgtttc tgtaaatctt ctagaaagag
    14281 ccaaagagat aggcacctat tattgccttg gttgtgaaaa accattttta atggctgtat
    14341 atactataga aatttttctt gtaaatatga ttggaggcat agcaggaatt ttacttgggc
    14401 aaggagctcg tttaattgta aatgctctaa aaattacatc tgaagagccc ggttttcaaa
    14461 ttgtggttgg ttcatctgtt ttttatctcg gttcatcttt gagtacagtt gtttggataa
    14521 tcgggggcct ggctataatt actattctta cttctctaag caccttgggt aaggcattga
    14581 aggtttcacc cgttgttgcg gtacaagaac tgaattaaaa taaaaggaaa aaaaatgaaa
    14641 aataaaatat ttaaaacaat tagtataaat ctttgcattc taagtgtaag ctttgcgttt
    14701 ttatatggac aaagtgcaga agagataata aaaaagatgg aagagaaaat gtttccctct
    14761 tctcgctcag aaatgacgct tcgatttgag acaaaaacaa gtcaggaaga ctatgttttt
    14821 acgagttttg ctcgagatac taatcaaaaa attattgtac gatttaaaag ccctgctgct
    14881 atgctcggct cagaccttct catgcttgat cgaaatgttt ggctttatga tccaaagtca
    14941 ggtcgcgaaa tgaagattcc gtcaaaccaa agttttgggg gaacaggttt ttcctatggc
    15001 gatattttac gtttaaatta ttcagataat tacagcgcaa gcataaagga agatttgagc
    15061 gatagctgga ttattgactt agttgcaaaa caaaaagatg caccctatca tgctatttcc
    15121 ttagagatag caaaagattt tacccctgtt ctaggaaggt attttactcg ctcaggagac
    15181 ttaattaaag aaatggagta ttcaaacgct aagttaataa acggtgtttt aaaaccactt
    15241 accgtaaggg taacttcacc cctagatcca agcgaggttt ctacacttag cttggtgaaa
    15301 gaagaaaaaa aagagtatcc tctttcaatt tttaataagc gaaatctttc tctaaagatg
    15361 gaagaaaatt actaacaaaa ggaaaaaaac atgacattta tagaaacaaa agatttaata
    15421 aaaaattatg agcttggaaa aacccttgtg ccagctttac ggggcctaga tgttgcaatt
    15481 gaaaaaggag aatacctttg cgttgcggga ccttctggtg caggaaaatc tacctttctt
    15541 aatttggttg gcttacttga tgaggcaaca agtggtacca ttatatttga cggtacagac
    15601 attactagcc ttagtaaaaa aaactatcac cgatttagac gcgggaaaat atcatttatt
    15661 tttcagtcgt ttaacctcgt tcctgtttta aacgcacgag agaatattga gtttcctctt
    15721 attattcaaa aaattgataa ggccgaacga gacagaagaa tagatgctat taccgaagaa
    15781 attggtattc gacaatggct tcatcataag ccagatgagc tttctggcgg acaaaggcaa
    15841 agagttgcta ttgcacgcgc cctcgtaaca aagcccgagg taataattgc agacgagcca
    15901 actgccaacc ttgactcagt taccggtaag gctattttag acttgcttcg aaggctaaac
    15961 agcgaggagc aaacaacttt tattattgcg agtcatgacc attccttaat cgatggtgct
    16021 gatcgagtta taaaaattgt tgatggtttg gtagcttcat gaaatccttt gttgttcgcc
    16081 ttcattataa aaaaagcttt tttctcctgc tcttgttctc tttcgctttt acgattttag
    16141 ctgcgcaaga aacggatgca gaaaaaaaac ttgaaacaaa gcttgaaaca ggctttgctt
    16201 ttactgccta tgagccttca atcgattcaa cctggtttac aaaagaaagc ctttatcttg
    16261 attttacact aacaagcgga aatatcttaa aagcaaacgg ctttcttgaa gcggtctatg
    16321 atgatgaaaa taaggcaccc gagcctttga cggcagaaga gcttataaaa gagctctctc
    16381 tttcctggat gataagcccc tcttttatag ctacagtagg aaaacaaaaa cttgagtggg
    16441 gtgtcgctcg agcttttcct tcaatcgaca gtcttgaagc cttaacttca gtttctgagc
    16501 caagcgaaaa agcaattcca cgaggcttaa caggatttaa actggatctt attccatctt
    16561 ggttttggtc tttttccttg cttgctgttc cagagcctcg tctaaggga
//
LOCUS       DAOSSW010000033        16265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_3681_length_16265_cov_6.579210, whole genome shotgun sequence.
ACCESSION   DAOSSW010000033 DAOSSW010000000
VERSION     DAOSSW010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 16265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16265
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3681_length_16265_cov_6.579210"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..568
                     /locus_tag="PKW26_04270"
     CDS             224..568
                     /locus_tag="PKW26_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOQ92816.1"
                     /translation="MNDETIITSSAVGKRLDSFSESDKVSYLVFNNSKVRLISKIRIG
                     RANDNDIVVDNKLASRYHAIIQKIKDAYYLKDEGSTNGTFLNGKKIPEAKYFKLQAGD
                     KITIGNSNLVIS"
     gene            604..1554
                     /locus_tag="PKW26_04275"
     CDS             604..1554
                     /locus_tag="PKW26_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOQ92817.1"
                     /translation="MIKPILPSKEELSFLLHSLIDILLNEDEGYRPWYKREVGSLLEF
                     SDSLSVIIIPDLHARFYFLLSLLDYKIEGETIRSLLEQEKIRIIFLGDGLHTEALQKE
                     RWIKAYEAFLQGNLINPFIKEEMAEGLGLMAEVMRLKIAYPALVHFLKGNHENILNEE
                     SYGNRPFRKIVMEGEMVKAFMLSYYGKELTELYASFEFLLPLFVIGEGFLISHAEPAR
                     AYSKKELIEAKKYPSVIHGLTWTKNDEVSGQAVIKLLKKFQRAYPHARYFVGHRPVEE
                     AVAYRAGGKLVQIHDANVNQFVYLKPGELFDDKKNLLIIE"
     gene            1563..3125
                     /locus_tag="PKW26_04280"
     CDS             1563..3125
                     /locus_tag="PKW26_04280"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOQ92818.1"
                     /translation="MAKISPEKIGKYKVVSVIAKGGMGIVYKAIHPTLKRYIIIKKLT
                     MRGNPELRERFKREAEILLDLRHRNIVHMFDYFVEAGSHYIALEFVDGMSLDKLLVKT
                     KKLSCQTAMLIFRDACKALLYAHENGIVHRDIKPGNILISSHGEIKLADFGIASSEKE
                     AETNLTQDGSTLGTPSYMPPEQFSETKKVDNRADIYAMGVMLYEMVTGKKPFSGSFSP
                     ETILQIQKGKFTSPEKIEASVDRTIKKIIKKTVKPNPKKRFKSVKEMLDLADIYMSRY
                     DSEEIRKDLEQKIRLKKVQEAQFKKRFSPLPFLFLFFLTALFGFGVHRSWKEGYIHKY
                     LLFSWYKPVSLELQLTNLEKSKMDLPMRAYFFINDNDEIPEVKNTMRVFEIQKKDSKL
                     RTKPVYLRPGNYRIKVVIGSYIWWQSLEMEAEAQNIHLDFLKNKNRSLEIHVNALDEN
                     LLPLKENLRAEVLLSKGWTDISEIQQGDLQSGTIVKVRIVAKGYETQVFSLKIEWYQD
                     ELFITARMKKKN"
     gene            3209..5716
                     /gene="lon"
                     /locus_tag="PKW26_04285"
     CDS             3209..5716
                     /gene="lon"
                     /locus_tag="PKW26_04285"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOQ92819.1"
                     /translation="MADQTIVPVEQTLSNKLFLIPLVGRPIFPGIFTPLMINSPEDAK
                     AIEESYTSDGLIGIVMLKEDVDNPQVSDMHSVGTVARIIKKINLPDGGVNVFISTIKR
                     FKIKKILNEMDPMVAIVDYLDDEVENNSFEIKALTRALISEMKEISENNPLFSEEMRL
                     NMVNIDQPGKIADFITSILNIEKEEQQRVLEMINIRQRMETVLVFIKKEQELLRIQKK
                     IQTELNERIEKSQREYFLKEELKAIKEELGMTTDAKTSDYQKFKEKLESFHFEGEIKE
                     TVESELEKFSLMDPNSGEFIVTRNYLETIASLPWADESFEEYNIEKAQNILDEDHYGL
                     DDVKKRIIEYLAVRKLKKDNKGSIILLIGPPGVGKTSVGRSIARAMNKEFFRFSVGGM
                     RDEAEIKGHRRTYIGAMPGKIIQGLKIVKTKSPVFMIDEVDKISMSMQGDPSSALLEV
                     LDPEQNISFRDHYLDLPFDISNVLFILTANTLDSIPYPLLDRAEIINLAGYIDQEKIE
                     IAKKYLIPKSLEKNGLKRSQVKYNKKALLTIVNSYAREAGVRNLEKSFDKIHRKYVTE
                     LVSSPETLDLSTRIDVEKKRVEDLEKEKGAEQKTAVNSKKKMPSKMSETEKELQKEYQ
                     KIVGQSLSINEDLVEKYLGKPLFNEDEIKRADRPGCALGLAWTSMGGDTLIIEAIALQ
                     GKAGLQITGQLGDVMKESANIALSWVKQYALKHALVDEKWFENKHIHLHVPEGATPKD
                     GPSAGITMAIALLSLIKNKPIKKNIAMTGELSLTGQVLAIGGLREKTVAAIRNQIKQI
                     IIPQSNLRDLDEIPEIVKNKVVFSPVRSFEEVIDLLF"
     gene            5737..7092
                     /locus_tag="PKW26_04290"
     CDS             5737..7092
                     /locus_tag="PKW26_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOQ92820.1"
                     /translation="MDKKNVFATDETAEEVKSLNQIPQELFQKYEVKRGLRDISGQGV
                     MAGLTRISEIHSYVMSEGDLIPCEGKLYYRGYDIEDLVQGFSDENRFGFEEIAYLLLF
                     GKLPNQTEYDAFREEIASMYSLPKEFLNATIMSFPSRDLMNSLAKSVLTLYAFDENAE
                     DTSIANVIAQSLRLIAQFPSLLVYAYHSFAFAHKKQNFVIKSPKKEYSIAQNILYMLR
                     SSDTFTEHEAKVLDLALVLHAEHGGGNNSTFTTHVVSSSGTDTYSCIAASLASLKGPK
                     HGGANIKVVQMFEDMKKNVKNWTDEEEVNAYLNALLDKQAFDKSGLIYGIGHAVYSES
                     DPRALILKSYVEQLAQEKGMVKEYKLFSIVERLAPLAIAEKRKMYKGVSANIDFYSGF
                     VYKMLGLPDELYTPLFACARVVGWSAHRLEEIANNGKIIRPAYKAIEKHRAYPSIDMR
                     E"
     gene            complement(7175..7726)
                     /locus_tag="PKW26_04295"
     CDS             complement(7175..7726)
                     /locus_tag="PKW26_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ92821.1"
                     /translation="MDYFKELRSDENDSLFDKTNMLYKEFPSDFRQIRYFTLLIVQSA
                     PLEIKERNLLEQQISEVIKNAVKHGNECDINKKVRVWYSFSATHAHLIVEDEGEGFQD
                     LEKWNEFNRKRLECLHNQNFEELAEFVSFRTPKSDDHDGGNALFAALEYWNGGFVYNK
                     KRNGVAMLKKFVQRRLGVSIDGE"
     gene            complement(7746..8084)
                     /locus_tag="PKW26_04300"
     CDS             complement(7746..8084)
                     /locus_tag="PKW26_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521480.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HOQ92822.1"
                     /translation="MELKIRKNGEIYIIDVNGEMDLYNSYKLKELVMKMLEKSVTSFI
                     INLEQVDYIDSSGIGALIYICSTIKKMNLKLAISNIHGSVKKVIELTKLMGYFPIANS
                     VEEALLMVTE"
     gene            complement(8100..9755)
                     /locus_tag="PKW26_04305"
     CDS             complement(8100..9755)
                     /locus_tag="PKW26_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOQ92823.1"
                     /translation="MFIFSDTVSFILLLVSIIPILISIFLIFRITRKAANKNPINQFF
                     SLLILLLGIVAAIIFQSAIFLLSALSLALVLMIPHIVYHLKELKLEKEKTESAENTEI
                     DLEQEVEQIREKDKELNKILFELSNDIIVEASNNLSSEKGLQETLEHINTAIIEEINA
                     DGGVILLIDAFDDIIAVKSLIGSFPPPYELSADIPHKIIRVETNFRFASFPLDSTIFG
                     EIARTGRAELITEPLSDPRLFQNEPEDFLKLGSYIFLPLLVKDSVLGVAAFARKFENP
                     CFTEEEFYKAEILARFASSAIRGVFSYQELAERNELTKEADMAVKIQERYHPKLLPAI
                     PGLSLGHYYNTAGVCGDFFDIVPSRKDRISFVLADVAGKGMNSLLIMTMLRSILRLVL
                     NTTHTASTILSWANRGISTEKTIDHFASIALVLYNSIDNTIQYANAGTSPIYLLRNQT
                     GEFECLSKNSEPIGVEKTSVYSDNSTKVEKNDIIITFTDGLIEALDSSGNQYTTSRLT
                     RLIKQNKDLTGKEIANKIREDMKRFSGDTKQHDDQTLLVIKIL"
     gene            complement(9818..10711)
                     /locus_tag="PKW26_04310"
     CDS             complement(9818..10711)
                     /locus_tag="PKW26_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOQ92824.1"
                     /translation="MSTHSLQSSNDNFFRARNRALINDLQHILSPEETKLLSFNDVKK
                     MLKPNNEVYLGMKVVPIKLIVGSEGRYMDFDSKFFPRKTHLKTRWARIDQAYYKDIIL
                     PPITLYEIGGLYFVRDGNHRVSVAKIKGVENIDAEVISLQSEIKLKPSSSIKEILKQV
                     IQYEKRVFYAETNFGDITDCWTIDFSSPGQYDVLYQHILIHKYYLNQNKTEEIPMHKA
                     VQSWYEQLYLPIIDSIKKQKLLRSFRNRTLSDMYVWLIRYWDELKQQFGETISIEEAA
                     NDFSKIYKKSAIKRILEKFTI"
     gene            complement(10720..11376)
                     /locus_tag="PKW26_04315"
     CDS             complement(10720..11376)
                     /locus_tag="PKW26_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOQ92825.1"
                     /translation="MKILCISDQIDPLIYSNNAKERFQDIDLVLSAGDLPMEYIDYVV
                     STLNRPTLFVFGNHNLKNFNRFHGGTRTKKRTTIDDSTTHLAGAAYVGFKVLKEGKLL
                     IAGASGSLEYNKGESQYSDGEMNRKLIKMIPRLLYNKLRYGRYLDIFLTHAPPLGIHD
                     KEDRCHTGFKSFLWFMRIFKPKYLIHGHIHLYDLQDIRVSVYEQTTIVNAFSHHIIRF
                     "
     gene            complement(11385..12752)
                     /locus_tag="PKW26_04320"
     CDS             complement(11385..12752)
                     /locus_tag="PKW26_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ92826.1"
                     /translation="MLAIQFIILVLMGSSVIFLSYMIFKSILSPKKIENIQKLIKQGK
                     YPAAIKTAKAILSKNPRDFKAHYFLGKAYLLDGKPELALMEYKIVNQTAIFDSSFSET
                     EFRKQISQLYYKYNQPDEALKEYLLLIKLEPQVSEHYYNAGKIFESRSRSEQALAHYQ
                     KAISLDKRNVKAHAALGLLLYRAKQYGEAKKAIDYAISLSPQTYSSYYYLGKILKEGK
                     DYAGAIKAFEKAMRDTEFRQRALIESGTSYMAVNQTEKAIIEFDRALSSASDTNSQES
                     LYARYFLASCYEKERKIDKALEQWEKIYQINHTFRDVPAKLAQYRDLHTNDNMKEYLT
                     SNAEYFIEICKKIALTGYNLEPRSIKSKKFGCIFIATEHKAENWQNVRQQVSLMFFYR
                     ETELIPDSLLRQQLEEMKKQNYVKCIICTSSGFTRSSIEFAENRPFELVGKDKLEAIL
                     SKINL"
     gene            12880..13620
                     /locus_tag="PKW26_04325"
     CDS             12880..13620
                     /locus_tag="PKW26_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ92827.1"
                     /translation="MKYKLRFTVFFVFFLSLMTILFSDSFTEAEDAFRANKMSEAIAL
                     FEKHLQEPNPNPLSYNFLALAYYQRDDKKKALEVFLSGTEKPFTDKRLLYYNAGNVAF
                     SMGLYTDAERYFSLAKSADPEFASALINRANTRVKLTKLLLAIEDYQAYLVLEPLSQQ
                     RAKIEQMIALLENELENQASAEERRVIEEARIKEEQERLAEKDKEDALRRQKLMEEIA
                     ASLQNTESANLRAGTEGVMEYDYEFELD"
     gene            13601..15580
                     /locus_tag="PKW26_04330"
     CDS             13601..15580
                     /locus_tag="PKW26_04330"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ92828.1"
                     /translation="MSLNLIDRRVVLGFLALIVLALAIFIPVRCSQVQKQALVNEREK
                     ERENTLVLVKKYVEKGEYERAMNLLDDLLIKNPHDEEALLFIDEIVQLKSGASLEDFP
                     RYAQSIDLDTSELERAIGKMEEALRQQQAQQESVTKRQEDLAKMQAEELAEERKRKEQ
                     QELEAQKQKAYEEALAKESKELQAQIEKVNDEIALGKAKIGSGKTDEGLVHFNRVESL
                     LPKNQDAFNAKKLSEIANVLYDSSLTQMDVKKKDVLQKESQKFAEKALRFDKNDALAH
                     YLLGKMFSDAKQFDKALEELTLAVQKEPSNYLYFYDLGKVQYSLRQYSEARSSFETST
                     KLQPSFEQGFFNLATTYKTLGRSEEALQAYRRAVTISPNYDRAHLEIARLLALKNDTR
                     GAETSYKKVLEIDSSNASALRELGSLYAQMGKNAEAETSFRKALSFNANDAVTSYNLS
                     TVLYNQAKYDDAGKFAGFAADAQASNASFQYNLGLISEAKGDVDTALVYYSKTLKIDP
                     KHSRANINLGKIFLESGDIESALLCLKTATQTDPKSFEAVNNLASAYLAKKDYAEAIQ
                     NFQKAVKLEPENAMVKMNLARAFASAEDFENAKALFLEILQKDNQNWDAYIEIAKVCI
                     ALKDASAAEGFLLTLQSKNASYKKDEVSALLASIR"
     gene            complement(15627..>16265)
                     /locus_tag="PKW26_04335"
     CDS             complement(15627..>16265)
                     /locus_tag="PKW26_04335"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92829.1"
                     /translation="PIKRSHIDSSFQNEQEFNRSATKKNGLEEKTAASFRYFGSYASL
                     FLIAEKNEKLYIIDQHAAHERILFNQFMQKKAQRQSLLIPQLFTTKDKNEDSYLESIQ
                     NELGEQGFQIEKVAEGKWEIREIHELWEGDLEGLFSEILKNKTEPRDLLRKIAENYAC
                     KKAIKDGSYLDDASAIALITESFSLDDPHCPHGRPIWTIISKEELFSRVKRS"
BASE COUNT         5298 a         2745 c         3149 g         5073 t
ORIGIN      
        1 acatagccta tattatacat gaaaaaaata ctaatacaag aaaaaaacac aggattttaa
       61 cttaaaggtt aaacgaaagt gatattgtcc taaaaaaatg tagggcaatt gccttttata
      121 agaaaatgga ctttgttgaa ttgcaaaacc ttgaagaaat ttttattctt ggaaaaaagc
      181 tttaaaactt gcacgaatct ctacttcagt ttatactatc agtatgaatg atgaaacaat
      241 tataacaagt agcgcggttg gcaagcgcct cgattctttt tctgagtcag ataaggtctc
      301 gtatttagtt tttaataata gcaaagtacg tcttatttca aagattcgta tcggtcgtgc
      361 aaatgataac gacattgtcg ttgataataa acttgccagt cgctatcatg caataattca
      421 gaaaataaaa gacgcctatt atcttaaaga tgaaggttct acaaatggta cttttttaaa
      481 tggaaagaaa atacctgagg ccaaatactt taaactgcaa gcaggcgata agattactat
      541 aggaaatagt aatttagtta tatcctaggc aaaaagagta ttttattgaa ttattcgaat
      601 actgtgataa aacccatact tccttcaaag gaagagcttt cttttctctt acatagcctt
      661 atagatattt tgctaaacga agatgaagga tatagacctt ggtataagcg ggaagttggc
      721 tctcttttag aattttccga ttctctttct gttattatta tcccagattt acatgcgcgt
      781 ttttattttc ttttatcctt attggactac aagattgaag gcgaaacgat tcgctccctc
      841 ttagaacagg aaaaaatacg gattattttt ttgggagatg gcttgcatac agaagctctg
      901 caaaaagagc gatggatcaa ggcctatgag gcttttttgc aagggaatct aataaaccct
      961 tttataaaag aggaaatggc tgaaggcttg ggcttaatgg cagaagtgat gcgcttgaaa
     1021 attgcttatc ccgctttagt tcatttttta aagggaaatc atgaaaatat attaaatgag
     1081 gaaagttatg gtaatagacc ttttcgaaag attgttatgg aaggtgaaat ggtaaaagca
     1141 tttatgcttt catactatgg aaaagagctt acagaattgt atgcaagttt tgagttttta
     1201 ttaccacttt ttgttatagg agaaggtttt cttataagcc atgcggaacc agcgagggct
     1261 tatagcaaaa aagaacttat agaagcaaaa aaatatccct ccgttataca tggactaacc
     1321 tggacaaaaa atgatgaggt aagtggacaa gcggtaataa aactgcttaa aaaatttcaa
     1381 agggcatacc cccatgcccg ttatttcgta gggcatagac cagttgagga agctgtggca
     1441 tatagggcag gtgggaaact cgtgcaaata catgatgcaa atgtaaacca gtttgtgtat
     1501 ttaaagcctg gtgaactttt tgatgataaa aaaaatctct taattataga gtgaggaaga
     1561 aaatggcaaa aataagtcca gaaaaaatcg gaaaatataa ggtagtctct gttattgcaa
     1621 agggtggtat gggaattgtc tacaaggcaa ttcacccgac tttaaagcgg tatattatta
     1681 taaaaaaact gacaatgcgc ggtaatcctg aattgcgcga gcgatttaaa agagaggcag
     1741 aaattctgct cgacttacga catcgcaata tagtgcatat gtttgattat tttgttgaag
     1801 ctggttccca ttacattgct ctcgagtttg tcgacggtat gtctttagat aaactcctcg
     1861 ttaaaacaaa aaaactctct tgccaaacag ccatgcttat ttttcgcgat gcttgtaaag
     1921 cgcttttgta tgcacacgaa aacggaatcg tacacagaga tattaaacct ggaaatattc
     1981 tcatctcttc tcatggagaa attaaacttg ccgattttgg tatcgcttcg tcagaaaaag
     2041 aagcagaaac aaatctcacc caagatggct caaccttggg aactccgtca tacatgccac
     2101 ccgaacagtt ttctgaaaca aaaaaagtgg ataatcgagc agatatttac gcgatggggg
     2161 ttatgctcta tgagatggtt acggggaaaa aaccatttag tggatccttt tcgcctgaaa
     2221 caattttaca aattcagaaa ggaaagttta catcacctga aaaaatagag gcgagtgtag
     2281 acagaacaat taaaaaaata ataaaaaaaa ccgttaaacc gaatccaaaa aaacgcttta
     2341 aaagtgtaaa agaaatgcta gacctcgctg atatctatat gtctcggtat gactcagaag
     2401 aaattcgtaa agatttagaa cagaaaatac ggcttaaaaa agtgcaagaa gcgcagttta
     2461 aaaaaagatt ttcacccctc ccatttcttt ttcttttttt cctaacagcc ttatttggct
     2521 ttggcgtaca taggagctgg aaagaaggct atatccacaa atacctttta ttttcctggt
     2581 ataagcctgt gagtttggaa ctacagctga caaatttgga aaaatcaaaa atggatttac
     2641 cgatgcgggc ctattttttt ataaacgata atgatgaaat cccagaggtt aaaaatacta
     2701 tgcgtgtttt tgaaattcaa aaaaaagatt caaaactgcg tacaaaacca gtgtatttgc
     2761 gaccgggaaa ttatcgaata aaagttgtga ttggctctta tatttggtgg cagtcattag
     2821 aaatggaggc agaagcgcaa aatattcatt tagattttct aaaaaataaa aaccgtagcc
     2881 tagaaataca tgtaaatgcc ctagatgaaa acttactacc acttaaagaa aaccttagag
     2941 cagaggtttt actctcaaaa ggttggacag atatatcaga gatacagcaa ggagatttac
     3001 aaagcgggac tattgttaag gtaagaattg tagcaaaggg ctatgaaaca caagtttttt
     3061 ccttaaaaat agagtggtat caagatgaat tatttataac cgcacggatg aaaaagaaga
     3121 attaagagca aatagttttt acttttccct tgattctttt ttttattttt agcatattta
     3181 aatataagcg aatttttgga ggtacaccat ggctgatcag acgattgttc ctgtagaaca
     3241 aactctttca aataaattat ttttaatacc acttgtagga agacccattt ttcctgggat
     3301 ttttacccca ctgatgataa attctcctga agatgcaaag gcaattgaag aatcctatac
     3361 cagtgatggt ttaatcggaa ttgttatgct caaagaggat gtagataatc ctcaagtttc
     3421 agatatgcac tccgttggaa ctgttgcacg aataataaaa aaaattaact taccagatgg
     3481 cggcgtcaat gtttttatct caacgataaa gcgatttaaa ataaagaaaa ttttaaatga
     3541 aatggatcca atggttgcca ttgtcgacta ccttgacgat gaggttgaaa acaattcctt
     3601 tgaaataaaa gcacttacac gagccctcat cagcgaaatg aaagagattt ccgaaaataa
     3661 tcctcttttt tcagaagaaa tgcgcctaaa tatggtaaat atagaccaac caggaaaaat
     3721 agcagatttt attacttcta ttttgaatat tgaaaaagaa gaacaacaac gcgttttaga
     3781 gatgataaac attcggcaaa gaatggaaac cgttttggtt tttatcaaaa aagaacaaga
     3841 actcttacga attcaaaaga agatacaaac agaactaaac gaacgtatag aaaaaagcca
     3901 gagagaatat tttctaaagg aagaattgaa agctatcaaa gaagagcttg gtatgactac
     3961 ggatgcaaaa acgtctgatt atcaaaaatt taaagagaag cttgaaagct ttcattttga
     4021 aggtgaaata aaagaaactg tagagagtga acttgaaaaa ttttctttaa tggacccaaa
     4081 ttctggcgaa ttcatcgtta caagaaatta tttagaaacc attgcaagtc taccatgggc
     4141 ggatgaaagc ttcgaggaat acaatataga aaaagcacaa aatattttgg atgaagacca
     4201 ctacggttta gacgatgtaa aaaaacgtat tatagaatat cttgcggtac gtaagttaaa
     4261 aaaagacaat aagggttcta ttattttgct catcggtccg ccaggagtag ggaagacgag
     4321 tgttggacgc tctattgccc gagccatgaa taaggagttt tttaggtttt cagttggcgg
     4381 gatgcgtgat gaggctgaaa taaaagggca tcgccgaacc tatattgggg caatgcctgg
     4441 taaaattatt caagggttaa agattgttaa aacaaagtct cccgttttta tgatagatga
     4501 ggttgataaa atctctatga gcatgcaagg agacccatca agcgcacttt tggaagtttt
     4561 agatcctgag caaaacattt cctttaggga tcattattta gaccttccct ttgatatttc
     4621 aaatgtactg tttattttaa ccgcaaatac cctcgactct attccctatc ctttattgga
     4681 tagagctgaa attatcaatc tcgcggggta tattgatcaa gaaaaaattg aaattgcaaa
     4741 aaaatacctc attccaaaga gtcttgaaaa aaatggctta aaacgcagtc aagtaaaata
     4801 caataaaaaa gcacttctca caatcgttaa ctcctacgct cgtgaagctg gagtgagaaa
     4861 tcttgaaaag agttttgata aaatccacag aaagtacgtt accgagttgg tttcttctcc
     4921 tgaaaccctt gacctttcaa cacgcatcga tgtagagaaa aaacgagtag aagacttgga
     4981 gaaggagaaa ggcgctgagc agaagacagc tgtaaatagc aaaaaaaaga tgccgagcaa
     5041 aatgtctgaa accgaaaaag aattacaaaa agaatatcaa aagattgttg ggcaaagtct
     5101 ttctataaat gaggaccttg ttgaaaaata tctcggtaag cctcttttca atgaagatga
     5161 gataaaaagg gcagatagac ctggttgcgc tttaggactc gcctggacga gcatgggagg
     5221 tgatacactc atcatcgaag caattgcctt gcagggtaag gctggcttac aaataaccgg
     5281 gcaactaggc gatgttatga aagagtcagc aaatattgcc ttaagctggg ttaaacaata
     5341 tgccctgaaa catgccctcg ttgatgaaaa atggtttgaa aacaagcata ttcacttgca
     5401 tgtacccgag ggagccactc caaaagacgg tccctcagct ggtattacga tggctattgc
     5461 cttactttct cttatcaaaa ataaacccat taagaaaaac attgcgatga caggagaact
     5521 ttctctcact ggccaagtac ttgcaattgg tggtttacgt gaaaaaacag ttgctgctat
     5581 tcgaaatcaa ataaaacaga taattatacc acaatcgaac ctccgagacc ttgatgaaat
     5641 cccagaaata gttaaaaata aagtagtatt ttcaccagta cgttcctttg aggaggttat
     5701 cgatctgcta ttttaataaa aaggagtaaa aatataatgg ataaaaaaaa cgtattcgct
     5761 accgatgaga cggctgagga agtgaaaagc ttaaatcaaa tacctcaaga attatttcaa
     5821 aaatatgaag taaagcgagg cttacgggat ataagcggtc aaggtgttat ggctggccta
     5881 acgaggattt ctgagattca ctcctatgta atgagtgaag gagatttaat accttgcgaa
     5941 ggtaaacttt attatcgtgg ctacgatata gaagacttgg tacaaggttt ttccgatgaa
     6001 aatagatttg gctttgaaga gatagcgtat ttgctccttt ttggaaaact accgaaccaa
     6061 accgagtatg atgcttttcg ggaagaaata gcttcgatgt attctttacc taaagaattt
     6121 ttgaatgcaa cgattatgag ctttcctagc cgagatttaa tgaattcact tgcaaaatct
     6181 gttttaacac tctatgcctt tgacgaaaat gcagaagata cttctattgc aaacgtcatt
     6241 gcacaatctt tgcgacttat tgcacaattt ccttcactac ttgtctatgc ctatcattca
     6301 tttgcctttg cccacaaaaa acaaaacttt gtcataaaat ctccaaaaaa agaatattct
     6361 attgcgcaaa atattttata tatgcttcgc tcttccgaca cctttactga gcatgaagca
     6421 aaagttttag atctagccct tgttttacat gcagagcatg gaggaggaaa taactcaacc
     6481 tttaccaccc atgtggttag ctcgagtggt acagatacct attcttgcat tgcagcttct
     6541 ttggcatcgt taaaaggacc aaaacatggc ggagcaaata ttaaagtcgt gcaaatgttt
     6601 gaagatatga aaaaaaatgt aaaaaattgg acagatgagg aagaagtgaa tgcctatctc
     6661 aatgcgcttt tagacaaaca agcctttgat aaatcaggtt taatttatgg cataggtcat
     6721 gctgtttatt ctgaatctga tccaagggcc ttaattttaa aatcctatgt cgagcaatta
     6781 gcacaagaaa aaggtatggt gaaagagtat aaactctttt ccatagttga aaggttggct
     6841 cctctggcaa ttgctgaaaa aaggaaaatg tataaggggg ttagtgcaaa cattgatttt
     6901 tattcaggct ttgtgtataa aatgcttgga ttgcccgatg agttgtatac ccctctattt
     6961 gcttgtgcac gagtggtagg ctggtctgcc catagactag aagaaattgc aaataatggt
     7021 aaaattattc gacctgcata taaggcaatt gagaaacata gagcctatcc gagtattgat
     7081 atgagggaat aagaattaca agccaaaact ataaaaaaat gacctgttaa gaagtatttc
     7141 ttccaacagg tctttttttg tatacgggga gaacttattc tccgtcgatt gatacaccga
     7201 gacgtctttg tacaaatttt ttaagcatcg caacaccgtt gcgtttttta ttgtatacaa
     7261 aaccaccgtt ccaatattct aaggcagcaa aaagtgcatt accaccatcg tgatcatcgc
     7321 ttttcggcgt tctaaatgaa acaaactcgg cgagttcttc aaaattttga ttatgcagac
     7381 actctagacg ttttcggtta aattcattcc atttttctag atcttgaaat ccttcacctt
     7441 catcttcaac aattagatgg gcatgtgtgg cactaaaaga ataccataca cgaacctttt
     7501 tatttatatc gcattcgtta ccgtgcttta cagcattttt aattacctcg gaaatctgct
     7561 gttcgagcaa gtttctttct ttaatttcaa gcggggcaga ttggacaatt aaaagtgtaa
     7621 aatatctaat ttgtctaaag tctgatggga attctttata gagcatattg gttttatcaa
     7681 ataagctatc attttcatct gaacgtaatt ctttaaagta atccatagtc atttccttaa
     7741 ttattttatt ctgttaccat caataaagct tcttcaacac tgtttgcaat tgggaaataa
     7801 cccataagct tggttaattc aataaccttt ttaactgaac cgtgtatatt agaaatagcg
     7861 agttttaagt tcatcttctt aatcgttgaa caaatatata tcaatgctcc aattccactt
     7921 gagtcaatat aatctacttg ctctaagttg attataaagc ttgtgactga tttttcaagc
     7981 attttcataa cgagttcttt tagcttataa gagttgtata agtccatctc tccatttaca
     8041 tcaattatgt aaatttcgcc attttttcgt atctttaatt ccatttgttg ctccctttgt
     8101 tatagaattt taatgacaag taaggtttga tcatcgtgct gttttgtatc tccactaaat
     8161 cttttcatgt cttctcttat tttattggca atttctttgc cagttaaatc tttattttgt
     8221 tttattaaac gagtcaatcg tgatgtagtg tactgatttc cactagaatc taaggcttca
     8281 atcaagccat cggtaaaggt gataattata tcattctttt ctaccttcgt actattatcg
     8341 gaataaacac tcgttttctc caccccaatt ggctcagaat ttttagataa acattcaaat
     8401 tcgcctgttt ggtttcgtag taaataaata ggacttgttc ctgcattagc atactgaata
     8461 gtattgtcaa ttgaattata caatactagt gcaatacttg caaagtggtc aattgttttt
     8521 tctgttgaaa tacctctgtt cgcccatgag agaatagttg aagctgtatg tgttgtattt
     8581 agcacaagtc gaagtataga acgtaacatt gtcataataa gtaaggaatt catcccctta
     8641 ccagcaacat cagctaaaac aaaggatatt ctatcttttc ttgaaggtac gatatcgaaa
     8701 aagtcaccgc aaacaccagc agtattgtaa tagtgcccta acgaaagacc tgggatagct
     8761 ggaagtagct ttggatgata gcgttcttga atttttacgg ccatatcggc ttctttggtt
     8821 aattcatttc tttcagcaag ttcttgatag gagaagactc cgcgtattgc agagcttgca
     8881 aatcgtgcga gtatttctgc cttataaaat tcttcttcag taaaacaagg gttttcaaat
     8941 tttcttgcaa aagcagcaac gccgaggaca gagtctttta caagaagtgg tagaaaaata
     9001 taactaccta gttttaggaa atcttctggt tcgttttgaa agagtcttgg atcggataag
     9061 ggttcagtga taagctctgc tcgccctgtt cgtgctattt ctccaaaaat agtgctatct
     9121 aaggggaaac tagcaaagcg aaagtttgtt tctacacgaa taattttatg aggaatatca
     9181 gcactaagct cgtagggagg agggaagctg cctatcaaag attttaccgc aataatatca
     9241 tcaaaggcat caattaaaag aataactcca ccgtccgcat ttatttcttc tatgatggct
     9301 gtatttatgt gctctaaggt ttcttgcaaa cctttttcgg aagataagtt atttgaagct
     9361 tccacaataa tgtcattgct taattcaaat aatatcttgt ttaattcctt atccttttca
     9421 cggatttgtt ctacttcttg ttcgagatcg atttcagtat tttctgcact ttctgttttt
     9481 tctttttcta gttttaattc ttttaggtga tatacaatat gagggatcat taaaacgagg
     9541 gctaagctta gtgccgaaag taaaaagata gcagattgaa aaataatggc tgctacaata
     9601 cccaaaagta gaatgagaag gctaaaaaat tgattaattg gatttttatt agcagccttc
     9661 cttgttattc ggaagatcaa gaaaatagaa ataagtatgg gaataatgga aactaaaagt
     9721 agtataaatg atactgtatc tgaaaatata aacaaaacta acctcattaa caaaagcttt
     9781 tatcatttta gcattgaaaa atcaagacgt caaggattta aatagtaaat ttctctaaaa
     9841 ttcgttttat cgcacttttt ttgtagattt tactaaaatc gtttgccgct tcttctatgc
     9901 taattgtttc accaaattgt tgttttaact cgtcccagta gcggataagc caaacataca
     9961 tatctgaaag tgttctattt cgaaaacttc gtagtaattt ttgttttttt attgaatcta
    10021 taatgggaag atagagttgt tcataccaag attgaacggc tttgtgcatg gggatttctt
    10081 cagttttatt ttgatttaag taatacttat gaattaaaat atgctgatag aggacgtcgt
    10141 attgacctgg ggaagaaaaa tcgatagtcc aacaatcggt tatatcccca aagttcgttt
    10201 ctgcataaaa cactctcttt tcgtattgaa taacttgttt gagaatttct tttatagagc
    10261 tacttggttt aagttttatt tcactttgaa ggcttattac ttctgcatct atattttcta
    10321 ccccctttat tttggcaaca gaaacacggt ggttaccatc gcggacaaaa taaagtcccc
    10381 ctatttcgta gagtgtaatg gggggaagaa ttatatcttt ataataggct tggtcaattc
    10441 tcgcccatcg agttttaaga tgtgtttttc gtgggaaaaa cttgctgtcg aagtccatat
    10501 agcgtccttc actgcctact ataagcttaa ttgggacaac tttcatgcca agatagactt
    10561 cattatttgg ttttaacatt tttttaacat cattgaagga caataattta gtctcttctg
    10621 ggctgagtat atgctgtaaa tcattaatga gtgcacggtt gcgtgcacga aaaaaattat
    10681 cgttactcga ttgtaaacta tgagtgctca taaaattcct caaaaacgta taatatgatg
    10741 gctaaaagca tttacgattg ttgtttgttc ataaacagaa acacgaatat cttgtaaatc
    10801 atacaggtga atatgcccgt gtatgagata ttttggttta aagatacgca taaaccataa
    10861 aaagctttta aatccggtgt ggcagcgatc ttctttgtcg tgtatgccaa ggggtggtgc
    10921 atgtgttaaa aaaatatcga ggtagcgacc ataacggagt ttattgtaaa gcagacgggg
    10981 aatcattttt ataagttttc tattcatttc cccgtcagag tattggcttt ctcctttatt
    11041 gtattcgaga gagcctgagg cacctgcaat gaggagtttt ccttctttta aaaccttaaa
    11101 gccaacgtag gcagctcctg caaggtgtgt agtgctatcg tcgatggtag ttcttttctt
    11161 agttcttgtg cctccatgaa agcgattgaa gttttttagg ttatgatttc caaaaacgaa
    11221 gagtgtcggt ctgttcaatg tggagacaac gtaatctata tattccatag gcaaatctcc
    11281 agcagataaa accaaatcta tatcttgaaa tcgttcttta gcgttattgc tgtatatgag
    11341 gggatcgatt tgatcggaaa tacaaagtat tttcatagga catcttataa gttaattttt
    11401 gataaaatcg cttctagttt atcttttccc acaagctcga aggggcgatt ttcagcaaac
    11461 tcgatgcttg atcgtgtgaa gcctgaactc gtacaaataa tgcatttaac ataattttgt
    11521 tttttcattt cttcgagttg ctgtcttaaa agactatctg gtatgagctc tgtttcgcgg
    11581 taaaaaaaca tgaggctaac ttgttggcga acattttgcc agttttcagc cttatgttct
    11641 gtagcaataa aaatgcaacc aaattttttt gattttattg aacgtggttc aagattatag
    11701 ccagttaaag caattttttt gcaaatttct ataaaatatt ctgcgttgct tgttaggtat
    11761 tccttcatgt tgtcatttgt atgcaaatcc ctatattgag ctaattttgc aggaacgtca
    11821 cggaaggtat gatttatttg ataaattttt tcccattgtt caagagcctt gtcaattttt
    11881 cgctcttttt catagcaact tgcgagaaag tagcgtgcat aaagactttc ttggctgttt
    11941 gtatcgcttg cagaagaaag agccctatcg aattctataa ttgctttttc tgtttgatta
    12001 acagccatgt aactcgtgcc actttcgatg agcgctcttt gtctgaattc ggtatcacgc
    12061 atagcttttt caaaggcctt gattgcccct gcataatctt ttccttcttt aagaattttt
    12121 ccaagataat aataagaaga gtaggtttgc gggcttaaag atatggcata atcaatggct
    12181 ttttttgctt ctccatactg ttttgcacga tacagcaata aaccaagggc agcatgggct
    12241 tttacatttc gtttatctaa agaaattgct ttttgataat gagcaagtgc ttgctctgag
    12301 cgagatcgcg attcaaatat ttttccagca ttataataat gttcagatac ctgaggttcg
    12361 agttttatga gcaggaggta ttcttttaag gcctcatctg gttggttgta cttatagtag
    12421 agctgggaaa tttgtttgcg aaattcagtc tcgctaaaac ttgagtcaaa aatagcggtt
    12481 tggtttacga ttttatattc catgagagca agttctggct tgccgtcaag aagataggcc
    12541 tttcccaaaa aataatgcgc cttgaaatcc cttgggtttt tcgataaaat tgcctttgct
    12601 gtttttattg cagcaggata cttgccttgt ttaatgagct tttgtatgtt ttcgattttt
    12661 ttgggggaca aaattgattt aaatatcatg tatgataaaa aaatgacact tgagcccatc
    12721 aaaaccaaga taataaattg aattgcaagc attattcctc cgatactaag gatagtatgt
    12781 ttatacaatt atgtcaaata cgtaagaaaa aaagctccta taagagtact tttacttctt
    12841 tattctacca tgtataataa cgacataaaa ggaatacaaa tgaaatataa attgcggttt
    12901 acagtctttt ttgttttttt tctaagttta atgacaatat tgttttcaga ttcatttact
    12961 gaggctgaag atgcttttag agcaaataaa atgtctgaag caattgcact ttttgaaaag
    13021 cacttacaag agccaaatcc caatccgctt agttataatt ttttagcctt agcctattat
    13081 caaagggacg acaaaaaaaa ggccttagaa gtttttttaa gtggcacaga aaaacctttt
    13141 actgacaaac gccttttata ctacaatgca gggaatgttg ccttttctat ggggctgtat
    13201 actgatgctg aaagatattt tagcttagca aaatctgctg atccagaatt tgcgagtgca
    13261 ttgataaatc gtgcaaatac gagggtgaaa ctcacaaaac tattgcttgc gatcgaggat
    13321 tatcaggcct acttagtttt ggagcctctg tcgcaacaaa gagcgaagat tgaacagatg
    13381 attgctttac tcgaaaatga attggaaaat caagcatccg ctgaagaacg ccgtgttata
    13441 gaagaagcac gtataaaaga agagcaggaa cggcttgctg aaaaagataa agaagatgca
    13501 cttcgtcgtc aaaaactaat ggaagagata gctgcatctt tacaaaatac tgaatctgca
    13561 aatcttcgtg caggaacaga aggagttatg gagtacgatt atgagtttga acttgattga
    13621 tagaagggtc gttttaggct ttttggcgct aattgtcctc gctcttgcta tttttatccc
    13681 tgtgcgttgt tctcaggtgc aaaaacaagc gcttgtaaat gaaagggaaa aagagcgaga
    13741 aaatacgctt gtccttgtga aaaaatatgt tgaaaagggc gagtatgagc gggcgatgaa
    13801 tttactcgac gatttgttga taaaaaatcc acatgatgag gaagcacttt tatttatcga
    13861 tgaaatagtg cagctcaaat caggtgcatc tttggaagac tttcctcgct atgcgcaaag
    13921 tattgatttg gatacgagtg aactagagcg agctataggc aagatggaag aagccttacg
    13981 ccaacagcag gctcagcaag aatctgttac caagcgccaa gaagaccttg caaaaatgca
    14041 agctgaagaa ttggctgagg aaagaaaacg aaaggagcaa caagagcttg aagcgcaaaa
    14101 acaaaaagcc tatgaagagg cccttgcaaa agagagtaaa gaactacaag cacaaataga
    14161 aaaagtaaat gatgaaatag ctcttgggaa ggcaaaaatc ggaagtggaa aaacggatga
    14221 ggggcttgtt cactttaatc gtgtggaatc gctcttgccc aaaaatcaag atgcctttaa
    14281 tgcaaaaaaa ctttcagaga ttgccaatgt attatatgat tcatctttga cacaaatgga
    14341 tgtgaaaaaa aaggatgttt tacaaaaaga gtctcaaaag tttgccgaaa aagcacttcg
    14401 ctttgacaaa aatgatgcgc ttgcccacta tcttttgggg aaaatgtttt ctgacgcaaa
    14461 acaatttgac aaggcactag aagagctgac attggctgtg caaaaagagc catcgaatta
    14521 tctttatttt tatgacttag gcaaggtaca gtactccctt cgacaataca gtgaggcgcg
    14581 ttcttcattt gaaactagta cgaaactgca gccttctttt gaacaaggtt tttttaactt
    14641 agcaacaact tataaaacct taggacgaag tgaagaagcc ttacaagcct atcgtagggc
    14701 ggttacaatt agcccaaatt atgatagggc gcatttggag attgcacggc ttctcgctct
    14761 taaaaatgac acacgcgggg cagaaacttc gtataaaaaa gtcttggaga tagactcttc
    14821 caatgcaagc gccttgaggg aactcggttc cttgtatgcg caaatgggaa aaaacgccga
    14881 agctgagact tcttttagaa aggctctttc ttttaatgca aatgatgcgg taacttcgta
    14941 taatttatcg actgttttat ataatcaagc aaaatatgac gatgcaggaa aatttgcggg
    15001 ctttgccgct gatgcacagg cttcaaatgc aagtttccag tataatcttg gactcatatc
    15061 tgaggcaaaa ggcgatgtag ataccgcctt ggtctattat tctaaaacgc tcaaaattga
    15121 cccgaaacat agccgagcaa atatcaattt gggtaagatt ttcctagaat cgggtgatat
    15181 agagtctgct cttttatgtc taaaaacagc gacccaaacg gatcccaaaa gttttgaagc
    15241 ggtaaacaat cttgcaagtg cctacttggc aaaaaaagac tatgcagagg caattcaaaa
    15301 ttttcaaaag gcggtaaaac tggagcccga aaatgctatg gtaaagatga atcttgcaag
    15361 ggcctttgcg agcgcagaag attttgaaaa tgcaaaagcc ttgtttttgg aaattttaca
    15421 aaaagataat caaaactggg atgcctatat tgagattgca aaggtatgta ttgctttaaa
    15481 agatgcatct gcagcagaag gttttttgct taccttgcag tcaaaaaatg caagttataa
    15541 aaaagatgag gtttctgcct tgcttgcaag catacgatag ttggcagatt tatgtcggag
    15601 tttttttaca tggtttttgc tcgtatttag cttcgtttaa cgcgtgaaaa aagctcttct
    15661 ttactgatga tggtccatat aggacgccca tgggggcaat gtggatcatc taaggaaaaa
    15721 ctttctgtga tgagggcaat agcgcttgca tcgtcgagat agcttccgtc ctttattgct
    15781 tttttgcagg cgtaattttc tgcgattttt cgcaaaagat cgcgtggctc tgttttattt
    15841 ttcaagattt cgctaaagag cccttccaag tctccttccc agagttcatg aatttctcga
    15901 atctcccatt ttccctcggc gactttctct atctgaaaac cttgttcccc taattcattt
    15961 tgtattgatt caagatagga gtcttcgttt ttgtcctttg tcgtaaaaag ctgtggaatg
    16021 agcaggcttt gcctttgtgc ttttttttgc ataaactggt taaataaaat acgctcgtgg
    16081 gctgcatgtt gatcgatgat atagagtttt tcatttttct cggcaattag aaaaagggat
    16141 gcatagctgc caaaatagcg aaaagaagcc gctgtttttt cctcaagacc attcttcttt
    16201 gtggcgcttc tgttaaattc ctgctcgttt tgaaaagagc tatctatgtg gcttcttttt
    16261 atagg
//
LOCUS       DAOSSW010000034        15963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_3764_length_15963_cov_7.675698, whole genome shotgun sequence.
ACCESSION   DAOSSW010000034 DAOSSW010000000
VERSION     DAOSSW010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 15963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15963
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3764_length_15963_cov_7.675698"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..812
                     /locus_tag="PKW26_04340"
     CDS             30..812
                     /locus_tag="PKW26_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOQ92830.1"
                     /translation="MQFFDTHAHIGLIYDDPIEQLRVIQEAKQAQVTRIVSICNSLHD
                     FTKVYGNLKGVQGIYHAVGVAPSEVTNPGKNWIDTVENALSLPNVVALGETGLDYYRK
                     FGDKRSQIELFITELELAEKHKKPVIIHNRDAGADVLDILRERIPSAGAVLHCYSENA
                     EYAQRVLDLDLNVYFSFAGNLTYRNAKNLHETIVMLPLDRVMIESESPFMIPAEFRLL
                     KRNMPSYTPSTARFMADMLDIDFEELAAQLWKNSCTFFNLPE"
     gene            820..1806
                     /gene="dusB"
                     /locus_tag="PKW26_04345"
     CDS             820..1806
                     /gene="dusB"
                     /locus_tag="PKW26_04345"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOQ92831.1"
                     /translation="MNALLYKPVSIGSVHIPGNLFLAPVAGYSDRAFRSICIAEGANL
                     CYTEMVSAEALWRGSDKTEMLLLRGENEAFFAPQIFGGEVDSMKKATRILVEKYTPSL
                     IDINAGCPVPKIIKSNAGSALTRDPRHLYAITKAVVDEAGSIPVTIKIRSGWDAKNLN
                     WKETSQAAYEAGAQAICLHARTRAQGYEGFADWQILGELVAFIGKKLPVFGSGDVFSP
                     EAAKEMLEKTGCHALMFARGAMGNPFIFRQTRDLLLSNSYEATRIEDKVMRAMQELDL
                     LIVDKGEELACREMRKRFSAYSKGLEGGGKMRLAIVSATKKADYEEIFKNFL"
     gene            1836..3425
                     /locus_tag="PKW26_04350"
     CDS             1836..3425
                     /locus_tag="PKW26_04350"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HOQ92832.1"
                     /translation="MALLNSIAQFLKRLFHKEKNVHSQAPQLRKIELELKNHPAQIYK
                     NAYITPAFAQVIHILQTETVKMHNFLLETLYNPNRKIAMRYSTSLIETGFTQEQRDLL
                     ESLSYENRKKEYLGKALGVHAEIKTNQEKAIARALSFFDADEFKKIEQVIRNLDHLFD
                     VCQFNYVSFLTQFDQLYSERKDTAPRFSPLLLADAETHLVDLYYLAAGFTLSLAEARA
                     LEALCFVHNGYEQANQKCFLKEVKTIHAAFKNILNADTLQKMIFIIKGKTDVLLQVAT
                     YNRNVIEPYKEEMKKKFDSESKRIAVELQDEKNTEDIKTLFDNRNLLPLQGYTQELSH
                     FLQSNSVEGFLHIVPLSIINTFMRMFLTDRIVSYLNRIVVEGFFTNPSFKSDFSSAVF
                     ACSEIDTKLRSFENTFTKGQTNDLAVLRSNALEGQQNVDLLKKVSFQIDTINHDAQRL
                     MQEIMNDINSLAHIIEVLLSEAKKSNPSDISNIKLLVSSVRNKDAAEELENNFSKWMF
                     FIDIMQNYVIIKDKDRKKLGT"
     gene            3422..4525
                     /gene="dgcA"
                     /locus_tag="PKW26_04355"
     CDS             3422..4525
                     /gene="dgcA"
                     /locus_tag="PKW26_04355"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcA"
                     /protein_id="HOQ92833.1"
                     /translation="MTQTNKPKKKLSLQEKVVLYEKQLYDYRQLLEMSKSLSSVLEID
                     TLIESILYVCMCQMRVLGTGVFILKSFDADFFALDSNYNGLELVDSVSYLIPTTHPLI
                     PFLAENNRTYTYEELESCFKDTSILEQISSLKPSLIIPLKQKNHLNGILLLGERIDVG
                     EGVAYSDYEREQMLDIASISAIAINNASLIEMTTTDMMTRLKLKHYFYTILAEKLDFS
                     FENRLPLGVLMLDIDHFKKFNDTYGHACGDYVLQQVAKKIFEGIRGQDMAGRYGGEEF
                     VVMLYNTEKDAAMLVAERIRKDIEKAELHYEGQDLSVTISIGVAFCPPETSMTAKEIV
                     EFADQALYESKANGRNRVTLAQTYKDNAFAGEK"
     gene            4522..5184
                     /locus_tag="PKW26_04360"
     CDS             4522..5184
                     /locus_tag="PKW26_04360"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOQ92834.1"
                     /translation="MKTNTIIKKPFRYTFKNFAFYLIAINVIVYFFTSMTYRLYRSDF
                     LYSYLSLNVINVVIHKMLWQFITYMFVHGSVSHLFFNMLALFFFGNMLERAMGSKEFL
                     LFYLLTGFLSGFLSFIIYYFSGGHRVFLMGASGAIYAVLLAYAVYFPRNRIFIWGIVP
                     VPAPVLVVVYAGIEFASQFLSLRKGVAHITHLAGFAFAWLYLQVRIGVNPLKVWRNAF
                     HH"
     gene            5168..5878
                     /locus_tag="PKW26_04365"
     CDS             5168..5878
                     /locus_tag="PKW26_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92835.1"
                     /translation="MPFTTNTIKKNLLLCVFLVLVFCCFSQAALMETESAENVLRFSL
                     WSLLEDEPSLEKEALENFYDKSVQELKILAPFLLEGMIYGWSFSYVPSDIARQVAEEL
                     EILPLVRIALPDERLSFLEPRIEDERFYVWLEYRRTNSMMAYKKAWDSVVYPKAKGIG
                     QASLLMGTEGILEAYEQALKNAIRGFVQKQEKNKPRRISGRVLLVGKPMLGIQAGRYT
                     AALDFFVHVIKIERYTEF"
     gene            5897..7444
                     /locus_tag="PKW26_04370"
     CDS             5897..7444
                     /locus_tag="PKW26_04370"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOQ92836.1"
                     /translation="MDIQNIVKNLHPLEIKVIKSFKPGQELTSELLQKELDYKEGHAN
                     QAFSWLSGKELVSVVRKEAFVFYEITELGKSWAENGTPEEKVVAFLKSSGSHSLPEIA
                     KALSIENKDVGSAFGLLSKEGVLKMDATKQASRTGKDSSPRMEMVKKLLEKALASPDG
                     ILDHAHLEKDAQEVMAGLAKKRGASDAAFRIIEKEIVFYSLKDTVEELLSALDAAGIS
                     GNEIGSLTSKMLSTGEWKNANFRGYNISVPPARLIPGRTNPYVNFLESVKDKLSSLGF
                     EEFDGPLVETEFWNSDALFMPQFHAARDIHDAYSLKNPTHAKSIEEPFLTNVAQAHTD
                     GGNTGSRGWNYAFDRDFTRRLILRSQGTVLSAHQLAKAKIPGKYFGIARCFRYDKVDA
                     THLSDFYQTEGIVLGNEVNLKTLLGFLEMFAIEIAGATDVKYVPGYFPFTEPSIEVHI
                     KHPVLGWFELGGSGIFRPEVTKPMGIDVPVLAWGIGIDRMALMALGLNDLRELFSNDI
                     EQVRLRR"
     gene            7459..>7820
                     /locus_tag="PKW26_04375"
     CDS             7459..>7820
                     /locus_tag="PKW26_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92837.1"
                     /translation="MTKRQWQAFEAFRENFRKKCTEWNAKALSLGFPQAQKALAIKDA
                     VPEYPIENSVVYNTKLDTVTKQSDIKLILVSDNPGKDEQREKNKAYLVGQAGKLAEGF
                     FRKNPELGIHFSENLLIL"
     assembly_gap    7821..7920
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7958..8317)
                     /locus_tag="PKW26_04380"
     CDS             complement(7958..8317)
                     /locus_tag="PKW26_04380"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOQ92838.1"
                     /translation="MDTFTMVRSEHLNHYGKLFGGQLLKWVDEFSWLAAKREYKKNNL
                     VTRAMENIEFHHMVPNGAILRFHLEKIKRGTTSLVYSVDVYSEDLAGNPEKHIFSNRV
                     TLVAVDEHGKKTPLIGE"
     gene            complement(8415..9620)
                     /gene="trpB"
                     /locus_tag="PKW26_04385"
     CDS             complement(8415..9620)
                     /gene="trpB"
                     /locus_tag="PKW26_04385"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOQ92839.1"
                     /translation="MSYLKQHPTEDGYFGEYGGSFVPPELQSEMDKITEAYFSISKSH
                     EFISELRSIRKHYQGRPTPVYFCKRLSEIYGGRIYLKREDLNHTGAHKLNHCMGEGLL
                     AKYLGKKKLIAETGAGQHGVALATAAAYFGLECEIHMGEVDIAKEYPNVVRMKLLGAT
                     VVPVSHGLKSLKEAVDSAFEAYLKDPINTIYCIGSVVGPHPFPMMVRDFQHVVGVEAR
                     EQFLEMTGELPDAVAACVGGGSNAMGIFSGFLDDAEVSLIGVEPGGRSLKMGDHAASM
                     SYGKPGILHGFKSYLLQDEKGEPAPVYSIASGLDYPGSGPEHCMLKDKKRVHYEIAND
                     KETLEAFYKLCRYEGIIPALESAHAVAFAMRHAQKNRQKSILVNLSGRGDKDIDFIIE
                     KYGAGDEFF"
     gene            complement(9760..11757)
                     /locus_tag="PKW26_04390"
     CDS             complement(9760..11757)
                     /locus_tag="PKW26_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOQ92840.1"
                     /translation="MQIKKYVLKSLFLFFIFFQLTHLFAEQNEVDSVWFQSSALFIGE
                     EAFSQKIDKLREEREPLALVLSGGSARAYAHIGVLRELEKANIKPDFIVANSMGAIIG
                     LLYASGFSPDDIEAIVTQVDLNSFFDIVFPLRGGILSSRYFLESLSRLFPNKNFDIKD
                     SFIPIIVPTEDLYTKRQVLFAEGEITKIMAASFAMSFMMEPVPYLLESGQNLSLVDSG
                     SIDLGSLKIAKRFTDNIIISTALYDTKLNLNNPVTVLNRTFSIGKERQLVQDILDFEP
                     HLIRNDVEQFSFMDFTKVHEIIGRGEMSTKAFLNAKPEIVHGNFPPDEDVGFQSLRSE
                     RQKSAALFIEEIQRGRKPTSNLPYLGLKIRPNVAVIDFSEFLLAPEPTIGAYAFFDFG
                     PFFTRAGAHTNIFNSFGGDFFSSLHFSSGFETKLSYNYRSPYGDLNQASHYVGLGLSW
                     IFSPLKEVFFKPLFTAELLSDADFREKALFSQLGFSLFNTKDKPYQFSIEPSVFYNTA
                     KLSPEFTKNNLGLTLSFFSKVHPFSKLGFSLTQLMRYTPFAEEAVLFYNDGFRAIYDY
                     SSQANKNSIALSQLNLYWYTKNTGFTAMEAFKLEALSLGLFTDALYDFSFQNHPLFIV
                     GLFSKQEISLAGLSSFNLEAYVGWDFEHAEPKASLRFVSQF"
     gene            complement(11868..12758)
                     /gene="pdxS"
                     /locus_tag="PKW26_04395"
     CDS             complement(11868..12758)
                     /gene="pdxS"
                     /locus_tag="PKW26_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOQ92841.1"
                     /translation="MASERDKKFNQFCKLADSFKGGVIMDVTNVEEAKIAQEAGAVAV
                     MALERVPSDIRVQGGVARMSDPGMIIEIKKAVKIPVMAKARIGHITEARVLEAIGVDF
                     IDESEVLTPADDQFHIDKKVFNVPFVCGCRNLGEALRRIGEGAAMIRTKGEAGTGNVV
                     EAVRHMRTVQAEMRKLKSMAYDELMVYARDIAAPYHLVEFVAKKGKLPVANFSAGGVA
                     TPADAALMMELGSEGVFVGSGIFKSADPLQRAKAIVRAVENWNNPQIIAEVSTGLGQA
                     MDGIDVRQLDEKQLLSLRGW"
     gene            12905..13798
                     /locus_tag="PKW26_04400"
     CDS             12905..13798
                     /locus_tag="PKW26_04400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ92842.1"
                     /translation="MKLGQKRKKLKKIYLEITNNCNLACAFCAPTSRKKKYMSNEDFA
                     HILEKIEGRAEILYLHVMGEPLLHPQIDACINEAVKKGFKVHITSNGSLLSQVRDRLT
                     DTGLKRLNLSLQSLEQFPEKRRAEKTIEILEAVDFLLEKNKNLFVSLRLWTQDKKAFT
                     QEINAYLETYFSLPHLEIEKRLLEKNSFLIHPRLAIHAADSFDWPDTSQPFVGERGFC
                     MGLRDQAAILCDGSLVPCCLDRNGAINLGNLLEEDWDEIMQSKRASTFYEAFSERRVV
                     ETICKHCSYRLRFDKEIGKNI"
     gene            13898..14824
                     /locus_tag="PKW26_04405"
     CDS             13898..14824
                     /locus_tag="PKW26_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOQ92843.1"
                     /translation="MDIHKKAKPFLKWAGGKTQLLSDIELILPKNLTKTEFTYVEPFV
                     GSGAVLFWMLNNFPHIKKAVINDVNTDLIDTYKTIAHKPKELISILKLLESEFHSLEG
                     NEDNKKLYYYEKRFLFNSRTESKINQSALFIFLNRTCFNGLYRVNQKNEFNVPIGSYK
                     KPTICDSENIYAVNEALQKVEILNGDFDQTLKHVSPKSLFYLDPPYKPLSETSSFNSY
                     AKAEFNDSEQIRLRDFCVQLDQLGHIWILSNSDVKMTNKNNTFFDDLYVDFNIQRVEA
                     KRIINANPNKRGKLTELLITNQFSEGTYVKAI"
     gene            14808..15650
                     /locus_tag="PKW26_04410"
     CDS             14808..15650
                     /locus_tag="PKW26_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002677798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HOQ92844.1"
                     /translation="MLKPFEVFLSQLSETNATLDYFTDFNKVKGNVSKIELKLNQLNY
                     LIGKENLKEAVNEIYKENPTAFAVLDILIATRKSKKAKTFNKKGEIVLLDTYFTSPKL
                     IVEYIEETGLAEIFKNKNITNLVDYVFGVEVGLDTNSRKNRGGNNMSKTVAKQFTNAG
                     IFFKQEISSTDFKEIESLGADVKRFDFVIQTQKKTYLIETNYYNSGGSKLNETARGYS
                     DLALKINQYEKYEFVWITDGQGWLSAKNKLEEAYNSIPSVYNLVTLEDFIKKIQKEGV
                     VSIV"
     gene            15679..>15963
                     /locus_tag="PKW26_04415"
     CDS             15679..>15963
                     /locus_tag="PKW26_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOQ92845.1"
                     /translation="MLTPYFKSDDNNFYLLHGDTMKLLPRFEHKFDMVFADPPYFLSN
                     NGLSIQSGKIVSVNKGMWDKSQGLDYINNFNRQWLSLVRDKMKEDATIWIS"
BASE COUNT         4960 a         2900 c         3282 g         4721 t
ORIGIN      
        1 tcttttgtta ccttttttgg aagaaagcca tgcagttttt tgatactcac gcccatatcg
       61 ggttgattta tgatgatccc atcgaacagc ttcgcgttat acaggaagca aaacaagcac
      121 aagtgactcg tatagtaagc atttgcaata gcctacatga ctttacaaag gtatatggaa
      181 acctaaaagg cgtgcagggt atctatcatg ccgttggagt tgctccatcc gaggtaacaa
      241 atcctgggaa aaattggata gatacagttg aaaacgccct ctctttgcct aatgttgttg
      301 ctcttgggga aactggtctt gattattacc gaaagtttgg agataagcgc tcacaaatag
      361 agctttttat aacagaactt gagttggcag aaaaacacaa aaaacctgtt attattcaca
      421 atagagatgc aggtgctgat gttttagata ttttacgaga gcgcatacca agcgcaggtg
      481 ctgtgttaca ctgctattct gaaaacgctg agtatgctca aagggtttta gatttggact
      541 tgaacgtcta tttttctttt gcagggaatt taacctatag aaatgcaaaa aacctacacg
      601 aaacaattgt gatgctccca ctcgacagag ttatgataga gtccgaaagt ccttttatga
      661 taccggctga gtttcgactt ttaaaacgaa atatgccctc gtatacgcct tcgacagctc
      721 gatttatggc agatatgttg gatatagact ttgaagagct tgcagcacaa ctgtggaaaa
      781 actcatgtac tttttttaat ctacccgaat aaaatttgaa tgaatgcatt attatacaag
      841 cctgtttcta taggaagtgt acatattccc gggaatcttt ttcttgcacc tgttgccggc
      901 tattcagata gagcctttcg ttctatttgc atagctgaag gggcaaatct ttgttatacc
      961 gagatggttt cagcggaagc gctttggcgt ggttctgata agaccgaaat gcttttgctt
     1021 cgtggagaaa acgaggcgtt ttttgcaccg caaatttttg gtggcgaagt cgattccatg
     1081 aaaaaggcaa caaggattct tgtagaaaaa tacacaccct ccttaatcga tataaatgca
     1141 ggttgccctg taccaaaaat tatcaagtca aatgcaggct ctgcactcac acgagaccct
     1201 cgtcatttat atgctatcac aaaggcggtt gtcgatgagg caggctctat tcctgtaaca
     1261 ataaaaatac gatcaggctg ggatgcaaag aatcttaact ggaaagaaac gtctcaagca
     1321 gcctatgagg caggagcaca ggcaatctgc ttacatgcac gaacacgcgc tcaaggctac
     1381 gaaggttttg ctgactggca gattctcggt gaactcgttg cgtttatagg aaaaaagcta
     1441 ccagtttttg gctcaggaga cgtcttcagt cctgaggcag caaaagaaat gctagaaaaa
     1501 acaggctgtc atgcacttat gtttgcacgc ggtgctatgg gcaatccctt tatctttcgg
     1561 caaacgcgag atcttttact gtcaaatagc tacgaagcaa ccaggataga agacaaagtc
     1621 atgcgagcta tgcaagaact ggacctcctc atagtggata agggagaaga acttgcctgt
     1681 cgtgaaatgc gaaaacgctt ttctgcctat tcaaagggct tggaaggagg aggaaaaatg
     1741 cgcttggcga ttgtttctgc aacgaaaaag gctgattatg aggaaatttt taaaaatttc
     1801 ttgtaattat actctaaact tattacaatt agagcatggc tttattaaat tcaattgccc
     1861 agtttctcaa acgcttgttt cacaaagaaa aaaacgtgca ttctcaagcg cctcagctgc
     1921 gaaaaattga attggagcta aaaaatcacc ctgcacagat ttataaaaat gcttacatta
     1981 caccggcctt tgctcaggtg atacatattt tgcaaaccga aacagtaaag atgcataatt
     2041 ttcttttgga aaccctttac aacccaaatc gaaaaatagc catgcgttat agtacgagct
     2101 taatagaaac aggttttaca caagaacaac gagacttgct agaatcgctt tcatacgaga
     2161 atcgtaaaaa agaatatctc ggaaaagctc tcggcgttca tgcggaaata aaaacaaatc
     2221 aagaaaaggc tattgcaaga gctctttctt ttttcgatgc agatgagttt aaaaaaatag
     2281 agcaagtgat acgtaacctc gatcatcttt ttgatgtttg tcagtttaac tatgtttcgt
     2341 ttttgacaca atttgaccag ctttatagcg agagaaaaga tacagcacct cgcttttctc
     2401 ctcttttgtt agctgatgct gaaacccatc ttgtagattt gtattattta gctgcgggat
     2461 ttactctgag tcttgcagaa gcaagagccc ttgaggcgct ctgttttgtg cacaatggct
     2521 atgaacaagc aaatcaaaaa tgttttttga aagaagtaaa aacaatacat gcggcattta
     2581 aaaatatttt gaacgccgat accttacaaa aaatgatttt tattattaag ggtaaaacgg
     2641 acgttctctt gcaagttgct acttataatc gcaatgttat agaaccttat aaagaggaaa
     2701 tgaaaaaaaa atttgattct gaatcaaaac gcattgctgt tgaattgcag gatgaaaaaa
     2761 acactgagga tattaaaact ttatttgaca atcgaaatct acttcccttg caaggttata
     2821 cgcaagaatt gagtcatttt ttacagtcaa attccgtaga aggttttttg catattgtgc
     2881 cattatccat aattaatact tttatgcgta tgtttttaac cgatagaatc gtaagttatt
     2941 tgaataggat tgttgtagag ggttttttta caaatccttc atttaaatct gatttttcat
     3001 ccgctgtttt tgcctgctca gagatagata caaaactaag aagtttcgaa aataccttta
     3061 ctaaaggcca gactaacgac cttgctgtct tacgtagtaa tgctttggaa ggacaacaaa
     3121 atgtcgattt gctgaaaaaa gtaagttttc agattgatac aataaaccat gatgcacagc
     3181 ggcttatgca agaaatcatg aacgatatca acagtctcgc tcatattatt gaagttttgc
     3241 tttcagaagc taaaaaatca aacccttcag atatttctaa tattaaatta ctcgtttctt
     3301 cagtacgaaa taaggatgcc gcagaggaat tggaaaataa tttttcaaaa tggatgtttt
     3361 ttattgatat tatgcagaat tatgttataa tcaaagataa agataggaag aagttgggaa
     3421 catgacgcaa acaaataaac caaagaaaaa actttctctt caggagaagg ttgttctgta
     3481 tgaaaaacaa ctctacgatt atagacaatt gctggaaatg tcgaagagtt tgtcgtctgt
     3541 gttagaaata gacactctga ttgaatcaat tctctacgtt tgtatgtgcc aaatgcgggt
     3601 gcttggcaca ggggttttta ttctaaagtc ttttgatgca gatttttttg ccttagattc
     3661 taactataat gggcttgagc tcgtcgacag tgtttcgtat ctcataccga caacgcatcc
     3721 gcttatacct tttttggcag aaaataatag aacctatacc tacgaagagc tggagagctg
     3781 ttttaaagat acttctattc ttgaacaaat ttcttcttta aagccttctc ttattattcc
     3841 cttaaagcaa aaaaatcact taaacggcat tttgcttttg ggtgagcgca tagatgttgg
     3901 tgaaggtgtt gcgtactctg actatgagcg agagcaaatg ctcgacattg cgtcgatttc
     3961 tgctatcgca atcaataatg cgagtttaat tgaaatgaca actaccgaca tgatgacacg
     4021 gttaaaacta aaacactatt tttatactat tctcgcagag aaacttgact tttcttttga
     4081 aaatagactt ccgcttggtg ttctcatgct tgatatagat cattttaaaa aatttaacga
     4141 tacctatggt cacgcttgtg gggattatgt tttacagcaa gttgcaaaaa aaatctttga
     4201 aggtatacgt gggcaagata tggcagggcg ctatggcggg gaagagtttg tcgttatgtt
     4261 gtacaataca gaaaaagatg cagctatgct tgtcgcagaa agaattcgaa aagatataga
     4321 aaaagctgaa ttacattatg agggacagga cctttctgta actatttcta ttggtgttgc
     4381 tttttgtcct cctgaaacgt caatgacagc aaaagaaata gttgagtttg cagatcaagc
     4441 cttgtatgaa tctaaggcaa atggccgaaa tcgcgtgact cttgcacaaa catacaaaga
     4501 caatgctttt gctggagaaa aatgaaaacg aatacaatta taaaaaagcc atttcgttat
     4561 acctttaaaa attttgcttt ttatctgata gccatcaatg ttattgtgta tttttttaca
     4621 tcaatgacct atcgccttta taggagtgac tttctttatt cctatctttc tttaaacgta
     4681 ataaacgttg ttattcacaa aatgctttgg cagtttataa catacatgtt tgtccatggt
     4741 agtgtatcgc atttgttttt taatatgctt gcgctttttt tcttcggaaa catgttagaa
     4801 cgtgcaatgg gttctaagga gttcttgctc ttctatctat taacgggctt tttaagcggg
     4861 tttctttctt ttatcattta ttatttttca ggggggcatc gtgtttttct catgggtgcc
     4921 tctggcgcaa tttatgctgt attgcttgct tatgcggtgt attttcctag aaatcgtatt
     4981 tttatctggg gtatcgtacc cgttcctgca cctgttttag ttgtcgttta tgctgggata
     5041 gagtttgcaa gtcaatttct gagcttgcgt aaaggcgtgg cgcatattac acatcttgca
     5101 ggttttgcct tcgcatggct ttatttgcaa gtgcgcatag gggtaaatcc tttaaaggta
     5161 tggagaaatg cctttcacca ctaataccat aaaaaaaaat cttttacttt gtgtttttct
     5221 tgtcttggtt ttttgctgtt tttctcaagc agctttaatg gaaacagaaa gcgctgaaaa
     5281 tgtcttgcgt ttttccctct ggtccttgct tgaagatgag ccttccttgg aaaaagaggc
     5341 gctcgaaaac ttttacgaca agtcggtgca ggagctaaaa atacttgctc cttttttgct
     5401 tgagggcatg atctacgggt ggagtttttc ctatgtgcca tccgacattg cacggcaagt
     5461 tgccgaagaa cttgagattt tgcctttggt tcgtattgct ttgccagatg aacggctttc
     5521 ttttttggag ccacgaattg aagatgagcg tttttatgta tggcttgagt atcggagaac
     5581 gaattcgatg atggcatata aaaaagcttg ggattctgtg gtctatccaa aggcgaaggg
     5641 catagggcag gcttctttgt tgatgggcac agagggaatt ttggaagcct atgaacaagc
     5701 gctgaaaaat gcaattcgtg gttttgtaca aaaacaagaa aaaaacaaac cgaggcgtat
     5761 ttcggggcgg gtcttgcttg tgggtaaacc gatgcttggt attcaagccg gtcgttatac
     5821 tgcagccctt gatttttttg ttcatgttat taaaatagaa cggtatactg aattttaaat
     5881 aaaactgagg cttattatgg atatccaaaa cattgtaaaa aacttacatc ccttagaaat
     5941 aaaggtgatt aaatctttca aacctggaca agagctcaca agtgaacttt tgcaaaaaga
     6001 actggactat aaagaagggc atgcaaatca agctttttcc tggttgagtg gaaaggagct
     6061 tgtttcggtt gtaagaaaag aagcctttgt tttttatgaa ataaccgaac ttggaaaaag
     6121 ctgggctgaa aatggtacac cagaagaaaa ggttgtcgca tttttgaagt cttcagggag
     6181 tcatagtttg cctgagatag caaaggcgct ttctatagaa aataaagacg tcggtagtgc
     6241 ctttggtctt ctttcaaaag aaggcgtgct caaaatggat gcgacaaagc aagcgagccg
     6301 tacaggaaag gatagctctc ctcgtatgga gatggtaaag aagcttttag aaaaagctct
     6361 tgcttctcct gacggcatac tcgaccatgc acatttagaa aaagatgcgc aggaggttat
     6421 ggcaggactc gcaaaaaaac gtggtgctag tgatgcagcc tttagaataa tcgaaaaaga
     6481 aattgtcttt tatagtttaa aagatactgt tgaggagctt ctttctgcct tagatgcggc
     6541 aggcataagc ggtaatgaaa taggttcttt aacgtccaaa atgctttcta caggtgagtg
     6601 gaaaaatgca aacttccgag gctataatat ttctgtgcca cctgctcggc ttattccggg
     6661 gcgaacaaac ccctatgtaa actttttgga aagcgtaaaa gacaagcttt cttccttggg
     6721 ctttgaagaa tttgacggac ctctagttga aacagagttt tggaactccg atgcgctttt
     6781 tatgccacag ttccatgctg cccgagatat tcacgatgcc tattctctta aaaatcctac
     6841 tcatgcaaaa agtatagaag agcctttttt aacgaatgtt gcacaagccc atactgacgg
     6901 cggtaataca ggaagccgag gttggaatta tgcctttgac agagatttta cccgccgcct
     6961 tatcttgcga agtcagggaa ccgttctttc tgcccatcag cttgcaaagg caaaaatccc
     7021 aggaaagtat tttggtatag cacgctgttt tcgctatgac aaggtagatg caacccacct
     7081 ttcggacttt tatcaaacag aaggtattgt ccttggaaat gaggtaaacc taaaaacact
     7141 tttgggcttt ttggaaatgt ttgcgattga aattgccgga gcaactgatg taaaatatgt
     7201 tccaggttac tttcccttta ctgaaccttc tatagaggtg catataaaac atcccgtgct
     7261 cggctggttt gaacttggtg gttctggtat ctttagacct gaggttacaa aacccatggg
     7321 tatcgatgtg ccagtgcttg catggggaat tggcattgac agaatggcgc tcatggcatt
     7381 gggcttaaac gacttgcgag aattatttag taacgatata gaacaagttc gcctgcgaag
     7441 ataagctagg ttcataaaat gacgaagcga cagtggcagg cctttgaagc atttcgagaa
     7501 aactttagaa aaaaatgtac ggagtggaat gcaaaagcgc tctcccttgg ttttcctcaa
     7561 gcacaaaagg cccttgccat aaaagatgct gttccagaat atcccataga aaattccgta
     7621 gtctataaca caaaacttga tacagttacg aagcaaagcg atataaaact catccttgta
     7681 agcgataatc cggggaagga tgagcaacgt gaaaaaaaca aagcctatct cgtggggcag
     7741 gctggaaagc ttgccgaagg tttttttagg aaaaatccag agctgggaat tcattttagc
     7801 gaaaatctac ttatcttaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 aaatagaatt acttattttt ctacctagtt ttgaaaccta ttcaccgatg agcggtgttt
     7981 ttttgccatg ttcatcaaca gcgacaaggg taacgcggtt agagaaaata tgtttttctg
     8041 ggtttcctgc aaggtcttca gagtaaacat cgaccgaata gacaagggaa gttgtgcctc
     8101 gttttatttt ttctagatgg aagcgtaaaa tagcaccgtt gggaaccatg tgatgaaact
     8161 ctatattttc cattgctcgg gtaacgaggt tgtttttttt atactctcgt ttagcggcaa
     8221 gccaagaaaa ttcatctacc catttgagta gttgtccgcc aaataatttc ccataatgat
     8281 ttaagtgctc cgagcgcacc atggtaaatg tatccatatc gataatctcc ttatcttgta
     8341 cttgcaaata aaaagggcgc cttatttgca agcttctacc atggtacgag catagcatta
     8401 tctagaaaaa tcttctagaa gaattcatct cctgccccgt atttttcaat gataaaatcg
     8461 atgtctttgt ctccacgtcc agagaggttt acgagaatcg atttttgtct atttttttgt
     8521 gcatggcgca ttgcaaaggc aacggcgtgt gcactttcaa gtgcaggaat aatgccttcg
     8581 tagcgacaga gtttgtaaaa agcttcgagg gtttctttgt catttgcaat ttcgtaatga
     8641 acgcgttttt tatccttgag catacaatgt tctggtccac ttccaggata atctaaacct
     8701 gatgcaatag aatacacagg tgccggctcc cctttttcgt cttgtaaaag ataacttttg
     8761 aaaccatgta agatgcctgg ttttccgtat gacatacttg cggcatggtc gcccatcttg
     8821 agagagcgtc cgccgggttc aacgccaatg agcgaaactt ctgcatcgtc gaggaagcct
     8881 gaaaaaatac ccattgcgtt agagccgccg ccaacacagg ctgcgactgc atcgggtagc
     8941 tcgccagtca tttcgaggaa ttgctcgcgt gcttcaacgc cgacaacgtg ttggaagtca
     9001 cgtaccatca tagggaaggg gtggggacca acgacagaac caatgcagta aatggtattg
     9061 attgggtctt tgaggtaggc ttcaaaggct gagtcaaccg cttcttttag ggatttaagc
     9121 ccgtggctga caggtacaac cgtggcaccg agcaatttca tacgcacaac attgggatac
     9181 tctttggcaa tatctacttc acccatgtga atttcgcatt cgaggccaaa ataagcggcg
     9241 gcggtggcaa gggcaactcc gtgctgaccg gctcccgttt ctgctatgag ttttttcttg
     9301 ccaagatatt ttgcaaggag accttcgccc atacagtggt tgagcttgtg tgcgcctgtg
     9361 tggttgagat cttctctttt gagataaatt cttcctccgt agatttcgct caagcgcttg
     9421 caaaagtaaa cgggggtcgg tcggccttga taatgcttgc gaatcgatcg aagctcggag
     9481 ataaattcgt ggcttttact tatggaaaaa taggcctcgg taatcttatc catttcgctt
     9541 tgtagttctg gtggaacaaa ggatccaccg tattcgccaa aatagccatc ttcagttggg
     9601 tgttgtttta agtaactcat tttcttttcc ttatgcagtg tatttagaaa tcttagcttg
     9661 tataaaaatc cgccttgttt ctattgacag taacaatata gcactagtct atcgcattgt
     9721 caagttgttt tttgtctttt ttaagtgaaa aatctgggat taaaactgtg aaacaaaacg
     9781 aaggcttgct ttaggctcag cgtgttcaaa gtcccaaccg acataggctt caaggttaaa
     9841 agaagaaagc ccagcgagcg atatttcttg ttttgaaaaa agcccgacaa tgaacaaggg
     9901 gtggttttga aagctaaaat cgtagagggc atcggtaaaa agtccgagag agagcgcttc
     9961 gagcttgaag gcttccatgg cggtaaagcc agtgtttttt gtgtaccaat aaagatttaa
    10021 ttgacttaag gcgatagagt ttttattggc ttgtgaagaa taatcataga tagctcgaaa
    10081 gccgtcgttg taaaaaagca ctgcctcttc tgcgaagggg gtgtagcgca tcagttgtgt
    10141 gagagaaaac ccaagcttgg aaaatggatg gaccttgcta aaaaaagaga gcgtgaggcc
    10201 gagattattt ttggtgaatt ctggagaaag ctttgcggtg ttgtaaaaaa ctgaaggctc
    10261 gatggaaaac tgatacggct tgtcttttgt attgaataga gaaaaaccga gctgagaaaa
    10321 taaagctttt tctctaaaat ccgcgtcgct taaaagttct gcggtaaaaa gcggtttgaa
    10381 aaaaacttct tttagtggag aaaaaatcca tgaaagacca agtccgacat aatggctcgc
    10441 ttggtttaga tcgccgtagg gacttctgta gttgtatgaa agttttgttt caaagccaga
    10501 agaaaagtga agggagctaa aaaaatcgcc gccaaaggaa ttgaaaatat ttgtatgagc
    10561 gccagctcgt gtaaaaaaag ggccaaagtc aaaaaaagca taggcgccga ttgttggctc
    10621 gggtgcaaga agaaattctg aaaagtcaat aaccgctacg ttcggccgta ttttaagacc
    10681 aagatagggg agattacttg tcggtttacg ccccctttgt atttcctcga taaaaagcgc
    10741 tgcagatttt tgtctttcgg agcgcaatga ttgaaagccg acatcttcat cgggcggaaa
    10801 attcccatga acaatttcag gttttgcatt gagaaaagcc ttagtcgaca tttctcccct
    10861 gccaataatt tcatgcacct tggtaaaatc cataaatgaa aattgttcaa catcgtttcg
    10921 gataagatgc ggttcaaaat ccaatatatc ttggaccagt tgtcgttctt tgccaataga
    10981 aaaagttctg tttaaaacag ttacgggatt gttgagattg agctttgtat catacagcgc
    11041 agttgaaata ataatattgt ctgtaaaccg ttttgcgatt ttcaaactgc caaggtcaat
    11101 agaaccactg tcgactaaag ataggttttg ccccgactcg agtagatagg ggacgggctc
    11161 catcataaaa gacatagcaa aggaggcagc cataattttt gttatttcac cttctgcaaa
    11221 aagcacctgt cgttttgtat acaggtcttc tgttggcacg atgatgggta tgaatgagtc
    11281 ttttatatca aagtttttgt ttgggaaaag acgcgataag ctttcgagaa aatatcgcga
    11341 agataagatg ccgccgcgaa gcggaaagac aatatcaaaa aaactattga ggtcaacctg
    11401 cgttacgatg gcttctatgt cgtcgggcga aaagcctgag gcatagagca agccgataat
    11461 agctcccatg gaatttgcaa caataaaatc tggcttaata tttgcttttt caagttctcg
    11521 taagacaccg atgtgtgcat aggcgcgggc tgaaccgccg cttaaaacaa gggcaagagg
    11581 ttctctctct tctcgtagct tatcaatttt ttggctaaaa gcttcttcac caataaaaag
    11641 agcgctgctt tgaaaccata cagaatcaac ttcgttttgt tcagcaaaga gatgtgttaa
    11701 ctgaaagaag ataaaaaata aaaagagtga ttttaaaaca tattttttta tctgcatgaa
    11761 atttctacac cttatctaaa aaactatcta aagaaaacgt ctaaagaaaa cgtctaaaga
    11821 aaatagctaa aaaaaagccg tctgctttta acaaacggct ttctagatta ccagcctcga
    11881 agggagagta attgtttttc gtcgagctgg cgaacatcga ttccgtccat agcctgacca
    11941 agaccggtgc ttacctctgc aatgatttgt ggattgttcc agttttcaac tgcacgtaca
    12001 atcgcctttg ctctttgaag tgggtcagca cttttgaaaa tgccagaacc tacgaaaaca
    12061 ccttcgcttc cgagttccat catgagggct gcatcagctg gcgttgcgac tccaccggca
    12121 gaaaaatttg ctacaggaag ttttcccttc tttgcaacaa actcaacgag gtgatagggc
    12181 gctgcaatat ctcgagcata taccatgagt tcatcataag ccatagattt gagcttgcgc
    12241 atctcggctt gcacagttct catatgacga accgcttcga cgacattgcc cgttccagcc
    12301 tcacccttgg tgcgaatcat agcagcccct tcgccaatgc gtcgaagagc ttcacctaag
    12361 tttcggcaac cacagacaaa gggaacattg aaaacttttt tgtcaatgtg aaattggtca
    12421 tcagcgggtg ttagaacctc actttcgtca atgaagtcga cgccaatcgc ttcaagaacg
    12481 cgcgcttcgg ttatatgccc aattcgagcc tttgccataa caggaatttt taccgctttt
    12541 tttatttcga taatcatacc gggatcactc atgcgagcaa caccaccctg cactctaata
    12601 tctgatggca ctctttcgag agccataaca gcgacagccc cagcttcttg agcaattttt
    12661 gcttcttcaa cattggtaac gtccattata acgccaccct tgaaactatc tgcgagctta
    12721 caaaattggt taaatttctt atctctttcg cttgccatat atactcctac aagactttca
    12781 aaaataattt tgaaaagtct tattataaaa atattgtgta cacattattg ttattttatt
    12841 aagctaaata ttacttccat actactatac tcaaaaacga gggcaatgta tagtaagtct
    12901 taaaatgaag ttagggcaaa aacgaaaaaa acttaaaaaa atatatctcg agattacaaa
    12961 taactgcaac ttagcctgtg cattttgcgc gcctacaagt cgcaaaaaaa aatacatgtc
    13021 gaacgaagat tttgctcata ttctagaaaa aattgaagga agggcagaaa ttctctatct
    13081 ccatgtgatg ggcgagccgc ttttgcatcc acagattgat gcatgtataa acgaagcggt
    13141 gaaaaaaggc tttaaggtac atatcaccag caatggaagc cttctttctc aagtgcgaga
    13201 tagattgaca gatacaggac tgaagcgcct gaacctttca cttcaaagcc ttgagcaatt
    13261 ccccgaaaaa aggcgtgcag aaaaaaccat agaaatctta gaggcagtag attttcttct
    13321 agaaaaaaat aaaaatctct ttgtgagcct tcgcctttgg acacaggata aaaaagcatt
    13381 tacccaagaa ataaatgcct atcttgaaac ctatttttca cttccgcact tggaaattga
    13441 aaaaagactt ttggaaaaaa actccttctt gattcacccg cgccttgcca ttcatgcagc
    13501 agacagcttt gactggccag atacttccca gccctttgtt ggagagcgag gtttttgcat
    13561 gggtttacga gaccaagcag ccatcctctg cgacggcagc cttgtaccct gctgcctcga
    13621 cagaaacgga gcaattaatc ttgggaacct cttggaggaa gactgggatg aaataatgca
    13681 gtcgaaaaga gcaagcactt tttatgaagc cttctccgag cgccgtgtcg tggagacaat
    13741 ctgtaaacat tgctcgtatc gattacgctt tgacaaagag ataggaaaaa acatttaaca
    13801 caaaaaaatt ctcctttgat cttgaccgat tttgtactat aatgggagta tgtagaagag
    13861 gggccgcaga gttttgaaga gccaatagga gacatggatg gatatacata aaaaagctaa
    13921 accctttcta aagtgggctg gtggaaaaac acagcttctc agtgatattg agttgatttt
    13981 gccaaaaaat cttacaaaga cagagtttac ttatgtggag ccatttgtag gtagtggggc
    14041 ggttttattt tggatgttaa ataattttcc acatataaaa aaagcagtca taaacgatgt
    14101 taatacagat ttgatcgata cttacaaaac aattgcacat aagcccaaag aacttatttc
    14161 tattcttaaa ctattagaaa gtgaatttca ttcattggaa ggaaatgaag acaataaaaa
    14221 actctattac tatgagaagc gctttttatt caattccaga acagaaagca aaataaacca
    14281 atcggcactt ttcatttttc taaatagaac ttgttttaat ggtttatatc gggtgaacca
    14341 aaaaaatgaa tttaatgttc caataggaag ctataaaaaa ccgactattt gcgatagtga
    14401 gaatatttat gctgtaaatg aagccttaca gaaggttgaa attttaaacg gggattttga
    14461 tcaaacacta aagcatgtta gtccaaaatc attgttttat cttgacccgc catataaacc
    14521 tttaagtgaa acttccagct ttaattccta tgctaaagct gaatttaatg attctgagca
    14581 aattcgctta cgagatttct gtgtgcaatt agaccagcta gggcatatat ggatcttaag
    14641 caattcagat gtaaaaatga caaataaaaa caatactttt tttgacgatt tgtatgtaga
    14701 tttcaatatt caaagagttg aagcaaaaag aattattaat gctaatccaa ataaaagagg
    14761 aaagctaaca gaattactaa ttacaaacca attcagtgag ggtacctatg ttaaagccat
    14821 ttgaagtttt tttatctcaa ctttctgaga caaatgcaac tctagactac tttacggatt
    14881 ttaacaaagt aaagggaaat gtcagtaaga ttgaactcaa attaaatcaa ttaaattatt
    14941 taataggaaa agaaaactta aaagaagctg taaatgaaat atataaagaa aatccaacgg
    15001 cttttgcagt cttagatatt ttaatagcta ctcgaaaaag caaaaaagca aaaacattta
    15061 acaaaaaagg tgaaattgtt ttacttgata cttattttac ttctccaaaa ttgattgttg
    15121 aatatattga agaaacagga ttagcagaaa ttttcaaaaa taaaaatatt acaaacctag
    15181 tagattatgt tttcggcgtt gaagttggtt tagacactaa ttcacgaaaa aatagaggtg
    15241 gaaataatat gtcaaaaaca gttgcaaaac aatttactaa tgcaggtatc tttttcaaac
    15301 aagaaataag ttctactgat tttaaagaaa tcgagagttt aggagcagat gttaaaaggt
    15361 ttgattttgt tattcaaact caaaagaaaa cataccttat tgaaacgaat tattataata
    15421 gcggtgggtc aaaactgaac gaaactgcaa gaggttattc agatcttgct cttaaaataa
    15481 atcaatacga aaaatatgaa tttgtttgga ttactgatgg tcaaggttgg ctttcagcaa
    15541 aaaataaatt agaagaagcg tataatagta taccgagtgt gtacaattta gtaacgcttg
    15601 aagattttat caaaaaaatt caaaaagaag gtgtagtatc aatagtttga tagatattat
    15661 atttgaaggt gtttgaaaat gttaactcct tatttcaaat cagacgacaa taatttttat
    15721 cttcttcatg gcgataccat gaaactttta ccccgatttg aacataagtt tgatatggtt
    15781 tttgctgacc ctccttattt tttatctaat aatggtttgt ctattcaaag tggaaaaata
    15841 gtaagcgtga acaaaggaat gtgggataaa tcgcaaggtt tggattatat caataatttt
    15901 aaccgccaat ggctttcttt agtgagagat aaaatgaaag aagatgctac tatttggata
    15961 agc
//
LOCUS       DAOSSW010000035        15904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_3788_length_15904_cov_6.493217, whole genome shotgun sequence.
ACCESSION   DAOSSW010000035 DAOSSW010000000
VERSION     DAOSSW010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 15904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15904
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3788_length_15904_cov_6.493217"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PKW26_04420"
     CDS             complement(<1..203)
                     /locus_tag="PKW26_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92846.1"
                     /translation="MSKKIEAYKDALVFALEKELGIIISYEKMSPSVLNSLKISNITV
                     FDTKDDSALVQIPSIRVYFSLLS"
     gene            370..993
                     /gene="tmk"
                     /locus_tag="PKW26_04425"
     CDS             370..993
                     /gene="tmk"
                     /locus_tag="PKW26_04425"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOQ92847.1"
                     /translation="MILDNFIVFEGIDGSGTSTQLSLIKERHPDKKLFFTAEPTNSPI
                     GLFIRSILKGELEIAPSTMAYLFASDRNEHLYGREGILAQKENGFIVFSDRYLFSSLA
                     YQNPICPEGLPEKLNENFPLPEYLFYFDIEPEESLSRVHMRGETEIYETLSIQRQTQA
                     EYKKILSAYKEKNIPGMHIVEIDARKPIEVVYQKIMDTLVNLPIYTK"
     gene            999..1619
                     /locus_tag="PKW26_04430"
     CDS             999..1619
                     /locus_tag="PKW26_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92848.1"
                     /translation="MHFFGKKRIFLIAILVFLFILPSFSYFVTYKEQYYRLFHVHYQQ
                     YPDDIMENIYWLEKAVAADFSNPKYALTKIDDEKDWEKYRSVFMMHLNLKLIEQHLRL
                     GGKYDKGKVYFYDAPFREALLFELERAESCYQAGLYYWREAKLWAEKASEKKFYFLNL
                     SGIQNWEDERERIINGKLNYEKIITRELKRIAENKAYLLAMDENTY"
     gene            1629..2729
                     /locus_tag="PKW26_04435"
     CDS             1629..2729
                     /locus_tag="PKW26_04435"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HOQ92849.1"
                     /translation="MKHETFDIHYPPIQLGCEKTSILYFETIQELTKKLKENIDNALI
                     VTDDVVFNIPGLKDFFAGIPTETCLVLPAGEKYKTIETVLSILERAMKLSLTRKSLFV
                     GIGGGVITDLLAFAASIFKRGARLELVPTTLLAMVDAAIGGKTGCDFKGYKNSLGTFY
                     PASKLYISPEFIQSQSEDEFRSGLAEVFKTGLLFSEKLVLLMQDEKDALLNRDQRLLM
                     NIIKRCAQAKAQVVEKDLTEKNIRMWLNLGHTFAHALETVLGLGTISHGEAVAWGISR
                     AIALSESIGLCAAEYREEVFSMLKAYNWSTDKIHPKCIELKIENCSEKLIEAMKQDKK
                     NIDSNISFVLQRGINDSIIEKISEDKIRSILS"
     gene            2786..2869
                     /locus_tag="PKW26_04440"
     tRNA            2786..2869
                     /locus_tag="PKW26_04440"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2820..2822,aa:Leu,seq:gag)
     gene            complement(2905..4653)
                     /locus_tag="PKW26_04445"
     CDS             complement(2905..4653)
                     /locus_tag="PKW26_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOQ92850.1"
                     /translation="MKITKKNLEKQMMASGFLPNMLSGLILMPYIFIYSGVSTNLIPK
                     AMILGLVVMLSVQQLVAPFTNKFITLSVSKRLHIFQNKETDIQDRTLLLSDLLVLPKY
                     IAIQVGLVFYLGCIIWVFLCFALVDFSLAESVFALIAALLGSIISSLFTFNMAENLCS
                     AYAKPIVKKGVDDKTVFSKQFFGSRLKITYVFYNIIPLVFSSLIFFILLWFGYLSKNP
                     LLNAYDEFGSMFRIALLFFLNVLFCIVLNRIYFSHMYKINANMTSAMLSIVSREEQNS
                     DFLDTDLSNELSFNAYLINKIITIFNVLFQNTGAIASSITKSTQDLVIISKQTESISL
                     EQSASVKEIVATMEDSDKLSRNILTRIGDVALVANKTKQNVTHGVKTLATNVEKMREI
                     NDANKDTITGIKSLSDKIEGVWDIVNIINTITDQTKIIAFNAELEAASAGEAGKSFNI
                     VATEIRRLADLTTNSTREIKQRITEIQHSSDNLIITSEGGTEKIQEGITLSANLEENF
                     NYIKSSAEITAESSSDIRDIIEQQTFAFEQIVVTLRQIAAGVESFSFSTQAITAASEE
                     LKKIAETLETSVLKGN"
     gene            complement(4653..6104)
                     /locus_tag="PKW26_04450"
     CDS             complement(4653..6104)
                     /locus_tag="PKW26_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOQ92851.1"
                     /translation="MFVELLRCPRIIMYYVFILFGSASLLFILLTYFFIGISLVKLIF
                     LFTVCVFASYVAGVIALFTSEIVCHKYAIPIFDSGIDLGELESKKYYGISLKIQFLLF
                     TIIPALFVSVLTFAYFLIQKDILLEQNIFSLGRTVLFFLTNTVILISLFLAFRNNSIL
                     EIAKVQYLLEWLSKTDFKTEKKIPVALSNEISYISFLLKNTIHRFPRILDFYKRISSE
                     ISELVFSLKKYQAEQEPILETNHRVIGNINEEVKKINQSSDLFFAELDSFFDTHDQIK
                     IIIDEAYALISQSKDTALQNTNKNLSSTRELKSLENQLTGISEITSLLTDIADQIKII
                     AFNAELEAVKAGASGKNFLIIATEIRRLADYTMDSIIETQEQISLVHTTTEKLISHFQ
                     DSLEITKKSKTFLEKNEMLLDEMKEKAEINVIDIPDVQRYKIKEGQIHNAILSLTEQL
                     KHLLDEASLHAYDMSENIECFERLADRVAKGEV"
     gene            complement(6411..8243)
                     /locus_tag="PKW26_04455"
     CDS             complement(6411..8243)
                     /locus_tag="PKW26_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOQ92852.1"
                     /translation="MAKNKIVVCEYSKIVQNVIKSLLGKIKEEIVFFDNAYDALFFIQ
                     SNPVVLLISSIYLEPFTGFHLAHLIKAEPKTEKTKVLLYYSEETKLKSFYAEKTHADA
                     FLFFTANNTAELYEEISLLLEETETSETNLSEVSEKEVLVSALKATEKNFFNYIFLES
                     LFEMNLYMENTASLLENLLYLIAKIFEVEIFSFYIFHEAQVQKHSLISNSLAKTEEND
                     FYKVSKLHFESYVPDNFDISLLEYNEEKLYRFEKKTKTKQNAISSYEHFVLYANSFVG
                     TFHIGTVEFNYFTEQELKLLHFFCEKIGFLIEMSLRFTSVIKKEMRMKKTFSRFVPEE
                     IIDNILGHEGEAKQLVGEKRMVAVVIVDIRDFTAISEINTPENVVSFLNLYFTHMVEI
                     IKKNGGIVDKFMGDSILALFGATTSHEDNAQRAVKASLEMQEVIHTIDTSLLIFPENH
                     ELKIGIGIHQGNVVVGNIGCQEKIDYTVIGDSVNLASRLESLTKQYGAPIIISNAIKE
                     ELDQSFFTRHLDTVRVKGKSIPVSIYLVMQKKDAYSAEFQETIAKAMELYILGAWNLA
                     YNYFTKALDIMPQDKATMVLQNRCKSFASQPPLKWDGAITLLSK"
     gene            complement(8230..>9909)
                     /locus_tag="PKW26_04460"
     CDS             complement(8230..>9909)
                     /locus_tag="PKW26_04460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ92853.1"
                     /translation="KSIFDLPELLPSDIKKKILDIQTIEMSTERIDEIIKGFENIVLQ
                     QNRLKKYLDDITVELENNSSSFLKKLKKEFSDDLEHLENSMANLQKSIYEFRLISLST
                     ILEPLQKKIEAEALEQNKIIAFHFPEIDLFLDKDILDLVSEILYQLLKNVIDHAYETK
                     EIRLQKGKEERLQVFLGIYKHAEHITLELSDDGCGIDYEAVRFKALDLYPDKTAIILE
                     MTEKELSSFLFMPGLSLAQKSNQSPYRGDGLDIVRRNADKVKAKIFLTTEKDLGTEFS
                     LSIPTSLATLKGVFIKAGLFTFLIPVHYISEIYNCPKNDLLYLQNNTMFRLRDEVLPV
                     YSLNALIGEVVDTKEKGTQVILIVEYLEKKIGININELLHTVSVIIKPLPSILKKYHI
                     FQGLVLDENHRFVPILDIPELIERLELLQTYSLIKAEAKNIRDVKKILIIDHSTITRE
                     IQKNILEAEGFVVDTTEDGIEALSIVKKRKYDIIVTDIKMPRMNGYVFIENLKRIRGY
                     EKTPILVISSLEGRAVEEKTMEMGVSGFLFKSHFERRKMLSIVKGLINGEE"
     assembly_gap    9910..10009
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10094..11971)
                     /gene="cheB"
                     /locus_tag="PKW26_04465"
     CDS             complement(10094..11971)
                     /gene="cheB"
                     /locus_tag="PKW26_04465"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HOQ92854.1"
                     /translation="MTRVLLVDDSAIIRNMLSKSLQKNSLISVVGEAGNGLKAVELAK
                     QLKPDVIIMDVSMPLIDGIEATKRIMEKTPCAIVIFTSEDSFDLAYKALEFGAVEIIQ
                     KPDLSILTSSFYREFFDKIHAIAEANTGFYNRPLIQTQEKCFDSSIQGEARSIGCEKL
                     VYEIVGIASSTGGPLAIQKLLQELGPDFPLPILIVQHIETNFDTHFVSWLSQTSPLPV
                     HLAQHNQKIEKGHVYVAPANYHMVVVGSDFNKDFFISLNKEAEKHFLRPAADPLFFSL
                     AKLFANRCISIVLTGMGSDGAEGSLQLKEVGAYTIAESKESCVVFGMPKAAIDKGAIK
                     EILPLSAIPKTLLALVNELTTAQIDSVLQLIYAHCGMSINGSHVEYLKRYLKKRLALR
                     AFSFNLLYADLLKKKEEFELLINSITINETYFFREEKHFFYLRDIFLPQKKHTSLSIW
                     SAACSSGEEAYSLSILCKSLGIDAEVYASDINTFSLEALQKGNYSPSSLREDGSAFHT
                     LLEPYLTYGQKNFSLSKEILVTVQSFPFNLFRFDGCKDCLSDKKFDVIFLRNVFIYFS
                     DETKQACLRFMEGKLKPEGLLFVSTNEIASIQIAKESSLKKYKESNVFFFRKEGGITC
                     S"
     gene            complement(11987..12526)
                     /locus_tag="PKW26_04470"
     CDS             complement(11987..12526)
                     /locus_tag="PKW26_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOQ92855.1"
                     /translation="MGKEKKKKEVVEKRVIDLQEQKTLEDDVEIIDLEKIDAEEAQEV
                     FLIVKIAHTLYAFQSASVKEILYNPRIDPLPFMPPYIAGLINRHGEPFVLFNPHVLLE
                     NENTVKNIVIIIDSEEKFGIQVSGVETFFLGKISELTYDSMFRSSFFSFSILFNEHEI
                     SVINPFSFLELLNKEIKNT"
     gene            complement(12621..14369)
                     /gene="lepB"
                     /locus_tag="PKW26_04475"
     CDS             complement(12621..14369)
                     /gene="lepB"
                     /locus_tag="PKW26_04475"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOQ92856.1"
                     /translation="MKKHILPLQVLVIALSFCFTLLNLSFYADISALAFLPSLIFSLL
                     LAYLGLVLFSQRKSLPLSVIRKLYEYTPFVLLLTFILRRAGNNDTSYALDLIAVLVWV
                     AVTIFSNVFMYLSNPKRFYINNPDFTEPEIKLNKKGKKKISVIGEAISWIDAFVQAAL
                     IVTLVNIFVFQLYEIPSESMVPEFLVGDRVVVFKTASGPVFPLSDVGIPDLRNYKRGD
                     IVVFRNPHYDNSRKAELQSFLSQLVFMFSFTTVNLNVDENGDLKADPLVKRVCGLPGE
                     QIYLLDGNLYARTKEENAFRVVEDDSYWAAWNLHELPSDIKPKIQRMPLTNEVYKTLL
                     NIEAERRSYDLEDAARQAEAFSKRFIALKEQITGKKTDLDASFKHFLTSPEMHEYFLF
                     TQYASITKKLLTKDEGGAWFHAFLTSWTDVDLSRLDGYEEAMFKLNIMAKLIFADLII
                     RSTELIVHDSSLGIASYDDVFIGLLQKAEQLHTYMILNDSRNMPVFPPTIGDKANFLS
                     NDAYFLMGDNRFNSLDMRHSYETYAKPLTEHDPFSMYYYSNMEQREVSKKRILGTTSF
                     RFWPLSRVGIPGNHYK"
     gene            14484..14837
                     /locus_tag="PKW26_04480"
     CDS             14484..14837
                     /locus_tag="PKW26_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOQ92857.1"
                     /translation="MEIHRKLLHTEETLSRFGDDLDLYKELLSDFDYLNEFSKKRLQE
                     YLAKKNFAQLKRIIHKLKGVSGTIGAERLYDSLRDFEKLLLAEDIERLETEIIHLDDI
                     YTKTCAEIMQFLKES"
     gene            complement(14812..15549)
                     /locus_tag="PKW26_04485"
     CDS             complement(14812..15549)
                     /locus_tag="PKW26_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOQ92858.1"
                     /translation="MRYCDAHIHLAQLSVLPDMSENLFCSASHSVGEWEKNAALQKKH
                     PNHIFTSFGLHPQNPDEKLLPFLENLLGSPQPPDIIGEIGFDLFNEKYKASEKKQDAV
                     WQVCLDLAISYKKPILVHTRKAMHKIFNSVSDLKKIPAVIFHSYPGSFIEAESLLKKG
                     VHAFFSFGKPLLNGHKNALSSAAKLGLDCILLETDAPYQRLKGEKETRPEDILRVYEK
                     LANLRDIPIAVLARQIEKNFKTLLEIA"
     gene            15718..>15904
                     /locus_tag="PKW26_04490"
     CDS             15718..>15904
                     /locus_tag="PKW26_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92859.1"
                     /translation="METYVLKGHGNSQDCIRIMEEINEGYIVKIIRDRDGFEDISTDF
                     LSRDLFESCLRTGYLTKS"
BASE COUNT         5345 a         2732 c         2575 g         5152 t
ORIGIN      
        1 aaagaaagga gagaaaaata cacacgaatt gacggaatct gtacaagcgc actatcgtct
       61 tttgtgtcaa atacagtgat atttgaaatt ttcaggctat tcaaaacaga aggagacatt
      121 ttttcatagc ttataatgat accgagctct ttttctagcg caaagacaag ggcgtcctta
      181 tacgcttcaa tttttttact cattctgcct ataataggtc cagctaaaat aaaagataaa
      241 aaaacaaaaa tgagaacgat aaaactagta ataaatgctt tatttttttc tactgatatc
      301 atgcaaatgc ctgtaataca aaacaatgta ataatagcat tctttttata tttatggtag
      361 actttcacga tgatattaga taattttatt gtttttgaag gaatcgatgg ctcaggtact
      421 tccacacaat tatcgctcat aaaagagagg catcctgaca aaaagctctt tttcacagct
      481 gaaccaacaa attctcctat cggtttattt atacgaagta ttttaaaagg agagctagag
      541 atcgctcctt cgacgatggc ctatcttttt gcatcagata gaaatgaaca tctctacgga
      601 agagaaggta tccttgccca aaaagaaaat ggctttattg tttttagcga tcgatatctt
      661 ttttcaagcc tcgcctatca aaaccccatc tgtcctgaag gactcccaga aaaactcaac
      721 gaaaatttcc cgcttcccga gtaccttttt tactttgaca tagagcccga agaatcgctt
      781 tctcgagtgc atatgcgcgg tgaaacagag atatacgaaa cactttctat acaaagacaa
      841 acgcaggcag aatacaaaaa aatactgagt gcgtataaag aaaaaaacat tcctggtatg
      901 catattgtag aaatcgatgc acgtaagccg attgaagtgg tgtatcaaaa aattatggat
      961 acattggtaa atctgccgat atatactaag tgaagtctat acattttttt ggaaaaaaac
     1021 gaatatttct catcgccatc cttgtttttc tgtttattct acccagtttt tcatactttg
     1081 ttacgtataa ggagcagtac taccgccttt ttcacgtgca ttatcaacaa tatcccgatg
     1141 acattatgga aaatatttac tggttggaaa aggctgttgc tgcagatttt tctaacccaa
     1201 aatacgctct aaccaagatc gacgacgaaa aagactggga aaaataccgt tctgttttta
     1261 tgatgcacct caatctaaaa ctgatagaac aacatttacg acttggtgga aaatacgaca
     1321 agggaaaagt gtatttttac gatgctccgt ttcgagaggc acttttattt gaacttgagc
     1381 gagcagaaag ctgttatcaa gcggggcttt attactggag agaagcaaaa ctttgggctg
     1441 aaaaagcgag tgaaaaaaag ttttattttc taaacctatc tggtattcaa aactgggaag
     1501 atgagagaga aagaattata aatggcaagc ttaactatga aaaaataatt acgcgtgaac
     1561 taaagcgaat tgcagaaaac aaagcctact tacttgccat ggatgaaaat acttattaga
     1621 ctgtatacat gaaacacgaa acttttgaca tacactatcc tccgatacag cttggatgcg
     1681 aaaaaacgag tatactctat tttgaaacaa tacaagaact cacaaaaaag ctcaaagaaa
     1741 atatagataa tgccctcata gtcaccgatg atgtggtttt taatatacca ggcttaaaag
     1801 atttttttgc aggtatccct acagagacct gtctcgttct tcctgccggt gaaaaatata
     1861 aaacaataga aacagtactc tcaattttgg aacgagctat gaagctttca ttgacacgaa
     1921 aatctttatt tgtgggtata ggtggtggtg ttataaccga cctcctcgcc tttgcagctt
     1981 cgatttttaa acgtggcgct cgcctcgaac ttgttccgac aacactcctc gcaatggttg
     2041 atgctgcaat tggagggaaa acaggttgtg attttaaggg ctataaaaat agcctgggga
     2101 ccttttaccc ggcgtcaaag ctctatatat ctcctgagtt tatacaaagc cagtcagaag
     2161 atgagttccg atctggttta gcagaagtct tcaaaacagg tcttctgttt tcagaaaaac
     2221 ttgtgttact catgcaagat gaaaaagatg cgctattaaa ccgcgatcaa aggcttttaa
     2281 tgaatattat aaaacgctgt gcccaagcaa aggcgcaggt tgtagaaaaa gacttaactg
     2341 aaaaaaatat tcgaatgtgg ctgaacctag gtcatacctt tgctcatgcc ctcgaaacgg
     2401 tgcttggctt aggtaccata agccacgggg aagctgttgc ctgggggatt tcgcgcgcca
     2461 ttgccttgag tgaatcgatt ggactttgtg cggctgaata tcgggaagag gttttttcca
     2521 tgctcaaagc ctataactgg tcgacagata aaatacatcc caaatgcata gaacttaaaa
     2581 ttgaaaattg ctctgaaaaa ctcatagaag cgatgaagca agataaaaaa aatattgact
     2641 caaatattag ctttgttctt caaagaggaa taaacgacag cattattgaa aaaataagcg
     2701 aagataaaat tcgtagcatt ttatcttgac ttttttttat taaatagcta tagtacatac
     2761 atcaaatatc ggattatatc catatgcggg ggtggtggaa ttggtagaca cgctagcttg
     2821 aggtgctagt ggcttcacgg ctgtgcctgt tcaagtcagg ttctccgcaa aaaaaagagc
     2881 atttctttga aatgctcttt ttttttagtt tccctttagt acacttgttt caagcgtttc
     2941 ggctattttc ttaagctctt ccgaggcagc agtaatagct tgcgtagaaa aagaaaagct
     3001 ttcaaccccc gctgcaattt gtcgtagcgt tacaacaatt tgttcaaaag caaatgtttg
     3061 ttgctctata atgtcacgga tatcactcga agactcagcg gtaatttctg cagatgattt
     3121 tatataatta aagttttctt ctagatttgc tgaaagagta attccttctt gaattttttc
     3181 cgttccgcct tctgacgtta taatgagatt atctgaagag tgttgaattt ctgtgattcg
     3241 ctgttttatt tctcgagtag aatttgtagt taaatctgct aaacgccgaa tttcagttgc
     3301 aacgatattg aaacttttgc ctgcttcacc tgcactcgcc gcttcaagct ctgcattaaa
     3361 tgcaataatt tttgtctgat ctgtaatagt atttataata tttacgatat cccacacgcc
     3421 ttcaatctta tcgctcaaag attttatacc agtgatagtg tctttatttg catcattaat
     3481 ttcacgcatt ttttctacat ttgttgcaag ggtttttact ccatgcgtaa cattttgctt
     3541 ggtcttattt gcaacgagtg caacatcacc tattcgtgta agaatattcc ttgataattt
     3601 gtcagaatct tccatggttg caacaatttc ttttacagat gcagactgct ctaaagatat
     3661 agattctgtt tgtttagaaa taataaccaa atcctgtgtt gactttgtaa tcgatgaagc
     3721 tatcgcacca gtgttttgaa ataaaacatt gaaaatggta attattttgt ttataagata
     3781 ggcgttgaat gaaagttcat ttgacaaatc tgtatctaaa aaatcactat tctgttcttc
     3841 tctagaaaca attgacagca tagcgcttgt catgtttgca tttattttat acatatgaga
     3901 aaaataaata cgatttaaaa caatacaaaa taaaacattc aagaaaaaca ataaagcaat
     3961 acgaaacatt gagccaaatt cgtcataagc gttaagcaaa ggatttttag aaagatagcc
     4021 aaaccacaag agaataaaaa agataagact tgaaaaaaca agcggtataa tattataaaa
     4081 aacataggtt atttttagcc gagaaccaaa aaactgttta ctaaataccg ttttatcgtc
     4141 gacgcctttt tttacaatag gttttgcata ggcagaacat agattttctg ccatgttaaa
     4201 tgtaaataag gatgaaataa ttgaacccaa aagagcagca ataagagcaa aaacagattc
     4261 tgctaaagaa aaatcaacta aggcaaagca aagaaaaacc caaataatac atcccaggta
     4321 aaaaacgagg ccaacttgta ttgctatgta ttttggcaaa actaataaat cagaaagtaa
     4381 gagagttcta tcttgaatat cggtttcctt gttttgaaaa atatgcaagc gttttgaaac
     4441 agaaagggtg ataaatttat tagtaaaggg agcgacaagt tgttgaacag aaagcatgac
     4501 cactaagcct aaaatcatag ctttgggaat gaggttagtg gagacgccgg aataaataaa
     4561 aatatatggc atcaatataa gacccgatag catgtttggt aaaaaaccag aagccatcat
     4621 ttgtttttct agattttttt ttgtaatttt cattatactt ctccttttgc aactctatct
     4681 gccagacgtt caaaacattc tatgttctca gacatgtcgt aagcatgcaa ggaagcttca
     4741 tctaacaaat gttttaattg ctcagttaac gataaaatag cattgtgtat ttgcccttct
     4801 tttattttat atctttgaac gtcaggtata tctataacat ttatttctgc tttctctttc
     4861 atttcatcta aaagcatttc atttttttcc aaaaaagttt tacttttttt agttatttca
     4921 agtgagtctt gaaaatgaga gatgagtttt tctgtagtag tatgtacaag gctgatttgc
     4981 tcttgcgttt caattataga atccatagta taatcagcta agcgccgtat ttcagttgct
     5041 ataattaaaa aattttttcc gcttgcgcct gcttttactg cttcaagttc agcattaaaa
     5101 gcaataattt ttatttggtc agcaatatct gttagcaaag aagtaatttc acttattcct
     5161 gtaagttgat tttctaagct ctttaactca cgtgttgagc taaggttttt attagtattc
     5221 tgcaaagctg tatccttaga ttgactgatt aaagcgtaag cttcatctat aatgatcttt
     5281 atttgatcgt gtgtatcaaa aaaagaatcg agctcagcaa aaaaaagatc ggaactttga
     5341 tttatttttt ttacttcttc atttatatta cctataacac gatgatttgt ttctaaaatt
     5401 ggttcttgtt ctgcttgata tttttttaaa gaaaagacca attccgatat ctcagaagaa
     5461 atacgtttat aaaaatctaa aattctcgga aaacgatgta ttgtattttt tagaagaaaa
     5521 gaaatatatg aaatttcatt tgataaagca actggtattt ttttctcggt tttaaaatca
     5581 gtttttgaaa gccattcaag aaggtattgc actttagcaa tttcgagaat agaattgttt
     5641 ctaaaagcca gaaaaagcga aataagaata acagtattag taagaaaaaa gagaaccgtt
     5701 cttcctaaag aaaatatatt ttgttctaga aggatgtctt tttgtatcaa aaaataagca
     5761 aaggtaagaa cagaaacaaa caaagcgggt attatggtaa ataataaaaa ttgtattttt
     5821 agtgaaattc cataatattt tttgctttcc aattcgccta aatcgattcc agaatcaaag
     5881 atgggtattg cgtatttatg gcaaacaatt tctgaagtaa acaatgcaat aacacccgcc
     5941 acgtatgaag caaaaacaca tactgtaaat aaaaagatca gttttacgag actaattccg
     6001 ataaaaaaat acgttaacaa aataaataat aatgaggcag aaccaaaaag aataaaaaca
     6061 taatacataa ttattcttgg gcaacgtaaa agttcaacaa acaaagctgt gcttttatct
     6121 atgtcgagac ttccctcttt ccataaaatg agttgatttg aaatctgccg agtgtaactt
     6181 gcatttgtaa aaggtgcaac aaaaaactgg aagattaaaa cagaagttgt cgcaaaaagc
     6241 agtataaaac caaagtcttt aaaggaaatt tccaagaaaa acaatatata tacgaaaata
     6301 ataatagcag taatgatatt attttttgcc ccatatgaaa acatttcatc ttgaaatgtt
     6361 ttagccttag ttttttttat tttctttact ttttttatca ttttctccct ctatttactt
     6421 aaaagtgtta tagcaccatc ccacttaagg ggaggttgcg atgcaaatga tttacatcgg
     6481 ttttgcaaaa ccatcgtagc cttatcctgc ggcataatat cgagagcctt tgtaaaataa
     6541 ttataggcta aattccatgc acctaaaata tagagctcca tagcctttgc tattgtttct
     6601 tgaaattctg cagaatacgc atcttttttt tgcataacaa gataaattga tactggtatg
     6661 gacttccctt ttactcgaac agtatctaaa tggcgagtaa aaaaactttg atcgagttct
     6721 tcctttattg catttgaaat aataatcgga gcgccatatt gttttgtaag gctttctagt
     6781 cgagacgcaa gatttacaga atccccaata acggtataat ctatcttttc ttggcaacca
     6841 atatttccca caacgacatt tccttgatga atgcctatcc caatttttaa ttcatgattt
     6901 tctggaaaaa ttaaaaggct tgtatcgatt gtatgtataa cttcttgcat ctctaaagat
     6961 gcttttactg ctcgttgtgc attatcttca tgactcgttg ttgccccaaa aagtgcaaga
     7021 atagaatctc ccatgaattt gtcaacaatt cctccgttct tttttataat ttctaccatg
     7081 tgagtaaaat ataaatttaa aaaactaaca acgttctcgg gagtatttat ttcgcttata
     7141 gctgtgaagt ctcgaatatc cacaataaca acagcaacca ttctcttttc tccaacgagt
     7201 tgctttgctt caccttcatg ccctagaata ttatcgatta tttcttcagg tacaaaacga
     7261 gaaaaggttt tcttcattct catttctttc ttaataacag atgtaaaacg taaagacatt
     7321 tctattaaaa aaccaatctt ttcacaaaaa aaatgtaaca acttcaattc ttgctcagta
     7381 aaataattaa attcaacagt tcctatatga aaggttccca caaaagaatt tgcataaagc
     7441 acaaaatgtt catacgatga aatagcattt tgttttgttt tagttttctt ttcaaaacga
     7501 tatagtttct cttcattata ttctaacaaa ctaatatcaa aattatctgg aacatagctt
     7561 tcaaaatgta atttagatac tttgtaaaaa tcattttcct ctgttttggc taaactattt
     7621 gatattagag aatgtttttg aacctgagct tcatggaaaa tataaaaaga aaatatttct
     7681 acttcaaaaa tctttgcaat aagatataaa agattttcta gtaaacttgc tgtattttcc
     7741 atgtaaagat tcatttcaaa aagtgattcc aaaaaaatat aattgaaaaa gtttttttct
     7801 gttgccttaa gtgcagaaac aagcacttct ttttcagata cttcgcttag attagtttca
     7861 gaagtttcag tttcttccaa aaggagactt atctcctcgt aaagctctgc ggtattgttt
     7921 gcagtaaaaa acaaaaaagc gtccgcatgt gttttttctg cgtaaaaaga ttttaacttc
     7981 gtctcttcgc tatagtataa aagcaccttt gttttttctg tttttggctc agctttaatt
     8041 aaatgagcta aatgaaatcc cgtaaatggt tcaagatata ttgatgaaat gagtaaaacc
     8101 accggatttg actgtataaa aaagagcgca tcataggcat tgtcaaaaaa aacaatttct
     8161 tcttttatct ttcctaaaag agatttaata acattttgta caatttttga atattcacaa
     8221 acaactattt tattcttcgc catttatcaa tccttttaca attgaaagca tctttcttct
     8281 ttcaaaatgt gatttaaaaa gaaaaccgga aacccccatt tccattgtct tttcttcaac
     8341 agctcgcccc tcaagagatg aaataacaag tattggtgtt ttctcatatc ctcgaattcg
     8401 ttttagattt tctataaata catagccatt cattcgtggc atttttatat cagttacaat
     8461 aatatcatac tttcgttttt ttacgatact caaggcttca attccatctt cagtagtatc
     8521 aacaacaaag ccctctgctt ctaaaatgtt tttttgaatt tctctcgtta tcgttgaatg
     8581 atctataata agaatttttt ttacatcacg tatgttcttt gcttcagcct taattaaaga
     8641 ataggtttgt aacaattcta agcgttctat cagctcagga atatctaaaa taggtacaaa
     8701 tcgatgattt tcatccagaa ctaaaccttg aaaaatatga tactttttca aaatagacgg
     8761 aagtggtttt ataataacag aaactgtatg taataattca ttaatgttaa tacctatttt
     8821 cttttcaaga tattcaacaa tcaaaatcac ttgtgtccct ttttcttttg tatcaaccac
     8881 ctcacctata agcgcattta atgaatacac gggtaaaacc tcgtcgcgta atcgaaacat
     8941 tgtattgttt tgcaaataaa gcaaatcatt tttggggcaa ttatatattt ctgaaatata
     9001 atgaactggt ataagaaaag taaaaagtcc cgcttttata aaaacacctt ttaaagttgc
     9061 cagagaagtt ggtatagaaa gactaaattc tgtaccaaga tctttttctg ttgtaagaaa
     9121 aatctttgct ttaactttat ctgcgtttct acgaacgata tctaagccat ctccacgata
     9181 tggagattga ttagattttt gtgcaaggct aagccctggc ataaaaagaa aactcgaaag
     9241 ctctttttct gtcatttcta aaataattgc tgttttatct ggatataaat caagggcttt
     9301 aaagcgcacc gcttcatagt caatgccgca gccatcatca gaaagttcaa gagtaatatg
     9361 ttcagcatgt ttgtatatac ccaaaaatac ctgtaatctt tcttccttcc ctttttgtaa
     9421 tcgaatttct tttgtttcat atgcatggtc tattacattt tttagtaatt ggtacagtat
     9481 ttcagaaact aaatctaaga tatctttatc caaaaacaaa tcaatttctg gaaaatggaa
     9541 agctattatt ttattttgct ctaaggcttc tgcctcaatc tttttctgta aaggttctaa
     9601 aatagtactt aaagatatga ggcgaaactc atagatactt ttttgaagat tagccataga
     9661 gttttccaaa tgctccaagt catctgagaa ctcttttttt agttttttca aaaaagaaga
     9721 actgttgttt tcgagttcca cagtaatatc atctagatat ttctttaatc tattttgttg
     9781 caagactata ttttcaaatc ctttgataat ctcatctatt cgctctgtgc tcatttcaat
     9841 tgtttgaata tcgagtattt tcttttttat atcagaaggg aggagttcag gaagatcaaa
     9901 aattgatttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ctttaataat
    10021 tttatatact tcgatatttc ttttatgtat tctttcgatt catttacaaa agaagtgata
    10081 aactcatcag tttttaggaa catgtaatac caccttcttt acgaaagaaa aaaacatttg
    10141 attctttata tttttttaaa gacgattctt ttgcaatttg tatagatgct atttcatttg
    10201 tcgaaacaaa aagcaaaccc tcaggtttta atttcccttc cataaaacgt aaacatgctt
    10261 gttttgtttc atcagaaaaa tatataaaca cgtttcgcaa aaaaataaca tcaaactttt
    10321 tatcgctaag acaatcttta cagccatcaa aacgaaatag attaaaagga aaactttgca
    10381 cagtaacaag aatttctttt gaaagagaaa aatttttttg tccgtatgta agatagggtt
    10441 ccaataatgt atgaaaagca cttccatctt cccgcaaaga actaggacta tagtttcctt
    10501 tttgaagcgc ttctaaagaa aaggtattta tatcggatgc atacacctct gcatctatgc
    10561 ctaggctttt gcataaaatc gaaagggaat aggcttcttc gccagaagaa caagcagcag
    10621 accaaataga aaggcttgtg tgttttttct gtggtaaaaa aatatcgcgg agatagaaaa
    10681 aatgtttttc ttcacggaaa aaatatgttt cattgatagt gatactattg ataagtaatt
    10741 caaactcttc tttttttttc agtaaatctg catacaagag attaaaagaa aaagctctta
    10801 gtgctagtct ttttttcaag tagcgtttca aatattctac atgagaccca tttatgctca
    10861 ttccacaatg agcataaatg agttgcagaa ctgaatctat ttgtgctgtc gttaattcat
    10921 tcaccaatgc aaggagcgtt ttaggaatag cagataaggg caaaatctct tttattgctc
    10981 ccttatcaat cgcagcctta ggcataccaa aaacaacaca gctttccttc gactctgcaa
    11041 tcgtataggc accgacctct ttaagttgca gagaaccttc agcaccatcg gaacccatac
    11101 cagttaaaac aatagatata caacgattag cgaacagttt tgcaagacta aaaaaaagtg
    11161 ggtctgcggc tgggcgtaaa aaatgttttt cagcctcttt attgagtgat ataaaaaaat
    11221 ctttattaaa gtcggagcct acaactacca tgtgataatt tgcgggagcg acgtagacat
    11281 gccccttttc tattttttga ttatgctgcg caagatgcac aggaagcgga cttgtttgcg
    11341 acaaccaaga aacaaaatgg gtatcgaagt ttgtttctat atgttgcaca atgagaatag
    11401 gtaaaggaaa gtctggacca agctcttgca ataatttttg aattgcgaga ggaccgcccg
    11461 ttgaagaagc aatacctaca atctcgtata cgagtttttc acagccaata ctccgagcct
    11521 ccccttgaat agaagaatca aagcattttt cttgtgtttg aatgagaggt ctattataaa
    11581 agcctgtatt tgcctctgct atagcatgta ttttgtcaaa aaactcccta taaaaagatg
    11641 aggttagaat agataaatct ggtttttgaa taatttcgac agccccaaac tctaaggctt
    11701 tgtaagcaag atcaaatgaa tcttcagaag taaaaataac aattgcacaa ggagtttttt
    11761 ccataatgcg ttttgttgct tcaattccat ctatcaatgg catagataca tccatgataa
    11821 taacgtctgg tttcaattgt ttcgcgagct caactgcttt taagccattt cctgcctcac
    11881 ccacaaccga tataagggag tttttttgaa gactttttga gagcatgttt cgaataatag
    11941 cagaatcatc tacaagcaat actcgcgtca acttaatttc cctgccttat gtatttttta
    12001 tttctttatt gagaagttct aaaaaagaaa aagggttaat cacacttatt tcatgctcat
    12061 tgaaaagaat tgagaaagaa aaaaaagaag acctaaacat tgaatcatag gttagctctg
    12121 atatctttcc taaaaaaaaa gtttctactc cagaaacctg tatcccaaat ttttcttcac
    12181 tatcgataat tataactata ttttttactg tattttcatt ttccaataaa acatgtggat
    12241 taaaaagaac aaaaggctct ccatgtctgt ttataagccc tgcaatatat ggcggcataa
    12301 atggcaaagg gtcgatccta ggattgtaaa gaatttcttt tacacttgca ctttggaagg
    12361 catatagcgt atgagcaatt tttactatta aaaaaacttc ttgcgcttcc tccgcatcaa
    12421 ttttctctaa atcgattatt tcgacatcat cttctaatgt cttttgttct tgcaaatcta
    12481 tcactctttt ttcaactact tctttcttct ttttctcttt gcccatgatc aaagtgtagc
    12541 ttgatacatt gcagttgtca agttttttaa gaagaagaac aacaacaaca ataacaataa
    12601 aaaaagatct ttaaaaagcc ttacttataa tgatttccgg gaattccaac acgagaaagt
    12661 ggccaaaaac ggaagcttgt tgttcctaaa attcgttttt ttgaaacctc tctttgctcc
    12721 atattggaat aataatacat agaaaaaggg tcatgctctg ttaaaggctt tgcataggtc
    12781 tcataggaat ggcgcatgtc aagagagtta aaacgattat ctcccattaa aaaataggca
    12841 tcattggaaa gaaaattggc cttatcgcct atggttgggg gaaaaacagg catatttcgc
    12901 gaatcgttga gaatcatata cgtatgtaat tgttcagctt tttgcaacaa accaataaaa
    12961 acatcgtcat aggaagcaat tcctaaactt gaatcatgaa cgatgagctc agtactgcga
    13021 ataattaaat ctgcaaaaat aagttttgcc ataatattga gcttaaacat cgcttcttca
    13081 tagccatcga ggcgactgag atcaacatct gtccatgaag ttaaaaaagc atgaaaccac
    13141 gctccacctt catcttttgt caaaagcttt ttggttatac ttgcatattg tgtaaacaaa
    13201 aaatactcat gcatttcagg acttgttaaa aaatgcttaa aagatgcatc taaatctgtt
    13261 tttttccctg ttatttgttc ttttaaggca ataaagcgtt tagaaaaagc ttcagcttgc
    13321 cgggctgcat cctctaaatc ataagagcgc cgctctgctt ctatattcaa aagcgtttta
    13381 tacacttcgt ttgtaagcgg catcctctga atttttggtt ttatatcact tggtagctca
    13441 tgcaagttcc acgctgccca atagctatca tcttctacca ctctaaaagc attctcttcc
    13501 tttgtacgcg catataggtt tccatcaagc agatagattt gttctccggg caagccacaa
    13561 acgcgtttta caagtggatc agcctttaag tcgccgtttt catcgacgtt tagattcacc
    13621 gttgtaaaac taaacataaa tactaactgc gataaaaaac tctgaagttc agcttttcgt
    13681 gaattatcat aatgtgggtt acgaaagaca acaatatcgc cacgtttata gttacgcaga
    13741 tctggtatac ctacatcaga taagggaaag acaggaccag aggctgtttt gaacacaaca
    13801 actctgtcgc caacgagaaa ttctgggacc atggattcag agggtatttc gtagagttga
    13861 aatacaaaga tatttaccaa tgttacaatg agggcagctt gtacaaaggc gtctatccaa
    13921 ctaattgctt cgcctattac agaaattttc tttttaccct ttttattgag ctttatttca
    13981 ggctcggtaa aatctggatt attgatataa aaacgctttg ggttgctcag atacataaaa
    14041 acatttgaaa aaatagttac cgctacccat acaagcaccg caataaggtc aagtgcatac
    14101 gaagtatcat tattccctgc acgacgcaaa ataaaggtta ataaaagcac gaagggagta
    14161 tactcgtaaa gctttctaat aactgagaga ggaaggcttt ttctttgtga aaacaaaacg
    14221 agcccgagat acgcaagcaa taaagaaaaa ataaggcttg gtagaaaggc aagggcagaa
    14281 atatctgcat aaaacgaaag attcagtaag gtaaaacaaa atgacaaagc tataacaagc
    14341 acttgcaatg gtaaaatatg ttttttcata caactagtat agatagttta ttgaaaataa
    14401 tctacaattg aacaaaataa aattcatctt tattgtttac acagtttctg tgtatgtatc
    14461 ggggaaaaag agtatacaat tttatggaaa tacatcgaaa actattacac acagaagaaa
    14521 ctttaagccg ttttggcgat gaccttgatt tatacaagga gctactcagt gattttgatt
    14581 acctcaatga gttttcaaaa aaacgcttac aagaatatct cgcaaaaaaa aattttgcac
    14641 aattaaaacg aataatacat aaactcaaag gtgtatctgg aaccattgga gcggaacgct
    14701 tatacgatag cttgcgagat tttgaaaagc tgcttcttgc agaagatata gaaaggctcg
    14761 aaacagaaat cattcacctc gacgacatat atacaaaaac ttgcgctgaa attatgcaat
    14821 ttctaaaaga gtcttaaagt ttttttcaat ctgccttgct aaaactgcaa tcggtatatc
    14881 gcgaagattt gcaagttttt catacacgcg tagtatatct tctggtcttg tttctttttc
    14941 acccttcagc ctttgataag gtgcatccgt ttctaaaaga atacaatcta agcccaattt
    15001 tgcagcagaa gaaagcgcat ttttatggcc atttaaaagt ggttttccaa aagaaaaaaa
    15061 agcatgtaca ccttttttta aaagcgactc ggcttctata aaagaacctg gataggaatg
    15121 aaaaataact gcagggattt ttttcaaatc cgaaacagaa ttaaatattt tatgcattgc
    15181 ttttctcgta tgcactaaaa taggtttttt atacgatatt gcaagatcta aacaaacttg
    15241 ccaaactgca tcttgttttt tttcacttgc cttatatttt tcattaaata aatcaaaacc
    15301 aatctccccg ataatatctg gtggctgagg agaacctaag agattttcta gaaaaggaag
    15361 cagtttttca tcgggatttt gtggatgaag tccaaaactt gtaaaaatat gatttggatg
    15421 ttttttttgc aatgcagcat ttttttccca ttcccctaca ctatgactcg cagagcaaaa
    15481 aagattttca ctcatgtcgg gaagtacaga caactgtgct aggtgaatat gtgcatcgca
    15541 atatctcata gagaaagtat aaaacaaaaa aaataaaaag tctaagcata ttgtacaaaa
    15601 atccgatact aaaaatacat gactagtaaa acattttacg ttttatagcc ctttgggaaa
    15661 acaaaaaaga agattttttt taaactacct ataagactat ttgatgggag gttcagtatg
    15721 gaaacctatg tattaaaagg acatggtaat tcacaagatt gtattagaat tatggaagaa
    15781 attaatgaag gctatatcgt taaaataatt cgagatcgag atggttttga agatataagt
    15841 actgattttc tttcaagaga tttatttgaa agttgtttgc gaacaggcta tcttacaaaa
    15901 tcaa
//
LOCUS       DAOSSW010000036        15788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_3827_length_15788_cov_6.410221, whole genome shotgun sequence.
ACCESSION   DAOSSW010000036 DAOSSW010000000
VERSION     DAOSSW010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 15788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15788
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3827_length_15788_cov_6.410221"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..484
                     /gene="aroH"
                     /locus_tag="PKW26_04495"
     CDS             119..484
                     /gene="aroH"
                     /locus_tag="PKW26_04495"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOQ92860.1"
                     /translation="MKNSQRLYGIRGAVCVVDTEEDMQEKVSTLYETILSKNNLSEDD
                     IVSIQFTLTKDITSANPAAVLRKASWASDVPLFCSLEPDIIGSLPFVVRILITAYLDK
                     KPIPVYLHGAEVLRRDLIQ"
     gene            481..2067
                     /locus_tag="PKW26_04500"
     CDS             481..2067
                     /locus_tag="PKW26_04500"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOQ92861.1"
                     /translation="MSEKQIWIVSREYAGIAEAGGVKNVTTSLAENLVKKGFKVLVFI
                     PEYACTNKEGLENYTSSDNFSVSIPVNADKYTVSAAEADMHGVKIVFIRHAVFLEKNA
                     VYTYTKKDEAQNPEFTRGSGFKDAHILNILHQKAVLEYALFHRACPCIIHCQDAATAL
                     LSVFAHEISRYKKLCKKTGFLTTIHNAGPGYHHHFSSLDEAAYYTKLSSDILKKGLCG
                     ERVEPFLLASFYSKLSTVSPWYAEELLDPQYPYSDGLSPLFAQQNVHIQGILNGIEYD
                     KYDPKNTSQSCLSQSFNPLLGKLKGKYKIREALLEKIAKGISIEAGVDSVEQFGSIDI
                     TVENPVFFSYHGRIVWQKGLDVLAEAIPLVLTKNPQAYFIIIGQGQPELEDKHIALAN
                     QFPGRYVYLRGYERALTHECLAASDFLVLPSIFEPCGLEDFIGQIFASLPIAHAVGGL
                     QKIDHGETGFLYEDNTGQGLAEALLSAIHLFNTDRELLNAMIIKASYSVKHNYTWQSI
                     VEEYIAFYQNIVNPQSKLLT"
     gene            2125..2198
                     /locus_tag="PKW26_04505"
     tRNA            2125..2198
                     /locus_tag="PKW26_04505"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2159..2161,aa:Thr,seq:tgt)
     gene            2223..2304
                     /locus_tag="PKW26_04510"
     tRNA            2223..2304
                     /locus_tag="PKW26_04510"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2257..2259,aa:Tyr,seq:gta)
     gene            2374..2844
                     /locus_tag="PKW26_04515"
     CDS             2374..2844
                     /locus_tag="PKW26_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOQ92862.1"
                     /translation="MDLFHPKDSFQFSCVKDCIKCCSGEPGFVYLSEKDLTKLLSHFK
                     MNARDFVQKYCRFTSYYDETEVLCLQEKYNYECIFLEKNGCSVYEARPIQCSTYPFWT
                     TIVYNKKSWDREAEDCPGMNQGAFISKETAMKQNFLYEENTPITRADLELLYES"
     gene            2834..3772
                     /locus_tag="PKW26_04520"
     CDS             2834..3772
                     /locus_tag="PKW26_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ92863.1"
                     /translation="MKVKLWGVRGSLPTPLTQEMVRAKIDAVVQRMSPQDLENSDSRT
                     RFLANLPSWLYGTVGGNSSCVEVTSDEGATIILDAGTGIRSLGKKLIDSGVKTIHLFL
                     SHFHWDHIQGLPFFDPAYRSDTTLHIYSAQENAYELLDMQMQEAFFPVGLSAFTKDMH
                     FHTLSEDETFSIGSVEVQCKKMFHPGDSYSLSFSQNNKSFIYATDVEILHRDFEKNGR
                     TENFFKDASLVLIDAQYTGAEAIEKENWGHSSFCNAIDFASFANVKKILLFHHDPIYT
                     DKKLYAILDSAHWYRDYTGNHDLEIELAVEGMEFSI"
     gene            3803..3988
                     /locus_tag="PKW26_04525"
     CDS             3803..3988
                     /locus_tag="PKW26_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92864.1"
                     /translation="MHNENTNEGFFWTFSEDEAKTLVKILHKSNPIDPSIYRFYNDLL
                     LYIYERMTLEEVEILLG"
     gene            3985..4989
                     /gene="mltG"
                     /locus_tag="PKW26_04530"
     CDS             3985..4989
                     /gene="mltG"
                     /locus_tag="PKW26_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOQ92865.1"
                     /translation="MKKYSIAILIVFILGLILSLSALFVLSQGASRFYGIYEAEITIS
                     RGTSIKALASELKEKKLILNEDFFYLLARQSKTLVKAGVYSVSNELSMKEMLSLFSEG
                     RDSQIRLTIPEGYTLSKIAVLLDQKGITSYEDFMAESKNEALLQKYAIPSDTVEGYVF
                     PDTYFFSPQMEAKRVLEKMIDTFFLKISPFIEEQKLSPKEVYDTIILASIVEKEYQRE
                     EEAPLIASVFLNRLKYNIGLYSCATIVYILTEIEGREHPSYISEKDTKIQSPFNTYMY
                     AGLPPNPIANPGLVALKAAFEPAVTDYYYFRLIDEARGKHSFSKNFDEHIEVGRKVLK
                     "
     gene            5014..6810
                     /gene="dnaG"
                     /locus_tag="PKW26_04535"
     CDS             5014..6810
                     /gene="dnaG"
                     /locus_tag="PKW26_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOQ92866.1"
                     /translation="MAKISSVTIDEIDKRGDIVALVSEYTKLEQRGANWWGCCPFHNE
                     KTPSFNIMSEKNMYHCFGCGAGGGIIKFYMEMEKLSFVEAVLALAKKNNIEVIYEGNA
                     DFIPQKDSTSDAYKELYTRVSGSFHYFLTQREEGSFAKNYLLQRGFTEQSIKNFQLGY
                     APRNRKWIKTFLQQKHYSDAFINDSGLVSTKYPDYSFFSNRLMFPIHNKNGDVVAFSA
                     RLLEGEGPKYINSRESSIYKKREELYGFFQAKKRIRTEKKILLCEGNFDVIAFHQAGI
                     DYAVASLGTALTEEQIDLFSRFVDTVFLFYDDDLAGQKACQKAIFMLRKKDLTVKIIL
                     PDAGKDPAETLEISGTEGLTKLVDKAIIDIDYLLSFYAKEHSIQGPEGKAKASRAFFS
                     YVDSLHSDIQKDASLERMSQHYALKPEVVKKEYYSRGSVETSFTKQNSPLLDAQKQKR
                     IVLSAELRAMIAVLANNDCFKEFRSRISVEELDDPLAQDLYIALEESYREGSMSFESI
                     MRKTANPELESLVAKYIVSGEFSVHFKQIIDDSVLLIKRNNLEKKRDSISNQIRLSDV
                     RDREDQKQLDMLLSEKMHIDLELETLKKGKNW"
     gene            6807..8618
                     /gene="rpoD"
                     /locus_tag="PKW26_04540"
     CDS             6807..8618
                     /gene="rpoD"
                     /locus_tag="PKW26_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOQ92867.1"
                     /translation="MSSQDLEQICKDIITYGKKKKKISWEELQDFLPNDVLKDSDLME
                     EILLDLQKNKIQYIEEDVDDEDDDESDDEDEGENDDEDGEDEEENEKKKSSLVKEKKK
                     TLVNNTKVSNVDDPIKMYLREIGKENLLNAEQEVELSKKMEDGEHVIKEIIKNSGMII
                     PEFFVIAQKAFSKIDPSDSGRPRKEINEEMAERKRLRNAYGEHLKPIIPDLKQYVLLK
                     RQIYDADPTNFLFEDKVLLGHRNKILPVLQNIDIQIEEIERFSDKFIEVTKKINDYRL
                     KQERKAKELRISDYSNLRSLGRKVALKSERAKLEQELGLSADEIREIYTQIQKIDRKI
                     KRIEYEFENSIAEILDMAKEIEQGRIMMSQAKNRLINANLRLVVSIAKKYTNRGMQFF
                     DLVQEGNIGLIKAVEKFEYTKGFKFSTYATWWIRQAITRSISDQGRTIRIPVHMIEQI
                     NKVMRESRQLMQRLGREPTDEEIAEQLGWPAARVKQVRSVAREPISLETPIGEEEDSL
                     LGDFIEDKDIENPASVTSQTLLREQVHTVLSTLPPREQEVLKMRFGLDDGYSLTLEEV
                     GLYFNVTRERIRQIEAKALRRLRHPRRSNGLRDYIDI"
     gene            8668..9522
                     /locus_tag="PKW26_04545"
     CDS             8668..9522
                     /locus_tag="PKW26_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOQ92868.1"
                     /translation="MKMTQVFDKLQSLQDILTKKYELEEFINESPKKLSSQEELLARL
                     KKEYIEKNSRYEEVKQAVQELKTQLVDINASRERNEKDMDTITTHREYEVLDKAIKDA
                     TDLEQQVRKDLQKEEKILLELNEMLKQDEQLIEHQESELNAGKAILDKELESYKLEYD
                     ALLKEEEQLKPDIDPEIHFKFERIIKNKQGKGYVAVKGNVCDGCNMILPAQFANEVHA
                     GESIVFCPYCSRILFYEETAGQEDMYFQIEDTGSLADLDDDLLFDDGDEDDDDGLEEE
                     NIKSENED"
     gene            9529..9903
                     /gene="rnpB"
                     /locus_tag="PKW26_04550"
     ncRNA           9529..9903
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKW26_04550"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            9921..>10360
                     /locus_tag="PKW26_04555"
     CDS             9921..>10360
                     /locus_tag="PKW26_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92869.1"
                     /translation="MKVRVKNRIVKLSNTKKILKTIIFFILLLFLSLSIFLFIQSNQY
                     KEKKLQLSDLEKKWQNQEYAAVYASAKILLDEDQYKPEYNFYYGMSAFYLALEQIDLI
                     DAQRYIEEAIFSFRKALHRAKKKDLPVLHYLLGKSYFQKNSYSAS"
     assembly_gap    10361..10460
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10461..10706
                     /locus_tag="PKW26_04560"
     CDS             <10461..10706
                     /locus_tag="PKW26_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ92870.1"
                     /translation="EQIVLEANLLLAHILFTEKAFDEALELYTEIISVDEDNAEAYFG
                     IGLIYEEKGDLVRARAQWRRVLNIQINHSGAIQKLSL"
     gene            10741..11778
                     /locus_tag="PKW26_04565"
     CDS             10741..11778
                     /locus_tag="PKW26_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOQ92871.1"
                     /translation="MGLFSGFSTDIGVDLGTCNTLIYLRGKGIVINEPSVVAVEKGTN
                     RVVAVGAEAKRMLWKTPGDIIAIRPLADGVIADIDNTEKLIRYFISKIMPRSGLFRSI
                     RMKIGIPSCITEVERRAVQEAALKAGAKEVQIIEESLAAAIGAQIPINEPEGHMVCDI
                     GGGTTEVSVISLGGMVVTNAIRLGGDEFDEAIIKYVRNVHNLIIGQQRAERLKIEIGN
                     ATPDKNIEKMEVKGTDAITGLPRRLEIDSVEVREALKDPITQIVDEIKRTLGKTPPEL
                     AADIFTHGIVMTGGGSMLKGLQKLISKETGVPVLLVDNAIECVAIGAGQAFEVYKDMS
                     NNRSIYDSLNN"
     gene            11783..12640
                     /gene="mreC"
                     /locus_tag="PKW26_04570"
     CDS             11783..12640
                     /gene="mreC"
                     /locus_tag="PKW26_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOQ92872.1"
                     /translation="MDVKTKKRALSPHLIVFVILMVCSSTLLALSTGGFILNFKSIGF
                     SFMSSLQKGTHHLVSSVSDFFNSVKELSVLRKEYEVLTLKLQDYEYLQRNNAEIRKEN
                     ELLREQLSFSQSLAEKNYPALVIGRDPHAAYSALTIDKGSMHNIKKNMSVIAIQNGDV
                     GLVGKIVMVGLTSSMVMPVYDFQCNISARIQTTRDIGIVNGRGSANSPLLLQYVKNKV
                     IDELQFGDVIVTSGESETYTKDIPIGRIISIKNLDYDNSLEIELDPIIDFSRLETVIV
                     VDLPIRGEW"
     gene            12640..13149
                     /gene="mreD"
                     /locus_tag="PKW26_04575"
     CDS             12640..13149
                     /gene="mreD"
                     /locus_tag="PKW26_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOQ92873.1"
                     /translation="MVRTILTSIIFFFFFILLETAILSNIALLPTVPDLILILNVYLA
                     LQNGPMFGQNMGFVSGLIIDFMSAGPLGLQTLLRTIIGYSLGFFNRLINTSSFFFPAL
                     YIFLITVFKALLFIVFSFFFPQVSVLHYVFSLSFLFELLMNTLLAPFVFAFLKLFPYL
                     ILKPEFQRL"
     gene            13146..15029
                     /gene="mrdA"
                     /locus_tag="PKW26_04580"
     CDS             13146..15029
                     /gene="mrdA"
                     /locus_tag="PKW26_04580"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOQ92874.1"
                     /translation="MSNFVFQNTKDNKDFKKKHLKTILVFIIVIFGIYSLKLFSLQVI
                     QGTNFLQQSQTISQRQRFIPAQRGEIYDRNKDFPLVVNIDSFAVDLIPGEIPSGMYDT
                     VALRLAQYLGITKSSIDRKIPERDRRSYTSFEIKANIDFDLITKIAENINDLPGVSWR
                     KKPLRSYVTSNSMSHVLGYVGDITKDELKILYNKGYTNSSIIGKTGIEKQYDDLLQGS
                     AGLESRIVDVRERKLTENTMVQEPVMGNTLVLTIDSEYQQLAEKALGNRIGSAIVLKP
                     STGEVLAMVSYPYFDANMFSKEDSSAYYSTLFNDTTKPMLNRTVNAAYSPASTFKVVM
                     TAALLEEKKFPQENKIECSGIIKYGDRDFHCHVKRPGHGWLDLKNGLAQSCNIYFWTI
                     GRDYLGVDKISSYASEFGFGSSQKIDLPSQSSGFVPTAQWKERRFHEKWVGGDTMNLS
                     IGQGFLEVTPMQVANMMAMVVNEGTIYQPHLLKEVLDPVSNEAIHTVNPEVLFSSQIE
                     NEVWKTLQEALHYTATDGSASSVLMKNKVVQIAGKTGTAEVANIKDSWHSWFVAYGPY
                     DAPKDETIVVCVMVEATNEWEWWAPYASTIIFQGIFGNQSYDEAIDALGFRYLVKPVG
                     RQE"
     gene            15026..>15788
                     /locus_tag="PKW26_04585"
     CDS             15026..>15788
                     /locus_tag="PKW26_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOQ92875.1"
                     /translation="MRIFKIFEFIDLILLTLVLILIIMGVFFIYSSGVDAQNILVSNE
                     YIKQIIWAALGFILMIFFAVLDFKKIKRVLNWMVIGFIAILVYTLFFGRYVNGARSWI
                     GIGSLGIQPAEFAKTLYILFFAFYLEKTENTEPLKRFVISILILLVPMGLILLQPDLG
                     TASVYVPIFILMCYVARLPKHYIFLFFSIGMLTVLFVVLPVWEHLIVGQKVLFVSLLT
                     NKKLWLIVLAASSFITLISFLGLRIFKQRYFYWIAY"
BASE COUNT         5147 a         2700 c         3084 g         4757 t
ORIGIN      
        1 gatggttctc ttcccctaag aaacgtaaga atcttgttaa tcatgttgac tcgaacagat
       61 ttgcaaattc tttcctagca tgattgacag aagccttttc tcctcttata gttatcctat
      121 gaaaaatagt caacgattat acggtattcg gggtgcggtt tgtgtggtcg ataccgaaga
      181 agatatgcaa gagaaggttt ctactttata cgaaactatt ttaagtaaaa ataatctaag
      241 cgaagacgat attgtttcca tacaatttac ccttacaaag gatataactt ctgcaaatcc
      301 agcggctgtt ttacggaagg catcttgggc aagcgacgtt ccgctttttt gttctctcga
      361 gccagatatt attggttctt tacccttcgt tgttcgcatt ttgattaccg cttacttgga
      421 caaaaagccc ataccagtgt acttgcatgg tgccgaagtt ttacgcagag accttataca
      481 atgagtgaaa aacaaatctg gatagttagc agagaatacg caggcatagc agaagcagga
      541 ggggtaaaaa atgttaccac ctcgcttgct gaaaatttag ttaaaaaagg cttcaaagtc
      601 cttgttttta tccccgaata cgcttgcaca aacaaagaag gtctagaaaa ttatacgagt
      661 tcagacaatt tctcggttag tataccggta aatgcagata aatatactgt gagtgcagcg
      721 gaagcagata tgcatggggt aaaaattgtt tttattaggc atgctgtttt tttagaaaaa
      781 aatgctgtct acacatatac gaaaaaagac gaggctcaaa atccagaatt cacgcgtggt
      841 tcgggtttca aagacgccca tatactcaat attttacatc aaaaagcggt tttagaatac
      901 gccttatttc atcgtgcttg tccttgcatt attcattgcc aggatgctgc aacagccttg
      961 ctttccgttt ttgcacatga gatttctcgc tacaaaaaac tttgcaaaaa aacaggtttt
     1021 cttaccacaa ttcacaatgc aggaccaggc tatcaccatc atttttcatc attagatgaa
     1081 gcagcctatt atacaaaact ttcatcggac attcttaaaa agggtctctg tggagagcgg
     1141 gttgaacctt ttttactcgc ctctttttat tcaaaactaa gcactgtttc accctggtac
     1201 gcagaagaat tacttgaccc ccagtatccc tattctgatg gtctttcgcc cttatttgca
     1261 cagcaaaatg tacacataca gggtatttta aacggcatag aatacgataa atacgaccca
     1321 aaaaacacaa gccaatcttg cctctctcag agcttcaatc ccttacttgg aaagctcaag
     1381 ggaaaatata aaatacgtga agcactttta gaaaaaatag cgaaagggat ctctattgaa
     1441 gcaggtgtcg attcagtaga acaatttggt agtatagata tcaccgtgga aaatcctgtc
     1501 tttttttcct atcacggtag aattgtttgg caaaaaggct tagatgtcct tgcagaagct
     1561 attccccttg ttttgacaaa aaatcctcaa gcttatttta ttattatagg gcagggacag
     1621 cccgagctcg aagacaaaca catagccctt gcaaatcaat ttcctggacg ctatgtatat
     1681 ttgcgtggct atgaaagagc cctcacgcac gaatgtcttg cagcaagcga ttttttagtt
     1741 ttgccgagta tctttgaacc ctgcggtttg gaagacttta taggacagat atttgcaagc
     1801 cttcccatag cccatgctgt cggtggttta caaaaaatag atcatggcga aactggtttt
     1861 ttatatgagg ataatacagg gcagggctta gccgaagcct tgctttctgc tatacatctt
     1921 ttcaataccg accgggaact cttaaacgct atgataataa aagcttcgta ttcggtgaag
     1981 cataattaca catggcaaag catcgtagaa gaatacatag ccttttatca aaatatcgta
     2041 aatcctcagt ccaagctatt gacataaaaa gcgatattct atatattcaa tacattcatt
     2101 acaccaattt tgtgatgaat aattgccaac ttagctcagc tggccagagc acgtgatttg
     2161 taatctcggg gtctagggtt cgaatcccta agttggctta aagagctatg taaaatagca
     2221 atggggaggt tcccgagcgg tcaaaggggg cagactgtaa atctgttggc tcagccttcg
     2281 aaggttcgaa tccttctctc cccagtctta agtgcttctc tttttgggaa gcactttttt
     2341 ttatctattg cgaatctatt tcgaggttta tgtatggatt tatttcaccc aaaagattct
     2401 tttcagtttt cttgtgtcaa agattgtatt aaatgttgca gtggggaacc tggctttgtt
     2461 tatctttcag aaaaagattt gacaaaactt ctcagtcatt ttaagatgaa tgcacgagac
     2521 tttgtgcaaa aatattgtcg atttacttcg tattatgatg aaacagaagt gctttgcttg
     2581 caggaaaaat acaattatga gtgtatcttt ctagaaaaaa acggttgttc tgtttatgag
     2641 gcacgaccta ttcaatgcag cacctatcca ttttggacaa cgatcgtata caataaaaaa
     2701 tcatgggata gagaagcgga agattgtcct ggtatgaatc aaggagcttt tatttcaaaa
     2761 gaaacagcta tgaaacaaaa ctttttatac gaggaaaaca cacccataac aagggctgac
     2821 ttggagcttt tgtatgaaag ttaagctttg gggcgttcgt ggctctctac caactccact
     2881 gactcaagag atggtgcgag caaaaatcga tgcggttgta caacgcatga gcccccagga
     2941 tttagagaat tctgactctc gcacgcgatt tttagcaaac ctaccttcat ggctttatgg
     3001 aaccgttggg ggaaattcct cctgtgtgga agtcacgagc gatgagggag cgacaattat
     3061 tctcgatgca ggaacaggta tacgaagcct tggaaaaaag cttattgata gtggagttaa
     3121 aaccatacat ctttttttat cgcattttca ctgggaccat attcaaggct tgcctttttt
     3181 tgatccagca tatcgaagcg acacgacatt gcatatttac tcagcacaag aaaatgccta
     3241 tgaacttctc gatatgcaaa tgcaagaagc ttttttcccg gttggcttat ctgcatttac
     3301 aaaagacatg cactttcata ctctttcaga agacgaaacc ttttctatag gctctgtaga
     3361 agtacaatgt aaaaaaatgt ttcatcccgg agactcttat tctctttctt ttagccaaaa
     3421 caataaaagc tttatttatg caaccgatgt tgaaattttg cacagagatt ttgaaaaaaa
     3481 tggtagaaca gaaaattttt tcaaagatgc ctctcttgtg cttatagatg cacaatacac
     3541 tggcgctgaa gcgattgaaa aagaaaactg ggggcactct tctttttgca atgccataga
     3601 ttttgcttct tttgcgaatg ttaagaaaat cctcctgttt catcacgatc ccatttatac
     3661 agataaaaaa ctctatgcca tattggattc agctcattgg tatagagatt acacaggaaa
     3721 tcacgatctt gaaatagaac tcgctgttga aggcatggag tttagtattt aggtattaaa
     3781 aataaaaaag ataggtagta cgatgcataa tgaaaataca aatgaaggtt ttttttggac
     3841 tttttcggaa gatgaggcga aaacccttgt aaaaattttg cataagtcaa accccattga
     3901 cccaagtatc tatcgttttt ataatgattt acttttgtat atatacgaaa gaatgacatt
     3961 agaagaggtt gaaatattac tcggatgaaa aaatatagta ttgctattct tattgttttt
     4021 attttaggac ttatcctttc tctatctgct ctatttgttt taagtcaagg cgcatcgcgt
     4081 ttttatggca tttatgaagc agaaattact atttctaggg gaacaagtat taaagcgctt
     4141 gcttcagaat tgaaagaaaa aaaacttatt ttgaatgaag acttttttta tttgcttgca
     4201 aggcagtcaa agacgcttgt aaaagcaggt gtatattctg tttcaaatga actttctatg
     4261 aaagaaatgc tctctctttt ttctgaagga cgtgattcac aaattcgcct caccattccc
     4321 gaaggctata cgctttcaaa aatcgctgtg cttttagacc aaaaaggtat tacttcttat
     4381 gaagatttta tggcagaaag caaaaacgaa gctcttttac aaaaatatgc tataccctcc
     4441 gatactgtcg aaggctatgt ctttcctgac acttattttt tctctccaca aatggaggca
     4501 aagcgtgttc tcgaaaaaat gatagatacg ttttttctaa agatttctcc ctttatcgag
     4561 gagcaaaaat taagccctaa agaggtttat gacaccatta tactcgcatc catcgttgaa
     4621 aaagagtacc agagagaaga agaggcgcct cttatcgctt ctgttttttt aaatcgttta
     4681 aaatacaata taggtttgta ctcttgtgct acaattgtgt atattttaac tgagatagag
     4741 ggaagggagc atccgagtta tattagcgaa aaagacacaa aaatccaaag cccctttaac
     4801 acttatatgt atgctggttt gcctccaaat ccgattgcaa accctggttt agttgctctt
     4861 aaggcagcct ttgaaccagc tgtaacggat tattattatt ttaggttaat cgatgaagca
     4921 agggggaaac atagcttttc caaaaatttt gatgaacata tagaagttgg tcgtaaggta
     4981 ttaaagtagt agaaaaaaaa gaaagggcag tcgatggcaa aaatatcctc cgtaaccata
     5041 gatgaaattg acaaacgcgg tgatattgta gcgctcgtga gcgagtatac gaaactcgag
     5101 caaagaggcg ctaattggtg gggctgctgt ccctttcata atgaaaaaac accttcattc
     5161 aatattatga gtgaaaaaaa catgtaccat tgctttggct gtggtgctgg tggtggtatc
     5221 ataaagtttt acatggaaat ggaaaaacta agctttgtcg aagcagtgct tgcccttgcc
     5281 aaaaaaaaca atatagaagt tatctatgaa ggtaatgccg attttatccc tcaaaaagac
     5341 agtacgagcg atgcatacaa ggagctctat acgagagtga gtggtagttt tcactatttt
     5401 ttaacgcaac gagaagaagg ctcctttgca aaaaattact tactgcaacg tggctttaca
     5461 gaacaaagca taaaaaattt ccagcttggc tatgcaccgc gaaatagaaa atggattaaa
     5521 acttttttac agcaaaaaca ctactccgac gcatttataa atgattctgg tttggtatcg
     5581 acaaaatacc ctgactattc ttttttttca aatcgtctta tgtttcctat tcacaataaa
     5641 aacggcgatg ttgtcgcctt tagcgctcgc ttactcgaag gtgaaggtcc aaaatatatt
     5701 aactcccgtg agtcatcgat ttataaaaaa cgcgaggaat tatatggttt ttttcaagca
     5761 aaaaaacgca ttcgtacaga aaaaaaaatc ctcctctgtg aaggtaactt tgacgtcatc
     5821 gcctttcatc aagcaggcat cgactatgcg gtggcaagcc ttggaacagc actcaccgaa
     5881 gagcaaatcg acttgttttc acgctttgtc gatacagttt ttttatttta cgatgatgac
     5941 ttagctggtc aaaaagcctg tcaaaaggct atttttatgc ttagaaaaaa agaccttacg
     6001 gtaaaaatca ttcttcctga tgcaggaaag gatccagcag agactttgga gatttcaggc
     6061 actgaaggct tgacaaagct tgtagataag gctataattg atatagacta tttgttatct
     6121 ttttatgcga aggaacattc catacagggc ccagagggca aagcaaaggc ttcgagagcc
     6181 tttttttcgt atgtggactc gcttcattca gatattcaaa aagatgctag tttagaacgc
     6241 atgagtcagc attatgcttt aaaaccagaa gtggttaaaa aagaatatta tagcagaggt
     6301 tcggttgaga cttcctttac aaaacagaat tcacctttat tagatgctca aaaacaaaaa
     6361 cgcattgtac ttagcgctga attgcgtgca atgattgctg ttttggcaaa taacgactgt
     6421 tttaaagagt ttcgttctcg tatatctgtt gaagaacttg atgacccact tgcacaagat
     6481 ttgtacatag cgcttgaaga aagctatcgt gaaggttcaa tgagctttga atctattatg
     6541 cgtaaaacag caaatcctga gcttgaaagc cttgtagcaa aatatattgt atctggtgaa
     6601 ttttcggtgc attttaagca aattattgac gatagtgtac tattgatcaa acggaataat
     6661 ttagaaaaaa aacgtgatag tatttctaat caaattcgat tatccgatgt tagagataga
     6721 gaagatcaaa aacagctcga tatgctttta tccgaaaaaa tgcatatcga tttagaatta
     6781 gaaacattaa agaagggtaa aaactggtga gtagtcagga cctagagcaa atttgtaaag
     6841 acataattac ctatggtaaa aagaaaaaga aaatttcgtg ggaagaatta caagattttc
     6901 tgccaaatga tgttttaaaa gacagtgatc tcatggaaga gattttactc gatttacaaa
     6961 aaaacaagat tcagtacata gaagaagatg tagacgatga agatgatgat gaaagcgacg
     7021 acgaagatga aggtgaaaat gacgacgaag atggtgaaga tgaagaagaa aatgagaaaa
     7081 aaaagtcgag cctggtaaaa gaaaaaaaga aaactctggt aaacaacacg aaggtttcaa
     7141 atgttgatga cccaataaaa atgtatttac gtgagatagg taaggaaaac ttattaaacg
     7201 ccgagcaaga agtagaatta tctaaaaaaa tggaagacgg tgagcatgtt ataaaagaaa
     7261 taattaaaaa ttctgggatg attataccgg agttttttgt tatagcacaa aaagcttttt
     7321 ccaaaataga tccaagcgat tcaggtcgtc cgcgaaaaga gattaacgaa gaaatggctg
     7381 agagaaaacg cttacgaaat gcctacggcg agcacttaaa gcccattatt cctgacttaa
     7441 aacaatatgt acttctaaaa aggcaaatct atgatgccga tccaactaat tttctttttg
     7501 aagacaaagt tcttttaggg catagaaata aaattctacc tgttttacaa aatatagata
     7561 ttcaaataga agagattgaa cgcttttcag ataagtttat agaagtaaca aagaagataa
     7621 atgattatcg tttaaaacaa gagcgaaaag caaaagaact gcgcataagc gattattcca
     7681 atttgcgaag tttagggcga aaggttgctt tgaaatctga gcgggcaaag cttgagcaag
     7741 agcttggact ttccgctgat gaaatccgtg aaatttatac acaaattcaa aaaattgaca
     7801 gaaaaataaa acgcatagaa tatgaatttg aaaattccat tgctgaaatc ttggacatgg
     7861 caaaggaaat tgaacaaggc agaattatga tgtcccaagc aaaaaatcgt ttaatcaatg
     7921 caaaccttcg tttagttgta tctattgcaa aaaaatacac gaatcgtgga atgcagtttt
     7981 ttgatttagt gcaagaggga aatatcggct taataaaagc ggtagaaaag tttgaataca
     8041 caaagggttt taagttttct acctatgcaa cttggtggat tcgccaagca ataacacgaa
     8101 gtatttccga ccaaggtaga acaattcgta tccctgtgca tatgattgag cagataaaca
     8161 aggttatgag ggaatctcga caactcatgc aaagacttgg aagagagcca accgatgaag
     8221 aaattgcaga acaattgggc tggcctgcag ctcgtgttaa gcaagtgcga agtgttgctc
     8281 gtgaaccaat ttctttggaa acgccgatag gagaggaaga ggattccctc ttaggtgatt
     8341 ttatagaaga taaggatata gaaaatccag cgagcgttac atcacaaaca ctcttgcgcg
     8401 aacaagtcca tacagtgctt tcgaccctac ctcctcgcga acaagaggtt ttaaaaatgc
     8461 gttttggtct tgacgatgga tattcgctca ctttagaaga ggttggatta tattttaatg
     8521 taactcgtga gcgtattcgg cagattgaag caaaggcctt gcggcgcctt cgacatccac
     8581 gccgttcaaa tggtttacga gattatatag acatttaata cacaatggta tattctaatg
     8641 aaatgccaca aataagaaga ggtttctatg aaaatgacac aagtgtttga taaattacaa
     8701 tcgctacaag atattctcac taaaaagtac gaactcgaag aattcataaa tgaatcgccc
     8761 aagaaactgt cttcgcaaga agaattactt gcacgcttga aaaaagaata tatcgaaaaa
     8821 aatagtcgat acgaagaagt aaaacaagct gtgcaagagc tcaaaacgca attggtagac
     8881 ataaatgcat cacgagaacg taatgaaaag gatatggaca ctattacaac acatcgcgaa
     8941 tacgaagttt tggataaggc tataaaagat gccactgatt tagaacaaca agtgcgtaaa
     9001 gatttacaaa aagaagaaaa aatcttactt gaattaaatg aaatgctaaa acaagatgaa
     9061 cagttgattg aacaccaaga atctgaattg aatgccggaa aagctatttt agacaaggaa
     9121 cttgagtctt ataaattaga atacgacgcg ctattaaaag aggaagaaca attaaaacca
     9181 gatattgacc cagagattca ttttaagttt gaaagaatta ttaaaaataa acaaggtaag
     9241 ggctatgttg cagtaaaagg aaatgtctgc gacggttgta atatgatttt acctgcacag
     9301 tttgcaaatg aagtgcatgc tggtgagtca attgtatttt gcccctattg tagccgtatc
     9361 ttattttatg aagaaactgc agggcaagaa gatatgtatt ttcaaatcga agatactggc
     9421 agtttggctg acctcgatga tgatctcctt tttgacgatg gagatgaaga tgacgacgac
     9481 ggtttagaag aagaaaatat aaaaagtgaa aatgaagatt aggattttac agaaaagata
     9541 tgaccgcttg tttgtaaaaa gacaagagga aagtccgggc tccttcggaa aaaaagccgg
     9601 ttaacaaccg ggcaaattaa agaacagcaa tgctaagctt taatttgaca gatagtgcaa
     9661 cagaaaaaat accgcctgga aacaggtaag ggtgaaatgg tggggtaaga gctcaccgcg
     9721 tttttggtaa caaaaacggc tgtgaaaacc tcttttggag caagatcaag cagtaaccga
     9781 atttccgata ctgtggttac gggtagatcg ctaaagcatg atagcaatat cctgcgtaga
     9841 tagatggtca tactatattt ttcgaaatat agacagaacc cggcttatat cttttctgtt
     9901 ttttttgggg aaaggatata atgaaagtaa gagtcaaaaa cagaatcgta aaactgtcaa
     9961 atacaaaaaa aatcctcaaa acaattattt tttttatact cttactcttt ttgtccctca
    10021 gtatttttct atttatacaa tcaaatcaat acaaagaaaa aaaactacaa ctttcagatt
    10081 tagaaaaaaa atggcaaaat caagaatacg cagcggtcta tgcaagtgca aaaatacttt
    10141 tagacgaaga tcaatataaa cccgaatata atttttatta cggtatgagc gccttttatt
    10201 tggcacttga acaaatcgac ctcatcgatg cgcaacgata catcgaggaa gcaatttttt
    10261 cctttcgaaa agcccttcat agagcaaaga aaaaagacct acctgttttg cactacctct
    10321 tgggaaaatc gtattttcaa aaaaacagct attctgcatc nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn gaacaaattg ttttggaagc aaacttgctt cttgcacata
    10501 ttttatttac cgaaaaggct tttgacgaag ctttagaact ctatactgag attatttcag
    10561 tcgatgaaga taatgccgaa gcctattttg gcatagggct tatttacgaa gaaaaaggtg
    10621 atttagtaag agctcgtgca cagtggcgac gcgtcttaaa tatacaaata aatcattcgg
    10681 gagctattca aaaattaagt ttatgatata ctgtactgta tgtaaaaagg gaggctttta
    10741 atgggacttt ttagtggttt ttctactgac attggtgtgg acttgggtac ctgtaataca
    10801 ctcatatatt tacgcggaaa gggaattgtt ataaatgagc cctcagttgt tgctgttgaa
    10861 aaaggtacca atcgtgtggt agcagtcggt gcggaagcca agcgtatgct ttggaaaaca
    10921 cctggagata ttatagctat tcgccctttg gctgatggtg ttattgcaga tattgataat
    10981 acagaaaaat taattcggta ttttatctca aaaattatgc cccgtagcgg gctgtttcga
    11041 tctattcgta tgaaaatagg tataccgagc tgtattactg aagttgaaag acgtgcagtg
    11101 caagaagccg ccttgaaggc tggtgcaaaa gaagtacaga ttatcgaaga atctttagct
    11161 gcagcgatag gtgcacaaat ccctatcaat gaacccgaag ggcatatggt ttgcgatatc
    11221 ggtggtggta caacagaagt ttcagttatt tcactcggtg gcatggttgt aacaaatgca
    11281 atccgccttg ggggagatga attcgacgag gcaattataa aatatgttcg aaatgtacat
    11341 aatcttatca tagggcaaca acgcgctgaa cgcttaaaaa tagaaatagg gaatgcaact
    11401 cccgataaaa atatcgaaaa aatggaagta aaaggtactg atgcaattac cggtttgcca
    11461 cgacgtttgg aaatagactc tgttgaagta cgtgaagctt taaaagaccc aattacccaa
    11521 atcgttgatg aaattaaacg gacccttggt aaaactccac cagagcttgc agcagatatt
    11581 tttacccatg gtattgttat gacaggtggt ggttctatgc tgaagggctt acaaaaactc
    11641 atttcaaaag aaacgggtgt tccggtttta ctcgtagata atgcaataga atgcgttgct
    11701 attggggcag ggcaagcctt tgaagtgtat aaggatatgt caaacaatcg aagcatttat
    11761 gatagcttaa acaactaaaa aaatggatgt aaagacaaaa aaaagagcat taagtcctca
    11821 tctcattgta tttgttatct tgatggtatg ttctagtact cttttagctc tttcgacagg
    11881 tggttttata ctcaatttta agtccatagg cttttccttt atgtcctccc ttcaaaaggg
    11941 aacgcatcac cttgtaagct ctgtaagcga tttttttaat tctgtgaaag agctttctgt
    12001 cttacgcaaa gaatacgaag ttttaacatt aaaactacag gactatgaat atttgcaaag
    12061 aaataatgca gaaattcgta aagaaaatga acttttacgc gaacaacttt ctttttctca
    12121 aagcctggca gaaaaaaatt atcctgcttt ggtaataggg cgtgacccac atgctgcata
    12181 ttctgctttg acaatagata agggaagcat gcacaatatt aaaaaaaata tgtcggttat
    12241 agccattcaa aatggggatg ttggtttagt aggaaaaatt gtaatggttg gacttacaag
    12301 cagcatggtg atgcctgtct acgattttca atgtaatatc tctgctcgta ttcaaacgac
    12361 tcgcgatatt ggtattgtca atggaagagg atctgcaaat agtcctttgc tccttcagta
    12421 tgtaaaaaat aaagtaatag atgaattaca gtttggagac gttatcgtta cctcagggga
    12481 aagtgaaacc tatacaaaag atattcccat cggtcgtatt atttcaataa aaaatcttga
    12541 ttatgataat tctcttgaaa tagagctgga tccaatcata gatttttctc gacttgaaac
    12601 ggtcattgta gtggatctac ctatacgagg agagtggtaa tggttcgaac tattcttact
    12661 tctattattt tctttttctt ttttatcttg cttgaaactg caatactttc aaatattgca
    12721 ttgttgccca cggtccccga tttaatttta atcctcaatg tgtatttagc cctacaaaat
    12781 ggtccgatgt ttgggcaaaa catgggcttt gtttcaggtc tcattattga ttttatgagt
    12841 gccggtcccc taggcttaca gaccttgctg cgcactatca ttggctatag tttgggattt
    12901 tttaaccgct taataaatac tagttctttt ttctttcctg cgctctatat ttttcttata
    12961 acagttttta aagcactttt atttatcgtt ttttcttttt tctttccaca ggtttctgtc
    13021 ctacattatg ttttttctct ttctttttta tttgagctcc ttatgaatac cttacttgca
    13081 ccctttgttt ttgccttttt gaaactattc ccgtatttaa ttcttaagcc ggagtttcaa
    13141 agattatgag taattttgtt tttcagaata cgaaagataa taaagatttt aaaaaaaaac
    13201 acttaaaaac aattttagtc tttattatag ttatctttgg aatctatagt ttaaaattat
    13261 tttctctaca agtaattcaa ggtacaaatt ttttacaaca atcgcaaact atttcacaaa
    13321 gacagagatt tattcccgca caaaggggag aaatatacga ccgcaacaag gactttccct
    13381 tggttgttaa cattgactcc ttcgctgttg atctcattcc gggagaaatt ccttcaggaa
    13441 tgtacgacac tgttgcatta cgacttgctc aatatcttgg cattacaaag tctagtatag
    13501 atagaaaaat tcctgaaaga gatcgtcgat catatacttc ctttgaaata aaagcaaaca
    13561 tcgattttga tcttattaca aaaattgctg aaaatataaa tgatttgcct ggggtaagtt
    13621 ggcgaaaaaa acctttgcgc tcctatgtta cgtctaactc tatgagtcac gtcttgggtt
    13681 acgttggaga tattaccaaa gatgaattga aaattctcta caataaaggc tatacaaata
    13741 gcagcattat tgggaaaaca ggcattgaaa agcaatacga tgatttactt caggggagtg
    13801 ctggactaga gtcgcgaatt gtagacgtcc gagaaagaaa attaaccgaa aatacgatgg
    13861 tgcaagaacc cgtgatgggg aatacacttg tgcttacaat tgactctgag tatcaacaat
    13921 tggcagaaaa agctttgggc aatagaatag gctctgcaat tgttttaaaa ccaagtacgg
    13981 gtgaggtgct cgcgatggtt tcttatccat actttgatgc aaatatgttt agcaaagaag
    14041 attcttctgc atattattct actttattta atgacacaac gaagcctatg ctaaacagaa
    14101 cagtaaacgc tgcctattct cctgcatcta cttttaaagt cgttatgact gctgccttac
    14161 ttgaagaaaa aaaattccca caagaaaaca aaatagaatg ttctggaatt ataaaatacg
    14221 gagatagaga ttttcactgc cacgtaaaac gtccgggtca tggctggctc gacttaaaaa
    14281 atggtttagc acaatcatgc aatatttatt tttggacaat tggtcgggat tatcttgggg
    14341 tagataaaat ttcttcgtat gcctctgaat tcggttttgg aagttctcaa aaaatagact
    14401 taccctccca gtcatcaggc tttgttccta ctgcacaatg gaaagaacga cgttttcatg
    14461 aaaaatgggt tggtggcgat acaatgaacc tttccatcgg gcagggtttt ttggaagtta
    14521 ctcctatgca ggttgcaaat atgatggcaa tggttgtaaa tgaagggaca atttatcagc
    14581 cgcatttatt aaaagaagta ctcgaccctg tgagcaatga agcaattcat accgtaaatc
    14641 ctgaggtttt gttttcttct caaatagaaa acgaagtttg gaaaacgtta caagaagctt
    14701 tacattatac agctaccgat ggatccgctt cgagtgtttt gatgaaaaac aaagtagtgc
    14761 aaatagccgg taaaacagga acagcagaag ttgctaatat aaaagatagt tggcattcat
    14821 ggtttgtcgc ttatggtcca tatgatgcac ccaaagatga gaccattgtc gtttgtgtta
    14881 tggtagaagc cacaaatgaa tgggaatggt gggcacctta tgcatctaca attatttttc
    14941 aaggtatttt tggaaatcaa agctatgatg aagcgataga tgcacttggt ttccgatatc
    15001 tagtaaaacc cgtgggaagg caagaatgag aatttttaaa atatttgaat ttatcgatct
    15061 tattttactt acgcttgttt taattttaat tattatgggt gtgtttttta tctactcatc
    15121 tggagttgat gcacaaaata tactcgtttc aaatgaatat ataaaacaaa tcatatgggc
    15181 agccttgggt tttattttaa tgattttttt tgctgtcctt gactttaaaa aaataaaacg
    15241 tgtgctcaat tggatggtaa ttggttttat agcaattctt gtttatactc ttttttttgg
    15301 ccgctatgtg aacggtgcac gaagttggat tgggattggc tctttgggaa tacaacccgc
    15361 tgagtttgcc aaaacactct atattctgtt ctttgctttt tatctagaaa aaactgaaaa
    15421 tacagagcct cttaaacgct ttgttataag tattttgatt ttactagtgc caatgggact
    15481 catactttta caacctgacc ttggaacagc aagcgtctat gtccctattt ttatccttat
    15541 gtgctatgtt gctcgtttgc ctaagcatta tatattttta tttttctcta ttggtatgct
    15601 gactgttctt tttgttgtcc ttcccgtatg ggaacatctc attgtcggtc aaaaggtact
    15661 ttttgtttct ctcttaacaa ataaaaaact ctggctcatt gtcttagcag cttcttcttt
    15721 tattacactt atttcgtttc tcggtttgcg tatctttaaa cagcgctatt tttactggat
    15781 agcctatt
//
LOCUS       DAOSSW010000037        15357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_3960_length_15357_cov_7.194942, whole genome shotgun sequence.
ACCESSION   DAOSSW010000037 DAOSSW010000000
VERSION     DAOSSW010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 15357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15357
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3960_length_15357_cov_7.194942"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PKW26_04590"
     CDS             <1..661
                     /locus_tag="PKW26_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92876.1"
                     /translation="PVFSILAVFLTLSILGLGVFFFFQKQNHAQIQRADLSALNLTLE
                     EKANSLEKDIGTSLYRYILTVSELEKSYKKAQDAFQKNDDTLSQIEINRILNSNASFA
                     IKQKAQLLMQYLSEPTFDSLKTKLQYADIAKDPYLYLDCWVLWSGRLSNLLRTETKSE
                     AQLLIGYEDMKKIEGIVPVIIPANIQVDETRPLEILGKIQIIDTAIVIKIKSIYQSVY
                     D"
     gene            739..1839
                     /gene="recA"
                     /locus_tag="PKW26_04595"
     CDS             739..1839
                     /gene="recA"
                     /locus_tag="PKW26_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOQ92877.1"
                     /translation="MAKITNDFSASEDLPEKLKALEAARLQIEKQFGQGSLMKLGTAA
                     NAAGIEVIPSGSILLDEALGIGGYPRGRIIEVYGPESSGKTTLALHAIAEAQKKGGIA
                     AFIDAEHALDPVYAKNLGVNIDELWVSQPDTGEQALEIAESLVRSGAVDIIVVDSVAA
                     LTPQAEIEGDMGDSHMGLQARLMSQALRKLTGTIGKSKTILVFINQIRMKIGVMFGNP
                     ETTTGGNALKFYSSVRLEVRRFESIDAKGEEDSVGNRVRVKVVKNKVAPPFRKVELDI
                     FFGKGISAISSLLDAAVKHEFIDKKGSWYATETEKIGQGRENAIAFLTANPDFTNDLE
                     TKLREKLFPNQRLAPDKAPKNTKELVDELFEK"
     gene            1843..2394
                     /gene="folK"
                     /locus_tag="PKW26_04600"
     CDS             1843..2394
                     /gene="folK"
                     /locus_tag="PKW26_04600"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOQ92878.1"
                     /translation="MQEEKTRRRSQSVVLGLGSNRSFAFQGKNLDPLEILERACQALS
                     HVFQPEHFFYSSVYKTKAMYYENQADFYNMVVIGDYAGDPQSLLKKTQEIERLFGRDR
                     TREIPKGPRSLDIDIVLFYDEIVQSPLLEIPHPRIKERAFVLLPLLELLPESADPISG
                     QLFKESLDLLAQEDIEIHKINII"
     gene            2440..3183
                     /locus_tag="PKW26_04605"
     CDS             2440..3183
                     /locus_tag="PKW26_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOQ92879.1"
                     /translation="METAEIIKTEDAKTVKLESFEGPLDLLLFLIKKNEINIYDIPIA
                     EITEQFLDYMDYAVSTDLHSLTEFYAMAADLLYIKSRLLLPVAVDFDGEDLEDPRKDL
                     VDRLIEYQKFKKLTSLMEEKEAESEWSFERKRIQTVLPFEEEELWEKIDTWDLLKTFS
                     TLVSSYNSERILDLYEEISVSEKITLMNELLEERGECFFTDLIVRKENLLDIVCAFMA
                     ILEAVKFKMASIYQNRMFGDIKIKPFEAA"
     gene            3185..3730
                     /gene="scpB"
                     /locus_tag="PKW26_04610"
     CDS             3185..3730
                     /gene="scpB"
                     /locus_tag="PKW26_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOQ92880.1"
                     /translation="MEFDLEKETALIEAILFLEGEPLDENALAKISELSKDVVLLCID
                     ALHKKYEEEHSGFELVQISGGWVLSPKKDLWDFLKERYGKKNEARLSKAAMETLSIIA
                     YSQPITRSEIEAIRGVSADNMIRLLVERNFIREVGKKDIPGKPVQFGTTKDFLKFFRL
                     NSIADLPKLDETESERFELAR"
     gene            3693..4481
                     /locus_tag="PKW26_04615"
     CDS             3693..4481
                     /locus_tag="PKW26_04615"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOQ92881.1"
                     /translation="MKQKVRGLNLPDKKKDARPGKNSDIAKEDMFPLRLQVYLARCGV
                     ASRRGSEAFIIAGRVSVNGITVSELGTKVEKGDSVLVDGKPIQLEEKKRYVLLHKPAG
                     FVCSAKDEKGRQSALDLLKPHFTERLFTIGRLDMFSSGLLLCTNDGDFAAKLAHPSSG
                     LEKEYLVVTTTPIPQELLRQFVKGIRIADVFYKCKAAELVNPYTMRITLVEGKNREIR
                     RVCDHFNITIKRLIRTRIGFLGLDGLDVGFHRELGADEVKKLAL"
     gene            4507..5139
                     /gene="cmk"
                     /locus_tag="PKW26_04620"
     CDS             4507..5139
                     /gene="cmk"
                     /locus_tag="PKW26_04620"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOQ92882.1"
                     /translation="MVIAIDGPAGTGKSTIAKRIADYFSISFLNSGSFYRGICLLLLE
                     NKKDLENAAEVCDYALRLHIDYIDERLVIDGRDVEDLLHSDMVDRHVAQLSAIPELRS
                     IVNEKIRAITKSQSIVCEGRDMTTVVFPDADYKFYLDASLDVQAQRRFDQGVSDLSLE
                     EIKETIRKRDLIDKGKSVGALKRAADAMYIDTSDLTIDEVCAIIVSKINI"
     gene            5149..6852
                     /gene="rpsA"
                     /locus_tag="PKW26_04625"
     CDS             5149..6852
                     /gene="rpsA"
                     /locus_tag="PKW26_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOQ92883.1"
                     /translation="MEQMEVEKNEVDIQTQLQEEYLKSLDALEEGQLVEGTVVQVSNE
                     RVFVDVGGKSEGEIPAIEFTELPEVGDVVSVVVVNKENKHGAVVVSKQKADTKHLWKN
                     LKQAHTDKTLVEGTIDKVIKSGYEVDLSYGVKAFLPISQSDSQKVDDPESLVGIKSMF
                     YIERLYSDGKSNIVVNRRKYLEELIDTNRETFFSTVAVGDVVKGVVKSFTSFGAFIDL
                     GGFDGLLHINDMSWGHVTRPKDYVKKGQEIELKVIRLDSEEKRINLSLKHFTDDPWLH
                     FEDKYQVNAIVKGKVTKLTEFGAFIELEDGIEGLAHISEFSWVKKINKPQEVVKIGEE
                     VDCMILGYDIQAGRVSLGLKQVTENPWDSIVESYPVGTKLSKKVVKLTNAGAFIELEE
                     GIDAFLQLDDWSWTKRIKTFANELEVGQEIDCVIVENNPESKRIRVSVKDLEENPWVE
                     FAKTYKSGSIVEAEITSIMDFGIFVKAPLGIEGLINKSNLSDSREESFEEAVKKYTVG
                     DTISVYVVDVNPGKEKAAFSVKEYKQKMQRDEMSQYLSQGGGENADAYTLGDMLKNKT
                     N"
     assembly_gap    6930..7029
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7030..8088
                     /locus_tag="PKW26_04630"
     CDS             <7030..8088
                     /locus_tag="PKW26_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOQ92884.1"
                     /translation="ELLSTQASIAYNNAQTYTQAQKEISVLQTAVNAGPEYHTFVAES
                     KPVLDLLSVIDRAAETNSSVLILGESGVGKELFAEQLHLKSSRRKKPFIRVNCAALSP
                     QLLESELFGHVKGAFTDASQNRLGRFEAADGGTIFLDEIGELPLEVQVKLLRVIQSKT
                     FEKVGSSTTQKVDVRIIAATNRNLEEMVAEGRFRSDLYYRLNVLPLLVPPLRNRKDDI
                     KPLALFFCRKFSSETKKDFTGFTKEALNSLYSYYWPGNVRELENSIERACVLGKPPLI
                     TERDLLLQSTNHTETALSFNFDLQDKTLKTVMNECKKNYIIKILEEVQWNQTEAAKVL
                     DVQRTYVSKLIKDLQITL"
     gene            8100..8795
                     /locus_tag="PKW26_04635"
     CDS             8100..8795
                     /locus_tag="PKW26_04635"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ92885.1"
                     /translation="MAAKNFTEKDAKKTISKKVSEFLSKQRILLISIPLALILIAGLY
                     GVVTLVNNSVAKKDFENLDTILFSLEKKAMGASEDTLLQSIHETKQELQAFVAKKKKG
                     AASARASMALAGLEFEEENYAEAMDAYLASAEANKKAYTASVAYYNAAICAEELGQLE
                     KALEIFEKAKSSPHFALVPQTLFNMGRIALELQDKEAAKGYFQTLVDKYSNESWANLA
                     KSQLLILETEGNL"
     gene            8811..9503
                     /locus_tag="PKW26_04640"
     CDS             8811..9503
                     /locus_tag="PKW26_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92886.1"
                     /translation="MKKKGYQGILRILFGFSLILFFSSCGLPVYYIIYPPVNAGSPTD
                     IQDGRYFSFKTADQLNKTTDIYRGIDVYYKIYNSEKDRDSDIQQIQAANSEFSQSGFT
                     KLKSLGYAKLISKPSLHDSTDILFDKENSDANIKIRLFDEGEYKAGFKINDIVPSESK
                     PFRSNGNSFQFPYENLPIQGDTDYKHNEEESDYFWVAAFAVSVGRDGFFQAYYSSLLP
                     LGSIKIPKDTSN"
     gene            9569..9642
                     /locus_tag="PKW26_04645"
     tRNA            9569..9642
                     /locus_tag="PKW26_04645"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9603..9605,aa:Arg,seq:tct)
     gene            complement(9676..10041)
                     /locus_tag="PKW26_04650"
     CDS             complement(9676..10041)
                     /locus_tag="PKW26_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOQ92887.1"
                     /translation="MMTKSLEDYLEAIGFLSEENKTPRVIDIAKRLGLSKPSVHAALH
                     NLVDRGMIEHEHYGPIQLTEQGKQKYQELKEKHESLTAFFISLGVSPDVAECDACAIE
                     HVISDESFEKIKELMKERK"
     gene            complement(10049..12121)
                     /gene="priA"
                     /locus_tag="PKW26_04655"
     CDS             complement(10049..12121)
                     /gene="priA"
                     /locus_tag="PKW26_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOQ92888.1"
                     /translation="MIFVEVLFNVPINQSFTYTVSETTKLPKIKGEKPFEIQAGLRCE
                     TYFGNRKMTGFIISVKEVRQEEIASLNLGVPLEKIKSLLRIIDSERVFTDELIDLSKK
                     IARFYLCAHGEVLSAMIPSGRRASDGGMGFSTGFTEEASYAEKNLSQEQERAIEDIFA
                     GKENNTLLHYLFGHTGTGKTEVFLQLAKKTLAEGKSVIYLVPEIGLTHQVIEEIRERF
                     GDSLAVLHSALSIAQKIAEWKKIIQGKAKIVVGARSAIFAPVENLGLIIIDEEHDASY
                     KAGNTPRYHARQVAMMRSSRHQSLLVMGSATPSLEAWHAMSEKIIIQHKLTERLSGGK
                     KPEMQVVNLSCTDMSCSSISPELEENMREVLKEERQVMLFLNRRGFTHFFKCNSCGKD
                     LQCKNCSVSLTYHKSEKRFRCHYCGWTMEAQNSCPSCNSLDIAYYGFGTEFIEKEVQA
                     KFPGYSIERLDTDSLENKNDLAEKLERFRNGKTSILLGTQMVAKGLNFPGLKLVGVVL
                     ADTGLHMPDFRANERVFSLLVQVAGRAGRFFPDGKVIIQSFYPERPAIHFACNTDIEG
                     FYKWEIEQRKSLFFPPFSRLISLVFRSASKEKSAEAAESAYTILEAELEALKQKNAPK
                     DEEYWDEILGPSPAPLAMIAQNHRHQILLRATELKYIHTAVERFLSHYKPISGVYIEV
                     DLDPLNLL"
     gene            complement(12118..12960)
                     /locus_tag="PKW26_04660"
     CDS             complement(12118..12960)
                     /locus_tag="PKW26_04660"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOQ92889.1"
                     /translation="MNKISFEKAQKLWENLNTIESAFLGYTVKDDLNSFIEKMPQKPS
                     FTKNYPQHFQKTEYPRIQKPQENLGSGHIQENTRTTNIDTSSSLYAKMQECTRCALHQ
                     SRKQAIEGRGVKNPLVLVITEAPTMQDDESGIPMSGEDGRLLSKMLQAISLSEDTNTY
                     ITPIIKCRPPENKLPLLEQMYACKTFLDIQIQKMQPHFILALGSLTSLCLLGPKQNFQ
                     DIRGKVFDYKASSLQKESTPLLVSYSPLTLLHDEALKRPAWQDLKLVRRVLDDILMME
                     GKNK"
     gene            13041..14663
                     /locus_tag="PKW26_04665"
     CDS             13041..14663
                     /locus_tag="PKW26_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92890.1"
                     /translation="MKRYLFLFYCILLVFSLFALNDGQRLVPAGHWVYESLWKLSLET
                     GEVPLLNTAPVSRAEIDRYMTALDTARLSVAGVQERERVLGFLNKGTFSYNPSPFTVG
                     IDPSLHIESYYKSSTEIPWVYDNKEKKHLLDLPIIISLENYLTAESNFFLANNYWTML
                     EADNVLNIPLGDMDPDIHFPDFAYISFGLPFSGNFSFNAQLGKGALSLGKTQLGSIIL
                     NDSFYHDAYAVFSLYSPRFRYAAYTLQAEVNKYLYMHKMDFNFKRFQFSILEGAMVAA
                     PLELRFLNPVMIFHNYAAWRSYADYDEEHEKIESRVGSYFAASFDYNPWKNMRLYGIF
                     AFNQFQLPFERKNFGDAAASIPDSLGFQGGFETSLPYKAGYFVGGIEGVYTSPWFYFT
                     ESKESSFYSKRLDLIKNTSKNINSWVGFPFGPDTIAGNFYFGYDKGGDWSAKIFYRFL
                     AQGEMSGEKGFAIFENPGFYPSNPKEALITSPSGIAQFSHTIGIGGLYKPLSYLEISS
                     QLAYTFIINKNNIKNNNGSGVELVLSTSFLLP"
     gene            14678..15028
                     /locus_tag="PKW26_04670"
     CDS             14678..15028
                     /locus_tag="PKW26_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92891.1"
                     /translation="MFQKKILSCLVFVLLLTLVFGQVATSPNDPLYDDILIWENLLLI
                     PNQSPVRPYPLATIKTILDRVIASSNSLQASIAEAITRESLENPSLFLLATNHVLNFR
                     TLEKGLCNKTYFRL"
     gene            15001..>15357
                     /locus_tag="PKW26_04675"
     CDS             15001..>15357
                     /locus_tag="PKW26_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92892.1"
                     /translation="MQQNILPAVEGDFFLTEKTSLAFDLGFHVIDDYAASYSKQFTND
                     SILPFFSRPKHDAPDDPAELASFSALTNMNMNLAYGGERWYMQGGMSRLSFGPFHTES
                     VTLSSEGYHSGNLIFYY"
BASE COUNT         4704 a         2702 c         3201 g         4650 t
ORIGIN      
        1 cccagttttt tctatcctcg cagttttttt gactttatct atacttgggc ttggtgtttt
       61 tttctttttt caaaaacaaa atcacgcaca aatacaaaga gctgatttat ccgctctcaa
      121 tttaacactg gaagaaaaag ccaatagttt ggaaaaagac ataggcactt cgttatatcg
      181 ctatattttg acagtgagcg agcttgaaaa atcttataaa aaagcacaag acgcatttca
      241 aaaaaacgac gatacgcttt cacaaattga aataaaccgt atcctcaact caaatgcttc
      301 atttgcaatt aaacaaaagg cgcaactttt gatgcagtat ttatctgagc cgacctttga
      361 ttcattgaaa acaaagcttc agtatgcaga tattgcaaaa gatccgtatt tgtatttaga
      421 ttgttgggtg ctgtggagtg gcagactttc aaatcttctg cgaactgaaa caaagagcga
      481 agcccagtta ttgattgggt atgaggatat gaaaaaaatc gagggcattg ttcctgttat
      541 cattccagcc aatatacaag tcgatgaaac acgccccttg gaaatactgg ggaaaataca
      601 aattatcgat acagctattg ttattaaaat aaaatcaata tatcagtcag tctatgatta
      661 gtttttttta ttttttataa aatttttaaa aaataaaaaa aactaaggca aaacgccact
      721 aatataaggg aggacagtat ggcaaaaata acaaatgatt ttagtgcttc tgaggattta
      781 ccggaaaaat taaaggcttt agaagcagct cgtttacaga ttgaaaaaca gtttgggcaa
      841 ggttccctca tgaagctagg gactgcggca aatgctgcag ggatagaagt aattccttct
      901 ggatctatct tgctcgacga agctttggga atcggtggtt atccgagggg acgaattatt
      961 gaagtgtatg gacctgaatc ttcaggaaaa acaaccttgg cacttcatgc aatcgcagaa
     1021 gctcaaaaaa agggtggcat tgccgcattt atcgacgcgg aacatgcctt ggatccagtg
     1081 tacgcaaaaa acctgggagt aaacatcgat gaactttggg tatcgcaacc tgatacggga
     1141 gaacaagccc ttgaaatagc agaaagcttg gtgcgatccg gtgccgtgga tatcatagtc
     1201 gtggactccg ttgcagctct tactccacag gcagaaatcg aaggagacat gggagactca
     1261 catatgggct tacaagctcg tttgatgagc caggctctgc gaaaacttac gggaactatc
     1321 ggaaagtcaa agacaatttt ggtctttatt aaccaaattc gtatgaaaat aggtgttatg
     1381 tttggaaatc ctgaaacaac aactggtgga aatgccctta agttttattc ctctgttcgt
     1441 ttggaagtac gacgttttga atctattgat gcaaagggtg aagaagactc cgttggaaac
     1501 cgtgttcgtg taaaagtagt gaaaaataag gttgccccac cttttagaaa ggtagaactc
     1561 gatattttct ttggaaaggg tatttctgct atttcaagct tactcgatgc agctgtaaaa
     1621 catgaattta tcgataaaaa aggttcgtgg tatgctacgg aaacagaaaa aattggtcag
     1681 gggagggaaa atgcaattgc gtttttaaca gcaaaccctg atttcacaaa tgaccttgaa
     1741 acgaagcttc gagaaaagtt gtttcctaat caaaggctcg ccccagataa ggcaccaaag
     1801 aatacaaaag aacttgtaga cgagcttttt gaaaaataaa atatgcagga agaaaaaaca
     1861 cgaagaaggt cccagagcgt tgttttggga cttggctcca ataggtcttt tgcctttcaa
     1921 ggaaaaaatc tcgatcccct tgaaatccta gagcgtgcat gtcaggcgct ttcccatgtt
     1981 tttcagcctg agcatttttt ttattcatcg gtgtacaaaa caaaggctat gtactacgaa
     2041 aaccaagctg atttctataa catggtagtg attggcgatt atgcaggaga tccacaatct
     2101 ttgctaaaaa agacgcagga aatagaaaga ctttttgggc gagataggac gagggaaatc
     2161 cccaagggac cacgcagtct cgacattgac attgtcttgt tttatgatga gattgtgcag
     2221 agcccgcttt tggagatacc acatccacga atcaaggaaa gagcctttgt tttgcttcct
     2281 ttacttgagc ttttacccga atctgccgac cctataagtg gacagttgtt taaagaatca
     2341 ctggacctat tggctcaaga ggatattgaa atccataaaa tcaatatcat ataaaagctt
     2401 tataaaaaaa cgctgtttta aaattttata ggggtagata tggaaactgc agaaataata
     2461 aaaactgaag atgcaaaaac agttaaatta gagagttttg aaggaccact cgatttatta
     2521 ttgtttttaa ttaaaaaaaa tgagataaat atttacgata tacccattgc agagataacc
     2581 gaacaatttt tggattatat ggactatgcg gtatcgacag atttgcatag cttaacagaa
     2641 ttctatgcga tggctgcaga tcttttgtat attaaatcga ggcttttact acctgtagcg
     2701 gttgattttg atggcgagga cttggaagac ccacgcaagg atttggttga caggctcatc
     2761 gagtatcaaa agtttaagaa gttgacgagc ttgatggaag aaaaagaggc agaatcggag
     2821 tggagttttg agcgaaaacg catacaaact gttttgccct ttgaagaaga agaattatgg
     2881 gaaaaaatag atacttggga cttgttaaaa accttttcta cccttgtttc ttcatataat
     2941 tctgaaagga ttttggattt atacgaggag atttctgtaa gtgaaaaaat aacacttatg
     3001 aatgaacttt tagaggaacg gggagagtgc ttttttaccg acttaatcgt gcgcaaggaa
     3061 aacttgctcg atattgtctg tgcttttatg gcaattttag aagcggttaa atttaaaatg
     3121 gcaagtattt atcaaaacag gatgtttgga gatattaaaa taaagccctt tgaggctgca
     3181 taaaatggag tttgatttgg aaaaagaaac agcattgatt gaagcgattt tatttttgga
     3241 gggagagccc ttggatgaaa atgctttagc aaagatttca gagctttcca aggatgttgt
     3301 tttactatgt atagatgcct tgcataaaaa atacgaggaa gaacattcag gttttgagtt
     3361 ggtgcaaatt tctggtggct gggttttgag ccctaaaaaa gatttgtggg actttttgaa
     3421 agagcgatac gggaagaaaa atgaggcccg cctttcgaag gcagctatgg aaacgctttc
     3481 gattatagcc tattctcaac ccataactcg ctcagaaatc gaggcaattc gtggcgtctc
     3541 tgccgacaat atgattcgcc tcctcgttga gcgaaatttt atccgtgagg tggggaaaaa
     3601 agatattcct gggaaaccgg tgcagtttgg tacaacaaaa gactttctaa agttttttag
     3661 attaaatagt attgcagatt tacctaaact cgatgaaaca gaaagtgaga ggtttgaact
     3721 tgcccgataa aaaaaaagat gctaggccag gaaaaaatag cgatatagca aaagaagata
     3781 tgtttcccct acgtttgcaa gtctatttag ctcgctgtgg agtagcaagt agaagaggaa
     3841 gtgaggcttt tattattgca ggaagagtga gcgtaaatgg aatcactgta agtgaactgg
     3901 ggacaaaggt tgaaaaagga gatagtgttt tagtcgacgg aaagccaatt caactagaag
     3961 aaaaaaaacg ctatgtgctt ttacacaagc cagctggctt tgtttgttcc gcaaaagatg
     4021 aaaaaggtcg gcaatctgcc ctagatttat tgaaaccgca ttttactgag cgattgttta
     4081 cgattggtcg cctcgatatg ttttcttcag gcttactttt gtgcacaaat gacggtgatt
     4141 ttgccgcaaa actagctcat ccatcttcag gcttagaaaa agaatatctt gttgtaacga
     4201 ccactcctat tccgcaggaa ctgctgcgac agtttgtgaa aggtattcgc atagcggatg
     4261 ttttttataa gtgcaaggct gccgaattgg tcaatccata taccatgcgt attacattgg
     4321 tggaagggaa aaatcgtgaa attcgtcgcg tctgtgatca ttttaatatt acaataaagc
     4381 gccttattcg aacgcgtatc gggtttttag gtcttgatgg attagatgtt ggctttcata
     4441 gagaactagg tgcggatgaa gtcaaaaaac ttgccttata aaataaaaat atagagaggt
     4501 tttcctatgg ttatagcaat tgacggtcct gcaggaacgg ggaaaagtac tattgcaaaa
     4561 cgcattgccg attattttag tattagcttt cttaactcag ggagttttta tcgtggtata
     4621 tgcttgctat tgcttgaaaa caaaaaagat ttagaaaacg ctgctgaagt ttgtgattat
     4681 gcgcttcgtt tacatattga ctatatcgat gaaagactcg tcatagatgg aagagatgtt
     4741 gaagatcttc tccatagcga tatggttgat cgccatgttg ctcagctttc tgctattcct
     4801 gaactgcgta gcattgtaaa cgaaaagatt cgcgctatta caaaatcgca aagcattgtc
     4861 tgcgagggga gggatatgac gactgttgtt tttcccgatg ctgactataa gttttatctt
     4921 gacgcttcct tggatgtgca agcacaaaga cgttttgatc aaggagtaag tgatttaagc
     4981 ttagaagaaa taaaagaaac aattcgaaaa cgcgatctca tcgataaggg aaaaagtgtt
     5041 ggtgcattaa aaagagcggc agatgctatg tatatcgata cttctgactt gaccatagat
     5101 gaggtttgtg cgataattgt aagcaaaata aatatataag ggttttttat ggaacagatg
     5161 gaagtggaaa agaatgaagt tgacattcaa acacaattgc aggaagagta tcttaaaagt
     5221 ttagatgctc ttgaagaagg gcaacttgta gaaggtaccg ttgtacaagt ttcaaatgaa
     5281 agagtttttg tcgacgttgg tggcaaatca gagggagaaa ttcccgcaat cgaatttacc
     5341 gagctaccag aagtaggcga cgtggttagc gttgttgtcg tcaataaaga aaataagcac
     5401 ggtgctgttg ttgtatcgaa acaaaaggct gatacaaaac acctttggaa aaacttaaaa
     5461 caagctcaca ccgataaaac attggttgag ggtactatag ataaggttat aaaaagcggt
     5521 tacgaagtag atttaagtta cggcgtaaag gcttttttac caattagcca atcagacagc
     5581 caaaaggttg atgatccaga atcacttgtt ggaataaaga gtatgtttta tatcgaacga
     5641 ctctattctg acggcaaatc aaatatcgtt gtaaaccgaa gaaaatacct cgaagaactc
     5701 atagatacaa atcgtgaaac cttcttttct actgttgctg ttggtgacgt agtaaaaggt
     5761 gtagtaaaga gctttacaag ctttggtgct tttatagacc tcggtggttt tgatggtctt
     5821 ttacatataa acgatatgag ctggggacat gtaacacgtc caaaagatta tgtgaaaaag
     5881 ggtcaagaaa tagaattaaa agttatacgc cttgactccg aagaaaagcg cataaatctt
     5941 tctctaaaac actttactga cgatccatgg ctccattttg aagataaata tcaagtaaat
     6001 gctattgtaa aaggcaaggt tacaaaactt acagaatttg gtgcttttat tgagcttgaa
     6061 gatggtatcg aaggacttgc gcatatctca gagttttctt gggtgaaaaa aatcaacaag
     6121 cctcaagaag ttgttaagat tggcgaagaa gtagactgca tgattttagg ctatgatatc
     6181 caagcaggac gagtatcttt ggggcttaag caagtgactg aaaacccttg ggattctatc
     6241 gtcgagtctt atcctgttgg tacaaaactt agcaaaaaag tggtaaagct taccaatgcc
     6301 ggtgcattta ttgaacttga agaaggcata gacgcattct tgcaacttga tgactggtca
     6361 tggacaaaga gaattaaaac ctttgcaaat gaattggaag ttggtcaaga gatagactgc
     6421 gttattgtag aaaataatcc tgagtcaaaa cgtattcgag tgagcgtaaa agaccttgaa
     6481 gaaaatcctt gggttgaatt tgcaaaaacc tataagtcag gttctatcgt tgaagcagaa
     6541 ataacttcaa tcatggactt tggtattttt gtaaaagctc cattgggcat cgaaggttta
     6601 atcaataaat caaacctcag tgattcccgt gaagaaagct ttgaagaagc tgtgaaaaaa
     6661 tacacggttg gtgacaccat ttctgtctat gtcgttgatg tgaatccagg caaagaaaaa
     6721 gctgcttttt ccgtaaaaga atacaagcaa aaaatgcaaa gagatgaaat gtctcagtat
     6781 ttatctcaag gtggtggtga aaatgcagat gcctacaccc ttggcgatat gctaaaaaac
     6841 aaaacgaatt aatgagtagc gatgagcgag aaatcgatga aagctattga cattgaaaaa
     6901 ttaaatacac tcgtcgaaat aaactcgctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnng aattgctttc cacgcaagct tctattgcct ataacaacgc tcaaacctat
     7081 acgcaagcac aaaaagaaat ttctgttttg caaaccgcgg taaatgccgg acctgaatac
     7141 catacctttg tcgcggaaag taaaccagta ctcgatttgc tttctgtaat tgatagagca
     7201 gctgaaacaa actcttctgt tttgattttg ggtgaaagcg gtgttggaaa agagcttttt
     7261 gcagagcaat tacacttaaa aagttcgcga aggaaaaaac cttttatccg tgtaaattgt
     7321 gccgcccttt ccccacagct tttagaaagt gaactttttg gccatgtaaa aggtgctttc
     7381 accgatgcga gccaaaacag acttggacga tttgaggcgg ccgacggtgg caccatcttt
     7441 ttggatgaga ttggtgaact tccgctcgaa gtacaagtga aacttttgcg tgttatacag
     7501 tcaaaaacct ttgaaaaggt tggctcgagt acaacacaaa aagttgatgt gcgaattatt
     7561 gcagcgacaa atagaaatct tgaggaaatg gttgcagaag gtcggtttcg aagcgattta
     7621 tattatcgtt tgaatgtttt acccttgctt gttccccctc tacgaaatag aaaagacgat
     7681 ataaaaccgc tcgctctttt tttctgtcgt aaattttcct cggaaacaaa gaaggatttt
     7741 actggcttta caaaagaggc tttgaattct ttatattcct attattggcc ggggaatgta
     7801 cgcgagcttg aaaactctat cgaaagagct tgtgtgcttg gaaaaccacc gcttataaca
     7861 gagcgtgatt tattgttgca gtctacaaac catacagaga cagcgctgtc ttttaatttt
     7921 gatttgcagg ataaaacatt gaaaacggtg atgaatgaat gcaaaaaaaa ctatataata
     7981 aaaatactag aagaggtaca atggaatcaa actgaagccg caaaggtttt ggacgttcaa
     8041 cgtacctatg tttcaaaact aataaaggat ttacaaatta cactttaagg ggcaaaaaaa
     8101 tggcagctaa aaattttaca gaaaaagatg caaaaaagac aatttcaaaa aaagtgagtg
     8161 aatttttatc aaaacagaga attttactta taagtatacc gcttgcttta attcttattg
     8221 caggccttta cggagttgta acactggtta acaactcagt cgcaaaaaaa gattttgaaa
     8281 atttggatac tattttattt agcttagaaa aaaaagcaat gggtgcatca gaagatacat
     8341 tattgcaaag tattcatgaa acaaagcaag aattgcaagc ctttgtagct aaaaagaaaa
     8401 aaggtgctgc aagtgctcgt gcttctatgg cgcttgctgg tttagaattt gaagaagaaa
     8461 actacgcaga ggctatggat gcttatcttg caagtgcaga ggctaacaaa aaagcctata
     8521 cagcttctgt tgcctattat aatgcagcaa tctgtgctga agaacttgga caactagaaa
     8581 aagctctaga gatttttgaa aaagcaaaaa gctctcctca ttttgcactt gttccacaaa
     8641 cattattcaa tatgggaaga attgccctag aattgcaaga caaagaagct gcaaaaggct
     8701 attttcaaac gcttgttgac aaatattcga atgagtcctg ggcaaatctt gcaaagtcgc
     8761 aattattaat cttggaaaca gaaggaaatc tctaatccag tgagtttttt atgaaaaaaa
     8821 aaggctatca aggcatactt cgtatattat ttggtttttc tttgatactt tttttttcat
     8881 cctgtggact cccggtatac tatataatct atccaccagt aaatgctggc agtcctactg
     8941 atattcaaga cggaagatat tttagtttta aaactgctga tcaactaaac aaaacgactg
     9001 atatctatcg tggcatagat gtgtattata aaatatataa tagcgagaaa gaccgtgatt
     9061 cagatattca gcaaatacaa gcggcaaatt ctgagttttc gcaatccggc tttactaaat
     9121 tgaaaagtct tggttatgca aaattgattt cgaaaccctc attgcatgat agtactgata
     9181 ttttatttga taaagaaaat agcgatgcta atataaaaat tcgtttattt gatgagggtg
     9241 agtataaagc tggcttcaaa ataaatgata tagttccttc tgaaagcaaa ccttttcgtt
     9301 caaatgggaa cagttttcaa tttccttatg aaaatctacc gatacaaggt gatacagatt
     9361 ataagcataa tgaagaagaa agtgattatt tttgggtagc agcttttgct gtttcagttg
     9421 ggcgtgatgg tttttttcaa gcctattata gcagtctttt acccttaggc agcataaaaa
     9481 tacctaaaga tacgagtaac taatggacta ttttttgttt atttgctagt atgatagaat
     9541 acagaaagca aatctgtcat gcaagcttgg gatcatagct catcaggata gagcaactgc
     9601 cttctaagca gtaggtaggg ggttcgagtc cctctggtcc cgctcatata aagctgttca
     9661 aatgagcagc tttttttatt ttcgctcttt cattagttct ttgatttttt caaagctttc
     9721 atcacttata acatgctcaa tagcgcaggc atcgcattct gcaacatcag gagaaacacc
     9781 gagggatata aaaaaagcgg tgaggctttc gtgtttttct tttagttctt ggtatttttg
     9841 cttaccttgc tcggtgagct gaatggggcc gtagtgctcg tgctcgatca tacctcggtc
     9901 aacaaggttg tgcagggcgg cgtgtacgga gggctttgaa agtcccaaac gttttgctat
     9961 atcgattacc cgtggggttt tattttcttc ggataaaaag cctattgctt ctaaataatc
    10021 ttccaagctt tttgtcatca tttttacctc ataaaagatt taaggggtcg aggtctactt
    10081 caatgtaaac accagagata ggcttatagt gggaaagaaa tcgctcaacg gctgtatgta
    10141 tatattttaa ctcggtagca cgcagcagaa tttgatgtcg gtgattttgt gctatcatag
    10201 cgaggggggc aggggagggg ccgagaatct catcccagta ttcctcatcc tttggggcgt
    10261 ttttttgttt aagcgcttcg agttctgctt caagtatcgt ataggctgac tcggcggctt
    10321 ctgcagattt ttccttactc gctgagcgaa aaacaaggct tatgagtcgt gagaagggtg
    10381 gaaaaaagag gctttttctt tgttcgattt cccatttata aaagccttcg atatcggtat
    10441 tgcaggcaaa gtggattgct gggcgctctg ggtaaaaact ttggattata accttgccgt
    10501 ctgggaaaaa tcgccctgcc cgtcctgcaa cttgcaccaa aagggaaaaa acccgttcat
    10561 tggcacgaaa atcgggcatg tgtaagccgg tatccgctag gacaacgccg acgagtttta
    10621 agccagggaa gtttaagccc ttggcgacca tctgtgtccc aagcaaaata gatgtttttc
    10681 catttctaaa tcgttccagt ttttctgcaa ggtcgttttt gttttctaag ctgtctgtgt
    10741 cgagccgttc aatgctatag ccagggaatt ttgcttgcac ttctttttct ataaattcag
    10801 ttccaaagcc gtagtaggca atgtcgaggc tattgcaaga agggcaggaa ttttgcgctt
    10861 ccatcgtcca accgcaataa tgacagcgaa atcgtttttc gcttttatgg taggtgaggg
    10921 aaacagaaca atttttgcat tgcaaatctt taccacagga attgcactta aaaaaatgag
    10981 taaagccacg acggtttaaa aaaagcatta cttgtcgttc ttcttttaaa acttctcgca
    11041 tgttttcttc aagttcaggg cttatactcg agcaagacat gtcagtgcaa gaaagattta
    11101 caacttgcat ttctggtttt ttcccaccgc tcaatctttc tgtgagtttg tgctgtatta
    11161 ttattttttc tgacattgca tgccaagctt caagagaagg tgttgcactt cccataacaa
    11221 gaagggattg atgcctacta gagcgcatca tcgccacttg tcgcgcgtga taacgcggtg
    11281 tatttcctgc cttatatgaa gcgtcatgtt cttcatctat aataataagc ccaaggtttt
    11341 cgacaggtgc aaagatagcg cttctcgccc ctacaacaat ctttgccttc ccctgtataa
    11401 tttttttcca ttcagcaatt ttctgggcaa tagaaagggc cgaatgtaaa acagcaagtg
    11461 agtctccaaa acgttcgcgt atttcttcga ttacttgatg ggttagccca atttctggta
    11521 cgaggtagat gacagatttt ccctcggcta aagttttttt tgcgagctgt aaaaaaactt
    11581 ctgtttttcc cgttcctgta tgaccaaata aataatgaag taaggtattg ttttcttttc
    11641 cagcaaagat atcttctata gccctttctt gttcttgtga tagatttttt tctgcatagc
    11701 ttgcttcttc agtaaagcca gttgaaaagc ccatgccccc atcgctcgct cgtcttcccg
    11761 atggtatcat cgcactcaaa acttcaccat gcgcacaaag ataaaatcgc gcaatctttt
    11821 ttgataaatc gatgagttcg tcggtaaaga ctcgctcgct gtctatgatg cgaagaaggg
    11881 attttatttt ttcaagcggt actccgagat tgagggatgc gatttcttct tgccttactt
    11941 cttttaccga tataataaag cccgtcatct tacgatttcc aaaataagtt tcacagcgca
    12001 agcccgcttg aatttcaaaa ggtttttctc cctttatttt cggtagcttt gttgtctcag
    12061 aaaccgtata cgtaaatgat tgatttatag gcacattgaa aagtacctcc acaaaaatca
    12121 tttgtttttt ccttccatca tcagtatatc gtcgagaacc cttcgaacaa gttttaggtc
    12181 ttgccaggct ggtcgtttta aggcttcgtc atggagaagg gttaggggag aatagcttac
    12241 aagcaaggga gtgctttctt tttgtaggga tgaagcttta tagtcaaaaa ctttaccacg
    12301 aatatcttga aagttttgtt ttggaccaag gaggcagagg cttgtaaggc ttccaagcgc
    12361 aaggataaag tgcggttgca ttttttgtat ttgaatatct aaaaatgttt tacaagcata
    12421 catttgctca agcagtggta gtttattttc tggagggcgg catttaatta ttggcgtaat
    12481 ataggtattt gtatcttctg aaagtgaaat agcttgaagc atcttgctta aaagcctgcc
    12541 atcttcaccc gacataggta tgccgctttc atcatcctgc attgttgggg cttctgttat
    12601 aacgagcaca agtggatttt ttactccccg tccttcaatc gcttgttttc gagattgatg
    12661 caaggcgcaa cgagtacatt cttgcatctt tgcatagagc gaagatgaag tatcgatgtt
    12721 tgtcgtcctt gtgttttcct gaatatgccc ggaacctagg ttttcttgtg gtttttgtat
    12781 ccttggatat tctgtttttt gaaaatgctg cgggtaattt tttgtaaaac tcggtttttg
    12841 aggcattttt tctataaagc tatttaaatc atcttttact gtatagccaa gaaatgcaga
    12901 ctcaattgta ttgaggtttt cccagagttt ttgtgctttt tcaaaagata tcttgttcat
    12961 cactattaaa atactataca aagagaagct atgcaagggt aggctttgac taatcctttt
    13021 ttctctatta agataagcct atgaaacgat atcttttttt gttttattgt atcttacttg
    13081 tattctcact ttttgcccta aatgatggtc aaaggttagt gcctgcaggg cattgggtct
    13141 atgaaagtct atggaagcta agcttggaaa ctggcgaagt tcctcttttg aatacagcgc
    13201 ccgtgtcgag ggctgaaata gatcggtata tgacagcact cgatacagct cgtctttctg
    13261 tagcgggtgt gcaagagcga gagagagttc taggctttct aaacaaaggg actttttcgt
    13321 ataatccttc tccgtttact gtcggcatag atccgagtct tcatatagaa agctactata
    13381 agtcaagcac tgaaattccc tgggtttatg acaataaaga aaaaaaacac cttctagatt
    13441 tgccaattat catctctctc gaaaactatc ttacagctga aagtaatttt ttccttgcca
    13501 ataattactg gacaatgctt gaagcagata atgttctaaa tatccctctt ggcgatatgg
    13561 atccagacat tcatttccct gactttgcct atatttcttt tggccttcct ttttcgggca
    13621 atttttcttt taatgcacaa cttggtaagg gtgccctaag tcttggaaaa acgcaactgg
    13681 gaagcataat cttaaacgat agtttttatc acgatgccta tgctgttttt tccctctatt
    13741 cgccaagatt tcgatatgca gcctatacct tgcaagcaga agtaaataaa tatctgtata
    13801 tgcataaaat ggattttaat tttaaacgat ttcagttttc aatccttgaa ggagcaatgg
    13861 tagccgcacc tttagaacta cggtttttaa atccggtgat gatttttcac aattatgcag
    13921 cctggcgtag ttacgccgac tatgatgaag aacatgaaaa aatagaaagt cgagttggct
    13981 cttattttgc agcaagcttt gattataatc cttggaaaaa catgagactt tatggaatat
    14041 ttgcctttaa tcaattccag cttccctttg agcggaaaaa ttttggcgat gccgctgcat
    14101 ctattcccga cagccttggt tttcagggcg gttttgaaac aagccttccg tacaaggcag
    14161 gttattttgt tggtggcatc gaaggcgttt atacaagccc atggttttat tttactgaat
    14221 caaaagaaag ctccttctat agcaaacgtt tagacctcat aaaaaacacg agtaaaaata
    14281 taaatagctg ggtaggtttt ccctttggac cagacacaat tgctggaaat ttttactttg
    14341 gctatgacaa gggcggagat tggtctgcaa aaatctttta tcggttttta gctcagggag
    14401 aaatgtcggg cgaaaagggt tttgctattt ttgaaaatcc tggtttttat ccttcaaacc
    14461 caaaagaagc cttaatcaca agcccctctg gcatagcgca attttctcat acgataggta
    14521 taggaggttt gtataaacct ttgtcatatc tagaaatttc atcgcaacta gcctatactt
    14581 ttattataaa taaaaataat ataaaaaata ataatggctc gggagtagag cttgttttat
    14641 caacttcttt tctgcttccc taaaaaaagg actatacatg tttcaaaaaa aaatactttc
    14701 ttgccttgtt tttgttttgc ttttaacact cgtttttggg caagttgcga caagcccaaa
    14761 tgacccatta tacgatgata ttttaatatg ggaaaatctt ttactcatac caaatcaaag
    14821 ccctgttcgc ccctatcctc tagcgaccat caaaactatt ttagataggg taattgcctc
    14881 tagcaattca ttgcaagctt ctatcgcaga agccattacc agagaatctt tggaaaaccc
    14941 ctctcttttt ctcttggcta cgaaccacgt tttaaatttt cggacactgg agaaaggctt
    15001 gtgcaacaaa acatacttcc ggctgtagaa ggagattttt ttctaacaga aaagacttcg
    15061 cttgcctttg atttgggctt tcatgttatt gatgattatg ctgcttccta tagcaagcag
    15121 tttacaaatg attctatcct accgtttttt tctcgcccaa aacacgatgc acccgatgac
    15181 cctgcagaac ttgcctcttt ttctgccctc accaatatga atatgaatct tgcttatggt
    15241 ggagaaagat ggtatatgca gggtggtatg tcaaggctta gttttgggcc ctttcataca
    15301 gaaagcgtaa ccctttcgag cgaaggctat cattcaggaa accttatttt ttattat
//
LOCUS       DAOSSW010000038        15330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_3969_length_15330_cov_6.454534, whole genome shotgun sequence.
ACCESSION   DAOSSW010000038 DAOSSW010000000
VERSION     DAOSSW010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 15330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15330
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3969_length_15330_cov_6.454534"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PKW26_04680"
     CDS             <1..883
                     /locus_tag="PKW26_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ92893.1"
                     /translation="DSLLEKVSFLEASAKQTNPSSFDIHPSIKKIQDFLEKNEFSPSY
                     SSGITHKIRSEFSLDDLDNYAKVYERVLSWIADSILVEKPSYNTKPQIIMLVGPTGVG
                     KTTTIAKIAAHLTVLDTSQRKRAHIITIDKVRIAAIEQIEAYGKYMDIAVTVATSNND
                     LRNQVDKYASSVDYIFIDTVGASPNDEKLLLNMKDIVYLRENNNFITYLTLSASTKNS
                     DMKDIMKSYALFDYDSLIITKLDETKSVGNIISVLADTQKTLSYITTGQSVPRNIEIA
                     SVAKLVSLLEDEKQGTM"
     gene            903..1787
                     /locus_tag="PKW26_04685"
     CDS             903..1787
                     /locus_tag="PKW26_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOQ92894.1"
                     /translation="MEDQATELKEIMKQRGSGVQNSARPAGHKTRIIAITSGKGGVGK
                     TNIAVNMAIAYAQLGKKVILIDADLGMANVNVLLNIVPNANLLHVINQQRKMSEIVLE
                     TDYGIKFIAGANGFSRIANLNEKELQYFVEEFLTLSNADIIIIDTAAGISNNVLSFVA
                     AADEVFVITTPEPTAITDAYGIIKIIATELQNTNISMKLLVNRVQSADEGRKISDRII
                     NIAAQFLNHKVDYLGFIYDDPAVTASVIRQKPWLVASPSAKPSLCIKHIVGRIEKTDF
                     IEEEGLGRFLRRFRKKGR"
     gene            1859..2608
                     /locus_tag="PKW26_04690"
     CDS             1859..2608
                     /locus_tag="PKW26_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92895.1"
                     /translation="MQNPKVIGIFAGIGFVLSLLIGLFSGVKVSQLFLRAFMSALFSG
                     LLAWGLSFLLLKVFENVEELEAQQGDEAQKEVLGNYVNISLGDDSLEEEDNAPHFFVN
                     AKQMQSAPSVETASRVKTDQQDGDTFTKAELQKEGKGVQENKSFSEEKGKTVSEAVEE
                     KNSFQPRNLVEEAIHASKEEDTTEEAKSIAEELDDLPAISVISREMGANAEDVHDLGA
                     GTMQVERVDGAPQEDTSTIAQAIRTALAKDS"
     gene            2627..3415
                     /gene="whiG"
                     /locus_tag="PKW26_04695"
     CDS             2627..3415
                     /gene="whiG"
                     /locus_tag="PKW26_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HOQ92896.1"
                     /translation="MSDILEGQKTEEELWQEYKKTKSQKIRDIFVRQYMPLVKYVAGR
                     LAVNMPASVEYDDLVGFGQFGLLDAITKFDPDKNVKFKTYAVTRIRGSIYDELRQLDW
                     VPRSIRQKSKEIETAIMDVESRLGRQATDVEVATELGVSESEYHQTLLKVSGTSLLSL
                     NDTWFSSDGSDSLSIEDSIEGNPSLNPDVVVEREEIRRIITKSIHELPEKEKLVLILY
                     YHEDLTLKEIGAVLEVSESRISQLHASAKDKLRAKLTNVRKGIL"
     gene            3439..5415
                     /locus_tag="PKW26_04700"
     CDS             3439..5415
                     /locus_tag="PKW26_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOQ92897.1"
                     /translation="MVTLDMIRNDMEIDLEQNQAIRYVDVGADSIDEALSDAAVQLDS
                     RVSQLEYEILEKGNNGVLGFAKKPWLLRVYENEAATAKRKKESKKDSFFEDSFEEEVE
                     KIDRDGLFYIRYFGEDIHLKVLLPIGDGKPVDAKEILHRLQRSDTLSVNDSLVTKLVK
                     NGTEEKYIAIGKYKHSTAADALILVDIANDEMKASITVTPPAVGGSEVHADQIETLLM
                     NRGIVAGIDREKIEEFVDNPIYNVPCVVAEAIQPVDGRDAYIAYNFETDSSKLRFKEA
                     EDGRVDFKEHNLIQNVVEGQPLAQKMLAEQGKAGKTILGRYLEAKNGKDINLPLGKNV
                     IVDSDGRTILAATNGQVLLIGDKINVEPVMQIAGNVSIKTGNITFLGTVIVKGNVDDG
                     FNIKASGNIEVYGTVGKSILEADGDIVVSQGILGRDEGYVRAGRSLWAKFIQNTKVDV
                     EEYIIVAEGIVNSQVTSNKKILVQGKRASIMGGHVFATEEIYTKNLGSPGGGSETIIE
                     VGYDPRSKRRLTELQEKQAVLIRELDEIELNLQTLENTKKVRKTLPHDKEESLVKFTA
                     RKEEILVESEAMSAEIQQIQQYLRDLKVIGKVSAMGTVYAGVKIHVRDAKDDVRTEVK
                     AVTFFYEDGFVRRGKYEAPSDDDTKRVPDGYSSN"
     gene            5396..5713
                     /locus_tag="PKW26_04705"
     CDS             5396..5713
                     /locus_tag="PKW26_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92898.1"
                     /translation="MAILPIDLQTLYGQLEKVSKTAVQQQAAHLQSELHNEKEAHRLA
                     ARKEAVVETEKTEEGPNINKDGHSHSGGTGQGKKKESSEDEAQEKEIIKDPQLGKKID
                     VSG"
     gene            5717..6358
                     /locus_tag="PKW26_04710"
     CDS             5717..6358
                     /locus_tag="PKW26_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92899.1"
                     /translation="MTVYIALFTILNVSLWVLLFARLKKEFSPAAVLREVRQEVEKLI
                     IEINKEVDQDISLIEARRSGLKELLEKIDERILTIDKKIGLIDSQNALLREEEKILEK
                     LSPQRAYLKNKTKNQKADMPLFSQSKEKNTTESIFSLNQEEPERFLQGRVEKEKQEEP
                     IRVSFSKNPINQGKAMKDLVISMALQGKDESEIADALKMPVREVAFIIELHGN"
     gene            complement(6355..7146)
                     /locus_tag="PKW26_04715"
     CDS             complement(6355..7146)
                     /locus_tag="PKW26_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ92900.1"
                     /translation="MAKIEVKNLTAKYEKGKNVLENLSFTVEDGEILCLIGPNGGGKS
                     TLLTLLAGIGMGKIKKTKNSEILINQKKIESYAANKKAREISYLGQTEKLAWHFSVFE
                     TVLMGRYAYSSAYLPYSKEDKQIALSVLEDLGIFHLKDRLTNEISGGELQKVRIARSL
                     CQNTDIMLLDEPFSHLDIQHQSKLLSLIKTIAEEKKKTIVISLHDLNLCPLLSKTILL
                     VNKTEAFFGSLEEIFTESYLNKVYKTDFGLFRHPELDIPQVFVKN"
     gene            complement(7139..8137)
                     /locus_tag="PKW26_04720"
     CDS             complement(7139..8137)
                     /locus_tag="PKW26_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOQ92901.1"
                     /translation="MKKPQRANFSFYLAILTLSLIVLLLFFLSLILGSESISLGKIAT
                     AFKEKDYSSFVLTIILKIRLPRSILALLSGALLAASGTVFQGYFRNPLADPGLIGVSA
                     GATLGAVIFSSLSLFIPIGAFFGGLMAASFVYLIAGGSKTSTDSSRLLLSGTALGVLF
                     AAITSVLLILKDKEIYKMYLWTQGSFNAKGWNILFMVLFPSLLSFFLMFLTTKKLDLL
                     SFGEKSAETLGLNLQGTKLLCLISASLASASSVCAGGTIGFIGLIAPHTMRILFGSRH
                     KKLLPLSMLFGSSLVLFSDILARKIAPPMEVPIGIITAFIGVPFFLFLLRRGRQDG"
     gene            complement(8144..9028)
                     /locus_tag="PKW26_04725"
     CDS             complement(8144..9028)
                     /locus_tag="PKW26_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOQ92902.1"
                     /translation="MNLKNKKWISVSIVVLASALLCMGAFALGKKEGQNKAISRIVSL
                     SPAGTETLFALGLGDLLVARTDFCNYPPEALSVNSLGGFDGKTFSLESVLAFNPDLVY
                     LDVTMHGHLTDNLQNLGIQVFETNIASLDDIYKEIRELALLFDIKQRAEELITTIQRA
                     YTPMPLADMQTISLDTENEYAKVYWEVWNAPYMSVGSSSFINDVIREAGAINIFSDIA
                     AAYPVVSEESIIARNPSLILLPADSPENWESLSKRTGWQSIDAIQNKKVIKIDGDLIS
                     RPGPRIGEAIRAVQEALQ"
     gene            9122..10000
                     /locus_tag="PKW26_04730"
     CDS             9122..10000
                     /locus_tag="PKW26_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92903.1"
                     /translation="MKKILQFPLLFSILILSASFFWAEENTAEAVSYSFWEDASRVIA
                     IPKKKNLELSTASSRSIDSIDSDEAISQDLDFDETILHVLKSYYQWYYDGFYAQEKNL
                     NIVPLVRDNALYLRYWKRMEIEDAVFLYTPQSNIRDIAIIPAKIDAEVLAFILTDEAI
                     YPLRYWLTNESYDERKASVKLSETKTVFVDAYLSFGDVVYTSVTGKRNEVRNLRSFSR
                     NDAEKVGFYEKKSFDDGKYLVMSEPDFTALDLDSMLALQALIDTKNARRKPPRAPLIE
                     YQDLNFYYDVIEKLRR"
     gene            10159..11088
                     /gene="cysK"
                     /locus_tag="PKW26_04735"
     CDS             10159..11088
                     /gene="cysK"
                     /locus_tag="PKW26_04735"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOQ92904.1"
                     /translation="MNVAKNILALVGKTPLVSLSRWEKEKQLNAQIIAKLESYNPAGS
                     VKDRIAKAMIEDAFEKKRIDKNSVIIEPTSGNTGIGLAFVAAQYGLKIILTMPETMSI
                     ERRALLKAYGAEIVLTEGSKGMKGAIQRAEELAQEIPNAYIPGQFENPVNPLIHYETT
                     GAEIWEDSDGKVDILVSGVGTGGTLTGAGRYLKEKNPAIQVVAVEPSASPVLSGGEPG
                     AHKIQGLGAGFIPKTLDTEIYDSIIQVDNDDAFETGRELGRVEGLLCGISSGAALYAA
                     SRLAEDPKNAGKNIVVIFPDTGERYLSTPLFTS"
     gene            11223..13154
                     /locus_tag="PKW26_04740"
     CDS             11223..13154
                     /locus_tag="PKW26_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HOQ92905.1"
                     /translation="MKSYIDTKTKLSMGLDPFLPFASVSAKNSKSFQVDFEKEFASMI
                     LSASGWRKVFVSSGDEEDASESIGEVNAALAVYIAESFAKYIKEATGKASPRIVIGLD
                     ARPTGTEIGQILLQVLGHRDISLEYVFISAAPEIMAYAREKDGFVYVSASHNPVGHNG
                     IKFGLNDGGVIPGAESAKLTALFKDLCTREDAFSKAVELYESAQKTNIAALFSAVAKN
                     KEKALASYESFTKEVISGETEASKQDAFLDLLGKSAKKTALSILVDMNGSARAISIDK
                     SFISKLGFGFHAFNDSVRDIVHAIIPEPENLVHCAKKMEEMQKGGDSSVVLGYMPDCD
                     GDRGNIVYWDRKKKKAGILEAQEVFALSVLSELAFTALRHKAFTKEAIEKLSIGIAVN
                     DPTSMRIEELADAFGARVFRAEVGEANVVNLARAKREEGFLVPILGEGSNGGNITHPA
                     AVRDPLNTLFALLKLLSLRGTKDEPGLFKLWLSLSGQEAAYKDDFDLVDIINTLPQFT
                     TTGVSEERALLDIKTLDHAVLKKRYQSIFYAQWNEKKMALKEKWGIASWRAVANKGME
                     QKEAIADFSLSEKGGLKIIFSSAQKKDIAFIWMRGSGTEPVFRVMSDVKGSDVKAKEM
                     EKELLAWHSQMILLSDAED"
     gene            13264..13710
                     /locus_tag="PKW26_04745"
     CDS             13264..13710
                     /locus_tag="PKW26_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOQ92906.1"
                     /translation="MSVRGELFTTQVVLDNRSYFFNVKENRTGDVFLQVVESKNKDGS
                     NFDRHAIVVFEEDMQKFFKGLETSLQFIEKDKKEKKKKAAEKKAARQAYNEKMKPGSK
                     SLDASSKKIAMKKVQSKRSAKPSDTSDERKPDGIKRSGKVIVVKKS"
     gene            13707..15050
                     /locus_tag="PKW26_04750"
     CDS             13707..15050
                     /locus_tag="PKW26_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOQ92907.1"
                     /translation="MKTMTEGKPFSLILKFSLPLLAGNLLQQMYNLVDSIVVGHLVGE
                     NALAAVGSTNIINFLLVSLFSGLALGFTILVSQFVGAKEEAKVRRAVETAFISGIVGA
                     VLVTILGILLTKPLLQLLNSPEGQVRTMSETYLKILFIGTIGTFGFNLNAGILQGLGD
                     SISSLKFLAVATILNIILDLVFVVVFGWGVAGVAWATIIAQVVSFILGLLYLNKKLQL
                     FSFKILSWEFSNKIFIEALRIGLPGGVQNMLFSLGTMAIQRLINSYGAAYMAGFSISS
                     RIDSIAFLPIVSFASAATTFTGQNVGAKRLDRVAQGHKTVQMMSAFVVVCISILVVLF
                     APFLMAVFSPEQEVIQAGVEVLSRLMPCYVLVSILFIVNSVLRGAGESLMPFLSSMVS
                     FLIFRMPAAYILDYFFGRSEIGWSYGIGWVFGLIIILPYYFSGRWKKRIIQKYEK"
BASE COUNT         4923 a         2696 c         3356 g         4355 t
ORIGIN      
        1 tgatagtttg ttggaaaagg tgagtttttt agaagcatca gcaaagcaaa caaatccttc
       61 ttcatttgac atacatcctt cgattaaaaa aatacaagat tttttggaaa aaaacgaatt
      121 ttctccttcg tatagcagtg gaattacaca taaaattcgc tctgaatttt cgctcgacga
      181 cttggataat tatgcaaagg tctacgaacg agttctcagc tggattgctg attctatttt
      241 agtagaaaaa ccttcttaca atacaaaacc acaaatcatt atgctcgtgg gtcctactgg
      301 agtggggaaa actacaacta tcgcaaaaat tgcagcccac ttgactgttt tggacacctc
      361 gcaaagaaaa cgtgctcata ttattactat cgataaggtg cgtatcgctg ctatcgaaca
      421 gattgaagcc tacggcaaat acatggatat tgcggtaact gttgcaactt caaacaatga
      481 cttacgaaac caagtcgaca aatatgcttc aagcgtggat tatattttta tagatacagt
      541 cggggcgagc ccgaacgatg aaaaattact gctcaacatg aaagatattg tgtatttacg
      601 ggaaaataat aacttcatca cctatttaac actctcagct tcaacaaaaa atagcgatat
      661 gaaagatatt atgaagtcct atgccctatt tgactatgat tctttgatta tcacaaaact
      721 ggatgaaaca aaatctgttg gcaatattat ttcagtttta gctgatactc agaaaacact
      781 ctcctatatt acgacggggc aatcagttcc tcgaaatata gaaatagcga gtgtcgcaaa
      841 acttgtgtcg cttttagaag atgaaaaaca agggacaatg taaaaaagag gagaataaat
      901 atatggaaga tcaggctacg gaattaaaag agataatgaa acaaagagga agtggtgtac
      961 agaactcagc gcgccctgca gggcataaaa cgcgcattat tgctataaca agtggtaagg
     1021 gcggtgttgg caagacaaat attgcggtaa acatggcgat cgcctacgca caactgggaa
     1081 aaaaagttat cctcatagat gcggacttgg gtatggcaaa tgtaaacgtg cttttaaaca
     1141 ttgttccgaa tgcaaatcta ctgcatgtta taaaccagca aagaaaaatg agtgaaatag
     1201 ttttggagac tgactacggt ataaaattta ttgcaggtgc aaatggcttt tcacgcatag
     1261 caaatctaaa tgaaaaagag ctccagtatt ttgtagaaga attcttaacg ctttcaaatg
     1321 cagatataat tattattgat actgcagcag gtatttcaaa taatgttttg agcttcgtcg
     1381 ctgctgccga tgaggttttt gttataacca ccccagagcc aacagctatc accgatgcct
     1441 atggtattat aaaaattatt gcaacggaac tgcagaatac aaacatttcc atgaaattgt
     1501 tagtaaaccg ggtgcaatct gcagatgagg gtcgtaaaat atccgatcgc attatcaata
     1561 ttgccgcaca gtttttaaac cataaggtag attatctggg atttatttat gacgatccgg
     1621 cagtaaccgc ttcggtgatt cggcaaaaac cctggcttgt ggcaagtccg tctgcaaagc
     1681 caagtctttg cataaaacat atcgttggaa ggattgaaaa aacagatttt atcgaagaag
     1741 aaggcttagg acgctttttg agaaggttta gaaaaaaagg cagatagcat ttttaacttg
     1801 acctaatgca cattttgcct taatctattt atgttgagca ctttttttgg aggaatatat
     1861 gcagaaccct aaagttatcg gcatctttgc aggaattggt tttgtattat ccttgttgat
     1921 aggtttgttt tcaggcgtaa aagtcagtca actgtttttg cgagctttta tgtctgcact
     1981 attttcagga cttttggctt gggggcttag ttttttactt ttgaaagttt ttgaaaatgt
     2041 agaagagctt gaagcacaac agggtgatga ggcacaaaag gaagtgcttg ggaattatgt
     2101 aaatataagc ttgggagatg attctttaga agaagaagat aatgcgccac atttttttgt
     2161 taatgctaaa caaatgcaat cggcgccttc ggtagaaacc gcttcacgag taaaaacaga
     2221 tcaacaggac ggtgatactt ttactaaggc tgaacttcaa aaagaaggga aaggagtcca
     2281 ggaaaataaa agcttttctg aagaaaaagg gaaaactgtt tccgaagcgg ttgaagagaa
     2341 aaattctttt caaccacgaa atttggttga agaagctata catgcttcta aagaagaaga
     2401 tactacagaa gaagctaagt ctattgcaga agagcttgat gatttaccag ctatttctgt
     2461 gataagtaga gagatgggtg cgaatgcaga agatgtacat gatcttggag cggggactat
     2521 gcaggtagag cgcgtagacg gagctccaca agaagataca tcgacaattg cacaagccat
     2581 aagaacagcg ctagcaaagg atagttaaaa aaagggtgtg gtcataatgt cagatatact
     2641 tgaagggcag aaaacagaag aagaattgtg gcaagaatat aaaaaaacaa agtctcaaaa
     2701 aattcgcgat atttttgtac gtcaatatat gcccttggta aaatatgttg ctggaagact
     2761 cgctgtgaat atgccggcaa gtgtcgagta tgatgacctt gttggctttg gtcagttcgg
     2821 cttgcttgat gctataacaa agtttgatcc agataaaaat gtaaaattca aaacctatgc
     2881 agtcactcgt atacgaggtt ctatttacga cgagttgcga cagcttgact gggtgccacg
     2941 ttctattcga caaaaatcca aagaaattga aacagctata atggacgtag aatcacgcct
     3001 tgggaggcaa gcaactgatg tggaagttgc aacggaactc ggtgtatctg aaagtgaata
     3061 ccatcaaaca ttgctaaaag tttctggaac aagcttgctt tcattaaatg acacctggtt
     3121 ttcaagcgat ggaagcgatt ctctctccat cgaggatagt atagaaggga atccgagttt
     3181 aaatcctgat gttgttgttg aacgcgaaga aattcgtcgg attattacaa aatcaataca
     3241 tgaattacct gaaaaagaaa aactcgtcct catactctat tatcacgaag acttaacact
     3301 taaagaaata ggtgcggttc ttgaggtaag tgaatctcgc atatcgcaat tacacgcgag
     3361 tgcaaaagat aaattacgcg caaagcttac gaatgtgcgt aaaggtatat tataataaaa
     3421 aataaactag gagaaggcat ggttacatta gatatgattc gaaatgatat ggaaatcgat
     3481 ctcgaacaaa accaagccat ccgctatgta gacgtcgggg cagatagtat agatgaagcg
     3541 ctttcagatg ccgcagtgca acttgattca agagtctcac aactagaata cgaaatttta
     3601 gaaaagggca ataacggtgt tttaggtttt gcaaaaaaac cctggctttt gcgtgtatat
     3661 gaaaatgaag cagcaacagc gaagcgtaaa aaagagagca agaaagatag tttctttgaa
     3721 gatagctttg aagaagaagt tgaaaaaatt gatcgagatg gtctttttta tatccgctat
     3781 tttggggaag acattcatct caaagtgctt ttgcctatag gagatggtaa acctgtagat
     3841 gcaaaagaaa tactacatcg tttacaacgt agcgatacct tgtctgttaa cgactccttg
     3901 gttactaaac ttgtaaaaaa cggtactgaa gaaaaatata ttgcgatagg aaaatataaa
     3961 cattcaactg ctgctgatgc actcattctg gtagatattg caaacgatga aatgaaggca
     4021 agtattaccg ttactccacc agcggttgga ggatcagaag tacatgcaga tcaaatagaa
     4081 acgcttctta tgaaccgagg catagtcgct ggtatagata gagaaaaaat tgaagagttt
     4141 gtagacaatc caatctataa tgttccttgt gttgttgcag aggcaattca acccgtcgat
     4201 ggaagagatg cttatattgc ctataacttt gaaacagatt cttcaaagtt gcgttttaaa
     4261 gaagcagaag atggacgggt tgattttaag gaacacaatc tcattcaaaa tgtggtagaa
     4321 gggcaacctc ttgcacaaaa aatgcttgct gaacaaggaa aggcaggaaa aactatctta
     4381 ggtcggtatc ttgaagcaaa aaatggaaaa gacataaatc taccgctagg aaaaaatgtt
     4441 attgttgaca gtgatggaag aacaatttta gctgcgacga atgggcaagt ccttctcata
     4501 ggggataaaa taaatgtcga accagtaatg caaattgccg gaaatgtttc tattaaaaca
     4561 ggaaatataa cattcttggg aactgttatt gtcaagggca atgtagacga tggtttcaat
     4621 attaaggcat ccggaaatat cgaggtatac ggtaccgttg gaaaatcgat actggaagcc
     4681 gatggcgata ttgttgtttc acaaggtatt ttgggacgtg atgaaggtta tgtgcgtgca
     4741 ggtcgttcac tgtgggcaaa atttatacaa aatacaaagg ttgatgtaga agaatatatc
     4801 atagttgcag aaggtattgt aaactcacag gttacatcaa ataaaaaaat ccttgttcaa
     4861 ggaaagcgag catccattat gggcggacat gtatttgcaa ccgaggagat ttacaccaaa
     4921 aaccttggaa gcccaggtgg tggtagtgag acgattattg aagtcggtta cgatcctcga
     4981 tcaaaacgac gacttaccga attgcaagag aaacaggcag ttttaattcg agaattagat
     5041 gaaattgaat taaacttgca aacgctggaa aatacaaaaa aggttcgtaa aactttacca
     5101 cacgataaag aagagagctt agttaaattt actgcaagga aagaagagat tttagtagag
     5161 tccgaagcaa tgtctgctga aatacagcaa atacaacaat acttgcgaga tttaaaggtt
     5221 ataggaaaag tatcggccat gggtactgtc tatgctgggg taaagataca tgtgcgcgat
     5281 gcgaaagatg atgtgcgcac cgaagtaaaa gctgttacct ttttttatga agatggattt
     5341 gttcgacgag gcaaatacga agccccctcg gacgatgata ctaagcgggt accagatggc
     5401 tattcttcca attgatttac aaacgcttta tggtcaatta gaaaaagttt ccaagacagc
     5461 tgtgcaacag caggctgctc atttgcaatc ggaattgcat aatgaaaaag aagcgcatcg
     5521 acttgctgca aggaaagaag ctgtggttga aactgaaaag actgaagaag gtccaaatat
     5581 caacaaagat ggtcattctc attctggagg gacggggcag ggaaagaaaa aagaaagctc
     5641 agaagatgaa gcgcaagaaa aagaaataat taaggacccg cagcttggaa aaaaaattga
     5701 tgtttctgga taagctatga ctgtatatat tgcattattt acgattttaa acgtttctct
     5761 gtgggttctt ctctttgctc gccttaaaaa agagttttcg ccagctgcag ttttacgaga
     5821 agttcgacaa gaagttgaaa aacttataat tgaaataaat aaagaagtcg atcaagatat
     5881 tagtcttata gaagcacggc gctcaggatt aaaggaactg cttgaaaaaa tcgacgagcg
     5941 gattcttaca atcgataaaa aaattggact catagatagt caaaatgcgc tcttacgtga
     6001 agaggaaaaa atcttagaaa aactgagccc ccaacgcgcc tacctaaaaa ataaaaccaa
     6061 aaatcaaaaa gcagatatgc ctttattctc tcaatccaag gaaaaaaata ccacagaaag
     6121 catctttagt ttaaatcaag aagaacctga acgctttctt caaggtcgag ttgaaaaaga
     6181 aaagcaagaa gagcctatac gtgttagttt ttcaaaaaat cctatcaatc aaggaaaggc
     6241 aatgaaggac cttgttataa gtatggcgct tcagggaaag gatgagagtg aaatagcaga
     6301 tgccttaaaa atgcccgtgc gagaggttgc gtttattatt gaacttcacg gtaattaatt
     6361 tttgacaaaa acctgcggta tatctagctc gggatgccta aaaagaccaa agtctgtctt
     6421 gtacactttg tttaagtagc tttcagtaaa tatctcttcg agacttccaa aaaaagcttc
     6481 ggttttattg actaagagaa tcgtctttga taaaagtggg caaaggttca aatcgtgtag
     6541 gcttataaca atcgttttct ttttttcttc tgctattgtt tttatgaggg agagaagctt
     6601 gctttgatgt tgtatatcga ggtggctaaa gggctcatct aaaagcataa tatcggtatt
     6661 ttggcaaagg ctccgtgcga tacgtacctt ttgtagctcg cctccagaaa tttcatttgt
     6721 taagcgatct tttaagtgaa agattcctaa atcctcgagg acagaaagag ctatctgctt
     6781 atcttctttg ctatacggaa ggtaggctga ggagtatgcg tagcgtccca tgagcaccgt
     6841 ttcaaaaacg ctaaaatgcc aggcaagttt ttctgtttgc ccaaggtatg aaatctccct
     6901 tgctttttta tttgccgcgt agctttctat ctttttttga ttgatgagaa tttcagagtt
     6961 ttttgttttt tttatctttc ccatgcctat tccagccaag agcgtgagga gggtagattt
     7021 tcccccgcca tttggaccaa tgaggcagag aatttctcca tcttcgacag taaaagagag
     7081 attctctaaa acattttttc ctttttcata cttcgcagtg aggtttttta cttcgatttt
     7141 agccatcttg ccttcctctt cggagtaaaa acaaaaagaa gggcaccccg ataaaggcgg
     7201 taattatacc gatgggaact tccattgggg gggctatttt tcgtgcaaga atgtcagaaa
     7261 ataaaaccaa actgctacca aagagcatac tcaaggggag tagtttttta tgtctgctcc
     7321 caaaaagaat ccgcattgta tgtggtgcta tcaagccaat aaaaccgatg gtgccaccag
     7381 cacaaacgga gcttgcactt gcgagggatg cacttataag gcagagtaat tttgtcccct
     7441 ggagatttaa gccaagtgtt tcagcagatt tttctccaaa ggaaaggagg tcaagctttt
     7501 ttgtggtgag aaacataaga aaaaaggaaa gtaaactggg aaagagtacc ataaaaagaa
     7561 tattccatcc ttttgcatta aaacttcctt gcgtccataa atacatttta tagatttctt
     7621 tgtcttttag gatcaataaa acagatgtta ttgcggcaaa taggactccc aaggcggttc
     7681 ccgaaaggag caagcgcgag gagtccgtac ttgttttgct tcctcccgca ataaggtaaa
     7741 caaagcttgc tgccataagt cccccaaaaa aagcaccgat ggggataaaa agcgagaggc
     7801 ttgaaaatat aacggcgccg agggttgccc ctgccgaaac cccgatgaga ccggggtcgg
     7861 cgagcgggtt acgaaagtag ccttggaaaa ctgttccgct cgcagcaaga agcgctcctg
     7921 aaaggagggc taagatgctt cgagggaggc gaatttttag aatgattgtg agaacaaagg
     7981 aagaatagtc tttttcctta aatgctgtgg ctattttccc aagggaaata gactctgaac
     8041 ctaggatgag ggagagaaaa aaaagcagga gaactatgag gctcaaagtg agtatagcca
     8101 gatagaagct aaaattcgcc ctttgtggtt ttttcatttt attttactgg agcgcttctt
     8161 gcacagcacg aatcgcttcc ccaattcggg gacccggtcg tgaaataagg tcgccatcga
     8221 ttttgattac ttttttgttt tggatagcat cgatggactg ccaaccagtt cttttggata
     8281 gactttccca gttttcaggt gagtcagctg gaagcaggat gagggaaggg tttcgtgcaa
     8341 tgatgctctc ctcacttacc actggatagg cagctgcaat atctgaaaaa atatttatag
     8401 caccggcttc acgaataaca tcgttgataa aagagctgct tcccacactc atataggggg
     8461 cattccacac ttcccagtat acctttgcat actcgttttc cgtatcaagc gatattgttt
     8521 gcatatcggc aaggggcatg ggagtatagg ccctttgtat cgtggtgata agctcttcag
     8581 ctctttgttt tatatcaaat aaaagcgcga gttctcgtat ttctttgtaa atatcatcaa
     8641 gacttgcgat attggtttca aaaacctgta taccaaggtt ttgcaaatta tctgttaagt
     8701 gtccgtgcat tgttacgtcg agatagacaa ggtcgggatt aaaggcgaga actgactcaa
     8761 ggctaaaggt tttcccatca aaaccgccga ggctatttac actgagtgct tctggtggat
     8821 aattacaaaa atctgttcgt gcaacgagca aatctccgag accaagtgca aaaagcgttt
     8881 cagttccagc tggactgagg gaaacaatgc gagatatggc cttgttttgt ccttcttttt
     8941 tacccagagc aaaagctccc atgcaaagta gtgctgaggc aagcactacg atagatacag
     9001 atatccattt tttgtttttc aaattcataa ctgctcctct gtttttattt tttttggata
     9061 atggcatatc atacaaaaaa ataaatgttt tgtcatgaga aatctgccga tactagggta
     9121 aatgaaaaaa atcttacagt ttccacttct tttttctatt ctcattctgt ctgcatcctt
     9181 tttttgggca gaggaaaata cagcagaggc cgtttcctat agtttttggg aagatgcatc
     9241 tcgggtgatt gccattccca aaaagaaaaa tctcgaactt tccactgctt cttctcgctc
     9301 gatagatagc atagatagcg atgaggctat ttcacaggat ttggactttg atgaaactat
     9361 tttacatgtt ctcaaaagct attatcagtg gtattacgat ggtttttatg cacaggaaaa
     9421 aaatcttaac atagtcccct tggttagaga caatgcccta tatttacggt attggaaacg
     9481 aatggaaata gaagatgctg tttttttata tacaccacaa tcaaatatcc gcgatatcgc
     9541 tattattcca gcaaaaatag atgcagaagt ccttgctttt attcttacag atgaggctat
     9601 ctatcccttg aggtattggc ttacaaatga gtcctacgat gagagaaagg cctcggtaaa
     9661 attatcagaa acaaaaactg tttttgtcga tgcctatctt tcttttggcg atgtcgtcta
     9721 tacctctgtt accggaaagc gaaacgaggt gagaaatctt cgctccttta gccgaaatga
     9781 tgctgagaag gtggggtttt atgaaaaaaa aagcttcgat gatggaaaat accttgtgat
     9841 gagcgagcca gattttaccg cattggacct tgatagtatg cttgccttgc aagcactcat
     9901 cgatacaaaa aatgcccgaa gaaaacctcc ccgtgcacca ctcatagaat accaagactt
     9961 gaatttttat tacgatgtta tagaaaaatt acggcgctaa ggtaaaaata gttgttgttt
    10021 tattaatact atttatttca cttgacagaa atcatgattt catagtatgt ttttattacc
    10081 tagtaatcct atggggaata taaaaatgaa ttagagaatg ggatttataa aaacccaaaa
    10141 cgcagaggag gctcgtatat gaatgtagca aaaaatattt tggctttggt aggaaaaacg
    10201 cccttggttt cgctcagtcg atgggaaaaa gagaagcagt taaatgcgca gattattgca
    10261 aagcttgaaa gttataatcc agcaggttcg gtaaaagacc gcatcgcaaa agcaatgata
    10321 gaagatgcat ttgaaaaaaa acgcatcgat aaaaactcgg ttattattga gccaacgagt
    10381 ggcaatacag gcatcggact tgcctttgtt gctgcacagt atggtttgaa aattatcctt
    10441 actatgccag aaacgatgag catagaacgc cgggcgcttc taaaggccta tggtgcagaa
    10501 attgtactta cggagggaag caagggtatg aagggggcca ttcaaagggc tgaggagctc
    10561 gcccaagaga taccaaatgc ctatattcca gggcagtttg aaaatcctgt taacccgctc
    10621 attcactacg aaacaacggg agcagagata tgggaggata gcgatggaaa ggtcgatatc
    10681 ttggtctctg gtgttgggac gggcggaacg ctaacgggtg cagggcgcta tctaaaagag
    10741 aaaaatcctg ctattcaggt tgttgctgtg gaaccaagtg catctcccgt tctttctgga
    10801 ggagagccag gagcgcataa aattcaaggc ttgggcgcag gttttatccc caaaacgctt
    10861 gatacagaaa tctacgatag tattatacag gtagacaatg atgatgcctt tgaaacagga
    10921 cgcgaactag gaagggtaga aggactactc tgtggtattt ctagtggtgc tgccttgtat
    10981 gctgcaagtc gtcttgctga agacccaaaa aatgcaggca aaaacatcgt tgtgattttc
    11041 cccgatacgg gagagcgcta tctttcaacg cctttattta ctagctagaa tttacgggtt
    11101 tttctgcagt atattaaatt gtttggcaaa atactagact cgaccaaagc tttgtttttt
    11161 agtatattga ttgggagagt ctctgtttaa gctaagctaa attatgtaca aaggaataaa
    11221 cgatgaaatc atacattgat acaaaaacaa aactttctat gggcctagac ccatttttac
    11281 cttttgcttc agtatctgca aaaaacagca aatcctttca agtagacttt gaaaaagaat
    11341 ttgcttcaat gattctttct gcctcaggct ggcggaaggt ctttgtttca tcaggcgatg
    11401 aggaagatgc aagtgaaagt attggggaag taaatgctgc cctcgctgtc tatattgctg
    11461 aaagttttgc gaaatatata aaagaggcta cgggaaaggc aagtccgcgc attgttatcg
    11521 gcttagatgc tcgtcctact ggcacagaaa tagggcaaat cctcttacag gttttaggcc
    11581 atcgagatat aagcctcgaa tatgtgttta ttagtgctgc accagaaatt atggcttatg
    11641 cacgagaaaa agatggattt gtctatgttt ctgcgagcca taatccggtt ggtcacaacg
    11701 gcatcaagtt tggtttaaat gacggtggcg ttattccggg agctgaatct gcaaagctga
    11761 ccgcattgtt caaagacctc tgtacgagag aagacgcttt ttcaaaggcg gttgagctct
    11821 acgaaagcgc acaaaaaaca aatatagctg ctcttttttc cgcagttgca aaaaataaag
    11881 aaaaagccct cgcaagctat gagtctttta ccaaagaagt tataagcggt gaaacagaag
    11941 cttctaaaca agatgctttt ttagatttac tcgggaagag cgcaaaaaag acagcgcttt
    12001 ctatcctcgt agatatgaac gggagcgcgc gagctatctc tatcgataaa agctttatca
    12061 gcaagcttgg gtttggtttt cacgctttta atgatagtgt tcgagatatt gtacatgcaa
    12121 ttattccaga acctgaaaac cttgtacatt gtgcaaaaaa aatggaagaa atgcaaaagg
    12181 ggggagactc ctcagtagtc cttgggtata tgcccgattg cgatggagac aggggaaata
    12241 ttgtctactg ggatagaaaa aagaaaaaag ccggtattct cgaagcgcaa gaggtttttg
    12301 ccttatctgt tttgtctgag cttgccttta ctgcccttcg gcacaaggca tttacaaaag
    12361 aagcaataga aaaactctct attggtattg cggtaaacga ccccacttct atgcggatag
    12421 aagaacttgc cgatgccttt ggtgctcggg tatttcgagc agaagtaggt gaggcaaatg
    12481 tcgtaaatct tgctcgagct aaacgagaag aaggttttct cgtgcctatt ttgggagaag
    12541 gctcaaatgg aggaaatatt actcatccag ctgcagtgcg tgacccactc aatacccttt
    12601 ttgcgctttt aaaacttttg agtcttcgtg ggacaaagga cgaacctggt ctttttaaac
    12661 tctggctgag tctctcagga caagaggcag cctacaaaga cgattttgat ttagtcgata
    12721 ttatcaacac gcttccacag tttacgacta cgggtgtatc tgaagaacgg gccttgctcg
    12781 atataaaaac cctcgaccac gctgtactta aaaagcgcta tcagtctatt ttttatgcac
    12841 aatggaatga aaaaaagatg gctctcaaag aaaaatgggg tatcgcatct tggcgagcgg
    12901 tagcaaataa gggcatggaa caaaaagagg caatagcaga cttttcgctt tcagaaaaag
    12961 gtggactaaa aatcattttt tcatcagcac aaaaaaaaga tattgccttt atttggatgc
    13021 gtggtagcgg aactgaacca gtttttcgcg tgatgtccga tgtcaaaggc agtgatgtga
    13081 aagcaaaaga aatggaaaag gaattactcg cttggcatag tcaaatgatt ttactcagcg
    13141 atgcagaaga ctaggcatct gcacgcataa aaaagtagat attgcttatt cggataagaa
    13201 gtgatatatt tttttattag agaagtagtt agtagttaga gaaagaaaga taaaggtggc
    13261 ataatgagcg ttcgaggaga actttttaca acacaggttg tattggacaa tagatcctat
    13321 tttttcaatg taaaggaaaa tcgtacaggt gatgtatttc ttcaagttgt agaaagcaaa
    13381 aataaggacg gtagtaattt tgaccgccat gcaattgttg tttttgaaga agatatgcag
    13441 aagttcttta agggtttaga aacatctttg cagtttatag aaaaagacaa aaaagaaaag
    13501 aagaaaaaag ccgctgagaa aaaagcggct cgacaagcct ataacgaaaa aatgaaacca
    13561 ggtagcaagt ctttagatgc gagttccaaa aaaatagcta tgaaaaaagt ccagtcgaag
    13621 cgttccgcaa agccaagtga tacaagtgat gagcgtaaac ctgatggcat taagcgatct
    13681 ggcaaggtaa ttgttgtaaa aaaatcatga aaacaatgac agagggaaaa cctttttccc
    13741 ttatccttaa attctcacta cccttacttg caggcaatct tttacaacaa atgtacaacc
    13801 ttgtcgattc cattgtcgtt ggtcatttgg taggagaaaa tgcacttgct gcggtcggca
    13861 gcacaaatat cataaatttt ttactcgttt cgcttttttc ggggcttgca ctcggcttta
    13921 ctattcttgt ttcacaattt gtgggtgcaa aagaagaggc aaaggttcgg cgtgcggtag
    13981 aaacagcctt tatttcaggc attgtcggtg cggttttggt taccatcctt ggaattcttc
    14041 taacaaagcc actcttgcag ctactcaata gtcccgaagg ccaggttcgc acaatgtctg
    14101 aaacctacct aaaaatcctt tttattggaa caataggtac cttcggcttt aatctaaatg
    14161 caggcatcct gcaaggctta ggcgattcta tttcatctct caaatttctt gctgttgcaa
    14221 ctatactcaa tattatactc gatcttgttt ttgttgttgt ctttggctgg ggtgtagcag
    14281 gtgttgcttg ggcgaccatt attgcacaag tggtttcttt tattttagga cttttatatc
    14341 tcaataaaaa actgcaatta tttagcttca aaattcttag ttgggaattt agcaataaaa
    14401 tttttataga agcacttcga attggtttgc ctggaggcgt gcaaaatatg cttttctccc
    14461 tcggcacaat ggcaattcag cggcttataa atagctatgg agcggcatat atggcaggtt
    14521 ttagtatatc gagtcgtatt gattcaattg cctttttacc cattgttagt tttgcaagtg
    14581 ctgcaacgac ctttacgggg caaaatgtcg gcgctaaacg gctcgatcgt gtcgcacaag
    14641 gacataaaac cgtacaaatg atgtcagcct ttgtcgttgt atgtatatct attttagttg
    14701 tcctttttgc tccctttttg atggctgttt tttcaccaga acaagaagtc attcaagccg
    14761 gagtagaggt cctttctcga ttgatgcctt gctatgtttt agtatcgata ttatttattg
    14821 taaattctgt tttacgcggc gcgggagaat ctctcatgcc cttcctttct tctatggtat
    14881 catttttgat ttttagaatg cctgcggcat atatcctcga ttattttttt ggacgaagcg
    14941 aaataggctg gtcctatggt ataggctggg ttttcggctt aatcattatt ttgccatatt
    15001 atttttcagg aagatggaaa aaaagaatta tacaaaaata tgaaaaataa actgctaaat
    15061 atgataaaag cccttgacct ttttttatat atttagtata tttaatgtac tgacgatgag
    15121 aagtacaaaa ttgtaaacct catcaagacg aaatggtgtt tatcatcata accgagtgaa
    15181 ggaaatggtt gcttttactt ttttacaagt ttttgcgctg ttactaaaag ctttattaga
    15241 aagacatatc gcttgatatt ttttcttaaa aaaacaaaaa aagattgata gagctcttga
    15301 ctaagtttta gagctgtgtt actctcttcg
//
LOCUS       DAOSSW010000039        15196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_4003_length_15196_cov_7.632719, whole genome shotgun sequence.
ACCESSION   DAOSSW010000039 DAOSSW010000000
VERSION     DAOSSW010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 15196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15196
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4003_length_15196_cov_7.632719"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKW26_04755"
     CDS             <1..329
                     /locus_tag="PKW26_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92908.1"
                     /translation="RKNISLSAFIFSGIYPCPGAVLVLILSFTLNIIKLGITSVLVMS
                     LGMSIPILISAYIAWAGREGLFYFFKNKEGIIAKLTFFIELFGYILLLVFSFYIAWPF
                     LIGLLR"
     gene            complement(343..1380)
                     /locus_tag="PKW26_04760"
     CDS             complement(343..1380)
                     /locus_tag="PKW26_04760"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR00675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOQ92909.1"
                     /translation="MKLISLFSGAGGLDLGFTQAGFTISLANEFDNTIWETYEKNHGH
                     ILLKKDIRDISQDDLPLNIDGLIGGPPCQSWSEAGALKGIEDDRGKLFYEYIRILNYV
                     KPKFFLAENVSGMLANRHSDAVKNILSLFKSCGYDVDLTMVNASDYGVPQDRKRVFYI
                     GFRSDLNIQFQFPEPTTKDKKRTLREAIGDLEAKEIAALPKNKTNGKLKIQNHEFFTG
                     AFSTIFMSRNRVRSWDEPAFTVQASGRQCQLHPQAPKMLKEEKDRHIFVPGKEHLYRR
                     LTIRECARVQTFPDDFEFIYSNLQDGYKMVGNAVPVELARIIALSIKHHLEVKTNATS
                     SISNIKKVVGI"
     gene            complement(1377..2240)
                     /locus_tag="PKW26_04765"
     CDS             complement(1377..2240)
                     /locus_tag="PKW26_04765"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HOQ92910.1"
                     /translation="MNNILTAIINISKSTKMTLRHKPTGNNRVQNMGEALEKYIQDVF
                     SNTMEVSDQITRTALLSNTFSYLGNANNPPDMMLLGGDAIEVKKIQSPKSAIALNSSY
                     PKAKLFASSHMISKACRESEVWEKKDMIYAIGVVHEHAISSLAFVYGEDYCAENATYE
                     KIKKTIKDGVESIPNVAFSESKELGHVNRVDPLGITYLRIRGMWGIENPFSVFDYVYQ
                     RNMDHAFNFMCIINDEKFNSFENTIDLYEECKKNKRLHISDIRIKDANNPARLKSAKL
                     ITYYIPEQEAT"
     gene            complement(2303..3556)
                     /locus_tag="PKW26_04770"
     CDS             complement(2303..3556)
                     /locus_tag="PKW26_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOQ92911.1"
                     /translation="MHTTKNNRSEKTEKMMKTYSIDFTKEDFKCRAKKLADLLLKKDS
                     SFKLALIIDSASLYYLTGTMQEGIALLFADGSLKYFVRRSYERALDESPLEDAIQPMT
                     SYRDLVPYLPENLEAVYVEKEVLPLVSFERLQKYLPAKKWLALDSFMARLRSVKDAKE
                     IALIEKAGSLHNQLLTKEVPRLLKEGMSEAELGMAIFQKAVEIGHYGMMKMQMFQVDF
                     LAGQIAFGENALYPMRFDGPGGMKGLHPATPILGSDERKLQKGDLVFVDLGFCYKGYL
                     SDKTQMYFFGDAIPEKVKAVHEACRHVLDEAVKEMKAGNTTKAVWEKAEAARGPLLQE
                     HFMGFGKGQVSFLGHGLGLKIDEAPVIMEKGESILEEGMVIALEPKSGIAGHGLVGVE
                     ESYVIEKNGARCLTGGDSEIILVEE"
     gene            complement(3662..4204)
                     /locus_tag="PKW26_04775"
     CDS             complement(3662..4204)
                     /locus_tag="PKW26_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOQ92912.1"
                     /translation="MKDLKGTKTEANLWEAFAGESQAHTKYQYYASVAKKAGFNQVAG
                     FFEETSRNEKEHAKIWFKLLHDGKVPATEVNLVDAADGENYEWTDMYAGFAKTAKEEG
                     FTKIAYLFEAVGKIEKHHEERYRELLASIKAGNVFNAEGEVAWICANCGHIHYGKEAP
                     ELCPVCDHPQGYFMRNVKNY"
     gene            complement(4251..5849)
                     /gene="lysS"
                     /locus_tag="PKW26_04780"
     CDS             complement(4251..5849)
                     /gene="lysS"
                     /locus_tag="PKW26_04780"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOQ92913.1"
                     /translation="MSKEKTILNHWADQTAEKIIRERGDLDLYTCASGITPSGTVHIG
                     NFREIISVELVVRALRDRGKKVRFIYSWDDYDVFRKVPENMPKKELLEEYLRFPITMV
                     PDTTERDNSYARHHEVDVENVLPKVGIYPEFLYQAEKYRNHDYVEGMKTALQNKEEIK
                     ELLNTYRDDAHKMKAEEAYWPVAAFCASCNKDTTEIRSYDGEWLLDYHCTSCGYEAKA
                     DIRSAKELKLGWRVDWPMRWAYEKTLFEPAGKDHHSQGGSFDTARLVSEKIYNWPAPV
                     SFRYDFIGIKGTPGKMSSSKGKVVDLYDVLEVYQPELVRYLFAGTRPNTEFSISFDLD
                     VIKTYEDYDKTERIAWGVEKAKNEQVYAKERRVYELSQVDGMPSIMPYQMGFRHLCNL
                     LQTASGDIDAVIANLTDIKEEQIGRFKERCICAWNWIRECSPEDFRFSLRSNGEKIAL
                     EDAELKALQRVAEEVLPQVEVLEEKPLSQLLYDIAKDEGIEPKALFTVLYQALIEKDQ
                     GPRLASFMKIIGKEKLQTLLSIYT"
     gene            6044..6415
                     /locus_tag="PKW26_04785"
     CDS             6044..6415
                     /locus_tag="PKW26_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92914.1"
                     /translation="MKKLVLLLVILLSAGLLFAADIDWDKLPKGKWLDENWNAVWEIG
                     GNSIRILDKTSGEEVFNFQDLISDFDYGASLTEAQFSFRCDEAERSYVFKKGFTNLDL
                     TMEIKADWTSETYVVKMPFQK"
     gene            complement(6480..7160)
                     /locus_tag="PKW26_04790"
     CDS             complement(6480..7160)
                     /locus_tag="PKW26_04790"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HOQ92915.1"
                     /translation="MKCKNCKIAFFSRTERCPLCGTEGELSHFDLQNDCYFYPEYALP
                     KSSKLDIAKKILFFLTIAFSSIAIFVNIFSLDIQGSLWSLLLASCLFYTFISFKTIFS
                     KEMHTGSKILIQLLLLSALAIIIDIFTGFRRWSTSFVVPSLSIVSSLVIVFVAAGRRS
                     LYKEFSGYLLTSFLVSIIPALLCLIKLSTHVWVGYAAFLVSLLMLLGLYVFSEKDFKN
                     EMKKRFHF"
     gene            complement(7150..8442)
                     /locus_tag="PKW26_04795"
     CDS             complement(7150..8442)
                     /locus_tag="PKW26_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92916.1"
                     /translation="MDKWYKLDNAARLYPHVANSNRTSVFRVSCVLHRTVNPVLLQEA
                     LDSIRKKYEFFFVRIHRGFFWNYFDINPKKLLVQSESETPCQDINKIKNAGFSLRVLY
                     YNKRVSVEIFHALSDGGGILEFLKSLVYYYLELEGFPIPNDGSIISKEGLPELDASDD
                     SFFRYYDDLKGLSKKERLIEKQKKAYKLQGSVFPSGGIQVTSLLLSSKALYALSKKYN
                     TTITGYLLALLFHALFETEVKYKRTKGPVSIAVPVNLRRAFPSKTLRNFFSVINIVLP
                     YTADDGLQELCQKVDEVLKEKTQKQYLQKQVRRTTRFSMTRWMRFAPLIIKKIFIQAG
                     FSLMSETKRTLTLSNLGIVSIPEQMLQHISHMEVVSHLTPRCPISCSMVSTGDTTCIS
                     FSKSISDMELVHFFTNYLSQKEGLDITVYANEWGNYEM"
     gene            8579..12025
                     /gene="dnaE"
                     /locus_tag="PKW26_04800"
     CDS             8579..12025
                     /gene="dnaE"
                     /locus_tag="PKW26_04800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOQ92917.1"
                     /translation="MSSFVHLHVHTDFSLLDGAASISQLVERAKELNMRSLAITDHGN
                     MFGVIHFQQACINNGIKPIIGCEFYVAGASRFEKSGTEQGNKYYHLVLLAKNEIGYRN
                     LMVLSSKAYLEGMYYKPRIDEELLEAYSEGLICLSACIAGELPQLLLNGQTKDAEAFV
                     ERYAKLFGKDHFYIELQDHGIADQKKVAPLLIDIAKKHDIPMVVTNDVHYTRKENHVA
                     HDILLCVGTKKLQKDMNRMRFDTEEFYLKSEEEMARLFPSYPEMIKNTEKVAAMCNLD
                     IPQYCVTELPDCLPVYEIPQGFTDEKAFFNHLVYSGLEKRYGKISDEIKQKADHEISV
                     ITSMGFIGYFLIVWDFINWAKENDIPVGPGRGSGAGSIVAYAMTITDIDPIRFNLIFE
                     RFLNPERISMPDFDIDICFERRQEVIEYLRNKYGEEQVGQIITFGKMKAKAVIKDVGR
                     VLDIPLSDVNKITKHIASSGKLKDSFSINPKVSDSGQLAPFMNEPSYKELFENSLVLE
                     GTNRNSSIHASGIVIGKTKLIDWVPMYKDSRTPIMATQYTMDIIEACGLVKMDILGLK
                     TLTLIKHTERLIHKRKGFENFDANIVDENDEKTFDLFCEGKTAAIFQFESPGMQKVLK
                     QAQPRRIEDIVALNALYRPGPMQFIDDFVAGKNDPTKIHYPDASLKDILEETYGVIVY
                     QEQVMQVAQKIAGYSLGQADNLRRAMGKKKPEVMAAEKENFVKGAVQSGFDAKKAEEI
                     FEILIPFAGYGFNKSHAAAYSVLAYRTAYLKTHFPLEFIAANLTNEMSSTDKLPEYIA
                     EAKSMGLEVKVPNINSSDKVFDVVDGAIQFGLLGIKGLGEIAADELIQERQKNGPYKS
                     FMDFLERVNLSAINKRALEVLIKTGCFDTLGERRSKLLLNLEQAVDYVSKKKEATEFG
                     QVSLFEDSGEKEFLDFTYEETEDWPELEKLRLEKELIGFYISGHPLEMHKKIIDSLVS
                     LRTNAIEHLSKERNYAILALVKEVRPILTKKQQWMGLGQLEDLHGTIKFTAFPKTWEQ
                     IKDLMEVDAILFFKGKYDDSYGGPTLLIDEVVLPERLESYVTKAVHIKLKDKTKDQTK
                     LMAFRDFIYSHPGSCEVYFHLETMRGDYEIKAPQSTSISCDDDFITELKNQDLVESIW
                     KE"
     gene            12029..12622
                     /locus_tag="PKW26_04805"
     CDS             12029..12622
                     /locus_tag="PKW26_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOQ92918.1"
                     /translation="MDLFFGLLRLLSFAISIYSFLCLIRLLLSFSPSLQQNSFYAFLT
                     SICDPYLYLFRKLTFLRLNQLDFSPIISLGLLYMLTQMLNTIIAIKRFSLGILLSVIV
                     NFAFSIVNSFLFFLIIILAIRLIFLLLNKDTSSIWFSFDQFIKPIADFITKKFYKNSF
                     YTFQKSLIISLISFIVFSAILKIVAFILVSFFQTLPF"
     gene            12634..14916
                     /gene="recG"
                     /locus_tag="PKW26_04810"
     CDS             12634..14916
                     /gene="recG"
                     /locus_tag="PKW26_04810"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOQ92919.1"
                     /translation="MKLEDLQTPITQVPGVGIKTERLFKQLNIFTVSQLLSHYPRDYE
                     DRTEKLCIKDFHVGKKVHTIAKVISHEWFGFGRMKTLKIRIQDTTGEAVLLCFNRPFL
                     EKSFPVGSIISVTGNFNYKYGEIQASFFEAKLLQHARENPFLEASPEPENFKEILPIY
                     PLTEGLKNAQVKTIIEAALKSYSLGLENEIPQALIDERGLLSKKEALEKIHNPKTIQD
                     IEKARYTLIYEELFLFQYHLLQAAHARKKNQPFTSRTSDSHAAYDRNSKNKTEVLDDT
                     HFAQFLSPKQKILLERIPFSLSSDQKKSILDINEDLEKAYAPNQGKNKNDSQGIINNS
                     FYMARLLQGDVGSGKTLVAFFSALYCADMGYQSAFLAPTELLAKQHAQNASHLLQGIP
                     GINIAFLSGNIKSQGRSLLLQSLASGDITIIIGTHALFSTNVKYHSLGFIIIDEQHRF
                     GVVQRASLLDKGRESIQKTPSYLMMSATPIPQSLALSAFGDLDISTIKTMPKGRGTIQ
                     THLTKVGNEWRVYEAVRKEIEKKRQAYFVYPRIEKKEDEDFLDSIFSDEDFPEKEKPN
                     LAAQKDFFENPNLKSAQEKKLKSAEEMYDFLSREVYPNYKLALIHSKIDEEEQSAIMH
                     AFKKGEIDILIATTVVEVGVDVPNATSMVIEHAERFGLAALHQLRGRIGRGSADSHCF
                     LIYAEKLSDTAKERLKAMYENLDGFKIAELDLELRGPGEVAGIQQSGNLQLGIADLVR
                     DRKILLEAREDAEKILKTKS"
     gene            complement(14952..>15196)
                     /locus_tag="PKW26_04815"
     CDS             complement(14952..>15196)
                     /locus_tag="PKW26_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOQ92920.1"
                     /translation="VKQGKPHPDIFLLAAKNLGVDAENCLVLEDSNFGLQAGQSAGMK
                     TLFIKDLVDPPMEVLAKVWNSASDLLEARDLIKELL"
BASE COUNT         4737 a         3179 c         2667 g         4613 t
ORIGIN      
        1 gtagaaaaaa catttccctt agcgcattta ttttttctgg catctacccc tgccccggtg
       61 cagtgcttgt tttaatttta tcttttacat taaatataat aaaactcggc ataacttcag
      121 tcctcgtcat gtccttgggt atgagcatcc ccattctcat ttcagcctac attgcctggg
      181 caggacgaga aggactcttt tattttttca aaaacaaaga aggcattatt gcaaagctca
      241 ccttttttat agaacttttt ggctatatcc tacttcttgt cttttcattt tatattgcct
      301 ggcctttttt aatcggctta ttgcggtaga cggcgggata acttatatac caacaacttt
      361 ttttatattg cttatgctag aagttgcatt cgtttttact tctagatgat gttttatact
      421 cagtgcaatt attctagcta gttctacagg aacagcattc cccaccattt tatatccatc
      481 ctgcagattt gaatatataa attcaaaatc atcaggaaag gtttgcactc gtgcacattc
      541 tcgtattgtc agtcttctat acagatgctc tttccctgga acaaagatat gtctgtcttt
      601 ttcttcttta agcatttttg gggcttgtgg gtgtaattgg cattgtcttc ctgaggcttg
      661 cacagtaaat gcaggctcgt cccaacttcg cacacgattt ctactcataa aaattgttga
      721 aaaggctcct gtaaaaaatt catggttttg tatttttagt tttccatttg ttttattttt
      781 tgggagagct gctatttcct tagcttcaag gtcgcctatc gcttctctca gggtacgttt
      841 tttatccttt gttgtgggtt cgggaaattg aaattggata tttagatcgc ttcgaaaacc
      901 aatataaaaa actcgctttc tgtcttgtgg aacaccatag tcagaggcat tgaccattgt
      961 aagatctaca tcataaccac atgacttgaa taaactaaga atatttttta ccgcatctga
     1021 atgtctattt gcaagcatgc cagatacatt ttcagcaagg aaaaactttg gttttacata
     1081 atttaaaatc ctaatgtatt cataaaaaag ttttcctcta tcatcttcta taccctttaa
     1141 tgcacctgct tcgctccaac tttgacaggg cggtccccct atcaaaccgt caatatttaa
     1201 aggcaaatcg tcttgcgaaa tatcacgaat atcttttttt aacaatatgt gcccatggtt
     1261 tttttcgtag gtttcccaaa tagtattgtc aaattcattc gcaagcgaaa tagtaaatcc
     1321 tgcctgagta aaacctaaat ctaagccacc agctcctgaa aataaactta taagtttcat
     1381 gttgcctcct gttctggtat atagtatgtt attaatttag cagatttcaa acgagctgga
     1441 ttatttgcat cttttatacg tatatctgaa atatgcaatc ttttgttttt tttacattct
     1501 tcgtataaat caattgtgtt ttcaaatgaa ttaaattttt catcattgat gatgcacata
     1561 aaattaaacg catggtccat atttctttga tacacataat caaaaacaga aaaaggattt
     1621 tcaattcccc acattcctct aatacgtaaa taggttatac cgagaggatc aactctatta
     1681 acatgaccaa gttctttact ttcagaaaaa gctacatttg gaattgattc tactccatct
     1741 ttaatagttt ttttaatttt ttcatatgta gcattctctg cgcagtaatc ttctccgtac
     1801 acaaaggcaa gcgaactaat tgcatgttca tgcacaacac caatagcata aatcatatct
     1861 tttttttccc atacttcgct ttctcgacaa gctttactta tcatatggct acttgcaaat
     1921 aattttgctt ttggataact actatttaaa gcaatagctg attttgggct ttgtattttt
     1981 ttaacttcaa ttgcatctcc tcccagaagc atcatatcag gaggattgtt agcatttcct
     2041 aagtacgaaa aagtgttaga aagtaaagct gtacgagtta tttggtcaga aacttccata
     2101 gtgttagaaa aaacatcttg aatatatttt tctaaagctt cacccatatt ttgtacacga
     2161 ttatttcctg taggtttatg cctaagggtc atttttgtag attttgaaat atttatgatt
     2221 gcagttaaaa tattattcat tttaattacc tcaacctatt ctaacataaa agctagcaaa
     2281 cttctacttt tccccccccc tactactctt ccactaaaat tatctcgcta tcgccgcctg
     2341 tcaaacagcg agcgccgttt ttttcaatga catagctttc ttctactccc acgagtccat
     2401 gccccgcaat accggacttt ggctccaggg ctataaccat gccttcttct aaaatgctct
     2461 ctcccttttc cataataacg ggcgcctcat ctatttttaa gccaaggcca tgtcccaaaa
     2521 aagaaacctg tcctttccca aagcccataa aatgctcttg caaaagggga ccgcgtgcag
     2581 cttccgcctt ttcccaaaca gcctttgttg tattgcccgc cttcatttct tttaccgctt
     2641 cgtctagcac atggcgacaa gcttcgtgta ccgcctttac tttttctggt atcgcatctc
     2701 caaaaaaata catttgggtt ttatcactga gatagccctt ataacaaaaa ccgaggtcaa
     2761 caaagacgag gtctcctttt tgcaattttc gctcatcgct tcccaaaatt ggtgttgcag
     2821 gatgtaagcc cttcatgcca cctggtccat caaagcgcat tggatacaag gcattttccc
     2881 caaaagctat ttgccccgca agaaaatcaa cttgaaacat ttgcatcttc atcatgccat
     2941 agtgccctat ctccacagcc ttttggaaaa tagccatgcc gagttctgcc tccgacatgc
     3001 cctctttcaa aaggcgaggc acttcttttg tcaaaagttg attgtgtaag cttcccgctt
     3061 tttctatgag cgcaatttct tttgcatcct ttacacttcg caggcgagcc ataaaagaat
     3121 cgagcgcaag ccactttttt gcaggaaggt atttttgtag gcgctcaaag ctcacaagcg
     3181 gcagtacttc tttttctaca taaaccgctt ctagattttc aggcaaatac ggaactaagt
     3241 ccctatacga agtcatcggc tgtatagcat cttccaaggg agattcatcg agcgctcgct
     3301 catagcttct tcgtacaaag tattttagac tgccgtctgc aaataaaagt gctatgcctt
     3361 cttgcatcgt accagtcaaa taatacaaac tcgcagaatc aataataaga gcgagtttaa
     3421 aactgctatc ttttttcaaa agaagatcgg caagtttttt tgcacgacac ttaaaatctt
     3481 ctttggtaaa atcaatcgag tatgttttca tcattttttc ggttttctcg cttctgttat
     3541 tttttgttgt gtgcatacga gtatcttaac atagattatc aatcgagcct atttatttaa
     3601 atctaataga tactaataga caaaaaaaag actccatcaa atggagcctt ttaaaactag
     3661 cttagtagtt ctttacattt ctcataaagt agccttgtgg atgatcacaa acagggcaga
     3721 gctctggtgc ttctttaccg taatgaatat gaccacagtt tgcacaaatc catgcaactt
     3781 cgccttcagc attgaataca tttcctgcct taatgcttgc gagcaattct ctatagcgct
     3841 cttcgtggtg tttttcaatt tttcctacag cttcaaaaag ataggctatt tttgtaaaac
     3901 cttcttcttt tgcggttttt gcaaaaccag catacatatc tgtccactca tagttttcac
     3961 catcggcagc atctacgagg ttaacttcgg ttgcaggaac tttgccatcg tgcaaaagtt
     4021 taaaccaaat ttttgcatgt tctttttcat ttcttgatgt ttcttcaaaa aatcctgcaa
     4081 cttggttaaa tccagctttt tttgcaactg aagcatagta ttgatatttt gtatgcgctt
     4141 ggctttcacc cgcaaatgct tcccataaat tggcttcggt ttttgttccc ttaagatcct
     4201 tcatttttta ctcctataaa aaatatttaa acagtttcaa acaaaactgc ttatgtataa
     4261 attgataaca atgtctgtaa tttttctttt ccaataatct tcataaaact tgcaagtcgt
     4321 ggtccctggt ctttttctat caatgcttga tacaagactg taaacaaggc cttcggttct
     4381 atgccctcat cctttgctat gtcatacaaa agctgcgaaa gcggtttttc ttcaagaact
     4441 tctacttgcg gaagaacttc ttcggcaacg cgttgcagcg cctttagttc tgcatcttca
     4501 agtgcaattt tttctccatt acttcgaaga ctaaagcgaa aatcttcagg agagcattct
     4561 cgtatccagt tccaggcaca aatgcaacgc tctttgaacc gccctatctg ctcttctttt
     4621 atatcggtga gatttgcaat caccgcatct atatcgccag aagcggtttg taaaagattg
     4681 cataaatgcc gaaaacccat ttggtagggc ataatcgagg gcatgccgtc aacttgtgaa
     4741 agctcgtaga cccgtctttc ttttgcataa acttgctcgt tttttgcttt ttcgacaccc
     4801 caggcaatac gctcagtttt gtcatagtcc tcataggttt ttataacatc taagtcaaag
     4861 cttatagaaa attctgtatt tggtcgtgtc cctgcaaaaa gatagcgaac gagctctggt
     4921 tgataaactt ctagaacatc gtataagtca acaacttttc cttttgaaga agacattttt
     4981 ccaggagttc cttttatacc gataaaatcg tagcgaaaac tcaccggagc aggccaatta
     5041 tatatttttt cactgaccaa tcgagcggtg tcaaaggagc caccttgtga gtgatggtct
     5101 ttccctgctg gttcaaagag ggttttttca taggcccatc gcattggcca gtcaactcgc
     5161 cagccgagtt tcaattcctt tgcactgcga atatctgcct ttgcctcata gccgcaactt
     5221 gtacaatgat agtctaaaag ccactcccca tcgtaggagc gaatttcagt tgtatcctta
     5281 ttgcatgaag cacaaaaagc cgcaaccggc caataggctt cctcagcttt catcttatgt
     5341 gcatcatcac ggtaggtatt taagagctct tttatctctt ctttattttg tagggccgtt
     5401 ttcatccctt caacatagtc gtggtttctg tatttttctg cttgatataa aaactctgga
     5461 taaatgccaa cctttggcaa aacgttttcc acatccacct cgtgatggcg tgcgtaagag
     5521 ttatctcgtt ctgttgtatc ggggaccatc gttataggaa aacgcaagta ctcttcgagg
     5581 agctcttttt tcggcatatt ttctggaacc tttcgaaaca catcgtagtc gtcccaagaa
     5641 taaataaaac gcaccttttt tccacggtcg cgaagtgcac gaacaaccaa ttctacagaa
     5701 ataatttctc gaaaattacc tatatgtacc gttccagacg gggttattcc agaggcgcag
     5761 gtatagagat ctaagtcccc acgctcacgt attatttttt cggcagtttg atccgcccag
     5821 tgatttaaaa tagtcttttc tttgctcata ttaaataaat ataccgttta tttacatatt
     5881 atacaagcat tcttaatgct taagcctatt ctgtgactat gatacttgtc tttctaaaaa
     5941 aatcactata tactagactc acatttattt cactttttca tataggaaat agatgaaaga
     6001 tgcgacaaaa tgtcgcctca gattcagatt cttaggagga tgtatgaaaa aactagtgct
     6061 actcttagtt attttactta gcgcagggct tttatttgcc gcagacattg attgggacaa
     6121 gcttccaaag ggaaaatggc tcgatgaaaa ctggaatgcg gtttgggaaa tcggtggaaa
     6181 ctcaattcgt attttggaca aaacaagcgg tgaagaagtg tttaactttc aagaccttat
     6241 ctcagacttt gactatggtg caagcttaac tgaagcacaa ttttctttcc gatgtgatga
     6301 ggcagaacgc tcctatgtat tcaagaaagg ttttacaaac ttagacctca ccatggaaat
     6361 aaaagcggac tggacaagcg aaacttatgt agttaaaatg ccctttcaaa aataaataaa
     6421 aagtaaatac tctacagaaa accgcctttt tcttggcggt ttttttattt taaaagcgct
     6481 taaaagtgaa agcgtttttt catttcattt ttaaaatctt tttcagaaaa aacataaagc
     6541 cccagtagca ttaaaagaga gacaagaaag gctgcatagc caacccatac atgggtcgaa
     6601 agttttatca agcaaagaag ggctggtata attgaaacga gaaaagaagt taaaagataa
     6661 cctgaaaatt ctttatacaa actccgtctt cctgctgcaa caaaaacaat aaccaaggac
     6721 gaaacaattg aaagcgaggg aacgacaaag cttgttgacc atcttctaaa gccagtgaaa
     6781 atatctataa ttattgccag ggccgaaagg agcaaaagct gaatgaggat ttttgaacct
     6841 gtgtgcattt cctttgaaaa aatagtttta aagcttataa aggtatagaa caaacaactt
     6901 gcaagcaaaa gtgaccatag agaaccttgt atatcgagtg aaaagatatt tacaaagata
     6961 gcaatactgc taaaggctat ggtcaaaaag aaaagtatct tctttgctat atcgagtttt
     7021 gagcttttag gaagtgcata ttctggataa aaatagcaat cattttgcaa gtcaaaatgg
     7081 cttagctctc cttctgttcc acaaagaggg caacgttctg ttcttgaaaa aaaagctatc
     7141 ttacaatttt tacatttcat aatttcccca ctcatttgca tacacggtaa tatccaatcc
     7201 ttctttttga ctgagataat ttgtaaaaaa atgcacgagt tccatatccg aaatggactt
     7261 agaaaaacta atacaggtgg tatcaccagt acttaccatg ctgcaggaaa tcgggcaacg
     7321 aggtgtaaga tgtgaaacaa cttccatgtg cgatatatgc tgtagcatct gttctggaat
     7381 agaaacaatg ccgaggtttg aaagcgtcaa tgtcctttta gtttcgctca tcaaagaaaa
     7441 acctgcctgt ataaatattt tttttattat caagggagca aagcgcatcc aacgagtcat
     7501 ggaaaaacgt gttgttctac gcacttgttt ttgcaaatac tgtttttgtg ttttttcttt
     7561 cagcacctca tcaaccttct gacagagttc ttgcaagccg tcatcagcgg tatatggaag
     7621 cacgatattt ataacagaga aaaaattacg aagtgttttt gagggaaagg ctcgtcgcaa
     7681 atttaccgga accgcaatgc tcacagggcc ttttgttctt ttgtatttta cctcggtttc
     7741 aaaaagtgca tgaaaaagta atgcgagtaa ataaccggtg atagtcgtgt tgtatttttt
     7801 tgagagagca tacaaggctt tagaagaaag taaaaggctg gtaacctgta tgccaccact
     7861 aggaaaaacg cttccctgta acttatatgc ttttttttgt ttttctatga gccgctcttt
     7921 ttttgaaagc ccttttaagt catcataata gcgaaaaaaa ctatcatcac ttgcgtcaag
     7981 ttcgggcaaa ccttctttag aaataatcga gccatcattt ggaattggaa atccttcaag
     8041 ctctagataa taatacacaa ggcttttcaa aaactctaaa atccctccac catcggagag
     8101 tgcatgaaaa atctcaactg aaacacgctt attataatac aaaacacgta agctaaaacc
     8161 tgcatttttt atcttgttga tatcttgaca gggtgtttcg ctttcgcttt ggacaaggag
     8221 ttttttaggg tttatatcaa aataattcca aaaaaaacca cgatggatgc gcacaaagaa
     8281 aaattcgtat ttttttcgaa tactatctaa ggcttcttgc aagagcacag gatttactgt
     8341 tctatgcaaa acacaagaaa cccgaaaaac actcgttcgg ttactattgg ctacatgcgg
     8401 atagagacgt gctgcattat ctaatttata ccatttatcc atgcctaaac ctcgctacta
     8461 tcataacttc tttttcactt taaagatagg gaactttttt ctattctttc catgcaaaaa
     8521 cagaatttaa gactttcaaa gattgataaa agaagcgctt agggttatac tagttaatat
     8581 gtcttcattt gttcatttac acgttcacac agatttttcc ttactcgatg gcgctgcatc
     8641 tatctcgcag ctggtagaaa gggcaaaaga gctcaatatg cgctcgcttg cgataactga
     8701 ccacggcaat atgtttggag ttattcattt tcaacaagca tgtattaata atggcataaa
     8761 acccattatc ggatgcgaat tctatgttgc cggagcatcg cgctttgaaa aatccgggac
     8821 agagcaagga aacaaatact atcaccttgt gcttttggca aaaaacgaaa tcggctacag
     8881 aaatctcatg gtgctttcct caaaagccta tcttgaggga atgtactaca aaccgcgtat
     8941 agatgaagaa cttttagaag catattctga gggacttatt tgtttgtcag cctgtatcgc
     9001 tggggaatta ccccaattac ttctaaatgg acaaacaaag gatgccgaag cctttgtcga
     9061 gcgctatgca aagctttttg gtaaagacca tttttatata gaattgcaag accatggcat
     9121 tgcagatcaa aaaaaagtag ctcccctgct catcgatatt gcaaaaaaac acgatattcc
     9181 catggttgtt accaatgatg tgcactacac aagaaaagaa aatcatgttg cacacgatat
     9241 tttactctgt gtcggtacaa aaaagctcca aaaagatatg aatagaatgc gctttgacac
     9301 cgaggaattt tacttaaaaa gcgaagagga gatggcaagg ctttttcctt cttatcctga
     9361 aatgataaaa aatacagaaa aagtcgctgc tatgtgcaat ctagacatac cgcagtattg
     9421 tgttaccgag cttcccgatt gtttacctgt ctatgaaatc ccacaaggct ttactgacga
     9481 aaaagctttt ttcaatcacc tcgtctatag cggcttagaa aaacgatatg gcaaaataag
     9541 cgatgaaata aaacaaaaag ctgaccatga aatttcagtt atcacctcga tgggctttat
     9601 cggttatttt ttaattgttt gggactttat taactgggca aaagaaaatg atattcctgt
     9661 cggtccaggg cgtggttctg gtgcgggctc catcgttgcc tatgcgatga caattaccga
     9721 cattgacccc attcgattta atctcatctt tgagcgcttt ctcaatcccg agcgaattag
     9781 catgcctgac ttcgacatag atatttgctt tgaacgacgt caagaagtta tcgaatatct
     9841 tcgcaataaa tacggcgagg aacaagttgg gcaaattatc acctttggaa aaatgaaggc
     9901 caaagctgtt ataaaagatg tcggccgcgt tttagatata ccactttcag atgtaaataa
     9961 aattacaaaa cacattgcat catcgggaaa actcaaagac agttttagca tcaatcccaa
    10021 agtaagtgat tctggacaac ttgctccttt tatgaacgag ccaagctaca aagagctttt
    10081 tgaaaactcc ctcgtccttg aaggcacaaa tagaaactca agcattcacg catctggaat
    10141 cgttatcgga aaaacaaagc tcatcgactg ggtgccaatg tacaaagact cgcgcactcc
    10201 cataatggca acacaatata ccatggatat tatagaagca tgtgggcttg tcaaaatgga
    10261 tatactcggc ttaaaaaccc ttacgcttat caaacataca gaacgattga tacacaagcg
    10321 aaaaggtttt gaaaacttcg acgcgaatat cgtcgatgaa aacgacgaaa aaacctttga
    10381 ccttttttgc gaaggaaaaa ctgctgccat ttttcagttt gaaagtcccg ggatgcaaaa
    10441 ggtcttaaag caagcacaac caagacgtat cgaagacatc gttgctttaa acgccttgta
    10501 ccgacctggt cccatgcagt ttatcgacga ctttgttgcc gggaaaaacg accccacaaa
    10561 aatacactat cctgatgctt ctttaaaaga tattctcgaa gaaacctatg gcgtcattgt
    10621 ctaccaagaa caggttatgc aagttgctca aaaaatagct ggctatagtc ttggacaggc
    10681 tgataatctg cgacgtgcaa tgggaaagaa aaaaccagaa gtaatggccg cagaaaaaga
    10741 aaactttgta aaaggtgctg tacagtctgg ctttgatgca aaaaaagccg aagagatttt
    10801 tgaaatcctc ataccctttg ccggctacgg ttttaacaaa agtcacgccg cagcctactc
    10861 cgtgcttgcc tacagaaccg cctacctcaa aacacatttc ccactcgaat ttattgcggc
    10921 aaacctcacg aacgaaatgt cttctacgga caagctcccc gaatatattg ccgaggcaaa
    10981 aagcatgggg cttgaagtta aggtgccaaa tataaacagc tcagacaagg tctttgacgt
    11041 ggttgatggg gcgattcagt ttggcttact cggtatcaag ggcttaggag aaatagcagc
    11101 tgacgagctc attcaggaaa gacaaaaaaa cggcccctat aaaagtttta tggattttct
    11161 cgaacgcgta aatctttctg ccataaacaa acgagccctt gaagtgctca taaaaactgg
    11221 ttgctttgat acgcttggag aaagacgatc aaaactgctt ttaaatcttg aacaggcagt
    11281 cgactatgta agcaagaaaa aagaagcaac agaatttggg caggtaagtt tatttgaaga
    11341 ttctggtgaa aaagaatttt tagactttac ctacgaagaa acagaggact ggcctgaact
    11401 tgaaaaacta cgccttgaaa aagaacttat cggattttat atttctggac atcccttaga
    11461 aatgcataaa aaaataatag actcacttgt ctcccttcgc acaaatgcca tagaacattt
    11521 gagcaaggaa cgaaactatg ctatcctcgc ccttgtaaaa gaagtgcgcc ccatccttac
    11581 aaaaaaacaa caatggatgg gactcggtca actggaagac ttacacggaa caataaagtt
    11641 tactgcattc ccaaaaacat gggaacaaat aaaagactta atggaagtcg atgcaatttt
    11701 atttttcaaa ggcaagtacg acgatagcta cggcggacca actttgctca tcgatgaagt
    11761 tgttttgcca gaaaggcttg aaagctatgt aacaaaagcg gttcatatta aactaaaaga
    11821 taaaacaaag gatcaaacaa aacttatggc ctttagggat tttatctatt cacacccagg
    11881 gagttgcgaa gtatactttc acttagaaac catgcggggt gattacgaaa ttaaggcacc
    11941 acaaagtaca agcatctctt gtgatgatga ctttataaca gaactaaaaa atcaagactt
    12001 agttgagtct atttggaagg aataaaagat ggatttattt tttggattat tacgattact
    12061 ctcttttgcg atttcgatct actcgtttct ctgcctcatt cggctcttat tgagctttag
    12121 cccgagctta cagcaaaaca gtttttacgc ttttttaact tctatctgcg acccctacct
    12181 ttatcttttt cgcaagctca cttttttaag gcttaaccaa ctcgatttct ctcccattat
    12241 ttctttgggg ctcttgtaca tgctcacaca aatgctaaac actatcattg caataaaacg
    12301 atttagcctt gggattttac tctccgttat agtaaacttt gctttttcta ttgtaaactc
    12361 atttcttttt ttcctcatca ttatacttgc gattcgcctc atctttcttt tactcaataa
    12421 agatacgtca agcatttggt ttagctttga tcaatttata aaaccaattg ccgattttat
    12481 tacaaaaaaa ttctataaaa attcttttta tacatttcaa aaatcgctta ttattagcct
    12541 tataagcttt attgtttttt ctgcaattct taaaatagta gcattcatac ttgtttcctt
    12601 tttccaaacg ctgccctttt aacttagctg cctatgaaac tggaagacct acaaactcct
    12661 atcacgcaag tccccggtgt cggaataaaa acagaaagac tttttaaaca actcaatatc
    12721 tttaccgtct cgcaattact cagccactat cctcgcgact atgaagacag aacagaaaaa
    12781 ctttgcataa aagattttca tgtaggaaaa aaagtccaca caattgcaaa agttatctcg
    12841 catgaatggt ttggctttgg gcgcatgaag acactcaaaa tacgcatcca agatactacg
    12901 ggtgaagctg tactgctttg ttttaatcgc cccttcttag aaaagtcatt tcctgttggc
    12961 tcgattataa gcgtcacagg aaactttaat tataaatacg gcgaaattca agcttctttt
    13021 tttgaagcaa agctccttca acacgcaaga gaaaatcctt ttctcgaagc aagtccagag
    13081 ccagaaaact tcaaagaaat cctccccatc tatccgctga ccgagggatt gaaaaatgca
    13141 caagtaaaaa caattataga agctgcctta aaaagttata gcctcggttt agaaaatgaa
    13201 ataccgcaag ctctcatcga cgagcgaggg cttttaagta aaaaagaagc cttagaaaaa
    13261 attcacaatc caaaaacaat acaagatata gaaaaggcaa gatacacgct catctatgaa
    13321 gagctgtttt tatttcagta tcatttattg caagctgccc atgcacgcaa aaaaaatcaa
    13381 ccctttacct caaggacaag cgactctcat gcagcctacg atagaaattc aaaaaataaa
    13441 acagaagtac ttgatgatac tcactttgcc caatttcttt ctccaaagca aaaaattctt
    13501 ttagaacgaa ttcctttttc gctgagcagc gatcaaaaaa aaagtatcct cgacatcaat
    13561 gaggacttag aaaaagccta cgctccaaat cagggaaaaa ataaaaacga ttcccaaggt
    13621 ataataaata attcttttta catggcacga cttttacaag gagatgttgg atcaggaaaa
    13681 acccttgtcg cttttttctc tgccctctac tgtgccgaca tgggctatca atctgctttt
    13741 ttggctccaa ctgaattact tgcaaaacaa catgcgcaaa acgcttcaca tttactccaa
    13801 ggcattcccg gtatcaatat agcctttctt agcggaaata taaaaagtca agggcgctca
    13861 ttattgttgc aaagccttgc aagcggcgac attacgatta tcatcgggac acatgcctta
    13921 ttttcaacaa atgtaaaata ccactcactt ggttttatca ttatagatga acaacatagg
    13981 tttggcgttg tgcaacgagc aagtcttttg gacaaaggaa gagaatccat tcaaaaaacg
    14041 ccatcctatc tcatgatgtc tgcaactccc ataccgcaaa gcctcgccct ttctgccttt
    14101 ggcgaccttg acatttcgac aataaaaaca atgccaaagg gacgcggtac aatacaaacc
    14161 caccttacaa aagtaggaaa tgagtggagg gtctacgaag cggtccgaaa agaaatagaa
    14221 aaaaaaagac aggcatattt tgtatatccc cgcatagaaa aaaaagaaga cgaagatttt
    14281 ttagatagca ttttttcaga tgaagatttt cccgaaaaag aaaaaccaaa cctcgctgca
    14341 caaaaagatt tttttgaaaa cccaaaccta aaaagcgcac aagaaaaaaa actgaaaagc
    14401 gcagaagaaa tgtacgattt tctctcgcgc gaggtttacc caaattacaa gctcgccctc
    14461 atccactcaa aaattgacga agaagaacaa agcgctatta tgcacgcttt taaaaaagga
    14521 gaaatcgata ttctcatcgc aacaactgtt gttgaagtag gtgtcgatgt ccccaatgca
    14581 acatctatgg tcatcgaaca tgcagagcga ttcgggctcg ctgctctcca ccaattgcgc
    14641 ggacgcatcg gccgagggag cgcagactca cattgttttt taatctatgc ggaaaagcta
    14701 agcgacaccg caaaagagcg actcaaggct atgtacgaaa atctagatgg ttttaagatc
    14761 gcagaactcg acctagagct tcgaggccct ggagaagttg ccggcattca acaatcggga
    14821 aaccttcaac tcggtatcgc tgatttagtt agagatagaa aaattctttt agaagcacga
    14881 gaagatgcag aaaagattct gaaaacaaaa tcctagaacc aaaaatgaac ataaagcttg
    14941 aagaaacaat tctacaaaag ttctttaatt aagtccctcg cttctaacaa atcacttgca
    15001 gaattccaca ccttcgccag cacttccata ggtggatcga caaggtcttt tataaacaag
    15061 gttttcatac cagcactttg accagcttgc aaaccaaaat ttgaatcttc taagacaaga
    15121 cagttttcgg catcaactcc taggtttttt gcggcaagaa gaaagatatc gggatggggt
    15181 tttccttgct ttacct
//
LOCUS       DAOSSW010000040        15072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_4048_length_15072_cov_5.847440, whole genome shotgun sequence.
ACCESSION   DAOSSW010000040 DAOSSW010000000
VERSION     DAOSSW010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4048_length_15072_cov_5.847440"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1335)
                     /locus_tag="PKW26_04820"
     CDS             complement(124..1335)
                     /locus_tag="PKW26_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HOQ92921.1"
                     /translation="MRITVKNNVQWVGKIDWELQRFHGDEYSTHAGSSYNSFLIQEEK
                     NVLIDTVWLPYAKEFVENVKEAIPLEKIDYVIMNHGEIDHSGALCELMSHIPDVPIYC
                     TEAAVKSLTGQYHQNWNFKTVKTGDSLDIGNGKKLVFVEMKMLHWPDSMATYLTGDNI
                     LFSNDAFGQHMASHELFNDKVDKDALYHECIKYYANILTPFSMLVHKKIEEVLALKLP
                     IDIIAPSHGVIWRENPAQIVEKYLQWSQDYAENQITILYDTMWEGTRKVAEAISEGLG
                     DKPCTGKKCTIKLMNLAKNDKNDVLTEVFKSKMILVGSPTIGRGLTHATAGILEMMEG
                     LRFKGKKAAGFGCYGWSGEGPAKITEALKRSGFEILDEGHKVLWNPDSDASEKAFAWG
                     KSLREKLDAAN"
     assembly_gap    1555..1564
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1565..2373
                     /locus_tag="PKW26_04825"
     CDS             <1565..2373
                     /locus_tag="PKW26_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOQ92922.1"
                     /translation="EYETYMVKDTRRAELLFEKYDDAICFVNIDTGFSEPEWETWIRK
                     ILAEPKFSKIGIGIVSYNSNEKLQKKYLMDIGIQCGYIKLKLGRDESIKILLATLNAN
                     EAKGRRKYVRADATGDALSSLNFTHGRLQIIGKIVDISVVGFACILDPDPELAKNMLL
                     SDVQLKLRASLLRTDLILLGSRPVDDRNQYIFLFPPKIENHAKAKIRTYIQTTLQASL
                     EQYLEEALKNAPKEAPRSSPQNTSPLLEPALEELEDLAEVEAIDEAAEKP"
     gene            complement(2410..>3264)
                     /locus_tag="PKW26_04830"
     CDS             complement(2410..>3264)
                     /locus_tag="PKW26_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOQ92923.1"
                     /translation="FPADEVGNHLVDLDALDEKGINPWSDVLSEADFRRSFGFPGHRF
                     TGIDYIAYYKSIVEAEDCEVEIVLANDPRAILKYTKNVLACDVHTRKRTMRLLKNAGG
                     EQVYGLEDCLKKPVGKSGYNEDFGLLGSNKATEESVKLFPRNCAKVVESIQEQIFEKT
                     GKKVEVMVYGDGAFKDPIGGIWELADPVVSPAYTGGLEGQPHEIKLKYLADNEFASLS
                     GEALKEAISKSIKEKSETDSQDMASQGTTPRRLVDLVGSLSDLTSGSGDKGTPIVYIQ
                     GYFDNFTS"
     assembly_gap    3265..3364
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3585..5702)
                     /locus_tag="PKW26_04835"
     CDS             complement(3585..5702)
                     /locus_tag="PKW26_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOQ92924.1"
                     /translation="MLKPMRPVIKVIEENCVNCHRCVAVCPAKMCNDGSGDYVELHSD
                     LCLGCGACIEACTHGARIGIDDAEVFFEALAKKEKIVAIVAPAAAASFNGKILQLNGW
                     LESLGVEGFFDVSFGAELTVKSYLEYKKDANPSCIIAQPCPTLVSFVEIYRPELIPLL
                     APCDSPMAHVMKMIKEFYPKFKNHKILVVSPCFSKKREFDDIGLGDYNVAFTSFEKYI
                     KDHDVKISDYPERDFDSPMAERAVLFSTPGGLMRTAGREVPGIFEKTRKIEGHPQVYH
                     YLAHLGDAIEKKEAPIYELVDCLNCEMGCNGGPGTSNKGKHPDIVEGMIERRSEEYKE
                     KIRSKALLKNPKRAQKKFNAVLNKYWRKGLYDRTYVDRSGVFKDMVRNPSNAEIERVY
                     KDMHKTKPQDILNCGACGYNSCEQMAVAIVNGLNRAENCRHYMSVEVAMLHDSHKQEI
                     NEVIQSISGESIKRLEQNMKDISFLADGSTEMANFVVESSAAIQQMVANVDSINRILG
                     DNASAVLELEEASKSGGEGLDMIVSLIEQISNQSEGMIEASAVIQQIASQTNLLAMNA
                     AIEAAHAGNYGKGFAVVADEIRKLAENAGSQASSISRVLDQLKTLIDEVSGSSEEALK
                     KFGQVLNLSEAVRNQGLVIKQAADEQSVGGQQVLDALTQVNELTTHVKDQAVALLSSS
                     QVLLEDISSLAKMEEYTAAEVIQ"
     gene            5863..6270
                     /locus_tag="PKW26_04840"
                     /pseudo
     CDS             5863..6270
                     /locus_tag="PKW26_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706761.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            6265..6729
                     /locus_tag="PKW26_04845"
     CDS             6265..6729
                     /locus_tag="PKW26_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOQ92925.1"
                     /translation="MIEKKQTKESGEGGYKWLVYAILGAVFASLTAILGKIGIQDIDS
                     NLGTAIRTIVVLIMAWLIIIGQGKVGQLKHIDKKSWIFIILSGFATGLSWLSYYRALQ
                     TGPASVVVPIDKLSIVLSVAFSYFILKEKLKKKSFLGLALIVAGTLTLIFFK"
     gene            6817..8631
                     /locus_tag="PKW26_04850"
     CDS             6817..8631
                     /locus_tag="PKW26_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOQ92926.1"
                     /translation="MKIAQKLLLIITGALITFLVANVIIVGSQFTQLSEDITIEDVAS
                     KLRSNINAAHLFLEDTYEGIILKNGSMVGTNGKNVENNTEFVDFLKSTFSTQATIFKR
                     EGGDFTRVATSILQDDGQRAVGTTLNENNIIYQKLTDGETYVGETRILDNPYIAIYSP
                     ILGQFGEVIGLLFLGENKETIQSMIRSYQVKGFLIVVSSSILAILIIVAFSYLLIKKT
                     ILHPITNTVSLLKNISEGDGDLSQRIEIQSKDEIGDLAKYFNLTVEKIGQTILLVKNQ
                     TTQLKLSGELLATNTNQTATAINQITANISSIQNQTVNQSASVTETSATIEQVSKGIE
                     RLNELITDQSANITQSSSAIEEMMANIHSVTMSLVKNEANISRLTESSQSGKTQVEKI
                     ANAITDVAKDSQSLLEVSSLIQGIASQTNLLAMNAAIEAAHAGEFGKGFAVVADEVRK
                     LAETSSTQTKTITAMLKKITASIQEITAYSDNVVSTFMLMEDEVKTVGTHEATILNAM
                     KEQNEGGRQILEAISSLNTITQNVHQNSEEMLTGSQQVLEETKNMNHITQEITDGMNE
                     MASGSKEISVAVEQVNQLSVENTLSIENLAKEVQKFKV"
     gene            complement(8647..9870)
                     /locus_tag="PKW26_04855"
     CDS             complement(8647..9870)
                     /locus_tag="PKW26_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOQ92927.1"
                     /translation="MNLKQKYAALLVRQGVNIQKGQTFVLSCPIEVADFGRLLQEEAY
                     KAGAREVVMRWIDEKSSKISYDMADDAIFDEVAPWVSSFYNGYVDQKAAFMTIVSDDP
                     ELMKDVDPKRIARAVKARSIALEHFRNEQMSGRNAWCVAAASSEGWARKVFPKDEPEV
                     AVKKLWDAIYKATRVDCEDPFLEWEKHQENFERRTKILNDYAFTHLEYKNSLGTDLRV
                     ALAKGHLWLGGSKDSETGYPFNANMPTEEVFTAPDAKGTDGRIYSSIPLNYQGNLIDK
                     FWFEIEDGFVVDFGAEKGAENLKQLLETDEGAKRLGEVALVSYDSPISNTGILFYNTL
                     FDENASCHFALGKAYASCLEGGKNMNAEEQKKAGINDSFIHVDFMVGTKDLEIDGILA
                     SGEKIPVFRDGNFVF"
     assembly_gap    10037..10136
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10394..11233
                     /locus_tag="PKW26_04860"
     CDS             10394..11233
                     /locus_tag="PKW26_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOQ92928.1"
                     /translation="MIENKIQKSEIEYKLSFIMGKRLNLHNPDVLNLTIQDLKRNYYK
                     RAKELHPDTASQNGISESLLTERFKRLSESYNFLLEYWQNRDFSSLLKEDQYKNQYRT
                     KPNQKQKAKSTKGFQQQEQRKTAHRYRQQQGKNEAPKRFYTGPMPKTEMRFAQFLYYS
                     KKIDWNTFVQALSWQWKNRPRLGEIAAKKGYLEQSDIITILRHSFPGELFGKAAIRLG
                     FLSERELFLLLGHQKLLNKPIGLFFIEQCSFPQSEIEKQLAQMKTFNTAIKHEKQKAN
                     YKR"
     gene            11292..11795
                     /locus_tag="PKW26_04865"
     CDS             11292..11795
                     /locus_tag="PKW26_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOQ92929.1"
                     /translation="MITDSQILTAVIIFGARIVDVSLSTFRHVMIIKRKRLYAFSIAF
                     FESLIWVYAVSRVITNLTDPITAIAFALGFASGTFVGITIEGFIKIGDQAVRVFSSKG
                     DSVAKVLREMGFRITVFDGHGRDGVVKMLFVQTRRRDVKKVFKKAREIDPACFMVVDD
                     ISQAYRA"
     gene            complement(11805..12347)
                     /locus_tag="PKW26_04870"
     CDS             complement(11805..12347)
                     /locus_tag="PKW26_04870"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOQ92930.1"
                     /translation="MKKLMLGIGDLVKVKNEEIELWTVLGSCVSIIFHIPDAFSLMCH
                     AQLPELSREAQERAASAPLQDSRRYIHVDTVLAKMMAIVMQEGFSPKDVDVSLIGGCS
                     AQQAHLSHFAVGEKNVEAAKKMLGQNGYIIRRERTLMENGVTLWYYPKTNVLKYRVHN
                     ALATDFVQAPEEKRKVIELR"
     gene            complement(12363..13124)
                     /locus_tag="PKW26_04875"
     CDS             complement(12363..13124)
                     /locus_tag="PKW26_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92931.1"
                     /translation="MTKREKPARAKKKPVSGFRKKFFFILRATILLSVFLNLGMGAYI
                     VLRDGNVSQPEVVEELNERLEYIVWALVTFVLTYGTSYIEKNRKINIPDVLELVITLF
                     IYAGIFLSNQFNLYYKFWWWDDLLHFFSGIIIGFIGFIVIYLINHNYSMDISPLLVAF
                     FTFTFAVTLGVFWEIGEFTLDALLGHDHQKWGLPPDSVLLGKPYQGSGLRDTMSDLIV
                     DCLGALITSFITYFAYKKDKKRTLEQMKTIINTEE"
     gene            complement(13133..14236)
                     /locus_tag="PKW26_04880"
     CDS             complement(13133..14236)
                     /locus_tag="PKW26_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOQ92932.1"
                     /translation="MSKIEEFEAIKKCLSIIVSTSPLYKNKKVFVFFIKNAKAGGLIS
                     KAKTNRYLNAFHDIAQQQKKKPVLAKAVDVSVMETQRARHAQVFTESIVDMAVSNKED
                     NEYLIVSAGGDGTSWEIQSVLMTQSLKNKKTQTVLKEKVSFLALALGTGNDGADGKTL
                     EESLSFLTAGKAFTYKKAVMIQTSKDMPEFGSKTAFSFNIASVGIDAFVNEMTNKTKS
                     FLPGNFYKLWVNISALFYETKYPLGPMEVKLYDKNKDCFQSFNEPLLFCLLGASGKRT
                     YGSGHKILPGEENFCMARQMPLHTKIQNLSAFKEGSHIGKPFTHLDFASKISIFYDRA
                     ILLQIDGEVAALDKASFPLTMELVDFHIKKITK"
     gene            complement(14302..14985)
                     /locus_tag="PKW26_04885"
     CDS             complement(14302..14985)
                     /locus_tag="PKW26_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOQ92933.1"
                     /translation="MKLLYKGKTKDVYEIDADTVLLQFKDDVTGENGVFDPGANTVGL
                     SIDGMGKSGLRLTEFFFKKLSEKNIPTHFISADIENATMTVQKAEAFGKGLEVICRYK
                     AVGSFLRRYGMYAKEGQNLDAFVEVTLKDDARNDPPITKDALAMLNLLSHEEYDILKK
                     MTQEISGIIKDELAKKGLVLYDIKLEFGRVGADKHIALVDEISGGNMRAYKDASYVEP
                     MKLEQIMLS"
BASE COUNT         4637 a         3148 c         2563 g         4514 t
ORIGIN      
        1 cgtaaaaaca tagttttcct tagtataaaa ataatagctt agcaaaaact ctaaagattt
       61 cactaagcta tctcattgta aaggaatcat cttttcttgt aaacagaaag gatacaggac
      121 atactagttt gcagcgtcaa gtttttccct taaacttttt ccccatgcaa atgctttttc
      181 acttgcatca gagtctggat tccaaagcac cttatgcccc tcatctaaga tttcaaaacc
      241 ggatcgtttt aaagcctcgg taatctttgc aggaccttca ccactccagc cataacagcc
      301 aaaaccagca gcttttttcc ctttaaatcg aagcccttcc atcatttcca agattccagc
      361 ggtagcatgc gtcagccctc gcccaatagt tggcgaaccc accaaaatca tcttactctt
      421 gaacacttca gtgagaacat cgtttttatc gtttttggct agattcatga gctttatagt
      481 acattttttt ccagtacaag gcttatctcc caaaccttcg gatatagcct cggcaacctt
      541 tcgagtacct tcccacatcg tgtcgtaaag aatcgtaatt tgattttctg cgtaatcctg
      601 tgaccattgc agatattttt ccacaatttg tgcgggattt tctcgccaaa taaccccatg
      661 acttggtgca ataatatcga ttggaagctt caatgccaaa acttcttcga tttttttatg
      721 cacgagcatg ctaaaaggcg ttagaatatt tgcatagtat tttatacact catgatacaa
      781 ggcatcctta tcaaccttat cgttaaaaag ctcatgactt gccatatgtt ggccaaaagc
      841 atcgttacta aacaagatat tatcaccggt aagataggtc gccatactat ctggccagtg
      901 aagcattttc atttcaacaa atacaagctt ttttccattt cctatgtcga gactgtctcc
      961 cgtttttact gttttgaagt tccagttttg atgatactgc cccgtcaaag atttcaccgc
     1021 agcctcagta caataaatag gtacgtctgg aatatgactc atcagctcac aaagtgctcc
     1081 cgaatggtca atttccccgt gattcataat aacatagtct attttttcaa gcggaattgc
     1141 ctcttttaca ttttctacaa attcttttgc atagggcaac cacacagtat cgataagtac
     1201 atttttttct tcctgtataa gaaaagaatt atacgaagaa ccagcatgag tcgaatactc
     1261 atctccatga aatcgctgta attcccagtc aatttttcca acccattgaa cattgttttt
     1321 tacggtaata cgcattctat tcctccaaaa aaactcttaa tgtaaagcta aaaaacctag
     1381 ctaataacta gtatatcata gctttaaaaa aatatctccc caaaaaggca ttttttccct
     1441 tgatagattg catttttcat aggataataa taggagaagg agacgctctt gaatgatcta
     1501 ggccgaaagg tattttttgt atatcctccc agtgtagtta aagaagactt aattnnnnnn
     1561 nnnnacgaat acgaaaccta catggtaaaa gatacccgtc gcgctgaact tttatttgaa
     1621 aagtacgatg atgccatttg ttttgtaaat attgacaccg ggttttctga accagaatgg
     1681 gaaacgtgga tacggaaaat acttgcagaa ccgaaattta gcaaaattgg cattggaatt
     1741 gtttcctaca acagcaatga aaaactacaa aaaaaatatc ttatggatat aggtattcaa
     1801 tgtggctata ttaagctaaa acttgggcgt gatgaaagca taaaaatctt acttgctacc
     1861 cttaatgcaa acgaagcaaa gggaagaagg aaatatgtca gagcagatgc cacaggagat
     1921 gcactcagta gccttaactt tactcatgga cgcttacaaa ttataggaaa aattgtcgac
     1981 atttctgtcg ttggctttgc ctgcatacta gacccagacc ccgaattagc aaaaaacatg
     2041 ctcttgagcg acgtgcaact aaaacttcgc gcaagcttgc ttcgaacaga ccttattctc
     2101 ttgggctcgc gccccgtaga cgacagaaac cagtatattt ttttgtttcc cccaaaaata
     2161 gaaaaccatg caaaagcaaa aattcgaacc tacattcaaa caaccttgca agcaagccta
     2221 gaacagtatc ttgaagaagc actcaaaaat gcaccgaagg aagcacctcg ctcaagtcca
     2281 caaaacacaa gccccttact cgagccagca ctcgaagagc tagaagattt agcggaagtt
     2341 gaagcaattg atgaggctgc cgaaaagccg taagcaaagg gagtcaacaa agaaggtgaa
     2401 aactaaagat taactcgtaa agttgtcaaa atagccttga atatagacta tcggggtgcc
     2461 cttgtcaccg cttccactcg ttaaatcact tagagagcca acaaggtcta caagacgtcg
     2521 aggagttgtg ccctgactgg ccatatcctg agaatctgtt tcgctttttt cttttatgct
     2581 ctttgaaatg gcttctttta aagcctcccc agaaagagat gcaaactcat tatctgcgag
     2641 atattttagt tttatttcgt ggggttgccc ttcaagccca ccagtataag caggagatac
     2701 aaccgggtca gcaagctccc aaatcccccc gatggggtcc ttgaaggcgc catcgccata
     2761 gaccatgacc tctacctttt tccctgtttt ttcaaaaatc tgctcttgaa ttgactcaac
     2821 taccttggca cagttacgcg gaaaaagctt tacgctttct tccgtagctt tattgcttcc
     2881 caaaagccca aagtcttcgt tgtacccact ttttccaaca ggttttttca agcaatcttc
     2941 aagaccgtac acttgttccc caccagcatt tttcaaaagg cgcatcgtgc gttttcgcgt
     3001 atgcacatcg caagcgagga cattttttgt gtattttaat attgcccgag gatcgttcgc
     3061 aagaacaatt tccacctcac aatcttctgc ttcaacaata gatttataat aggcaatata
     3121 atctatcccg gtaaaacggt gtccaggaaa accaaaagat ctgcgaaaat ctgcttcact
     3181 gagaacatcg ctccagggat ttatgccctt ttcgtcgagt gcatccaagt ctacaagatg
     3241 attcccaacc tcatcggcgg gaaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnccacta cgaataatgg gtgcgcgaat tcctctcgaa acagtaccga tgagccgttc
     3421 catctttcct ccaaaaccct tgtgcaggct acaaaatctg taaaacaagt acacgcaagt
     3481 atttgaatta cacactataa tagcgttttt tgcgattttg agcaagagag gaggttttag
     3541 aaaaaaaagg ctaccctttc gagtagccct tcgttttaca atttttattg tattacctct
     3601 gccgcggtat attcctccat ctttgcaagg cttgaaatat cctctagtaa gacttggctt
     3661 gaactcaaca aagcaacagc ttgatctttt acatgggtcg tcagctcatt tacttgcgta
     3721 agggcgtcaa gaacttgctg tccaccaaca ctttgttcat ctgcagcttg ctttataaca
     3781 agcccttgat ttcgcacagc ctcacttaaa ttaagcactt gcccaaactt ttttaaagct
     3841 tcctctgatg agccagagac ctcatctatt aaggttttta gttgatcgag cacccttgaa
     3901 atagatgaag cttgacttcc agcattttct gccagttttc gaatttcatc agctacaact
     3961 gcaaagcctt taccatagtt tccagcatga gctgcttcaa tcgccgcatt cattgccaaa
     4021 agatttgtct gactcgctat ttgttgtata acagcactcg cctctatcat cccttcagat
     4081 tgatttgaaa tttgttctat gagcgaaaca atcatatcta gcccttctcc gcccgacttt
     4141 gatgcttctt ctagttcaag aacagcactt gcattatctc ctaggattct atttattgaa
     4201 tcaacatttg caaccatttg ttgaattgca gcagaagact ctacgacaaa gtttgccatt
     4261 tcagtcgagc cgtcagctaa aaatgaaata tctttcatgt tttgttctaa gcgttttata
     4321 ctttcacctg aaatagattg tattacttca ttgatttcct gtttatggct atcatgaagc
     4381 attgcaactt caacggacat ataatgtcta cagttctcag ctctatttaa accattcact
     4441 attgcaacgg ccatctgctc acagctatta tatccacaag caccgcagtt taaaatatcc
     4501 tgaggctttg ttttatgcat atctttatac acgcgttcaa tttcagcatt tgaaggatta
     4561 cgaaccatat ccttaaaaac cccagaacga tctacgtaag tcctgtcata caaacctttt
     4621 ctccagtatt tgttcagaac agcattaaat ttcttttgag cacgttttgg attttttaac
     4681 aaggcttttg agcgtatttt ttctttatat tcctcacttc gccgttcaat cataccttct
     4741 acgatatcag ggtgtttccc cttatttgat gttcccggtc caccgttaca acccatctca
     4801 caatttaagc agtcaacgag ttcataaata ggcgcttctt ttttttctat tgcatctcct
     4861 aaatgagcta aataatgata gacttgtgga tgcccttcaa tcttccgcgt tttttcaaat
     4921 attcctggaa cttcccgacc tgctgttctc ataagcccac ccggtgttga aaataaaact
     4981 gctcgctctg ccatgggaga atcaaaatct ctctctggat aatcagatat ttttacatca
     5041 tggtctttta tatatttttc aaacgaagta aaggcgacgt tataatcccc aagccctata
     5101 tcatcaaact cacgtttttt tgaaaagcag ggagaaacca caagaatctt atgattttta
     5161 aacttcgggt aaaactcttt tatcatcttc ataacatgag ccatgggact gtcgcaggga
     5221 gcaagaaggg gaataagctc gggtcgatag atttcaacaa agctgacgag ggtaggacaa
     5281 ggttgtgcta taatgcaact gggatttgca tcttttttat attcaagata ggatttaaca
     5341 gttagttccg caccaaaact tacatcaaaa aaaccttcca ctcctagcga ttcaagccag
     5401 ccatttagct gcagtatctt cccgttaaaa cttgccgcgg cagctggagc aacaatggca
     5461 acaatttttt cttttttagc taaagcttca aaaaaaactt ccgcatcatc tatacctata
     5521 cgagccccat gggtacaagc ttcgatacaa gcgccacagc caagacataa gtcactgtga
     5581 agttctacat agtcccctga gccgtcattg cacatttttg cagggcaaac cgctacacaa
     5641 cgatggcagt taacacaatt ctcctctatt accttaatta caggtcgcat tggttttagc
     5701 atatactttt ctcctttata catatatctt actgtagcca ttcttatagt tagcaatcta
     5761 taaagaagta tcggcattat atttattttc tccacttact gtataaaata ctgcttttat
     5821 gttatactat tagtgagtta tagtttataa acgaggtttt ttatgtggat tttctttgct
     5881 ttctcttctg ctttttttgc tggtttggta gctattcttg ccaaaattgg cattaaaaat
     5941 acagattcaa atgtggcgac tgcaatacgc actgtcgtca tagttctttt ttcatggatg
     6001 atggtgttta ttgttggctc gcatacaaaa attgccgaga tttcagcaaa aacccttgta
     6061 tttcttattt tatcaggctt tgcaaccggt ggctcatggc ttttttactt ccgtgccttg
     6121 caaataggcg atgtaaacaa ggttgtccct gttgacaagt caagcactat tttaaccatt
     6181 attctttccc tcatcatttt ccgcgaaggc gtcagcatcc caaaaatcat tgcggtaatt
     6241 gcaattgcaa ttggcaccta tctaatgatt gaaaaaaaac aaacaaaaga atcgggagaa
     6301 ggtggttata aatggctcgt ctacgcaatt cttggggcgg tttttgcgag cttaactgct
     6361 atcctgggga aaattggcat acaagacatc gactcaaacc ttggaacagc gattcgaacc
     6421 atcgttgttc tcattatggc atggcttatt atcatcgggc agggaaaagt gggacagcta
     6481 aaacacatcg ataaaaaaag ctggattttt ataatccttt cgggatttgc aactggtctt
     6541 tcttggctca gctattatcg cgccttacaa acaggacctg caagcgtcgt tgtacccatc
     6601 gataagctca gtattgtctt gagcgtcgcc tttagttatt ttatcttaaa agaaaaatta
     6661 aagaaaaaat cttttttagg cttagcttta atcgtagcgg gtactcttac actcattttt
     6721 tttaagtaat aaaaaaacta aacaaaaaat agaaaatacc gacactttaa aagcatagtt
     6781 tctggagaga aatagcacat tgcactggag atatatatga aaattgcaca aaaactttta
     6841 cttattatca caggagcttt aattaccttt ttggtagcca atgttatcat tgtaggctct
     6901 cagtttacac aactttcaga agatataaca atagaagacg tcgcttctaa gctccgtagc
     6961 aatataaatg ccgcccatct ttttttagaa gatacatacg aaggtattat tttgaaaaat
     7021 ggatctatgg ttgggaccaa tggaaaaaat gtagaaaata atactgagtt tgtcgacttc
     7081 ctcaaaagca ctttttcgac acaggctact atctttaaac gcgaaggagg agatttcaca
     7141 cgagttgcaa catctattct tcaggatgac ggacagaggg ctgttggaac aacattaaat
     7201 gaaaacaata taatatacca aaaactaact gatggcgaaa catacgtcgg tgaaacaaga
     7261 atattagaca atccatacat agcaatctat agtcctatct taggacaatt tggtgaagtt
     7321 atcggacttt tatttctcgg tgaaaataaa gagacgatac agtcaatgat acgatcttac
     7381 caggtaaagg gttttctcat tgtagtaagc tcatctatac tagctattct aataattgtt
     7441 gccttttcat atttacttat aaagaaaacg attttacacc caataacaaa cacggttagt
     7501 ttattaaaaa acatttctga aggagatggt gatttaagcc aaaggataga aatccagtcg
     7561 aaggacgaaa ttggcgactt agcaaaatat tttaatctca cggttgaaaa aatagggcaa
     7621 actatcttat tggtaaaaaa ccaaacaacg caactaaagc tttcaggtga gcttcttgca
     7681 actaatacaa atcaaactgc aacggcaatc aatcaaatca ccgcaaatat cagtagcata
     7741 caaaatcaaa cagtaaatca atcagcaagc gttaccgaaa cgagtgctac gatagagcaa
     7801 gtttcaaaag gaattgaacg gctaaacgaa cttataacag atcaatctgc aaatattaca
     7861 caatcttctt ctgcaataga agaaatgatg gcaaatatac attctgttac tatgtcactt
     7921 gtaaaaaatg aggcaaatat tagccgactt acagagtctt cgcaaagtgg taaaacgcaa
     7981 gtagagaaaa tcgcaaatgc aattacagat gttgcaaagg actcccaaag cctccttgaa
     8041 gtaagctctt taattcaagg tattgcctcg caaacaaatc ttctcgcaat gaatgcagcg
     8101 attgaagcag cccatgcagg cgagtttgga aaaggttttg ctgttgttgc cgatgaagtg
     8161 agaaagcttg cggaaacatc aagcacgcaa acaaaaacaa taacagctat gcttaaaaaa
     8221 attactgcat ctattcagga aattaccgca tactctgata atgttgtttc aacatttatg
     8281 cttatggaag acgaggttaa aacagtcgga acacatgagg ctacaattct taacgccatg
     8341 aaagaacaaa atgaaggtgg tcggcaaatt ctagaagcaa tatcttctct taatacaata
     8401 acgcaaaatg tgcaccaaaa ctccgaggag atgcttacag gtagccaaca agtcctagaa
     8461 gaaacgaaga acatgaatca tattacccag gaaattaccg acggaatgaa tgaaatggca
     8521 agtggctcta aagaaataag cgttgctgtc gaacaagtaa atcaactcag tgtcgaaaac
     8581 actctaagca tagaaaatct tgcaaaagaa gtgcagaaat ttaaagtata agactctttt
     8641 tagcgactaa aaaacaaagt tcccgtctct aaagacaggg attttttccc cagaagctag
     8701 aatgccatct atttctagat cttttgtccc gaccataaaa tcaacatgga taaaactatc
     8761 gtttatcccg gctttttttt gctcttcggc gttcatattt ttcccacctt ctaggcaaga
     8821 ggcgtatgcc tttccgaggg caaagtggca agatgcattt tcatcaaaaa gagtattgta
     8881 aaacaatatg cccgtgttag aaataggcga gtcgtaggaa acaagggcaa cttcaccgag
     8941 gcgttttgct ccttcatctg tttcaaggag ctgctttaag ttttccgcac ccttttctgc
     9001 tccgaaatcg acaacgaagc catcctctat ttcaaaccaa aacttgtcta taaggtttcc
     9061 ttgataattc aatggaatag atgaatagat acgcccatct gtaccctttg catctggcgc
     9121 tgtaaaaact tcttctgttg gcatatttgc attaaaggga tagcctgtct cagaatcttt
     9181 actaccaccc aaccagaggt gaccttttgc gagagcaaca cgcaagtcgg ttccaaggct
     9241 atttttatat tctagatggg taaaggcata gtcatttaaa atctttgtac gcctttcaaa
     9301 gttttcttga tgtttttccc attccaaaaa ggggtcttcg cagtcaactc gagttgcctt
     9361 gtagattgca tcccaaagtt ttttgactgc tacttcaggc tcatccttgg ggaaaacttt
     9421 tcgagcccag ccctcacttg aagcagctgc aacacaccag gcatttcttc cgctcatttg
     9481 ttcatttcga aaatgttcaa gcgctataga acgggccttt accgctcgag ctatacgctt
     9541 ggggtcgaca tccttcatca actcgggatc atctgaaaca atggtcataa aagcagcttt
     9601 ctgatctaca tagccgttat aaaacgaaga aacccaaggc gctacctcat caaagatagc
     9661 atcgtcggcc atatcatagc taatcttcga cgacttttca tctatccagc gcattacaac
     9721 ttcgcgagcc cctgccttgt atgcttcttc ttgaagcagg cgtccaaaat cggcaacttc
     9781 aatagggcac gacaaaacaa aggtttgtcc tttttgaata ttgactccct gccgaacaag
     9841 caacgctgca tacttttgtt ttaagttcat atctacccca ctaaaaaaat tatatctaca
     9901 aaaattattt atagaaccat tctaatagca aggtaaaatt tatgcaagat aaaaaaaagg
     9961 ctgcccgctt tggacaacct tttttttgtt ttattataaa tacttagcgt gctgcaagca
    10021 atgcttttac tgcaagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgat
    10141 acttccaatt tgtatcttga aaatcatcat tttgcgcaaa ataaaaacca tcttcaacgt
    10201 tatctaattt tgcagttgat tttgtgcatg aaatagagaa aacgagtacg aaaactgaaa
    10261 gaacaacaaa taatgcaata aatatttttt tattcatact aagctcctta tgataaaaaa
    10321 tagcaaacaa aaggcgtatt gtcaataagc acagcgtagc aaaaaaagta cttttgctgt
    10381 agtatatcat actgtgatag aaaataaaat acaaaaatct gaaatagaat ataaattaag
    10441 ctttataatg ggtaaaaggc ttaacctaca caatcctgat gtactaaacc taactattca
    10501 ggacttaaaa agaaactatt ataaaagagc aaaagaatta catcccgaca ctgcctccca
    10561 aaacggaatt tcagaaagct tactcacgga acgctttaaa cggctttcag agtcatataa
    10621 ttttctatta gaatattggc aaaacagaga tttttcgagc cttttaaaag aagaccaata
    10681 taagaatcaa taccgaacaa aaccgaatca aaaacaaaaa gcaaaatcca caaagggctt
    10741 tcagcagcag gaacaaagaa aaacagccca tcgatatcgg caacagcagg gaaaaaacga
    10801 agcgccaaaa cgattttaca caggtcccat gccaaaaacc gagatgcgct ttgcacaatt
    10861 tttgtattat tctaaaaaaa ttgactggaa tacctttgtt caagcactca gttggcaatg
    10921 gaaaaataga ccgcgcttgg gcgaaatagc cgcaaaaaag ggatatttag aacaaagcga
    10981 tattataact atcttgcgcc actcttttcc cggtgaactc tttggaaaag cagctattcg
    11041 tctcggtttt ttatctgaaa gagaactgtt tttattgctc gggcatcaaa aacttttaaa
    11101 caaaccgatt ggactttttt ttatcgaaca atgcagcttt cctcaatctg aaatagaaaa
    11161 acaacttgca caaatgaaaa cctttaacac agcaataaaa cacgaaaaac aaaaagcaaa
    11221 ctacaaaaga taagtatgaa aagttttttt ccatacttgt attaaatgct tttttttcgt
    11281 atcttacata aatgataaca gactcccaaa tacttaccgc agtcataatc tttggcgcac
    11341 ggattgttga cgtttcactc agtacctttc gccatgttat gattataaaa cgcaaacggc
    11401 tctatgcctt ttctatcgcc ttttttgaat cacttatttg ggtctatgct gtttctcgcg
    11461 ttataaccaa tctaactgac cccattaccg cgattgcctt tgcacttggc tttgcatcag
    11521 gcacctttgt aggcattacg attgaaggct ttataaaaat aggcgaccaa gcagtacgtg
    11581 tttttagttc aaaaggagac tctgtcgcaa aagtcttacg cgagatgggt tttcgcatca
    11641 ctgtttttga cgggcacgga cgagatggcg ttgtaaaaat gctttttgta caaacaagaa
    11701 gacgtgatgt aaaaaaagtt tttaaaaaag cgagagaaat agaccccgcc tgctttatgg
    11761 ttgtagatga tatctcacaa gcctacaggg cctaaacaca gaaactatcg aagctctata
    11821 acctttcttt tttcttctgg ggcctgcaca aaatctgttg ccaaggcatt gtgtacacga
    11881 tactttaaga catttgtctt cggataatac cagagcgtaa caccgttttc cataagcgtt
    11941 ctttcccgcc gaataatata gccattttga ccaagcattt tttttgcagc ttcgacgttt
    12001 ttttctccaa cggcaaaatg cgatagatgc gcttgttgtg cgctgcaccc ccctatcaag
    12061 ctgacatcaa catcctttgg agaaaaaccc tcttgcatca caattgccat catttttgca
    12121 agaaccgtat cgacgtgaat ataacgtcga gagtcttgca aaggtgcgct tgcagccctt
    12181 tcttgagctt cacggcttaa ttctggtaac tgagcatgac acataaggga aaaggcatct
    12241 ggtatgtgaa aaattatcga cacacaggaa ccgaggacag tccagagctc tatctcttca
    12301 ttttttacct taaccaagtc tccgattccg agcattaatt ttttcacaaa gtctccagaa
    12361 ctctattctt ctgtattgat gatagtcttc atttgttcaa gagttcgttt tttatctttt
    12421 ttatatgcga aatacgtaat aaaagaagta attaaagcac ccaggcagtc tacgatgagg
    12481 tcgctcattg tatcccgcaa accagagcct tggtagggtt ttcctagtaa aacagagtct
    12541 ggcggaagcc cccatttttg gtgatcatga ccaagaagcg catcgagggt aaactcacct
    12601 atttcccaga aaacgccaag tgttaccgca aaggtaaagg taaaaaaagc aacaagaagc
    12661 ggacttatat ccatgctata attatgattg ataagatata taacaataaa cccgataaaa
    12721 ccaatgatta tgcctgagaa aaaatgtaag aggtcatccc accaccaaaa tttatagtaa
    12781 aggttaaact gattgctcaa aaaaataccc gcataaataa agagcgttat aacaagttct
    12841 aaaacatcgg gaatatttat ttttctgttt ttttctatat agctcgttcc ataggttaag
    12901 acaaaggtaa caagtgccca tacaatgtat tcgagacgtt cattcagttc ttcgacaacc
    12961 tctggttgac tgacattacc atctcttaaa acaatatatg cacccattcc aagattaagg
    13021 aaaacagata acaatatggt tgctcgtaag ataaaaaaga atttttttct aaatcctgaa
    13081 acaggtttct tttttgctct cgcgggcttt tctctctttg tcatttttca cctcattttg
    13141 taattttttt tatatgaaaa tcaaccaact ccattgttaa gggaaaagat gctttatcta
    13201 aggcagcaac ctctccgtcg atttgcaata aaattgctct atcatagaat atacttattt
    13261 ttgaagcaaa atccaaatga gtaaagggtt ttcctatatg actgccctct ttgaatgcac
    13321 ttaaattttg aatttttgta tgaagtggca tttgtcgtgc catacaaaaa ttttcctctc
    13381 caggcaaaat cttatgtccg ctgccatagg tcctttttcc tgaagcaccc aaaagacaaa
    13441 ataaaagtgg ctcattaaaa ctttgaaagc agtccttgtt tttatcatag agttttactt
    13501 ccataggccc caaggggtat tttgtctcat aaaaaagagc gctgatattt acccagagtt
    13561 tatagaaatt tcctggtaaa aaagattttg ttttattggt catttcattt acaaaggcgt
    13621 ctattccgac acttgctata ttaaaagaaa aggctgtttt actaccaaac tctggcatgt
    13681 ctttactggt ctgtatcata acagcttttt tataggtaaa ggctttacca gctgttaaaa
    13741 aagaaaggct ttcttcgaga gtttttccgt cagctccgtc gtttcccgtc ccaagagcaa
    13801 gggctaaaaa tgaaactttt tccttaagca ctgtctgtgt ttttttattt ttaagggatt
    13861 gcgtcataag aaccgactgg atttcccaac ttgttccatc gcctccagca gaaacgatga
    13921 ggtattcgtt atcttctttg tttgaaacag ccatgtcaac tatagattct gtaaacacct
    13981 gagcatgtcg agctctttgc gtttccataa cactcacatc tacggccttc gctaggacag
    14041 gttttttttt ctgttgctgt gcgatatcat gaaaagcatt taagtagcga ttggtttttg
    14101 ctttgcttat aagaccgcca gcctttgcat tttttataaa aaacacaaat acttttttat
    14161 ttttatacaa gggactggta gaaacgatga tactaaggca tttttttata gcttcaaatt
    14221 cttctatttt tgacatactc tcattatgta agtcaataaa agacttgtca agctttttat
    14281 tgttttctta atgtatggcc tttaggaaag cataatttgt tcaagtttca ttggctcgac
    14341 gtaagaagca tctttgtagg ccctcatgtt ccctccagag atttcatcta caagggcgat
    14401 atgtttatct gcaccgactc gaccaaattc aagctttatg tcatagagca ctaagccttt
    14461 ttttgcaagc tcgtctttaa ttatccctga aatttcttgt gtcatttttt ttaagatatc
    14521 atattcttca tggctgagga gattgagcat agccaaggca tcttttgtaa tagggggatc
    14581 atttctcgca tcatctttta aggtaacttc aacaaaggca tcgaggttct gcccttcttt
    14641 tgcgtacatt ccgtaacgtc tcaaaaaact tccaacagcc ttatagcgac aaataacctc
    14701 aagccctttc ccaaaagctt cggctttttg cacagtcata gttgcatttt ctatatcagc
    14761 agatataaaa tgtgtcggga tatttttttc actgagtttt ttgaaaaaga attctgtgag
    14821 gcgcaagcca gattttccca taccatcgat gcttagccca acagtatttg caccgggatc
    14881 aaaaacgcca ttttccccag ttacatcatc tttgaattgc aacaacacag tatctgcatc
    14941 tatttcatag acatcttttg ttttaccctt atacaataat ttcatctttc ctcccaagat
    15001 tacgagtctc ttttataaaa aaacagtatc attttatgac tttcctatca ataagaaaat
    15061 gaatgttttt tt
//
LOCUS       DAOSSW010000041        14933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_4093_length_14933_cov_7.683627, whole genome shotgun sequence.
ACCESSION   DAOSSW010000041 DAOSSW010000000
VERSION     DAOSSW010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 14933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14933
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4093_length_14933_cov_7.683627"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PKW26_04890"
     CDS             complement(<1..1064)
                     /locus_tag="PKW26_04890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HOQ92934.1"
                     /translation="MQKKNNSNIQASCKKQSTQAATRMTIKEARQNAYRQLSEAWEKQ
                     KKTAGPYAQKPDFFLDIDVILQELLQVERSFLLAHNETKLKQKDIKRFFALIQKRSRG
                     LPIAYIVGKKEFYGLSFFVSPHVLIPKPDTEILVEKALELAREKSRKIGSQEVQAESR
                     ETGSQEVQAKSHETASREVQAKSRETGNREVQAESRCLRVLDLCTGSGCIALSFAQEF
                     CKTKENSPPLELFMSDISLAALRLAKKNTKQLFPASRKKQKNSDKLQIKIVRSNLFRN
                     IKGNFDFILTNPPYVPTRLTDELLLDGRSEPRLALDGGKDGLRLIRQIAKDAKNYLKK
                     DGWILIEAGEYHAKEVAQIL"
     gene            complement(1024..2097)
                     /gene="prfA"
                     /locus_tag="PKW26_04895"
     CDS             complement(1024..2097)
                     /gene="prfA"
                     /locus_tag="PKW26_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOQ92935.1"
                     /translation="MRKKLDELAKRHEEVNILIQDANLIKDQKKYREIMREHAHLEDL
                     MEAYEKYKTVLSGIEEAKAIITQEDDHDMKEMAREELKSLEEKVPEMEDSLKMLLIPP
                     DPLEEKNIIMEIRGGAGGDESSLFAADLFRMYTRYCEMKGWKYEIMNANETEVGGYKE
                     IAFSISGKYVYGSMRYEGGVHRVQRVPETEAQGRIHTSAVTVAVLPEAEETEIEIKNE
                     DLRVDVMRAGGPGGQSVNTTDSAVRLTHIPTGLVVIQQDEKSQIKNKAKAMRVLRARL
                     FELEESKKNAERSAARKSMVGSGERSERIRTYNFPQNRLTDHRINLTLYKLDYIMQGY
                     LDEMIDALCIYAKEEQLKHSSFL"
     gene            complement(2164..4884)
                     /locus_tag="PKW26_04900"
     CDS             complement(2164..4884)
                     /locus_tag="PKW26_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92936.1"
                     /translation="MFSFLKRVFKSKNKVAVQDFEQSAFENWASSFSNPETRRFIEEE
                     GDGYKTQFNKGLELQLLRKNLYAWTVNPQFQYKNLILQGTIDFSKTNLSKRLANKLSQ
                     DAQAGSAAFGFLFRYVDEANHLSFLISNQGFFRLDAYFNGNQFPLIGWTKCPPPPRSL
                     NKNKESETKETKKSLGKIPLTILAYETKIAAIINGRLALMLDDDTVQSEGYIAFAGQN
                     WTSEEVSQASLLNISLESRQMQVSAFASDLIENAYIPSDARIQLALSYAAVSRMVPAL
                     IEVKKALNQGISKRADYVFAARLLMSQKLYDEAENLLHKAEEHFPNKIEIIEEKAALL
                     YLWGRYNELDNFLALHSEITKDSSRIASLKAHLFAMRFSYTEALNHYLHASRLAPEER
                     EHLENASQMLDALQKPAEAQEMRFSILKDSFEKNDFQSFYSLLELIKSKDAKLLSKKH
                     TTELLALEGLSLFYIATANTEHIDSERKTNKDTAKKTLQKYYKSIQKEKTCCNKEALV
                     RLASILKEEGETKKAIEVLEFCIENANEKKQSKQSEDSSIPTEHNCSLEAYRELSELY
                     AGIGEVEKSLETINEALRIKADDGWSLYIKAHIFFSTERWDEAYETIFACLAVLEEEL
                     AVLDLYCKIMEKKNKLPQALGSLEAIAKQSGAGSDFRSDALHLAANYYLVLSNNKKAE
                     QLYKKALALKPRDESLLCDYASLCIDLERINEADALMSNILAETKTPRVFQILASISA
                     KKGDFVRSEIILKQALEEPISDYWRVSLLIDLIHLYLFTNKTKQVESLLDEVVSIEES
                     ERTLALQNEIREKNQRKIICALCEKTWFVPKTIPGNARLRITCQPPDDYPAGSCLECE
                     KTYCISCVKESLSDEGRFHCPTCKKPLKIQDAGIFWILNEWQKSYDSKNQ"
     gene            complement(4921..5640)
                     /locus_tag="PKW26_04905"
     CDS             complement(4921..5640)
                     /locus_tag="PKW26_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HOQ92937.1"
                     /translation="MKKIFFISFIFIYLLFSAFSGSLYTEQELYDRQNLISSIRRVGE
                     PFIQDGYIVFTAKEEYRFVGISFDFEQYKKIHAFERLPFFDMDYEPLDSVLFYLCKIP
                     QNTKRIQYRLIIDGLWTNDPLNSAIRIDETTQMKLSYVDLPYEAPVITKKTEDGFVRF
                     IYEGEPGQIIGLSGSFTGWDPYIYQMEETKQGFYELRLHLPPGTHYYTYYKGFTSFVD
                     EKNPERAYTEEGRVASVLRLN"
     gene            5740..8868
                     /locus_tag="PKW26_04910"
     CDS             5740..8868
                     /locus_tag="PKW26_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ92938.1"
                     /translation="MPGLEQLQKFSQDITGLGDEPAIREERGEPFSVLPIPPDVSENN
                     DSDDFLLGMPQEGEDNSLLDLDETEELGGIDDGEQDLADFASSFDLPDLDPSADFEDV
                     FLSKDTTEQTEALDETVKSLDEDIEVLGKDLENADALSDLAAFVPDVTIDEDLESFEE
                     NVDDLDETFEDLDTLPDIDALSEIESDTEELIDVENEEDKSVLDILGEFEKENLAKET
                     PEVEVLDEFEDLSETSDNLSDFDMLSTTEEPTKAETADGSDDLESLAMDLSGDFLEPD
                     TPGQLVPDYSDFDVTTDTAQDMDAPIEDFGDFDIDNLPPLDSASLSSLNDFETTDGIL
                     SQEEEVLDDDFHIPGFSDFQLDGEGREVEKKTSGIIPGISEAESRTSLSEAEYKTFKE
                     NLQHYPLNLKIAIQEVIVKNEFTDDAIFGLIEKVLQKSTARQVSSFMSKYLDISVDVP
                     RDFEKRSVAQYAAYKQSLEYQLKNRLVPALIIGTAAALLLGAASFLIHNFVYKPIKAE
                     GLYKEGYALLEENLYVQSEELFNEAVYYNPVKKWFFTFAEGYREKKQYERSRTMYNRI
                     LARYKNDKKAGMSLAEMELYDLANYSQAEEVIKRKVLDYHVNDKDAMLLLGDVYLEWA
                     TEKDPALFPQAYDQYMDLIDLWGRKDEYLKRLLRYYIRIDNLREVLPLKAYFLPKKKQ
                     LESQDLIELSGYLFDKLYGDLPPRDEFLRSEIEDVRLLLERALEADPSVPEAHYNFAR
                     YFLESKNFSNAKSFLKSALDLFEMADERKAKRILKQIHSYSLLGELYVEEQEFLLAEE
                     SYAKGIRLFEKEKEMSFLEPNKDVGKIYANLADIDYFISGDLAYAKQNYENAIANTYD
                     NASVRYKIGFIQYTNKKYAEALGSYIKASDEKPEDYHLLLALGNSLFYRGDIFASQAY
                     YERLLDLVDLQRTRLGIMFPQVRTDHADLVSMYLNASNNLGVVLSKRAKSTGDSNLNA
                     DAMLYLTESLRAWDALTRNQETMIRLEGSNLAQQNIKYLTNPLSDYDVSIYSGIPRTL
                     YGEKNIEQSYLK"
     gene            8903..9502
                     /locus_tag="PKW26_04915"
     CDS             8903..9502
                     /locus_tag="PKW26_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOQ92939.1"
                     /translation="MDGALVQQKKTELIKELFRKAIHMLTVFIPFLLRIAYYPVILLL
                     TLVLIVYVLSEILRLHKKEFPIISHITSAAARQRDGHGFVLGPVMLAFGVILTALFFD
                     PLPAQIGITALALGDGLASLVGKLCGKICIPFTKGKTLMGSLSCFIAIFLSTLCITRS
                     LAPSLIIAFAGTLIELIPIKDVDNILIPLSLALLAHFII"
     gene            complement(9513..10340)
                     /locus_tag="PKW26_04920"
     CDS             complement(9513..10340)
                     /locus_tag="PKW26_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92940.1"
                     /translation="MTLRSRNRLLHIFSYTSALLFVLSLALFVALFFRNQEPAHFFEI
                     SIAKNHFSLPFFQSSFFASILSLFILLLYLPLTGFFLLRNFEKTQAAELIYFTLFLTG
                     IFLQSLRIFVPLFNLYEGYRSILILLSRTFFSGQLLSVLSLWFIPFYSLEDPLQQADK
                     NALIIIVVAVILSFFMPINTQILEASFIYKIGFPQLFSVLKYSLISITAIALVIRGKE
                     HYETKIFLFAFSYLLLIIGSIILGSADSYALCILGSLLLCIGNSFFLKTLHAYYLWK"
     gene            10430..11161
                     /locus_tag="PKW26_04925"
     CDS             10430..11161
                     /locus_tag="PKW26_04925"
                     /inference="COORDINATES: protein motif:HMM:NF021529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2259 domain-containing protein"
                     /protein_id="HOQ92941.1"
                     /translation="MNTIQDKIAKKFFLASICILCTAFFLFAGDVAEFKDLGFSEDGT
                     KYSFAQYGIEDKSFQAWAEIYVVDIPSNNFVKNGVFSTKASVQTASKTSMTVFQELYD
                     KNKAFIERYAKKRASIDKTLYIRANESKDAGEIIRFKDYEKITSQDDVTYSLQRIIHK
                     TGSGEKTESSFYINLEKIDANGVVIATQLIGTPQLKRKGVLDYTIERVFVDDSGKNLI
                     IIVEKKIYDPRGSSIRYMVEAAELK"
     gene            complement(11154..11588)
                     /locus_tag="PKW26_04930"
     CDS             complement(11154..11588)
                     /locus_tag="PKW26_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92942.1"
                     /translation="MAFLVRKLHLLEDLYYFCDADESISEDLEKNSTIDRSEKLYVYK
                     TTVDKKNLEPKPEDFLREKKFLGYRTFDTGDFSLAKASYFFVQAQEPNETAFQKAAEA
                     IYLEALWQNIRFVDDLFYLRILEEGGKMLFQILRTIETKGTI"
     gene            11749..12750
                     /locus_tag="PKW26_04935"
     CDS             11749..12750
                     /locus_tag="PKW26_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92943.1"
                     /translation="MNKWISFMVFFALLILSTLILFVPSCKLSPQGIVFLHGDFASPV
                     LLETRIQSTQTIDFVFSEALSKFSAELKEKKTGEVLDSRFIMREEDGRFMVSLQLEEG
                     TKAGEGYELHARIFDVAGNSCDILFEFSGFNDRIPQVIINEIQTEYSKPKAEFIELYC
                     QTSGNIGGMYLYNAGDGEALAYTFPAVEVSAGEYIVLHYRSLPEEDIVDELFALDESG
                     GTYSHPEARDFWVAENKARLPKSDVILLKSSFMGEIIDAVMYSESGKTEWKTDALKAA
                     ARAVVEAGFYTSDSPVDLPCSDKVTVTRTLSRQSDGTWFVTKTSGATPGYENCTVPY"
     gene            12807..>14933
                     /locus_tag="PKW26_04940"
     CDS             12807..>14933
                     /locus_tag="PKW26_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOQ92944.1"
                     /translation="MAELLAPAGNIEALNAAIGEGADAVYMGLKSFNARLRTTNFAWN
                     QFEAAVEAVHKRGKKIFVTVNTLVEEGELERLYRFLEYLQRVGPDGLIVQDMGTIRMI
                     QEFFPDLRIHASTQLNASSAHAANLLAKEGVKRVVLARELGFSEIQAIRQKTNIELEV
                     FVHGALCVSESGLCMFSSFLGGKSANRGMCTQACRRFYGAEKPSGQEQGYYFSPYDLQ
                     LIDYIPDLVQAGVESFKIEGRMKSAEYVGTVTAAYRYVLDHWQEDKKAAIETAKRILA
                     TDFARKKTSYWYESSSAENLLDPKQAGGTGIYLGKIEKIRYHEKTKQPYIFLRGGSYE
                     PEIGDSIRLHKKDDSNRVSHKVKEILEYAGDLFIDAPKGFSNGDTVYLLQTKSMSKRH
                     PFVLPKDLSKYRRQPGAGTLPLMELERLPKNSLDYFPQGLYVQVSTVEDFHAVLHQNN
                     VRYILEFNFKTKEALIDNMESLPVSKKQVIISLDPFYPQDLEESLQQDMDDLVGLGYE
                     SFMVNNLGHFALFDRKKTNLIAGPYLYTMNRWAASFLENQNIEAFVSSYENSRKNLEA
                     SFEQVHRKKILLPLFAYPALFRMRFTLPESYDFNYFTDKEGEMFKSLSTPDGSFVLPE
                     KPFSILDKYSQMQSAGFSRFLIDLSKTKVAKGTYKQLLRSMYTSEPLEDISRFNWAEG
                     FYDPEKIAQYKKANLNRETFSKSRPRR"
BASE COUNT         4387 a         2637 c         3205 g         4704 t
ORIGIN      
        1 ctcaaaatct gggctacttc ttttgcatgg tattctcctg cttctatgag aatccagccg
       61 tcttttttaa ggtagttttt tgcatctttt gcaatctggc gaataagtct cagtccgtct
      121 tttcctccgt cgagggcaag gcgtggttca cttcgtccgt cgaggaggag ttcatcggta
      181 agtctggtgg ggacgtaggg tgggtttgtg agaataaaat cgaagtttcc ttttatattt
      241 cgaaagaggt ttgagcgaac gattttgatt tggagcttgt ctgagttttt ttgttttttt
      301 cgggacgcgg gaaaaagttg ttttgtgttt ttttttgcaa gtctaagagc tgcaaggctt
      361 atatcgctca taaaaagttc gaggggaggg ctgttttctt ttgttttgca aaattcttgg
      421 gcaaaagata gggcgatgca gccgcttccg gtgcaaaggt cgaggactcg caggcagcgg
      481 gattccgctt gtacttctcg attgccagtt tcgcgggatt ttgcttgtac ttcacgactg
      541 gcagtttcgt gggatttcgc ttgtacttct tgactgccag tttcgcggga ttccgcttgt
      601 acttcttgac tgccaatttt gcgggatttt tcccgagcga gttctagggc tttttcaact
      661 aagatttcgg tgtcaggttt ggggatgagg acatgggggc ttacaaaaaa gctgagtccg
      721 taaaattctt ttttgccgac aatataggcg atgggaagtc cacgactccg tttttgaatg
      781 agggcaaaaa agcgctttat gtctttttgt tttaactttg tttcattgtg ggcgagtaaa
      841 aaacttcgct caacttgtaa aagttcttgg agtataacat cgatgtccaa aaaaaaatca
      901 ggtttttgag cgtagggtcc agcagttttt ttttgctttt cccaagcttc gctcaattgt
      961 cgatatgcgt tttgacgtgc ttcttttata gtcattcgag tagccgcctg tgtcgattgt
     1021 tttttacagg aagcttgaat gtttgagttg ttcttctttt gcataaatac aaagggcatc
     1081 gatcatttcg tcgaggtagc cttgcatgat gtagtcgagt ttgtacaatg tcaagttgat
     1141 gcggtgatcg gtcaatcggt tttgtggaaa gttataggtt cgaattcttt cacttcgctc
     1201 gccacttccg accatgcttt ttcgagctgc agagcgctct gcattttttt tgctttcctc
     1261 aagctcaaag agccgcgctc gtaaaacgcg cattgccttt gccttgtttt ttatttggct
     1321 tttttcatct tgctgaataa caactaatcc tgttgggata tgggtgaggc gaacagctga
     1381 gtctgtggtg ttgacgctct gtcccccagg tccgccggct cgcataacgt cgacgcgtag
     1441 gtcttcgttt tttatttcta tttcagtttc ttcagcttct gggagtacgg caacggtaac
     1501 agcgcttgtg tgaatgcgcc cctgtgcttc ggtttcggga acacgttgca cacgatgcac
     1561 tccgccctcg tatcgcatac tgccgtagac gtattttcca gagatagaaa atgctatttc
     1621 tttatagcca ccaacttcag tttcgttggc gttcatgatt tcgtatttcc aacccttcat
     1681 ttcgcagtat cgtgtataca ttctaaagag gtcggcggca aaaagactgg actcgtctcc
     1741 tcctgcaccg ccacggattt ccatgataat atttttttct tcgagcgggt cgggtggaat
     1801 taaaagcatt tttaaggaat cttccatctc tggaactttt tcttctaagc ttttcaattc
     1861 ctctcttgcc atttctttca tgtcatggtc atcttcttgg gtaataatcg ccttggcttc
     1921 ttctatgccg ctgagaactg ttttgtattt ttcataggct tccatgaggt cttcgaggtg
     1981 agcatgctcc ctcataattt ctctatattt tttttggtct tttataagat tagcatcttg
     2041 tatgagaata tttacttctt cgtggcgttt tgccaattca tctaattttt ttcgcatatt
     2101 ttatactagc aaaatgtcaa aaaaataaca atgctctttt tagcatagtt ttttgctatt
     2161 gatttattga tttttactat cgtatgattt ttgccattcg ttcaagatcc aaaaaatacc
     2221 agcatcttga attttaagtg gctttttaca tgtcggacag tgaaagcgtc cttcatcact
     2281 gaggctttct tttacacaag aaatacaata ggttttttcg cattcaagac agcttcctgc
     2341 aggataatca tcagggggct ggcaggtgat acgcaagcga gcatttccag gaattgtttt
     2401 ggggacaaac catgtttttt cgcaaagcgc gcaaattatt tttcgttgat ttttttcccg
     2461 tatttcattt tgcagagcga gagttcgctc actttcttct attgatacca cttcatcgag
     2521 gaggctttct acttgcttgg ttttatttgt aaaaagatag agatgaataa gatcgattaa
     2581 aaggcttact cgccagtagt cagaaattgg ttcctcgagg gcttgtttta atattatttc
     2641 tgagcgtaca aagtcgcctt tttttgcgct gatacttgca agaatttgaa aaactcgagg
     2701 tgtttttgtt tcggcaagaa tgttggacat aagggcatcg gcttcattga tacgttctaa
     2761 atcgatacaa agggaggcat aatcgcagag gaggctttcg tctcggggtt tgagtgcgag
     2821 tgcttttttg taaagctgtt cggctttttt gttgttcgat aaaacaagat aataatttgc
     2881 agcgaggtga agggcatcgc ttctaaaatc tgaaccagct cctgattgtt ttgctattgc
     2941 ttccaagctt ccgagtgctt gtgggagttt gttttttttc tccattatct tgcagtagag
     3001 gtcaaggaca gcaagttctt cttcaaggac agcgaggcag gcaaagattg tttcgtatgc
     3061 ttcgtcccat cgttctgtgg aaaagaagat atgtgctttt atgtacaggg accagccgtc
     3121 atcagctttg atgcgcaagg cctcgtttat ggtttcaagg cttttttcta cctcaccgat
     3181 gcctgcatag agttctgaaa gttctcggta tgcttcaagg ctacaattat gttctgtagg
     3241 aattgaactg tcttcacttt gttttgattg ttttttttca tttgcgtttt ctatacaaaa
     3301 ctctagaact tctattgctt tttttgtttc tccctcttct ttcaagatgc ttgcgagcct
     3361 aacaagggct tctttgttac agcaggtttt ttctttttgt atgctcttgt agtatttttg
     3421 caatgttttt tttgcggtat ctttattcgt ttttctttct gaatcgatat gttctgtgtt
     3481 tgccgttgca atataaaaga gagagagacc ttcaagggca aggagctcgg tggtatgctt
     3541 ttttgaaaga agctttgcat cttttgactt aataagctca agtagcgaat aaaaactttg
     3601 aaaatcattt ttttcaaaag aatcttttag aatcgaaaat ctcatttcct gagcttcggc
     3661 aggtttttgc aaagcatcga gcatttgact tgcgttttca aggtgttctc tttcttcagg
     3721 tgcaagacgg cttgcatgga ggtagtgatt gagagcttct gtataagaaa aacgcatagc
     3781 aaaaagatgt gctttgaggc ttgctatacg gcttgaatct tttgtgattt ctgaatgcaa
     3841 tgcaagaaaa ttgtcgagct cattgtagcg tccccacagg tataaaagtg ccgctttttc
     3901 ttcgatgatt tcaattttat tggggaagtg ctcttctgcc ttatgcaaaa gattttctgc
     3961 ctcatcgtag agtttttgtg acatgagcag tcgcgctgca aagacatagt ctgcacgttt
     4021 tgatatacct tgatttaaag ctttttttac ttcgatgagg gcaggaacca ttcgagaaac
     4081 agctgcgtag gagagcgcaa gttgtatacg agcatcactg gggatatagg cattttcgat
     4141 gaggtcgctt gcaaaggctg aaacttgcat ttggcgagat tcaagggaga tgtttagaag
     4201 gcttgcttga cttacttctt cgcttgtcca gttttgtccg gcaaaggcta tgtagccctc
     4261 gctttgcaca gtgtcgtcat cgagcatgag ggcgagtcgt ccatttatta tggctgctat
     4321 ttttgtttca taggcaagaa ttgtcagagg gatttttccg agtgattttt ttgtttcttt
     4381 agtttctgat tctttatttt tatttaaact tcgtgggggg ggtgggcatt ttgtccaacc
     4441 gatgagagga aactggttcc cgttaaaata ggcatcgagg cgaaaaaatc cttggttcga
     4501 tatgagaaaa gaaagatggt ttgcttcatc gacatagcga aataaaaagc caaatgcagc
     4561 ggaaccagct tgggcatctt ggctcagttt atttgcaaga cgcttactta gatttgtttt
     4621 tgaaaagtca atcgtccctt ggaggatgag gtttttgtat tggaactgtg ggttcactgt
     4681 ccatgcgtag aggttttttc gcaaaagctg taattctaaa cccttgttaa actgtgtttt
     4741 atatccgtcg ccttcttctt cgataaatcg ccgcgtttct gggtttgaaa aagaactcgc
     4801 ccaattttca aaggcgcttt gttcaaagtc ttggactgct actttatttt tagatttgaa
     4861 aacacgtttt aaaaatgaaa acatacaaag attttatcat tattagttaa agtgtgcaat
     4921 ctaattaagg cgcaaaaccg atgcaacacg tccttcttct gtgtatgccc gttctggatt
     4981 tttttcatcg acaaaggaag tgaaaccctt ataataagta taataatgcg ttcccggtgg
     5041 caaatgcaaa cgaagttcat aaaagccttg ttttgtttct tccatttgat agatataggg
     5101 gtcccagcct gtaaaacttc ccgaaagacc tatgatttgg ccaggttcac cttcatagat
     5161 aaagcgtaca aaaccatctt cagttttctt tgtaataact ggagcttcgt agggtaaatc
     5221 gacatatgaa agtttcattt gcgttgtttc atcgatgcgt atagctgagt tgagagggtc
     5281 atttgtccaa agcccatcga ttatcaaccg atattgtatg cgttttgtat tttgtggtat
     5341 tttacaaaga taaaataaaa cagaatcgag cggttcatag tccatatcaa aaaacggaag
     5401 gcgttcaaaa gcatggattt ttttatactg ttcaaaatca aagcttatac caacaaaacg
     5461 atattcttct tttgcggtaa agacaatata gccgtcttgt ataaaaggct ctcctacgcg
     5521 tcgtatgctt gaaataaggt tttgcctgtc gtagagctct tgttctgtat acaaagaccc
     5581 agaaaaagca ctaaaaagga gatatataaa aataaagcta ataaaaaaga tttttttcac
     5641 gattataata tcggagtgtt ttaaaaaaaa ctaaaccata aaattttact ataacagcta
     5701 cttgttatat cgataacagt gatgtaaaat ggaaagagta tgccgggatt agaacaacta
     5761 caaaaatttt ctcaagatat aacagggctt ggagacgagc ctgccattcg ggaagagcgg
     5821 ggcgagcctt tttctgtttt gcccatacct cccgatgtct ccgaaaataa cgattcagat
     5881 gattttttac tcggcatgcc tcaagaaggt gaagataata gccttttgga tctagatgaa
     5941 acagaagagc ttggtggcat cgatgatgga gagcaagatt tagccgattt tgcatcgagt
     6001 tttgacttgc ctgaccttga tcccagtgcc gattttgaag atgttttttt atctaaggat
     6061 actacagaac aaactgaagc tcttgatgag acagtgaaat cccttgatga ggacatagag
     6121 gttcttggga aagatttgga aaatgcagac gccttgtctg atctcgcagc ctttgttcca
     6181 gatgttacta ttgacgagga tttagaaagt tttgaggaaa atgtagatga ccttgatgag
     6241 accttcgaag atttagatac actgcctgat attgatgcgc tttctgaaat agaatcggat
     6301 actgaagaac ttatagatgt tgaaaacgaa gaagataaat ctgtccttga tattttagga
     6361 gaatttgaaa aggaaaattt ggctaaagaa acgcctgagg tggaagtctt agatgagttt
     6421 gaagatttat ctgagacttc tgataacctt tctgatttcg atatgctttc tacaacagaa
     6481 gaaccaacta aagccgaaac agcagatgga agcgatgacc ttgaatccct tgctatggat
     6541 ttatcagggg attttttaga gcctgataca ccggggcaac tcgtaccaga ttattctgat
     6601 tttgatgtta caacggatac tgctcaggat atggatgcgc cgatcgagga ttttggggac
     6661 tttgacattg acaatttacc tcctcttgat tctgcctctt tatcctcatt gaatgatttt
     6721 gaaacaacgg atggtattct ttcccaagaa gaagaggtct tagatgatga ttttcatatt
     6781 ccaggatttt cagattttca actagacgga gaaggaaggg aagtagaaaa aaagacttca
     6841 ggtattattc ccggaatttc tgaagcagaa agcagaacaa gtttaagtga agcagaatat
     6901 aaaacattta aagaaaactt acagcactat cctttaaatc taaaaatagc gattcaagaa
     6961 gttatcgtaa aaaatgagtt cactgatgat gcaatttttg gcttgataga aaaagtctta
     7021 caaaagagta ctgctcgcca agtttcttca tttatgtcaa agtatctcga tatatctgtc
     7081 gatgttccga gagattttga aaaaaggtca gttgcacaat atgctgcata taaacagtct
     7141 ttagaatatc aactaaaaaa ccgccttgta ccagcactta ttattggtac tgcggcagct
     7201 cttttattgg gagcggctag ttttctcatc cataactttg tgtataagcc cataaaagca
     7261 gaaggacttt acaaagaagg ctacgcactc ttagaagaaa atttatatgt gcagtcggaa
     7321 gaattgttta atgaggcggt ctattataat ccagtaaaaa aatggttttt tacctttgca
     7381 gaaggctatc gtgaaaaaaa acaatatgag cgaagtcgca caatgtataa tcgcatattg
     7441 gctcgatata aaaatgacaa gaaagcaggt atgtcgcttg ccgaaatgga gctttatgat
     7501 ttagcaaact attcgcaagc agaagaagtg ataaagcgaa aggttttgga ttatcacgta
     7561 aacgataagg atgcgatgct cttactcggt gatgtatacc tagaatgggc tacagaaaaa
     7621 gacccggcgc tctttcctca agcttacgat cagtatatgg acctcataga cttatggggt
     7681 agaaaggacg aatacttaaa acgtctttta cgctattata tccgtatcga taatttgcga
     7741 gaagtgcttc ctcttaaagc atattttctc ccaaagaaaa aacaacttga atctcaagac
     7801 ctcatagaat tgagtggcta tctttttgat aagctctacg gagatttgcc cccacgcgat
     7861 gagtttttac gcagtgaaat agaagatgtg cgtcttttat tggaacgtgc tctggaagca
     7921 gatccatctg ttcccgaggc acattataac tttgcacggt attttctaga aagtaaaaac
     7981 ttcagtaatg caaaaagttt tttgaaatct gcattagatt tatttgaaat ggcagatgag
     8041 cgtaaggcaa agcgtatctt aaaacaaatt catagttata gcttactcgg tgagctctat
     8101 gttgaggagc aagaattttt gcttgctgaa gaaagttatg ccaagggtat acgcttgttt
     8161 gaaaaagaaa aagagatgag cttcttggaa ccaaataagg atgtaggaaa aatatatgca
     8221 aaccttgccg atattgatta ttttatttca ggagatttag catacgcaaa acaaaactat
     8281 gaaaatgcca tagcgaatac ctatgataac gcttctgtgc gttataaaat cggctttatt
     8341 cagtatacaa ataaaaaata tgccgaagct ttgggttcgt atataaaggc aagtgatgaa
     8401 aaacctgaag actatcacct tttactcgcc ttgggaaata gcttgtttta tcgcggcgat
     8461 atctttgcat ctcaagccta ttatgagcgc ttacttgacc tagtcgattt acagcggact
     8521 aggcttggca ttatgtttcc gcaagtgaga accgaccatg cagatttagt tagtatgtac
     8581 ctcaacgctt caaataactt gggagttgtg ctttcaaaac gtgcaaaaag tacaggagat
     8641 tcaaacctta acgccgatgc gatgctttat ttaactgaat ccttgcgtgc ctgggatgct
     8701 ttgacgcgaa atcaagagac aatgatacga cttgaaggaa gtaatcttgc acaacaaaat
     8761 ataaaatacc tcacaaatcc tttaagtgac tacgatgtat ctatttattc aggaattccg
     8821 agaaccttat acggtgagaa aaacattgaa caaagctacc ttaaataaaa tgcaagagaa
     8881 ttttccgcat catcaaacaa agatggatgg tgcgcttgtc caacaaaaga aaacagagct
     8941 tataaaagaa ttgtttcgta aagcaattca catgcttact gtttttattc cctttctact
     9001 gcgcattgcc tactatcccg ttattttact tttgactctt gtcttaattg tctacgtgct
     9061 ttcagaaatc ttacgacttc ataaaaaaga atttcctatt atatctcata taacgagtgc
     9121 tgcggcaaga caacgcgacg ggcatggatt tgttttagga ccggtaatgc ttgcctttgg
     9181 tgttattctt accgctcttt tctttgatcc tctcccagca caaataggca taacagccct
     9241 tgctcttgga gatgggttgg caagtcttgt aggaaagctt tgtggaaaaa tctgtatccc
     9301 atttacaaag gggaaaacac tcatggggag cctcagttgt tttatagcaa tttttctttc
     9361 gacactctgt attacacgga gtttggcacc gagccttatc atcgcctttg ccggaaccct
     9421 tatagaactc attcccatta aagatgtaga taatatcctt attcccctgt ctttggcctt
     9481 attggcacat ttcataatct aagtattttc gtttatttcc atagataata ggcatgaagc
     9541 gttttcaaga aaaaagaatt cccaatacat aacaagagac ttccaagtat acagagtgca
     9601 taggaatctg cgctacccaa aataattgaa ccgattatga gaagcaggta ggaaaaagca
     9661 aataaaaaaa tctttgtttc atagtgctct tttccacgaa tgacaagggc aatagcggta
     9721 atacttatga ggctgtattt tagaactgaa aaaagctggg gaaaacctat tttatagata
     9781 aacgatgctt cgagtatttg tgtgtttata ggcataaaaa agctaaggat aacagcaacg
     9841 acgatgatta tgagggcatt tttgtctgct tgctggagcg ggtcttcaag tgaataaaat
     9901 ggaataaacc aaagagaaag cactgaaagt aattggccag aaaaaaaagt gcgtgataaa
     9961 agtataagaa tagaacggta gccttcgtag agattaaaaa gtggcacaaa gatgcgtagg
    10021 ctttgtaaaa aaatacctgt taaaaaaagt gtaaaataga taagctctgc ggcctgtgtt
    10081 ttttcaaagt tacgtaaaag gaaaaaccca gtgagcggaa gatagagcaa gagaataaat
    10141 aacgaaagaa tacttgcaaa aaaagagctt tgaaaaaagg gcagcgaaaa atgatttttt
    10201 gcaatactta tttcaaaaaa atgagcaggc tcttggttac gaaaaaaaag agcgacaaat
    10261 aaagcgagtg aaaggacaaa aaggagggcg cttgtatagg aaaatatatg caatagacga
    10321 tttcttgaac gcagtgtcat gtaaatagca tactcctaac ttgcatttta gtaaagtgaa
    10381 atataaaaag agccgataaa aagagtaata gcatttttga gaggttcgaa tgaatacaat
    10441 acaggataaa atagcaaaaa aattttttct agcaagtatc tgcatcttgt gtactgcttt
    10501 ttttcttttt gcaggtgatg ttgctgaatt taaagatctc ggtttttcag aagatggaac
    10561 aaagtacagc tttgcacaat acggcataga agataaaagt tttcaagcct gggctgaaat
    10621 atatgttgtc gatattccat caaataattt tgtaaaaaac ggtgttttta gtacaaaagc
    10681 gagcgtacaa acagcttcga aaacaagcat gacggtcttt caagaattgt atgataaaaa
    10741 taaagctttt atcgagcggt atgcaaaaaa gcgggcctca atcgacaaaa ctctgtatat
    10801 tcgggcaaat gaatcaaagg atgctggcga aataatacga tttaaggact atgaaaaaat
    10861 aacaagccag gatgatgtga cttatagctt gcaaagaata atacataaaa cgggaagtgg
    10921 cgaaaaaacc gaatcttcgt tttatattaa cttagaaaaa atagatgcaa atggagttgt
    10981 tattgcgaca cagcttatag ggacaccgca gctaaaaaga aaaggcgttt tagattatac
    11041 gatagaacgt gtttttgtcg atgatagtgg gaaaaacctt attataattg tagaaaaaaa
    11101 gatctatgac ccacgcggca gctctattcg ctatatggta gaagcagcag agcttaaata
    11161 gttccttttg tttcaatagt gcgcaagatt tgaaagagca tttttccacc ttcttcgaga
    11221 atgcgcagat aaaaaaggtc atctacaaag cgaatatttt gccataaggc ttcaagatag
    11281 atagcttcag cagctttttg aaaggctgtt tcatttggct cttgcgcttg tacaaaaaaa
    11341 tagcttgcct tggcaagact aaaatcgcct gtatcaaatg tcctatatcc taaaaacttt
    11401 ttttctctta aaaaatcctc aggctttggc tcgaggtttt ttttatcaac cgtagtttta
    11461 tagacataga gtttttcgct tctgtcgatt gtactgtttt tttctaagtc ctcggatata
    11521 gactcatccg catcacaaaa ataatataaa tcttcaagta aatgtagttt tcgcacaaga
    11581 aatgccattg cttcctctct tttgctttgt tttttacgaa tctcttaagg tttttatgga
    11641 aaataaataa aaataataaa aaacacaaaa aagtccttca aagcgacaat aataatagta
    11701 ggatacgctt tttttgcgtt gatatcaata tgaaaatctg gaggatgtat gaacaaatgg
    11761 atttctttta tggtattttt tgccttactt attctgtcga cactgatact ttttgtgccg
    11821 tcttgtaaat tgagccccca aggtattgtc tttttacatg gcgattttgc gtcacctgtt
    11881 ttattggaaa caaggataca aagtactcaa acaattgatt ttgttttttc agaggctttg
    11941 tcaaagttta gtgctgagtt aaaagaaaaa aaaacagggg aggttttaga ttcacgcttt
    12001 attatgagag aggaagatgg gcgttttatg gtgagtctgc agctcgaaga ggggacaaaa
    12061 gcaggtgagg gctatgaatt gcatgcacgc atttttgatg tagcgggtaa ttcttgtgat
    12121 attctttttg agttttcagg tttcaatgat agaattccac aagtgattat aaatgaaatt
    12181 caaacggagt attcgaaacc aaaggcagag tttatagaat tgtactgtca aacaagtgga
    12241 aatatcgggg gaatgtatct gtataatgca ggggatggtg aagcgcttgc atataccttc
    12301 ccagccgtag aagtttcagc aggtgagtac attgtcttgc attatcgcag tttgcctgag
    12361 gaagatattg tcgatgagct ttttgccttg gatgagtcgg gtggaaccta ttctcaccca
    12421 gaggcacgcg atttttgggt agcggaaaac aaggcacgac ttccaaaaag cgatgttatc
    12481 ttgctcaaaa gctcttttat gggagaaatt atcgatgcgg tgatgtatag cgaaagcgga
    12541 aaaacagaat ggaagacaga tgcgctaaaa gccgcagcaa gagcagttgt tgaagcaggc
    12601 ttttatacga gtgattcacc tgtggatctt ccgtgcagcg acaaagttac tgttacgagg
    12661 acattgagcc gtcaaagtga tggaacatgg tttgtcacaa agacgagtgg tgcaacgcca
    12721 ggttatgaaa attgtactgt tccatattga taggtctttc tagtccctga ctatattaaa
    12781 atctttgttt ttgctatagt taggctatgg cagaattact cgctcctgca ggcaatatag
    12841 aagcactgaa cgcagcaatt ggcgaaggtg cagatgcagt atacatggga ttaaagagct
    12901 ttaacgctcg cttacgcaca acaaactttg cgtggaatca gtttgaggcc gcagtcgaag
    12961 cggtgcataa acgtgggaag aaaatttttg taacggtgaa cacccttgtc gaagaaggcg
    13021 agcttgaacg gctttatcgt tttttagaat atctacagcg agtaggtcct gatgggctca
    13081 ttgtccaaga catgggtacc attcgaatga tacaagaatt tttccccgat ttgcgcatac
    13141 atgcttcgac gcagttaaac gcatcaagtg cacatgcggc aaatcttctt gcaaaggaag
    13201 gtgtaaagcg tgttgtgctt gcgcgcgagc tgggttttag tgaaatacaa gcgattcgac
    13261 aaaaaacaaa tatcgaactg gaagtatttg tgcacggtgc gctttgtgta agtgagtcag
    13321 gactctgtat gttttcgagc tttttgggtg gaaaatctgc aaaccgtggt atgtgtacac
    13381 aagcgtgtag acgtttttac ggggcggaaa agccttctgg gcaagaacaa ggctattatt
    13441 tttctcccta cgacctacaa cttatcgatt atatccccga tttggtgcaa gcgggtgtag
    13501 agtcctttaa gattgaagga cgtatgaaaa gcgctgagta tgttggtact gttactgctg
    13561 cctatcgtta tgttttagat cattggcaag aggataaaaa agctgcgatt gaaacagcga
    13621 agcgtatttt ggcaacggac tttgcacgta aaaagacaag ctattggtat gaatcttcga
    13681 gcgcagaaaa ccttctcgat ccaaagcagg cggggggaac agggatttat ctcggaaaaa
    13741 tcgaaaaaat ccgctaccat gaaaaaacaa aacagccata cattttttta cgtggtggct
    13801 cctatgaacc agaaataggg gattctatcc gtttgcataa aaaagatgat tcaaatcgtg
    13861 tcagtcataa ggtaaaagaa atccttgaat acgctggtga tttatttata gatgctccca
    13921 agggttttag taacggcgat acagtgtact tgttgcaaac aaagtctatg tcaaagcgcc
    13981 atccctttgt tttgccaaaa gatttgagca agtatcgaag acaacctggg gcaggtacct
    14041 tgccgcttat ggagttagag cgtcttccta aaaatagctt agactatttt cctcaagggc
    14101 tctatgtgca ggtttccaca gtagaagatt ttcatgcagt tttacatcaa aataatgtgc
    14161 gctatatctt agagtttaat tttaaaacaa aagaagcgct tatagataat atggaatcgc
    14221 taccggtttc aaaaaaacaa gttattatat ccttggaccc tttttacccg caagatttag
    14281 aagaaagctt gcagcaagat atggacgatt tagtagggct tggctatgaa agtttcatgg
    14341 taaataatct cggacacttt gctttatttg acagaaaaaa aacaaatctc attgctggtc
    14401 cctacttgta tacgatgaat cgttgggcag cttcttttct cgaaaatcaa aatattgaag
    14461 cctttgtttc atcctatgaa aactcgagaa aaaatcttga ggcaagtttt gaacaagtgc
    14521 atcggaaaaa aatcttactt ccactttttg cctatcctgc actatttcgt atgcgtttta
    14581 ctctaccaga atcctatgac tttaattatt ttactgacaa ggaaggcgaa atgttcaaat
    14641 cgctttcaac accagatggc tcctttgtgc ttcccgaaaa acctttttct attttagata
    14701 aatatagtca aatgcagtcc gcaggtttta gtcgtttttt aattgatctt tcaaaaacaa
    14761 aggtagcaaa ggggacttat aagcaacttt tacgatcgat gtatacctct gaacccttag
    14821 aagatattag ccgatttaat tgggctgaag gtttttatga tccagaaaag atagcacagt
    14881 ataaaaaagc caatcttaat cgcgaaactt tttctaagtc gagacctaga aga
//
LOCUS       DAOSSW010000042        14768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_4149_length_14768_cov_7.059947, whole genome shotgun sequence.
ACCESSION   DAOSSW010000042 DAOSSW010000000
VERSION     DAOSSW010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 14768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14768
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4149_length_14768_cov_7.059947"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..136)
                     /locus_tag="PKW26_04945"
     tRNA            complement(64..136)
                     /locus_tag="PKW26_04945"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(101..103),aa:Asn,seq:gtt)
     gene            complement(195..776)
                     /locus_tag="PKW26_04950"
     CDS             complement(195..776)
                     /locus_tag="PKW26_04950"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOQ92945.1"
                     /translation="MRKKTKQELRNFLKSKNLERGKTSANIKKIPRYKKAHTIFLFLS
                     LKDEIDTKPLLEQALKDGKKIALPKILSTKTNEMSFFYLDKQFPIDMQIEKGAYEIFE
                     PKTSLEEAKQEELTKNDLVFFPGLAFSKDGKRLGRGKGFYDRYFFDLWKNPNRPQFIG
                     LCYENQIVNTIPTDSYDISMDALLTEKKYYICR"
     gene            complement(804..3485)
                     /locus_tag="PKW26_04955"
     CDS             complement(804..3485)
                     /locus_tag="PKW26_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92946.1"
                     /translation="MFSLFNTIRSQRKKIVFCISLCFAFLSILGTLEAQERQNNSFLL
                     ENGLEIEILERNKDPIAHIEIELGSGYGATEKNEAGFFDLSRRLFWQNSESAKEIEKT
                     LGLFDLETNLFAQNTVFSASLPAHSLEQSLIYIKECIENSLFAEKSLVAVLDEYLQEQ
                     KAILASPYGFIHASVMNHLYPENPWAQSADMGYYYFQEKKIESLALALKLFKERYYQV
                     QNIKLRITSPYETSLVLALVKKSMQNWENSSSYLPKRKLSKLQKEQKSLVLVSEGFPE
                     DFEQFIFIRTIDIGDSHLWEDKTEETIHREAAFLSFLLEENVDAIKTKFLEEKSLGLE
                     DEQYLAFSFEKAADYSRLISQYMTKKNTTSLLDKIKTIQEQENKISFSRESIEKMLPY
                     FEFYEQAEKNQSQTDTFFLQDGFSPFSQIEPIHIILLHPKTYAKHKKIIENEKWLVKT
                     EKDFQEDIKKILAQKIEVLDESFSALPPYEAIFDDMNDSLRLKLSNDIAVFMRIDSKA
                     EYTEIDFNFQKNAESADGKTYLIDEIIFRSLEKKYRELNLEKMLKGEQGEVLFYSDRG
                     KNKLRFTIKAKAEDIEKAFYDFISLFTFFSISPLEADIVNKEIQGEERIKTSLLKTQL
                     DGVALQEIFDKTKQPPLFSINTDAIRYEDIQKRLSKLYNAQAVSLAFMGREESLVNLY
                     VLSEKTLSFLKNQGKISTEVYSFEIPDRKKRVSLSHLFTSKLDAKDAGPRPLRLIPTT
                     EFFDPLHIYFKRPLDENQAYFDAFFYTLSAILNETNTEKSFSNLQYYIDTEIPELASL
                     EFSEVKTAKKIESLFSKTLASFPEWIEDESALERIKNIWIKEKFTQYETKSIDEKIQM
                     QKDLLKIIKAESSVFLEVFELLIKQAPKLWLISQDTK"
     gene            3599..4075
                     /locus_tag="PKW26_04960"
     CDS             3599..4075
                     /locus_tag="PKW26_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOQ92947.1"
                     /translation="MALGDAQFQLVTFQLGEELYGVDIMDVKEIVKVQTVRPIPNAPY
                     YVEGIFNLRGEIIPIINLHKRFRLKNIEIAEEDAFERGFIILSIDGSKIGIIIDRVAR
                     VVLVQNEEVKPPPQMLAGIGTEYVHGVIRQDAGYLILLDTRKIFNPKELQKLIGMQ"
     gene            4078..5106
                     /locus_tag="PKW26_04965"
     CDS             4078..5106
                     /locus_tag="PKW26_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOQ92948.1"
                     /translation="MKIPTFSSTIRRKEMDSVLTTLVDEKIGPGEAVIRLIQQVKAFF
                     GVDGAVALRSPSLALKYALKALDIPKGSKVIVSALAPYWQYVCLEEEGYEPLVIDVSP
                     ETALLSPETVLEAVKEGGSLLILHAAMGFVPNIQALLDIGLPIIEDISQSAGASLNEK
                     MLGTFGVFSILGLEEEDSITGGGGAVLMAPERRNWIVLKKHSDEALSTDILPDINASL
                     ALVQLKEFNKNEQIRKEMYEIYARSIMQGKHKTFLQAGDYAIPTVYSFPVILSSGFKD
                     VRQYALRKDIEIAPVFAQSIIAYKEEELSDLIHAKSFLLRCAYFPLYPRLGAQQAAKI
                     AKVLATLP"
     gene            5157..6005
                     /locus_tag="PKW26_04970"
     CDS             5157..6005
                     /locus_tag="PKW26_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOQ92949.1"
                     /translation="MKACIIVVNTYNEESLMLSKEIFSFLKKTGIQATIINYGEEDAE
                     HPNIPENIDFAVSLGGDGTVLYTARLCAKKAIPIFPVNLGEFGFIAGIQKNDWEKPLQ
                     AFLEGSFLPLKRSMIEVLLFRNQECVFSEIALNDFVISASGAARIVQLEIEANEVPFG
                     VFKADGIIVSTATGSTAYSAAAGGPIVDPALDALILSPVCAFSLSNRPLVLPATASLS
                     IRVLSSRDTDVMLTCDGQVGFQLAVDDVLLIKKAEVSAHLLGCDANVFYSALRTKLHW
                     SGGPRA"
     gene            5998..7692
                     /gene="recN"
                     /locus_tag="PKW26_04975"
     CDS             5998..7692
                     /gene="recN"
                     /locus_tag="PKW26_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOQ92950.1"
                     /translation="MLEDISIKDFVLIDSLSLDFSKGLTVFSGETGAGKSILIGAISF
                     LLGGKGGVELVRDGASEARVSGSLYISPEHIEARQWLSEHHIELDENRLLLRRVLRSN
                     GKSASWIQEAQVTKAEVQEFTALLVDIHGQHEHQSLMRPSEHRKFLDAYAGIENKVRD
                     FTQLYTDLITKRQKMQAVQTSDAERKRKIEMLAFACEEIEALHIRVNEETELEEELSR
                     LSQFEKLYETIQDMNQTLSTGDSALVSHLKKLHVSASHIEDLDKVLATLSQRLESAFY
                     EVSDIANELNAYQQSLIFDPSRLEEVQERLAQFFRLKKKYLKNQNEKADALLDFLKKA
                     EKELLDLQNWEEDRELLEKEIKDLEKKLYDAAVFLTEKRRAFSEKMSEQLVIILQDLG
                     MKNTLFQVSISEKESTEFIQKCGPYGKDNIEFLIAANEGSVLRPLSKIASGGEISRVM
                     LALKTIFSATDAIESLIFDEIDTGIGGEIAVSVGKHLKALSKKKQVFCITHLASIAVF
                     ADTHMRIEKKTDKGATFTSVVPIMGEDRVEEIARMLSGDAFSQASIDHARSLLAMS"
     gene            7709..8512
                     /locus_tag="PKW26_04980"
     CDS             7709..8512
                     /locus_tag="PKW26_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92951.1"
                     /translation="MAKIAAESRELFNSKSKSFRDLIQKSLDKEKSMISLMKSDNSGL
                     AYKKLLLAEEMIYVTTLYLTINNLSVEILGPKNTDALNEGRKTLYKAIIYLEEIVTNL
                     IDAPFSEYEDDLKEIQNIPLSKRYLVVRKLGLAIDLILEGFGDNTKWKWSFTELKGRF
                     ATVAKNFLDLKVAMQDHFNPRSADCDTTVMYVRLIKRLLNESADGYRERYELSTRRID
                     DMRLAIQYLLALRRIHLLLNEKDEAEDIKKKAIVWKEKMEADQKKGTSK"
     gene            8513..9718
                     /locus_tag="PKW26_04985"
     CDS             8513..9718
                     /locus_tag="PKW26_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ92952.1"
                     /translation="MLTKEIALKLFEAFSIERWNDLVRPFELNEMDKSAEKMVLAYII
                     GKYEENRGGEVDWDWIIHASLFDLLRKIALCDIKSPVQRMIRSKYPSEYKKLNEWVIG
                     QYEELLKNTGLIEAFRDYLFNEKPDSFLSKRILRCAHKYSAMREFEMISVVNEPFRVN
                     HIQQALLKDIEVYLDLRGLQLLIAKQKPYQFLMIIEQLRFQTRWNQTPRIPKTSVLGH
                     SLYVALLTFFISKGILSMHPKRLYNNFFSALFHDLPEAVTRDIISPVKQATEGLPSIV
                     KEIEEDVLQSDLVPLMEDFYVDELLYFTSDEFSNRIAIDSRLKKTQKLSFEEINTLHN
                     KADTTAIDGELVRLADHAAAFIEADRSIKFGISSEQLKEGRDNIRNLYPAGKIQSAFP
                     IGDFYAQFL"
     gene            9753..10574
                     /locus_tag="PKW26_04990"
     CDS             9753..10574
                     /locus_tag="PKW26_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOQ92953.1"
                     /translation="MKKFLRLAFLILFFSLSLSFFAQAKEHTVAKGETLYSISRSYNL
                     TVEELCSLNNIKQSEVLKIGQVLLVSKAIKIPQETETYVVQAGDTMYSIARRLSVSVE
                     TLNILNQLSNASLIKVGQVLTVPSTTSIQPDISQSASDLSLIDPRSYDTKKPVNKSLL
                     WPVTALDIHYLSGKISGVSIMTNEKEAVKAVQEGVVMYTGPYRGFGQVVFVQATSKHM
                     YVYTGLESIIVQNGQSIRKGDRLGIVGIDALSGKSALTFMVFLNGNAIDPAKAPR"
     assembly_gap    10592..10691
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10692..12227)
                     /locus_tag="PKW26_04995"
     CDS             complement(<10692..12227)
                     /locus_tag="PKW26_04995"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOQ92954.1"
                     /translation="MEKEAIIQKANEYLQEERDSYFKSEIEKLLKEENYIELEDRFYQ
                     SLEFGTGGLRGIIGGGTNRINTHVIHKVTQGLANYCIKVLPEKAKKKELKAVIAYDSR
                     RFSDVFAEATALIFAANGFTTYLFSSLRPTPELSFAIRELACDTGVVVTASHNPPEYN
                     GYKAYWNDGSQVIEPHDVGIIEEVNTVSDIKYITRVEAIANGSLNIIDAEIDEPYWAM
                     VKSKLFRPKLIKEKAKEVKIVYTPLHGTGAMHVEKVLGDLGLEVITVPEQREPDGNFP
                     TLEKPNPEEAAALKMAIDLAKKEKADIVMATDPDADRFGIAVPGKDADFVLITGNQIG
                     VLLADYIIVSRKELGLMPKNPAIIRSIVTSSFVDRIAKANDVYLAECLTGFKWIASVM
                     SDFEKTKSHNYIFGFEESYGYNIETEVRDKDGISAAALCAEMTLYWRSKGISILDRLE
                     QLYETYGYHEDFAISKTFLGSSGAQQMKSLMDMLRKDGLKEIAGVKVEKIRDIGESIE
                     YNPQ"
     gene            complement(12284..13774)
                     /locus_tag="PKW26_05000"
     CDS             complement(12284..13774)
                     /locus_tag="PKW26_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOQ92955.1"
                     /translation="MSESLNKNYTRDSIIGLGEESFKKNYYPELLQKITMLEKLQNRN
                     ETLINAIRDILFIYDEKAEIKPFSSTRIEPHKLMSPILANAEAMENLKMGAEQALKHA
                     SYEITFTLENKEKLMHLEARFNKTQSNEVIIIVRDISDIVELQKIHSSLLNLDPISKL
                     YNRRWFDSKINEFTKVTAKHMALFILEVTGLQFIKTTLGQIHSEQLIVLAGEIISVFF
                     SENTTIARISFESFAVIIEDISEMETEALAKNFETFMAKHNNSTNTKNVLISYGYAHH
                     SEGIINMETMFVNANSFLHLSTLTQYERAKRFYLKRFLKTIEHKISNREASKECKKVL
                     VNEFSRLLNLTSYKRKKLHQLSRLYDIGEIGLPEHIFQKKESLSEMEWSIVRSHTMIG
                     EKLLTEFPEYKGLANFVLYHHENWDGSGYPYGLFKEAIPLESRIIRIIDSIVAMAHDL
                     PYRKKIACEQLIKELEKGAGREYDPNLVSLILPFLDKNKALIKSCP"
     gene            complement(13755..>14768)
                     /locus_tag="PKW26_05005"
     CDS             complement(13755..>14768)
                     /locus_tag="PKW26_05005"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOQ92956.1"
                     /translation="FADIIERIKKQVSEFIIFSDVSENPKDIEVHKGLTAYINNNCDV
                     IVTIGGGSPIDCAKGIAIMATNTGNILDYEGVDMVKTPGPPLCCIPTTSGASADVSQF
                     AIINDTKRSLKIAIISKKLIPDIALIDPVPLMTMDPYLTACTGMDALTHAIEAYVSNA
                     SSAITDTHALEAIRLISETIEKAITKERTIEDMHTMMMASTHAGFAFSNASLGAVHAL
                     AHSLGGLLGLPHGECNSILLEHVIDYNFDAEPERYKNIALKMKLEVNNLNHDEVKKIL
                     IEKIQELRNALGIPAKKTIPTKSENLLSQLAQNAYEDPCLVTNPKTMTIETMKELYER
                     ILE"
BASE COUNT         4264 a         2595 c         2973 g         4836 t
ORIGIN      
        1 ttcaagccca gattgtagaa tataaaaaaa ggactgatca agcgaccagt cctttttttg
       61 acgctccccc ggctgggctt gaaccagcga cccacagatt aacagtctgt tgctctgcct
      121 gctgagctac aggggaatgt agtattgata gtataagaat tagaaaaaaa agtcaatata
      181 ctagtgtgtt ttttttacct acagatataa tatttttttt cggtgaggag ggcatccatg
      241 gaaatatcat aactatctgt tgggattgtg ttgacaattt ggttttcgta acagaggcct
      301 ataaattgtg gacggtttgg gtttttccat aagtcaaaaa agtatcggtc ataaaagcct
      361 ttccctcgtc ctaatctttt tccatccttg ctaaaggcga gcccagggaa aaaaacaagg
      421 tcattttttg ttaattcctc ttgttttgct tcttctaagc tcgttttggg ttcaaatatt
      481 tcgtaagctc ctttttctat ttgcatatca atgggaaatt gcttgtccag ataaaaaaaa
      541 ctcatttcat ttgtttttgt agaaagtatt tttgggaggg cgattttttt cccatcttta
      601 agggcttgct ctagcagggg ttttgtatcg atttcgtctt tgagagagag aaataaaaaa
      661 atagtgtgcg cttttttata tcttgggatt ttttttatat tggcacttgt ctttcctcgt
      721 tctaggtttt tacttttgag aaaatttcgt aattcttgtt ttgtcttttt tctcatcgct
      781 tttttattat agatttgcat ggcttatttt gtgtcttggg atatgagcca gagcttcggt
      841 gcttgtttta tgagcaattc aaaaacttcc aaaaaaacac tgctttcagc ctttattatt
      901 tttagtaaat ccttttgcat ttggattttt tcatctattg attttgtttc atactgcgta
      961 aatttttctt ttatccagat attttttatg cgctccagtg cgctttcgtc ttctatccat
     1021 tctgggaagg aagcgagtgt ttttgaaaac aagctttcta ttttttttgc ggttttaact
     1081 tcggaaaatt ccaagcttgc aagttctggt atttctgtat caatataata ctgcaggttt
     1141 gaaaaacttt tctcagtatt tgtttcattg agtattgcag agagggtata aaaaaatgca
     1201 tcaaaatacg cttggttttc gtcgaggggt cttttaaaat aaatatgcag cgggtcaaaa
     1261 aattctgtcg tcggtataag tctaagcggg cgtggtcctg catctttcgc atcgagcttg
     1321 ctcgtaaaaa gatgggagag tgaaactctt ttttttctat cgggtatttc aaagctatag
     1381 acttctgtgc ttatttttcc ttggtttttt agaaagctaa gtgttttttc agaaaggaca
     1441 tataaattca cgaggctttc ttctcttccc ataaaggcga gagagactgc ctgtgcattg
     1501 tagagttttg aaagtcgttt ttgaatatct tcatatcgta tggcatcggt gtttatagaa
     1561 aaaagagggg gctgttttgt tttatcgaag atttcttgca aggctactcc atctagctgt
     1621 gttttcaaaa gcgatgtttt tatacgttct tcaccttgta tttctttatt gactatgtcg
     1681 gcttcgaggg ggctgattga aaaaaaagta aaaagggaga taaaatcgta aaaggctttt
     1741 tctatatctt ctgcctttgc tttaatcgta aagcgaagct tgttttttcc tctatcagaa
     1801 taaaacagca cttctccttg ctcgcctttg agcatttttt caaggttgag ttctctgtat
     1861 tttttttcaa gagagcgaaa aataatttca tctatgagat aggtttttcc gtctgcactt
     1921 tcggcattct tttgaaagtt aaaatcaatt tctgtatatt ctgctttgga atcgatacgc
     1981 ataaagacag ctatatcgtt gcttagtttt aagcgcagac tatcgttcat atcgtcaaag
     2041 atagcttcgt atggaggaag ggccgaaaag ctttcgtcta gcacctctat tttttgtgca
     2101 agtatttttt ttatatcttc ttgaaagtct ttttcagttt ttacaagcca tttttcgttt
     2161 tctattattt ttttatgctt tgcataggtc ttagggtgca agagaataat atgtatcggt
     2221 tctatttggg aaaatggcga aaagccatct tgtagaaaaa aagtatccgt ctggctttga
     2281 tttttttctg cttgttcata aaactcaaaa tagggaagca ttttttctat tgattcacga
     2341 gaaaaggata ttttattttc ttgttcttgt attgttttta ttttatccaa aaggcttgtt
     2401 gtgttttttt tcgtcatgta ctggctaata aggcgtgaat aatcggcagc tttttcaaag
     2461 gaaaatgcaa ggtattgctc atcttcaaga ccaagagact tttcttctag gaactttgtt
     2521 tttatagcat cgacattttc ttcaaggaga aaactgagaa aggcagcttc tcgatgtatt
     2581 gtctcttctg ttttatcttc ccaaagatga ctgtctccta tatcgatcgt gcgtataaaa
     2641 ataaattgct caaagtcttc aggaaagccc tcggagacaa gcacaaggct tttttgttct
     2701 ttttgcaatt tgctgagctt tctcttgggg agatagctgc tagaattttc ccagttttgc
     2761 atcgattttt ttacaagggc gagcacaagg cttgtctcgt aggggctggt aatgcgcagt
     2821 tttatatttt gcacttgata atagcgttct ttaaatagtt ttaaggcaag ggcaaggctt
     2881 tctatttttt tttcttgaaa ataataatat cccatatctg cagattgtgc ccaagggttt
     2941 tcggggtaga gatggttcat aaccgatgcg tgaataaaac cataggggct tgcaaggatg
     3001 gctttttgtt cttgcaagta ttcatccaaa actgctacta aggacttttc tgcaaaaaga
     3061 ctgttttcta tgcactcttt tatatatata aggctttgct caagactatg cgcagggagg
     3121 gaggcagaaa agacagtgtt ttgggcaaat aagtttgttt ctaagtcgaa taagccgagc
     3181 gttttttcta tttcctttgc gctttctgag ttctgccaaa agaggcgacg actaaggtca
     3241 aaaaatccag cctcattttt ttctgttgcg ccatagccag agcctagttc tatctcgata
     3301 tgtgctatag ggtctttatt tctttcaaga atttctattt ctaagccatt ttcaaggaga
     3361 aaggaattgt tttgcctttc ttgcgcctcg agagttccaa gtattgataa aaatgcaaaa
     3421 caaagagaga tacaaaaaac tatttttttt ctttggctcc ttattgtatt aaatagagaa
     3481 aacatagctt tattatagtc taaaaatgat aaaagtctat tattatagct gaatataata
     3541 gaatgcttgt attttttgaa aattcaatgt atcctatgag ggtattattg gaggtacaat
     3601 ggctttaggt gatgcacaat ttcaattagt tacatttcaa ttgggtgaag agctttacgg
     3661 tgtagatatt atggatgtaa aagaaatcgt aaaggttcaa acagttcgtc ctatcccaaa
     3721 tgccccgtat tatgttgaag ggatatttaa ccttcgcggt gagattattc ccattatcaa
     3781 tttacataaa cggtttcgat tgaaaaatat tgaaattgcc gaggaagatg cgtttgaaag
     3841 aggttttatt atactcagta ttgatggaag taaaatcggt attattatag atagggttgc
     3901 acgtgttgtt ttggtacaaa acgaagaggt aaaaccgcca ccacagatgt tggctgggat
     3961 tggtacggaa tatgttcacg gtgttattcg ccaagatgct ggctacctta ttttacttga
     4021 tacgcgaaaa atctttaacc cgaaagaatt acaaaaactt attggaatgc aatagcaatg
     4081 aaaattccca catttagctc tactattcgc agaaaagaaa tggattctgt gcttaccacc
     4141 ttggttgatg aaaaaattgg accgggggaa gcggtaattc gcttaattca acaagtaaag
     4201 gcttttttcg gtgttgacgg tgcagttgcg cttcgcagtc caagcctcgc tttaaaatat
     4261 gccctcaaag cccttgatat accaaaggga tctaaggtta ttgtttcggc tctcgctccg
     4321 tattggcagt atgtgtgctt ggaggaagag ggctatgaac cgctcgttat tgatgtgtcg
     4381 ccagaaaccg ccttgctttc tccagaaaca gttcttgagg cagtgaaaga gggtggcagc
     4441 ttgctcattt tacatgcggc gatgggcttt gtcccaaaca ttcaagcctt acttgacatt
     4501 gggcttccca tcatcgaaga tatttcccaa agcgcaggtg cgtctcttaa cgaaaaaatg
     4561 ctcggtacct ttggcgtctt ttctattctt ggtttagaag aagaagatag cataacggga
     4621 gggggagggg ctgttctcat ggctccagag cgacgaaatt ggattgtttt gaaaaaacac
     4681 agcgatgaag cgctctcaac cgatatctta cccgatatca atgcatccct tgccttggta
     4741 caattaaaag aattcaataa aaacgagcag atacgtaaag aaatgtatga aatatatgct
     4801 cgttctataa tgcaaggaaa gcataaaacc tttttacaag cgggtgatta tgcgataccg
     4861 acagtttatt cttttccagt gattctttcg agtggcttta aggatgttcg ccagtacgcg
     4921 cttcgaaaag atatcgaaat agcgccagta tttgcgcaat ctatcatagc ctataaagaa
     4981 gaagaattga gtgatttaat acacgcaaaa tcttttttgt tgcgttgtgc ttattttccc
     5041 ctttatccac gtttgggtgc gcaacaagcg gcaaaaattg caaaggtttt ggcaacttta
     5101 ccttagatac aggtaagatg gtatgtaagc tatcaaaaaa agtgaaggaa aacaggatga
     5161 aagcctgcat aattgtagta aatacgtata acgaagaatc tcttatgctt tctaaagaaa
     5221 tattttcttt tttaaaaaaa actggtatac aagcgacaat aataaactat ggggaagaag
     5281 atgctgaaca cccgaatatt cctgaaaata tcgattttgc ggtgagtctt ggaggcgatg
     5341 ggactgtttt atacacagca cgattatgtg caaaaaaggc aatccccatc tttccggtaa
     5401 atttgggtga gtttggtttt attgcaggta ttcaaaaaaa tgattgggaa aagcccttgc
     5461 aggctttttt agaagggagt tttttgcctc ttaaacgaag tatgatagaa gttctgttat
     5521 ttcggaatca agaatgtgtt ttttctgaaa tagcgctaaa cgactttgtg atttctgcat
     5581 ctggtgctgc tcgcattgta caattggaaa tagaggcaaa tgaggttccc ttcggcgtat
     5641 ttaaagctga cggaattatt gtgtcgacag caactggttc aaccgcctat tcggctgcag
     5701 ctggaggacc catcgttgac cccgcactcg atgcgcttat cttaagtcct gtctgtgctt
     5761 tttcgctttc aaatcgtccc ttggttttgc ctgcaacagc gagtttaagc attcgagtgc
     5821 tttcttcaag ggataccgat gtgatgctta cctgcgacgg acaggtggga tttcaacttg
     5881 ctgtcgacga cgtattgctt ataaaaaagg ctgaagtttc ggcacattta ctcggctgcg
     5941 atgcaaatgt gttttattcg gccttacgaa caaagctgca ttggtcagga ggacctcgtg
     6001 cttgaagata ttagtataaa agattttgtt ctcatagatt cactttcctt ggatttttcc
     6061 aagggactga ctgtctttag cggagagaca ggggcaggaa aatccatact tataggggct
     6121 atttcgtttt tacttggtgg taagggagga gtcgagcttg tccgtgacgg tgcaagtgaa
     6181 gctcgtgttt ctggtagtct gtatatttcg cccgaacata tagaggcgcg acagtggctc
     6241 tctgagcatc acatagagct tgatgaaaac cgtttgttgt tacgacgagt tttgcgatca
     6301 aacggaaaat ctgcctcctg gatacaagaa gcacaggtaa caaaggctga ggtgcaagag
     6361 tttaccgctc tcttggtgga tatacatgga caacatgaac accaatctct tatgcgcccg
     6421 tcggagcatc gaaagttttt agatgcctat gctggtattg aaaataaagt gcgtgatttt
     6481 acacagctgt atacagattt aatcacaaaa cggcaaaaga tgcaggctgt tcaaacaagc
     6541 gatgcagaac ggaagcgaaa aatcgaaatg cttgcctttg cctgtgaaga aatcgaagcg
     6601 cttcatattc gtgtcaatga agaaacagaa ctagaagaag agctttctcg cttatcacag
     6661 tttgaaaaat tgtatgagac aatacaggat atgaaccaaa cgctcagcac tggcgattct
     6721 gccctcgtat cgcatttgaa aaaactccat gtttctgcaa gtcatataga agacctcgac
     6781 aaggttcttg cgaccttatc ccaacgtctc gaatcagcct tttacgaagt ttccgatata
     6841 gcaaatgagt tgaatgccta ccaacaatcc cttatctttg atccatcaag actggaagaa
     6901 gtacaagagc ggcttgcaca attttttaga ctaaagaaaa aatatctaaa aaatcaaaat
     6961 gaaaaggccg atgcgctttt ggattttttg aaaaaagcag aaaaagaact tcttgacttg
     7021 caaaactggg aagaagatcg agagctttta gaaaaagaga taaaggattt agagaaaaaa
     7081 ctctacgatg ctgcggtatt tttgactgaa aaacgacgtg cgttttcaga aaaaatgtca
     7141 gaacagctcg ttattatttt gcaggacctc ggtatgaaaa acaccctttt tcaagtatcg
     7201 attagtgaaa aagaaagtac tgagtttata caaaaatgtg gcccctatgg aaaagacaat
     7261 atagaatttt taattgcagc taatgaaggc tctgttttgc gtcctttgag taaaatagca
     7321 tccggtggtg aaatatcgcg tgttatgctc gcattaaaaa caattttttc tgcaacagat
     7381 gcaattgaaa gccttatttt tgatgaaatc gatacgggaa ttggcggaga aatagctgtt
     7441 tctgtcggaa aacaccttaa agcgctttca aaaaagaaac aagttttttg cataacacac
     7501 ctagcgagca tcgctgtttt tgccgatact catatgagaa tagaaaagaa aaccgataaa
     7561 ggtgcgacat ttacgagtgt tgtacctata atgggtgagg atcgtgttga agaaatagca
     7621 cgaatgctct ctggagatgc attttcgcag gcctcaatcg atcatgcgcg ctcattattg
     7681 gcaatgtctt agcagaagag ggaaaaatat ggcaaaaata gcggcagaaa gcagagagtt
     7741 gtttaactcg aaaagtaaat catttcgtga tttaatacaa aaatctttag ataaagaaaa
     7801 aagtatgatc agcttaatga aaagtgataa ttcaggactc gcatataaaa agttgctttt
     7861 ggcagaagag atgatttatg ttactacgct gtatttaact ataaataatc tttcagtgga
     7921 aatccttgga cctaaaaaca ctgatgcttt aaatgagggg agaaaaacac tgtataaggc
     7981 aattatttat ttagaagaga tagttacgaa tcttattgat gctccttttt cagaatacga
     8041 agatgactta aaagagatac aaaatatacc actttcaaaa cgctatcttg ttgttagaaa
     8101 acttggatta gcaattgact tgatcttaga aggttttgga gataatacaa agtggaaatg
     8161 gtcttttacc gaactaaaag gtcggtttgc cactgtagca aaaaattttt tagacttaaa
     8221 agttgctatg caagaccatt ttaatccacg atccgctgat tgtgatacaa cggtaatgta
     8281 tgtacgactt attaaacgct tattaaacga atctgccgac ggttacagag agcgctatga
     8341 gctttctaca cgccgaatcg acgatatgcg acttgctata caatatttgc ttgctttacg
     8401 acgtattcac ttattactca atgaaaaaga tgaggcagaa gatattaaga aaaaagcaat
     8461 agtctggaaa gaaaagatgg aagcagacca aaaaaaagga acaagtaaat aaatgctcac
     8521 aaaagaaata gccctcaagt tatttgaagc tttttccata gaacgttgga atgatttagt
     8581 tcgccccttt gagctcaatg aaatggataa gtctgcagaa aaaatggtcc tcgcgtatat
     8641 cataggaaaa tatgaagaaa atcgaggagg cgaggttgac tgggattgga ttatccatgc
     8701 ctccttattt gatttgttgc gaaaaatagc actctgcgat ataaaatctc ctgtacaacg
     8761 aatgattcgc tcaaaatatc cgagtgaata taaaaaactt aacgaatggg ttatcgggca
     8821 atacgaagag ctactaaaaa atacaggtct catagaagca tttcgcgact atctttttaa
     8881 tgaaaaacca gatagctttt tgtccaagcg tattttacgt tgcgcacata agtattctgc
     8941 aatgcgcgag tttgaaatga taagcgtagt gaatgagcct tttcgcgtta atcatattca
     9001 acaagcccta ctaaaggata tagaagtgta tttggattta cggggcttac aacttttaat
     9061 cgcaaagcaa aaaccctatc agtttttaat gataatcgaa caactgcgtt ttcagacaag
     9121 gtggaatcaa acaccacgta taccaaaaac ctcggtttta ggacattcgc tctatgttgc
     9181 gcttttaact ttttttattt caaagggtat tctttcgatg catcccaaac gcttatacaa
     9241 taattttttc tccgctcttt ttcacgactt acctgaagca gtcactcgag atattatttc
     9301 gcctgtaaaa caagcaacag aaggtctccc atccatcgta aaagaaatag aagaagatgt
     9361 ccttcagtct gacttggtgc cactcatgga agatttctat gtagatgaac ttttatattt
     9421 taccagtgat gaattttcaa atagaatagc aatcgactcg cgcttaaaaa aaacacaaaa
     9481 actcagcttt gaagaaataa atacacttca taataaggca gatacaacgg cgattgacgg
     9541 ggaacttgtt cgtctcgcag atcacgcagc ggcatttata gaagcagatc gctcaattaa
     9601 gtttggcata agctctgaac aactaaaaga aggacgagat aatatacgaa acctttatcc
     9661 cgcaggaaaa atccagagcg cttttccaat cggtgatttt tacgcacaat ttctctaaga
     9721 atcaatctca aatctgccga aactaagatg acatgaaaaa atttttacgt ttagcatttt
     9781 taattctctt tttttctctc tctctttctt tttttgccca agcaaaagag cataccgtag
     9841 caaagggaga aaccttatac agcattagcc gttcatacaa cttgactgtt gaagaactct
     9901 gtagcttaaa caatataaag caatccgaag ttctcaaaat cgggcaagtt ttactggtgt
     9961 caaaagccat aaagatacca caggaaacag aaacctatgt ggtgcaagct ggcgacacga
    10021 tgtacagcat tgctcgtaga ctttcagtaa gcgttgaaac actcaatatt ctcaatcaat
    10081 tgagtaacgc ttctcttata aaggtagggc aggttttaac agtaccttct acgacttcta
    10141 ttcagccaga tattagtcaa agtgcgagcg atttaagtct catagaccca cgctcctacg
    10201 ataccaaaaa accagtgaat aagtcgcttc tgtggccagt tactgccctc gatattcatt
    10261 atttatctgg aaaaatttct ggtgtttcta taatgacaaa tgaaaaagaa gcggtaaaag
    10321 cggtgcaaga gggagtggtt atgtacacgg gaccatacag aggctttggt caagttgttt
    10381 ttgtgcaagc gacttctaag catatgtatg tctataccgg tttggaatct ataatcgtgc
    10441 aaaatggaca aagtatccgc aagggcgata gacttggaat agtgggcatc gacgccttga
    10501 gtggaaaaag tgctttgact tttatggttt ttctaaacgg gaatgcaatc gaccctgcga
    10561 aagcaccccg ttaaaaagat ttttaggaaa tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nctgcggatt atattcaatc gattcaccta tatcgcgaat tttttctact
    10741 tttacaccag ctatttcttt taagccatct tttcgaagca tatccatcaa acttttcatt
    10801 tgttgcgcgc cagaagaacc caggaaggtt tttgaaatag caaaatcttc gtggtagccg
    10861 tatgtttcgt aaagttgctc gagtctatcg aggatgctta tgcccttgct tctccagtaa
    10921 agagtcattt ccgcacaaag cgctgcagct gaaatgccgt ctttatcgcg cacttcggtt
    10981 tctatgttat agccatagct ttcttcaaag ccaaagatat agttgtgaga ttttgttttt
    11041 tcaaagtcgc tcataaccga agcgatccac ttaaagccgg ttaaacattc tgcaagatag
    11101 acgtcatttg cctttgcaat gcggtcaacg aagcttgagg ttacaatcga tcgaataatg
    11161 gcgggatttt tcggcatgag tcccagctct tttcgagaga caataatata atctgcgagg
    11221 agaacaccga tttgatttcc cgttatgaga acaaagtctg catcttttcc tggtaccgca
    11281 atgccaaaac ggtctgcatc tgggtctgtt gccattacaa tgtcagcttt ttcttttttt
    11341 gcaagatcga ttgccatttt taatgcagca gcttcttctg gatttggttt ttctaaagtc
    11401 ggaaagtttc catcaggttc tctttgttct ggcacagtga ttacttctaa gccaaggtcg
    11461 cctaagactt tttcaacatg cattgctccc gttccatgaa gcggtgtgta tacaatctta
    11521 acttcttttg ctttttcttt aataagtttt ggtctaaaga gttttgattt taccattgcc
    11581 cagtagggtt catctatttc tgcatcgata atatttagcg agccatttgc tattgcctct
    11641 actcgtgtga tatattttat atcgctaaca gtatttactt cttctataat gcctacgtca
    11701 tgtggctcta ttacttgtga gccatcgttc caatacgcct tataaccgtt gtactcagga
    11761 ggattatggc ttgcagtaac aacgacgcct gtatcacagg caagttcgcg aatagcgaaa
    11821 gaaagctctg gagtagggcg aagacttgaa aataagtatg ttgtaaagcc atttgcggcg
    11881 aagatgaggg cggtagcctc agcaaaaaca tccgaaaagc gccgagagtc ataggcaata
    11941 acggctttta gctctttctt ttttgctttt tcgggaagaa cttttataca atagtttgct
    12001 aaaccttgtg ttactttatg gataacgtga gtgtttatgc gatttgtgcc accaccgata
    12061 attccacgta agccccctgt gccaaactct aaagattgat aaaaccgatc ttcaagttct
    12121 atatagtttt cttctttgag aagtttttct atttcacttt taaaatagct atctctttct
    12181 tcttgtaagt attcgtttgc tttttgaatt atagcttctt tttccatgta atgctcccat
    12241 aaattgaata ttgtagttat tatacaagcc aggctgctta aaatcaagga cagcttttga
    12301 tgagtgcttt gtttttatct aaaaacggaa gaatcaaaga aacaagattt ggatcatatt
    12361 ctctgcctgc tcctttttca agttctttga taagttgttc gcaagcaatt ttttttctat
    12421 agggtaagtc atgagccatt gctacaattg agtcgatgat tcttataatt ctgctttcta
    12481 agggaatagc ttctttaaat aatccatagg gatagccgct tccgtcccag ttttcgtgat
    12541 gatagagaac aaagtttgct aagcccttgt attctggaaa ttccgtgagc agtttttctc
    12601 caatcattgt atgcgatctg acaatgctcc attccatttc actaaggctt tctttttttt
    12661 gaaaaatatg ttcaggtaag cctatttctc caatgtcgta gaggcgactg agttgatgca
    12721 atttttttcg tttataagat gtaaggttga ggaggcgaga aaattcattt actaagactt
    12781 ttttgcattc ttttgaagct tctctatttg aaattttatg ttctattgtt tttaaaaagc
    12841 gttttaagta aaagcgtttt gctcgttcat actgcgtcaa ggtactcaaa tgtaaaaagc
    12901 tatttgcatt tacaaacata gtttccatat ttatgattcc ctctgaatga tgggcgtaac
    12961 cataggaaat aagtacgttt tttgtgtttg tgctattatt atgtttggcc ataaaggttt
    13021 caaaattttt agcaagggct tcggtttcca tttcgcttat atcttctatg atgacggcaa
    13081 aggattcaaa agagatgcgc gctattgtcg tattttccga aaaaaatact gatattattt
    13141 ctccagctaa aacaatgagt tgttcgctgt gtatttgtcc aagtgttgtt tttataaact
    13201 gtaagccggt aacctcaagt ataaaaagtg ccatgtgttt tgctgtaact ttagtaaatt
    13261 catttatttt tgaatcaaac caccttctat tgtagagttt gctaatggga tcaaggttta
    13321 gcaaagaaga atgtattttt tgtaattcta caatgtcaga aatatcacgc acaataatta
    13381 taacttcatt actctgtgtt ttattaaatc gtgcttcaag atgcataagc ttttctttgt
    13441 tttctaaggt aaaagttatc tcatagcttg catgttttag cgcttgttct gctcccattt
    13501 ttaagttttc catagcttct gcatttgcta ggattgggct cataagctta tgtggttcga
    13561 ttcttgtact ggagaagggt tttatctctg ctttttcgtc gtaaataaag agtatgtctc
    13621 gaatcgcatt gataagagtt tcgtttcggt tttgtagctt ctcgagcatt gttatttttt
    13681 ggagtaactc tggataataa ttttttttaa aagattcctc gccaaggccg attatagaat
    13741 ctcttgtata atttttattc aaggattcgc tcatataatt ccttcattgt ttcgatagtc
    13801 attgtttttg gatttgtaac aagacaagga tcttcataag cgttttgagc taactgagac
    13861 agtaagtttt cagattttgt tggtattgtt ttttttgcag gtataccaag agcatttcgt
    13921 agttcctgta tcttttctat gagtattttc tttacttcat catggtttaa attatttact
    13981 tcgagcttca ttttgagagc aatgtttttg tatcgctctg gttcagcatc aaaattataa
    14041 tcaataacat gttcaagaag aatactattg cattcaccgt ggggcagccc caaaagacca
    14101 ccaaggctat gggcgagtgc gtgcacagca cctaagcttg catttgaaaa ggcaaagccg
    14161 gcgtgggtgg atgccatcat cattgtatgc atatcttcga ttgtgcgttc ttttgtaatt
    14221 gctttttcaa tcgtctctga aataagacgg atggcttcga gggcatgagt atcggttata
    14281 gcagagcttg catttgaaac ataggcttca attgcatggg tgagggcatc catacctgtg
    14341 caagcggtaa gataagggtc catagtcata agtggaacag gatctatgag ggctatatct
    14401 ggtatgagtt ttttacttat aattgcaatt tttaaactgc gttttgtatc atttattatt
    14461 gcaaactgag aaacatctgc agaggcaccg gaggttgtag ggatacagca aaggggaggt
    14521 cctggcgtct ttaccatatc gacaccttca tagtcaagta tattgcccgt atttgttgcc
    14581 atgatggcaa tgccctttgc acaatctatg gggcttccac ctcctattgt gacaatgaca
    14641 tcgcaattgt tgttgatata tgctgttaag cctttatgta cttcaatatc ttttggattt
    14701 tccgaaacgt cagagaaaat aataaactca gatacttgtt ttttgattct ttctataatg
    14761 tcagcgaa
//
LOCUS       DAOSSW010000043        14690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_4172_length_14690_cov_7.154561, whole genome shotgun sequence.
ACCESSION   DAOSSW010000043 DAOSSW010000000
VERSION     DAOSSW010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 14690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14690
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4172_length_14690_cov_7.154561"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2996
                     /locus_tag="PKW26_05010"
     CDS             <1..>2996
                     /locus_tag="PKW26_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92957.1"
                     /translation="SWYFEAAFAEGFEKNSIAAGYYGEGFIKHIRLANRGIHFSDLFS
                     SMGLSKDENETPGIYAAFGKDNFQSEAFVRLESSTSHTRQFIGQANINGNLKEDREWL
                     RGQFFTLPSEALTKKILAVYVEDEKGKYVSETFSKTAFRPLTEDEFLVYPAQSLVYLT
                     KKYEGILLFQFNARSDDNEKDAIFSELGFYDDGSSGDTTSFLKKIQNEFCSIITGENL
                     VELGDYAFAGKAEKDENFFVNLENKETGENLENLIMLVQHPSFFSPFENASYYSFITS
                     SPEESFVYTQKTKEPSSLYRSILSTENSLFQFDTAEKNKSFVQVIAQNQEGAQFPFAK
                     TNPNLYLQGKESKTESLYRIEIISSDEGAKASEYLIGSQALLGTIQVFINGIETKNFS
                     YSKNNGKILLEREIRESDKVEISWKEETKGFENAAVKTAVGFNWDFAKLQTSLIFSGD
                     YPIDSKKNFSEIDSIQAAHFESRASLDYKNETSFGLFSLEESLSFSLTNPNVSQILRL
                     DGMNEESKTYYLSKEAVRKIEHTSTIYLNTKDENGSISDATELKPVKFYTADIEINTD
                     EKITGYAWDLSWKDIDTDKWTVVELELDTQDLLSGAAFFSIALKNLSETDEDFDIYLQ
                     LGESLERAGSEIYEKQIPTWKLTGNVQDNFVTKPFDFDSEKDWQTIRVALTNHDRSHL
                     GKNPIARLIIVHTKDEEQTGSLRIGPYEISPDSFKPKNASSQSSDAQSFTFYAPNTGQ
                     KRIQEFNANTINTAQAFFWQGSETYQFKKNLPYLPLQSYKKLAFFYSIPSKNIDLTNN
                     KGLKEFALDFIQEGELSKDAKKVLSIHIDFEELDKEFLSAQWHHLEIDLEEKTLFIDG
                     IKIDKEFYSIDIAENITPNSFEVTILGKENEAGSIYIDELYLSDPYMQSKLRNTFAAN
                     YKTDFSLKLYDFPILEQIQISLKTEQVYSKEKTWLANDDFFSAASELSTHFSLLSFDI
                     KNLLSLHFTNSAEKNLSLDQFSYSIK"
     assembly_gap    2997..3096
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3097..4195
                     /locus_tag="PKW26_05015"
     CDS             <3097..4195
                     /locus_tag="PKW26_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92958.1"
                     /translation="FDLQFSNGEKAKERNEEIHFKTIAGIDYLSLKPELSFKNKFLSL
                     PSENKSRFQPEIQFSLPFKIQDIDFSFQHKKYFRVQEKDFISLNYEEDLEGYFQRLSE
                     AKTFYTSLLFLDLFPSYINANDIWHNRTKGNLENQELSTSYGFQVKRKLYLNPMDIIV
                     PASFDFSLERNIFVLDQIQSKDNFYYLTKLSYTSFNNFGLFGSKKLFSWFEQDELFSS
                     LQCKIKHNLDTNNVAYAIRFFEQVNIYISEDNTLFENLDISFDSEKKLSLYFSVSWHR
                     KGKNSFLKPLMENLMKSENEFLLLRKNSLSYLFEKNTETLNVLRGDHELEIQPNKNLS
                     FITSLNGNIIFTEKKMKSLELDLSLSAKLKF"
     gene            complement(4254..6314)
                     /locus_tag="PKW26_05020"
     CDS             complement(4254..6314)
                     /locus_tag="PKW26_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92959.1"
                     /translation="MKKNVLKKYAVLLLLFFMASGFLFSQDSFSQLREPSLADRGIAE
                     KEFRLGVLSYYRGAYNEAIIQFEKALAFLPQEYLILDWLAKSYYKSGIENAALQTWTL
                     ARDAGYGGMLLENTIEIVKERRSYDKSKSLLQTYSESGVYPGNDERTLFFSNPVSIKP
                     LNDGTSWLVAYGSNEIVRLDINGQILQRFRGPLNGFDRPMDILVLDNGNLVVSEYSGD
                     RLSLLDAKGSYIKSFGSKGRGEGQLLGPQYLAQDSQKNIYVSDFGNARISVFTQDGDF
                     LFYFGLKSKYFSGLEAPTGIAVHEDRVYVCDAVYGSIIIFDTSGNYISHLTEKHVFEN
                     PEALWYWNEHFILADTRSIKAVSLKNGAVETLGNTGKTPGRLTSAAMDVNGNLIASDY
                     ILNEINLLSTMEDLIGGLFVQVERVHAESFPMVRVEVKVENRKGDPIVGLKEVNFLFT
                     ENKRPVSDLTLVGATDNNTVADISLLIDRSLSMKAYEKDIERAVREIASSMDGGRIRI
                     VSLGSSPVTEIQTSPENLRNFSINLLKTPYVNTIALDTGIRLAANDLINAERKRAIVL
                     LSDTERKTGAFRNYGLTELSSYLNTNAIPFCAVSLNQGSLSPEIDYLVSNTKGLASYL
                     YRPEGISSLYKEILSIPKGTYQFTFFSSLPTHFGQSFLPLEVETYLFNKSSRAETGYF
                     APLE"
     gene            complement(6315..6998)
                     /locus_tag="PKW26_05025"
     CDS             complement(6315..6998)
                     /locus_tag="PKW26_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ92960.1"
                     /translation="MYQALKDGISLYKQKKYTEALAFFLTVPDGDEEASLEIAYYLAL
                     CYMQLKKNEEAILYFEQVVTTASDLGRVRQARFLLAVLYSKTGRNRLADFELKKLIES
                     GRQDEKVFSSLAYVSWIQGDSESSIRYYERALELNPEYPSALNGLGYVLASLDKDLAR
                     ALSLCKRAHDYNSSSAAFLDSVAWAYYKLGFLKEAKSFIKKAKRIDSLHPDIIAHYEI
                     IFETKEAAK"
     gene            complement(6983..7726)
                     /gene="surE"
                     /locus_tag="PKW26_05030"
     CDS             complement(6983..7726)
                     /gene="surE"
                     /locus_tag="PKW26_05030"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOQ92961.1"
                     /translation="MKILLTNDDGIMSEGLQVLVSVLEKEHEVWVVAPDRNRSAVSHS
                     ILMDIPLKIKKKAKRQYACSGVPVDCVVHGLRAILKGNPDCIISGINHGANLGTDILF
                     SGTAAAARQGALYGIPSIALSLQSETGTYDFRHLADFVLKHLENILKLSERDVFVNIN
                     AMSPGPYERCVLTKPSRRTYNDGTEHFIAPDGQHYAFFKGGSIDTEDCEKGDYQALVR
                     GDISLSRIHAQPLGLHFDEELWEALCIKP"
     gene            complement(7734..8912)
                     /locus_tag="PKW26_05035"
     CDS             complement(7734..8912)
                     /locus_tag="PKW26_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOQ92962.1"
                     /translation="MEKVVSAHNEEFSSPPDIVLSAPGRFHLVGEHSWYFKDKTLSMA
                     VNLPVYLAISKRPDSSLRFYFPQTKERKRANITSLKYRREDRWANNVKALMFAFVSMG
                     HELPGMNVTVYSDILPSAGFGITTAIKVATALALRTLFSLPYDDKAIMDILEKANRVF
                     LSTEHYVSDLNAALFAKKNTCILTNHKTKMYKTIPFPFKDHTIVLTDARVPRISLWDE
                     ETIRREEYRNVLKDLEIRKNGDWVYEDSEAEIADVLSDVPEDVRRRLICVMKEHQYVV
                     DAVEGLETKDFSKFARAVNKSHEIMRDLYQISCPEIDWLVKRVLEFDSMAIRKADSCA
                     RITGKGFGRCTYTIIKNEDVETYMQKLSDYERIFGFHPVTYIVKPVDGAAIQYPYKKP
                     "
     gene            8993..10741
                     /gene="lnt"
                     /locus_tag="PKW26_05040"
     CDS             8993..10741
                     /gene="lnt"
                     /locus_tag="PKW26_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOQ92963.1"
                     /translation="MSKILQVFYALFSAILLSLAIQNEFLPWGSPFLGLFALVPLYIA
                     FSRAESARESGFLFSLTVSVTHLLSSFWLSNFKDFAVFTIGATTIWYTISCFFLGQLF
                     YGVFFIPPSKKLIIDSGKNSWHIPRRVFLFVSLYILFEWQKSTGFLAYPWGTLYMASY
                     KWQIFSQIAAITGTLGISWLFAFFSSVFAEGLILLNKVPLVKSMHRSFSALAASLFLL
                     FMLSMVYGTIIYTGKKEVIDYFDVILVQQNTDSWESDFQGDKKGILISQALSEKNLGQ
                     MEDKPDLIVWSESNINSLYLPDYYERLEHYPPERPLLPFIRETQVPFAIGGVVRLPQT
                     KNQIKLEQEGFSVEEINFGNAILIFDSDAKLDGYSAKSQLVPFAEAIPYADSLWMQKL
                     MQKTAGFSSGWTAGSEIRLFPLALKSGNTVRIANLVCFEDAFTDLCRKMNNLGADVFV
                     NLTNDSWSKTNSAEIQHFVVASFRALEFRKPLIRSTNSGYTVVVDAYGNRIADLPLFV
                     SDALSVSVPLYKNQLTTYARFGNWVPLCSALFVLFHCLLFLKNKIHAKVKSKENLKGK
                     KDIERTLAKEDSNENS"
     gene            10728..11345
                     /locus_tag="PKW26_05045"
     CDS             10728..11345
                     /locus_tag="PKW26_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOQ92964.1"
                     /translation="MKIANKQNNTKKSLLVLSFTVLIYLSSFFHISPEIFGFYNFAQY
                     PIVLQNMFAVLAGVLLGGTQGAGAVGLYLVLGALGLPLFSKSALIQTQGFERLSGESG
                     GFLVAYFVASLVSGLILKNPKNEKQDIKKIIKATVLGYALIWLLGLLQYLHIKGFQFS
                     LSAIQTVYLVLIQPYIIFDIIKVIITVFLAFFLRPKFSSFFPENY"
     gene            11454..11921
                     /gene="nrdR"
                     /locus_tag="PKW26_05050"
     CDS             11454..11921
                     /gene="nrdR"
                     /locus_tag="PKW26_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOQ92965.1"
                     /translation="MRCPYCGSFDDKVLESRTMAGGESIRRRRECLSCSYRFTSYEHI
                     EEKPFMVIKRDNRRQPFDRHKLEKGIERALEKRPISTATKESIINEIEDHAVMHGRTT
                     REISTNELGELVLEKLYEVDKVAYIRFASVYKHFESLEEFIEEVNKLEGKNGQ"
     gene            11911..14013
                     /locus_tag="PKW26_05055"
     CDS             11911..14013
                     /locus_tag="PKW26_05055"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOQ92966.1"
                     /translation="MGNETVFPEWKSFLGKANDAETAMFIKHVVKRSGEIGDYDRLKI
                     KTAIGKAIEAVEKRPNPDKAELLTEAVEEKIRLMMAGRHAQSIPAIEEIQDIVEEVLI
                     EHSEVGIAKAYILYRAKREAIRDTKKLMVDINTTMDGYLSQSDWRVNENANVNFSLGG
                     LILHNSGTITANYWLKNIYSPEVAEAHQTAAFHIHDLSMFSGYCAGWSIRQLIAEGLG
                     GVPDKITSKPATHLSTLVNQIVNFLGIMQNEWAGAQAFSSFDTYLAPFVRVDNLSERE
                     VRQCIQSFIYGVNTPSRWGSQAPFTNITLDWNVPEDLRNTPAVVGGKDQDFTYGDCQA
                     EMDVINKVFIELMIEGDAAGRGFAYPIPTYNITKDFDWDSDNARLLFEMTAQYGTPYF
                     QNFVNSDLNPSDVRSMCCRLQLDKRELRKRGGGLFGSDEFTGSLGVVTINLPQIAFLS
                     KNENEFFSRLNYLMDLAKESLSAKRKVIQRLLDGGLFPYTRRYLSTLDNHFNTIGLCG
                     MNEACLNFLGRDIVHPLGKDFAEKVLLHMRKRLADYQETTGELFNLEATPAESTSYRL
                     AKHDVKNFPGIITSGDDEPFYTNSSQLPVDYTEDVFDALDHQEALQTKYTGGTVFHTF
                     MGEAIKDWKACRDLVRAVTGNYKVPYITISPTYSICSCHGYLEGEHFECPKCKAEAEQ
                     NLRMQIAALEIEKEKLLA"
     gene            14114..14668
                     /gene="nrdD"
                     /locus_tag="PKW26_05060"
     CDS             14114..14668
                     /gene="nrdD"
                     /locus_tag="PKW26_05060"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOQ92967.1"
                     /translation="MATVTKRNVQTIEAEIAQKKEALQNVRGRETEVYARIVGYYRSV
                     RNWNKGKREEYDHRKMFEYENNSEESSFSQIDVQSEADFKSYEAEKKSSHTSADSDIR
                     YEFFSRKTCPNCPAVQEYLETLSFSGKYINVDSAEGLERARELQILSAPTVVIFNEAG
                     DEVARAQSVKELTEILEPALTAAS"
BASE COUNT         4694 a         2978 c         2681 g         4237 t
ORIGIN      
        1 tcctggtatt ttgaagcagc ctttgcagag ggctttgaaa aaaacagcat tgcagcaggt
       61 tactatggcg agggttttat aaaacatatt cgtctcgcaa atcgagggat tcattttagc
      121 gatttgtttt caagcatggg gctttcaaag gatgagaatg aaacacccgg tatctacgca
      181 gcttttggaa aagacaattt tcaaagtgaa gccttcgttc gcttagaatc gagcacaagt
      241 catacaagac aatttatagg acaggcaaat ataaacggca atctaaaaga ggatagagaa
      301 tggcttaggg gacagttttt caccctcccc tctgaagcct taacaaaaaa aatcctcgct
      361 gtctatgtcg aagatgaaaa aggaaaatat gtttctgaaa ctttttctaa aactgcattt
      421 cgccccttaa ctgaagatga gtttctcgta taccccgccc aatccctcgt ctatttgaca
      481 aaaaaatatg agggaatact tctctttcag tttaatgcaa gaagtgatga taacgaaaaa
      541 gacgccatct tttctgaact tggtttctat gatgatggtt caagtggaga tactacaagc
      601 tttttaaaaa aaatacaaaa cgaattttgc tcgattataa caggagaaaa tcttgtagag
      661 cttggagatt acgccttcgc aggaaaagca gagaaagatg aaaacttttt tgttaactta
      721 gaaaataagg aaacaggaga aaatctagaa aacctcatca tgctcgttca acatccctct
      781 tttttctcac cctttgaaaa tgcctcctac tatagcttta taacttccag cccagaagaa
      841 agctttgtgt atacgcaaaa aactaaggag ccgtcttctc tctatcgctc catcctatcg
      901 acagagaact ctctttttca atttgataca gcagaaaaaa ataaaagctt tgtgcaagtt
      961 atagcgcaaa atcaagaagg cgctcaattt ccttttgcca aaacaaatcc aaacctctat
     1021 ctccagggaa aagaaagcaa aacggaatcc ctttatagaa tagaaatcat aagctcagat
     1081 gaaggcgcaa aggcttcaga atatctcatt ggatcccaag cgctactcgg tacaatccaa
     1141 gtttttatca atggaataga aacaaagaac tttagctata gcaaaaataa tgggaagatt
     1201 ctccttgagc gagaaatcag agaaagcgat aaagttgaaa taagctggaa agaagagaca
     1261 aagggttttg aaaacgcagc tgtaaaaact gcagtgggct ttaactggga cttcgcaaaa
     1321 ttacaaacaa gccttatctt tagcggcgac tatcccatag attcaaaaaa aaactttagt
     1381 gagattgatt ctatacaagc agctcacttt gaaagcaggg caagtctcga ttataaaaac
     1441 gaaacaagct ttggtttatt ttctctagaa gaaagccttt ccttttctct gacaaatccc
     1501 aatgtttcac aaatactacg gcttgatggt atgaacgaag agagcaaaac ttactatctt
     1561 tcaaaagagg ctgtaagaaa aattgaacac acaagtacca tttatttaaa tacaaaagat
     1621 gaaaatggct caataagcga tgccactgaa cttaaacctg taaaatttta taccgcagat
     1681 atagaaatca atactgacga aaaaatcacc ggctatgctt gggacctttc ttggaaagat
     1741 attgatacag ataaatggac ggtggtagaa ctcgaactag atacacagga cctcctctct
     1801 ggtgctgcat ttttttctat agcgctgaaa aatctaagtg aaactgacga agattttgat
     1861 atttatcttc aactcggtga atccttagaa agggctggtt cagaaatcta tgaaaaacaa
     1921 attcccacat ggaagctcac tggtaacgtt caagacaact ttgttacaaa gccttttgat
     1981 ttcgattcag aaaaagactg gcaaacaata cgagtagccc tgacaaatca cgatagaagc
     2041 catcttggta aaaatcctat tgcgcgcctt attattgtgc atacaaaaga cgaagaacaa
     2101 acgggctctt tgagaattgg tccctatgaa ataagcccag atagttttaa gcctaaaaat
     2161 gccagctctc aatcaagtga tgcccaaagt tttaccttct atgcgccaaa tacgggacaa
     2221 aaacgcattc aagaatttaa tgccaacaca ataaatacag cacaggcatt tttttggcaa
     2281 ggtagtgaaa cgtaccaatt taaaaaaaat cttccctacc tcccattaca gtcatataaa
     2341 aagctcgctt ttttttattc tatacctagc aaaaacatag atttaactaa taacaaaggg
     2401 ttaaaagaat ttgctctaga ttttatccaa gagggagagc tttcaaaaga tgcaaaaaaa
     2461 gttttatcta tacatataga ttttgaagaa cttgacaagg aatttctttc agcccaatgg
     2521 catcatcttg aaattgactt agaagaaaag acgcttttta tagacggcat aaaaatagac
     2581 aaagaattct atagcataga catagcagaa aacatcacgc ctaactcttt tgaagtgact
     2641 attcttggaa aagagaatga ggcaggaagc atatatattg atgagcttta tctctcggat
     2701 ccctatatgc aatcaaagct cagaaatact tttgcagcaa actataaaac agatttttca
     2761 ttgaagctat acgacttccc catcttagag cagattcaaa taagcctaaa aacggagcaa
     2821 gtctatagca aagaaaaaac ctggcttgca aatgatgatt ttttttctgc tgcatcggaa
     2881 ctgagcacgc attttagtct tttaagcttt gacataaaaa atcttcttag tctacatttt
     2941 accaactccg cagaaaaaaa tctttcacta gatcagtttt cctatagcat caaaagnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncttc gacttacagt tttcaaatgg
     3121 agaaaaggct aaagaacgaa acgaagaaat acattttaaa acaattgcag gcattgatta
     3181 tctttcactc aaaccagaac tcagttttaa gaacaaattt ctgtctcttc cctctgaaaa
     3241 taaatctaga tttcaacctg aaatacaatt tagtcttccc tttaaaatac aggatataga
     3301 tttttccttc caacacaaaa aatattttcg ggtacaagaa aaagatttta tcagtctcaa
     3361 ttatgaggaa gaccttgaag gatattttca aagactgagt gaggcaaaaa ccttttacac
     3421 gagcctttta tttttagatt tatttccaag ctatatcaat gcaaatgata tatggcataa
     3481 tcgaacgaag ggaaatcttg aaaaccaaga actctcaact agctatggct ttcaagtaaa
     3541 acgaaaactc tacctcaatc caatggatat tattgtccca gcaagttttg atttttcact
     3601 tgaacgaaat atttttgttc ttgatcaaat ccaaagcaag gacaattttt attatcttac
     3661 aaaattatca tatacctctt ttaataactt tggacttttc ggatcaaaaa aacttttttc
     3721 atggtttgaa caagatgaat tatttagctc tctacaatgc aaaataaaac ataacctaga
     3781 caccaataat gtagcttatg cgatacgttt ttttgaacaa gtaaatatat atatttcaga
     3841 agacaatact ctttttgaaa accttgatat atcttttgac agcgaaaaaa aactctcact
     3901 ttatttttca gtctcttggc atagaaaggg aaaaaattct tttttaaagc cattgatgga
     3961 aaacctcatg aagtctgaaa atgagtttct ccttctgaga aaaaacagcc tcagttatct
     4021 atttgaaaaa aacacagaga ctttaaatgt tcttcgtgga gatcacgaat tagagataca
     4081 gccaaataaa aacctcagct tcataacaag tctgaatggg aatattattt ttactgagaa
     4141 aaaaatgaag agccttgagc tcgatctaag cctatctgca aaacttaaat tttaattttt
     4201 tatatctctt ccctgaaaaa tagttcagaa aattatctat gtccaaaaac gttttattca
     4261 agaggagcaa aataacctgt ttccgcccta ctagatttat tgaacaaata agtttcaact
     4321 tctagcggca aaaaactttg tccgaaatga gtaggaaggc tagaaaaaaa ggtgaactgg
     4381 tatgtacctt tgggaataga aagtatttct ttatacaatg aagaaatgcc ctcaggtcga
     4441 tacaagtagg aagcaagacc cttcgtattt gaaacaaggt aatctatttc tggagaaaga
     4501 gacccttgat tcaaagagac agcacaaaaa ggaatagcat ttgtatttaa gtaagaactt
     4561 aactcagtta aaccataatt tctaaaggct cctgtctttc tttctgtatc ggataaaagt
     4621 acaatagctc tttttctttc agcatttatg agatcatttg cagcaagccg aatgccggta
     4681 tcaagggcaa tagtatttac atagggtgtc tttaaaagat ttattgagaa atttcttaaa
     4741 ttttctggac tcgtttgtat ttcagttacg ggagaacttc cgagcgatac aatgcgtatt
     4801 ctaccaccgt ccatcgaaga agcaatttcg cgaacagccc tttctatatc tttttcgtat
     4861 gccttcatag aaagagagcg gtctatgagc aagcttatat ctgccacggt attattgtct
     4921 gtagcaccga caagagtcaa atctgaaact ggtcgtttat tttcggtaaa taaaaagttc
     4981 acttccttta agccgacaat tggatcccct ttcctgttct caacctttac ttcaaccctt
     5041 accataggga aagattcagc atgcactctt tcaacttgga caaataaacc accgattaaa
     5101 tcttccatag tcgataaaag atttatctca tttaaaatat agtcgcttgc aattagattt
     5161 ccatttacat ccatcgccgc gctggtaagc cgacctggcg tcttaccagt atttcccaag
     5221 gtttctacag caccattttt caaagatacc gcttttatgc tgcgtgtatc tgcaagaata
     5281 aaatgttcat tccaatacca cagcgcctca ggattttcaa aaacgtgttt ttctgttaaa
     5341 tgagaaatat aattccccga cgtgtcaaag atgataatgc taccgtaaac agcatcgcaa
     5401 acatagaccc tatcttcatg aacagctatc cctgtcggtg cttctaaacc cgaaaaatat
     5461 tttgacttta agccgaagta aaataaaaaa tcaccatctt gggtaaaaac agaaatacgc
     5521 gcatttccaa aatcgctgac ataaatattt ttttggctat cttgcgcaag gtattgtggt
     5581 cctaagagtt gcccctcgcc tcttcccttt gagccaaaac tttttatata tgaacccttc
     5641 gcatcaagca agcttaaacg gtcaccgctg tattcactga caaccaagtt tccattgtcc
     5701 aaaactaaga tatccatggg gcgatcaaag ccgttaagcg ggccccgaaa acgctgcagg
     5761 atttgcccgt taatatcgag gcggacaatt tcatttgagc cataggcaac aagccagctt
     5821 gtgccatcgt tcaagggctt tattgaaaca ggattgctaa aaaaaagagt acgctcatca
     5881 ttcccaggat agaccccaga ttcgctataa gtttgcaata gcgatttaga tttatcatag
     5941 gagcgtcgct ctttcactat ttcaattgta ttttccaaaa gcatgccacc atagcccgcg
     6001 tcacgagcaa gcgtccacgt ttgtaaggcg gcgttttcta taccactttt ataataagac
     6061 ttcgcaagcc aatccaagat gagatactct tgcggcagaa aggcgagcgc tttttcaaac
     6121 tgaataatcg cttcattgta tgcccctcga taatatgata aaacaccgag gcgaaattct
     6181 ttttcagcaa tgccgcgatc tgcaaggctt ggttctctta attggctaaa gctatcttga
     6241 gaaaaaagaa agccactcgc cataaaaaaa agaagaagaa ggacagcgta tttttttaat
     6301 acattctttt tcatctattt cgcagcctcc tttgtttcaa aaatgatttc ataatgagct
     6361 ataatatcgg gatgtaagga atcaatgcgt ttcgcttttt ttataaagct ttttgcttct
     6421 tttaaaaaac caagcttata ataggcccaa gcaacagaat ccaaaaaggc tgccgatgag
     6481 ctattataat cgtgggctcg tttacaaagc gaaagtgccc gtgccaaatc tttatccaaa
     6541 gatgcaagca catagccgag accattcaat gccgaaggat actcgggatt tagctccaaa
     6601 gcgcgttcat aatagcgtat agaagattcg gaatctcctt gaatccaaga gacataggca
     6661 agagaagaaa acactttctc atcttgtcta ccactttcta taagtttttt tagttcaaaa
     6721 tctgcaagcc gattccgtcc tgtttttgag tacaaaactg caagtaaaaa tcgagcctgt
     6781 ctcacacgcc ccagatcact tgccgttgtt acaacttgtt caaaatataa aatagcttct
     6841 tcgttttttt tcaactgcat ataacaaagg gcgagataat aggctatttc gagtgaagct
     6901 tcttcatcac catcagggac ggttaaaaaa aaggcaagcg cctctgtata ttttttttgt
     6961 ttgtatagag aaattccgtc ctttagggct tgatacatag ggcctcccag agctcttcgt
     7021 caaaatgcaa acccaatggc tgtgcatgaa tacgagaaag cgagatatcg ccgcgaacaa
     7081 gcgcttgata gtctcccttt tcacaatctt cagtatcgat ggaaccgcct ttgaaaaaag
     7141 catagtgttg cccatcaggt gcaataaaat gttccgtccc gtcattatag gttcgccttg
     7201 aaggcttcgt taaaacacaa cgctcatagg gtcccggaga catagcattg atatttacaa
     7261 aaacgtccct ttcagacaac tttagaatat tttcaagatg ctttaaaaca aagtctgcta
     7321 aatgccgaaa gtcataggtc cctgtctcac tttgtaagct taatgcaatg ctcggtatgc
     7381 catacaaggc accctgtcgt gcagctgctg cagtgccaga aaaaagaatg tcagtgccaa
     7441 gatttgcgcc gtggtttatc cctgaaataa tgcagtcagg atttcccttt aaaatagctc
     7501 gaagaccatg gacaacacag tcaacaggca caccagaaca ggcatactgt cgtttcgctt
     7561 ttttcttaat ttttagcgga atatccatca atatgctatg tgaaacagcg gatctatttc
     7621 gatcgggcgc aacaacccaa acctcgtgct ctttttccaa aactgaaact aaaacctgca
     7681 agccttcact cattataccg tcatcatttg taagtaaaat tttcatacaa agctcaaggt
     7741 tttttatacg gatattgtat cgccgcgccg tcaactggct ttacaatata ggttactgga
     7801 tgaaagccaa agattctctc ataatcagaa agtttttgca tataggtttc aacatcctcg
     7861 ttttttatta ttgtataggt gcaacgtcca aagcctttac ctgttatgcg ggcacaagaa
     7921 tcagccttac gaattgccat agagtcaaac tctaggacac gcttcacaag ccagtctatc
     7981 tctggacatg atatttgata taggtcccgc attatttcat gggacttatt taccgcacga
     8041 gcaaattttg aaaaatcttt tgtttctaag ccctctacag catcgacgac gtattgatgc
     8101 tctttcatca cacaaatgag acgacgacga acatcttcag gaacatcgct caaaacatcg
     8161 gctatttctg cctctgaatc ttcatacacc cagtcgccat tttttcttat ttctaagtcc
     8221 tttagtacgt ttctatactc ttctctgcga atcgtttctt cgtcccacaa tgaaatccgt
     8281 ggaacccgag catcggtcaa tacaattgta tgatctttaa aaggaaaggg aattgtttta
     8341 tacatttttg ttttgtggtt tgtcaaaata caggtgtttt tcttagcaaa taaagctgca
     8401 ttcaagtctg aaacataatg ctcagtactt aaaaaaacgc gatttgcttt ttctaaaata
     8461 tccattatcg ccttatcatc gtagggtaaa gaaaataatg tacgcagggc aagggctgtt
     8521 gcaaccttaa tcgctgttgt aatgccaaag ccagctgatg ggagaatatc ggagtacacc
     8581 gtaacattca tacctggtag ctcatgaccc atagaaacaa aggcaaacat aagcgccttt
     8641 acgttattgg cccaacgatc ttctcgtcta tattttaaac tcgttatatt cgctcgtttt
     8701 cgttcctttg tttgtggaaa ataaaaacgc aaggaactgt cggggcgttt tgaaatagcg
     8761 agatagacag gcaagttaac agccatggac aaggttttat ctttaaaata ccatgaatgc
     8821 tctcctacga ggtgaaagcg tcctggtgct gacaagacga tgtccggggg agaactaaat
     8881 tcttcattgt gcgcagaaac tactttttcc attcatttaa cctattttac tattcacttt
     8941 taaattccaa gatattgttt tctctaacgt tatcatatag aatgataata taatgagcaa
     9001 aatattacaa gttttttatg cacttttttc tgcaattcta ctttccttag cgattcaaaa
     9061 cgaatttctt ccctggggct cacctttttt gggacttttt gccctcgtac cgctttatat
     9121 tgctttttct cgtgcagaat cagcaaggga aagtggtttt cttttttctc tcaccgtttc
     9181 agtaacgcat cttttatcga gtttttggct cagtaatttc aaggattttg cagtttttac
     9241 tattggcgct accacaatct ggtatactat ctcctgtttt tttctcggtc agctgtttta
     9301 cggtgttttt tttattcccc cttcaaaaaa actcattatt gactccggga aaaattcctg
     9361 gcatatacca cggagagttt ttttatttgt atccctatat atcctctttg agtggcaaaa
     9421 atcaactggt tttttagcct atccttgggg aacgctctat atggcttcat acaaatggca
     9481 aatctttagc caaattgcgg ccatcacggg gactctcggt atttcatggc tctttgcctt
     9541 tttttcatcc gtctttgccg aaggactcat ccttctcaac aaagttcccc tcgtcaagtc
     9601 aatgcatcgc tctttttccg ccctcgctgc aagtttattt ctcctcttta tgctaagcat
     9661 ggtgtacgga acgattatct atacaggaaa aaaagaagtt atagactatt ttgatgtcat
     9721 tcttgtccaa caaaataccg acagctggga aagtgatttt caaggcgata aaaaaggcat
     9781 tcttatttcg caagcactga gcgaaaaaaa ccttggacaa atggaagata agcccgacct
     9841 catcgtatgg agcgaaagca atataaactc tctttatcta cccgattatt atgaacggct
     9901 tgaacactat ccaccagagc gtcccctgct tccctttatt cgcgaaacgc aggttccctt
     9961 tgcgataggt ggcgtcgtac gcttaccaca aacaaaaaat caaataaaac tagaacaaga
    10021 aggcttttct gtagaagaga ttaactttgg aaatgctatt ctcatattcg atagcgatgc
    10081 aaagcttgac ggatactcgg caaaatcaca gctcgtcccc tttgctgaag ctattccgta
    10141 tgctgacagc ctatggatgc aaaaactcat gcaaaaaaca gcaggttttt cgagtggctg
    10201 gacagccggc agtgaaatac gtctctttcc tctcgcctta aaatctggca atacggtacg
    10261 tatcgcaaat ctcgtttgct ttgaagatgc ctttaccgac ctttgccgaa aaatgaacaa
    10321 tttgggagca gatgtttttg taaacctcac aaacgattct tggtcaaaga caaactctgc
    10381 tgaaatacag cactttgttg ttgcatcctt ccgtgcactt gagtttcgaa aaccgcttat
    10441 acgaagtaca aactcgggct acacggtagt cgtcgatgcc tacggaaatc ggatagctga
    10501 tttacccctt tttgtctccg atgccctcag tgtttcagta ccgctctaca aaaaccaact
    10561 aacaacctat gcacggtttg gaaattgggt ccctctttgt tcggccctct ttgttctctt
    10621 tcactgcctc ctttttctca aaaataaaat ccatgccaag gtaaaatcga aggaaaattt
    10681 gaagggaaaa aaagatatag aaagaacact agccaaggaa gatagcaatg aaaatagcta
    10741 acaagcaaaa caatactaaa aaatcgcttt tagttctctc tttcactgtt ttgatctacc
    10801 tctcttcatt ttttcatatt tcccccgaga tctttggctt ttataatttt gcacaatacc
    10861 ccatcgtttt acaaaatatg tttgctgttt tagcaggcgt ccttctcggt ggaacgcaag
    10921 gagctggagc tgtcggactc tacctcgttc tcggagcact cggactccct cttttttcaa
    10981 agtcagcttt aatacaaaca caaggtttcg agcgactttc gggagaaagc ggcgggtttt
    11041 tagttgcata ttttgttgca agccttgttt ctgggcttat tctcaaaaat cctaaaaacg
    11101 aaaaacaaga tataaaaaaa attataaaag caacagtcct tgggtacgct cttatttggc
    11161 ttttaggact cttgcaatac cttcatataa aaggctttca attcagtcta tctgcaatac
    11221 aaacagttta cctcgtttta atccaaccct atattatttt cgatattata aaagtaatta
    11281 tcactgtttt tttagccttt ttcttacgtc caaaattttc ttcttttttt ccagaaaatt
    11341 attaaaaaaa aacaaagttt tatctctctt ttttatcttt ttcacttgca tttatgtata
    11401 tagtgttata tcatctattt agaagtaaaa aaacactaga tacaggatga attatgcgat
    11461 gtccctattg tggcagtttt gacgataagg tacttgaatc gagaactatg gctggaggcg
    11521 agagcattcg ccgaagacgt gaatgtcttt cttgcagtta tcgatttacg agctatgaac
    11581 acatagaaga aaaacccttt atggttataa aacgcgataa tcggcgacag ccctttgaca
    11641 ggcataaact tgaaaaaggc attgagcgcg cattggaaaa acgacctata tctacggcaa
    11701 caaaagaaag cattataaat gaaatagaag atcatgcggt aatgcacgga aggacaacaa
    11761 gagaaatttc tacaaatgaa ctcggggagc ttgttttaga aaagctctat gaagtagaca
    11821 aggttgctta tattcgcttt gcatcggtat ataagcattt tgaaagctta gaagaattca
    11881 tagaggaagt aaataaactg gagggcaaga atgggcaatg aaacggtatt cccagaatgg
    11941 aaaagttttt tgggaaaagc gaatgatgct gaaacggcca tgttcatcaa acacgtagtg
    12001 aaacgatcgg gtgaaatagg ggattatgat cgtttgaaaa tcaaaacagc catcgggaag
    12061 gcgattgaag cggtggaaaa aagaccaaac ccagataagg cagaattgct taccgaagcg
    12121 gtagaagaaa aaattcgcct tatgatggct ggtagacatg cacagtcaat tcctgcgatt
    12181 gaagaaattc aggatattgt agaagaagta ctcattgagc attccgaggt ggggattgcc
    12241 aaggcttata ttctctaccg tgcaaagcgc gaagcaattc gtgatacaaa aaaactcatg
    12301 gtcgatatca atacaaccat ggacggctac ctaagccaat cggactggcg agtaaacgaa
    12361 aatgcaaacg tcaatttttc tctcggcggt ctcatccttc acaactctgg gacaattaca
    12421 gctaattatt ggcttaaaaa tatttattca ccggaagtgg ccgaggcgca tcaaacggca
    12481 gccttccata tccatgattt atcgatgttt tcaggttatt gtgccggttg gtctattcgt
    12541 caactcattg cagaaggctt aggcggagta cctgataaaa tcacctcaaa gcctgcaacc
    12601 cacctttcaa ccttggtaaa tcaaattgta aactttctcg gtatcatgca aaatgaatgg
    12661 gctggagcac aggcttttag ctccttcgac acctatcttg caccctttgt gcgagttgac
    12721 aatcttagtg agcgagaagt acgccaatgt atccaaagtt ttatctacgg agtaaataca
    12781 ccaagtcgtt ggggttcaca agcgcctttt acaaatatta cccttgactg gaatgtgcca
    12841 gaagatttac gaaacactcc tgcggttgtc ggtgggaaag accaagactt cacctacggc
    12901 gattgtcaag cagagatgga cgtaataaac aaggttttta ttgagctcat gattgaggga
    12961 gatgctgccg gtcgtggctt tgcctacccc attccaacct acaatattac taaagacttt
    13021 gactgggata gcgacaatgc acggctttta tttgaaatga ccgcccagta cggcacaccc
    13081 tattttcaaa actttgtaaa ctctgactta aaccccagtg atgtacgttc tatgtgctgt
    13141 cgtttacaac tcgataaaag agaattgcga aaacgcggtg gtggactttt tggctccgat
    13201 gagtttactg gctccttagg tgttgtaaca atcaacctgc cacaaatagc ctttctttca
    13261 aaaaatgaaa atgaattttt cagtcgctta aactacctca tggaccttgc aaaagaaagt
    13321 ctcagtgcaa aacgaaaggt tatacaaaga cttctagacg gagggctttt cccctacaca
    13381 agacgctatc tttcaacctt ggacaatcac tttaatacaa tcggactttg cggtatgaac
    13441 gaagcctgcc taaacttttt gggtcgcgat atagttcatc cacttggaaa agattttgct
    13501 gaaaaagtgc tgctccatat gagaaagaga ctcgctgatt atcaagaaac tacgggagaa
    13561 ttatttaacc ttgaagcaac tcctgcagaa agtacctcct atcgcttggc aaagcacgat
    13621 gtaaaaaact tccctgggat tattacttca ggtgatgacg aaccctttta cacaaactct
    13681 tcgcaactgc cagttgacta taccgaagat gtgtttgatg cccttgatca tcaagaagct
    13741 ttgcaaacca agtataccgg gggcaccgtt tttcacacct ttatgggtga ggcaattaaa
    13801 gactggaagg cttgtagaga cctcgtacgt gcggttaccg gaaactacaa ggttccctat
    13861 attactattt ctccaaccta ctctatttgc agttgtcatg gctacttaga aggcgaacat
    13921 tttgaatgcc caaaatgtaa ggccgaagct gaacaaaact tacgcatgca gattgctgca
    13981 cttgagatag aaaaggagaa gcttttagca taaaaatagc actataatat ttataggtga
    14041 gtctattaca tacaagggtg tctcgttgca gtacacttac gattacaact atagttattc
    14101 tgggaggaaa aaaatggcta cagtaacaaa aagaaacgta caaacgatag aagcagaaat
    14161 tgctcaaaaa aaggaagcct tgcaaaatgt gcgaggcagg gaaacagaag tgtatgctcg
    14221 aattgtcggc tactatcgat ctgttcgcaa ttggaataag ggcaagcgtg aagaatacga
    14281 ccatagaaaa atgttcgaat acgaaaacaa ttctgaagaa tcaagttttt cacagattga
    14341 cgtacaaagc gaagctgatt ttaaaagcta tgaggcagaa aaaaagagca gtcatactag
    14401 cgcagattct gacatacgct acgagttttt ttcacgaaaa acctgtccaa actgcccagc
    14461 tgtgcaagaa tacctcgaga cactttcttt ttcaggaaaa tatatcaatg tagatagcgc
    14521 tgaagggctt gaaagagcac gtgagttaca aattctctca gcaccaaccg ttgttatttt
    14581 caacgaagca ggagacgaag tagcacgagc acagtcggta aaagaattga cagagattct
    14641 tgagccagct cttactgcag cttcttagtc tgtatactaa aaaaacattg
//
LOCUS       DAOSSW010000044        14668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_4181_length_14668_cov_6.171970, whole genome shotgun sequence.
ACCESSION   DAOSSW010000044 DAOSSW010000000
VERSION     DAOSSW010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 14668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14668
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4181_length_14668_cov_6.171970"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PKW26_05065"
     CDS             <1..225
                     /locus_tag="PKW26_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92968.1"
                     /translation="FLISGITRQDWVVFEGNYFYAIPEENTKVLRVVEKPVAVRLLLE
                     TNDWAHIAYPILKTKSEVQHAWIKKTEIRK"
     gene            252..812
                     /locus_tag="PKW26_05070"
     CDS             252..812
                     /locus_tag="PKW26_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOQ92969.1"
                     /translation="MEDLYAILGVSKKASADEIKKQYRKLAQKYHPDLNPGDRAAEER
                     FKSISAAYSILGDEAKRKDYDFQLENPFQARNYANQATSSQDPFWEWFNQEAFSQKNR
                     GYTQYTWSTNNSKSDKNEQVPLTRAEATSLFLRGLFSVLLGLLFFRISFILIPFGPLL
                     CILGIAKGVSNAARGIKDFFLSFKVK"
     gene            complement(868..1119)
                     /gene="infA"
                     /locus_tag="PKW26_05075"
     CDS             complement(868..1119)
                     /gene="infA"
                     /locus_tag="PKW26_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOQ92970.1"
                     /translation="MLYLYTIYGGFVSKEEAIEVEGIVKEALPNTMFRVELQNKHVIL
                     AHLSGKIRKHYIRIVPGDKVKVALSPYDLNRGRIIFREK"
     gene            1280..1636
                     /locus_tag="PKW26_05080"
     CDS             1280..1636
                     /locus_tag="PKW26_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HOQ92971.1"
                     /translation="MEKNFIEKMKETLLAQKQELINNLIANNSDFQNIVDGLTGKDAV
                     DVATDDVDRKMLEALGAKDVNKLKLIDSALSRIEQNKYGLCIKCGKKIPQERLEAIPS
                     ALLCIECKTEDERKNR"
     gene            complement(1633..2517)
                     /locus_tag="PKW26_05085"
     CDS             complement(1633..2517)
                     /locus_tag="PKW26_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOQ92972.1"
                     /translation="MALKQIKTNYHTHSTWCDGRYSTKEMILAAIEKGFTHLGFSSHA
                     MYPFSTKYHLNPKTIQDYTNEIQSLKEDFKDKIEIFHGFETEFIPPLSKPSYALYKDF
                     SPDYLIGSVHYLFNPDARAIKNKAGIPDFFFPVDNTAEELVSGIQRVFNGNTKKAIQT
                     YFYLQREMMKQGDFDIIGHIDLIKKRNQEIAFYDENARWYKNELLATAKEAKRQNKIV
                     EINTSALARSLHKEVYPSTVFIDYLVSLGVPLMLNSDSHSPRFLDSEFENTKELLKTR
                     GLRELFYLTKKGWERQSL"
     gene            complement(2508..4505)
                     /locus_tag="PKW26_05090"
     CDS             complement(2508..4505)
                     /locus_tag="PKW26_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ92973.1"
                     /translation="MSLRQAFNPLSSRFFTTTVHELRTPIQSIIGTTELLQQTELDKE
                     QAEYLRQLRFSSDVLLNLVNDVLDLSKLISGQVKTEHIFFSLSDLCEQVVDLVCIEAH
                     NKKIEIITDIDYRLPPSIKGDPHRTQQILLNLLKNAVKFTKEGYVHLSVFLDTSKTNI
                     IFEVSDSGIGIPKEKQENLFEDFYQVDNSTTRKFGGTGLGLSISKGLTKLLGGKIGMK
                     DNPSGGSLFWFSLPCIFQNATKPIKPAIPLDPQTRILLVDDNVLSLESFTKKLQFLGF
                     EKIHVATSGQTALDMLKEAAEKKKPYKIAFIDMIMPVMDGWRLSAEINSDENINTTML
                     FLMVPEGQMGGEAKMKMLDWFNGYLYKPLKKKMLIETLKSISSDSSLDLEPIEIEKPK
                     ATGEKILFKQVLIAEDHPINQKLLKTFLEKLEIEVFTASNGKEAILCLNKNRGISFIF
                     MDIQMPLMSGVEATRFLRKKKYKGIIVACTANTNEEDFTTYKQVGMNDYLLKPFKQND
                     VAKLLEKWSSCFIPTEKYPRQISKTKKILQTSWDSADLLDTVAGDVFLGKQLITQYIQ
                     QTRSALINARESLFQKNFERLASIAHALKGSSNAIAAHALALQAEDLEEYINTMLSEK
                     TQAIEKKHDINTEVITSGLNNFSALFLQFEKQSQEVIDSWL"
     gene            complement(4507..>4929)
                     /locus_tag="PKW26_05095"
     CDS             complement(4507..>4929)
                     /locus_tag="PKW26_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ92974.1"
                     /translation="MKFYSDSGVLDSSDKKINDNPHIAIVDDDQVTRTILQNTFKQIN
                     AEISLFDSGSEFLMATNKHIFDLIILDIYMPGLSGFDILKTLHSKQYKTPIIIYSNAT
                     SKESVVQALSLGAKSYLVKPLKPEALLQKAIEVLHSRI"
     assembly_gap    4930..5029
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5030..5533)
                     /locus_tag="PKW26_05100"
     CDS             complement(<5030..5533)
                     /locus_tag="PKW26_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ92975.1"
                     /translation="MKQVLIIDAPPLFREFLKEKLSHEKVNVEIAHIKRDAFTKLVSS
                     LPDLLIIDLSVSIREIIDFLAKKNEDPNASRIPVFMTGPIITQDQIALLSQFNVVKYF
                     NKPIKFDVFFESIGKVLKTGFSIDTTPCILELHLNNDIIFIEIAQGLNREKISLLKYR
                     ISEIIEQN"
     gene            5601..6221
                     /locus_tag="PKW26_05105"
     CDS             5601..6221
                     /locus_tag="PKW26_05105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOQ92976.1"
                     /translation="MNKKDVHIVILLADGFEEIETVVPYDLLVRAGFTVKTIAFSEDS
                     LVSGSRGLKIQADKHKTELDIDTAIKEGKLPHAVVVPGGLMGTENLAKEAAVSKLLDA
                     MHKENRLIAAICAAPVVVLSPLGILKNKRYTAYPGMEKKIAQYAGIDFQKKTENALYI
                     ADEALVQDGNLLTSNGPGSASVFAFGIIEHFAGKELAESIAKESIF"
     gene            complement(6218..7558)
                     /locus_tag="PKW26_05110"
     CDS             complement(6218..7558)
                     /locus_tag="PKW26_05110"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOQ92977.1"
                     /translation="MVILTLNCGSSSAKYQVYDWMKKEVLANGIVERVTQEGSNITHE
                     VPGKEKLNKERPCPTHKEAIELIIETLTDKEFGVIGDMAIIKAVGHRVLHGGDKFTKS
                     VIVNDEVLETFRSIKDLGPLHMPANIMGIEAAQKVLPNVPHCAVMDTAWHQTMDDAQF
                     MYATPYEWYEQYAVRRYGFHGTSFLYTAKRASVLLNKEPKDTNIIIAHIGNGASICAV
                     KNGVCYDTSMGLTPLEGLVMGTRTGDCDPALAFYIMRKTGMSVTDIDNAMNKKSGLLG
                     ITGKYTDRRDVQKALEEGDTRALLAQDMECYRLKKYFGAYIAAIGQIDAIIFTAGVGE
                     FAPAVREKACAGLEHLGIKIDHDKNWTARTKNAETCISSDDSKVKIFVIPTDEELVMT
                     EDAYALMAGSYDVHTNFTYSFQKPEYVNPARKLALQKDLESNPALASIIVEPKR"
     gene            7769..8518
                     /locus_tag="PKW26_05115"
     CDS             7769..8518
                     /locus_tag="PKW26_05115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ92978.1"
                     /translation="MQWFEKEEFWLHFGPIMFDSTKWAEAPAVAASIKSIAGLPKKAR
                     VLDAGCGPGRVSIELAALGFDVTGVDLIQPYLDAAAESAEDEGVSLNLIRADLRSFAN
                     EENAENFDCAVNLYTSFGYCNTIEEDISILKNIHATLKKDGWFIMEGLSREQAVQYFT
                     EGEWFERAGKTVLTEFSVEGAWEGLRSRWILIDDKGGRMEHSFVQRLYSAIELKRILV
                     GIGFSSCEIYGDFAFAPYNEKAKTMVLVCRK"
     gene            complement(8629..10266)
                     /gene="groL"
                     /locus_tag="PKW26_05120"
     CDS             complement(8629..10266)
                     /gene="groL"
                     /locus_tag="PKW26_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944747.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOQ92979.1"
                     /translation="MAKELLFSDDARKKMLSGVEQISKAVKVTLGPRGRLVMLDKKFG
                     APTITKDGVSVAKEVELEDAFENMGAQLLKEVASKTNDVAGDGTTTATVLAYSMVKEG
                     LKAVAAGITPIEIKRGMDKAVNLAVEEIQKNAKEVKGTEEVQHVATVSANNDSEIGEI
                     LASAIQKVGKDGVITVEESKTMETTTDYVEGMQFDRGYISSYFVTDRERMETVFDDPY
                     ILITDKKIASMKDLLPLLEKVAQSGKPLVIIAEDLEGEALATLIVNALRGTLKTCAVK
                     APGFGDRRKAMLEDIAILTGGQVITEELGLKLETAELEQLGQAKSVKIDKENTTIVDG
                     AGNSKDIKDRVSEIKAQIEESSSDYDSEKLKERLAKLSGGVAVINIGAATEVEMKEKK
                     HRVEDTLAATRAALEEGIVAGGGLALIQAALALDKTELKLSDDEKVGFKIVKRALEEP
                     MRQIAENAGVDGSVVADKAKNAKKGVGFDAAKLEWVDMIEAGIIDPAKVTRSALQNAT
                     SIASLLLTTECAITDIPEPTPPMPMGGGDMGGMGGMY"
     gene            10427..10870
                     /gene="rnhA"
                     /locus_tag="PKW26_05125"
     CDS             10427..10870
                     /gene="rnhA"
                     /locus_tag="PKW26_05125"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOQ92980.1"
                     /translation="MQNIEIYTDGACSGNPGKGGWGAVILIDGKEERLSGAELLTTNN
                     RMELSAALFALEKLQKKGIVKGTAMQLTSDSQYLKNGITTWIQSWKKNGWKTANKQPV
                     KNKDLWEALDALNAFFSIEWKWVKGHAGHHYNEICDELARKAMDG"
     gene            10971..12764
                     /gene="aspS"
                     /locus_tag="PKW26_05130"
     CDS             10971..12764
                     /gene="aspS"
                     /locus_tag="PKW26_05130"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOQ92981.1"
                     /translation="MKRTDTCGSLRGTDAGKKVILNGWVFRKRDHGGITFINLRDRYG
                     ITQAFVEPDSPQELLDIAQSLRMESCIALEGEVRLRPDSMINPDMPTGEIEIVVTSIE
                     VLSHCDILPFMIDELQKDGSPVIPNEDLRLKYRYLDLRTAQMQKHIALRHRVALATRN
                     HLASQGFFEIETPTFIKSTPEGARDYLVPSRLYPGQFYALPQSPQLYKQILMVSGFDK
                     YFQIARCYRDEDSRGDRQPEFTQIDIEMSFVDRDDVLAMTEGLMVSIFKEAMNIDLPK
                     QFQRIAYDDAIECYGTDKPELRFDMKLEDGAFMAEIGSFQVFKDALDAGGAVKALVVP
                     GVAEKYSRKLIDELEKTAKIYKARGLGWMKRGENGFEGGISKFFEGAHSTIDEKLGTK
                     EGDLLLFVADAKRKIACTALGAVRTKLGKDLNLCDPNAFAFAWIVDFPMFEWNEDENK
                     WDTAHHMFTMPQAQYHETLESDPGSVKGDLYDLVLNGYELASGSIRIHDPALQNKIFS
                     IVGFDPLEAEKKFGFLTEAFKYGPPPHGGIAPGLDRLVMLMCGQTSIKEVIAFPKNSF
                     AQNPMDGSPGFVDEAQLKELHLNIVDKNEAN"
     gene            12791..13852
                     /locus_tag="PKW26_05135"
     CDS             12791..13852
                     /locus_tag="PKW26_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOQ92982.1"
                     /translation="MKVIQEKLSDKRFMYKNIGLFLALSFFLFSSCKEATSPYKTQTA
                     LGTLCTIQLFDYGRESLYKELFARLDEIENHFSPTIASSEIAHINAASGDFPLIVSED
                     VFYLLEESLSFAKISEGFFDPTIGSLVKLWNISDFSPEKESLPTNEEIQKALFLVNYE
                     NLDLSPETSSVYLREKGMSLDLGGIAKGWAADEVVRILKKHKVPNAIVNLGGNIYAYG
                     KKENTKAWSVGIKNPFDSQGDPIIKLEVENTSVVTSGLYERFFEVDGIRYHHILDVHT
                     GYPIDNELVSVTIIDDSSMTADAYSTTLFAFGLEKSLSFANKEGIDAILITKDKKVFA
                     SDSIKDAVQILDADFVLYK"
     gene            complement(13861..14169)
                     /locus_tag="PKW26_05140"
     CDS             complement(13861..14169)
                     /locus_tag="PKW26_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HOQ92983.1"
                     /translation="MALYKEYDKTKTECTVTFELSADAAKGAEKVWIAGDFNSWSSID
                     SPMKKQKDGSFSIKIKLKAGREYQFRYLIDGKRWENDWSADKYVPAPFSFTDNSVVIV
                     "
     gene            14319..>14668
                     /locus_tag="PKW26_05145"
     CDS             14319..>14668
                     /locus_tag="PKW26_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOQ92984.1"
                     /translation="MGKRPYLFTSESVGEGHPDKLCDQISDAILDACLADDPESHVAC
                     ETFASTALILVGGEITTETFVDVQQVARDISREIGYTDSDYGLDCDSMAVLNMIHAQS
                     PDINQGVVGAGLKE"
BASE COUNT         4195 a         2756 c         2999 g         4618 t
ORIGIN      
        1 tttttaataa gtggtattac aaggcaagat tgggttgttt ttgaaggaaa ttatttttat
       61 gcaatcccag aagaaaacac aaaggtgttg cgagttgttg aaaaaccggt tgcggtgcgt
      121 ttgcttttgg aaacaaatga ttgggcacat atagcctatc cgattttaaa gacaaagagc
      181 gaagtgcaac atgcctggat aaaaaaaact gagatacgta aataattggt aatgtgaata
      241 tagaggtgaa aatggaagac ttatatgcaa tacttggtgt gtcaaaaaaa gcaagcgctg
      301 atgaaataaa aaagcaatac cgaaaacttg cacaaaaata tcatcccgac ttaaatcctg
      361 gagacagagc tgctgaggag cgatttaaaa gtatttctgc tgcctactca atcttggggg
      421 atgaagcaaa acgaaaagac tacgacttcc aacttgaaaa tccttttcaa gcaaggaatt
      481 atgcgaacca agctacttct tcgcaagatc ctttttggga atggttcaac caagaggcat
      541 tttcacaaaa aaatcgtggc tatacacagt atacttggtc aacgaataac tcaaagagcg
      601 ataaaaacga acaggtacca ctgacaaggg cagaggcaac ttctcttttc ttgcgaggat
      661 tgttttctgt acttctcgga ttattgttct ttagaatttc ttttatactc attccttttg
      721 gacccttgct ttgtatttta ggtattgcaa agggtgtttc aaatgcagca aggggaataa
      781 aggatttttt tctaagcttt aaagtaaagt aagaccaacg gcattttgca aaaaggcttt
      841 atagcatttg ccgaaaaggc tttaaagcta tttttctcta aagataattc tgcctctatt
      901 taagtcgtaa ggggaaaggg caacctttac cttatcgcca ggcacaattc taatatagtg
      961 ttttctgatt tttcccgata aatgggcgag aattacatgt ttattctgta attcaactct
     1021 aaacatagtg tttggaaggg cttcttttac aatgccttct acttcgattg cttcttcttt
     1081 ggacacaaaa cctccataaa ttgtatataa gtataacata attgtgaaaa aatgtacaat
     1141 gcacagaaat gacaaaaaat gagtaaaaaa cgataaaaat gtttgccttt tttgcgattc
     1201 ttacttatac tcatatgagt ataaatctta gtttagtttt ctttgataca tacaatatct
     1261 tacaacacga gggtataaaa tggaaaaaaa ctttattgaa aaaatgaaag aaactttgct
     1321 ggcacaaaag caagagctta taaataatct catagctaat aattcagatt ttcaaaacat
     1381 tgtcgacggt cttaccggca aagatgctgt ggatgtagca acagatgatg ttgatcgaaa
     1441 aatgcttgaa gcgcttggtg caaaagatgt aaataaacta aaactcatcg attcggctct
     1501 ttctcgcatt gagcaaaata aatatggttt gtgtattaaa tgtgggaaaa aaataccaca
     1561 agaacgtctt gaggctatac cttcagcact cttatgtatt gaatgcaaaa cagaagacga
     1621 aagaaaaaat cgctaaaggc tctgtctttc ccaacctttt ttcgttaaat aaaagagctc
     1681 cctaagtcca cgggttttaa ggagctcttt tgtgttttca aactctgagt caaggaagcg
     1741 cggactatga gaatctgaat tgagcataag cggtacccca agtgaaacaa ggtaatcaat
     1801 aaaaacagta gaaggataga cttctttgtg taaactacgt gcaagtgccg atgtatttat
     1861 ttcgactatc ttgttttgtc tttttgcttc ctttgctgtt gctaaaagtt catttttata
     1921 ccaacgagca ttttcatcat aaaaggctat ttcttgattt cgctttttta ttaaatcgat
     1981 atgaccgatt atatcaaaat ctccttgttt catcatctct ctttggagat aaaaatacgt
     2041 ttgaattgct ttttttgtgt ttccgttaaa aactctttga atgcctgaaa cgagttcttc
     2101 cgcagtgttg tccaccggaa aaaagaaatc tggaatacct gccttgtttt taattgcccg
     2161 agcatcaggg ttaaaaagat aatgcactga accaatgaga tagtctggcg aaaagtcttt
     2221 gtacaaagca taacttggct tagagagcgg tggtataaat tccgtttcaa agccatggaa
     2281 aatttcaatt ttgtctttga aatcttcttt gagactttga atttcatttg tatagtcttg
     2341 tattgttttt ggatttaagt gatattttgt cgaaaagggg tacattgcat ggcttgaaaa
     2401 gccaagatgt gtaaaacctt tttcaattgc tgctaaaatc atttctttgg tagaatagcg
     2461 cccgtcgcac caagtgctgt gggtatgata atttgtctta atttgtttta aagccatgaa
     2521 tctataacct cttgtgattg tttttcaaat tgtaaaaata aagcagaaaa gttattcaaa
     2581 ccactggtaa taacttctgt gtttatatcg tgtttttttt caattgcctg tgttttttct
     2641 gaaagcattg tgtttatata ttcttctaaa tcttctgcct gtaaagcgag agcgtgggca
     2701 gcaatagcat tcgagctccc ttttaaggca tgcgcaatag atgcaagcct ttcaaagttt
     2761 ttttgaaaga ggctttcacg tgcgttgata agggcgcttc gtgtttgctg tatgtattgg
     2821 gttatcaatt gctttccgag aaaaacatca ccagctactg tgtcaagcaa atcggcactg
     2881 tcccagcttg tttgtaaaat cttttttgtt ttactaatct gtcttggata tttttctgtt
     2941 ggaataaaac aagaactcca tttttctaaa agctttgcaa catcgttttg tttaaatggc
     3001 tttaacaaat agtcattcat gcctacttgt ttatacgtgg taaaatcttc ttcatttgta
     3061 tttgctgtgc aagcgacaat gatacccttg tatttttttt tacgtaaaaa acgtgtagct
     3121 tcaacaccgc tcataagcgg catttgtata tccataaaaa taaaggagat accacggttt
     3181 ttatttaaac aaagtatcgc ttcttttcca ttgcttgcgg taaatacttc tatttcaagt
     3241 ttttctagga aggttttgag gagtttttga tttataggat gatcttcggc aatgagtact
     3301 tgcttgaaaa gtattttttc acctgttgct tttggttttt ctatttctat tggttcaagg
     3361 tctaaggaac tgtcgctact tattgatttg agggtttcta taagcatctt ttttttcaaa
     3421 ggtttataga ggtagccgtt aaaccaatcg agcattttca ttttcgcttc cccacccatc
     3481 tgtccttcag gaaccattaa aaaaagcatg gtagtattta tattttcatc ggaattgatt
     3541 tctgcagata agcgccaccc gtccatcacc ggcatgatca tatcgatgaa ggcaatcttg
     3601 tagggctttt ttttctcagc tgcctccttg agcatatcga gagctgtttg tcctgaggtt
     3661 gcaacatgaa ttttttcaaa gcccaaaaac tgaagttttt ttgtaaaaga ttcaagacta
     3721 agtacattgt catcgacaag gagaatgcgc gtctgtgggt caagtggtat tgctggtttt
     3781 attggttttg ttgcattttg aaagatgcag ggaaggctaa accaaaagag agagcctccc
     3841 gagggattgt ctttcattcc gatttttccg ccaagcagtt ttgttaaacc ttttgaaata
     3901 gaaagtccta aaccagtccc gccaaatttt cttgtcgtag agttgtcaac ttgataaaaa
     3961 tcctcaaaaa gattttcttg tttttctttt ggaattccta tcccagagtc gcttacttca
     4021 aaaataatat ttgttttgct tgtgtcaagg aatacagaaa gatgcacata accttctttt
     4081 gtaaacttta ctgcattttt aagcaggttt aataaaattt gctgtgtacg gtgtggatct
     4141 ccttttatgc ttggaggcaa gcgataatcg atatctgtta taatttcaat tttcttgtta
     4201 tgcgcttcaa tacaaactaa gtcgacaact tgttcgcata aatcgctgag agaaaagaaa
     4261 atatgttctg tttttacttg tcctgaaata agttttgaca aatcaaggac atcatttaca
     4321 aggttcaaca agacatccga gctaaatcgt aattgccgta aatattcagc ttgttcttta
     4381 tcgagttctg tttgttgcaa tagttcagtt gtgccaataa tagattggat cggtgtacgt
     4441 agctcatgca cggttgtggt aaaaaaacgt gaggataggg gattgaatgc ttgacggaga
     4501 gacattttat atccttgagt gcaagacttc aatagctttt tgtaaaagcg cttccggttt
     4561 tagaggtttt actaaatatg attttgcccc gagactgagc gcttgtacaa cagattcttt
     4621 acttgttgca tttgaataaa taataatagg tgttttatat tgtttagagt gaagcgtctt
     4681 taatatatca aagcccgaaa gacctggcat ataaatatcg aggatgatga ggtcaaaaat
     4741 atgtttgttt gttgccatta agaattcaga acctgagtca aaaagcgaaa tttctgcatt
     4801 gatttgttta aaggtatttt gcaagattgt tcgtgttact tgatcatcat ctacaatggc
     4861 aatatgcgga ttatcattta tttttttatc gcttgaatcg aggacgccag aatcggagta
     4921 aaacttcatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ttttgttcaa
     5041 taatttcaga aatacgatat tttaaaagcg atattttttc acgatttaac ccctgggcaa
     5101 tttcaataaa aataatgtcg ttgtttagat gaagctcaag gatacaaggc gttgtatcaa
     5161 ttgaaaagcc tgttttaagc acctttccta tggattcaaa aaaaacatca aacttgatag
     5221 gtttgttaaa atattttact acattaaact gagaaagcaa ggcaatttga tcttgtgtta
     5281 tgattggtcc agtcataaaa acaggaatgc gtgatgcatt tgggtcttca tttttttttg
     5341 ccaaaaaatc aatgatttcg cgtatggata cagataggtc gatgatgagt aaatcgggta
     5401 ggcttgaaac gagctttgta aaagcatctc tttttatatg ggcgatttcc acatttactt
     5461 tttcgtgaga aagtttttct tttagaaatt ccctaaacaa aggtggtgcg tctattatga
     5521 gtacctgttt catgctataa tggtactcga aatggagtct ttttacaagt tctttattgt
     5581 ataattttat agggggaata atgaataaaa aagatgtaca tattgttatc cttcttgctg
     5641 atggatttga agaaattgaa acagttgtac cctacgattt actcgttcgt gcaggtttta
     5701 cggtaaaaac aatagcattt agtgaagata gccttgttag tggctcacgt ggcttaaaaa
     5761 tacaggcaga taagcataaa acagagctcg atattgacac tgctataaaa gagggaaagc
     5821 tgcctcatgc ggtagtagta cctggtggtt taatggggac agagaatctt gcaaaagaag
     5881 ccgcagtttc taaactttta gatgcgatgc acaaggaaaa taggctcatt gcggcaattt
     5941 gtgctgctcc agttgtggtt ttatctcccc tgggtattct taaaaataag cgttatacag
     6001 cctatcctgg aatggaaaag aagattgcgc agtatgcagg aatagatttt caaaaaaaga
     6061 ctgaaaatgc cttgtatatt gcggatgaag ctctggtaca agatggcaat cttttaactt
     6121 caaatggacc aggttcagca agtgtctttg cctttggcat catagaacat tttgccggta
     6181 aagaacttgc tgaatcaatc gcgaaagaaa gtatttttta gcgttttggc tcaacaatga
     6241 ttgaggcaag cgcaggattg ctctctaaat ctttttgcaa ggctaatttt cgtgctgggt
     6301 ttacgtattc tggtttttgg aaggagtatg taaagtttgt atggacgtcg tagctgcctg
     6361 ccataagggc ataggcgtcc tcggtcataa caagctcctc atcagttgga atcacaaaga
     6421 tttttacctt cgagtcatca cttgaaatac aggtttctgc attttttgtg cgtgcagtcc
     6481 agtttttatc gtggtctatt ttaatgccaa gatgttcaag ccctgcacaa gctttttcgc
     6541 gaacagctgg tgcaaactcg ccaacacccg cggtaaagat aatcgcatcg atttgtccaa
     6601 tggccgcaat ataggcgcca aagtattttt tcaatcgata gcattccata tcttgtgcca
     6661 gaagtgcgcg tgtatcacct tcttcaagag ccttttgcac atctcgtcgg tcagtgtatt
     6721 ttccagtaat accgagtaag ccagattttt tattcattgc attatctata tcggttacag
     6781 acatgccggt ttttctcata atgtaaaagg cgagtgccgg gtcgcaatct cccgttctcg
     6841 tgcccatcac aagcccttca agaggcgtta aacccatgct tgtgtcgtag caaaccccat
     6901 ttttaaccgc acaaatagaa gcaccattcc caatatgcgc gataataata tttgtatcct
     6961 ttggttcttt gttaagaagt acacttgctc gctttgcggt atataaaaaa cttgttccgt
     7021 gaaaaccgta acgacgaacc gcgtattgct cataccattc gtagggagtt gcatacataa
     7081 attgtgcatc gtccatggtt tgatgccagg cggtatccat cactgcacaa tgcggcacat
     7141 ttggaagtac cttttgcgca gcctctatgc ccataatatt tgccggcata tgaaggggac
     7201 caaggtcttt gattgaacgg aaggtttcca gcacctcatc gtttacgata accgactttg
     7261 taaacttatc tcctccgtgt aagacacgat gccccaccgc cttaataata gccatatccc
     7321 cgataacgcc aaattctttg tcagttaagg tttcgataat aagctcgatc gcttctttat
     7381 gggtcggaca gggacgctcc ttattaagct tttcctttcc tggaacctca tgcgtgatat
     7441 ttgagccctc ttgggtaaca cgctcgacaa taccgtttgc caaaacctct tttttcatcc
     7501 agtcatagac ttgatacttc gccgatgacg aaccgcaatt taacgttaaa ataaccatga
     7561 tacatcctta ttataataaa aaattgatac aatgagttta gcatattttt ctgccactgt
     7621 catgcggtga aaaaactcag ccttagcctg ttgttttttt gacaggattt acatgaaaaa
     7681 tgtatcttat ctctttataa aaactatttg tgcggatata gtttttatgc tactatgaga
     7741 gtatatcaaa tttgttaggg aggttcttat gcaatggttt gaaaaagaag aattttggct
     7801 tcactttggc cctattatgt ttgactctac aaagtgggct gaagcacctg ctgtggcagc
     7861 gagcataaaa agtattgcgg ggcttccaaa aaaagcaagg gttctagatg caggctgtgg
     7921 tcccggtcga gtgagcatag aacttgccgc cctaggcttt gatgtaaccg gtgtagacct
     7981 catacagccc tatttggatg cagcagctga aagcgcagaa gatgagggcg tatctcttaa
     8041 tttaattcga gccgatttac gcagctttgc aaatgaagaa aacgccgaaa actttgattg
     8101 tgcggtgaac ctttatacct cctttggcta ctgcaataca atagaagaag atatatcgat
     8161 actgaaaaat atccatgcga ccctaaaaaa agacggctgg tttattatgg aaggtctttc
     8221 tcgcgaacag gcagtccagt attttaccga gggagaatgg ttcgaaaggg caggaaaaac
     8281 tgttttaaca gagtttagcg tcgaaggtgc ctgggaaggc ttgcgctccc gctggatatt
     8341 gatcgatgat aagggcggca gaatggaaca tagctttgtg caaagactct attctgcgat
     8401 agagctgaaa cgaatcttag tcggtattgg cttttcttcc tgtgaaattt acggcgactt
     8461 tgcctttgcc ccctacaacg aaaaagcaaa aactatggtc ctcgtttgca gaaaatagag
     8521 agctttggtt ttttcgagca gtctttcata atagctataa aaaaagctag ggtttttgcc
     8581 ctagctcttt ttatcgggtt ttaccgaaac cttatctggt aaaggttttt agtacatgcc
     8641 acccataccg cccatgtctc cgccgcccat tggcattggt ggagttggtt ctggaatgtc
     8701 agtgattgca cattcggtgg tgagaaggag gcttgcaatt gaagttgcgt tttgcaatgc
     8761 gcttcgggtt acctttgcag ggtcgataat tcctgcttca atcatatcta cccattcgag
     8821 ttttgcagcg tcaaagccaa cacctttttt cgcgttcttt gccttatcgg caacgacgga
     8881 accatcaaca ccggcatttt cggcaatttg tcgcatgggt tcttcgagtg cacgttttac
     8941 aatcttaaag cccacttttt cgtcatcgct tagtttgagt tctgttttat cgagggcgag
     9001 ggctgcttga atgagggcaa gaccaccacc tgcaacaatg ccttcttcga gggctgcgcg
     9061 agttgcggcg agggtgtctt ctactcggtg tttcttttct ttcatttcaa cttcggttgc
     9121 tgcaccgata ttgataacgg caacaccgcc agagagtttt gcaagtcgct ctttgagttt
     9181 ttcgctgtcg taatctgagc ttgactcttc gatttgtgcc tttatttcgc tgactctgtc
     9241 ttttatgtct tttgagtttc ctgcaccgtc aacgatggtt gtattttctt tgtcaatttt
     9301 tacgctcttt gcttggccga gttgttcaag ttcagcggtt tcaagtttaa gcccaagttc
     9361 ttcggtaatt acttgcccac cggttaatat tgcaatgtct tcgagcattg cttttcgtct
     9421 gtcgccaaag ccaggcgcct ttaccgcaca ggttttgagt gtgccacgaa gtgcgtttac
     9481 gatgagggtt gccaaagctt caccttcaag gtcttcagca ataataacta agggtttacc
     9541 tgattgtgct actttttcaa gcaagggaag gaggtccttc atgcttgcta tttttttatc
     9601 ggttataaga atatagggat cgtcaaaaac agtttccatt cgttcgcggt cggtaacaaa
     9661 ataagatgag atatagccac gatcaaactg cattccttct acatagtcgg ttgttgtttc
     9721 cattgttttg gattcttcaa cggtaataac accgtctttg cctacttttt ggattgcgct
     9781 tgcaagaatt tcacctattt ctgagtcatt gtttgcagaa acagttgcaa cgtgttgtac
     9841 ttcttctgtt cccttcactt cttttgcatt tttttgtatt tcttcaacag cgagatttac
     9901 agccttgtcc ataccgcgtt taatttcgat gggggtaatg cctgctgcaa cggcttttaa
     9961 gccttctttt accattgaat aggcgagaac ggttgctgtg gttgtaccat ctcctgcaac
    10021 atcgtttgtt tttgaagcaa cttctttcag aagctgtgcg cccatgtttt caaatgcatc
    10081 ttccaattct acttcttttg caacggaaac gccgtctttg gtaatggttg gggcaccgaa
    10141 ttttttatcg agcataacga gtcgcccacg tggtccgagg gtaactttca ccgcctttga
    10201 aatttgctca acaccagaga gcattttttt tcgtgcatca tcgctaaaaa gcaattcttt
    10261 tgccatagtt tctaactcct tatttttttg gacaagtctt ttaaaatatg atagacttgt
    10321 ttccataaga tagatattta ttggcttggg agggtcccaa tgaagccata tagtaaaaag
    10381 atacacatat tttttaaaaa gggcaagtga aaaaaggaat tctaaaatgc aaaatattga
    10441 aatttatacc gatggtgcct gttctggaaa tcccggaaag ggtggctggg gggcagttat
    10501 cctcatcgat ggcaaagaag agagactttc tggagcagaa cttttaacaa caaataatcg
    10561 catggaattg tccgcagcgc tttttgcctt ggaaaaactt caaaaaaaag gcattgtaaa
    10621 ggggacagca atgcagctta cgagtgatag tcagtactta aaaaacggca tcacaacctg
    10681 gattcagtct tggaaaaaaa atggctggaa aacagcaaat aaacagcccg tcaaaaacaa
    10741 ggatttgtgg gaagccctcg atgctttaaa tgcctttttt agcatcgaat ggaagtgggt
    10801 aaaggggcat gcggggcatc attataacga aatttgtgat gaacttgcta gaaaggcaat
    10861 ggacggctag aacgagattg tttgcgtggc aagctactca cagagattgc gtttttttat
    10921 cactacttgc atagcttttt gtgaattgct attatataaa aatgaacaga atgaaacgaa
    10981 cagatacttg cggctcccta cgagggacag atgcaggaaa aaaagtaata ttaaatggtt
    11041 gggtttttcg aaagcgtgac cacggcggca taacctttat caatttacga gatcgctacg
    11101 gcataacgca agcctttgtt gagcccgatt ctccacagga gcttttagac attgcacaaa
    11161 gtctccgcat ggagtcttgt atcgcacttg aaggagaggt tcgcctacga cccgattcga
    11221 tgataaaccc cgacatgcca actggtgaaa ttgaaatcgt tgttacatcc atcgaggttt
    11281 tatcgcattg tgatattctt ccttttatga ttgatgagtt gcaaaaagac ggcagccctg
    11341 ttattccaaa cgaagacctt cgcctcaagt atcgctatct tgatttacgt accgcacaaa
    11401 tgcaaaagca tattgcgctt cgccataggg tagcccttgc aacaagaaat catcttgcaa
    11461 gtcaaggttt ttttgaaatt gaaacaccga cttttataaa atccacccct gagggagcgc
    11521 gcgactacct tgttccttct cggctctatc ccggacaatt ttacgcactt ccgcagtccc
    11581 cacagttgta caagcaaatt ctcatggtca gcggctttga caaatatttt caaattgccc
    11641 gctgttatcg ggatgaggat tcacgaggcg acaggcaacc agaatttacg caaatcgata
    11701 tagaaatgtc ttttgtagac cgtgacgatg tgcttgctat gactgagggc ttgatggttt
    11761 ctatttttaa agaagcaatg aatattgacc tgccgaaaca atttcaacgg atagcctacg
    11821 acgatgcaat agaatgttat ggaaccgata aacctgaatt acgcttcgac atgaagctag
    11881 aagatggcgc ctttatggca gaaataggta gttttcaagt tttcaaagac gcactcgatg
    11941 caggtggtgc tgttaaagcc ctcgtcgtgc caggagttgc agaaaaatac tcgagaaagc
    12001 tcatcgacga gctagaaaaa acagcaaaaa tctacaaggc gcggggtctc ggctggatga
    12061 agcgagggga aaatggtttt gaaggcggca tttcaaagtt ctttgaaggt gcgcattcta
    12121 caatcgatga aaaactcggt acaaaggaag gcgacctcct cctctttgtt gccgatgcaa
    12181 agcgaaagat agcctgcacc gctttaggag cggttcgaac aaagcttggg aaagacttaa
    12241 acctctgcga cccaaatgcc tttgcctttg cctggattgt cgacttccct atgtttgaat
    12301 ggaatgaaga tgagaataaa tgggataccg ctcaccacat gtttactatg ccacaagccc
    12361 aatatcacga aaccttggaa tcggatcctg gctctgtaaa aggcgactta tacgaccttg
    12421 ttttaaacgg ctatgaactc gcatcaggct ctataagaat acacgaccca gctctacaaa
    12481 acaagatttt ttccattgtt ggctttgatc cccttgaggc agaaaagaag tttggctttt
    12541 taaccgaagc ctttaagtat ggtcccccac ctcatggcgg tattgcacca ggtttagaca
    12601 ggcttgtaat gctcatgtgt gggcaaacct ctataaaaga agttatagcc tttccaaaaa
    12661 actcttttgc acaaaacccc atggacggtt ctcccggctt tgttgacgaa gcgcaactca
    12721 aagagcttca cttaaacatt gtcgataaaa acgaagcaaa ctaagcttcc tttaggcgaa
    12781 ggagattatt ttgaaagtca ttcaggaaaa actgtcggac aaaagattca tgtataaaaa
    12841 cattggctta tttttagctc tgagcttttt tctttttagc tcatgcaaag aagcgacaag
    12901 cccatataaa acccaaacag ccttgggcac cctctgtaca attcagctct ttgactatgg
    12961 aagagaatct ttgtacaaag aactatttgc acgtcttgat gagatagaaa accattttag
    13021 tccaacaatt gcttcttcag aaatagctca tataaatgct gcttcaggag attttccgct
    13081 tattgtaagc gaagatgttt tttaccttct tgaagagagc ctttcttttg caaaaatctc
    13141 tgaaggtttt tttgacccga ccattggttc cttggtaaag ctatggaata ttagcgattt
    13201 tagccctgaa aaagagagcc ttcctacgaa tgaggaaata caaaaagctc tgttcctcgt
    13261 taactatgaa aacctagacc tttcacctga aacttcttct gtctacttga gagaaaaagg
    13321 tatgagcctt gatttagggg gcattgcaaa aggctgggct gccgatgaag ttgtaaggat
    13381 tttaaaaaaa cacaaggttc ccaatgcgat tgtgaatctc ggtggtaata tttatgccta
    13441 tggaaaaaaa gaaaatacaa aggcttggtc tgttggtata aaaaatccct ttgactcaca
    13501 aggcgacccc attatcaagc ttgaagtaga aaacacaagc gtcgtaacat cgggcttgta
    13561 cgagcgcttt tttgaagtag atggcattcg ctaccaccat atcctcgatg tgcacactgg
    13621 ctacccaatc gataatgaac ttgtttcagt taccattatc gatgactcct cgatgacagc
    13681 cgatgcgtat tcgacaacgc tttttgcctt tggcttagaa aaatccctta gctttgcgaa
    13741 caaagaggga atcgatgcga tattgattac aaaggataaa aaagtatttg cctcggattc
    13801 tattaaagat gcggtgcaaa tacttgatgc ggactttgtc ttatataaat aagtctttat
    13861 ttagacgatg acaacggagt tgtcggtaaa agaaaagggt gctggaacat acttgtcggc
    13921 actccaatca ttttcccatc gttttccgtc gattagatag cgaaattgat attctcttcc
    13981 tgctttcagt tttattttaa tagaaaagct gccgtctttt tgctttttca tagggctatc
    14041 gatactgctc caactattaa agtctcctgc gatccatact ttttcagcac cttttgctgc
    14101 atctgcagat aattcaaagg ttaccgtaca ctcggtcttt gttttatcgt attctttgta
    14161 taaggccata tttcctccat gaaaaaatca aaagtattgt tcaatgtcta aagtatcgag
    14221 cggatatatg caactacttg tcattgatac gctaacagta tactatatta aaactatttt
    14281 gtgtagttaa attatatctt ttgcaaaagg aatagtgaat gggaaaaaga ccatatttgt
    14341 ttacttcaga atctgttgga gaggggcatc cagataaact ctgtgatcaa atttcggatg
    14401 caattttaga tgcttgttta gctgatgatc cagaaagcca tgttgcttgt gaaacctttg
    14461 cgtctacggc attgatattg gttggtggtg aaattaccac agaaactttc gtagatgtac
    14521 aacaagttgc tcgtgatatt tcacgagaga ttggctatac tgactccgat tatggtttag
    14581 attgtgattc tatggctgtt ttaaatatga ttcatgcaca atcgccagat ataaatcaag
    14641 gtgttgttgg ggccggctta aaggaatt
//
LOCUS       DAOSSW010000045        14444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_4263_length_14444_cov_6.522413, whole genome shotgun sequence.
ACCESSION   DAOSSW010000045 DAOSSW010000000
VERSION     DAOSSW010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 14444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14444
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4263_length_14444_cov_6.522413"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PKW26_05150"
     CDS             complement(<1..273)
                     /locus_tag="PKW26_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92985.1"
                     /translation="MKKNLIFKLCFIVGIFLIILTACDLGTKQWANITFNLEGPAIEQ
                     ARANSGASSSARYIHPYASSLKFELFSGKELLDSQTISIQSGTKSIS"
     gene            complement(416..1765)
                     /locus_tag="PKW26_05155"
     CDS             complement(416..1765)
                     /locus_tag="PKW26_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOQ92986.1"
                     /translation="MIYFFFALIVSFSISIFFLFRYRDRAYYERFSGQKNWWWLWDFF
                     FLKQKKVGKPFCFAHFDIKDFKMINENFGHIIADKVLARVGEVAEKEPWVKHLVRCHN
                     DNFALVTELLSEKELKTKLLEMFDKMDSLPEDPSYKIYYRCGAVSSEHSIAGKDMVAD
                     FAKLAQAQGIKPSATEVIIYTESMKDKYFKAKKIVREIPRALKDKELIVYIQPKHNIK
                     GRLSGGEALIRWNRHFNEFLFPRSFIGYVEEAGLIHLIDFYVLETVCKKIKEWRSQNI
                     ALVPVSVNISRYTITIPFFLEKALQVVDSYDVPHELIEFELTETALYDNQEYIVSIME
                     AIKTEDFKLSMDDFGTGYSSLNLLQKMPLDTLKIDKSFVDNILTSIEADEIFKAQVIL
                     KNILSMTKDMNVDSLAEGVELEKQKNILERWGCTHIQGHYYNKAMPIYDFEEMLQKK"
     gene            1996..2955
                     /locus_tag="PKW26_05160"
     CDS             1996..2955
                     /locus_tag="PKW26_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOQ92987.1"
                     /translation="MIKHVLAPLDGSVLAESVLPHIMSISLVLNAQVSLLHVIERNDK
                     DWAHAIDPIDWHVRKDKAKKYIEEKAKPFIKKGLKVHTILKEGNPAEHIINYTYENDV
                     DLVIMSSHGQSGISDWNIGSVVQKVMMGIPCSTLLVRASHTEEQVECLKYKTIFIGLD
                     CSPQAEFVLPLAGSIAEHYKAKLVLGTVIKKVELLNHLPFSHEEEKLLEKVLDLSKKE
                     AQSYLEKIKTQLEVKNLHVETRLGIHNNISSALMDMVDEVKADLVLLSAHGHSSEGRH
                     SFGSMATSFIVYGNKTSLIMQNLPYNRPKPVQSILSKRGSKEF"
     gene            2958..11465
                     /locus_tag="PKW26_05165"
     CDS             2958..11465
                     /locus_tag="PKW26_05165"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOQ92988.1"
                     /translation="MNEKQLTEKPCDMSASYLATNMEHESKKKQPCPLNPWTAELEKT
                     IKKAYEFFEEASRKKKTVPQAAEWLLDNYYIIEEAFAQFKENIPLSFYKKLPIAKVQE
                     SPCLPRIYFLSAGILLTNQGRLDIEGIKTYVNEFQKTIPLTIGELWAIPTMMRFKILE
                     GLASSLSILIDKKYESKNVFPFSFENAEKEIAFLEAADYVSVFILSLRMIANQDWKVF
                     FEDCSLVEKKLRKDPAGFYDKMDFATRNSYRNEIEELGKGSALAEIEAVEKCLELVEK
                     GSDFQERTVGYYLIDLGREELEKELAYKPAAKKKLKHWLKKRASAFFLLTNFFGSLFL
                     SASLAFICWLFGAKIAEVFFAFFLSLVPLSAFVSVVVNRFVLRRVSPKILPKLNLSEG
                     VPEEFLSIVVIPTLVGSEADVEMLLRKIETQYLGNIDRNIYFALLTDFTDAKSESLST
                     DEAILTRLTEGIDQLNETYSEGDYKPFFVFHRKRLFNKSENCFMAWERKRGKLEEFNR
                     LILGDANTSYYVKHGDVGVLPKIRYVITLDEDTLLPNGSAKRLIGAMAHPLNRAVFDE
                     KTGQLIKGYSIIQPRLQVWPIEANYSLFTQVYSGDLSLDLYTLAVSDVYQDLFREGSF
                     VGKGIYDVVAFEKSLENKIPENRLLSHDLFEGIQGRCALASDIVLYEGYPSHYVAYMK
                     HSHRWIRGDWQLVPWLKKRVRGRDGKKYKNTISRINRYKIFDNLRRSLIPFFVMLLLF
                     ASWFYLPGPKILWIFLALTPYLPSIFFSFIDSLFGPKESDGPRGDVKINRQAFLRTFF
                     ELSFLPHKTFVSLDAIIRTLWRLSFSKKHLLEWVSAAQNEKRYGKKDMASIILKVFFP
                     TQVFSLLVFFVAFFFAPTQIFYLLPFVLLWFFAPFIAIRIGKAQEHKTEKLTENQDRL
                     LRRLSRNTWLFFEHFVGPEDNWLPPDHYQESPRGIVAHRTSPTNIGLLLLSSLSAFDF
                     AYFGHLELYTRLQNCFDGMKGLERYRGHFLNWYDTKSLSPLNPRYVSSVDSGNLAASI
                     MIAAQAPKDVLQKPVIRLAGLLDSLDLLFDFSRSLSLPKKSEDLEKNLLALMKNIQAI
                     DEEDSDFPVQLKKLLVDEPKKLEEGLEKLIHKSRQEIDTNLLQSLSTWVDRCLRHMRN
                     VQLDIERYIPWTFAFADIPEELEAVLSNNEISDLWLRLEKTLVFNPPFEAIPKITVNA
                     LIILDDLMPLVKTEIFVSWFENLREKIQESHIASRSLISDFSEFSKTADLLVREMDFS
                     FLFNTQRKVFHIGYNLDSGQMDENYYDLLASEARIASIVAIGKGDVPSSHWLHMARPL
                     TKHAGKQLLLSWSATMFEYLMPLLFMKTYENSLLSQSCTLAVEKQIAYGKEKGTPWGI
                     SESSYYHFDASEVYQYRAFGVPGLGYKRGLGEDHVIAPYASILALPIAPKAVLQNLMQ
                     LKKKEAWGFYGLIESLDFTKRRLQPGQNFALVKTYMAHHQGMILLALSNYFNNNKHIA
                     RFHADPRIKSLEFLLQEQNPKSARVEHPHPQSLGLVHPIKSIIPMESWPIISSLRQPQ
                     LNMLTNGNYTLMISASGAGFSRWKGTDLTRWRGDASLDNHGSWIYLKDLDSNKLWSPT
                     AQPMGGNIEKSQVSFYPHKAVFEENLNEVHTKMEVAVFQDADAELRYMELTNANNERR
                     NLSLTSYGEVILTSQEADSRHPAFNKLFIESEYIEDYNALLIKRRPRSKDEKTLYFIH
                     LVQGEKDDFSVHSYETDRSVFLGRGGSIQNPHALQDEEKLTKTLGFSLDPIYALQTKL
                     TLKPYETKKIAFISLVAETKKEALRLASRFQQWYGIEKSREMLSLYAEEEIERFELSI
                     ENYQLNAKMLSALLYPSQAMRANAQILRKNKLGQAGLWPFAISGDYPILVLKLSKNGT
                     ELLQELLKSHSFWRRRGVKVDFVILNQRISVYEQELSNSIYRLVNQSGNEGMINQRGG
                     IFILREDQMNEDEKLLLYASARLILEGEEGSLEKQLEKFALETMRLPRFVAVKPPYWL
                     KEGGHLISRPENLAFDNGYGGFSSDGRAYSIYLEGYEQTPAPWINVIANKDFGFLVSE
                     RGLGTTWALNSGENRLTTWHNDPVLDSPSEALYLRDEDTGQIWSPTPGPKRDGAPYLI
                     RHKPGASIFEHSSYGIVQKLRLFADVEKPIKMVQLQISNKTEETRRITVSYYAEWVLG
                     DLREKHAAYIIPEFDSKHSALLVQNPYNVQFASRVAFLASTRELNWVTSDRLEFLGAR
                     KSYEEPSALSRLGLTGNVSPGFDPCAVVQNLIWLAPGETKEVTFLLGQGEDRDNAIEL
                     IQEFQDIQNIEKAYRRSIKKWEEILGTIEVKTPEKTMDIMLNTWLLYQSLSCRMWGRT
                     AFYQSSGAYGYRDQLQDSMAFLHSSPDIAREHILRSAASQFEEGDVLHWWHPPTTRGI
                     RSRIVDNLLWLPFVTARYISVTGDMSILDEEIPFLHASLLEENEHERFNVFAHGDESA
                     SLYEHCLRAIRKGATKSAKGLPLMGGGDWNDGMNRVGKDGKGESIWMGFFLIACLKNF
                     AEVCLEKNDSEEAEKFKSMAKEYTKAIETHAWDGAWYRRAYFDNGDPLGSSENEECRI
                     DSISQSWAIISGAAEKERAEMAMASLDKELVLNEEKILLLLRPPFNNSKNDPGYIMAY
                     PPGIRENGGQYTHGVLWALWALAKIGKGDRAFELFNMINPINHARQKADAKLYRVEPY
                     VLSADVYGAHPHLGRGGWTWYTGSAAWMYQIGIEQLLGIQRERDELIIEPSIPRHWPG
                     FEIKYRFGKSLYKIEVVQKSNTGKTVKEEERSLSLDGKELEGNSVALVDDKKEHRIKL
                     IIKTVDR"
     gene            11615..12721
                     /locus_tag="PKW26_05170"
     CDS             11615..12721
                     /locus_tag="PKW26_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92989.1"
                     /translation="MNNVLLFKKKIVCIVLFLSVYFFNGFAQDTFLLYMYSQVNDHVE
                     IKNVSAINRILINNKHSRDYDKLENYILMKSREQLVMNNLDLAMDLCYIVIDNNLDNF
                     EAVSLYTSIERTKREKEERERIEKEKAQIESIKQAETIKKDTEKIQREYQTITNTASG
                     QRVYLDQDVDDYFQTVTWGLGLGLASFSAIIDIPQVEGFYGISVLGNVFYRSEVIHAG
                     IDVFAAMALSSFSDLDFLPAKAEANLNFAFPKLAKYLYYRVGVVGFLSYLGEESFLPK
                     QFLTPTAGIALKDIGTEAFLFGLNVDYHLAHLYNAEIGPGLSAGLNFFIRFADLKKAD
                     VGLIIGLEDSFFYTSNGFQNHAKINLSIGVWNND"
     gene            12714..14138
                     /locus_tag="PKW26_05175"
     CDS             12714..14138
                     /locus_tag="PKW26_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOQ92990.1"
                     /translation="MIKKSAFIVIFFCSFFFLNAQSSLVERYLAAANTSYSEKDYAKA
                     FSYINYVYGQYDADKTPRNVEVLAELIYYDYLNHMRVTNNFSEFQVVKDRLAEFPAIS
                     SDRVTRLVRTLNTLESQNTNLETTVSTVLDLRDAVEYKIQLERTKAELHALEKALEDA
                     RESAEKEKLYRDKEFLETQKELYEKALNLSNERDGVNKTFVLLIIIAVVVLFFVLFVV
                     MMIIVRISTKNAKKQQEQFEATLQMVSQIARNPSERLSLGSFVDSFGDAASNGRIGGT
                     QASLPRPELTEEERLELQDLALKCEAVGEDIDRVTGRKNNSKNVAELVYKISHQLSCS
                     TYDSMLYFCAAMVYDAGFLKIDTEIFTKESLSTEEKYEIRSHVKQATESFDFIPEKYL
                     PVFLDAALMHHENMDGSGYPTGARGENIPLIARIIRVVESFISLISRRNYRGIFDKEA
                     AITELKNSSHLYDSAIIDALDTII"
     gene            14236..>14444
                     /locus_tag="PKW26_05180"
     CDS             14236..>14444
                     /locus_tag="PKW26_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92991.1"
                     /translation="MKSKNVKQKYKIILLNLFLLSVFSCTMNGENPFSTKKYLSIQYP
                     PENALIRNSFTMNGRILTNKKLKSI"
BASE COUNT         4511 a         2508 c         2989 g         4436 t
ORIGIN      
        1 ggaaatgctt ttagtgccac tttggataga aatagtctgt gaatccaata gttctttgcc
       61 agagaagagt tcaaatttta gggagcttgc atacgggtgg atatagcgcg cagagcttga
      121 ggcccctgag tttgctctgg cctgctcgat agccggaccc tccaggttaa aggtaatatt
      181 tgcccattgt tttgtgccaa ggtcgcaagc tgtaagtatg attaaaaaga tgccaacgat
      241 aaaacagagc ttaaaaataa ggtttttttt cattttgttt ctcctattgt ttttggttaa
      301 attcattgta taaggtactc gtgaggttct gagttgtgta actgatgtac atttttactt
      361 ttctagctct attataacac aggttttgga aaaaaccagt ttttttgaaa atagtttatt
      421 ttttttgtaa catttcttca aaatcataga tgggcatcgc tttattgtag taatgccctt
      481 gtatatgggt gcaaccccag cgttcaagga tgtttttttg tttttctagc tcaacacctt
      541 ctgcgagact gtcgacgttc atgtcctttg tcattgacaa gatatttttt aagataactt
      601 gggctttgaa aatttcatct gcttctatag aagtgagaat gttgtcaaca aagcttttat
      661 cgatttttag ggtatcgaga ggcatttttt gcaaaagatt tagcgaagag tatcctgttc
      721 caaaatcgtc catcgagagc ttaaagtcct ctgttttgat tgcttccatg atagatacaa
      781 tatactcttg attatcgtat aaagctgttt cggttagctc aaattctatc agttcatgtg
      841 gaacatcgta ggagtccaca acttgcagtg ctttttccaa gaaaaaagga atcgttatgg
      901 tgtagcgaga aatatttaca gatacaggaa caagggcgat gttttgtgat ctccactcct
      961 ttattttttt acaaaccgtt tccaaaacat aaaaatcaat caagtgaata agcccagctt
     1021 cttccacata gccaataaat gaacgtggaa ataaaaactc gttaaaatgc ctattccatc
     1081 gaatgagggc ttctcctcca gaaagcctac cttttatatt gtgtttgggt tgaatataga
     1141 caatgagctc tttgtctttg agagcacgag gtatctctcg tacaattttt tttgccttaa
     1201 aatacttgtc tttcatcgac tctgtgtaaa taataacttc agttgcgctt ggtttaattc
     1261 cttgtgcttg tgccagtttt gcaaaatctg caaccatatc tttacctgct atagaatgtt
     1321 cggatgaaac agcaccacag cgataataaa ttttatagct cgggtcttca ggtaaagagt
     1381 ccatcttgtc aaacatttcc agtagttttg ttttgagttc tttttcagat aagagttcag
     1441 taacaagggc aaagttgtca ttgtgacagc gcaccaagtg ttttacccat ggctcttttt
     1501 ctgcaacttc tccaactcgg gctaaaactt tatctgcaat tatgtgtccg aaattttcgt
     1561 ttatcatttt aaagtctttt atgtcgaaat gtgcaaagca aaaaggtttg cccacttttt
     1621 tttgcttcaa aaagaaaaaa tcccaaagcc accaccagtt tttttgacca gaaaatcgct
     1681 cgtagtaagc tctgtccctg tatcgaaaga gaaaaaagat agagatagaa aatgaaacaa
     1741 tcaacgcgaa aaagaagtaa atcataacta agattctatc attctttttt tttgaatgca
     1801 acttaaaaaa aaatgcaaaa cgagcttctg tgtctgttct gtgactttct aaatcgcttg
     1861 caatatattg aaaacctgtt aaaataatag ctatgatata gtagaataag taaaaaaaat
     1921 gattcagtag caagataatt atacatctta tgtaggagga aaatagcata aacagtattt
     1981 attgtggagg ttttaatgat taaacatgtt ttagcgccac ttgatggctc ggttttggca
     2041 gaatctgtgt tgccgcatat tatgtcaatt tctttggttt tgaatgctca ggtgagtctt
     2101 ttgcatgtta ttgaaagaaa cgataaagac tgggcacatg caatagatcc cattgattgg
     2161 catgtaagaa aagataaggc aaaaaaatac attgaagaaa aagcaaaacc ttttataaaa
     2221 aagggtttaa aagtgcatac cattctaaaa gaaggaaatc ccgcagagca tattatcaat
     2281 tacacctacg aaaatgatgt agatcttgtc attatgagtt ctcatggaca aagtgggata
     2341 agtgattgga atataggttc tgttgtacaa aaggttatga tgggtattcc ttgttcgaca
     2401 ttgctcgtga gggcttctca tactgaagaa caagtggagt gcttaaaata caaaacaatt
     2461 tttatcggtt tagattgctc acctcaggct gaatttgtct tacccctcgc aggaagcatc
     2521 gcagagcatt ataaggcaaa acttgttcta ggtactgtta taaaaaaagt tgagctctta
     2581 aatcatcttc cattttcaca tgaagaagaa aaacttctcg aaaaggtgct tgatttaagc
     2641 aaaaaagaag cccaatccta tcttgaaaaa ataaaaacac agctagaagt aaaaaacctg
     2701 catgtagaaa cccgccttgg aatacacaac aacataagtt ctgcgcttat ggatatggtt
     2761 gatgaggtga aagctgatct tgtcttgctt tcggcacatg ggcattcatc agaaggaagg
     2821 cattcttttg gaagcatggc gacgagtttt attgtatatg gaaataagac aagtttaatt
     2881 atgcaaaatt taccctacaa taggcccaaa cctgttcaaa gcatactatc aaaaagagga
     2941 agtaaagaat tttaagaatg aatgaaaagc aattgactga aaaaccttgt gatatgtcgg
     3001 catcatattt ggcaacaaat atggagcatg aatctaaaaa aaaacaacct tgtcctttaa
     3061 atccatggac agctgagctt gaaaaaacaa taaaaaaagc atatgagttt tttgaagaag
     3121 caagtcgaaa aaaaaagact gtgccacagg ctgcagaatg gcttttggat aattattata
     3181 taatcgaaga agcctttgcc cagtttaaag aaaacatacc tctttctttt tataaaaaat
     3241 tacctattgc taaggtgcag gaatcgccct gcttgcctcg aatttatttt ctttcagcag
     3301 gaattttgct tacaaatcag ggacgtctcg atatagaggg tataaaaacc tatgtgaatg
     3361 aatttcaaaa aacaattccg ctgacaatcg gggagctttg ggctataccg actatgatgc
     3421 gctttaaaat tcttgagggg cttgcgtctt ctctgtctat acttatagat aaaaaatatg
     3481 aaagcaaaaa tgtttttccc ttttcctttg aaaatgccga aaaagaaatt gcgtttttag
     3541 aagccgcaga ctatgtaagc gtttttattt taagtttacg tatgatagca aaccaagact
     3601 ggaaagtttt ttttgaagac tgtagccttg tcgaaaaaaa actacgaaaa gatccagcag
     3661 gtttttatga taaaatggat tttgctacaa gaaatagcta tagaaatgaa atagaagaac
     3721 tggggaaggg ctcagctctt gcagaaatag aggcggtaga aaaatgtctt gaactcgttg
     3781 aaaaaggaag tgattttcaa gaacggactg taggttatta tcttattgat ttgggaaggg
     3841 aagagcttga aaaggaactt gcatataaac ctgccgctaa aaaaaagcta aaacactggc
     3901 tgaaaaaacg ggcgagtgct tttttccttt taacaaactt ctttggaagt ctttttttaa
     3961 gcgcgtccct ggcttttata tgctggcttt ttggcgccaa aatagcggaa gttttttttg
     4021 cattcttttt atcgcttgtg ccgttatctg catttgtttc cgttgtggta aatcgctttg
     4081 tgttgcgacg ggtaagtccc aaaatattac ccaagcttaa tttgagtgag ggggtaccgg
     4141 aagagtttct gtccattgtt gttattccga ccttggttgg aagtgaagct gatgtcgaaa
     4201 tgcttttgcg aaaaatagaa acgcagtacc ttggcaatat cgatagaaat atctactttg
     4261 ctcttttaac agactttacc gacgcaaaaa gtgaaagtct ttcaactgac gaagctattc
     4321 ttacacggct gacagagggg atagaccaac tcaatgaaac ctacagtgaa ggagactata
     4381 aacccttttt tgtttttcat agaaagcgac tttttaataa aagtgaaaat tgttttatgg
     4441 cctgggagcg aaaacggggg aaacttgaag agtttaatcg tcttattttg ggcgatgcaa
     4501 atacaagcta ttatgtaaag catggcgatg tgggtgtttt accgaagatt cgctatgtaa
     4561 ttaccctcga tgaagatacg cttttaccaa atggaagtgc aaaacgactc ataggtgcta
     4621 tggcacatcc tttaaatcga gcggtatttg atgagaaaac gggacagctt ataaaaggct
     4681 attctattat acagccgcga ctacaagtct ggcctattga agcaaattat tcgcttttta
     4741 cacaggtgta ttctggagac ctttctttgg acctttacac cctcgctgtt tcagatgtgt
     4801 atcaagattt atttagagaa ggaagttttg tgggtaaggg tatttatgac gtagtggctt
     4861 ttgaaaaaag tcttgaaaat aaaataccag aaaacaggct tctcagtcat gatttatttg
     4921 aaggcattca agggcgctgc gctcttgcaa gcgatattgt tctgtatgaa ggttatccgt
     4981 ctcattatgt tgcgtatatg aagcactctc atcgttggat tcgaggggat tggcagttag
     5041 ttccatggct aaaaaaaaga gtgcgaggac gagatgggaa aaagtataaa aatacaattt
     5101 ctcgaatcaa ccggtataaa atatttgata acttgcgacg cagtctcata cctttttttg
     5161 ttatgctctt gctttttgca agctggtttt atttgcctgg tccaaaaata ctttggattt
     5221 ttcttgcact tacaccctat ctaccgagca ttttctttag ttttatagat tcactttttg
     5281 ggccaaaaga aagtgatggc cctcgtggcg atgtaaaaat aaatcggcaa gcgtttttac
     5341 gaacattttt tgaattgagc tttttacctc acaaaacctt cgttagcctc gatgctatca
     5401 ttagaacgct ttggagactt tctttttcta aaaaacattt gctagaatgg gtttctgctg
     5461 cccaaaacga aaaaagatat ggcaaaaaag atatggcttc tattatttta aaagtgtttt
     5521 ttcccacaca agtgtttagc ctccttgttt tttttgtcgc tttctttttt gcacctacac
     5581 agatttttta tcttctcccc tttgttttgc tttggttttt tgcacctttt atagcaatac
     5641 gaataggtaa ggcgcaagaa cacaaaacag aaaaacttac agaaaatcaa gataggcttt
     5701 tgcgccgtct ttcacgaaat acctggctct tttttgaaca ttttgttggg ccagaagata
     5761 actggcttcc acctgatcat tatcaggaat cgccacgggg tatcgttgct catcgaactt
     5821 caccgacaaa tatagggctt ttgcttttat caagcctatc tgcttttgat tttgcctatt
     5881 ttggccacct tgaattgtat acgcgattac aaaactgttt tgacggcatg aagggcttag
     5941 agcgttacag agggcatttt cttaactggt atgatacaaa atctttgtcg ccattgaatc
     6001 cgcgctatgt ttcaagcgtt gacagtggga atttggccgc aagcattatg atcgctgcgc
     6061 aagcgccaaa agatgtttta caaaaaccag tcattcgtct tgcggggctt ttggacagcc
     6121 ttgatttgct ttttgatttt tctcgaagtc tttctcttcc aaaaaaaagc gaagatttag
     6181 aaaaaaacct tcttgctttg atgaaaaaca ttcaagccat tgatgaggaa gacagtgatt
     6241 ttcccgtgca gttaaaaaaa ttactcgtcg atgagcccaa gaaacttgag gaagggcttg
     6301 aaaagctcat acacaaatct cgtcaagaaa tagatacaaa tcttttgcaa agcctttcta
     6361 catgggtgga tcgttgtttg cgtcatatgc gaaatgtaca gcttgatata gagcgctata
     6421 ttccgtggac ttttgccttt gcagatattc ctgaagaatt ggaagcggtt ctttccaaca
     6481 atgaaatttc tgatctatgg ttgcgtcttg aaaaaacgct ggttttcaat cctccttttg
     6541 aagcaattcc aaaaataacc gtaaatgcgc ttattattct tgatgattta atgccacttg
     6601 ttaagacaga gatttttgta agttggtttg aaaatctacg agaaaaaata caagaaagcc
     6661 atattgcttc tagaagtctc atttcagatt ttagcgagtt ttcaaaaacg gctgatcttt
     6721 tggtgcgaga aatggatttt agctttttat tcaatacgca gcgaaaggtt tttcatattg
     6781 gctataatct tgattctgga caaatggatg aaaattatta cgacttgctt gcctctgaag
     6841 cgcgcattgc aagtattgtt gcgataggta agggtgatgt gccgagtagt cattggctcc
     6901 atatggcacg tcccctaaca aagcatgcag gaaaacagct tctcctttca tggagtgcaa
     6961 ctatgtttga atatctcatg cctctactat ttatgaaaac atatgaaaat tcccttcttt
     7021 cgcaaagttg tacgcttgcc gttgaaaaac aaattgccta tgggaaagaa aaaggaacgc
     7081 cctggggtat ttcggaatct agctactatc attttgatgc atcggaagtg tatcagtaca
     7141 gggcctttgg tgttcctggt cttggctata aacgggggct tggggaggat catgttattg
     7201 ccccctatgc aagtattttg gcgctcccaa ttgctccgaa ggctgttttg caaaacctta
     7261 tgcagctaaa aaagaaagag gcttgggggt tttacgggct tattgaatcc ctcgatttta
     7321 caaaacggcg tttacaacca gggcagaact ttgccttagt taaaacttat atggcgcatc
     7381 atcaaggcat gattttatta gcgctttcaa attattttaa caataataaa catatcgcac
     7441 ggtttcatgc tgatccgcga attaaaagcc ttgagttctt actacaggaa caaaatccga
     7501 aatctgcgcg tgttgaacat ccacatccgc aaagtcttgg gcttgtgcat cctattaaat
     7561 ctattatccc aatggaatcc tggcctatta tttcctctct tcgccagccg cagctgaata
     7621 tgcttaccaa cggaaattac actctcatga taagtgcttc tggggcaggt tttagtcggt
     7681 ggaagggtac agacctcaca cgatggcgtg gcgatgcgag tcttgacaat catggttcat
     7741 ggatatatct aaaagatttg gatagcaata aactctggtc tccaacagcc caacctatgg
     7801 gaggaaatat tgaaaagtct caggtttcgt tctatcctca taaggcggtc tttgaagaaa
     7861 acttaaatga agtgcataca aaaatggaag ttgcggtttt tcaagatgcg gatgctgagt
     7921 tgcggtatat ggagctaaca aatgcaaata atgaaagacg aaatctttcg cttacgagtt
     7981 atggcgaagt tatacttaca agccaagaag ctgacagtcg ccatcctgct tttaataaat
     8041 tatttataga aagtgaatac atagaagact ataatgcctt gcttataaaa agacggcctc
     8101 gctcaaagga cgaaaaaaca ctttatttta tacatcttgt tcaaggagaa aaagatgatt
     8161 tttcagtgca tagttatgaa acagatagaa gtgttttttt gggtcgtggt ggaagtattc
     8221 aaaatcctca tgcccttcaa gatgaagaaa aacttacaaa gactttgggt tttagtttag
     8281 atcccattta tgccttgcaa acgaagctaa cattaaagcc ctatgaaaca aagaaaattg
     8341 cttttataag tttagttgca gaaacgaaaa aagaagcgct tcgtcttgca agccgttttc
     8401 agcagtggta cggtatagaa aaatcaagag aaatgcttag cctctatgca gaagaagaga
     8461 tagagcgttt tgaactatcc atagaaaact accagttaaa tgcaaaaatg ctttctgctc
     8521 tgctttatcc ttcacaagca atgagagcaa atgctcagat tctcagaaaa aataaacttg
     8581 gacaagcagg tttatggccc tttgcgatat ctggtgatta tcctatcctc gtcttgaaac
     8641 tttcaaaaaa cgggacagaa ttattgcaag agcttttgaa atcgcattcc ttctggcgaa
     8701 ggcgcggtgt aaaggtagat tttgttatcc ttaatcaaag gatttctgtg tatgaacagg
     8761 aactttcaaa tagcatatat cgcttagtga atcaaagcgg caatgaaggt atgataaatc
     8821 agcgaggtgg tattttcatc cttcgtgaag atcagatgaa tgaggatgag aaactccttc
     8881 tctatgcttc tgctcgactg attttggaag gggaagaagg ttcacttgaa aaacaactcg
     8941 aaaaatttgc tttagaaaca atgcgtctgc ctcgatttgt tgcagttaaa ccaccctact
     9001 ggcttaaaga aggagggcat cttatttctc gccctgaaaa cttagccttt gacaatggtt
     9061 atggtggttt tagctcagat ggtagagcct attctatcta tcttgaggga tatgagcaaa
     9121 caccagctcc gtggataaat gtgatcgcca ataaagattt tggctttctt gtttctgagc
     9181 gtggtttggg aacaacctgg gcgctcaatt cgggcgaaaa taggcttacc acctggcata
     9241 atgacccagt tttagattct ccatccgagg ctctttattt acgagatgag gatacgggac
     9301 aaatctggag tccaactccg ggaccaaaac gagacggtgc accgtatctt ataagacaca
     9361 agcccggagc gagtatcttt gaacatagta gctatggcat agtgcaaaag ctaaggcttt
     9421 ttgccgatgt ggaaaaacct ataaaaatgg tccaactgca aataagcaat aaaaccgaag
     9481 agacgcgaag aattactgtt tcgtattatg cagagtgggt tttaggagat ttacgagaaa
     9541 aacatgcagc ctatataata cctgagtttg actcaaagca ttcagccctt ctcgtgcaaa
     9601 acccctataa tgtgcaattt gcatctcgag ttgctttttt agcctctact cgtgaattga
     9661 actgggttac cagtgatcga cttgagtttt tgggagcacg aaaaagctac gaggaacctt
     9721 ctgcattgag taggcttggt cttacgggaa atgtaagccc aggctttgat ccttgtgctg
     9781 tagtacaaaa ccttatttgg cttgctccag gtgaaacaaa ggaagtaaca tttttacttg
     9841 ggcagggaga agatagagac aatgccatcg agcttataca agagtttcaa gatattcaaa
     9901 atattgaaaa agcctatcgt agaagcataa aaaaatggga agaaatactt ggtacaattg
     9961 aagtaaagac tcccgaaaaa acgatggaca taatgctcaa tacctggctt ttatatcaat
    10021 cactgtcttg tagaatgtgg gggaggacag ccttttatca atcgagcggt gcctatggct
    10081 atcgagatca attgcaggat agtatggctt ttttacatag cagtcctgac atagcccgcg
    10141 aacatattct tcgatctgcc gcttcgcaat ttgaagaagg tgatgtgctt cattggtggc
    10201 atccacccac aacacgtggc attagaagcc gcattgttga caatctcttg tggttgcctt
    10261 ttgtgaccgc ccgctatatt tctgtaacag gagacatgtc tatattggat gaagaaattc
    10321 cttttttaca tgcaagtctt ttagaagaga atgagcatga gcgttttaat gtctttgccc
    10381 atggcgatga gtcggcctca ctttatgagc actgtttacg agcgatacga aagggtgcaa
    10441 caaaaagcgc aaaagggctt cccctcatgg gtggaggaga ttggaacgac ggcatgaatc
    10501 gcgttggaaa agatggcaag ggtgaaagca tttggatggg cttttttctc atcgcttgct
    10561 tgaagaattt tgcagaagtt tgtcttgaaa aaaatgatag tgaagaagct gagaagttta
    10621 aatctatggc aaaagagtat acgaaggcta tagaaacaca tgcctgggat ggtgcttggt
    10681 atagaagagc gtattttgat aacggcgacc cactcggttc tagcgaaaat gaagaatgcc
    10741 gcattgattc tataagccaa tcttgggcta ttatttctgg agctgcagaa aaagaacggg
    10801 cagaaatggc aatggcttcc cttgataaag aactcgttct caacgaagag aaaatacttc
    10861 ttttactccg ccctcccttc aataatagca aaaatgatcc aggctatatc atggcctatc
    10921 ctcctggaat tcgagaaaat ggtggacaat atacacacgg tgttttatgg gcactttggg
    10981 cacttgcaaa aatcggcaag ggggatagag cctttgaatt attcaatatg ataaatccta
    11041 taaaccatgc aagacaaaag gcagatgcaa aactctatcg tgtagaaccc tatgtgcttt
    11101 cagcagatgt atatggcgcc catccacacc ttggacgagg aggctggaca tggtataccg
    11161 gctctgctgc ttggatgtat caaataggaa tagaacagct tttaggtata caaagagaaa
    11221 gggacgaact tattatcgag ccttctatac caagacactg gccaggtttt gaaattaagt
    11281 atcgttttgg gaagagccta tacaaaatag aggttgtaca aaagagcaat acgggcaaaa
    11341 ctgtaaaaga agaagagcga agtctaagcc ttgatggaaa agaactagaa ggcaacagcg
    11401 tagcccttgt tgacgataaa aaagaacata gaataaaact aataataaaa acagtagata
    11461 ggtagcgcct tgggctttgc ctcgctatgt tttttagcag gcacttattt tagtgttttt
    11521 tatttatcta tctggtatta aaaagaatgt acattatgcc gataatacat tatactgttt
    11581 agagtgttta gtgataaaag agaaaggggt tttcatgaac aatgttctat tatttaagaa
    11641 aaaaattgta tgtatagtgc tttttctttc tgtgtatttt tttaatggtt ttgcacaaga
    11701 cactttttta ctttatatgt attctcaagt aaatgatcac gttgaaataa aaaatgtttc
    11761 ggcgataaat cgtattctaa taaataacaa gcattcgcgc gattatgata aattggagaa
    11821 ttatatcctt atgaagtccc gtgagcagct tgtgatgaat aatttagatt tagcaatgga
    11881 tttatgctat attgtcattg acaataattt agataacttt gaagctgttt ccttatatac
    11941 atctatagaa cgaacaaagc gagaaaaaga agaaagagag cgtatagaaa aagaaaaagc
    12001 tcagatagaa tcgataaaac aagctgaaac aataaaaaaa gataccgaaa aaatacagag
    12061 agagtaccag accataacaa atacagctag tgggcaacgt gtatatttag accaagatgt
    12121 agatgattat tttcaaacgg taacatgggg cttggggctt ggtttagcaa gtttttcagc
    12181 tattatcgat attccacaag ttgaaggttt ttatggcatt tcagtgcttg gcaatgtatt
    12241 ctatagaagc gaagtaattc atgcgggtat cgatgttttt gctgctatgg cgctgtcttc
    12301 tttttctgat ttagattttt tacctgccaa agcagaagcg aatctaaatt tcgcctttcc
    12361 aaaattggca aagtatttgt attaccgtgt cggcgttgtt gggtttttat cttatctcgg
    12421 agaagaaagc tttttgccaa aacaattttt aacaccgact gctggaatcg ctttaaaaga
    12481 tattggcaca gaagcctttc tttttggttt gaatgttgac tatcatttag cccatcttta
    12541 taatgcggaa atcggaccag gtctttctgc cgggctaaac ttttttattc gttttgccga
    12601 tttaaaaaaa gcggatgttg gtttgattat tggtctcgag gatagttttt tttatacgtc
    12661 taatggcttt caaaaccatg caaaaataaa tctaagtatt ggagtttgga ataatgatta
    12721 aaaaaagtgc ttttattgtt attttctttt gttctttttt ctttcttaat gcacaaagct
    12781 cccttgtaga gcgatattta gctgcagcca acacgagcta ttccgaaaag gactatgcga
    12841 aggcttttag ctatataaat tatgtatacg gtcagtatga tgcggacaag actccgcgaa
    12901 atgttgaagt gctcgctgaa cttatctact atgattatct aaatcacatg cgggttacga
    12961 acaatttttc tgagttccag gtagttaaag ataggctcgc tgaattccct gctatttcat
    13021 cagatagggt gactcgcctt gttcgtacct taaatacgct cgaatctcaa aatacaaatt
    13081 tggaaacgac tgtgtcaacc gtacttgatt tacgtgatgc ggttgaatat aagatacaat
    13141 tagaaagaac gaaggctgag ttgcatgcat tggaaaaagc tttggaggat gcacgagaaa
    13201 gtgctgaaaa agaaaaatta tacagagata aagagtttct tgaaacacaa aaagaattgt
    13261 atgagaaggc attaaatcta agtaatgagc gagatggagt caataaaacc tttgttttat
    13321 tgattataat tgctgttgtt gtgcttttct ttgtattgtt tgttgtgatg atgattatcg
    13381 tccgtatttc aacaaaaaac gctaaaaaac aacaggagca gtttgaggca accttgcaaa
    13441 tggtttctca aatagctcga aatccgagtg aacgtttgag cttgggcagt tttgttgact
    13501 cctttggaga tgctgcttca aacggtcgca ttgggggaac acaggcttct ttgccacgtc
    13561 cggaacttac tgaagaggag cggcttgagt tgcaagacct tgcattaaag tgcgaggcag
    13621 taggtgaaga tatagatcgt gtaacgggac gaaaaaacaa ttcgaaaaat gtagcagagt
    13681 tggtatataa aatatctcat cagctttcct gttcaaccta tgactctatg ctttattttt
    13741 gtgcggcaat ggtttacgat gccggctttt tgaaaataga cactgaaatc tttacaaaag
    13801 aaagcctaag caccgaagaa aaatacgaaa tacgaagcca cgtgaaacaa gcaacagaaa
    13861 gctttgactt tataccagaa aaatatctgc cagtattttt agatgctgct ctcatgcatc
    13921 acgaaaacat ggatggtagt ggctatccta ctggggcacg tggcgaaaat attccgctta
    13981 tcgcacggat tattcgtgtg gtagaaagct ttatatcgct tatttctcgt agaaactacc
    14041 gaggcatttt tgacaaagaa gcagctatta ccgaattgaa aaatagcagc catttatatg
    14101 actctgcgat catagatgcc cttgatacga taatataagc ggttgtgttt tttatactag
    14161 aataaaatcc tttacaaaac tcattattaa acgtataatt atggtgggta tttgtataaa
    14221 tgaaaggatt atggtgtgaa aagtaaaaat gtaaaacaaa aatataaaat cattttactc
    14281 aatctctttt tactgagtgt ttttagttgt acaatgaatg gtgaaaatcc ttttagcact
    14341 aaaaaatacc ttagtataca ataccctcct gaaaatgcac ttatacgtaa ttcatttact
    14401 atgaatggac gtattctaac caataaaaag cttaaatcaa tcga
//
LOCUS       DAOSSW010000046        13943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_4462_length_13943_cov_6.569196, whole genome shotgun sequence.
ACCESSION   DAOSSW010000046 DAOSSW010000000
VERSION     DAOSSW010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 13943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13943
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4462_length_13943_cov_6.569196"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..1266
                     /locus_tag="PKW26_05185"
     CDS             22..1266
                     /locus_tag="PKW26_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOQ92992.1"
                     /translation="MVFIIILLLFFIAFFSFAETALLSLTKVQLKQLLLAEKKKGKRI
                     ADLKNSMDQVISIILIGSSFLNTLASALATVYAIRLVGAQGAVWATLVMLILIILFGE
                     IIPKNIAALYPKEASLLSAPILTVLLFVFKPIVFLFSLLQKGINKIERSLWKNKEPII
                     TEEELKTLIALGDKEGTLERGEKDMLYNIFELNDLRAKDIMKHRSLIKTCNINASLHQ
                     SIEAFEKSGYSRLVVIEDSIDAINAIQGILHFKDLLFFDSAQDFSVKSIMRKPLFIPE
                     SKSVISLLGAFKKEKQNFAVLVDEYGSNQGIITMDDILKAVFGRITDEHNTEGKNAEE
                     RIQIIKTNQFLIPGELSIDDFNRIFKQNFSSEAFESIGGFLLEEFGYLPQAGEFLKRN
                     NCILAVDAQSQRRIKMIKLTIL"
     gene            complement(1272..>1814)
                     /locus_tag="PKW26_05190"
     CDS             complement(1272..>1814)
                     /locus_tag="PKW26_05190"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HOQ92993.1"
                     /translation="LSFEKNSFGLGTSFEWRSIQLTSTGGIQYMYLHGDETDANAEKI
                     ALSGKGITYKQNIFLWWLNASFSLGLTKKLTADFIFSYSPFWYGEGYDRHHHRSLDFL
                     DKVEGHTAYKLASHFMLQIREPVVLGLSINYLSIPMKVGSSASKKTWETQYRAFDSSL
                     GGMSLSEFSAAVFLRYRFSF"
     assembly_gap    1815..1914
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1915..2234)
                     /locus_tag="PKW26_05195"
     CDS             complement(<1915..2234)
                     /locus_tag="PKW26_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ92994.1"
                     /translation="MKKTALFFCFFLLFFLRLSHQAYSLDFYLSPIFEYRNARLGEYV
                     VIPENFSYTGKNWILSDLQWQANTIFNLGAEAGLDFRNISFFTKALFGLPGEVGSMTD
                     EDFI"
     gene            complement(2249..2995)
                     /locus_tag="PKW26_05200"
     CDS             complement(2249..2995)
                     /locus_tag="PKW26_05200"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOQ92995.1"
                     /translation="MFKTQNFSVIYSDESLLAVSKPSGLLVAQDRYDVDAPRLDLLLS
                     KEFGKLWAVHRIDKDTSGLVIYARTSEAHRALSMQFQERKVKKSYLALVYGRPSWEEK
                     KVSTPLLVDGDARHRTIAHKQLGKESLTEFRMKGSCGPFSWIEARPLTGRTHQIRAHL
                     LDLGFSIVCDPLYGGNQKPFFLSDIKRSYKGDLFEERPLLSRLALHAFSLEIIDPTTN
                     EKRYFEAPLPKDMDVVRKQCAKLFKVDPLG"
     gene            3100..4434
                     /locus_tag="PKW26_05205"
     CDS             3100..4434
                     /locus_tag="PKW26_05205"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOQ92996.1"
                     /translation="MKGIFDFTVDTLGQCKNPSPIKLSTVEGDFVANYVKDNEFIRYN
                     VDVYAGEKDATETYLSNLIEKAGPRELIYFNPAHVNAGIVTCGGLCPGINDVIRALVR
                     CLYNRYGVKRVRGIRFGFKGLLPEEGFSPIDLTPDIVDDIHKVGGSFLGTSRGGGDRV
                     TEIVDALESLNMNMFFVIGGDGTQRGALEIAEEIEKRKLKIAVIGIPKTVDNDLVFIQ
                     RSFGFDTAVQQATEAVSAAHMEAHSHINGIGLIKLMGRESGFIATTTALASHEANFCL
                     IPEVPFDLEGEQGFLMELEKRLLRRQHAVVIAAEGAGQELLNATGEKDASGNIKLGDI
                     GTFLKDTITAYFKKRKIHINLKYIDPSYQIRSAASTPTDSVYCERLGNNAVHAAMAGK
                     TKIVIGLVHNKFVHLPTRLVTAERNRVNPDGSLWRDAMDATGQPALMVNPKK"
     gene            complement(4496..5908)
                     /locus_tag="PKW26_05210"
     CDS             complement(4496..5908)
                     /locus_tag="PKW26_05210"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ92997.1"
                     /translation="MGIKLYSLGAAREITGSKHVLEVDGRSFLIDCGAFQGKRKEADE
                     KNRNFDIVGEDLEAVILTHAHYDHCGLLPVLQKKGYTGNIYSTAATRDLANLIMMDSA
                     HIQARDREYLQKQAAKKGEKFTWKPLFNEIDVVATTNQFVSISYNRKMFIAPDMELEF
                     FDAGHILGSSFANLSIRRDREDINVLFTGDMGRKNKPIIRDPEVNVGAPDYIVIESTY
                     GDRLHDDVEVAMDELARVVQRVVKQKGKIIIPAFAIERTQELVFYFHLLVDAKKIPEI
                     PIYVDSPMATNATSIFQVHPECYDKSVHEAFLKHHKNPFGFNALSFVTSVAESKSLND
                     KKGPMIIISADGMCEAGRIVHHLANNISNPNNTVLLVGFMAEHTLGRRLQNREKEVRI
                     MGDWYEVKADIEQINAFSGHADYKEMIDWLKSIDTSKLKKIFMVHGEIDAQEFFKKEL
                     IKNGFPDIEIVAYDKKYTLN"
     gene            6018..>6683
                     /locus_tag="PKW26_05215"
     CDS             6018..>6683
                     /locus_tag="PKW26_05215"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HOQ92998.1"
                     /translation="MYNRKIKEMPNSLISNGKATFGTFMGHPKKLDIKDILFPFGVLP
                     LPRFITNLRIRSSIFFDFSTDAFIGKIELFDTKIIGYYKLIVWEKITHKKYSYHHLMF
                     LNRRLIPTNLEKASCTVFSKKRYARFTWDRTKDIFSLVFKCKGDSHRPSISASLQASF
                     SNPQFLETTNVSPAPTTRRCSALFQMTNSFNATFSIRQKEYHEQIITQNGLFSFGLKR
                     AYYN"
     assembly_gap    6684..6783
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6784..7237
                     /locus_tag="PKW26_05220"
     CDS             <6784..7237
                     /locus_tag="PKW26_05220"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HOQ92999.1"
                     /translation="IITQNGLFSFGLKRAYYNVRYYEENISFQLQNEDGAVFLNLFSS
                     SIDSHNDDLHNSNILIENADLSLLPPVKITRPQGFFNEWIIQDTESMVDLSFKPIVND
                     LRRLSIFLLHARFHTLLGTLDGSVRTKDNKEIQLKNIHAIASKQRLRL"
     gene            7253..>8534
                     /locus_tag="PKW26_05225"
     CDS             7253..>8534
                     /locus_tag="PKW26_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93000.1"
                     /translation="MSKAKTEKPKAVYEPGELDKTRKNIGEISPEEAMAIAKKLGGKI
                     GVEKTQDFSTALYKSTKRYVKRGDKASPILQKSTTTNQTNEKKESVSQAHFKKDKNHG
                     QNILPIMHSQSKKLLDTLMSSQEYRIKPNYGIFTTIVNALQGNQEKVAPQFILYNLQH
                     YFLSLQKFHIALNNMLNYTADDFKRRLANPKTNYYKAIDFISKWDIKELQEYYQVLEK
                     NPKAVTIVKLIPFTKILFRFILPIYYLGEETIISYIKQIYTDNADFLKTSKEAFINQT
                     KEAASQWIYIYGQIAKGMYPILLRMTSNDFVEYPAFYLKRTTKILQFLNLSKFDILFP
                     ENKVTKSKPKIKIEEENKETETEKEKKEFEKNETNEEKETDSLLIAKGLDILDTLFPD
                     AGWKDIGSAPDLYPYFQPIYNFADGFNLLAPSNPM"
     assembly_gap    8535..8634
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8635..9483
                     /locus_tag="PKW26_05230"
     CDS             <8635..9483
                     /locus_tag="PKW26_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93001.1"
                     /translation="PIYNFADGFNLLAPSNPMQVTIILHRILEDFFQALRNIRYSIDK
                     EPEFTVFHDDINAIFSEWSTYREYQFERIYLPELKEYVNQAYTQPEFVKTPYARKLMS
                     NFFWQTKHHFLPHISFELIFMEKPSKDTSHIPFPNRVHFLKKIYKTLVNRVEQNLSAP
                     VKEGNKNDDIYGAQGLYLPYRFDIPNPVSKRVDVLLNKKKGKNANNLNLIKYTVCILA
                     VLDWWINSKESPANKETVKTPYRLSPEDGTPVFYVTERKDLDKVFIQNVKAKLLHKEA
                     QEKEKE"
     gene            complement(9489..10325)
                     /gene="thyX"
                     /locus_tag="PKW26_05235"
     CDS             complement(9489..10325)
                     /gene="thyX"
                     /locus_tag="PKW26_05235"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOQ93002.1"
                     /translation="MARCIVPEAEAILDKEYKVLDKGFVRLVDYLGGDARIVQAARVS
                     YGEGTKTVREDAGLIDYLLRNDHTSPFEQVVFTFHIKMPIFVARQWLRHRTARLNEIS
                     GRYSVMKDDFYIPASEDIALQNKDNKQGRASEESDADFAKQVQESLGEGQKNSYAEYS
                     KLIDTGLAREIARINLPLSLYTEMYWQIDLHNLFHFLRLRLDAHAQKEIRDYAKVLFE
                     IAKKVSPFACASFENYKLGGVNFSKAEFEELKLILENKNGKLEGKEKERFDKKMQLGR
                     QV"
     gene            complement(10348..11610)
                     /locus_tag="PKW26_05240"
     CDS             complement(10348..11610)
                     /locus_tag="PKW26_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOQ93003.1"
                     /translation="MKIETQCIHEGYSPKNGEPRVMPIVQSTTYRFDSTEHIASLFDM
                     PTEFMYSRFANPTCDAVEKKLAALEGGLAAMLTSSGQAANLFAILNLCNAGDSFIAAT
                     GIYGGTVNLFAVTLKKFGIECIWVSPDASDEELEAAFKENTRAVFGETLTNPALTVLD
                     IERFAKFAHKNKVPLIVDNTFPTPILCKPIDFGADIVTHSTSKYLDGHALQCGGVIID
                     SGKFDWKNGNFPEFTEPDESYHGIVYTEAFGSLAYIIKARMQLMRDFGSYPSAHSAFL
                     LNIGLETLHLRMKEYCVNAMQVAEFLAKSDKIEKVRYPGLKNDRYYALAQKYLSDGSS
                     GVINFTIKGGREAAVRFMDSLSLASNVVHVADIRTCVLHPASATHRQLTDEQLSAAGI
                     DAGMIRFSCGLEHVDDIIADITQALEKV"
     gene            complement(11641..12765)
                     /locus_tag="PKW26_05245"
     CDS             complement(11641..12765)
                     /locus_tag="PKW26_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93004.1"
                     /translation="MKKRSPFYLVLVLFLFSCASIQRDLYIASPQTELGQELLFLESE
                     LISLDALDIEAAHAGHTGYRLSDEAYTSLLQKIEAFLVQEPDQNSKARLYALKGRLHL
                     LYAQGQTAKSQKKLAQKALQDAEKQNPYDLQVYVLKKRLGEIESFSKELLDRDSSNVL
                     RIEQALSFFQNSLYAEAVSSFDSAFRLAEPMYQSVYGNIRNRAWALREVKESTSKDLD
                     SYLKKNSLTLQELVQVIDDNSSAFECITGGKKYKPSDLYLHLQNLDLFSPLSEVGIRE
                     QEKIKANDEATRRYSARLLWRLYLINTSKQDQKNIYSQFYRKKTDAKSPILDVFLDDA
                     DFDAILACVEREWMDLPDGKHFYPEKGVEFLELLEWIKNF"
     gene            complement(12765..>13943)
                     /locus_tag="PKW26_05250"
     CDS             complement(12765..>13943)
                     /locus_tag="PKW26_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93005.1"
                     /translation="YDLEKTLPFFAQGDSILAQVLQLYSVGRGEVQVSDQRLVTSLHA
                     KSGAKTKSRAIHGFPIDLDGKASSNLVLIEKSKNPTLFWVEKNRTLFALDLKTLQKKN
                     FEFSENIHVLAADAEASFLWVLSTSGTVYKINSELETEAGFPVLTGEKIAAPGISLGD
                     SFLFPTEKNTVVFVSAEGTLEEKTLDTMGNFVSSPAKTDRLAAFYAKSFLGDIFVFDE
                     ESCLNAENPLRVESIGFGSPAILEKSRSVYIAFIGQAGDLYIWKDFSLLEGFPRQIEA
                     VFRENLIAFADAFFAIDESGIVYKIGLDGRLLAQRDIGFTAKNANLSVHQYRDKKNAL
                     FINPDASLLFAFDENLDELSFSPLSGMYVPVFVDLNGDKKNDMLVLSADNKINAWMVD
                     "
BASE COUNT         4469 a         2824 c         2375 g         3975 t
ORIGIN      
        1 aggtagggac ttaggagaaa aatggttttt atcattatac tattactttt ttttattgcc
       61 tttttttctt ttgctgaaac agcgctttta tcgctcacaa aggtgcaact aaagcagctt
      121 ttacttgcag aaaaaaagaa gggtaagcgt atcgcagact taaaaaattc tatggaccaa
      181 gtaataagta ttattttaat cggttcgagt tttttaaata cccttgcttc agcccttgcg
      241 acagtttacg cgatacgcct tgtgggagcc cagggagccg tatgggcaac cttggttatg
      301 cttattttga ttatactttt tggcgaaatt attcccaaaa atattgcagc actttacccc
      361 aaggaagcaa gcctcctgtc cgcccctatt cttactgttc tcctttttgt ctttaaaccc
      421 attgtttttc ttttttcact tttacaaaaa ggtataaata aaatcgagcg ttctttgtgg
      481 aaaaataaag aaccgattat tacagaagaa gagctaaaaa ccctcatcgc tttgggagat
      541 aaagaaggta ccctagaacg aggcgaaaaa gatatgcttt acaatatctt tgaactcaat
      601 gacctgcgcg caaaagatat aatgaaacat cgctctctta taaaaacatg caatataaat
      661 gcgagtttac accaaagcat cgaagccttt gaaaaatctg gatattcccg tttagttgtt
      721 atagaagatt cgatcgacgc tataaatgcg attcaaggta ttttgcattt taaagacctg
      781 ctttttttcg attccgcaca agatttttca gtaaaatcca taatgcgaaa acctcttttt
      841 attccagaat caaaatccgt catttcctta cttggcgcat ttaaaaaaga aaagcaaaac
      901 tttgcagtcc ttgtcgatga atatggaagc aatcaaggta ttatcaccat ggacgatatt
      961 ctcaaagccg tttttgggcg catcacagac gagcacaaca ctgaagggaa aaatgccgaa
     1021 gagcgcattc aaattataaa aaccaatcag tttttaattc caggggaact ttctatagat
     1081 gattttaatc gtatctttaa gcagaacttt tcttctgaag catttgaaag catcggcggt
     1141 tttcttttag aagaatttgg ctatcttccg caagctggcg aatttctcaa gcgaaacaat
     1201 tgcattcttg ctgtcgatgc acaaagtcaa cgccgcataa aaatgataaa gcttactatt
     1261 ttataatcta actaaaaaga gaaacgatag cgcaaaaaaa ctgctgcaga aaattctgaa
     1321 agagacatgc caccaagact agaatcaaat gctctatatt gtgtttccca tgtttttttt
     1381 gatgccgaag atcctacttt catgggaata gaaaggtagt ttatactaag tcccaaaact
     1441 acaggttctc gaatttgcag cataaaatgt gaggcaagtt tatacgcagt atgcccctca
     1501 actttgtcta aaaaatcaag agaccgatga tgatgtctat catagccctc tccataccaa
     1561 aatggactgt aggaaaaaat aaaatccgca gtcaattttt ttgtcaaacc caatgaaaaa
     1621 gatgcattca accaccacaa gaaaatattt tgtttataag taattccctt gccagacaaa
     1681 gctattttct cggcgttcgc atctgtctca tcaccatgga ggtacatata ctggattcct
     1741 cccgtagatg tcaattgtat ggaacgccac tcaaaacttg ttcctaaacc aaagctattt
     1801 ttttcaaaag aaagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaaata
     1921 aagtcttcat ctgtcataga accgacttct ccaggaagcc caaacaaagc ctttgtaaaa
     1981 aaagatatat ttctaaaatc cagtccagct tctgccccaa gattaaaaat agtgtttgcc
     2041 tgccattgca aatcacttaa aatccagttt ttccctgtgt aggaaaaatt ctcagggatt
     2101 acaacatact caccaagacg agcattcctg tattcaaata ttgggcttag ataaaaatcg
     2161 agactatagg cttgatgaga aagtctgaga aaaaataaaa gaaaaaaaca aaaaaacaaa
     2221 gctgtctttt tcattgtcat cctttttttt atccgagtgg atctacttta aataactttg
     2281 cacattgttt tcgcacaaca tccatgtctt tcggaagcgg agcttcgaag taacgttttt
     2341 catttgttgt cggatctatg atttctaaag aaaaagcatg aagggcaagc cgagagagca
     2401 agggacgctc ttcaaaaagg tctcccttgt aagagcgttt tatatcagac aagaaaaaag
     2461 gtttttgatt tccgccatac aaaggatcac aaacaatact aaaacctaaa tccaacaaat
     2521 gtgctcgaat ttgatgcgta cgtcccgtta gaggacgagc ttctatccag gaaaaaggac
     2581 cacaagaacc tttcatacga aactcggtca gagattcttt tccaagctgt ttgtgagcaa
     2641 ttgttcgatg tcttgcatct ccatcgacca aaagcggcgt ggaaactttt ttttcttccc
     2701 aagaaggacg accatagaca agggcaaggt agcttttctt cacttttcgc tcttgaaatt
     2761 gcatcgataa ggctcggtga gcttcgcttg ttcgcgcata aatcacaaga ccagaagtat
     2821 ccttatctat acggtgcaca gcccaaagtt ttccgaactc cttacttaaa agcaaatcaa
     2881 gcctcggtgc atcgacatca tatcgatctt gcgcaacaag tagaccgctt ggctttgaaa
     2941 cagcaagtag agattcatca ctgtagatta cggaaaagtt ttgtgtttta aacatttgta
     3001 gagtatagca caaaaaaaga atttgcgtaa agcttgtttt aatccttcat atttagtaaa
     3061 attcatatac taggcaaatt ctaaagacag gagaaatgaa tgaaaggcat ttttgatttt
     3121 accgttgaca ctttgggaca atgtaaaaac ccatccccta taaaattatc aacagtggag
     3181 ggcgattttg tagccaatta tgtaaaagac aacgagttca ttcgttacaa tgttgacgtt
     3241 tacgccggcg aaaaagatgc tacagaaaca tatctgtcaa atcttatcga aaaagcaggt
     3301 cctcgcgaac tcatctattt taatccagcc catgtcaatg caggcattgt aacttgtggt
     3361 gggctttgtc ccggtataaa tgacgtaatt cgcgccctcg ttcgctgtct gtataatcga
     3421 tatggcgtaa aacgtgtccg aggtattcgc tttggtttta aaggcctcct ccctgaagag
     3481 ggtttttctc ccatagacct tacgccagat atagtcgatg atatccacaa ggttggtggc
     3541 tccttcctcg gtacaagtcg cggaggtggg gatagagtta cagaaatagt cgatgcgctt
     3601 gaatccctca acatgaatat gttttttgtt atcggtggag atggcacgca aagaggcgcc
     3661 ttagaaattg ctgaagaaat agaaaaacga aaattgaaaa ttgcggttat cggtatacca
     3721 aaaacagttg ataacgacct tgtctttatc cagcgctctt ttggctttga caccgcggta
     3781 caacaagcaa cagaggctgt ttccgcagcc catatggaag cgcattccca tatcaacggc
     3841 attggactta taaagctcat gggacgagaa tcgggtttta tcgcaacaac aaccgccctt
     3901 gcaagccatg aggcaaactt ctgtctcata ccagaggtcc ccttcgatct tgaaggagaa
     3961 cagggctttt tgatggagct tgaaaaacga ctcttgcgtc gacaacacgc tgttgtaata
     4021 gctgccgaag gagctggaca agaattgctc aatgcaacgg gcgaaaagga tgcttcaggc
     4081 aatataaagc ttggcgacat cggcaccttc ttaaaagata caataaccgc ttactttaaa
     4141 aaacgaaaga ttcatattaa cttaaaatac attgacccaa gttatcaaat tcgatctgcg
     4201 gcctcaacac caacagactc tgtatactgt gagcgattgg gaaataacgc ggttcatgct
     4261 gcaatggctg ggaaaacaaa aatcgtcata gggcttgtac acaataagtt tgttcaccta
     4321 cctactcgcc tcgtaaccgc tgaacgaaac cgagtaaacc cagacggctc tctgtggcga
     4381 gacgcgatgg atgcaacagg tcagccagcc cttatggtaa atcccaaaaa ataagctaaa
     4441 aaaagctatc gcttacaaaa caatacaaaa acttcggcaa gagccgaagt tttttttagt
     4501 ttagtgtata ttttttgtca taggcgacta tttcaatatc tggaaagccg tttttaatca
     4561 gctctttttt aaagaactct tgcgcatcaa tttcaccgtg taccataaaa atctttttaa
     4621 gctttgaggt atcaattgat ttaagccagt ctatcatctc tttgtaatcg gcatgaccgc
     4681 tgaatgcatt tatttgttca atatctgcct tcacctcgta ccaatcgccc attatgcgca
     4741 cttctttttc acggttttgt aaacgccttc cgagggtatg ctcagccata aagccaacaa
     4801 gaagaaccgt attgtttggg tttgaaatat tatttgccaa gtgatgcacg attcgccccg
     4861 cttcacacat accatctgcc gagataatta tcataggacc ttttttatcg tttaatgatt
     4921 ttgattctgc aacactggta acaaaagaaa gcgcattaaa gccaaagggg tttttatgat
     4981 gcttaagaaa agcctcgtga accgatttat cataacattc tggatgtact tgaaagatac
     5041 tcgttgcatt tgttgccata ggcgagtcaa cgtaaatagg tatttcggga attttttttg
     5101 catcaacgag taagtgaaaa taaaaaacaa gctcttgtgt tctttctatt gcaaaagcag
     5161 gaataattat ttttcctttt tgcttcacaa ctctttgcac aactcgagca agctcatcca
     5221 tcgccacttc gacatcatca tgcaatctat cgccataggt actttcaatt acaatataat
     5281 caggagctcc cacattcact tctgggtctc gtataattgg cttgttttta cgtcccatat
     5341 ctcccgtgaa taaaacattt atatcttccc tatcgcgacg aatagaaaga tttgcaaaag
     5401 atgagcctaa aatatgcccc gcatcgaaaa actcaagctc catatctggc gctataaaca
     5461 tttttctatt atacgaaatt gaaacaaact gatttgttgt tgcaacaaca tctatttcat
     5521 taaaaagagg cttccaagta aacttttccc cctttttcgc tgcttgtttc tgtaaatact
     5581 ctctatctct tgcttgaata tgcgcagagt ccatcattat gaggtttgct aaatcacgag
     5641 tcgctgccgt cgaataaata tttcccgtat agcccttttt ctgtaaaaca ggtaacaaac
     5701 cacaatgatc atagtgtgca tgcgtcaaaa taacagcttc gaggtcttcc cctacaatat
     5761 cgaaatttcg gtttttttca tcggcttctt ttcgtttccc ctgaaaagcc ccacaatcaa
     5821 ttaaaaaaga cctgccgtca acttctaaaa catgtttaga accggttatt tcacgcgccg
     5881 cacccaaaga atacaatttt atgcccattt tgcctccata agctttcatt ttactatgct
     5941 tcctccaaat tcgcaagttt ttaaaattat tacttacaaa attcttttta tcgtatataa
     6001 taaaacagag acatagtatg tataatagaa aaataaaaga aatgcccaac tctcttatct
     6061 caaacggaaa agctactttt ggtacattca tgggacaccc gaaaaaactt gatataaaag
     6121 atattttatt tccctttggc gttttgccac ttccacgctt tatcaccaat ttacgcatac
     6181 gaagtagcat tttttttgat tttagcacag atgcctttat tggaaaaatt gaattgtttg
     6241 acaccaaaat tatcggatat tataaactca tcgtttggga aaaaataaca cataaaaagt
     6301 actcctatca ccatctcatg tttttaaatc ggcgccttat accaacaaat cttgaaaaag
     6361 cttcatgcac cgttttttca aaaaaacgct acgcacgctt cacatgggac agaacaaagg
     6421 atattttttc ccttgtcttt aagtgcaagg gagattctca tagaccaagt atttcagctt
     6481 cattacaagc atctttttca aatccgcaat ttttggaaac aacaaatgta agtcctgccc
     6541 cgacaacacg caggtgttca gctctttttc aaatgacaaa ctcttttaat gctacttttt
     6601 caatcaggca aaaagaatat cacgaacaaa taatcacaca aaatgggctt ttttcatttg
     6661 gcttaaaacg agcctactac aatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnaataatc acacaaaatg ggcttttttc atttggctta aaacgagcct actacaatgt
     6841 acgatactac gaagaaaata tcagttttca attacaaaat gaagatgggg ctgtttttct
     6901 aaaccttttc agcagctcca ttgactctca taatgatgac ctacacaaca gcaatatatt
     6961 gattgaaaac gcagatctca gccttctgcc tccagtaaaa ataacgagac cacaaggctt
     7021 tttcaacgag tggattattc aagacacgga aagcatggtt gacctctctt ttaagcccat
     7081 tgtaaatgat ctacggcgtt taagtatttt tctcttgcat gcacgttttc atacacttct
     7141 aggtacactg gacggtagcg tgcgcacaaa agataataag gaaattcaat taaaaaatat
     7201 tcatgcaatt gcaagcaagc aaagattacg tttgtaatgg ggtatgtgac gaatgagtaa
     7261 agctaaaaca gaaaagccga aagcagtgta tgagcctggc gagttagaca aaacaagaaa
     7321 aaacattggt gagataagtc ctgaagaagc gatggcgata gctaaaaaac tagggggaaa
     7381 aataggtgtt gaaaaaacac aggatttttc aacagcctta tataaaagca caaaaagata
     7441 tgtaaaaaga ggtgacaaag cttcacccat tcttcaaaaa tcaacaacga ccaatcaaac
     7501 aaatgaaaaa aaagagagtg tttcgcaggc ccattttaaa aaagataaaa accatgggca
     7561 aaacatactt cccatcatgc atagccaatc gaaaaagtta cttgatactc ttatgtcaag
     7621 tcaagaatat cgaataaaac ccaattacgg catttttacc accattgtca atgcactcca
     7681 agggaatcaa gaaaaagtcg cgccacaatt tatcttatat aatctacaac attatttttt
     7741 aagtttacaa aaatttcata tagctctaaa taatatgctc aattatacag ccgacgattt
     7801 taagcgccgt ttggcaaatc cgaaaacaaa ttattataaa gcgatagact ttatctcaaa
     7861 atgggatata aaagaactac aagaatacta tcaagtgctt gagaaaaacc ccaaggcggt
     7921 aacgattgta aaacttattc catttaccaa aatcttattt agattcattt tacctattta
     7981 ttatcttggt gaagaaacca tcatttcata cataaaacaa atctacaccg ataatgcaga
     8041 ttttttaaaa acctcaaaag aagcctttat aaaccaaaca aaagaggccg catcacagtg
     8101 gatctatatt tatgggcaaa tagcaaaagg tatgtatcct attcttttgc gtatgacaag
     8161 caatgatttt gttgaatatc ctgccttcta tctaaagcga actacaaaaa ttttacaatt
     8221 tttaaatctt tcaaaatttg acatcttatt tcctgaaaat aaagtaacaa aatcgaagcc
     8281 taaaataaaa atagaagaag aaaataaaga aacagaaaca gagaaagaaa aaaaagaatt
     8341 tgaaaagaat gaaacaaacg aagaaaagga aacggactct ttgctcattg caaaaggctt
     8401 agatatctta gataccttgt tccctgatgc gggctggaaa gatattggat cagcgccaga
     8461 cctttatccc tacttccagc ctatttataa ttttgcagat ggatttaact tactcgcacc
     8521 ttcaaacccc atgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncctatt
     8641 tataattttg cagatggatt taacttactc gcaccttcaa accccatgca agtaaccatt
     8701 atcctacatc gtattttaga agattttttc caggcgctta gaaatatacg atacagtatc
     8761 gataaagaac cagaatttac cgtatttcac gatgatataa atgctatttt ttcagagtgg
     8821 tcaacatata gagaatatca attcgaacga atctatctcc cagaactaaa agagtacgtt
     8881 aatcaagcat acacacaacc cgagtttgta aaaacgcctt atgcaagaaa acttatgtca
     8941 aacttttttt ggcaaacaaa gcatcatttt ctaccacaca tttcctttga acttatcttt
     9001 atggaaaaac caagcaagga tacgtcacat atacccttcc caaaccgtgt acattttcta
     9061 aagaaaatct acaaaacact tgtaaatcgt gtcgagcaaa atctctctgc tccagtaaaa
     9121 gaagggaaca aaaatgatga tatctacggt gcgcagggct tgtatttacc ctaccgtttt
     9181 gatataccaa acccagtttc aaaacgagtc gatgttttac taaacaaaaa aaagggtaaa
     9241 aatgcaaaca atctcaatct tataaaatac acagtctgta ttcttgctgt ccttgattgg
     9301 tggataaata gcaaagaaag tcctgcaaac aaagaaacgg taaaaacacc ctatagactt
     9361 tcgccagaag atgggacacc tgtattttat gtaacagagc gaaaagattt agataaagtt
     9421 tttatacaaa atgtaaaagc aaagctttta cacaaagaag cacaggaaaa agaaaaagag
     9481 taaaatacct agacctgtct tcccaactgc atttttttat caaagcgctc tttttctttg
     9541 ccttcgagtt tcccgttttt attttcaagt atgagtttga gctcttcaaa ctcagctttt
     9601 gagaaattta ccccaccaag cttgtagttt tcaaaagaag cacaggcaaa gggagaaact
     9661 ttttttgcta tctcaaaaag tacctttgca taatcacgta tttctttttg tgcatgagca
     9721 tcaagacgta agcgtaaaaa atgaaaaaga ttgtgtaaat caatttgcca gtacatttcg
     9781 gtgtacaagg ataagggtaa gttaatacgt gcaatttcac gagcaagtcc tgtatcaatg
     9841 agctttgaat actcagcata ggaatttttt tgcccttcac ctaagctttc ttgtacttgc
     9901 tttgcaaaat ctgcatcgct ttcttctgaa gcacgcccct gcttattgtc tttgttttgt
     9961 agcgcgatat cttcagaggc gggaatataa aaatcatcct tcattacaga ataacgccct
    10021 gaaatttcat tcaaacgcgc ggtacgatgg cgaagccatt gacgggcaac gaaaataggc
    10081 atcttgatat gaaaggtaaa aaccacttgc tcaaaaggag aggtatggtc atttcgaagt
    10141 aaataatcaa tcaaacctgc atcttctcga acagttttag ttccctcgcc ataggaaacg
    10201 cgagctgctt gtacaattcg cgcatcccca ccgagataat caacaaggcg cacaaagccc
    10261 ttatccaaaa ccttatattc tttatccaaa atagcttctg cctcaggaac aatacaccgt
    10321 gccatacata cctcttcttt atttttttta gaccttttct aaagcctgcg taatatcagc
    10381 gataatgtca tctacatgtt ctaaaccaca agaaaaccga atcattccag catcaatacc
    10441 tgctgcagaa agctgctcgt cagtgagttg cctatgcgtc gcagaagctg gatgtaaaac
    10501 acaggtgcga atatcagcta cgtgaacaac atttgatgca agagaaagtg aatccataaa
    10561 gcgaaccgct gcttctcttc cgccttttat cgtaaaattt ataacgccac ttgatccatc
    10621 agaaagatat ttttgtgcga gcgcataata tctatcattt tttaaaccag gatagcgtac
    10681 tttttcaatt ttatctgatt ttgccaaaaa ttctgcaact tgcatagcat ttacacaata
    10741 ttctttcatg cgtaaatgca aggtttctaa gccaatgtta agcaaaaatg ccgaatgcgc
    10801 tgagggatag gagccaaagt cccgcatcaa ttgcatacgc gcttttataa tataggcaag
    10861 agaaccaaag gcttctgtgt aaactatccc gtggtagctt tcatcgggtt cggtaaattc
    10921 aggaaagttg ccgtttttcc agtcaaattt ccccgagtcg ataataacac cgccacattg
    10981 caaggcatga ccgtcgagat attttgacgt agaatgcgtt acaatatctg ctccaaagtc
    11041 tataggttta cataaaatag gcgtaggaaa ggtattgtcc acaatgagcg gaaccttatt
    11101 tttatgtgca aattttgcaa atcgttctat atccaagaca gttaaggctg gattcgtgag
    11161 tgtttcccca aaaacagcgc gggtattttc cttaaatgca gcttctagct cttcatcgct
    11221 tgcatcaggg ctcacccaaa tacactcaat accaaatttt tttagagtaa ccgcaaaaag
    11281 attgactgtt cccccataaa tccctgtcgc ggctataaaa ctgtcgccag cattacaaag
    11341 attaaggata gcaaaaagat tcgctgcctg accgctggaa gtaagcattg ctgcaagacc
    11401 accttcaagg gctgcaagtt ttttttcaac ggcatcacag gtaggatttg caaaacgaga
    11461 atacataaat tctgtcggca tatcaaaaag agatgctata tgctcggttg aatcaaagcg
    11521 gtaagttgta ctttgtacaa tgggcattac tcgaggttcg ccattttttg ggctatagcc
    11581 ctcatgaata cattgagttt caattttcat tttgcctcca aaaatatacc tagtattcta
    11641 ctagaaattc ttaatccatt caagaagttc taagaattct acgccttttt ctggataaaa
    11701 atgcttgccg tctggaagat ccatccattc tctttcaaca caagctaaga tagcatcgaa
    11761 gtcagcatca tcgagaaaaa catcaagaat agggcttttt gcatcagtct ttttgcgata
    11821 aaactgcgaa tatatatttt tttggtcttg tttgcttgta tttatcaagt ataaacgcca
    11881 caaaagccgt gcagaataac gccttgtcgc ttcatcattc gcttttattt tttcctgttc
    11941 ccgtataccg acttcagaaa ggggagaaaa caggtcgagg ttttgcaaat gcaaatacaa
    12001 atcgctcggc ttatattttt ttccaccagt aatacattca aaggcagaag aattatcgtc
    12061 aatcacttgt acaagttctt gcagagtcaa gctattcttt ttaaggtagg agtcaagatc
    12121 tttagaagtc gactctttca cttctcgcaa tgcccaagct ctgtttcgaa tatttccata
    12181 cactgattga tacatgggtt cagcaaggcg aaaggcagaa tcaaaagagc tgacagcctc
    12241 cgcatagagt gagttttgaa aaaacgaaag cgcttgctct atacgcaata cattcgaact
    12301 atctctatcg agtaattctt tagaaaagga ttctatctcg ccaagtcttt ttttgagcac
    12361 atagacttgc aaatcatagg gattctgctt ctcggcatcc tgcaaggcct tttgagcgag
    12421 ttttttttgg ctttttgcag tctgtccctg tgcatacaat agatgcaatc gcccctttaa
    12481 agcatagaga cgcgcctttg agttttgatc tggctcttgc actaaaaagg cttcgatttt
    12541 ttgcaaaagg ctcgtatagg cttcatcaga gagacggtaa cccgtatgac ctgcatgcgc
    12601 agcttcaata tccaaggcat caagactaat aagctctgat tccaagaaaa gcaattcttg
    12661 gccaagctct gtttgcggac ttgcgatata caaatctctc tgtatacttg cacaggaaaa
    12721 taaaaacaga accaaaacta gataaaaggg gcttcgtttt ttcattagtc taccatccac
    12781 gcatttattt tattatctgc cgataagaca agcatgtcat tttttttatc gccatttagg
    12841 tccacaaaaa caggaacata cataccagat agaggtgaaa aagataattc atccaagttc
    12901 tcatcgaagg caaaaagaag agaagcatct ggatttataa aaagcgcatt ttttttatct
    12961 ctgtattgat gaacggaaag atttgcattc tttgctgtaa aaccaatatc acgctgtgca
    13021 agcagccttc catccagacc aatcttatac acaatgcctg actcgtcaat tgcaaaaaag
    13081 gcatctgcaa aggcaatgag gttttctcga aacacagctt ctatttgcct cgggaagcct
    13141 tctagtaaag aaaaatcttt ccaaatgtat agatctcccg cttgcccaat aaaagcaata
    13201 taaaccgaac gcgatttttc taaaatagca ggagagccaa aaccaatact ttcaactcgt
    13261 aaaggatttt ctgcgtttaa gcagctctct tcatcaaaga caaagatatc gcctaaaaaa
    13321 ctttttgcat aaaaggcagc aagcctatct gtttttgctg gacttgaaac gaaattcccc
    13381 atagtatcaa gtgttttttc ttctagcgtt ccctcagctg acacaaagac aacagtattt
    13441 ttttctgtag gaaataaaaa cgagtccccc aaactaattc caggtgcagc tattttttct
    13501 ccagttaaaa caggaaaacc agcttctgtt tcaagctcag agtttatttt atacactgtt
    13561 ccagaagtac ttaaaaccca tagaaaagag gcttcggcat cagctgcaag aacatgaatg
    13621 ttttcagaaa actcaaaatt ctttttttgc aaggtcttta agtccaaggc aaagagagtc
    13681 cgattttttt caacccaaaa taaagttgga tttttacttt tttcaatcaa tacaaggttt
    13741 gagcttgctt taccgtccag gtcaatggga aagccatgaa tagcacgaga ttttgtcttc
    13801 gctcctgact ttgcatgcaa ggacgtaaca agccgttgat cagaaacctg cacttcacct
    13861 cgccccacag aatacagctg caaaacctgc gccaaaatag aatcaccctg cgcaaaaaaa
    13921 ggtagcgtct tttctaaatc ata
//
LOCUS       DAOSSW010000047        13574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_4623_length_13574_cov_7.322213, whole genome shotgun sequence.
ACCESSION   DAOSSW010000047 DAOSSW010000000
VERSION     DAOSSW010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 13574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13574
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4623_length_13574_cov_7.322213"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PKW26_05255"
     CDS             complement(<1..530)
                     /locus_tag="PKW26_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOQ93006.1"
                     /translation="MKKKWLLVLAFLGAFFYTYSETIHFSGDSLVAKTSENSEYTSLD
                     GNAEVKTDDLEIQADFIEISGKDFRFITASGRVSGKNLTSNFSFSCERLHYDRITKVA
                     LLESAVELHDTENDVQISAQSIEYNQKTDIATMQIEVRLTSKDSICTSALAIYNKAQQ
                     RVVLSGSPKIERNKDI"
     gene            complement(527..1153)
                     /gene="lptC"
                     /locus_tag="PKW26_05260"
     CDS             complement(527..1153)
                     /gene="lptC"
                     /locus_tag="PKW26_05260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOQ93007.1"
                     /translation="MKHRFFIRFLLSLCIFFFCSCSLIYRTELSEEEESELSLYDLVM
                     TKVNDGKKESLIKAKQMEQYRKDEHMYAKDVSFILYDKNEAIETEGSAGFMKSEKNEL
                     LVFSGGVSLTSYKEDVYIEAENLRVDTEREQLTSAEDDTILIEYGINEGKNTKLFIHA
                     QTIAASGVSNTIRISGKVSGLIIVDDENRESNQLETVDFKDANERGIE"
     gene            complement(1143..2750)
                     /locus_tag="PKW26_05265"
     CDS             complement(1143..2750)
                     /locus_tag="PKW26_05265"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOQ93008.1"
                     /translation="MSKLIIVTGGVCSSLGKGVATASLASLLEKAGLKIALMKCDPYI
                     NVDAGNMSPYQHGEIYVTDDGTETDLNLGNYSRFTNVSLSKHNSVTTGKIYEEVIKNE
                     RAGRYNGRTIQVIPHITDEIKRRILYLASSNEADIVIVEIGGTVGDIESIPFLEAARQ
                     LIREKGRGNALSIHLTLVPMVSGNELKTKPTQHSVKKMQEIGIQPDILLCRSESELPE
                     DLKHKIALFCNVEKDAVFTSIDVEQSVYELPVYFHKQGLMQKALQKLNINDTVSDISS
                     WNLFLRRFNNPKQSLKVGMLGQKNYLDDCYKSVRESLLHAAVTGNNASLEIIKIDAET
                     IESCTNPKDYFKDIDAIIIPGSYGQRGFLGLLATVQYAREQKIPFLGIDLGMQLMAIE
                     AARNILGWQEADSTEFIQNTEYPIISLPEEQAGLTAAGVMRLGAMEVRLLKGSLIESV
                     YKSTTIMERHRSKYAFDKKYTEDLEKTGLSITGFSMKDNSVEVFEWKDHPWGIGVQFH
                     PEFTSQPSKPHPLFTAFIKSALKPHET"
     gene            2839..3654
                     /locus_tag="PKW26_05270"
     CDS             2839..3654
                     /locus_tag="PKW26_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOQ93009.1"
                     /translation="MADLLTDSDFDLFRKVIYDESGITFSSSNRSILDSRLKERLRET
                     QLPHVQDYYKLIMSDKEEMKTMLDAVTTNLTRFFRNQPHFDAFINYVVPHIIQYKQGK
                     PDTKVRVWSAGCSTGEEPYTIAMILKDILKPPYSFEIVASDLSLKSLMVGQQGFYSDA
                     RIVGIPDAYLARFFTKVEGGYQVKDEIKNTIRFDYHNLKHDAGLRNLDVIFCRNVLIY
                     FDEAAQKAVVNKFYDSLAQKSFLYIGHSESLFGMNTKFEFLKTDWATLYQKNE"
     gene            3665..>4151
                     /locus_tag="PKW26_05275"
     CDS             3665..>4151
                     /locus_tag="PKW26_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ93010.1"
                     /translation="MDTISVLVVDDSALMRNLIGRIVEQTPGLSLAGKAMNGQFALDK
                     IPMLKPDVIVLDIEMPTMNGVEFLKERKKRGIQIPVIILSSIAKEGAAVTLECLSLGA
                     SDFITKPSGSDSADISKVAQKLAELLLHFGAEYARKYAKKQLDFSAKQKTDVEKTEGA
                     KD"
     assembly_gap    4152..4251
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4252..4620
                     /locus_tag="PKW26_05280"
     CDS             <4252..4620
                     /locus_tag="PKW26_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HOQ93011.1"
                     /translation="ILIAPGNKHMTVEQRSLASIVHINEHDQVNGHRPSVDVLFESIA
                     QQYKNKALGVVMTGMGKDGAAQLAEMRKQGAYTLAQDEESSVVYGMPKVAYEYGGVQK
                     QIPLKDMAKEISKLAKEKAV"
     gene            complement(4651..5076)
                     /locus_tag="PKW26_05285"
     CDS             complement(4651..5076)
                     /locus_tag="PKW26_05285"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOQ93012.1"
                     /translation="MRDRASVIAIDGKKITVVPIITDTCIGCEKSSCGERGEPFFVTN
                     PQNLSLSIGSSVKIHARAKDQMLQALFAIFLPVLLAILSFVLIGALAHSLGRQVNTES
                     IERLKTLGAIGILFISSLLIMLKNRFFPPRGQSEIIAVL"
     gene            complement(5069..5605)
                     /locus_tag="PKW26_05290"
     CDS             complement(5069..5605)
                     /locus_tag="PKW26_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ93013.1"
                     /translation="MTENPEHLNIQAIELAAQGDFPEALACLRRAIALEKENYLLWFN
                     MGITYRDSGDLESAKRAFKQANTINPEDEEVLETLALTYFSLGAIDKAFAICEDCLEL
                     NPYNAQIWNTIGVMNFTHENYEEAAASFEKAVSIDPHYFDALYNLRDTYEELGNEEAA
                     KECDDKLKMIKMPGYFYA"
     gene            complement(5680..6765)
                     /locus_tag="PKW26_05295"
     CDS             complement(5680..6765)
                     /locus_tag="PKW26_05295"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOQ93014.1"
                     /translation="MNQYTAKRKKIEDAIETFFQTIDTSAYAKLPRTIQEKHLLELKN
                     PCMSLVSQGGKRWRPLLLVLCAELFLNKEQEKNTDALTKISAEERLEKAYAISPLVEL
                     IHTASLIHDDIEDASDTRRGLAACHISYGLDTALNAGSWLYFAALNAIETSLSTKAED
                     AALAYRLLNYSLTDIRRLHLGQAMDIAWHKESALIPSSLEYEAMIALKTGSLARLSAI
                     IGASIGGASEKEALSLGELAMQIGIGFQIIDDYTNLTIGNPGKKRGDDIVEGKKSYPI
                     LLHLEKKPEDLAKIAEYFQNARVEGIDSPSVEKCIAIINTSDALKEAKEKGEALILDS
                     CNKIVYLYDNNEAAKEIQQLFFEMLLS"
     gene            6818..7417
                     /locus_tag="PKW26_05300"
     CDS             6818..7417
                     /locus_tag="PKW26_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HOQ93015.1"
                     /translation="MAGNSYEKPDFWSKKAFSEGYPARSVYKLQEIDEKFHILKKNAR
                     ILDLGAAPGSWTVYVLRYLGASGQVTSIDLNPLAKEVKGDNLTFIQGDLYDERVRNHA
                     AALGPYDALICDAAPPTTGNKLVDTARSADLVELALFYAQTMVKKGGNCAVKIFQGGD
                     QSILLRQMREIFQSAKGFKPKACRSESFETYLIGLDKKN"
     gene            7427..8404
                     /locus_tag="PKW26_05305"
     CDS             7427..8404
                     /locus_tag="PKW26_05305"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ93016.1"
                     /translation="MNNTTMKSIASHLISFGFIVLFLFSLAACKKQGLGQGGFESPGR
                     EVSRQDFLEKGTALTKTFSGPSLSTGALPLETVRDETALEYSVYRVKSGDMIGVLADE
                     FGLTQDTLISVNAIRQTRLLQVGQYLKIPSMSGIIYTVRTDGETIEAIAKKYEVDEKK
                     CAKTNNLPVSQSLNAGTALFIPDAELDWVTRQEINGDLFIRPLRSSYYFSSYFGWRNS
                     PFTGQRTFHNGIDMAAPRGTYIYPALAGTVTTVSYDNVYGNYVIVTHHSGYKTLYGHM
                     SETLVRKGQYLTTESRLGRVGSTGMSTGDHLHFTVFKNGVAVNPQALWR"
     gene            complement(8449..8919)
                     /locus_tag="PKW26_05310"
     CDS             complement(8449..8919)
                     /locus_tag="PKW26_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93017.1"
                     /translation="MQKKEVCEKIIEDYLLLDRGEAVPLKLWTHLLFCKDCRTEVRFL
                     NLADTACTKPLTIERKKEESLAKTIISRIQNDPECKEIFVPKELTLKNWIISGLIMLF
                     AMLFFTINIYSQNSQIALPVYLVFAVFISGYCALFVGSNMDFFIKKLHKLEIQF"
     gene            complement(8938..9552)
                     /locus_tag="PKW26_05315"
     CDS             complement(8938..9552)
                     /locus_tag="PKW26_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOQ93018.1"
                     /translation="MKKTKSKPIKIQTQSLERTIIEKRDKALIRSACKGDHLAFEKLL
                     RLYENRVRILGLSFFKNEIDTDDFIQEVFIKVYKNLSSFKGESLFSTWLMRIAYNTAI
                     NAINRRKEYLSLSEDFELVDLDFGPEEQELRKHTKAAIQAAMGELPDRYKACLDLYFF
                     YDMSHKEISSVLDLPINTIKSHIFRAKKILRKNLEGFVENTNQR"
     gene            complement(9571..9897)
                     /locus_tag="PKW26_05320"
     CDS             complement(9571..9897)
                     /locus_tag="PKW26_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93019.1"
                     /translation="MVPSAQIIVSIIPIIGILMGGAILFFYLLWRHKQVSLLIKTNSY
                     KPARVDLRIFSLLTGLLLSGTGFILSLLFFLLEGLSYSLLGGLIPCVLGLALIAFYFL
                     YPKDIK"
     assembly_gap    9901..10000
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10160..10633)
                     /locus_tag="PKW26_05325"
     CDS             complement(10160..10633)
                     /locus_tag="PKW26_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93020.1"
                     /translation="MTKFQKILMFIAAILFFSLALLSLVRFVFFPEQVGTAFRKKEAT
                     IAQAEAKEEKSERGVFSSIGQLRLSTEDADDFIIILRPYFFYPKSDADFFEELVTKNQ
                     KMRFLITEYFSKKTKDYIISTKEANIKSDLLSILNEELVLGYITELYFDEYIFLD"
     gene            complement(10675..11988)
                     /gene="hflX"
                     /locus_tag="PKW26_05330"
     CDS             complement(10675..11988)
                     /gene="hflX"
                     /locus_tag="PKW26_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOQ93021.1"
                     /translation="MASTYNTQEEPIRAFLVGLDDVEHNIGELQGLVHTLSFEIAESY
                     ILPPFKPHSQFLLGSGKAKEIHARVQSLDADCIIFDFEISPTQQRNWERLTHIPVFDR
                     NEVIIRIFGERAQTKEAKLQVELARLQYSLPRLAHSYGDMARQRGGSYGSKGTGETQL
                     ELDRRGIQKKIAKIKLELQKVIKERDTMRKKRENIPLPNAALVGYTNAGKSSLLNVLT
                     GSDVFVEDSLFATLDPTTRRLNLSGKTGLLLTDTVGFIKNLPHSLIDSFKATLEEAVR
                     ASLLIIVLDSSDPDIENQYETVCSVLEEIGAFQKERIIALNKIDLLDEKAKSEDSVFF
                     DQNDKKLFFSYLEKTEKHLHFISSHTKEGLKELTETIFDLTYGLEKTFHLSLHDTALI
                     DEIRNTGVLVSSEWTDTAIIVKARVKPNLEAKILGETYQEDKGFF"
     gene            12104..>13574
                     /locus_tag="PKW26_05335"
     CDS             12104..>13574
                     /locus_tag="PKW26_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93022.1"
                     /translation="MSSGKKVALSLFISIIAFSAFIFFAYTDFFQAIEVHFYQPMVLD
                     SYEKKLDQLSEAFNSFLDLKKRSVLAFASETAVRNTASPTQTKEDIDTIENITGSLLT
                     EGSGLLGIRLIGHDGRKIHYSSFSQDLLRKEENRLQYESYIKLDELDYEEIACAKESD
                     FELTFDSERDRFLISLPFFDTFNIYQGSIVFYYSALSVQQLFISENLISLSETITFSK
                     KGIVLGLAEKGQKEIKDYLESIFAEPYQRLYPQFAINEENFLFLSHETQFNNQKIIFS
                     QLIREDFLVFNDTIKIVLLVCFFISFFLVILFLLNLKSDASLVIRQRIKKFQFALLNE
                     YLETKEKINWNDVIASIESRKVEVALSLTKSLGRKAKKHQDLLDALLEKSWDEIMASL
                     TRSKNEFVQNVHIDTDEIRAMLEEILLSTRQGGEKLARLPQEKSKPAVISSEVEELDE
                     IAELDEVEELDEIAELDEVEELDEIAELDEVEELDEIAEL"
BASE COUNT         3784 a         2545 c         2749 g         4296 t
ORIGIN      
        1 aaaatatcct tgttccgctc tatttttgga ctcccgctca agacgaccct ttgttgcgcc
       61 ttgttataaa ttgcaagggc actcgtgcaa attgagtcct tgcttgtaag acgtacctct
      121 atttgcattg tggcaatatc tgtcttttgg ttgtattcga tactttgtgc acttatctgc
      181 acatcatttt ctgtatcgtg aagttcaaca gccgattcta agagggctac ttttgttatg
      241 cggtcataat gtagtctttc gcaagaaaag ctaaagttcg atgtgaggtt ttttccagaa
      301 actcgcccac ttgcggtgat aaaacgaaaa tctttcccag aaatttcgat aaagtctgcc
      361 tgtatttcaa ggtcatcggt tttgacttcc gcgtttccat ctaagcttgt gtattcgcta
      421 ttttcacttg tttttgcgac aagggaatcg ccgctaaaat gtatggtttc tgagtatgta
      481 taaaaaaatg ctcccaaaaa ggctaggacc aaaagccatt tttttttcat tctattcctc
      541 tttcatttgc atctttaaaa tctactgttt ctagttgatt gctttctctg ttttcatcgt
      601 caactatgat taaaccgctt acttttccgc ttatacgaat cgtattgctt acgccactcg
      661 ccgctattgt ttgggcatgt ataaaaagct ttgtgttttt tccctcattt ataccgtatt
      721 cgattaaaat agtatcatct tctgcacttg ttagctgctc acgttctgta tcgactctca
      781 gattttctgc ctcaatatag acatcttcct tgtatgaagt gaggctcaca ccaccagaaa
      841 aaactaaaag ctcatttttt tcggatttca taaagccggc gcttccctct gtttctatcg
      901 cttcgttttt atcatacaag ataaacgaca cgtcttttgc atacatatgt tcatcttttc
      961 gatactgttc catttgcttt gctttaatga gactttcttt tttcccatcg tttacctttg
     1021 tcatcaccaa atcatagagg ctcagctcgc tttcttcttc ttcactgagt tctgttcggt
     1081 atatgagaga gcaggaacag aaaaaaaaga tacagaggct cagtaaaaat cgaataaaaa
     1141 atctatgttt catgcggttt taaagcactt tttataaatg cggtaaatag tgggtgcggt
     1201 tttgatggct gggatgtaaa ttctgggtga aattgcactc caatgcccca gggatggtct
     1261 ttccactcaa agacttccac ggaattgtct ttcatagaaa accctgtgat actgagacct
     1321 gttttttcca aatcttcggt atattttttg tcaaaggcat attttgatcg gtgtctttcc
     1381 attatggtag tcgatttata cacagattct atgagactgc cctttagcaa acgaacttcc
     1441 attgctccaa ggcgcataac tcctgccgct gtcagccctg cttgttcttc ggggagggaa
     1501 ataatgggat attccgtgtt ttgaataaat tccgtcgagt cagcttcctg ccatccgagg
     1561 atatttcttg ctgcctcgat tgccatcaat tgcatgccta agtctatacc taagaagggg
     1621 attttttgct cgcgtgcata ttgtactgtg gcaagtaagc ccaaaaagcc acgctgacca
     1681 tagcttcctg gaataatgat ggcatcgata tctttgaaat aatctttggg gtttgtacag
     1741 gattcaattg tttctgcatc tatttttatg atttcgagcg aggcattgtt tcccgtaact
     1801 gctgcatgga gaagagactc gcgaacagat ttataacagt cgtcgaggta gtttttttgt
     1861 ccgagcatac cgacttttaa gctttgtttt ggattattga atcggcgtaa aaagaggttc
     1921 caacttgaaa tatcagagac agtgtcgttt atattgagtt tttgtagggc tttttgcatt
     1981 aaaccttgtt tatgaaagta aacgggaagt tcatagacgc tctgttcaac atcgatagaa
     2041 gtaaagactg catctttttc aacattgcaa aaaagggcaa ttttatgttt taagtcctcg
     2101 gggagttcag attcgcttcg gcaaagaaga atatcgggct gtataccaat ttcttgcatt
     2161 ttttttaccg aatgttgggt aggttttgtt ttaagttcat tgccactcac catggggacg
     2221 agggtgagat ggatagaaag tgcatttcct cttccttttt ctcgaatgag ctgacgggct
     2281 gcttctaaaa aggggatgga ttcaatatcg ccgacagtgc cgcctatttc aacaataaca
     2341 atatctgctt cattgctcga agcaagataa aggatgcggc gttttatttc atcggttata
     2401 tgcggtataa cttggatggt tctgccattg tagcgcccag cacgctcgtt ttttataact
     2461 tcttcgtaaa ttttccctgt tgtaacggaa ttgtgctttg aaagactcac atttgtaaat
     2521 cgtgaataat tgcctaaatt aaggtctgtt tctgtaccgt catcggtaac gtagatttca
     2581 ccatgttgat aggggctcat attacctgca tcaacgttta tataggggtc acatttcatt
     2641 aaggcaattt ttaaaccagc tttttccaag aggcttgcca gagaagctgt ggcgacgcct
     2701 ttacccaaac ttgaacagac accaccggtt acaataatca atttactcat ttttcaattt
     2761 tcccatctca ggggtttttt gtcaacatga agcgataaaa tcatttactt tttattgaag
     2821 ctactgtaca atctctgtat ggcagattta cttaccgatt ccgattttga tttatttaga
     2881 aaggttattt atgatgaaag cggtattacc ttttcctctt caaaccggtc tatcttagat
     2941 agcaggctaa aagagcgctt gcgtgaaaca caattacctc atgtgcaaga ttattacaag
     3001 cttattatgt ctgacaaaga agaaatgaag acaatgctcg atgctgttac aacgaatctt
     3061 acacggtttt ttagaaatca accgcatttt gatgcattta tcaattatgt agttccgcac
     3121 attatacaat acaagcaagg aaaacccgat accaaggttc gtgtttggag cgctggttgt
     3181 tcgacaggag aagaacccta tacgatagct atgattctca aagatatttt aaaaccgcca
     3241 tatagttttg aaattgtagc ttcggacctt tctttgaaat cgcttatggt aggacaacaa
     3301 ggtttttatt ctgacgcccg tattgtaggt atccccgatg cttatttggc acgatttttt
     3361 actaaagtcg aaggtggcta tcaagttaaa gatgaaataa aaaacactat tcgatttgac
     3421 tatcataact taaaacacga tgcaggattg cgaaacctcg atgttatttt ttgtaggaat
     3481 gtattgattt attttgacga ggctgcacaa aaagctgtgg taaataaatt ttatgactcc
     3541 cttgcgcaaa aatcgttttt gtatattggg cattcagaat ccttgtttgg aatgaataca
     3601 aagtttgagt ttctaaaaac agattgggca actttgtatc aaaagaatga ataggacgta
     3661 aacgatggat acaatatcag ttttagtagt cgatgattct gccctcatgc gcaatctcat
     3721 tgggcgtatt gtagagcaaa caccagggct ttctcttgca ggaaaggcaa tgaatggaca
     3781 gtttgccctc gataaaattc ctatgctcaa acccgatgtt attgtactcg acatagaaat
     3841 gccgactatg aatggtgtgg aatttctaaa agagagaaaa aaacgtggca tacaaattcc
     3901 cgttattatt ttatcgagta ttgcaaaaga aggcgcagca gttacgctag aatgcctttc
     3961 tttaggagcg agtgatttta taacgaagcc ctctggctcc gactctgcag atatatctaa
     4021 ggttgcgcaa aaacttgcgg aactgctttt acactttggg gcagaatatg ccagaaaata
     4081 cgcaaaaaaa cagcttgatt tttctgcaaa acaaaaaact gatgtagaaa aaacggaagg
     4141 cgcaaaagac annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natcctcatc
     4261 gccccaggaa ataagcatat gacggtcgag caacgttctc ttgcatcgat tgtacatata
     4321 aatgagcatg atcaagtaaa tgggcatcgt ccttctgttg atgttttatt tgaatctatt
     4381 gcacagcagt ataaaaacaa ggctctcggt gtcgttatga ctggaatggg aaaagatggg
     4441 gcggcacaac tcgccgaaat gcgaaagcag ggcgcctata cactcgcaca agatgaggaa
     4501 agctcagttg tctacggtat gccaaaggta gcctacgaat acggtggtgt tcaaaaacaa
     4561 attcctctca aagatatggc caaggaaata agcaaactcg caaaagaaaa ggctgtctga
     4621 gttttagtat ttcattagtg taaaactagt ttacaaaaca gcgataatct cactttgtcc
     4681 ccttggtggg aaaaaacggt ttttgagcat aataagaagg cttgaaataa ataaaatgcc
     4741 aattgcccct aaggttttaa gtcgctcgat agactctgta tttacttgtc tgccaaggga
     4801 gtgagcaaga gccccaataa gtacaaaact gagtatagcg agaagaacag gtaagaaaat
     4861 agcaaaaagt gcttgaagca tctggtcctt tgctcttgcg tggattttta cactggagcc
     4921 aattgaaagg gagaggtttt gagggtttgt tacaaaaaaa ggttcccctc tttccccaca
     4981 actgcttttt tcacaaccga tacaggtatc agttataatg ggtacaacag taattttttt
     5041 tccgtcaatg gctataacgc ttgctctatc acgcataaaa atatcctggc atctttatca
     5101 ttttcagttt atcgtcgcat tcttttgcag cttcttcatt tcccagctct tcgtaggtat
     5161 cgcgtaggtt atagagtgcg tcaaagtagt gcgggtcaat acttaccgct ttttcaaaac
     5221 ttgctgcagc ttcttcataa ttttcatggg taaaattcat cacgccgata gtattccaaa
     5281 tctgcgcatt gtagggattg agttcgaggc agtcttcgca aatggcaaag gccttatcga
     5341 ttgctccaag agaaaaataa gtgagcgcaa gtgtttcgag gacttcttca tcttctggat
     5401 tgatagtatt tgcttgtttg aaagctcgtt ttgcactctc taaatctcca gaatcgcggt
     5461 aggttatacc catattgaac cataaaagat agttttcttt ttctagggcg atagcccgtc
     5521 gtaggcaggc taaggcttcg ggaaaatcac cttgagcggc aagttctatc gcttgaatat
     5581 ttaaatgttc agggttttct gtcatagcta ctcctcaatc taataatagt ccattttccc
     5641 caaaaactca ataaagtctt tgccgtcatt gcttaaagct taagaaagga gcatttcaaa
     5701 aaataattgt tgtatttctt ttgctgcctc attgttatca taaagatata ctattttgtt
     5761 acaagaatca agaataagtg cttcgccttt ttcctttgct tccttgagcg catctgaagt
     5821 gttgataatt gcaatacatt tttcaacact gggcgagtca atcccttcaa ctcttgcgtt
     5881 ttgaaaatac tctgctattt ttgcaaggtc ttcgggtttt ttctctaggt ggaggagtat
     5941 gggatagctt tttttccctt cgacaatgtc atcgccgcgc tttttccccg ggtttccgat
     6001 cgtgagattg gtataatcgt cgatgatttg aaagccgata cctatttgca ttgcaagttc
     6061 tcccaagctc agcgcttctt tttcgcttgc tcctccgatg cttgcgccga ttatggcaga
     6121 aagtcgcgcg agacttcctg ttttaagggc aatcattgcc tcgtattcta ggctacttgg
     6181 tatgagggcg ctttctttat gccaagctat gtccatcgct tgcccgagat ggagtcgacg
     6241 gatatctgtg aggctgtagt ttaaaaggcg ataggcgagg gcggcatcct ctgcctttgt
     6301 cgaaaggctt gtttcaatgg cgtttagtgc cgcaaagtaa agccaggagc cagcattgag
     6361 ggcggtatct aagccgtagc ttatgtggca ggcggctaat ccgcggcgtg tatccgaggc
     6421 atcttctata tcgtcgtgga tgaggctggc ggtatgaatg agctcgacta gggggcttat
     6481 cgcataggct ttttccagcc tttcttcagc gcttattttt gttagggcat ctgtattttt
     6541 ttcttgttct ttgttgagaa agagttcggc gcagagtacc aaaagtaagg ggcgccatcg
     6601 ctttcctcct tgggagacaa ggctcataca gggatttttt aattcaagaa ggtgtttttc
     6661 ttgtattgtc ctaggtagtt ttgcataggc gcttgtatcg atggtttgaa agaaagtttc
     6721 tattgcatct tctatttttt tacgttttgc agtatactgg ttcatggtac agagtatatg
     6781 atatataaat ataaattgaa ataggggaag gattttcatg gcaggaaaca gctacgagaa
     6841 gccagatttt tggtcaaaaa aagctttttc agaaggctat ccggctcgct ctgtatataa
     6901 attgcaagaa atagatgaaa aatttcacat attgaaaaaa aatgcgcgta tactagacct
     6961 tggtgctgct cccgggagtt ggacggtcta tgttttgcgt tatctcggtg cttcggggca
     7021 ggtgacttct attgacttaa acccgcttgc aaaagaggtc aaaggagata acttaacctt
     7081 tattcagggc gatttatatg atgagcgagt gagaaatcat gcggcggctt tgggacccta
     7141 tgatgcactt atttgtgatg cggctccccc cacgaccgga aataagcttg tcgatactgc
     7201 tcgttctgcc gacttggttg aacttgcgct tttttatgca caaacaatgg taaagaaagg
     7261 cggaaattgt gccgttaaaa tatttcaggg gggagatcaa agtattttgc taaggcagat
     7321 gcgtgaaatc tttcaatccg caaagggttt taaaccaaaa gcttgccgaa gtgaatcctt
     7381 tgaaacctat cttattggct tagataaaaa aaattaaggg aaaaaaatga ataatacgac
     7441 tatgaaatcc attgcctcac atcttatctc ttttggtttt attgttttat ttcttttttc
     7501 tctcgctgcc tgtaaaaaac aaggccttgg tcagggtgga tttgaaagcc ctggtcgaga
     7561 agtttctaga caagactttc tcgaaaaggg tactgctctt acaaagactt tttctggtcc
     7621 ttccttatcg acaggggcgc tccctcttga gactgtccgt gatgaaactg ccttggagta
     7681 ttcggtttat cgtgtaaaaa gcggcgatat gattggcgtt cttgccgatg aatttggctt
     7741 aacgcaggat acgcttatct cggtaaatgc gattcggcaa acacggcttt tacaagtcgg
     7801 tcaatatcta aaaattcctt cgatgtctgg tattatctac acggtgcgaa ctgacggaga
     7861 aacaatcgaa gctatagcaa aaaaatatga ggtcgacgag aaaaaatgtg cgaagacaaa
     7921 taatctgcct gtttctcaaa gtctcaatgc cggaaccgct ctttttatcc cggatgcaga
     7981 gctcgattgg gttactcgcc aggaaatcaa tggggactta tttatcagac ctttgcgcag
     8041 ttcctattat ttttcttcgt attttggttg gagaaatagt ccgtttacgg ggcaaagaac
     8101 ctttcacaac ggcattgata tggcagctcc tcgcggaacc tatatttatc cagctttggc
     8161 aggtactgtt acaacagtct cctacgataa tgtatacgga aattatgtta ttgtgacaca
     8221 tcactcaggc tataaaacct tgtacgggca tatgagcgaa acgcttgttc gaaaagggca
     8281 gtatcttacc actgaatcga gacttggtcg ggtcggtagc acgggcatga gtacgggtga
     8341 tcacttgcat tttaccgtct tcaaaaatgg tgtggcagta aatcctcaag ctttgtggcg
     8401 ctaagcgcag tcttgtttta tctttcgtct aatgtctgaa gtaaaaactc aaaactgaat
     8461 ttcaagctta tggagctttt ttataaaaaa atccatattg cttccgacaa aaagtgcaca
     8521 ataaccagat ataaataccg caaaaacaag atagacggga agggcgattt gtgagttttg
     8581 cgaataaata ttgattgtaa aaaaaagcat ggcaaaaagc ataataagac ctgaaataat
     8641 ccagtttttt aatgtcagtt cttttgggac aaagatttct ttgcattctg ggtcattttg
     8701 aatgcggctt ataattgttt ttgcaagact ttcttctttt tttcgctcaa tcgtcagcgg
     8761 ctttgtgcag gcggtatcgg caaggttgag aaatcgcacc tccgttcgac agtccttgca
     8821 gaacagtaaa tgcgtccaga gttttaaggg aaccgcttcc cccctatcca aaaggagata
     8881 gtcctctatt attttttcac agacttcttt tttttgcaaa gctatacctc cttattttta
     8941 cctttgattt gtgttttcta cgaaaccttc taggtttttt cgcagtattt tttttgctct
     9001 aaaaatatga gatttaattg tatttatggg aaggtccaaa acagagctaa tttctttatg
     9061 gctcatatca taaaaaaaat ataaatctaa acaggcttta tagcgatcag gaagttcgcc
     9121 catagcggct tgaatcgcag cctttgtgtg ttttcgaagc tcctgttctt ctggcccaaa
     9181 atccaagtct acaagctcaa aatcttcact caagctcaag tattcttttc gccgattgat
     9241 tgcatttata gcggtattgt aggcaattct cataagccaa gttgaaaaaa gcgattcccc
     9301 tttaaagctt gaaaggtttt tgtacacttt tataaaaacc tcttgtataa aatcatccgt
     9361 atctatttcg tttttgaaaa aactaagacc gagtatgcgc actctgtttt catataaacg
     9421 gagtagtttt tcgaaagcga gatggtcacc cttgcaagca gaacggataa gcgccttatc
     9481 tcttttttcg attatcgttc gctcaaggct ttgagtttgt atttttatcg gcttagattt
     9541 tgtttttttc aaagagttaa aaggctttgt ttattttata tctttgggat agagaaaata
     9601 aaacgcgata agagcgagac cgaggacaca gggaatgagt ccaccgagga gagaatagga
     9661 aagcccttct agtaaaaaaa acaagagact caaaataaag cctgttccag aaagcaataa
     9721 gccagtgagt agtgaaaaaa tgcgtaaatc aacacgagct ggtttatagc tatttgtttt
     9781 tatgagcaaa ctcacctgct tatgtcgcca tagcaggtaa aaaaataaga tagcaccacc
     9841 cattagaata ccgattatgg gtataatcga aacaataatt tgtgcactgg gaaccatctg
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaataccgat tatgggtata
    10021 atcgaaacaa taatttgtgc actgggaacc atctgtgcag tgttcataca tcctcccaaa
    10081 gtattttttt tcgagccttc gcagccatgc gagaaggctc cgttatattt agacacaggc
    10141 atggctgaaa agttgcgtct tagtctaaaa atatatactc atcaaaatag agctccgtta
    10201 tatatcctaa aacaagttct tcattgagta tgctcagtaa gtcgcttttt atattcgcct
    10261 cttttgtgct tattatatag tcctttgttt ttttcgaaaa atactcggtt atgagaaaac
    10321 gcattttttg attttttgta acaagctctt caaaaaaatc tgcatcactc tttggataaa
    10381 aaaagtaggg gcgaaggatt ataataaaat catctgcatc ttctgttgaa aggcgcaact
    10441 gtccaatcga ggaaaaaacg ccacgttcgc ttttttcttc ttttgcctct gcctgagcta
    10501 ttgttgcttc tttttttcta aatgcagtcc caacctgttc tggaaaaaaa acaaatcgaa
    10561 ccaaagatag aagggcaaga gagaaaaaaa ggatggcggc aatgaacata agtatttttt
    10621 gaaactttgt catagtatag agtatagcaa acttattcaa aaaagataag aagattaaaa
    10681 aaagcccttg tcttcttgat atgtttctcc gagaattttt gcttcgagat ttggtttgac
    10741 gcgtgctttt acaataatcg cagtatctgt ccattcgctc gagacaagta ctcctgtgtt
    10801 tcgtatttca tcgatgaggg cggtatcatg tagtgaaaga tgaaatgttt tttccaagcc
    10861 gtatgtgagg tcaaagatag tttctgtgag ctcctttaaa ccttcttttg tatgactgct
    10921 tataaaatgt agatgttttt ctgttttttc taaataacta aaaaaaagtt ttttatcgtt
    10981 ctggtcaaaa aacaccgagt cttcagactt ggccttttcg tcgagtaaat ctattttatt
    11041 taatgcaata atgcgttctt tttggaaggc tccgatttct tccaagacag agcaaactgt
    11101 ttcgtattga ttttcaatat cgggatcgga gctgtctagg acgattatga gtaaagatgc
    11161 gcgaacagct tcttctaggg ttgccttaaa tgaatcgatg agactgtggg ggaggttttt
    11221 tataaagcca actgtatcgg ttaggagaag gcctgttttc ccagaaagat ttaaacgtcg
    11281 agttgtgggg tcgagtgttg cgaaaagaga atcttccaca aaaacatcgg agccagtgag
    11341 gacgttgagc aagctcgatt ttcctgcatt tgtatagcca acaagcgcag cgtttggaag
    11401 ggggatattt tctctttttt tccgcattgt atcgcgctct tttataacct tttgtaattc
    11461 aagttttatt tttgcaattt tcttttgaat acctcgtcta tcaagctcga gttgcgtttc
    11521 tcctgttccc tttgagccat aagagccgcc tcgttgacgt gccatatcgc cataactatg
    11581 ggcaagtcgt ggaagagaat actgtagcct ggcaagttct acctgcaact ttgcttcctt
    11641 tgtttgagct ctttctccaa aaatgcggat aataacttcg tttctatcga atacaggaat
    11701 atgtgtaagg cgttcccagt ttcgttgttg cgttggtgag atttcaaagt caaagataat
    11761 gcaatccgca tcgagggatt gcacacgagc atgaatttct tttgcctttc cggagccgag
    11821 aaggaattgg gaatgcggtt tgaagggagg caggatatag ctttcagcta tttcaaaaga
    11881 aagcgtatgt acaagccctt gcagctcccc tatattatgc tctacatcat caagacctac
    11941 gagaaaagct ctaattggtt cttcttgagt attatacgta ctagccataa gcatatagta
    12001 tactctttta gatttttttt atagtataaa agtgcttaat atatcgtcta aaaagccgat
    12061 accttgtata tctagcttta tacgctacaa ttttggagga cctgtgtcgt cagggaaaaa
    12121 agttgcctta agtcttttta tatctatcat agctttctct gcctttattt tttttgcgta
    12181 cacagatttt ttccaagcaa tcgaggtgca tttttatcaa cctatggtgc tcgattccta
    12241 tgaaaaaaaa ctggaccagc tttctgaagc atttaatagt tttttagatc ttaaaaagcg
    12301 cagtgtcctt gcctttgcct ccgaaacagc ggtgcgcaat accgcatctc ctactcaaac
    12361 aaaagaagat atagatacga tcgaaaatat aacaggttct ctcttgaccg agggtagtgg
    12421 gctcttggga attcgtctta ttggtcacga cggtaggaaa atccattata gtagtttttc
    12481 tcaagattta ttgcgaaaag aagaaaaccg cttacaatac gaaagttata ttaaactcga
    12541 tgagctcgat tatgaagaga tcgcctgtgc aaaggaaagc gactttgagc tcacctttga
    12601 ttcggagcgc gatagatttt tgatttcttt gccttttttt gacacattca atatatatca
    12661 gggttccatt gttttttatt actcagcgct ttctgtgcag cagcttttta tttctgaaaa
    12721 ccttataagc ctatctgaaa cgattacttt ttcaaaaaag ggcattgtcc ttgggcttgc
    12781 tgaaaaaggg caaaaagaaa ttaaagacta tcttgaaagt atttttgcag agccctacca
    12841 aagactgtat ccacaatttg ccataaacga agaaaatttt ttatttttat cgcatgaaac
    12901 tcagttcaat aatcaaaaga ttattttttc acagcttatt cgagaagatt ttcttgtttt
    12961 taacgatacc ataaaaatcg ttttactcgt ttgttttttt ataagcttct ttttagtgat
    13021 tttattttta ctcaacttaa aatccgatgc aagtctagtc attcgtcagc gaataaaaaa
    13081 attccaattt gctcttttaa atgaatactt ggagacaaag gaaaaaataa attggaatga
    13141 tgttattgcg tccattgaaa gtcgaaaagt cgaagttgcc ctgtctctta caaaaagctt
    13201 aggacgaaaa gcgaaaaaac accaagattt gctcgatgcg cttttggaaa aatcctggga
    13261 cgaaattatg gcttcgctta cacgatccaa aaatgaattt gtccaaaatg ttcatattga
    13321 taccgatgag attcgggcga tgttggaaga gattttgctt tcgacacggc aaggtggcga
    13381 aaaattggct cgattgcctc aggaaaaatc taagccagct gtaattagtt ctgaagttga
    13441 agagctcgac gaaattgcag aactcgatga ggtcgaagag cttgacgaaa ttgcagaact
    13501 cgatgaagtc gaagaacttg acgaaattgc agaactcgat gaggtcgaag agcttgacga
    13561 aattgcagaa ctcg
//
LOCUS       DAOSSW010000048        12937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_4846_length_12937_cov_7.995265, whole genome shotgun sequence.
ACCESSION   DAOSSW010000048 DAOSSW010000000
VERSION     DAOSSW010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 12937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12937
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4846_length_12937_cov_7.995265"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2477)
                     /locus_tag="PKW26_05340"
     CDS             complement(<1..2477)
                     /locus_tag="PKW26_05340"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOQ93023.1"
                     /translation="MKGIELEKAYNPKDFEDRIYADWEKKGYFKPKTDTDSALYSANK
                     ASESLSAPANAESFTVVIPPPNVTGVLHMGHGLNNALQDIIVRYHRMTGKNTLWLPGT
                     DHAGIATQNVVERRLKKDGLSRHDLGREAFVKKTWEVKEEHHEIITKQLKKIGASVDW
                     SRERFTMDEGLSRAVREVFVSLFERGLIYKGNYLVNWCTSCGTALADDEVEHTDKQGM
                     MYHLYYEIEGASLPAVKLEDGTEFPGGNKIEIATTRPETLLGDTAVAVHPDDPRYTAL
                     IGKKVLLPLTGRSIPVVADSYVDPSFGTGVVKITPAHDPNDWELGQRHNLEIINILNP
                     DGSLNDAVPKIYQGLSSKEARKKVLEDLVELGLYKEEKPISHAVGHCYRCDTVVEPYL
                     SDQWFVKMKPLADKALKAWKDGDIVFYPKKWENTYSHWMENIRDWCISRQLWWGHRIP
                     VWYCDNCGEMMVSRVDLSECTKCKSKEIHQDADVLDTWFSSWLWPFSTLGWPENGKNL
                     EDLASDFKTFYPTTALVTAYDIIFFWVSRMIMAGLEFTGKVPFKDIYIHGLVRDKQGR
                     KMSKSLGNGLDPLEITEEFGSDAMKFTLGFMCAQGQDILIDKESFKLGSRFANKIWNA
                     SRYLLGNLEERNLVPIQDSDLTDLDKWIYARLNEAVKALRSALETYRYNDGAQALYEF
                     FWTEFCDWYIEATKLSFRNGTDGEKDRATSVLMNLLEESLRLLHPYLPFVTEEIYAKL
                     PLEEIAENRAKAASLNSCSNGNASKISSPSEYTGMLIAAPYPLVRAARENEAVEKSFR
                     QMQEIIRSIRALRAECGLGPELKLHVSL"
     gene            2538..3764
                     /locus_tag="PKW26_05345"
     CDS             2538..3764
                     /locus_tag="PKW26_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HOQ93024.1"
                     /translation="MIHILHTADLHLGKVIYEHSLIEDQKKVLALLKDILKKANTAKD
                     PYSALVISGDIYDRSIPNPEAISLFDSFLTEVKKENPELALFLHPGNHDSAARLSYAS
                     ELLKNQNIHIATKFSDSLKATLLKKGEERLAVFQIPFLTAGFLHGQEGSEKRRSQEEM
                     LSFVIEKIQEEKEALEIKEGLEKNSLPSLLSAHLFTLGGSESDSERTIVGTAEYVNAN
                     LFSPFTYTALGHLHKSQKVFENDSAAVYYSGAPIAYSFGEGDKSFLSVSIDTEKNKIA
                     SIKKIPIEPLRKMTQLEGSFSDFFSKDRFDAHREEYLEIKLKDANLIENPMQLLRSKF
                     PYLLSINQSYALSKQEGSSLLTERKEVLEKKNSFADILNLFIKDVDTSLDENEVEKLL
                     SLAMSIEKEKQEKEIQ"
     gene            3761..6634
                     /locus_tag="PKW26_05350"
     CDS             3761..6634
                     /locus_tag="PKW26_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ93025.1"
                     /translation="MKIEKLKLKNFGPFVGENEIDFSLLEDIFLIYGNTGSGKTTIFD
                     AVCYALYGKLPGTREKQDIRKCKSDFAKAEEDAFIELCFQVKEDSFRVKRTLPYLYET
                     KNKTIAEKPSSLVLEIKESNDTKQDARFEEDSLFQNDGNTSEWKILTDNLSEGNKKIE
                     SILGLTATEFSTIILLPQGEFAQFLKQNSQQRRESLSTLFPVEIYKNFISTVGEKTKN
                     FSAQSANLETNLLELRKVFSPEETDKKQEIFNTKITELKEERVKLNSAVSKKTEKKIH
                     LEQVINRKESYESYLEEEQKTKISFERLQKEKANIELIEKKLIAMDAALKEFIKTEKY
                     IKECEEGFDEKSKLENKQSINTHKISELQKNLRGYEKKLEDLSQDKEKLVDLLEEKNK
                     KEKELHKENEKIAEIKKRIELETTIEKLEKKTQDINKEIEILSNDTENAKNILSDFIA
                     ADEKNKEAFQASLLAEKLESGNACPVCGSKEHPQKAKPVDSLLSLEEKIQSQKNLIAV
                     AEKNLREKERIQTANTTELSLAKENLSRIENKEDLADAIKKQTTIEEEIQGLSIKIEK
                     KKNDEKEKERLSKEINTCKDTIIQAESERDALKMDAALLTQSIKIAESHVLQMEENYK
                     ELLKEIYQKYAIKKNEEASKQGLRLQYLSTQTMQLLKKDFDSQTAEVASYKDECTALE
                     KKRNTLAGQIDEAKKALEKSALFISELDEIALFETKSLSLLRELLDKTLIEIESNTAK
                     QKEIDEEIEELQAGLSKERQYAEQYAENSQKLAALQKESEVIAHLNNLLSGNNEKKTP
                     LDAWVLSLFLEEIIIYATKRLEKLSSGRYTFSLKTENAGGRGYKGLEIEIFDSFTGKN
                     RPPSTLSGGETFIASISLALALTDVVQSRSGGISIDSLFIDEGFGSLDPESLELALGI
                     IDEIRSERVVGLISHVGDLGSRINSQIIVHKSNKGSSLSLIGV"
     gene            6658..8112
                     /locus_tag="PKW26_05355"
     CDS             6658..8112
                     /locus_tag="PKW26_05355"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOQ93026.1"
                     /translation="MEKSCIRKSALFTDFYELTMAQGFWKHKMNQPAVFDMFFRRQAF
                     KAGFSVFSGIKPLLETLSEFCFSKEDLAYLSSLDFFDPAFLDYLKDFRFRGDLYAMDE
                     GSIIFPNEPIIRVHANMIEAQIIEGLVLNCINFESLIATKAARIWHSSGYGNIMEFGL
                     RRAQGSDGAMSASRAAYIGGASSTSNTLAGKRYGIPVKGTMAHSWVMSFPSEREAFET
                     YAELFPNNSIFLIDTYETLGSGIENAIFVGKKLKEKGYNFGVRLDSGDIHYLSVEVRK
                     RLDAAGLRDAFITVSNELSEEIIENLLLQKAPIDSWGVGTNMVTGGNESSFTGVYKLS
                     AHFDTEGKMHSAMKFSDNPEKTTNPGIKNVWRIYDQDGMAKADIISLIDETISTEKSQ
                     SFYHPSIDYRQFNFKPAKVELLLKQKIKNGKLLDTEKTNTAELQESRETMIRQLNSFD
                     NSYKRLLNPHVYKVSISEGLKDLKLKHIQNRLGH"
     gene            complement(8145..8867)
                     /locus_tag="PKW26_05360"
     CDS             complement(8145..8867)
                     /locus_tag="PKW26_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HOQ93027.1"
                     /translation="MKKNKVFSLIDKAVFDFSLLEDGDRILIGASGGKDSTILAEYLS
                     QRQKQKRENFSLAALHIGTDISPPLSEEIKALYKDWGIALHIQEFSVLDRVREGKKMN
                     CWWCSTQRRTALNNFALEGSFNKIALGHHLDDVLETLLMNAVNKAELSTMIPKLPYEK
                     YPVTIIRPLYYVDEKSIILRAKKEGFASFTCTCDYQAFSERKNMREKLRVLTDGSFDA
                     KMRLLSSLQNIKADYLPQKTKV"
     gene            complement(8980..10212)
                     /gene="secF"
                     /locus_tag="PKW26_05365"
     CDS             complement(8980..10212)
                     /gene="secF"
                     /locus_tag="PKW26_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOQ93028.1"
                     /translation="MKKVIRFSRFFVPALILSAAILISGMYRLFTVGINFGIDFKPGL
                     MQDVKIADTVFTLSYTGTSSVSVDTSAQGFNLVVSGLGSEKTTHSFGYLQYKTAGEML
                     AALNAVPGIEAKLEVPENTPVSNLFTSSSLSRTLSASKTPVYIADTSQVHSIENVRSA
                     LQSIPDIAVKSVGDARENSFQIRIGDDGSDPEMSKKTQELIYNLLCNEFGSDKVAVIK
                     TDFVASQFSKTLVTQSIILVLATLFLIWIYATVRFKWDFALGAVLAIIHDTLVMVAVM
                     SWTQMEFNSITIAAILTIVGYSINDTVVVLDRVRENVRLVKTNKFVDIINLSQTESLS
                     RTIITTATTMLAVVSLYIFTSGSMKDFAFALLVGMVSGVYSTIFISSAFISLTRKNWT
                     PSEDERKTQVKEFSEVLV"
     gene            complement(10209..11951)
                     /gene="secD"
                     /locus_tag="PKW26_05370"
     CDS             complement(10209..11951)
                     /gene="secD"
                     /locus_tag="PKW26_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOQ93029.1"
                     /translation="MSKRGRFLLILGIIACCIFFLWPTINWYGLTPKEDQVLALGSLE
                     TIKDFAGYKASEDVKTLKALAKENPGAAIPLEHAYLIKEAKKNYKLLKKNFPKEPSIR
                     DLLAGFQNELELMNAIETMYREQILKNKRYYNNSVKLGLDLSGGMSVIVKADLEAAAK
                     DLGKMTSDELATFNDNAMTQAIETLRGRIDKFGLSEPVIRRQGENRIYIEIPGAAEVD
                     SINSIIMGKGILNFRLVDSEATDAFNAHYAAHPAETFTARGELLDLSIIPEDTEVLGL
                     YTKDEYDLDERIGYLVVKKEIVLDGKHIKSADIGSNQYDGRPQVHFTLDSDGAVIFRD
                     FTTANRDRYLAIVSDDKIKSYAKINEPIPNGQVAISGFGLDEANNLKKILQTAWLNVP
                     LSVESQQVVGASLGETAIKEGFLALVFGLLSVIVFMLLYYRGAGVNAVVAQVLNMFLL
                     ISILSAFNLTLTLPSIAGMILTIGMAVDANVIIFERIKEELRLGKKRAAAIEAGFDRA
                     FWAIMDSNITTFIAAIFLSQLGTGAIQGFAVSLAIGVISSVFTALFVSRLIFDFGTES
                     LKTEKITISWRKIK"
     gene            complement(11969..12358)
                     /gene="yajC"
                     /locus_tag="PKW26_05375"
     CDS             complement(11969..12358)
                     /gene="yajC"
                     /locus_tag="PKW26_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOQ93030.1"
                     /translation="MFTLLQTASAGGSGSLLMSMAPIAIIFLIFYFLMIRPQNKKQKE
                     TEKMIAALKKGDKIVTIGGIHGVVSTTKEQTVIVKVDDNAKMEFSRSAIAQVITDAKD
                     KTEKLEKKSKSDKKTQSEDSIPAKEEK"
     gene            complement(12457..12615)
                     /locus_tag="PKW26_05380"
     CDS             complement(12457..12615)
                     /locus_tag="PKW26_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOQ93031.1"
                     /translation="MAKYVCTLCGWVYDPAEGDPDGGVAPGTAFEDIPEDWVCPVCGA
                     TKSAFEKV"
BASE COUNT         4340 a         2661 c         2352 g         3584 t
ORIGIN      
        1 agagaaacat gtagttttaa ctcagggcct aaaccgcatt cagctcgcag ggcacgaata
       61 gagcgaatta tctcctgcat ctgtctaaat gatttttcta cagcttcatt ttctcgcgcc
      121 gctcttacaa ggggataggg agctgcaatg agcatgcctg tgtattcgct tggcgatgag
      181 attttcgacg cattgccatt gctacaagaa ttaagtgaag ctgccttcgc ccggttttca
      241 gcaatttcct ctaagggaag ttttgcatag atttcttctg taacaaaggg tagataggga
      301 tgcaagagtc gaaggctttc ttcaaggaga ttcatcaaaa ccgaggttgc cctatccttc
      361 tcgccatctg tgccatttct aaacgaaagt tttgttgctt caatatacca gtcgcaaaac
      421 tctgtccaaa aaaattcgta caaagcttgt gccccatcat tatagcgata tgtttcaagg
      481 gcactacgaa gcgcttttac cgcctcattg agacgagcat atatccattt atcgaggtct
      541 gttaaatcgg aatcttggat aggaacgaga tttctctctt ctagattacc gagcaagtag
      601 cgacttgcat tccatatttt atttgcaaag cgacttccca acttaaaaga ttctttatcg
      661 attaaaatat cttgtccctg agcgcacata aagccaaggg taaacttcat agcatcggag
      721 ccaaactctt cggtaatttc tagggggtca agcccgttac caagagattt actcatcttg
      781 cgaccttgtt tatcgcgtac gagtccatga atataaatat ctttaaaagg cacctttccg
      841 gtaaattcaa gacctgccat tatcatgcgg cttacccaaa aaaagataat atcataggcg
      901 gtaacgaggg ctgttgtcgg ataaaaggtt ttaaaatcgg aggctaggtc ttcgaggttt
      961 ttcccgtttt ctggccaacc gagggttgaa aaaggccaga gccagctaga aaaccaagtg
     1021 tcaagaacgt ctgcatcttg atgaatctct ttgcttttgc actttgtaca ttcgcttagg
     1081 tcaacacggc taaccatcat ttcaccacag ttatcacaat accaaacggg aatgcgatgt
     1141 ccccaccata attgacggct tatgcaccaa tcgcgaatat tttccatcca atgcgaataa
     1201 gtgttctccc atttttttgg ataaaacaca atgtcgccgt ccttccaagc tttcaaagcc
     1261 ttatcggcaa ggggcttcat tttgacaaac cactgatcgc tcagataggg ctcgacaacg
     1321 gtgtcgcatc gataacaatg cccgacggcg tgagatattg gtttttcttc cttatataaa
     1381 cccagttcta ctaaatcttc taagactttt tttcttgcct cttttgagga aagtccttga
     1441 tagattttag ggacggcgtc gtttaagctg ccgtcgggat tgagaatatt tataatttct
     1501 agattatgtc gttgaccaag ttcccagtca tttgggtcgt gagctggcgt tattttaacc
     1561 acacctgttc caaaagaagg gtcgacatag ctgtcggcaa caacgggaat acttcgccca
     1621 gttaagggca atagcacttt tttaccaatg agagcggtat agcgtgggtc atcgggatgc
     1681 accgcaacgg cggtatcgcc aaggagggtt tctggtcgcg ttgtcgcgat ttcaatttta
     1741 ttgccaccag gaaactcagt tccgtcttcg agttttacag caggaagaga agcaccttca
     1801 atttcataat agaggtgata catcatccct tgtttgtctg tatgttcaac ttcgtcgtca
     1861 gcgagggcgg ttccacaaga ggtacaccag tttaccagat aatttccctt gtatatcaag
     1921 cctctttcaa aaaggcttac aaacacttcg cgaacagcac gagataagcc ctcgtccatg
     1981 gtaaatcgct cgcgactcca atcgacagag gctcctattt ttttaagctg ctttgtaata
     2041 atttcatggt gctcttcttt cacttcccat gtttttttta caaaggcttc acgaccaagg
     2101 tcatggcgag aaagcccgtc tttttttagg cgacgctcta ccacattttg agttgcaata
     2161 ccagcatgat ctgttcctgg aagccagagg gtatttttcc ctgtcattcg atgatagcga
     2221 acaataatat cttgcaatgc gttatttaaa ccatgcccca tatgaagcac cccggttaca
     2281 ttcggaggag gaataacaac agtgaaactt tctgcatttg caggagcgga taggctctct
     2341 gatgctttat ttgcagaata caaggctgag tctgtatcag tttttggctt aaaatagcct
     2401 tttttttccc agtctgcgta aattctatct tcaaagtctt ttggattata tgccttttct
     2461 aattctatac ctttcatagc acaaataata ctcttttttc gtttgagttt caatgcttaa
     2521 aatgctatac tttttctatg atacatattt tacataccgc agacctccat cttggaaaag
     2581 ttatatacga gcattctctc atagaagacc aaaaaaaagt cttagccctt ctaaaagaca
     2641 ttctaaaaaa agccaataca gcaaaggatc cctattccgc cctcgtcatt tctggcgata
     2701 tttacgaccg ctccatcccc aatcccgaag caatcagtct ttttgatagc tttttaaccg
     2761 aagtaaaaaa agaaaaccca gaacttgctc tttttcttca tcccggaaac cacgactccg
     2821 cagctcgcct ttcttatgcc tctgaacttt taaaaaatca aaatatccac atcgcaacaa
     2881 agtttagcga ttccctaaaa gcgactctct taaaaaaagg agaagaaagg ctcgctgtct
     2941 ttcaaattcc ctttttaaca gcaggttttt tacatggaca agaaggctct gaaaaacgac
     3001 gtagccaaga ggaaatgctt tcctttgtta tcgaaaaaat ccaagaagaa aaggaagcac
     3061 ttgaaataaa agaaggctta gaaaaaaact ctttaccaag ccttttgagc gcccatcttt
     3121 ttaccctcgg tggaagtgaa agcgattcag agcgcacaat agtgggaaca gccgagtatg
     3181 taaacgcaaa tcttttttct cccttcacct ataccgccct cggacactta cacaaaagcc
     3241 aaaaagtttt tgaaaacgat tctgctgccg tgtattattc tggcgccccc atcgcctact
     3301 cctttgggga aggagataaa tcttttttgt ccgtcagcat cgatacagaa aaaaataaga
     3361 tagcatcgat aaaaaaaata cccatagaac ctttgcgaaa aatgacgcaa cttgaaggaa
     3421 gcttttcaga cttttttagc aaagacagat ttgacgccca tcgagaagaa tatcttgaga
     3481 taaaactgaa ggatgcaaat cttatagaaa accccatgca attattgcga tcaaaatttc
     3541 cctatctact ttctataaac caaagttacg cactttctaa acaagaaggt tcatcgcttt
     3601 taacagaacg aaaagaagtc ttagagaaaa agaattcctt tgcagatatt ttaaatcttt
     3661 ttataaaaga tgtagataca agccttgatg aaaatgaagt tgaaaaactt ctgtctttgg
     3721 ccatgagtat agaaaaagaa aaacaagaaa aggaaatcca atgaagatag aaaaactcaa
     3781 actcaaaaac tttggtccct ttgttggaga aaacgaaatt gactttagcc tccttgaaga
     3841 tattttcttg atttacggaa acactggctc agggaaaaca actatcttcg atgcggtatg
     3901 ctatgccctc tacggaaaac ttcctggcac aagagaaaaa caagatatac gaaaatgcaa
     3961 aagcgacttt gcaaaagcag aagaagatgc ctttatagaa ctctgctttc aagtaaaaga
     4021 agatagcttt cgagtaaaaa ggacgctccc ctacctctat gaaacaaaaa ataaaactat
     4081 tgcagaaaag ccatcttctc tcgtccttga aataaaagaa tcgaatgata caaaacaaga
     4141 tgcccgtttc gaagaagata gcttgtttca aaacgatgga aatactagcg aatggaaaat
     4201 tctaactgat aatctgagtg aaggaaataa aaaaatagaa tctatcctcg gtttaactgc
     4261 aacagaattc agtaccatca ttttacttcc gcagggagaa tttgcccagt ttctcaagca
     4321 aaactcacaa caaagacggg agtctttaag cacgcttttt cctgtagaaa tctacaaaaa
     4381 ctttatatcc acagtgggag aaaaaacaaa aaactttagc gctcagagtg caaatctcga
     4441 aacaaatctt ttagaattgc gaaaagtatt ttcaccagaa gagacggata aaaaacaaga
     4501 aatcttcaat acaaaaatta cagaactaaa ggaagaaaga gtaaaactca attctgcagt
     4561 atctaaaaaa acagaaaaaa aaatacacct cgagcaagtc ataaacagaa aagaaagtta
     4621 tgagtcttat ctggaagaag aacagaagac aaagatttcc tttgaaagac ttcaaaaaga
     4681 aaaggcaaat attgaactaa ttgaaaaaaa actcatagca atggatgccg ctttaaaaga
     4741 atttattaag acagaaaaat atattaagga atgtgaagaa gggtttgatg aaaaatccaa
     4801 acttgaaaac aaacaaagta tcaatacaca taaaatctca gagctgcaga aaaatctcag
     4861 aggctatgaa aaaaaacttg aagacctaag tcaagacaaa gaaaaactcg tagaccttct
     4921 tgaagaaaaa aataaaaagg aaaaagaact acacaaagag aatgaaaaga tagccgagat
     4981 aaaaaaaaga atagaactag aaacaacaat cgaaaaactc gaaaaaaaaa cacaagatat
     5041 aaataaggaa atagaaatct tatccaatga taccgaaaat gcaaaaaata ttttgtctga
     5101 ttttattgca gcggatgaaa aaaacaaaga agcctttcaa gcttctctac ttgcagaaaa
     5161 attggaaagt ggaaacgctt gtcccgtctg tggctcaaaa gagcatccac aaaaagcaaa
     5221 acctgtggac agccttttat ctcttgaaga aaaaatacaa agtcaaaaaa atcttatagc
     5281 tgtggcagaa aaaaacctga gagaaaaaga aaggattcaa acagcaaata ctaccgagct
     5341 atcacttgcg aaagaaaatc tatctcgtat agaaaataaa gaagatttag ctgatgcaat
     5401 aaaaaaacaa acaacaatag aagaagaaat acaagggctc agtataaaaa tagaaaagaa
     5461 aaaaaatgat gaaaaagaaa aggagcgact cagtaaggaa ataaacacct gcaaagatac
     5521 tataatacaa gcagaaagcg aaagagatgc gcttaaaatg gatgcggcac ttttgacaca
     5581 gagtatcaaa atcgcagaaa gccatgtcct ccagatggaa gaaaattata aagagcttct
     5641 aaaagaaatc taccaaaagt atgcaatcaa aaaaaacgaa gaagcttcca aacaaggact
     5701 tagactacaa tacctttcaa cacagaccat gcagcttctg aaaaaagact ttgactctca
     5761 aacagctgaa gtagctagct ataaagacga atgcacagcc ctagaaaaaa aacgcaatac
     5821 tcttgccgga caaattgatg aagcaaaaaa agcgctagaa aaatcagcac tttttatatc
     5881 cgagctagat gaaatagcac tatttgaaac aaagtctctc tccttgctta gagaacttct
     5941 agacaaaacc cttattgaaa tagaaagcaa tactgcgaaa caaaaggaaa tagacgaaga
     6001 gatagaagaa ttacaagcag ggctatcgaa agaaaggcaa tatgcagaac aatacgctga
     6061 gaacagccaa aaacttgcgg ccttacaaaa agaatctgag gtgatagctc atcttaacaa
     6121 tttgctttct ggaaataacg aaaagaaaac accgctcgac gcctgggttt taagcttgtt
     6181 tttagaagaa attattatct atgcaacaaa acgtttagaa aaactttcaa gtggtcggta
     6241 taccttttcc cttaaaactg aaaatgcagg aggcagagga tacaagggat tggaaataga
     6301 aatctttgac tccttcacag gaaaaaatcg ccctccctct accctttccg gcggtgaaac
     6361 ctttatcgct tcgattagtc ttgcactcgc actcaccgat gttgtacaat caaggtctgg
     6421 aggcatcagt atcgattcac tttttatcga tgaaggattt ggctcccttg acccagaaag
     6481 cttagaactc gctcttggca tcatcgatga aattcgctca gaaagagtag taggtttaat
     6541 ctcccacgtc ggagatcttg gttcacgtat taattcgcaa attatcgtac acaaatcaaa
     6601 taaaggtagt agcctaagcc tcatcggcgt ttaggctcac aaagaaagga agaaaacatg
     6661 gaaaaatcat gtataagaaa atcggcactt tttacagact tttatgaact taccatggca
     6721 caaggctttt ggaagcataa gatgaatcaa ccagctgtct tcgatatgtt ttttagaaga
     6781 caggcattta aagcaggctt ttctgttttt tcaggaatca agccactctt ggaaacactg
     6841 agcgaatttt gcttttcaaa ggaagacctc gcctatcttt catccctcga tttttttgac
     6901 ccggcttttt tagactacct caaagatttt cgtttccgtg gggacctcta tgcaatggac
     6961 gaaggctcta ttatttttcc aaacgagcca attattcgcg ttcatgcaaa catgatcgaa
     7021 gcacagataa tcgaaggact tgttttaaat tgcataaatt ttgaaagcct catagcaacc
     7081 aaagccgccc gtatctggca ttcaagcggc tatggaaaca tcatggagtt tggacttcgt
     7141 cgcgcacaag gctccgatgg cgctatgagt gcatcacggg ctgcctatat cggtggagca
     7201 tcttctacaa gcaataccct cgcgggaaaa cgctacggca tccccgtaaa aggcacaatg
     7261 gcacattcat gggttatgtc ttttcccagt gaacgagaag cctttgaaac atacgcagag
     7321 ctttttccaa acaactctat ctttctcatc gacacctatg aaacactcgg ctcaggcata
     7381 gaaaacgcta tctttgtggg aaaaaaacta aaagaaaaag gctataattt tggcgtacgc
     7441 ctcgactctg gagacataca ttatctttca gtagaagtgc gaaaacgcct cgatgcggca
     7501 ggtcttcgcg atgcctttat cactgtttca aacgaactat cggaagagat tatcgaaaac
     7561 ttgctgctac aaaaagcacc catcgatagc tggggcgttg gcaccaatat ggttaccggc
     7621 ggaaatgaat cttcctttac tggtgtgtat aaattatctg ctcattttga tacagaagga
     7681 aaaatgcaca gcgctatgaa gttttcagat aatccagaaa aaacaacaaa ccccggcata
     7741 aaaaatgtct ggcgcatcta cgaccaagac ggtatggcaa aggctgatat tatttccctc
     7801 attgacgaaa caatttctac tgaaaaaagt cagtcttttt atcatccatc gattgattat
     7861 agacagttta attttaagcc tgcgaaggtt gaacttttac taaaacaaaa aataaaaaac
     7921 ggaaagctct tagatacaga aaaaacaaat acagctgaac tccaagaaag tcgcgaaaca
     7981 atgattcggc aactaaatag ctttgacaat tcctataagc gattgttaaa tccccatgtg
     8041 tataaggttt ctataagcga gggcttaaag gacttaaagc tcaaacatat tcaaaaccga
     8101 ctcggacatt aaaaaagact gcgatataaa agagaagctc ctcatcagac ttttgttttt
     8161 tgagggagat aatcggcttt tatattttgc agggaagata ataaacgcat ctttgcatca
     8221 aagcttccat cagtaagaac tcgtaatttt tctctcatat tttttcgctc agaaaaagct
     8281 tgataatcgc aagtacaagt aaagcttgca aagccttctt tttttgcgcg aagtataata
     8341 gatttttcat ctacataata aagcgggcga attatcgtta ccggatattt ttcatagggc
     8401 agtttaggaa tcatcgtaga aagctcagcc ttgttcaccg cattcatcaa tagggtttcc
     8461 aaaacatcat ccagatggtg gccgagggca attttgttaa aacttccttc cagcgcaaaa
     8521 ttgttcagcg cagttcttct ttgtgtagaa caccaccagc agttcatttt ctttccttca
     8581 cgcacacgat ccaaaacaga aaactcttgg atatgtaggg ctatacccca gtccttgtac
     8641 aaagctttta tttcttcgct caatggtgga cttatatctg ttcctatgtg aagcgctgca
     8701 aggctaaaat tttctctctt ttgtttttgc ctttgactta aatattcagc aagtatggta
     8761 gaatctttac cacccgatgc accgattaaa atacggtcgc catcttctaa aagagaaaaa
     8821 tcaaaaacag ccttatcgat gagggaaaac actttgtttt ttttcatagg ctactataac
     8881 atatttccaa taaaataata caagaaaaca tacagagtgc aaaaaaaact gccctcttac
     8941 aaagtgtaaa aaggcagctc tctttagtgt cgatataaat tatacaagaa cttctgaaaa
     9001 ttcttttacc tgtgttttcc gctcgtcttc actcggggtc cagttttttc tcgtaagaga
     9061 aataaaggcg ctcgaaataa agatagtaga atacacacct gaaaccattc ctacaaggag
     9121 ggcaaaggca aagtctttca tagaaccact tgtaaaaata tacaaggaaa caacggcaag
     9181 cattgtggta gcagtagtga ttattgttcg gcttaaactt tcagtttgtg aaagattgat
     9241 aatatcaaca aacttatttg tttttactaa acggacattt tcacgaacac ggtcaagaac
     9301 aacaaccgta tcgtttatag aataaccgac gatggtaaga attgccgcaa ttgttataga
     9361 gttgaattcc atttgcgtcc agctcatcac agccaccatt acaagcgtat cgtgaataat
     9421 tgcaagaaca gcaccaaggg caaagtccca cttaaagcga acagtcgcat aaatccaaat
     9481 caaaaacaag gtcgccaaaa ccaaaataat tgattgcgta acaagggttt tagaaaactg
     9541 tgaagccaca aagtctgttt ttattaccgc aaccttatcg gagccaaatt cattgcaaag
     9601 caagttataa ataagctctt gtgttttttt actcatttct gggtcagagc catcatcgcc
     9661 aatacgaatt tgaaaactat tctcccgcgc atccccaacc gacttcactg cgatgtcagg
     9721 gatactttgt aaggcagagc gaacattttc aatgctatgc acctgacttg tatctgcaat
     9781 atacaccggt gttttgctcg cagaaagcgt tcttgaaaga gaagacgagg taaacaggtt
     9841 tgaaacaggc gtattttctg gaacttccaa ttttgcttca atgccaggaa cagcattcaa
     9901 ggcagcaagc atttctcctg cggttttgta ttgcaagtag ccaaaactat gcgttgtttt
     9961 ttcagaacca agtccactca ccacgagatt aaaaccttgt gcactggtat ctacagaaac
    10021 agaagaagtt cctgtgtacg acaaagtaaa tacagtgtct gcaattttta cgtcctgcat
    10081 taagccaggc ttaaaatcga taccaaagtt tatacccacc gtaaataaac gatacattcc
    10141 gctaataagt attgcagcac tcaggataag agctggaaca aaaaagcgac taaaacgaat
    10201 tacttttttc attttatctt cctccagctg atagtaattt tttctgtttt aagagactcc
    10261 gtaccaaagt caaaaataag ccgtgataca aaaagcgcag taaagacact ggaaataaca
    10321 ccaatcgcca agctcacagc aaaaccttga attgcacctg ttcccaattg tgaaaggaag
    10381 atagctgcga taaaggttgt tatatttgag tccataattg cccaaaaagc cctgtcaaaa
    10441 cctgcttcaa ttgcagcggc tctcttttta cctaaacgaa gttcctcttt tattctttca
    10501 aaaataataa cgttcgcatc aacagccata ccaatcgtta aaatcatacc ggcaatgctc
    10561 ggcaaggtaa gcgttaagtt aaaggcagat aaaatactaa taagcaaaaa catattgagc
    10621 acttgtgcta caactgcatt tacaccagct ccacgataat acaaaagcat aaagactata
    10681 acggataata gtccaaaaac caaagctaaa aagccttctt taattgcggt ttctccaaga
    10741 gaagcaccaa caacttgttg actttcaact gaaagcggca catttaacca agcagtttgt
    10801 aaaatctttt tgaggttatt tgcttcatct aagccaaaac cagagattgc aacctggcca
    10861 tttggaattg gctcgtttat ttttgcataa gatttaattt tatcatctga aacaatggcg
    10921 agatagcgat cgcgatttgc agtcgtaaaa tcacggaaaa taacagcacc gtctgaatca
    10981 agcgtaaaat gcacctgcgg tcgcccgtcg tattgattcg agccaatatc ggcagattta
    11041 atatgcttac cgtcaaggac tatttctttt tttaccacaa gatagccaat gcgttcatca
    11101 agatcgtatt cgtccttagt atacaaaccg agaacctcgg tatcctctgg gataatagaa
    11161 agatcgagta attcgccacg agcggtgaag gtttctgcag gatgcgctgc ataatgtgca
    11221 ttaaatgcat cagttgcttc agagtctaca aggcgaaaat ttaaaattcc cttacccatg
    11281 attatggagt taatagaatc aacttcagca gcaccgggaa tttcgatata aatgcgattt
    11341 tcaccctgcc tacgaataac aggctcggaa aggccaaatt tatcgattct gccgcgaagg
    11401 gtttcgattg cctgggtcat cgcattgtcg ttaaaagttg caagctcatc ggaagtcatt
    11461 ttacctaagt cctttgcagc tgcttctaag tccgccttga caataacact cataccacca
    11521 gacaagtcga gtccgagttt tacagagtta ttgtaatagc gcttattttt taaaatttgt
    11581 tcgcgataca ttgtttcgat tgcattcatc aattccagct cattttgaaa accagctaaa
    11641 aggtcgcgaa tactcggctc ttttggaaag ttttttttca aaagcttata attttttttc
    11701 gcttctttta taagataggc atgttctaat ggaatcgctg caccaggatt ttcttttgct
    11761 aaagccttaa gtgttttaac atcttcagat gccttataac cagcaaaatc cttaattgtt
    11821 tctaaagaac ctaatgcaag tacttgatct tctttcggtg taagcccata ccaattgata
    11881 gttggccata aaaagaaaat acaacaggca ataattccca gaattagaag aaaacgtcct
    11941 cgtttactca ttttaattct ccagtttatt atttttcttc tttagcgggg attgaatctt
    12001 cgctttgagt ttttttatct gattttgatt ttttttcaag tttttctgtt ttgtcttttg
    12061 cgtctgttat aacctgagcg atagcagagc gagaaaactc catctttgca ttgtcatcaa
    12121 ctttaacaat aacagtttgt tcttttgtag tactcactac cccatgaata ccaccgatgg
    12181 taacaatttt gtcgcctttt tttaaagcgg caatcatttt ttctgtttct ttttgctttt
    12241 tattttgagg gcgaatcatc aaaaaataaa atataagaaa gatgattgca attggggcca
    12301 ttgacatgag caaagaaccc gaaccacctg ctgaggcggt ctgtagtaat gtgaacataa
    12361 tttttccttt attaatttaa acaaattttt gtctaaatag aatatcacat tactaaacag
    12421 accgtcaagg gaaaagcttg atcgctattt taaaagttag actttctcaa acgcactttt
    12481 cgttgcaccg cagacgggac aaacccaatc ctctggaata tcttcaaaag ctgttcctgg
    12541 agcaactcca ccatctggat caccttcagc tggatcatac acccacccac ataatgtaca
    12601 tacatacttc gccataattc ctcctaaaaa aaagcaatga gaaaatttca attttcgaaa
    12661 ttgctttttt tcagcacttt cgcaacagag tgagaaagtg cacgtatata taaagtttgc
    12721 aacgcaaact tttaaatgaa cagtgaggca ccatttttgc cgaactgttc attatcctcc
    12781 tttaaactcg ccatggatgg cgagatgcaa caaatttttg acatgttttg caacgcaaaa
    12841 ctgtctgaat attttttaca tggatgtaaa aaatattcta gcaaactttt aaatgaacag
    12901 tataacgttt tgccgaactg ttcattatcc tcctaaa
//
LOCUS       DAOSSW010000049        12871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_4884_length_12871_cov_6.824048, whole genome shotgun sequence.
ACCESSION   DAOSSW010000049 DAOSSW010000000
VERSION     DAOSSW010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 12871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12871
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4884_length_12871_cov_6.824048"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PKW26_05385"
     CDS             <1..396
                     /locus_tag="PKW26_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOQ93032.1"
                     /translation="QNVRILEHDALEVLEYMIPDNSISAFHVFFPDPWPKKKHHKRRF
                     MLRPNTGLLAKKLKKDACIYMATDWEDYAYHALEELTATEGLKNKYEDLKNSLLGFAP
                     KQDWRPETGFEKKGKNANRRIFDLVFVKS"
     gene            407..2395
                     /gene="ligA"
                     /locus_tag="PKW26_05390"
     CDS             407..2395
                     /gene="ligA"
                     /locus_tag="PKW26_05390"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOQ93033.1"
                     /translation="MKKEYSVEELVSLIEGYQASYYGGEAEISDEAFDQLWDLLKEKD
                     PDNPLLQKIGSDIGEAEKTKTGGVFQKVRHLIPMGSQEKAANPEEFRAWADKMSFKNF
                     LVEYKLDGASLELQFEKGLFTRAVTRGDGEIGDDITKNVLKMQGLVKSLKDASGNRLD
                     ISGGVRAEVIMKKEIHKTYYPDKANCRNAANGLMKRKDGSGSEHLHILCYDAFFSGQE
                     ALYTNEKEKVLWIEKQGFEIVPFKLCQGANEVIDFRAQVMEEREGLDYDIDGLVVKGL
                     EIDLADAERRRPEKQIAFKFTLEEAISIVKNIIWSESGVTYTPIAEFEPVFLAGTTVQ
                     RASLVNPNTIRNLGIKIGSHVVVTKRGEIIPKIEYVLEGYKHSYEKNENSLGSLFDEE
                     NNIFPTKCSACNTKLFDEGTRLFCPNKACPKRIHHRLEKWVASLDIRDLGIGLIEKLF
                     LSGKLSSIKDFYSLTEDDLRPFFLNEESLEKEKESLGAKKVYQSIQDRKTLSLSTFIA
                     GFDIEGFGETQIENLIQAGYKKLEDFFSASVDEIASVHGFGEISARNLVEGLAEHKEE
                     MLFLISNKILVLREETEAALSGKSFCFTGELYTMKRSEAEALVKKNGGAVKSSVVKDL
                     SFLVTNNIESGSSKNKKAGELGIPIIDEKEFLRLIGHE"
     gene            2388..2993
                     /locus_tag="PKW26_05395"
     CDS             2388..2993
                     /locus_tag="PKW26_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93034.1"
                     /translation="MNRTFFTRILFYFFILIVFIFSVNTVHLLLIIKNIQRLPELPKP
                     YEMMRFVINSYGDSSVNARFWFYDLQGNELASLERSWPADYLKVHFTYAQYNKKMLVY
                     PTTIQNIQVNKKRQAHGTDLRPFVQIKTYTRAPRSTDENTASEKALRSLEAFILDKNI
                     FKKTFFSFRPFFLEDLSLSLERAERGRIYSLYFENGRVYVQ"
     gene            complement(2996..3964)
                     /locus_tag="PKW26_05400"
     CDS             complement(2996..3964)
                     /locus_tag="PKW26_05400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ93035.1"
                     /translation="MTIQKKLFLFFMLTLFTSSPFFSNSKTFRFGSNIITSIFDENLY
                     PGDPVFLKLLVESPFIKNEINARVEIIENKTGKRIRSAKMYNLEEGNINPTTKILFVG
                     LPLSTYLSPGSYTMQIDFQSNALNERLLIPFSLKEKIFVQETIPLDPVNTAIKTDDSP
                     QRMAQIDNLNAVLNTINLEAKKTIEAFMQPVHSTRRTSFFGDRRTFAYSNGKSETNLH
                     FGIDFGVPIGTEIFACASGKVLMSEMRNSTGWTIVIEHFPGLYSLYYHLDKMLVKKDD
                     FVQKGDLIGLSGKTGLATGPHLHWEMRLLAEAVNPDYFVERFDLFK"
     gene            complement(3981..6122)
                     /gene="recJ"
                     /locus_tag="PKW26_05405"
     CDS             complement(3981..6122)
                     /gene="recJ"
                     /locus_tag="PKW26_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOQ93036.1"
                     /translation="MAIWKKEAISKEIIKALDTRYSCGPLVSTILARRGITEGEDILY
                     FLEDDLRYQHNPFLFNEMEDAVDRIMDAKAEGEKVLIFGDRDVDGITSTVLLYECLKD
                     LGIDVSYRLPKDDEPYGLNIQAIDDFAENYGSLIITVDCGISNYDEIQYAHEKGISVI
                     ITDHHNPPEKLPEDCIIINPKMEGEDYPFEHISGCAVAYKLATALRFAQSDAYKQEIC
                     LLHVRPLKDAYQIECIKIQNMCEKERLSETIVPGLISISKTRLPEFLQGQQIFVWDEA
                     IEKKLLKDAFGAGIEFNLYDIQNDIASLIPSVKDLSLLRLKQFSKIARYHPEKNTEID
                     VFFNIFITFMQKLGDSKTKNNNDLGLNKDESDLQLLAISTIADIMPLKNENRLFVKQA
                     LKTINEGRIRPGLQELMARQNLLGKKITSTDIAWSITPALNATGRLGSPEISLELLLE
                     TEQKKRNEICESIIEINNKRKELGNEAWGYAENAIFKSLDDYHQNIVVIYDERIHRGV
                     TGIIAARVSQLSNRPAFILTSLEDSIIGSVRSNKNLEVMPILNQCSDLFTNFGGHAFA
                     AGFGLEKEKLPELLNRLKTISRNLEISDVEESLAIDAQLQEKHLTQEILKTIDLFEPY
                     GEENPPLLFASDKLKILNADILGKVEPFHLKLTLAVDKTKWPALFWRESKRLKRDFDT
                     GDRINLAYTISRNTFNGMESLQMIVSDIQKL"
     gene            6216..>7318
                     /locus_tag="PKW26_05410"
     CDS             6216..>7318
                     /locus_tag="PKW26_05410"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOQ93037.1"
                     /translation="MNEKMLHINDVYNGFTVLDISPILDYKSEGIWLRHNETGLEIFH
                     MYNDDSENLFSFAFKTIPSDSTGLAHIMEHSVLCGSKNYPLKDPFIRMANQSVKTFLN
                     AMTFPDKTVYPASSTVEADYFNLMAVYADAVFFPLLKKEIFMQEAHRLSLGEDGAVSI
                     QGVVFNEMMGNYSSFDSMVQEKIVQTILPGTTYAFDSGGDPREIPLLSYERFLDFHKT
                     FYQPSNCRLFLYGNIPTTKQIDFLNEKVLSQFKTATNPFSQMFLSKQDSDFCAQTQVR
                     AFTAPQNHVCYGPSVKGAGSENGDSLLLTWLLGFTSDPQSYMESLFLAELLLGHDGAP
                     LAKALIESGLGEDLEPASGIETDLRYILFTAGMR"
     assembly_gap    7319..7418
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7419..9224
                     /locus_tag="PKW26_05415"
     CDS             <7419..9224
                     /locus_tag="PKW26_05415"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93038.1"
                     /translation="TFAAIKKRIYEDDSYLAKLLEKYFIRNQHRVRLMMKSSPDFEKE
                     LQEKVALETKRLFENYVELYVEKKGISKTETQSVKEIDEKIRSIIQKENEDLASFQEA
                     EEDGSLLSLLPHLKPKDLDPSIDIIETKRREIQNIPTFLHEQEVNGILYFTLGFPCDL
                     LEAKDYLLLPFFCMAASNSGFDSLDWAEASSKLAQATGGFSASPLSSPIDEELKRSLG
                     IVDENGKTILLEEDATERLYKADPSLGRSWIFFKMKTLEEKSLKALELVFSCIKNVDF
                     SDKKRIEDLLIEYKNGLVSSIIPGGTAYAVSRANCFFSRETAIDEIWSGLTQIAFIKE
                     LSKKNIDEVIQSLLRIKKQLLESGAIINIIAEKQSMPRLISEVEKGMQDFFPEVQAPK
                     KPRLQKDEAFFELLRFSPNTDDKTEVFILPTQVGFAASCFSMRGNEKEQVAISVLAHY
                     LTNTAFWAQIRTMGGAYGAQAFYNSLEAVFLCATFRDPNPERSLAVFVDECKKLSSLS
                     FDTNLIEKLITGSYSKEIQPKTPSIKGSLGFIRSLYGINDEKRQRRMQYLLEMDEETL
                     KKVSKDIAHSLFDMYSAVINQKALSKTGKNIYFDV"
     gene            9290..9502
                     /locus_tag="PKW26_05420"
     CDS             9290..9502
                     /locus_tag="PKW26_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93039.1"
                     /translation="MNKKLEECVKKLRNLVSDVQGAPYPGNVNEELYYIWYEHAQKNA
                     MECFEFLDEYFPREKEEIKKDLLKVM"
     gene            9566..>10053
                     /locus_tag="PKW26_05425"
     CDS             9566..>10053
                     /locus_tag="PKW26_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93040.1"
                     /translation="MMQTRCGFIHKPFDLKSLGKSKQFEILRYFIPVKHNKTGHLDVF
                     FIELFSINPSLYTRQLQFASIAEKKIKAERSSFILVRAGLIAHGEKCKHIFFTSRDIR
                     YDKKNKRMQIGSSFFASDAISGSIEQESQPIEWKITFTSKEFLSYFLSHRFYFSKKDR
                     LKT"
     assembly_gap    10054..10153
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10154..10333
                     /locus_tag="PKW26_05430"
     CDS             <10154..10333
                     /locus_tag="PKW26_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93041.1"
                     /translation="GYSFESIQRLDTNHLFSLGAKGYGELRLYKKKKKNLEIIEHVRI
                     ENCLCEQALNFPPNI"
     gene            10374..>12871
                     /gene="clpB"
                     /locus_tag="PKW26_05435"
     CDS             10374..>12871
                     /gene="clpB"
                     /locus_tag="PKW26_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOQ93042.1"
                     /translation="MNYDRLTIKAQEAIQVASTIAQQEDHSEIGSEHLLFALLDQKDG
                     IIPPLVERVGVQVSTLLNDVEELLDVYPRVTGNVQVALSNDVQKILAKAEKETENLKD
                     DYVSTEHIFLALESSHSKVTQVLHRYGITRHAILQALSSIRGNQRITSQEPEATMQSL
                     EKYCKDLTQLARQEKIDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIAEGL
                     ARRMVSGDVPDSLKNKRLLALDLGALVAGAKFRGEFEERLKAVINEVQKSDGQIILFI
                     DELHTLVGAGASEGSMDASNLLKPALARGELRAIGATTLDEYRKYIEKDAALERRFQQ
                     VYCAEPSVEDTIAILRGLKEKYEVHHGVRIKDEALVAASLLSNRYITNRFLPDKAIDL
                     VDEAASKLKMEIESQPTELDQVERRIMQLTIEKVSLAKEEDASSKERLAKLEKELADI
                     SEKRDAMKAQWENEKKRIDERRHVKEELEQLRNDEVRFTREGNFNKAAEIKYGLIPAL
                     EKKLATENTEDEAASKDKKTESLLREEVSEEDIAKVVSIWTGIPVTKMMESEMQKLLE
                     LETILHKRVVGQDEAVRAVSDTIRRNKAGLSDENKPLGSFLFLGPTGVGKTELAKTLS
                     DFLFNDEKALTRIDMSEYMEKFSVSRLIGAPPGYVGYDEGGQLTEAVRRRPYSVVLFD
                     EIEKAHPDVFNVLLQVLDDGRLTDSQGRIVDFKNTIIIMTSNIGSDLILQAQTNEDLH
                     KIANQIDAVLKTAFRPEFLNRIDETITFSRLDESAIASIVQLQLDRLSARLEAKRMKI
                     AFSKEALDFLSKVGYDPLFGARPVKRAIQSYVENP"
BASE COUNT         4008 a         2220 c         2577 g         3866 t
ORIGIN      
        1 caaaacgtac gtattcttga gcatgatgca ctcgaggttt tggaatatat gattcctgac
       61 aattctattt ctgcttttca tgtttttttt cccgatccct ggccaaagaa aaagcatcac
      121 aaaaggcgat ttatgcttcg accaaataca ggtcttttag caaaaaaatt aaaaaaagat
      181 gcgtgtattt atatggcaac tgattgggaa gattatgcct atcatgcttt agaagaattg
      241 accgcaactg aaggcttgaa aaataaatac gaggacctaa aaaacagctt gctaggtttt
      301 gcaccaaagc aagactggcg tccagaaaca ggctttgaaa aaaaagggaa aaatgcaaat
      361 aggcgtattt ttgatttagt ctttgtaaag tcttgaggcg aagataatga aaaaagagta
      421 ttcggtagaa gaattagttt ctcttataga aggctatcaa gcttcatact atggaggtga
      481 ggcggagatt tctgatgaag cctttgatca actttgggat ttattaaaag agaaggaccc
      541 agataatcca cttttacaaa agataggtag cgatattggt gaagctgaaa aaacaaaaac
      601 aggtggcgtt tttcaaaaag tacggcatct catccctatg ggaagtcaag agaaggctgc
      661 aaatccagaa gagtttcgag cctgggcaga taaaatgtct tttaaaaact ttctcgttga
      721 gtacaaactg gacggtgcaa gtttagaatt acagtttgaa aaaggcctgt ttacccgcgc
      781 ggtaactcgt ggcgatggtg aaataggcga tgatataacg aaaaatgttt taaaaatgca
      841 ggggcttgta aaatccttaa aagatgcttc tggaaatagg cttgatataa gtggcggcgt
      901 tcgcgctgaa gttattatga aaaaagaaat tcataaaaca tactaccctg ataaggcaaa
      961 ttgtcgaaac gctgcaaatg gcttgatgaa aagaaaagat ggctctggaa gcgagcactt
     1021 acatatactt tgttacgatg cttttttttc agggcaagaa gcgctgtata caaatgaaaa
     1081 agaaaaggtg ctttggattg aaaaacaagg ttttgaaatt gtgcctttta agctttgtca
     1141 gggtgcaaat gaagtaatag attttcgcgc acaggtaatg gaagagagag aaggcttaga
     1201 ttatgatatt gacgggcttg ttgttaaggg cttggaaata gaccttgctg atgcagaacg
     1261 tcggcgacca gaaaagcaaa ttgcttttaa gtttaccttg gaagaagcca taagcattgt
     1321 aaaaaatatt atttggagtg agtctggggt aacctatacc cccatagcgg aatttgaacc
     1381 ggtatttttg gctggtacga cggtacaaag agcaagcctt gtaaacccaa acactattcg
     1441 aaacctcggc attaaaatcg gcagccatgt tgtagttaca aaacgcggtg aaataattcc
     1501 caaaatcgaa tatgttttag aaggatataa gcactcgtat gaaaaaaacg agaatagctt
     1561 gggctctctc tttgatgaag aaaataatat tttccctaca aaatgtagcg cttgcaatac
     1621 aaaacttttt gatgagggaa caaggctttt ttgcccaaat aaagcttgtc cgaaacgtat
     1681 tcatcataga cttgaaaaat gggtagcaag tctggatatt cgcgaccttg gtatagggct
     1741 catagaaaaa ttatttttgt caggaaagct tagctctata aaagattttt atagtctcac
     1801 agaagatgat ctacgtccct tttttcttaa tgaagaaagt ttagaaaaag aaaaagaatc
     1861 cctcggcgca aagaaggttt accaatcaat tcaagacaga aaaacacttt cgctttcaac
     1921 atttatcgca ggttttgaca tagaaggttt tggcgaaaca caaattgaaa atctcataca
     1981 agcaggctat aaaaagctcg aagatttttt ttcagcctca gttgatgaga tagcatcagt
     2041 tcatggtttt ggagaaattt cagcacgcaa tttagttgaa ggtttagctg aacataagga
     2101 agagatgttg tttcttatat caaataaaat ccttgtttta cgtgaagaaa ctgaggcagc
     2161 cttaagtgga aaatcctttt gttttactgg tgagctgtat acgatgaaac ggagcgaggc
     2221 tgaagctttg gttaaaaaaa atggtggtgc ggttaaaagc tctgtcgtaa aagacctttc
     2281 ttttttagtc acaaacaata ttgaaagcgg ttcgagtaaa aataaaaaag caggtgagct
     2341 tggtataccg attatcgatg aaaaagaatt cttacgtttg attggccatg aatagaactt
     2401 tttttacgcg catccttttt tattttttta tactcatcgt ttttattttt tctgtgaata
     2461 ccgtgcatct tcttttaata ataaaaaata ttcaaaggct tcctgagcta ccgaagccat
     2521 atgaaatgat gcgttttgtt attaactcat acggagatag ctccgtcaat gcacgttttt
     2581 ggttttatga tctacaagga aatgagcttg cctctcttga gcgttcttgg cctgcagatt
     2641 atttgaaagt ccattttacg tatgcgcagt ataataaaaa aatgcttgtc tatcctacaa
     2701 caattcaaaa catacaagtc aataaaaaac gtcaagctca tggaacagat ttacgaccct
     2761 ttgtacaaat aaagacctat acaagagctc cgagaagtac agatgaaaat acagccagcg
     2821 aaaaagctct gagatctttg gaagcattta tactagataa gaatattttt aaaaaaactt
     2881 ttttctcgtt tcgtcctttt tttctagaag atcttagcct ttctttagaa agagctgaac
     2941 gtgggcgtat ctattccttg tactttgaaa atggacgagt ctatgtacaa tagctttatt
     3001 taaataaatc aaatcgttct acaaaataat ctggatttac cgcctcagcg agaagacgca
     3061 tttcccagtg caaatgtgga cctgttgcta agcctgtttt tccagaaagc cctatgagat
     3121 cacctttttg cacaaagtcg tcttttttta caagcatttt atctaagtga taatacaggc
     3181 tataaagacc ggggaaatgt tcaatgacta tcgtccaacc agtggagttt ctcatttcgc
     3241 tcattaaaac ttttccgctt gcacaggcaa atatttctgt tccgattgga actccaaaat
     3301 ctattccaaa atgcaagttg gtttctgatt tgccattgga ataggcaaag gttcttctgt
     3361 ctccaaaaaa acttgttcgt cgggtagagt gaactggttg cataaatgct tcaattgttt
     3421 tttttgcttc gagatttatt gtgttgagga ctgcatttaa gttgtctatc tgtgccattc
     3481 tttgcggcga atcatctgtt ttaatagcgg tgtttacagg atcgagcggg atagtctctt
     3541 ggacaaatat tttttcttta aggctaaacg ggataagaag cctttcgttg agtgcattgc
     3601 tttgaaaatc tatttgcatt gtgtagcttc ctggacttag ataggttgaa agtggtagac
     3661 cgacaaagag tatttttgta gttggattta tgtttccttc ttctagattg tacatctttg
     3721 cggagcgaat tctttttcct gttttatttt ctattatttc aacgcgagcg tttatttcat
     3781 tttttataaa agggctttcg acaaggagct ttagaaaaac gggatctccc ggatatagat
     3841 tttcgtcaaa aatacttgta attatatttg agccaaagcg aaatgttttt gaattggaaa
     3901 aaaaagggct gcttgtaaaa agagtaagca taaaaaagag aaaaagtttt ttttgtattg
     3961 tcatttttgt agatccatgt ttagagtttt tgtatatcgc taactatcat ttgtaaagat
     4021 tccataccgt taaaggtatt tctacttatt gtatatgcta gattgattct atcgcctgtg
     4081 tcaaaatctc gtttaagtcg ctttgattct cgccaaaaaa gagcaggcca ttttgtttta
     4141 tcaacagcga gagttagttt taagtgaaag ggttcaacct ttccaagtat atccgcattg
     4201 agaattttta atttatctga ggcaaaaagg aggggagggt tttcttctcc atagggttca
     4261 aataaatcaa ttgtttttag aatttcttgt gtcaaatgtt tttcctgtaa ttgcgcatcg
     4321 atagcaagac tttcttccac atcagaaatt tctaagttcc ttgaaattgt ctttaagcga
     4381 ttaagtagct caggaagttt ttctttttcc aagccaaaac ctgcggcaaa ggcatgtcct
     4441 ccaaaatttg taaataaatc gctacattgg tttaggatag gcataacttc aaggttcttg
     4501 tttgaacgga ctgagcctat tatgctatct tcaaggcttg taagtataaa agcaggcctg
     4561 ttactaagtt gtgaaactcg agcggctatg atgccggtaa cgccacgatg aatacgctcg
     4621 tcatagataa caacaatgtt ttggtggtag tcgtcaaggc ttttaaatat cgcattttct
     4681 gcatagcccc aagcctcatt tccgagctct tttcttttat tgtttatttc tataattgat
     4741 tcgcagattt catttcgttt tttttgctct gtttctagga gcagctcaag ggatatttct
     4801 ggactaccaa gccttcctgt tgcgtttagg gcaggcgtta tggaccaggc gatatcggtg
     4861 cttgttattt tttttccgag aagattttgc cgtgccataa gctcttgcaa acctggtcgt
     4921 attcttcctt catttatagt tttgagggct tgttttacaa agaggcggtt ttcatttttt
     4981 agcggcataa tatctgcaat cgtggatatt gcaagaagtt gtaaatcgct ttcatccttg
     5041 ttgagtccta agtcattatt attttttgtt ttggagtcac cgagtttttg cataaaggtg
     5101 ataaagatat taaaaaaaac atctatctcg gtattttttt caggatgata gcgcgcaatt
     5161 tttgaaaatt gtttaagccg taaaagactt aaatccttta ccgagggtat gaggcttgcg
     5221 atatcgtttt gaatgtcgta gagattaaat tcaattcctg ctccaaaagc atcctttaga
     5281 agtttttttt ctattgcttc atcccagaca aagatttgtt gcccttgcaa gaattctggg
     5341 agccttgttt ttgatatgct gataagccct ggaacaatgg tttcactcaa gcgctctttt
     5401 tcacacatgt tttgtatttt aatacattct atttgatagg catctttcag aggacgtacg
     5461 tgtagcaagc aaatctcttg tttataggca tcgctttggg caaaacgcaa ggctgtagca
     5521 agcttatagg caactgcaca gccagaaata tgctcaaaag gatagtcctc tccttccatt
     5581 tttgggttga taataataca atcttcaggc aatttttcag gtggattgtg atggtcagta
     5641 atgatgacag aaatgccttt ttcatgggcg tattggattt catcgtagtt cgatatacca
     5701 cagtcgacgg tgattataag agagccatag ttttcagcaa aatcatcgat tgcttgtatg
     5761 tttaatccat atggctcatc atctttggga agcctgtagc tgacatcaat tccgaggtct
     5821 tttaagcatt catagagcaa aaccgtactt gtaataccgt ctacatccct atctccaaaa
     5881 ataagaactt tttcaccttc agctttggca tccataatgc gatcgacagc atcttccatt
     5941 tcattaaata aaaagggatt gtgttgatag cgtaaatcat cttctaaaaa atacaagatg
     6001 tcttcgccct ctgttatgcc ccttcgtgcc aaaatcgttg aaacaagggg accacaagaa
     6061 tagcgcgtat caagggcctt aataatttct tttgagattg cttctttttt ccaaattgcc
     6121 atgagattcc ttaacctagt aatatacctt ttactctacc atgaatagga gtcaaaatct
     6181 agcactaaaa gattttctat gctatagtca tttttatgaa tgaaaaaatg ctgcatataa
     6241 atgatgtgta caatggtttt actgttttag atataagtcc tattcttgac tataagtctg
     6301 agggtatttg gcttcgccat aatgaaactg gcttagaaat atttcatatg tataatgatg
     6361 acagtgaaaa tttgttttct tttgccttta aaacaattcc aagtgattcc actggtcttg
     6421 cccatattat ggaacattcg gtactctgtg gctcaaaaaa ctacccctta aaagatccct
     6481 ttatacgcat ggcaaatcag tcggtaaaaa cctttttaaa cgccatgacc tttcccgata
     6541 agacagtcta tcccgccagt tcaaccgtag aggcagatta ttttaatctc atggcggtat
     6601 atgcagatgc agtttttttc ccattattaa aaaaagaaat ctttatgcaa gaagcacatc
     6661 gacttagtct cggagaggat ggggcagttt cgattcaggg tgttgttttt aatgaaatga
     6721 tgggaaacta ttcaagtttt gacagcatgg ttcaggaaaa aatcgtacaa acaattttgc
     6781 caggtacaac ctatgccttt gattcaggag gggatccaag agagattccg cttttaagct
     6841 acgagcgatt tttagatttc cacaaaacct tttatcagcc ttcaaactgc cgcctttttt
     6901 tatacggcaa tatacccaca acaaagcaaa tagacttctt aaatgaaaaa gtgctttctc
     6961 agtttaaaac agcaacgaat ccctttagcc aaatgttttt atcaaagcag gatagcgatt
     7021 tttgcgcaca aacacaggtg agggcattca cagcaccgca aaaccatgtt tgttatggtc
     7081 cgagtgtaaa aggtgctggt tcagaaaatg gggatagtct tttactcacc tggcttttgg
     7141 gctttaccag tgatccgcag agctacatgg aatccctttt tttggcagaa ttactccttg
     7201 gccatgacgg cgctcccctt gcgaaggcct tgatagaatc aggtttaggg gaagatttag
     7261 agcctgcaag cggcatagaa acagacctac gctatatctt gtttactgcg ggtatgcgnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac ctttgctgcc attaaaaaaa
     7441 gaatttatga agatgatagc tatcttgcaa aacttttaga aaagtacttt ataagaaatc
     7501 agcatagagt tcggctcatg atgaaaagta gccctgactt tgaaaaagaa ctacaagaaa
     7561 aagttgctct agagacaaaa cggctgtttg aaaactatgt agagctgtat gtagaaaaaa
     7621 aaggaatttc taaaaccgaa acacaatctg taaaagaaat tgatgagaaa atacgttcga
     7681 ttattcaaaa agaaaatgaa gatctcgcat cgtttcagga agcagaagaa gatggtagct
     7741 tgctttcttt actgccccac ttaaaaccaa aggatttgga ccccagtatc gatataatcg
     7801 aaacaaagcg gagagaaata cagaacattc cgacattttt acatgagcaa gaagtaaatg
     7861 gtatcttata ttttaccctg ggttttcctt gcgaccttct tgaggcaaaa gactatcttc
     7921 tacttccttt tttttgcatg gcagcaagca attcaggatt tgattccctt gactgggctg
     7981 aggcatcatc gaaacttgca caggcgacgg gaggcttttc tgcctcgccc ctctcttctc
     8041 ccattgatga ggagcttaaa aggtccttgg gtatagtcga tgagaatggt aaaacaatcc
     8101 tccttgagga agatgcaaca gaacgtcttt ataaggctga tcccagtctt ggacgctctt
     8161 ggattttttt caaaatgaaa acacttgaag aaaaatccct caaagcctta gaattagttt
     8221 ttagctgtat taaaaatgta gatttttctg ataaaaaacg tatagaagac ttactcatcg
     8281 agtacaaaaa tggtttagtt tcctcgatta ttccaggtgg aactgcctat gcggtttctc
     8341 gtgcgaattg cttttttagt cgtgaaacag ctatcgatga gatttggtca ggcttaacgc
     8401 aaattgcttt tataaaagag ctgagtaaaa agaacattga tgaagtgatt cagtctcttt
     8461 tgcgtataaa aaaacagctt ttagaaagtg gtgcaattat caatataatt gcagaaaagc
     8521 aatctatgcc acgtttgatt tctgaagttg aaaaaggaat gcaagacttt ttcccagagg
     8581 ttcaggcacc gaaaaaaccg cgtttacaaa aagatgaggc tttttttgaa cttcttcgtt
     8641 tttcccccaa taccgatgat aaaacagaag tgtttatttt accgacacag gtcggttttg
     8701 cagcgagctg tttttctatg cgagggaatg aaaaagagca agttgcaatc tctgttttag
     8761 cacattatct tacaaacacc gctttttggg cacaaatacg caccatggga ggagcctatg
     8821 gggcacaagc tttttacaat agcttagaag cagtttttct ctgtgcaacg tttcgggatc
     8881 caaacccaga acgaagcctc gctgtttttg ttgatgaatg taaaaaactt tcatcgcttt
     8941 cctttgatac aaatcttata gaaaagctca taactggttc gtatagcaaa gaaatacagc
     9001 ctaaaacccc ttcgataaag ggaagtttag gttttattcg tagtttatat ggaataaatg
     9061 atgaaaaacg tcaaagaagg atgcagtatc ttttagaaat ggatgaagaa accttaaaaa
     9121 aagtttccaa ggatatagct catagtcttt ttgatatgta ttccgcagta atcaatcaaa
     9181 aagcactttc aaagactgga aaaaatattt attttgacgt ataataccat gggctgagaa
     9241 ttctaaaaaa tcaggtagct atctaccata attataatga ggataaaaaa tgaataaaaa
     9301 actagaagaa tgtgtaaaga agttacgaaa tctcgtttca gatgtacaag gtgcaccata
     9361 tcctgggaat gtaaacgaag aactatatta tatttggtat gaacatgcgc aaaaaaatgc
     9421 tatggaatgc tttgaattct tagatgaata ctttccaagg gaaaaagagg aaataaaaaa
     9481 agatttatta aaagtgatgt aaaaacaaga tgataggagc tatttcttgt tttatttctt
     9541 ttgttctcat cgtacaaggg gtttaatgat gcaaactaga tgcggtttta tccataaacc
     9601 tttcgattta aaaagtctcg ggaaatcaaa gcaatttgaa atactgcgtt attttattcc
     9661 tgtaaagcat aataaaacag gacacctaga tgtttttttt attgaacttt tttctattaa
     9721 cccttctttg tatactcggc aactgcaatt tgcttctatt gcagaaaaaa aaataaaggc
     9781 agagagatct tcttttattt tagttcgggc agggctcatt gcccatgggg aaaaatgtaa
     9841 gcatattttt tttacgagta gagatatacg gtatgataaa aaaaataaaa gaatgcaaat
     9901 tggctcttct ttttttgctt cagatgctat ttctggttcc attgagcaag aatcgcagcc
     9961 tatagaatgg aagataactt ttacttcaaa agaattcctg tcttattttt tatctcatcg
    10021 tttttatttt tcaaaaaaag atagattaaa aacnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnggctata gttttgagag cattcaaaga ttagatacga atcatctttt
    10201 ttctcttggt gcaaagggct acggtgaact gagattgtat aaaaaaaaga aaaaaaatct
    10261 tgagataatt gaacatgtgc gcattgaaaa ttgtctttgt gagcaagcct taaatttccc
    10321 cccgaatata taaaatgctt gcattttgcc ttggcttttt gtacatttaa actatgaatt
    10381 atgatagatt aactataaaa gcacaggaag caattcaagt tgcatcgact atagcacaac
    10441 aagaggacca ctctgaaata ggcagtgagc atcttttgtt tgcgcttctc gaccaaaaag
    10501 atggtattat tccaccactt gttgaacgag tgggggtaca ggtatctacc ttactgaatg
    10561 atgtcgaaga acttctcgat gtctatcctc gtgtaacagg aaatgtacaa gtcgctttat
    10621 caaacgatgt tcaaaaaatc ttagcaaagg ctgaaaaaga aactgaaaac ttaaaagatg
    10681 actatgtttc aactgagcat atctttttag ctctagaatc ctctcattca aaagtaacac
    10741 aagttttaca ccgttatggt attactcgtc atgctatctt acaagcgctt agttctatac
    10801 gtggaaacca gagaattaca agtcaagagc cagaagcaac gatgcaatct ttagaaaaat
    10861 actgcaagga cctaacacag cttgctcgtc aagaaaaaat agaccctgtt attgggcgag
    10921 atgaagaaat ccgtcgcgtt atgcaagttt tatctcgaag gacaaaaaac aaccccgttc
    10981 tcattggaga gcctggagtt ggaaaaaccg ctattgctga gggcttagcg cgccgcatgg
    11041 tttccggtga tgtccccgat agcctcaaaa acaagcgctt gcttgccctc gaccttggag
    11101 cccttgtcgc aggtgctaaa tttcgcggtg aatttgaaga gcggctcaaa gcggttatta
    11161 atgaggtgca aaaatcagat gggcagatta ttttatttat agatgagctg cacacactcg
    11221 ttggtgcggg ggcatcggaa ggttccatgg acgcatcaaa tcttcttaag ccagctcttg
    11281 cgcgaggtga gcttcgggca atcggggcaa caactcttga tgagtatcgt aaatatattg
    11341 aaaaagatgc tgcccttgaa agacgttttc aacaagtcta ttgtgccgag ccaagcgtag
    11401 aagataccat cgccatactt cgaggcttaa aagaaaaata cgaagtgcat cacggggtgc
    11461 gtataaaaga tgaagccttg gttgcagcaa gtttgctttc caatcgctat attaccaatc
    11521 gatttttgcc cgataaggct atcgacttag tcgatgaggc agcgagtaag ctcaagatgg
    11581 aaattgaaag tcagccaact gaactagacc aagttgagcg acgcatcatg caattgacga
    11641 ttgaaaaagt atctcttgca aaggaagagg atgcctcttc taaggagcgc ctcgcaaagc
    11701 ttgaaaaaga acttgcagac atttctgaaa aacgagatgc aatgaaagca cagtgggaaa
    11761 atgaaaaaaa acgcatcgat gaaagacgac atgtaaaaga agaacttgag cagctgcgca
    11821 atgatgaagt gcgttttact cgtgagggca attttaacaa ggctgcagag ataaaatacg
    11881 gtttaattcc cgcgcttgaa aaaaaacttg ccacagaaaa caccgaagac gaggctgcat
    11941 cgaaggataa aaaaacagaa agtctcttac gagaagaggt gagcgaagaa gatattgcaa
    12001 aggttgtttc catttggacg ggtataccgg taactaagat gatggaaagc gaaatgcaaa
    12061 aacttctcga gcttgagact attttacaca aacgggttgt cggtcaagat gaggcagttc
    12121 gtgcggtaag cgataccata cgtcgaaaca aagcgggact ttctgatgaa aataaaccct
    12181 tgggtagttt tttattttta ggtccgacgg gagttggaaa aaccgagctt gcaaaaacct
    12241 taagtgattt tttatttaac gatgaaaaag cgcttacacg tatcgatatg tctgagtata
    12301 tggaaaagtt ttctgtaagc cgattgatag gtgcacctcc tggctatgtt ggctatgatg
    12361 agggtgggca gttaaccgaa gcggttcgcc gtcgccccta tagcgtagtg ctctttgatg
    12421 aaattgaaaa agcacatccc gatgtattca atgtactctt acaagttttg gatgatgggc
    12481 gtttaacaga ttctcaaggc cgcattgtcg attttaaaaa tacgattatt ataatgacga
    12541 gcaatattgg cagtgattta atcttacaag cacaaacaaa tgaagactta cataaaatag
    12601 caaaccaaat tgatgcggtc ttaaaaacag cctttagacc agagtttcta aaccgtatag
    12661 acgaaacaat cacctttagc cgccttgatg aatccgcaat tgcttcgatt gtccagttgc
    12721 aacttgacag gctgtctgca aggctcgaag caaaacgcat gaaaattgct ttttcaaaag
    12781 aggccttaga ctttttatca aaggtaggct atgacccctt atttggtgcg cgaccggtaa
    12841 agcgagcaat tcaaagctat gtagaaaatc c
//
LOCUS       DAOSSW010000050        12760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_4937_length_12760_cov_6.724124, whole genome shotgun sequence.
ACCESSION   DAOSSW010000050 DAOSSW010000000
VERSION     DAOSSW010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 12760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12760
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4937_length_12760_cov_6.724124"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="PKW26_05440"
     CDS             complement(<1..1173)
                     /locus_tag="PKW26_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93043.1"
                     /translation="MKIKRIFIVLFLFQNLCFAQTQNWVLAASPFEIQTDLDTIPEYT
                     KKRVEELQVLLPKMLLESFDKDLTREVSVPELIAKERYKLEVKKRALYKDYDTSIKKR
                     DAFFLEAKSKYLINQSIKSEEKKILDIETKIKNIQKEMIKLSEENTAFIPLHKKIVSY
                     NNAQALFSPSKKTLGEKKEERKSSEEKIALELEALEKNIDALISADIEVNGEYLELSL
                     VLDYFPSSLGSFSFVISSPLSDISYAISQMYEKLLPEILNEKLIGLQFAIEPAEVLDT
                     LHLHVDDEVLKKNESRVFIPKGEHSIFAEAEGYEPISFVYNFDSKSDFELRLDFKKIE
                     AKEIGLEAKDSEVLISLNAENQGALPKQVNLNKQTYLGHITDDEWSSFFLLQEKDFT"
     gene            complement(1170..2292)
                     /gene="prfB"
                     /locus_tag="PKW26_05445"
     CDS             complement(join(1170..2222,2224..2292))
                     /gene="prfB"
                     /locus_tag="PKW26_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704758.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOQ93044.1"
                     /translation="MIEEYKPKMESLKKDVLEIWGRLDVPSIVKKQKEKEALYEDPHF
                     WDDSEKAEKIVLEIKNLKSRYEPWQKLRADLDDLEVLYELTLEAADAEAFKDFEKNFE
                     KLNAEYKRLHILNLLDGEVDKNSAFLTIHSGAGGTESCDWASMLSRMYTRWAELKGYK
                     LTVLDLLEAEGGIKSITMQIEGDYVYGYLNTEAGIHRLVRISPFDSNARRHTSFSSVF
                     VFPVLDDTIDVDIKAEDLRVDTYRAGGAGGQHVNKTDSAVRFTHIPTGIVVACQSERS
                     QIMNRAQAMNMLKARLYDYYKEEKEKENEKFAGEKKSVSWGNQMRSYVFQPYTMVKDH
                     RTKYETGNIQAVMDGHIDQFIESFLATKWKGLPMGDDED"
     gene            complement(2340..2621)
                     /locus_tag="PKW26_05450"
     CDS             complement(2340..2621)
                     /locus_tag="PKW26_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas2"
                     /protein_id="HOQ93045.1"
                     /translation="MFVSVVLDPGQLDSARSLSSILAQYGFEKKQRACWEHNGMNEKK
                     LGLLKKDIDRVTDYYDSVRLYQYPIDGMLVITELQKKKWKKCMLRSTER"
     assembly_gap    2744..2843
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2844..3467
                     /locus_tag="PKW26_05455"
     CDS             <2844..3467
                     /locus_tag="PKW26_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93046.1"
                     /translation="SKDISQNKVAMYYYLISIDTQAEIIPFIIPGTNALAYIQMGNTK
                     EHPNGINNTAHTKQILQNTDQRSFFPLPKKNNEAEKNLDEVFFAESKTFENLKKQNNK
                     KRLITKTQVFHDDLEAKAGEAYLLAEILQNSFINLQFKESEEAIRSFLMIHRSQDIRY
                     KALFYLAQALYFQGDYIEALFYFIECQNYLFDVSRDWIDACLHILSE"
     gene            complement(3457..5427)
                     /locus_tag="PKW26_05460"
     CDS             complement(3457..5427)
                     /locus_tag="PKW26_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ93047.1"
                     /translation="MKKRFLIFCFFSLSFLFAQTALDFYTRGQQYQENAAWMKAIEAY
                     QEALNVNPSYAAAWFALSESTYALGEYALALQYAESAERFIRNDRALLNLKGFIYIGL
                     GEIKKASDLFLQVVKSYPNDIDARFGLAELELFSGKKTAAERYYKETLARQGENKKAL
                     LSLAILSYDMGNIRASKDFIQTALRYHSGESEVHYFASYLAVQDGDLALAEKHAKNAL
                     HLQPKNAKTIRLLSLIHYSQKKYNEATENLDRLLSLDKLDSFSWYLKAKIAEKQHNTE
                     GALVFYQRCLEINPEDEIARAAMEICITKATPIEDARRKGWAAYQLKKAQEATRLYLS
                     ETARYHYKRALKINPLDTDTRLLYAGLLLQDGRAESYLSQLEFIQNEGAKSLALSDLI
                     ESYASLLRSSLSQKWKTNPLYLDKTRYRLSLFYLDEGVHILHPDSLAIASQMIKETAE
                     MQGLMQVSTEDSSVASYAEAFQKAREKGTDLFAILSFEETEREVLGELTLYNAKTGTK
                     IQTISVYRTGNDRFTQTIQKLVSQLTKALPLRGRIIARSGYTALIDLGKTEGIEKNME
                     LSIVEKGRLSLDSASLRLLTQENNILGSISIEEVGEEMAEGRVSYTGFFDKIGVGDEV
                     FIALPLEDTEGKNLSSNQNTTPVLIDLLRSIR"
     gene            complement(5424..6227)
                     /locus_tag="PKW26_05465"
     CDS             complement(5424..6227)
                     /locus_tag="PKW26_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOQ93048.1"
                     /translation="MRFIKKLLSKKAEKEPGSQSRPETDTITRELLNEEKRYMIRGIE
                     DLSQTSVKEAMIPRIDVDFVPLNSSEEELLEIVAESGHSRFPVYSDSIDNVEGVLYVK
                     DLIKVLLKKEPIDLNQIIRKAFFVPESKRLDTLLREFKRRHVHIAIAIDEYGGVSGII
                     CMEDVIEEIVGDIQDEFDNEGEDILSLGEGIWLCDARVNIEDLNEAIGSDFSNEEFDT
                     LGGYVFDLFGKIPVKYEKVSSSEYDFIVQDMEGHKINLIKLIKKDKDLE"
     gene            complement(6224..6712)
                     /gene="ybeY"
                     /locus_tag="PKW26_05470"
     CDS             complement(6224..6712)
                     /gene="ybeY"
                     /locus_tag="PKW26_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOQ93049.1"
                     /translation="MKKRIIVDVEESFPKPVWLDKISPFVSAVLDDLALEDWELSILF
                     CSNAFIAKLNKEYRGKEGPTDILSFEQGDEYVDEDGESWFIAGDIIISLEMLQSNAVS
                     YDISEDEELKRLLIHGILHLDGYDHGEDHIQAGKEAQNAMLVLQEKLLSTYTIKIIED
                     IA"
     gene            complement(6709..8499)
                     /locus_tag="PKW26_05475"
     CDS             complement(6709..8499)
                     /locus_tag="PKW26_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOQ93050.1"
                     /translation="MNKKTIHNNITLEENSSFSHFWKTFRQHAKQNTTSFMIIALSFL
                     AVLAVAFFDVASKETIVSFQLQEFELGQISDRTIISSKNIAPTVEHPITVEKNEKIIK
                     KGFPITEEGYEKLQKLAASPTYIDYRAFANALIFYVLLAALAIFLFSSKICGKKMDTK
                     EQVFLAVLFVFVQLITVIASKMTFFDTSFALPILIPASLSVMLLTVLFGQQNAIFFVF
                     LLAFSVLFITGFDPIPAFFLVASSITAIRIVRRIEKRIDMIFASFLLGLFNAIFLLCI
                     KIIFNNPQTFSLSIILGNVFNGFVSGILALGLLTPLESLLNTASVFRLMDLSDLNNPV
                     MKKMLIVAPGTYNHSMMVASLAESACHEIGANALLARVGAYYHDLGKTEQPEYFVENQ
                     TGNNKHEELNPRLSVSVIRRHVKKGLEKATQLRLPKEVLAIIEEHHGNSLISYFYAEA
                     KKQDPQVSAEEFSYIGTPPQSKESAVVMLADTVEAACRTLDKPSVSRLEKFIKQLVMT
                     KVELGQLDNSGLTFKELSSIQRAFVTILAGYYHSRIEYPNQKDPDEEETGEVKNEKGT
                     KDSDKKNTKHKTQNANSVLIKKSKKDVKEK"
     gene            complement(8537..11788)
                     /locus_tag="PKW26_05480"
     CDS             complement(8537..11788)
                     /locus_tag="PKW26_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93051.1"
                     /translation="MKKSLFFIVCTLGFIFFAFPQEDAKQKTSTVTIEKAEKTEYLKD
                     EKTGEETIVFKGNVLLSVESGTDKTKISASLVRFNRKQNMLYAEGAVVLEKTTASQPA
                     ERIDADAILFNIDSLEGVFDDVRVAQEKLSALQLPEGSKLIVYADLLAKDKASTIAFD
                     KGKLTFCSDEEEPHWHLKASKIWLLPGNEFAFFNALLYVGNVPLFYFPFFYYPKDELI
                     FNPVFGYSEREGYFLQTTTYFVGRKNLEVKKQADKTDKTDKTQSTETIEELFNFLKPT
                     TLKEQERRGLFLHNLETDAKDVSSDYLKFMADYYSDLGAYLGIKGSFSPNQLVKKIDF
                     GFGLGFSRSFFPNSPVPTINPATGERVWDQSFLFGTVLPFRFYEEFSLSLSKPSFSFS
                     LSLPFYSDPYFNADFHNRSEDMDWINYFLNNTLMNTPNLQKNKVALSSFNWNLNLSYS
                     PKIDFIKPYINTLSLSNLTSSVDFITKQNAEKTDSKFIHDINRQFYIPSQIKPVDANM
                     KLSGTLFSYPFAQKIKNTKQDIEDIFLFAPKAEDFSEEKKQEADKNEIEIENKESLSE
                     DYKNQEIMNQVVSEDLDEVAKELTAVKLNPTKLTKSAFTLFSYSLSYDLSSSLKTLVL
                     LNSSEINTPEDMTASFSNLQSTYFQLRSPLNLRSKATFINAIFQMNNTFSFIPQIQDH
                     PFISEEYYTNQVDIDKIKLNDLAENKFDITNKNSLSVNPFLSHAVFKNTSLTWNLDIR
                     LLKKEFTGTLEEPLWSYIYPEWDKESIRVHNLAFLLSLDEGIFAQKLSFSLSLPPTTE
                     SYGTKLSLVFPLLTTSFETSIKKDLKTEEYRFDPFFQTAQLKVFNNSVILDQNYKYDI
                     EEKRSTSFYTGLSYASFSMRYSMLYTNLYKLTENGWLSTDEKDFIPFDLRFSFSKTNL
                     SWSFWKNRISINPGLSTSFTMDLVRPTNSYFNFSPSLSFSIYNFLKITFSSESTNNLV
                     FRYFQNFYGYDFKVPGETNIFIDLLKSFDFMNEKNRMESAFKLQKLSLKIEHDLHDWT
                     LKSEFSFSPRLITKTSPYYYDFSPYFSLSVVWNPLKSLKTEIVDEYGTFQLNP"
     gene            complement(11788..>12760)
                     /locus_tag="PKW26_05485"
     CDS             complement(11788..>12760)
                     /locus_tag="PKW26_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOQ93052.1"
                     /translation="NISVSIPLGTFTCVTGVSGSGKSTLISDVFYPAVSNALMRSTKS
                     VGAHKKISGLEHIDKVINIDQGPIGRTPRSNPATYVGVFNGIRDLYASLPEAKMRGYK
                     PGRFSFNVKGGRCENCQGAGTITIEMNFLSDVYINCEVCQGKRFNQETLEVRYKGKNI
                     YDVLNMTIEEATEFFIHIPHIARKMDTLMSVGLGYIKLGQSALTLSGGEAQRVKLANE
                     LSKRSTGKTLYILDEPTTGLHFADVKQLMEVIQRLVDTGNTVVMIEHNLDVILQADHI
                     IDLGPEGGFRGGEVITVGTPEAVAACEKSHTGFFIKEHLKALNRIGK"
BASE COUNT         3878 a         2429 c         2234 g         4119 t
ORIGIN      
        1 ggtaaaatct ttttcttgca agagaaaaaa actgctccac tcatcatctg taatgtgacc
       61 aagataggtt tgtttattta gatttacttg ctttggtaaa gcaccttggt tttctgcatt
      121 taatgaaatc aaaacctcag aatcttttgc ctcgagacct atttcctttg cctctatttt
      181 tttaaaatct aatctaagtt caaaatcaga cttgctatca aaattgtaaa caaaggatat
      241 cggttcatag ccctctgcct cggcaaaaat agaatgctct ccttttggaa taaaaactct
      301 gctttcattc tttttcaaaa cttcatcatc gacgtgaaga tggagcgtat cgagcacttc
      361 agcaggttca atcgcaaatt gtagccctat tagtttttca tttaaaattt ctggcaaaag
      421 cttttcatac atttgtgaaa tagcataaga aatgtcagaa agcggtgatg atataacgaa
      481 ggaaaaagag ccgaggctgc ttggaaaata atctaaaaca agggataatt ctagatactc
      541 accatttact tcgatatctg cacttataag cgcgtcaata tttttttcca aagcttcaag
      601 ttctaaagca attttttctt cactggattt tcgctcttct tttttttctc ctagagtttt
      661 tttactagga ctaaacaagg cttgcgcatt attataggaa acaatttttt tatgaagtgg
      721 tataaaggct gtgttttctt cagaaagctt tatcatttct ttttgtatat tttttatttt
      781 agtttcaata tcgaggattt ttttttcttc gctttttata ctttggttga taaggtattt
      841 tgatttagct tcaagaaaga atgcatctcg tttttttata cttgtatcat agtccttgta
      901 caaagctctt ttttttacct ctagcttata tcgctccttt gctatcagct caggaacgct
      961 tacttctcga gtaagatctt tgtcaaagct ttcgagaagc attttgggta agagcacttg
     1021 caattcttca acacgttttt tagtatattc aggaattgta tccaaatcgg tttgaatctc
     1081 aaaaggactt gcggcgagaa cccagttttg cgtttgtgca aaacaaaggt tttgaaaaag
     1141 aaataaaacg ataaaaattc tttttatttt caatcctcat catctcccat tggaagccct
     1201 ttccatttag ttgccaaaaa agactcgata aactggtcga tatgtccatc cataactgct
     1261 tgaatatttc ccgtttcata ttttgttcga tgatctttta ccatcgtata gggttgaaac
     1321 acataagagc gcatttggtt tccccatgat acggattttt tttctcccgc aaacttttcg
     1381 ttttcttttt ctttttcttc tttataataa tcatataatc gagccttcag catgttcatt
     1441 gcttgcgctc gattcataat ttgacttcgc tcagattgac aggctacgac gataccagtc
     1501 ggtatatgtg taaaacgtac cgcagaatct gttttattta catgctgacc accagcacca
     1561 ccagcgcgat aggtgtcaac acgcaaatct tcagctttga tatctacatc gattgtatca
     1621 tcgagtacgg ggaagacaaa gaccgaagaa aaagaggtgt gacgacgcgc atttgaatca
     1681 aaggggctaa tgcgaactaa acgatgaatt cccgcttcgg tatttaagta gccatagaca
     1741 taatcgcctt ctatttgcat agtaatagat tttataccgc cctctgcctc taaaaggtcc
     1801 aaaacagtca gcttataacc ttttagctct gcccacctcg tatacatacg actgagcata
     1861 gaagcccaat cgcaggattc agttccacct gcaccagagt gaatcgttaa aaaagctgag
     1921 tttttgtcaa cttccccgtc aagtagattt aagatatgga ggcgcttgta ttcagcattg
     1981 agcttttcaa agtttttttc aaagtcttta aacgcttcag catctgcagc ttcaagcgtc
     2041 agttcgtata atacttctaa atcatcaaga tcggcacgca atttttgcca gggttcataa
     2101 cgcgatttta agttttttat ctctaagaca attttttctg ccttttcact atcatcccaa
     2161 aaatgcggat cttcatacaa agcttctttt tctttttgtt tttttactat gctcggaacg
     2221 tcaaagacgc ccccagattt ctaaaacatc tttttttaaa ctttccattt ttggcttata
     2281 ttcttctatc attttgtcct cttttctgta tttccgaaat tacgaaatct ttatgcaagt
     2341 cagcgttctg ttgaacgaag catacatttt ttccacttct ttttttgcaa ctcagttata
     2401 actaacattc cgtcaattgg gtattggtag agacggacag aatcgtaata gtcagttaca
     2461 cgatcgatat cttttttcaa aagaccaagc tttttttcgt tcataccatt atgttcccaa
     2521 catgcccttt gttttttttc aaagccatat tgtgcaagaa ttgaggaaag cgagcgagca
     2581 gaatctaact gccctggatc caaaacaact gatacaaaca tatctataga ataaagtaaa
     2641 gtcgctctct tgtcaaatcc ttcattatgt atcacaatgg aaatatgaaa aaaatgcatt
     2701 taaaacaaat tctttgcttt ttattcagct ttctcataag tttnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnagcaagg atatatcaca aaacaaagta gctatgtatt
     2881 attatctcat atcgatagat acgcaagctg aaattatacc ctttataatt ccaggtacaa
     2941 atgccctcgc ctacatacag atgggaaaca cgaaagaaca tcccaacggc ataaataata
     3001 cagcacacac aaaacaaatc ttgcaaaata ctgaccaaag atcttttttc ccccttccca
     3061 aaaaaaacaa tgaagcagag aagaatctcg acgaagtttt ttttgcggag tcaaaaacct
     3121 ttgaaaatct aaaaaagcaa aacaataaaa aacgacttat aacaaaaaca caggtttttc
     3181 atgacgacct tgaggcaaag gcaggagaag cctatctttt ggcagaaatt ttacaaaaca
     3241 gctttataaa cctgcagttc aaagaaagcg aagaagctat ccgctctttt ttgatgatac
     3301 atcgcagcca agatattcgc tacaaggcgc ttttttatct tgcacaagcc ttgtattttc
     3361 agggagacta cattgaagcc ctattttatt ttatagaatg tcaaaattat ctttttgatg
     3421 tcagccgaga ttggatagat gcctgtttac acatacttag cgaatagagc gtaacaagtc
     3481 aatgagcact ggtgtggtgt tttggtttga tgagagattt ttcccttcag tatcttcaag
     3541 aggaagggca ataaaaacct catcgccgac gcctatttta tcaaaaaaac cagtgtagct
     3601 cacccttccc tctgccattt cttcaccaac ttcttctatc gatatagaac caagtatatt
     3661 gttttcttgt gtaagcaaac gtaaggaagc agaatccaag ctcagccttc ctttttctac
     3721 gatgcttagt tccatgtttt tttctatacc ctctgttttt cccaaatcaa tgagggcggt
     3781 atagccagaa cgtgctataa tgcgtcctcg caaaggaagc gcctttgtca gctgggatac
     3841 gagcttttgt attgtttgcg taaagcgatc atttccggtt ctatagacag aaattgtttg
     3901 tatttttgtt cccgtcttcg cattatagag agttaattct ccgagcactt ctctttctgt
     3961 ttcttcaaag ctcaaaatgg caaaaagatc cgttcctttt tctcgtgcct tttgaaaagc
     4021 ttctgcataa ctcgcaacag agctatcttc tgtagaaacc tgcataagtc cctgcatttc
     4081 ggccgtttct tttatcattt ggctcgctat agctaatgaa tctggatgca aaatatgaac
     4141 accttcatca aggtaaaata aagaaagcct ataccgtgtt ttatcgagat aaagcggatt
     4201 tgtcttccac ttctgcgaca aagaagaacg caataaagaa gcataggatt ctattaaatc
     4261 tgaaagagca aggctttttg caccttcatt ttgtataaac tccagctgtg atagatagga
     4321 ttcagccctt ccatcttgca ataaaagtcc cgcatacaaa agtctcgtgt cagtatcgag
     4381 ggggtttatt tttagcgccc ttttatagtg ataccttgct gtttcactca agtataagcg
     4441 cgttgcctcc tgggcttttt taagctgata tgcagcccag ccttttcgac gagcatcttc
     4501 tatgggagtt gcctttgtaa tgcatatttc catagcagca cgggcgattt catcctcagg
     4561 attgatctct aaacaacgct gataaaagac caaagcgcct tctgtattat gctgtttttc
     4621 agcaattttc gccttcaaat accatgaaaa agaatccagt ttatcaagag aaagcagacg
     4681 atcgaggttt tctgtcgcct cattgtattt tttttggcta taatgtatga ggctcagtaa
     4741 acgaattgtt tttgcgtttt tgggctgtaa atgaagtgcg ttttttgcgt gtttttctgc
     4801 aagagcaagg tcgccatctt gtacagccag atacgacgca aaataatgta cttctgactc
     4861 accgctatga tagcgcaaag cggtttgaat aaaatctttt gaagctcgta tatttcccat
     4921 atcgtaggat aaaatagcaa gagaaaggag ggcttttttg ttttcgccct gtcgcgcaag
     4981 cgtttcttta tagtaccttt ctgcagcagt tttttttcca gaaaaaagtt ccagctcagc
     5041 taaaccaaag cgtgcatcta tatcatttgg atagcttttc actacctgca aaaataaatc
     5101 acttgctttt tttatttccc ccaagccaat ataaataaaa ccttttagat tgagtaaggc
     5161 gcgatcattt cgtataaaac gttctgcgct ttctgcatat tgtaaggcaa gtgcgtattc
     5221 accaagggca tatgtcgatt cagagagagc aaaccaagca gcagcatagg agggatttac
     5281 atttaaggct tcttgatagg cttcaatcgc cttcatccaa gcagcatttt cttggtactg
     5341 ctgtccgcgc gtataaaaat caagagcggt ttgagcaaag agaaaagaga gagagaaaaa
     5401 acaaaagatt aaaaatcgtt ttttcattca aggtccttat ctttttttat aagttttata
     5461 agatttattt tatgcccttc catatcttgc acaataaaat cgtattcact cgaagaaact
     5521 ttttcatatt ttaccgggat tttgccaaat aagtcaaaaa catagccacc caaggtatcg
     5581 aactcttcgt ttgaaaaatc tgaacctata gcttcattta gatcttcgat gtttactcga
     5641 gcatcacaga gccatatccc ctcacccaag cttaaaatat cttcgccctc gttgtcaaat
     5701 tcatcttgaa tgtcgccaac tatttcttct ataacatctt ccatacaaat aataccggaa
     5761 acaccaccgt attcatcgat agctatggca atatgcacat gtcgcctttt aaattctctc
     5821 aaaagtgtat cgaggcgctt tgattcagga acaaaaaacg cctttcgtat gatttgattc
     5881 aagtcgatag gttctttttt taataaaacc ttgattaaat cttttacgta gagaacgccc
     5941 tccacattat cgattgaatc gctatacacg ggaaagcgtg agtgcccact ttcagcgaca
     6001 atttctaaaa gctcttcttc agaagaatta agtggaacaa agtctacatc tatgcgcggt
     6061 atcatagcct ctttcacaga ggtttgagaa agatcttcga tgccacgaat catatatcgt
     6121 ttctcttcat tcaataattc acgggtaata gtgtcggttt caggacggct ttgcgagcca
     6181 ggctcctttt cagctttttt tgacaataat ttttttataa acctcatgca atatcctcaa
     6241 taatctttat agtataggtt gaaaggagtt tttcttgcaa aacaagcata gcgttttgtg
     6301 cttcctttcc agcttgtata tgatcttctc catggtcata gccgtctaag tgcaaaatac
     6361 catgaataag caagcgtttt aactcttcat cttcagatat atcataactt acggcatttg
     6421 attgaagcat ttctagggaa ataattatgt caccagcaat aaaccatgac tcgccgtctt
     6481 cgtcgacata ttcatcgcct tgctcaaagg agagtatatc tgtcggtccc tcttttcctc
     6541 ggtactcctt attcagcttt gcaatgaagg catttgaaca aaaaagaatg gataactccc
     6601 aatcttctaa ggctaagtca tctaaaacag ctgaaacgaa ggggcttatt ttgtccaacc
     6661 aaactggctt cggaaaagat tcttctacat ctactataat tctttttttc atttttcctt
     6721 aacatctttt tttgattttt ttataagaac agaattagca ttctgtgttt tatgtttggt
     6781 atttttttta tcagagtctt ttgtgccttt ttcatttttc acttccccag tttcttcttc
     6841 atcggggtct ttttgatttg ggtattcgat acgtgaatga tagtaacctg ccaaaatagt
     6901 cacaaaggct ctttgtatgc tcgaaagttc tttaaaagta agcccagaat tatcaagctg
     6961 tcccaattca acctttgtca tcaccagttg tttaataaac ttttcaagtc gtgaaacgga
     7021 aggtttatcg agtgtacggc aggcagcctc aactgtgtcg gcaagcatga caacagcaga
     7081 ttccttcgac tgcgggggag taccaatata cgaaaattcc tctgcgctta cctgtgggtc
     7141 ctgttttttt gcctcggcat aaaagtatga aataaggcta tttccgtggt gttcttcgat
     7201 tatagccaaa acttcctttg gaagccgcaa ttgcgttgcc ttttccaagc ctttttttac
     7261 atgccgccga ataacagaaa cagaaagccg cgggtttagt tcttcgtgtt tattgtttcc
     7321 tgtttgattt tctacaaaat actcaggctg ttcggtttta cctaaatcat gataatatgc
     7381 tccaactcgc gcaagcaggg catttgcacc tatttcgtga caggcacttt cagcgaggct
     7441 tgcaaccatc attgagtgat tataggttcc cggggcgaca atgagcattt ttttcataac
     7501 tggattattt aaatcggata aatccataag acggaaaaca gatgctgtat tgagtagaga
     7561 ttctaaagga gttaacaaac ccaaagcgag gatgcctgaa acaaagccgt taaaaacgtt
     7621 tcctaagata atgctcaggc taaaagtttg tggattatta aaaattattt ttatacagag
     7681 taaaaagata gcgttaaata gccccaataa aaaagaagca aaaatcatat ctatgcgttt
     7741 ttctattctg cgaacaatgc gtattgctgt aattgaagaa gcaactaaaa aaaatgcagg
     7801 aatgggatca aagcctgtaa taaaaagcac agaaaaagcg agcaaaaaaa caaagaagat
     7861 agcattttgc tgcccaaaca gtactgtcaa aagcatcacc gacaatgaag ccggtatgag
     7921 aatgggaagt gcaaaagagg tgtcaaaaaa agtcattttc gacgcaatca ctgttatgag
     7981 ttgaacgaaa acaaagagaa cagctaaaaa aacctgctcc tttgtatcca tttttttgcc
     8041 acaaattttg cttgaaaata aaaaaatcgc aagtgctgca agaagtacat aaaaaataag
     8101 ggcatttgca aaggccctgt agtctatgta tgtaggagag gcagcaagtt tttgtaactt
     8161 ttcatagcct tcctctgtta taggaaagcc tttttttata attttttcat ttttttctac
     8221 agtgattggg tgttcaactg tgggggcaat gtttttagat gaaataatcg ttctatctga
     8281 aatttgtccg agttcgaatt cttgtagctg aaaactaaca atcgtttctt tgcttgcaac
     8341 atcaaaaaaa gcaacagcaa gaacagcaag aaaactaagg gctataatca taaaagaagt
     8401 ggtattttgc tttgcgtgct gccgaaaagt tttccaaaaa tgagagaagg aggaattttc
     8461 ttccaaagta atattgttgt gaatcgtttt tttattcatt cgtgaggata attgtacctt
     8521 ctctattgtc atttcgtcaa gggtttaact ggaatgttcc gtattcatcg acaatttctg
     8581 tttttaagct ctttaaagga ttccatacaa ccgataagga aaaataagga gaaaaatcat
     8641 aataataggg acttgttttt gtaatgagac gtggtgaaaa gctaaactct gacttcaaag
     8701 tccagtcatg taaatcgtgt tctatcttga gagaaagttt ttgcaatttg aaagcagatt
     8761 ccatacgatt tttttcgttc ataaaatcaa atgatttaag caaatctata aaaatatttg
     8821 tttctcctgg cactttaaaa tcatagccat agaagttttg aaagtagcga aatacaagat
     8881 tatttgtcga ttctgatgaa aaggtaattt ttaaaaaatt ataaattgaa aaacttaaag
     8941 agggagaaaa gttaaaatag gagtttgttg gtcgcactaa atccatagta aaacttgttg
     9001 aaagccctgg atttatacta attctatttt tccaaaaaga ccatgaaaga tttgtctttg
     9061 aaaaagaaaa acgtaaatca aagggaataa aatctttttc atcggttgaa agccagccat
     9121 tctcggttaa tttgtacaaa ttcgtgtaaa gcattgaata tctcatggaa aaacttgcat
     9181 aggaaaggcc tgtataaaaa gaagtagaac gcttttcttc aatatcatat ttatagtttt
     9241 gatcgagtat tacagaatta ttgaatactt ttaattgtgc cgtttgaaaa aaaggatcaa
     9301 atcgatattc ttcagttttc aaatcttttt ttatacttgt ttcaaagctt gttgtaagca
     9361 agggaaaaac aaggctgagt tttgtcccat aactttcggt tgtaggagga agggaaagtg
     9421 aaaaggagag tttctgtgca aatatgcctt catctaagga taataaaaag gcaagattgt
     9481 gaacccgtat actttcttta tcccactcag gatagatata tgaccaaaga ggctcctcta
     9541 gggtaccagt aaattctttt tttaataagc gaatatctaa attccaagta agcgaagtgt
     9601 tcttaaagac agcatgagaa aggaagggat ttacactcaa actattttta tttgttatgt
     9661 caaatttgtt ttcagcaagg tcgttcagtt ttattttatc aatatcgact tgattcgtat
     9721 aatattcttc tgagataaag ggatggtctt gtatttgtgg gataaaagaa aaggtattat
     9781 tcatttgaaa aattgcattt ataaaggtcg ccttactcct tagattaagt gggctgcgaa
     9841 gttgaaaata agttgattga agatttgaaa aagatgctgt catatcttca ggggtattta
     9901 tttcagagct atttaaaagc accagtgttt tcaaagaact tgaaaggtca tagcttaaag
     9961 aatatgaaaa aagagtaaag gccgattttg tcagcttcgt agggtttaat tttacggcag
    10021 tcagttcttt tgctacctca tccaaatctt cgctaacaac ttgattcatt atctcttgat
    10081 ttttgtagtc ttcagaaagg ctctctttat tttctatttc tatctcattt ttatctgcct
    10141 cttgtttttt ttcttcggaa aaatcctcag cttttggagc aaagagaaaa atatcttcta
    10201 tatcctgctt tgtgtttttt attttttgtg caaaaggata agaaaacaaa gttccagata
    10261 gcttcatatt tgcatctacc ggttttattt gtgaaggaat ataaaactga cgatttatat
    10321 cgtggataaa ctttgaatct gttttttctg cattttgctt ggtaataaaa tcgactgaag
    10381 aagtgaggtt tgagagcgaa agtgtattta tatagggctt aataaaatct atcttggggc
    10441 tgtagctgag attaagattc cagttaaaac tcgaaagcgc taccttattt ttttgtaaat
    10501 ttggtgtatt catcaatgta ttgtttaaaa aataatttat ccaatccata tcttcgcttc
    10561 gattatgaaa atctgcatta aaataggggt cagaataaaa gggaagagag agagaaaatg
    10621 aaaaagaagg ctttgaaagc gaaagagaaa actcttcgta aaatcgaaaa ggaaggaccg
    10681 ttccaaataa aaaactctgg tcccaaactc tttctcctgt tgcgggattg atagtcggca
    10741 cagggctgtt ggggaaaaag ctccgtgaaa aacctaaacc aaaaccaaag tcaattttct
    10801 tgacaagctg gtttggggaa aaagagcctt ttattccaag atatgcaccg aggtccgaat
    10861 aataatcagc cataaatttc agataatcac tcgaaacatc ctttgcgtcc gtttcaagat
    10921 tatgtaaaaa caaacctctt ctttcctgtt cctttaaagt tgtcggtttt aaaaagttaa
    10981 ataattcttc tattgtttcg gtactttgcg ttttatctgt cttatcagtc ttatccgcct
    11041 gtttttttac ttctaggttt ttcctaccaa caaaatacgt tgttgtttgt aaaaaataac
    11101 cttctcgctc agaataacca aaaacaggat tgaagataag ttcatccttt ggataataaa
    11161 aaaatggaaa ataaaagagc ggcacatttc ccacatacaa aagcgcattg aaaaaagcaa
    11221 attcatttcc cggtaaaagc caaattttcg acgcctttag atgccaatgt ggctcttctt
    11281 catcgctaca aaaggtaagc tttcctttat cgaaggcaat ggtcgaagcc ttatcctttg
    11341 caagcaaatc tgcatagaca atgagttttg aaccttcggg caattgcaag gcagaaagtt
    11401 tttcttgcgc aacacgtaca tcgtcaaaaa ctccttctag agaatcaata ttaaataaaa
    11461 ttgcatcggc atcgatacgc tctgcgggct gagaagcagt cgttttttcc aaaacaaccg
    11521 ctccttctgc atacagcatg ttttgtttgc gattaaaacg cacgagggat gcagaaattt
    11581 ttgttttatc tgttccactt tcaacagata ataaaacatt gcccttaaag acaatggttt
    11641 cttcacctgt tttttcgtcc ttcaagtact cggttttttc tgccttttct atcgtaacag
    11701 tactcgtctt ttgttttgca tcctcttgcg gaaaagcaaa aaaaataaaa ccaagagtac
    11761 aaacaataaa aaaaaggctt tttttcatta ttttccaatc cgattgagtg ctttaagatg
    11821 ctcttttata aaaaatcccg tatgtgattt ttcacaggcg gcaacagctt ctggtgttcc
    11881 gacagtgatt acttctccgc ctcgaaaacc accttcagga cccaggtcaa taatatggtc
    11941 agcctgtaaa ataacatcga ggttgtgctc gatcataaca acggtattgc ccgtatctac
    12001 aagccgttga ataacctcca taagttgctt cacatctgca aaatgtaaac cagttgttgg
    12061 ctcatctaaa atatagagcg tctttccagt cgaacgtttt gaaagctcgt ttgcaagttt
    12121 taccctctgc gcctctccac ccgaaagcgt tagagctgac tgccccagtt ttatataacc
    12181 caaaccgaca gacataaggg tatccatctt acgtgcaata tgaggaatat ggataaaaaa
    12241 ctctgttgcc tcttctatcg tcatatttaa aacatcataa atgttttttc ccttatagcg
    12301 cacttctaag gtttcttgat taaaacgttt tccctgacat acttcacaat taatatatac
    12361 gtcagataaa aaattcattt cgattgtaat tgtacctgca ccttgacagt tttcacagcg
    12421 cccacctttt acattgaagc taaaacgccc cggcttatag ccacgcatct tagcctcggg
    12481 caaactcgca tacaagtcgc gaatgccgtt aaagacaccg acataggttg caggatttga
    12541 tcgaggagtg cgtccgatgg gcccctggtc aatattgata accttatcga tatgttcaag
    12601 ccctgaaatt tttttatgcg cgccaacaga tttagtagat cgcataagcg cattggaaac
    12661 tgcgggataa aaaacatcgc tgataagagt tgattttccc gagccagaaa ctcccgtaac
    12721 acaggtaaag gttccaagag gaatagaaac agagatgttt
//
LOCUS       DAOSSW010000051        12685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_4979_length_12685_cov_6.570309, whole genome shotgun sequence.
ACCESSION   DAOSSW010000051 DAOSSW010000000
VERSION     DAOSSW010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 12685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12685
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4979_length_12685_cov_6.570309"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2139
                     /locus_tag="PKW26_05490"
     CDS             <1..2139
                     /locus_tag="PKW26_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOQ93053.1"
                     /translation="LVPIILTTVFVSILMAKHDERIQYEHTREYAVATARYLETYFTT
                     RTDLINTYAQLYNQGFTSWEEYKDIVEISVTEHIFEKIILAMKDGTYYATGNDGNPYF
                     GGKQTSNNASPNATLSNISSRDYFKYLIHDNTREENRRLISDPVISLSNKKRQILVTQ
                     TIFDSNGKLAGIVAGSITLDFIDTYIEKTNLELKAKFSKDANFIILSNTGAYIYHWIP
                     ELNLHIATIDGVETAISPGIQDENAELQKIASEVIKGKSNTVKYVEPSTKEKFLCTYL
                     PVAGTAYSLLILYPEKIIYEDLMFVIKTNILVIAIIALIVFILSVIFSKSLINPLSIL
                     SGTLKGISMGGADLTKRLSEEGNDVVSDIGKNFNAFSQSLHTIMSGVKNNAHQLQGVS
                     DNLHQNIGHTKNSLLEIDQNVAELSNDAIDLSAAVEETSSTIHQISRNIESLNMQIET
                     QSHSVDDSSASIEEMVANIQAVSNNLNKAKAGFATLRKDSDTGKIAIESVIESINKTV
                     ALSNELLETNEVIGAITNQTNILAMNAAIEAAHAGDAGKGFSVVADEVRKLAEDSAEQ
                     SKKIAEVLKQTVDNINRVLEESNYANKAFDTITSQVTSVLQFIDETDSAMKEQTEGSN
                     QVLEALKKIQNITQEILSGSTEMSSGAGMILTEMERLQNIAVHLNTSSGSMKENIEKI
                     NTAILSVASLSDKNKELGDALQKTSDGFIL"
     gene            complement(2188..4833)
                     /gene="mutS"
                     /locus_tag="PKW26_05495"
     CDS             complement(2188..4833)
                     /gene="mutS"
                     /locus_tag="PKW26_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOQ93054.1"
                     /translation="MSAETPLMKQYFSIKNQYKDEVLFFRLGDFYEMFFDDAIEVAKL
                     LNLTLTQRGDVPMCGVPYHAAKVYIARLLRLGKKIAICEQISLPKDGKGLADRKVVEV
                     ITPGTAIEDEYLEQGSNFLACICLVPGKKQNTKKLQDFISFSFIDISTGFFSATSWPI
                     EDMKDAFARELSRIAAKELLVDERLGDCESLRFVLTQYPSLILSYYPGWHFNVDRSYE
                     RLLAQFEVQNLHGYGLIPESPEIASSGFILEYIQKNYSTDSSDSSDRGSESSSLAHIK
                     DLEVYKDFDSLSIDDSSWRNLEIMQNLRDGSYHYSLLETVSYTKTAMGSRLLHSYLMF
                     PLLSIEKIQQRQSRVQFFYDDSFLRSAIQLHLREIFDIERLTSRIVMQKAHAKDLVAL
                     KKSLMAWYKIRELLKEKEFISFDTTKAKEIIDTIDKAILDEPSTSFTEGSLIKTAWSK
                     ELDELHLLQKNFKSILKDYTEEEKNATGIQNLKVRYNRNMGYYIEVTKGKLSSVPSHF
                     ILRRALVNADRYTTERLQELETKLLSADETIIALEQSLFFELRENLAKEHSYLFSLAK
                     DIALIDVSACLAEAASEQNWICPEILATRELEIIEGRHPVVEFHMQDGSFIPNSLELN
                     EKPFALITGPNMAGKSTYLRQTALIVLLAQIGSFVPATKARIGIVDKIFCRVGSSDNL
                     ARGESSFLVEMTETSQIVRGATKNSLVIMDEVGRGTSTEDGLSLAWAISEYILNKIQC
                     KTLFATHYHELTRLTHSLLQLLCLEVLETEEKIIFLKKIKEGAAENSYGIHVAKLAGL
                     PLSIIDRANEILSSLQKTAPYTALEIPEPQEKKETKREVQNFLFSQEELVIDAILSLD
                     PNNLTPLEALQAIAQWKKNLTAKDS"
     gene            4874..5338
                     /locus_tag="PKW26_05500"
     CDS             4874..5338
                     /locus_tag="PKW26_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOQ93055.1"
                     /translation="MISFTHKDGVLYIIASGHVKAHDCGPIKDFATKHITEKDIITDV
                     YLDMKECNYMDSTFIGVIAGINKQLRKKWKKSIHLQNTNKACMDLLDDMSLSPLLIFL
                     SEAISFPTEATKVDDNILISKIDIAEAHEELTELSEDNKKKFGLLAKILRES"
     gene            complement(5343..5861)
                     /locus_tag="PKW26_05505"
     CDS             complement(5343..5861)
                     /locus_tag="PKW26_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOQ93056.1"
                     /translation="MKKNAILLVFVFIGFCAFSQQIPRIGVVDTSRVFQTYFRDSVAV
                     RNYENKKAEFQNEINKKTEELKQLLQQKIQYEENENFVAALRLEAEITKKTDYLSSYT
                     QAKQIELDSIRNRLENSDSFYKSLYEVIGQIAEGEGYSLILSLQQAQNILWYSPSVDI
                     TDKIITQLAKKQ"
     gene            complement(5871..8270)
                     /gene="bamA"
                     /locus_tag="PKW26_05510"
     CDS             complement(5871..8270)
                     /gene="bamA"
                     /locus_tag="PKW26_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOQ93057.1"
                     /translation="MYRKHLVFFTVVLFCVSFFAFSQDSEWYYNKTIKRIYFEGLVSV
                     SNTDVSAVTNQFIGKEFTDAVFEQLLSSLFSLDYFKDIETDIVPLDAQGSTLMVRFTV
                     VEYPLVSKVSFSGNSQIKNSELKDALSIKEKTIFNEAKVLIDERALRDLYLNKGYTNI
                     RVNSQTLEQTDGSIQVVYTLTEGKQTIVTGISFEGNATFAQKSLKKEMKQKEKTLFEK
                     GIFQEAYVELDKQNLINFYNERGYIEASIVDVKREVSFNETDNRDELSLHYVIREGSQ
                     YVYKGTSITGNKLFSTEELLAKIQLKDGDVFNVIKFSSGLRAIYDTYVEKGYTSNYFA
                     PQEYKNVEAKTIAYTIEIIEKPRSYIENIIIRGNEKTKEEVILREIPLESGDVFSQTK
                     VYNGIRNLYNTQYFSNILPDMQQGSEENLIDLIFEVKEQSTTSLEFGVTFSGVADPDT
                     PPISFFSKLTDSNFMGTGRTIGAELTIADGNYTIGGTFLENWLFGKPISLFVQANLNY
                     KTLTTFQETFFPENTNIYVMDYNELSTNLNFGLGRRWYPSFAILSLNSGFTINLFKNF
                     YDAESFVPLEEKVKSAYDMVGVTNSFYISLSLDDRDIFYDASKGWFASQKLSWTGLIP
                     AVETEFFLRSDTKFEGYLTLLDKPVTDIWNLKFVFFAYTGLSFLVPTSNTSIANDSKL
                     LVDGMFNGRGWNTVRNNRGNALWSTNLELRFPVAPGMLSLDAFYDIAVVKDSLQDFAH
                     LSQDDFYYSFGAGLRISVPQFPLKFLWAFPYKYENGNFQWKNNTQFPIEFVLSFNIVN
                     R"
     gene            complement(8287..12669)
                     /locus_tag="PKW26_05515"
     CDS             complement(8287..12669)
                     /locus_tag="PKW26_05515"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOQ93058.1"
                     /translation="MSKKIENYKDSLVFALEKELGIIISYEKMSPSVLNSLKISNITV
                     FDTKDNSALVQIPSIRVYLSLLSLIRGDFDSIVSSITINGLHIEYDDFLDSHILENIQ
                     NSIFFNTSTEEIEAKKTFAFPFNVTCKALSIHFHNEELEAFVYFKELAFKSSPTKKTT
                     SYKAEGRMQVEIFKNDLSRLGKTETTFRAQGNFSSLLDSSFLQLRLSKLDNKMFSLQA
                     FDLAIIHEKESLRFSLLQNAGAIALKATYTMTTKNIDASLKMLDFYPIKMIHFKENQA
                     FFSNLNALRFSGDFSSSILNLDFSSLHYFYDAIVQVPASLFPGSLELALRGEGSLSKL
                     FVEKLDAKGKMLDFDFAGSYIFETMKPEGFATVHTLHLPSDNAISFELYLDATRDGFL
                     FFIPQMEVGEKNFTALELELIPRKNSLDFLFYAYDYSHYEYMENAEISFSGSLLFEKK
                     PFLQMNLAVSNLFLDSIFSVLEYSLSENNDVFASLKNNFSTFLTSTEIYITTDFTQFS
                     YNVPYAIIANTVKDKELVVFSLGGNESSLSLTQFDLLFANQSLESTANFDFLAGFSDA
                     FFTMSLVFNSLPYEIQGGIVDKKNIYISGSYGLDALLHLASDSLLSGSLAINAMPIAV
                     KNSFYALSLDTDFYYNSFSDWYATIASFDLSQTGGENRLSPQIFMAGFVDTYGAAMHS
                     VLYTDTISSLEGHLLSSWSFSEGILSRSDLHLELQDLSEQNSKKEAYSLSFALTNPSE
                     LALSTADFFDDTYITAEIAFENSPVERFLTQQNTANQFSADMHVLGTIKKPSISFSLY
                     NTFLDFGGMRTELTAHCLFENGNITIPDASIKTDTFSLQNIQAIFDTQAFSGSINGNA
                     SIQNLVHAKTEKAFIIDIFSPFPQKEIKEKTFLSFLEIPETFSLNLRIPEAKKNVQGR
                     RDDFNLSFIRSPSRFDIQGGFGPASSIISTPSIQGYYTDLGDILVLLDERLPLSLLAY
                     GQIKENLINITFSDLVLDASHFTWFLNFGEFKILEGFGKGNFSIGGYVQDPEFSGIVE
                     VVDFACEVPHFIPGIIRAETGTILAEDKVIQMPQILGLTKKGSCVVDVALHFDKWDVE
                     RVEIDLETPKNEKVLVAADFGLLNIQGNASCDLHLEIYQELFSITGFIDAEDAQAKIY
                     PAELISQNMEESSLDIYTDLKIRVGKRSQVSLFLFRQINPIVRSLISPNTQLRFVMDS
                     RKNLSYLEGDIVLKGGEVNIGRSFYIKEGRLSFEESQGSFDPLVNLIAEMRERDSTGD
                     FVKITLSALNQRLSDFNYTLSSSPPKSEVEIMSLLGKTLVYDPSGSVGQDMLNVLGAG
                     LDSFVQSTIMREAEDRLRDFFKLDILTLRMSVAQNLIKLYSEDNSGKGINPGNFLDDS
                     SVYIGKYFGDVLYGDFLLHLSYDESKGNPNVFGSGLSFQPELGFEIEAPFATIRWGLA
                     PDVESIKNLWVPFTSLSLSWKILF"
BASE COUNT         4150 a         2268 c         2277 g         3990 t
ORIGIN      
        1 ttagttccta ttattttaac tactgttttt gtatctattc tcatggcaaa gcatgatgag
       61 cgtattcaat acgaacatac aagagagtat gctgttgcga cggcgcgcta tttggagacc
      121 tattttacga cccgtacaga tctcatcaac acttatgcac agctttataa tcaagggttt
      181 acctcttggg aagagtataa agatattgta gaaatatctg ttacagaaca tatttttgaa
      241 aaaattattt tagctatgaa agatgggacc tactatgcga ctggaaatga tggcaatccc
      301 tattttggtg ggaaacaaac gtctaataac gcaagcccaa atgccacact ttcaaatatt
      361 tcttctcgag actatttcaa atatctcata catgacaata ctagggaaga aaaccgtagg
      421 ctcatatcgg acccagttat ttcactaagc aataaaaaaa gacaaattct cgtaacgcaa
      481 acgatttttg acagcaatgg aaaacttgct ggaattgttg caggttctat tacgctcgat
      541 tttatcgata cctatataga aaaaacaaat cttgaactga aagcaaagtt tagcaaggat
      601 gcaaatttta ttatcctttc aaatacaggt gcctatattt atcactggat acctgaactc
      661 aatttacata ttgcaactat agatggtgta gaaacagcta tttctcctgg aatacaagat
      721 gaaaatgcgg aattacaaaa aatagcttct gaagttataa aaggaaaatc aaatacggtt
      781 aaatatgtcg agccttcaac aaaagaaaaa tttttatgta cctatctacc tgttgcagga
      841 actgcatact cacttttaat tctttatcct gaaaaaatta tttatgaaga cctaatgttt
      901 gtaatcaaga caaatattct agtcatagct atcattgcgc ttatcgtatt tatcctctcg
      961 gtcattttta gtaagtctct catcaatcca ctttctattc tttctggaac cctaaaaggt
     1021 atctccatgg gtggagcaga tttaacaaaa cgtttgagtg aagaaggtaa cgacgttgtc
     1081 tccgatatcg gaaaaaactt caatgcattt tctcaatcct tgcatactat catgagtgga
     1141 gtaaaaaaca atgcgcatca actgcagggt gtaagtgata atctacatca aaatataggg
     1201 cacacaaaaa actcgctttt agaaatagat caaaacgtag ctgagctctc caatgatgca
     1261 atagatttaa gcgctgcggt tgaagaaact tcatcgacga ttcatcaaat ctcacgcaat
     1321 atcgaaagtt taaatatgca gatcgaaaca caatctcata gcgtagatga ttcttcggct
     1381 tctatagaag aaatggttgc aaacattcaa gcggtttcaa ataacttaaa caaggcgaag
     1441 gctggttttg caactttacg aaaagatagt gatacaggaa aaattgcaat tgagtcggtt
     1501 atagaaagta taaataaaac ggttgcgctt tcaaatgaac ttcttgaaac aaacgaagtg
     1561 atcggtgcta ttactaacca aacaaatatt cttgcgatga atgctgcaat tgaagctgct
     1621 catgcagggg atgcaggaaa gggtttttct gttgttgccg atgaggttcg aaagcttgct
     1681 gaagattcgg ctgagcaatc caaaaaaatt gcagaagtat taaagcaaac tgttgataat
     1741 attaatcgcg tgcttgaaga atcaaattat gcaaataagg cctttgacac aatcacgagt
     1801 caagttactt ctgtacttca gtttatcgat gaaacagact ccgcaatgaa ggagcaaaca
     1861 gaaggctcga accaagtatt agaggcgctt aaaaaaatac aaaatattac gcaagaaatt
     1921 ctttctgggt cgactgaaat gtcgagtggt gctggtatga ttttaaccga aatggaaagg
     1981 ctacaaaaca tagcggtaca cttaaacaca agctctggct cgatgaaaga aaatatagaa
     2041 aaaataaata cagcgatact atcggttgca tctttatctg ataaaaacaa agaactcggt
     2101 gatgcactac aaaaaacatc cgatgggttt attttataat acgattgcat acaagggtag
     2161 ataaaaatct aagtacaact caggttttta gctatccttc gcagtcaaat tttttttcca
     2221 ttgtgcaatc gcttgcaaag cttcaagcgg ggtaagatta tttggatcaa gggagaggat
     2281 tgcatctata acaagttctt cttggctaaa taaaaagttt tgcacttctc gctttgtctc
     2341 ttttttttct tgtggctcag gaatttccaa tgcggtatag ggtgctgttt tttgcaagga
     2401 gctaagtatt tcatttgctc tgtcaataat ggacaaagga agtcctgcaa gttttgcaac
     2461 atgtataccg tacgaatttt ctgcagctcc ttcttttatt tttttcaaaa agataatttt
     2521 ttcttctgtt tcaagaacct ctaggcaaag aagttgcaag agtgaatgtg tcagtcttgt
     2581 tagctcatga tagtgggttg caaagagtgt tttgcattgt attttattta agatatattc
     2641 agaaatagcc caggcaagag aaagtccatc ttcggttgag gtaccgcgtc ctacttcatc
     2701 cattataact aaactatttt ttgtagctcc ccgaacgatt tggcttgtct ctgtcatctc
     2761 taccaaaaag cttgactcgc ctcgtgcaag gttatcagag gaaccaactc gacaaaaaat
     2821 tttatccacg atgcctatgc gggccttagt cgcgggaaca aaggagccaa tttgtgcaag
     2881 caaaacaatg agtgcggttt gtctcaaata cgtactcttc cctgccatgt ttggacctgt
     2941 gatgagtgca aaaggttttt cgtttaactc gagagagttt ggtataaagc ttccatcctg
     3001 catatgaaat tctaccacag ggtggcgtcc ttcgattatt tccaattccc ttgttgcaag
     3061 aatctcgggg caaatccagt tttgctcgct tgctgcttcg gctaagcaag ctgaaacgtc
     3121 tatgagcgca atatcttttg caagtgaaaa taaataacta tgctcctttg caaggttttc
     3181 acgtagctca aaaaacaaac tttgttcaag ggcgataatt gtttcatcag cacttaaaag
     3241 ttttgtctca agttcttgta agcgctctgt tgtatagcga tcagcattta ccaatgcacg
     3301 ccgtaaaata aaatggcttg gaaccgatga aagctttccc tttgttactt ctatatagta
     3361 acccatattt ctgttatagc gtacttttag attttgtatg cccgttgcat ttttttcttc
     3421 ttcggtgtag tcttttaaaa ttgacttaaa atttttttgt agaagatgca actcatcaag
     3481 ctcttttgac caagctgttt ttataagaga gccttcagta aaactggtac tcggttcatc
     3541 gagaattgct ttatcgattg tgtcaattat ttcttttgcc ttggtcgtgt cgaatgaaat
     3601 aaattctttt tctttcagaa gctctcgtat cttataccaa gccataagac tttttttcaa
     3661 agcaacgagg tcctttgcat gtgctttttg cataacaatg cgagaagtta agcgttctat
     3721 atcaaaaatt tctctcaaat gtaattgaat agcggagcgt aaaaaagaat catcgtaaaa
     3781 aaactgtact cgactttgcc tctgttgtat tttttctatg cttaaaagag gaaacatcaa
     3841 atagctatgc aaaagtctcg agcccattgc ggtttttgta tacgagactg tttccagcaa
     3901 actatagtga taggagccat cgcgtaggtt ttgcattatt tccaagttgc gccaagaaga
     3961 atcatctatc gaaagagagt caaagtcttt atagacttct aaatctttta tatgtgcgag
     4021 ggaactcgat tcagatcctc tgtctgagct atctgaacta tctgtgctat aatttttttg
     4081 tatatactca agaataaaac cgctcgaagc tatttctggg gattcaggaa tcaagccgta
     4141 tccatggaga ttttgcactt cgaattgtgc taaaaggcgt tcgtatgaac gatcaacatt
     4201 aaaatgccat ccgggataat aacttaaaat cagtgagggg tattgtgtta atacaaagcg
     4261 aagtgattca caatcgccta agcgctcatc aactaacaat tcttttgctg ctatgcgaga
     4321 gagttcccga gcaaaggcgt ctttcatatc ttcgatgggc catgaagttg ctgagaaaaa
     4381 tcctgttgaa atatctataa aagaaaaaga aataaaatcc tgtaacttct ttgtattttg
     4441 tttttttccc ggcacaagac aaatacaagc gagaaaattg cttccttgtt ctaagtattc
     4501 atcttcgatt gcggttcctg gtgttataac ttcaacaact tttcgatctg ctaggccctt
     4561 cccatctttt ggtaagctta tttgctcaca aatagcaatt ttctttccaa gtcgtagaag
     4621 acgtgctata tagacttttg cagcatgata gggcacacca cacatgggga catcgcctct
     4681 ttgcgtaagc gttaggttta agagttttgc aacttctata gcatcgtcaa aaaacatttc
     4741 ataaaaatca ccgaggcgaa aaaaaagaac ctcatcttta tattgatttt ttatagaaaa
     4801 atactgtttc attaaggggg tttctgcact cattttactt gcattataag atttttaagg
     4861 gtaaaataca agcatgatta gttttactca caaagatggt gttttgtata taatcgcaag
     4921 tggacatgtc aaagcacatg actgtggtcc catcaaggac tttgcaacaa aacacattac
     4981 agaaaaagat ataatcacgg atgtatatct tgatatgaaa gaatgtaatt atatggactc
     5041 aacctttata ggtgtcattg caggtataaa caaacaatta cgtaaaaaat ggaaaaaatc
     5101 catacatctg caaaatacaa ataaagcctg catggatcta ctcgacgata tgagcctcag
     5161 tcccctactc atctttttaa gtgaagcgat ttcctttcca actgaagcta caaaagttga
     5221 tgataatatt ttaatatcga agattgacat tgccgaagca catgaagaac ttacagaact
     5281 ctccgaagac aataaaaaaa agtttgggct tctcgcaaaa attctgcgtg aaagctaatt
     5341 ttttattgct tctttgctaa ctgtgtaatt attttgtcgg tgatgtctac gctggggcta
     5401 taccataaaa tattttgtgc ttgttgtaaa ctcaaaataa ggctatagcc ctcgccttca
     5461 gctatctgtc caataacttc atacaaggac ttataaaaac tatctgaatt ttcaagtcta
     5521 ttgcgtatgg agtcgagttc gatttgcttt gcttgagtat aggagcttag ataatctgtt
     5581 ttctttgtta tctcggcttc caatctgagt gcagctacaa agttctcatt ttcttcatat
     5641 tgtatttttt gttgtaagag ctgtttgagc tcctcagttt ttttatttat ttcattttga
     5701 aattctgctt ttttattttc gtagtttcga accgcaaccg agtctctaaa ataggtttga
     5761 aatacacgag atgtatcaac tacaccgatt cggggaattt gttgcgagaa agcacaaaaa
     5821 ccaataaaaa caaaaacaag tagtatggca ttttttttca tagttcttcc ttatctattt
     5881 acaatattaa atgaaagcac aaattctata gggaattgtg tattgttttt ccactggaaa
     5941 ttaccatttt catacttata gggaaaggcc caaagaaatt taagggggaa ttgcggaact
     6001 gaaatacgca agccagctcc aaaactatag taaaaatcat cttgcgaaag atgcgcaaag
     6061 tcttgaagag aatccttaac aacagctata tcgtaaaagg catcgagtga aagcattcca
     6121 ggggcaacag gaaaacgcaa ttcaaggttt gtgctccaga gggcatttcc acgattattg
     6181 cgtacagtat tccagcccct tccattaaac attccgtcaa ccagcagttt gctatcgttt
     6241 gcaatcgagg tatttgacgt gggaacaagg aaagagaggc cagtgtaggc aaaaaacaca
     6301 aattttaagt tccatatatc tgtaactggc ttatcgagga gcgtaagata gccttcaaac
     6361 tttgtatctg atcgaagaaa aaattcagtt tcaacagcag gaatcaaacc agtccacgaa
     6421 agtttttgac ttgcaaacca gccctttgaa gcgtcataaa aaatatctct atcatcgagt
     6481 gaaagggaga tataaaatga atttgtaaca ccgaccatat cgtaggcaga ttttactttt
     6541 tcttctaaag gaacaaaact ttcggcatca taaaagtttt tgaataaatt tattgtaaat
     6601 ccggaattaa gcgaaagaat tgcaaaactt ggataccagc ggcgtcctaa accaaaatta
     6661 aggtttgtag aaagttcatt ataatccata acatagatat ttgtattttc tgggaaaaat
     6721 gtttcttgaa aagtagtaag cgttttataa ttcaaatttg cttgaacaaa caaggatata
     6781 ggcttaccaa agagccaatt ttctaaaaat gtaccaccaa ttgtataatt cccatcagca
     6841 atagttagtt cagcaccaat agttcgtcct gttcccataa aatttgaatc ggtcaatttt
     6901 gagaaaaaag atatgggtgg ggtatctggg tctgcaacac cagaaaaagt taccccaaat
     6961 tccagagaag ttgtcgattg ttcttttact tcaaaaatta aatctataag attttcttca
     7021 gaaccttgtt gcatgtcagg taagatattt gaaaaatact gggtattgta caaatttcgt
     7081 ataccgttat acaccttcgt ttgagaaaaa acatcgccac tttcaagagg tatttcacgt
     7141 aaaataactt cttcctttgt tttttcattt cctcgtataa taatattttc gatatatgaa
     7201 cgtggttttt caattatttc aatagtataa gcaattgttt ttgcttcaac gtttttgtat
     7261 tcttgcggtg caaaataatt tgaagtatag cccttttcaa cataggtatc atagattgca
     7321 cgcaaaccag agctaaattt tataacattg aagacgtctc catcttttag ttgtattttg
     7381 gctaagagtt cttcagtgga aaaaagttta tttcccgtta tgcttgtacc cttatacaca
     7441 tactgactgc cttctcgtat tacataatgt aagcttaatt catctcgatt atcggtctcg
     7501 ttaaaagaaa cctctctttt aacatcaaca atacttgctt ctatataacc gcgctcatta
     7561 taaaaattaa taagattttg tttatcgagc tcgacatagg cttcttgaaa aatacccttt
     7621 tcaaagagtg ttttttcttt ctgtttcatt tcttttttta atgatttttg tgcaaatgta
     7681 gcatttcctt caaaagaaat acctgttaca atggtctgtt taccctcagt aagagtataa
     7741 accacctgaa tagagccatc tgtttgttca agtgtttgag aattaacacg aatgtttgta
     7801 taccctttat tcaaatacag gtctctgaga gcacgctcat ctatgagcac ctttgcctca
     7861 ttaaaaatag ttttctcttt aattgacaag gcatctttta gttcagagtt ttttatttgg
     7921 ctatttccac taaagctcac cttagacaca aggggatatt caactacagt aaagcgaacc
     7981 atgagagtcg agccttgtgc atcaagggga acaatgtccg tttcaatatc tttgaaataa
     8041 tctaaggaaa acaatgaaga taaaagctgt tcaaaaacag catctgtaaa ttctttgcct
     8101 ataaactgat ttgtaactgc tgaaacatca gtgttactaa cactcactaa gccttcaaaa
     8161 taaattcttt ttatcgtttt gttataatac cattcagaat cttgtgaaaa agcaaaaaaa
     8221 ctaacacaaa ataatacgac agtaaaaaac acaagatgtt tacgatacat atatcctcca
     8281 acctttttaa aacagtattt tccaagacaa acttagggaa gtaaaaggta cccacaagtt
     8341 ttttatagat tcaacatcgg gagcaagccc ccaacgaatt gttgcaaatg gagcttctat
     8401 ttcaaagcca agttcgggct gaaatgaaag cccgcttcca aaaacatttg ggtttccttt
     8461 tgattcatca tacgataaat gtaatagaaa gtctccatag agaacatcgc caaagtattt
     8521 acctatgtaa acactcgaat catctaaaaa gttaccggga tttattcctt ttccagaatt
     8581 atcttcagaa tataacttaa taagattttg cgcaactgac atccgtagtg ttaatatatc
     8641 aagtttaaag aaatcacgca atcggtcttc agcctccctc ataatggtgc tttgtacaaa
     8701 gctatctaaa ccagctccga gtacattgag catatcttgc ccaacagagc cggagggatc
     8761 atagacaagt gttttaccta ataagctcat gatttcaact tcagattttg gtggagatga
     8821 agataaggta taattaaagt cgctcaatcg ttgatttagt gcactgagcg taatcttaac
     8881 aaagtcccct gtactatccc gctccctcat ttctgcaata aggtttacaa gcggatcgaa
     8941 agaaccttgg ctttcttcaa aagaaagtct tccttcctta atataaaaac ttcgcccaat
     9001 gtttacctct ccgcctttta gaacaatatc tccttctaaa tacgatagat ttttccttga
     9061 gtccataaca aagcgcagct gggtatttgg gctgataagg gaacgcacaa tagggtttat
     9121 ttgccgaaat aaaaataagg atacttgcga ccgcttgcca actcgtattt ttaaatcagt
     9181 atatatatca agagaggact cttccatatt ttggcttatc aattcggcgg ggtaaatctt
     9241 tgcttgagcg tcttcggcat ctataaaacc ggtaattgaa aatagttctt gatatatttc
     9301 taaatgtagg tcgcagcttg catttccttg tatattaagc aaaccaaaat ctgcagcaac
     9361 gagcactttt tcattttttg gtgtttctaa atcaatttca acccgttcaa catcccattt
     9421 atcaaaatgc agggctacat cgaccacaca agaacctttt tttgttagac caaggatttg
     9481 cggcatctga ataactttgt cttctgcgag aattgttcct gtttctgctc gaatgatacc
     9541 gggaataaaa tgtgggactt cgcatgcaaa gtctacaact tcaactattc ctgaaaactc
     9601 tgggtcttgt acataaccac cgatagaaaa gtttccctta ccgaaacctt ctaggatttt
     9661 aaattcacca aaatttaaaa accaggtaaa atgagaggca tctaaaacga ggtctgaaaa
     9721 agtaatattt attaggtttt cttttatctg cccataagct aataggctta aaggtaaacg
     9781 ctcatctaaa agtactaata tatctccgag gtctgtataa tagccctgta tagaaggcgt
     9841 agaaataata cttgatgctg ggccaaaacc gccttggata tcaaaacgtg atggagaacg
     9901 tataaacgaa agattgaagt catcacgcct tccttgtaca tttttttttg cttctggtat
     9961 acgtagattc agagaaaatg tttcaggtat ttctagaaag gagagaaagg ttttctcttt
    10021 tatttctttt tggggaaaag gtgaaaaaat atctataata aatgcttttt cagtttttgc
    10081 atgtacgaga ttctgaatac tcgcatttcc attgatagag cctgagaaag cttgcgtatc
    10141 aaaaatcgcc tgaatgtttt gaaggctaaa agtatcggtt tttatacttg catctggtat
    10201 agtgatgttg ccgttttcaa aaagacaatg agctgtcagt tctgtacgca tgcctccaaa
    10261 gtcaagaaaa gtgttataga gagaaaaaga aatacttggc ttttttattg tgcccaaaac
    10321 gtgcatgtcg gcactaaact gatttgctgt attttgttgg gttaagaaac gttctacagg
    10381 actgttttca aaggctattt ctgcggtaat gtatgtgtcg tcaaaaaaat cagctgtgct
    10441 tagtgctagt tctgacggat ttgttaaggc aaaagagagg ctataagctt ctttttttga
    10501 gttttgctcg cttaggtctt gtagttcaag atgtaaatct gatcgagata gaatgccctc
    10561 agaaaaagac caagagctaa gtaaatgccc ttcaagtgat gatatagtat ctgtatacaa
    10621 aacagaatgc attgccgctc cgtaggtgtc aacaaaaccg gccatgaata tctgcggaga
    10681 aagtctattc tcgccccctg tttgactcaa gtcaaagctt gctatcgtag cataccaatc
    10741 tgaaaaagaa ttataataaa aatctgtatc gagtgaaaga gcgtaaaaag aatttttcac
    10801 agcaataggc atcgcattga tggcaagact tccgcttaaa agagaatcag aggcaaggtg
    10861 taagagtgcg tctaacccat aactacctga aatatagata ttttttttat ctactatgcc
    10921 gccttgaatc tcatatggta aggaattaaa aacaagggac attgtaaaaa aagcatcaga
    10981 aaaacccgct aagaaatcaa agtttgccgt actttctaga ctttgattgg caaaaagcaa
    11041 atcaaattga gtgagcgaaa gggagctttc attacctcct aatgaaaaaa caacgagttc
    11101 tttgtctttt accgtgtttg caataatagc atagggcaca ttataactaa actgagtaaa
    11161 atccgttgtg atatatattt ccgttgaggt aagaaaggta gaaaaattgt tttttaaaga
    11221 agcaaataca tcattatttt ctgagaggct gtattctaga accgaaaaaa ttgaatcgag
    11281 aaataaattt gaaacagcaa ggttcatttg taaaaatggt tttttttcaa acaaaaggct
    11341 tccactaaaa ctaatttctg cattttccat atattcatag tgcgagtaat cataggcata
    11401 aaacaaaaaa tctaaggaat tttttctagg aatgagttct agttctaagg cagtaaaatt
    11461 tttttcacct acttccattt gcggaataaa aaataaaaaa ccatcgcgtg ttgcatcaag
    11521 atagagttca aatgatatgg cattatcact gggcaagtga agtgtatgaa cggtggcaaa
    11581 accttcgggt ttcattgtct caaaaatgta ggaaccggca aagtcaaaat caagcatttt
    11641 tccttttgca tctagctttt ccacaaaaag ctttgaaaga ctcccctcac ctcgaagagc
    11701 aagttctaag ctaccaggaa aaagagatgc tggtacttgc acaatggcat cataaaagta
    11761 atgtaagctt gaaaaatcaa ggtttaaaat agaggatgaa aaatcaccag aaaaacgaag
    11821 cgcattcaaa ttagaaaaga aagcttgatt ttctttaaaa tgaatcattt ttatagggta
    11881 aaaatcaagc attttcaagg atgcatcgat attttttgta gtcatagtat aggtcgcttt
    11941 taaggcaatg gcccctgcat tttgtaaaag agaaaaacgt aggctttctt tttcatgaat
    12001 aatcgctaaa tcaaaggctt gtaaagaaaa cattttgttg tctagttttg aaagacgaag
    12061 ttgtaaaaat gaagagtcta agagagatga aaaattgcct tgagctctaa aagtagtttc
    12121 agttttgcct aaacgagata aatcattttt aaaaatctca acttgcattc taccttctgc
    12181 tttataactc gttgtttttt tagtaggact tgatttaaag gcaagttctt taaagtaaac
    12241 aaaggcttcg agttcttcat tatgaaaatg aatagaaaga gccttgcaag taacattgaa
    12301 gggaaaagca aatgtttttt tagcctcaat ttcttcagtg cttgtattaa aaaaaataga
    12361 attttgtata ttttccagga tatggctatc caaaaaatca tcatactcga tatgtaagcc
    12421 atttatagta atagaggaaa caatactatc aaaatcgcct cgtattaaag aaagaagaga
    12481 aagatacaca cgaattgacg gaatctgtac cagagcacta ttgtcttttg tgtcaaagac
    12541 agtgatattt gaaattttta ggctattcaa aacagaagga gacatttttt catagcttat
    12601 aatgatacca agctcttttt ctagcgcaaa gacaaggctg tccttatagt tttcaatttt
    12661 tttactcatc ctacttataa taggt
//
LOCUS       DAOSSW010000052        12163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_5235_length_12163_cov_5.766601, whole genome shotgun sequence.
ACCESSION   DAOSSW010000052 DAOSSW010000000
VERSION     DAOSSW010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5235_length_12163_cov_5.766601"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PKW26_05520"
     CDS             <1..821
                     /locus_tag="PKW26_05520"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOQ93059.1"
                     /translation="KVLSDLDAKIELNNKINRELEAMAKTLYDYWFVQFDFPTPPSST
                     SGIVGKPYKSSGGKMVYNAELKREIPEGWEVGTLLDIATFTNGLACQKYRPINKEFLP
                     VIKIREMREGFTKDTEKVRPDISENLIVNNGDVLFSWSASLEVIIWTGGKGGLNQHIF
                     KVMSKKYPRSFYYFQLIWYLQHFKMIAELRKTTMGHITQEHLKQSRIVVPPLDLIKQL
                     DKQLNPIQEKQVLLHKQNQQLSSLRDWLLPMLMNGQVASRASATGESRASATEE"
     gene            1037..1669
                     /locus_tag="PKW26_05525"
     CDS             1037..1669
                     /locus_tag="PKW26_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlbB"
                     /protein_id="HOQ93060.1"
                     /translation="MASGKTIAKTILLLLLILILILGGMIWLDFLGVTQTKSRFAPLY
                     RMLGLEPQTMSSISSIPDIAIVNLDNDRLAKRLEALTIRSEELDKREEDISKKEQENL
                     QILAELDNLRESQEEREKTFTSMTKTFDDREENIAKNAKNLSSMPPKNAVDILVAMED
                     QDIIDVLRKADQIAAEEGTSSLVAYWLSLMPADRVAQIQRKMTSKPLQLP"
     gene            1893..3311
                     /locus_tag="PKW26_05530"
     CDS             1893..3311
                     /locus_tag="PKW26_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOQ93061.1"
                     /translation="MLAYNLHLEKTAYMPDLSNQGLKRQTQGDSSFFDLLQTEIKAKQ
                     SEETKEPRDGQKTATESQKTQSKTDENLAEKDLPVAKNNESESKATQAQAQVKEFEED
                     SLFIETDLQEKAIADSEFIDLNALDEKISPKNVNESLSLENESKMPLVLEDLNEVSEE
                     LVSLNDSQLAYLLKEARSEDEVDISQDSHIHASLESLFFDESSSSLKDISKDDEEALL
                     QRLLTGAVDLSVLQDDFSDAALEVEDSKTLGVQNAEKMLFGSENAIISVVDERTSSKE
                     AKKTDQFVKSIQFETDGKTANVVMSLPSQAEAVGFGSEKESASRFQTMLANEIKNSAT
                     DLVKTGSIILKDNNSGLINLFLKPEALGDVKIRLNISDTLISAKIIVDSQEALNAFKD
                     SIGALKQAFEQGGFDTGSFDLSWSGQGGSGQEKNEHNAKAHFYSDALPELVQDELESN
                     ERILALMKNAIQYGTSIVNVIA"
     gene            3327..3767
                     /gene="flgD"
                     /locus_tag="PKW26_05535"
     CDS             3327..3767
                     /gene="flgD"
                     /locus_tag="PKW26_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HOQ93062.1"
                     /translation="MDFTATMSATDQLYAKNTVDAFNKTLTVNGRQQSNQLGKDDFLK
                     LLLAQLANQDPTSPMENTEFIAQMAQFSSLEQMTNMSTEFSRLANMLHSNEAINIIGK
                     RVDIEEGALSLSGHVDGAVLGVNPQVEINGMLYSMEAIKRVYSE"
     gene            3783..5174
                     /gene="flgE"
                     /locus_tag="PKW26_05540"
     CDS             3783..5174
                     /gene="flgE"
                     /locus_tag="PKW26_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HOQ93063.1"
                     /translation="MMRSLYAGVSGMQNHQTRLDVIGNNVSNVNTTGFKRGRVNFQDM
                     ISQQLSGAAKPTTEVGGVNPKEVGLGMMIASIDTIFTQGNLQSTGVATDVAIQGNGFF
                     VLKNGEQSFYSRAGAFGLDNEGTLVNPANGFRVQGWMAQEMDGEMVLNTASNAQDLII
                     PVGTKDPAKATENVNFACNLNKNTPEILEGASPADIAKGTWATEFKVYDSFGNDHNLT
                     VSFTRVVGNPNQWEGRVIVDPENAEETQTRVGLGTTDGVEDFFLVNFDNTGTLLSVTD
                     SAGNNTNPEGEILLQTSFTVMDSNPDADGNPTRQTLNINLGTIGSQKNTITQSASQSS
                     TKAFYQDGYTMGYLDNFKIDSSGIITGVYSNGTNRTIGQIALATFTNQGGLEKVGDNN
                     FVESNNSGLANIGTSGIAGKGSLLAGALEMSNVDLTDQMTDMIVTQRGFQANSKTIQT
                     ADTLLETVMSLKR"
     gene            5227..5424
                     /locus_tag="PKW26_05545"
     CDS             5227..5424
                     /locus_tag="PKW26_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HOQ93064.1"
                     /translation="MIEVTRLDGKTYWINPHQIERIEKNPDVTLVLLSGQKVVIKESP
                     EVIIEKIINYRKQIGSFGNED"
     gene            5436..6218
                     /locus_tag="PKW26_05550"
     CDS             5436..6218
                     /locus_tag="PKW26_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HOQ93065.1"
                     /translation="MDLATIIGLIGGSAALVMTVFTSGGSLSGIWDLPSIIFVVGGSF
                     FALFIANPMTTMTKIFGILGRAFKVPSFGEKVLVQNMVGLAEKARREGILALEESLEE
                     LDDEFMKSGLRLVVDGTDGAVIKSILENELNAIENRHLGWINVVTNWAGLAPGYGMMG
                     TVIGLIGMLNNLEDKSSLGPNMAVALITTLYGSMLANWIFTPIATKLSGHNALEVTTK
                     EMIIEGVLSIQAGDNPRILAAKLLTYLDPKSRKLIEADVLKD"
     gene            6222..6938
                     /gene="motB"
                     /locus_tag="PKW26_05555"
     CDS             6222..6938
                     /gene="motB"
                     /locus_tag="PKW26_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOQ93066.1"
                     /translation="MAKDKKEPEEASQEWLVTYGDMITLMLCFFVMLYNPTEVDAIQM
                     AALTASISGDPTGGGQSLASGRLADLGNTINSLPSLEKGKALGLSAKRAVSLFAPDVN
                     TNKITLTSDERGLVITLASDSFFKKGSADLNIEETRDTLLRISQFLSSVELEGRRYRI
                     EGHTDYEVADLEQWPSNWELSVARAVNVLHYLSDFGSDEERFSVAGYADTRPKYSNDT
                     EEARAYNRRVDIIILDEGHF"
     gene            6969..7520
                     /locus_tag="PKW26_05560"
     CDS             6969..7520
                     /locus_tag="PKW26_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOQ93067.1"
                     /translation="MADEDSLDLDGDDGSITGGPKKSNLFSGMLFTILKWVAIVIGGL
                     ILIVTVVVITNKIMSQKNPTTAVYTSSEECKGKRETLSWYSSLGQIRTKSSDILPASI
                     VVEVVLGYKLDDKATASEITSRQIEIKDFLRRYFTEKTSLELRPSNEDKLRVEIRNSI
                     NDDILSTTKIRDVRFLSLEVIEQ"
     gene            7547..8581
                     /gene="fliM"
                     /locus_tag="PKW26_05565"
     CDS             7547..8581
                     /gene="fliM"
                     /locus_tag="PKW26_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOQ93068.1"
                     /translation="MNEVLSQDEIDQLLMAISSGETETDDYKPVSDTRKIKIYDFKRP
                     DKFSKEQIRTVSIMHETFARLTTTSLSAQLRSLVHVHVASVDQLTYEEFIRSIPTPTT
                     LAVINMDPLKGNAVLEIDPSITFSMIDRLFGGTGQGTKVQRDLTDIEQSVMDGIIVRI
                     LANMREAWTQVIDLRPRLGQIETNPQFAQIVPPSEMVVLVTLETKVGDEEGMMNFCIP
                     YITIEPIISKLSSQFWFSSVRRSSTTQYLGVLKEKLSTVDMDVVAEIGSINLPIRDVL
                     GLRTGDIIRLSNTRVGDPLTLSVGNRQKFLCQPGVIGKKMAVQVIGKIKQAESEEFEE
                     LSVEGDESYE"
     gene            8574..>9369
                     /locus_tag="PKW26_05570"
     CDS             8574..>9369
                     /locus_tag="PKW26_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOQ93069.1"
                     /translation="MSDGAISQDEIDALLSGVDMGGLSSAGLSSSKQSSQKLNTETLL
                     EFMTEESEALKATLDAMTGASFSVGAPTVEQVTKEQVLRKIPEMAVGVTADFSEGLSG
                     DHVFVLAPEFAQKIAGLVNKEEDPELSEMSLSVVSEIISQYVGSQITKLSQTGFFPGL
                     ANSPPEAVHVPKAMLRYPSGEFILVSHPVSGEGSVFTLWELIAKDASSQIITALGGNP
                     IQAAPAQSPSMATMGQGMPAMDQMQAMPQMGMGQMAMPQMGGGASFN"
     assembly_gap    9370..9469
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9470..9844
                     /gene="fliN"
                     /locus_tag="PKW26_05575"
     CDS             <9470..9844
                     /gene="fliN"
                     /locus_tag="PKW26_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOQ93070.1"
                     /translation="MGMGQMAMPQMGGGASFNAPNVQSLQYPQLQTGVSSMEQGNIGL
                     IMDVYMEMTVELGRTKKQIKEILGMGEGTIIELDKLAGEPVDILVNHKPIAKGEVVVI
                     DENFGVRVTEILSPIERMSDLR"
     gene            9869..10561
                     /locus_tag="PKW26_05580"
     CDS             9869..10561
                     /locus_tag="PKW26_05580"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOQ93071.1"
                     /translation="MGGGTITKYLKYTQKSFCVLLFFFAFSFLLAQTVENQTEQEIDT
                     QITENALSSYQDETRIVFDNPEEQVLFPEKNTKSGIGSFVRMVVALLVIIGCIYLVFF
                     LIRKGRKNTAVNDAFLKKAASLVLSPGKSIHIITLLDRAYLIGAAENAINLLGEIQDK
                     ELVDTLNLNAEQEAEHKAPDFASLLSLFTPKQRKTENTDSQTFFSNDALNTLKGHKGR
                     LGKKLFDEGENT"
     gene            10558..11397
                     /gene="fliP"
                     /locus_tag="PKW26_05585"
     CDS             10558..11397
                     /gene="fliP"
                     /locus_tag="PKW26_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882163.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOQ93072.1"
                     /translation="MIWLKGYKKAVFFLFLVLVLPATMQVPHLFAQAFPDGATQGTMQ
                     MNPEVEPLSFPLIDLQLRNPTTNQEVAFSVQVLLLLTVLSLAPSILILLTCFLRLSIV
                     FDFIKRALSLQQVPPTTVLNGIAFFLTLFIMWPTFTAIYNNSFKPLSDGEIAIEQAYT
                     EAEAPLRLFMYSQMASNTSYISMFMSMGNLEKPQTLADVPTYVLIPSYILSELTIAFK
                     IGILLYIPFIIIDMVVASILMSMGMIMLPPSQISMPFKLMLFVLVDGWGLLTQQLFNS
                     IIR"
     gene            11406..11675
                     /gene="fliQ"
                     /locus_tag="PKW26_05590"
     CDS             11406..11675
                     /gene="fliQ"
                     /locus_tag="PKW26_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOQ93073.1"
                     /translation="MDLGDVIFLLRDGIFQILMLVSPILGTALVVGLLVAIFQATTSI
                     QEQTLTFVPKFFSILGVLALLGAWMLASLRGYTFKIFEMIPQVAR"
     gene            11675..>12163
                     /locus_tag="PKW26_05595"
     CDS             11675..>12163
                     /locus_tag="PKW26_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOQ93074.1"
                     /translation="MIEDLVANAPSYLLVAARCFALIMTIPLLSTRAVSRVAKVALAG
                     YIAYIAYPLAYAEIWQTEQYQLAMGLFNGAFSLEYLLLLIGEVLIGIIVGLYITAIFA
                     AFSSAGQFFTFQMGFGASEVYDALSQIENPLMGQYLNLIAMIVFLQIKGFQRLFLGGI
                     FRS"
BASE COUNT         3809 a         2264 c         2545 g         3445 t
ORIGIN      
        1 ccaaagtcct ttccgactta gacgccaaaa tcgagctgaa caacaaaatc aaccgggagt
       61 tggaagcaat ggcaaaaacg ctctacgatt attggtttgt gcaattcgac tttcccactc
      121 cgccttcgag tacctcaggc atcgtgggta aaccatacaa atcttcaggt ggcaaaatgg
      181 tgtacaatgc cgagttgaaa agggaaattc ctgaggggtg ggaggttggg actttacttg
      241 atattgctac tttcacaaat ggtttagctt gtcagaaata tagacctata aacaaagaat
      301 ttttgccagt tatcaaaatt cgtgaaatgc gagaaggttt tacaaaagat actgaaaaag
      361 taagaccaga tatttccgaa aatctaattg taaataatgg tgatgtactt ttttcatggt
      421 cagcctccct tgaggttata atatggactg gtggtaaagg agggcttaat caacatatat
      481 ttaaggtaat gtcaaaaaag tatccaagat cgttttatta ttttcaattg atttggtatt
      541 tacaacattt taaaatgatt gctgagttgc gaaaaaccac tatgggacac attacacaag
      601 aacatttaaa acaaagtaga attgttgttc cacctttaga tttgataaaa caacttgaca
      661 aacaattaaa tccaattcaa gaaaaacaag ttttattaca caaacaaaac caacagctct
      721 cttcccttcg agattggctt ttgcctatgt tgatgaatgg acaggtggct tcgagagcct
      781 cagccaccgg ggaatcgaga gcctcagcca ccgaggaata accttatcaa ttccacaaaa
      841 atatatcaag gagaatttac tagatgaaaa acatagagat tttaagcaag gtcaatgaaa
      901 cggatatgcg ggctatctta gaggtttggg aatcttcggt gagggcgacc catagttttt
      961 tgaatagaaa aaaactattt acaaaaagca tttctgccta tatactagaa gcaatattca
     1021 taaaaaggta tgtaaagtgg ccagtggcaa gacaatagca aagacaattc ttttacttct
     1081 tctcattctt atacttattc tcggtgggat gatatggctc gatttccttg gcgttacgca
     1141 aacgaagtcc cgttttgctc ctttgtatcg gatgctcggt cttgagccac aaacgatgag
     1201 ttctatttca agtatacctg acattgccat tgtaaatctt gacaacgatc gtcttgcaaa
     1261 acggcttgaa gctttgacta ttcgtagtga agagctcgat aaacgtgaag aagatatatc
     1321 caaaaaagag caagaaaacc tacaaatcct tgctgaactc gataatctgc gagaatcgca
     1381 agaggagcga gaaaaaacct ttacgagtat gacaaaaacc tttgacgacc gtgaagaaaa
     1441 tattgcaaaa aatgcaaaaa atctttcctc tatgcctcca aaaaatgcgg ttgatattct
     1501 cgttgccatg gaagaccaag acattatcga tgttcttcga aaagcagacc aaatcgcagc
     1561 tgaagagggc acttcttccc ttgtcgcata ttggctttct ttgatgcctg ccgacagggt
     1621 tgcacaaatt cagcgtaaaa tgacgagcaa accgctccaa cttccgtaaa tctctccttt
     1681 ttcttttatt tttctcataa aatcacagat ttctaaaaaa atctcataat aaagcgatgt
     1741 attgatttat ttcctatttt tctatcgagg cataaaaaat aatttaagta atgcccaaaa
     1801 aagttcgatg atagggatgt aggttcttca aaaaagagcc tatcaaagtt aagaaaaggc
     1861 gtacattttt tacatagaca gaggaggttg ccatgttggc atataacctt catcttgaaa
     1921 aaacggcata tatgcccgac ttgtcaaatc aaggattgaa acgacaaact caaggtgatt
     1981 cctcgttttt tgacttgctt caaacagaaa taaaggcgaa gcaaagtgaa gaaacgaaag
     2041 aacccaggga cggacaaaaa acagctacag aatctcaaaa gacgcaaagc aaaacggatg
     2101 aaaatcttgc ggaaaaagat ttgcctgtgg caaaaaacaa tgaatctgaa tcaaaggcta
     2161 ctcaagcgca agcgcaagta aaagaatttg aagaggattc tctttttatt gaaacagatt
     2221 tacaagaaaa ggcgatagcg gattctgaat tcattgactt aaatgcactg gatgaaaaaa
     2281 tttctcccaa gaatgtgaac gaatctctct cattggaaaa tgaatcaaag atgcctcttg
     2341 ttttggaaga cttaaacgag gtgagtgaag agcttgtgag tttaaatgat tcacaacttg
     2401 cctatctttt aaaagaagcg agaagtgagg acgaggtaga tatcagccaa gattctcaca
     2461 tacatgcgtc gctcgaatcg ctttttttcg atgagtcctc ttcttctcta aaggacattt
     2521 caaaagatga tgaggaagcg cttttacaaa gattgcttac gggtgctgtc gatctttctg
     2581 ttttacaaga cgacttttca gatgcagctt tagaagtaga ggattcaaaa actctgggag
     2641 tccaaaacgc ggaaaaaatg ctttttggtt cagaaaatgc cataatctca gttgtcgatg
     2701 aacggacttc ctcaaaagaa gcaaaaaaaa cagatcagtt tgtaaaatct attcagtttg
     2761 aaacagacgg aaaaacagca aatgtagtta tgagcctgcc ttcgcaagca gaagcggtgg
     2821 gttttggttc tgaaaaagaa agtgcaagcc gttttcaaac aatgcttgca aatgaaataa
     2881 aaaactccgc tacagatttg gtgaaaacag gttctattat tcttaaagat aataattctg
     2941 gacttatcaa tttattttta aagccagaag cgcttggaga tgtaaagata cgtttaaata
     3001 tttccgacac tttaataagt gcgaaaatta ttgtcgacag tcaagaagca ttgaatgctt
     3061 ttaaagactc gataggcgct ttaaaacaag cttttgagca gggtggcttt gatacaggca
     3121 gttttgatct ttcatggtcg ggacaaggcg gttcagggca agaaaaaaac gagcacaatg
     3181 caaaagcaca tttttatagc gatgcgctac ctgagcttgt gcaagatgaa cttgaatcaa
     3241 atgaacgaat tttggcattg atgaaaaatg caatacaata cggtacttct atcgtaaatg
     3301 taattgccta aaaaagagga gaaaccatgg attttacggc aacaatgagt gcaacagatc
     3361 agctgtatgc aaaaaataca gttgatgctt ttaacaaaac acttacagta aacggacgtc
     3421 agcaaagtaa ccagttgggc aaggatgatt ttttaaagct tttgttggca caactcgcca
     3481 atcaagatcc aaccagtcct atggaaaata ctgagtttat tgcacaaatg gcacagtttt
     3541 cttccttgga acaaatgaca aatatgagta ccgaatttag tcgtttggca aatatgcttc
     3601 actccaatga ggcaatcaat ataatcggaa aacgagttga tatcgaagaa ggtgctcttt
     3661 cgctcagcgg tcacgttgac ggcgctgttt taggggtaaa tccacaagta gaaataaatg
     3721 gcatgctata cagcatggaa gcaattaaac gcgtatattc agaataaaag agggcaaaaa
     3781 ctatgatgcg atcactttac gccggtgttt ccggtatgca aaaccaccaa acacgacttg
     3841 atgttattgg taacaacgtt tcaaacgtaa acactaccgg atttaaacgt ggtagggtaa
     3901 attttcaaga tatgatttct caacaacttt ctggtgcggc aaagccaaca actgaagttg
     3961 gtggggtaaa cccaaaagaa gttggacttg gcatgatgat tgcttccatc gatactatct
     4021 ttacgcaggg aaacctacag tcaaccggtg ttgcaacaga tgtggctatc caaggcaatg
     4081 gtttttttgt tttgaaaaac ggcgagcagt ctttttactc aagggctgga gcctttggct
     4141 tggataacga gggaacgctt gtaaatcctg caaatggttt tagggtacaa ggttggatgg
     4201 cccaagaaat ggatggagaa atggtgctca atacagcaag taatgcacag gatctcatta
     4261 tccctgttgg aacaaaggac cctgcaaagg caacagaaaa cgtaaacttt gcgtgtaact
     4321 taaataaaaa cacgccagaa atcttagaag gtgcttctcc tgcagatatt gcaaagggca
     4381 catgggcaac agaatttaaa gtctatgata gttttggaaa tgaccacaat cttaccgttt
     4441 cttttacacg agttgtagga aacccaaatc aatgggaagg aagagtaatc gtcgatccag
     4501 aaaatgctga agaaacacaa acccgtgttg gtcttggaac aacagacggc gtggaagatt
     4561 ttttccttgt aaactttgat aacacaggaa cccttctttc tgtaacagat tccgcaggaa
     4621 acaatacaaa ccccgaaggt gaaatattgc ttcaaacctc ctttacggta atggattcaa
     4681 accccgatgc tgatggaaac ccaacccgac aaacgctcaa tattaacttg ggtacaatcg
     4741 gcagtcaaaa aaatactatt acgcaaagtg catcacaaag ttcaacgaag gctttttatc
     4801 aagacggtta taccatgggc tatcttgaca actttaagat tgactccagc ggaattatta
     4861 ctggtgtcta ctcaaatggc acaaaccgaa caattgggca aatcgcactt gcaaccttta
     4921 caaaccaagg cggcttagaa aaagttggag acaataactt tgtcgagtca aataactcag
     4981 gtttagcaaa tattggtacc tcgggtatag cagggaaggg tagtctttta gcaggtgctc
     5041 ttgaaatgtc aaacgtagat cttaccgacc aaatgaccga catgattgta actcaaagag
     5101 gatttcaggc gaattctaag acaattcaaa ctgcagatac gcttttagag acagtaatga
     5161 gcttaaaacg ataataattt cgtaaaacta cttgacgtat aagggataag catagaaaat
     5221 agaaagatga tagaagtaac acgattagat ggcaaaacat attggataaa tccgcatcaa
     5281 atagaacgca tagaaaaaaa tccagatgta accctcgtac ttctatctgg gcaaaaagta
     5341 gttataaaag aaagccctga agtcattata gaaaaaataa ttaactatag aaaacagata
     5401 ggttcctttg gtaacgagga ttagggagga tattcatgga tttggcgaca attattggtt
     5461 taattggtgg atcagctgct cttgttatga ccgtttttac atcaggtggt tcgctttcag
     5521 gtatttggga ccttccttct attatcttcg ttgtcggtgg ctcttttttc gctctcttca
     5581 tagcaaatcc tatgacaacg atgaccaaaa ttttcggtat tttaggacgt gcttttaaag
     5641 tgcccagctt cggtgaaaaa gtccttgtcc aaaacatggt cggcttagct gaaaaagccc
     5701 gtcgtgaggg gattcttgcc ctcgaagaaa gtttagaaga gcttgatgat gagtttatga
     5761 aaagcggact tcgcctcgtt gtcgacggta ccgacggtgc tgttataaaa agtattttgg
     5821 aaaatgaact gaatgcaatc gaaaaccgcc acttgggatg gattaacgtt gttactaact
     5881 gggcagggct tgcaccaggc tatggtatga tgggtaccgt tatcgggctt atcgggatgt
     5941 taaacaacct cgaagataaa tcttctcttg gtcctaacat ggctgttgcc cttattacca
     6001 cgctttacgg ctctatgctt gcaaactgga tttttacacc tatagcgaca aagttgtcag
     6061 ggcacaacgc ccttgaggta acaacaaaag aaatgataat tgaaggagtg ctttctattc
     6121 aggcagggga taaccctcgt attttagctg caaaactctt aacctatctt gatccaaaat
     6181 caagaaagct tatagaggca gatgtattaa aggattagta aatggcaaaa gacaaaaaag
     6241 aaccagaaga agcgtcccag gagtggctcg ttacctatgg cgatatgatt acactcatgc
     6301 tctgtttttt cgttatgttg tataatccaa cagaggtgga tgctatacaa atggcagcct
     6361 taactgcttc tatcagtgga gatccgacag gtggaggaca atcgcttgct tctggacggc
     6421 ttgccgactt gggaaacaca attaacagcc tgccatcttt agaaaaaggc aaggctttgg
     6481 gccttagtgc aaaacgtgcg gtaagcttat ttgcaccgga tgttaacaca aataaaatta
     6541 cgctcacaag cgatgagaga ggcttggtta taacccttgc ctctgattcc ttttttaaaa
     6601 aaggaagtgc ggatctaaat atagaagaaa caagagatac cttgctgcgt atttctcagt
     6661 ttttatcttc tgttgagcta gagggcagga gatacagaat cgagggccat actgattacg
     6721 aagttgcgga tttggaacaa tggccgagta attgggagct ttctgttgca cgggcggtaa
     6781 atgtgcttca ttatctatca gattttggtt ctgatgagga gcgtttttct gttgcaggct
     6841 atgctgacac tcgccccaag tactcaaacg atacagaaga agctcgtgca tataatagaa
     6901 gagtagacat aattattctg gacgaagggc atttttaatg ttaaaataga atataaggat
     6961 ggtagaaaat ggctgatgaa gatagcttag accttgatgg cgacgatgga agtattactg
     7021 gtggtccaaa aaaatcaaat cttttctctg gtatgttatt taccatactt aaatgggttg
     7081 caattgttat aggaggctta atactcattg ttaccgttgt tgttataaca aataaaataa
     7141 tgagtcaaaa aaatccgacg acagctgtgt atacatcttc tgaagaatgc aaggggaaaa
     7201 gagagacgct ttcgtggtat agttccttgg gacaaatccg cacaaaatcg agtgatatct
     7261 tacctgcgag tattgttgta gaagttgttt taggctataa gcttgacgat aaagcaactg
     7321 cttcagaaat aacttcaagg caaattgaaa taaaagattt tttacgacgt tattttactg
     7381 aaaaaacaag ccttgaattg cgaccttcta acgaagacaa actacgtgta gaaattcgca
     7441 actcaataaa tgatgatatc ttgagtacaa caaagatacg agatgttcga tttttatcgc
     7501 tcgaagttat agagcaataa aaaaaatata aatagagggg aagtgcatga acgaagttct
     7561 gtcacaagac gagattgatc aactgctcat ggctataagt tctggtgaaa cagaaacaga
     7621 cgattataag ccggttagtg atactcgaaa gattaaaatc tacgacttta aaagaccaga
     7681 taaattctct aaagagcaga ttcgaactgt ttctattatg catgaaacct ttgctcgtct
     7741 tactacgaca agcctttctg cccagcttcg aagtctagtg catgtgcatg tcgcttccgt
     7801 tgaccagctt acctatgagg aatttattcg ttctattcca acaccaacaa cacttgctgt
     7861 tatcaacatg gaccctctta aaggaaatgc tgttttggaa atagaccctt ctattacctt
     7921 ttccatgatc gaccgccttt ttggtggaac aggacaggga acaaaggtgc aacgtgacct
     7981 taccgacatc gaacagtccg ttatggacgg tattatagtg cgaattttgg caaatatgcg
     8041 cgaagcttgg acacaggtta tcgatctacg tcctcgtctt gggcaaatag aaacaaaccc
     8101 acagtttgca caaattgttc caccttcaga aatggtggtt ttagttacct tggaaacaaa
     8161 ggttggcgat gaagagggca tgatgaactt ttgtatcccc tatattacga tcgagcctat
     8221 tatttccaag ctttcatcac agttttggtt ttcttccgtg cgaagaagtt ccacaacaca
     8281 gtacctcggc gttttaaaag aaaagctctc cacggtagat atggacgttg ttgccgaaat
     8341 tggttcaata aatttgccta ttcgagacgt tttaggttta agaactggcg atattatccg
     8401 attatctaat acgagggttg gtgatccatt aacgctcagt gtcggaaata gacagaaatt
     8461 cttatgtcag cctggtgtaa ttggtaaaaa aatggcagta caagtcattg gaaaaatcaa
     8521 acaagctgaa tcagaagaat ttgaagaatt atcagtagaa ggagatgaat catatgagtg
     8581 atggtgccat ttctcaagac gaaatagatg ctttattatc tggtgtagat atgggaggac
     8641 tgagctccgc tggtctttca tcttctaagc aaagtagtca aaaattaaat accgaaacac
     8701 ttctcgaatt tatgacagaa gaatctgagg cgcttaaagc gaccttagat gcgatgactg
     8761 gggcaagttt tagtgtcggc gcgccaactg tagagcaggt gactaaagaa caggttttgc
     8821 gaaagattcc cgaaatggcg gttggagtta ccgctgattt ctcagaaggt ttgtcagggg
     8881 atcacgtttt tgtgttagca ccggagtttg cacaaaaaat cgcaggatta gtcaataaag
     8941 aagaagatcc agagctcagt gaaatgtctc tctccgttgt atcggagatt atttcgcagt
     9001 atgtgggttc acaaataacg aaattaagcc aaactggttt tttccccggt cttgcaaata
     9061 gccctccaga agcagttcat gttcccaagg caatgttgcg ttatccttcg ggtgaattta
     9121 ttctcgtatc tcatcctgtt tccggtgaag gctcagtgtt tacactttgg gaattgatcg
     9181 caaaggatgc atcttctcaa attatcacag ctcttggtgg aaaccccatt caagcggctc
     9241 ctgcacaaag tccttcaatg gctacaatgg ggcagggaat gcctgctatg gatcaaatgc
     9301 aagcgatgcc tcagatgggt atgggacaaa tggcgatgcc tcaaatgggc ggtggagcaa
     9361 gcttcaatgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tgggtatggg
     9481 acaaatggcg atgcctcaaa tgggcggtgg agcaagcttc aatgcaccaa atgtgcagtc
     9541 cttacaatat ccccaattac aaacgggtgt ttcgagcatg gaacaaggaa atatcggtct
     9601 tatcatggat gtatacatgg agatgaccgt tgagctcggg cgaacaaaaa aacaaattaa
     9661 agaaattctt ggaatgggtg aaggtaccat cattgagctt gataagctcg ctggtgagcc
     9721 tgttgacatt cttgtgaatc ataaaccaat agccaagggt gaggttgttg ttatagatga
     9781 aaactttgga gtgcgtgtaa ccgaaatcct ctctccaata gaaaggatga gcgatttgcg
     9841 ctaaaaaaaa tagtgatgga gtgatctcat gggtggggga actattacga aatacttaaa
     9901 atatactcaa aaaagttttt gtgtgctgct ttttttcttt gccttttctt tcttactggc
     9961 tcaaacagtg gaaaatcaaa cagagcaaga aattgatacg caaataacag aaaatgcgct
    10021 ttcgtcatat caagatgaaa ctcgcatagt ttttgacaat cctgaagagc aggttttatt
    10081 tccagaaaag aatacaaaat ctggaatagg ttcttttgtt cgcatggttg tggcgctttt
    10141 agttattatt ggctgtatct atctcgtttt ctttttgata agaaagggaa gaaaaaatac
    10201 cgctgtcaat gatgcctttt tgaaaaaagc agcgagcctt gtgctttctc caggaaagtc
    10261 aatacatatt ataacgcttt tagatagggc ttatttaata ggggctgctg aaaacgcaat
    10321 aaatcttctc ggtgaaatcc aagacaaaga gcttgtcgat acattaaatc tcaatgcaga
    10381 gcaagaagca gaacataaag caccagattt tgcaagtttg ctttcgcttt ttactccaaa
    10441 gcaaagaaag acagaaaaca ccgactcgca aaccttcttt tcaaacgatg ctttgaatac
    10501 ccttaaggga cataaaggtc gcttaggaaa aaaattgttt gacgaaggag aaaatacatg
    10561 atatggctca aaggctataa aaaagctgtc ttttttcttt ttctcgtgct tgttttaccc
    10621 gctacaatgc aggtgccgca tctctttgca caagcctttc ccgatggtgc aacgcagggt
    10681 acaatgcaga tgaatccaga agtagagcct ttgagctttc ccctcataga tttacaactg
    10741 cgaaatccca cgacaaacca agaagttgct ttttctgtgc aagtactgct tcttttaacg
    10801 gttttatctc ttgcaccaag tattctcatc ctactaacct gttttttgcg actttctatt
    10861 gtatttgatt ttatcaagcg tgcgctttct ttacagcaag ttcctcccac aacggtttta
    10921 aatggaatag cttttttctt aaccttattt attatgtggc ctacatttac cgctatttat
    10981 aacaattctt ttaagccact ttctgatgga gaaatcgcta tagaacaagc ctatacagaa
    11041 gcggaggcac ccttacgtct ttttatgtac agtcaaatgg cttcgaatac aagttatatt
    11101 tccatgttta tgtccatggg aaatctcgaa aagccacaaa ctcttgcaga tgtgccaacc
    11161 tatgtgttga ttccctcgta tattttaagt gagctgacaa tcgcttttaa aataggtatt
    11221 ttgctgtata ttccctttat tattatagat atggttgttg catccattct tatgtcgatg
    11281 ggtatgataa tgctgccgcc ttcgcaaatc tcgatgccct ttaaactcat gctttttgtc
    11341 cttgttgacg gctggggctt gttgacgcaa caattattta attcaattat acgataggag
    11401 aataaatgga tttaggagat gtcatttttt tattgcgcga tggaattttt caaattctta
    11461 tgcttgtttc gccgattttg ggaacagcct tggttgttgg gcttttggtt gcaatttttc
    11521 aagcaacaac ctccatacaa gaacaaacgc ttacctttgt tcccaagttt ttttctattc
    11581 taggcgtttt agctctcctt ggagcttgga tgcttgcctc cttgcgaggt tataccttta
    11641 aaatatttga aatgataccg caggtcgctc gctaatgata gaagatcttg ttgcaaacgc
    11701 tccttcatat ctcctcgttg cagcacgatg ctttgcgctc attatgacga ttcccctgct
    11761 ttctacccga gctgtctccc gcgtcgcaaa agtggccctt gctggctaca tagcctacat
    11821 tgcctaccca ctcgcatatg ctgaaatatg gcaaactgaa caataccagc ttgctatggg
    11881 tttatttaac ggtgcctttt ctctcgagta tcttctttta ctcatcggtg aagtacttat
    11941 aggtattatc gttgggcttt atattacggc aatttttgcc gcctttagct ctgcaggaca
    12001 attttttacc tttcaaatgg gctttggagc ctcagaagtc tatgatgcgc tttcacaaat
    12061 cgaaaacccc ctcatgggtc aatacttaaa cctcatcgct atgattgttt ttttgcaaat
    12121 aaagggcttt caaaggcttt ttctcggagg tatttttcgt agc
//
LOCUS       DAOSSW010000053        11806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_5405_length_11806_cov_6.773892, whole genome shotgun sequence.
ACCESSION   DAOSSW010000053 DAOSSW010000000
VERSION     DAOSSW010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 11806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11806
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5405_length_11806_cov_6.773892"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PKW26_05600"
     CDS             complement(<1..476)
                     /locus_tag="PKW26_05600"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOQ93075.1"
                     /translation="MKQMIKLGLILALYTSIACVTLAFVNDLTKDTIASAKDKELNEG
                     LKIVFAQADSFEQLDSSLFPSINDVTIDAVYLAKANDAIIGSVIKASGATYNDATILV
                     GITTESTMTSIEFLELTDTPGYGQQAKEPAFKDQFIDKAIDETLALGTNVDAISGST"
     gene            complement(473..1474)
                     /locus_tag="PKW26_05605"
     CDS             complement(473..1474)
                     /locus_tag="PKW26_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOQ93076.1"
                     /translation="MAKVKTSNELYISPSPHITGPETSQKIMFLVILSLIPLCIYGVY
                     LFGLPALLTIGVAVVSCLVFEALFQVLTKQSILIKDGSAIVSGIMLALVLPPQLPLWM
                     TVLGAAFAMIVAKAFFGGIGANVFNPALTGRAFLFVSFPAAMGTWPLPRTAQSLVETV
                     TSATGGIIDEVTSATALSSSSLIVDSKTYFDFFIGNRAGCIGESSIALILLAFAFLAV
                     TKVIDWRAPLAMVATVALASFLAGGDVLMAMLSGGLLFGAVFMATDYATSPVTKKGRL
                     VFGFGAGLITFLIRQFGGYPEGVMFSILIMNALTPFLNKLIPQKYGYIKPSKKVAKK"
     gene            complement(1474..2871)
                     /gene="rsxC"
                     /locus_tag="PKW26_05610"
     CDS             complement(1474..2871)
                     /gene="rsxC"
                     /locus_tag="PKW26_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOQ93077.1"
                     /translation="MKMHTFKGGVHPPEKKGFTADKAIESVYPSTNMVSIPLTMGGKP
                     NELLVSVGDTVTRGQKIADNTDCLSAPVHSSISGKVKKIETKLLADGSTGQCITIEGD
                     GQNIESFMPKLDPFTCSKTEALERVREAGIVGMGGAAFPTHVKLFPPKEKPISVVIAN
                     GAECEPYLTIDDMTMQERATNIIDGLAIATKIVGAPKALLAIEDNKKHLLPILNEAIE
                     KSGNTVISLVLTKTKYPQGGEKSLIKAVLKKEVPSCGIPADIGCVVLNVNTLCAISDA
                     FRLGKPLIDRPLTISGAACENPKNIVVPIGTVLADLIPETISIKEDLVKILSGGPMMG
                     VSLPNTSFPIAKNTSGILFLNKKETDLTTESQCISCGRCIDVCSCRITPVMIVKELAA
                     GNMAEAIRYGLMDCVECGSCVYVCPAHVQLIQRIRTGKQIRRSEIAQELQIQEKNLAD
                     CGKVMEADCGCGGDK"
     gene            3027..4079
                     /locus_tag="PKW26_05615"
     CDS             3027..4079
                     /locus_tag="PKW26_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93078.1"
                     /translation="MKKSILFFLVFSISTVLVSAYNPPPSGELLHQFSSSELLSGGSL
                     STGGAFKNVRPESIGINPSLIAGEQRWVVDLGYTAFIGTNDLEGFGSAFLAGFIIPSR
                     YGNLALTMDGIFTNNLVKPHLGNSFLVDAAFAKDLTDDLYLGIGMKGGFGPEGDWALA
                     GDVGFWYRIKSLRFLPFLKDLRWAFSLTNMGKSFLASEVGIIEGKEVSASPSLFTPTI
                     GLAGTLFSTKHIAGGLGIDLSFPIFQNLIADIGFELLVANAFRIKTAWDMNLREVIAG
                     HGPSLPSLSLSYTFNFSTKELDFMEKHGWQENEMLVSTAYKNLYKDVHLASGGLLLFL
                     GQKDFEAPVISLWGEE"
     gene            4079..7195
                     /locus_tag="PKW26_05620"
     CDS             4079..7195
                     /locus_tag="PKW26_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOQ93079.1"
                     /translation="MKINRIVIIFLLILLFSPLFSQAKTVYISPNNDGIQDELAIPLS
                     IKDKRYISEWNLIVLDSENTIVRTIGNKHAKETNATSVFQSLFAEKGFKPFWTNLKKL
                     FAPKKGIEVPEVVVWNGFLDSGEVAPDGNYSYYFIAKDDNGNEAKTSMYSVVVDNTPP
                     AIALLQPKDEAKIFGSNLRNAIKIEQDGSSEDLWEADIKDASGNSVKSYSWKNDSPKT
                     IEWDARNNAGDLVADGVYSYAITATDRAGNKSEKAEVLNIIYDALPRSVNLTVLGSPF
                     SPNADGIQDSLRFEPSIPNASGLLSWTIDIISPNQESRIYKRFEGKTTPQAFSFDGKS
                     EAGSVLGDGEYQALFQANFNNGQIAKMSRNFIIDTRAPQASLVLETPIFSPDGDGNLD
                     LAIIAQSVSKEKNWQAEILDANRKKVRSYSFGDNPPEKVQWDGLHDNGSLAEDGLYSY
                     KLIATDDAGNTGFAETKLFELNTGTTEVILTVQNPAFSPNKDGVNDVLVLTPVVKTKS
                     EIVEYRLDIFNERGESVKSFADKKALPRNFQWDGLDDNGSLCPDALYTAQLETVSKNG
                     SEAKTLSQPITLDTQYPTLELSADYLVFSPNDDGKKDILPIKIAGSEENQWIAEIKNQ
                     AKTTVKKITWQGSPASFDWDGKDENNNTVADGNYSLLVSSTDDAGNKTEKQIPLIVVD
                     NRPTKAFVTAELSAFSPNADGIKDEQVFTIMTSLQEGIASWSLAILSENGASVQKWTG
                     NDAKSLPAKINWDGKDSSGKVVEGSLTADLEIIYEKGDEVFAKTSSFLSSITPPQLLV
                     KTSPQYFSPDNDGQDDELFINLSAKSAVPFTSWSFEINDPQNGKTFWKTSGKSAITER
                     ITWDGKSNTGELVQSATDYPYTFTVSDDVGMTSRFEGLISVDVLVIRVGDVLKIQIPS
                     IIFRPDNADFKSKKEVANGLEQSVIDNNVRVLKRIAEILNKFRDYTVRVEGHANNVTN
                     TEKEEIEELVPLSQARAAFVKEELRKYGVSSARLSTVGLGGRQPVVPHSDRDNWWKNR
                     RVEFILNK"
     gene            complement(7311..7769)
                     /locus_tag="PKW26_05625"
     CDS             complement(7311..7769)
                     /locus_tag="PKW26_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOQ93080.1"
                     /translation="MKHDISSISFLISIVHAHTASFLKKRLSDEGLEKLATSHGHILF
                     QLAVYEELSMSEVARLVYKDKSTTTALCKKLEEIGLIERKQSKQDKRQTKIRLSEKGR
                     QYEDSLTKISQELTETAFLGFSEAEKKEMIEKLDAIKANFQNLEGEPKNS"
     gene            7850..8632
                     /locus_tag="PKW26_05630"
     CDS             7850..8632
                     /locus_tag="PKW26_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93081.1"
                     /translation="MIGFYIKKNFFDGWDNFLSLIVFNLISYALLIGLFFLIGFFAET
                     PVLFFSFLFIGIYLVTVFYFSMSILTAEIAHNKSVSILSLFKNIVFVWKDALFLSLLF
                     LALFISATIALPFYYNLGGIFGLAMSGIIFWLILMTLLALQWFVPLSVEFKGGFFKTL
                     KKSYLLLMDNGGFTFFLAIYSIVLFLLSALLAFFAPGFSGISLAQNNALKLRLYKYDW
                     LEENPDIAQTKERKNIPWDELLAEDDEIIGPRSFKSLIFPWK"
     gene            8649..9686
                     /gene="asnA"
                     /locus_tag="PKW26_05635"
     CDS             8649..9686
                     /gene="asnA"
                     /locus_tag="PKW26_05635"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOQ93082.1"
                     /translation="MSRTIVPKNYTSLLSVKETEYAIVLIKDFFQLALSTELNLTRVT
                     APMFVEQGKGLNDDLTGVERAVNFPVKDMDDRRLEIVHSLAKWKRVKLSDLGLEQGFG
                     IYTDMNAIRADEELDNIHSLYVDQWDWELVIGEENRSVEYLKEVVKKIYRCMKRTEFF
                     LYDRYPQLEIRLPEKITFIHAQELLDRYPDLSPKERENAVAKEFGAVFIIGIGANLSN
                     GMPHDGRAPDYDDWITKTADGRQGLNGDLLLWNPLLDSALELSSMGIRVSPESLKSQL
                     IERNCTQRESLFFHKKLLNGELPLCIGGGIGQSRLCMFFLQKAHIGETQVSTWPEEMI
                     EICKKSGIQLL"
     gene            9683..10630
                     /locus_tag="PKW26_05640"
     CDS             9683..10630
                     /locus_tag="PKW26_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93083.1"
                     /translation="MTEQKFTARELEEGLGTFFTRGFSHIRVEDSSLTENKQALLAFL
                     RSIAKKEGQVLFEFFLSVEMLEKDIVNALAETASTLVISFNGGEQKNFAKKIALLNDL
                     GLSFGFIVELNEKNTETKKLFSRLLEEIAGYFPNHVYFSFEKSFASKLTEKDAELLRA
                     ISHCFELFYTEGRAVPWFKSLLLSLKISAYAFISDFYEWFLLNNYTLPTETEEKYPFA
                     KILKMQERFIQFKLEEKKISYIYPVIEDILRLHAAFSEAIVEGKETELVLHYSPEDTL
                     SPSSFYFLRFYDEVCAEKTAIRVFLTEEGPEYEILPFFT"
     gene            10692..11348
                     /gene="grpE"
                     /locus_tag="PKW26_05645"
     CDS             10692..11348
                     /gene="grpE"
                     /locus_tag="PKW26_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOQ93084.1"
                     /translation="MKEAEKKDKKAVEQDEAETLKKDDDLQNELNEEEIREKEVSETC
                     EKEEKTLEEAFECLKKENADLKDQYLRKLAEFDNYRKRMIKEKQEAIDYANTNLLLDL
                     LTTLDDFDRAIQAASQNSGEEKKGDKKQAEASILEGLCLMNKQMLSMLENKYNLLPFG
                     KAGEAYDHDRHEAIGSMQMPVAEPVLAEVYLKGYLLKERVIRPAKVMVHMPDGTETEK
                     "
     gene            11436..>11806
                     /locus_tag="PKW26_05650"
     CDS             11436..>11806
                     /locus_tag="PKW26_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOQ93085.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGGEPIVIQNSEGGRTTPSIVGFTA
                     KGERVVGQPAKNQMITNPENTVYSIKRFIGHRYNELSGDAKMVPFKVEPYGNDDVRIN
                     VGGKQYSTQEISAFTLQKMKKT"
BASE COUNT         3548 a         2141 c         2500 g         3617 t
ORIGIN      
        1 gttgaaccag aaatagcatc gacgtttgta cctaaggcaa gcgtttcatc aattgcttta
       61 tcaataaatt ggtctttaaa tgcaggttcc tttgcttgtt gaccgtatcc aggtgtatca
      121 gttaattcta aaaactctat gctcgtcata gtgctttctg tggtaattcc cacaagaatt
      181 gttgcatcat tatatgttgc accacttgct tttataactg acccaataat tgcatcgttc
      241 gcttttgcaa gataaaccgc atcgatagta acatcgttta ttgatggaaa aagactagaa
      301 tcgagttgtt cgaaggaatc ggcttgtgcg aagacaattt tgagcccctc gtttaattct
      361 ttatcttttg cacttgctat cgtatccttt gttaggtcgt ttacaaaagc aagggttacg
      421 caagcaatgg aggtataaag ggccaatatg agccctagtt taatcatttg tttcattttt
      481 tagccacctt ttttgaaggt ttaatgtatc cgtatttttg gggaatgagt ttgtttaaga
      541 agggagtgag agcgttcatg atgaggatac taaacataac tccttcggga tagccaccaa
      601 attgacggat taaaaaggtt ataagaccag caccaaatcc aaagacgagt cttccttttt
      661 ttgttactgg acttgttgca tagtctgttg ccataaaaac agcaccgaac aagagcccgc
      721 cagagagcat cgccataaga acgtcgccac ctgccaagaa gcttgcaaga gctactgttg
      781 ctaccatagc gaggggagct cgccagtcga taaccttggt aacagcgagg aaggcaaagg
      841 caagcagtat gagtgcaatg cttgactcac ctatacaacc agctctatta ccgataaaaa
      901 agtcaaaata tgtttttgaa tcgacaatga gtgagctcga tgaaagggcg gttgcactgg
      961 taacttcatc aattatacca cctgttgcgc ttgttactgt ttctacgagc gattgcgctg
     1021 ttcgtggtag cggccatgta cccatagctg cggggaaact gacaaagaga aaggctcttc
     1081 ctgttaatgc tgggttaaat acgtttgcac ctatgccacc aaaaaaagct tttgcaacaa
     1141 tcatcgcaaa ggctgctcca agaacggtca tccaaagcgg gagttgcggt ggtaaaacaa
     1201 gggcaagcat aatacctgac acaattgctg agccgtcttt tattaagata ctttgctttg
     1261 taaggacttg aaaaagcgct tcgaaaacaa ggcaggagac aactgctacg ccgatagtaa
     1321 gtagggcagg taaaccaaag aggtagacgc cgtagataca aaggggaatt aagctcagta
     1381 ttaccaaaaa cattattttt tgacttgtct caggtcctgt gatatgaggg cttggactta
     1441 tatagagttc atttgaggtc tttacttttg ccattactta tctcctccac aaccgcagtc
     1501 tgcttccata acttttccgc agtctgcaag atttttttct tgaatttgaa gttcttgagc
     1561 gatttcactt cgtcttattt gctttccggt acgtattctt tggatgagtt gcacatgagc
     1621 agggcaaacg taaacgcagg aaccgcattc aacacagtcc atgagtccat aacgaattgc
     1681 ttcagccata tttcccgcag cgagttcttt tacaatcatc acgggggtaa ttctgcatga
     1741 acagacatct atacagcgtc cgcagctaat gcactggctt tctgttgtga ggtcagtttc
     1801 ttttttattc aaaaagagga ttcctgatgt gttttttgca atggggaagg aggtgtttgg
     1861 aagggaaact cccatcatgg gaccgccaga gagtattttt actaaatctt cttttatgct
     1921 aattgtttca ggaattaaat ctgcaaggac agtaccaata ggaactacaa tgttttttgg
     1981 gttttcgcat gcagcacccg agatagtcaa aggtctgtcg atgagcggtt ttcctagcct
     2041 aaatgcatcg gatatagcac aaagcgtatt tacgtttaaa acgacacaac cgatatctgc
     2101 tggtatacca caactcggca cctctttttt gagaacagct tttattaggc ttttttctcc
     2161 gccttgagga tattttgttt ttgtgagtac aagagagatt actgtattcc ctgacttttc
     2221 gatagcttca ttgagtatag gtagaagatg ttttttattg tcttctatag cgagtaaagc
     2281 ttttggtgcg ccaactatct ttgttgcaat ggctaagcca tctataatgt ttgttgccct
     2341 ttcttgcata gtcatgtcgt cgattgtgag gtagggctcg cattcagctc cgtttgcaat
     2401 gacaacagat ataggctttt cttttggggg gaaaagtttt acgtgagttg gaaaagccgc
     2461 cccacccata cctacaatac ctgcttctcg tacacgttct agagcttctg ttttggaaca
     2521 ggtaaaaggg tcgagcttag gcataaaact ttctatgttt tggccatcac cttcgatagt
     2581 gatgcattga ccagtagagc cgtcggcaag gagttttgtt tctatttttt ttaccttacc
     2641 ggagatagat gaatggacag gagcagaaag acaatcggta ttatctgcta ttttttgtcc
     2701 gcgagtgact gtatcgccga cagaaactaa aagttcgtta ggtttgcctc ccattgtaag
     2761 gggaatgctt accatatttg ttgaaggata gacagactct atagccttat ctgctgtaaa
     2821 tcccttcttc tctggcggat gaacaccgcc tttaaaagtg tgcattttca tagggttatg
     2881 atatgagtta gcaattatta agtcaataga aacgagcctt aattatagat aaaattttat
     2941 tgacaaaagg ttttattttt ttgtgctaga ttttaattta cttcattggt aaaataaaac
     3001 tatgtacaat tcgggaggtt ttttacatga aaaaaagtat actttttttt ctcgtattta
     3061 gtatcagcac tgtgttagtc agtgcatata atccaccacc tagtggtgag ttgcttcatc
     3121 aattctcatc atcagagctt ttatcaggtg gttctctttc aacaggcggc gccttcaaaa
     3181 atgttcgacc agagagtatc gggataaacc cctcactcat tgcgggcgaa caacgttggg
     3241 tcgttgacct tggctatact gcatttattg gtacaaatga cctagaagga ttcggttctg
     3301 cctttcttgc gggctttatt atcccttctc gttacggaaa tcttgccctt acaatggatg
     3361 gcatttttac aaataatctt gtaaaaccac atttaggtaa ttcctttttg gttgatgcag
     3421 cttttgcaaa agatttaacc gatgatttgt atcttggtat tggcatgaag ggaggttttg
     3481 gtccagaagg cgattgggct cttgcgggag atgttggctt ttggtatcgc ataaaaagcc
     3541 ttcgtttttt acccttttta aaagaccttc gatgggcttt ttctctaacc aatatgggaa
     3601 aatcttttct tgcaagtgaa gttggcataa tagaaggcaa ggaagtaagc gcatcaccga
     3661 gcctttttac accgacgata ggacttgcag gtaccttatt ttcgacaaaa catattgcag
     3721 gcggtttggg gatagattta tcttttccca tctttcaaaa cctcatagca gatattggct
     3781 ttgaacttct tgttgcaaat gcctttcgta taaaaacagc atgggatatg aatctacgag
     3841 aagtgatagc tggtcatgga ccttcattac caagtctttc attgagctat acgtttaatt
     3901 tttctacaaa agagcttgat ttcatggaaa aacatggctg gcaagaaaat gaaatgcttg
     3961 ttagcacagc gtataaaaat ctctataaag atgtgcacct cgcttcaggg ggacttttat
     4021 tgtttttagg acaaaaagac tttgaagcac ctgtaatatc actttggggg gaagaataat
     4081 gaaaataaac cgtattgtaa ttatatttct tttaatactt cttttttcgc cgttattttc
     4141 gcaagctaaa acagtctaca tatcgccaaa taatgacggt atacaagatg aacttgcgat
     4201 tcccttatcg attaaggata agcgatatat tagcgagtgg aatctcattg ttttagatag
     4261 tgaaaacact atagtgcgaa cgattggaaa taaacacgca aaagaaacaa atgcaacaag
     4321 tgtctttcaa tccttgtttg cagaaaaggg ttttaagcct ttttggacaa atttaaaaaa
     4381 acttttcgct cccaaaaaag gaattgaggt tcctgaggtt gttgtttgga atggttttct
     4441 cgatagtgga gaagttgcac cagatggaaa ctattcctat tattttatag caaaggatga
     4501 caatggaaat gaggcaaaga cgagcatgta tagcgtcgtt gttgacaata cgcctcctgc
     4561 tattgcgctt ctgcaaccaa aagacgaggc aaaaatattt ggctcaaatc ttcgaaacgc
     4621 cattaagata gaacaagatg gttccagtga agatttatgg gaagcagata taaaagatgc
     4681 ttcgggaaat agcgtaaaaa gctattcttg gaaaaatgat agtccgaaaa caatcgaatg
     4741 ggatgcgcgc aacaatgcag gcgaccttgt tgctgatggt gtgtatagct atgcaattac
     4801 tgctactgat cgcgcaggca ataagagcga aaaagcagaa gtcttaaata ttatttatga
     4861 tgctcttcca cgttctgtaa atcttactgt tcttggtagc cccttttccc caaatgcgga
     4921 tggtattcaa gatagccttc gtttcgagcc gagcattcca aatgcaagcg gtttactctc
     4981 ttggactatc gatattattt ctccaaatca agaaagcaga atctataaac gttttgaagg
     5041 aaaaacgacg ccacaggctt ttagttttga tggaaaaagc gaggctggct ctgtactcgg
     5101 cgatggcgag tatcaagccc tttttcaagc aaactttaac aacgggcaaa tagcaaagat
     5161 gagtcgaaat tttattatcg atacaagggc acctcaggca agtcttgttt tagaaacgcc
     5221 tattttctcg cctgatggag atggcaatct cgacctggca attatagcgc aaagtgtttc
     5281 gaaagaaaaa aactggcagg ctgaaatcct cgatgccaat cgaaaaaaag tgcgtagtta
     5341 tagttttggt gacaatccac cggaaaaagt acagtgggat gggcttcacg acaatggaag
     5401 tcttgcagaa gatggcttgt atagctataa actcattgca actgacgatg cgggaaatac
     5461 cggctttgcc gaaacaaagc tttttgagct aaataccggt acgaccgaag ttattttaac
     5521 cgtgcaaaac cctgcattct ccccaaataa agatggcgta aatgatgtac ttgttttaac
     5581 acctgttgta aaaacaaaga gtgaaattgt agaatatcgt ttagacatct tcaacgaaag
     5641 aggggaaagc gtcaaaagct ttgcggataa aaaagcgctt ccacgcaatt ttcagtggga
     5701 tggacttgac gataatggaa gtctctgccc tgacgctctg tataccgcgc agctagaaac
     5761 agtttctaaa aacggaagtg aggcaaaaac actctcgcag ccaattactc tggatacgca
     5821 gtatcctact ttggaactgt cagcggacta tcttgtcttt tcaccaaacg atgatggaaa
     5881 aaaagatatc cttcctatta aaatagcggg aagtgaggaa aaccagtgga tagctgaaat
     5941 caaaaatcaa gcaaaaacca cggtaaagaa aataacttgg caaggctccc ctgcttcttt
     6001 tgattgggat ggaaaagatg aaaacaataa taccgtcgcc gatggtaatt attcccttct
     6061 cgtttcatca acagatgatg caggcaataa aacagaaaag cagataccgc ttattgttgt
     6121 agataatcgc ccaacgaagg cttttgttac cgcagagctt tcagcattct ctccaaatgc
     6181 tgatggcata aaggatgaac aggtatttac aattatgact tccttgcaag aaggaattgc
     6241 ctcttggtca ttggcgattc tctctgaaaa tggagcgagt gtgcaaaaat ggactggaaa
     6301 tgatgcaaaa tctctacctg cgaaaattaa ctgggatgga aaagattcca gtgggaaagt
     6361 tgtggaaggc agtctaactg cagacttgga gattatctat gaaaaaggcg acgaagtttt
     6421 tgccaaaaca agtagctttc tttcatcgat tacgccacca cagcttcttg taaaaacaag
     6481 tccgcagtat tttagtcccg ataacgatgg gcaagacgac gaattgttta tcaatctgag
     6541 cgcaaaaagt gctgttccct ttacctcttg gtcttttgaa ataaatgacc cacaaaatgg
     6601 gaaaaccttt tggaaaacaa gcggaaaaag tgcaattacg gagcgtataa cttgggacgg
     6661 taagagcaat actggagaac ttgtgcaatc tgcaacagat tatccttata ccttcacggt
     6721 aagtgatgat gtcgggatga cgagtcgttt tgaaggactt atttccgtcg atgttcttgt
     6781 tattcgcgtt ggtgatgtgc tcaaaattca aattccgtct attatcttcc gccccgataa
     6841 tgcagacttt aagagcaaaa aagaagttgc aaatggctta gaacaaagtg ttatcgataa
     6901 taacgtacgt gttctaaagc gaattgctga aattctaaat aaattccgtg attacaccgt
     6961 tcgtgtagaa ggtcatgcga ataatgttac gaatacagaa aaggaagaaa tagaagaatt
     7021 ggtaccgctt tcacaagctc gtgcagcctt tgtaaaagaa gagctgagaa aatacggcgt
     7081 ttcgagtgcg agactttcta cagttgggct tggtggtaga caaccagtcg taccgcatag
     7141 cgatagagat aactggtgga aaaaccgccg tgttgaattt atattaaata aataaacgtt
     7201 tacgaggggg tgctcacggc actctctttt aagcaaaaag ctacttgatg cgcgtttaac
     7261 gtttatcagg tagctttttt ttgcagtgtc ctttttgcga tattcaaggg ctaggagttt
     7321 tttggttcgc cttcaagatt ctgaaagttt gccttaattg catcgagttt ttctatcatt
     7381 tctttttttt ctgcttcact aaagcctaaa aatgcggttt ctgtgagctc ctgtgaaatt
     7441 tttgtaaggg aatcttcgta ttgtctacct ttttcgctca agcgtatctt cgtttgcctc
     7501 ttgtcctgct tcgattgttt tcgctctata agccctattt cttcaagttt tttgcataat
     7561 gcggttgtag tggatttgtc tttgtaaact aagcgtgcaa cttcgctcat tgaaagctct
     7621 tcataaacag caagttgaaa taaaatatga ccatgtgaag tagcgagttt ttctaagcct
     7681 tcgtcgctga gacgtttttt caaaaaagag gctgtgtgcg catgaacaat cgatatgaga
     7741 aaggaaatag aagatatgtc gtgtttcata ggcatatttt agcatagaaa gaatagctta
     7801 gctagattta taattgacta gatgttctta tttatactac actagaagca tgattggttt
     7861 ttatattaaa aagaattttt ttgatggttg ggataatttt ttaagcctta ttgtttttaa
     7921 tcttatttca tatgcactgc tcattggctt gttttttttg attggttttt ttgctgaaac
     7981 acctgtttta ttttttagct ttctttttat aggtatctat ctcgtaacag ttttctattt
     8041 ttctatgagt attttaacag cagaaattgc acataataaa tctgtttcaa tactttctct
     8101 ttttaaaaac atagtttttg tttggaaaga tgccttgttt ttgagtttat tgtttttagc
     8161 tctttttatt tctgcaacca ttgcgcttcc tttctattat aatcttggtg gtatttttgg
     8221 gcttgctatg tcgggcatta ttttttggct cattcttatg acccttcttg ccttgcaatg
     8281 gtttgtccct ttgtccgtag agtttaaagg cggtttcttt aaaactttaa aaaaatccta
     8341 tcttctttta atggataatg gtggctttac ttttttcctt gctatctatt ccattgtcct
     8401 ttttcttctt tctgctttgc ttgctttttt tgctcccggt ttttcaggta tttcgcttgc
     8461 acaaaataac gccttaaaat tgcgcctcta taaatacgat tggcttgaag aaaatcccga
     8521 tattgctcaa actaaggagc gaaaaaatat accctgggat gaattacttg ctgaagatga
     8581 tgagattata gggccgagaa gttttaaaag cttaattttt ccttggaaat aaaggagata
     8641 aaaattttat gtcacgtact attgttccaa aaaattatac ttcgctttta tcggttaaag
     8701 agacggaata cgcgattgtt cttataaaag attttttcca acttgcctta tcgacagagc
     8761 taaacctaac gcgcgttacc gcacctatgt ttgtcgagca agggaagggc ttaaatgatg
     8821 accttaccgg tgttgagcga gctgtcaact tccctgtaaa agatatggat gacaggcggc
     8881 ttgaaatagt gcattctctt gcaaaatgga agcgtgtaaa gctctcagac cttggtttag
     8941 agcaaggttt tggtatttat accgatatga atgcgattcg tgctgatgaa gaactcgaca
     9001 atattcactc gctctatgtt gatcaatggg attgggaact ggtgattgga gaagaaaatc
     9061 gcagtgttga gtacttaaaa gaggtcgtaa aaaaaatata tcgctgcatg aaacggacgg
     9121 agttttttct ctacgatcgc tatccgcagt tggaaatacg cttacctgaa aaaattacct
     9181 ttatacatgc acaggaattg ctagataggt atcctgacct ttctccaaaa gaaagagaaa
     9241 atgctgtggc aaaggagttt ggtgcggtct ttataattgg cattggtgca aatctttcaa
     9301 atggcatgcc tcacgacggg cgcgctcctg attatgatga ttggattaca aaaacagctg
     9361 atggaaggca aggattaaat ggggaccttc ttttgtggaa tccacttttg gactctgcct
     9421 tggaattatc gagtatgggg attcgcgttt caccagaaag cctaaaatcg caacttatcg
     9481 agagaaattg cacccaacgc gagagcttgt ttttccataa aaaattgctc aatggagaat
     9541 taccgctttg tataggcgga gggattgggc aatcgcgact ttgtatgttt tttctgcaaa
     9601 aagcccatat aggcgagaca caggtaagta cctggcctga agaaatgatc gaaatctgca
     9661 aaaaatcggg aatacaactt ttgtgacaga gcaaaaattt actgcccgtg aactagaaga
     9721 gggcttggga acttttttta cgaggggttt ttctcatatc cgtgtggaag atagctcttt
     9781 aacagaaaat aaacaggcgc ttttggcatt tttgcgctct atagcaaaaa aagaagggca
     9841 ggttttgttt gagttttttc tttctgttga aatgcttgaa aaggacatag tcaatgcact
     9901 tgcagaaaca gcttcgaccc ttgttatctc tttcaatgga ggagagcaaa agaactttgc
     9961 aaaaaaaatt gccctgctaa atgatctcgg tttgtctttt ggttttattg ttgagctaaa
    10021 tgaaaaaaac accgagacaa aaaaactttt ttctcgcctt cttgaagaaa tcgcaggtta
    10081 ttttccaaat catgtgtatt tttcttttga aaagagcttt gcttctaaac tcactgaaaa
    10141 agatgcagag cttttacgtg cgatttcaca ttgctttgaa ttattttaca cagaaggaag
    10201 ggcggttccc tggtttaaaa gtcttttgct cagcttaaag atttctgcct atgcctttat
    10261 ttcagatttt tacgagtggt ttcttttaaa taattataca cttcctaccg agacagagga
    10321 aaaatatccg tttgcaaaaa ttttaaagat gcaagagcgt tttattcagt ttaagttaga
    10381 agaaaaaaaa atatcctata tttatcccgt gatagaagat atcttgcgct tgcatgccgc
    10441 tttttcggaa gcaattgttg aaggaaaaga gactgagctt gttttacatt atagccccga
    10501 agatactttg agtccttctt cgttttattt tttacgcttt tatgatgaag tatgtgctga
    10561 aaagacagca attcgtgttt ttcttacaga agaaggacca gaatatgaaa ttttaccatt
    10621 tttcacttga cccttttttt tagtcttagt attttaagag tagaaactac aaaaaaataa
    10681 caggatatgt gatgaaagag gcagaaaaaa aggacaaaaa agccgtggag caagatgaag
    10741 cagaaacgct aaaaaaggat gacgacttgc aaaatgagct gaatgaggaa gagattcgcg
    10801 aaaaagaagt aagcgaaaca tgtgaaaaag aagaaaaaac acttgaagaa gcgtttgagt
    10861 gtttaaaaaa agaaaacgct gacttaaaag accaatactt acggaaactt gcagaattcg
    10921 ataattatcg aaaacgcatg attaaggaaa agcaagaagc aattgattat gcaaacacga
    10981 atctcttgct cgacttgctt accactctcg atgattttga ccgtgctatt caagctgcaa
    11041 gtcaaaattc aggcgaagag aaaaaaggtg ataaaaaaca ggctgaggct tctatcctcg
    11101 aaggcttatg tttgatgaac aaacaaatgc tttctatgct tgaaaacaaa tacaatctcc
    11161 tgccttttgg aaaggctggg gaagcctatg accatgacag gcatgaggca atcggttcca
    11221 tgcaaatgcc agttgccgaa ccagtccttg cagaagtata tttaaagggc tatctcttaa
    11281 aagagcgagt gattcgtcct gcaaaagtta tggtgcatat gcccgatgga acagagacgg
    11341 aaaaataaaa aagcgaagct ttgaaaagct taaaaagata gggtcaataa aagaccaaat
    11401 atacgagcaa tatgctcaaa aatatggaga atattatggg aaaaattatt ggaattgact
    11461 taggaacaac aaactcttgt gtttcagtta tggaaggcgg tgagccaatt gttattcaaa
    11521 actctgaagg tggacgaacg actccttcga ttgtcggatt taccgcaaaa ggtgagcgag
    11581 ttgtagggca acctgcaaaa aaccaaatga taacaaaccc cgaaaacaca gtctactcta
    11641 ttaaacgttt tataggacat cgatataacg agctttcagg cgatgcaaaa atggtgccct
    11701 ttaaggtgga accttacgga aatgacgatg ttcgtatcaa tgttggtggt aaacaatatt
    11761 ctacgcaaga aatttctgcc tttacgcttc aaaaaatgaa aaaaac
//
LOCUS       DAOSSW010000054        11742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_5436_length_11742_cov_6.994524, whole genome shotgun sequence.
ACCESSION   DAOSSW010000054 DAOSSW010000000
VERSION     DAOSSW010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 11742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11742
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5436_length_11742_cov_6.994524"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..1240
                     /locus_tag="PKW26_05655"
     CDS             221..1240
                     /locus_tag="PKW26_05655"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ93086.1"
                     /translation="MAQTRRYKKIEKNFVFSILNTCKQFFSAIGRFFLFIFKAGNRKL
                     TIMLVPHSQKKVLNFQTSIFALVLMFAILGGLTLSFVVYNKQTFIASREITQLRDENK
                     KTKASLDELRDESTNLMQTAKRFQASLSQSLSILGINQTTKNQNAGSSSDLASLFDLQ
                     EISQGSLQEVAEIKELTAYLESSIQPLEQIAKMLELQGSLFADIPSVWPLKGGIGHIS
                     MAFGQNKHPITGQWYIHKGLDFSTWRSGDPIIATANGQVVTADYSQDFGYYVVIKHKH
                     GFYTRYAHMSVIRARKGQFVSQGDIIGNVGNTGLSTAAHLHYEVHIGDGVVDPAKYVY
                     VKLAK"
     gene            1247..1660
                     /locus_tag="PKW26_05660"
     CDS             1247..1660
                     /locus_tag="PKW26_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOQ93087.1"
                     /translation="MALGFDDISINTLIGSGSKCTGDMSVAGFVRIDGDLEGNIETSG
                     RIIIGESARILGNVYAKQVLVGGVVLGNIVAEKSATLFSTASLIGDLISPKIQIEQDV
                     VFQGSCISLKDPERFEEAKKDWLTKISIEKRALSL"
     gene            1670..2191
                     /locus_tag="PKW26_05665"
     CDS             1670..2191
                     /locus_tag="PKW26_05665"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HOQ93088.1"
                     /translation="MVSIDPLQAALHASSQLQSPQQKTKKTDTSKKKGLFSTLLADSA
                     KEEASLSSLPPDLANLSFDEAMQVLIDSVYEAGDILKKKPYADSFVIYKKAIGEFLRF
                     VVDESYEVEDQEGVAQKNKAGIFIKKKYTIVKTVNEELDRLAKDILYNQIEQMQLVAR
                     VDGITGLLLNTFS"
     gene            2207..3145
                     /gene="ricT"
                     /locus_tag="PKW26_05670"
     CDS             2207..3145
                     /gene="ricT"
                     /locus_tag="PKW26_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOQ93089.1"
                     /translation="MSTVFEKDIDPQDADILSSLEDEFFEDGNGDNSNYEYPPNMYKL
                     SLEYSSESMYTSLKEGEVLEADSYVIINTRYGKDVALVLGPVQKPIGVKPQDIVAIER
                     VASKEDVEKIAQLKEKEAEAFKVFREKVAIHKLDMKLITTHYLFDEPKILFFFSSDNR
                     VDFRNLVKDLVSIFKMRIELRQIGVRDESRIVGGLGVCGRAFCCNAVSDKLKPVSIRM
                     AKEQNLSLNSTKISGQCGRLLCCLSYESDCYTECRKKLPQEGAIIYFEGQQCRVIEVN
                     PLAGLVRIDADDSRIVSVAKKDLSFIDGRWIAKAKN"
     gene            complement(3142..3870)
                     /locus_tag="PKW26_05675"
     CDS             complement(3142..3870)
                     /locus_tag="PKW26_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93090.1"
                     /translation="MENVELFNEYYDELFPVSEDIKEFYLEITKKYKMPTRFLRIASN
                     TGGLEYHLALEGHDVTGLEEELSLLESASRRRRYPNTALRFLNMSSLEMTHFLGEGFY
                     NVISVLHNQLLFIHDEILIKKFFFDCKKLLSQDGSLVLQLWNLEGRHPSPMLAMPTIE
                     SERAKLFYQIWQETERFSLNMDIERSNGKIYPVLTKTEIQLYRPSDIKKLAEEAGFQS
                     IAFYADFQKNPFTKESEMLLAVLS"
     gene            4051..4296
                     /locus_tag="PKW26_05680"
     CDS             4051..4296
                     /locus_tag="PKW26_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOQ93091.1"
                     /translation="MITITVCMGSSCFSRGNSINAEIIQEYIEKNNCQATVSLKGCLC
                     EGNCNKGPIIKIDDKVFYSVSPEGIEALLNHELEVKP"
     gene            4293..6041
                     /locus_tag="PKW26_05685"
     CDS             4293..6041
                     /locus_tag="PKW26_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOQ93092.1"
                     /translation="MTHISPIYTELTECQDCYKCIRQCPVKAIRVEDGHAMVVPELCI
                     LCGHCVIHCPAKAKHVRDDVPKTKQLFNLKSRVIVSLAPSFASEFPEFSVEEMIFSLK
                     QLGFWAVSETAIGADLVSQNIAKELDALQATGGKQKLFISSACPASVEYIKQYRPSFA
                     PYITETSSPLHAHARYLKEIYGDDVGIVFIGPCIAKKREADVWNIIDSALTFNDLRQW
                     FKKEGLLKKPSTPLDKTKEDFIPFRAGQGSIYPIGGGMIDSLKTYKKLENIRSLSFTG
                     IEQIRESLEGLHNESLKAPLFLELLACEGGCVNGPGTRKGSSLALKTLRVNEYAFTAA
                     DRLPETVFETERDLSGSLPIEKKEKTKHSEEDIRHALRSVGKYTREDELNCASCGYDT
                     CRLFAEAMLDNRAEKTMCVSYMRKLAQKKANGLIRAIPGGVVIANADLKIIECNTQFA
                     SLIGPDIELMFEAKPGLEGVDLQRIIPFGKYLGDVMTRSGPDRIEKEVVFGKKIFHIS
                     VFVIEKESVAGAVIEDVTSPQIQKHRIVKQAKKVIDKNLQVVQKIAFLLGENAAESES
                     ILNSIINSFSEDEEEL"
     gene            6045..6854
                     /locus_tag="PKW26_05690"
     CDS             6045..6854
                     /locus_tag="PKW26_05690"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOQ93093.1"
                     /translation="MQFFKGFSSGNETFIEVAHHQVSKYKQGAEGDVFLSQKNNKDGR
                     IITALSDGLGSGIKAGVLATLTATMAIKFVASDIPIKRAAEIIMNTLPVCKERGISYA
                     TFTLVDIEPNARVKVIEYDNPPYILIRKQTFVEPIKEFTTIERKNVKTGPKKEAIIHY
                     SHYEARPGDRLVFFSDGVTQSGMGSPHFPFGWGYNNVQSFILDIVGRKPDISARDLTR
                     EVVNQALLNDAYKAKDDITCGIIFSREPRDLLVITGPHSTQNEMPTLFALF"
     gene            6932..7267
                     /locus_tag="PKW26_05695"
     CDS             6932..7267
                     /locus_tag="PKW26_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93094.1"
                     /translation="MNLKNLDPKIPPESEMEGADLVTEGIITMAAVAEILEKNTKQDL
                     SCRNAAEKMVELFLNSDRIVFLVGTKINDAHQDPNMPVELEIRRNIIKKISVLLQEKY
                     LKEVVIHYI"
     gene            7276..7539
                     /locus_tag="PKW26_05700"
     CDS             7276..7539
                     /locus_tag="PKW26_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOQ93095.1"
                     /translation="MKNIRVSICTGTACFVMGASEIMMLEDSIPEDLQGRMNLEIEGV
                     SCLEKCTNHNRQKAPFVLINDNLLENATLAGVIDALRTIVEEE"
     gene            7545..8993
                     /locus_tag="PKW26_05705"
     CDS             7545..8993
                     /locus_tag="PKW26_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric [FeFe] hydrogenase"
                     /protein_id="HOQ93096.1"
                     /translation="MLHLNNNASKIKREILIRLTKLQLEGKLEEGAHYIPREMAPRGS
                     EPFRCCIYHDREILRQRVIARLGWGLEDYDDDKKLADYAKEALEREAPTWPMLTVLDE
                     ACNACVKTQFVVTDACQACLARPCMLNCNKKAIEIKKSRAVIDKDLCNNCGLCMTNCP
                     YNAIIKIPVPCEEACPVGAITKDENGKEVIDYHKCTFCGNCMRACPFGAMMDKSQLVD
                     VIKHMMNGKKIHAMYAPAVAAQFRSAPGQLEGALMAAGFEKVWEVAQGADITAEKEAH
                     EFEERMEEGAKMMTTSCCPAYVRAVKRHVPALESCISDTKSPMHYTAELVKKEAPDCV
                     SVFIGPCLAKRREGMDDPYVDYVLSIEEIGALFVAKNIELEKQEIMHKENNPSPTGRN
                     FAITGGVAEAVKVRLKQPEKLKAAVINGLSKAGMKQLENYGKINSGEIPATEDTPNLI
                     EVMSCEGGCIAGPSVITNPKVAAIQLKKYLGQ"
     gene            9242..>10270
                     /locus_tag="PKW26_05710"
     CDS             9242..>10270
                     /locus_tag="PKW26_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOQ93097.1"
                     /translation="MSDKNLVMIDGNTAAGHVAHALSEVVSIYPITPSSPMGEVADEY
                     SAKGKKNIWGTVPDVVEMQSEAGAAGAVHGALTTGALSTTFTASQGLLLMIPNMYKIA
                     GELTSTVFHVAARALATSSLSIFGDHQDVMACRQTGWAMLASNNVQEVMDLALISHAA
                     TLRSRVPFLHFFDGFRTSHEIQKIEEVSYEVMKKMISDELVREHRLRGLTPEKPVIRG
                     TAQNPDVYFQSREGVNKYYNAVPGIVQEEMDKFAKLTGRKYKLFDYYGAKDAEKVIVI
                     MGSGADTCEETIDALNAAGEKLGLLKVRLYRPFGAELFVQAIPQSVKALAVLDRTKEP
                     GSLGEPLYQ"
     assembly_gap    10271..10370
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10371..>11742
                     /locus_tag="PKW26_05715"
     CDS             <10371..>11742
                     /locus_tag="PKW26_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOQ93098.1"
                     /translation="AVLDRTKEPGSLGEPLYQDVVTALSEMKRDIPVVLGGRYGLGSK
                     EFTPTMVKAVFENLSGKKINKFIIGIEDDVLGQSLSYDPNWTLEEEGMNEAMFYGLGS
                     DGTVGANKNSIKIIGEAKPELNAQAYFSYDSKKSGGFTVSHLRFGQKAIRRPYLITSA
                     DFVACHKFTYLETYDMLATAKQGATFLLNSPFDAATVWKHIPVEVQKRIIDKKLNFYV
                     IDAFKIADKAGMGSRINVVMQSAYFKISGVLPEDIAVDYMKKFIDKSYGSKGKEVVQK
                     NIATIDMALSAVEKVAYPATTEGDLHMKPAMKSDDCFVSDVLNTMAIQKGDDLPVSKL
                     PEDGTFPTATTQYEKRAIAEKIPVWDPEACIQCGQCSFVCPHAVIRMKKLSDADAAKA
                     PESFLVAEIKPKAEEGRKITLKVSGEDCTGCGLCVSVCPGKNKTDPTKKAINMTDYSE
                     ELRAQEV"
BASE COUNT         3809 a         2402 c         2343 g         3088 t
ORIGIN      
        1 aggaggtttt tgtacttccg tcctagcaaa taagtacaaa aaacactaaa atcctttgtg
       61 aaacaatact aatgtaagat tttttttgtt cctgtcgata aagatagcat agtaaagtgc
      121 aatcgtcaat gttttttttc agatttcatt tgtgagtttg acagattgca tagaacaatg
      181 ctataatacc gctagtctaa aaaaataatc gaggtagaat ttggctcaaa ctcgtcgtta
      241 taaaaaaatc gaaaaaaact ttgttttttc tatactcaat acatgtaaac aatttttctc
      301 tgcgatagga aggttttttc tttttatttt taaagcagga aatcgaaaac taacaataat
      361 gttagttcct cattcacaga aaaaagtttt aaactttcaa acaagtattt tcgccctcgt
      421 tttaatgttt gccattttgg gagggcttac gctttccttt gttgtttaca ataagcaaac
      481 ttttattgcc tcaagggaaa taacacagct tcgtgatgaa aacaaaaaaa caaaggcgag
      541 tttagatgag cttcgagatg agagtaccaa tctcatgcaa actgcgaagc gttttcaagc
      601 aagtctctca cagtctcttt ctattcttgg cataaaccaa acaacaaaaa accaaaatgc
      661 gggtagctca agcgaccttg cctcgctttt cgacttgcaa gaaatatctc aaggaagctt
      721 gcaagaagta gctgaaataa aagaacttac cgcctatctt gaaagctcta ttcaaccgct
      781 agaacaaatt gcaaaaatgc tcgagcttca agggagctta tttgcagaca tcccgagtgt
      841 ttggcctcta aaaggtggca ttgggcatat ttctatggcc tttggacaaa acaaacaccc
      901 cataactggt cagtggtata ttcataaggg cttagacttt tctacgtggc gctcaggtga
      961 tcccattatt gcaaccgcaa acgggcaagt tgttaccgct gattattcgc aagattttgg
     1021 ctactatgtt gttatcaagc ataaacatgg tttttatacc cggtatgcac atatgagtgt
     1081 tattcgtgcg cgaaaaggtc agtttgtttc ccagggagac attatcggaa atgtcggcaa
     1141 cacagggctt tccactgcag cacacttgca ctacgaagta catattggtg atggtgttgt
     1201 tgaccctgca aaatatgtct acgtcaagct tgcgaagtag gtttttatgg cactcggttt
     1261 tgatgatatt tcaataaaca cactcatagg ttctggttca aaatgtacag gagatatgtc
     1321 cgtcgcaggc tttgtgcgca tcgatggaga tcttgaggga aacattgaaa cttcaggacg
     1381 cattataatc ggagaaagcg ctcgtattct tgggaatgtc tatgccaagc aggttttagt
     1441 tgggggagtt gttcttggaa atattgtcgc agaaaaaagc gcaaccttat tttctaccgc
     1501 aagcctcata ggcgatctta tttcaccaaa aattcaaatc gagcaagatg tcgtctttca
     1561 aggttcttgt atctcgctga aagacccaga gcgttttgaa gaggcaaaaa aagattggct
     1621 tacaaaaata tcgattgaaa aaagagcctt atcactgtag gatttagtta tggttagcat
     1681 tgacccatta caagcggcac ttcatgcaag ttcgcaatta caatctccac aacaaaaaac
     1741 aaaaaaaact gatacgtcga aaaaaaaagg gctgttttca acactcttag cagatagcgc
     1801 aaaggaagaa gcgtctctct cttctttgcc gccagatcta gcaaatcttt cttttgatga
     1861 agcaatgcaa gttctcatcg attccgtata tgaggcaggc gatattctca aaaaaaaacc
     1921 atacgctgat tccttcgtta tctacaaaaa agcgattggg gagtttttgc gttttgttgt
     1981 agatgaaagc tacgaagttg aagaccaaga aggtgtagca caaaaaaaca aggcaggtat
     2041 ttttataaaa aaaaaataca ccattgtaaa aacagtaaat gaagagcttg acaggctcgc
     2101 aaaagatatt ttatataatc aaatagaaca aatgcaattg gtagcacgag tcgatggtat
     2161 aactggcttg ctactcaata cattttctta aaaatagagg ttaaaaatga gtaccgtttt
     2221 tgaaaaagat atagatccac aagatgccga tattctctca tctttagaag atgagttctt
     2281 tgaggatgga aatggagata actccaatta cgaatacccc cccaatatgt acaagctttc
     2341 tctcgaatat tcaagcgagt cgatgtatac gagtttaaaa gaaggcgaag tcttggaagc
     2401 agatagttat gttattatta acacgcgata tggaaaagat gttgccctcg ttctaggacc
     2461 agtgcaaaaa cccattggtg tcaaaccaca agacattgtc gcaatcgaaa gagttgcatc
     2521 caaagaagat gttgaaaaaa tagcccagct aaaggaaaaa gaagcagagg cttttaaagt
     2581 ttttcgagaa aaagttgcaa ttcacaaact cgacatgaaa ctcatcacaa ctcactatct
     2641 ctttgatgaa ccaaaaatcc tttttttctt tagctccgac aaccgtgtcg actttcgaaa
     2701 tctcgtaaaa gacttggtga gcattttcaa aatgcgcatc gagttgcggc aaatcggtgt
     2761 acgcgatgaa tcgagaattg ttggtggact tggtgtttgt ggacgtgctt tttgttgcaa
     2821 cgctgtttct gacaaactaa aacccgtttc tattcgcatg gcaaaggagc aaaatctttc
     2881 tctcaactca acaaaaattt caggacaatg cggaagattg ctctgttgcc tatcctacga
     2941 gtccgattgc tacactgaat gcagaaaaaa actgccccaa gaaggagcaa ttatttattt
     3001 tgagggacaa caatgtcgtg ttatcgaagt aaatccgctc gctggtcttg tgcgaatcga
     3061 tgctgatgat tcgcgaattg taagcgttgc caaaaaagac ctttctttta tagacggccg
     3121 atggatagca aaagcaaaaa actaagaaag gaccgctaaa agcatctcgc tttcttttgt
     3181 aaaaggattt ttttgaaaat ctgcataaaa tgctatcgac tggaaacctg cttcttctgc
     3241 aagtttcttt atatcgctcg gacgatagag ctgtatctcg gtcttcgtta aaaccggata
     3301 tattttccca ttgcttcgct caatgtccat gttcaagcta aagcgttccg tttcttgcca
     3361 aatttggtaa aaaagctttg cccgctccga ttcaatcgta ggcattgcaa gcatcggaga
     3421 tggatgacgc ccttccaagt tccatagctg caatacaaga gagccatctt gcgacaataa
     3481 ttttttacaa tcgaaaaaaa acttttttat aagtatctca tcgtgaataa aaagaagctg
     3541 attgtgcaag acggagataa cattataaaa tccctcaccc aaaaaatgtg tcatctcaag
     3601 actcgacata tttaaaaaac gcagcgctgt gtttggatag cgccttcgtc gacttgccga
     3661 ctcaagcaaa ctcaactcct cttccaagcc cgttacatca tgaccttcca gtgcgagatg
     3721 atattccaag cctcccgtat tcgaagctat tcgcaaaaat cgcgtcggca ttttatactt
     3781 ttttgttatc tcaagataaa actctttaat atcctctgaa acaggaaaga gctcatcata
     3841 gtattcatta aataattcca cattttccat atctatcatt atattccatg caagacaata
     3901 aatctatcct tttatttaag tatcaatatt gactaaatcg ataaaatttt atagattata
     3961 agtgttgcca agaggatgat ttgacagcct tttacaataa acaacggcaa atgtcaatga
     4021 ttggtaaaaa acatattttt gaggtttata atgattacaa ttacagtatg tatgggaagt
     4081 tcctgcttta gtcgtggaaa ctccataaat gctgaaataa tacaagaata tatagaaaaa
     4141 aataattgcc aagcgaccgt ctccttaaag ggctgtctgt gcgaaggcaa ttgcaacaag
     4201 ggtccaatta taaaaattga tgacaaggtg ttttattcag taagtcccga aggcattgaa
     4261 gcattactaa accacgaact cgaggtaaaa ccgtgacaca tatttcaccc atctataccg
     4321 agctcacaga atgccaggat tgctacaagt gcattcgtca atgtcctgta aaagcaatac
     4381 gagttgaaga tggtcatgcg atggttgtcc ccgagctttg tattctctgc ggtcattgtg
     4441 ttattcactg ccccgcaaaa gcaaaacacg tacgtgatga tgtacccaaa acaaaacagc
     4501 tttttaacct aaaatcacgc gttattgttt cacttgcgcc ctcctttgct tcagagttcc
     4561 ctgagttttc tgtcgaagaa atgatttttt ctctcaagca gcttggcttt tgggctgtct
     4621 cagaaacagc aattggagca gacctcgttt cgcaaaacat tgcaaaggaa ctcgatgcct
     4681 tgcaagctac agggggaaaa caaaagctat ttatctcgag cgcctgcccc gcaagtgtcg
     4741 aatatataaa acaataccgt ccctcatttg ctccatacat aacagaaacc tcttcccctc
     4801 ttcatgcaca tgcacgatac ctcaaggaaa tctacggcga tgatgtaggt attgttttca
     4861 tcggtccctg catcgcaaaa aaacgagaag ccgatgtatg gaacatcata gactcagcgc
     4921 ttacctttaa tgacctacgg caatggttta aaaaagaggg gcttttaaag aaaccgtcta
     4981 caccactgga taaaacaaaa gaagatttta tcccctttcg agctgggcag ggaagcatct
     5041 accccatcgg tggtgggatg attgattctc taaaaaccta taaaaaactg gaaaatatac
     5101 gctcgctttc atttacggga atagagcaaa ttcgtgaatc tttagaaggg cttcataacg
     5161 agagccttaa agcaccgctt tttttggaac ttctcgcttg tgagggaggc tgcgtcaatg
     5221 gaccgggaac tcgcaagggt tcaagccttg ccctgaaaac acttcgtgta aatgaatacg
     5281 cttttacagc cgccgaccga cttcccgaaa cggtttttga aacagagcgc gaccttagcg
     5341 gcagccttcc catagaaaag aaagaaaaaa caaaacactc agaagaagat attcgtcatg
     5401 ccctacgctc ggtgggaaaa tatacacgcg aagatgaatt aaactgtgcc agttgtggct
     5461 acgatacctg tcgactcttc gccgaagcga tgcttgacaa tcgagccgaa aaaacaatgt
     5521 gcgtttccta tatgagaaaa cttgctcaaa aaaaagcaaa cggtcttata cgcgcaatcc
     5581 caggtggcgt cgtcatagca aatgccgacc taaaaataat tgaatgcaat acacaatttg
     5641 cgagtctcat cggaccagat atagaactta tgtttgaagc aaaaccgggg ctagaaggag
     5701 ttgatcttca gcgtatcatt ccttttggaa aatatctcgg cgacgttatg accagaagcg
     5761 gtccagaccg tatagaaaaa gaagtagtct ttggcaaaaa aatttttcac atatctgtat
     5821 ttgtcattga aaaagaatct gttgcaggag cagtaatcga agatgttact tctccgcaaa
     5881 tacaaaaaca ccgcatcgta aagcaggcaa aaaaagttat agataaaaat ctccaagttg
     5941 tgcaaaaaat tgcctttttg cttggagaaa atgcagcaga gtccgagtcc atattgaact
     6001 ccattatcaa ttcattttct gaagacgaag aggagctcta ggaattgcaa ttttttaagg
     6061 gttttagctc aggaaacgaa acatttatag aggttgcaca ccaccaggtg tctaaataca
     6121 aacaaggcgc cgaaggagat gtctttcttt cacaaaaaaa caataaagac gggcgcatta
     6181 taaccgctct ttcagatggg cttggctcag gaattaaagc gggggtactc gcaacattga
     6241 ccgcaaccat ggcaataaaa tttgttgcat cggatattcc cataaaacga gctgctgaaa
     6301 ttatcatgaa cacgctaccc gtttgcaaag aacggggcat tagctatgca acctttacac
     6361 tcgtcgatat agagccaaat gcacgggtaa aagttataga atacgataac cctccctata
     6421 ttttaatccg aaaacaaacc tttgttgaac cgattaagga gtttacaacc atagaacgaa
     6481 agaatgtaaa aacagggcca aaaaaagagg ctattatcca ttactcacac tacgaagcac
     6541 gtccaggaga tagacttgtt tttttctcag atggggtaac acaatcagga atgggaagcc
     6601 cgcacttccc atttggctgg ggctacaata acgtgcaatc ttttatcctc gacatagtag
     6661 gaagaaaacc agatatttcc gcccgagatc taacgcgaga agttgtaaat caagcacttc
     6721 taaatgacgc atacaaggca aaggatgata taacctgcgg tattattttt tccagagagc
     6781 cacgagattt gcttgttata acagggcccc actctaccca gaacgagatg ccgaccttgt
     6841 tcgctctttt ttgaactttg agggaaagaa aatcatttcc ggtggtacaa ccgccaatat
     6901 tgtttctcgg gaaacgggga aaaaagtaca tgtgaactta aaaaaccttg accccaagat
     6961 accaccagag tcagagatgg aaggggcaga ccttgttacc gagggaatca taacgatggc
     7021 tgctgttgcc gaaatccttg aaaaaaacac aaaacaggat ctcagctgta gaaatgccgc
     7081 agaaaaaatg gtagaacttt ttctaaactc agatagaatt gtgtttttag ttggaacaaa
     7141 aataaacgat gcgcatcaag accccaacat gcctgttgaa cttgagattc gtcgaaatat
     7201 aattaaaaaa atttctgtat tattacaaga aaaatatttg aaggaagtgg ttatccacta
     7261 tatttaggag gttttatgaa aaacattagg gtttccattt gtacaggaac agcctgtttt
     7321 gttatgggtg cttcagaaat tatgatgctc gaagattcta tccctgaaga tttacaaggg
     7381 agaatgaatc tagaaataga aggcgtttct tgtttagaaa aatgcacaaa ccacaatcgc
     7441 caaaaggctc cctttgtttt aataaatgac aatcttttgg aaaatgccac gcttgcaggc
     7501 gttatagatg ctcttagaac aattgtagaa gaggaataat aagaatgtta cacttaaata
     7561 ataatgcgtc aaaaatcaaa agagagattt tgatacgctt aacaaaatta caactggaag
     7621 gaaaactcga agaaggggcg cactatatcc cacgagaaat ggctccccgc gggagcgaac
     7681 cttttcgctg ttgtatttat catgatcgcg aaattttacg acaacgagtt atagcacggc
     7741 ttggttgggg tttagaagac tatgacgatg ataaaaaatt agccgattat gcaaaagaag
     7801 ctcttgagcg agaagcgccg acctggccca tgctcaccgt cttggacgaa gcctgtaacg
     7861 cctgtgttaa aacacagttt gttgttactg atgcgtgcca agcatgtctt gcacgtcctt
     7921 gtatgctcaa ttgcaataaa aaagctatag aaataaaaaa atctcgtgct gttatcgaca
     7981 aggacctctg caataactgc ggactttgta tgacaaactg tccctacaat gcaattataa
     8041 aaattcccgt accttgtgaa gaagcctgtc cagtaggtgc cattacaaaa gatgaaaacg
     8101 gcaaagaagt tatcgattat cataaatgta ctttttgtgg aaactgcatg agagcctgcc
     8161 cctttggtgc aatgatggat aaaagccaac tcgtcgatgt tatcaagcac atgatgaatg
     8221 gaaaaaaaat acatgcaatg tacgcccccg ctgttgcagc tcagtttcga tccgcccccg
     8281 ggcaactcga aggagctctt atggcagccg gctttgaaaa agtctgggaa gttgcacaag
     8341 gtgccgatat taccgcagaa aaagaagcgc atgagttcga agagcgcatg gaagaaggtg
     8401 ccaaaatgat gacaacctcc tgctgccctg cctatgttcg cgcggtaaaa cgccacgtgc
     8461 cagctcttga atcttgcatt tctgatacaa agagtccgat gcactacacc gccgagcttg
     8521 taaaaaaaga agctcccgat tgtgtttctg tgtttattgg tccctgttta gcaaaacgac
     8581 gcgaaggaat ggatgatccc tatgtcgatt acgtactatc gattgaagaa atcggagcgc
     8641 tctttgttgc aaaaaatatc gaactagaaa aacaagaaat tatgcacaaa gaaaataacc
     8701 caagcccaac aggaagaaac tttgcgatta ccggtggcgt tgccgaggct gttaaagtac
     8761 gcctcaagca gccagaaaaa ctaaaagcgg cggttatcaa tggattatca aaggcaggga
     8821 tgaaacaact cgaaaactat ggcaaaataa attcaggcga aattcctgca acagaagata
     8881 ctccaaacct catcgaagtt atgtcttgcg aaggcggttg tatcgcaggt ccttctgtta
     8941 ttacaaaccc aaaagttgct gcaattcaat taaaaaaata ccttggccaa taaagccaaa
     9001 atctaagctc aaaggagttt tggtttgtaa ttatacaact aaaactcctt tttttatcgt
     9061 gttttttatt taattataaa agatatctat aataatatat ccaaaaagaa ccatatcact
     9121 ttctcacact tgaaaatatg tatagataat agtacactaa taagacaaaa acaacagaaa
     9181 gctttcctat atttaataca ttaagaaagt ttttctgaaa taaatatata aggacggttt
     9241 tatgtcggat aaaaatctgg tcatgatcga cggtaataca gccgctggtc acgttgccca
     9301 cgctttaagc gaggtagtgt ctatttaccc tatcactcct tcgagcccta tgggagaggt
     9361 tgctgacgag tattcagcaa aaggcaagaa aaacatttgg ggtactgtcc ctgatgttgt
     9421 agaaatgcaa tcagaagcag gtgcagctgg tgccgtgcac ggtgcattaa ctactggtgc
     9481 tttaagcaca acttttacag cctcacaagg gcttctttta atgattccaa acatgtacaa
     9541 aatagctgga gagcttacaa gcactgtttt ccatgttgca gctcgtgctt tagcaacaag
     9601 ttctctttca atttttggtg atcatcaaga cgtaatggct tgccgacaaa ctggttgggc
     9661 tatgcttgct tcaaataacg tacaagaagt tatggacctc gctctcatct cccatgcagc
     9721 aaccctacga agcagagttc ccttccttca cttctttgac ggttttagaa cttcacatga
     9781 aattcaaaaa atcgaagaag tttcttatga agttatgaaa aaaatgattt ctgatgaact
     9841 cgttcgtgaa caccgtctcc gtggactaac accagaaaaa cctgttattc gcggaacagc
     9901 acaaaacccc gatgtctact tccaaagccg agaaggcgta aacaaatact acaatgcagt
     9961 tcctggcatt gtacaagaag aaatggacaa atttgctaaa cttaccggcc gaaaatacaa
    10021 gctctttgac tactacggcg caaaagatgc agaaaaagtt atcgttatca tgggatcggg
    10081 cgcagacacc tgtgaagaaa caatcgatgc attaaatgca gcaggcgaaa aactaggtct
    10141 cttaaaagta cgcctctacc gacccttcgg tgcagagctt tttgtacaag caattccaca
    10201 aagcgtaaag gcccttgctg ttcttgaccg aacaaaagag cctggctcac ttggagaacc
    10261 cttgtaccaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgctgttctt
    10381 gaccgaacaa aagagcctgg ctcacttgga gaacccttgt accaagacgt ggttaccgcg
    10441 ctttcagaaa tgaaaagaga tatccctgtc gttctcggtg gtcgatacgg actcggttca
    10501 aaagaattta ccccaaccat ggtaaaagct gtttttgaaa acctcagcgg caaaaaaata
    10561 aacaagttta tcatcggtat cgaagacgac gttctcggac aaagcctaag ctacgaccca
    10621 aactggacac tcgaagaaga aggcatgaac gaagcaatgt tctacggact tggttctgac
    10681 ggtactgttg gtgcaaataa aaactcaatt aaaattattg gggaagcaaa gcctgagctc
    10741 aacgcacaag catatttttc ctacgactcc aaaaaatctg gaggctttac cgtttcacac
    10801 ttgcgatttg gtcaaaaggc aattcgacgc ccctacctca tcactagtgc tgactttgtt
    10861 gcctgccata agtttaccta ccttgaaacc tacgacatgc tcgcaacagc aaaacaaggt
    10921 gcaaccttcc ttctcaacag ccccttcgac gcagcaacag tatggaagca tattcccgtt
    10981 gaagtgcaaa aacgcattat cgataaaaag ctaaacttct acgtcataga tgcattcaaa
    11041 attgcagata aggcaggaat gggaagccgc atcaacgttg ttatgcaatc agcatacttt
    11101 aagatttctg gcgttcttcc agaagacatt gctgtcgatt acatgaaaaa attcatcgac
    11161 aaatcctacg gttccaaggg aaaagaagtt gttcaaaaaa atattgcaac catcgacatg
    11221 gcgctctcag ctgttgaaaa ggtagcttat cctgcaacaa cagaaggcga ccttcacatg
    11281 aaacctgcaa tgaaatctga tgattgcttt gtatcagatg tactcaacac aatggcaatt
    11341 caaaaaggcg acgaccttcc agtgagcaag ctcccagaag acggaacctt cccaactgcc
    11401 acaacgcagt atgaaaaacg agcaattgca gaaaaaatcc ccgtatggga tccagaagct
    11461 tgtatccaat gtggacaatg ttcctttgtt tgtcctcatg ctgttatccg catgaaaaaa
    11521 ctcagcgacg ctgacgcagc aaaagcccca gaaagcttcc ttgttgcaga aatcaagcca
    11581 aaggcagaag aagggcgaaa gattaccctc aaggtttctg gcgaagactg tactggttgt
    11641 ggactctgtg tttctgtttg tcctggaaaa aacaaaacag acccaacaaa aaaagcaatc
    11701 aatatgaccg actacagcga agaacttcga gcacaagaag tt
//
LOCUS       DAOSSW010000055        11711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_5450_length_11711_cov_6.113332, whole genome shotgun sequence.
ACCESSION   DAOSSW010000055 DAOSSW010000000
VERSION     DAOSSW010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 11711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11711
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5450_length_11711_cov_6.113332"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1688
                     /locus_tag="PKW26_05720"
     CDS             <1..1688
                     /locus_tag="PKW26_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOQ93099.1"
                     /translation="LHHGFMHPVSLFFGILSTATSVGITARILSDKKKMDSPEGVTTL
                     AAAVFDDVLGIVLLAIVLGIVTVMESSSGGSLDGKVIGLIALKAFGIWLGFTILGLVF
                     SKQIAGFLKLFKHSYDFSIAALGIGLLLAGVFEKQGLAMIIGAYVTGLSISKTDIAAV
                     IQERIHGLYEFFVPLFFCVMGMMVNVGEMLNMQVLVIGLIYTLVAVLAKVLGCGIPAL
                     SLGFNIKGAVRIGVGMLPRGEVALIIAGIGLSAGILNQQMFGITILMTLITTVLAAPL
                     LNAALNIKGRGTRKEAKADTNVSLKWDFKNDEIADLVVDSLLKNLKDEGFYVQMMNID
                     DGISQARKNDIALSIIETESLVSIETSAEDAAFVKTSVYEVLLKLTESMENLTSGFDP
                     LALKKEMAGASARMDPSLAKMLDKNTISINLEATTKDEILKELVLLATNSGHVIDWQL
                     ALKDVIAREKSMSTGMQKGIALPHAKTDGVIAPCIAIGIKKDGIDFESLDQEASRLFV
                     LILSPKKTSSPHIQVLSTMASVLHKSENLETLISAKSPQKVKDYFLGMAKTSK"
     assembly_gap    1712..1811
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1828..2220)
                     /locus_tag="PKW26_05725"
     CDS             complement(1828..2220)
                     /locus_tag="PKW26_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93100.1"
                     /translation="MLMDVRPLNANSYQNLISSGVGAGKLYVNVEPNMVLYTQYKHIS
                     GVAAQKTQHSVSVSKVQILNALIERLSKLKKQDYDIEIPGKLDDDQIDILIDDLQNKI
                     TAALELAQANPFAQGIGGDLGQLVNLVA"
     gene            complement(2262..3569)
                     /locus_tag="PKW26_05730"
     CDS             complement(2262..3569)
                     /locus_tag="PKW26_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93101.1"
                     /translation="MEDKKKKTKTRFFSKYSFFFICLLIPLSLSLGLTSCKRVYKNPV
                     VIWTDRAEFASYAELFNTSQEVCKVSIVYKKNLAHLFPLAKGEPFPDLLIGSWLKNSQ
                     TKKITSSLDYMFSDLKLNRSVFYTQLLESGQINDAQYLIPVSFNLPTILFSKENANLI
                     SENYLLSPDQIRDIAAEYNEKKDGRTYNKMGYGLNWNQDFLYEIAKLQNVDFKEQGRS
                     FSWDDEALKKTLAYMQDWTETANDSTNSEKDFAFKYLYTPFYQQVHQKRSLFAYTHSN
                     RFLDLPVDKIEALDFRWLQGNQKIPVIDDIVYLGMYRHSKNTDAASIFINWFFKEATQ
                     KQILERQVKMNLKTSTFGIADGFSSIKSVTERVFPIFYPILLKNLPSPESLIAPNMYP
                     PRWDAIRETIIKPYLLEASESPEPEKKILSLEERIKDWTKQNY"
     gene            complement(3559..>4368)
                     /locus_tag="PKW26_05735"
     CDS             complement(3559..>4368)
                     /locus_tag="PKW26_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ93102.1"
                     /translation="DSFSGGFNLETFTKALEEEAKTGIVKIILNFPQNPSGYSPSVKE
                     AEDICALIKSFAEKGTKMLIWCDDAYFGLAYEEEIDRQSLFAYLSDIHENVVAVKIDG
                     PTKEDYVWGFRTGFLTFGGKGLSDAHFAAIEKKLMGAIRSSVSCSNTASQSIMLKMFQ
                     EPSLEDEKKALKEILKGRYKRVKAFLETKKDHEILRPLPFNSGYFMSFRCEGIDAEAL
                     RQVLLHKREIGTISIDERHLRVAFSSVDKRDIETVFSAIYEEAENLKQNGR"
     assembly_gap    4369..4468
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4469..4731)
                     /locus_tag="PKW26_05740"
     CDS             complement(<4469..4731)
                     /locus_tag="PKW26_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HOQ93103.1"
                     /translation="MLHFLAQELNEILQDSPVDSFLSALGRRLYFPKGIIAQSAEAKQ
                     QGKKANATVGITVNDGKPAILPCIQKHFPYLNSSETVAYAPTAG"
     gene            4799..5827
                     /locus_tag="PKW26_05745"
     CDS             4799..5827
                     /locus_tag="PKW26_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOQ93104.1"
                     /translation="MSDIKKNPLFLEVKDILDNAKKEMAKRIVGQEEVVEGVLTALVA
                     GGHVLLEGVPGLAKTLIIKSFSDISGLDFKRIQFTPDLLPADLVGTLMYDQQNGQFYP
                     RKGPVFTNLLLADEINRAPAKVQSALLEAMAEGQVTIGDTTHILSEPFFVMATQNPIE
                     QEGTYNLPEAELDRFLLKILVNYPSAEEEMKIVKNGKDPLKIPVNIVFTSENLEKCKA
                     ALSKVHCDDKIIEYIVSLVAVTRPETEKKSAPLRVSSATASIKKGDDILRYISFGASP
                     RASIALCLCAKVQALFESRDFVLPEDVKKAALHVLRHRLVLSYEAAADGISADALISK
                     ILTYMPTP"
     gene            5919..6815
                     /locus_tag="PKW26_05750"
     CDS             5919..6815
                     /locus_tag="PKW26_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOQ93105.1"
                     /translation="MKKNKDEIQYTSLQKKAMHLRLQSLSLSQGLKQGSFQSLYKSQG
                     IEFSGVREYLPGDDIRSIDWNVTARMGRTFVKTYEEERDLHVFIILDASLSMATGTNG
                     LSRLEKAKEIASLLSFACEHISSPLGSVIFGASILFSCGQKSGKEHTMYLLSKISKTG
                     LDDTRGSALTNALRGAANLLKRRSLVFVISDFRVGDYEGDLSRLALKHDLVAIRLSDE
                     SDFHFPSMGSVPFVDPESLERQDFPLSSASFKKAWRDYGMAHIELWRQMCKKRGVKTI
                     EMSLQDDSLLVLERFFSVVRKK"
     gene            6812..7840
                     /locus_tag="PKW26_05755"
     CDS             6812..7840
                     /locus_tag="PKW26_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93106.1"
                     /translation="MKKKSLFFLFLLSFQILCPPKNTKTWAEEGFTDNSLSFYISPKE
                     SYVGDEVQLYYEFFPPADFPSFFLTNKIFLEKPQFSLFGSYDCELQSIEIVQTHKKGL
                     EKSYALSIYFIPFVTGVIEIPEFDLRELLAHEPQKTKEDFGASLIPIKIPPFEIQNIL
                     SIYPDSSLRPMRGIILLPGTSVFVYLLLAILIGISIFLFYIGYAAKKIRFSFFGLWKR
                     LFYFFYVFGIKRKIKKLLKKNIRLGEFSQIVHRYLCLYLTKRFGIDFSAATNTEILQT
                     IRMSQEGLLSEKEETILFDIQTLFIRLDFLRFSLEGPENKGEEEREKKERVFQLLGLI
                     AEMESQHV"
     gene            7833..8834
                     /locus_tag="PKW26_05760"
     CDS             7833..8834
                     /locus_tag="PKW26_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOQ93107.1"
                     /translation="MFEFENIFALLFLFCLPLYIVLKKKGFLSKPSFYLSFSDWRGSS
                     FSWKNPLYKISHLLTKLSFYLAFILVVLALGNPSLTRQEKVYTSSGTKILFVLDTSPS
                     MAALDMAGVSRLELAKEIIAVVVSQMKGTAFGLVAMGSDALSLVSPTEDHRFFMDQLY
                     QIEIGDLGDGTAIGVGISAAMYHLGSSYTSSRCIVLLTDGENNGGFIHPLTATSLAAE
                     ENIVIHTIGLGTIGTVPIEYTDAQTGKKYSGYLESRFDDVLLRAIASESGGSYFRADS
                     AALLKDALMQIVKEEEISQTYYMEEKKEYFYEKFLYSAFAFLVFAWIIKRFYLKEIL"
     gene            8831..10492
                     /locus_tag="PKW26_05765"
     CDS             8831..10492
                     /locus_tag="PKW26_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOQ93108.1"
                     /translation="MIQFERPLLLFCILLLFPALLFFSLYARKFFQNLLFIEQKNLPG
                     KSRVKVKIVLRSVFFSLAWICFVFALSGPSWGMKAEAVQKSGVALSFVFDISHSMEAL
                     DSRMGTGSLGEEKSRLSHASDWATALLSKITDKNQNPNVSVVITKGESFVSLPFSQDY
                     HSFYSYVSILNPYLMTAPGSHLGSGIRSATRSFPANHARFASIILLTDGDDTRGDLEE
                     SISEALSYGINVLIVGFGSLEGTEVLSGGSRQRQGEGTEVYTSLKEASLNEIVSRLKA
                     KYPLHSNSVHYFPARDASSLEKLASIVNPKRDSGDKMLTSYTVKPIQRHLECLFFSLV
                     FFIFALVFSQWNFKKQEKALSILSLFILFPLFFTSCSFPMKDAALLLEGAFYTNQDAY
                     DKATFSFIKALERADQDENDFLKQYALYNLSYTYMKQNEHESAQKRLKQIDVDAPDQL
                     LFARAYNQGIIAYEKGEFSEAFSYFKAALLVQPKNRDAKINLELALQQRAEVKPLGAS
                     SLSELTESNEPSEAHKSVFTLIRENEENHWKNRVVSNGDEDVLDY"
     gene            10506..>11711
                     /locus_tag="PKW26_05770"
     CDS             10506..>11711
                     /locus_tag="PKW26_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93109.1"
                     /translation="MLFCFLWFFALYALDIDLFRLVQKNAFTNETLVFRTIIEGVPPT
                     QIDLTVQSLPQNVSFVGSKKEKIIYNGLESSSVELHFIFSKSGDYKLSSVPIRIRYGY
                     QYLGFEAITVTDNPKTLQPSLLYSVYEKEGEGDRGSARDRGLEKIARGQRVEVLVEAQ
                     YFASIQSFSWQANEKTIVFNEKLLQDLPYREMDFSTKPIELLRFDFIALEEGDLLLPH
                     LLVQGKSWAGIDVQVSENEQSIYVSEAAPKEEERGTEDFALGTEDEDLGEGAFSFAAS
                     GHFGLTDSEKEALITDLEKTLQQKKALCSLVSLFSLFLSIFFFILWFIYRVRKKKKTF
                     LLPLALSIFICYFLISGITRQDWAVFEGNYFYAIPEENTKVLRVVEKPVAVRLLLETN
                     DWAHIAYPIL"
BASE COUNT         3272 a         2022 c         2464 g         3753 t
ORIGIN      
        1 tattgcatca tggatttatg catccggtaa gcttgttttt tggtattctt tcaacggcaa
       61 cctccgttgg tattaccgct cgtattttat cagataagaa aaagatggac tctccggaag
      121 gggtaacgac ccttgctgct gctgtttttg atgacgtgct tggtatcgtt ttgcttgcta
      181 ttgtccttgg tattgtaacc gttatggaat caagcagtgg tggtagttta gatggaaaag
      241 tgatagggct tattgctttg aaagcctttg gtatctggct tggatttacg attctcggct
      301 tagtattttc taaacaaata gccgggtttt taaaattatt taagcattcc tatgattttt
      361 ctatagcagc tcttggtatt ggcttattgt tagcaggagt ttttgaaaag caaggactcg
      421 cgatgattat cggtgcctat gtgacgggct tgtctatttc aaagacagat attgcagcgg
      481 ttattcaaga gcgtatacac ggtttatatg aattttttgt gccgcttttt ttctgtgtta
      541 tgggcatgat ggtcaatgtg ggcgaaatgc tcaacatgca agtgctcgtt attggcttaa
      601 tttacacact cgttgctgtc ttagcaaagg tgcttggctg tgggattcct gccttgagct
      661 taggttttaa tataaagggt gctgtgcgaa tcggggtggg tatgctacca cgaggagaag
      721 tcgctcttat tattgcaggg attggtttaa gtgcgggtat attaaatcag caaatgtttg
      781 gtatcactat tttgatgaca cttataacga cggtacttgc agcacccttg ttaaatgctg
      841 ccctcaatat aaaagggcgc ggtacacgaa aagaagcaaa ggctgatact aatgtctcac
      901 tcaagtggga tttcaaaaat gatgaaatag cggaccttgt agtggattca ttgcttaaaa
      961 atcttaaaga cgaaggtttt tatgtacaaa tgatgaatat tgacgatggt atttcacaag
     1021 cgcgaaaaaa cgacattgcc ttatcgatca ttgaaacaga atcacttgtt tcgattgaaa
     1081 cgtctgcaga agatgctgcc tttgtaaaaa catctgtgta cgaagttttg ctgaaactga
     1141 ctgaatcaat ggaaaatctt acctctggct ttgatcctct tgccttaaaa aaagaaatgg
     1201 cgggggcttc tgctcgaatg gatccaagtt tagctaaaat gcttgataaa aatactattt
     1261 caataaatct tgaggcgact acgaaggatg aaattttaaa agaattagtt ttacttgcga
     1321 caaattcagg acatgtaata gattggcaac ttgctttaaa agatgttatt gcacgggaaa
     1381 aatcgatgag taccggtatg caaaaaggca ttgcattgcc acacgcaaaa acggacggtg
     1441 ttatcgcacc ttgtatagct atagggataa aaaaagatgg gatagatttt gaatctttgg
     1501 atcaagaagc ttctcgactt tttgtattaa tcttatcccc taagaaaacg tcctcgccgc
     1561 atattcaagt gctttctacg atggcttctg tgcttcacaa aagtgagaat ttagaaacac
     1621 ttataagtgc aaaaagccct caaaaggtaa aagattattt tctcggtatg gcaaagacat
     1681 ctaaatagag aggtaaaaaa aaagctgctt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn ngaaatcctc tgtccctcta agcgacgagg ttgactaatt gccccaaatc
     1861 cccacctatt ccttgtgcaa aggggtttgc ttgggctagt tccagggcag ctgtaatttt
     1921 attttgcagg tcatcgatga gaatatctat ttggtcgtcg tcaagttttc caggtatttc
     1981 tatatcatag tcttgttttt ttagtttaga aagtcgttca atgagggcgt tcaatatctg
     2041 gaccttggat actgatactg aatgttgcgt tttttgtgct gcaactccac ttatatgctt
     2101 atattgggtg tataaaacca tatttggctc tacatttaca tacaatttgc ccgcaccaac
     2161 gccgcttgaa atgaggtttt gataagaatt tgcgtttaaa ggtcgaacat ccattaacat
     2221 aagggcctcc gatttaaata tcggaagtct atgaaaaaag attaataatt ttgcttagtc
     2281 cagtctttta tgcgctcttc gaggcttaag attttctttt caggctcagg gctttctgaa
     2341 gcttcaagta aatagggttt tataattgtt tcacgaatag catcccatcg gggaggatac
     2401 atatttgggg caattaagct ctctggggat ggaagatttt ttagtaagat gggataaaaa
     2461 atagggaaga cccgctctgt tacactcttt atagaagaaa aaccatcggc tatgccaaaa
     2521 gtagaggttt tgaggttcat ttttacttgg cgctcgagta tttgtttttg tgttgcttct
     2581 ttgaaaaacc aattgataaa aatagatgct gcatcagtgt tttttgaatg tctgtacata
     2641 ccgaggtaga caatatcatc tatgacggga attttttgat tgccttggag ccagcgaaaa
     2701 tcgagggctt ctatcttatc gacaggcaaa tctaaaaaac gatttgaatg ggtgtaggca
     2761 aaaagtgaac gtttttggtg cacttgttga taaaaaggcg tgtagagata cttaaaggca
     2821 aaatcctttt cgctatttgt cgagtcgttt gctgtttctg tccaatcttg catatatgct
     2881 aaggtttttt tgagcgcttc atcatcccaa gaaaaggatc gtccctgctc tttaaaatcg
     2941 acgttttgca gttttgctat ttcgtataaa aagtcttggt tccagtttaa accatagccc
     3001 atcttattgt aggtgcgccc gtcttttttt tcattatatt cagctgcaat atcacgtatt
     3061 tggtcaggtg aaagaagata gttttcgctt atgaggtttg cattttcttt agaaaaaagt
     3121 attgtcggta gattaaagga aacggggatg agatattgtg catcgtttat ttgtcccgat
     3181 tctaaaagtt gtgtgtaaaa aacagaacgg ttcagtttta gatcactaaa catatagtcg
     3241 agtgaggatg tgattttttt cgtttgcgaa tttttaagcc acgatccaat aagcaaatca
     3301 ggaaatggct cgccttttgc taatggaaaa aggtgagcaa ggtttttttt gtatacaatg
     3361 ctcaccttac aaacttcttg gcttgtgttg aaaagttctg catatgaggc aaactcggcc
     3421 ctatctgtcc agataacgac agggttttta taaacccgtt tgcaacttgt taaacccagg
     3481 ctgagggaca gaggaatgag gagacatata aaaaaaaagc tgtatttcga aaaaaatctt
     3541 gtttttgtct tctttttttt atcttccatt ctgcttgaga ttctcagcct cctcataaat
     3601 tgcgctaaat actgtttcta tatctcgttt gtctacgctt gaaaaggcaa ctcgaagatg
     3661 tctttcatcg atggaaatcg tgccgatttc tcttttgtgg agcaatactt ggcgtaatgc
     3721 ttctgcatcg ataccttcgc agcgaaaact cataaaatag cctgaattaa agggaagggg
     3781 gcgaagaatt tcatgatctt tttttgtttc taaaaaggct tttacccttt tgtaccgtcc
     3841 cttaagtatc tcttttaaag cttttttttc gtcttccaag cttggctctt gaaacatttt
     3901 gagcatgatg gattgtgaag cggtgttgct acaagataca gatgaacgaa ttgcgcccat
     3961 aagttttttt tcaattgctg caaaatgtgc atcgcttaag cctttcccac caaaggtcaa
     4021 aaagcctgtt cgaaagcccc aaacataatc ttctttggta ggtccatcga ttttaactgc
     4081 aacgacgttt tcatgaatat cagaaaggta ggcaaataag gattgtctgt ctatttcttc
     4141 ttcgtaggct aatccaaaat aggcatcgtc gcaccatatg agcattttcg ttcctttttc
     4201 agcaaagctt tttataagag cgcatatatc ttctgcttct ttaacactcg gagaatagcc
     4261 tgaaggattt tgagggaagt ttaaaataat ttttacgatt ccggttttag cttcttcttc
     4321 aagtgctttt gtaaaggttt ctaagttaaa accgcctgaa aaagagtcnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc tgcggttggt gcgtaggcaa ccgtttctga
     4501 agagttgaga tagggaaaat gtttttgtat acaaggtaag atcgctggtt ttccgtcatt
     4561 tacggtgata ccgacggttg cgtttgcttt tttcccctgt tgttttgctt ctgcactttg
     4621 tgcaatgata cctttaggaa agtataatcg ccttcccaaa gccgacaaaa aagaatctac
     4681 aggagagtct tgtaaaattt catttaattc ttgtgctaaa aagtgtaaca tgatagtcta
     4741 tattactata aaatatatgt ataatgtcaa tgggcaaaag agtttctaag ggggaataat
     4801 gtccgatata aagaaaaatc ctcttttttt agaggtaaaa gatatactcg ataatgcaaa
     4861 aaaagagatg gcaaagcgta ttgtcggtca agaagaggtt gtcgaagggg tcctcacagc
     4921 cttagttgca gggggacatg tgcttttgga aggtgttccg ggacttgcaa aaacgcttat
     4981 tataaaaagt ttttccgata tcagtgggct tgactttaag cgcatacagt ttactccaga
     5041 ccttcttcct gctgatttag tggggacgct catgtacgat cagcaaaatg ggcagtttta
     5101 tccgcgaaag ggacctgttt ttacaaacct cttacttgcc gatgagataa atcgtgcgcc
     5161 agcaaaggtg caatcggcct tgcttgaggc aatggcagag gggcaggtaa caatagggga
     5221 cacaacgcat attttgagcg agcctttttt tgttatggct acgcaaaacc ctatcgagca
     5281 agaaggaacc tataatctac cagaagctga actcgataga tttttactga aaatattagt
     5341 caattatccg agcgcagaag aagaaatgaa aatcgtaaaa aatggaaaag acccgctcaa
     5401 aattcccgtg aacattgtct ttacgagtga aaacttagaa aaatgcaaag cggcgctctc
     5461 taaagtgcat tgtgacgata aaatcatcga atatattgtc tctcttgttg cggtaactcg
     5521 ccctgaaaca gagaaaaaat ctgctccctt gcgagtcagc tcggcaaccg cttctataaa
     5581 gaaaggcgat gacatacttc gctatatttc ttttggtgcc tcaccccgtg caagcatcgc
     5641 gctctgtctt tgtgcgaagg tacaagcgct ttttgaaagc cgcgattttg ttttacccga
     5701 agatgtaaaa aaagccgccc tccatgtatt gcgccaccgc ctcgttcttt cctacgaagc
     5761 cgctgccgat gggataagtg ctgatgccct tatttcaaaa attttaacct atatgccaac
     5821 gccgtgagtt ttttgttcat aagcattttg gattttgatg aagagccaat gaggctctgt
     5881 ccctattggc atggtatatt cctataaaga agaaaataat gaagaaaaat aaagatgaaa
     5941 tacagtatac ctccttgcaa aaaaaagcta tgcatctacg cttgcagtcg ctttcgcttt
     6001 ctcaggggct taaacaaggc tcttttcaat ctttatataa atctcaagga atagagtttt
     6061 cgggagttcg tgagtatctt cccggagacg acattcgttc tattgattgg aatgtaacag
     6121 cgagaatggg aaggactttt gtaaaaacct atgaagaaga gagagatttg catgttttta
     6181 ttattcttga tgcttccctt tctatggcaa cggggacaaa tggtttgagt cgcttggaaa
     6241 aggcaaaaga aatagcatcc ttgttgagtt tcgcctgcga gcatatttcg agccccttag
     6301 ggtcggttat ttttggggct tcgattttat tttcatgtgg acagaaatcg ggaaaagagc
     6361 atacgatgta cttgctttca aagataagta agacgggtct tgatgatacg agaggttctg
     6421 ctttgacaaa tgcccttcgc ggggctgcaa atcttttgaa acgccgctct ttagtctttg
     6481 ttatctccga ttttcgtgtc ggagattatg aaggggatct ttcaaggctt gccctcaaac
     6541 atgaccttgt tgcaatacgc ctcagcgatg aaagcgattt tcacttccct tctatgggct
     6601 ctgtcccctt tgttgaccct gagtctttag aaagacaaga tttcccgctt tcgagcgcat
     6661 catttaaaaa agcttggagg gattatggaa tggcacatat tgaactctgg cgtcaaatgt
     6721 gcaaaaaacg tggcgttaaa acaattgaaa tgtccttaca ggatgatagt ttacttgttt
     6781 tggagcggtt tttttctgtg gtgaggaaaa aatgaaaaaa aagagcttgt tttttttgtt
     6841 tttactgagt tttcaaatcc tctgtccccc aaaaaacaca aaaacttggg cagaagaagg
     6901 tttcacggac aattctcttt ctttttatat tagtccaaag gaaagctatg ttggagatga
     6961 ggtgcaattg tactacgagt tttttcctcc tgccgatttt ccttcttttt ttctaactaa
     7021 taaaattttc ttagagaaac ctcagttttc tctttttggc tcctatgatt gtgagctaca
     7081 aagtattgag atagtccaga cgcataaaaa agggctagaa aaatcgtatg ctctatctat
     7141 ttactttatc ccctttgtaa caggggtgat agaaataccc gagtttgatt tacgtgagct
     7201 tttagcccat gaaccgcaaa agacgaagga agattttggt gcaagcctta tccccataaa
     7261 aataccgccg ttcgaaatac aaaacattct gagtatatat cctgatagca gtttgcgtcc
     7321 tatgcgtggt attattttat tgcctggaac gagtgttttt gtctatcttt tgcttgcgat
     7381 tttaatagga atcagtattt ttctttttta tataggctat gcagctaaaa aaatacgatt
     7441 ttcttttttt gggctttgga agcgtctttt ttattttttt tatgtgtttg gaataaaacg
     7501 aaaaataaaa aaactactga aaaaaaatat tcgcctaggg gagttttctc aaatcgtgca
     7561 tagatatctc tgtttgtatt taacaaagcg ctttggtatt gatttttctg cggcgacaaa
     7621 tacggaaatt ctgcaaacta ttcgaatgag tcaggaaggt ttattgagcg aaaaagaaga
     7681 aactatttta tttgacatac agactctgtt tattcggctt gactttcttc gattctcact
     7741 tgaaggaccc gagaacaaag gagaagaaga gagggagaaa aaagagcgcg tttttcagct
     7801 tttagggctt attgcagaaa tggagagtca acatgtttga atttgaaaat atttttgctc
     7861 tgttattttt attttgtctt ccactctata tcgtcttgaa aaaaaagggt tttctttcaa
     7921 agccttcttt ttatcttagt ttttctgact ggcggggttc ttctttttcc tggaaaaatc
     7981 ctctgtataa gatttcgcat ttgcttacaa agctttcttt ttatcttgcc tttatcctcg
     8041 ttgttcttgc gctgggaaat ccaagtctta caaggcaaga gaaagtctat acttcaagtg
     8101 gaactaaaat tctttttgtc ttagatacga gcccttcgat ggctgccctt gatatggcgg
     8161 gggtgagtcg ccttgagctt gccaaggaaa ttattgcagt ggttgtttcc caaatgaagg
     8221 gaacagcctt tggtttagtg gcaatgggaa gcgatgctct gtccctcgtc agtcctactg
     8281 aagaccaccg tttttttatg gaccaactat atcagataga gataggggac cttggcgatg
     8341 gtacagcgat tggcgtaggg atctcagctg cgatgtatca tcttggttcc agttatacga
     8401 gctcgcgatg tattgtgctt ttaaccgatg gggaaaataa cggtggcttt attcatcctt
     8461 tgacggcgac ttctcttgcc gctgaggaaa atattgtgat acatacgata gggcttggta
     8521 caatcggcac cgttccgata gaatataccg acgctcaaac agggaaaaaa tactcaggct
     8581 atctagagtc gcgctttgac gatgttttgt tacgtgctat tgcttctgaa agcggtggta
     8641 gctattttag agctgattct gcggccttgt taaaagatgc tctcatgcaa attgtaaaag
     8701 aggaagaaat atcacaaacc tattatatgg aagaaaagaa agagtatttt tatgaaaaat
     8761 ttttatatag cgcatttgcc tttcttgttt ttgcttggat tataaagcgt ttctatttga
     8821 aggaaatctt atgatacaat ttgaaagacc tctgttactt ttttgcattt tacttctttt
     8881 tcccgcactt ttattttttt ctttatacgc aagaaagttt tttcaaaacc tattgtttat
     8941 agagcaaaaa aatcttccag gcaaatctcg tgttaaagta aaaattgttt tgcgtagtgt
     9001 atttttttct cttgcatgga tatgttttgt atttgccttg agtggacctt cttggggaat
     9061 gaaggcagaa gctgtgcaaa aatctggagt cgcactgagc tttgtttttg acatttcgca
     9121 tagtatggaa gccctggact caaggatggg tactggtagc ttgggagaag agaagtcaag
     9181 actttcccat gcttctgact gggcaacagc tttattgtct aagataactg ataaaaatca
     9241 aaaccccaat gtttcagtgg tgataacgaa gggtgaatcc tttgttagcc tacctttttc
     9301 acaagattat cattcgtttt actcttatgt ttcgatttta aatccttatc tcatgaccgc
     9361 tccaggttct cacttgggaa gtggtattcg ctctgcaact cgctcctttc cagcaaacca
     9421 tgctcgtttt gcgagtatta tattgttgac agatggcgat gacacaaggg gcgatcttga
     9481 agaaagcatt agtgaagcgc tttcttacgg aataaatgta cttattgtag gttttggctc
     9541 tctcgaaggg acagaggtgc tttctggtgg cagtagacaa agacaggggg aagggacaga
     9601 ggtttatacg agtttaaaag aagcgtcctt gaatgaaatt gtatctcgat tgaaggcaaa
     9661 gtatccgctt cattcaaact ctgtgcatta ctttcccgct cgtgatgcaa gctctttaga
     9721 aaaacttgca tcgattgtaa atccaaaacg agattctggt gacaaaatgc ttacaagtta
     9781 taccgttaaa ccaatacaac gacatctcga atgtcttttt ttcagtttag ttttttttat
     9841 ttttgcctta gttttttcac agtggaattt taaaaaacaa gaaaaagcgc tttctatttt
     9901 gagtcttttt attttatttc ccttgttttt tacttcttgc tcttttccca tgaaagatgc
     9961 agccttgttg ctggagggag ctttctatac aaaccaagat gcctatgaca aggctacttt
    10021 ttcgtttata aaggccttgg aaagggcgga tcaagatgaa aatgattttt tgaaacagta
    10081 tgccttgtat aatctttctt atacttacat gaaacaaaat gaacatgaaa gtgcacaaaa
    10141 gcgtttgaaa caaatagatg tagatgcacc tgatcagctt ttatttgcac gtgcctataa
    10201 tcaaggtatt attgcatatg aaaagggtga gttttcagaa gctttttcgt attttaaggc
    10261 agctcttttg gtgcaaccaa aaaaccgtga tgcaaaaatc aatttagaac ttgccttaca
    10321 acaaagagct gaagtaaaac ctcttggcgc atcttctttg tcagaactta cagagtcaaa
    10381 tgaaccaagc gaggcgcata agtctgtttt tacactgata agagaaaatg aagagaatca
    10441 ttggaaaaac cgcgttgtgt caaatggaga tgaagatgtt ttggattact aagaaaaggc
    10501 tatctatgtt attttgtttt ctctggtttt ttgctttgta tgcgcttgat attgacttgt
    10561 ttcgcttagt acaaaaaaat gcctttacaa atgaaaccct cgtttttcgc actattattg
    10621 aaggcgttcc tccgacgcaa atagatctaa cggtacagag cttaccacaa aatgtctcct
    10681 ttgttggctc aaaaaaagaa aaaatcatat acaatgggct tgaatcttca agcgtggaac
    10741 tccattttat tttttcaaaa agtggcgatt ataagttaag ctctgtcccc attcgaattc
    10801 gctatggcta tcaatacctt ggttttgaag cgatcactgt aacggataat ccaaaaacac
    10861 tgcaaccgag tttgttgtat tcggtctatg aaaaagaagg ggagggggac agaggatccg
    10921 cgagggacag aggtttggaa aaaattgcac ggggacaaag ggtggaggtt cttgtagaag
    10981 cgcagtactt tgcttctatt cagtcttttt cttggcaagc aaatgagaaa acaattgttt
    11041 ttaatgaaaa acttttgcaa gaccttccgt atagggagat ggattttagt acaaagccca
    11101 ttgaactttt gcgttttgat tttattgctc tggaagaggg cgatctgctt ttgccgcatc
    11161 tccttgtaca gggaaaaagc tgggctggca tagatgtcca agtttcggaa aatgagcaaa
    11221 gcatttatgt aagtgaagcg gccccaaaag aagaggagcg agggacagag gattttgctt
    11281 tggggacaga ggatgaagac ttgggggagg gagctttttc ttttgctgca tctgggcatt
    11341 ttggcttgac ggatagtgaa aaagaggcct taatcacaga tctggaaaag actttgcaac
    11401 aaaaaaaagc attgtgtagc ttagtatccc ttttctcctt atttctaagt atctttttct
    11461 ttattctgtg gtttatctat agagtacgaa aaaagaaaaa aacatttctt ttaccccttg
    11521 ccctcagtat ttttatttgt tattttttaa taagtggtat tacaaggcaa gactgggctg
    11581 tttttgaagg aaattatttt tatgcaatcc cagaagaaaa tacaaaggta ttgcgagttg
    11641 ttgaaaaacc agttgcggtt cgtttgcttt tggaaacaaa tgattgggca catatagcct
    11701 atccgatttt a
//
LOCUS       DAOSSW010000056        11707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_5454_length_11707_cov_5.953570, whole genome shotgun sequence.
ACCESSION   DAOSSW010000056 DAOSSW010000000
VERSION     DAOSSW010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 11707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11707
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5454_length_11707_cov_5.953570"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1062)
                     /locus_tag="PKW26_05775"
     CDS             complement(115..1062)
                     /locus_tag="PKW26_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93110.1"
                     /translation="MRNTGIENKNPYSAQDYQNPNTKVVFVRVLKSLGNNRYEVSFLG
                     ERTELYSKKALEVGETFKARGFAKNGKPFLEIIEEKNAIIESFGDKDILAKLGIPTDR
                     LSELLLSFFKSLGIKINTSDLQKARALTELFEQNKEEVAEIALFLLDKGIEPSKELLA
                     SLLSQFLLEVKDTEEKESFFKATNHIKTSADHWLIFPLESFQDKNTESRENTGLIKLL
                     INTDLHITKKLSLFYLKNRQKVFIVLDYNYENGENKAKTVDFCLEPPIDSLKEKIFSS
                     KIEALLASYASVHVRYNKNLPRERFFILDSIQKNVEGWA"
     gene            complement(1149..1523)
                     /gene="rplS"
                     /locus_tag="PKW26_05780"
     CDS             complement(1149..1523)
                     /gene="rplS"
                     /locus_tag="PKW26_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOQ93111.1"
                     /translation="MDLIKTIEESQKKESLDGFKVGDTVKVHFKIIEGKNERIQIFEG
                     LVICFKNSGLSRTFTVRKNSYGVGVERIFPLHSPRVARVELVRPGKVRRAKLYYIRKK
                     IGKAAKIKELIIKKSDRVKAAK"
     gene            complement(1501..2259)
                     /gene="trmD"
                     /locus_tag="PKW26_05785"
     CDS             complement(1501..2259)
                     /gene="trmD"
                     /locus_tag="PKW26_05785"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOQ93112.1"
                     /translation="MKFHVLTLFPEIPRAFFTSSIMAKAVQKEIIAYDLVNIRDFALD
                     AHKTCDDSPYGGGAGQLLLAEPIEKALYSLKLRKKRVIYMTPSGKPFTQNYAKELSKE
                     KNLVILCGRYEGIDQRIIDSYVDDEISIGDYVLSSGEVAANVLIDAVYRLVDGVIRQE
                     SLEEESYTNNLLEYPQYTRPEVFHDMRVPEVLLSGHHKNIAQWRQKMRLIKTLKNRPD
                     LIAKARSEGNLSKEDEKMIEQIYIGEQDGPNKND"
     gene            complement(2286..2930)
                     /gene="rimM"
                     /locus_tag="PKW26_05790"
     CDS             complement(2286..2930)
                     /gene="rimM"
                     /locus_tag="PKW26_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525679.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOQ93113.1"
                     /translation="MKQSMKKPSSEQKTMNKSKDLVIGIIRGTHGLSGTMKIESTSGE
                     IDHFFSLKEVRVKKENHSKEFKVLSVFGATPLLLQLDGIESQEEAKKLVGSEILVPRD
                     MACPLYEDEYYVEDLKESALLYLPSNEANAKDASTELDRNTPILVGYITDVVEGGSGQ
                     LLEVALTESVNLLMQKPKDTEKTQKVFIPFRKEFIGTVDIAKKEVQLMHLWILE"
     gene            complement(2930..3163)
                     /locus_tag="PKW26_05795"
     CDS             complement(2930..3163)
                     /locus_tag="PKW26_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOQ93114.1"
                     /translation="MEKDLIEYMAKSLVSDPSAVSVQEVEGERGPVIELRVAAGDIGK
                     VIGKQGRIAKALRTLLSASSSKEGRRYSLEILD"
     gene            complement(3183..3431)
                     /gene="rpsP"
                     /locus_tag="PKW26_05800"
     CDS             complement(3183..3431)
                     /gene="rpsP"
                     /locus_tag="PKW26_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOQ93115.1"
                     /translation="MVKIRLKKLGSKKRPYYRIVVQDSRAPRNGACIEEIGIYHPVET
                     EEKQISFNEERAKHWLSVGAQPSDTVRKIFNKKNLTVR"
     gene            3612..>4426
                     /locus_tag="PKW26_05805"
     CDS             3612..>4426
                     /locus_tag="PKW26_05805"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOQ93116.1"
                     /translation="MFGDSHFHLSYLEERGIDLYELFTQLYQNKVPFLLDIGTHCDDL
                     FQRLDLASKLIDAFSKDKESEDALSIASFIQERLYFAAGIWPHATSILNREEELAVLS
                     SAIRKCKKEGFENISPSHLIALGECGLDHHWNKAHFDGLGLKLRLEDFLAAEAEFFEM
                     QLALAEKEALPVLVHSRDAFEATLSCIKNMGYHRGIIHCYAYGIEEARAFLDLGWYIS
                     FSGSITYAKKNKLQAYEDLVRFIPSDRLLLETDAPYLAPGKYRGKVNTPALIG"
     assembly_gap    4427..4526
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4527..4741)
                     /locus_tag="PKW26_05810"
     CDS             complement(<4527..4741)
                     /locus_tag="PKW26_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93117.1"
                     /translation="MSETDAWIEETYKDVFDEEIRGLERRCTHDPSCSIADLQGILDA
                     LYISEGNDQEGRGQRGDAIMAARIAAY"
     gene            4790..5116
                     /locus_tag="PKW26_05815"
     CDS             4790..5116
                     /locus_tag="PKW26_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HOQ93118.1"
                     /translation="MNAEEKKDESLKLLSQEGLDELIQILAAESDSVFLKDFFTCLFT
                     PSELIDIGKRWLLIKELDKGTTQREIARMFKMSLCRITRGSRELKKENSAFQKILNTY
                     NADKKA"
     gene            complement(5113..7902)
                     /locus_tag="PKW26_05820"
     CDS             complement(5113..7902)
                     /locus_tag="PKW26_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOQ93119.1"
                     /translation="MKKHLYTIAFFTLSLFLLSCVTKPKETNTKKTIQEMILSGETEE
                     AKVFFQLKSDVNAIDNDGNTVLHAAAIIDDADLVTFFIHKGAHTALKNFSGDTPLHTA
                     IKNKSIQAAKIIATADGAIFSRDGDGKSALYLGLENDALFYDALISTKTGQMRDSKNR
                     SLIHYIVEKEDTKALSFAISKKIPLSVVDDDGISPLDIAYGKTDSVESVKIASMLLLA
                     GAKPLRDSFSYFEDAVILRSISMRFEDGQTPLHLASIFGHTAIAEWIIENKALINAKD
                     ILGSTPLHEACRYGNIDSIRLLLESGADPNASDSLGKTPLHLVYENKDKATIYEMLLR
                     YGANPNVKDMYGDTPLHIASINAFDISALEVLTSYNADVNERNKQGNTPLSLAINKEN
                     KDHVAFYIRKNADIHAEDISSISPLAKVLSLKSPISLELTKLLITRDTIHSRDSFGNT
                     PLHLACEYKASPEKMQYLLSLTNDVDARNKNGDTALFLAVKKNQRQAGELLIAKKADI
                     FSTNNEHYSPLRIALEAGDERQEWLLNSESIKAKDGVGNTALHYAAEWKLDNAVVSLL
                     EKGANANVQNTNGETALFNAVKSDASTTIALLVSHKADINIRDFLGNTALHASVRYDA
                     KDAAEKLLQLKASIDAKNYAGKTPLSLAARSNRIAMANLLLDSGADVNAFDITGKTIL
                     MDAILSGRSEIIGLLLKRGASPVQQEMYGRNAFHEVAETGNPELIALIQEKAGNPLAQ
                     DFYGQTPLSIIMHKNPSLIGSILGTNRYLVDTDGNSPLHIALEQNVSTEVLDVLLDMQ
                     YPINGRNKFGITPLFIAAERNQGEAAKSLLLRGASIFLSNNEGESPLSMVLARQNSKD
                     LDDILLDIVKYARESRDHAGEAILHYAARLASIETVKGLLATGLNKEIRSNTGEKAYD
                     VAVRWDRLEIAELLR"
     gene            7992..9584
                     /locus_tag="PKW26_05825"
     CDS             7992..9584
                     /locus_tag="PKW26_05825"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ93120.1"
                     /translation="MELRLYTMDEKNIKSFEKDLVHLDAQFTLDQLDNWLTTAGLIES
                     ALSMINHRIPNAQAHLFLHSPESHEYREIIQEGLFTIPEDSLFIACLSMQDRGSSLDS
                     VFTDYSLEEPMLEEVFKSMYKSTYVIPIVHRFSLLAFILLSTEQFTQEQDSFLSEIIS
                     RLKTNLYAATVADNRQRELLELSEYPTLLHSYASLMETKKNILIDIGTKLPFDAGVYY
                     RFDEYQDLLIPEAWICGKNRPEKIERGRGISGLTLERKRSLFVPDRKTHPSFIDIKEE
                     AFIQGSFISVPVFTDKNFMGVITLSRDNEKSDAFGVEHRYMLEILAAFLSSEMTNRLL
                     YDELEDSYFSTVSSLTRALEAKDSYTKGHSERVMEFAVGAAQMLNLSFESVRKIKYAA
                     ILHDIGKIGISDTIITKDSGLTDIEYGEIKKHTEIGYDIVNDSGIFSEIKDLVRYHHE
                     KLDGSGYYKKKEGDYPWEAMIISIADIYDALTSDRPYRRAFTSKEAFASLEKLIGIHF
                     DEKIFKAFKQWIQLSTKVKKKL"
     gene            9676..10377
                     /locus_tag="PKW26_05830"
     CDS             9676..10377
                     /locus_tag="PKW26_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOQ93121.1"
                     /translation="MVYSAEVEHMCPLLQGTNHGPAPIPQEGKWVQAKEVKDISGFTH
                     GVGWCAPQQGACKLTLNVKDGIIHEALIETLGCSGMTHSAAMASEILIGKTILEALNT
                     DLVCDAINTAMRELFLQIAYGRTQSAFSEGGLVVGASLEDLGKGLRSQVGTMFATSKT
                     GPRYLEMAEGYVEQVALDADGAIIGYKFVNFGRMMDEIKKGVDAQKALEKARGTYGRT
                     TEEDGAVRFIDPRKE"
     gene            10390..11391
                     /locus_tag="PKW26_05835"
     CDS             10390..11391
                     /locus_tag="PKW26_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HOQ93122.1"
                     /translation="MAQFESYERREKQILAALNKYGISSIDETKTICDEKGVDVIKLV
                     ESIQPICFENAKWAYTVGAAMAIKKGCKTAVEVANVLGEALQAFCIPGSVAEDRKVGL
                     GHGNLAAMLLSDDANCFAFLAGHESFAAAEGAIGIARSANRARKTPLRVILNGLGKDA
                     AQIISRINGFTYVETKFDYATGKVAVVKEIPYSKGERSKVKCYGADDVREGVAIMHLE
                     HVDVSITGNSTNPTRFQHPVAGTYKKECIEQGKKYFSVASGGGTGRTLHPDNMAAGPA
                     SYGLTDTMGRMHSDAQFAGSSSVPAHVEMMGLIGMGNNPMVGASVAVAVAIAEAMNA"
BASE COUNT         3225 a         2315 c         2376 g         3691 t
ORIGIN      
        1 cctcgtgctc tttcgctatt tttagtattt gctccgctat ttctcccttg ccttgtgcag
       61 ttatgactgg tgctcttgta tttttcgtgt ataaaagagc cgttgctgtt tttttcatgc
      121 ccagccctca acgttttttt gaatggaatc aagtataaaa aaacgctctc tgggaaggtt
      181 tttattgtat ctcacatgaa cactcgcata cgaagctaaa agcgcttcga tttttgaaga
      241 aaatattttc tcctttaagc tatctatagg cggctcaagg caaaagtcca cagtctttgc
      301 tttgttttct ccattttcat agttataatc taacacaata aagacttttt gtctattttt
      361 tagataaaaa aggctgagtt ttttagtaat atgtaagtca gtgtttataa ggagtttaat
      421 gaggcctgtg ttttctctgc tttctgtgtt tttatcttga aaggattcta gtggaaaaat
      481 aagccagtga tctgcactgg tttttatgtg atttgttgcc ttaaaaaagc tttctttttc
      541 ctcggtatct tttacttcca ataaaaattg agagagtaaa gacgcgagta gttctttgct
      601 cggttcaatg cccttatcga gtagaaatag ggctatttca gcaacctctt ctttgttttg
      661 ctcaaaaagc tctgtcaaag cacgggcttt ttgtaaatca ctcgtattta tttttatacc
      721 gaggcttttg aaaaaactga gtaacaattc tgagagtcta tctgtcggga tgccgagttt
      781 tgcgagtatg tctttgtctc caaagctttc tataatggca tttttttctt caataatctc
      841 taaaaaaggc tttccgtttt ttgcaaaacc gcgtgcttta aatgtttctc cgacttcgag
      901 cgcttttttt gaatacagct cagttcgctc ccctagaaaa ctgacttcat agcgattgtt
      961 gccaagggac ttgagaacgc gtacaaaaac cacctttgtg ttgggatttt gatagtcctg
     1021 agcgctatat gggtttttat tttctatgcc tgtatttctc attgactatg agtataaaaa
     1081 aaaatgtgct agattgctag cacatttttc tttaaagtga ttgtatcacg attgctgtct
     1141 gtatgagcct attttgctgc tttaacacga tcagattttt tgataataag ctctttaatt
     1201 tttgcagcct taccaatttt tttacggata taatagagtt ttgcacggcg aaccttacct
     1261 ggtcgtacca attctactcg tgcaactcgt ggtgagtgta gggggaagat tcgctctaca
     1321 ccaacgccat aagagttttt tcttacagta aaggttcggc tcaagcctga gtttttgaag
     1381 caaataacga gaccttcgaa aatctgaatt cgctcgtttt ttccttcaat aattttaaag
     1441 tgtactttta ctgtatcacc aactttgaaa ccgtcaaggc tttctttttt ttgtgattct
     1501 tcaatcgttt ttattaggtc catcttgttc tcctatgtat atttgctcaa tcattttctc
     1561 gtcttctttg cttaaattgc cctcagatcg cgcctttgct ataaggtctg gtctgttctt
     1621 cagtgttttt ataagccgca ttttttgtcg ccactgggcg atatttttat gatgccccga
     1681 aagtaagact tctgggaccc tcatatcatg aaacacttct ggccgtgtat actgcggata
     1741 ctcaagaaga ttgtttgtat aactttcttc ctcaagcgat tcttggcgta tcactccgtc
     1801 gacgagtcta tagactgcat cgataaggac gtttgccgcc acttctccgc ttgaaagtac
     1861 ataatcacca atcgaaattt cgtcatctac atagctgtcg ataattcttt ggtctatgcc
     1921 ctcgtatctg ccacaaagaa tcacaaggtt tttttctttg ctcaattcct ttgcatagtt
     1981 ttgagtgaat ggttttccac tcggcgtcat atatataacg cgtttttttc gtagctttag
     2041 tgaataaagc gctttttcga tgggttctgc aagcaataat tgacctgcac cgccaccata
     2101 gggactgtca tcacatgttt tatgtgcatc aagggcaaaa tcccttatgt ttacaagatc
     2161 gtaggcaatg atttcctttt gaaccgcctt tgccatgatt gagctggtaa aaaaggctcg
     2221 tggtatctca ggaaataagg tcagcacatg aaattttata gaataggcca tgtataactc
     2281 ctggtttatt ccaaaatcca gaggtgcatc agttgaactt ctttttttgc aatatctacc
     2341 gttccgataa attctttacg aaagggtata aatacctttt gcgttttttc agtatccttt
     2401 ggtttttgca ttaaaaggtt cacactttcg gttaaggcaa cttctaaaag ctgacccgaa
     2461 ccgccttcca ctacgtctgt gatgtaccca acaagtatgg gggtatttct atccagttcc
     2521 gtggacgcat cttttgcatt tgcttcgttt gaaggaagat acaaaagcgc cgactctttt
     2581 aagtcttcga catagtactc atcctcatac agagggcaag ccatatcgcg tggcacaaga
     2641 atttctgaac caacaagttt ttttgcttct tcttgtgact caatgccatc taattgtagt
     2701 aaaagaggcg ttgcaccaaa aacagagagc actttgaact ctttgctatg gttttccttt
     2761 tttacgcgta cttcttttaa cgaaaaaaaa tggtctattt cgcctgaagt actttcgatt
     2821 ttcatagtac ccgataaacc atgggtccct cggataatac caatgaccaa gtccttgctt
     2881 ttattcatag ttttttgctc agagctcggt tttttcatgc tttgtttcat tagtcaagga
     2941 tttcaaggct gtatcttcta ccttcttttg aagaagaagc actgagcaag gtacgtaggg
     3001 cctttgcgat gcgtccttgt ttacctatca cttttccaat gtcaccagct gcgactcgaa
     3061 gttcaataac tggacctcgt tcgccttcta cttcttgaac tgaaacagca ctgggatcgc
     3121 tgaccaagga ttttgccatg tattcaataa ggtctttttc cattcccata ctccttcagt
     3181 tattatctta cagtaagatt ctttttgtta aagatttttc gtactgtgtc gcttggttgt
     3241 gcgccaacgc ttaaccaatg ttttgctcgc tcttcattga aagaaatttg cttttcttct
     3301 gtttcaacag gatgataaat gccgatttct tcgatgcaag cgccgtttct tggtgctcgt
     3361 gaatcttgta ctacgattcg gtagtacggt cgttttttgg agcccaattt tttcagtcta
     3421 atttttacca cttctaacct ccaagtgaat atttttttat accataaaaa agccctctag
     3481 tcaaccgaat gagggcgagg ggaggaaagg gggaaaaaaa acatagataa aacagcgtaa
     3541 acttctataa catagtatac tacgagaaat ccccccttta attttctgct tttttttgtt
     3601 aggctagtcc catgtttgga gattcacatt ttcatctatc ctacctcgaa gagcgtggga
     3661 tagatttata cgaacttttt acgcagctct atcaaaacaa ggtgcccttt ttactcgata
     3721 tagggacgca ttgtgatgac ttgtttcaac ggctcgacct cgcatcaaag ctcatcgatg
     3781 ctttttccaa ggacaaagag agtgaagacg ctttgtctat tgcttcgttt atacaagaaa
     3841 ggctttattt tgcagcaggg atttggccac atgcgacatc gattctcaat cgtgaagaag
     3901 agcttgcggt tctctcttcc gctatacgaa aatgcaaaaa agaaggtttt gaaaacattt
     3961 caccttcaca tctcattgcg ctgggagaat gcggtctcga ccatcattgg aataaggcac
     4021 attttgatgg tctcggctta aagcttcgtc tagaagattt tcttgcggcg gaagcagagt
     4081 tttttgaaat gcagcttgcg ctggctgaaa aagaagctct tcctgttctc gtgcatagtc
     4141 gggatgcctt tgaggcaacg ctttcgtgca taaaaaacat gggctatcat cgtggtatta
     4201 tccattgtta tgcctatggt atagaagagg cgagggcttt tttagatttg ggctggtata
     4261 tttctttttc tggttctata acctatgcaa agaaaaataa gttgcaggca tatgaggacc
     4321 ttgttcgctt tatcccaagc gacaggcttt tgctggaaac ggatgcaccc tatctcgcac
     4381 cgggaaaata tcgggggaag gtaaatactc ctgctcttat cggtgannnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnntcat aggctgcgat tcgtgctgcc ataattgcgt
     4561 cgcctctttg cccacgacct tcttggtcat ttccctcgct aatatacagc gcatctaaaa
     4621 taccttgtag gtctgcaatg ctacagctgg ggtcatgcgt gcagcgacgc tctaaaccac
     4681 gtatttcttc atcgaacacg tctttatatg tttcttctat ccatgcatct gtttcactca
     4741 tgcttctact atactgttaa aatttgttga tatcaagtat actgtggtta tgaatgctga
     4801 agaaaaaaaa gatgaaagtc tcaaactcct ttctcaggaa ggcttagacg aattgataca
     4861 aatccttgct gctgaaagcg attctgtttt cttaaaagat ttttttacct gtctttttac
     4921 tccatcggaa ctcatcgata ttggaaagcg gtggcttttg ataaaagagc ttgataaggg
     4981 tacaacgcaa agagaaatag cgcgaatgtt caaaatgagc ctctgtcgta taacaagagg
     5041 ctcacgtgaa ctgaaaaaag aaaactctgc atttcaaaaa atactcaata catataatgc
     5101 agataaaaag gcttagcgta aaagttctgc aatctccaat ctatcccagc gaacagctac
     5161 atcataggct ttttcgccag tattgcttct gatttctttg tttaagcctg tagctaagag
     5221 tccctttacc gtctcgatgc ttgctaggcg tgctgcatag tgtaggatag cttctcctgc
     5281 atggtctcgg ctctctcttg cgtattttac tatatcgagg aggatatcat cgaggtcctt
     5341 gctattttgt cgggcgagaa ccatggatag cgggctttct ccttcgttat ttgaaagaaa
     5401 gatagatgca ccacgtaaaa ggaggctttt tgccgcttct ccttgattgc gttcagctgc
     5461 aataaaaagc ggcgttatac caaacttatt tcgtccattt atgggatact gcatatctaa
     5521 aagcacatca agtacttctg ttgaaacatt ctgctctagt gcaatgtgca aggggctatt
     5581 gccatctgtg tcgacaagat agcgatttgt acccaaaata ctgcctatga gcgaaggatt
     5641 tttatgcata ataatcgaaa ggggagtttg tccataaaaa tcctgagcga gggggttacc
     5701 cgctttttct tggatgaggg cgatgagctc tgggtttcct gtttcggcaa cttcgtgaaa
     5761 ggcgtttctg ccatacattt cttgttgcac aggagaggcg ccgcgtttga gcaatagacc
     5821 tataatttca cttctaccac ttagaatagc gtccattaag attgtctttc ctgttatatc
     5881 aaaggcattg acatctgcgc cagagtccag taaaaggttt gccatggcga tacgattcga
     5941 ccgtgcagcg agtgagagcg gtgttttccc tgcatagttt tttgcatcga tacttgcttt
     6001 cagctgtaaa agtttttctg ccgcatcttt tgcgtcatag cgaacactcg cgtggagggc
     6061 tgtgttgccc aaaaaatctc gtatatttat atcagcctta tggctaacta acaaagcaat
     6121 cgttgtgctt gcgtcagatt ttaccgcatt gaaaagggct gtttctccgt ttgtattttg
     6181 cacatttgca tttgcaccct tttcaagcaa gctgacaact gcattgtcga gcttccactc
     6241 ggcggcatag tgtaaggcgg tatttccaac gccatctttt gccttgatgc tttccgagtt
     6301 taaaagccat tcttgtcttt catcgcctgc ttctaaagca atgcgcaagg gggaatagtg
     6361 ttcgttattt gtcgaaaaaa tatcggcttt ttttgctata agcaattctc ccgcctgtct
     6421 ttggtttttc tttaccgcaa ggaataaggc agtgtcgcca tttttatttc ttgcatcaac
     6481 atcattggtg aggctaagca aatactgcat tttttcagga gatgctttgt attcacaggc
     6541 gaggtgtagg ggagtgtttc caaaggaatc tcgagaatga attgtatctc ttgttatgag
     6601 tagtttcgtc agttcgagac ttatagggct tttaagcgat aaaacctttg caagcggaga
     6661 aatacttgaa atatcttctg catgtatatc ggcatttttt cgtatataaa aggcaacatg
     6721 gtctttattt tccttgttaa tagcaaggga aagcggggta ttgccttgtt tatttcgttc
     6781 attgacatcg gcattatatg aagtgagcac ttctaaagcc gagatgtcaa aggcatttat
     6841 gctggcaata tgcaagggag tgtcgccgta catatctttt acgtttggat ttgctccata
     6901 tcttaaaagc atttcataga ttgttgcctt atctttattt tcatagacaa ggtgcagtgg
     6961 tgttttccct aaactatcgg aagcatttgg gtctgctcca gattctagca agagtcgtat
     7021 agaatcaata ttgccataac gacaggcttc atgcagtggt gtagagccga gaatgtcttt
     7081 ggcattaata agcgccttgt tttcaataat ccactctgca attgcggtat gtccaaaaat
     7141 acttgcaaga tgcaaggggg tttgtccgtc ttcaaaacgc atagaaatac tacgtaaaat
     7201 aaccgcatct tcaaaataac taaagctatc gcgtaggggt tttgctcctg ccaaaagaag
     7261 catagaagca atttttacac tttcgacgct atctgttttc ccataggcga tatcgagggg
     7321 acttatacca tcatcatcaa cgacagaaag tgggattttt tttgaaatag caaaggaaag
     7381 tgccttagtg tcttcttttt ctactatata atggatgagc gagcgatttt tactgtcgcg
     7441 catttgtcct gtttttgttg atataagtgc atcataaaaa agagcgtcgt tttccaagcc
     7501 aagatagagg gcagattttc cgtccccatc acgagaaaaa atagctccgt ctgcagtcgc
     7561 tataattttt gccgcttgta tgctcttatt ttttatcgca gtatgaagcg gcgtatctcc
     7621 agaaaagttt tttaatgcag tatgtgctcc cttgtgaata aaaaaagtga ctaagtcagc
     7681 atcgtcgatt atggcagctg catgtaaaac agtgtttcca tcattgtcta tcgcatttac
     7741 atcactttta agctgaaaaa aaacttttgc ttcttcggtt tcacccgata aaatcatttc
     7801 ttgaattgtt ttttttgtat ttgtttcttt tggcttagtc acgcaagaca ataaaaaaag
     7861 gcttagcgta aaaaaagcta tcgtatacag gtgttttttc attttttccc ctcacaaata
     7921 aaattgagtc ttttaaagta tcgtataaaa agaaagaatg tttaggcttc ttcaattgac
     7981 atttacacaa tatggaatta agattatata ctatggacga gaaaaatata aaaagttttg
     8041 aaaaagacct tgtgcacctc gatgcacaat ttacacttga tcagcttgac aactggctca
     8101 cgacagcagg gctcattgag tcagcccttt ctatgattaa ccacagaatc cccaatgcgc
     8161 aggcgcatct ttttttgcat tcaccagaat cccacgaata cagagaaatt attcaagagg
     8221 gtttgtttac tattccagaa gactcacttt ttatagcctg tctttcgatg caagatagag
     8281 gaagcagcct cgattcggtt tttacagatt atagcctcga agagcccatg ctcgaagagg
     8341 tttttaagtc aatgtataaa tccacctatg ttatccccat cgtgcatcgt ttttctttgt
     8401 tagcctttat tttgctttca actgagcaat tcacgcaaga gcaagatagt tttctatcgg
     8461 agatcataag tcgcttaaag acaaatctct acgctgcaac cgttgccgat aaccgacaac
     8521 gagaattact tgaattgtcc gaatatccta cacttttgca cagttatgca agtttgatgg
     8581 aaactaaaaa aaatattctt atagatattg gtacaaaact cccctttgat gcgggggttt
     8641 actatcgttt tgacgagtat caagacttgc tcattcctga ggcatggatt tgtggaaaaa
     8701 atcgtcctga aaaaatagaa agagggaggg gcatatcggg tcttacactt gagagaaaac
     8761 gctctctttt tgttcctgac agaaaaacac atccgagctt tattgatata aaagaagaag
     8821 ctttcattca gggttcgttt atttcggtgc cagtttttac cgacaaaaac tttatgggcg
     8881 ttatcaccct ctctcgagac aatgaaaaaa gcgatgcctt tggagttgag catcgctaca
     8941 tgcttgaaat tcttgctgcc tttttgagta gcgagatgac caaccgcctt ctctatgacg
     9001 aacttgagga tagttatttt tcaactgttt catcacttac acgtgctctt gaggcaaaag
     9061 attcgtatac aaagggacat tcggagcgag ttatggagtt tgctgttggg gcagcccaaa
     9121 tgttaaacct ttcttttgaa tcggttcgaa aaataaaata tgctgcgata ttgcacgata
     9181 ttggcaagat tggcataagt gatacgatta taacaaagga ctcaggactc actgatatcg
     9241 aatacgggga gataaaaaaa cacactgaaa taggctacga tatcgtaaat gatagtggaa
     9301 tattttcaga aataaaagat ttagttcgat atcatcatga aaaacttgat ggctctggct
     9361 attataaaaa aaaagaaggc gattatcctt gggaggcgat gattatcagc attgcagata
     9421 tttatgatgc cttgacctca gatcgacctt atagaagagc gtttacttcc aaagaagcct
     9481 ttgcttctct tgaaaaactg ataggtattc attttgatga aaaaattttc aaagcgttca
     9541 aacaatggat acagctttct acaaaagtga aaaaaaaact ctaacgctag attaaaaaga
     9601 cttttttcat tatactgtct attatactta aaaaaaccga gtttctcggt aaattattat
     9661 ctaggagacc acaatatggt gtattctgca gaagtggaac atatgtgtcc tttactacaa
     9721 ggcacaaacc acggtcctgc tccaatccct caagagggaa aatgggttca ggcaaaggaa
     9781 gtaaaagata tttccggctt tacacatggt gtaggttggt gtgctcccca acaaggtgca
     9841 tgtaaactaa ccctcaatgt gaaagatggt attattcatg aggcacttat cgaaacttta
     9901 ggctgttcag gtatgaccca ctcagccgct atggcgagcg aaattttaat cggcaaaaca
     9961 attcttgaag ctttgaatac cgacctcgta tgcgatgcta tcaataccgc tatgagagaa
    10021 ttatttttac aaatagcgta cgggcgaaca cagtcagcct tctcagaagg tggactcgtc
    10081 gttggtgctt ctctcgaaga tttagggaag ggcttacgaa gccaagtggg aactatgttt
    10141 gcaacttcaa aaactggtcc tcgctatctt gaaatggcag aaggctatgt cgagcaagtc
    10201 gctctcgatg ctgatggtgc aatcatcgga tacaagtttg taaactttgg tagaatgatg
    10261 gatgaaataa aaaaaggcgt cgatgcacag aaagcacttg aaaaagctcg tggaacctat
    10321 ggtcgcacta ctgaagaaga tggtgcggtt cgctttatcg atccccgcaa ggaatagtga
    10381 ggtatatgta tggcacaatt tgaaagctat gaacgccgtg aaaaacaaat tcttgcggca
    10441 ttaaataaat atggtatttc atcaatcgat gaaacaaaaa caatctgcga cgaaaaaggc
    10501 gtagatgtta taaaactcgt agaatcaata cagcctattt gctttgaaaa tgcaaaatgg
    10561 gcatatactg ttggtgctgc gatggcgatt aaaaaaggct gcaaaacagc ggtagaagtt
    10621 gcaaatgttc ttggtgaagc attgcaagct ttttgtattc ctggttctgt tgctgaagat
    10681 cgaaaagttg gccttggaca tggaaacctt gccgcgatgc tcttaagcga tgatgcaaac
    10741 tgttttgcct tccttgcagg acacgaatca tttgccgcag cagaaggtgc aatcggcatt
    10801 gcgcgctctg caaaccgagc acgaaaaact cccttacgag ttattttaaa cggacttgga
    10861 aaagacgcag cacaaattat cagccgaata aacggcttta cctatgttga aacaaagttt
    10921 gactatgcaa caggtaaggt tgcggtcgta aaagaaattc cgtattcaaa gggagagcgg
    10981 tcaaaggtaa aatgctacgg cgcagacgac gttcgagaag gtgtggcaat tatgcacctc
    11041 gaacacgttg acgtatcgat tactggaaac tcaaccaacc caacccgatt ccaacacccc
    11101 gttgcaggaa cctacaaaaa agaatgcatt gagcaaggga aaaaatattt ttcagttgct
    11161 tccggcggtg gaacaggtcg aacacttcac cccgacaaca tggcagcagg accagcctca
    11221 tacggcttaa ctgacaccat gggacgaatg cactccgatg cacagtttgc aggaagctcc
    11281 tctgtaccag cccacgtaga aatgatggga ctcattggta tgggtaacaa cccaatggtt
    11341 ggcgcctctg ttgcagttgc tgttgcaatc gcagaagcaa tgaatgccta atacccttag
    11401 gagaagctcg attcatttca gcttctccta atttttttta ttgtagtact tttatttcta
    11461 aaatttcatc caagacattt tattttctca tcaaatacct tcccgatttt aattctgcat
    11521 atccaggtga attgtgatag aatggagaga ggggaagtgg gggggtggga agagggaaga
    11581 gaggtgaaaa acaactctgg aaaatgaact agccgcaaat tgcgaccggt ttgaaagaat
    11641 taaaaattca gttgtttgga agggtataat aaaagttcag tactaaaaca tccgaaaata
    11701 caattaa
//
LOCUS       DAOSSW010000057        11609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_5509_length_11609_cov_6.699931, whole genome shotgun sequence.
ACCESSION   DAOSSW010000057 DAOSSW010000000
VERSION     DAOSSW010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11609
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5509_length_11609_cov_6.699931"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PKW26_05840"
     CDS             complement(<1..558)
                     /locus_tag="PKW26_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOQ93123.1"
                     /translation="MKKNFILVVMAFLLVGMFLVAEEAVIVDFTLYDADIIADENGNP
                     TQNRRSVMDFGVAAGATFTDEQKALMRTSLSLPNWEVSLNSSARNVTSIATSTVVAAP
                     VREEAKVPFAGQSVMGVRILFPTTSVNSQAKISPPFDIPAYEPMATVDDNGDVQPQTD
                     EEKASGKTRFEEGFGVVKNVGTIKSL"
     gene            complement(639..1100)
                     /locus_tag="PKW26_05845"
     CDS             complement(639..1100)
                     /locus_tag="PKW26_05845"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOQ93124.1"
                     /translation="MIHIEKKKIVALIILASLCIISLSLFFIFNKKARFVFYFQHRDT
                     GVLQVESRYLPKVKKGDRLNLLIDELLLGPMADRSLPLFPRNTRSLSTALVGKTLYVN
                     LSSEALNILDEASTNKEGAALFRKNIFRNVRKIDIIQIYIDGSSIYENEGF"
     gene            complement(1101..2153)
                     /locus_tag="PKW26_05850"
     CDS             complement(1101..2153)
                     /locus_tag="PKW26_05850"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOQ93125.1"
                     /translation="MKSTEMKKIHSIIIFFILSSFFCLFAKVPADYVNLQDEVTKLGA
                     FLYWDPLAAHAIIQKNGHHLSISIDSSIALLDFREFHIIEPPIRKDNQVFVSKDVISL
                     AIELFQSEAELDFFKVGAILIDPGHGGKDPGAIGTHLIGGKKMTVQEKDVALATSLEL
                     FLLLKNRYPDKQVLLTRSTDVYLTLEERVEKANNINLAENEAIIYISIHANAAFDTKA
                     KGFEVWYLSPGYRRSILTETEVDKDLLPILNSMLEEEYTTESILIAKFILDSLDAEIG
                     SLSPKRGIKEEEWFVVKHAHMPSVLVELGFVTNKEEATLLADKNYLRKAAQAIYTGIS
                     AFITHFEQSRGFTGGQ"
     gene            complement(2150..3790)
                     /locus_tag="PKW26_05855"
     CDS             complement(2150..3790)
                     /locus_tag="PKW26_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOQ93126.1"
                     /translation="MGMQADTKTKKTIFILGAGFMQIPAIEAARELGLRVILSDGNPR
                     APAAHLADVFVSIDLKDSDALVDFALEQKQRGGLDAVFTAATDFSFAVAKIAEACALP
                     GHSPESARNASDKIRMRSCFQKALVPSPAFLEVHERDFSQNFSQIQASLPIAYPLVVK
                     PVDNMGSRGCRLVQNIEELYTAVEQALLYSKSKRAILEEYMDGDEFSIDALVYEGEVY
                     ITGFADRHIFYPPYFVEMGHTMPTALSREKQEEIISVFKKGVKALGLSHGAAKGDIKY
                     TKDGCKIGEIAARLSGGYMSGWTYPYASGISLTKLAIQLALGEKIPLQTMEKQFLPRL
                     HSAERAWISIPGRLKAIHGMEEAQGIENVKAVFPRAKVHDELVFPTNNVEKAGNILTQ
                     AKTRKDAIEAAENAVASLFLELEENNAETEAFLSEDFLTNFPPSAFAALGKEAAVFLE
                     KQKNEKPISVLSFINAIESKTAQKTEDFLPPFFQNCSVFDWNYKTIEKSLDDLAFFIK
                     NNIRLENYTIPAYDFWQAFLRGGSQGLLYILQKKGSSG"
     gene            complement(3813..>4300)
                     /locus_tag="PKW26_05860"
     CDS             complement(3813..>4300)
                     /locus_tag="PKW26_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOQ93127.1"
                     /translation="LAAFPQFFSSKIEAESLFPSQFGCLTMWFVIEHFENLDAVLKKV
                     NDLLVIGGVFAFSTPNGRGISMKLSKKDFFEQSPLDHFSIWHKKATKKILQAYGFKVQ
                     KIKIEESHLERIKSHRFLGFLATWYRRGGVLKWLAVCIANKLVLSDTFEVYCIKKRNI
                     L"
     assembly_gap    4301..4400
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4401..6096)
                     /locus_tag="PKW26_05865"
     CDS             complement(<4401..6096)
                     /locus_tag="PKW26_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93128.1"
                     /translation="MENLLMIIIVQARLQSSRLPNKALLPIGNLPSIAHTLLALKEIP
                     ADAHFLATDAYSYEAFLPIAKNHDYEIFSGSADNVLERFCLLIEEYLEKEAEKSLENL
                     YIVRATGDNPFLFYEAIPELLAKKDAILSQGIACDYLTFTGMPHGSGVELLKATSLLK
                     AWKRGCDAYECEHVGPALYNHKAHFSCHFIEAPPSFYAPTLRTTIDTAEDYRRALKLY
                     RHLEKKRLETGKKFLASSPCEKKVFSAREILDVLNSEAFRKPLLYIAQVKKGFGTGHF
                     RRALMVAEELDASFFVPPHASTEVKKLLEIALSEGRIFKDQILESIDSFAPGLIVLDA
                     FRTEKKEIEALAKRAPVLALDEAASEKNLRAVSYVLDTIPSLKNKKNNLMDIRLLDFR
                     SCQKKTEKIQKKTENTQGDNKTDAEKQEKTIRFLISLGGEDPGRLALKAKNLLEKAVK
                     ALQLPSEIELLLPENPIPSLKERLCNYDIVITHYGLTAFEALASGSELILLGTSKIHE
                     KLAKKYGFYNIPKNKLNQSSFEKIIQKIVFGKKVDGLGEKNLSAVFQPLLEMDFTLL"
     gene            complement(6081..7094)
                     /locus_tag="PKW26_05870"
     CDS             complement(6081..7094)
                     /locus_tag="PKW26_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93129.1"
                     /translation="MSTTPIIFNTEIARTIVGGSEKNPYASKPISVLLLSRSGSHFKP
                     QILDALMKSGFQSIVSVEKFSKNYALDELSRRFPCVRFIIPQEEINIGQMINIGMKET
                     EGETVFVVWDDIKIRENILSDFVLSKLSLFEKTLDDFTTSDNTECKIEIPRKPLCLLP
                     SLYSASSQVLPVKASPSIDDAYFTVTLSKLLYEHSISLYPYDFMGIYNKKHFFEMGGF
                     DSELSSPYWQNVEFGLRSWLWGYALFMSPAFRLSYEGERATEDMSIDDSYYRFYLKNI
                     IPRCDKGKISYPKKAFLPYAIHSGHFFKAIPAFREAKKWVESHSDKYKLDFNAILAFW
                     KTY"
     gene            complement(7069..8739)
                     /locus_tag="PKW26_05875"
     CDS             complement(7069..8739)
                     /locus_tag="PKW26_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HOQ93130.1"
                     /translation="MKRICILSAIGLHEGAFEKVFNEKSAIVLSYEWAKNLDAVEAIC
                     ILSDRPESSFSFLEKTENTSFVYKEKWTEKKLLESFFTCAEEKDAKTIIYAWADCPFL
                     QKDLSKSLLDLHEDSLSSYCFAEAFPLGLAPEIIDIDAVSLLLGLEQLKEAESAVKNT
                     RADKEEKIVAISRDLLFSLIKKDINAFEIETYISDTDMRPWRLAFSVDSKRNLVSTKS
                     LWKGILDSGFRETENSVSEIGFEKILSLSTDISVLHSLPAYYSIQIASSCAGSCLFCP
                     YPTAHFGRFRKNPQEAFYDNSAQFMEKEDFTAILDAILSFSEDALISLSLWGEALHHP
                     ELLFFIEETLKHPSLSLLIETTSYSISEDFIQSISQIIEKSPQRQTAYQAVYWILGLD
                     ARSPDLYEKLHGTGFSLEQAEKNYMLLEKAFPHSTYVQFLRIQENEAELEAFIRYWRE
                     KTDKTIIQKYNSFSGYLDEKSVLDLAPLERNPCWHLRRDMHIFLDGSVPLCHSVVPYE
                     KTEYILGNILSDNIETIWQKKKEAYGRHLENRLQKICEKCDEYYTYNF"
     gene            8802..10055
                     /gene="xseA"
                     /locus_tag="PKW26_05880"
     CDS             8802..10055
                     /gene="xseA"
                     /locus_tag="PKW26_05880"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOQ93131.1"
                     /translation="MNQESLSMNAVSLNQKENAYTVSELTSYIKELLEASFFQIVVEG
                     EISNYKPSSTGHVYFTLKDEKAAISAVIFKGKMRNIAFSPKDGNKVRLKGSLSVYALR
                     GNYQIIVDSMEEIGSGNILEMLEMRKQKLAKEGLFDEDRKKPIPFFPKRIGIVTSPTG
                     AALQDILQILQRRNPKISVVILPCPVQGSEAAAEIAKKITIANTHALCDVLIVGRGGG
                     SLEDLLPFSEEIVVRAIAASEIPVISAVGHEIDWALSDFAADKRAPTPSAAAELASPP
                     LEDILELLEQFKENFERSMSYKLDYAKALMRSFTAEGLEMQFRTIEQPLLQRFDDAKE
                     DLLLAMQYHVQEKKQALNMIQTQLQALNPLSVLERGFAIVRFKENKKTILNAQDLKLG
                     DEVEILPFKGLAEAKISQIQKREVR"
     gene            10055..10288
                     /gene="xseB"
                     /locus_tag="PKW26_05885"
     CDS             10055..10288
                     /gene="xseB"
                     /locus_tag="PKW26_05885"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOQ93132.1"
                     /translation="MAQFEKQLERLEEISASIKKSDISMEEALGFFEEGIQLAKKMEK
                     DLEKIETKIQVLMNSPVRVEDKPELDLFSLSAN"
     gene            10301..>11609
                     /gene="mutL"
                     /locus_tag="PKW26_05890"
     CDS             10301..>11609
                     /gene="mutL"
                     /locus_tag="PKW26_05890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOQ93133.1"
                     /translation="MAIFSAQRGIEKKIQKLKPELARKIAAGEVIDRPHAVVRELLDN
                     AIDSGANTIRLEIFSGGIEKIIVKDNGIGLSKEDLALCAQPHATSKVGSELDLLKIQS
                     MGFRGEALSSIAAVSRLKIQSKQRQAKENHAWCLESEVGLEDIIEPCLIHEGSIVEAQ
                     GLFANFPARRQFLKRPATEAALCRQVFIEKSLPFIDIHFSFFSDGEKKLDFPEAQSYK
                     ERFASALGLEKSLALLHELEGQDTAELPEWRFKLVIGDPSLARTDRKQIHIFTNNRKI
                     DEYSFVQAIEYGCQGYFPNGLKPIAALFLEISPALVDFNIHPAKKEARFKDSTAIHRA
                     IHTSINRFFRSFSFKAIQEDSLEREETKHQYEFSNDFSIPTRDQHFSKPAYRGKESHF
                     SPSFLGPRPSRDIERENRSSYAQASQPIKRSHIDSSFQNEQEFN"
BASE COUNT         3552 a         2286 c         2209 g         3462 t
ORIGIN      
        1 taaagattta atagtaccaa cgtttttaac aacaccgaaa ccttcttcaa accttgtttt
       61 accactggct ttttcttcat cagtttgtgg ctgtacatca ccattatcat caactgtagc
      121 cattggttca tacgcaggaa tatcaaaagg cggacttatt ttcgcttgag aatttaccga
      181 ggttgttgga aataaaattc gaacacccat aacactttga ccagcaaaag gaacttttgc
      241 ttcctctctt actggtgcag caaccactgt tgatgtagcg atacttgtta catttcgagc
      301 agaagaattc aacgatactt cccagttagg taaactgagt gaagttctca taagagcctt
      361 ctgttcatct gtaaatgtag cgccagcagc aacaccaaaa tccattacac ttcgcctgtt
      421 ttgtgtagga tttccattct cgtcagcaat aatatctgca tcataaagag taaaatcaac
      481 gatcacagct tcttcagcga cgagaaacat ccctacgagc aaaaatgcca ttacaactaa
      541 aatgaaattt tttttcattc aaagctcctt ttatacacgt tatgtagccc tgtacatatt
      601 aagtatacgc atgaaaaacg ttttgtcaat tctttttttt aaaaaccttc attttcataa
      661 atactgctac catcaatgta tatctgtatt atatctattt tcctcacatt tcgaaaaata
      721 ttttttctaa ataatgcagc accttcttta tttgttgatg cttcatcgag tatattcaat
      781 gcttcagaag aaagattcac atataaggtt ttcccaacaa gagctgtgct taaactcctc
      841 gtattgcgtg gaaacaaagg gagggatcta tcggccatag gaccaagcaa aagctcatca
      901 ataagtaagt ttagtctatc cccttttttt acctttggta gatatcgtga ttctacttgt
      961 aatacacccg tatctctatg ttgaaaataa aaaacaaaac gtgctttttt attgaatata
     1021 aaaaaaagac taagtgaaat aatgcacaag ctagcaagaa taatgagggc tactattttt
     1081 tttttctcta tatgtatcac ttattgacca cctgtaaaac ctcgtgattg ttcaaaatgc
     1141 gtaataaatg cactgattcc agtatatatt gcttgggcag ctttacgcaa gtagttttta
     1201 tctgccaaga gtgtcgcttc ttctttattg gtaacaaagc ctaattcaac caaaacactt
     1261 ggcatatggg catgtttgac aacaaaccat tcttcctctt ttatacctct ttttggagaa
     1321 agacttccaa tttcagcgtc gagggaatca agaataaatt ttgctatcaa aatgctctct
     1381 gtagtatact cttcttcgag catagagtta agtatgggaa gcaaatcctt atcaacttct
     1441 gtttctgtaa gtattgaacg acgataaccg gggcttaaat accacacttc aaaacctttt
     1501 gccttggtgt cgaatgccgc atttgcatga atagatatgt agattatcgc ttcattttct
     1561 gccaaattga tattgtttgc tttttcaacg cgctcttcga gtgttaaata tacatccgta
     1621 gatcgtgtta aaagcacttg tttatcggga tagcgatttt tcaataataa aaaaagttca
     1681 agagaggttg ccaatgcgac gtctttttct tgtacggtca tttttttacc accgatgaga
     1741 tgcgttccaa tggctcctgg gtccttacct ccgtgccctg gatcgatgag tattgcgcct
     1801 actttgaaaa aatcgagttc agcttcgctt tgaaaaagct cgattgcaag tgaaatcacg
     1861 tcttttgata caaagacttg gttatccttt cgaataggag gctcgattat atgaaattct
     1921 ctaaaatcaa gtaatgcgat gctactgtct atgctaatac ttaggtggtg tccgtttttt
     1981 tgtataatcg catgtgcagc aagtggatcc caatacaaaa aagcaccgag ttttgtcacc
     2041 tcatcttgta aattgacata gtctgcggga acttttgcaa aaaggcaaaa aaaggagctc
     2101 agaataaaaa agattatgat actgtgtatt tttttcattt ctgtcgattt caaccgctac
     2161 ttcctttttt ttgcagtata tacaataaac cttgtgaacc gccgcgcaag aatgcctgcc
     2221 aaaaatcata ggcgggaatc gtgtagtttt ctaatcttat gttatttttt ataaaaaaag
     2281 caagatcatc taagcttttt tctattgttt tataattcca atcaaaaacg gagcaatttt
     2341 gaaaaaacgg tggtaggaaa tcttctgttt tttgagctgt cttagattct attgcattga
     2401 taaaagaaag aacagagatc ggtttttcat ttttctgttt ttccaagaag actgcagcct
     2461 cttttcccaa agctgcaaag gctgagggag gaaagtttgt aagaaaatct tcgcttaaaa
     2521 aagcttctgt ctcagcatta ttttcttcaa gctcaagaaa aagacttgca acagcgtttt
     2581 cagctgcctc aatcgcatct tttcgtgttt ttgcttgcgt taagatattg ccagcttttt
     2641 ccacattatt tgtcggaaaa acaagttcgt catgtacttt tgcgcgagga aaaacagctt
     2701 ttacattttc tatgccttgg gcttcttcca tgccatgaat tgcctttaat cttccaggga
     2761 tagaaatcca agctcgctca gcactgtgca atcgaggtaa aaattgtttt tccatcgttt
     2821 gtagcggaat tttttcacct agggcaagtt gaatagctag tttagtcagc gatattccag
     2881 aggcataggg ataggtccag cctgacatat agccgcctga aagccgtgca gcaatttcgc
     2941 ctattttaca gccatctttg gtgtatttta tatcgccctt ggctgctcca tggcttaaac
     3001 ccaaggcttt tacgcctttt ttaaagactg agattatttc ctcttgtttt tctcggctca
     3061 aggctgttgg catagtatgc cccatctcaa caaaatacgg tggataaaaa atatgtcggt
     3121 ccgcaaagcc ggtaatgtat acttcgccct catacacaag cgcatcgata gaaaattcat
     3181 ctccatccat gtattcttca agaattgcac gctttgactt agagtataac aaggcttgct
     3241 cgactgcagt atataattct tctatgtttt gcacgaggcg acagccacga gaacccatat
     3301 tgtcgacggg ttttaccaca aggggatagg ctatgggaag ggaggcctgt atctgagaga
     3361 agttttggct aaaatctctt tcatgtactt ctaaaaaggc aggcgacggg acgagggctt
     3421 tttgaaagca gcttctcatg cgtattttat cgcttgcgtt tcgggctgac tctggactat
     3481 gtcctgggag agcgcaagct tcggcaattt ttgcaacagc aaaagaaaaa tctgtcgccg
     3541 cggtaaaaac tgcatctaaa ccacctcttt gtttttgctc caaggcaaaa tctacgagtg
     3601 catcgctatc ctttaagtca atcgacacaa agacatcggc aagatgagct gcaggagcgc
     3661 gtgggttgcc atcgcttagg ataacacgca agcccagctc tcgcgccgct tctatcgcag
     3721 gtatttgcat aaaacctgca ccaagtatga aaatagtttt ttttgtttta gtatctgctt
     3781 gcattcccat aattttgaag tatccacata cactataaaa tattacgctt tttgatacaa
     3841 tagacctcaa aggtatctga taaaacaagt ttatttgcaa tgcatacagc aagccatttc
     3901 aaaacaccac ctcttctata ccaagtagct aaaaaaccga gaaatctatg cgattttata
     3961 cgttcaaggt ggctttcttc tatctttatt ttttgtactt taaatccata ggcttgaagg
     4021 attttctttg tagctttttt atgccaaata ctaaaatggt ccaagggaga ttgttcaaaa
     4081 aaatcttttt tagatagttt catagaaata ccgcgtccat tgggtgtaga aaaagcaaaa
     4141 acaccgccaa tcactaaaag gtcatttact ttttttagca cagcatcgag gttttcaaaa
     4201 tgctcgatga caaaccacat cgtaagacag ccaaattgtg atggaaaaag gctttcggcc
     4261 tctatcttcg aggagaaaaa ttgaggaaag gctgctaaaa nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn ttaaaagtgt aaaatccatt tctaaaagtg gctgaaaaac
     4441 agctgaaagg tttttctccc ctagaccatc gacttttttt ccaaaaacta ttttttgaat
     4501 gattttttca aagctgcttt ggtttagctt gtttttggga atattgtaaa atccatattt
     4561 ttttgccagt ttttcatgga ttttgcttgt tcccagtaaa ataagctcag agcctgacgc
     4621 aagggcttca aaagcggtta gcccataatg cgttataaca atatcataat tgcataatcg
     4681 ctcttttaag ctcggtatgg gattttctgg caaaagaagc tcgatttcag aaggaagttg
     4741 cagcgccttc acagcctttt ctaaaagatt ttttgccttt agagcaagtc gccctgggtc
     4801 ttcaccaccg aggctaatta aaaagcgaat tgttttttct tgtttttctg catcggtttt
     4861 attatcaccc tgtgtattct ccgtcttttt ttgtatcttc tcagtttttt tttgacaaga
     4921 gcgaaaatct aagagtcgaa tatccataag gttatttttt ttatttttaa gtgaaggaat
     4981 cgtatcgagg acgtagctta ccgctcttag atttttttct gatgcagctt catcaagggc
     5041 gaggactggt gcgcgttttg ccaaggcttc tatttccttt ttttctgttc gaaaggcatc
     5101 gagcacaatg aggccgggtg caaagctgtc aattgattct aagatttggt ctttaaaaat
     5161 acgcccttca ctcaatgcta tttctaagag tttttttact tcagtggaag catggggggg
     5221 tacaaaaaag cttgcatcga gttcttctgc caccataagt gcgcggcgaa aatgtcctgt
     5281 gccaaagcct ttttttacct gcgctatgta caaaagaggc tttcgaaatg cttctgagtt
     5341 taaaacatcg agaatttctc ttgcagaaaa gacttttttt tcgcaaggag aagaagcgag
     5401 gaattttttc cctgtttcaa ggcgtttttt ttctagatga cgatagagct tcaaagccct
     5461 tcgataatct tctgcggtgt cgatggtagt gcgcaatgta ggagcataaa aagaaggcgg
     5521 tgcttcgata aaatgacatg aaaaatgcgc tttatgattg tataaggcag gtcctacatg
     5581 ctcgcattcg taggcatcgc agcctctttt ccaagccttc aaaagagaag ttgcttttaa
     5641 aagctctact ccagagccat gaggcattcc cgtaaaggta agataatcgc aggcgatgcc
     5701 ctgagagaga atagcatcct tttttgcaag gagttcaggt atcgcttcat aaaacaaaaa
     5761 aggattatcc ccagttgcac ggactatgta gagattttca aggctttttt ctgcctcttt
     5821 ttctaaatat tcctctatta aaagacaaaa acgctctaag acattatctg cacttccaga
     5881 aaaaatttca tagtcatgat tttttgcgat gggtaaaaaa gcttcatagg aataggcatc
     5941 tgttgccaaa aaatgagcat ccgctggtat ttcttttaag gctaaaagtg tatgagcgat
     6001 acttggcaga tttccgatag gcaaaagagc cttatttggg aggcggctag attgcagacg
     6061 cgcttgcacg ataataatca tcaataggtt ttccaaaaag cgagaatagc gttaaaatca
     6121 agcttgtatt tatccgaatg gctctcaacc catttttttg cctctctaaa tgctggaatt
     6181 gccttgaaaa aatgccctga atgaatggca tagggtaaaa aagctttttt tgggtaggat
     6241 atttttccct tatcgcagcg gggaatgata tttttcaaat aaaaccgata ataggagtca
     6301 tcgatgctca tatcctcggt cgctctttct ccctcatagc tgagacgaaa ggcaggagac
     6361 ataaagagtg catagcccca aagccatgaa cgtaaaccaa actcgacatt ttgccagtag
     6421 ggagaagaga gttcgctgtc aaaaccgccc atttcaaaaa aatgtttttt attgtaaatc
     6481 cccataaaat catagggata aagactaata gaatgttcat acaagagttt tgataatgta
     6541 acggtaaaat acgcgtcatc aatgcttgga cttgctttta ctggtaaaac ttgagaactt
     6601 gcagaataca gcgaaggcaa aagacaaagg ggttttcgcg gtatttctat cttacattca
     6661 gtattgtcgc ttgttgtaaa atcgtccaag gttttttcaa aaagcgatag ctttgataag
     6721 acaaagtcgc ttaaaatatt ttcacgaatt tttatatcgt cccaaacaac aaagactgtc
     6781 tcaccttcag tttccttcat gccaatattt atcatctgcc caatatttat ctcttcttgg
     6841 ggaataataa agcgcacgca gggaaaacgc cttgaaagtt catcgagggc atagttttta
     6901 gaaaattttt cgacgcttac aatcgattga aaaccgcttt tcatcaaggc gtcgagaatt
     6961 tgtggcttaa agtgggaacc agatcgagaa agcaataaaa cagaaatggg ttttgaggca
     7021 taaggatttt tttcacttcc gcctactatc gttcgtgcta tctctgtatt aaaaattata
     7081 ggtgtagtac tcatcacatt tctcacatat tttttgaagc ctattttcta aatgccgacc
     7141 gtatgcttct tttttctttt gccatattgt ttctatatta tcgcttaaaa tatttcctaa
     7201 aatatactct gttttttcat aaggtacaac tgaatgacaa agcggaacag aaccgtccaa
     7261 aaaaatatgc atgtcgcggc gaagatgcca gcaaggattt cgctccagtg gggctaaatc
     7321 aaggacagat ttttcatcaa ggtagcctga aaaactatta tatttttgaa taatcgtttt
     7381 atcggttttc tcccgccagt atcgaataaa ggcttccaat tctgcttcat tttcttgtat
     7441 acgtaaaaat tgcacatatg tagaatgcgg aaaagctttt tctaaaagca tatagttttt
     7501 ttctgcctgc tcaaggctaa aacctgtacc atggagtttt tcgtataagt caggacttcg
     7561 ggcatccaaa ccaagtatcc aatacactgc ctgatatgca gtttgtcttt gtggagattt
     7621 ttctatgatt tgacttatcg actgaataaa atcttcagaa atggaataag atgttgtttc
     7681 gattaaaaga gaaagggaag gatgttttaa agtttcttct ataaaaaata aaagttctgg
     7741 atgatgcaag gcttctcccc aaagcgagag tgaaataagg gcatcttcgg aaaaagagag
     7801 aattgcgtca agaatcgctg taaaatcttc tttttccata aactgggcag aattgtcata
     7861 aaatgcttct tgaggatttt ttctaaaacg tccaaaatgc gcagtgggat aggggcaaaa
     7921 aagacagctt cctgcacacg aacttgctat ttgtatagaa taataggctg gaaggctgtg
     7981 aaggacagaa atatcggtag aaagcgaaag aattttttca aaaccaatct ccgagacaga
     8041 gttttctgtt tctcgaaagc ctgaatcaag gatgcccttc cacaaagatt ttgttgaaac
     8101 aagattgcgt tttgaatcca ctgaaaatgc aaggcgccac ggtcgcatat ccgtatcgct
     8161 gatatatgtt tctatttcaa aggcattgat atcttttttg ataagagaaa ataaaaggtc
     8221 tcggctgata gctactattt tttcctcttt atctgcccta gtattcttta cagcgctttc
     8281 tgcctctttt aattgctcta aacccaaaag aagagaaacc gcatcgatat ctataatttc
     8341 aggagctaaa ccaaggggaa aggcttcagc aaagcaatac gaacttaaag aatcttcgtg
     8401 taagtcgagg agcgactttg aaagatcttt ttgcaaaaaa ggacaatctg cccaagcata
     8461 gataattgtt tttgcatctt tttcttctgc acaggtaaaa aaagattcga gtagtttttt
     8521 ttctgtccat ttttctttgt atacaaagga ggtgttttca gttttttcta aaaaagaaaa
     8581 agaagattct ggtctatcgc tgagaatgca aatagcttcc acagcatcga gattttttgc
     8641 ccattcataa cttaatacaa tggcagattt ttcattaaaa actttttcaa aggctccttc
     8701 gtgcaagcct attgcagata aaatacagat acgtttcata atccttttat cggcagaatt
     8761 tcgcttgcac aacagagccc ttcagtgtaa actaaaaaaa gatgaaccaa gaaagtctta
     8821 gtatgaatgc agtttccctc aaccaaaaag aaaatgccta cacggtatct gagctaacaa
     8881 gctatataaa agaacttttg gaagcgagtt tttttcaaat agtcgtagaa ggcgaaattt
     8941 caaactacaa gccttccagt accggtcatg tttattttac cctaaaagac gaaaaagccg
     9001 ctatttctgc agttattttt aaaggaaaaa tgcgaaatat tgccttttca ccaaaagacg
     9061 gaaacaaggt gcgcttaaag ggctcgcttt ctgtttacgc ccttcgagga aattatcaaa
     9121 tcatcgttga ttcgatggaa gaaattggct ctggcaatat tcttgaaatg ctcgaaatgc
     9181 gcaaacaaaa gctcgccaaa gagggtttat ttgacgaaga tcgaaaaaaa ccgattccct
     9241 ttttcccaaa acgcataggc attgtaacaa gccctactgg agcggccttg caagatattt
     9301 tgcaaatttt acaaagacga aatcccaaaa tctccgtcgt catactcccc tgccccgtgc
     9361 aaggaagcga ggctgccgca gagatagcaa aaaaaatcac aatcgcaaat actcatgcgc
     9421 tttgcgacgt actcatcgtg gggcgtggtg gcggctccct cgaagacctt ctcccttttt
     9481 ctgaagaaat agttgtacgt gccatagctg cctcagaaat tcctgtaata tctgctgtgg
     9541 ggcatgaaat agactgggcc ttaagcgatt ttgcagcgga taaaagggcg ccgacaccct
     9601 cagcggcagc cgagcttgca agtccccctt tggaagacat tctagaactt ctcgagcagt
     9661 ttaaggaaaa ctttgaacga agcatgagct ataagctcga ctatgcaaaa gcccttatga
     9721 ggtcatttac agcagagggc ttagaaatgc agtttaggac gattgagcaa cccttattac
     9781 agcgatttga cgacgcgaag gaagacttgc tccttgcgat gcagtatcac gtacaagaaa
     9841 aaaaacaggc cctgaacatg atacaaacgc aattacaagc ccttaaccca ctttctgttt
     9901 tagagcgcgg ttttgcaatt gtgcgattta aagaaaacaa aaaaacaatt ctcaatgccc
     9961 aagacctaaa actgggcgat gaagtagaaa tcctaccttt taaaggttta gcggaagcaa
    10021 aaataagtca gatacaaaag cgggaggtca gataatggct caatttgaaa aacaacttga
    10081 acggctcgaa gaaataagcg catctataaa aaaatcggat attagcatgg aagaagccct
    10141 aggatttttt gaagaaggta ttcaacttgc aaaaaaaatg gaaaaagatt tagaaaaaat
    10201 tgaaacaaag atacaagtgc ttatgaattc acctgttagg gtcgaggaca aacctgaact
    10261 cgatttattt agtctttcag ccaattaaca aagagagaaa atggcaattt tcagcgccca
    10321 aagaggcata gaaaaaaaaa tacaaaaact caagcctgag cttgcacgaa aaatagctgc
    10381 aggggaagtt atagacagac cccatgcagt tgttcgcgag ctcctcgata acgcaattga
    10441 ctcaggtgca aacactattc ggcttgaaat tttttcaggt ggcatagaaa aaataatcgt
    10501 aaaagataat ggaatcgggc tttcaaaaga agatttagct ctctgcgccc agccccatgc
    10561 gacaagtaag gttggctccg aactagactt gctcaaaatt caaagcatgg gctttcgcgg
    10621 agaggcactc tcgtcaattg ctgcagtaag ccgtttaaaa atccaatcca aacaaaggca
    10681 ggcaaaagaa aatcacgcct ggtgcttaga aagtgaagtg ggcttagaag atataattga
    10741 accctgcctt attcacgaag gaagcattgt cgaagcgcaa ggcctttttg caaactttcc
    10801 tgcgaggcgt caattcctga aacgcccagc aacagaggca gcactttgcc gacaggtttt
    10861 tatcgaaaaa agcctcccct ttatcgatat acattttagt ttttttagcg atggagaaaa
    10921 aaaactcgac tttcccgagg cacaaagcta caaagaacgc tttgcaagcg ctcttggctt
    10981 agaaaaaagc cttgcacttt tacatgaatt agaaggacag gatacagccg agctgccaga
    11041 atggcgcttt aaactcgtca taggggaccc aagccttgcc cgaacagata gaaagcaaat
    11101 acatattttt acaaataacc gaaaaattga cgaatacagc tttgtgcaag caatagaata
    11161 tggttgccaa ggctatttcc ccaatgggct caaacccatt gctgcgcttt ttttagaaat
    11221 ttctcctgcc cttgttgatt tcaatattca ccctgcaaaa aaagaagcac gctttaaaga
    11281 ttcaactgcg atacatcgtg ctattcacac cagcatcaac cgtttttttc gctctttttc
    11341 atttaaagca atccaagaag atagcctaga aagggaagaa accaaacatc aatatgaatt
    11401 ttcaaatgat ttttctatac cgacaaggga ccaacatttt tcaaagcctg cctacagggg
    11461 aaaagaaagc catttttctc cctcttttct aggtccgaga cctagcagag atattgaaag
    11521 ggaaaatcga agcagctatg ctcaggcttc tcaacctata aaaagaagcc acatagatag
    11581 ctcttttcaa aacgagcagg aatttaaca
//
LOCUS       DAOSSW010000058        10987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_5850_length_10987_cov_6.854464, whole genome shotgun sequence.
ACCESSION   DAOSSW010000058 DAOSSW010000000
VERSION     DAOSSW010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 10987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10987
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5850_length_10987_cov_6.854464"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PKW26_05895"
     CDS             complement(<1..219)
                     /locus_tag="PKW26_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93134.1"
                     /translation="MKKNVLPSIVIIVFSISIIFLFILKPKVSKSFWKDYFVLAVEKD
                     YPTEDIVHFLETASIFDFIFHLSPTRALD"
     gene            complement(226..732)
                     /locus_tag="PKW26_05900"
     CDS             complement(226..732)
                     /locus_tag="PKW26_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HOQ93135.1"
                     /translation="MALVGKIVDMFSRTHFFLNSFFSNPVLLAGIFSWFFAQFIKTVI
                     HLLTRKVKSFRDVIELLVWRTGGMPSSHSALVTSLSTSIGIRNGLESDLFILSLFFAL
                     VVIRDAVGVRRSSGIQAKTINEMGDSLSQKKVLSYRPIKEVDGHKPIEVIVGALLGVS
                     MSFAFFYL"
     gene            833..1741
                     /locus_tag="PKW26_05905"
     CDS             833..1741
                     /locus_tag="PKW26_05905"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOQ93136.1"
                     /translation="MEFLTFIAEKDDANRRFDRLISRFLPQMPTGIVQKNIRTGFIRL
                     NQKKAKPNSLIQEKDRISIARILYQEYKDTKEILKKEKLSISIKTLFKNEHLWIVHKE
                     RGMLSQKAKKNDTSLNCILKEASQHSPSLSFVPGILHRLDRETSGLIVFSQSLKGAQW
                     FSSIMQENTLGKYYIGIAEGRLLKDEIWEDTIDGERCITEVRLLEEGRYKEKNLSLIE
                     YKIITGKKHQIRKQGEKHRLSLLGDKKYGGFTDDFIQDYALHAYKLAFPENNLGIPPI
                     VKDSMPKYFQDMAEICLLKLDSPFIL"
     gene            1749..2645
                     /locus_tag="PKW26_05910"
     CDS             1749..2645
                     /locus_tag="PKW26_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93137.1"
                     /translation="MKTVAQFKTLNHIINFFKGITVYALVGESGTGKSFRAKLLAQKF
                     GLEAIIDDGLLIMDDKILAGHSAKREKTFLAAVRVALFDDKAHRDAIAKQLQKLKISK
                     ILILGTSDKMVNKIATRLQLPLPTKIIRIEDIATQDEIEYAIRSRRVEGKHVIPVPAI
                     EVRRNYPQIFYNAVKVLFKRKKTPIISGNNTKVYEKSVVRPEFSKKGRIEISEAALSQ
                     MVMHCVHEFDEEVRLKKMSIKTDERGYRLIITLDLPFGSQLTGKIHSMQKYIIDNIEQ
                     YTGILIEEVSIIIDKIRRTKKS"
     gene            complement(2642..3760)
                     /gene="pcnB"
                     /locus_tag="PKW26_05915"
     CDS             complement(2642..3760)
                     /gene="pcnB"
                     /locus_tag="PKW26_05915"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HOQ93138.1"
                     /translation="MLFRYTNDKNGNPVKKAVIYTENEHPISNASIDSDAIAVIEKLK
                     DHGFEAFIVGGAVRDLLLGNVPKDFDLVTDATPQRLKKIFRNSRIIGKRFRIVHIFFG
                     TKIFEVSTFRSICDGCSIGNDFGTMDEDVMRRDFTINALYYDPVKKQLIDYVNGVKDI
                     EKKRIRPVIQIDKIFVEDPVRMIRAVKYAALTNFSLPHNLKRQIKKSSHLLSPISPSR
                     LTEEFFKILNSGHAHAIISLSLETSLYMYLQPQATALLSESKDFEQSYMAHLQALDKL
                     VSQNQNIRQGEKLIFLVQDFVDGLTDWEKEAEEANIAELYGKTWTRVRHFVLPINPQR
                     IELEFAIKKILQSYGIRVKKPKRIKKEKQVRIKKTENS"
     gene            complement(3810..4745)
                     /locus_tag="PKW26_05920"
     CDS             complement(3810..4745)
                     /locus_tag="PKW26_05920"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ93139.1"
                     /translation="MTDKREIQRVRMSDELNIALHSWLPRGEVKKVVHISHGMAEHAP
                     RYDAFAQYLNNYGIAVYVHDHRGHGQSIQERAPAGHLADKDGFNRVTEDLREIINFTK
                     NTHKDVPYILLAHSFGSFVAQNFIASYAELIDALILSGTTANNAPLGRFSNLFARFLS
                     LFRGIRHQSPLLTKIAFGSYNKRIVNPKSKNSWLSSDDRAVEAYDADPFCGMDCTYGF
                     YRDLGYGFTIINKKAKLDAIPKNLPIFLFVGSDDPVSNYTKGLRWLYDAYKSRGLDVE
                     LRVYEGARHECLNELQKEKVYKDILSFINKIDLRK"
     gene            4813..6471
                     /locus_tag="PKW26_05925"
     CDS             4813..6471
                     /locus_tag="PKW26_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93140.1"
                     /translation="MNKTLCKQFFTLILFFPSIGFVFSQSTVLGTKSWQTVLSGNVLS
                     KIEPSSYGFVVITEGRLLNAFSENGQILWQRNLGQKGSGFCVSMNGDFVYTAIDKKLS
                     LFNTSGIALWTIETKTPIVEKPLPGWDGRLFVREKNAISSYTINGLLRFRLEVSDGLD
                     FPLVSLNDGSLLYVQKKNLNGKSTALRISPFGAILEEIIFVDTIRSLTESKNGLVLGL
                     ENGNLAVCHVVDNLFDTKWSIKIENKNILDVFFWEDSILVQFSDGSFALFDEESKEKV
                     WHTGSITKEKIEDAIFDMEKGIFRSFSRIFVINKDGSVEKNIQVQNNSRFSGLTESGF
                     FVEVKDDWSISAYRIALVENRLHKQSQIKTYPLPPVKASSAELYKNAPVLLQSINLFE
                     TEAELISRLRLDIKTIQNSYLFMEKPSQNFETLALKIKTIQNAAYLGTGEPMSFFESI
                     LDRESDESILFQCLRAIQKNAYDYDMRLLLAIEKRLVQRNQISDALLEEYCYTITEIC
                     RFMGRKTFMRKGKDLLFSFLDAKYKMAVQETARECLEKIIAFEK"
     gene            6526..8115
                     /locus_tag="PKW26_05930"
     CDS             6526..8115
                     /locus_tag="PKW26_05930"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HOQ93141.1"
                     /translation="MMIKKRAFTLLILIFFLPLFSLEVARDELRSVTETIEFINYTGP
                     HTVIQSVDEIRGIGLRLATAVNKNNLMAPGTSGSQNLYYVIHAVDPNTKEKLDADILI
                     IGENAQVDHVQNLRRILSSYLSSAYDYSRQDADTLAHFITIYNAVYRSKMDVFNAKYK
                     ALVTGYLQQEKVGLSVNYVDWPGKTQIVIPLLDLSGGLSTVDTTIISDKSVVDSMREA
                     DGKEIDVRKDMVDLKEREADAAREEAQSSQKEAVRAEEKAKEALVEQKQEEQKLAEKK
                     EEAAKAVEIAKENPEDVQAQKAAEEAEKEVVAQEQKLAEAKEKTEEAKQEAEELKTIA
                     KEEQVIADRKTAEAQQDRLDIAKDQKEVMAAEDARAQMTTSYALKVIDTKALLSALVL
                     IDVADGAVVKESPVNVIRNRQVIKTNEGYLAVAGKPGKNTTIKLVILDPKNLEIQKES
                     AEIVHESSAFTFTSNAVFVVIVQDKKTYLAAYNHDLSLSARSEVEVQDVSPIIITDRG
                     ISVIKADGKPILLDAKTLKKQ"
     gene            complement(8173..9636)
                     /gene="glgA"
                     /locus_tag="PKW26_05935"
     CDS             complement(8173..9636)
                     /gene="glgA"
                     /locus_tag="PKW26_05935"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002679256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOQ93142.1"
                     /translation="MKILMITAEAVPFAKTGGLADAVTALAIALRKTGADVRIIMPRY
                     YKIDRDKLELLDEPMAVHLSYKEEWTAVYKTDIPTGHKDYPSLPIYFIDHEQCFGRDG
                     VYGTPFESDFHDNPRRFSLLGHASFQLCHKLGWYPDVMHAHDWSASLVPALLKFNMRH
                     GVFAQTKSVLTIHNLGYQGVYPKHHYPLFGLDWSHFYDSGFEDFDRINFLKAGIRSAD
                     HLTTVSPTYAQEIQTAAYGFNLDGLLRERSYSLTGILNGVDMDYWNPKKDRYLPVNYD
                     TKTLAKKAENKRALQERMGLEVNPDIPIVGIVTRLADQKGVAELFAPTFGSAYSICRD
                     MKLQMIVLGSGESWCEDELRSLQGKLDNFRAYIGYDEGLSHLIEAGSDFFLMPSRYEP
                     CGLNQMYSLLYGTLPIVRRTGGLADTVENYNEETGEGTGFVFDNLTPGSIYDTVGWAI
                     YAWYNKKAHIEAMKLRGMKKDFSWDASAKRYLEVYGW"
     gene            9714..10892
                     /locus_tag="PKW26_05940"
     CDS             9714..10892
                     /locus_tag="PKW26_05940"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOQ93143.1"
                     /translation="MLKCNQMTHDELLIRIQNGEDSFTQFKRQAIPAKELAKEFVAFL
                     NAEGGIIVFGVEDKTNAILGLTQIEVEQIGQLIGNSANENVKPPFHPIVENIMIQGKR
                     LVIAYVPKGISKPYATNNGEFFIKSSSDKKKISQDELRRLFAESKRLFADEEIVHGTS
                     IKDLDTTSFARFLEKDNPYIYGQLQEDVLSLQQIMINLDLLKEEQLTLAGNLLFGIHP
                     QKYNKSFYIDCCHFDGIDSSVDTYKSKRKMDGPFHTLFFNSLEFLKSSLRNMQTNADF
                     NSSPQLEIDERVLSELIVNALVHRDYYILSSIKIFIFTDRVEIISPGKLTNSLTVDKI
                     KSGLSIHRNPILNSIAKTILPYSGYGSGIKRSLNILPSIDFINDIEKEQFICIIPRPR
                     "
BASE COUNT         3653 a         2093 c         2121 g         3120 t
ORIGIN      
        1 atctaaagct cgagtcggtg aaaggtgaaa aataaaatca aagatagatg ctgtttctaa
       61 aaaatgaaca atgtcttcag ttggatagtc tttttctacc gcaagaacaa aatagtcttt
      121 ccaaaaactc tttgaaactt tcggctttaa aataaataaa aaaataatgg atatagaaaa
      181 aacaataata acgatactag gtaaaacgtt ttttttcatt gtgttttata ggtaaaaaaa
      241 agcaaaactc atagaaacac ctaaaagcgc accgactatt acttctatcg gtttatgacc
      301 atcaacttct tttataggtc tatagctaag tacttttttt tgtgaaagtg aatcgcccat
      361 ttcattgatt gtttttgctt gaataccact agagcgtctc acacccactg catcgcgaat
      421 aacaacaagg gcaaaaaaaa gagagagaat aaaaagatca gactccagcc cattacgaat
      481 accgattgaa gtagaaagcg aggttaccaa agcgctatga ctcgatggca ttccgccagt
      541 gcgccataca agcaactcta taacatctcg aaagcttttc accttccttg ttaaaagatg
      601 aataaccgtt tttataaatt gtgcaaaaaa ccagctaaaa attcccgcaa gtagcactgg
      661 gtttgagaaa aaagagttta agaaaaaatg tgtgcgagaa aacatatcta ctattttgcc
      721 gactaatgcc attttgtcaa gtaggacggt ataaatgcaa tctctcctct tttctctcct
      781 cttcttgtat gaaaatgatt gacatttttt tttcctgtct tactatagaa ccatggagtt
      841 tttaacattt atagcagaaa aagatgatgc taacagacgg tttgacagac ttatttcacg
      901 ctttttacca caaatgccta caggaattgt gcaaaaaaac attagaacag gttttattcg
      961 tttaaaccag aaaaaagcaa aaccgaatag tcttatccaa gaaaaagacc gcatctcaat
     1021 tgctcgtatc ctctatcaag aatataaaga tacaaaagaa attctaaaaa aagaaaaact
     1081 tagcatatct ataaaaacac tctttaaaaa cgagcatctt tggatcgtcc ataaagaaag
     1141 aggaatgctc agtcaaaaag caaaaaaaaa cgatacgagc ttaaattgta ttttaaaaga
     1201 agcatcccaa catagccctt cactctcctt tgttccgggc attttacatc gactggatag
     1261 agaaacttca ggactcattg ttttttctca aagcctaaag ggtgctcagt ggtttagttc
     1321 cattatgcag gaaaatacac tggggaaata ttatattgga atagcagaag ggcgactcct
     1381 aaaagacgag atatgggagg atacaatcga tggggaaaga tgcattacag aggtgcgtct
     1441 tttagaagaa ggtcgctaca aagaaaaaaa tctaagcctt atagaatata aaattattac
     1501 cgggaaaaaa caccaaatac gaaagcaagg tgaaaagcat cgcttaagcc ttcttggcga
     1561 taaaaaatac ggaggtttta cagacgattt catacaagac tatgcccttc atgcctataa
     1621 acttgcattt ccagaaaata atctcggcat tccacctatc gtaaaagata gtatgccaaa
     1681 gtattttcaa gatatggcag aaatctgctt gctcaagctt gattctccct ttatactata
     1741 agaagcatat gaaaacagta gcacaattta aaaccctaaa tcacattata aattttttca
     1801 aaggaataac agtctatgcc ctcgttggag aaagcggcac gggaaaaagt ttccgagcaa
     1861 aactccttgc acaaaaattt ggcttagaag ctattatcga tgatgggctt ttgattatgg
     1921 atgataaaat ccttgccggt cattcagcaa agcgcgaaaa aacattttta gcggctgttc
     1981 gcgttgcgct ctttgacgat aaggcccaca gagatgccat tgcaaaacaa ctccaaaagc
     2041 tgaaaataag caaaatcctt attctaggca cttcagataa gatggtcaat aaaatagcaa
     2101 cacgattaca attgcctttg ccaactaaaa ttattcgcat tgaagatata gcaacgcaag
     2161 atgaaataga atacgcgatt cgctctcgac gtgttgaagg caaacatgtc attccggtac
     2221 ctgccataga agtgcgtaga aattatccac agatttttta caatgcggta aaggttttat
     2281 ttaagcgaaa aaaaactccg atcatctctg gaaacaatac aaaggtatat gaaaaatcag
     2341 ttgtgcgccc agagttttcc aaaaaaggac ggatagaaat atccgaagcg gcgctttcac
     2401 agatggttat gcactgcgta catgaatttg acgaagaagt gcgcttgaaa aaaatgtcga
     2461 ttaaaacaga tgaacgcgga taccgtttga ttattaccct cgatttaccc tttggctccc
     2521 agctaacggg taaaattcac agcatgcaaa aatacattat cgataatatc gagcaatata
     2581 cgggaatatt gattgaagag gtaagcatta tcatcgataa aattcgccgc acaaaaaaaa
     2641 gctagctatt ttctgttttt tttatccgca cttgtttttc ttttttaatt cgctttggct
     2701 ttttaactcg tataccatag gattgtagga ttttttttat agcaaattcc aattctatac
     2761 gttgtggatt tataggtaaa acaaagtgcc gcactcgagt ccatgttttt ccataaagct
     2821 ccgcaatatt tgcttcctca gcctcttttt cccagtcggt caaaccatca acaaagtctt
     2881 gcacgagaaa aataagtttt tcaccttgtc gtatattttg gttttggctg acaagtttgt
     2941 cgagcgcctg taagtgcgcc atataggatt gctcaaagtc ttttgactca gaaaggagcg
     3001 ctgtcgcttg cggttgcaaa tacatataga gcgatgtttc tagagacaag cttataattg
     3061 catgggcatg ccctgaattg agaatcttaa aaaattcttc ggtaagtctt gaaggtgata
     3121 taggcgataa aagatggctt gattttttta tctgtcgctt taaattatgt ggaagagaaa
     3181 aatttgtgag ggctgcatat tttaccgcgc gaatcatacg aacaggatct tcaacaaaga
     3241 ttttatcgat ctgtatgaca ggtcgtatgc gttttttttc tatatctttt acaccgttga
     3301 cataatcgat gagttgtttt tttacagggt cataatacag agcatttatg gtaaaatccc
     3361 gtcgcattac gtcctcgtcc attgtgccaa aatcattgcc tatggagcaa ccgtcgcaaa
     3421 tggatcgaaa ggtagaaact tcaaaaattt tggttccaaa gaaaatatgc acaatgcgaa
     3481 aacgtttacc gattatgcgt gagtttctaa agattttttt gagacgttgc ggagttgcat
     3541 cggttacaag gtcaaaatcc ttcgggacat ttccaagcaa gaggtcgcgc actgcaccac
     3601 cgacgataaa ggcttcaaaa ccatggtcct tgagcttttc gattacagct attgcgtcag
     3661 aatctataga agcatttgag atggggtgtt cattttctgt gtaaatgaca gcttttttta
     3721 caggattgcc attcttatcg ttcgtatagc ggaataacac agttccatca tatacaattg
     3781 gataaaaatc gtcaatcgct atcgtatagc tattttcgta aatctatttt attgataaaa
     3841 ctgagaatat ctttatagac cttttctttt tgtaattcat tcaggcactc atgtcgcgca
     3901 ccttcataga ctctaagttc aacgtctaaa ccacggcttt tgtaggcatc gtaaagccag
     3961 cgaaggccct ttgtataatt gctcacaggg tcatcacttc caacaaataa aaaaataggt
     4021 agattttttg gtatggcatc gagtttcgct tttttattta taatagtaaa gccatatcct
     4081 aaatcacgat aaaatccata ggtgcaatcc ataccgcaga aggggtctgc atcgtaggct
     4141 tctactgccc tatcgtcgct gctaagccaa gagtttttac tctttggatt tacaattctt
     4201 ttattgtagg atccaaaggc aatttttgtt aagagcggtg attggtggcg tattccccta
     4261 aagagggata aaaagcgtgc aaagagattt gaaaatcttc caagcggggc gttattggct
     4321 gttgttccag agagaataag agcatcgatg agttctgcat atgaagcaat aaaattttgt
     4381 gcgacaaagg agccaaatga atgcgcaaga agaatatagg gaacatcttt atgggtgttt
     4441 tttgtaaagt ttataatttc gcgaagatct tcggtaacac gattaaaacc gtctttgtct
     4501 gcaagatgcc ctgcaggggc tctctcttgt atactctgtc catgtccgcg atggtcgtgt
     4561 acatagaccg caataccgta attatttaaa tattgtgcaa aggcatcgta gcgtggtgca
     4621 tgttccgcca tcccatgact tatatgcaca acctttttta cttcgccacg tgggagccaa
     4681 gagtgcaagg caatgttcag ctcatccgac attctaactc tttgaatttc tcttttatca
     4741 gtcatttctt gtttcctcac tttttatttt ttatgcttct ctaaattttc atttacgttt
     4801 atagtataac acgtgaacaa aacactttgc aaacaatttt tcaccctaat tctctttttt
     4861 ccctccattg gctttgtttt ttcgcaatcc actgtccttg gcacgaaaag ttggcaaacg
     4921 gtgctttctg gaaacgtgct ttcaaaaata gaaccgagct cttacggctt tgttgtaatt
     4981 acggaaggga gattacttaa cgccttttct gaaaacggac agattctctg gcaaagaaac
     5041 cttggccaaa aaggcagtgg cttttgtgta agcatgaacg gcgattttgt gtatactgcg
     5101 attgacaaaa aactcagctt atttaataca agtggaattg ccctctggac tatagaaaca
     5161 aaaactccga ttgtagaaaa accgcttcct ggatgggacg ggcgtctctt tgtacgagaa
     5221 aaaaacgcta tttcaagcta cacaataaac ggactcttac gctttcgtct cgaagtaagc
     5281 gatgggcttg attttccctt ggtaagcctc aatgatggct cccttttata tgtacaaaaa
     5341 aagaacctga atgggaaatc gaccgcccta cgcatttcac cctttggagc tatcttagaa
     5401 gaaattattt ttgttgacac gataaggagt cttacagaaa gtaaaaatgg tcttgttttg
     5461 ggcttagaaa acggaaacct cgcagtctgc catgttgtag acaatctctt cgatacaaaa
     5521 tggtcaataa aaatagaaaa taaaaacata ctcgatgtat ttttctggga agacagtatt
     5581 ctcgtgcaat tttccgatgg aagttttgct ctttttgacg aagaaagcaa agaaaaagtt
     5641 tggcatacag gaagcattac aaaagaaaaa atagaagatg ctatttttga catggaaaag
     5701 ggtattttta gaagtttttc aaggattttt gtcataaata aagacggtag tgtcgaaaaa
     5761 aacatacaag ttcagaacaa ttctcgcttt tcgggactca cagagtctgg tttttttgta
     5821 gaagtaaagg atgattggtc tataagtgct taccggatcg cgcttgtaga aaatcgcctc
     5881 cataagcaaa gccagataaa aacatatcca cttcctcctg tgaaagcatc cagtgcagaa
     5941 ttgtataaaa atgccccagt cctcttgcaa agtataaatc tttttgaaac agaggctgaa
     6001 cttatctcac gccttcgact tgatataaag accattcaaa actcgtatct ttttatggaa
     6061 aaaccgagtc aaaattttga aacactcgcg ctaaaaataa aaacgattca aaatgcggcc
     6121 tacctcggaa cgggtgaacc gatgagcttt tttgaaagca tcttagacag ggaaagcgat
     6181 gaaagcattc tttttcaatg cttgcgcgcc atacaaaaaa atgcctacga ttatgatatg
     6241 cgcctccttc ttgcgattga aaaacgatta gtccagagaa atcagatttc agatgcgctt
     6301 ttagaagaat attgttatac cataacagaa atatgtcgct ttatgggaag aaaaaccttt
     6361 atgagaaagg gaaaagatct tctcttttct tttttagatg caaaatataa aatggctgtc
     6421 caagaaacag ctcgagaatg cttggaaaaa ataattgctt ttgaaaaata atagcaaaat
     6481 cctttgagct gtgctatact ctgtatatat tcgcatagga gttatatgat gataaaaaaa
     6541 agagccttta cgctactcat ccttattttt ttccttcccc tttttagttt agaagttgca
     6601 cgggatgaat tgcgcagcgt tacagaaaca atagagttta taaattatac gggaccccat
     6661 acagtcattc aaagtgtcga tgaaatacgc ggtattgggt taaggcttgc aactgcggta
     6721 aataaaaaca atctaatggc tccaggaaca agtggcagtc aaaatctcta ctatgtgata
     6781 catgcagtcg acccgaacac aaaagaaaaa ctagatgcag atattttaat cataggcgaa
     6841 aatgcacaag tcgatcacgt ccaaaaccta cggcgcattc tatcaagcta tctaagctca
     6901 gcctatgact atagtcgcca agatgcagat accctggcac attttattac catctataat
     6961 gctgtctatc gctcaaagat ggatgtgttt aatgctaagt acaaggccct tgtaacaggc
     7021 tatttacagc aagaaaaagt aggtctttct gtgaactacg tcgattggcc aggaaaaaca
     7081 caaatagtca ttccactttt agatttatca ggtggtttaa gtaccgttga tactacgatt
     7141 atttcagata aaagcgttgt cgattctatg cgtgaggcag acggaaaaga aatagatgtt
     7201 cgaaaagaca tggttgactt aaaagaacgt gaggcagatg cagcaagaga agaagcacaa
     7261 agctcacaaa aagaagcagt acgggctgaa gaaaaagcaa aagaagcgct tgtagaacaa
     7321 aaacaggaag agcaaaaact cgcagaaaaa aaagaagagg cagctaaggc cgtagaaata
     7381 gcgaaagaaa atcctgaaga tgtgcaagca caaaaagcgg ctgaagaagc agaaaaagaa
     7441 gtggttgccc aagagcaaaa actagcggaa gcaaaagaaa aaacagaaga ggctaaacaa
     7501 gaagctgagg aattaaaaac aattgcaaaa gaagaacaag ttatagcaga cagaaaaaca
     7561 gctgaggcgc aacaagatag actcgatatt gccaaggatc aaaaagaagt aatggcagca
     7621 gaagatgccc gagcacaaat gacgacaagc tatgcgctca aggttatcga tacaaaagca
     7681 cttttaagtg cccttgttct catcgatgtt gctgacggtg cggttgtaaa agaatccccg
     7741 gtcaatgtga ttcgaaatag gcaggttata aaaacaaacg aaggctatct cgctgtcgca
     7801 ggaaagcctg gaaaaaacac aacaattaag cttgttatac ttgatcccaa aaatttggaa
     7861 atacaaaagg aaagtgcaga aatagtacac gagtcctctg ccttcacctt tacttccaat
     7921 gctgtttttg ttgttatcgt tcaagataag aaaacctatc ttgcagccta caaccacgat
     7981 ttaagcctct ctgctcgctc agaagtagaa gtacaagatg tgagccccat tatcataaca
     8041 gatagaggca tatcggttat aaaggcagat ggcaagccca tactcctcga tgctaaaacc
     8101 ctcaaaaaac aatagtagca aaaaagtaaa aagaaaaaaa gcttagtcgc agatgactaa
     8161 gctttttttc ttttaccagc cataaacttc taggtagcgc tttgctgaag cgtcccagct
     8221 aaagtctttt ttcataccgc gcagtttcat tgcttctata tgtgcttttt tattgtacca
     8281 agcatagata gcccaaccga cagtgtcata gatacttccc ggtgtaagat tgtcgaaaac
     8341 aaaacccgtg ccttctcccg tctcttcgtt gtagttttca acagtatcgg ctaagccacc
     8401 agttcgtcgt acgatgggaa gtgttccata gagcaaggag tacatttgat ttaatccaca
     8461 gggctcatag cgtgagggca tgagaaaaaa atcacttcct gcttctatga gatggctcaa
     8521 gccttcgtcg tagccgatat aggcacgaaa attgtcgagt tttccttgca aacttcgcag
     8581 ctcatcctca caccaagact cgcctgagcc caagactatc atttgcagct tcatatcgcg
     8641 acagatgctg taggcagaac caaaggtcgg tgcaaaaagc tcagcaacac ccttttggtc
     8701 ggcgagcctg gtcacaattc ccacgatggg aatatcggga tttacctcaa gccccattcg
     8761 ctcctgcaag gctcttttat tttccgcctt tttggcaagc gttttcgtat cataatttac
     8821 cggaagatac ctatcctttt tgggattcca ataatccata tctacaccgt tgagaatacc
     8881 ggtaagagaa taggagcgtt cacgcaaaag cccatctagg ttaaagccat aggcagctgt
     8941 ttgaatttct tgggcatagg taggagaaac cgtcgttagg tggtcagcgc tccgtatccc
     9001 agctttgaga aagttaatac ggtcaaagtc ctcaaaaccc gagtcataaa aatgcgacca
     9061 gtcaagccca aaaagcggat aatgatgctt gggatataca ccttggtagc cgaggttgtg
     9121 aatcgtcaaa accgattttg tctgtgcaaa aacaccatga cgcatattga actttaaaag
     9181 agctggcaca agacttgccg accagtcgtg agcgtgcata acatccggat accagcctaa
     9241 tttatgacat aattggaagg aagcatgtcc caaaagagaa aaacgccgtg gattatcgtg
     9301 aaaatctgac tcaaagggag taccgtagac accgtcacgc ccaaaacatt gttcatggtc
     9361 tataaaataa ataggaagac ttggatagtc tttatgccca gttggaatat ctgttttata
     9421 aaccgctgtc cactcctctt tataacttag atgaaccgcc atcggctcat ctaaaagctc
     9481 gagtttgtcg cgatctattt tgtagtagcg aggcataata atgcgcacat cagcgcccgt
     9541 ttttctcagt gcaatcgcaa gtgccgttac cgcatcagcg agaccacccg tctttgcaaa
     9601 aggaacagct tcggcagtta tcattaaaat tttcattatt ctattgtagc agactttatg
     9661 gaattgtcca gttttaaaaa acaaaaatct ctgcttgtga ataagcagaa aagatgttaa
     9721 aatgcaatca aatgactcat gacgaattgt taattagaat acaaaatggt gaagatagtt
     9781 ttactcaatt taaaagacag gcaattcctg caaaagagct ggctaaagag tttgttgcct
     9841 ttctaaatgc cgaaggtggt attattgttt ttggtgttga agataaaaca aacgctattc
     9901 ttggtttaac acaaatagaa gtagagcaaa taggtcaact gataggaaac tctgcaaatg
     9961 aaaatgtaaa gccgcccttt catcctatag tcgaaaatat aatgattcag gggaaacgac
    10021 ttgttattgc ctatgttccc aaaggtataa gtaaacccta tgcaacaaat aatggggagt
    10081 tttttataaa atcaagctcc gataaaaaga aaatttctca agatgagtta cgaaggctat
    10141 ttgcagaatc aaagcgccta tttgctgatg aggaaattgt acatgggacg agcataaaag
    10201 atttagacac tacttctttt gctcgttttt tagaaaaaga taatccctat atttatggtc
    10261 aattacaaga agatgtgcta agcctgcaac aaataatgat aaacttagat cttttaaaag
    10321 aagagcaact aacacttgct ggaaatcttt tgtttgggat acacccacaa aaatataata
    10381 aatcatttta tattgattgt tgtcattttg atggtattga ttcttctgtt gatacttata
    10441 agtctaaaag aaaaatggac ggtccttttc atacgctttt ttttaattct ctggagtttc
    10501 taaaaagtag tttacgaaac atgcaaacga atgctgattt taacagtagc cctcagctag
    10561 aaattgatga gcgtgtttta agcgaactta tagtaaatgc cttggtacat agagattatt
    10621 atattttgtc atccataaaa atatttattt ttaccgatag agtcgaaatt atcagccctg
    10681 ggaaactgac caattcatta actgttgata aaattaaaag tggtttgtca attcacagaa
    10741 accctatttt aaattctatc gcaaaaacaa ttcttcccta cagcggctat ggaagtggta
    10801 taaaaagatc tctgaatatt ttaccaagta tagactttat aaatgatata gaaaaagaac
    10861 aattcatctg cataattcct cgtcctagat aaagtctacc agctttcacg cgctttctta
    10921 aacgcttctg ctgcattcat aatcgttttt tctggaacgc agtaggaaat gcgaaaataa
    10981 ccaggac
//
LOCUS       DAOSSW010000059        10709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_6032_length_10709_cov_6.389150, whole genome shotgun sequence.
ACCESSION   DAOSSW010000059 DAOSSW010000000
VERSION     DAOSSW010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 10709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10709
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6032_length_10709_cov_6.389150"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /gene="lptB"
                     /locus_tag="PKW26_05945"
     CDS             <1..543
                     /gene="lptB"
                     /locus_tag="PKW26_05945"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOQ93144.1"
                     /translation="LNEQCITKLPMYKRARLGISYLPQEPSVFRKLSVEKNIWAILES
                     RQDLSHTEKKEHLENLLEEFNIAHLRKQSAYTLSGGERRRTEIARSLAIEPKFLLLDE
                     PFAGIDPIAVADIKEMIQILANRGIGILITDHNVRDTLEITTRSIIISEGQIVSQGNK
                     EEILESPLARKLYLGESFKM"
     gene            678..2207
                     /gene="rny"
                     /locus_tag="PKW26_05950"
     CDS             678..2207
                     /gene="rny"
                     /locus_tag="PKW26_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOQ93145.1"
                     /translation="MKYVLFIGLPVAGVVLGWTIRWLYARIQLSASEQRAERITQDAI
                     KEAEAQKKEILLEAKDQLIRERNQQERENRERRGELQRVERRLLLKEETLDKKENTLN
                     DQEKEFEKREKHIVAKEEELSLQETRYIQELEKISGFTADEAKAIIIQSLENEAKHDA
                     QALLNKIEQEAQIASEKKAREILVATIQRIATETTSDVTVATVSLPSDEMKGRIIGRE
                     GRNIRTLETLTGVDVIIDDTPEAVVISCFDPVRKEIAKVALERLITDGRIHPARIEEI
                     VQKVTKEIQHKIYEEGEKVLFDLGIHNMSQEGVRALGRLYFRTSYGQNVLMHAKEVAI
                     IAGMLAGEIGANKELAKRAAVLHDIGKGAETDSDQNHAEVGMDLARKMGEDPRVINAI
                     GAHHNDVEPTTIEAIVVQIADAISAARPGARRETIDNYVKRLENLEAIAEHFEGVEKA
                     YAIQAGRELRILVNNERIPDAEVKDLARNIAKQIEADLKYPGRIKITMIRETRIVEYA
                     R"
     gene            complement(2292..>3087)
                     /locus_tag="PKW26_05955"
     CDS             complement(2292..>3087)
                     /locus_tag="PKW26_05955"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOQ93146.1"
                     /translation="FGRSFSLDAVFGDLAMKISPLFSKKNTEVFIPACFDAFIGADLL
                     AASLGTGLWQLEEKASHFDANSAETSQLLIDIGTNTELVLRHRGKNLVASTAAGPAFE
                     GGKTKSELKGSELLHALSLMLSQGQMDATGFILVEKPLLCQEDVRVLQLAKAAIRAAV
                     DTLLFEMQIDAEEIDAVFIAGAFGSHIEVKDIEKTGLLDGVLAKKISLRENAVLDGAF
                     LYTKAALEADFHAVSSLVEKEFSQTTLVQLADNAFFAKAYIEAMSF"
     assembly_gap    3088..3187
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3188..3550)
                     /locus_tag="PKW26_05960"
     CDS             complement(<3188..3550)
                     /locus_tag="PKW26_05960"
                     /inference="COORDINATES: protein motif:HMM:NF036965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93147.1"
                     /translation="MNLVIDIGTTNIIFFDGQRKAVFRNPQYRFGSDIMSRILHAMQK
                     GQEIQTQLLREALAEGFFSFLNDKKIQSLVIVANTVQLHFLLGLDISGMATFPFTPLE
                     FFGRSFSLDAVFGDLAMKI"
     gene            complement(3580..4176)
                     /gene="pyrE"
                     /locus_tag="PKW26_05965"
     CDS             complement(3580..4176)
                     /gene="pyrE"
                     /locus_tag="PKW26_05965"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOQ93148.1"
                     /translation="MDIISILQESEAMLSGHFLLSSGCHSDKYFQCAKLLQYPDKASL
                     VLSVPVQEILAEKNAEKIDFDAVVGPAMGGIIPAYEFARQLGIPAFFTERNEDGDMVL
                     RRGFEIKEGMKILLAEDVITTGRSTLESAKVLQDLGAKIVASICIVDRRDENAENPFS
                     WPIFSALRSPTLNFEPTNCPLCDAGEIPLVKPGSRKKI"
     gene            complement(4257..5276)
                     /locus_tag="PKW26_05970"
     CDS             complement(4257..5276)
                     /locus_tag="PKW26_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOQ93149.1"
                     /translation="MDLSLKPISGRAFLAYPEMLDFLLLEIRERFFIDVKNIKNSDSD
                     YSEKEISIFPDSSEPLLFGNLLYIPQWKEKKNPYWAATVLEAPALLDFSSIKQAALSL
                     RNIQRNWTSYQYKLFRRAALIVESLPYINPKPRSFPYLIPESPMGLFTLVKKELILCS
                     AKTSSPLAAGCLTFVEDKIEPPSRAYLKFQEALTRLYSAKGSIPQKNERCLDLGACPG
                     GWTWVLRQLGCEVLAVDRTALDEKLMKDPKVRFIKHDAFTLPLEELGPFDWIFSDVIC
                     YPERLLEWVKKILASGITKKMICTIKLQGDIDWQIIDDFAEIPHSFVQHLCYNKHELT
                     WFYCE"
     gene            complement(5401..5823)
                     /locus_tag="PKW26_05975"
     CDS             complement(5401..5823)
                     /locus_tag="PKW26_05975"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOQ93150.1"
                     /translation="MSVFLVLFKLDNLHKTSDSISMGKADLRKAKRFEEVAKVSVPNL
                     SQFSGVLVDVSKIGCRIHFNVFLNIEMDMEYEILIFPVLADIDSFTLVGEPVWVQNKS
                     KSTEIGFKFLRSVGTKPFEAYIDNLEDREKKTFWEERL"
     gene            5881..6942
                     /locus_tag="PKW26_05980"
     CDS             5881..6942
                     /locus_tag="PKW26_05980"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOQ93151.1"
                     /translation="MNDKTISVAVFDAKPYDIVFFNKANEKYGYDLHFFEFRLKASTA
                     HAAKGFDVVCAFVNDAIDEKTVNILKEHNVQLITMRCAGYNNLDLKALFNTIHVTRVP
                     AYSPYSVAEHAVSLLLALTRKIPQAFNRTKSGNFSLVGLTGRDMQGRTIGIVGTGKIG
                     KIMANIAKGFGMNILLFDVFPDQAWAKNIGAEYVSKERLFKESDVITLHCPLTPETRH
                     FVDLPSIELMKNDAVIINTGRGALIDTKALVHALKHKLIGGAALDVYEEEDKYFFEDW
                     STEVIEDDTLARLMTFPNVIITSHQAFLTQDALESIAHTSFENIRAYFAGEALENEIC
                     YQCGQNRDSCTHAKTGRCF"
     gene            complement(6981..7063)
                     /locus_tag="PKW26_05985"
     tRNA            complement(6981..7063)
                     /locus_tag="PKW26_05985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7028..7030),aa:Leu,seq:cag)
     gene            complement(7140..8063)
                     /locus_tag="PKW26_05990"
     CDS             complement(7140..8063)
                     /locus_tag="PKW26_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOQ93152.1"
                     /translation="MNKHKITMLIIAFFWLLLAIFASLFFIRLLTGRSCPLSLKSRFS
                     GNTKNLSVEEYQFDVEDISELSLELISESIDLTKSSDNFIHVRVSSLAGSESPVPRIK
                     NHVLEIKRNKPKTRFFSFYAFKESVEVAIPESLMLKKDFLIQAFSTSGSISMSDMSAS
                     EIFAYSTSGSIRAKNVNAKKFDFKSTSGSVKVEDSSCKNAALHSTSGSIVFEGLADEA
                     DLRSTSGSVNAVFLEMPKKDSRFVSTSGAVRLSLPENDGFDFHYSTISGSVRNEFTGF
                     RGKKSGVNRYKDGGIAFDLTSTSGSVRIERN"
     gene            complement(8077..8769)
                     /locus_tag="PKW26_05995"
     CDS             complement(8077..8769)
                     /locus_tag="PKW26_05995"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HOQ93153.1"
                     /translation="MSTKIRNYVEVLFSDIPRTKKSQELKEEILANVTERFNAYIAQG
                     KTENQAYSLAVSEMGDIDEMLQSIAPDRELKPKIDAYRVKRAKNTAIAVSLYIIGVAF
                     LILFGGLPTMLDMDILEDTGGIIGLIVLLVFSAIATAMLVYTYLAVPQDIEPYLRERQ
                     KDDYDLGRVSEQKRMLFKSVSSLLWLAITIIYLLVSFTTGAWHISWIIFLIGSFVQQS
                     IRTFMMIDSDKE"
     gene            complement(8753..9118)
                     /locus_tag="PKW26_06000"
     CDS             complement(8753..9118)
                     /locus_tag="PKW26_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOQ93154.1"
                     /translation="MLQYSDILRGLTDSLILAQLEYGDSYGYEISKTILKESDGSIEL
                     KEASLYTSFRRLEEAGYITSYWGDENSGARRRYYTLNDEGKVFITENKEDWKKISKQI
                     SDLLSGKKKKEKNKDEYKN"
     gene            complement(9280..10617)
                     /locus_tag="PKW26_06005"
     CDS             complement(9280..10617)
                     /locus_tag="PKW26_06005"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOQ93155.1"
                     /translation="MKIKYFIFMVIFFIVFNSCNLESNLVVENFQKKEKAWIFLVYMA
                     ADNDLESAAIRDFNELEAAQFDRAKISILVLLDRSPFYDNSNGDWSGTRLYEISYDPN
                     GIDNIIRSKEIVFPLLDIGGGKQTNVNTADPFVLEGTIDFVKQKYQAENYGLIMWGHG
                     TGWRSPAQNNYRSASNESLGTKAFAFDDTSQTYMSLSSFHKAIAGKGLKCIGFDTCFG
                     AVLELAYEICDETEWIIASPSTVPFEGWDYKNLFNFFTKTDFSVESFSISILEQFQLQ
                     YAQENTAALSIIKTDKIAALKESFDSFAKKLATCIVDTEERHSCLGWILEEVLTYKDT
                     SARTDVFVDIASFVEEGGKRYQDLMPQAEIVLLKLSEVLPLFYSTEKRDYGGLGVFFI
                     GLENGIPNASHPNDYVQGSDFGECSRFVKESDGWVPHKNGGDSLLDVLFYTTF"
BASE COUNT         3346 a         2186 c         1964 g         3113 t
ORIGIN      
        1 ttaaatgagc aatgcattac aaaacttccc atgtataagc gcgcgcgttt aggtatctcc
       61 tatcttccac aggaaccttc tgtttttcga aaactcagcg ttgaaaaaaa tatctgggct
      121 attttagaat caaggcagga tttaagtcat actgaaaaaa aagagcattt agaaaacctc
      181 cttgaagaat tcaatatagc ccatttacga aaacaatcag cctacacact ttctggtggt
      241 gaacggcgac gcacagaaat agctcgatct cttgccattg agccaaaatt tttgctcctc
      301 gacgaaccct ttgccggcat agaccccata gcggtagccg acataaaaga aatgatacaa
      361 atcctcgcaa atcggggcat tggtatcctt atcaccgacc acaatgtacg cgacaccctc
      421 gaaataacca cccgttccat cataataagc gaaggacaga ttgtaagcca aggaaacaag
      481 gaggaaattc ttgaatcccc ccttgcgcga aaactctatc tcggagaaag ctttaaaatg
      541 tagctttgta gccacttttt acatccttaa aagccaccaa tcttccccct ttctatacgc
      601 ttttatgtag ttatgtttga caactcacct cttttgtgat attctgtacc ttgaacttgt
      661 ctagtttgga ggacctgatg aagtatgtac tatttattgg tctcccagtt gctggagttg
      721 ttcttggatg gactattcgc tggctctatg ccagaattca attatctgcg tctgaacaaa
      781 gagcagaacg aattacgcaa gatgcgataa aagaggctga agctcaaaaa aaagaaattt
      841 tattagaagc gaaagatcaa ctcattcgag agcgaaatca acaagagagg gagaacagag
      901 agcgccgggg cgaacttcaa cgagtagaaa gacgattatt gcttaaagaa gaaacacttg
      961 ataaaaaaga aaacacatta aatgatcaag aaaaggaatt tgaaaaaaga gaaaagcaca
     1021 ttgtagctaa agaagaagaa ttatctttac aagagacacg ctatattcaa gaactcgaaa
     1081 aaatttcagg ttttactgct gatgaagcaa aggcaattat cattcaaagc ttggagaacg
     1141 aagcaaagca tgatgcacaa gccttgttaa ataaaattga acaagaggca caaattgcat
     1201 cagaaaaaaa agcacgcgaa atcttagttg caactataca acgtattgca actgaaacaa
     1261 cgagcgatgt aactgttgcg acagtttcac tgccgagcga cgaaatgaag gggcgcatta
     1321 ttgggaggga gggaagaaat atccgcaccc tcgaaacact tactggggtc gatgttatta
     1381 tcgatgatac accagaagcg gttgttattt cttgttttga tcctgttcgc aaggaaattg
     1441 cgaaggttgc acttgaacgg ctcattaccg atggacgcat tcatccagct cggattgaag
     1501 aaatagtgca aaaggtaaca aaagagattc aacataagat ctacgaagaa ggcgaaaaag
     1561 tgctcttcga tcttggtatt cataatatga gccaagaagg agtgcgagcc cttggtagat
     1621 tatactttag aacgagctat ggacaaaatg tccttatgca cgcaaaagaa gtagcaatta
     1681 ttgcaggaat gttagctgga gaaattgggg caaacaagga acttgcaaaa cgagctgcgg
     1741 tattacacga tattgggaag ggtgcagaaa ctgattcaga ccaaaaccac gcagaagtgg
     1801 gaatggacct tgcgcgaaaa atgggcgaag atccaagggt tataaacgca atcggtgccc
     1861 accacaatga tgtggagccg acaacaattg aagcaattgt cgtacaaatt gcagatgcaa
     1921 tttctgctgc acgtcctggg gcaagacgag aaaccataga caactatgta aaacgtcttg
     1981 aaaaccttga agcaattgcg gaacactttg agggcgtaga aaaagcctac gcaattcaag
     2041 caggacgaga actccgaatt ttggttaaca atgaaagaat tcctgatgca gaggttaaag
     2101 atttggcacg aaatattgca aaacaaatcg aagctgacct caagtatccg gggcgaatta
     2161 agatcacgat gattcgtgaa acacgtattg tagaatacgc tcgctaagaa aaccaaaatc
     2221 tacaaaaacc ttcaaagtct cgcgctttga aggttttttt tatcttttac aatctatgta
     2281 taaaacgata cttaaaaaga cattgcctct atataagcct ttgcaaaaaa agcattatca
     2341 gcgagctgaa cgagggtcgt ttgagaaaat tctttttcta cgagacttga tacggcatgg
     2401 aaatctgctt cgagagcagc ctttgtatat aaaaaagcac cgtctaaaac cgcattttcc
     2461 cgcaagctta ttttttttgc caaaacgcca tccaaaagtc ctgttttttc tatatccttc
     2521 acttctatat gcgagccaaa ggctcctgca ataaaaacgg catctatttc ttctgcatct
     2581 atctgcattt caaaaagaag ggtgtcgaca gcggcacgga tagcagcctt ggcaagttgt
     2641 aaaacgcgaa catcttcttg acataaaagc ggtttttcaa ccaaaataaa gcctgttgca
     2701 tccatttgtc cttgactcag catgagggag agcgcatgaa ggagctcgga gcccttgagt
     2761 tcgctctttg ttttgccgcc ttcaaaggct ggtcctgcag cagtcgatgc cacaagattt
     2821 tttcccctgt gccgcaaaac taactcggta tttgttccaa tatctatgag caattgagaa
     2881 gtttctgcag aatttgcatc aaaatgacta gccttttctt ccagctgcca aagccctgta
     2941 cccaaactag ccgcaagcaa atctgcaccg ataaaggcat caaaacaggc gggaataaaa
     3001 acctctgtgt tttttttgct aaaaaggggc gagattttca tagcaagatc gccaaaaact
     3061 gcgtccaagg aaaaagacct tccaaaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnngat tttcatagca agatcgccaa aaactgcgtc caaggaaaaa gaccttccaa
     3241 aaaattcgag cggtgtaaaa ggaaatgttg ccataccgct tatatctaag ccaagtaaaa
     3301 aatgtaattg cacggtattt gcgacaatta caaggctttg tattttttta tcatttagaa
     3361 aggaaaaaaa accttctgca agagcctcac gtaaaagctg cgtttgtatt tcttggcctt
     3421 tttgcattgc atggaggata cggctcataa tatcgctgcc aaatcgatat tgggggttac
     3481 gaaatacggc ttttctctgt ccatcaaaga aaataatatt tgttgtgcca atatcgatta
     3541 ctaaattcat ttttttctcg agcttttgcc tctatccgac tagattttct ttcgtgagcc
     3601 tggcttaacg agcggtattt ctcctgcatc gcaaaggggg caattagttg gctcaaaatt
     3661 gagcgttggt gagcgcaatg cggaaaaaat aggccagcta aaggggtttt ctgcattttc
     3721 atctctacgg tcgacgatgc aaatagatgc tacaattttt gctcctaaat cttgtaaaac
     3781 ctttgcactt tcaagcgttg aacgcccggt ggtaataaca tcttctgcaa gaaggatttt
     3841 cattccctct tttatttcaa aaccacgacg caaaaccatg tctccgtctt catttctttc
     3901 ggtaaaaaat gcagggatcc ccagttgccg tgcaaactcg taggcaggaa taatgccacc
     3961 cattgcagga ccgactacgg catcgaagtc tattttttct gcgttttttt ctgcaagaat
     4021 ctcttgcact ggcaccgata aaacaagact tgctttatcg ggatattgca ataattttgc
     4081 acactgaaag tatttgtcag agtgacagcc tgatgataat aaaaaatgcc cactgagcat
     4141 tgcttcactt tcttgtaaaa ttgaaataat atccatttcc tacctcgttt tataaaaaca
     4201 tactatcctt aatttttatt ttaggaagtt atctatgagc atagcaaatt ttttgtttat
     4261 tcgcaataaa accaggttaa ttcgtgttta ttatagcaaa gatgttgtac aaaactatgt
     4321 gggatttcag caaagtcatc gataatctgc cagtctatat ctccttgtag tttaattgtg
     4381 caaatcattt tttttgttat gccagacgcg agtatttttt ttacccattc gagtaaacgt
     4441 tccgggtagc aaatgacatc ggaaaatatc caatcgaagg gtcctagttc ttcaagcggc
     4501 agggtgaaag cgtcgtgttt gataaagcga accttggggt ctttcataag cttctcgtca
     4561 agagcagtcc tatcaaccgc aagaacctcg cagcctagtt ggcgcaggac ccaagtccag
     4621 cctccagggc aagcaccaag atcgagacag cgctcatttt tttgcggaat gcttcctttt
     4681 gcactgtata aacgcgttaa agcttcttga aactttagat aagctctgct cggtggttct
     4741 atcttatctt caacaaaagt cagacaacca gcggctagag ggctgcttgt ttttgcactg
     4801 cataaaatga gctctttttt cacaagcgta aataaaccca tgggactttc gggtatgaga
     4861 tagggaaaac tacgcggttt gggatttatg tagggaagcg attcaacgat gagggcagcc
     4921 cgacggaata gcttgtactg atacgaggtc cagtttcttt gaatatttcg taaagagagg
     4981 gctgcttgct ttatggaaga aaaatccaag agagcgggtg cctctaaaac tgtcgctgcc
     5041 caatagggat tttttttctc tttccactgg ggaatataca agagattccc aaaaagaagg
     5101 ggctcgcttg aatctggaaa aatacttatt tctttttcgc tataatcgga gtcagagttt
     5161 tttatattct ttacatcgat aaaaaaacgc tctcttatct ccaataataa aaaatcaagc
     5221 atctcagggt aggctaaaaa agcccgcccc gaaatgggtt ttaagctaag gtccatgaga
     5281 ccgagtatat caataaaaac gagtatgttc tatcgatttt ttatcagatt tttattcatt
     5341 ttctagcgat gtattttcgg gcctgttttc ctagcaagga agaagcatct tctaaaaaat
     5401 ctagagtctt tcttcccaga aagttttttt ttctcgatct tcaagattat cgatataggc
     5461 ttcaaatggc ttcgtaccta ctgaacggag aaatttaaaa ccaatttctg ttgattttga
     5521 tttattttga acccagactg gttctcccac aagcgtaaaa gagtcgatat ccgccagtac
     5581 cggaaaaatt aaaatctcgt attccatatc catctcaata tttagaaata cattaaaatg
     5641 aatcctgcaa cctattttac ttacgtcaac taagacacca gaaaattggc tgagatttgg
     5701 aacgcttact tttgcaactt cttcaaaacg ctttgctttt cttaaatcag ctttacccat
     5761 gctaatagaa tcacttgttt tatgcaaatt gtcaagttta aaaagcacta agaaaacact
     5821 cataacctat tgacttacta ggttattatt tgctagacta aatcatattg gaggtacaat
     5881 atgaacgata aaacaatttc agtagcggtt tttgacgcaa aaccctacga tatagtattt
     5941 tttaataagg caaatgaaaa atatggctac gacttgcatt tttttgagtt tcgcctaaaa
     6001 gcgtcgacag cacatgcagc gaagggcttt gacgttgttt gtgcctttgt aaatgatgcg
     6061 attgatgaaa aaaccgtgaa cattttaaaa gagcacaatg tgcaacttat taccatgcgc
     6121 tgtgcaggct ataataatct agacttaaaa gcgcttttca atacaattca tgttacccga
     6181 gtacctgcct attcacccta ctctgttgct gaacatgcag tttctctctt acttgcgctt
     6241 acaagaaaaa ttcctcaagc tttcaataga acaaaaagcg gaaactttag cctcgtgggt
     6301 ttaaccggaa gagatatgca gggtcgaaca atcggtattg tagggacggg aaaaataggg
     6361 aaaattatgg cgaacattgc aaaaggcttt gggatgaata ttctcctttt cgacgtcttc
     6421 cccgatcaag cttgggcaaa aaacattggt gcagaatacg tttcaaaaga acgccttttt
     6481 aaagaaagcg atgttatcac cctgcactgc cctcttacac ctgaaactcg ccattttgta
     6541 gatcttccgt ctattgagct gatgaaaaat gatgctgtca taataaatac aggacgtggt
     6601 gccttaattg atacaaaggc ccttgtacat gcgctcaaac acaagctcat cggcggagct
     6661 gcccttgatg tctatgagga agaagataaa tacttttttg aagattggtc aaccgaagtt
     6721 atcgaagatg ataccctcgc tcgccttatg accttcccaa atgtgattat taccagtcat
     6781 caagcttttt taacacaaga cgcgctcgag tcaattgcgc acacaagctt tgaaaatatt
     6841 cgtgcctact ttgcggggga agccttggag aatgaaatat gctatcaatg tggacaaaac
     6901 agagattctt gcacgcacgc aaaaacgggc agatgttttt aaaatacaaa agagctaggg
     6961 caaaatccca gctctttttt tccccggaag aagacttgaa cttccacgcc cttacgagca
     7021 ctcgcacctg aaacgagcct gtctaccatt ccagcacccg gggaaacatt atactattct
     7081 atcacttttt ttattctggt gctagaatcc aagatagatt tatgaatgtt tttctctccc
     7141 tagtttctct ctattctaac ggatccagaa gtagaggtca aatcaaaagc tattccacca
     7201 tccttatatc gatttacacc actttttttc ccgcgaaatc ctgtaaactc gtttcgaaca
     7261 gacccagaaa ttgtcgaata atgaaaatca aagccatcgt tttcagggag gcttaggcga
     7321 acggctccag aagttgagac aaatcgagaa tcttttttgg gcatctctaa aaagacagca
     7381 tttactgagc ccgaagtact tcttaaatct gcctcatcag cgagtccctc gaagacaatg
     7441 ctaccactcg tagaatgaag cgccgcattt ttacaagaag aatcttcaac tttgacactt
     7501 ccactcgtgc ttttaaaatc aaattttttt gcatttacat tttttgctct tatactgcca
     7561 cttgtagaat atgcaaatat ttctgatgca ctcatatcgc tcatgctaat gctgccgctc
     7621 gttgaaaaag cttgaattaa aaaatctttt tttaacatca atgattctgg aatagctacc
     7681 tcaacagatt ctttaaaagc gtaaaaacta aaaaaccgcg tttttggttt atttctttta
     7741 atttccaaaa catgattttt tattcttgga acagggcttt cggatccagc gagagaagat
     7801 acacgtacat gaataaaatt gtctgaagat tttgtaagat caatactctc agaaataagt
     7861 tcaagagata attctgaaat atcttcaaca tcaaattgat attcttctac actaagattt
     7921 tttgtatttc ctgaaaagcg agatttcaaa gacagagggc aggacctgcc tgtcaaaagc
     7981 cgtataaaaa agagagatgc aaaaattgca aggagaagcc aaaaaaaggc tatgattaac
     8041 attgttattt tatgtttatt cattaaaaca ctccatctat tctttgtctg aatcgatcat
     8101 cataaaggtg cgaatactct gttgtacaaa agaaccaatt aagaaaataa tccaagaaat
     8161 atgccaagct ccagttgtaa agctaacaag caaataaata atagtaattg caagccacaa
     8221 taaacttgac actgacttaa agagcattct tttttgctct gacacccttc ccaaatcata
     8281 gtcatccttt tgtctttccc gtagataggg ttctatatcc tgtggcacag ctaaataagt
     8341 atacacaagc atagcggttg caatggctga aaaaacaagc aatacaatga gcccaattat
     8401 tccaccggta tcttctaata tatccatatc gagcatagta ggaagaccac cgaataaaat
     8461 taaaaaagcc accccgataa tatataaact aacggcaata gcggtatttt ttgcgcgctt
     8521 tactcgatag gcgtcaattt ttggtttgag ctcgcgatca ggtgcaatcg attgaagcat
     8581 ttcgtcgata tcgcccattt cggaaacagc taagctataa gcttgatttt cagttttacc
     8641 ttgcgcaata taggcattga aacgctctgt tacatttgca agaatttctt cttttaattc
     8701 ttggctcttt tttgttcgag gtatgtcgct aaacaatacc tcaacataat ttctaatttt
     8761 tgtactcatc tttgtttttc tccttttttt tcttccccga taataaatcg cttatttgct
     8821 ttgaaatttt tttccagtct tctttatttt ccgtaatgaa aacctttccc tcatcattga
     8881 gtgtataata tcgtcttcgt gctccagaat tttcatcccc ccagtacgaa gtgatatagc
     8941 ctgcttcttc taaacgtcta aaagaggtat acaagcttgc ttcctttagc tcgattgaac
     9001 catcagattc tttgagaatt gtcttgctaa tttcatagcc atagctgtcc ccatattcta
     9061 gttgagctaa gataagtgag tcagttaaac ctcgcaatat atctgaatat tgtaacatat
     9121 atctcctttc aagatacata ttacaacaca atacatcgcc tatcaaggta tcttggtata
     9181 aaaaaagaaa ctatctcgtt ttggcggagt aatttataca gtaaaccaaa acgagtgtac
     9241 ttagtccctg cgacatccat gccgcatacg ttttttcatt taaaatgttg tataaaataa
     9301 aacatcgagt aagctatctc cgccattttt gtggggaacc caaccgtcgc tctcttttac
     9361 aaaacggcta cattcgccaa aatcactacc ctgcacatag tcatttggat ggcttgcatt
     9421 tgggattcca ttttccaagc caataaaaaa aacacccaag cccccatagt ctcttttttc
     9481 agtcgaataa aacagaggta gtacttctga tagtttcagc aaaactattt cagcctgcgg
     9541 cattaaatct tgatagcgtt ttccaccttc ctcaacaaag gatgcaatat ccacaaagac
     9601 gtctgttcgg gcactcgtat ctttatatgt tagaacctct tccaatatcc atccaagaca
     9661 gctatgtctc tcttcagtgt cgacgataca cgttgccagt ttttttgcaa aggaatcaaa
     9721 actttctttt aacgctgcaa ttttatcagt tttgataata cttaatgcag cagtattttc
     9781 ttgtgcgtat tgcaattgaa attgttcaag aatagatatg gaaaatgatt caacactgaa
     9841 atctgtcttt gtgaaaaaat tgaacaggtt tttataatcc cagccttcaa acggcaccgt
     9901 agacggagat gcgatgatcc attctgtttc atcgcagatt tcataggcaa gttctaaaac
     9961 tgcaccaaaa caggtatcaa agccaataca ttttaagccc ttgcccgcta ttgccttatg
    10021 aaagctagaa aggctcatat atgtttgact tgtgtcatca aaggcaaagg ccttagtacc
    10081 gagggattca ttagatgctg accgataatt attttgtgcc gggctgcgcc aaccagtgcc
    10141 atgtccccac attataagcc catagttttc cgcttggtat ttttgcttta caaaatctat
    10201 cgttccttct aagacaaaag ggtcggcggt atttacattg gtttgttttc ctcctccaat
    10261 atccaagagc ggaaagacta tttctttcga ccgaattata ttgtcgatgc catttggatc
    10321 ataagaaatc tcatacaatc gtgttcccga ccaatctcca tttgaattat cataaaaggg
    10381 acttctatca agtaaaacaa gaatacttat ttttgctcgg tcaaattgtg ccgcttctag
    10441 ttcattaaaa tctcggattg ctgcagattc aaggtcattg tcagctgcca tgtaaacaag
    10501 gaatatccat gccttttctt ttttttgaaa attttcaaca actagatttg attctaaatt
    10561 gcaagaatta aatactataa aaaatataac cataaaaata aaatatttta ttttcatccc
    10621 actacctcct actcaagact ataagcatag ctataaaata tatctttcca aacatctcca
    10681 tttgaaaaca ctacttcttt aatacaaaa
//
LOCUS       DAOSSW010000060        10528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_6160_length_10528_cov_6.349852, whole genome shotgun sequence.
ACCESSION   DAOSSW010000060 DAOSSW010000000
VERSION     DAOSSW010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 10528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10528
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6160_length_10528_cov_6.349852"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PKW26_06010"
     CDS             <1..547
                     /locus_tag="PKW26_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93156.1"
                     /translation="VIWFGFSQKGKTLGLSLTGSTLSGVAWGDFMREAHEGYPYKAFS
                     EPQTGLVKATVCSVSGLLLTEACGKSATTQYFLEGTQPLTMCDYHINRETAREIGALR
                     LLDARYQSGAQEAQIQDTGGLVLDLSFLNIKTSEDFDTYEKEKDARSESDAIYNDFFF
                     TIDDQDEDSDEDDEMSNFLLD"
     gene            581..1192
                     /locus_tag="PKW26_06015"
     CDS             581..1192
                     /locus_tag="PKW26_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOQ93157.1"
                     /translation="MMIVAISDIKIKKRIRKDLGDIDALMESLEQYGLLNPITVNTKN
                     ELIAGHRRLEAAKRLGWQNIHAVQIDIRDPLLLLELEMEENMQRLAFSDAEAQDGYAK
                     LEKLRNPHFIKKWWRNIAGFFSDLFSSNAKERLGKKVFQARKYLWLFPIGIFMLIVSG
                     FWNKTRLSGSIFKTLFDLSSLIILLFACFSLVRLIYLKRINEK"
     gene            complement(1179..2414)
                     /gene="tyrS"
                     /locus_tag="PKW26_06020"
     CDS             complement(1179..2414)
                     /gene="tyrS"
                     /locus_tag="PKW26_06020"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOQ93158.1"
                     /translation="MNEALQTLMDRGFFQQCTDLEGLSKRMDEGPVTFYVGCDPTGPS
                     LHIGHLVPEFALMHLRNYGHRGIALIGGGTARIGDPSGKIEMRKMLSYEQIDENVEKI
                     RAQLDRIIGFDDKTALSDNNKFWLADLNYIDFLRDIGAHFSVNRMLTFEAYKKRMETG
                     LTFIEFNYQLLQSFDFLKLLERHDCILQIGGDDQWGNIVAGVDLIRRKTGKESFGLTF
                     PLVTRSDGKKMGKTEKGALFIDKELTPVFDFFQYWRNIDDADIAKFFKLFTFLPLEEI
                     DTIIAGDVNKAKERLALEITTIIHGREEAETALAGAKAAFGGGGDKTALPTTELPKNL
                     IEEGLGIIDLFFEAKLGGSKSDIRRLIDQGGAYYTDKDGKEQQCRDNKMLVNVSFLDD
                     DGELIIRAGKKKYMRLTFR"
     gene            2615..4636
                     /locus_tag="PKW26_06025"
     CDS             2615..4636
                     /locus_tag="PKW26_06025"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOQ93159.1"
                     /translation="MIENNFGFVRLAVASPSLILGQTKKNAQTILSLVREAESKKIAT
                     LVFPHLSLTGLSLGDLLFQDSLLDASREMLLWIAKETLGCPVLFSLGLPFFFENRFYS
                     VQAWLHSGTILALIPSSADSHFFRSSNRLSPAKRIFLSEDHPAIPFSPAIIIQDSALP
                     FFTLTSITEDAIYAPFSPALLASKNGARIIAHHASDKSLVGKGSEKELFLKVLSKQGN
                     FACLSAAAPPSESTSEFVYSAHNLILERGRLLRESFLQENKIISCEIDLDLLAREEAR
                     KKRLENSAFHFAEEGRDSYEIIRHPILKDVRIGILERSIKKDVFIPELVDKKARYDAI
                     FQTQVLALVKRLKHISCEKMILGLSGGLDSCLAFIVAVKTCRYLGLDAKNILAISMPG
                     FGTTSKTKTNAEKLASAYGASFLEISIEKAVRQHFLDIGHDSTVENIVFENAQARERT
                     QILMDLANKESALLLGTGNMSELALGWTTYNGDHMSMYALNSGIPKTLVKDLVQFEAD
                     EILKIAKTAEKEDDAEAKKYMERAQVLAAVLDTPISPELLSPVSGAIAQKTESLIGPY
                     ILHDFFMYYLLRFGFSPKKIFFLAEQAFIFQEKEPLFNKKEILSWLRVFYSRFFSQQF
                     KRSCAPDYVSIGSLAFSSSSWSMPSDAVADIWLAELDDLEKNVDKALQK"
     gene            complement(4680..6497)
                     /gene="argS"
                     /locus_tag="PKW26_06030"
     CDS             complement(4680..6497)
                     /gene="argS"
                     /locus_tag="PKW26_06030"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOQ93160.1"
                     /translation="MTDKTENTNMKQIAKNIVLQALEAIAAEKNVAIQINETNLIMET
                     PPNPDMGDIGFPLFPFAKALRMGPPQIAEALLEKVKAIADTKKIGIFRAVGPYLNLYL
                     NKASSISSLLQSIEKDGSEYGSKNTAGEKPLANRNIMLEFSSPNTNKPLHLGHLRNDA
                     LGESVSRILKKLGANVFKVNIINDRGVHICKSMLAYEKFHRGKTPESLNVKSDRFVGD
                     CYVDFNNYAKTNPQAEEEAQKLLRKWEAGDKEVTDLWKTMNTWAFEGIKETYERTNVS
                     FDKFYFESQTYLKGKEEILKGLEEGLFYKDDDGAIWIDLEEIKLDKKVLLRKDGTALY
                     ITQDIGTAIERHQDWPFDQLVYVVGNEQIYHFKVLFYVLEKLGFNWAKQLYHLSYGMV
                     NLPEGKMKSREGTVVDADDLIDSLRDDALAEIREKGRETEVGDAKAVAEKIALGALHY
                     FLLQVSASKDMIFDPKESLSFNGNTGPYLQYMGARISSILKKAEKERGNTKPQTDDAV
                     TTIDTSLLSHAAEWELAKHLELFPESVEKAGRDFDPSIIAIYAYDLAKLFSRFYHDCP
                     ILQAENKALSEARLALVQAVKEVLMSAMDLILVPFLETM"
     gene            complement(6514..8268)
                     /locus_tag="PKW26_06035"
     CDS             complement(6514..8268)
                     /locus_tag="PKW26_06035"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HOQ93161.1"
                     /translation="MKESNSFERLASTISQEERASLLKKLKTSEKEEELGQLKPITDE
                     KEYTENSIQELLKNENLFTRIYLFIKSLFTSENVEYVYNDMLLKGRAKSIEKNHPALL
                     NYKEKIFTTGLYNQIYELSLVAHFFKPFINSYEDNRSHFYITLSSIVVPELTKRMDEE
                     ANPHNSKMPPEDIISRRSSFSKKIDEIINDISVSEKNTLLITIRAVEWLSAFCDLPFN
                     KILAKFTALPGSDFSLPFENGTKEFALFAKVLSTGTRFPLEMFETFYILQNTKKKIQS
                     QNTTNPESETTAFADTADEEILKDFIEKSSEQIQLIKMYIQAVPVIDIARIAHNNILW
                     QPETLEKGEGWLIQLKSYWKKFFDDTWEQWLISVKKNMLLEQIKNLIGEENYPKLPYK
                     PWAKIESIFSFQFEHSLEFIFAFFKTSYPKHAAVLKEVLLNGDFIQRDNKTEFTDTYN
                     ALGQIEKSLHLLNEKLSFKGTYGQAFTKLHHEALITVQGQAKLKALLHSVEGEIKTLV
                     LHFADSCRSMTSLIGGILHEEKNLRYASLKNIAEIWGNKNIEFRNQLHETRVDFLDSL
                     EIIKELDSLSLLETSAKN"
     gene            8407..>10528
                     /locus_tag="PKW26_06040"
     CDS             8407..>10528
                     /locus_tag="PKW26_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ93162.1"
                     /translation="MNKMHSILSSLHKTDALLAFVLKKARFAFFVFDVTEEILEIHKK
                     SAKKQDTFLQFFNFYNTELKNSGFDENYILEFKQYIKKFANDKKGDSFEYLGDHIDDV
                     FQWHSLSCFSYIDPLSTKQYIIGLSQALATDEPLNRFEEDLLSQKDDSKTVISSFKIN
                     ISRQMIEEYNRFNGKEIKMKAISFDEANKLDFFKVFRNKDTFPFDFKNLDKFTELVKN
                     GSKQFVFERRVLIDNVFVWVEIHILLTKQKTTNEIFAHCYILDINRLKILSILTEQHV
                     FFEYDHLAYIEVDTGTFYYVQYPLESSLLKKSEFDKYISLFLKAIVVEEDRTYAKEQL
                     VLKKIVKALEERRFYTIYLGVYGKKNEYQRKKFTFTFVNNEKNILALSVSDVTDFYKA
                     EEEKRESLHNALEMAQNANFAKTEFLSRMSHELRTPLNAIMGLVILAQQKAIENKELL
                     ENLTKINSSAHYLLSIINDVLDMSRIESGKLHMSKGLIVIKELVAKVNAIIESQAKQK
                     EIQYTWEIAESVYHSFVGDELKLQQVLINILGNAVKFTPDGGKVHLQIEQVFFFNTEQ
                     KRKLRITISDTGIGIEKKFLPHIFDAFSQDLLKTRSELAGTGLGLAISKNLITIMGGT
                     ISVESVEGEGTSFVIELVLPIYDEEMENKKNNLARFENQGYMLPNAVDTTAIPAKEDN
                     GLVQMNTSFYRGNRILLVDDNRMNL"
BASE COUNT         3046 a         1899 c         2114 g         3469 t
ORIGIN      
        1 agtcatttgg tttggttttt cacaaaaagg aaaaaccctc ggcctaagtc tcactggttc
       61 aacgctctct ggtgttgcct ggggtgattt tatgcgcgaa gcacatgagg gctaccctta
      121 caaggccttt agcgaaccgc aaacaggtct cgtaaaggca actgtatgct cggtttcagg
      181 actcttactt acagaagctt gtggaaaatc cgcgacaaca caatactttc tcgagggaac
      241 gcaacccctt acgatgtgcg attatcatat aaatcgagaa acagctcgcg aaatcggtgc
      301 tttgcgtttg cttgacgcaa gatatcagtc tggagcacaa gaagcgcaga tacaagatac
      361 tggcggcttg gtcctagacc tttcgttttt gaatattaaa acgagtgaag attttgatac
      421 ctacgaaaaa gaaaaagatg cgaggtctga atcggatgca atatacaatg attttttctt
      481 tacgatagat gatcaagatg aagatagcga tgaagatgat gaaatgagta attttttgct
      541 tgactaaaaa gcaattcaac tttaacctag attagggtga atgatgatag tagcaatttc
      601 agatataaag ataaaaaaaa ggatacgaaa agacctcggt gatatcgacg ctttgatgga
      661 aagtttagag caatacggtc ttttgaatcc tattactgta aacacaaaaa atgaactcat
      721 tgcgggacat cgtcgccttg aagcagcaaa gcgactcggt tggcaaaata tacatgcggt
      781 gcaaatagat atacgagacc cactcttgtt attagaattg gagatggaag aaaatatgca
      841 aaggcttgct tttagcgatg cggaagctca agacggctat gcaaaactag aaaagttgag
      901 aaatccacat tttataaaaa aatggtggcg caatatcgct ggatttttct cagatttatt
      961 ttcatcaaat gcaaaagagc gtcttggcaa aaaggtcttt caagcaagaa aatacctgtg
     1021 gctatttccc attggtatct ttatgctcat cgtttcaggg ttttggaata aaacaaggct
     1081 gagtggcagt atttttaaaa cgctctttga cctaagctcg ctcattatac tgctttttgc
     1141 ctgtttttca cttgtacgcc ttatctactt aaaacgcatt aacgaaaagt aagccgcata
     1201 tatttctttt taccagcacg tataatgagc tctccatcat cgtcgagaaa ggagacattt
     1261 acaagcattt tattgtcgcg gcattgttgt tctttgccat ctttgtcggt gtagtatgcc
     1321 ccgccttggt cgatgaggcg gcggatgtcg cttttacttc cgccgagttt tgcttcaaaa
     1381 aagaggtcta taataccaag tccctcttct atgaggtttt tgggaagttc tgttgtggga
     1441 agtgcagttt tatcgccgcc gccgccgaat gcagcctttg ctcctgcaag ggctgtctca
     1501 gcttcttctc tgccgtggat tattgtggtt atttcgaggg cgagcctttc cttcgccttg
     1561 tttacatctc cagcgattat tgtatcgatt tcttcgagcg gtaaaaaggt aaataattta
     1621 aagaattttg caatgtcagc gtcatcgata tttcgccagt actggaagaa atcgaaaacg
     1681 ggtgttagtt ctttgtctat aaacaaggcg cctttttcag ttttgcccat ttttttacca
     1741 tcggaacggg ttaccaacgg aaaggtgagc ccgaagcttt ctttccctgt ctttcgtcga
     1801 atgagatcga cccctgcgac gatattgccc cattggtcgt ccccaccgat ttgcaatata
     1861 caatcgtggc gttcgaggag tttgagaaaa tcgaagcttt gtaacaattg atagttaaat
     1921 tctataaagg ttaagcccgt ttccatgcgt tttttatagg cttcaaaggt aagcatacgg
     1981 ttaaccgaaa aatgtgcccc aatgtcgcgt agaaaatcta tataattaag atcggcgagc
     2041 cagaatttat tattgtcact gagggcggtt ttatcgtcaa agcctatgat gcgatcgagt
     2101 tgtgctcgta ttttttcgac attttcgtcg atttgctcat agctaagcat ttttcgcatt
     2161 tcaatttttc ccgatgggtc gcctattcgt gcggttcccc caccaatgag ggcgattccg
     2221 cggtgtccat agtttcgtaa gtgcatgagg gcaaattctg gcacaaggtg cccgatgtga
     2281 aggctcggtc cagtagggtc gcagcctaca taaaaagtta cgggaccctc gtccatgcgt
     2341 tttgaaagtc cttccaaatc ggtacattgt tgaaaaaatc ctctgtccat gagtgtttgt
     2401 agtgcttcgt tcataatttg ccttccgtag tgaaaaatct attttatata cgcgcaaaga
     2461 gctcgttgtt ttttgcgctt atatttgcca ttttggctct attctcctag tctatcaatt
     2521 tttcgccctc tcttcaagca gaaaaacatg ctcttttttt tgctttttta taatatgctt
     2581 gctcgtttct ctattttcct tgtatactat agatatgata gaaaataatt ttggttttgt
     2641 tcgccttgcg gtagcaagtc cctctcttat tttggggcaa acgaaaaaaa atgcccaaac
     2701 tatattgagt ctcgtccgag aagctgaaag taaaaagatt gctaccctcg tttttccgca
     2761 tctttctttg actggtctaa gccttggcga tttgcttttt caagacagcc tcttagatgc
     2821 ttcaagggaa atgcttctat ggatagcaaa ggagactctg ggctgtcctg ttttattttc
     2881 cctcggtctt cccttttttt ttgaaaatag attttactct gtccaggcat ggcttcatag
     2941 cggaactatt ctcgccctta ttccgagctc tgctgacagt cattttttta gaagttctaa
     3001 cagactgtct cctgcaaaaa gaattttttt aagcgaagat catcccgcta ttcccttttc
     3061 tccagctatt attatccaag atagtgctct gccttttttt actcttacat cgattacaga
     3121 agatgcaatc tatgctcctt tttctccagc ccttttggca agcaaaaatg gggcgcgcat
     3181 tatagcacat catgcgtcgg ataaatcctt ggtgggaaaa ggctctgaaa aagagctttt
     3241 cttaaaagtt ttgagcaagc agggaaattt tgcctgcctt tctgctgctg cccctccctc
     3301 tgaatctaca agtgaatttg tctacagcgc tcataatctc atcctagagc ggggaaggct
     3361 ccttagagaa tcttttttgc aagaaaacaa gattatatcc tgtgaaatag atttggactt
     3421 gcttgcaagg gaagaagcgc gtaaaaaaag acttgagaat agcgcttttc attttgctga
     3481 agaagggcgg gatagctatg agattattcg ccatcctatt ttgaaagatg tgcgcatagg
     3541 aattttggag aggtccataa aaaaagatgt ttttataccc gagcttgtag ataaaaaagc
     3601 gcgctatgat gctatttttc aaacacaggt tttggccctt gtaaaacgtc taaagcatat
     3661 ttcttgcgaa aaaatgatcc tcggtctttc tggtgggctt gactcatgcc ttgcctttat
     3721 tgttgcagta aaaacctgtc gctatcttgg actcgatgca aaaaatattc ttgcgattag
     3781 tatgcctggt tttggtacca cttctaagac aaagacaaat gcggaaaaac ttgcttctgc
     3841 ttatggggcg agttttcttg aaatctccat agaaaaggca gtgaggcaac attttttaga
     3901 tatcggtcat gatagtacgg tagaaaatat cgtttttgaa aacgcacaag cgcgagagcg
     3961 cacgcagata ttgatggact tggcaaataa agaatcggca ctgcttcttg gcacgggaaa
     4021 tatgtcggag cttgcccttg ggtggacaac ctataatggc gaccatatgt ctatgtatgc
     4081 gctgaatagc ggtatcccga aaacgcttgt aaaagacttg gtgcagttcg aagcagatga
     4141 gatattaaaa atcgctaaaa cagcagaaaa ggaagatgat gcagaagcaa agaagtatat
     4201 ggaaagggcc caggttttag ctgctgtttt ggatacgcct ataagccctg aattattgtc
     4261 tcctgtttct ggtgctatcg ctcaaaaaac tgaaagcctt ataggtccct atattttgca
     4321 tgattttttt atgtactatc ttttgcgttt tggtttttca ccaaaaaaaa tatttttttt
     4381 agcagagcaa gcttttattt tccaagaaaa agagcctctg tttaataaaa aagaaatact
     4441 atcttggctt agagtttttt atagtcgctt tttctcccag caatttaaac gctcctgtgc
     4501 tcccgactat gtgagcatag ggagccttgc cttttcatcg agttcttggt ctatgccaag
     4561 cgatgcggtt gcggatattt ggcttgcaga gctagatgac ttggaaaaaa atgtcgacaa
     4621 ggctctgcag aaatagagtc tgagaaaaag tagattcaaa aagcccgaaa ggttatgctt
     4681 tacatcgttt ccaagaaggg cacgagaatg aggtccatcg cagacattaa aacttctttt
     4741 accgcctgaa ccaaggcaag gcgcgcctct gaaagggctt tgttttccgc ttgcaaaata
     4801 gggcagtcgt gataaaagcg actgaaaagt tttgccaagt cataggcata gattgctatg
     4861 atcgacgggt caaagtccct gcctgctttt tcgacgcttt ctggaaagag ctcgaggtgt
     4921 tttgcaagtt cccactctgc ggcatggctt agcaagcttg tatcgattgt agtgacggca
     4981 tcgtctgttt gaggctttgt attgcctcgt tctttttcag ctttttttag tatagaagaa
     5041 atacgtgcac ccatgtactg gagatagggc cctgtgtttc cgttaaaaga aagcgattct
     5101 tttgggtcga agatcatatc cttcgatgca cttacttgca aaagaaaata atggagggca
     5161 ccgagcgcaa ttttttcagc aacagccttt gcgtctccta cttcagtttc cctacctttt
     5221 tctcgaattt cagcaagggc atcatctcgt aggctatcta tgagatcgtc ggcatcaaca
     5281 acggttccct cgcggctttt cattttcccc tcagggagat taaccatgcc gtaggaaagg
     5341 tgatagagtt gttttgccca gttaaagccg agcttttcca aaacatagaa taaaacctta
     5401 aagtgatata tttgctcatt tccaacaaca tagacaagtt gatcgaaggg ccaatcttga
     5461 tgccgctcaa ttgcggtacc gatatcttgg gttatgtaga gggcggttcc gtcttttcgc
     5521 aataagactt ttttgtcgag ttttatctcc tcaaggtcta tccaaattgc accgtcgtcg
     5581 tctttgtaaa aaagcccttc ttcaagacct ttgagaattt cttcttttcc ctttaggtag
     5641 gtttgacttt caaaataaaa tttgtcaaaa gacacattgg ttctttcata ggtttctttt
     5701 atgccttcaa aggcccaggt attcattgtc ttccacaagt cggttacttc tttgtctcct
     5761 gcttcccatt tgcgaagtag cttttgtgcc tcttcttcag cttgcggatt ggtttttgcg
     5821 taattattaa aatctacata acaatcgcct acaaagcgat cactttttac gttaagactt
     5881 tcgggtgttt tacctctatg aaatttttca taggcaagca tactcttaca aatatgcaca
     5941 cctcgatcat tgataatatt taccttgaaa acatttgcac cgagtttttt taatattctt
     6001 gaaacagatt caccgagtgc atcatttcgc aaatgcccaa ggtgcaatgg tttgtttgta
     6061 tttggacttg aaaattcaag cataatattt cgatttgcaa gaggtttttc gcctgcggta
     6121 tttttacttc cgtattcaga gccatccttt tcgatgcttt gaagtaagct cgaaattgaa
     6181 ctagccttat ttagatagag attgagatag ggtccgacgg ctcgaaaaat gccgattttt
     6241 tttgtatcag cgatggcttt tactttttca agaagcgctt ctgctatctg cggtggtccc
     6301 atacgaaggg ccttggcaaa ggggaagagc ggaaagccta tatctcccat gtctgggttt
     6361 ggcggagttt ccatgataag atttgtttcg tttatttgaa tagcgacatt tttttctgca
     6421 gcgatggctt caagtgcctg tagaacaatg ttttttgcga tttgtttcat gtttgtattt
     6481 tctgttttat cggtcataat aaaaagaatg gtatcaattt tttgcgcttg tttcaagaag
     6541 agagagcgaa tccaattctt ttatgatttc taaagaatcc aaaaaatcaa ctcgtgtttc
     6601 atgcaattga tttcgaaatt caatgttttt gttgccccag atttctgcaa tgtttttgag
     6661 gcttgcgtat cgaagattct tttcctcatg taaaatgccc ccgataagag aagtcatcga
     6721 acggcaggaa tcagcaaagt gcaaaacaag tgtttttatt tcgccttcta ctgaatgcaa
     6781 tagtgctttt agctttgcct gtccttgcac tgtgataagg gcttcgtgat gcagttttgt
     6841 gaacgcttgc ccatatgtcc ctttgaatga taatttttcg tttaataagt gtaggctttt
     6901 ttcaatttgc cctagagcgt tgtaggtgtc ggtaaactct gttttattat ctctttgaat
     6961 aaaatctccg ttaagcagta cttctttgag aaccgcagca tgttttggat agcttgtttt
     7021 aaaaaaagcg aatataaatt caagagaatg ttcgaactga aaggagaaaa tagattctat
     7081 tttcgcccag ggtttatagg gtagtttggg gtagttttct tctccgataa ggttttttat
     7141 ctgttctagg agcatgtttt ttttcactga tataagccat tgctcccagg tatcatcaaa
     7201 aaactttttc caataggact tcagttgtat aagccatcct tcgccttttt caagagtttc
     7261 aggctgccat aaaatgttat tgtgtgcaat tcgtgcaata tcgattactg gtacagcttg
     7321 aatgtacatt tttataagct gtatttgttc agagcttttt tcaataaaat cctttaagat
     7381 ttcctcatcc gctgtgtcgg caaatgctgt cgtttcactt tctggatttg ttgtattttg
     7441 gctctgtatc ttcttctttg tattttgtaa tatataaaag gtctcaaaca tttcgagggg
     7501 gaaacgagtg cccgttgata gtacttttgc aaaaagagcg aactcttttg tgccgttttc
     7561 aaaggggagg gaaaaatcag agccaggtag tgctgtaaat tttgcaagta ttttgttgaa
     7621 gggtaaatca caaaatgcgc tcagccattc aacagctcgg atcgtaatga ggagagtgtt
     7681 tttttctgat acagaaatat cattgataat ttcatctatt ttttttgaaa aactacttcg
     7741 tcgtgagata atatcttctg ggggcatctt tgagttgtga gggtttgctt cctcgtccat
     7801 gcgttttgta agttcaggaa cgacaatgct actcaatgtt atataaaaat gggagcgatt
     7861 atcttcatat gaatttataa agggtttaaa aaaatgtgca actaatgaaa gttcgtagat
     7921 ttgattatat aaaccagtcg taaaaatttt ttctttatag tttagcaaag caggatgatt
     7981 tttttcgatg cttttcgctc taccttttag tagcatatca ttgtaaacat attcaacatt
     8041 ttctgaggta aatagggatt ttataaaaag atagattctt gtaaaaagat tttcattttt
     8101 taaaagctct tgtatactgt tttcagtata ttctttttca tctgtgatgg gctttaattg
     8161 accgagctct tcttcttttt ctgaagtttt tagttttttt aaaagagagg ctctctcttc
     8221 ttgagatatt gttgatgcaa ggcgttcaaa tgaatttgat tctttcatat gctactattg
     8281 taagcgagca agtgattaag gtcaagaaaa actctgcaag tatttgccga aaaacaaagc
     8341 ttgctattct taaaaataca aactatacta tatttgtggt tggctcttat ttagtatggt
     8401 gaaaatatga ataaaatgca ttctatatta tcttctttgc ataaaacaga tgcattactt
     8461 gcttttgtct taaaaaaagc aaggtttgca ttttttgttt ttgatgttac agaagaaata
     8521 cttgaaattc ataaaaaatc tgctaaaaaa caagatactt ttttacaatt tttcaatttt
     8581 tataacacag agctaaaaaa ctctggtttt gatgaaaatt atatcctcga atttaagcaa
     8641 tatattaaaa aatttgccaa cgataaaaaa ggggattctt ttgaatattt aggcgatcac
     8701 atagatgatg tatttcaatg gcattcgctt tcatgcttta gctatataga tccactttct
     8761 acaaagcaat acattatagg tttatcacaa gccttagcga cagatgaacc tttaaatcgt
     8821 tttgaagaag acttattatc gcaaaaagat gattcaaaga ctgtcataag tagctttaaa
     8881 ataaatatat caagacaaat gatcgaggaa tataatcggt ttaatggaaa agaaataaaa
     8941 atgaaggcta tttcctttga tgaagccaat aagctcgatt tttttaaggt tttccgaaat
     9001 aaagatactt ttccatttga ctttaaaaat ctggataaat ttacagagct tgttaaaaat
     9061 ggatctaaac aatttgtatt tgagcgaaga gttttaatag acaatgtctt tgtttgggtt
     9121 gaaatacata ttcttttgac aaaacaaaaa acaacaaatg aaatcttcgc acattgttat
     9181 atacttgata ttaacagatt aaaaatactt tctattttaa ctgaacagca tgtgtttttt
     9241 gaatacgacc atcttgctta tatagaagtt gatacgggaa ctttttatta tgtacagtac
     9301 cctttggaat catctctttt aaaaaaatca gaatttgaca aatacatatc tttatttttg
     9361 aaggctattg ttgtggagga agataggact tatgcaaaag agcaattagt tttgaaaaaa
     9421 attgtaaagg ctcttgagga gcggcgtttt tatactatct atcttggagt ttacggcaaa
     9481 aaaaatgagt atcaacgtaa aaaatttaca tttacctttg taaataacga aaaaaacatt
     9541 ctcgctcttt cggttagcga tgttactgat ttttataaag ctgaagagga aaaaagagaa
     9601 tccttgcaca atgcacttga aatggcccaa aatgcaaact ttgcaaaaac agaatttttg
     9661 tcaagaatgt ctcatgagct gcgaacacct ttaaatgcta tcatgggctt ggttatttta
     9721 gcacaacaaa aggctatcga aaacaaggag cttcttgaaa atctaacaaa aataaatagt
     9781 tcagcacatt atttactttc tattatcaat gatgtattgg atatgtctcg tattgaaagt
     9841 ggaaaattac atatgtcaaa gggacttatt gttataaaag aacttgtggc aaaggtaaat
     9901 gccattattg agtctcaggc aaaacaaaaa gaaattcagt acacatggga aatagcagaa
     9961 tctgtctatc actcttttgt tggagatgag ctcaaattac agcaagtact tattaatatt
    10021 cttggaaatg cggtaaaatt tacgccagat ggcggaaaag tacatttaca gattgagcaa
    10081 gtttttttct tcaatacaga acaaaaacga aaattacgca ttactatttc tgatacaggc
    10141 ataggcattg aaaaaaagtt tttgcctcat atttttgatg cattctcaca agatttactt
    10201 aaaacaagaa gtgaacttgc cggaacgggt cttggtcttg caattagtaa aaatcttata
    10261 acgataatgg gtggtactat ttctgtagaa agcgtagaag gcgagggcac aagctttgtt
    10321 atagagttgg tgcttcctat ttatgatgaa gagatggaaa acaaaaagaa caatttggca
    10381 aggtttgaaa atcaaggata tatgcttcca aatgctgtag atacaaccgc tattccagct
    10441 aaggaagata acggtttagt tcaaatgaat acttcttttt acagagggaa tcgcatactg
    10501 cttgttgacg ataacaggat gaacttag
//
LOCUS       DAOSSW010000061        10526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_6161_length_10526_cov_5.857034, whole genome shotgun sequence.
ACCESSION   DAOSSW010000061 DAOSSW010000000
VERSION     DAOSSW010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 10526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10526
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6161_length_10526_cov_5.857034"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2185)
                     /locus_tag="PKW26_06045"
     CDS             complement(<1..2185)
                     /locus_tag="PKW26_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOQ93163.1"
                     /translation="MLKKYCAFISFLLLCALCLISAENNPKTTIKIGFLPNYGISFHG
                     ETNKLEGHTVEYFAELSKFTGWSYEFVPLEWEEGLKRLKDGSIDFFGPMQKTPSREKD
                     YLFSNRELGFEHSLLYAKKDSPLLFQDFESFKGIRIASFPTNAFNEGLERFAQKNNFS
                     YDFVLTESDTGIKELQEGKFDALLYSNLVEIPNTTIIAKVETLPYYFATAKKNSDLIE
                     QLNEAMADLDFNKPYFTAELTKKYFNDPSINNIAFSKNELGFIKDHPVLRVVHNPNLK
                     PVEFFNSKTKKHEGISISILKELSSILGITFESIESKSYAESIQMIQDGKVDLITGYA
                     ENAKNYSDIQLSDTYYEIPVVLLKKQKETSKTLSAAIPAYYTYPVNKIKELYGHYNFA
                     LYEDFNACLEAFLKQEVDATFINSASFDSVIKDIPLDSYFVVYTGISYPLQLGLSPYF
                     DTVALSTFNKAITKISEETKQTIVFSHTLKKNYNVDFLTLLRQYVLHIIIGLVILFLL
                     FGITILYMTHKSKKRLQSIAFHDEQTGLLSLSKFKRDAHKLLYYANENDYMMMSLDID
                     NFKYINQSYGYEIGTVIISNLGLYLKDLESPQTLVCRSHNDVFLIFMKRKPWTEILSF
                     FDNIVKIKNSFNELLSENHIVTFSAGVYTVSNLSLSINDIIDKATIARKSIKGLHHTS
                     QIVEFTEKMEKEIQWHKEITLTMNQAYTNNNFVVNYQPKFLIADNS"
     gene            2313..3110
                     /gene="flgF"
                     /locus_tag="PKW26_06050"
     CDS             2313..3110
                     /gene="flgF"
                     /locus_tag="PKW26_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HOQ93164.1"
                     /translation="MIRGWYIGASGMNAQQARLDAISNNLANVDTTAYKKDVAVNKSF
                     PELLLRRLNDDGVYKTYFGSADAAPIIGKIGLGVETNELYTILEQGSFKQTGSFTDFA
                     LQEEGFFVVETPQGERYTRNGNFMVGKEGYLQTKEGYPVLGDKGRIYVEDIKFVAKED
                     GVLYSEEDMEEIDRLKLVRFDNERYLKKIGSNLYQDTNISGEAYSAEGFERPIVLQSY
                     TETSNVNVVNEMVQMIDVNRAYEANQKTIQAGDSMMSTLWTKVASIR"
     gene            3165..3959
                     /gene="flgG"
                     /locus_tag="PKW26_06055"
     CDS             3165..3959
                     /gene="flgG"
                     /locus_tag="PKW26_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOQ93165.1"
                     /translation="MVRSLWTAATGMNGQQFQIDTISNNLSNVNTTGFKKQRAEFEDL
                     VYQTVKMAGTPATEDTVTPVGIQMGNGVKLASTQRMFNQGSMQNTENATDLALMGEGF
                     FRVQKYDGSFAYTRDGSFKIDSNGQLVTSNGFRVMPEVIMPEGFNINSLQISDDGRVT
                     VKVFGNDEPLDIAQIEIYRFPNEVGLEADGDNLFLATNASGTAIASRPGFEGAAITKH
                     KFVEMSNVSVVNEMVQMIVAQRAYEFNSKAIQTSDTMLSTAVNLKR"
     gene            3995..4564
                     /locus_tag="PKW26_06060"
     CDS             3995..4564
                     /locus_tag="PKW26_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HOQ93166.1"
                     /translation="MAAIDLVYNTTNLSHMGNFTALSGGLSSVTKGDAIAEAAQLSKE
                     VSDFQNLLDSITREAETKESAVPSSRLNGDYLSSFSSATNTADTRAKPQGEAANSANP
                     HIPSHSTRTIDKSSALYEKSLELESYFVKIMLSSMKNTINLSEINGKESYASSMYTDM
                     LYDEYNVILTKNAGFGLADQIYLQLSDDN"
     gene            4629..5756
                     /gene="trpS"
                     /locus_tag="PKW26_06065"
     CDS             4629..5756
                     /gene="trpS"
                     /locus_tag="PKW26_06065"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOQ93167.1"
                     /translation="MKEDNKTETETETKLETSISNATYEKAKEKSAKLEADIIANPQK
                     YRVLTGDRPTGRLHVGHYFGSLQNRVRLSKLGVPTCILIADYQVLTDHDAFDEIAQNT
                     KQLVIDYIASGIEPSENIIIYPHSYVPEANQLMLPFLTLVSNSELNRNPTVKEEIQAG
                     GLKTVNAGMYTYPVHQACDILFCKGTVVPVGKDQLPHLELTRLIARRFNEKFSPKKPI
                     FPLPEPLLSKAPTILGLDGSQKMSKSRGNAIMLSATEDETAALIKKAKTDADRNISYD
                     PENRPEVANLLMMIHLCTGEAPEAIAARIGDGGGGALKKMLTEALNETLRPIRQKRSE
                     LEKDDAYIKEVLQNGIASARKMAIETLDEVRRAMNMEINSF"
     gene            complement(5753..>6328)
                     /locus_tag="PKW26_06070"
     CDS             complement(5753..>6328)
                     /locus_tag="PKW26_06070"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HOQ93168.1"
                     /translation="DIGKPDVRKESLKSLDPKRPAKLSTIVSFHNHEKASVEKTRTLL
                     QRLRFPNKLIQEVCHLIQHHMISYESAWSDAAVRRFVVRVGVEPLEKLFELHLCDSWA
                     TKGEKPGELYFSVIREFRERIEKVLDENTALSLQDLAINGRDLLQEGYKGKIIGEILN
                     ELFSSVTENPSVNKKALLLQRAKELYKKEPF"
     assembly_gap    6329..6428
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6429..6985)
                     /locus_tag="PKW26_06075"
     CDS             complement(<6429..6985)
                     /locus_tag="PKW26_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HOQ93169.1"
                     /translation="MASIAIPKELQKMNRFFEEAGFEAYLVGGAVRDSLRGDFASDWD
                     LATNARPEDVMKIFKKVIPTGIDHGTVTVHFMGRHIEVTTFRTETDYRDSRHPSSVQF
                     NASLGEDLSRRDFTINAIAANLKTGRIIDPFNGQADIKNRCIQTVGNPIERFTEDALR
                     PIRAIRFSSQLSYEISPETYDAIKKV"
     gene            complement(7053..7877)
                     /locus_tag="PKW26_06080"
     CDS             complement(7053..7877)
                     /locus_tag="PKW26_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93170.1"
                     /translation="MKKFIMSSIILLIFAAVIFFIGWVQLAIPAGSSAVLISKTGGIH
                     PQTLEAGSFIWRWERLLPTNTEMRSFSIYPLEVKSEIQGSLPSSDIYREFLEGRPDFS
                     YAFTIRSQIKLRDSALPQFVRRTNALGQDELNQFLEKEAKKINQRAVSLLLQKTEASA
                     DFLAFIDTETLIKKLSEENSEIEILSLEIESQKTPDTELYLLAKEAYFHYQEAVRKSL
                     IDIAETEASKSAEDFLQIERFAKWGKVLQDYPILIDFIAVSRDDAASALEALKKMR"
     gene            8434..8748
                     /locus_tag="PKW26_06085"
     CDS             8434..8748
                     /locus_tag="PKW26_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93171.1"
                     /translation="MKKIQYDRILKDCFWDLNLSENDIKEIIQGDDERKKMMLFEKIL
                     LSSTAFFKDLEIFSKNELKKLLENYTLPSFNKDFAFRRKNMAEVYFFDKALLISELQW
                     IA"
     gene            8736..9389
                     /locus_tag="PKW26_06090"
     CDS             8736..9389
                     /locus_tag="PKW26_06090"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOQ93172.1"
                     /translation="MDSLNYKKLYALQDEVLDLVFATEKEFYLTGGTCLSRFYSEKRY
                     SDDLDFFTNQSPRYSFALRNIKKVLMEHFSLSVEVEAKDFSRYKIDSLLQIDFVNDIS
                     FRYKEPIINEQNYLIDNLENILSNKISAIIGRDNPKDIFDLYLIWKISQVHWAHILEA
                     AHNKTGFTNEELIIRLKTFPSQMLSSIKLTEPHFLDNFEKEFPLLISEIIEIDSRGY"
     gene            9923..>10526
                     /locus_tag="PKW26_06095"
     CDS             9923..>10526
                     /locus_tag="PKW26_06095"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOQ93173.1"
                     /translation="MNYTVTVPNSVTSLTVTGTKSHANATMDLSDGKLEFTNLSVGTS
                     SEQTIKVTSEDGSATNEYKVKVLRQYIEMLPVPAGSFQRDETTTNISKITNSYYLGKY
                     PITRQQFFDVMGKDPSDTAKSSGMNDPVQMVNWYHAIAFCNKLSLLEGLSPAYTVGGV
                     SDWAALAFSSIPTSENKNWDKAECDWEANGYRLPTKKEWMW"
BASE COUNT         3424 a         1945 c         1903 g         3154 t
ORIGIN      
        1 aggagttatc tgcaattaaa aatttaggtt ggtaatttac tacaaagtta ttgtttgtgt
       61 aggcttgatt cattgtaagg gtaatttctt tatgccattg tatttctttt tccatttttt
      121 ctgtaaattc aacaatctga cttgtatgat gtaagccttt tatcgatttt cgagcaattg
      181 ttgccttgtc aataatgtcg tttatagaaa gactcaagtt tgaaacggtg tatactccgg
      241 cgctaaaagt aacaatatga ttttcagaaa gtagttcatt aaacgagttt tttattttta
      301 ctatattgtc gaaaaaagaa agaatttcag tccagggttt tcttttcata aagatgagaa
      361 aaacgtcgtt atggctgcgg caaactaagg tttgaggtga ttctaagtct tttaggtaga
      421 gcccaagatt tgaaataata acagttccaa tttcgtagcc ataggattga tttatatatt
      481 taaaattatc tatatctaag ctcatcatca tataatcatt ttcattcgca taatagagca
      541 atttatgcgc atctcttttg aacttagata aagaaagtaa accagtttgt tcatcgtgaa
      601 aggcaatgct ttgtaatctt ttttttgatt tgtgagtcat gtacagaata gtaatgccaa
      661 aaagcaaaaa gagaatgaca agtccaatga taatatgtaa aacgtattgg cgtagaagtg
      721 tgagaaaatc tacattataa ttttttttca aggtatgtga aaacacaatt gtctgttttg
      781 tttcttcgct tatttttgtg attgccttgt taaaagtaga tagggcgaca gtatcaaaat
      841 agggcgaaag accgagttgc aaaggatatg aaatgccagt atagactaca aaatagctgt
      901 ctaaggggat atcttttata actgaatcaa atgaagcaga atttataaag gttgcgtcaa
      961 cttcttgttt taaaaaggct tctaagcaag cattaaaatc ttcatacaag gcaaaattat
     1021 aatgtccgta caattctttt atcttgttta caggataggt ataataggca ggaattgctg
     1081 ctgaaagagt tttgcttgtt tctttttgtt tttttaataa aacaacaggt atttcataat
     1141 aggtatcact caattgaata tcgctataat tttttgcatt ttctgcatag ccagtaatga
     1201 ggtctacctt gccatcctgt atcatttgaa tactttctgc gtatgatttt gattctatgc
     1261 tttcaaatgt aattcccaag atcgaactta gctcttttaa tattgaaata cttatgcctt
     1321 cgtgtttttt tgtttttgaa ttaaaaaact caacgggttt taggttggga ttatgcacaa
     1381 cacgcaagac agggtggtct ttaatgaagc ctaattcatt ttttgaaaag gcaatattat
     1441 ttattgaagg gtcgttaaag tatttttttg tcaattcagc agtaaaatag ggcttgttaa
     1501 aatctaaatc tgccattgct tcatttaatt gctcaattaa atcgctgttt ttttttgctg
     1561 ttgcaaaata gtagggaagc gtttctactt ttgcaataat cgtagtatta ggaatttcga
     1621 ctaagttgct gtataagagt gcatcgaatt tgccttcttg gagttctttt ataccggtat
     1681 ctgattctgt taaaacaaag tcataggaaa agttgttttt ttgtgcaaat ctttcaagac
     1741 cttcattgaa ggcattcgtt ggaaaactag caatacgaat acccttaaag gattcaaagt
     1801 cctgaaataa taagggactg tcttttttag catatagtag gctatgttca aaaccgagct
     1861 cacggtttga aaaaagatag tctttttcac ggctaggagt tttttgcatg gggccaaaaa
     1921 aatcaatact tccgtctttt aatcttttta agccttcttc ccattcgaga ggaacaaatt
     1981 catatgacca tccagtaaat tttgagagtt cagcaaaata ctctactgta tgaccttcaa
     2041 gtttatttgt ctcaccgtga aaactgatgc cataattggg caaaaagcct atttttatag
     2101 ttgtttttgg attgttttca gcgcttatta gacaaagggc acaaagcagc aagaaactta
     2161 taaaagcaca atattttttt agcatttttg agatctaccg tcctagagta tttccgatac
     2221 tatattttag catttaattt taaaaaaaac aaggtaaatt tattattcaa aactccgata
     2281 attaaaataa gaacatgtaa ttgaggtaga atatgattcg tggatggtat ataggcgcaa
     2341 gtggcatgaa tgcgcaacaa gcacgcttag atgcaatttc aaacaatctt gcaaatgttg
     2401 atacaacagc atacaaaaaa gatgttgcgg taaataaatc tttccctgag cttcttttac
     2461 gtcgactcaa tgacgatggc gtctataaaa catactttgg ttcagcagat gctgcaccga
     2521 ttataggaaa aataggactt ggcgtagaaa caaatgagct ttacaccata ctcgaacaag
     2581 gttcattcaa acaaacagga agtttcactg actttgcctt acaagaagaa ggtttttttg
     2641 ttgtagaaac gccccaaggc gagcgatata cgcgaaacgg taactttatg gttggcaaag
     2701 aaggttattt acaaacaaaa gagggctatc ctgttttagg cgataagggg cggatttacg
     2761 tagaagatat taaattcgtg gcaaaggaag acggtgtatt gtattctgaa gaagatatgg
     2821 aagaaataga ccgactaaaa cttgttcgct ttgataatga gcgctatctc aaaaaaattg
     2881 gctcaaatct ctatcaagat acaaatataa gtggcgaagc ctatagcgca gaaggttttg
     2941 agcgccccat agttttacaa tcctataccg aaacatcaaa tgtaaatgta gttaacgaaa
     3001 tggtacaaat gatcgatgta aacagggctt acgaggcaaa ccaaaaaact attcaagcag
     3061 gggacagtat gatgagcacc ttatggacga aggttgcatc aatacgttaa aaaggcgaca
     3121 aagaacgata aaaacaaaat tgtttaaaag ataaaggggt agacatggta cgaagtttat
     3181 ggacagcagc gactggaatg aacggtcagc aatttcaaat agatacaatt tcaaacaatc
     3241 tttcaaatgt gaatacaaca ggttttaaaa aacaaagagc agagtttgaa gacctcgtct
     3301 atcaaaccgt aaaaatggca ggtactcctg caacagaaga taccgtaacg cctgttggta
     3361 ttcaaatggg taatggggta aaacttgcct caacacaaag aatgtttaat caaggcagta
     3421 tgcaaaatac agaaaacgca acagaccttg cgctcatggg agaaggtttt ttccgcgtcc
     3481 aaaaatatga cggaagcttt gcttatacaa gagatggttc ttttaaaata gattcaaacg
     3541 gtcaacttgt tacaagcaat ggttttcggg ttatgccaga agttattatg ccagaaggct
     3601 ttaatataaa tagcctacaa ataagcgatg atggacgagt tacagtaaaa gtctttggta
     3661 acgatgaacc actcgatatc gcacaaattg aaatttaccg attcccaaat gaggttggac
     3721 ttgaagctga tggcgacaat ctatttttgg caacaaatgc ctcaggaacc gctatcgcaa
     3781 gccgtcccgg ctttgaaggt gctgctatta caaaacataa gtttgtagaa atgtccaatg
     3841 tttcagttgt aaacgaaatg gtacaaatga tagtcgcaca aagagcctac gagtttaact
     3901 caaaggcaat ccaaacaagc gacaccatgc tttctaccgc agtaaacctc aagcgatagg
     3961 ctctgagaat agataagtaa aaaggggaaa cataatggca gccattgact tagtatacaa
     4021 tacgacaaat ctttcacata tgggtaactt tacagcttta tctggtggac tttcttccgt
     4081 aacaaagggc gatgccattg cagaagcagc acagctttcc aaagaagttt cagactttca
     4141 aaacttactt gattccatca ctcgagaagc tgaaacaaaa gaaagcgcag tcccaagttc
     4201 ccgtctcaat ggcgattatc tgtcaagttt ttcctcagca acaaatactg ccgacacaag
     4261 ggcaaaaccc caaggcgagg cagcaaattc tgcaaatcct catattccat ctcatagcac
     4321 aaggaccatc gataaaagct cagccctcta tgaaaaatct ctagagcttg aatcctactt
     4381 tgtaaaaatt atgctctctt ctatgaaaaa tacaatcaat ctaagtgaaa taaacggcaa
     4441 agaatcttac gcctcatcca tgtacaccga tatgctctac gatgaataca atgtaatcct
     4501 cacaaaaaat gcaggctttg gcttagccga tcaaatttac ttacaactaa gcgacgataa
     4561 ttaagcgatg cctatttttt ctcagaacca tacttgagta agtctctttc ttttattata
     4621 ctttgcctat gaaagaagat aataagacag aaactgaaac tgaaacaaag ttagaaacaa
     4681 gtatatcaaa tgccacctat gaaaaggcaa aagaaaaaag cgcaaaactc gaagcagata
     4741 ttatagcaaa tccacaaaaa tatcgcgttt taactggaga ccgaccaaca gggcgcttac
     4801 acgtcggcca ttattttggc tccttacaaa accgcgtgcg cctttcaaag ctcggtgtac
     4861 ccacctgcat cctcatcgcc gattaccaag ttttaaccga tcacgatgcc tttgatgaaa
     4921 tagcacaaaa cacaaaacag cttgttatag attatatcgc atccggtatt gaaccctctg
     4981 aaaatattat tatctatcct catagctatg tgcccgaagc aaatcaattg atgctcccgt
     5041 ttttaaccct tgtcagtaat tccgaactta atcgaaatcc caccgtaaaa gaagaaatac
     5101 aagcaggtgg cttaaaaaca gtcaatgcag gtatgtacac ctatcctgtg catcaagctt
     5161 gcgatatttt attttgtaag ggcaccgtcg tccctgtagg aaaggatcaa ctcccgcacc
     5221 tcgaactcac tcgacttatt gcacgtcgat ttaacgaaaa gttttcaccc aagaagccga
     5281 tttttcccct gccagaacct ttgttaagta aggctccgac aatccttggc ttagatggca
     5341 gtcaaaaaat gtcaaaaagt cgtggcaatg ccattatgct cagcgcgaca gaagatgaaa
     5401 ccgcagccct cataaaaaaa gcaaaaaccg atgctgacag aaatatcagc tatgatccag
     5461 aaaatagacc cgaagttgca aacctactta tgatgataca tctttgcaca ggagaagcac
     5521 cagaagctat tgctgctcga attggtgatg gtggaggagg ggctttgaaa aaaatgctca
     5581 ctgaggcatt aaacgaaacg ctcagaccta tacgccaaaa acgaagcgag cttgaaaaag
     5641 atgatgcgta tattaaagaa gttttacaaa atggcatagc gagtgctcga aaaatggcca
     5701 tcgaaaccct cgatgaggta cgtcgtgcaa tgaatatgga aataaactcg ttttaaaaag
     5761 gctctttttt ataaagctct tttgcccttt gcagcaagag agctttttta tttaccgaag
     5821 gattttctgt aacacttgaa aataattcgt ttaatatttc tcctattatt tttcccttgt
     5881 agccttcttg taaaagatcc ctgccattta tagcaagatc ctggagactc aaggcggtat
     5941 tttcatctaa aaccttttct atacgttcac gaaactcacg aataacagaa aaataaagct
     6001 caccaggttt ttctcccttt gttgcccatg aatcacaaag atgcaattca aacaactttt
     6061 ccaaaggttc tactcctaca cgaacaacaa agcgacgtac cgccgcatca ctccatgcgc
     6121 tttcatagct tatcatatgg tgctgaataa gatgacagac ttcttgaatg agcttatttg
     6181 gaaaacgaag ccgttgtaaa agtgtccttg ttttttccac agaagctttt tcatgattgt
     6241 gaaaactaac aattgtactc aactttgcag gacgctttgg gtctaggctt tttaaacttt
     6301 cttttcgaac atcaggcttc ccaatatcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnac tttttttata gcatcatagg tttctggcga aatctcatag gaaagctggc
     6481 ttgaaaaacg tattgctcga atagggcgaa gcgcatcctc agtaaaacgc tcgataggat
     6541 taccaacagt ttgtatacat cggtttttaa tatctgcctg tccgttaaat ggatcgataa
     6601 tgcgaccggt ttttagattt gccgctattg catttatcgt aaaatcacgc cgcgaaagat
     6661 cttcacccaa acttgcatta aattgcactg agcttggatg gcgagaatct cggtaatctg
     6721 tctcagttcg aaaggtcgta acttcaatat gccgccccat aaaatgcacc gttaccgtac
     6781 cgtgatcgat acccgtggga ataacttttt taaaaatctt cataacatct tcgggtcttg
     6841 catttgtggc aaggtcccag tcgctcgcaa aatcgcctcg caagctatcg cgcaccgcgc
     6901 cccccacgag ataggcttca aaaccagcct cttcaaaaaa gcgattcatt ttttgtaatt
     6961 ctttaggaat agcaatcgaa gccatctata tcaatccttg tactctatcg ataaggcgaa
     7021 aaaagagaaa acatttttta tgcctctttc tactaacgca tttttttcaa agcttccagg
     7081 gcgcttgcag cgtcatcacg cgaaaccgca ataaaatcaa tcaaaatagg gtagtcttgc
     7141 aaaacctttc cccactttgc aaaacgctca atctgtaaaa aatcctcggc gcttttgctc
     7201 gcttccgttt ctgcgatatc aatcaaggat tttcgaaccg cctcttgata atgaaagtaa
     7261 gcctcttttg ccaaaagata gagttctgtg tcgggtgttt tttgactttc tatttcaaga
     7321 gagagaattt caatttcaga attttcttct gaaagttttt ttatcaaagt ctctgtatcg
     7381 ataaaagcaa gaaaatcagc acttgcctca gttttttgca aaagaaggga aacagccctt
     7441 tgatttattt ttttagcctc tttttccaaa aactgattta actcatcttg cccaagagca
     7501 tttgtccgtc tgacaaattg aggcaaagca gaatctcgca gttttatctg agagcgaata
     7561 gtaaaggcat agctaaaatc tgggcgtccc tccaaaaact cccgatatat atcggaagag
     7621 ggaaggcttc cctggatttc actttttacc tcaaggggat aaatggaaaa actacgcatt
     7681 tcggtattgg ttggtaaaag ccgctcccaa cgccagataa aagagccagc ctccagcgtt
     7741 tgagggtgaa taccacccgt tttggaaata aggaccgcag aagaacctgc aggaatcgca
     7801 agctgtaccc atccaataaa aaaaataact gccgcaaaaa tgagtaaaat gatactgctc
     7861 ataataaact ttttcataaa acaccttgta cattctatgg acgaagccat ttgattaacg
     7921 tatacttagg taagtataat acataaggac gaaatgtaaa agcattatgg caaaaaaaag
     7981 acaaaaaccc atattgtatc aaattctttt tagaataagc ctcctttttt tctttttttt
     8041 actcatcatt ttttctatgt acgtaaacgg aaactttaga aacttccaag aagagacaca
     8101 atccttttta ttgtttttga taagcatcgt ttcagctatt gtcttgtcat tgtcgcttat
     8161 ctgtgccatc gcgacccttt attttagcat acgattacga gcttttattt ttctttcaaa
     8221 tacaattttt ctgcttataa gctttagcct ttcatttctc ttattatttt tttcaagaac
     8281 aatacacctc ctctcattcg gcttttaaca gaaaaataat catgtcaaac tttgcggcat
     8341 ggatgtttga aaaaacgttt ctctgtcacg cgcaaaaaga agatagacat cttgttttcc
     8401 atggtgtaaa ataccttata cacgaggaag attatgaaga aaatacagta cgataggata
     8461 ttaaaagatt gtttttggga tttaaacctc tctgaaaatg atattaaaga aatcattcaa
     8521 ggtgatgatg aaagaaaaaa aatgatgctt tttgaaaaaa ttcttttaag tagtactgct
     8581 ttttttaaag accttgaaat atttagcaaa aatgaattaa aaaaacttct cgaaaactat
     8641 acccttcctt catttaataa agattttgca tttcgaagaa aaaatatggc tgaagtgtat
     8701 ttttttgaca aagccctttt aattagcgag ctgcaatgga tagcttaaat tataaaaaat
     8761 tatatgcact gcaagatgag gttttagacc ttgtctttgc cactgaaaaa gaattttatc
     8821 ttacaggagg aacctgttta agcaggtttt attccgagaa acggtattcc gatgatttag
     8881 atttttttac caaccaatca cctcgttata gctttgcgct tcgcaatatt aaaaaagttc
     8941 taatggaaca tttttctctt agcgttgaag tcgaagctaa agatttttca cgttataaaa
     9001 ttgactccct tctgcaaata gattttgtta acgatatctc gtttcgttat aaagaaccta
     9061 ttataaatga acagaactat cttatagaca atttagaaaa cattttaagc aataaaattt
     9121 ctgctataat tggaagagat aaccccaaag atatttttga cctttattta atatggaaaa
     9181 tttcacaggt acattgggct catatccttg aagctgcaca caataaaaca ggttttacta
     9241 atgaggaact tattataagg cttaaaacct ttccttctca gatgcttagt tcaattaaat
     9301 tgactgaacc acatttctta gataattttg aaaaagaatt tcccctactt atttctgaaa
     9361 taattgaaat tgattcaaga ggctattaac attttctgcc ctcactttgc ttggccttct
     9421 tacaagatta taggtttatc ctaacacgga tgttagaata aacctatcct tgtatttccc
     9481 tcgctctcgt gttatactaa tcctatacat caaagtaaag aagagagaca ttgtgcatat
     9541 aaatagtaga aaaagagcaa acttaccact taccacttac cacttaccac ttaccactta
     9601 ttagtatttg taccattttt tatagatttc tcagtattgg accgcgaaaa aactcgcttt
     9661 gtgagttttt ttgcggtttt ttatatcttt ttggaaaaaa catttgccta gcaagtgaaa
     9721 aaatacaaaa acacacctcg attgaggtaa aaaaggatga gaaagcatga aaaaaacatt
     9781 ttttttgcta acaggagttt tatttgtcct cctgtttttg ggctgtccaa atgcgacaga
     9841 aacaccaaaa agtaataatg caaatttgca ggctttaacg ttcagcacgg gcactttaag
     9901 tccagttttt agtccaacgg agatgaacta cacggttacg gttcccaaca gtgttacaag
     9961 cctaacagtt accgggacga agtctcatgc taacgctacg atggatctta gcgatgggaa
    10021 gctagagttt acgaatcttt cggtgggcac ttcgagtgag caaactatta aggtaacctc
    10081 agaggacggc agtgcgacaa atgaatacaa ggtgaaggtt ctgcggcagt atattgaaat
    10141 gcttcctgtc cccgcaggtt ctttccaacg cgatgaaact acaacaaata tttctaagat
    10201 tacaaattcc tattatcttg gcaagtaccc aataacacgg cagcaattct ttgatgtaat
    10261 gggaaaagat ccaagtgata cggctaagtc cagtggtatg aacgacccag tacaaatggt
    10321 aaactggtac catgcaatag ccttttgcaa taagttgagc ttgttggaag gtttaagtcc
    10381 ggcatatacg gtaggtgggg ttagcgattg ggcagcttta gcctttagct ctattcctac
    10441 aagtgaaaac aaaaattggg ataaagccga atgcgattgg gaggcaaacg gttaccgcct
    10501 acctacaaaa aaggaatgga tgtggg
//
LOCUS       DAOSSW010000062        10292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_6338_length_10292_cov_7.420436, whole genome shotgun sequence.
ACCESSION   DAOSSW010000062 DAOSSW010000000
VERSION     DAOSSW010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10292
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6338_length_10292_cov_7.420436"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1285)
                     /locus_tag="PKW26_06100"
     CDS             complement(77..1285)
                     /locus_tag="PKW26_06100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ93174.1"
                     /translation="MKIAFFADCFFPQLNGVVTATLMLAEELHKRGHEVHILAPSYRK
                     YKGDNQFPFVVKRFASFNARFYDDFKWVFNPNPSLLRYFRKHNFDILHFQAPMSMAAQ
                     AIFIAKVYKKPLIGTFHTFFADPEYLKHAKLNYPIVEKFGWYYSNKHYNRCDVVTVPS
                     KQTGIELKNNKCTAPIRYISNGINLKQFDNTKAAEFRKKWNLSEKDIVFLFVGRIAHE
                     KNILSLLKVFSHIGEKNKAIKLLIVGGGPQKDTYASYVKEHSLQNNIQFTGSINHDDL
                     VVSGVFGACDYFITLSKTENQPITILEAQANGLIPFCYAEKGLKTMIEHGKTGWFLEN
                     KEENLCGDELLSIISNTEQINNLKETMLAYIQSQSIENIAVEWENLMQELIEQKKTKK
                     KFKSQEKKVV"
     gene            complement(1361..>1702)
                     /locus_tag="PKW26_06105"
     CDS             complement(1361..>1702)
                     /locus_tag="PKW26_06105"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOQ93175.1"
                     /translation="ILRKAKKQRVKKDLLSKLFGAMMPRGSKKLGLSKMNMAGMGPKM
                     IRSVMKKKNIASLEELIAQAQSLGVELQACSMSMDVMGIQQVELIDGVKLAGAAAMIA
                     NAEESDMSLFI"
     assembly_gap    1703..1802
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1803..3796)
                     /locus_tag="PKW26_06110"
     CDS             complement(<1803..3796)
                     /locus_tag="PKW26_06110"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ93176.1"
                     /translation="MNNQKKILIVGGVAGGASAAARLRRLDENAQIIMFERGQYISFA
                     NCGLPYYIGGEIKQKSALTLQTPESFNARFNVDVRVMQEVVAINPKKKTVSVHNLVTN
                     SKYEETYDDLILSPGAEAVRPPIPGLNSKKVFTLRNIPDTYAIKDYITEKNARRAVIA
                     GGGFIGIELAENLRFAGLEVSLVEMADQVLAPIDYDMAVEVHKHIEQKGVQLELKAML
                     KSVQETKDGLQLTLQKDGTTEEILETDFLVMAIGVRPESKLAKDAGITLNEKGAIVVD
                     ASMKTSEPNIYAVGDVIEVQDFVTQTKTFVPLAGPANKQGRIAADNICGIQSTYKDTQ
                     GTSILKVFDLTVASTGINEKTAKAKNIRYDKIFTYSANHASYYPGSLSMAIKTLYDPK
                     DGKILGAQIVGYEGSDKRNDVLATAIRAGMTASDLKDLELSYAPPFGSAKDPVNMIGY
                     AIDNILTGKVKQIHHHDIENLPKDGSVQLIDTRTVEEFETGTIPGFINIPLDDLRSRI
                     GELDKSKKTYVTCRVGLRGYIAARILSQNGFDVYNLSGGYRQYNMVYGKTLPVEPTNF
                     NIETQMMESASPQKEGIFSYGQAPENKLEETRMQASSGKLIQIDACGLQCPGPIVTLS
                     GSIKNANPGDIVEISVTDPAFAGDVEGFCRRTGNTLLGMTT"
     assembly_gap    3832..3931
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3932..4973)
                     /locus_tag="PKW26_06115"
     CDS             complement(<3932..4973)
                     /locus_tag="PKW26_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOQ93177.1"
                     /translation="MLELLIILGALIVFLLLIILIKLFTAKAETPELGELRKQLEELK
                     TKQLEAQNTSLRQQQNLFISSQKEMRESLQYIMKSVSDSLTNSQGHIHNQLKNSNEVI
                     GEIQQKLGSLETTTKNIQDISKDISSLQEILSAPKLRGNLGEYLLEELLRDILPPQHY
                     AMQYRFKNGTTVDAIIRLQGGIVPIDSKFPLESFQRLIKAETNEEKKSYKKEFISSVK
                     KRIDEIASRYIIPSENTFDFAMMYIPAENVFYETIINDGLTNKDYEIFNYAIQRHIIP
                     VSPQSFYAYLMAIAYGLKGFRIEQEAKHIIGELSRVQDSFARFLKDFSLLGSHIGNAE
                     SKYADLNQRASKM"
     gene            complement(4973..5881)
                     /gene="rocF"
                     /locus_tag="PKW26_06120"
     CDS             complement(4973..5881)
                     /gene="rocF"
                     /locus_tag="PKW26_06120"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HOQ93178.1"
                     /translation="MSIHILEMPLDFGGNRHGSDMGPSAIRLAGLKDRLQKLGLETKK
                     YFSPIELTPQEYEDEGEENARFLKPIVMACNQLALQVETALKQNAFPLVLGGDHSISL
                     GTLAGLSSYCREKDCSFGVLYVDAHGDFNTTETTPSGNIHGMCMAASCGYGIPELTNL
                     YENGRKVDPKNVCYVGVRDIDPGEKLLMKEAGVTVFTMSDIDRQGFSSILKKILVFFK
                     SHVDFVHISFDMDVIDPMFAPGVGIPLPGGLNYREALLLMEEMHHLGLVRSAEFVEVN
                     PVLDVRNQTATMACELIARLLGHEIY"
     gene            6042..7076
                     /locus_tag="PKW26_06125"
     CDS             6042..7076
                     /locus_tag="PKW26_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOQ93179.1"
                     /translation="MNKTIVCFGEIMLRLKSPGFERLLQSPLLEATYGGGEANVAVSL
                     AYFGKKTAYVTALPDNALGDGALRELQKHGIDVSNCIRSCGRMGLYFLEGGSMQRAST
                     VLYDREGSSIARACAQDFDWKAIFQNASWLHLTGITPAISQAAADSSLEAVKQAKKAG
                     LKVSIDLNYRKKLWNYGKTAPEVMTELVKHADLLIANEEDIQKSLGMTSPVQVESGQL
                     DIDSYKTLLTEVREKFPQLEWIAVSLRESFSADENNWSGLLSTKSTLYQSKKYELRDI
                     VDRVGGGDAFSAGLIKSLIEKPEDPQAALDFALAASALKHTISGDFNLVTEAEVKSLL
                     QGDSSGRVQR"
     gene            7121..9628
                     /locus_tag="PKW26_06130"
     CDS             7121..9628
                     /locus_tag="PKW26_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOQ93180.1"
                     /translation="MKVSQKLGMSIGFFFLLMAALLLVSLGLSFLYYDTSSVTQNFFA
                     YIGLALVSIYGYSSLLVPVFLFVSGIVLFLPPWNAKKAFLLAFSLLPFFTVVFAEKLI
                     KTMTQESSQAIMRLQIFGIFVVAIIMLIIEYLSANLVFDAAIEILTAKKNPKIKETKA
                     QENLGQKNLQKEINPDALKIEETKDLLSELDSFLPPEEDPALFEEIKNTEILEDIAQG
                     EEAFFDDVSPTSLADNALFFSELETVDEIALDETTPDLNLNQKSQQDAEVEIRAEKKD
                     APVLLRFDKPKIDILEIAKEKSTNFESEDEEAFLLNPFGDDDFDLDDADIDIDIDDDI
                     DDSDDELEIEIEDIDFEEKSVVKADSKKKLKAYELPADELLTSYPGDEYWVIGKETEE
                     AAQLLKETINEFKIHAEVTGIRKGPVITMFEVLPAPGVKLSKIVSLQDNIALRLAASS
                     VRIVAPIPGKSAVGIEVPNKERFIVGFKELIDTEHVAYKKMDIPVVLGKDISGEAQFL
                     DLTKTPHLLIAGATGSGKSVCVNSMILSILFKRSHHDVKMILIDPKVVELKLYNDIPH
                     LLTPVITEPKKAFQALQYCLCEMERRYALLDGMGVRDIKNYNKRIVERSIATEKLPYI
                     VIIIDEFADLMATTGKELESTVARLAAMSRAVGIHLVLATQRPSIDVITGLIKANIPS
                     RIAFMVASKTDSRIIIDQVGAEKLLGKGDMLYASTTDPFPVRIQGTFVSDSEVETVVE
                     YVKKIAPPDYIDDEIFFEDDDDFELQGDYTSDGLDPLYDKALEIVVQAGKASASYIQR
                     RLKIGYNRAARIVEDMEARGIVGPQNGSKPREIIHVP"
     gene            complement(9678..10253)
                     /locus_tag="PKW26_06135"
     CDS             complement(9678..10253)
                     /locus_tag="PKW26_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93181.1"
                     /translation="MKQSKIIIALILFVSIVGGASAIEFNWQNYADCVKQGDILVNAG
                     IGFGTPVAGDMVIPPIQASVEYVLPIAGFPTSFGGLVGFTTSALDTVYSKYDYTGIAF
                     GGRASWHFDLGIDKLDTYSSLALGYFKYSMKDTPKAGYEDWPKLETDYSQFYYGFNIG
                     ARYFFTPVIGASLEIGYSALSYVAAGISLKF"
BASE COUNT         2858 a         1901 c         2139 g         3194 t
ORIGIN      
        1 tgctattata gaagcatggt acacaaaaaa gatataggtg tttatctcgc cgtaagtatt
       61 tccttttttt tatttcctag acaacttttt tttcttgact tttaaacttt ttttttgttt
      121 ttttttgctc tatcaattct tgcatgagat tttcccattc tacagcaatg ttttctatag
      181 attgactttg gatgtatgca agcatggttt cttttaagtt atttatttgt tctgtatttg
      241 atataataga aagaagttca tcgccgcaaa ggttttcctc tttattttcc aaaaaccatc
      301 ctgttttacc atgttctatc attgttttta aacccttttc agcataacaa aaaggaatga
      361 gcccgtttgc ttgtgcttca aggatggtaa taggctggtt ttcagttttt gaaagtgtaa
      421 taaaataatc acaggcacca aaaacgcctg atacaacaag gtcatcgtgg ttaatgcttc
      481 ctgtaaactg tatgttgttt tgaagtgaat gttctttgac gtagctagca taggtgtctt
      541 tttgtggtcc gccgccaaca atgagtaatt ttatagcttt atttttttct cctatatgag
      601 aaaaaacctt aagaagagac aaaatatttt tttcatgcgc aattcttcct acaaataaaa
      661 aaacaatatc tttttcagat aaattccatt tttttcgaaa ttctgctgcc tttgtattat
      721 caaattgttt tagattaata ccgtttgaaa tataacgaat gggggcagta catttattgt
      781 tttttaattc aatgcctgtt tgttttgatg gtacagtaac aacatcgcaa cggttataat
      841 gtttgttaga ataataccag ccaaattttt caacaatggg ataattaagt tttgcgtgtt
      901 ttaagtattc gggatctgca aaaaaggtat gaaaggtccc aatgagcggt tttttatata
      961 cctttgctat gaagatagct tgtgcagcca tagacattgg tgcttgaaaa tgtaaaatat
     1021 caaaattgtg ttttcgaaaa taacgtaaaa gactggggtt tggattaaat acccacttaa
     1081 aatcatcgta aaaacgggcg ttaaaagatg caaaacgctt tactacgaag ggaaattggt
     1141 tgtcgccctt gtacttgcgg tatgatgggg ctaaaatatg tacttcatgc cccctcttat
     1201 gaagctcttc tgctaacatg agcgttgcag ttaccactcc atttaactga gggaaaaaac
     1261 aatctgcaaa gaaagcaatt ttcatagcat aagcctatca aaaacaggct tttaaaacaa
     1321 gggatatttt tgatagggct tcgtattgct ttgtatgctt ctaaataaac aaagacatat
     1381 ctgattcttc tgcatttgct atcatggctg cagctccagc tagttttacg ccgtcgatca
     1441 gttcgacttg ttgtatcccc ataacgtcca tgctcataga gcaggcttgg agttcaacgc
     1501 cgaggctttg tgcttgggca atgagctctt ctaagcttgc aatgtttttc tttttcatta
     1561 cggagcgtat catcttggga cccatgccag ccatattcat ttttgagagg cccagttttt
     1621 tacttcctcg tggcatcatt gctccaaaga gttttgaaag taaatctttt ttgacgcgct
     1681 gtttttttgc cttacgcaag atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nngttgtcat gccaaggagg gtatttccgg ttctgcggca aaagccttcg acgtctcctg
     1861 caaatgcggg atcggttacc gaaatttcaa cgatatctcc agggtttgca ttttttatcg
     1921 aacctgaaag tgttacaatg ggaccgggac attgcaaacc gcaagcatcg atttgtatga
     1981 gttttccaga gcttgcttgc atccttgttt cttcgagctt gttttctggc gcttgcccgt
     2041 agctgaaaat accttctttt tgcggagacg cactttccat catctgtgtt tcgatattaa
     2101 aatttgttgg ctcgacagga agcgtttttc catagaccat attgtattgg cgatagcctc
     2161 cgctgagatt atacacgtca aatccatttt gacttaaaat tcgtgcagcg atatagccac
     2221 gtaaaccaac acggcatgtt acataggttt tttttgattt gtcgagttcg cctattctag
     2281 agcggaggtc atcgaggggt atgttgataa agccgggaat agtgcctgtt tcaaattctt
     2341 ctactgttcg tgtatcgatg agctgtacgc ttccatcctt gggaaggttt tcaatgtcgt
     2401 gatggtgaat ttgttttacc tttcctgtca aaatattgtc aatggcatag ccgatcatgt
     2461 tgacggggtc ttttgcagag ccaaagggtg gcgcatagga aagctctaaa tcttttaagt
     2521 cgcttgctgt catgcccgct cgaattgccg ttgcaagaac atcgtttctt ttatcacttc
     2581 cttcgtagcc gacaatttgc gctccaagga tttttccatc ttttggatcg tagagggttt
     2641 ttatagccat gctcaaggaa cctggataat agcttgcgtg atttgcagaa taggtaaaaa
     2701 ttttgtcata gcggatattt tttgcctttg cggttttttc gtttatccca gttgaggcaa
     2761 cagtgaggtc aaaaaccttg aggatagaag tcccttgcgt gtctttgtag gtgctctgaa
     2821 ttccgcaaat attgtctgct gcgattcgcc cttgtttatt tgcaggtcct gcaagtggga
     2881 cgaaggtttt tgtctgtgtt acaaaatcct gcacttcgat gacatctccc actgcgtaaa
     2941 tatttggctc ggatgttttc atagatgcat cgacaacgat agcacctttt tcatttagcg
     3001 taatgcctgc atcttttgca agcttggact caggccgcac tcctatagcc ataaccaaaa
     3061 aatctgtttc aagtatttct tccgtagtgc catctttttg taaggttaac tgtaaaccat
     3121 ctttcgtttc ttgcactgat ttaagcatcg ctttgagttc aagctgtacg cctttttgct
     3181 caatgtgttt gtgaacttct actgccatgt cgtagtcgat gggggcgagc acttgatcag
     3241 ccatttcgac aaggcttact tccaggcctg caaaacggag attttctgca agctctatcc
     3301 cgataaagcc tccaccggca ataaccgctc tgcgagcatt tttttcagta atgtaatctt
     3361 tgattgcgta tgtgtcagga atatttcgaa gtgtaaacac tttttttgaa ttaagcccag
     3421 ggatgggagg acgaacagcc tctgcgccag gtgataaaat aagatcgtca tacgtttctt
     3481 cgtattttga atttgttaca aggttgtgca cagaaaccgt ttttttcttt gggttgatag
     3541 cgacaacttc ttgcataaca cgaacgtcga cattgaatcg agcgttgaaa ctttctggcg
     3601 tttgcaaggt gagtgctgat ttttgtttta tttcaccacc gatataatag ggcaagccac
     3661 agtttgcaaa agaaatatat tgtcctcttt caaacataat gatttgtgca ttttcatcaa
     3721 gtcgacgtaa tcgagcagcg gcagatgcac caccagcaac tccacctacg ataagaattt
     3781 ttttttgatt attcatagaa tccccttccg tcataaaggc ggtacaaaaa annnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcattttgc ttgccctttg gttgaggtca
     3961 gcatattttg actcagcatt tccaatgtgg cttccaagaa ggctaaagtc tttcaaaaac
     4021 cgtgcaaagg agtcctgtac acgagaaagt tccccgatga tatgttttgc ttcttgttca
     4081 atgcgaaagc ccttaagtcc ataagcgatg gccatgagat aggcgtaaaa actttgtggc
     4141 gagacaggta taatatgccg ttgaatagcg tagttaaaaa tctcgtagtc tttgtttgta
     4201 agcccgtcgt ttataatggt ttcgtaaaaa acattttctg cagggatata catcatcgca
     4261 aagtcgaagg tgttttcact tggaataata taacgacttg caatttcgtc aatgcgtttt
     4321 tttaccgaag aaataaattc ttttttatag cttttttttt cttcatttgt ctcagcttta
     4381 atgagccttt gaaaactctc caagggaaat tttgaatcaa taggaacgat gccaccttgt
     4441 aatctaatga tagcatctac cgtggttcca tttttaaaac gatactgcat agcatagtgt
     4501 tgcggcggta agatatcgcg taaaagttct tcgagaagat attctcccag atttccccga
     4561 agttttggtg cgcttaaaat ttcttgcagg gaagaaatat ccttacttat atcttggata
     4621 tttttagttg ttgtttcaag gcttccaagt ttttgttgaa tttccccaat aacttcattt
     4681 gagtttttta attgattgtg tatgtggcct tggctatttg taagggaatc gcttaccgac
     4741 ttcataatgt attgcaagga ttcacgcatt tctttttgtg aagaaataaa aaggttttgt
     4801 tgttgtctga gcgaagtatt ttgtgcttca agctgttttg ttttaagttc ttcgagttgt
     4861 ttgcgtagct caccgagttc tggtgtctct gcctttgcgg taaatagttt tatgagaata
     4921 atgagcaaca aaaaaacaat aagggcacca aggataatca ataattcaag cattagtata
     4981 tctcatggcc caaaagtcgg gcgataagtt cgcaagccat cgtagcggtt tgattgcgca
     5041 catcgagaac gggatttact tcgacaaatt ctgcggagcg aacgaggcca aggtgatgca
     5101 tttcttccat taaaagaagg gcttccctgt agttgagacc gccgggaaga gggatgccga
     5161 cgccaggtgc aaacatgggg tcgataacgt ccatatcaaa actaatatgc acaaagtcga
     5221 cgtgagattt aaagaataca aggatttttt ttagaatgct tgaaaagcct tgcctgtcga
     5281 tatcgctcat ggtaaagaca gttacgccgg cttccttcat aaggagtttt tctcctgggt
     5341 ctatgtcgcg cactccgaca tagcagacat tttttgggtc aacttttcgg ccattttcat
     5401 acaagtttgt cagttctgga atgccgtagc cgcaagaggc tgccatgcac atgccatgaa
     5461 tgtttcccga gggtgtggtt tcggttgtgt taaaatctcc gtgggcatcg acgtaaagga
     5521 caccaaaaga gcagtctttt tctctacaat acgaagaaag tcctgcgagg gtgccgagtg
     5581 atattgagtg atcgccgccg agaacgaggg gaaaggcgtt ttgtttaagg gctgtttcaa
     5641 cttggagagc aagctggtta caagccataa caatgggctt tagaaaacgc gcattttctt
     5701 cgccttcatc ttcgtattct tgtggagtga gttctatggg ggaaaaatat ttttttgttt
     5761 caagaccgag tttttgcaac ctgtctttta atccagctaa acggatagca cttggtccca
     5821 tgtcagaacc atgcctattg cctccaaagt cgaggggcat ttccaaaata tgtatgctca
     5881 tcttttcatt ataaggtggt ttttttagca tgacaagagc gcctagggaa atttttgttt
     5941 tcaagcaaaa aaggttaaaa aaacagagcg atttccttgc ctttttttct ctttgctatg
     6001 ttatactagt agtatataat ctgaaactat gaggaattgt gatgaataaa accattgttt
     6061 gttttggaga gataatgctt cgcctaaaat cgccaggttt tgaaagactt ttacaaagtc
     6121 ccttgcttga ggcgacctac ggtggcggcg aggcaaatgt tgcagtaagc cttgcatact
     6181 ttgggaaaaa aaccgcctac gtaacagccc ttcccgataa tgcgcttgga gacggagcgc
     6241 ttcgtgagct acaaaaacac ggcattgatg tatcaaactg tattcgaagt tgcggacgaa
     6301 tgggcttgta ctttttagaa ggcggttcca tgcaaagagc ttcgacggtg ctttacgaca
     6361 gagaaggaag cagcatcgca agagcttgcg cccaagactt tgactggaag gctattttcc
     6421 aaaatgcgtc gtggttacat cttacgggca ttacgcctgc tatttctcaa gccgctgccg
     6481 attctagctt ggaagcggta aaacaggcaa aaaaagcggg cttaaaggtt tcgattgact
     6541 taaattatcg aaaaaagctc tggaactatg gaaaaactgc tccagaagtg atgacggagc
     6601 ttgtaaaaca tgcagattta ttgattgcaa atgaagaaga tatacaaaaa tcacttggga
     6661 tgacttcgcc tgttcaggta gaatctgggc agctcgatat agattcgtat aaaacacttc
     6721 ttacagaggt tcgagaaaaa tttccacagc ttgaatggat tgcggtaagc ttgagggaat
     6781 ctttttcagc cgatgaaaat aattggtcgg gcttgttatc gactaaatcg accttatatc
     6841 aatctaaaaa atacgaattg cgcgatattg ttgacagagt tgggggagga gatgcttttt
     6901 ctgctggcct tatcaagtcc ttgatagaaa aaccagaaga cccccaagca gccttggatt
     6961 ttgctcttgc ggcttctgct ttaaaacata cgatttcggg ggattttaat cttgtaacag
     7021 aggcagaagt aaaaagtctt ttgcaaggtg atagctcagg gcgagtgcaa agataatttt
     7081 tatttcttaa tctatttttt ttcatgccga tactctaggt atgaaagttt cccaaaaatt
     7141 aggcatgagt attggatttt ttttcctcct tatggcagcc ctgctcctcg tgagcttggg
     7201 cttgtcattt ctgtattatg atacaagttc ggtaacgcaa aacttttttg catatattgg
     7261 tttagctctt gtttctatct atggctattc gtccttgctt gttcccgttt ttttgtttgt
     7321 ttcgggtatt gttttgtttt tgccaccatg gaatgcaaaa aaagccttct tgttggcttt
     7381 ttcattattg ccttttttta cagtggtttt tgcggaaaag ctaataaaaa caatgactca
     7441 agaaagtagc caagcaatta tgcgtttgca aatttttggc atttttgtcg ttgccattat
     7501 tatgcttata atcgagtatc tctctgcaaa tcttgttttt gatgcagcaa ttgaaatctt
     7561 gacagctaaa aaaaatccaa aaataaaaga aactaaagcg caagaaaacc tcggtcaaaa
     7621 aaatcttcaa aaagaaataa atcctgatgc actaaaaatc gaggaaacaa aagacctcct
     7681 ttctgagttg gattcttttt tacctcccga ggaagaccct gccttatttg aagagataaa
     7741 aaacacagaa attcttgaag acattgcaca aggtgaagaa gcgttttttg atgacgtgag
     7801 ccctacttct ctagctgata atgccttgtt tttttctgag cttgaaacag tcgatgaaat
     7861 tgccttagat gaaacaactc ctgatttaaa tctaaatcaa aaaagtcagc aagatgcaga
     7921 agttgaaata agagcagaaa aaaaagatgc acccgttctc ttacgttttg ataaacccaa
     7981 aatcgatatt ttagaaattg caaaagagaa aagcacgaat tttgaaagtg aagatgaaga
     8041 agcttttttg ctcaatccct ttggagacga tgactttgat ttagatgatg ctgacattga
     8101 tattgacata gatgatgata ttgatgatag cgacgatgag ttagaaatag aaatagaaga
     8161 tatagatttt gaagaaaaat cagttgtcaa agctgattca aaaaaaaaat taaaagccta
     8221 tgagctaccc gcagatgaac tcttaacaag ctatccgggg gatgagtact gggttattgg
     8281 gaaggaaaca gaagaggcgg cgcagctact aaaagaaacg ataaatgagt ttaaaattca
     8341 cgcagaggtt acgggaatac gaaagggtcc tgttataaca atgtttgaag tgttgccggc
     8401 accaggggta aagttgagca agattgtttc cttacaagac aatattgcac ttcgccttgc
     8461 ggcttcaagt gtgcgtattg tggcacccat ccctggaaaa tctgcggtgg gcattgaggt
     8521 tcctaacaaa gagcgcttca ttgttggttt taaagagctt atagacacag aacatgtcgc
     8581 ctataaaaaa atggacattc ctgtcgttct tggaaaggat attagtgggg aagcacaatt
     8641 ccttgattta acaaagacac cgcatttgct tatagctggg gcgacgggtt ctggaaaatc
     8701 tgtttgtgta aactcgatga tattgtcgat tttatttaaa cgaagtcatc atgatgtaaa
     8761 gatgatttta atcgatccaa aggtggttga gcttaaatta tataacgata ttccacattt
     8821 acttacgcct gttatcactg aacccaaaaa agcctttcaa gccttgcagt attgtctctg
     8881 tgaaatggaa agacgttatg ccttgctcga tggtatgggt gttcgcgata ttaaaaatta
     8941 taataaacga attgtagaaa ggagtattgc gacagaaaaa ttgccctata ttgttattat
     9001 tatcgatgag tttgctgacc ttatggcaac gacggggaag gagcttgagt caacagttgc
     9061 gcgactggca gcgatgagta gggcggtagg gatacatctt gttcttgcaa cacaacgtcc
     9121 ttcgattgat gtgatcaccg gtttaataaa agcaaatatc ccttcgcgaa tcgcctttat
     9181 ggttgcctca aaaactgata gccgtattat tatcgatcaa gtgggagcag aaaaactgct
     9241 cggcaagggc gatatgctgt acgcatctac gaccgacccc tttcctgtgc gcattcaagg
     9301 gacctttgtt tccgactctg aagttgaaac tgttgttgaa tatgttaaaa aaattgcacc
     9361 gccagattat attgacgatg aaatattttt tgaagatgac gatgactttg aactgcaagg
     9421 agattatacg agtgatggct tagatcccct gtatgacaag gctttggaaa tagttgttca
     9481 agcaggaaag gcctcagctt catatattca aagacggctg aaaatcggct ataatcgcgc
     9541 cgcacgaatt gtagaagaca tggaagcacg tggcattgtc ggcccacaaa acggctcaaa
     9601 accacgtgaa attatccatg taccctaaag ggaaaaaggc ggaagattgt ccgcctgcaa
     9661 atcctttttc tcgtagttta gaatttaaga cttatacctg cagcaacata agaaagagcg
     9721 ctatatccta tttcaagact tgccccaata acgggagtga agaagtaacg tgctccaata
     9781 ttaaaaccgt aatagaattg gctataatct gtttctagtt ttggccaatc ttcataacct
     9841 gcttttggag tatctttcat tgaatactta aaataaccaa gggcgaggga agagtaggtg
     9901 tccaacttgt cgatgcctaa gtcaaagtgc caggatgcac ggccgccaaa ggctattccg
     9961 gtgtaatcat attttgaata tacggtatca agtgccgaag ttgtaaaacc aacaagaccg
    10021 ccaaaagagg ttggaaaacc agctatggga agaacatatt caacacttgc ttgaataggt
    10081 gggataacca tgtctcccgc gactggtgtt ccaaaaccaa ttcctgcgtt tacgaggata
    10141 tctccttgct ttacgcaatc cgcataattt tgccaattaa actctattgc agatgcgcca
    10201 cctacaatgc ttacaaacaa tatcaaagct atgataatct tgctttgttt cataaaaatg
    10261 ctccttttgc gggtaacccg cccttattct aa
//
LOCUS       DAOSSW010000063        10135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_6438_length_10135_cov_7.046131, whole genome shotgun sequence.
ACCESSION   DAOSSW010000063 DAOSSW010000000
VERSION     DAOSSW010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 10135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10135
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6438_length_10135_cov_7.046131"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..990)
                     /locus_tag="PKW26_06140"
     CDS             complement(49..990)
                     /locus_tag="PKW26_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOQ93182.1"
                     /translation="MAKLGLGKGIDAVFGDNAFSPTDVTQDISQKDAREKGLPLEGQR
                     VLSEGETILTEMEVGLLLPNPYQPRKDFDQDALEELASSIKEHGIVEPIIVSQSNDGN
                     FYIVGGERRVRAAKIAGLERVPVCIKQYTKEAMLEIAIIENIQREDLNALEEAIAYND
                     LMLMSNATQEEVAKRVGKSRSAVANALRLLKLPEDMKNALSQGKISAGHARALLSVLN
                     SQEQDILFARIVEAELSVREAEQMAASLNGKGIVTKKKERARVEEQNPELAYLEEQFR
                     EKLGTKVQLKGSLEKGSLEISYFSQEDLDHLFSLLCN"
     gene            complement(983..1738)
                     /locus_tag="PKW26_06145"
     CDS             complement(983..1738)
                     /locus_tag="PKW26_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ93183.1"
                     /translation="MSKVCVFVNQKGGVGKTTSVINLGAYLAEAGKKVLLVDFDSQAN
                     MSSGVGISKEKPTVYELIAELATANDVIKQTKQKNLEAIPASIDLAGAAIELVDQDDR
                     DYYLKNAIEVLKKDYDYILIDCPPSLGILTLNGLACANEVFIPMQCEYFALEGISLLL
                     QTVKKVQQSINPSLKIGGIFFTMYDSRTRLAQDVVMQVKAYFKDLVFSTIIPRNVRLS
                     EAPSHGLPINLYDANCVGARSYKELAKEVLARG"
     gene            1799..2146
                     /locus_tag="PKW26_06150"
     CDS             1799..2146
                     /locus_tag="PKW26_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOQ93184.1"
                     /translation="MFTIYDLIQNLPAYVVHADEDSLHSEITAIVAADLMSDVLVSVD
                     APCVLVTSLASEQVIRTCDISGIQTILLVNDKLPSPAMKALAEEIGITLLATPLPMYE
                     ACVNIHLLKTFAD"
     gene            2152..3384
                     /locus_tag="PKW26_06155"
     CDS             2152..3384
                     /locus_tag="PKW26_06155"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOQ93185.1"
                     /translation="MKANLKTILALTIKASLSKPTKKSELNKVKIKLITKEPHLFYIE
                     EYTKTQSFHKTLNYDECLTYVKKILGKTFKAALFQTAEKDYQFLTNKRGFTRLLEQEP
                     SKEKNETALEKTSHNKEKNYLLAEDRPIPFLIALGIMNKEGKVLAKKQKKFRQINRFL
                     EFVNDILDELCKNTEPTEKNPLSIVDFGCGKSYLTFALYHFIVVEKKIPAKIIGLDLR
                     SDVIQSCTELAKTCKYTDLLFFNDSIENFLSDKNNSQKNLDLVISLHACNTATDYALA
                     SAVQKNAKVILSVPCCQHELHTALQKRPLPSDNPYEPFGRYGIIREQFSALTTDLMRA
                     LLLEEAGYKVQVLEFIDIEHTAKNLLIRAVKRKTGSLKDKTKNSPTPSKSTESLKILQ
                     KELGIRLKLETLLADEKP"
     gene            3493..3720
                     /locus_tag="PKW26_06160"
     CDS             3493..3720
                     /locus_tag="PKW26_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOQ93186.1"
                     /translation="MLETRVKEILEMVRPQLQADGGDLEFVALEDGGKVHVKLTGACG
                     SCPMATMTLKQGVERVLKENIPEVTEVIQAF"
     gene            4060..4617
                     /locus_tag="PKW26_06165"
     CDS             4060..4617
                     /locus_tag="PKW26_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ93187.1"
                     /translation="MKVSKHKVVRFHYSIKNSSGEILDSTFGEAPLEYVHGVDAIMEG
                     LEKALEGKEVNSSFEVLVPADDAYGPKVDELIFKVARSQFPPDVELEVGDIFNTSEQD
                     YEVTIIEVNDDEIVIDANHPLAGEDLSFDINILNIRDANESEIKNGLLGFIDDEDYED
                     GCDCGDEDCDCDCGDEDCDCSDGDC"
     gene            complement(4614..5648)
                     /locus_tag="PKW26_06170"
     CDS             complement(4614..5648)
                     /locus_tag="PKW26_06170"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOQ93188.1"
                     /translation="MNQSKDEKKENPKKFIEPKLVLDTKHDNTALILFLGSLCFFLAA
                     VENSIPKPLPFMRLGLANAPIIFALFLLNTREYFLLVFIKVLGQALISGTLFSYIILF
                     SAGGSFASALAMLFLQRLAKNQVSCLGISLFGSLANTLVQYFLARYILFGSGAVLIAP
                     LLILSGTITGIGLGVFSELFLKRSQWFVSIKEGRKAKIYSRQGQDFQRPKQKKKGFRI
                     KAFLTPLFIFCSIVFFNLFQAYGKVLFTVVGFEVTSGALYSGIKRASILTGMVFVSKL
                     IVSSSFRLPGNWGQRLDQIFYYVAMLTEKTQSDESQKKTEQSRDKKSKWDGFISVLEA
                     IDRRLVEVYF"
     gene            complement(5700..6446)
                     /locus_tag="PKW26_06175"
     CDS             complement(5700..6446)
                     /locus_tag="PKW26_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ93189.1"
                     /translation="MKKIKTIHSLKILLVVFGLALVHVLCAEENVDLTIQPNPVVQEN
                     VQRTVQNFGPVKLDALELYREGRDLEAAQRYADAQKKYQKSAEVCREEIKENSRNIES
                     YVVLGWSLIRLEQYSEAIKISNTALAISPKENRITETLAEAYFYLDNYTESLKLFEQY
                     VNALPNGDRASTAYFFIGDIYRLKGKYEHADIAYSMAVRKSPGIALWWYRLGMVREMA
                     GKKEEAKIAYGKALEYRPIYQEASDALRRL"
     gene            complement(6469..8061)
                     /gene="gltA"
                     /locus_tag="PKW26_06180"
     CDS             complement(6469..8061)
                     /gene="gltA"
                     /locus_tag="PKW26_06180"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOQ93190.1"
                     /translation="MKDTRKKSADSDASQVVDSVANCEAVSGPNYTDNYTNIDALEKK
                     ALDFIDSVDDAIKNQSFSPKERLAIPLQEMPTRNPLERSRQMCEVAIGFTESMAILEA
                     NRCLQCKNQPCVAACPVHVPIPQFIAKAAQGAFKEAVDIIKTTNLLPAVCGRVCPQEL
                     QCQSVCTVGKSLKDIDKAVGIGRLERFVADWERERNLITIPEVKPETGKKVAVVGSGP
                     AGLTLAADVRREGHSVTIFEAFHKMGGVMVYGIPEFRLPKAIVAKEIEMLKEMGVQFR
                     TNYLVGRTGTLGQLLKEEGFDAAFIGSGAGLPKFLNIPGENLIGVFSANEYLTRANLM
                     KAYKEFAATPFYQAGHVVVVGGGNVAMDAARMALRLGAKKVSLVYRRTRDEMPARKEE
                     VDHAEEEGVVFRFLENPTRILGDEDGRVVSIEVLSYELGEADESGRRSPVPIEDSEHL
                     IDCDAVIIALGNNSNPLMAQTTPGLDVDLRGQIIADAEGKTSLPAVWAGGDIVLGAAT
                     VILAMGEGRKAAASMNTYLASI"
     gene            complement(8068..8931)
                     /locus_tag="PKW26_06185"
     CDS             complement(8068..8931)
                     /locus_tag="PKW26_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOQ93191.1"
                     /translation="MHKILEKKQLSADVFYFKVDASDIVRNRKAGQFVLVQIDTEFGE
                     RIPLTIADASKEEGWIVLVFQAVGASTLKLSQKNKGDFIPAILGPLGKPTKIEKMGTV
                     VCVGGGIGVAPLYPIVQAHKAVGNKVIVIMGARTKDLLIFEDEMRSLADELFVVTDDG
                     SAGKKALVTEPLKEICERNEKPQEVVAIGPPIMMKFCSETTRPFAIHTLVSLNTIMID
                     GTGMCGGCRVTVGGETKFVCVDGPEFDGHLVDFDNMILRMKSYKKQESEDMHICRMEA
                     AAEVLDKKART"
     gene            complement(9021..9332)
                     /locus_tag="PKW26_06190"
     CDS             complement(9021..9332)
                     /locus_tag="PKW26_06190"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93192.1"
                     /translation="MNIVLVGAGSVQFGNWIAVEVPAIINKDGVQGINLGELPKGYAS
                     LLNAYCGVYDLTAEAIIHKKKDYVIQALLANPIVTKARNVKELVEIMIDQQSKWLAYL
                     K"
     gene            9741..>10135
                     /locus_tag="PKW26_06195"
     CDS             9741..>10135
                     /locus_tag="PKW26_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93193.1"
                     /translation="MRFYIKNYDDEKLSVRFDYNKPLVQVMQNLPKRQWIQDKKTWVI
                     PNTQSDINNLLEKLYNTNLFSYEPSADTFPCSDSPTFTTTKSNVEINKNQVDIKKYQE
                     ILITKHYSNRTVKNYTMWLKNFSNRFCDIP"
BASE COUNT         3202 a         2337 c         1717 g         2879 t
ORIGIN      
        1 tagagatttt ttatgtttta gcgtgaaagt tagaatatgt tttctgtttt aattacacaa
       61 tagtgaaaaa agatggtcta aatcttcttg agaaaagtag gatatttcta agcttccttt
      121 ttctagagat cccttaagct gtacctttgt accaagtttt tctcgaaatt gctcttctaa
      181 gtatgcaagt tcaggatttt gttcttcgac tctagctctt tctttctttt ttgtaacaat
      241 gcccttaccg tttaagctcg ctgccatctg ttccgcttca cgaacagaaa gctctgcctc
      301 cacaattctt gcaaataaaa tatcttgttc ttggctgttt aaaaccgata aaagcgcacg
      361 tgcatggcct gcacttattt ttccttgact cagggcattt ttcatatctt ctggtagttt
      421 taacaagcgc aatgcatttg ccaccgcact acggcttttc cccactcgct tcgctacctc
      481 ttcttgcgtt gcattactca tgagcatgag atcgttatag gcaatcgcct cttccagggc
      541 atttaagtct tctcgttgaa tattttcgat tatggcaatt tcaagcatcg cctcttttgt
      601 atactgcttt atgcaaacgg gaacacgctc taagcctgct atttttgcag ctcgcacccg
      661 tcgctcacca ccgacaatgt agaaatttcc gtcattcgac tgcgaaacaa taatcggttc
      721 gactatccca tgctctttaa tggaagaagc aagctcttca agagcatctt gatcaaaatc
      781 ctttcgtggt tgatacgggt ttggcagcaa aagtcccact tccatctctg tcaaaatagt
      841 ttcaccctca gataaaaccc tctgcccttc gagtggaagg cctttttcac gagcatcctt
      901 ttggcttata tcttgcgtta cgtccgttgg agaaaacgca ttgtccccaa aaacggcatc
      961 tattcccttt cccaagccta gtttagccac gcgcaagcac ctcttttgca agttccttat
     1021 agcttctcgc tccaacacag tttgcgtcat acaggttgat gggtaagcca tgagaaggcg
     1081 cttcagaaag gcgtacattt ctcggtataa tcgtgctaaa gactaggtcc ttaaaatagg
     1141 cttttacttg cataaccaca tcttgagcga gccgtgttct tgaatcgtac atggtaaaaa
     1201 aaataccccc aattttaaga cttgggttta tgctttgttg cacttttttt actgtttgta
     1261 aaagaagcga tattccttca agcgcaaagt actcacactg catggggata aaaacttcat
     1321 ttgcacaagc caaaccattc agggttaaaa tacccaaaga tggagggcag tctataagta
     1381 tataatcgta gtctttttta agcacttcta ttgcattttt taggtaataa tctctgtcat
     1441 cttgatcgac caattcaata gcagcacctg ccaagtcgat agaggcagga atagcctcca
     1501 gatttttttg ctttgtttgt tttataacgt catttgctgt agcaagctcg gcgatgagtt
     1561 cgtagactgt tggtttttcc ttgcttatac caaccccact tgacatattt gcctgagaat
     1621 caaagtcaac gagaagcact tttttacctg cttctgcgag ataggcaccg agatttataa
     1681 cggaggttgt ttttccaact ccaccctttt gattgacaaa tacacaaacc tttgacattt
     1741 tttaactata tcattttttt ttatttacgt atatactttt attctataga gggaaatagt
     1801 gtttacaatt tacgatttaa tacaaaactt gcctgcatat gttgtgcatg ccgatgaaga
     1861 tagcttacac agcgaaatta ccgccatagt tgccgcagat cttatgagcg acgtccttgt
     1921 ttccgtagat gcaccctgcg tccttgttac aagtcttgca agcgaacaag taatccgcac
     1981 ctgcgacata agcggtatac aaacaatact cttggtcaac gacaagttgc caagtcctgc
     2041 aatgaaggcg cttgcagaag aaataggaat tacactttta gcaacaccgc tgcccatgta
     2101 tgaagcttgt gtaaatatcc atttgcttaa aacttttgca gactaagaga tatgaaagca
     2161 aatctcaaaa caattcttgc cctaacaata aaagcaagcc tttcaaaacc cacaaaaaaa
     2221 agcgaactga acaaggttaa aataaagctc ataacaaagg agccacatct cttttatata
     2281 gaagaatata caaaaactca aagttttcac aaaacactca actacgatga atgcctcacc
     2341 tatgtaaaaa aaatactagg gaaaaccttt aaagcagctc tttttcaaac cgctgaaaaa
     2401 gactatcagt ttcttacaaa caaacgaggt tttacacggc ttttagaaca agaacctagc
     2461 aaggaaaaaa acgaaacagc gcttgaaaaa acaagccata acaaagaaaa aaactatctt
     2521 ttagcagaag ataggcccat ccccttttta attgccctcg gcatcatgaa taaagaagga
     2581 aaggttctcg caaaaaaaca aaagaagttt cgccaaataa accgctttct agagtttgta
     2641 aacgatattc tcgatgaact ttgcaaaaat acagaaccga cagagaaaaa tcccttatcc
     2701 atcgtagact ttggctgtgg taaatcctac ctcacctttg cgctctatca ttttatagtt
     2761 gtagaaaaga aaatccctgc aaagattatc ggacttgatt tacgaagcga tgttatacaa
     2821 agctgcacag aacttgcaaa aacctgcaag tataccgacc ttcttttttt taatgacagt
     2881 atagaaaact ttctaagcga taaaaacaat agccaaaaaa atctcgatct tgtcatctca
     2941 ctccacgcct gcaacacagc aactgactac gcccttgcaa gcgcagtgca aaaaaacgca
     3001 aaagttattt tatcggtacc ctgctgccaa cacgaattac acacagccct acaaaaacgg
     3061 cccctcccaa gcgacaaccc ctatgaacca tttggacgct atggcattat tcgcgagcaa
     3121 ttttctgccc ttaccacaga cctcatgaga gcgcttttac tcgaagaagc cggctacaag
     3181 gtgcaagttc tcgagtttat agacattgaa cacaccgcaa aaaatcttct catacgtgcg
     3241 gtaaaacgaa aaacaggctc actcaaagac aaaacaaaga actctcccac accctctaaa
     3301 agcactgaat cgctaaaaat cttacaaaaa gagcttggta tacggctcaa gctcgaaaca
     3361 cttcttgccg acgaaaagcc ttgatgcaag tcaaccgaca gccccaaaaa acaaaacgat
     3421 tatagataga caaagacaga tatctgttga cctttctctt tatccttagt attttagaac
     3481 ttggaggcaa aaatgctaga aacacgcgta aaagaaatat tggaaatggt tcgtccgcaa
     3541 ctacaagcag atggtggcga tttggaattc gttgcccttg aagacggcgg aaaagtccat
     3601 gtaaaattga caggagcttg cggttcatgc ccaatggcaa caatgaccct caaacaaggt
     3661 gtcgaacgag ttctcaaaga aaacatccct gaagtaacag aagttattca agcattctaa
     3721 gaaggacata tataaaaaaa gggctgtcag aagacttaac tttgacagcc ctatgcaaaa
     3781 catagcctat gttttgccac ttataaaagc actccccctt aatctctcca acatctttat
     3841 aactgtaaaa caatttttaa tacgtacttt ttttatctat atgacatttt attcatcttt
     3901 gtaaatcttg caagcggtaa caatagtttt ctcaaaaaaa gaacctcggt tctttaaatg
     3961 tattttcata ttcttttttt ttctccaaaa agacaaaaat ttctcttttt tttagaaatt
     4021 ttccttgctt tttttctagt ttaccagtaa gttggaagaa tgaaagtttc gaaacacaag
     4081 gtagtacgtt ttcactactc aattaaaaat tccagtggag aaatcctcga ttcaactttt
     4141 ggagaagccc cgttggaata cgttcacggt gtggacgcca taatggaagg acttgaaaaa
     4201 gcccttgaag gcaaagaagt aaacagctcc tttgaagtac tcgttcctgc agatgacgca
     4261 tatggaccaa aagtagatga gctcattttc aaagttgcac gaagccagtt tcctcctgac
     4321 gtagaattgg aagttggcga tatcttcaat acaagcgagc aagactacga agttaccatc
     4381 attgaagtaa atgacgacga aatcgtaatt gatgcaaatc atcccctcgc aggagaagac
     4441 cttagctttg acataaatat tctaaacata cgtgatgcaa acgaaagtga aataaaaaac
     4501 ggtctccttg gcttcatcga tgatgaagac tatgaagatg gctgcgattg tggcgatgaa
     4561 gattgtgatt gcgactgtgg tgatgaagat tgtgattgta gcgatgggga ctgctaaaaa
     4621 tacacttcaa caagacgcct gtcgatagcc tctaaaacag aaataaaacc gtcccatttc
     4681 gattttttat ctcggctttg ctctgttttt ttctgagact catcactttg cgtcttttca
     4741 gtaagcatcg ccacataata aaaaatctga tctagtctct gtccccagtt ccctggtagg
     4801 cgaaaagacg aagaaacaat gagttttgaa acaaaaacca ttcctgtcaa aatactcgcc
     4861 cgttttatac cagagtacag agccccactc gtaacttcaa agccaacgac ggtaaaaagt
     4921 acctttccat aggcttgaaa gagattaaaa aaaacaatag aacaaaaaat aaaaagaggt
     4981 gtcaaaaaag cttttatgcg aaaccccttt ttcttttgct taggtctttg aaaatcttgc
     5041 ccctgccggc tatatatttt ggcctttctt ccttccttga tagatacaaa ccactgagag
     5101 cgctttaaaa acaactctga aaaaacaccc aaacctattc ctgttattgt tcctgaaaga
     5161 ataagtaagg gtgctatcaa aaccgcccca gaaccaaaca aaatatagcg cgccaaaaaa
     5221 tactgcacaa gcgtatttgc aagggatcca aacaaagaaa tacccagaca acttacctga
     5281 ttttttgcaa gcctttgtaa aaagagcatg gcaagagcgg aggcaaaact tccccctgca
     5341 gaaaataaaa taatatacga aaaaagagtc ccagaaatga gcgcctgccc aagaactttt
     5401 ataaaaacaa gcaaaaaata ttcacgcgta ttgagtaaaa aaagagcaaa gataataggt
     5461 gcgtttgcaa gacctagccg cataaaggga agcggtttgg ggatagaatt ttcaactgct
     5521 gctaaaaaaa aacaaagaga acctaaaaac aaaataagag cggtattgtc gtgtttcgta
     5581 tctaagacaa gctttggctc tataaatttt tttggatttt cttttttctc atctttgctt
     5641 tgattcaagt agtcatcctc atttttacga cggcgtaaaa gcgccgatac atgtaaaaac
     5701 tagagtcggc gcaaggcatc actggcttct tgatagatgg gacgatactc taaggccttt
     5761 ccatacgcaa tctttgcctc ttcctttttc cccgccatct cgcgaaccat acccaaacga
     5821 taccaccata aggcaatacc aggacttttt cgaacagcca tagaataagc aatgtcagca
     5881 tgttcgtatt ttcccttcaa gcgataaata tcaccaataa aaaaatatgc agtcgaagct
     5941 ctatctccat ttggaagcgc atttacatac tgctcaaaaa gcttgagcga ctccgtataa
     6001 ttgtccaaat aaaaatatgc ctccgcaagc gtttctgtaa tgcggttttc cttagggcta
     6061 atcgcaagag cagtattact tatttttatc gcttcgctat attgttctag gcgaataaga
     6121 gaccaaccaa gcacaacata ggactcgata tttcgactat tttcttttat ttcttctcga
     6181 caaacctctg ccgatttctg gtattttttc tgtgcatccg catatcgctg ggcagcttca
     6241 agatcgcgcc cctcacgata cagttccaaa gcgtcgagtt ttaccggacc aaaattttga
     6301 acagtccttt gcacattttc ttgcactact ggatttggtt gtatcgtaag gtctacattt
     6361 tcttctgcac ataaaacatg caccaaagcc aacccaaaaa caactaagag gatttttaaa
     6421 gaatgtatcg tttttatttt cttcataaag tttattttac cctacttttt agatgctcgc
     6481 aagataggta ttcatagaag cggcagcttt tcgcccctct cccattgcaa ggataaccgt
     6541 agctgctccc aaaacaatat ctccccctgc ccaaacagcg ggcaaagaag ttttaccctc
     6601 agcatctgct ataatttgcc ctcgcaaatc gacatcgagt ccaggcgttg tttgagccat
     6661 gagaggattt gaattatttc ccagggcaat aataaccgca tcgcagtcaa tcaggtgctc
     6721 gctatcttca atgggaacag gacttcgccg cccagattca tccgcctctc ccagctcata
     6781 ggaaagcact tctatcgata cgacacgacc atcttcatca ccaaggatac gggttgggtt
     6841 ttcaagaaag cgaaaaacca cgccttcttc ctccgcatga tcgacctctt cttttcgcgc
     6901 aggcatttca tcccttgtcc gtcgatacac caagctcacc ttctttgcac ccaagcgcaa
     6961 agccattcga gctgcatcca tggcaacatt tcccccacca acgacgacaa catgccccgc
     7021 ttgataaaaa ggcgttgcag caaactcctt gtacgccttc atgagatttg cccgagtgag
     7081 gtattcgttg gcactaaaaa cccctatgag attttctcca gggatattca aaaactttgg
     7141 taagccagca ccagagccga taaaggctgc gtcaaaaccc tcttctttga gaagttgccc
     7201 gagcgttccc gttctaccca caaggtagtt tgtgcgaaac tgcacaccca tttccttaag
     7261 catttctatt tcttttgcaa caattgcctt cggaagacga aactcaggaa taccatagac
     7321 cataacacca cccattttat ggaaggcttc aaaaatagta accgaatgcc cctcacgacg
     7381 cacatcggca gcaagcgtta aaccagcagg gcctgaaccg accaccgcaa ctttttttcc
     7441 tgtttcaggc tttacctcag gtatcgttat gagatttctc tctcgctccc agtccgctac
     7501 aaaacgctca agccggccta taccaaccgc cttatctata tccttaagcg attttccaac
     7561 cgtacaaaca ctttggcact gcaactcttg gggacaaacc cgaccgcaaa ccgcaggcaa
     7621 aagatttgtc gttttgataa tatcaaccgc ttctttaaat gcaccttgag cagccttcgc
     7681 tataaactgt ggaatgggaa catgtacggg acaggcagca acgcatggct gatttttaca
     7741 ttgcaaacaa cggtttgctt caagaatcgc catcgactcc gtaaaaccaa tcgcaacctc
     7801 gcacatttgc cttgaacgct caagaggatt tcttgtaggc atctcctgca agggaatcgc
     7861 aagccgctcc tttggcgaaa aactttgatt ttttatagca tcatcgacag aatcaataaa
     7921 atcaagcgct tttttttcaa gcgcatctat attggtataa ttatcggtat aattcggccc
     7981 agaaactgcc tcacaattcg caacagaatc cacgacctga ctcgcatccg agtcggcaga
     8041 tttttttctt gtatccttca tattcgttta tgtccttgct tttttatcaa gcacttcagc
     8101 cgcagcctcc atgcgacaaa tatgcatatc ttcgctttct tgctttttat acgatttcat
     8161 tcgcaagatc atattgtcaa aatctacaag atgcccgtca aactcaggac catcgacaca
     8221 gacaaacttt gtctcaccgc caacagtaac gcgacaacca ccacacatac cagtgccatc
     8281 gatcattatt gtattgagtg aaacaagcgt atgtatagca aagggacgag ttgtttcaga
     8341 gcaaaacttc atcataatag gtggaccaat cgcaacaacc tcttgcggct tttcattgcg
     8401 ctcacagatt tctttcaagg gctccgtaac caaagccttc ttgccagcag aaccgtcatc
     8461 tgttacaaca aaaagctcat cagccaaaga acgcatctca tcctcgaaga taagcaagtc
     8521 ctttgttcgc gcacccataa ttacgataac cttattcccc acagccttat gcgcttgcac
     8581 gattggatac aagggcgcaa ctccgatgcc tccgcccaca cagacaacag tacccatctt
     8641 ttctatcttt gtgggcttac caagcggacc gagaatagca ggaataaaat cacccttgtt
     8701 tttttgtgaa agtttaagag tcgaagcccc aaccgcctga aagacaagca caatccagcc
     8761 ctcttccttg gacgcatcag cgatagtaag cggaatccgc tcgccaaact ctgtatctat
     8821 ttgcacaagg acaaactgcc ccgcctttct atttcgtaca atatcggaag catctacctt
     8881 gaagtagaac acatcagccg ataattgctt tttttctagt atcttatgca taaaaaatcc
     8941 tctaaatctt acaaaaatat gccttctgtc attttagtaa aaagagggtc tttgtacaag
     9001 agatacaata cattgaaaaa ttattttaag tacgcaagcc actttgattg ctggtctatc
     9061 attatttcaa caagctcttt tacatttctt gccttcgtta caattgggtt tgctaataaa
     9121 gcttgaatca cgtaatcttt tttcttgtga ataattgcct cggctgttaa atcatagaca
     9181 ccacaataag cattcaataa tgatgcataa cccttaggca attctccaag atttatgcct
     9241 tgaacaccat ctttattgat tatcgcagga acttcaactg ctatccaatt accaaattga
     9301 acgctcccag caccaaccaa aacaatattc atatttcctc catattttta caaagctctt
     9361 tctaactaaa atatattttg taaactctca gtcataaagg ctcgaaaaac aattgtttct
     9421 tacttttatc acaaaaacat tagaatacaa gaagtttttt ttaaattctt tgttaaaata
     9481 atcaaacacc tgaacagcta agctttttaa tagttttaaa atgtttgaaa acttttatta
     9541 tacccttcca tcttaatcta aaaataaagg aaatctagaa acatctatat caaattcatt
     9601 ttttaaatta tccaatgtat caatataaac agaaggatcc ataatttttc ttgacaaata
     9661 ctactttcga atgactctta atgtagtgag tacgaacaac aaacttgact ccaagtccaa
     9721 ggttgtgtta caatgaaata gtgagatttt atataaaaaa ttatgatgac gaaaaattgt
     9781 ctgtaagatt cgattataac aaacctttag ttcaagttat gcaaaactta cctaaacgac
     9841 aatggattca agacaagaaa acttgggtta ttcccaatac acaatcagac ataaataatc
     9901 tcttagaaaa gctatataac actaacctat tttcatatga acctagtgct gatacctttc
     9961 catgttcgga ctctccaact ttcacaacta cgaaaagcaa tgttgaaata aataaaaatc
    10021 aggtagatat taaaaaatat caagaaatac tcatcacaaa acactatagc aaccgaacag
    10081 taaaaaatta cacaatgtgg ttaaaaaact ttagtaaccg attttgtgat atacc
//
LOCUS       DAOSSW010000064        10084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_6480_length_10084_cov_6.180377, whole genome shotgun sequence.
ACCESSION   DAOSSW010000064 DAOSSW010000000
VERSION     DAOSSW010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6480_length_10084_cov_6.180377"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..79
                     /locus_tag="PKW26_06200"
     tRNA            6..79
                     /locus_tag="PKW26_06200"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40..42,aa:Pro,seq:ggg)
     gene            complement(233..2527)
                     /locus_tag="PKW26_06205"
     CDS             complement(233..2527)
                     /locus_tag="PKW26_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03545 family protein"
                     /protein_id="HOQ93194.1"
                     /translation="MSEDKKIKKTKKVKQPKTFSIKKLPAFYKKKKNLKKIEKKIFKG
                     LHIANDESFVRSLYMQKDGQYFLIEDKSFTKKDIARLKKIAKDIKSQKAFRINLLPLA
                     ISLACIFALVLSVITFKDLVLKIAVQKIGESIFQAKCDIDYAKIELLNAHIRLDGIQQ
                     ADKNAPMKNLFEIDRIDIDFDLLQLLKKKFVSENIEVRNIAFGTDRKSSGFIPQKEKK
                     QKEKKEGDGFDFNALLSLPIIQDEKNDIIESLSSFNPETILSNLNDSLRSPDLAKTVI
                     DDVELLYRKYEKLPNEFEIASKDVQEKIKEIQAIDIEELKKNPTKIPEAISLVQSSLD
                     LTKEIKTKIDKTSSELQGDVKKVSAYSKEIQTAFNADLTFVSKEINSLTSFRLGDAKT
                     IMTDELEKIAIRSLGKYYPYAVLGLEKVKEFSTKPKKEVKPKKEKKRIIRTGRIITYK
                     KDTKPSILLQNIHLSSLVSDWDISADIKDISNDMDLWGRPTSAEFIFDTFSIKNFGSL
                     VFDLRESRENLIAVQYDGSGYPMDLSKISQSLMEGAESQKLSIPGLPLISGTSKIQSK
                     LVADDFNVFAIEGSLLLDPCSVQSASFEPEFVYNLYARALQKLNSLDASFKIQNTKDA
                     GFDLKISSDIDAQFIEIFSELMNEEITSIQQAATKEVEAFLRESLAPVYDAIGEFNSI
                     DELIENQKKKLEGYQKEAEKKISELKQIASNRLEEEKQKALEEAQKIIDKGKEEAQKE
                     VDKAKEQGKEVVEDAVKNVLKGFF"
     gene            complement(2540..3046)
                     /locus_tag="PKW26_06210"
     CDS             complement(2540..3046)
                     /locus_tag="PKW26_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03546 family protein"
                     /protein_id="HOQ93195.1"
                     /translation="MLKKIINLLKSLNTNRNPGEIAHAVSIGFILGLVPKGNLLWIFL
                     FILFLFVRINKGALFLITLLASAIAPSLDGLFDSLGYWVLVHPSLSPSFSLLLDIPFI
                     AFTSFNDTLVMGSLCFGLFAYIPLYFLARLFIRLWRNSLLPKLVTIPLFKKLGNLFKI
                     KSAMDILR"
     gene            complement(3117..4472)
                     /locus_tag="PKW26_06215"
     CDS             complement(3117..4472)
                     /locus_tag="PKW26_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOQ93196.1"
                     /translation="MSDYFESSFLKKLSLVAQNNDPIEQDLYQKYDVRRGLRYLDGRG
                     VLVGLTRVGDVVGYEIIDGQKVAVPGKLIYRGYDVEDIIKDTEAQKQFGYEQTVYLLL
                     FGELPTQDELDEFRSFLGSKRFLPENFTEDMIMKAPSPDIMNKLARGVLASYSYDPNP
                     EDVSIANNLRQCIELIARSVTLAAYAYEAKRHYYDGKSMYIHNPKPELSTAENLLRLI
                     RRDKKYTKLEAEILDLALILHAEHGGGNNSSFAVHVVSSADTDTYSAIAAAIGSLKGR
                     RHGGANKQVMDMMDDIKKNVKHWDSETEVKEYLRKIIRKEAFDKSGLIYGQGHAVYTI
                     SDPRTILLRDKAVLLAKEKKMTEEFFLYTLIEKLAPIVFAEEKGDSKQICTNVDFYSG
                     FVYTMLGIPRELYTPLFAVSRMAGWAAHRIEEIAAGGRIYRPAYKSILEQREFIPIQK
                     R"
     gene            complement(4667..5962)
                     /locus_tag="PKW26_06220"
     CDS             complement(4667..5962)
                     /locus_tag="PKW26_06220"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOQ93197.1"
                     /translation="MIDVNGNVKGSSVKMNEDILFCEKLTDLAKHNRSEFLHDFKQAF
                     FEIYGKTNTEVVFFASPARVNIIGEHIDYNGGLVFPAAIDTYLYLAIRKRNDSAILYN
                     DIRFPGEKKFSITDSFAYKKEDEYANYLNGILKIMKDAGYVFDSGFEILLFSTIPAGG
                     GISSSSALEVGFAYAISETFHFDIDRITIAKMGQQSEHDFMNVKCGIMDQFIIAVGKK
                     DHAIVLDTQSLEYRFVPLQLNDYRIVVMNSNKKRQLADSKYNERRSECEKGLAILQKN
                     IAEKAVLDRKIEALCELEVFEFEKIKTIWEDSLIQNRVKHCVYENDRVKKAVTALENN
                     DLSELGQLLCQSHESLRDDYEVTGIELDSLYEEAIKNEGCLGARMTGAGFGGCAIALV
                     HTDFIDRFISNVGEAYTLKTGLCATFFACMAGDGVSKLI"
     gene            6156..7031
                     /locus_tag="PKW26_06225"
     CDS             6156..7031
                     /locus_tag="PKW26_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93198.1"
                     /translation="MISLHNDILKADIDARASLYEHTRFDHAGQIVQVTMHNRHTFLT
                     TETKNPLKRATEGTGLSGEFGIHMPIGYDDCPVGEYFPKIGVGLLLKENTKPYDYFHI
                     YKLKKDTKQIIQESDRELRIISSIEPCRGYAYEYEKRISLDKNQIRISYKLKNTGTKP
                     ILTTEYCHNFVGFNDLNLDTNYTLSFDIPLHKNTFKELANPNDCLIFDTNAMSWQSTP
                     KEDFFIGDLGSIKKWQLENTKTKTALSEEVQFTSPYCNVWGNAHVVSPEIFISVDVKA
                     NETMQWERLYTFKEI"
     gene            7173..8900
                     /locus_tag="PKW26_06230"
     CDS             7173..8900
                     /locus_tag="PKW26_06230"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOQ93199.1"
                     /translation="MQFKELSLHTHLIKGLEKASYFEATPVQEEVLSLALAGDDLYVQ
                     SQTGTGKTAAFLIPTIQSLLANESLSGKKALIMVPTRELAVQVEKEARLLSSFSKLKT
                     ASFFGGVSYEKQIAALKKGVDILVGTPGRVLDLQESGSMDLSEIAVLVVDEADRMFDM
                     GFYPDLRRLIKLLPDAKKRQTMLFSATLNTYVKNLAWEYTVDAKEITIDADSITLDEI
                     EQKLFHISSDKKMELLLGTLEKEKPQSAIVFCNTKKACEIVSKRLALNAVKNEYIMGD
                     LAQSKRLKIIEEVKNGSLSILIATDVAARGIDINDLELVINYDLPNESENYVHRIGRT
                     ARAGKKGKAYSFCSEQDVYNLPAIEKYLESSIPSELADEATLKEDKSKGIYIKLDADI
                     ERPYEKDIRRTTRSSQRELPRQRGQGPKTTERKKHAQGSYQKTNKTRPTGNASTKTRN
                     LAPRMGTGMGTEDLSALSFDERMKLYKERYGQGGNPQSQQRKKGQNRQAKKTDTTAKK
                     KTASPNQKSFAKNEAKPKNAASFNAKNKSQIKATENKYTEIERNKKKAETKQKGFLKN
                     LVSFFNKTK"
     gene            8914..>10084
                     /locus_tag="PKW26_06235"
     CDS             8914..>10084
                     /locus_tag="PKW26_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOQ93200.1"
                     /translation="MITVSDLSLKFSNTPLFKDVNLKFTEGNCYGIIGANGAGKSTFL
                     KILSGEIEQDTGTISVSSGQRIAVLKQDHFAYDEHSVLDTVMMGYPKLYECYKAREEI
                     YAKEDFTEEDGIKASELEGDFAELGGWEAENNIEQMLSGLGLEEEYHSRMMSELDEGQ
                     KVRVLLAQAIFGEPDILLLDEPTNGLDLESIHWLEEYLIEFPNIVIVVSHDRHFLNAV
                     CTTICDIDFGKITMFSGNYDFWYQMSQIMQRQQKDMAKKREEKMKDLREFILRFASNA
                     AKSRQATSRKKVYDKLALEELHVTTRKFPYVHFKSDRDVGNNVLRVEKLNYKHEGIDL
                     LKDFSLTINKGDKIAFVGMEHKAKSAFFDIITDNIKADSGDVYWGQTISHDYLPLD"
BASE COUNT         3258 a         1904 c         1824 g         3098 t
ORIGIN      
        1 tatttcgggc aatagcgcag ttggttagcg tacaagtctg ggggacttgg ggtccccggt
       61 tcgaatccgg gttgcccgaa atatgtttgt aaacaaacca gaaattcaag ccgataattg
      121 tgcgacagga cgtcgcacct caataagccg acagttttgg ttctccccat atattcctac
      181 accaaaactg tatagttcat tttacatgga cgtaaaatga acgtatttat ctttagaaaa
      241 agccttttaa aacattcttt acggcatctt cgacaacttc ttttccctgc tcttttgctt
      301 tatcgacttc tttttgtgct tcttccttac ccttatctat aattttttgg gcttcttcga
      361 gcgccttttg tttttcttct tcaagcctat tgcttgcaat ttgtttaagc tcagagattt
      421 ttttctcagc ttctttttga tagccttcta acttcttttt ttggttttct atcaattcat
      481 caatagaatt aaactcccct atggcatcgt atacaggagc caaagattct cgtaaaaaag
      541 cttcgacttc ttttgtcgcc gcttgctgta tagaagtaat ttcttcattc ataagctcag
      601 aaaaaatctc tataaattgt gcatcgatat ctgaagaaat ttttagatca aaacccgcat
      661 cttttgtatt ttgtattttg aagcttgcat cgagactatt cagtttttga agcgcccgtg
      721 catacaaatt gtagacaaat tctggttcaa aagaagcact ttgtacagaa catgggtcca
      781 aaagcaaaga tccttcgata gcgaatacat taaaatcatc agctacaagt ttgctctgaa
      841 ttttcgacgt accggaaata agaggaagcc ctggaataga aagtttttgc gactccgcgc
      901 cttccatcaa tgattgactg atttttgaca aatccattgg gtagccggat ccgtcatact
      961 gtactgcaat aagattttct cgactttcac gtaaatcaaa aacgagactt ccaaaatttt
     1021 taatagaaaa ggtgtcaaaa ataaattccg cactcgtagg acgcccccat aaatccatat
     1081 catttgatat atctttaatg tctgcagaga tatcccaatc agagacaagg ctagaaagat
     1141 gaatgttttg cagtaaaata cttggctttg tatctttttt ataggtgata attctacctg
     1201 ttcttataat gcgttttttt tcttttttgg gctttacttc tttttttggc tttgtgctaa
     1261 attcttttac cttctctaat ccaagaaccg catagggata atatttgcct aagcttcgaa
     1321 tagctatttt ttcaagctca tcagtcataa ttgtttttgc atcacccaat ctaaaagatg
     1381 taagactgtt tatttctttg cttacaaaag taagatcagc attaaaagct gtttgaattt
     1441 ctttgctata ggctgaaacc tttttaacat caccttgcaa ttcagaactt gttttatcaa
     1501 tctttgtttt tatttccttt gtcaaatcaa ggcttgattg gacaagggaa atggcttcag
     1561 ggatttttgt cggattcttt tttaattctt ctatgtctat tgcttgtatt tcttttattt
     1621 tttcttgtac atccttactt gctatttcaa actcatttgg aagtttttca tattttctgt
     1681 acaaaagctc tacatcatca atgactgttt ttgctaaatc gggagaccga agcgaatcat
     1741 ttaagttcga aagaatagtt tcaggattaa aagatgagag actttcaata atatcgtttt
     1801 tttcatcctg tataatggga agggataaaa gtgcattgaa atcaaagcca tcgccttctt
     1861 ttttttcttt ttgctttttc tctttttgtg ggataaaacc acttgatttt cgatctgtcc
     1921 caaaggctat gtttctaact tcaatatttt cactgacaaa cttttttttg agtaattgca
     1981 ggaggtcaaa atctatgtca atgcgatcaa tttcaaagag atttttcatt ggagcatttt
     2041 tatcagcttg ttgaattcca tcaagtcgaa tatgtgcatt gagtagttct atttttgcat
     2101 aatctatatc acattttgcc tgaaagatcg attcacctat tttttgcact gctattttta
     2161 atacaagatc tttaaatgta atgacactta aaacaagggc aaaaatacat gcgagggaaa
     2221 tagcaagcgg caaaagattt atgcgaaagg ctttttgact ttttatatcc tttgcaattt
     2281 ttttgagacg tgcaatatct tttttggtaa agcttttatc ctctataaga aaatactgcc
     2341 catctttttg catatacaaa gagcgcacaa agctttcatc gtttgcgata tgaagccctt
     2401 taaatatctt tttttctatt ttttttagat tctttttttt cttataaaag gcgggtaatt
     2461 tttttataga aaaagttttg ggctgcttta cttttttcgt tttttttatt tttttgtctt
     2521 cactcatttc tcaccccttt taccgcaaga tatccatagc agattttatc ttaaaaagat
     2581 tcccaagttt tttaaacaat ggtatagtaa caagcttcgg taaaagagag tttctccaaa
     2641 ggcgaataaa aagtcttgca agaaagtaaa gcggtatata ggcaaagagc ccaaaacaaa
     2701 ggcttcccat aacaagggta tcgttaaaac ttgtaaaagc aataaaagga atgtcgagga
     2761 gaagagaaaa gctgggagaa agacttgggt gcactaaaac ccaatagcca agactgtcaa
     2821 aaagtccgtc caaacttggt gcaatagctg atgctaaaag cgttatgaga aataaggctc
     2881 ccttatttat tctaacaaaa agaaataata taaaaagaaa aatccacaaa agatttccct
     2941 tgggaacaag ccccaaaata aaaccaattg aaacagcatg tgctatttct ccagggtttc
     3001 tgtttgtatt gagcgatttt agtaagttta ttattttttt taacatggtc tacctacaca
     3061 gtcctttgat cttagcttag tataccacgt ttaaaacaag ctttccagtt ctttttttaa
     3121 cgtttctgaa tagggatgaa ttcgcgttgt tctaaaatac tcttgtatgc cgggcgataa
     3181 attctgccac ctgcggcaat ttcttctatg cgatgcgctg cccatccagc cattcgagaa
     3241 acagcaaaga gcggtgtgta taattcacga ggaataccga gcattgtgta gacaaagcca
     3301 gaataaaaat ccacattggt acaaatttgt ttgctatctc ccttctcttc tgcaaaaacg
     3361 atgggtgcaa gtttttcgat gagggtatac aagaaaaatt cttctgtcat ctttttttct
     3421 tttgctaaaa gcactgcctt gtcgcgaagt agaatggtac gagggtctga aattgtgtaa
     3481 accgcatgcc cttgcccgta aataagccct gatttatcga aggcttcttt acgtataatt
     3541 tttcgcaagt actcttttac ttcagtttcg ctatcccaat gctttacatt ttttttgatg
     3601 tcgtccatca tatccataac ttgcttgttt gcaccaccat ggcgtcgtcc tttgagggag
     3661 ccaatcgcag cagcaatagc agaataggta tctgtatcag cagaagaaac aacgtgaacc
     3721 gcaaaagatg agttattacc gcccccgtgc tcagcgtgta ggataagcgc aaggtctaaa
     3781 atctctgctt caagcttggt atatttttta tcgcgacgaa taaggcgaag taagttttct
     3841 gctgtagaaa gctcaggctt aggattatgt atgtacatgc ttttgccatc ataataatgg
     3901 cgtttagcct cgtaggcata ggcagcaaga gtaacacttc gcgcaatgag ctcaatacat
     3961 tggcgtaaat tatttgctat gcttacatct tctggatttg ggtcatagga atagcttgca
     4021 agcacaccac gtgcaagctt gttcataata tctggacttg gcgccttcat aatcatatct
     4081 tcagtaaaat tttctggtaa aaatcgcttg ctcccgagaa aagagcgaaa ctcatcaagt
     4141 tcatcctgcg tcggtaattc tccaaataaa agtaagtaga ctgtttgttc atagccaaat
     4201 tgcttttgag cctcagtgtc cttaatgata tcttctacat catagccacg atagataagc
     4261 ttaccaggaa ccgcaacttt ttgaccgtca atgatttcat agccaacaac atctccgaca
     4321 cgggtgagtc caactaagac accacgccca tcgagatagc gtaagccacg acgaacatca
     4381 tacttttgat agaggtcttg ctcaatcgga tcgttgtttt gtgcaacgag cgataatttt
     4441 tttaagaatg aactttcaaa ataatcagac attattattc cttggaaaat agaactcaac
     4501 ttatagtcga tatatattta tgcgtattat ataattttca taattatata atttaaagat
     4561 aaagtcaact agtaagaaaa tcaacgggga agattaaaaa tggtacccta ggctaccgct
     4621 aattgtccag tcgataagaa gttcatcact actactgtat ttagagctat atcagctttg
     4681 aaacaccatc tccagccata caagcaaaaa atgttgcaca gagaccggtt tttagtgtat
     4741 aggcttcgcc tacatttgaa ataaatctgt cgataaagtc agtatgaacg agagcaattg
     4801 cacagcctcc aaaaccagcg cctgtcatac gagcgccgag gcagccctcg tttttaatcg
     4861 cttcttcata caagctatca agctcaatgc ctgttacctc ataatcatca cgaagcgatt
     4921 cgtgggattg gcataaaagc tgccctaatt cactaagatc attgttctct aaagcggtaa
     4981 cggctttttt tacacgatcg ttttcataga cacaatgttt tacacgattt tggataaggc
     5041 tgtcttccca aatagttttt attttttcaa actcaaatac ttctagctcg caaagcgctt
     5101 ctatttttct atcaagcaca gctttctctg caatgttttt ttgcaaaata gcaagtcctt
     5161 tttcacactc gcttcttctt tcattatatt ttgaatctgc gagttgtcgt tttttattgc
     5221 tattcattac aacgatgcga taatcgttta gttgtaaagg aacaaaacgg tattcgaggc
     5281 tctgtgtatc cagcacaatt gcatgatctt tttttccaac tgcaattata aactgatcca
     5341 taataccaca ttttacattc ataaaatcat gttcagattg ctgacccatt tttgcaatgg
     5401 tgattctatc tatatcaaag tgaaatgttt cggagatagc gtaggcaaaa ccaacttcta
     5461 atgcagagga agaagaaata ccaccgccag cgggaattgt actaaacagt aaaatttcaa
     5521 aaccagaatc aaaaacgtag cctgcatctt tcatgatttt taaaataccg ttaagataat
     5581 tcgcatactc gtcttctttt ttatatgcaa aggaatcggt aattgaaaac tttttttcgc
     5641 cagggaaacg gatatcgtta tataaaatag cgctatcatt tctctttcga attgcaagat
     5701 ataaataggt atcaatcgca gcaggaaaga ctaatccgcc attataatca atgtgttcac
     5761 caattatatt tacccgtgca ggtgatgcaa aaaaaacgac ttcggtattt gttttcccat
     5821 atatttcaaa aaatgcttgt ttaaaatcat ggagaaattc agagcgatta tgttttgcca
     5881 agtcagtcaa cttttcacaa aacaatatat cttcattcat ctttactgaa gaccctttta
     5941 catttccgtt tacatctatc ataaggactc ccattattat aaatcgctta ttgatagaaa
     6001 atatcatgta ttatacttat ctgcaacagg tattgctgtc aaatacagat atcgcttagt
     6061 ttgtatcaga aatttgccaa aattaaattt aaaacaagtc tatttttatt tttttagttg
     6121 actttcttta ttttcggcta tagactgagt acactatgat tagtttacat aatgatattt
     6181 taaaggcaga catagatgct agggcatctt tatatgaaca cacgcgcttt gatcatgctg
     6241 gacaaattgt gcaagttact atgcataata ggcatacttt tttgacaaca gaaacaaaaa
     6301 atccgctcaa gcgtgcaacc gagggaacag ggctttctgg agaatttggc attcacatgc
     6361 ctataggcta cgatgactgc cctgtcggtg aatattttcc aaaaatcgga gttggtttat
     6421 tgctcaaaga aaatacaaag ccctatgatt attttcatat atataaattg aaaaaagata
     6481 caaaacaaat tatacaagaa agcgatagag agcttcgcat tatttcttcc attgaaccat
     6541 gtaggggata tgcttacgag tatgaaaaac gtatcagcct ggataaaaat caaatacgta
     6601 tttcttataa gctaaaaaat actggaacaa agcccatcct tacaacagaa tattgtcata
     6661 attttgtagg tttcaatgat ttaaacctcg atacgaatta tacactcagc tttgatattc
     6721 ctctgcataa aaatacgttt aaagagcttg caaatccaaa tgattgtctt atatttgata
     6781 caaatgcaat gagctggcaa agcacaccta aggaagattt tttcatagga gatcttggct
     6841 ctatcaaaaa atggcagtta gaaaatacaa agacaaaaac tgctctgtca gaagaggtgc
     6901 agtttacatc tccgtattgc aatgtatggg ggaatgctca tgtcgttagc cctgaaattt
     6961 ttatttcagt tgatgtcaag gcaaacgaaa caatgcagtg ggaacggctc tatacattta
     7021 aagaaatcta aacctaaaat aacaatacct agctaagatg taggctttaa attgtataac
     7081 ttaaatgatc tgcacttgct tctctccttt accttatttg aaaaaaatgc tattctttgt
     7141 aatatcatac ggtttattaa cataaggtaa atatgcaatt caaagaatta tctctccata
     7201 cacatctcat aaagggtctt gaaaaggcct catattttga agcaactcct gtgcaggaag
     7261 aagtcttatc tctcgcgctt gctggagatg acctatacgt acaatcccaa acaggaacag
     7321 gaaaaacagc agctttttta atacctacaa tacaaagtct acttgcaaat gaaagtttga
     7381 gtgggaaaaa agcgcttatc atggtaccga ctcgagaact cgctgtgcaa gttgaaaaag
     7441 aagctcgctt actctcttca ttttctaagt taaaaacagc aagttttttt ggtggtgtta
     7501 gctacgagaa acaaatagca gccctcaaaa aaggtgtaga catcctcgtt ggaacacctg
     7561 gacgagtgct cgacttgcaa gaaagtggca gcatggactt aagcgagatt gctgttctcg
     7621 ttgtcgatga ggctgaccgc atgtttgaca tgggctttta tccagattta cgtcggctca
     7681 taaaactcct gccagatgca aaaaaaagac aaaccatgct ttttagcgct accctcaaca
     7741 cctatgtaaa aaatcttgct tgggaataca cggtcgacgc caaggaaatc accattgatg
     7801 ctgattctat tacccttgat gaaatagagc aaaaactctt tcatatctca agtgataaaa
     7861 aaatggagct gcttctcggg acacttgaaa aagaaaaacc ccaaagcgct attgtttttt
     7921 gcaacacaaa aaaagcttgc gagattgttt caaaacgtct cgctttaaat gcagttaaaa
     7981 atgaatacat aatgggagac cttgcacaat cgaagcgtct taaaataatc gaagaagtaa
     8041 aaaatggctc cttatcgatt ctcattgcaa ctgatgttgc tgctcgcggc atcgatataa
     8101 acgatttaga actcgttatc aattatgatt tacccaatga aagcgaaaat tatgtgcatc
     8161 gcatcggaag aacagcaagg gcagggaaaa aaggtaaggc ctacagcttt tgtagcgagc
     8221 aggacgtcta taatcttccc gcgattgaaa aatatttgga aagcagtata ccatctgagc
     8281 ttgcagatga agccacatta aaagaagata agagtaaggg tatatatatt aaactcgatg
     8341 cagatatcga gcgcccctat gaaaaagata tacgaagaac aacgaggtca agccaaagag
     8401 aactacctag gcaaagagga cagggaccta aaactaccga gcgaaaaaaa catgctcaag
     8461 gctcctacca aaagacgaat aaaacaagac caacgggaaa tgcttcaacg aaaacgagaa
     8521 atctagcccc tcgaatggga actggtatgg gcacagaaga cttgagcgca ctttcttttg
     8581 atgagcgaat gaagctctat aaagaacgct atgggcaagg tggaaatcca cagagccaac
     8641 aaaggaaaaa agggcaaaac cgacaagcta agaaaacaga tacaactgca aagaaaaaaa
     8701 cggcaagtcc gaatcaaaaa tcttttgcta agaacgaagc aaaaccaaaa aatgccgctt
     8761 ccttcaatgc aaaaaataaa agtcagataa aagcgacaga aaataaatat accgagattg
     8821 agagaaataa aaaaaaggca gagacaaaac aaaaaggatt tttgaaaaac cttgtctctt
     8881 tttttaataa aacaaagtaa ggattcattt tatatgatta cagtaagcga tttaagttta
     8941 aaatttagca acactcccct tttcaaagat gtaaacttaa aatttaccga gggaaattgt
     9001 tatggcatca ttggtgcaaa cggtgcagga aaatcaacct tcctcaaaat cttatctggc
     9061 gaaatagagc aagatacggg aactattagc gtatcaagtg gacagagaat tgctgtgctt
     9121 aagcaagacc actttgccta tgacgaacat tcagttttgg ataccgtgat gatgggctat
     9181 ccgaaactct acgaatgcta caaggcccgt gaagaaattt acgcaaagga agattttacc
     9241 gaagaagacg gcatcaaggc aagtgaactt gagggagact ttgccgagct cggtggatgg
     9301 gaagcagaaa acaatataga acaaatgctc tctggtcttg gcttagaaga agaataccat
     9361 agccgcatga tgagcgaact cgatgaaggg caaaaggtac gcgtgctttt agcgcaagct
     9421 atttttggag agcctgatat cctccttttg gatgagccga caaacggctt ggacctagag
     9481 tccattcact ggcttgaaga atacttaatc gaatttccga acattgttat tgtggtaagc
     9541 cacgacagac actttttaaa tgcggtttgt acgacgattt gcgatattga ctttggtaag
     9601 attactatgt ttagtggaaa ctatgatttt tggtatcaga tgagtcagat aatgcaacgg
     9661 caacaaaagg atatggcaaa aaaacgtgaa gaaaaaatga aggatttacg agagtttatt
     9721 ttacgttttg cctcaaacgc tgcaaaaagt cgacaagcga caagtagaaa aaaagtctac
     9781 gacaaactcg cccttgaaga actacatgta accactagaa aatttcccta cgtccatttt
     9841 aaaagcgaca gagatgttgg gaacaatgtg ctacgagtcg aaaaactcaa ctataaacat
     9901 gaaggtattg atttactcaa ggattttagt ctcactataa acaagggaga taaaatagcc
     9961 tttgtgggaa tggagcataa agcaaagtca gctttttttg acataattac ggacaatata
    10021 aaagcagact caggcgatgt ctactgggga caaacaataa gccatgacta tcttcccctc
    10081 gata
//
LOCUS       DAOSSW010000065         9976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_6568_length_9976_cov_5.455700, whole genome shotgun sequence.
ACCESSION   DAOSSW010000065 DAOSSW010000000
VERSION     DAOSSW010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9976
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6568_length_9976_cov_5.455700"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1738
                     /locus_tag="PKW26_06240"
     CDS             65..1738
                     /locus_tag="PKW26_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOQ93201.1"
                     /translation="MSFTQKLRYIFRKYPSLRFVSLLVLIFIVFFSLVFFSRGIQKAP
                     HLYGISPQIANPGDVFTLTGQFFGSEQNDSYVEIGGNPLTQSSYLEWSENSIRLTLPA
                     NIQDGLVYVVTKAGRSEPKNFANRQSIPILVKKESTLHTPRISSVSEKKPAVGNLISI
                     QGANFSSLRGSSKVYFSLSDTEDDTTEYIACSETNPDYDFWSDELIRLRVPDGAQSGN
                     IYVETDTGKSNFFPIQINTKVGTKTHKNKNTYLLSLHGDMTDIETMGESTLTLRIPRP
                     IYSPLQELIEITQSKPEPTIADHMGTIVHQFPISKGITEKIEVNHNFVVLARSLETQI
                     AEKEILPYSDKSLSMYAEYLKPNSLIVSDKAEIQELAKKITKGTTNVYTKARRIFDYI
                     KEKFTLDVELRENERNLQFALELKTLDSYEAALLFTSLCRAAEIICLPVAGVLIDANL
                     IAKNHWWTEIYLENFGWLPVDMSLALGLEYQGFHINEKKDADFYFGNIDSQHIAFSRG
                     LNTIKPTNQTGKIVTRNRSYALQTIWEEASAQILSYSSFWSPIYVNGVY"
     gene            1738..2427
                     /gene="pyrH"
                     /locus_tag="PKW26_06245"
     CDS             1738..2427
                     /gene="pyrH"
                     /locus_tag="PKW26_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOQ93202.1"
                     /translation="MIKVLSVGGSIVVPQEPDLAFISQFLSLVKEYLSKDIKRKLILV
                     IGGGGPARLYQNAYKTIVNDDTKNNNEADWIGIMATRLNAQLIKASLGDLCSDEVVID
                     PSAAFTFSGRVLVAAGWKPGFSTDTIATLLAERFEAKTLVNLSNIEKVYTDDPKLNPE
                     AKPLDSISWETFRAMVGDEWTPGKNTPFDPIASKKAESLGLKVICAGGRNIDNTKAIL
                     EEKDFFGTTIS"
     gene            complement(2389..3048)
                     /locus_tag="PKW26_06250"
     CDS             complement(2389..3048)
                     /locus_tag="PKW26_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOQ93203.1"
                     /translation="MNTKNNYTIQKQLVSMIFSFFLLSFLLSCLSSPQFAIEKEEKKL
                     SRLICMKGLLEPRELSHFFMQENPHADAQKVWALANFYVKEAQDEGINSDIAFVQMCL
                     ETGFLRFGGLVSEDMNNFCGLGAISREEPGLRFPSMEIGVRAHIQHLQAYACTRDLQK
                     ELVDPRYKWVQPRGKSPSIYGLAGTWASDQAYGEKLDALLERLESFLLTNCCSKKIFF
                     F"
     gene            3127..3816
                     /gene="radC"
                     /locus_tag="PKW26_06255"
     CDS             3127..3816
                     /gene="radC"
                     /locus_tag="PKW26_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOQ93204.1"
                     /translation="MKTGNMYKTTNPILNDENGKPQIRERALHYGFSDLNDHQLLMML
                     IGNGIKGCPVDELAEKVLVIIRNPSISDYAQALKEIPGIGPSKATLIGAAIELGKRFH
                     AKNTSIIRSPEDILPFFMHISLAPNESFWCASLNGAHEVIKIRETSKGSVNKSIVDVR
                     EVFCEPLKERACGIIVCHNHPSGNLSPSNNDIEVTKQLKKAGEILNIQVLDHLIVSSQ
                     GYLSFSEQGFL"
     gene            3905..4468
                     /gene="efp"
                     /locus_tag="PKW26_06260"
     CDS             3905..4468
                     /gene="efp"
                     /locus_tag="PKW26_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOQ93205.1"
                     /translation="MIRGGDINKGTVLLIKNAPYLVVEREFVNPGKGSAFARVKMKNL
                     KDGSVLTQTIKTPDMVEDAVVETVDAQYQYSDGENFVFMNNTTFDTINVPYTLNDTLQ
                     YYLKEGESYPVVIWEEEPIDVKIPPKMVFIVAESENYIKGDTVSGATKPIVTETGLTV
                     RVPLFIKQDEKIMVNTETNEYVERVNN"
     gene            complement(4540..4613)
                     /locus_tag="PKW26_06265"
     tRNA            complement(4540..4613)
                     /locus_tag="PKW26_06265"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4577..4579),aa:Val,seq:gac)
     gene            complement(4727..5161)
                     /locus_tag="PKW26_06270"
     CDS             complement(4727..5161)
                     /locus_tag="PKW26_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ93206.1"
                     /translation="MKTKQDFPSINERPPDGLKDDGTKVRVLVVDDSIFVAKQIGQIL
                     SSEGYEIVATAVDGKEGVDKYKDMFPNVDIVTMDITMPRMDGITALEQIMAFDKNAKV
                     VMISALGKEELVKKSLLLGAKNYIVKPLDRKKVLERISSVLK"
     gene            complement(5178..5642)
                     /locus_tag="PKW26_06275"
     CDS             complement(5178..5642)
                     /locus_tag="PKW26_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOQ93207.1"
                     /translation="MRVEYINPFVETAYSILVEVLGGEVKRDNLYLKSTSMPVMGVAA
                     LVGLAGDVEGRVLFDMSMETALNIASKMNDEKIDTFDELAKATITELANLITAQAVTK
                     LHELGFKFDLTPPALFTGERMEISDQNVEALIVPMITEQGKIEVNVAIRERM"
     gene            complement(5676..7034)
                     /locus_tag="PKW26_06280"
     CDS             complement(5676..7034)
                     /locus_tag="PKW26_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOQ93208.1"
                     /translation="MENTIEQTLMQETSYDIEDQNDKISIIDFKMITFSLSGKDYAID
                     IMKVKEIAKAGHFTYVPNTSSFVLGVHNLRGDIIPIIDLRIFFNIPVEERKDDDLENL
                     IVVSVGEQSFGVVVDHIDKVVGIQQSSIQPPHPLFGDINIKYISGVVENANRLFILLD
                     IDRIFGLRQAPTVESAKEQEHFSPAQKTIADHKPTQKQETPVLEHKASDLDYNFLVED
                     LKVRKSFNLSLINEAWIKSRYMKWLDERDAENIQFNSDEDAELFLKPFASKFSHDFWE
                     ESYAEQLYKALPENTAKQITVWNPGCGKGHESFSLACVLKKRYPEARIRIYAQDTDLL
                     AVSSAQMLRVPQNQAESWYAPFLTKTVSGEHVFNADITEMLFFEYHDITHNNALPNVD
                     IIFCRDVLSYLDKEKQEIVLEDFEDKLKGNGVLIIGDNEVLTNYVDWHSKEVASITMY
                     TK"
     gene            complement(7044..9455)
                     /locus_tag="PKW26_06285"
     CDS             complement(7044..9455)
                     /locus_tag="PKW26_06285"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOQ93209.1"
                     /translation="MSDYLDLNNEELLKDFFSEAEQQVQALESNILVIETDPSNTEAI
                     DEIFRAAHTLKGGSATVEMHELSGFTHVVEDLLDVLRAKKISVSEEVVDTLLNAIDVI
                     KEMLQYRSNGSVYTDDVEPLKETLKSFIPAKGEKTKSTPPKSLASFQTPQHTASPALP
                     TMQDPAELLSEYDLLELNESRPSDDPVWGITVIFDESNPMNSVGGIQVFSALKDKGAV
                     LKTIPDFDVLYDDEFHEKVVYFISSALNAEQLEDAAFMDDVTLAVDAKKIDSQKSTFS
                     SPEKSFKDTPTPTSAAKYEESRQEIAASIEAKQSAKEVTAFNEHIQEETDALEKKKKV
                     APTGQQTSTSSILRVDSKRIDYLLNLVSETVITKASFNQITTQMGDLQTQLASTQALY
                     REKIRSLFDKLPSFIETLKEGDTIKDIRTRLNTEYADIFSIFESFDSSFKGANTKLRS
                     ASQNLGRIAGELQEGVMKIRMVPVNQIFSRFPRVIRDLSRDLDKKIHLEIEGEDTELD
                     KSVIEDLLDPIMHCVRNSIDHGIERPEERAKKGKSEEGTVLLKASNEGNMIVIDIQDD
                     GAGIDVEKVKQKAIERGLIHPNKILSDHEAHQLIFEAGFSTAAKVSNVSGRGVGLDVV
                     KTQIEKLNGSVIVSSEPGNGTKFSIRLPLTLAIIQGLLIRVGKEVYSIPIASVLESHR
                     VQYEDIQTIDNYEVFNVRNEIISVLRLNRLFGIKQTDDEDFCFIVIVGSAEKKIGIMV
                     DALIGEEDVVIKPLRDQFTVSPGIAGASILGDGSVSLIIDVSKLLELGVKQELSAREA
                     LEIGI"
     gene            9613..>9976
                     /locus_tag="PKW26_06290"
     CDS             9613..>9976
                     /locus_tag="PKW26_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93210.1"
                     /translation="MKKKYNTKIRHIVLVIALFFVGFQGYAAPSSNGIIGAGLLIQPM
                     PHELPDLFAEAFFASQIDLSQSLLIRTEFSVFTDSIIGDNFLKDIPAYFSLNELSLTY
                     VGSLGTISQLASVFIGETD"
BASE COUNT         3159 a         2007 c         1739 g         3071 t
ORIGIN      
        1 ctttccaaaa aataaattct gatttttatc aagcatcgta ttatacaaaa ggtagattcc
       61 tgccttgagt tttacacaaa agctacgcta tatttttcga aaatatcctt cactgcgatt
      121 tgtaagcctc cttgtgctta ttttcattgt gtttttcagt cttgtctttt tttcaagagg
      181 catacaaaag gcgccacatc tttacggtat tagcccgcaa atcgcaaatc caggagatgt
      241 atttacgctt actggccaat tttttggtag tgagcaaaat gattcgtatg tagaaatagg
      301 tggaaatccg cttacacaat caagttacct cgagtggtct gaaaactcta tacgtctcac
      361 tttacctgcc aatatacaag atggtttggt ctatgttgtt acaaaggcag gacgaagcga
      421 gcccaaaaat tttgcaaata ggcaatctat acctattctc gtaaaaaaag aaagcacctt
      481 acatacgcca cgtattagca gtgtttctga aaagaaacct gcagttggaa acctcataag
      541 tattcaagga gcaaactttt cttccctccg tggtagttcg aaagtgtatt tttctttgag
      601 tgatacagaa gacgatacaa ctgaatatat tgcttgttca gagacaaacc cagactatga
      661 tttttggagt gatgaactga tacgactacg tgttcctgat ggcgcacaaa gtggaaatat
      721 ctatgtggaa acagatactg gcaaaagcaa ttttttcccc attcaaataa acacaaaggt
      781 aggcacaaaa acacataaaa ataaaaacac ctatcttctc agcctacacg gcgatatgac
      841 agatattgaa acgatgggag aaagcactct cacattgcgc attccaagac cgatatattc
      901 gccattgcaa gaacttatag agataactca atcaaagcct gagccaacta tcgccgatca
      961 catgggcacg atagtgcatc aattccctat ttcaaaaggc attactgaaa aaattgaagt
     1021 taaccataac tttgtcgttc tcgcacgctc acttgaaaca caaattgcag aaaaagaaat
     1081 actcccctat tctgataaaa gtttaagtat gtacgcagaa tacctaaagc caaacagtct
     1141 cattgtatct gataaagcag aaatacaaga actagcaaag aagataacaa agggcactac
     1201 caatgtctat acaaaggcaa gacgtatttt tgattatatt aaggaaaaat ttacactcga
     1261 tgttgaatta cgagaaaatg agcgaaattt acagtttgcc ctcgaattaa aaacacttga
     1321 tagctatgaa gcagctcttt tatttaccag tctttgccga gcagctgaaa ttatttgcct
     1381 tccggttgct ggagttttaa tcgacgcaaa tttaattgca aaaaatcact ggtggacaga
     1441 aatttatcta gaaaattttg gttggcttcc tgtcgatatg tccttggcgc ttggtttaga
     1501 gtatcaaggg tttcacataa acgaaaaaaa agatgccgat ttttattttg gcaacattga
     1561 ttcgcaacac atagcctttt cacgtggatt aaacactatt aaaccaacaa accaaacagg
     1621 aaaaattgtt acgagaaatc gttcctatgc tttgcaaaca atttgggagg aagcctccgc
     1681 ccaaattcta agctatagca gtttttggag cccaatatat gtaaatggag tgtattaatg
     1741 attaaagtcc tttctgtcgg tggctcgatt gttgtgccac aagaacccga tcttgctttt
     1801 atttctcagt ttctttctct tgtaaaagaa tatctaagta aagatataaa gcgaaaactc
     1861 atacttgtaa tcggtggcgg cggtcctgca cgtctttacc aaaatgccta taagacaatc
     1921 gtcaacgatg atactaaaaa caacaacgaa gccgattgga taggtataat ggcaacacga
     1981 ctcaatgcac agcttataaa agcaagtctc ggagatttat gcagcgacga agtagtaata
     2041 gacccgagcg cagcgtttac attctctggc cgcgttcttg tagcagctgg ctggaaacca
     2101 ggcttttcaa cagacactat tgcgaccctt ttggcagaac gctttgaggc aaaaaccctt
     2161 gtcaatttat caaacattga aaaagtctat accgatgacc caaaattaaa tcctgaagca
     2221 aaaccgcttg atagtatttc atgggaaaca tttcgcgcca tggttggtga tgaatggact
     2281 ccgggaaaaa acactccctt tgatcccatt gcaagtaaaa aagcagagtc gctcggctta
     2341 aaggttattt gtgctggcgg tcgaaacata gacaatacaa aggcaatttt agaagaaaaa
     2401 gatttttttg gaacaacaat tagttaaaag aaagctttcc aatcgctcta aaagtgcgtc
     2461 tagtttttct ccataggcct ggtcactcgc ccaagttccc gcaagcccat aaatcgaagg
     2521 tgatttaccg cgtggttgca cccatttata ccgcggatcg acgagttctt tttgtaaatc
     2581 cctcgtacag gcataagctt gcaaatgctg aatgtgtgcg cgaacaccta tttccatgct
     2641 aggaaaacgt aagcccggct cttctctact gatagcacct aagccgcaga aattgttcat
     2701 atcttcgcta actaagccac caaaacgcaa aaaacctgtt tccaaacaca tttgcacaaa
     2761 ggcaatatct gagtttatcc cctcatcctg ggcttctttt acatagaagt ttgcaagcgc
     2821 ccataccttt tgtgcatctg catgaggatt ttcctgcata aagaaatggg acaattccct
     2881 tggctctaaa agtcccttca tacagatgag acgagagagt tttttctcct ctttttcaat
     2941 tgcaaactgc ggtgaactaa ggcacgaaag gagaaaagaa agaagaaaaa aggaaaaaat
     3001 catcgaaaca agctgctttt gtattgtata gttattttta gtattcatat tttttatata
     3061 tcggtttttt tattttaaaa tacataaaac tcgtgcaaaa aaccaataat acatgtagga
     3121 ggcaaaatga aaacaggaaa catgtataag acaacaaatc ctattttaaa tgatgaaaac
     3181 ggaaaaccac aaattcgcga acgagcgcta cactacggtt tttcagactt aaacgaccac
     3241 caacttctga tgatgctcat aggcaatgga ataaaaggtt gtcccgtcga tgaacttgca
     3301 gaaaaggtat tggtgattat tagaaaccca agtatttctg attacgccca agcattaaaa
     3361 gaaattcccg ggataggccc atcaaaagca acgcttatag gagctgcaat agagttgggg
     3421 aaacgatttc atgcaaaaaa cacgagtatt atccgctccc ctgaagatat tctccctttt
     3481 tttatgcata tatcactagc cccaaatgaa tctttttggt gcgcatctct taatggcgca
     3541 catgaggtta taaaaattcg tgaaacaagt aagggctccg taaataaatc cattgttgat
     3601 gttcgggaag tcttttgcga gcctctaaaa gagagagcat gcggaattat agtctgtcat
     3661 aatcatcctt cgggaaattt aagtccctca aataatgata tcgaggtaac aaaacaactt
     3721 aaaaaagcag gcgaaatctt aaatattcaa gtattagatc atttaattgt cagctctcaa
     3781 ggctatctta gtttttcaga acaaggattt ctttagaaaa aatacctgca aaaacagagt
     3841 attggcataa agtcgttttt tttgtataat tatagagaaa aatacataga aagtgaggaa
     3901 ttgaatgatt agaggtggag atataaataa aggaacagtc ttactcataa aaaacgcacc
     3961 ctatctcgtg gtagagagag aatttgtgaa cccgggaaaa ggttctgctt ttgcaagagt
     4021 taaaatgaaa aatctcaaag atggttctgt tttaacacaa acaataaaaa caccagacat
     4081 ggtcgaagat gcggtagtgg aaactgtaga tgcacagtac cagtactctg atggtgaaaa
     4141 ctttgttttt atgaacaaca ctacttttga cactataaac gtaccctata ctctaaatga
     4201 taccttgcag tattatctta aagaaggcga gtcctatcct gttgttatct gggaagaaga
     4261 acctatcgat gtgaaaattc caccaaagat ggtttttatt gttgctgaaa gtgaaaatta
     4321 tataaaaggc gacaccgttt caggtgcaac aaaacctatt gtcacagaaa caggcttaac
     4381 tgttcgcgtt cctttgttta ttaaacaaga tgaaaagatt atggtaaaca ccgagacaaa
     4441 cgaatacgtc gagcgtgtaa acaactaacc tttttaaaaa gcaaaattac aattgtttta
     4501 ataaaaaaaa gaactgccta tacagacagt tctttttttt gggcgatatt ggaatcgaac
     4561 caatgacccc aagcgtgtcg agcttgtact ctaaccagct gagctaatcg ccctaattgt
     4621 ttatatacta ataaaaaaca aagattaggt caagtacaaa aaaagctact aagtattgct
     4681 ttttcaacaa taattagtag cttacaacaa aacataaaaa aatcttttac tttaagacag
     4741 aactaattct ctctaagact tttttcctat ctaatggttt tacaatataa tttttggcac
     4801 ctaggagaag agattttttt acaagctctt cttttccaag agcgcttatc ataacgacct
     4861 tcgcattttt atcaaaagcc ataatttgct ctaaagccgt aattccatcc attctcggca
     4921 tcgtaatgtc cattgtaaca atatcaacat ttggaaacat atccttgtac ttatctacac
     4981 cttctttgcc atctactgct gtagcaacta tttcataacc ttcactgctt aatatttgcc
     5041 caatttgttt tgcaacgaaa atggagtcgt ctacaacaag aacgcgtacc ttggtaccat
     5101 cgtcttttaa accatcaggt ggacgctcat taattgaagg aaaatcttgt tttgtcttca
     5161 tagcctacca ctccttttta cattcgctca cgtatagcga catttacttc tattttacct
     5221 tgctccgtta tcattggaac aattaaagcc tcaacatttt gatctgaaat ttccatcctc
     5281 tcacctgtaa ataaggcagg tggcgtaaga tcaaacttaa aaccgagctc gtgcaacttt
     5341 gttacagctt gtgctgtaat taagtttgcc agctctgtta ttgtagcctt tgctaattca
     5401 tcaaaggtat ctattttttc atcgttcatt ttcgatgcaa tatttaatgc tgtctccatt
     5461 gacatgtcaa ataaaacacg accttcaaca tctccagcca aaccaacgag ggccgcaacc
     5521 cccataacag gcatcgaagt tgattttaaa tagagattat cccgttttac ttcaccgcct
     5581 aaaacttcta ctaaaatact gtatgcagtt tcgacaaacg gattaatata ctcgactctc
     5641 attatgttcc cctttaaatt attttatatt atttgttact ttgtgtacat cgtaattgat
     5701 gccacctctt tactatgcca atcaacataa ttcgttaaca cctcattgtc accaattatt
     5761 aaaacaccat tacctttcaa tttatcttca aaatcttcca aaactatctc ttgcttttct
     5821 ttatctaaat aggacaggac atcgcgacaa aaaattatat ctacatttgg tagcgcatta
     5881 ttgtgcgtta tatcatgata ttcgaaaaaa agcatttctg taatgtcagc attaaaaaca
     5941 tgttcgccac taactgtttt tgttaaaaaa ggagcatacc aactttcggc ttgattttgt
     6001 ggaacgcgta acatttgtgc actcgaaacg gcaagtagat ctgtatcttg tgcgtagata
     6061 cgaattcgtg cttctggata tctctttttt aaaacacagg caagagaaaa agattcatga
     6121 ccttttccac aacctggatt ccaaacagtg atctgttttg cggtattttc gggaagggcc
     6181 ttatataatt gctctgcata tgattcttcc caaaaatcat gagaaaactt cgatgcaaag
     6241 ggttttaaaa aaagctcagc atcttcatcg ctattaaatt ggatattttc tgcatcacgc
     6301 tcatcgagcc atttcatgta gcgcgacttg atccacgctt catttataag agaaagatta
     6361 aaacttttcc gcactttaag gtcttcaacg aggaagttat agtcaaggtc gctcgcttta
     6421 tgttctaaaa cgggggtttc ttgtttttgt gtaggcttat gatctgcaat tgttttttgt
     6481 gcaggagaaa aatgctcttg ctctttagcg ctttcaacag taggagcctg tcgtaatcca
     6541 aaaatgcgat cgatatcgag taaaataaac aagcgattag cattttcaac aacacccgaa
     6601 atatatttta tatttatatc gccaaaaagt ggatgcggcg gttgaatgct tgactgttga
     6661 atcccaacaa ccttatcgat atgatctact acaacaccga aagattgttc tccaacgctc
     6721 acaacaatga ggttttctaa atcatcatcc tttcgctctt caacaggaat attgaaaaag
     6781 atgcgcaagt caataatggg aataatgtct ccgcgaagat tatgtacgcc gagaacaaaa
     6841 gaagaagtgt taggcacgta ggtaaaatgt ccagcctttg ctatttcctt taccttcatt
     6901 atatctattg cataatcctt tcctgaaaga gaaaaggtaa tcattttaaa atcgataata
     6961 cttattttat cgttttgatc ttctatgtca taagaggttt cctgcataag agtttgttca
     7021 atcgtgtttt ccatatctac ctcctatata cctatttcta aggcttcacg tgcacttaat
     7081 tcttgcttta cgcccaactc taacaatttt gatacatcga taatcaatga aacggatcca
     7141 tcacctagaa tcgaagcacc agcaatccct ggagataccg taaactgatc tcgtagcggt
     7201 tttattacaa cgtcctcctc acctataagc gcatctacca tgatgccgat ttttttctct
     7261 gctgagccaa caataacaat aaaacaaaaa tcttcgtcgt ctgtttgttt tataccaaag
     7321 agtcggttca atcgtaaaac agaaataatt tcatttctca cgttaaagac ttcatagttg
     7381 tcaatagttt gaatatcttc atattgcaca cgatggcttt cgagtacaga ggcaataggt
     7441 atagaataga cctctttgcc aacacgaata agtaaacctt gaataatagc aagtgtcaat
     7501 ggaaggcgaa tactaaattt tgtaccattt cctggctcag aactaacgat aacagaacca
     7561 ttgagttttt caatttgcgt tttcacaaca tcaagaccaa caccacgtcc tgaaacattc
     7621 gacacctttg cagcagttga aaaaccagct tcaaagataa gttgatgtgc ctcatgatct
     7681 gataaaatct tatttggatg aattaagcct cgctcaattg ctttttgttt taccttttct
     7741 acgtcaatac cagcaccatc gtcttggata tcgattacaa tcatatttcc ttcgttgctg
     7801 gctttcaaaa gcactgttcc ttcttcgctc tttccttttt tagcacgttc ctctggccgt
     7861 tctataccgt ggtctattga atttcgaaca cagtgcataa taggatcgag tagatcttct
     7921 ataacagatt tgtcaagttc tgtatcttca ccctctattt caaggtgaat ttttttatcg
     7981 aggtcccgag acaaatctcg aataactcgt ggaaaacgag aaaaaatctg atttacagga
     8041 accatgcgta ttttcataac tccttcttgt aactcacctg ctatccgtcc aagattttgt
     8101 gaagcagaac gaagttttgt attagctcct ttaaacgaag aatcaaagct ctcaaaaata
     8161 gagaaaatat ctgcatactc ggtatttaac cgggtacgaa tgtcttttat agtatctccc
     8221 tcttttaatg tttctataaa acttggtagt ttatcaaaaa gcgagcgaat tttttctcta
     8281 tatagagctt gagtgctcgc aagttgtgtt tgtaaatcac ccatttgcgt agtaatttgg
     8341 ttaaaagatg ccttcgtaat aactgtttca ctgacaaggt tgagaagata atcaattcgt
     8401 tttgaatcaa cgcgtaaaat actgcttgtg ctcgtctgtt gccctgtcgg agctactttt
     8461 ttcttttttt ccagtgcatc tgtttcttct tgaatatgtt cattaaatgc agttacttct
     8521 ttggcgcttt gttttgcctc gatggacgca gctatttctt gtcgcgattc ttcgtatttt
     8581 gctgcagaag tgggagtggg cgtatcttta aaactttttt ctggactact aaaagtcgac
     8641 ttttgagaat caattttttt tgcatcaaca gccaaggtga catcatccat aaaggctgca
     8701 tcttcaagtt gctcagcatt cagtgcagag cttataaaat acacaacttt ttcatgaaat
     8761 tcgtcatcgt agaggacgtc aaaatcggga attgttttta aaacagcacc cttatctttt
     8821 aatgcagaga aaacttgaat accgccaacg gaattcattg gattcgattc atcaaaaata
     8881 accgttatac cccaaacagg atcatcgctt ggtctcgact cattgagctc tagcaaatca
     8941 tactcgctta aaagctcagc agggtcttgc atagtcggca acgcaggcga tgcggtatgc
     9001 tgaggagttt gaaaagaagc gagggattta ggtggtgtcg actttgtttt ttctcccttt
     9061 gcagggataa aggattttaa ggtttctttg agtggttcaa catcatcagt atacacacta
     9121 ccattagagc gatactgtaa catttccttg atgacgtcta ttgcattgag caaggtatca
     9181 acaacctctt cgcttacaga gatttttttt gcacgcaaga catcgagtaa atcctctaca
     9241 acatgagtga aacctgagag ttcatgcatt tcaaccgtgg cagaccctcc tttgagagtg
     9301 tgcgcagcac gaaaaatttc gtctatagcc tcagtgtttg aagggtctgt ctcaataaca
     9361 agaatattgc tttcaagagc ctgcacttgt tgttcagctt cactaaagaa gtcttttaaa
     9421 agttcttcat tatttaaatc aaggtaatca ctcatataca ttattttacc agttaatata
     9481 tataagtcaa gataaataga caataaaatg ctttcttttt ttatagcctc tattttcacc
     9541 cttcctattg aaaatatgaa aatctgccga tacactgtat aaggtagata cttttgtttt
     9601 ttggagtaaa cagtgaaaaa aaaatataat acgaagatac gacatatagt tctcgtcatt
     9661 gccctctttt ttgtaggttt ccaaggctat gcagccccat cttccaatgg aatcattggt
     9721 gctggtctac tcattcaacc tatgccacat gaactgcctg atctatttgc agaagctttt
     9781 ttcgcaagcc aaatagacct ttcccaaagc ctccttatac gcactgaatt ttcagttttt
     9841 accgattcca taattggtga taactttctc aaggatatcc ctgcatattt tagcttaaat
     9901 gaactttctc tcacatacgt agggagtctc ggtacaataa gtcaactcgc aagcgttttt
     9961 ataggagaaa cagacc
//
LOCUS       DAOSSW010000066         9937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_6588_length_9937_cov_8.149767, whole genome shotgun sequence.
ACCESSION   DAOSSW010000066 DAOSSW010000000
VERSION     DAOSSW010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6588_length_9937_cov_8.149767"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1073
                     /locus_tag="PKW26_06295"
     CDS             147..1073
                     /locus_tag="PKW26_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOQ93211.1"
                     /translation="MNWVLLSTSKMQMNKNLVLSYLQTKNSNVVLIELQKDILEMKSS
                     AEFQEKFEKICRDLERADFFVFFLCDQDSFDPFSLFLLGFARAKAIPLYFIGEKIEKI
                     EPHSFFDVFLYKNEENFLDSLDKSFLKIEEEKQSKNARQKICEMGLAFSVDGFSQAIR
                     DADTKICNVFLASELDFNGRDSLGTPLLNIAVRAENIDIVKKLLKKSVSIDAKSLDRG
                     YSALMDAVWKSNLALVQLLIAAGSDVNFISDDGQSILVLATGVDNYDICNALVKAGAS
                     CHLKDNMGMSALDYARLFKKEHLVALYEKGGV"
     gene            1070..2206
                     /locus_tag="PKW26_06300"
     CDS             1070..2206
                     /locus_tag="PKW26_06300"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOQ93212.1"
                     /translation="MMFCAFTGGGTAGHIYPGLAVVDELKALCPELSFLWIGSSKGMD
                     KHIVEESGSVDLFKGIASGKLRRYFSFKNFIDVFKIFFGFIASFFILLKYKPLFLFSK
                     GGFVSVPPCFAAKLLGIPVYTHECDFSPGLATKLNARFAKKIFLSYEETRTFFPKEKQ
                     NTLLVFGNPVRPIFYKTDAKLGFDFLFKENQELYETVKTKPLLLVLGGSLGARQINTL
                     VEENLEWLCEHCIVVHQRGKNALLAETSYKEYLPYEFIYSEMPHVLAAADIVLSRAGA
                     NSLSEALVLQKPMILIPLMGSGTRGDQVENAQNLEKNKAAQVLLAEDVHADSLKKSLL
                     FFLDKKNRTNYAENTKKMNSIEKPAQKIAEYLAREEVKGQCSMA"
     gene            2191..2664
                     /locus_tag="PKW26_06305"
     CDS             2191..2664
                     /locus_tag="PKW26_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOQ93213.1"
                     /translation="MLNGLDVFFILISLVLILLDTLKGFVSSFFNKAAFVLALLFAFL
                     FFSYGAFFYGQFIQIGFLSALLSFVSIFAIVFLIVKIFQSLTKKVFFSNPIMASLDRS
                     LGFLFGLVEAFVLIIFILYLLQAQNIFSVDSLLGNSFFYTLLQPILGFEPVLIKG"
     gene            2667..3884
                     /locus_tag="PKW26_06310"
     CDS             2667..3884
                     /locus_tag="PKW26_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HOQ93214.1"
                     /translation="MFENVLYQNAVDQLIQAIENKDLPQSLLFFGPSSSAKLTTALEL
                     ARVLSCKAAVKGEWLCSCSSCAQHKLLVHQNLLLLGPRETSLEIAASEASFLKAISQN
                     AEYKEATKFLFLRSIRKLLLRYHPVLLQDDDQASKVASHVLQINELLEELEQGLHTSE
                     IKDIQKITESLVDLSVKLEAQYSYDSIPVLHIRNLSYWARLTSSDGKKIVIIENADRM
                     LDASKNALLKILEEPPKNLTFILLTTNRNALLPTILSRLRPYYFQERKEEEQKEVIER
                     VFHLTASNIEDFLLGYLPVSLEIIQNNAKKLYAMLEGESLLEVEGIIKDLGGLKSRLV
                     LSLFFNQLFLLQRESIRNADEDKKALALEKTYEALSFIKTCYENIRFLHQNPQSAFES
                     LAHSLLKNKKNTL"
     gene            3948..5147
                     /locus_tag="PKW26_06315"
     CDS             3948..5147
                     /locus_tag="PKW26_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ93215.1"
                     /translation="MRNFVRRASQKISKLSNEQLTQLVESIYTENETLDSVIESLSIG
                     LLICDVDWKLLFANKASERFMPFTVRLSEFRSTDAVFDQEVWKFIADSDIAGFLEKNA
                     EKTYTSQDFTLETSGGNRRFVNISTMPLVREKKLIGNIIKIDDITEKKNAEVLLRRME
                     SLASLTNLAASVAHEIKNPLGSISIHIQLIQKIVQKARENKNLLLDNGKIEKYLDITT
                     EEIERLNKTIVDFLFAVRPISAHLELIDVHALLRHFLPFFQAELDEKKIRLKTDIPAG
                     EQKLLVDEKLFKQLLINLIQNAMDVLPAGGCIEISGQLENSSYVLFLKDNGRGIADED
                     LHKIFEPYFTTKITGTGLGLTMVYKIIKEFSGDIIVSSKLGEGTVFKMTFPLPQMDRK
                     YLDYTGE"
     gene            5149..6516
                     /locus_tag="PKW26_06320"
     CDS             5149..6516
                     /locus_tag="PKW26_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOQ93216.1"
                     /translation="MKFNILIIDDEKNIREGLAESMELEGYNPILAKDGKEGLERISR
                     GDIDLVITDLRMPLVSGEEVLEQVNAQSPGLPVIVLTGHGSIDSAVDAMRNGAYDFLT
                     KPLNLDRLGLIVKRALQNRQLVLQHRELQHELESKQTFESIIGKSAEMQKIFDMVRRV
                     ADSKASVLITGESGVGKELIANALHNLSSRKDKPLIKVHCAALTESLLESELFGHEKG
                     AFTGAISRKRGRFELAHGGSIFLDEIGEIDQNIQIKLLRVLQEKEFERVGGEETIQVD
                     SRVIAATNKDLVEQIKKKQFREDLYYRLNVVHIHLPPLRERKDDIPLMVTQFLQEFAE
                     ENNKNIIGIDAKARRLLYSYDWPGNIRQLRNCIESAVVMSQGDTIQVEDLPPTIAESR
                     GENSVHIPLGITMAEAEKVIILENLAFFKGNKSKTADSLGIGRKTIHRKLDDYLATDE
                     RESQE"
     gene            6609..9785
                     /gene="ileS"
                     /locus_tag="PKW26_06325"
     CDS             6609..9785
                     /gene="ileS"
                     /locus_tag="PKW26_06325"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOQ93217.1"
                     /translation="MYKPVDPKVDFSKEEEKILEFWKTNNIFKKSIEQRAENEEYVFY
                     DGPPFATGLPHFGHFIPSTIKDIIPRYQTMKGYKVERRFGWDCHGLPVENLIEKELGL
                     NSKTDIEKFGIAAFNEACRDSVLRYVKEWEQTITRLGRWVDFENDYKTMDPDYMESIW
                     WIMKQLYDKKLMYEGHYILPYCPRCATVLSNHELNLGGYKDVHDPAITVRFKVNGATK
                     EQLRFDKDLVNGNTYLLAWTTTPWTLPSNLGLTMGPEIDYVKVQDGDDFYILAKERLS
                     AYYKSEEEYRIVWEKKGTELLDLQYEPLFSYFASLKTNEDGSMGAFRVFNGDFVTTGD
                     GTGIVHTAPGFGEDDNRIFKGTGVPVVCPIDAECKFTEEVSDYTGRFVKDSDKDIIEQ
                     LRLEGKLVKRDQILHSYPHCWRCSSPLIYRAVGSWFVGVETFKDSLLKANAKIKWQPE
                     HIKAGRFGKWLEGARDWAISRNRYWGNPIPVWKCESCDNQICVGSRDELKELSGIYPE
                     DLHKHFVDEIDIPCSCGAQMHRIPEVLDCWFESGAMPYAQNHYPFENKEYFDSHFPAD
                     FISEGLDQTRGWFYTLTVLAAALFDKPAFKNCIVNGLVLASDGKKMSKSLRNYTDPAE
                     AVDQFGADAIRLFLMHSAVVKADDLKYSDDGVKDVLKSVLIPLWNSYSFFVTYANIDG
                     IQAEADFTHLPSNPLDRWLLSMTQKLIYDVTHAMEDYDLSKAIDPIVLFIDQLNNWYI
                     RRSRRRFWKSENDMDKTEAYNSLYYALKTFALVSAPFIPFVTETMYLNLRTQNEPESL
                     HLNDYPIYNEKMRDLDLEFKMDTVQKAVSMGRSLRYQFNLKIRQPLKSVEFVTKNPDE
                     KAVLLEMEESIREELNVKKVVFHEREDELVEYRAKANFKTLGKSLGPLMKAASVEIAK
                     LSADIIQSILDGSTISIEVGGKNIDLDESSIIVERIEKAQLKVVNEGTLTVALDSEIT
                     EDLQMEGMVRDLVRGIQNLRKESGFDVTDRIQLLVHGPEPLKKAFDAFSDYIASETLA
                     TKIEWQALSMSTSIEAGDTAWEVKIEKA"
BASE COUNT         3101 a         1758 c         2054 g         3024 t
ORIGIN      
        1 ctgtagctca gtcggtagag caatggactg aaaatccatg tgtcgacagt tcgattctgt
       61 ccggtggcaa aaactgcata cttctgtatg cagttttttt ttatttccct tgttttcagc
      121 tagaaaataa cgtatactgt ttatctatga attgggtttt actttctaca tcaaaaatgc
      181 aaatgaataa aaatctcgtc ctctcctact tgcaaacaaa aaatagcaat gtcgttttga
      241 tagaacttca aaaagatatt ttagagatga agtcttcagc tgaatttcaa gaaaaatttg
      301 aaaaaatttg cagagactta gaaagagctg atttttttgt tttttttcta tgcgatcaag
      361 attcttttga tcctttttct ctatttcttc ttggttttgc acgggcaaag gcaattcctt
      421 tgtattttat aggagaaaaa atagaaaaaa tagaacctca tagttttttt gatgtttttc
      481 tgtataaaaa tgaagaaaac tttctcgata gcttagataa gagcttcttg aaaatcgagg
      541 aagaaaaaca aagtaaaaat gcgagacaga aaatttgtga aatggggctt gcctttagcg
      601 tagatggttt ttcgcaagct attcgtgatg cagatacaaa gatctgcaat gtgtttttag
      661 catctgaact tgattttaat gggcgagatt ctttaggtac gcctcttctt aatatcgctg
      721 ttcgcgctga aaatatagat atcgtaaaaa aactcttaaa aaaatctgta tctatcgatg
      781 caaaatctct tgaccgaggc tattctgcat tgatggatgc ggtgtggaag tctaaccttg
      841 cattggtaca attgcttatt gctgcaggct ctgacgtaaa ttttatttcc gatgatggac
      901 agtctatcct tgtgttggca actggtgtgg ataattacga tatttgtaat gccttagtaa
      961 aagctggagc aagttgtcac ttaaaagata atatgggaat gtctgcgctt gattatgccc
     1021 gccttttcaa aaaagaacat cttgtcgcct tgtatgaaaa agggggagta tgatgttttg
     1081 tgcatttaca ggtggtggta ccgctgggca tatttaccct ggcttagcgg ttgtcgatga
     1141 attaaaggct ctttgcccag agctttcttt tttgtggatc ggctcttcta aaggaatgga
     1201 taaacacatt gtcgaagaat ctggatcggt cgaccttttc aagggaattg cttcgggaaa
     1261 attgcggcgc tatttttctt ttaagaactt tatagatgtt tttaaaatct tttttgggtt
     1321 tatcgcttcc ttttttatcc tcttaaaata caaaccgctt tttttatttt caaagggtgg
     1381 ctttgtctcc gttcctcctt gctttgcagc aaaacttttg ggtattcctg tatacactca
     1441 tgaatgtgat ttttcccctg ggctagcaac aaaactgaat gcgcggtttg caaaaaagat
     1501 ttttctttca tatgaagaga caaggacttt tttcccaaag gaaaagcaaa ataccttgct
     1561 tgtttttgga aatcctgttc gtcctatttt ttataaaacg gatgcaaaac tcggcttcga
     1621 ttttcttttt aaggaaaacc aggaattata tgaaacagta aaaacgaagc cattattatt
     1681 ggtgctggga ggaagcctgg gtgcgagaca aataaatacg cttgtcgaag aaaatcttga
     1741 atggctttgt gagcattgta ttgtagtgca tcaaagagga aaaaatgcgc ttttagcgga
     1801 aacctcatac aaagaatatc tgccatatga atttatctac agtgaaatgc cccatgtttt
     1861 ggctgctgcc gatatcgttc tttctcgagc aggtgcaaat agcttgagcg aagccttggt
     1921 tcttcaaaaa ccgatgattc tcattccgct catgggttct gggacgaggg gcgaccaagt
     1981 tgaaaatgcc caaaatctag aaaaaaataa ggcagcgcag gttttattgg ctgaagatgt
     2041 acatgctgac tctttgaaaa aatcgctttt gtttttttta gataaaaaaa atagaacaaa
     2101 ctatgctgaa aatacaaaga aaatgaattc tatagaaaaa cctgcacaga aaattgcaga
     2161 gtatcttgcg agagaagagg taaaaggcca atgctcaatg gcttagatgt tttttttatt
     2221 ttaatttctc ttgttcttat ccttttggat acgctaaaag gctttgtttc ctcctttttt
     2281 aacaaggctg cctttgtttt ggcgctttta ttcgcttttt tatttttttc ctatggggca
     2341 tttttttacg ggcagtttat tcagattggg tttctctcag cgcttctttc ctttgtatca
     2401 atctttgcta ttgtatttct catcgtaaaa atttttcaaa gtctaacaaa aaaagtgttt
     2461 ttttcaaatc cgattatggc aagcttagat cgctctctcg gctttttatt tgggcttgta
     2521 gaagcctttg ttcttattat ttttatcttg tatcttcttc aagcgcaaaa cattttttct
     2581 gtagacagtt tactgggaaa tagttttttt tatacgctct tgcaaccgat tttgggcttc
     2641 gagcctgttt taattaaagg atagttatgt ttgaaaatgt attatatcaa aatgcggtcg
     2701 atcagctcat acaggcaatt gaaaataaag acttaccgca gtcgctttta ttttttggac
     2761 cctcatcgag tgcaaaactt acaacagctt tagagcttgc gcgagtactt tcttgcaagg
     2821 ctgcggtaaa aggggagtgg ctctgtagct gttcttcttg tgcgcagcat aaactgctcg
     2881 tgcatcaaaa tcttttattg cttgggccac gggaaacgag cttagagatt gcggcgagtg
     2941 aagcaagttt tttgaaagcg atttctcaaa atgctgagta taaagaggca acaaagtttt
     3001 tgtttttgcg gagtattcga aagctcttat tgcgctacca tcctgttttg ttacaagatg
     3061 atgaccaagc ttcgaaagtt gcctcgcatg tcttgcaaat aaatgaattg cttgaggaat
     3121 tggaacaagg tcttcatacg agtgaaataa aagatatcca aaaaattaca gaaagccttg
     3181 tagacctttc tgtaaaactt gaagcacaat atagttatga tagtattcca gttttgcata
     3241 ttcgaaatct ttcctactgg gcacgtctga caagttctga tggaaaaaaa attgttataa
     3301 tagaaaatgc cgatcggatg ctcgatgctt caaaaaatgc tttgttaaaa attttagaag
     3361 agccgccaaa gaatcttacg tttattttgc tgacaacaaa tagaaatgcg ctcctaccga
     3421 caattctttc aagacttcgc ccctattatt tccaagaaag aaaggaagaa gagcaaaaag
     3481 aggtgataga gcgagttttt catttgaccg catcaaatat cgaagatttt ttacttggct
     3541 acttgcccgt ttctcttgaa ataattcaga ataatgcaaa aaaactctat gctatgctcg
     3601 aaggagaaag tcttcttgaa gttgaaggaa ttataaaaga cttaggcggc ttaaaatcgc
     3661 gccttgtttt atccttgttt tttaatcaat tatttttatt gcagcgtgaa tctattcgca
     3721 atgcagatga ggataaaaag gccttggcgc ttgaaaaaac atatgaggcg ctttctttta
     3781 taaaaacttg ctatgaaaat atacgttttt tacatcaaaa tccacagtcg gcttttgaat
     3841 ccttggctca ttctttacta aaaaacaaaa aaaatacttt gtaagcccta ggatttttac
     3901 tttgtttttt aaattttagt cggcagaaaa atacaaaagg taggtctatg cgaaattttg
     3961 tcagaagagc ttctcaaaaa atttcaaaac tttcaaacga gcaactcact caactggttg
     4021 aaagtatcta tactgaaaac gaaaccttgg attctgttat agaatcttta agtattggtt
     4081 tgcttatatg cgatgttgac tggaaattgc tttttgcaaa caaggcttct gagcgtttta
     4141 tgccctttac ggttcgcctt tctgagtttc gctctacaga tgcagtcttc gatcaagagg
     4201 tgtggaaatt tatcgccgac tccgatattg caggtttttt ggaaaaaaat gccgaaaaaa
     4261 catatacgag tcaagatttt accctggaaa cgagtggtgg taacaggcgt tttgtaaata
     4321 ttagcactat gcccttggtg agggaaaaaa aactcatcgg caatattata aaaatcgatg
     4381 atataactga aaagaaaaac gcggaagttt tactgcgccg tatggaaagt cttgcaagtt
     4441 taaccaatct tgctgcaagc gttgctcatg agataaaaaa tcctttggga agcattagta
     4501 ttcatataca acttatacaa aaaattgtac aaaaggcacg agaaaataaa aatcttcttt
     4561 tggacaatgg gaaaatcgaa aaatatctcg atataacaac tgaggaaata gagcgtttaa
     4621 ataaaactat tgtcgatttt ttatttgcgg tgcgtcctat cagtgcccat cttgaactca
     4681 tcgatgttca tgcactttta cggcattttc ttccattttt ccaagcagaa ctcgatgaga
     4741 aaaaaatacg tttaaaaaca gatattccag cgggtgagca aaaactgctc gtcgatgaaa
     4801 aactatttaa acaattgctt atcaatctta tacaaaatgc tatggatgtt ttacctgcag
     4861 gtggttgtat agagatttct gggcagcttg aaaatagctc ttatgtactt tttcttaaag
     4921 acaatggaag gggaatcgca gatgaagacc ttcacaagat ttttgagcca tacttcacaa
     4981 caaagataac gggtacaggg cttggtttaa cgatggtcta taaaataata aaagagtttt
     5041 ctggcgatat tatcgtttca tcaaagcttg gcgaaggaac cgtttttaaa atgaccttcc
     5101 cacttccaca aatggacaga aaatatttgg attatacagg ggaataaaat gaagtttaat
     5161 attttaataa tagatgatga aaaaaatata cgagaagggc ttgcagaatc catggaactt
     5221 gagggctata atcctatttt ggcaaaagac ggaaaggagg gcttagagcg tatttctcgt
     5281 ggcgatatag accttgttat aactgattta cgcatgccgc tggtaagcgg tgaagaagtt
     5341 ctggagcagg tgaatgcaca aagccccggc ttgccagtga ttgttttaac aggtcacggt
     5401 agtattgact ccgcagtcga tgctatgcga aatggtgctt acgatttttt aacaaagccc
     5461 ctcaatctcg atagactcgg cctcatcgta aaacgcgcac ttcaaaatcg ccagctcgtc
     5521 ttacaacatc gagaactgca acacgaactt gaaagtaaac aaacctttga gtccattatc
     5581 gggaaaagtg ctgaaatgca aaaaatcttt gatatggtgc gacgagttgc agattccaag
     5641 gcttctgttt tgataactgg tgagagcggg gtaggaaagg agcttattgc aaatgcgctg
     5701 cataatcttt caagcagaaa agacaagccc cttatcaagg tgcattgtgc cgcattgaca
     5761 gaatcgcttt tggagagtga actttttggt catgaaaaag gtgcctttac gggtgctatt
     5821 tctagaaaac gtggacgctt tgagcttgcc catggtggta gtatttttct agatgaaata
     5881 ggtgaaatcg atcaaaatat tcaaataaag cttttgcggg ttttgcaaga aaaagagttt
     5941 gagcgagtcg gtggcgaaga aaccatacag gtagatagcc gtgttatcgc agcgacaaat
     6001 aaagacttgg tcgagcagat aaaaaagaaa caattccgcg aagatttgta ttatcgatta
     6061 aatgtcgttc atattcatct gccgccgctt cgtgagagaa aagacgatat tccgcttatg
     6121 gttacccagt ttttgcaaga gtttgcagaa gaaaacaata aaaatatcat cggtatcgat
     6181 gcgaaggcac gaaggctttt gtatagctat gactggccgg gcaatattcg tcaactgcgt
     6241 aactgcatag aaagcgctgt tgttatgagc cagggcgata ccatacaggt agaagactta
     6301 ccgccgacta tagcagaatc tcggggtgaa aactccgtgc atattccctt gggaattact
     6361 atggccgagg ctgaaaaagt aattattttg gaaaatcttg cgttttttaa aggaaataaa
     6421 tctaaaactg cagattcact tggaattgga agaaaaacaa ttcatcgtaa actcgatgac
     6481 tatctcgcta ctgatgaaag agaaagccaa gaataaatct tatcttgaag aaatattttt
     6541 tttatgtcat actaatcgga aaagaatagg tgattataga aaatcacagc agaaaaaagg
     6601 ggattatttt gtataaacca gttgatccaa aggtggattt ttcaaaagaa gaagagaaaa
     6661 ttttagagtt ttggaaaaca aacaatatat ttaaaaaatc aatagagcaa agagcagaaa
     6721 acgaagaata tgttttttat gacggacctc cctttgcaac aggtttacca cactttggac
     6781 attttatacc aagtaccata aaagatatca taccccgcta tcaaactatg aagggctata
     6841 aggttgagcg ccgttttggc tgggattgtc atggtttacc agtagaaaat ctcatagaaa
     6901 aagaactcgg gctcaattct aaaaccgata tagaaaaatt tggcatcgct gcctttaatg
     6961 aagcttgtcg cgactccgtt ttgcgctatg taaaagaatg ggagcaaaca ataacacggc
     7021 ttggtcgctg ggttgatttt gaaaacgact ataaaaccat ggaccccgat tatatggagt
     7081 ccatttggtg gataatgaaa caattatacg ataaaaaact catgtacgag gggcattata
     7141 ttttacccta ttgccctcgt tgtgcaacag ttttatcaaa tcatgaatta aatctcggtg
     7201 gctataagga tgtgcacgac cctgcgatta cggtgcgctt taaagtaaat ggagcgacaa
     7261 aagagcaact gcgttttgac aaagacttag taaacggaaa cacctattta ctggcatgga
     7321 caacaacccc ttggacacta ccctccaacc ttgggcttac tatgggtcct gagattgact
     7381 atgtaaaagt acaagatggg gatgattttt atatcttagc gaaagaacgt ctatctgcgt
     7441 actataaaag cgaagaagaa tatcgcattg tatgggaaaa gaaggggact gaactcctag
     7501 acttgcaata cgaaccgctt ttttcatatt ttgcttcact aaaaacaaac gaagatggct
     7561 caatgggagc atttcgtgtg tttaacggcg actttgtgac taccggtgat ggtaccggca
     7621 ttgtacatac cgcacctggc tttggtgaag atgacaatag aattttcaaa ggaacaggtg
     7681 ttcccgttgt ttgccccata gatgctgaat gtaagtttac tgaagaagtt tcagattata
     7741 ctggtcgctt tgtaaaagac agcgacaagg atataatcga acaattgcgc ttagaaggca
     7801 aactcgtaaa gcgagatcaa atcttgcact cctatccgca ttgctggcgt tgttcaagtc
     7861 cccttattta ccgagctgtt ggttcatggt ttgttggggt agaaactttt aaagactccc
     7921 ttctcaaggc aaatgcaaag attaagtggc aaccagaaca tataaaggcc ggtcgatttg
     7981 gtaaatggct cgaaggggca agagactggg caattagccg aaaccgctat tggggaaatc
     8041 ccattcctgt atggaaatgt gagtcctgcg ataatcaaat ctgcgtagga agtcgtgatg
     8101 agctaaaaga gctctccggt atctatcccg aagacttaca caagcatttt gtcgatgaga
     8161 tagatattcc ttgttcttgt ggcgctcaga tgcatcgtat tccagaagtt ttagactgct
     8221 ggtttgagtc aggtgcaatg ccctatgcgc aaaatcatta tccatttgaa aacaaagaat
     8281 attttgacag ccattttccc gcagacttta tttcagaagg cttagaccaa acacgcggct
     8341 ggttttatac cctcactgtt ttggctgcag ccctctttga taaacccgct tttaaaaact
     8401 gcattgtaaa cgggcttgtt cttgcctccg atggcaaaaa aatgtcaaaa agtttacgaa
     8461 attatacaga cccagcagag gctgttgacc agtttggtgc agatgcaatc cgtctttttt
     8521 taatgcactc agctgtagta aaggcagatg acctcaaata cagcgatgat ggtgtaaaag
     8581 atgtgttaaa aagcgtttta attcccttgt ggaatagcta tagttttttt gtaacctatg
     8641 caaatatcga tggcatacaa gcagaggctg attttacgca tcttccttca aatccactcg
     8701 accgctggct tttatctatg acacaaaaac tcatttacga tgttactcat gcaatggaag
     8761 actacgacct ttcaaaggca atcgacccca ttgttttatt tatcgaccag ttaaataatt
     8821 ggtatattcg cagaagtcgc agacgatttt ggaaaagcga aaacgatatg gataaaaccg
     8881 aagcatacaa tagcttgtat tatgcgctta aaacctttgc cttagtaagt gcgcctttta
     8941 tcccctttgt tacagaaaca atgtacttga acttgcgcac gcaaaatgaa cctgaatcac
     9001 tgcacttaaa tgactatccc atctacaacg aaaagatgcg agatttagac cttgagttta
     9061 aaatggatac agtgcaaaaa gcggtttcaa tgggacgttc attacgctat caatttaact
     9121 taaaaatccg tcaaccgcta aagagcgttg agtttgttac gaaaaaccca gatgaaaaag
     9181 cggtgctttt agaaatggaa gaaagcattc gtgaagagct caatgtaaaa aaagtggtct
     9241 ttcatgaacg agaggatgaa cttgtcgagt acagggcaaa ggcaaacttc aaaactttgg
     9301 gcaaaagtct tggtcccttg atgaaggctg cctcagttga aattgcaaag ctcagtgccg
     9361 atattattca atctatctta gatggttcta cgattagcat agaagttggt gggaaaaaca
     9421 ttgacttaga tgagtcttcg attatcgttg aaagaattga aaaggcacaa ttaaaagttg
     9481 tcaatgaagg cacgctcacc gttgccctcg actctgaaat aacagaagac ttgcaaatgg
     9541 aaggtatggt tcgagacttg gtgcgcggca ttcaaaacct tcgtaaagaa agcggcttcg
     9601 acgttaccga cagaatacaa cttttagtgc atggaccaga gccattaaaa aaagcctttg
     9661 atgcgttttc tgactatatt gcgagtgaaa cacttgcaac aaagatagaa tggcaagcgc
     9721 tttcaatgag cacttcgata gaagcaggag atactgcctg ggaggtaaaa attgaaaaag
     9781 cctaggatct gtttttttac agtcctactt attctctttt tacttaatgc actttttttt
     9841 agttgtaaaa ctatttctac aaaaatcccc caagacccat ttatggttct cggggattct
     9901 ttttctctct atacagccct cgatgtgaaa cgagata
//
LOCUS       DAOSSW010000067         9888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_6619_length_9888_cov_6.743008, whole genome shotgun sequence.
ACCESSION   DAOSSW010000067 DAOSSW010000000
VERSION     DAOSSW010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9888
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6619_length_9888_cov_6.743008"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PKW26_06330"
     CDS             <1..281
                     /locus_tag="PKW26_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93218.1"
                     /translation="AETLLSRISQDEIQRIKIMREELAEVDYYSGLRAAEEQGLARGR
                     SEGLEQGLEQGLEQGRAQEKMNTARSLKARNFSTEDILEITGLSEGEY"
     gene            complement(329..631)
                     /locus_tag="PKW26_06335"
     CDS             complement(329..631)
                     /locus_tag="PKW26_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOQ93219.1"
                     /translation="MAYKIVFIPEAVLDYKALDGSIKKSVKAKIDALKENPFLGLQLG
                     NKQNIDLSGFYKLYVHKKKYRIVYRIISPEKIEILEIWGIGKRDKAEIYKLIGSRL"
     gene            complement(631..903)
                     /locus_tag="PKW26_06340"
     CDS             complement(631..903)
                     /locus_tag="PKW26_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOQ93220.1"
                     /translation="MLVNSDKIISITKLQRELTQKLREVTESGESVYIMKNNTMEAVM
                     MPIAEYAHLKKLDELFEQLEIQAMLKKRMKTYNPDKVISWEDIQDV"
     gene            1136..1582
                     /locus_tag="PKW26_06345"
     CDS             1136..1582
                     /locus_tag="PKW26_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOQ93221.1"
                     /translation="MNLRTVLTEDTVNLHLKGTTKEEIIDEMLDILVQAGKVTDKKAA
                     RECVLDRERKMSTGMKHGIAIPHGKTDTVDDLVACIGISDTPVDFDSLDQEPCRIFIM
                     TLSPVNKTGPHLQFLAEISLLFKSAEKRQEILETKDKAEVIRILTE"
     gene            1669..2388
                     /locus_tag="PKW26_06350"
     CDS             1669..2388
                     /locus_tag="PKW26_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93222.1"
                     /translation="MKENSKSYYPVLEDVSDYAEMEKQCQVLEKRTVLWILPWNAVSL
                     NEADTYDEAYLAHVKTIFSIAEGYSLQILLTPELTLFSLPSWVMQELNRVKLNEESIR
                     FECPYQSRNETDQSCLFTFFLALFFLGNDLFPEIKHEGESIQDFLQEQCIFAMKHAAR
                     RLKKNTNIEGFYFSKMLSEDFLYSYIKNIQNIELKNNERSEKLVISPELIKTKVDDFK
                     LCFKNEIIKKHDHFVFKSDTN"
     gene            complement(2471..3238)
                     /locus_tag="PKW26_06355"
     CDS             complement(2471..3238)
                     /locus_tag="PKW26_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOQ93223.1"
                     /translation="MSKKNNRLPLLLKTFFAPTVFSLFLAILCMYVLFYMPANSAEEL
                     TNEIARLEVSEEEQAALPVIAVDYHNLVINHKQKPDLGLSLYRNLESQAALIDFYTQI
                     TKNKTVTFAIVENADKNDIPLSLAFALAYTESNYKTSAVNKNKNTSIDRGLFQLNNNS
                     FPKLSESEFFDPYISAKYGLAHLRFCLDTAGNEVAALAMYNAGTNRVRNNGTPQMTLN
                     YVDKIFNYKEGLDALLYSELYDFTDNNQNRYIAMTSK"
     gene            complement(3381..4568)
                     /locus_tag="PKW26_06360"
     CDS             complement(3381..4568)
                     /locus_tag="PKW26_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOQ93224.1"
                     /translation="MAYKIQTLNKISGTGLEQFPRDNYEIASEILNPDAILLRSFDMH
                     EMEVPATVKAVARAGAGTNNIPIAKMSEKGIVVFNTPGANANAVKELAIAALLFSSRP
                     VIAAHKWVEALAGKGEEIPELAEKGKSQFVGPELLGKTLGVIGLGAIGVMVANTAVEL
                     GMNVIGFDPFISVDSAWSLSRAVQKAESLEALLAKSDYLSIHVPQTNETKGFINASKI
                     ASMKKGVRILNFARGGLVVNTDLLAGIESGKIACFVTDFADEDLLGNDKIICLPHLGA
                     STPEAEENCAYMAVKQIRDFLENGNIVNSVNFPQTKLEEAIPVGGARLCIANKNVPNM
                     VGQITTILAAEKINISGMVNKNRADLAYNLIDVESCLSQALVDKLRNIEGVIDVRLIS
                     NCQ"
     gene            complement(4584..5672)
                     /gene="serC"
                     /locus_tag="PKW26_06365"
     CDS             complement(4584..5672)
                     /gene="serC"
                     /locus_tag="PKW26_06365"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOQ93225.1"
                     /translation="MNRVWNFSAGPSVLPVEALREAGDEILNYNETGQSVMEMSHRSK
                     AFEPIIAETEALVRELMQIPENYKVLFLQGGASQQFAMVPMNLKNKGKAAFIDTGVWS
                     KKAISEAKKYLDVEVLASSKDKNYSYIPQLEKIEGDYDYVHITLNNTIMGTKWDYLPD
                     TGAIPLVADVSSNILSEPLDITKFGLIYAGAQKNLAPAGLTLVIIREDLVPETGIPGT
                     PTMLEYKTHVDNGSMYNTPPCWAIYMMGKVLKWVKKNGGAEGMKKRNNEKADLFYNYL
                     DTSDFYKATAEKGSRSLMNIPFLTKYTGDEADAVNKKFVKEAEAAGLYNLAGHRLVGG
                     MRASIYNAMDIEGVKALIAFMDKFAKNN"
     gene            complement(5689..6309)
                     /locus_tag="PKW26_06370"
     CDS             complement(5689..6309)
                     /locus_tag="PKW26_06370"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOQ93226.1"
                     /translation="MKKQISKPARERLIQLLRLLDQLEGSGEKTLITSGEIEMLTGWT
                     DATVRRDISLLSANSATSSGYSIATLRTAIEDALSINRGERACCIVGLGRLGSALLEY
                     EGLSSSSFVLKAGFDISKNRCETLRSSIPLYSTLRMERVIRDERIEFAILAVPEAVAQ
                     EVVDKLCEYGIKGIVNFTSRVLQVQETVSVQSLSVLDALQNLSSIV"
     gene            complement(6449..6757)
                     /locus_tag="PKW26_06375"
     CDS             complement(6449..6757)
                     /locus_tag="PKW26_06375"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOQ93227.1"
                     /translation="MPAILFSPEAKKDLHDIKKYISEELESPTAAQNTVSKIIKAIRN
                     LQDFPSSGTPLSSIIDIETQYRFIVSGNYLVFYKEDTPAVRIIRILYGKRDYLNILFS
                     "
     gene            complement(6757..7005)
                     /locus_tag="PKW26_06380"
     CDS             complement(6757..7005)
                     /locus_tag="PKW26_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOQ93228.1"
                     /translation="MANIKPVSDLRSYNIVLKDVAVGEPVFLTKNGRGKYALMDIEDY
                     RKTQATLKLFADLAKGEVVAEQGGWIKLEEIEEDLGIK"
     assembly_gap    7065..7164
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7206..7685
                     /locus_tag="PKW26_06385"
     CDS             7206..7685
                     /locus_tag="PKW26_06385"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOQ93229.1"
                     /translation="MFYYSYETPIGILGIEENEGFITRLDFLKDKNEGPAQKTIAETP
                     LIKKAYEQLTEYFQGERRVFELPLKPMGTDFQKKVWEALIAIDYGKTKTYKAIAERVE
                     SPKAFRAVGGACNKNPIAIFIPCHRVVGANGSLVGFAGGLSTKDFLLKLEDTQNHLF"
     gene            7793..9817
                     /locus_tag="PKW26_06390"
     CDS             7793..9817
                     /locus_tag="PKW26_06390"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOQ93230.1"
                     /translation="MIDTKRPIFPKKAVVTGGMPYGNKRLHLGHIGAVFIPADIYSRF
                     LRDRIGKENVVFVSGTDCYGSPIVEYYKKAIADGSFSGSLEDFVLSNHKAQKNELDMY
                     SISNNLFATSALGRSGEIHRELSAEVLKTLHKNGHLEKHVRPQFYDAKLKAFLNGRQV
                     IGRCPIPGCKSEKAYADECELGHPYEPKDLIAPKSALSGEAPEMKDVTNWYINLASFA
                     DEFDKWITWLEKDPGTRSFITATIKEFLAKPTLYVRQEHEDELEAIRPLLPPHRIKED
                     PSKAIPLEFNSLSECDKAGEILFEKNIRFRAGKTLTPFRLTGNIEWSIQAPTIDGVEG
                     LTFWVWPESLWAPISFTKTYLESIGKDADEWKKYWCSKDALVYQFIGEDNIYFYSLAQ
                     EAIFMALQGEKPEAFPADGFLQATQLIANKHLLQGKKKASSSGDEKPIMAEDLLKYYT
                     SDQLRAHFFALGLGLRSISFNPKPLNPDANPRESDAVLKEGMLLSNVTNRLARSCFYT
                     SQKYFDGKLPEGSVSDAIKKACDTAILEYERLMYTHEFHAIMMLLDTFIRDASKFWAS
                     ESKEATQKAEKKVGEHASIEEKMQAEADYMKSVLVDSFHYLRTIIALLHPIAPVGSQK
                     VFEYLKLDESFWSWDTIFEPLHFFIDESHVFKFLEPRVDFFEKHETQVGK"
BASE COUNT         2976 a         1989 c         1804 g         3019 t
ORIGIN      
        1 tggctgaaac cttattgtca agaatttctc aagacgaaat tcaaagaata aaaatcatgc
       61 gtgaagagct ggcagaagtt gattattact caggattacg cgctgctgaa gaacaagggc
      121 ttgcacgagg tcgaagcgaa ggactagagc aaggcttaga gcaaggttta gaacaaggta
      181 gagcacaaga aaaaatgaat accgctcgga gtttaaaggc tcgcaatttt tccacggaag
      241 atattctcga aataacaggt ctctccgaag gcgaatatta gtttaaactc ttacaaaaag
      301 cgcgaaactg ttcgagtcaa caagattttt acagtctgct tcctatgagt ttatatattt
      361 cggctttatc tcgttttcca ataccccata tttcaaggat ttctattttc tctgggctta
      421 taattctata cacaatgcga tatttctttt tgtgtacata tagtttataa aagccgctta
      481 aatctatatt ctgtttgttt ccaagttgga gacctaaaaa aggattttct tttaatgcat
      541 caatttttgc ctttacgctt tttttaatgc ttccgtccaa tgccttataa tctaaaacag
      601 cttcgggaat aaatacaatt ttgtatgcca ttatacatcc tgtatatctt cccaagaaat
      661 cactttatct ggattatatg ttttcatacg ctttttgagc attgcctgta tttcaagctg
      721 ttcaaacagt tcatccaatt tttttagatg cgcatattca gcaatgggca tcataactgc
      781 ttccatggta ttatttttca ttatataaac agattcgcca ctttctgtaa cttctcgcag
      841 tttttgagtg agctctcttt gtagctttgt aatcgaaata attttgtcgg aattaactaa
      901 cattttttcc tccattgcat ttaactctgt tttttagcac atatccaatc tagtcagcat
      961 atgtattata acatgtagaa tattatgtag caagatagat acgttccttt tttcgtccat
     1021 gaaaaaagta acgtacctgt ctacgaaatc agctttccga gaaaaacatg ctttttccga
     1081 agtttcgctt tttttttgct tgacttttac cctaactagc cttaatatat tgagtatgaa
     1141 cttacgaaca gtattaacag aggacacagt aaaccttcac ttgaagggta ctacgaaaga
     1201 agaaattatc gacgaaatgc ttgatattct cgttcaagca ggaaaggtga ccgataaaaa
     1261 agcagctcgc gaatgcgttc ttgatcgcga aagaaaaatg tcaacaggaa tgaagcacgg
     1321 tattgctatt ccacacggaa aaactgatac cgtagatgac ttagttgctt gtattggaat
     1381 atccgatacc cctgtagatt ttgactcctt ggaccaagag ccttgtagaa tttttataat
     1441 gaccctctct ccagtaaata aaactggacc gcacttacag tttttagctg aaataagtct
     1501 cttatttaaa agtgcagaaa agcgtcaaga aattcttgaa accaaggaca aagcagaagt
     1561 tattcgcatt ttaacggaat aatttaagca caagctcttt ttctgaatta aaggccactc
     1621 tataaaagtg gtcttatttt gtttagcctg aatagtaaaa ggataaaaat gaaagaaaac
     1681 agtaaaagct attatcctgt attggaagat gtatctgact atgcagagat ggaaaagcaa
     1741 tgccaagtac ttgaaaaaag gacagtgctt tggattttgc cttggaatgc ggtaagcctt
     1801 aacgaagctg acacatatga cgaagcatat ttagcacatg ttaaaactat tttttctata
     1861 gcagaaggct attcactaca aatactactc acaccagaac taactctttt ctccctgccc
     1921 tcttgggtaa tgcaagaact caatcgcgta aagctaaacg aagaatctat acgttttgaa
     1981 tgtccctatc aatcaagaaa tgaaacagat caaagctgtt tgtttacctt ctttcttgca
     2041 cttttttttc taggtaatga ccttttccct gaaataaaac acgaaggtga aagtatccaa
     2101 gattttttac aagaacaatg tatttttgcg atgaaacatg cagcgcgacg acttaaaaaa
     2161 aatacaaaca tcgaaggttt ttatttttcc aaaatgcttt ctgaggactt tctctattct
     2221 tacataaaaa atatacaaaa catcgagtta aaaaataacg aaagaagcga aaaactcgtt
     2281 atcagtcccg aattgataaa aaccaaagtc gatgatttca aactatgctt taaaaatgaa
     2341 ataataaaaa aacacgacca ttttgtcttt aaaagtgata caaattaaac ggaagcacaa
     2401 tattgcctag gttttcactc aaaatcgaaa aaaagctgct attattttag cagctttttt
     2461 ttatagcttt ttattttgaa gtcattgcta tataacgatt ttggttattg tctgtaaaat
     2521 cataaagttc agaatagagc aaggcatcta aaccttcttt gtaattaaag attttatcaa
     2581 catagtttaa ggtcatttgc ggtgtcccgt tgttgcgcac gcgatttgtt cctgcattgt
     2641 acattgcaag ggctgctact tcatttccag cggtatctag acaaaaacgt aaatgggcta
     2701 agccatattt tgcgcttata tagggatcaa aaaactcact ttcgcttaat ttcggaaagg
     2761 aattattatt taactgaaat aatccgcgat ctatcgatgt gtttttgttt ttattgacag
     2821 cgcttgtttt ataattgctt tcagtatagg ccagggcaaa tgccaaagag agcggaatat
     2881 catttttatc ggcattttct acaattgcaa aggttacggt tttgtttttt gtaatctgcg
     2941 tataaaagtc aataagtgca gcctgtgatt ctaaatttcg atataaactc aaaccaaggt
     3001 caggtttttg tttatggttt ataaccaaat tatgataatc aactgctata acaggaagtg
     3061 cagcttgttc ttcttcgctt acttctaggc gagctatttc gtttgttagc tcttcggcgc
     3121 tatttgcggg catataaaat aatacgtaca tacataagat tgccaagaaa agtgaaaaaa
     3181 cggtcggagc aaaaaaggtt ttaagtaaaa gaggcaatct attatttttc ttactcattt
     3241 aaaaaactcc aaggaaataa tatcgcttag tatgccatac tatttccttg tgttcaatgc
     3301 cttaggtcgc ttttaagcga aagctttgct ttatttcgca aataatacca ttcaaaagcg
     3361 gttctttcta gattttagcc ttattgacag tttgatatga gccgaacatc aataacacct
     3421 tcgatatttc gtagtttatc gacgagggct tggcttaagc aagattcaac atcgatgagg
     3481 ttatacgcaa ggtcggcacg atttttattt accataccag aaatatttat cttttctgca
     3541 gcaagtattg ttgtaatttg cccaaccata ttcggtacgt ttttatttgc aatgcagagt
     3601 cgcgcgcctc ccactggaat agcttcttca agctttgttt gcgggaaatt taccgaattg
     3661 acgatattgc cgttttcaag aaagtcgcgt atttgcttta ctgccatgta agcgcagttt
     3721 tcttcagctt ctggtgtaga tgccccaaga tgtggtaggc agataatttt atcgtttcca
     3781 agcaaatctt catcagcaaa gtcagttaca aagcaggcta tttttccgct ttcaatgcca
     3841 gcaagtaagt ctgtgtttac aacaagaccg ccacgagcaa agtttaatat acgaacaccc
     3901 tttttcattg aagcaatttt acttgcattg ataaagccct tcgtttcgtt tgtttgtggt
     3961 acatgaatgc ttaaataatc tgactttgca agaagggctt ctaggctctc cgccttttgt
     4021 accgcccgac tcaaggacca ggctgaatcg actgaaataa aggggtcaaa gcctataacg
     4081 ttcatgccaa gttcaacagc ggtatttgca accatcacgc cgattgcacc caaaccgatg
     4141 acgccaagtg tttttcccaa gagttcagga ccaacaaact ggctttttcc tttttctgcc
     4201 aattctggaa tttcctctcc cttgcctgca agtgcttcta cccatttatg tgctgcaatc
     4261 actggtcgac ttgaaaataa aagcgctgca attgccaact cttttaccgc atttgcattt
     4321 gctcccggtg tgttaaatac cacaataccc ttttcgctca ttttcgcgat ggggatattg
     4381 tttgttccag ctcctgcacg agcaacagcc tttaccgtag caggtacttc catttcgtgc
     4441 atatcaaaag aacgaagtaa aatagcatcg ggattgagga tttcagaagc tatttcatag
     4501 ttgtcacggg gaaattgttc taaacccgtt ccagatattt tatttaatgt ttgaatttta
     4561 tatgccatac tatcttcctt gtattagtta ttctttgcaa acttgtccat aaaagcaatg
     4621 agtgccttta cgccttctat atccattgca ttatagatac ttgccctcat gccaccaacg
     4681 agtcgatgcc cggcgagatt atacaaacct gcagcctcag cctcttttac aaatttttta
     4741 tttaccgcat cagcttcatc gccagtatat tttgttaaaa aaggaatatt catcaaagag
     4801 cggctcccct tttctgctgt tgccttataa aaatcacttg tgtcgagata gttataaaac
     4861 aaatcagctt tttcgttatt gcgctttttc attccctcag caccaccgtt tttctttacc
     4921 cacttgagta ccttgcccat catataaata gcccagcaag gtggcgtatt gtacatggag
     4981 ccattgtcaa catgggtttt gtattcaagc atagttggtg ttccaggaat tcctgtttcg
     5041 ggtactaaat cctctctgat aatcacaagc gtaaggccgg caggggctaa gtttttttgg
     5101 gctcccgcat aaatgagacc aaattttgtt atatcaagcg gttcagataa aatattgctc
     5161 gatacgtctg caacaagcgg aatagctccc gtgtctggaa gatagtccca ctttgttccc
     5221 ataatagtat tgtttaaggt aatatgcaca tagtcatagt cgccttcaat tttttcaagc
     5281 tgtggaatat agctataatt tttgtcttta gaggaggcaa ggacttcaac gtcgaggtat
     5341 ttttttgcct cagaaatagc ttttttagac caaacacctg tgtcaataaa agcagccttg
     5401 cccttgtttt ttaggttcat aggaaccata gcaaattgct ggcttgcccc accttgtaaa
     5461 aaaagcacct tataattttc agggatttgc ataagttcgc gaacgagagc ctctgtttcg
     5521 gcaataattg gctcaaaagc ctttgagcga tggctcattt ccattacaga ttgtcccgtt
     5581 tcattgtaat ttaaaatttc atccccagct tctctaaggg cctctactgg cagaacggaa
     5641 ggtcctgcag aaaaattcca tactctgttc atcatttttg ttagtctcct atacgattga
     5701 acttaaattt tgtaaagcat cgagcaccga aaggctttgc accgacacag tctcttgcac
     5761 ttgcaaaaca cgcgatgtaa aattcacaat gccttttatg ccatactcac aaagtttgtc
     5821 aacaacttct tgggcaacag cttctggtac cgcaagaata gcaaattcaa ttctctcatc
     5881 ccgtataacg cgctccattc tcaaggttga atacaatgga atagatgagc gcaaggtttc
     5941 gcagcgattt ttgcttatat caaagcccgc cttgagaaca aatgaagaag agcttaaacc
     6001 ttcgtattcg agcaaggcag aaccaagccg cccaagtccg acaatacaac aggctctttc
     6061 ccctcgattg atgctcaagg catcttcaat tgctgttcgc agcgttgcta tgctatagcc
     6121 actgctcgtt gcagaatttg ccgaaagcaa agaaatatcc cgacgaacag ttgcatctgt
     6181 ccagccggtc agcatttcaa tttcgcccga tgtgataaga gttttttcac cgcttccctc
     6241 taattgatcg agaagacgca ataactgtat caatcgctcg cgcgctggtt ttgaaatctg
     6301 ttttttcatc tctttgctca cctagactgt gaattatttc acaacctaaa gatagaataa
     6361 caaatcaatc tttttatgtc aagggatttt tacaaaatag aggcaaaaac tgcacctttc
     6421 tgtgaaaaat ttcacaataa caaaaaactt aggaaaataa aatatttaaa taatcccgtt
     6481 tcccatacaa aattcgaata attcgaaccg ccggagtatc ttctttataa aacaccaaat
     6541 aatttccact cactataaaa cgatactgtg tttctatatc aataattgaa gacaagggcg
     6601 taccactact aggaaagtct tggaggtttc gaatagcttt tattattttt gatacagtat
     6661 tttgagcagc cgtcggactt tctagctctt cacttatata tttttttata tcatgtaagt
     6721 cttttttggc ttcgggggaa aataaaatag caggcattat tttataccta gatcttcttc
     6781 tatttcttca agttttatcc atcccccttg ctcagctacg acttctcctt ttgcaaggtc
     6841 tgcaaagagc tttaaagtag cttgtgtttt tcgatagtct tcaatatcca taagagcata
     6901 ctttcctcgc ccgttctttg ttaaaaaaac aggttctccg actgcgacat ctttcaatac
     6961 aatattataa ctgcgtaaat cagaaacagg ttttatattt gccaaaacat acctccacta
     7021 actaaaattg tagcagaatt ttacgtaaaa tacaacagca aaatnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnntttttc tttacgaaag cgcttttctc ttctataata
     7201 gggctatgtt ttattatagc tatgaaacgc ccattggtat cttgggcata gaggaaaacg
     7261 agggttttat tacccggctg gattttttga aagataaaaa cgaaggtccc gcacaaaaaa
     7321 cgatagctga aacgcctctt ataaaaaaag catacgagca attgaccgaa tatttccagg
     7381 gggaaagaag ggtctttgag cttcccttaa agccaatggg aactgatttt caaaaaaaag
     7441 tttgggaggc tcttatcgcg atagattatg gcaagacaaa aacctacaag gcaatagcag
     7501 aacgtgtcga aagtcccaag gcctttcgtg cggtaggagg tgcttgcaac aaaaatccca
     7561 ttgctatttt tatcccctgt catcgtgttg tcggagcaaa tggcagcctt gtcggttttg
     7621 ctggcggctt gtcaactaaa gattttttac tcaagcttga agatacacaa aaccatttat
     7681 tttaagagcc aaaattgatt gcctttcgct tttctcattt ttgcctcccc ttgctataga
     7741 agcctttttt ttgtaaaata tcttctatat ataatacaaa aggacaggat acatgataga
     7801 tacaaaacgc cccatatttc ccaaaaaagc tgtagttaca ggtggaatgc cctatggcaa
     7861 taagcgcctg catcttggac acatcggagc ggtttttatt cctgccgata tttacagccg
     7921 atttttacgc gatagaatag gaaaagaaaa tgttgtcttt gtttctggca ctgattgcta
     7981 cggctcgccc attgtagagt attataaaaa agctattgcc gatggcagtt tttctggcag
     8041 cctcgaagac tttgttttat caaaccacaa ggcgcaaaaa aacgagcttg atatgtactc
     8101 cattagcaat aatctttttg caacttccgc cctcggtaga tcgggtgaaa ttcatcggga
     8161 gctgagcgct gaagttttga aaaccttgca taaaaacggg cacttggaaa aacatgtgcg
     8221 accacaattt tacgatgcaa aactcaaagc ctttctcaac gggcgtcagg ttatcgggcg
     8281 ttgtcccata cctggatgca agagcgaaaa agcctatgcg gatgaatgtg agctcggaca
     8341 tccctatgag ccaaaagatt taattgcccc aaaaagtgcg ctctctggtg aagcacctga
     8401 aatgaaggat gttacaaact ggtatataaa ccttgcctct tttgccgatg aatttgacaa
     8461 atggataaca tggcttgaaa aagatcctgg cacacgaagt tttattaccg caacaattaa
     8521 agaattttta gcaaagccaa cgctctatgt acggcaagaa cacgaagatg aactcgaagc
     8581 tattcgtcct cttttacctc cacaccgcat taaggaagat ccaagcaagg ctattccctt
     8641 ggaatttaac tcgctctctg aatgcgacaa ggcgggagaa atcttgtttg aaaaaaacat
     8701 tcgatttcgt gcaggtaaaa cgcttacccc ttttcgcctc acgggcaata ttgaatggag
     8761 tatacaggca ccaactatag atggtgtgga aggtttaact ttttgggtat ggccagaatc
     8821 gctttgggct cccatctctt ttacaaaaac ctatcttgaa tcgatcggta aggatgcgga
     8881 cgaatggaaa aaatactggt gttcaaaaga tgcccttgtc tatcagttta ttggggaaga
     8941 taatatttac ttttacagtc ttgcacaaga ggctattttt atggcacttc agggagaaaa
     9001 acccgaagcc tttccagcag atggtttttt gcaagcaaca cagctcatcg caaacaaaca
     9061 tcttttacaa ggtaaaaaaa aggcttcgtc ttcaggggat gaaaaaccca tcatggcaga
     9121 agatttattg aagtactata caagcgacca attgcgagcc cactttttcg cactgggttt
     9181 agggcttcgt tctataagct ttaatcccaa gccactcaat ccagatgcaa atccgcgaga
     9241 gagcgatgcg gttctcaaag aaggtatgct tctaagcaat gttacaaacc gtcttgcgcg
     9301 atcttgtttt tataccagtc aaaaatactt tgacggaaag cttcccgaag gctccgtaag
     9361 tgatgcgata aaaaaagcct gcgacacagc catcctcgaa tacgagcggc ttatgtatac
     9421 gcatgagttc catgccatca tgatgctcct cgacaccttt attcgagatg caagtaagtt
     9481 ttgggcaagt gagtcaaaag aggcgacaca aaaagcagaa aaaaaagtgg gcgagcatgc
     9541 aagcatagaa gaaaaaatgc aagcagaggc agactatatg aaaagcgtcc tagttgacag
     9601 ctttcattac ctacgaacga ttatagccct acttcaccct atagctcccg tcggtagcca
     9661 aaaagtcttc gaatacctga aactcgatga gtctttttgg tcttgggata caatctttga
     9721 acccttgcat tttttcatcg atgaatcaca cgtgttcaag tttttagaac cacgcgttga
     9781 cttctttgaa aaacacgaaa ctcaggttgg aaaataaaaa gccaagcaga gaagctaagg
     9841 ctcgataaaa aaaagctacc aaaactgtgg tagctttttt tattcttt
//
LOCUS       DAOSSW010000068         9803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_6684_length_9803_cov_8.748872, whole genome shotgun sequence.
ACCESSION   DAOSSW010000068 DAOSSW010000000
VERSION     DAOSSW010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 9803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9803
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6684_length_9803_cov_8.748872"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..751)
                     /locus_tag="PKW26_06395"
     CDS             complement(50..751)
                     /locus_tag="PKW26_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93231.1"
                     /translation="MRTKRVAIFVLLLVSVGFLFGASYVNNEYNRLSKQYAVKAQEAF
                     DEGEYDLSIEYSYKSKDYAEMSETYIRVMLEKAEADKQIRLAKNQKLRAEQLQGQQNF
                     PMAFTAGETALKNALEAYGNEDYVSAASYALAAYASFGGIKEVQPLPKYYVVRPWAES
                     KDCYWNIAGRSYVYNNSLLWENLYQANKSSMRDPEDPDLIYPGMKLLIPSISGELREG
                     EFSTSKTYDPYTPAR"
     gene            complement(755..1648)
                     /locus_tag="PKW26_06400"
     CDS             complement(755..1648)
                     /locus_tag="PKW26_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93232.1"
                     /translation="MNKGFKVFTFLLCASLALMFFSCKSTPKVDDVEEVVEVVEEEAS
                     DKKALSDLESLHAKIEAARKEAIKNEADSLYPESFQFVDAEYEAHKMFTNTKIVASEH
                     IASAQRVLNRYEALNFSALATKAKKRVDGFQFKSYDEAAYALGVEKLSEAEKGYLADF
                     DTETQRTVAEEAYFAFRSILNKAFKDLATTKRQELIALRAEVDAIKAGAADKAGYNSA
                     MLVFTAGEANFAEGEYEDAYSEYLDAHDALSELYERVLEKRAAALRAMEEAREKSEAV
                     DKFALEADEIAPLENEDGNEE"
     gene            1801..2022
                     /locus_tag="PKW26_06405"
     CDS             1801..2022
                     /locus_tag="PKW26_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HOQ93233.1"
                     /translation="MADDFSFEIQKHVGILSTSKSGWTLELNLVSWGGRDAKYDIRSW
                     SPDHQKMGKGVTLSKEELTSLKAVLEAEV"
     gene            complement(2019..3017)
                     /gene="holA"
                     /locus_tag="PKW26_06410"
     CDS             complement(2019..3017)
                     /gene="holA"
                     /locus_tag="PKW26_06410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOQ93234.1"
                     /translation="MAASAFIYTGPEFGQKNEAIQQLKQNHLKAFGSVDEYSFYASET
                     KLNDVFSLLQNGSLFSAGRFALIKNAEVIKKKEDIALVAEWVAIAKKDPSSCVVFVSD
                     ENAVDKKLSNLVPKENQKIFWELFENQKEAFVRNFFKSMGLDIETDAIEAVLDMVENN
                     TEAIKRNCERFAYLFPKGSSISQENIEDVLVHTREESAFTLFDALAEANQNETERLDS
                     ALTILQNILQSKSRAGIQIIAGLTYSFRKLRTWQELHKNTRPSDFDLKTKGFSSKRAQ
                     TQYRKAGSLWTAEDVVLILALLAKTDMELRSANAALEETLMTSLVYGIHTKRDFYA"
     gene            complement(3017..3667)
                     /gene="lexA"
                     /locus_tag="PKW26_06415"
     CDS             complement(3017..3667)
                     /gene="lexA"
                     /locus_tag="PKW26_06415"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOQ93235.1"
                     /translation="MKKLTERQEEFFAFICSCIEDHSCPPTVRETAEYFGVSPRAVQD
                     QFTALENKGYVRRIENRSRSLKLLVSKEEYFMGSVEDEESEEEKARKEGISIPILGTV
                     AAGLPLFCEENYSGSLTLYPPIINKTDAFFALNVRGDSMINAGILDGDLAIVKQQSTG
                     RNGQIVVALLDDTVTLKRFYKEPARVLLKPENPKYNPIYCQDVKILGTLVTIIRTY"
     gene            complement(3664..3930)
                     /locus_tag="PKW26_06420"
     CDS             complement(3664..3930)
                     /locus_tag="PKW26_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOQ93236.1"
                     /translation="MKEKLLTVRNRAGIHARPAALIAQTANKFASEILIERGDTVINA
                     KSIMGVITMAAGYNTTLTLRATGSDEEAAVAAIYALFESKFEEE"
     gene            complement(3943..4932)
                     /gene="hprK"
                     /locus_tag="PKW26_06425"
     CDS             complement(3943..4932)
                     /gene="hprK"
                     /locus_tag="PKW26_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOQ93237.1"
                     /translation="MAKKEFSVLNLLDLELEGHNSLHLRCLSGRRGLVRTIQVPDLNR
                     PGLALSGFFDSFAHERVQVFGLGEVSYLRKLEKEKKDVDLRTFFSQEIPCCVFTHDLE
                     PTEQFAKISEEVGCPLLQTSLKSTDFSSRILRVFSNIFAPSQTLHGVLVEVYGVGIIL
                     LGDSGVGKSETALELIERGHRLVADDTVEIRCVNGNTILGQGANKIISHHMEIRGLGI
                     INIAQLYGVGAIREQKKVQLVVKLAEWDADKVYDRLGTKQNTTDLLGVKVRLIEIPVR
                     PGRNVPIIIETAAMNERLQSMGYHSARDFNINVLKWIETGDAQAAYYGSEDVY"
     gene            complement(4978..5268)
                     /locus_tag="PKW26_06430"
     CDS             complement(4978..5268)
                     /locus_tag="PKW26_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOQ93238.1"
                     /translation="MTITTNAVGFVLDEDQQALIDKKMERIKYADDLIVDLLLRIKYD
                     KKMIFDVTVNFRWGTVAHISSDDYDFTAGLNKMMDVLDSKIKKEKDKIQQKG"
     gene            complement(5464..6846)
                     /gene="rpoN"
                     /locus_tag="PKW26_06435"
     CDS             complement(5464..6846)
                     /gene="rpoN"
                     /locus_tag="PKW26_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOQ93239.1"
                     /translation="MKQGFQLQTRQQLSLSMTPQMIQSISMMALSTEELIDRIYEEVE
                     KNPALEIIKESSYKDVQPRQKKEGSPSSQSDEYQAFLESLPSKQKSLKEHLLSQLSFH
                     KLSDEEKESAKRIINNLSDEGFHLVDFDELFAGAIPKHLEKILALIQHFEPVGLATSG
                     VQESLLVQALSQADSTPLVIALLKLEPDVFTRVFTPFRPTLITKKLNALGEPLFQNLE
                     VKELEEALDFIKNLDPHPARDFSLSQASYVIPDIKIRRATADEKDESGEDFVIELLRG
                     VLPELAISKSFENFRLDKTKEKSKKDEERFVKEAVQDASWFLNVLAQRTITIVKVARL
                     ILQIQKEFFQKGSPAYLKPLRMKDLADEIEVSESTISRIANNKYLACDWGILPFKFFF
                     TTEVATSRGGSESRESVKEKIREILADDNANISDQKLADKLALQGIKIARRTVAKYRS
                     ELDIASSFDR"
     gene            6893..8209
                     /locus_tag="PKW26_06440"
     CDS             6893..8209
                     /locus_tag="PKW26_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOQ93240.1"
                     /translation="MSKKKNLQKYGIAIPSILLPKDKSKLKSWSVIACDQYTQDAAYW
                     QNVENFVGDAPSSLHITLPEIYLNASDKNERIASIKSTMKKYIDEKLFEDEGDAFVYI
                     ERKTAYGRKRSGLVLAVDLECYEWKPFSQSLIRATEATILDRIPPRKEIRQNAAIETP
                     HIMLLVDDPEKKLVEKLGEKTQTEAPLYQTDLMMDSGSITAWKIHTEKQFTYIEETLA
                     ELAQQNKQKDGSVFLFAVGDGNHSLATAKAVWDSYKTEQNLSDHPARYALVEIVNIYD
                     AGLTFEPIHRVIFDIDGKELLAFCQKELGGEILFCENEAELTEAVENSSSNFGFVYNH
                     EGKICYALLKNKSETLAVSRLQPVLDSYLEIHAEKNPEIDYIHGNDEVFRLGGKNTAT
                     SILLPPIAKDSFFSSISNNGPLPRKSFSMGEASEKRFYMECRKLFS"
     gene            8314..8396
                     /locus_tag="PKW26_06445"
     tRNA            8314..8396
                     /locus_tag="PKW26_06445"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8347..8349,aa:Leu,seq:caa)
     gene            complement(8406..>9803)
                     /locus_tag="PKW26_06450"
     CDS             complement(8406..>9803)
                     /locus_tag="PKW26_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOQ93241.1"
                     /translation="ERVLISLSPADLKKEGAGFDLAIALAVLAESNLLGSSWSFEPNV
                     LVMGELELSGAVRKVRGVHAAVSTAYEQGFRYCIVPKENEVEASFKSGMRVIAVEHLI
                     EAFNEMSALSLLVKNKSETDAQYKDALGLHEDLSNLKIDFFPVRDEEDFVYVRGLDFL
                     VRGLQIAAAGGHNLMAYGPPGCGKTLALQRFPGLLPKLTHHEALPVTRIYSLAGLLKA
                     EENLIEKRPFRMPHQTASVEGIIGGGPYCHPGEVSLAHNGVLFLDEAAEFRANVLQAL
                     RVPLEEGRVSLSRAGRSTSFPARFQLLIAANPCPCGNFGVKNKICLCAPRAVESYWKK
                     FSAPLLDRVDIRIPVFNKIQNIMDSSIQGMSTKDLRKPIQSAFKMQFTRQGKLNASLK
                     AEEIEDFCHIEKALKEKLLVKSSEQDFSSRAIHSVLKLSRTIADMQERETIIEADLDE
                     AIRYRKNEGGLDILF"
BASE COUNT         2827 a         2060 c         1930 g         2986 t
ORIGIN      
        1 tgatgggcac agccttgcat ttatttagac aacactgtga ctaaaagatt tagcgcgctg
       61 gtgtataggg gtcataggtc ttgctggtgc taaattcacc ttcacgcaac tccccagaaa
      121 tgcttggtat aagcaatttc atgccggggt aaatcaaatc ggggtcctcg ggatcgcgca
      181 tagaagattt atttgcctga tagaggtttt cccacaacaa agaattattg tagacataac
      241 ttctaccagc aatgttccag tagcaatctt ttgattcagc ccaaggtctt accacataat
      301 attttggtaa aggctgtact tctttaatgc cgccaaaact tgcataggct gctaaggcat
      361 agcttgccgc agaaacatag tcttcatttc cataagcttc taaagcattt ttcaaagcag
      421 tttctcccgc agtaaatgcc ataggaaagt tttgctgtcc ttgcagttgt tctgctcgaa
      481 gtttttgatt ttttgcaagt cgtatttgct tatcagcctc agctttttca agcataactc
      541 gtatataggt ttcggacatt tcagcatagt cttttgactt atacgagtac tcaatcgata
      601 aatcatactc accttcatcg aacgcttctt gcgcctttac agcatattgt tttgaaagcc
      661 tattgtattc gttatttaca taacttgcgc caaataaaaa gcccacagaa actaataata
      721 aaacgaaaat agcaacacgt tttgttctca ttatctactc ctcatttcca tcttcatttt
      781 ctaatggtgc aatctcatcg gcttctaaag caaacttatc aacagcttcg cttttttccc
      841 ttgcttcttc catagcacgt aaagcagcag cacgtttttc aagcaccctt tcgtataatt
      901 ctgacaatgc gtcatgggca tcaagatatt cgctataggc atcttcgtac tcgccctcgg
      961 caaagtttgc ctcccctgca gtaaatacta acatagcaga gttatatcca gccttatctg
     1021 ctgctccagc cttaattgcg tccacttctg cacgtaaagc tatgagctct tgacgttttg
     1081 ttgtagctaa atctttgaac gccttattta atatagacct aaaagcaaaa tacgcttctt
     1141 ctgctacagt tctctgtgtt tccgtatcaa aatctgccaa atagcctttt tctgcttcgg
     1201 aaagtttttc tacgcctaag gcataggctg cctcgtcgta agacttaaac tgaaagccat
     1261 caactctttt ttttgccttt gtagccaaag ctgaaaagtt aagcgcttca taacgattta
     1321 aaactctttg tgcagaagca atgtgttctg atgcaactat tttagtgttt gtaaacatct
     1381 tgtgagcttc gtattcagca tcaacaaact gaaaagactc aggataaaga gaatctgcct
     1441 catttttaat cgcttctttt ctcgcagcct cgatttttgc atgcaagctt tctaaatcag
     1501 aaagtgcttt tttatcactt gcttcttctt cgacaacttc gactacctct tcaacatcgt
     1561 ccacctttgg tgtagattta caagaaaaaa acattaaagc taggcttgcg cacaacaaaa
     1621 aggtaaaaac cttgaaccct ttattcattt aaacctccat ttttttacac attaacgtat
     1681 aaagattaca ctacttttaa aacaaacaca agtttttttt tctttttgtg ttttaaaatc
     1741 aaagaaacta tgttattatg tacactatac atacaaaatt ataaaaatac ggaggaaaaa
     1801 atggctgatg actttagttt tgaaattcaa aaacatgttg ggattttaag cacttcaaaa
     1861 tcgggatgga cgctcgaact aaatctcgta tcctggggtg gaagagatgc gaagtatgat
     1921 attcgtagtt ggtcacctga tcaccaaaag atgggaaagg gcgtaacgct cagtaaagaa
     1981 gagcttacaa gcttaaaagc ggttttagaa gcagaggttt aggcgtaaaa atctcgtttt
     2041 gtatgaattc catacacaag gctagtcata agcgtttctt ctaaggctgc atttgcagaa
     2101 cgaagctcca tatcggtttt tgcaagaagg gcaaggataa gcacaacatc ttcggctgtc
     2161 cacaagctcc ctgcctttct atactgcgtt tgtgctcgtt tactggaaaa gccctttgtt
     2221 tttaggtcaa aatccgatgg tcgcgtattt ttatgcagtt cctgccaagt cctaagttta
     2281 cgaaaactat aagtaagccc cgcaattatt tgaatacctg cacgagattt tgactggaga
     2341 atattctgta aaatagtcaa ggctgaatct aagcgctcgg tttcattttg gtttgcttcg
     2401 gctaaggcat caaaaagagt aaacgcagac tcttcccttg tatgtaccaa gacatcttcg
     2461 atattttcct gacttatact actgcccttg gggaataaat aggcaaaacg ctcacagttt
     2521 cgcttaatcg cctcggtatt attttccacc atgtcgagaa ccgcttctat cgcatctgtt
     2581 tcaatatcta agcccataga cttaaaaaaa ttacgcacaa aggcttcttt ttgattttca
     2641 aacaattccc aaaatatttt ttgattttct tttgggacaa ggtttgaaag ttttttatcg
     2701 acagcgtttt catcggaaac aaaaacaaca cagcttgacg gatctttttt tgcaattgca
     2761 acccactccg cgactagggc aatatcttct tttttcttta taacttccgc attttttata
     2821 agcgcaaatc gaccagctga aaaaagcgag ccgttttgta aaagcgaaaa aacatcgttg
     2881 agtttcgttt ctgaggcata gaaagaatac tcgtcaacag agccaaaagc cttaagatga
     2941 ttttgtttta attgctgtat tgcctcgttt ttttgcccaa actctggacc ggtataaata
     3001 aatgcggaag ctgccatcaa taggtcctta taatcgtaac aagggtgccg agtattttta
     3061 catcttggca ataaataggg ttgtattttg gattttcggg cttgagaagt actcgcgcag
     3121 gttccttata aaagcgcttc agtgttacgg tatcgtccaa taaagcaacg acgatttgcc
     3181 cgtttcgtcc tgtagattgc tgttttacaa tggcaaggtc gccatcgaga atgcctgcgt
     3241 ttatcatgct gtcaccacgg acattgaggg caaaaaaagc atctgtttta ttgattatag
     3301 gcggatagag ggtaaggctt ccagaataat tttcttcgca aaaaagcgga agtcctgcag
     3361 caacggttcc aaggatggga atggatatgc cttcttttcg agctttttct tcttctgact
     3421 cctcatcttc gacggagccc ataaagtact cttcttttga tacgagcagt ttcaaagaac
     3481 gagacctgtt ttctattcga cgtacataac ctttattttc gagagcggta aactggtcct
     3541 gcacagcacg aggagagacg ccaaagtatt ctgctgtttc tcgaacggta ggaggacatg
     3601 agtgatcttc aatacatgag caaataaaag caaaaaactc ttcttgccgc tctgttaatt
     3661 ttttcattct tcttcaaatt tggactcgaa caaggcgtaa atagcggcga cagcggcttc
     3721 ttcatcgctt cctgttgcac gaagggtgag cgttgtgtta tagccagcag ccatggttat
     3781 aacgcccatg atggattttg cgtttattac cgtatcgcct cgttctataa gtatttctga
     3841 ggcaaattta tttgcagttt gtgcaatcaa tgccgcaggt ctcgcatgaa ttccagctct
     3901 attacgaact gtaagcagtt tttctttcac tagttactcc aatcaatata cgtcttcgct
     3961 accataatag gcggcttgtg catctccagt ttcaatccat tttaacacat ttatgttaaa
     4021 atcacgagct gaatggtagc ccattgattg aagccgttca ttcatagctg ctgtttctat
     4081 aataataggg acgtttcgcc ctggacggac ggggatttcg atgaggcgga cttttacccc
     4141 gagtaaatcg gtggtatttt gttttgtgcc aagcctatcg tagaccttgt cggcgtccca
     4201 ttcagcgagt tttaccacaa gctgtacttt tttttgctcc cgtattgcac caacgccata
     4261 gagttgtgca atattgataa taccgagtcc acgaatttcc atgtggtggc ttataatctt
     4321 gtttgcaccc tgccctagaa tagtgttgcc gtttacacag cgtatttcaa cggtatcgtc
     4381 ggcgacaagg cggtggcctc tttcgatgag ttcaagggcg gtttcgcttt ttcctacacc
     4441 ggaatctcct aaaagaatga tgccaacgcc gtagacttcg actaaaacac cgtgaagtgt
     4501 ttgactggga gcaaaaatat ttgaaaaaac ccgtaaaatc ctactcgaaa aatcggtcga
     4561 tttgaggctt gtttgcaaaa gcgggcaacc cacttcttca gaaattttgg caaactgttc
     4621 cgttggttct aggtcgtggg taaagacgca acaaggtatc tcttgggaaa aaaaagttcg
     4681 caagtctacg tcttttttct ctttttcgag ttttcttaga taggaaacct ctcctaagcc
     4741 aaaaacttgg acacgctcgt gggcaaagga gtcaaaaaag cccgaaagtg caagcccggg
     4801 acgatttaag tcggggacct gtatagttcg cacaagccct ctgcgcccac tgagacagcg
     4861 tagatgaaga gaattatgtc cttcgagttc taaatcgagt aaattgagaa ctgaaaattc
     4921 tttttttgcc atattcgaac tatacactca accgaggctt tatacaagtt gtattagtta
     4981 tcctttttgt tgaattttat ccttttcctt ttttattttt gagtctaaga catccatcat
     5041 tttgtttaaa cccgcggtaa agtcataatc atctgaagaa atatgtgcga cagttcccca
     5101 tcgaaaattt accgttacgt caaaaatcat ttttttatca tattttattc ttaaaagcaa
     5161 atcgactatc aaatcatctg cgtatttaat gcgttccatt tttttatcga ttaaggcttg
     5221 ttgatcctca tccaatacaa agccaactgc atttgttgta attgtcatag tgcctcctaa
     5281 tagcgccata gggctttttt tattcttatt ttctaagaat tgtatatctc aattataaca
     5341 gtttttaaga taaaaagcaa aaaaaatcta ggagataata aaagaaatct caagaaaatt
     5401 gattttatcg tattgattta tgtaagggag gtgaatattt tttgaaaagg ctatctatct
     5461 gctctatcta tcaaaagaag aggcgatatc tagctcgctg cgatattttg caacagttcg
     5521 cctagcaatt tttatgcctt ggagggcaag tttgtcggca agtttttggt ctgaaatatt
     5581 tgcattgtcg tcagcaagga tttctcggat tttttccttg acgctttctc gagattctga
     5641 gcccccccta cttgttgcaa cttcggtagt gaaaaaaaac ttaaagggca agatgcccca
     5701 atcgcaggca aggtatttat tgtttgcaat gcgagaaata gttgactcgc tcacttcgat
     5761 ctcatctgca aggtctttca tgcgcaaggg ttttaagtag gctggggaac ctttttgaaa
     5821 aaattctttt tgtatttgta agataaggcg ggcaactttt acaatcgtaa tcgttctttg
     5881 tgccaaaaca tttagaaacc aggatgcatc ttgtaccgct tcttttacaa aacgctcttc
     5941 gtcttttttc gatttttctt ttgttttatc aaggcgaaag ttttcaaaac tctttgaaat
     6001 agcaagttca ggaagcacgc cgcgtaaaag ctctatgaca aaatcctcgc cactttcatc
     6061 tttttcatcc gctgttgccc tgcgaatttt aatatctggg ataacatagc ttgcttgact
     6121 gagagaaaaa tctcgagctg gatgtgggtc aaggtttttt ataaaatcaa gcgcttcttc
     6181 aagctccttt acttcaagat tttgaaaaag cggttcacct aaagcattga gttttttggt
     6241 aattaaagtc ggtcgaaatg gcgtaaaaac acgagtgaaa acatctggct caagcttgag
     6301 caaggcaatg acaaggggtg tcgagtcggc ttgggagagg gcttgcacga gaaggctttc
     6361 ttgcactcca gaagttgcaa gtcccactgg ttcaaagtgc tgtatgaggg caagaatttt
     6421 ttccaaatgt ttgggaatag cgcctgcaaa aagctcgtca aaatctacta aatggaagcc
     6481 ctcatcgcta agattattga taatgcgctt tgcactttcc ttttcttcat cacttaattt
     6541 gtgaaaagaa agttgggata aaagatgttc tttgagagat ttttgctttg acggaaggct
     6601 ttctaaaaaa gcttggtatt catctgactg tgatgacggg ctcccttctt ttttttgtcg
     6661 cggttgcaca tccttatagc tactttcttt tattatttcc aaggcaggat ttttttcaac
     6721 ttcttcataa atcctatcga tgagttcttc cgtcgacaaa gccatcatcg atatggactg
     6781 tatcatctgc ggtgtcatgc tgagagaaag ttgttgtctt gtctgtagtt gaaagccttg
     6841 cttcatactg catagtatag catatttttt tttgtttcga tataatacgc acatgagtaa
     6901 aaagaaaaac cttcaaaaat atgggatagc tatcccgagc attttattgc caaaagataa
     6961 atcaaagtta aaaagttggt cggttattgc ctgtgaccaa tacacacaag atgccgcata
     7021 ctggcaaaat gttgaaaact tcgttggaga tgctccttca agcctacata taacgcttcc
     7081 tgaaatatat ctaaatgcaa gcgacaaaaa cgaacgcatt gcttcgatta aaagtactat
     7141 gaaaaaatac atagacgaaa aactttttga agatgaggga gatgcctttg tctatatcga
     7201 aagaaaaacc gcctatggaa gaaaacgaag cggccttgtt ctggcagttg acctagaatg
     7261 ctacgaatgg aaaccttttt cgcaatcgct tattcgagca accgaggcga caatccttga
     7321 ccgcattccg ccccgaaagg aaatacggca aaatgcggca atagaaacgc cgcatattat
     7381 gctcctcgtc gatgaccctg agaaaaaact tgtagaaaaa ctcggcgaaa aaacacagac
     7441 cgaggctccg ctatatcaaa cagatttgat gatggactcc ggttctatca ccgcatggaa
     7501 gatacatact gaaaaacaat tcacatatat agaagaaacc ttggcagaac ttgcgcaaca
     7561 aaataaacaa aaagatggct ctgtgttttt gtttgctgtg ggggatggaa atcatagcct
     7621 tgcaacagca aaggcggttt gggattcata taaaaccgag caaaatctaa gcgaccatcc
     7681 agcgcgctat gccctcgttg aaatagtcaa tatctacgat gcaggtttaa cctttgagcc
     7741 aatccatcgt gtcatatttg atatagacgg aaaagaattg ctcgcgtttt gccaaaaaga
     7801 acttggtggc gaaattcttt tttgcgaaaa tgaagcagaa ctcacagaag ctgtggaaaa
     7861 ctcaagctca aactttggct ttgtctataa tcatgagggg aaaatatgct acgccctcct
     7921 gaaaaataaa agcgaaaccc ttgcggtgag tcgcttacaa cctgttttag atagctacct
     7981 tgaaatacat gcagaaaaaa atcctgaaat agattatatc cacggaaatg atgaggtctt
     8041 tagactcggc ggaaaaaata cggcgacaag tattttattg cccccgattg caaaggatag
     8101 ttttttctct tctatatcca ataatggtcc gcttccgaga aaaagttttt ctatgggaga
     8161 ggcaagcgaa aaacgctttt acatggaatg cagaaaactt tttagctaaa aaaatagaaa
     8221 ataaaagctc gtattcttta ctttttttta tgactatggt attgtcatta gtagacagta
     8281 tcgatataaa aaaatcgatt cgaaaacaca aaagccggcg tggtggaatg gtagacacga
     8341 cagactcaaa atctgttgct cgcaagggtg taagagttca agtctctttg ccggcatttt
     8401 tcatatcaaa acaaaatatc caaaccgcct tcgttttttc tatatcgaat agcctcatct
     8461 aaatctgctt caattattgt ttccctctct tgcatgtctg caatggtgcg agaaagtttt
     8521 agtacgctgt gaatcgcccg agaagaaaaa tcctgctcgc tgctttttac taaaagcttt
     8581 tccttcaatg ctttttctat atggcaaaag tcttcgattt cttcagcctt aagacttgca
     8641 tttaattttc cttgtcgcgt aaattgcatt ttgaaagcgc tttgaatagg ctttcgtaaa
     8701 tcctttgtcg acatgccctg aatagagctg tccatgatgt tttggatttt attgaaaact
     8761 gggatacgga tatcaactcg gtctaagagc ggggcagaaa attttttcca gtaggattca
     8821 accgcgcgcg gcgcacagag gcaaattttg tttttcacgc caaagttgcc acagggacag
     8881 gggtttgcgg cgattaaaag ctgaaaccgc gcaggaaaac tcgtactacg ccctgcacga
     8941 ctcaaactca cccgtccttc ttctaggggg acgcgtaggg cttgcaaaac atttgctcga
     9001 aactccgccg cctcatcgag aaataaaaca ccgttatggg caagagaaac ttctcccgga
     9061 tggcaatagg gaccaccgcc gataattcct tcgacgcttg ctgtttgatg cggcatgcga
     9121 aagggacgct tttctatgag attttcctca gccttgagta agccagcaag actataaatg
     9181 cgcgttacgg gaagcgcctc gtggtgagtg agcttgggca aaagacctgg gaaacgttgc
     9241 agggcaagcg tttttccaca accgggcggc ccgtaagcca tgagattatg tccccctgct
     9301 gcagcaattt gcaaacctcg aacaagaaaa tctaaaccac gtacatagac aaaatcttct
     9361 tcgtcgcgca ctgggaaaaa atcgattttt agattgctta aatcttcatg gagacctaag
     9421 gcatctttgt actgcgcatc ggtctcgctt ttatttttta ccaaaaggga gagcgctgac
     9481 atttcattaa aggcttctat gagatgttcg accgcaatca ctcgcatacc gcttttaaaa
     9541 gaagcttcta cctcattttc tttgggaaca atacaatagc gaaacccctg ctcataggct
     9601 gtcgataccg cagcgtgaac accgcgcacc tttcgaaccg ctcccgaaag ctctaactca
     9661 cccataacca aaacatttgg ctcaaaggac caactggaac ccaaaagatt tgattcagca
     9721 agaaccgcca gtgcaatcgc taagtcaaaa cctgcacctt ctttttttaa gtccgctgga
     9781 gataaactaa taaggacacg ctc
//
LOCUS       DAOSSW010000069         9659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_6816_length_9659_cov_6.421387, whole genome shotgun sequence.
ACCESSION   DAOSSW010000069 DAOSSW010000000
VERSION     DAOSSW010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 9659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9659
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6816_length_9659_cov_6.421387"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..799)
                     /locus_tag="PKW26_06455"
     CDS             complement(26..799)
                     /locus_tag="PKW26_06455"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOQ93242.1"
                     /translation="MEKIFTHLGVRESLFSHADPQYKQFNKKLIPHIDEREMIGVRTP
                     VLRMLAKNIANTNWRDFLQESWHFAHAEGSGALYFEEKMLHGFILGAVKNTELDELFH
                     YIKYHIRYLDSWAAVDSFASSLKIAKKSPLHENYLKGFLEPYFYLPNIEQNEYQVRFA
                     LVILHTSFIKKDNLDYIFDRVSKVKHSGYYVKMAKAWLLSICFVHFPNETKEVLFDKK
                     NPLALDEWTLKKTMQKIRESKRCTTEDRAFLNEKAKNLF"
     gene            complement(969..1643)
                     /locus_tag="PKW26_06460"
     CDS             complement(969..1643)
                     /locus_tag="PKW26_06460"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOQ93243.1"
                     /translation="MEQETYAMPLIGEAAPSFKAVTTQGEINFPEDYKGKWVILFSHP
                     ADFTPVCTTEFMTFASMAEEFRSLNTELVGLSVDSLYAHIAWLRKIQELEWNGMKNVE
                     VHFPLIEDIKMDVAKKYGMIQPGQSNTQAVRAVFVVDPQGKIRTILYYPLSMGRNFDE
                     IKRIILALQKADADGVATPADWRPGDDVIIPTPGSCGTAKERVESKDPDQYCLDWFMC
                     FKREKK"
     gene            1896..3560
                     /gene="sulP"
                     /locus_tag="PKW26_06465"
     CDS             1896..3560
                     /gene="sulP"
                     /locus_tag="PKW26_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HOQ93244.1"
                     /translation="MFKPAIIQSLKNYNTKTFLSDLIAGLIVGIVSLPLSIAFAIASG
                     ASPERGIITSIIAGIIIAIFGGSKVQIAGPTGAFVIIIYGIIENFGMSGLLVTTILAG
                     LILIAFGIFKFGGLIKFIPHTIVTGFNAGIALSIFTSQIGDFLGLQTGKMPIQFISKI
                     SIYLKTISTINIHALLLGTLSLAIIFLWPRITKKIPGSIIVIIVATAISQIFKLPIET
                     IGSRYGSIPSTIPMPKIPNFSLDLLVSLFPQAISLAVLVSIESLMTAVVADGMTGKKH
                     NSNTELIGQGIANIICPLFGGLPSIGALARTATNIKNGGQTPIASIVHSIVLLVIMLF
                     LGQFVIFIPMAALAAILISVAINMAGIKDIRFLLRGQKSDISVLVVTFLATVFIDLTV
                     AIAIGLVLAAFLFIRKIIEVSAVYTTNVQSQRIFQGLDDEFKTDIEEKELSIPKGVLI
                     YEIEGPLFFGTVQKFENAVARAEVDFKVLIVRTRRMIYLDAEGLHALNQVQELCKRRN
                     VQLLISGIHTQPYTLLVKSGFEKKLGENAIFQKIDDALAKAEELVQ"
     gene            complement(3586..>4020)
                     /locus_tag="PKW26_06470"
     CDS             complement(3586..>4020)
                     /locus_tag="PKW26_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ93245.1"
                     /translation="LEYRLLGSISVENVLLSGFYDSIGLYDAPKESEKKAVLFWLALA
                     NMSECKDKNFANLSYGMQRAILILRSVIKTPRLLILDEPCHALSPSERSFVLALIEEI
                     AKQSATTILHVSHDETEFLDCEKKIFRFLPETEKGYRIEYRS"
     assembly_gap    4021..4120
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4121..4737)
                     /locus_tag="PKW26_06475"
     CDS             complement(<4121..4737)
                     /locus_tag="PKW26_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93246.1"
                     /translation="MKMFNPKKIEKNTGLFKVSFEEAHLLIERERKNDDSEFMEGGFD
                     PGTNLEIFLLQECFEKESKLHELIEKAHLQELWNVLSLEKLLNRGLKFLSTGEMKKVL
                     LFKAFLLLACRKIEKIILIDLYDGLDFETVAKLKDLLETEGSYLRMRFSNAIEMQKTN
                     EAFSFDKETSPKQEIDIRSALSQEIDSNQRGREKKELVRMEKVNVT"
     gene            complement(4724..6505)
                     /locus_tag="PKW26_06480"
     CDS             complement(4724..6505)
                     /locus_tag="PKW26_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ93247.1"
                     /translation="MRIFSYMKGSAWLLAIVFFCIILSSSAMIILVYMLKPLINSVLL
                     PLIGQQNPDLSHFARILFVVAIGAVFAALASYVNSRIMLHLSTKTLYKIRVDLFAKME
                     TLPIGFYDGKTHGDLMSLYTNDIDTLRNMLSQTLPQLFSSMLTIVSTFIMMLVLSPIL
                     TGLIVLMIGIMIFTVAKIGSKSGKAFRQQQAELGKVNGFIEELIEGQKLVKVFSREEK
                     VKEEFAVFNENLRKSGTRAQAYASILMPMMNSLSYLNYALTAIAGASFVILGHLDLGS
                     VAAFLQYSRNFANPITQMSQQFNSVLNALAGSERIFAVIDEDAEIDEGRVTIVKSEEN
                     KGEYLWKIPESDGSWRFEKVKGEIIFKDLNFGYREDKTILHDLSLHARPGEKIALVGS
                     TGSGKTTIINLINRFYEAPEGSILYDGIAITNIRKADLRRSISVVLQDVALFSGSIAD
                     NIRYGRLSASDAEVIDSAKKANADFFIQHLENGYETELSENGANLSQGQRQLLSIARA
                     AIKSPPVLILDEATSSVDTRTEKQIEKAMDELMKGKTVFVIAHRLSTVKNADHILVLE
                     KGRIIEQGTHESLLAQKGRYYSLYENV"
     gene            complement(6667..8439)
                     /locus_tag="PKW26_06485"
     CDS             complement(6667..8439)
                     /locus_tag="PKW26_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ93248.1"
                     /translation="MKSFKKLIGGLEKRFVLTSILAPLAIIVEVVLEVLIPLLMAKII
                     DQGIASQNLGLVARLGIIMVSLALLALVFGSLSGIFASFASLGFAKNLRKKLFSKVQD
                     FSFANIDSFSTASLVTRLTTDVTNVQQLYQMALRMFVRAPVMFVSATIMSFYINPSLA
                     RVFFVAIPFIFIPMIFILRKAFPRFSLMMKKYDALNARVQENVSAIRVVKTFVREDHE
                     NKKFREAADQVRSTQVSAEKLIILGMPLMQLVMYLSIIAIFWFGGNMIVNESLLTGEL
                     ISFISYLSQVLFSLMMISMIFVMLVISRASVQRIVEVLDAEVDLKDCIEVENEKTKTL
                     HIKDGSIQFSEVFFSYSNKKDKCILKDINIEIDSGMTVGIIGSTGSSKTSLVQLIPRL
                     YDVLSGAVKVGGIDVRDIPLKELRGQVAMVLQKNTLFSGTIKENLKWGNENASDAEIK
                     KVCQIAQADDFVSNFPEGYETKLGQGGVNLSGGQKQRLSIARALLKKPKILILDDSTS
                     AVDTATDKAIRSALKEGFADITKIIIAQRIDSIKESDLILVMDEGKLSAQGRHEDLLE
                     KSAIYREVYESQQAGALIDLDEEE"
     assembly_gap    8463..8562
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8563..8989)
                     /locus_tag="PKW26_06490"
     CDS             complement(<8563..8989)
                     /locus_tag="PKW26_06490"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOQ93249.1"
                     /translation="MKTKKILLTTILLCFVLVSYSFAQTAKVKSVTGKVEYSTDGTSW
                     SAVKENMSLAKGTIISTSFKSTAVIVINDSLLHVKPLTRMSLDELRKTQDEVQTELNL
                     VSGKVQVEVKPRAKQEVVNFTVKSAVATASVRGTGFVFDG"
     gene            complement(9023..>9659)
                     /locus_tag="PKW26_06495"
     CDS             complement(9023..>9659)
                     /locus_tag="PKW26_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93250.1"
                     /translation="LAFRQNVSLEFTGQNDLNSSGEDSVNSYYLSLGLNGPIIPSLDY
                     DLVSVYGIANVYNTIESYVLVDANLRLAIPTIKGQLSLEGLFPFALHDSGGSFKAITE
                     KPLSFVETAITATSLQSLTLTANMSLSSTVLLSAKFHNYFSQSPNAFSFVDPTKEGRY
                     VGSELVFNCSLIPTSDFSLGLTGGIMLFNKDLVEDTSLNPFKVSLTASLFI"
BASE COUNT         2826 a         1955 c         1653 g         3025 t
ORIGIN      
        1 tgatagagac aaaaaaaact aaagactaaa aaagattttt tgccttttca tttaaaaatg
       61 ccctatcttc agtagtacaa cgttttgact cccgaatctt ttgcatcgtt tttttcaatg
      121 tccattcatc caaggctaag ggattttttt tgtcaaataa aacttccttt gtttcatttg
      181 gaaagtgcac aaaacagata gaaagtagcc aagcctttgc catttttacg tagtagccgc
      241 tgtgctttac cttggaaacc ctatcaaaga tataatcgag gttgtctttt tttataaaag
      301 atgtgtgaag tattacgagg gcaaaacgca cttgatattc attttgctct atatttggta
      361 gataaaagta gggttccaaa aaacctttaa gatagttttc atgcagagga gatttttttg
      421 caatcttcaa gctcgaggca aagctatcga ctgcagccca tgagtccaaa taacggatat
      481 ggtacttgat ataatgaaaa agctcatcca attctgtgtt ttttacagcg cctaaaataa
      541 agccgtgaag cattttttct tcaaaataaa gcgcaccaga gccttctgcg tgagcaaaat
      601 gccaagattc ttgcaaaaaa tcacgccaat ttgtatttgc tatgttcttt gcaagcattc
      661 gtaacaccgg agttcgcacc ccaatcattt ctcgctcatc tatatgagga attagcttct
      721 tattaaattg tttatattga ggatccgcgt gagaaaacaa gctttctcta accccaagat
      781 gggtaaaaat tttttccata gtctgattat agcatgctat gaagcgagta ggtagagatg
      841 gaataaaaaa aagctaaccg tcaaaactgt tttcacagat ttttgggtta gcttttccat
      901 ggttctatgc gaagtgctgt attaccgcat cattcgataa gaccactcac tacatcttcg
      961 aaaaaagact atttcttttc tcttttgaag cacataaacc agtctaagca atattgatct
     1021 ggatctttgc tttcaactcg ctcttttgcg gtgccacatg aaccaggtgt tggaataata
     1081 acatcatcgc caggtctcca gtcagctgga gttgcaacgc cgtcagcatc ggctttttga
     1141 agtgcaagaa taattctctt aatttcgtca aagtttcgtc ccatactgag tggatagtac
     1201 aatatcgttc gaatcttccc ttgagggtcg acgacaaaaa cagcacgaac tgcttgcgta
     1261 tttgattgtc ctggctggat catgccgtat tttttggcaa catccatctt aatgtcttca
     1321 ataagtggaa aatgtacttc gacatttttc attccattcc attcaagttc ttgaatcttt
     1381 ctcagccatg caatgtgtgc atacaaggaa tccaccgata gtcctacaag ttctgtgtta
     1441 agggagcgga actcctcagc catagacgca aatgtcataa actcggttgt acaaaccggc
     1501 gtaaaatctg ctgggtgtga aaaaaggatt acccattttc ccttataatc ttctggaaaa
     1561 ttaatttccc cttgagttgt cacagcttta aaactaggtg ctgcctcgcc tatcaacggc
     1621 attgcatagg tttcttgttc cattttcttc ctcctgtatt ttcgttttct tatttctgcg
     1681 tgtataaaac actgcagttg ttagtatgaa tataccaaag ttagtcttat ctaacagtga
     1741 ttatatcaca gaaaacgcaa aatagatagt tttttttctc tttgaacgtt ttttttaatt
     1801 ctttgtatta aaaaaactta aaaacagtaa attccgaatt ttacgctccc ttgacgaaat
     1861 gtcctattat tcctacaata ctataggaat aaaaaatgtt caaaccagcc atcatacaat
     1921 ctttaaaaaa ttataacaca aaaacctttt tatcagatct tattgcaggt ctcatcgtcg
     1981 gaattgtctc cctaccgctt tctattgcct ttgccattgc aagtggcgca agtccagaac
     2041 gaggtattat cacctcgatt atagcaggga ttataatcgc aatctttggt ggctcgaagg
     2101 tgcaaattgc aggacctacc ggtgcctttg tcattatcat ttacggcatc atagaaaact
     2161 ttggtatgtc gggactcctt gttaccacaa ttcttgctgg cttaatcctt atagccttcg
     2221 gtatatttaa gtttggcggc ttaataaaat tcattcccca tacaattgta acgggcttta
     2281 acgcaggcat agccctaagt atttttacca gccaaattgg cgatttttta ggtttgcaaa
     2341 cgggaaaaat gcccattcag tttatttcaa aaatttctat atacctaaaa acaatttcga
     2401 ccattaacat acatgcgctt ttacttggta ccctttcact tgcaatcatc tttctctggc
     2461 cacgcatcac aaaaaaaatc cctggatcga ttattgttat catagttgcg acagctataa
     2521 gtcagatatt taaactaccg attgaaacaa ttggatcacg ttacggcagt attccatcga
     2581 cgatacctat gccaaagatc ccaaactttt ctttagattt acttgtcagc cttttcccac
     2641 aagcgatatc tcttgctgtt ctcgtttcaa ttgaatcgct catgaccgcg gttgttgcag
     2701 acggtatgac agggaaaaaa cataattcaa acaccgagct catcggtcaa ggcatcgcaa
     2761 atataatatg tcctcttttt ggaggacttc cttccatcgg cgctcttgca agaaccgcaa
     2821 caaatattaa aaacggtgga caaacaccta ttgcctcgat tgtgcactcg attgttttac
     2881 ttgttattat gctttttctt gggcaatttg ttatctttat acccatggct gccctcgctg
     2941 caatcctcat ttcagttgca atcaatatgg ctggtataaa agacattcgt ttccttcttc
     3001 gtggacaaaa atcggacatt tctgttttag tggtaacttt tttagctact gtctttatcg
     3061 atttaactgt agcaattgca attgggcttg tcctcgctgc ctttttgttt atacgaaaaa
     3121 ttattgaagt ttctgcggtt tatacaacaa atgtacaatc acagagaatt ttccaagggc
     3181 ttgacgacga atttaaaaca gacattgaag aaaaagagtt gtctattcca aagggtgttt
     3241 taatatatga aatagaagga ccgctctttt ttggaaccgt gcaaaaattt gaaaacgctg
     3301 tcgcaagggc tgaagttgat tttaaagtcc tcattgtacg cacccgtaga atgatttacc
     3361 tcgatgctga gggacttcac gcattaaatc aagttcaaga attatgcaaa aggcgaaatg
     3421 tacaattgct tatttctgga atacatacgc aaccctatac cctgctcgta aaatctggat
     3481 ttgaaaaaaa actaggtgaa aatgctattt tccaaaagat tgacgatgcc cttgcaaaag
     3541 cagaagagct cgtacaataa aataatcttt ctaagatagt tttttttatg agcgatattc
     3601 tatgcgatag cctttttctg tttctggcaa aaatcgaaat atcttttttt cacagtccaa
     3661 aaattctgtt tcatcatgac ttacatgtaa gatagtcgtt gcactttgtt ttgcaatctc
     3721 ttctatcaaa gctaaaacaa aggaacgctc gctcggggag agggcgtggc agggctcatc
     3781 aaggatgagg agtcgaggtg tttttataac cgaacgcaaa ataagaatag ctctttgcat
     3841 gccgtaagaa agatttgcaa agtttttatc tttacactcg ctcatattgg caagggcaag
     3901 ccaaaaaaga accgcttttt tttcgctttc ctttggcgca tcgtataagc ctatggaatc
     3961 gtaaaatcca gaaagcaaga cattttcaac tgaaatagaa cccaagagtc tgtattctaa
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtaacattca ccttttccat
     4141 gcgaaccaat tcttttttct ctcgacctct ttgattagaa tctatttctt gactcagtgc
     4201 agagcgaata tcgatttctt gtttgggact cgtttcttta tcgaatgaaa aggcttcatt
     4261 tgttttttgc atttctatag catttgaaaa acgcatccga agataagagc cttcggtttc
     4321 aagtaagtct tttagcttcg ctacagtctc aaaatctaag ccgtcgtaaa ggtctattaa
     4381 aataattttt tcaatttttc tacaggcaag gagtaaaaag gctttaaaca aaagcacttt
     4441 tttcatttcg ccagtagata gaaactttaa gcctcggtta agcagttttt ctagggacag
     4501 cacattccat agctcttgga gatgggcttt ttcaatcagt tcatggagct tggattcttt
     4561 ttcaaaacat tcttgcagta aaaaaatctc taaattggtg ccggggtcaa agccaccttc
     4621 catgaattcg ctatcatcat tttttcgctc tctttctata agaaggtgag cttcttcaaa
     4681 cgagaccttg aacaagcctg tatttttttc tattttttta ggattaaaca ttttcatata
     4741 agctatagta gcgacctttt tgagcgagta ggctttcgtg agttccctgt tcaataatcc
     4801 tacctttttc caaaactaga atatgatcgg cattttttac tgtagaaagt ctatgagcga
     4861 tgacaaaaac agtctttccc ttcatcaatt catccatggc tttttctatt tgcttttcag
     4921 tccttgtgtc gaccgagctt gttgcttcat ctaagattaa aacaggtggg ctttttatag
     4981 ccgctcgtgc gatggacaaa agttgccgct gcccctgact taaattggca ccgttttcag
     5041 aaagctctgt ctcatagcca ttttccaagt gctgtataaa aaaatcggca tttgcttttt
     5101 ttgcagaatc gataacttct gcatcacttg cagagagcct tccatagcga atattgtcgg
     5161 caatgcttcc agagaataag gcaacgtctt gtaaaacaac agagatagat cgccgtaaat
     5221 ccgcctttcg tatatttgtg atagctatgc catcgtacaa aatacttcct tcgggcgctt
     5281 cataaaaacg atttataaga ttgataatcg ttgtttttcc agagccagtc gaaccaacca
     5341 aggctatttt ttctccggga cgagcatgta aacttaaatc gtgaagaatc gtcttatctt
     5401 ctctataacc aaagtttaag tccttaaaaa tgatttcgcc ttttactttt tcaaaacgcc
     5461 aacttccatc gctttctgga atcttccata agtactcgcc cttattttct tcagatttta
     5521 cgattgttac ccttccctca tcgatttcag catcttcatc gataacagca aatattcgct
     5581 cgcttcctgc aagggcattt aaaacagaat taaactgctg cgacatttgc gttatggggt
     5641 ttgcaaaatt tcgagagtat tgtaaaaagg cagcgacact gcccaagtcg agatgtccca
     5701 gtataacgaa acttgcaccg gcaatcgcag tcagggcata gtttaagtag gaaaggctat
     5761 tcatcatcgg catcaatata ctcgcgtagg cttgcgccct cgtccctgac tttcgtaggt
     5821 tttcattaaa aacagcaaat tcttccttta ctttttcttc tcttgaaaaa accttcacaa
     5881 gtttttgccc ttcaatgagc tcttcaatga agccattcac cttccctagt tctgcttgct
     5941 gttgccgaaa ggcctttccg cttttactcc ctattttagc taccgtaaaa atcataatgc
     6001 ctatcattaa aacaataagg ccagttaaaa tgggactgag cacaagcatc ataataaaag
     6061 tactgacgat ggtgagcata gatgaaaaaa gctgtggcag ggtttgcgag agcatattcc
     6121 gcaatgtatc tatatcgttc gtatacaaac tcatgagatc gccatgtgtt tttccgtcgt
     6181 aaaagcctat gggcaaggtt tccatctttg caaataaatc cacgcgaatc ttatataagg
     6241 tttttgtaga aagatggagc ataatacgac tgtttacata acttgcgagg gctgcaaaaa
     6301 ctgcccctat tgcaacaaca aaaagtatac gtgcaaaatg cgataaatct ggattttgtt
     6361 gaccgattag tggaagaagt acgctgttga taagaggttt gagcatgtaa accagtataa
     6421 tcattgctga tgaactgaga ataatacaaa aaaagacaat tgccaaaagc caggcagaac
     6481 ccttcatata ggaaaaaatt ctcataaggg ttttgaaggc gttttttggt tttgcagcag
     6541 gccccccaaa accatgaccg cgccctcttg gtccgggacg catagtcatt gaaacgctat
     6601 ttttattgtc tgttttattg tttattttat gttccgcttt attttctatt tttttttcac
     6661 tcatccttat tcctcctcat ccaaatcgat aagggcgcca gcctgttgcg attcatatac
     6721 ttcccgataa atagctgatt tttctagcaa gtcctcgtgc cggccttgtg cagaaagctt
     6781 cccctcatcc ataacgagaa tgaggtccga ttctttgatg gaatctattc tctgagcgat
     6841 aataatcttt gttatatctg caaagccttc ttttaaggcg cttcgaatag ccttatcggt
     6901 tgcggtatcg acggcactcg tactatcgtc caagataaga atttttggtt ttttcagcaa
     6961 agcgcgcgca atgctcaatc tttgcttttg cccaccgctt aaattcacgc caccctgacc
     7021 gagctttgtt tcatagccct ctggaaaatt gctcacaaag tcatctgctt gcgctatttg
     7081 acagactttt tttatttctg cgtcgctcgc attttcattt ccccatttta gattttcctt
     7141 gatagtacct gaaaacaagg tgtttttttg caaaaccatc gcaacctgtc cgcgcaattc
     7201 tttcaaagga atatcgcgca catcaatacc gcctactttt acggctccag agagcacgtc
     7261 ataaagtcgt ggaataagtt gcaccaagct cgtctttgaa gaacccgttg aaccaataat
     7321 tccaaccgtc ataccagaat caatctctat attaatatct tttaggatac atttatcttt
     7381 tttatttgaa taactaaaaa acacctcgct aaactgaatg cttccatctt ttatatgcag
     7441 cgttttagtt ttttcgtttt ctacttctat gcaatctttt aggtctactt ctgcatcgag
     7501 cacttcgaca atcctttgca cgctcgctcg tgaaataaca agcattacaa aaatcatcga
     7561 aatcatcata agtgaaaaaa gcacttgcga cagatagcta ataaagctaa tcagctcgcc
     7621 ggtaagtaag ctttcattta cgatcatatt tccgccaaac caaaaaatag ctataatgga
     7681 aagatacata actaactgca tgaggggcat gcccagaatg atgagttttt cagcggaaac
     7741 ttgcgtgctt cgcacctgat cagccgcttc ccgaaatttt ttattttcat gatcttcgcg
     7801 cacaaaagtt tttacaacac gtatggcact gacattttct tgcacacgtg catttaaggc
     7861 gtcatatttt ttcatcatga ggctaaaccg tggaaaggct tttcgcagaa taaaaatcat
     7921 aggtataaaa ataaatggta tcgcaacaaa aaaaactcgt gcaagacttg ggtttatata
     7981 gaaactcata attgtcgctg aaacaaacat cactggtgct cgaacaaaca tacgaagcgc
     8041 catttgatag agttgctgta catttgttac atcagttgtt aaacgcgtaa ccagcgaggc
     8101 agtagaaaag ctgtcgatat ttgcaaatga aaaatcctgt acctttgaaa aaagtttttt
     8161 ccgcaaattc ttcgcaaacc ccaaggaggc aaatgaggca aaaatgccag aaagcgatcc
     8221 gaagacaagg gctaaaagag ccaaactaac catgatgata ccaaggcgcg caacaagacc
     8281 gaggttttga ctcgctatgc cttgatctat tatttttgcc ataagaagcg ggatcaaaac
     8341 ctctaaaaca acttctacaa ttatagcgag gggtgctaaa atacttgtta acacaaagcg
     8401 tttttctaag ccaccaatga gttttttaaa tgatttcatt ctttaaatat gctgaaaata
     8461 tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntaccatca aagacaaaac
     8581 ctgttcctct aacagaagct gttgcaacag cagattttac cgtaaagttt acaacttctt
     8641 gctttgcccg tggtttaact tcaacttgca ccttgccaga cacaagattt aattctgttt
     8701 gaacttcatc ttgtgtttta cgtaattcat ctagactcat tcgtgtaagt ggttttacgt
     8761 gaagtaaaga atcatttata acaatcaccg cagtactctt aaaactcgtt gaaataatgg
     8821 tgccctttgc taaactcata ttttctttta cggcactcca acttgttcca tcagtactat
     8881 attctacttt tcccgtaact gattttactt ttgcagtttg tgcaaagcta taagacacaa
     8941 gcacaaagca taacaaaatt gtagttaaaa gtatcttttt tgttttcatt tataccctcc
     9001 tcattggtat ttttcaaaaa aattaaataa atagacttgc tgtcaaggat accttaaatg
     9061 gattcaatga tgtatcttct acaaggtctt tattaaacag cataatgcct ccagttaaac
     9121 ctaaagaaaa gtcgcttgtt ggaatcaaac tacaattaaa aacgagttca cttcccacat
     9181 accttccctc ttttgttgga tctacaaagg aaaaagcatt aggagattga gaaaaataat
     9241 tatgaaattt tgctgaaaga agaaccgtag aagaaaggct catatttgct gttaaggtaa
     9301 gagattgcaa acttgttgct gttatagctg tttctacaaa cgaaagtggt ttttcagtaa
     9361 tagctttaaa tgacccaccc gagtcgtgta aagcaaaggg gaacaaacct tcaagagata
     9421 attgcccctt aatagttgga atagctaatc ttagatttgc atcaactaaa acataacttt
     9481 ctattgtatt gtagacgttt gcaataccat agacagatac taaatcatag tctagtgaag
     9541 gaataatagg accatttaaa ccaagactta gataataaga attaactgag tcttctccac
     9601 tactgtttaa atcattttgc ccagtaaact ctaaactgac attttgccta aatgcaagt
//
LOCUS       DAOSSW010000070         9344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_7089_length_9344_cov_8.114759, whole genome shotgun sequence.
ACCESSION   DAOSSW010000070 DAOSSW010000000
VERSION     DAOSSW010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9344
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7089_length_9344_cov_8.114759"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PKW26_06500"
     CDS             complement(<1..751)
                     /locus_tag="PKW26_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ93251.1"
                     /translation="MKKRIVFLLIAVGLVTFMFASGRKDGQNQAKTTEVKISVLNGPS
                     GVGLVPLFEREAEKNNLSIQMEVSSTPDVLLPKLIKGEIDMGILPPNAAAKVYNTSEN
                     IVLLAVVGKGMLNLMSLDTNIKTLDNLKGKRVFVAGQGSTPEYLLRYLASQSGIAIDS
                     KQADSLELDFSIPTAELAAALASEKISYAFLPEPFASLAQINNPHLIRALDIQGLYQS
                     FTNTQDSYPMTVLVGRKSFVEENKDLVKKVLK"
     gene            complement(804..3392)
                     /locus_tag="PKW26_06505"
     CDS             complement(804..3392)
                     /locus_tag="PKW26_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOQ93252.1"
                     /translation="MSELSQRAIRLIDSLAQKEASEHWTGSVLPEHVLLALINSKEGV
                     GFEILKKIHIDIESLKSNLIKSFFLSAETPSVGKIPLSRRSSTLFNVAALEARSLQSA
                     YIGTEHILLASLREEKAAIRLFFQENLIPFDEIRKLAVELQRAEKENSKNYTYQLDQN
                     NILFTGPSFNQGRYQQNQKQNQNQQASFLNEFSRDITQKALDGEIDPVVGREKEIQRV
                     VQILSRRTKNNPILVGEPGVGKTAIVEGLAQRIVAKTVPQHLLNKKIYTLDLASVIAG
                     TKYRGEFEERLKRIMKEIKERKNIILFIDELHTIIGAGGAEGTMDASNMLKPALSRGE
                     LQCVGATTLKEYRKYFEKDAALVRRFQQVIVDEPSEEETIEILEGLKKQYEDYHQVRY
                     DDDVIPAITRMAKRYITERFLPDKAIDILDEAGAMKKLTEENRPIELAELEDKIAELN
                     EEKKESVTKQDYEEAARIRDEVRILKQKFEMISSAWQSPDLIERKRVDMQDVCKTLAV
                     MTGLPKEQLSSSDAERLLQMEEEIHKSVVGQEHAIKLISSAVRRSRAGVSSGKRPLGS
                     FIFLGPTGVGKTLLAKTLAKFLFGNEDSLIRIDMSDYMEKHNSSRLVGAPPGYVGFEE
                     GGLLTEKVRRNPYSIVLLDEIEKAHKDVFNLLLQILEEGEIRDSLGHVVNFRNTIIIM
                     TSNAGAREISNENRLGFSFSKEGLLSAEEIKMSATAELKKIMSPELLNRIDDIVVFEV
                     LTEAEIAAIFDIEFYELQKRILDLHIHLEISANAKKYFIKNGYDPVFGARPMRRLIQK
                     EIEDVLATKIIEGSFSTGDQVRVELRGEALMLKIIQKTPSTSKDLLEVETFIETSSEE
                     KEITKV"
     gene            complement(3408..4517)
                     /locus_tag="PKW26_06510"
     CDS             complement(3408..4517)
                     /locus_tag="PKW26_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93253.1"
                     /translation="MTKLSSFDSDKNMGEAWYAYAGPENDVVLSTRISLRRNLENYAF
                     PNAFKFDDAERIQALVLDSFSKIPQAEQYSTMAVKNLEGLGQRILMERGIIHGSIMSE
                     PMASLFIRSDGKLSGTVNVGDHVRLVSFVSGFNLEESFSLAKDLDDALQEHLQFSCDL
                     DFGYLTANIFESGSGMKTSVLLHLPSVYASGLLDKVFKEILANNFAISGFYGGGRKDG
                     SSLGSYYVLSSNNSLQGSEQTQIEEFSLYVNQLIDLERKLRKKLTDNEPTRLRDSIYR
                     ALATVKFARYISANEAIDLISRIKWGANLGILSGINDADLLALLFRTQTAHLQYILQT
                     SSIKYEKDVRTDEQKIERLRSLLIQEAASKIQLIT"
     gene            complement(4510..5043)
                     /locus_tag="PKW26_06515"
     CDS             complement(4510..5043)
                     /locus_tag="PKW26_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOQ93254.1"
                     /translation="MKCDVCKKNEAIIFVRNISGQLDVELHLCHECAKTRNVAIPGKN
                     KAVSLSSLLSAEIQNALNYSPGTKLCSSCGQSALSLSQTKRAGCAHCYTSLKKEILSL
                     LKNENIHPSYIGSISKQHEDFSSNLNERLRLQSNLERAVESEDYEQAAYIRDKLKILE
                     EEAFSKSSGAEKGFSHD"
     gene            complement(5058..6386)
                     /locus_tag="PKW26_06520"
     CDS             complement(5058..6386)
                     /locus_tag="PKW26_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ93255.1"
                     /translation="MSFASSFLFARKILFSNKTQVSQAQKNLFAAFLGLALSLVPLIL
                     VLVISNGMIEGITNRIIGLSSYHIQVQHRIPSYEANPNAHLQDLFTLQKDVLSVDGVR
                     SAHVEVQGIALGTGKKKRTGITVRCVEDTLFSENPSFASYLKLEEGLLEFSSDRSVFI
                     GTKLAKELDLAVGDSFYIISAKNMTSGTVQPNLSVFKVAAIVSSGYQELDALWVYVPL
                     SNAYRIFTSQTSQAILGIELEDPFSHDIYRISNEIESFLTPLEFSFLWSELNREQFEN
                     FSSTKQLLLFVMCIIVLVASVNIASALTSLVLERRREIALLLATGVSQRTIAFSFLCL
                     GSLVGLMGLIVGIPIGLLFSLHINEIIVFLEKLLNFFSGFWYTIVEGAPVVQKHLLDP
                     EFYLETIPIVIPYWDLFLIAVLSLALSVIVSILPSLRSAKEKPLSVLRKL"
     gene            complement(6383..7114)
                     /locus_tag="PKW26_06525"
     CDS             complement(6383..7114)
                     /locus_tag="PKW26_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ93256.1"
                     /translation="MNNKKCDTSFSSFNSDDRVLLKIRDLEKTFQNPASGESLTILKH
                     LDFDVPEQTKIAILGHSGCGKSTLLHIIASLDHPTSGTIFFEETPLHLKDEDALSQFR
                     RHNIGMIFQFHYLLKDFTALENIFLPAYMAGEKKKDAMEKAQSLLVDVGLEKRASHLP
                     SQLSGGERQRVAVARSLINNPKLILADEPTGNLDPENAELISQLLFDMVDQYKKSLVL
                     VTHDMGMSEYCDRVYTIKNARLETL"
     gene            complement(7107..8444)
                     /locus_tag="PKW26_06530"
     CDS             complement(7107..8444)
                     /locus_tag="PKW26_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ93257.1"
                     /translation="MMKRSKRHLEWIFFVSRRFSKLEKESRFAALSILPKIGIAVGVL
                     SLIVILSVMNGFQLESINSILEVSSYHVQASVSEHFESADSESNLSYETIERTKVALA
                     ALPQVQLIQPFLESQALLVGKAGRQSPARIRALSQDAYHGDKGFQNTLKIYSGKFDLE
                     SPSSIVLGSGLAQALNVRVGDTINILALAGSADVDLFSNERLYVVRGLFYTGYGEINT
                     YNAFLSFDEGRALFGPSAELRYGIKLKDSNHAAKLIAQYEKTLPSFHFLSWESFHTSF
                     FQALRLEKNVLLVLVLLIFFVVAINIYNGMKKMVFERKEEIAVFHALGSSPFSIQAIF
                     ILNGLETGFVGSSLGLLIGLFMTNNIDFIFILIGKVQYAVQYIFLFFSSPASLALLKD
                     NSLYTFYASIPAQAYLSELIFISLFGLLSAVLAAWAASRTILRFSIAEVLRDE"
     gene            complement(8428..9192)
                     /locus_tag="PKW26_06535"
     CDS             complement(8428..9192)
                     /locus_tag="PKW26_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93258.1"
                     /translation="MMQSKKNRFIAFFSLIFVYLLGSQESLLDSKNLLRSHLLEDEAL
                     YFTKEQAGAMQSIILQYDSRVIVKKYDALAREFQRNIYEQSDTEDILLASEAFFYMDD
                     SLVAYKKIGIDYEGDLYFESLYDEKGRLMSLEKKAYDKVKKEAGKSLVKESYLWDEKD
                     RLIQKEEVFEKSRLRYENSYEKSFSNEFIYDQKKFLNDSLQEEKIYTDASSYINIHYF
                     SNMLKTFSFYTDGVKTEEIFYQADTVLRRVLYDEAQ"
     gene            complement(9189..>9344)
                     /locus_tag="PKW26_06540"
     CDS             complement(9189..>9344)
                     /locus_tag="PKW26_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOQ93259.1"
                     /translation="LTKYDSSAKGGVVISLGKDLGLPLAFITKGERYTDIEPFAKSQF
                     LDEFIGA"
BASE COUNT         2898 a         1941 c         1666 g         2839 t
ORIGIN      
        1 ctttcaagac tttttttacc aagtctttat tttcttctac aaaagattta cgaccaacca
       61 aaaccgtcat aggataagag tcttgcgtat ttgtaaaact ttggtacaaa ccttgaatat
      121 cgagggctcg tataagatgc gggttattta tttgtgcaag gcttgcaaag ggctctggta
      181 aaaaagcata ggagattttt tcagaagcaa gggctgcagc caattccgca gtcggtatag
      241 aaaaatcgag ttccaaagaa tcagcttgtt tagaatcaat tgctattcca gattgggagg
      301 caagatagcg aagtagatac tctggtgtag aaccttgacc tgcaacaaaa actcttttac
      361 cctttaagtt atcgagcgtt ttaatattcg tatctaggct cataaggtta agcattccct
      421 ttccaacaac tgcaagaagg acaatatttt cactcgtgtt atagaccttt gctgcagcat
      481 tcggaggtaa aatccccata tctatttcac ccttaataag cttcggtagt aaaacatcag
      541 gcgttgaaga gacttccatt tgaatagaaa gattattttt ctctgcctct ctttcaaaaa
      601 gtggcactaa gccgacaccg ctcggtccat tgagaacaga gatttttacc tctgtagtct
      661 ttgcttggtt ttgcccatcc tttcttccgg aagcaaacat aaatgtcact aatcctaccg
      721 ctataagtag aaacacaatt ctttttttca tttttttctc ctttataaag tcttttagaa
      781 agattttata tattgttata aatttaaacc tttgttattt ctttttcttc tgagctagtc
      841 tctataaaag tctcaacttc aagcaagtcc ttagaagtag aaggcgtctt ttgaattatt
      901 ttaagcataa gcgcctcgcc tcgcaactca actcgcactt gatcgcccgt agaaaaactg
      961 ccttcgataa tcttcgtagc aagcacatcc tctatttcct tttgaataag acggcgcatt
     1021 ggacgagcac caaaaacggg gtcatagcca tttttaataa aatatttttt tgcattcgcg
     1081 cttatttcta agtgaatatg caaatcaaga atacgctttt gcagttcata aaattcaata
     1141 tcaaagattg cagcaatttc tgcctcagtt aaaacttcaa agacaacgat atcatcgatg
     1201 cgattgagaa gttccgggct cattattttt tttagttcag ctgtcgctga catttttatt
     1261 tcttcagcag aaagtaagcc ttcttttgaa aagctaaagc caaggcgatt ttcatttgat
     1321 atctcgcgtg ctcctgcatt acttgtcata ataataatag tgtttctaaa gtttacgaca
     1381 tgccccaggc tatcacgaat ttctccttct tctaggatct gcaaaagaag gttaaataca
     1441 tctttatgtg ctttttcaat ttcatcaagt aaaacaatag aatagggatt tcgtcgcact
     1501 ttttcagtaa gaagaccacc ttcttcaaaa ccaacatagc caggaggcgc accaacgaga
     1561 cggcttgaat tatgcttttc catatagtcg ctcatatcga tgcgaataag ggaatcttca
     1621 ttgccaaata aaaattttgc aagcgttttt gccaagagtg tttttccaac tcccgtcggt
     1681 cccaaaaaga taaatgaacc caagggacgt ttaccagaag agacacctgc cctgctccgt
     1741 cgaaccgcag atgaaataag ctttatcgca tgttcttgcc cgacaacaga tttgtgaatt
     1801 tcctcttcca tttgcaataa gcgttctgca tcgctactcg acaattgttc tttcggtaaa
     1861 cctgtcataa cagcaagtgt tttgcaaaca tcttgcatat cgacccgttt ccgctcgata
     1921 aggtcaggac tttgccaggc agaactgatc atttcaaact tttgttttaa aatcctcacc
     1981 tcatctcgaa tgcgggctgc ctcttcataa tcttgttttg ttacagattc ttttttctct
     2041 tcattgagct ctgcaatttt atcttccagt tctgccaatt caatgggtcg gttctcctct
     2101 gtaagttttt tcatcgcacc agcttcatct aaaatatcga tagccttgtc tggcaaaaag
     2161 cgttctgtta tatagcgctt tgccatacgc gtaattgcag ggataacatc gtcgtcgtag
     2221 cgcacttgat gatagtcctc atactgtttt ttgagacctt caaggatttc tattgtctcc
     2281 tcttcgctcg gctcatcaac gattacttgt tgaaaacgtc gcacaagtgc agcatctttt
     2341 tcaaaatatt ttctatattc ttttaaggtt gtcgccccaa cacattgcag ttcccctcga
     2401 gaaagcgctg gcttgagcat gtttgaagcg tccatcgtac cttctgcgcc acctgcgccg
     2461 atgatagtat gcaactcatc gataaaaaga ataatatttt tcctttcttt gatttccttc
     2521 ataatgcgtt tcaaacgctc ttcaaattca ccccggtatt ttgtcccagc tataaccgat
     2581 gcaagatcga gggtgtatat ttttttatta agtaagtgct ggggaactgt ttttgcaaca
     2641 atcctttgtg ctaaaccttc aacaatcgct gttttcccta cccccggctc cccaaccaat
     2701 atgggattat tttttgtccg ccgcgaaaga atttgcacga ctctttgtat ctctttttct
     2761 cgaccgacca cagggtctat ctcgccatct agagcttttt gcgtaatatc gcggctaaac
     2821 tcatttagaa acgatgcctg ctgattttgg ttttgctttt gattttgctg atatctgccc
     2881 tgattaaatg aaggacctgt aaaaagaata ttattttgat ccaactgata tgtatagttt
     2941 tttgaatttt ctttttcagc tctttgcaac tccaccgcaa gtttacgaat ttcatcaaag
     3001 gggatgagat tttcttggaa aaagagtcga atagctgctt tttcttctcg aagagaagca
     3061 agtaaaatat gttctgtgcc aatataagca gattgtaaag aacgagcttc caaggctgca
     3121 acgttaaata aggttgaaga tcttcgcgaa agcggtattt ttcctaccga gggggtctca
     3181 gcactcaaga aaaaactttt aatgagattg ctttttaaag attctatatc aatgtggatt
     3241 tttttcaata tttcaaaacc cacaccttct ttactgttta tcaaagccaa aagaacatgc
     3301 tcaggaagga cggagcctgt ccaatgctcg cttgcttcct tttgggctaa agaatcgatt
     3361 aaacgaatag cgcgttgtga aagttcactc atcaataacc tccaagttta ggtgattaat
     3421 tgtatttttg aagctgcttc ttgtatgagc aaggaacgca gcctttcaat tttttgctca
     3481 tcagttctta catctttttc gtattttata ctacttgttt gtaagatata ttgcaaatga
     3541 gcagtctgtg ttcgaaataa tagcgctaac aaatcggcgt catttattcc agataaaatg
     3601 ccaaggtttg ctccccattt tatacgcgat attaaatcta tcgcttcatt ggctgaaata
     3661 tagcgggcaa atttaaccgt tgcaagtgcc cgataaatag aatctcgaag ccttgttggt
     3721 tcattatcgg ttaatttttt tcgcaattta cgttccaaat ctatgagctg attcacatac
     3781 agggaaaact cttcgatttg tgtttgctca cttccttgca aagaattatt tgagctcagc
     3841 acatagtaag agcccaggga agagccatct tttctccctc ccccataaaa gcccgatata
     3901 gcaaaattgt tcgcaagtat ttctttaaaa accttatcca aaagtcccga agcatatact
     3961 gagggaagat gtaaaagcac agaggttttc ataccgctac cactctcaaa aatatttgcg
     4021 gtaagatagc caaagtctaa atcgcaggaa aattgtaaat gctcttgcaa ggcatcgtct
     4081 aagtcttttg caagtgaaaa tgactcctct aaattaaaac cactgacaaa agaaacaagt
     4141 cgcacatggt cgccaacatt taccgtgcca gaaagtttgc catcagaacg gataaacaaa
     4201 gaagccatgg gttctgacat aatagaaccg tggataatac cacgctccat aagaattctt
     4261 tgtccgagac cttctaggtt tttaacggcc atagtagaat attgttctgc ctgaggaatt
     4321 ttagaaaaac tatcgagaac aagggcctga atgcgctctg catcatcgaa cttaaaagca
     4381 tttggaaagg cgtagttttc taaatttcgc cgtaaactaa tacgtgtcga taacacaaca
     4441 tcattttcag gaccagcata ggcataccat gcttctccca tatttttatc agagtcaaaa
     4501 gaggaaagct tagtcatggg aaaaaccttt ctctgcccca gatgattttg aaaaagcctc
     4561 ttcctctaag attttcaatt tatcgcgaat ataggccgct tgctcataat cttcgctttc
     4621 cacagctcgc tctaaatttg actgaagtcg aagtctttca tttaagtttg aagaaaagtc
     4681 ctcatgctgt ttagaaatac ttcctatata ggatgggtga atattttcat tttttaagag
     4741 agagaggatt tcttttttaa gagaggtata acaatgcgcg cagccagcac gttttgtttg
     4801 actcaaactg agggcagatt gcccacaact tgaacagagc tttgtgccag gagaatagtt
     4861 caatgcgttt tgtatctccg cgctcaataa ggagctgaga gaaaccgcct tgtttttacc
     4921 tggtattgca acatttcgcg tttttgcaca ttcatggcat aaatgcaatt cgacatccaa
     4981 ctgccccgat atatttctta caaagattat tgcttcgttt tttttgcaga catcacattt
     5041 catagcccac ctaaacctta tagctttcgt aatactgaca aaggtttttc tttggcagat
     5101 cgaagggaag gtaaaataga aactattaca gaaagagcaa gactaagcac tgctattaaa
     5161 aataaatccc aatagggtat tacaattgga atagtttcca aataaaactc tggatccaaa
     5221 agatgctttt gaaccacagg tgcaccttcg actatagtat accaaaatcc agagaaaaag
     5281 ttaagcagtt tttccaaaaa gactataatt tcatttatat gcagggaaaa aagaagtccg
     5341 atgggaatac cgacaatgag ccccatcagt ccgacaagag aacctaggca taaaaaagaa
     5401 aaagcgattg tccgctgcga aacccccgtt gcaagcaata aagctatttc acgacgacgt
     5461 tctaaaacta aacttgttag agccgaggca atattgaccg aggcaacaag cacaataatg
     5521 cacataacaa agagcaataa ttgcttagtt gacgaaaaat tttcaaattg ctcgcggttt
     5581 aactcgctcc acaaaaaact aaactcaaga ggcgtcaaaa aagattctat ttcattggat
     5641 attctatata tatcatggga aaaggggtct tcaagctcta tacccaaaat agcttggctc
     5701 gtttgactgg tgaaaatacg gtaggcattc gaaagaggta cataaaccca gagcgcatca
     5761 agttcctgat aaccacttga aacaattgca gcaaccttaa aaacactgag atttggttgc
     5821 accgtgccag atgtcatgtt tttagctgag ataatataaa aagaatcccc aacagctaaa
     5881 tcaagttcct ttgcaagctt tgttccaata aaaaccgaac gatccgagct aaattctaaa
     5941 agcccttctt caagctttag atatgaggca aaggatggat tttccgaaaa aagtgtatcc
     6001 tcgacacaac gaaccgtaat gcccgttcgc ttttttttcc ctgtcccaag agcaatccct
     6061 tgcacctcca catgggctga acgaacccca tcgacagaca aaacatcttt ttgtaaagta
     6121 aaaaggtctt gtaagtgtgc atttggattt gcttcataac tcggtatgcg atgctgcact
     6181 tgtatatgat aacttgaaag cccaataata cggtttgtaa taccttcaat cataccattt
     6241 gaaataacta aaacaaggat gagtggcaca agactgagcg ctaaacctaa aaaagcagca
     6301 aaaagatttt tttgcgcctg cgatacctgg gttttatttg aaaagagaat ctttcttgca
     6361 aataaaaatg aagaagcaaa actcatagtg tttccaaccg agcatttttt attgtgtaaa
     6421 ctctatcgca atactcgctc atacccatat cgtgcgttac caacacaagc gattttttgt
     6481 attggtcaac catatcaaat aaaagctggc taataagctc tgcattctct gggtcaagat
     6541 tccctgttgg ttcatccgct aaaataagct taggattatt tatgagggag cgagctacag
     6601 caacccgttg tctctcccct ccagaaagct gagaaggcaa atgtgatgcg cgcttttcta
     6661 agccgacatc gacgagcaaa ctttgtgctt tttccatggc atctttcttt ttttctccag
     6721 ccatgtaagc aggtaaaaaa atattttcca aggcggtaaa atcttttagc aaatagtgaa
     6781 actgaaaaat catcccgata ttgtgacgcc gaaactggct caaggcatct tcatccttga
     6841 gatgcaaagg agtttcttca aaaaaaatag ttccagaagt gggatgatct aagctcgcta
     6901 ttatatgcaa gagggtactt tttccacaac cactatgacc gaggatagca atttttgttt
     6961 gttcaggtac atcaaagtct aagtgcttta aaatcgtcag ggattctcct gaagcaggat
     7021 tttgaaatgt tttttcaagg tcgcggattt tcaaaagcac ccggtcatct gagttaaagc
     7081 tagaaaaaga tgtatcacat tttttattat tcatcgcgta aaacctccgc tatagaaaat
     7141 cgtaaaattg tccggctcgc tgcccaagct gcaagaacag cagaaagtaa gccaaataaa
     7201 gaaataaaaa taagttcaga caagtaagcc tgtgctggta tagatgcata aaaagtatag
     7261 agcgagttat cctttaaaag tgcaagcgat gcaggcgagg aaaaaaacaa aaagatatat
     7321 tgaacagcgt actgtacttt tccgattaag ataaatataa aatcgatatt gtttgtcatg
     7381 aacaagccga ttaaaagccc cagactgctt cctacaaagc ctgtttccag gccatttaaa
     7441 ataaatatag cttgtattga aaaaggggaa gagccgaggg cgtgaaacac cgcaatttct
     7501 tccttgcgct caaaaaccat ttttttcata ccattataaa tatttatagc aacaacaaag
     7561 aaaatcaata aaaccaaaac gagcaaaaca tttttttcta aacgaagggc ttgaaaaaaa
     7621 gaagtgtgaa aggattccca ggaaagaaaa tgaaaagatg gtagagtttt ttcatactgc
     7681 gcgatgagct ttgcggcatg atttgagtct tttagcttta taccatagcg caattctgca
     7741 gaaggtccaa aaagcgccct tccctcatca aaggaaagaa atgcgttgta ggtatttatt
     7801 tctccgtagc cagtataaaa caaaccgcgc accacataga gcctctcgtt agaaaataaa
     7861 tccacatcgg cagaacccgc caaggctaag atatttattg tatcaccaac gcgaacattc
     7921 aaagcctgtg ccaaacctga accaagcact atagaagaag gactttctaa gtcaaacttc
     7981 ccagaataaa tttttagcgt gttttgaaag cccttatctc catggtacgc atcttgtgaa
     8041 agcgctcgta tacgggcagg actttgtctg cctgccttcc caaccaataa agcttggctt
     8101 tctaaaaaag gctgaatcaa ttgcacttgc ggcagtgcag ctaaagcaac tttcgttcgt
     8161 tcaattgtct cgtaggataa attcgactca ctatctgctg attcaaaatg ctctgataca
     8221 gaagcttgca catgatagga gctcacttct aaaatactat ttatagattc aagctgaaaa
     8281 ccgttcatca ccgataaaat aacgattaag gataatactc caacggctat acctatttta
     8341 ggcaaaatag aaagagcggc aaaacgggat tctttttcta gttttgaaaa acgccttgaa
     8401 acaaaaaaaa tccattccaa gtgtcgttta ctgcgcttca tcataaagaa ctctccgaag
     8461 tactgtatca gcttggtaaa aaatttcttc tgtttttacg ccatctgtat agaaactaaa
     8521 ggtttttaac atattggaaa aataatgtat gtttatataa cttgaagcat cagtatatat
     8581 tttctcttct tgcaaactat catttaaaaa ttttttttga tcatagataa attcattcga
     8641 gaaagatttt tcataggaat tttcataacg cagacgactc ttttcaaaaa cttcttcttt
     8701 ttgaataagc ctgtcttttt catcccagag atagctttct ttgacaaggc tttttcccgc
     8761 ttcttttttt actttatcat aggctttttt ttccaagctc atgagtcttc ctttttcatc
     8821 ataaagcgac tcaaaatata aatccccctc ataatcaata cctatctttt tatacgcaac
     8881 aagagagtca tccatataaa aaaaagcttc agatgcgagg agtatatctt ccgtgtcaga
     8941 ttgctcatag atatttcgct gaaactcgcg agcgagtgca tcatattttt ttactatcac
     9001 ccgagagtca tattgtaaaa taatgctttg catagcacca gcttgttctt ttgtaaaata
     9061 caaggcttca tcttctaaga ggtgggatcg aagtagattt tttgaatcaa gcaagctttc
     9121 ttgacttcca agaagataca caaagatgag agaaaaaaaa gctataaaac gatttttttt
     9181 agattgcatc atgcaccaat aaactcatcc aaaaactgcg actttgcaaa gggctctatg
     9241 tcggtatagc gctcaccctt tgtaataaag gcaagaggaa ggccgagatc cttacccaaa
     9301 cttataacaa ccccaccctt tgcactcgag tcatattttg taag
//
LOCUS       DAOSSW010000071         9270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_7140_length_9270_cov_6.525014, whole genome shotgun sequence.
ACCESSION   DAOSSW010000071 DAOSSW010000000
VERSION     DAOSSW010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9270
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7140_length_9270_cov_6.525014"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1261
                     /gene="aroC"
                     /locus_tag="PKW26_06545"
     CDS             110..1261
                     /gene="aroC"
                     /locus_tag="PKW26_06545"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOQ93260.1"
                     /translation="MAGNSFGKLFKLTTFGESHGEALGCIIDGCPSGIKIDRDTIQNA
                     LNRRRPGYNHSDDKNVTGTTRQEADEVQILSGVFEGFSTGTPICLIIFNARQRSKDYN
                     NIKDVFRPGHADYGYFAKYGIRDYRGGGRASARETAARVAGGAVARALLESMLPKSEL
                     PFSVIAYTKEAAGIACHDIHLSQIEKNQMRAADAWAAEKMLEKIEALKRKGDSAGGII
                     ECQITGLPAGLGEPVFDKLDAVLSHAILSIGAVKGIEFGSGFESARMTGSTWNDPMRE
                     GMKKSEKTDILNPHFSSNNAGGILGGISNGNDIIFRVAVKPVPSIYQTQNTITKEGKN
                     TEILIEGRHDICVCPRIVPVIEAMCYISLADLFLQNKVAKASEIADENE"
     gene            1248..2696
                     /locus_tag="PKW26_06550"
     CDS             1248..2696
                     /locus_tag="PKW26_06550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOQ93261.1"
                     /translation="MKTNKLKLISVISLLSFLVISICLACFYVLSFTPIKTIQDGAQA
                     AFIAEHAKQHISTELEALPYATIPAQLALQTHSVILIDAANDCILYEKNADAIIPPAS
                     MTKLFVMYLVFEAIERGEVNLNEPVPLRPESWAINAPPGSSLMFLGEGQRVSLDELLS
                     GLAVVSGNDAAVAVAQHLSGSVPAFLEKMNALAQSLGLKHTRFVDTSGYSELNQTTAR
                     DFLKFCSIYVKKFPESLERYHSLTSFTYPKEENLKPGLSYSAALQTGTAYNGTLPITQ
                     RATNPLFGKVAGVDGIKTGYIDESGFNLALSIKRDGTRFLAVLMGGPGIGTVQGNQIR
                     TRDVEILTDWAYKTFKSFTISDTIAFNMPLLYGQENSILVYEAINKEKHALSVPLIAH
                     EPSIRRQIAIEPIIKAPVKENEILGYVYYFLGDILLETRPLIAANSVDTGSRFKTSVD
                     GILLPFIAKRLTKAPIDRLQELYRNPQIELQQ"
     gene            complement(2641..4944)
                     /locus_tag="PKW26_06555"
     CDS             complement(2641..4944)
                     /locus_tag="PKW26_06555"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOQ93262.1"
                     /translation="MGKNTMIKRVFYIFCSCFMLFSLSSQVSFSDIDLNAEDRVLFTV
                     SHESYNGQSEKALFLYDLKLPLKQTTSKIEQSNPKILSVYPEYLHYLSKVQSLRIENR
                     YGVFQYRLADKTLEQKELSNEIEGQTERFIPLQNNTAVHRFNSDGSLVCEFEQTSPSK
                     ANLVVKDLYTGKKIVLSTDNDFSFDSLPALWAPLGQGQVQNSSLLVYEKKGRLYFINL
                     KEGLQEKMIDEEYRFIGPGSIQSVFFANEKKLMYIQKDLVYEISTNELYTRALYSDIV
                     GIGKIAGRLPSPFNSSKDRFWTSENGRSIVLLQDNRTLLYMELEGTDFNFVTSLFSYP
                     FVRVPGASLSFDVFWTQSKYETQIPIIWMQILRAGKTESYTWRLKIDTEKNFAFFENL
                     NLPVFVHSPLVSPNKTKLAFMGESMSYVYDINTWKQSQVFTDEKLISLVWKSDTELFL
                     GGENTLRLWSWVKNSSEVLALSSPGDYAWDAQKNILSKFGRKNYLYDKNRGSWQEVSE
                     GNTRSREVQNARWRIFIDEMEQGHFSNSLFARKLSGLSTNIALFNNSKDKPAQKPRVA
                     LAIDALDSSDGLTPILSALHKNNIRASFFINGEFLRKFPTAVKEIASLGHQCGSMFYI
                     PYTLDTRLYAIDETFIRRGLARNEDDFFYLIGKELSLIWHAPDYFSNAIIQSASKKAG
                     YKLIDSPHQTGDTLTLEEATEKNLPYRSSSQIIDSIVMSLKPGLIIPISCGLQNGTRL
                     DYLYDKIDVLIAAILSAGYDIVPVSDL"
     gene            complement(5019..6182)
                     /locus_tag="PKW26_06560"
     CDS             complement(5019..6182)
                     /locus_tag="PKW26_06560"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ93263.1"
                     /translation="MHIISYLDLNTTSSAYKTEPGISSKRQRSLPSSAVYAKAIELKS
                     KFSLDIKKILKIILFIPKVLFRSRFLLLSIAIVFCLSLSFTNVLGKKPLHIRNAVLEK
                     STAGEIASLDSAMTDFALPKKESFDSEGYLGTKNISQSIFTKPVRYQTYTVKAGDTIS
                     GIAKRFNLSNISTLIAINDIKNVRSLRSGQKIRIPSVDGLTHIVKSGESLSGIADKYK
                     TSLVTLLDVNDLTTELVKKGDVLFIPGATMDATALSEALGELFKDPLGVSWRLSSPYG
                     YRPDPFTGVRSFHTGIDMVAPLGTSIKASMAGKVAAAGYNQVYGNYVIVSHSGGYQTL
                     YAHMTTILVKQGQNVNQGSVLGKLGSTGYSTGPHLHFSVYKNGQLINPLSVLK"
     gene            6228..6737
                     /gene="lepB"
                     /locus_tag="PKW26_06565"
     CDS             6228..6737
                     /gene="lepB"
                     /locus_tag="PKW26_06565"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOQ93264.1"
                     /translation="MTKHRVITMLFLGLLIGFILKVFVFDIVKISGTSMQPAIQEGDM
                     VVIWKLAYGLVKPFSPYSFTRWKEPKQGDIIAYLHNNKIVIKRCLATEGMHLSFSSLS
                     GYHVRIQIKETDTVIPLSEKQYQRLKMTTTVPTGFLFAVGDNYEDSIDSRDYGFVAAN
                     EVTGKVLCK"
     gene            6728..8605
                     /locus_tag="PKW26_06570"
     CDS             6728..8605
                     /locus_tag="PKW26_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOQ93265.1"
                     /translation="MQIETRYKKGRLIFFFLCLILFTVYLIYSYAKIAFSPSTERAFF
                     SQEYIRGSIYDRNGKPLAVQASLYHLAATPSAIKDIAKTAKVLAPYLEKTEEEIVTQI
                     NEATIDFVYIEKKLSQEKYEALLSLISSNKLYGLRFDKVQGRIYPEKSLASQVIGFMG
                     NDGKGLSGIEYTMQKILSPDTTKNPTSPIHYGNDIYLTIDANLQYKLEQIALENLEEH
                     QAESIMIIASDAKKGEILSYVSLPSTDLNEYPSAIDAEKIDRPAAISFEPGSVFKIFS
                     IASFLETGAIGANELFYCDGKYEIPSTGGEKAVINCLGHHGWISAREALEYSCNDALA
                     QMSEKIDARHFVQMLRAFGFGSKTLLEVPLEISGSIKSPSDRLWSNRSKPTMSIGQEL
                     SVSAIQMIQATTALANKGYPLQLTYISQIAESDGKLIYQHTPEYKEPVISEATSRTLL
                     DYMKSTAEVGTGSRAQLGDVTIGVKTGTAQMIDPITKGYSKDDYISNCVAIFPIEDPE
                     IILYIVITKAKGETYAGRIVAPIIAEAADIIIDHLGLARKNASSLIHSGKIELPKKIN
                     PEISTIVPNFLGLSKRELVPLLERDDIHFIIRGDGWVSSQNPPPGTPLQENMTIELYL
                     Q"
     gene            8586..>9270
                     /locus_tag="PKW26_06575"
     CDS             8586..>9270
                     /locus_tag="PKW26_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93266.1"
                     /translation="MNSIYNKNIAALKQRLDTLYETHRLLIEAYKEQNAACLRAYEEK
                     ESLKTIPAKNGSLTAVYKELFLHSSYNPEAEAKKSIAEVKEKEQIAFLGFGLGYAPLA
                     FVEAHPDKMLILIEPEIERVLWTLSYVDFSALFSHPKLICLFSAPPEDCISLLEHFGF
                     ENTAFIKNESYALHQRDFFSTLSLLIERNKQKKDINDSTLERFGKLWLKNTIKNIHHY
                     AERRGIACLQ"
BASE COUNT         2893 a         1871 c         1861 g         2645 t
ORIGIN      
        1 cgtttcttag gggaagagaa ccatcttaca ttttttctaa catccgtgtt agaaaaaact
       61 tcctatcaag agtctttcct agaatgcgct tttatggtag tcttttttta tggcaggaaa
      121 ttcatttgga aaacttttta agctcactac cttcggggaa agtcacgggg aagctctggg
      181 ctgtataatc gatggatgcc cgagtggcat aaaaattgac agggatacga ttcaaaatgc
      241 gttgaatcga agacggcctg gatacaatca ctcggacgat aaaaatgtta cgggtactac
      301 tcgtcaagaa gctgatgagg tgcagatttt aagtggcgtc tttgagggtt tttcaacagg
      361 cacgcctatt tgtctcatta tttttaatgc aaggcagagg tcaaaagatt ataacaatat
      421 aaaagatgtt tttcgacctg gtcatgctga ttacggctac tttgcaaaat acggtattcg
      481 tgattatcgt ggtggtggaa gggcttctgc ccgagaaact gctgcgcggg tagcaggagg
      541 tgctgtggca agggcgcttc tagaaagcat gctcccaaaa tcagaactgc ctttttcggt
      601 catagcctat acaaaggaag ctgctggaat tgcttgtcac gatattcatc tcagtcaaat
      661 agaaaaaaac caaatgcgtg cagctgatgc ctgggcggca gaaaaaatgc ttgaaaaaat
      721 agaagcgctt aaacgcaagg gagatagcgc aggtggtatt attgagtgtc agattacagg
      781 gctgcctgca ggtcttggcg aacctgtctt tgataagctc gatgcagttt tatctcatgc
      841 gattctttct atcggtgcgg ttaagggtat agaatttgga tcgggctttg aatctgctcg
      901 catgacgggc tcaacctgga atgatcctat gcgcgagggg atgaaaaaaa gcgaaaaaac
      961 cgacatttta aatccgcatt tttcttcaaa taatgcaggt gggatcctcg gcggcatatc
     1021 gaatggaaat gacattattt ttcgagtggc tgttaaaccg gtcccaagta tttatcaaac
     1081 acaaaacaca attacgaaag aaggcaaaaa cacagaaatt ctcatcgaag gtaggcatga
     1141 tatttgtgta tgcccgagaa ttgtgcccgt tatcgaggcg atgtgctata ttagccttgc
     1201 tgaccttttt ttacaaaaca aagttgcaaa agcttcggag atagccgatg aaaacgaata
     1261 aactaaagct cataagcgtc atctctcttt taagtttcct tgtcataagt atctgtcttg
     1321 catgctttta tgtcctttca tttacaccaa taaaaacaat acaagatggc gcgcaagccg
     1381 cttttatagc cgaacatgca aaacaacata tttctactga gctagaagca ttgccctatg
     1441 cgacaattcc tgcacaactc gccttgcaaa cgcattcggt tattttaatc gatgcggcaa
     1501 atgattgcat cttgtacgaa aaaaatgctg acgctattat tcctcctgct tcaatgacaa
     1561 agctttttgt tatgtacttg gtttttgaag caatcgaaag aggagaagtc aatcttaatg
     1621 agcccgtccc tctgagacct gaatcctggg caataaacgc accaccgggt tcatcgctta
     1681 tgttcctcgg cgaggggcaa agggtgagct tggacgaact tttgtcgggg ctcgctgttg
     1741 tttcaggaaa cgacgcagca gtcgcagtcg cccaacacct ctctggttct gttcccgcct
     1801 tcctcgagaa aatgaacgcc ctcgcccaat ccctaggact caagcatacc cgctttgtag
     1861 acacatcggg ctatagcgaa ttgaaccaaa caacagctcg cgactttcta aaattttgct
     1921 ctatctatgt aaaaaaattt cctgaaagct tagagcgcta tcactcgctg acttctttta
     1981 cctatcctaa ggaagaaaac ttaaaacctg gtttaagcta ttcggctgcc ttacaaacgg
     2041 gaactgcata taacggaacg cttcccataa cgcagcgggc aacaaatcca ctctttggaa
     2101 aagtagcagg cgtcgatggc attaaaactg gatatatcga cgagtctggc tttaaccttg
     2161 ccttgagtat caagcgagac ggtactcggt ttttggccgt tctcatggga ggacccggca
     2221 ttggaacagt gcagggaaat caaatacgaa caagggatgt agaaattctc accgactggg
     2281 cttacaagac gtttaaaagt tttacaatta gcgatacgat tgccttcaat atgcctcttt
     2341 tatatggtca ggaaaacagt attttagttt acgaagccat aaacaaagaa aaacacgcgc
     2401 tttctgttcc cctcatagca catgagccga gtattcgaag acaaattgct attgagccta
     2461 taattaaggc accggtaaag gaaaacgaaa tcctcggtta tgtctattat tttttgggag
     2521 atattttact tgaaacgcgt ccgcttattg cagcaaattc agtggatacg gggagccgtt
     2581 tcaaaacgag tgtagatgga atactgctcc cttttatagc aaaacgactt acaaaagcac
     2641 ctatagatcg cttacaggaa ctatatcgta acccgcagat agaattgcag caatgagcac
     2701 atcgatttta tcatagagat aatcgagccg cgtgccattt tgtaagccac aggaaatagg
     2761 aatgataagc ccaggcttta acgacattac aatcgaatcg attatttgac ttgaagatct
     2821 gtagggaaga tttttttcgg tagcttcttc aagcgtcaaa gtatctcccg tttgatgggg
     2881 agaatcgatg agcttatagc cggctttttt acttgccgat tgtataattg catttgaaaa
     2941 ataatctggt gcatgccaga taagagaaag ctccttaccg atgaggtaga aaaaatcatc
     3001 ttcatttctt gccaagccac gacgaataaa ggtttcatcg atagcgtaga gtcttgtgtc
     3061 gagggtatag ggaatgtaaa acattgagcc acattgatgc ccgagagatg caatttcttt
     3121 taccgctgtt ggaaactttc ttaaaaattc accgttaata aaaaaacttg cccgtatatt
     3181 atttttatgc aaggcagaaa gtatgggagt aagaccgtcg ctgctatcaa gcgcatcgat
     3241 ggcaagggct acccgcggtt tttgagcagg tttatctttt gagttattaa acaaggcaat
     3301 attcgtacta agccctgaaa gctttcgtgc aaaaaggctg tttgaaaaat gcccctgttc
     3361 catctcatct ataaaaatac gccagcgagc attttgtact tcacgacttc gcgtatttcc
     3421 ttcggaaact tcttgccatg aaccgcgatt tttatcatag agataatttt tcctaccaaa
     3481 ctttgaaaga atgtttttct gtgcatccca tgcatagtcc cctggcgatg agagcgcgag
     3541 tacttcggag ctatttttta cccatgacca aaggcgaagc gtattttctc ctccaagaaa
     3601 aagctcagtg tcggacttcc aaacaaggga gatgagtttt tcatcagtaa atacctggct
     3661 ttgtttccag gtatttatat catacacata actcatcgat tcacccataa aagcaagttt
     3721 tgttttattt ggcgaaacaa ggggagaatg cacaaaaaca gggaggttta gattttcaaa
     3781 aaaagcaaag tttttttctg tatcgatttt caaacgccag gtataacttt cagtttttcc
     3841 tgcgcgtaga atttgcatcc agattatggg tatttgagtt tcgtattttg actgtgtcca
     3901 aaatacatcg aaagaaagcg atgcgccagg cactcgcaca aagggatagg aaaataaact
     3961 cgttacaaag ttaaagtccg taccctccaa ctccatgtag agaagcgttc gattgtcttg
     4021 taaaagtact atagaacgac cattttcact tgtccaaaat ctatctttgg agctattgaa
     4081 aggagaagga agacgccctg ctattttccc tataccgaca atgtcagaat aaagcgctct
     4141 tgtgtacaat tcatttgtag aaatttcata gaccaagtct ttttgtatgt acatgagttt
     4201 tttttcgtta gcaaaaaaaa cagactgaat ggaaccagga ccgatgaaac gatattcctc
     4261 atcgatcatt ttttcctgta agccttcttt tagattaata aaatacagcc ttcctttttt
     4321 ttcatagact aagagagagc tattttgcac ttgtccctgc ccaagcggag cccagagagc
     4381 aggtagggag tcaaagctaa agtcattgtc tgtagaaaga acaatttttt ttcctgtata
     4441 taaatctttt accacaaggt ttgctttaga agggcttgtt tgttcaaact cacagacaag
     4501 gctaccatca ctattgaagc gatgtaccgc ggtattattt tgtaagggaa taaaacgttc
     4561 tgtttggcct tcgatttcgt tagaaagttc tttttgttca agtgttttat ctgcaagtct
     4621 gtattggaaa acaccgtatc gattttctat gcgtaaactt tgcacctttg ataaataatg
     4681 caaatattct ggataaacgc ttaagatttt gggattcgat tgctctattt tacttgtcgt
     4741 ttgcttgagt ggcagtttca aatcatagag aaaaagcgct ttttcactct gcccattgta
     4801 gctttcatgc gatacagtaa aaagcaccct atcttcagca tttaaatcaa tatctgaaaa
     4861 agaaacttgt gaactaaggc taaaaagcat aaagcaagag caaaatatat aaaaaactcg
     4921 ttttatcata gtattttttc ccataaacat atactaacat agatttaagc ttttgttcta
     4981 tgtactttta ggtaattaaa aaaacagctt cgttcaattt attttaaaac tgataatggg
     5041 ttaatgagct gtccattttt atacacagaa aagtgtaaat gcgggcctgt cgaatagccc
     5101 gtgctaccca gtttccccaa aacacttcct tggttcacat tttgtccctg ctttaccaaa
     5161 atcgtagtca tatgtgcata gagcgtttga tagccaccag aatggctcac aataacatag
     5221 tttccataaa cttgattata acccgctgcg gcaaccttgc ctgccatcga tgcttttata
     5281 ctcgtaccaa ggggtgcaac catatctata cctgtgtgaa aactgcgcac tccggtaaat
     5341 gggtcaggtc tataaccgta gggagaagac agtctccatg aaacaccgag ggggtcttta
     5401 aataattcac ctaaggcttc actcaaggca gtcgcatcca tcgttgcacc gggaataaaa
     5461 agaacatcgc ctttttttac aagctctgtt gtaaggtcat tcacatcgag caaagttacg
     5521 agacttgttt tatatttgtc agcaatgcca gataaagact cgccagattt tacgatatgc
     5581 gttaaaccat cgacagaagg aatacgtatt ttttgaccag atcgaagaga gcgtacgttt
     5641 tttatatcat tgatagcaat gagtgtagaa atatttgaaa gattaaaacg ctttgcaatg
     5701 cccgaaatag tatcgccggc ttttacagta tatgtttgat atctaactgg ttttgtaaat
     5761 atcgattgcg aaatattttt tgtaccaaga tagccttcgc tgtcaaagga ttcttttttg
     5821 ggtagggcaa agtctgtcat ggcactatct aaggaagcga tttcacctgc ggtacttttt
     5881 tctaaaacag cattacgtat atgtagcggt ttttttccca aaacatttgt aaaactgagg
     5941 ctaaggcaaa aaacaatagc aatgctcagc aataaaaaac gacttcggaa taagactttt
     6001 ggtataaata agattatttt tagtattttt tttatatcaa gagaaaattt tgattttaat
     6061 tctatggctt tcgcataaac agctgaagaa ggcaggcttc tttgtctttt ggaagaaatt
     6121 cctggctctg ttttataggc tgaggaagtg gtatttaaat ctaaataact aataatgtgc
     6181 atactctatg tatcgacatt atggggaaga cttattagaa taaaaacatg acaaaacaca
     6241 gagtcatcac aatgcttttt ctcggcttac tcatcggctt tattttaaag gtctttgttt
     6301 tcgatattgt caagatttct gggacgtcga tgcagcccgc gattcaagaa ggcgatatgg
     6361 tagtcatatg gaaactcgca tacggacttg taaaaccctt tagcccctac tctttcaccc
     6421 gatggaagga accaaaacaa ggcgatatca tcgcatatct gcacaataat aaaattgtta
     6481 taaaaagatg cctcgcaacg gaaggaatgc atctttcttt ttcttcactt tcaggctatc
     6541 atgtgaggat acaaataaaa gaaactgata cggtgattcc gctttcagaa aaacaatatc
     6601 aacgcttgaa aatgactacc actgtaccaa caggctttct atttgcggtg ggcgataatt
     6661 atgaggattc tatcgattca agggactatg gttttgtagc ggcaaacgag gtaacgggga
     6721 aagtactatg caaatagaga caagatataa aaaaggcaga ctcatttttt tctttctttg
     6781 cctcatactt tttaccgtct atcttattta ttcctatgca aaaattgcat tttctccaag
     6841 cacagaaaga gcatttttct ctcaagaata tatacgtggt agcatctatg acagaaatgg
     6901 taaacccctc gcggtgcaag caagtctcta tcatttagct gcaacgcctt ctgccataaa
     6961 ggacatagca aaaacagcca aagttttagc accctatttg gaaaaaacgg aagaggaaat
     7021 tgtcacgcaa atcaatgaag caacaattga ctttgtctat atagaaaaaa aactcagcca
     7081 agaaaaatac gaagcgcttt taagtcttat cagtagcaat aaactctatg gcttacgttt
     7141 tgataaggtg caaggcagaa tctacccaga aaaatcgctg gcttcacagg ttatcggttt
     7201 tatgggaaat gatgggaagg gtctttcggg aatagagtac actatgcaaa aaatactttc
     7261 acctgataca acaaaaaacc ccacaagtcc tattcactat ggaaatgata tttatctcac
     7321 tatagatgca aacctacaat acaaactcga gcaaattgct ttggaaaact tggaagaaca
     7381 tcaagcagaa agtattatga taattgcctc tgatgcaaaa aaaggcgaga tactttccta
     7441 tgtaagttta ccgagcacag acttaaacga atacccaagt gctatcgatg cagaaaaaat
     7501 agacagaccc gcagctatct cttttgaacc aggctctgtt tttaaaatat tttccattgc
     7561 ttctttttta gaaacaggag caataggtgc aaacgagctt ttttattgtg atggaaaata
     7621 cgaaatacca tctacaggag gcgaaaaggc tgttataaac tgcctcggtc atcacggctg
     7681 gattagcgcc cgagaagccc tcgagtattc atgcaatgat gccttggcgc aaatgtccga
     7741 aaaaatagat gcaaggcatt ttgtacaaat gctacgggcc tttggctttg gttcaaaaac
     7801 acttttagag gtgccgcttg aaataagtgg cagtataaaa agccctagcg acaggctttg
     7861 gtcaaatcgt tcaaaaccga ctatgtctat cgggcaggaa ctcagtgtca gtgcgataca
     7921 aatgatacag gcgacaacag ctcttgcaaa caagggctac cccttacaat tgacctatat
     7981 ttcacaaatt gcagaaagcg acggcaagct catctatcag catacgccgg aatacaagga
     8041 acctgttatt tccgaagcga caagcaggac tttacttgac tacatgaaaa gtaccgctga
     8101 agttggcaca ggaagtcgag cgcaactcgg agatgttaca atcggcgtaa aaacaggaac
     8161 ggcacaaatg atagacccca taacgaaggg ttatagtaaa gacgattata tatcaaattg
     8221 tgttgctatt ttccccatcg aagatccaga aattatctta tacattgtta taacaaaggc
     8281 gaagggcgaa acctatgctg gtagaattgt tgcaccgatc atagccgagg cagcagatat
     8341 tattatcgat catcttggct tagcacggaa aaatgcatca tccctcatac actctggaaa
     8401 aatcgagctg ccaaaaaaaa ttaaccctga aatcagcaca attgtcccca actttctagg
     8461 gctttcgaaa cgagaacttg ttccccttct cgaacgcgat gatattcatt ttattatccg
     8521 cggagacggc tgggtttctt cacaaaatcc ccctccggga acgcccctac aggagaacat
     8581 gacgattgaa ctctatttac aataaaaata ttgcagcttt aaaacaacga cttgatacac
     8641 tctacgaaac ccatcgcctt ttaatagaag cctataaaga acaaaatgca gcctgtctaa
     8701 gagcctacga agaaaaagaa agcctaaaaa ccattcctgc aaaaaacggc agtcttacag
     8761 ccgtctataa agaactattt ttacactcat catataatcc agaggcggaa gcaaaaaaaa
     8821 gcatagctga agtaaaagaa aaagagcaaa tagctttttt aggtttcggt cttggctacg
     8881 cccccctcgc ctttgtcgaa gcccatcctg acaaaatgct tatccttatt gagccagaaa
     8941 tagaacgagt tttgtggaca ctttcctatg tcgacttttc cgcgcttttt tctcatccaa
     9001 aacttatatg ccttttttca gcgcctccag aggactgcat aagtctcttg gagcattttg
     9061 gctttgaaaa caccgccttt ataaaaaatg aaagctatgc cttgcatcaa agagattttt
     9121 tttcaacact ttcgctttta atcgagcgaa ataagcaaaa aaaagacata aatgacagta
     9181 ctctcgaacg ttttgggaag ttatggctta aaaacaccat aaaaaacatc caccactacg
     9241 cagagcgcag ggggattgct tgtttacaaa
//
LOCUS       DAOSSW010000072         8766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_7624_length_8766_cov_6.700034, whole genome shotgun sequence.
ACCESSION   DAOSSW010000072 DAOSSW010000000
VERSION     DAOSSW010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7624_length_8766_cov_6.700034"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PKW26_06580"
     CDS             <1..264
                     /locus_tag="PKW26_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HOQ93267.1"
                     /translation="GENKSFDDYFSETVTKIGLKGEEAEIALMSQNAVMSDLRSLRDS
                     ISGVNIDEELADIIKFQHGYNAAAKFISVVDDIINTVINGLRA"
     gene            282..1526
                     /locus_tag="PKW26_06585"
     CDS             282..1526
                     /locus_tag="PKW26_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HOQ93268.1"
                     /translation="MRRVSSGQANLDVQNNLRRQERNLNKVNNQLGSQQRIQSLRDDP
                     IAAGHLVRYQSYAKRIEQFKHNAETISDQFILSEGYVDHSLQIMHRLRELAVTGAHGV
                     YNKDDLANMAAEVDELLKEIIQNANALDADGNALFAGERSKNQAFIVDKGNTTGSDSE
                     LIQRVRYNGSIKQNEVEVDEHAYMSVNRSGNQIFWAESQQLIAQRDATSYTVLEDAVV
                     SINGTDISLKTGDSIHGIIAKINNSGAPVKASLDPMSNALMLHTTDSRQLWLEDIDGN
                     AFEDLGLIKDKSQRPPYNLNDSVLSSGGSVFDTVIALRNAMLNNDSESIGGKILGSLD
                     SAIDNLTSSLAELGSKYERALLNVARSEKSILNVTSQISREGDLDITTAITDMKMLDY
                     VKNATLSTAGKLYNSTLLNYIR"
     gene            1542..1961
                     /locus_tag="PKW26_06590"
     CDS             1542..1961
                     /locus_tag="PKW26_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOQ93269.1"
                     /translation="MSEQSEIFEKKLLIEKGLLGFETITNYSLTHADLEPFMWLKAEG
                     EKNLAFLVVDPFILFSDYEIDVDDNSLSEIDIKNPSDVVVLTILTVSKTGEEPTANLQ
                     GPVIVNKNNNKAIQVVLSDPRWMTKHSITNQLETRGA"
     gene            1961..2194
                     /gene="csrA"
                     /locus_tag="PKW26_06595"
     CDS             1961..2194
                     /gene="csrA"
                     /locus_tag="PKW26_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOQ93270.1"
                     /translation="MLILSRKINEKIKIGEDITLSIIEIKADQVKIGIDAPKEVKVFR
                     QEVFNAIQNENLAAATSTESIESLSNLFAKKEF"
     gene            complement(2223..2912)
                     /gene="tsaB"
                     /locus_tag="PKW26_06600"
     CDS             complement(2223..2912)
                     /gene="tsaB"
                     /locus_tag="PKW26_06600"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOQ93271.1"
                     /translation="MKALAIDGATNTINFLACNGDKKALLSLDLAMKQSEKLLPSIDF
                     VLKEVDLEAKDLEFTAVSLGPGTFTGLRLAFAALKALSLSFQIPLYAISSLVMYAKPF
                     LTLEGLILPAIDAKKNRFYTSFYRNGKKVFDEMDISAKDLVKKLDPEEKIFCVGPDAK
                     MLSDALLAENAALNIQYLSIHVSAASFYLLEMAGELYKAKSPPLMDWEGPFYLRASEA
                     EENKVVLKKEL"
     gene            complement(2909..3340)
                     /gene="tsaE"
                     /locus_tag="PKW26_06605"
     CDS             complement(2909..3340)
                     /gene="tsaE"
                     /locus_tag="PKW26_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOQ93272.1"
                     /translation="MILQSFSSEETIRIGEKIGSHLQKGDIIALQGFLAAGKTTITKG
                     IALGLGIDEDVTSPTFTLISEYYGRLPLYHFDVYRLDSVEDFLNLGSEEMLYGDGVCV
                     IEWSEKILDVFPEHTIYVRLEVNEDDSRSIHLENWTYGEIL"
     gene            complement(3337..3597)
                     /locus_tag="PKW26_06610"
     CDS             complement(3337..3597)
                     /locus_tag="PKW26_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93273.1"
                     /translation="MKILEIKNIVSEENHIYYRRKFSSIAVVQIPQRQIEFPFQFIIE
                     TAPTGNKNIEITIPETIDYPLLPVVRALKHKINQFDAEGYLK"
     gene            complement(3648..4706)
                     /locus_tag="PKW26_06615"
     CDS             complement(3648..4706)
                     /locus_tag="PKW26_06615"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOQ93274.1"
                     /translation="MILLDIGFTRNYSPVIYIPLLIAGGIGLAFFLINIIIRIFQSSF
                     HKKVNDPNRPTTVQDINYVTQILHLTNEEKNFLQELCKDNKCPNFIMFMRNQAKIDAL
                     FKTIIHTTADEKKKAILYSIRTKIEQDPQNTIFITSTKNILEGQRLMYSDENLDHHPS
                     IVLKNTKEGLIVQVPKNIYGDEIKPKPLSKINFSFETKNLIAYTFQTRIVRYQNTREN
                     EMVISHTNNLEVLVRRNQKRVPYKPQCVFSAVKVSSGGLGKNPKVSYEALPKKHEGTL
                     MDISANGCSLQTNMPISKGQYIYIEFRMDAEAIEKAFGKIVNATTTQTKLTILHIEFV
                     KISLETRNKIYKKVYDYV"
     gene            complement(4724..5314)
                     /locus_tag="PKW26_06620"
     CDS             complement(4724..5314)
                     /locus_tag="PKW26_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93275.1"
                     /translation="MQITINKQKTDIELENEKTLGDVLRAFQKSFEENDAALIGLGID
                     DLILHPNDLAAFMEKPLSEISDLNLETILLSDIREALATFTLPLETLSNQFLDIGVLY
                     QANKDTEVSKIITEFADFFSSLSKTISLLSLFPNYFNKTKINDEMILDYLNNFSSILI
                     EFENALANNDKVLVADLAEYEIAPSLETLSVFCKNN"
     gene            complement(5326..7323)
                     /gene="fliD"
                     /locus_tag="PKW26_06625"
     CDS             complement(5326..7323)
                     /gene="fliD"
                     /locus_tag="PKW26_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOQ93276.1"
                     /translation="MADINIPGVSDKYKTNDLIAALVEAERIPLKREQDKMDGYKFEQ
                     KSWQRLNLYMSNLRETSRSLFSFDNPFSEKLSSSSQEFAITATPGRNAALETFKFDVD
                     EIAKADRFLSKPIEKNATIEKGQYSFHVGEKSLSFNWKGGTLDEFIAAVNRRSNGLIK
                     ASLIGVTSSTKSLLVESLQTGAENRLRFSDMALDLALNYELIEAAPSELENFGTSQNE
                     LQRIPGFQNEAKIIEKTILLPPESGFEIQIPKSVKEDPQSLIEFTVHSKTVPDITKDT
                     SPQSSPLFNEQLFAEFKNVTIFNEKIETALTEKEIEQREPVVSDSFVYLKMEDGTRSL
                     ISTEGKEKIRIALSDYPKLSSIIIENKNTGKELSLSEFTSRNPNKIDGYAPVNPISIA
                     QDAQIRYEGIPMTRPTNTIDDIIPDITLHLENPTEKTASIQIKPDEEKAKDALITFVG
                     NYNQLIAEMNILSQANPDIVEELQYLTTEENKEAHERLGLFRTEYTVSNTKSSLQRII
                     TNSYPIELNNTISMLSQIGIASKASSGGSLSNTQLRGYLEIDEKKLDAALKESMGEIK
                     NLFGIDTDGDLVIDSGIAYLIDKQLQAYVTSGGILSMKNASLDTKITSSQKQIDRLEV
                     QIAKKEQELKIKYGNMESALNSLQSQSDSLTNFTNSYNNNK"
     gene            complement(7348..7710)
                     /locus_tag="PKW26_06630"
     CDS             complement(7348..7710)
                     /locus_tag="PKW26_06630"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HOQ93277.1"
                     /translation="MSIMSIGQNSAMDGRSIISTIKPKLAPTERAEDTKKINAEAANV
                     AKEIQKTEAQIRHINETTNIAGRKIQFLVNEALGKVVVKIVDSETDKVIKEIPSADVQ
                     NLQIRLKEVLGLLIDEEI"
     gene            complement(7897..8751)
                     /locus_tag="PKW26_06635"
     CDS             complement(7897..8751)
                     /locus_tag="PKW26_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOQ93278.1"
                     /translation="MIINHNMSSMYANRTLGANNANIAGNIEKLSSGLKINKAGDDAS
                     GLAVSEKMRSQIRGLNQAERNIQNGVSFIQTTEGYLEETTDILQRLRELSIQSANGIY
                     SDEDRMQIQVEVSQLVAEVDRIANHAQFNNMNMLTGAFAFDSAAGRVMQLHIGANTDQ
                     NERIYIGTMTAQALGLVAGQGSDDRISISSPETANSAIMTIDEALKSVNKQRADLGAY
                     QNRFEIAAKGIAVAAENLQAAESRIRDTDMASEIVEYTKNQILTQSGAAMLVQANSQS
                     QNVLKLLS"
BASE COUNT         2574 a         1584 c         1629 g         2979 t
ORIGIN      
        1 ggggaaaaca aaagctttga cgattatttt tcagagacag ttacaaaaat cggcttaaaa
       61 ggtgaagagg cagaaattgc tttaatgagt caaaatgccg ttatgagtga tttacgcagc
      121 ttacgagatt ctatttctgg tgttaacatc gatgaagagt tagcagatat tattaaattt
      181 caacatggct acaatgccgc tgcaaagttt atttctgttg tggatgatat aatcaacacg
      241 gttataaatg ggcttcgtgc ataggcagat aggagatata tatgaggcga gtaagttctg
      301 gacaagcaaa tctagatgta caaaataatt tacgaagaca ggagcgaaat ttaaacaagg
      361 taaataatca actcggcagc caacagcgca tacaaagttt gcgcgatgat ccaattgccg
      421 caggccatct cgttcgctat cagtcctacg caaaacgaat tgagcaattc aaacacaatg
      481 ccgaaactat ttcagatcaa tttattctca gtgaaggcta tgttgaccat agcttacaaa
      541 taatgcatcg ccttcgagag ttggcagtaa ctggcgccca cggagtatac aataaagacg
      601 atttggcaaa catggcagca gaagttgatg aactattaaa agaaatcatt caaaacgcaa
      661 acgccctcga tgcagatggc aatgcacttt ttgccggaga gcgtagtaaa aatcaagcct
      721 tcattgtcga caaggggaat actacaggca gcgattccga gctcattcaa agagtccgtt
      781 acaacggttc cataaaacaa aatgaggttg aagttgatga acatgcctat atgtcagtaa
      841 atcgcagcgg caatcagatt ttttgggcag aaagccaaca gctcatcgca cagcgcgacg
      901 caacttcata taccgtttta gaagatgcag tagtttctat aaacggcaca gatatttctc
      961 taaaaacggg tgactctatt catggcatta ttgccaaaat aaataattct ggagccccgg
     1021 taaaagcaag tctcgaccct atgtcgaatg cgcttatgtt acatacaaca gattctcgac
     1081 aactttggct agaagatatc gacggaaatg cctttgaaga tttgggactt ataaaagata
     1141 agagccaaag acctccctac aatctaaatg attcggtctt aagttcagga ggttctgttt
     1201 tcgacacagt tattgccttg cgaaatgcca tgctcaacaa cgacagcgaa agcatcggtg
     1261 ggaaaatttt gggaagcctt gactccgcta tcgataatct cacttcttcc ttggctgaac
     1321 tcggctcaaa atacgagcgt gcgcttttaa atgttgcccg ttcagaaaaa tcgatattga
     1381 atgtaacaag tcaaatttcc cgtgagggtg atttggatat aacgaccgca ataacagata
     1441 tgaaaatgct cgactatgta aaaaatgcta ccttgagcac agcgggcaaa ttatataatt
     1501 caacgctttt aaattatatt agataaaaga aggaaatgta aatgagcgaa caaagtgaaa
     1561 ttttcgaaaa aaaacttttg attgaaaaag gcttactggg ttttgaaact attacaaact
     1621 atagtttaac acatgccgat ttagagccct ttatgtggct caaagcagaa ggtgaaaaaa
     1681 acctcgcttt tttagttgta gacccattca tattattttc tgattatgaa atagacgttg
     1741 acgataattc tctttctgaa attgatataa aaaatccttc agatgtggtt gttttgacaa
     1801 ttttaactgt ttcaaaaacg ggtgaagagc ccacagcaaa tttacaaggg cctgttatcg
     1861 taaataaaaa caataataaa gcaatacaag tagtgctctc ggatccacgc tggatgacaa
     1921 agcattctat aacaaatcaa ttggagacac gaggcgccta atgcttatct tatcacgaaa
     1981 aataaatgaa aaaataaaaa ttggtgaaga cattacactc tctataatcg aaattaaggc
     2041 tgaccaggta aaaataggta tagatgctcc caaagaagta aaggtgtttc gccaagaagt
     2101 ttttaatgcc atacaaaatg aaaacctcgc cgctgctact tctactgagt ctatagaaag
     2161 ccttagcaat ttatttgcaa aaaaagagtt ttagagcggt aaaaagttct actttgagga
     2221 cgctacaatt ctttttttag aactactttg ttttcttcag cttcactcgc tcgcagataa
     2281 aaaggtcctt cccagtccat aagggggggg ctttttgctt tgtacagctc tccagccatt
     2341 tctaagagat aaaagcttgc tgcagaaaca tgtatagaaa gatattgaat gttcaatgca
     2401 gcattttctg ccaagagtgc atcgctaagc attttcgcat cggggccaac acaaaaaatc
     2461 ttttcttcag ggtcaagttt ttttacaaga tcctttgccg atatatccat ctcatcgaaa
     2521 actttttttc cattacgata gaaagaagta taaaaacggt ttttttttgc atcgatggct
     2581 ggtaaaataa gtccttcgag tgttaaaaag ggctttgcat acataacaag actcgaaata
     2641 gcataaagcg ggatttgaaa agaaagactc aaggctttta aagctgcgaa agcaagccgt
     2701 aagccggtaa atgtcccagg acccaaactt actgcggtaa attccaaatc ctttgcttca
     2761 aggtctactt cttttaacac gaaatctata gaaggaagca gtttctcaga ttgtttcata
     2821 gcgagatcga gggataaaag tgcttttttg tcgccattac atgctaaaaa attgatggta
     2881 tttgttgcgc catctattgc aagtgctttc ataggatttc cccatatgtc cagttttcta
     2941 gatgaatgct gcgactatca tcttcgttta cttcgaggcg aacatagatc gtatgttctg
     3001 gaaaaacatc aaggattttt tcgctccatt cgataacaca aacaccatcg ccgtagagca
     3061 tttcttcaga accaaggttt agaaaatctt ctaccgagtc aagtctgtac acatcgaagt
     3121 gataaagcgg aagtcgacca tagtattcgg aaataagggt aaatgttggg ctcgtaacat
     3181 cttcgtctat gcccaagcca agtgcaatgc ctttagtaat cgttgttttt cccgctgcta
     3241 aaaaaccctg cagggcaatg atatcccctt tttgcagatg acttcctatt ttttctccta
     3301 tccgtattgt ttcctcagaa gagaatgatt gtaaaatcat ttaagatatc cttctgcatc
     3361 aaactgattg attttatgtt ttaaagcacg tactaccggt aaaagtggat aatcaatcgt
     3421 ctcgggtatc gtgatttcaa tgtttttatt tccagttggt gcagtttcta ttataaactg
     3481 aaagggaaat tcaatttgtc tttgaggtat ttgcactacc gctatagaag aaaattttct
     3541 tctatagtag atatgatttt cctctgaaac aatattttta atttctaata ttttcattgc
     3601 attatagcat aattgaattg tgcagcttcg tcaatcatgg gactttgcta gacatagtca
     3661 tatacttttt tatatatttt attccttgtt tctagagaaa tttttacaaa ctctatgtgt
     3721 aaaatcgtta gttttgtttg cgttgtagtt gcattaacaa tttttccaaa ggctttttct
     3781 atcgcttcag catccattct aaattcaata taaatatatt gtcctttgga gataggcata
     3841 tttgtttgca aactacagcc atttgctgaa atatccataa gcgtgccttc atgttttttt
     3901 ggaagtgcct cataagaaac ttttggattt ttacctaagc cacccgaact cacttttaca
     3961 gctgaaaaaa cacactgcgg tttgtaagga actctttttt gatttcttcg cacaagtact
     4021 tctaaattat ttgtgtgaga aataaccatt tcattttctc gtgtattttg atatcgcaca
     4081 attcgcgttt gaaaggtata agctataaga tttttagttt caaaactaaa gttaattttt
     4141 gataaaggtt ttggcttaat ttcgtctcca taaatatttt ttggaacttg tacaataagc
     4201 ccttcttttg tgtttttcaa aactatactt ggatgatgat ctaagttttc atcgctatac
     4261 atgagtcgtt gcccttcaag tatatttttc gtgctcgtta taaaaatagt attttgtggg
     4321 tcttgctcaa tttttgttcg aatagaataa agaatagctt tttttttctc atccgctgtc
     4381 gtgtgtataa tagttttaaa taaagcatct attttagctt gatttcgcat aaacataata
     4441 aagtttgggc atttattatc cttacacagt tcttgtaaaa agtttttttc ttcatttgtt
     4501 aaatgcaaga tttgtgttac ataattgata tcctgtacgg tcgttggtcg atttggatca
     4561 tttacttttt tatgaaaact actttggaaa attcgtatta ttatatttat aagaaaaaaa
     4621 gcaagcccaa ttccccccgc tataagaagg ggtatgtaaa taacgggaga gtaatttcga
     4681 gtaaatccaa tatcaagtaa aatcattcat gtctctcctt ttattagttg tttttacaaa
     4741 ataccgaaag tgtttctaag ctcggtgcta tctcatattc tgctaagtca gctacaagaa
     4801 ccttatcgtt atttgctaaa gcgttttcaa attcaattaa aatcgaggaa aaattattta
     4861 agtagtctaa aatcatttca tcgtttatct ttgttttatt aaaatagttg gggaaaagac
     4921 tgagcaagga aatagttttt gaaaggctgg aaaaaaaatc agcaaattct gtaattattt
     4981 ttgaaacttc tgtatcttta tttgcttgat ataaaactcc aatatccaga aattgattgg
     5041 aaagtgtttc aagggggagg gtgaaagtcg ccaaagcttc tctaatgtct gaaaggagaa
     5101 ttgtttctaa gtttaagtct gagatttcgc tgagcggttt ttccataaaa gctgctaagt
     5161 catttgggtg caaaatgaga tcatcaatac ctaagcctat taaagcagca tcattttctt
     5221 caaaactttt ttgaaatgcc cttaagacat cgccaagagt tttttcgttt tcgagttcta
     5281 tatctgtttt ttgcttattt atggtgattt gcattctact tccttttatt tattgttatt
     5341 atatgaattt gtaaagtttg ttaaggaatc tgactgcgat tgtaaactat ttaaagccga
     5401 ctccatattt ccatatttta tttttaattc ttgctctttt tttgctattt gtacttctaa
     5461 gcgatctatt tgtttttgtg agctcgtaat ttttgtatcg agggaagcat ttttcataga
     5521 taaaataccg ccacttgtta cgtaggcttg cagttgctta tcaattaaat aggctatgcc
     5581 agaatcgatg acgaggtctc catctgtatc gatgccaaaa aggtttttaa tttctcccat
     5641 cgactctttt aaggcggcat cgagtttttt ttcatctatt tccaaatagc ctcgcaactg
     5701 cgtgtttgaa agacttcctc ccgaacttgc cttcgaagcg atcccaattt gtgagagcat
     5761 agaaatagta ttattcaatt caatggggta tgagttagta ataattcttt gtaaactaga
     5821 ttttgtattt gatacggtat attctgttct aaaaagcccc agtctttcat gggcttcttt
     5881 attttcttcc gttgtcaggt attgcagttc ttcaacaata tcagggtttg cttgcgagag
     5941 aatgttcatt tctgcaatga gctggttata gtttcctaca aaggttataa gggcatcttt
     6001 tgccttttcc tcatcaggtt ttatttgaat acttgctgtt ttttcagtgg gattttctaa
     6061 atgcaaggtg atgtcaggga taatgtcgtc aatagtattt gtaggacgag tcatgggaat
     6121 accctcatag cgtatctgtg catcttgcgc tatagaaatg ggatttaccg gagcgtatcc
     6181 atcgattttg tttggattgc gtgatgtaaa ctcagaaagc gaaagttctt ttcccgtgtt
     6241 tttattttct atgataattg agctgagctt aggatagtct gaaagcgcaa ttcgtatctt
     6301 ttcttttcct tctgtagata tgaggcttcg agttccatct tccattttaa gatagacaaa
     6361 ggaatcagat acgacaggct ctcgttgctc tatttctttt tcggttaaag ctgtttctat
     6421 tttttcatta aaaatagtga catttttaaa ttcagcaaaa agctgctcgt taaacagggg
     6481 actgctttgt ggacttgtat cttttgttat atcaggaact gtttttgaat gtacggtaaa
     6541 ttctataagc gattgtggat cctcttttac ggatttgggg atttgaatct caaagccaga
     6601 ttcaggaggc aggagaatgg ttttttctat gatttttgct tcgttttgaa atccaggaat
     6661 tctttgtaat tcgttttggc ttgtcccaaa gttttcaagc tcagatggag cagcttcaat
     6721 caattcgtag ttcaaagcaa gatcgagcgc catgtcgcta aatcggagtc tgttttctgc
     6781 acctgtttgt aaagattcta cgaggaggga ttttgtgctg cttgtaacac ctataagaga
     6841 cgcttttatt aaaccgtttg aacgacgatt taccgctgca ataaactcat cgagcgttcc
     6901 acccttccaa ttaaagctta gagatttttc tcctacatgg aaactgtatt ggcctttttc
     6961 tatagtagcg tttttttcaa taggtttgga taaaaaccta tctgcttttg cgatttcatc
     7021 aacatcaaat ttaaatgttt ctaaggcggc atttcgtcct ggagttgccg ttattgcaaa
     7081 ctcttgacta gaggatgaaa gtttttcact aaaaggatta tcaaaggaaa ataaggaacg
     7141 agacgtttct cgtaaattgg acatatagag atttaaacgc tgccaagatt tttgctcaaa
     7201 tttatagccg tccatcttat cttgttcacg ctttaaaggt attctttcag cttctacaag
     7261 ggctgctatg agatcatttg ttttgtattt atcacttact ccaggtatat tgatatctgc
     7321 cattagcctt tattcacccg cttttggcta tatctcttcg tcaataagta atcctaatac
     7381 ttctttcagt cgaatttgaa gattttgaac atcagcggat ggaatctcct taattacttt
     7441 atcggtttcc gaatcgacta tcttgactac aacctttccc aaggcctcat ttactagaaa
     7501 ttgtatttta cggcctgcta tatttgttgt ttcatttata tggcgtattt gagcttcagt
     7561 tttttgaatc tctttagcca cgtttgcagc ttcggcattg attttttttg tatcctcagc
     7621 cctctctgtc ggtgcaagtt ttggttttat cgtgctgatt atggaacggc catccattgc
     7681 cgaattctgc ccaatgctca taatactcat agggcttcct ccatgaagtt gtttagcttt
     7741 agtgaaagcg ttaaggaggg gcgctcctcc ttaaaacttc accaatgcag ggtagggctc
     7801 caagccctac cacagatctt agttgttctt aagtacgaaa aaaaattccg tacttcgtcc
     7861 aaaaaaagat gacatccaga tatctttttt tgaaatttag ctaagcaact tgagtacgtt
     7921 ttgagattgt gagtttgctt gtacaagcat tgcagcacca gattgtgtta gaatttggtt
     7981 ttttgtatat tccacaattt cagatgccat atcggtatct cgaattcgtg attcagctgc
     8041 ttgcaagttt tctgctgcaa cagcaattcc ctttgctgca atttcaaaac gattttgata
     8101 ggctcctaga tctgctcttt gtttatttac agatttaagt gcttcatcaa ttgtcataat
     8161 agcggagttg gcagtttcag gtgaagaaat tgaaattcta tcatctgagc cttgtccagc
     8221 tactaaacca agcgcttgag ctgtcattgt gccaatatag attctttcat tttgatctgt
     8281 atttgcacca atatgaagtt gcatcactcg accagctgca gaatcaaatg caaatgctcc
     8341 tgtaagcatg ttcatgttgt taaactgagc atgattcgca attctatcaa cttctgctac
     8401 gagttgtgaa acttcaactt gaatttgcat tcggtcttca tcagaataaa taccgtttgc
     8461 agattgtatc gaaagttctc ttagacgttg caaaatatca gttgtttctt caagatagcc
     8521 ttctgttgtt tgaatgaagg aaacaccgtt ttgaatgttt ctttcagctt gattcaaacc
     8581 acgaatttgg cttctcattt tttcagatac agctaatcca gatgcatcgt ccccagcctt
     8641 gttaattttt aagccggagc taagtttttc tatgtttccc gcaatattgg cattatttgc
     8701 gcctaatgtg cggttggcat acatagaact catgttgtga ttaataatca tacataccct
     8761 ccatgt
//
LOCUS       DAOSSW010000073         8397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_8022_length_8397_cov_5.638216, whole genome shotgun sequence.
ACCESSION   DAOSSW010000073 DAOSSW010000000
VERSION     DAOSSW010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 8397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8397
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8022_length_8397_cov_5.638216"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..911)
                     /locus_tag="PKW26_06640"
     CDS             complement(99..911)
                     /locus_tag="PKW26_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOQ93279.1"
                     /translation="MKKVKMGFISLCLLMAIVSFFSCTKENANLNSLEKIKEKAVFVL
                     GLDDSFPPLGFRDDNNNIVGYDIDLASEVAKRLGVKLECQPIDWAAKEQELNTGKIDC
                     IWNGFTMTAEREEALSFTKPYLDNAQVVIVRSDSGYKTLSDLAGKTIGLQAGSSAAEA
                     VDSVPSFKKSLKNIVEFKDNLTAFMDLEIKGVDAVVMDMVVGNYSIQTTGKPFVVLEE
                     VLASEKYGIGFRKGDVELRDAVQDALLAMAKDGTIAEISNKWFGRDISVVGK"
     gene            complement(1032..1940)
                     /locus_tag="PKW26_06645"
     CDS             complement(1032..1940)
                     /locus_tag="PKW26_06645"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOQ93280.1"
                     /translation="MDFFEIQAFYELSKTLHFSKAASIIHLSPSALSRLVMRLEDELG
                     VQLLDRDTRQVQLTEDGKLFLRFAQDILHQKNMIENTFRERSETISGNLRIYASVTAC
                     YTILPEFIEGISKDYPDIKLLVETGDPALAFKMLEDDECDIAVSALPNKRDMQKTVLA
                     DFESFPIKTSPLVFVAKKDSAYAAFTKETIYKEAHFIIPKRGLARKRFDLATKQMQVH
                     IAAETAGNEAVLALTALGLGLGLVPEIVLDNGPFSQGLMAYPPEMSGLGAYSIGFVMK
                     KNIRETHASFRLLQVARSIIESFGEV"
     gene            2155..3408
                     /locus_tag="PKW26_06650"
     CDS             2155..3408
                     /locus_tag="PKW26_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ93281.1"
                     /translation="MFLLLVIGLLAISIILFVLTKIHRKKNNALEKADKAKTDFLARM
                     SHEIRTPLSAIIGLNTLALEKLVKVDGAEIVSTYLKKIDISSRHLLELVNNILDISKI
                     SEGQMKLKKTNFSINSLLSSLYDIYFIEAKNKNLTLSIQNAIKDFDTIIGDSLLLKEV
                     LSNLISNAIKFNKSQGIVTLSVEDRTSSTNDTHSENIKTIRFTINDSGVGMHRKMIEK
                     IFDPFERDEIWDVKQIRGTGLGLPLSKKIISLMGSNLHVKSTPGIGSTFWFDIEVPVA
                     TKAIKQEEKQRLVVNLKGKNILIAEDDEINAEILEQILISFSAEKITRAKNGKECVKA
                     FEKSEIGYYDYIIMDIRMPIMDGYTATKIIRQMDRADAKTIKIIALSANAFAEDKEKS
                     TSVGINYHCAKPLDNEDFMRALLAP"
     gene            3558..5036
                     /gene="ilvC"
                     /locus_tag="PKW26_06655"
     CDS             3558..5036
                     /gene="ilvC"
                     /locus_tag="PKW26_06655"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOQ93282.1"
                     /translation="MKKTNYFNAIPWREALMQLGHCRFMEKEEFSDGIKALAGKKIVI
                     LGCGAQGLNQGMNLRDSGLNVSYALRKEAIAEKRQSWKSATENGFKVGTIEELIPEAD
                     LVGNLTPDKQHTNALSGVVPLMKKNAALWYSHGFNIVEEGFQIRKDITVVMMAPKGPG
                     SEVRTEYVRGFGVPTLIAVHPENDPEGKGWSIAKALAVGCGGSRAGVLESSFVAEVKS
                     DLMGEQTILCGMLQTGSLLCFDKMVEKGLDKGYAAKLVQYGWETVTEALKWGGITGMM
                     DRLSNPAKIKAHELSLELKKIMTPLYQKHQDDIISGEFSKTMMEDWNNNDKNLLSWRE
                     ATGQTAFEQSPAGEIDIAEQAYFDNGILMVAMVKAGVELAFETMVDAGIKPESAYYES
                     LHETPLIANLISRKKLYEMNKVISDTAEYGCYLFDNACRPLLKDFMAKVDVDIIGKGL
                     EFENTAVSNAELVKVNAEIRNHPVEKIGQTLRSYMTSMKAIV"
     gene            5149..5952
                     /locus_tag="PKW26_06660"
     CDS             5149..5952
                     /locus_tag="PKW26_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ93283.1"
                     /translation="MKKFSIFTFDPFRLLGFSGFVALGISVLIDIFFGIHNPQTLIPF
                     VEITTFGINFFSMSLCLMIIIWPKQKKYTLLVLLIESIYTMAIGYEFISLTLYGLFLI
                     LLFTMDFYSQKLRLKGFISLVIWILLLLTLIPFGWNRFFLCFGLSFFTFSSYLCIYWV
                     LFQKLSIVPSDYQLHTINFKLPKVGSLLHLGQFPLSKRQIQCVYYLLNGFYTYKEISD
                     LCYISVSVIKKEMLDIYKVFGVQNREMLYFLLSQYTLCYPDDIKQKPRD"
     gene            5956..7053
                     /locus_tag="PKW26_06665"
     CDS             5956..7053
                     /locus_tag="PKW26_06665"
                     /inference="COORDINATES: protein motif:HMM:NF022635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HOQ93284.1"
                     /translation="MADILDYIDWRGDLSFESAQFNSVDNLIFSALSYINFSTYVSQD
                     FSNPISFRELLPAFKTKTKSQEQELGLFINKKTFDLFFKAAESKRYGGCLIQAHVQIL
                     DAESETQFSATSFTFGHKQHCIAYRGTDDSILGWKEDFNMSFMETIPSQIEALNYFEK
                     ACKALRGTFILVGHSKGGNLAIYTGLQCNVKDKKRIAKIYSNDAPGFLKKTIESKNFT
                     EIKERISSFIPEFSIIGLLLNHTIPSQIVESSQTGMMSHDPFSWQVRGPAFVPSGKLN
                     KKSSIIDTTLKNWLSSLDTRTREQFIDSFFEAFLATKVSTLTELSVNWLKHSATILKT
                     LHLADSKTKETIMKSLHSLLMSYIDVKLSKK"
     gene            7168..>8397
                     /locus_tag="PKW26_06670"
     CDS             7168..>8397
                     /locus_tag="PKW26_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOQ93285.1"
                     /translation="MNLYHKINHRIEETYKHKDEAFQIVARLLFATLLGLSCISLIVV
                     FIVEKQGLEVPILIVLMVVLIILSFLVLKGRAVFASLALSYLLPILLSTLIFTFPNTS
                     GQMELYVIGFMNLFSMTLTSFIGFYAWQTFPIIAINLVAVILNIVLRIIPYSLKNNIE
                     LQIDEAVIVSILTLFAAICFFMVQKRSTRIIQNIKKLNTIADNRLQVLQAAMQASTEV
                     LSTGDQLSQSAAKSTSLTENVTQSAVTVAFSMNKVLKDSERLSEELNGIAENSNTVKQ
                     STESQSSVINQTSAAIEEMTASIHNIAHITRERKTAVVSLAKSTEEGQNIVNSFSEEM
                     KKVEASTGSILDIVRVISSVASQTNLLAMNAAIEAAHAGEYGRGFAVVADEIRKLSEQ
                     TNKNVREITETVKLTISD"
BASE COUNT         2762 a         1600 c         1484 g         2551 t
ORIGIN      
        1 ataaatcttc ctagaattac aaaaaatagg ctccgcagtt tttcaatttt tctagagatt
       61 agactacgga gcaaacaatt gttttatatt tttacaaact atttcccaac gacagaaata
      121 tctctaccaa accatttgtt agaaatctct gcaatagttc catcttttgc catagcaagg
      181 agggcatctt gcacagcatc gcgcaattct acatcgccct ttctaaagcc tatgccgtat
      241 ttttctgaag caagcacttc ttcgagaaca acaaagggtt ttccagttgt ttgaatacta
      301 tagtttccaa caaccatatc catcaccaca gcatcaaccc cttttatttc aaggtccata
      361 aatgcggtaa gattgtcttt gaattctact atgtttttta gactcttttt gaaagaaggc
      421 acagagtcca cagcttctgc tgcacttgaa cctgcttgta gcccaattgt ttttccagca
      481 aggtctgata aggttttgta accggagtcg gagcgaacaa ttaccacttg tgcattgtcg
      541 agatagggtt ttgtaaaaga aagcgcttct tctcgctcag ctgtcatcgt aaagccattc
      601 caaatacagt cgatttttcc ggtgttgagc tcttgctctt ttgctgccca gtcgattggc
      661 tggcactcaa gttttacccc taaccgcttt gcaacttcgc tcgctaaatc aatgtcatag
      721 ccaacgatat tgttattatc atctcgaaaa ccaagcggtg gaaaagagtc gtcgagacca
      781 agtacaaaca cagccttctc ttttattttt tccaaagagt ttagatttgc gttttccttc
      841 gtgcaagaaa aaaaacttac aatcgccatc agcagacaga ggcttataaa gcccattttt
      901 acctttttca ttttaccatc ctttttttat tactttttag taagtataaa catttcaatt
      961 taattataca agaagccttt taccttccat aaaaacaaaa gaatcgatag cctaaaataa
     1021 aatcctacac ctcatacttc tccaaagctt tctattatac tacgggctac ttgtagaaga
     1081 cgaaaagaag catgcgtttc tcgtatgttt tttttcataa caaagccaat actatatgca
     1141 cctaagccgc tcatttcagg agggtatgcc ataagacctt gagaaaaagg accattatcc
     1201 aagactatct ctgggacaag tccaagtcca agcccaagag cagtcagcgc aagaacagcc
     1261 tcatttcctg ctgtttctgc tgcaatgtga acttgcattt gttttgtagc tagatcaaaa
     1321 cgttttcgag ctaatccacg ctttggaata ataaaatgag cttccttata aatagtttct
     1381 tttgtaaaag ctgcataggc agaatctttt tttgcaacaa agacaagcgg cgaggttttt
     1441 atgggaaagc tttcaaaatc cgcaagaact gttttttgca tatctctttt attgggcagg
     1501 gcagaaaccg ctatatcgca ttcgtcatct tcgagcattt taaaagcgag ggctggatct
     1561 cctgtttcaa ccaagagttt tatatctgga tagtcttttg atatcccttc tataaactca
     1621 ggcaaaatcg tataacaggc ggttacagaa gcatagatgc gcaaatttcc agaaattgtt
     1681 tcacttcgct cacgaaaggt attttcaatc atgttttttt gatgcaagat atcctgggca
     1741 aaacgaagaa aaagctttcc gtcctctgtt aactgtactt ggcgcgtatc gcgatctaaa
     1801 agttgtaccc ctagttcatc ttccaggcgc ataacaagac ggcttagtgc agaagggctt
     1861 aaatggatta ttgatgcagc cttcgaaaag tgcagtgttt tggataattc ataaaaagcc
     1921 tgtatttcaa aaaaatccat gcactaatta tatggtattc atgcataaaa cgcaagaatc
     1981 ttttttattg cgctatttaa aaaggcatgg tataatagcc taaaagtaat aggtatataa
     2041 gtgaaaaaat cgtttattag tatttttctg ttttttttat ttacttctct ttcgtggact
     2101 gtgcccgata cgaacttaca aagctatata aaaacaaacc ctttcgagtc ctccatgttt
     2161 ttattacttg taattggact tctagctatt tctattattc tttttgttct tacaaaaata
     2221 cacaggaaga aaaataatgc acttgagaaa gcagacaagg caaaaaccga ctttctcgct
     2281 cgaatgtcac atgaaatacg cacgccgctg agtgctataa ttggcttaaa tacccttgct
     2341 cttgaaaagc ttgtaaaggt tgacggtgcg gaaatagttt ctacatatct aaaaaaaata
     2401 gacatttctt ctcgtcatct tttagaactc gttaataata ttttggatat ttcaaaaatt
     2461 agtgaagggc aaatgaaatt aaaaaaaaca aatttctcaa tcaactcgct tttaagctct
     2521 ctttatgata tctattttat agaagcaaaa aataaaaacc taacactgag tatacaaaat
     2581 gctataaaag attttgacac cattatagga gatagcctac ttttaaaaga agtactttca
     2641 aatcttattt caaatgctat aaaatttaat aaatcacaag gtattgtaac actttctgta
     2701 gaagatagaa caagttccac gaacgatact cactcagaaa atataaaaac aattcgtttc
     2761 accataaatg actcaggtgt aggcatgcat cgtaaaatga ttgaaaaaat ctttgacccc
     2821 ttcgaacgag atgaaatctg ggatgtaaaa caaattcgtg gaaccgggtt gggattgcct
     2881 ctttcaaaaa aaattatttc ccttatggga agcaacttac atgttaaatc tacaccagga
     2941 ataggctcca cattttggtt tgatatagaa gtacctgtag cgacaaaggc tatcaagcaa
     3001 gaagaaaagc agaggcttgt tgttaacctt aaagggaaaa atatactcat tgccgaagat
     3061 gatgaaatca atgctgaaat tctagaacaa attctcatat ctttttctgc tgaaaaaatt
     3121 acaagagcca aaaatggcaa agaatgcgta aaggcttttg aaaaatctga aataggctac
     3181 tacgattata ttataatgga tattcgaatg cccataatgg acggttatac agcaacaaag
     3241 attattcgcc agatggatag agcagacgca aaaacaatta aaataattgc cttgtccgca
     3301 aacgcttttg cagaagataa agaaaaatca acttctgtcg gtatcaatta tcattgtgca
     3361 aaaccacttg ataatgaaga ttttatgaga gcgcttttag caccataact tttatactaa
     3421 acaattgctg cacaaagcgc aaaagtgcaa tgtataaatt acgctttaca caataattga
     3481 ttttatttac tataaccata ctgttattat gatagaatct atgaaaatgg taaagaaaaa
     3541 aacaggagga aatacatatg aaaaaaacaa attattttaa tgcaatccct tggcgcgaag
     3601 ccttaatgca gcttgggcat tgtcgattta tggaaaaaga agaattctct gatgggatta
     3661 aagcccttgc tggaaaaaaa atagttattc ttggttgcgg ggcacaaggc ttaaatcaag
     3721 gaatgaactt gcgcgactca ggcttaaacg taagctatgc gctcagaaaa gaagcaatcg
     3781 ctgaaaaaag acaaagctgg aaaagtgcaa ctgaaaacgg ttttaaagtg ggcacaattg
     3841 aagagctcat cccagaagct gaccttgttg gaaatcttac tcccgacaaa caacatacca
     3901 atgccctaag tggcgtcgta ccacttatga aaaaaaatgc ggccctttgg tatagccacg
     3961 gtttcaatat cgtcgaagaa ggctttcaaa tacgaaaaga cattacggtt gttatgatgg
     4021 caccgaaggg accaggttct gaagtgcgta ccgaatacgt tcgtggcttt ggcgttccta
     4081 ccctcatcgc cgttcatcca gaaaatgatc cagaaggaaa aggttggtct atagcaaagg
     4141 cactcgctgt cggctgtggt ggcagtcgtg caggcgtact tgaatcgagc tttgttgcag
     4201 aagtaaaatc cgaccttatg ggcgagcaaa ccattctttg cggtatgctt caaacaggct
     4261 cccttctctg cttcgataag atggtagaaa aaggcttaga taagggctat gccgcaaaac
     4321 tcgtgcaata tggttgggaa actgtcacag aagctctcaa atggggcggg ataacaggca
     4381 tgatggacag gctctcaaat ccagcaaaaa tcaaagcaca tgagctttcg cttgagctta
     4441 aaaaaattat gactcccctc tatcaaaaac atcaagatga cattatttct ggggaattta
     4501 gtaaaaccat gatggaagac tggaacaata atgacaaaaa tcttctttca tggcgagaag
     4561 ctaccgggca aacagccttt gaacaaagtc cagcaggaga aatagacata gctgaacaag
     4621 cttactttga caatggcatt ctcatggttg ctatggttaa ggcaggcgtt gaacttgctt
     4681 ttgaaacgat ggtagatgca ggaataaaac cagaaagtgc ctattatgag tcgcttcacg
     4741 aaacaccgct catcgcaaat cttataagcc gaaaaaaact ctacgaaatg aacaaggtta
     4801 tttctgacac cgctgagtat ggctgctatc tcttcgataa cgcctgccgc cccctcctaa
     4861 aagattttat ggcaaaggtt gacgtagaca taatcggaaa aggactagag ttcgaaaata
     4921 cagctgtttc aaatgcagaa ctcgtaaaag taaatgctga aattcgcaat cacccagtag
     4981 aaaaaatcgg tcaaacactt cgaagctata tgacttcaat gaaggctatt gtataaaagt
     5041 ctttttgtct taaacccaaa atcacaaaac acagaggttt tgggtttaaa agatagaaaa
     5101 aatagatttt tattgttttt ttttaaatct agtttatact aatagtatat gaaaaaattt
     5161 tctattttta cctttgatcc ttttagactc ctcggattca gtggcttcgt tgctttagga
     5221 atctcggtgc tgatcgatat ttttttcggt attcacaacc cacaaaccct catccccttt
     5281 gtggaaatta caacatttgg tataaatttt ttctccatga gtctctgcct tatgataatt
     5341 atttggccca agcaaaaaaa atacaccctt cttgtattgc tcatcgagtc aatatatacc
     5401 atggcaattg gctatgagtt tatttcactg acgctgtatg ggctttttct aattttattg
     5461 tttaccatgg atttttatag ccaaaaactc cgcctaaaag gctttatttc actcgttatt
     5521 tggattttat tattattgac ccttattcct tttggatgga atcgattttt tttatgtttt
     5581 gggctttctt tttttacctt ctcatcctat ctttgtattt attgggtctt gtttcaaaaa
     5641 ctttcgattg ttccctccga ctatcaacta catactatca attttaagct accaaaggta
     5701 ggaagccttc tacatcttgg acaattcccc ttgagtaaac ggcaaatcca atgcgtatat
     5761 tatttattga atggctttta tacatacaaa gaaatttccg atctttgcta tatttctgta
     5821 tctgttataa aaaaagagat gctcgatatt tacaaggttt ttggtgtgca aaacagagaa
     5881 atgctttatt ttctactgtc gcaatatacc ctatgctatc ctgacgatat aaagcaaaaa
     5941 cctcgggact aagctatggc agatatttta gattatatag attggcgcgg agacctcagt
     6001 tttgaaagtg cacagttcaa ttcagttgat aatcttatat tttcagcact ttcatatatc
     6061 aatttttcta cctatgtctc tcaagatttt tcaaacccta tttcatttag agaattgctc
     6121 cccgcattta aaacaaagac aaaaagtcaa gaacaagaac tcggtttgtt tattaataaa
     6181 aaaacctttg atttgttttt taaagcggca gaatcaaaac gttatggagg ctgtcttata
     6241 caagctcatg ttcaaatact cgatgcagaa agtgaaacac agttttctgc aacaagcttt
     6301 acttttggac ataaacaaca ctgcatcgca taccggggaa ctgatgatag cattcttgga
     6361 tggaaagagg acttcaatat gtcctttatg gaaacaatac cttcacaaat tgaagcgcta
     6421 aattattttg aaaaagcctg caaagcccta cgaggtacat ttatactcgt cggacattca
     6481 aaaggaggga atctggcaat ttatactggc ttacagtgca atgtaaaaga taaaaaacgg
     6541 atagcaaaaa tctattcaaa tgacgccccc ggatttctaa aaaaaacaat tgagagcaaa
     6601 aactttacag aaataaaaga acgcattagc tcatttatac ccgaattttc tatcatcggc
     6661 ttgttattga atcatacgat accgagtcaa attgttgaaa gcagtcaaac aggtatgatg
     6721 agtcacgatc ctttttcttg gcaagtacgc ggtcctgcct ttgtaccgag tggaaaatta
     6781 aacaaaaaaa gtagcatcat agacacaacg cttaaaaact ggctttcttc tctcgataca
     6841 aggacgaggg aacagtttat agacagcttt tttgaagctt ttttggcaac aaaggtctct
     6901 acgctcactg aactttcagt aaattggcta aaacattctg caacgatact caaaacctta
     6961 catctcgctg acagtaaaac aaaggaaacc attatgaaaa gcttacatag tctccttatg
     7021 tcctatatcg atgtaaagct tagtaaaaaa taatcgaaaa aaagagtttt tttagataat
     7081 cattttctct tgtgtaattt tttttatgtg tctttatcac caattttcct tatttacaat
     7141 ttccacaagc tgatttatac tgcgtgaatg aatctatacc acaaaataaa ccaccgaatc
     7201 gaagagacat ataagcacaa ggatgaagct tttcaaatcg ttgcaagact tctttttgca
     7261 accttgttgg gactttcatg tatctcttta atagtagttt ttattgtaga aaaacaagga
     7321 ctagaagtcc ccatacttat agttcttatg gttgttctca ttattttgtc tttccttgtt
     7381 ctaaaaggta gggctgtttt tgcaagcctt gctctaagtt accttctgcc tattttactt
     7441 agtaccctta tttttacttt tccaaatact tcaggccaaa tggaattata cgtgataggt
     7501 tttatgaatc ttttttccat gacgctcacg agttttatag gtttttatgc atggcaaact
     7561 tttcctataa ttgccataaa ccttgtagct gttattttaa acatcgtatt aagaattatt
     7621 ccatatagct tgaaaaataa tatagagcta caaattgatg aggctgtaat tgtttccata
     7681 ctaacgcttt ttgccgcaat ttgttttttt atggtacaaa agcgatctac acgtattata
     7741 caaaacataa aaaaattaaa taccatcgca gataatcgac tgcaagtatt gcaagcagct
     7801 atgcaagcct caaccgaggt actttctacc ggtgatcaac tcagtcaatc tgctgctaaa
     7861 tcaacaagcc tcaccgagaa tgttacacaa tcagcggtaa cagtggcttt ttctatgaat
     7921 aaggttctca aagacagtga acgattgtcg gaagagctca atggcattgc cgaaaattca
     7981 aatacggtaa agcaaagcac agagtcacaa agcagtgtta tcaatcaaac atcggcagca
     8041 attgaagaga tgaccgcatc tatacacaat attgcacata tcacaagaga aaggaaaact
     8101 gctgttgtaa gcctggcaaa aagcactgaa gaggggcaaa acattgtaaa cagtttctcc
     8161 gaagaaatga aaaaggttga agcatccaca ggttctatac tcgatattgt aagggttatc
     8221 agttctgttg catcgcaaac aaatctttta gcaatgaatg cggctataga agctgcccat
     8281 gctggcgaat acggtagagg ttttgctgtt gtcgcagatg aaatccgaaa actttcagag
     8341 caaacaaata aaaatgttcg agaaataaca gaaacggtta aattaacaat aagtgat
//
LOCUS       DAOSSW010000074         8250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_8207_length_8250_cov_8.608664, whole genome shotgun sequence.
ACCESSION   DAOSSW010000074 DAOSSW010000000
VERSION     DAOSSW010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8207_length_8250_cov_8.608664"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..2146
                     /gene="dxs"
                     /locus_tag="PKW26_06675"
     CDS             230..2146
                     /gene="dxs"
                     /locus_tag="PKW26_06675"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOQ93286.1"
                     /translation="MQSIHSPDDIKNLSYAELRDLAKEIRQEIIRVVGKNGGHLASNL
                     GVVELTLALHRVFDSPKDAIVWDVGHQSYTHKILTGRLKDFDSLRMPGGLSGFTKPSE
                     SSHDYFNVGHSSTSISSALGLLVGRKTLSVDGKVIALIGDGALTGGMALEALSHAGQI
                     AKNLVVVLNDNQMSIDKNTGSLSRYLSRLTMTSHYQSFRNHFDAMIEKIPLLRSWLPS
                     FIFRTKRAIKALVFSNNLFSDLGFEYVGPLNGHSIPELERVFSRVKHLKKPVVVHVLT
                     QKGRGYSPAESDPTCFHGLGPFCIEDGSQETNTGKTFTQAFSESLLRLAEENPKIVAI
                     TAAMMGGTGLTEFAKRYPDRFFDVGIAEQHAVTFAGGLSVAGLKPVVAIYSTFMQRAL
                     DQLIQDISIQKIPAIFALDRSGPVPDDGETHQGIFDISLMRPIPNLSLLAPASAKELD
                     LMMTWAQLQNESVIIRYPKAVSPEEIPAFGKSLEPGRGVFISYEEILLKERKKTSQKE
                     NLFVTTGGMIPEVFEAVSLLSNEGIECDVYNLRFLKPLDKTYFIDQMKSYNQILFVED
                     GIRIGGIGTYLESLLQRNYIDKKTAVCGFPNRYFAQGKRKDILEDAHLSPRYLAKKML
                     RLFEVQGENIDASK"
     gene            2133..2948
                     /gene="folP"
                     /locus_tag="PKW26_06680"
     CDS             2133..2948
                     /gene="folP"
                     /locus_tag="PKW26_06680"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOQ93287.1"
                     /translation="MLQNDFAVRVMAIINLTPDSFWSGSRSLSKNHAVKKALQAVEDG
                     AHILDLGAESSRPGASYVSEKEELDRLIPVIQAIRKKTSIPISVDTRRSLVLKAAVEA
                     GASMLNDISALEDDPEMAKLVAKLQIPLVLMHKRGEPKNMQENTAYADVFEEVFLYLQ
                     GRVRYALDAGIAAENIIVDPGIGFGKNLEGNRVLIQRAGDFAAVIPGKKTEVLIGASR
                     KTSLGILTGRPVEERLVASVCAHQLAVQAGATILRVHDVKETIDMLGVLRGFG"
     gene            2945..>3570
                     /locus_tag="PKW26_06685"
     CDS             2945..>3570
                     /locus_tag="PKW26_06685"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOQ93288.1"
                     /translation="MILFEKLAVIYNIARPVLDIAFLTFIIYKAYGLIIKTQGAEILK
                     ALIMTAIAYGLALFLNLQTVLWIFNMLAPGFLIAFAIVFQPELRKIFLHLGQTAWLKP
                     GDRYKKGYVEGIIIAAEVLSEQKRGMLCVLERSISLREIIDGGTKLNADISSGLIVSI
                     FGSDTPLHDGAIVLQSGKIVAAGCFLPLSERQDIKKSFGTRHRAALGLA"
     assembly_gap    3571..3670
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3671..4467
                     /locus_tag="PKW26_06690"
     CDS             <3671..4467
                     /locus_tag="PKW26_06690"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOQ93289.1"
                     /translation="TSLPTHFVRVTVRGKPEDIVQISEQDFSAFLDLNAYVEAGSFSI
                     PVSLQVSKQATLFSPLEFSCKPERVTLNLDKKMLSYIPVSPSFTGDLQKGYELESFKI
                     EPAYIEALGPEKIILAIKEFKTKEVSLENASASFVQSVDIINTNNFVSFDPNINFSVH
                     VAIKKIFTTKDFRANLGYFSGLSENLFVEDAFPSYSLSLRGFINDIEDFSLDSHSLYV
                     DLSEIDEAGVYELPLEFNLPTDFEVLEFEPQSIVLELKEKKLAIED"
     gene            4475..4876
                     /locus_tag="PKW26_06695"
     CDS             4475..4876
                     /locus_tag="PKW26_06695"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOQ93290.1"
                     /translation="MILGVGVDIAKNKRFASWLRNEKKILRFFHKKEIDFSKITVQML
                     ASRFAAKEAFVKALGIGFHNIELKSIAVLKNAKGQPYFYLEGEAKKAFERLGGEKVHL
                     SLSHEKEYAIAFVVIEARGIENSLEEGWNEK"
     gene            4861..5775
                     /locus_tag="PKW26_06700"
     CDS             4861..5775
                     /locus_tag="PKW26_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HOQ93291.1"
                     /translation="MERKIKKKEKQVSVTFFSKQKVSCPVCKKDFEKEELMSGGGRLI
                     AGPLTDELRRTYEPSVKYGTLYPLTYAIGACPSCHIGLLWEDFDIKLDDKSINLILDN
                     EGERIESVQAIFPHYNFGRKRTLLDGAAAYYLALLTYEKLPASISPTIKKAQISLRLA
                     WVCTDLHTELPNRNYDYISQLFYRKALFFYQESLLYEMNNTEEITGIKSFGPDIDKNY
                     GYDGVIYLSGLLELKYGQTEDPILRLKKLDEYKRAIARVFGLGKSSKEKPGPLLELSR
                     ELYDTFSAILKDASNVDFTPSDDEGDDE"
     gene            5782..6555
                     /gene="truA"
                     /locus_tag="PKW26_06705"
     CDS             5782..6555
                     /gene="truA"
                     /locus_tag="PKW26_06705"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOQ93292.1"
                     /translation="MRTILLYLSYDGRNFCGWQSQRKINEERTVQGELEKALLKVHKT
                     KVLCQGSGRTDSGVHARRQAVSFDSPLRSIPVEKYPMALNAFLPIDIRVQSAHLMPSG
                     FSARFNATSRSYRYFLSTSQSPSAFSMPYSWALYRKPNIILLNAMASLLQGEMDCASF
                     ASSGDKSLSTKRYIYTASFFMEDEMLVFEISANAFLWKMIRSLVGTLIDFEKRYGDNA
                     VDEFQKLIDLKDRKRAGQTAPPQGLFMWDVSFEGERVHP"
     gene            6602..>8250
                     /locus_tag="PKW26_06710"
     CDS             6602..>8250
                     /locus_tag="PKW26_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93293.1"
                     /translation="MKKIFIGFAHRRLVFCYLLFLFFTLSSFAADESSSNEFEKNKSN
                     KKQVIVTEYFDIIFTEDSKQSALILADSVDALYLKACALLESEAYFRLPLVLVSGVDV
                     LNAYFSYVPYNHIVLYDTIPEEGSLAVFSESLLSVFYHEVVHAVSIQKKSKTLRNFSK
                     IFGDVFPPAPFLYTPLFMIEGVTVSLESRDGEGRLNDSFATQIIRQAKIEEKFPTWQQ
                     ASQARDIYPVGSTPYLFGGAFSDYLQNTYGMDSYGEFWGELGKFHFFGSLESIVAKVY
                     GKKLKELWNEFRDSISLPEPHSIIDPKNTNEIKSLQTKKALYRFLTQSQKGFAWFDAS
                     SNEIWYESFETGNRKKLFTTSNGQQSLSLSLDGNYLVSSFVEAYPFGNNKTVVYNLAN
                     KKKLQTFNSLRDASLVQLADGKDYLAGVKTEGQLASIEVWSFLEKKDKPAHLKNFPLN
                     SIPFSLQALDDGRLAYILKAKESWSLVFWNPLDGSEESIQVLSGREPLKYLNPVFFDD
                     NLIGLSFSYAEKDSLPKMGFFDFASKSIFLQSQNFSGGLHYP"
BASE COUNT         2457 a         1483 c         1726 g         2484 t
ORIGIN      
        1 caatgccttg tggaaaaaag cgaaaacgca aaaaaatagc aactcataaa cgaaaaaaga
       61 agcttagaag aaatcgacat aaaaataaat aagaccatat agggcagcag tcttttactg
      121 tggcttttat ttagatattc taaggattat cgtgcgagaa gagacctcag gttgtttaaa
      181 gcaagccggg gtcttttttt attaaggtgc gaaggagcga gctacgaaaa ttcagtctat
      241 ccattctcca gatgatataa aaaatctctc ctatgccgaa ttgcgagacc tcgcaaaaga
      301 aatccgccaa gaaattattc gcgtggttgg aaaaaacggc gggcatcttg caagtaatct
      361 cggtgttgta gagttgacgc ttgccttgca tcgggttttt gacagcccga aagatgccat
      421 tgtttgggat gttgggcatc agtcctatac gcataaaata ctgacgggac ggctaaagga
      481 ttttgacagt ttacgcatgc ccggtggctt gtctggcttt acaaaaccta gcgaaagctc
      541 tcatgattat tttaatgttg ggcattcttc aacttctatt tccagtgcct tggggctttt
      601 ggtaggtaga aaaacgctct cggttgacgg caaggtaatt gcgcttatcg gtgatggcgc
      661 tttgacaggg ggaatggcct tggaagcgct ctctcacgca gggcaaatag ctaaaaacct
      721 cgtcgttgtg ttaaacgata accaaatgtc aatcgataaa aacacaggaa gtctctctcg
      781 ttatttaagt cgacttacaa tgacgagcca ctatcaaagc tttcgaaatc attttgatgc
      841 aatgatagaa aaaataccgc tcttgcgttc atggcttccc agttttattt ttcgcactaa
      901 gcgcgccata aaggctcttg tcttttcaaa taatttattt tcagatcttg gttttgagta
      961 tgtcggtccg ctcaatgggc atagcatacc ggagctcgag cgtgtttttt ctcgcgtaaa
     1021 gcatcttaaa aaaccggtgg ttgtacatgt gcttactcaa aaagggcgag gctatagtcc
     1081 tgcagaaagt gatcctactt gttttcatgg actgggaccc ttttgtattg aagatggaag
     1141 ccaggagaca aacaccggga aaacctttac tcaagctttt tctgaatcgc ttttgcggct
     1201 tgctgaagaa aatcccaaaa tcgtcgcaat tactgcggct atgatgggag ggacgggttt
     1261 aacagaattt gcaaagcgtt atcccgacag gtttttcgat gtaggcattg cagagcaaca
     1321 tgccgtaacc tttgcaggtg gtttatctgt tgcagggctt aaacccgttg ttgctattta
     1381 ttcgaccttt atgcaaagag ccctcgacca attgatacag gatatttcca tacaaaaaat
     1441 tccggcaatt tttgcacttg accgctctgg ccccgttcct gatgacgggg aaacgcacca
     1501 aggtattttt gatatttcgc ttatgcgacc tataccaaat ctttcgcttt tggcccctgc
     1561 ttcagcgaag gaacttgatt tgatgatgac ctgggcacag ctgcaaaatg aatctgttat
     1621 tattcgctat ccgaaggctg tgagcccaga agaaatacct gcttttggga aaagcttaga
     1681 acctggtcgc ggtgttttta tttcctatga agaaattctc ttgaaagaga gaaaaaaaac
     1741 gagtcaaaaa gaaaatctct ttgtaacaac agggggtatg attccagaag tatttgaagc
     1801 ggtttctctt ttgtcgaatg aaggtataga atgcgatgta tataatctgc ggtttttaaa
     1861 gccgcttgat aagacctatt ttattgacca aatgaaaagc tacaatcaga ttctttttgt
     1921 agaagatggc atacgcattg ggggaatcgg aacctatctt gaatcgcttt tgcaaagaaa
     1981 ctatatcgat aaaaaaacag ctgtctgtgg ttttccaaat cgctattttg cacaagggaa
     2041 acgcaaggat attttggaag atgcacattt aagtcctcgc tatttggcaa aaaagatgtt
     2101 gcgacttttt gaagtgcagg gggaaaacat cgatgcttca aaatgatttc gctgtacggg
     2161 ttatggcaat catcaatctc acgcccgatt cgttttggtc gggaagtcgt agtctttcca
     2221 aaaatcatgc ggtaaaaaaa gccttgcaag cagttgaaga tggtgcgcat attttggact
     2281 tgggtgctga atctagccgc ccgggagcaa gctacgtgag tgaaaaagag gaactcgatc
     2341 gtcttattcc ggttattcaa gcaattcgaa aaaaaacctc tattcctatc tctgttgata
     2401 cgcgtcgctc ccttgtccta aaagctgctg ttgaagctgg cgcatctatg ctcaatgata
     2461 tttctgcgct tgaggatgat cccgagatgg caaaacttgt tgcaaaattg cagataccgc
     2521 ttgtgctcat gcacaaaaga ggggagccaa aaaacatgca ggaaaacacc gcatatgccg
     2581 atgtatttga agaggtgttt ttgtatttgc aaggtcgagt gcgctatgcg cttgacgctg
     2641 gaattgcagc agaaaatatt attgtggacc cagggattgg ctttgggaaa aacctagaag
     2701 gaaaccgagt cctcatacag agggcaggag actttgccgc agtcatacca gggaaaaaaa
     2761 ctgaagtgct tataggtgcg tcaagaaaga caagcctagg gattttgaca gggcggcctg
     2821 ttgaagagcg tctcgtcgct tctgtttgtg cgcatcaatt ggcggtgcaa gcaggggcaa
     2881 ctattttgcg agtacatgat gtaaaagaaa cgatcgatat gctaggggta ttacggggct
     2941 ttggatgatt ttatttgaaa aactggcagt tatttataat atcgcacgac ctgttttaga
     3001 tatagctttt ttaaccttta tcatttataa ggcttacgga cttattataa aaacacaggg
     3061 agctgaaata ttaaaagcgc tcattatgac agctatagct tacggactcg cattgttttt
     3121 aaatttacaa acagttttgt ggattttcaa catgcttgca ccaggttttt taattgcatt
     3181 tgcaattgtc tttcaaccag agttgcggaa aatatttttg caccttgggc aaacagcttg
     3241 gcttaaaccc ggcgatagat ataaaaaagg ctatgtcgag ggcattatca ttgcagcaga
     3301 agttttatca gaacaaaaac gtggtatgct ttgtgttttg gagcgaagta ttagtttgcg
     3361 agaaattata gacggtggca caaaactcaa tgcagacatc agctctggtt taattgtttc
     3421 tattttcggg agtgacacac ccttgcacga tggagcgatt gttttgcagt caggaaaaat
     3481 tgtagcagct ggttgttttc tgcctctttc ggagcgacaa gatataaaaa aatcttttgg
     3541 tacacgccat agggcagctc tcggcttagc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn taacgagcct gccaactcat tttgtacgag ttacggtaag ggggaaaccc
     3721 gaagatattg ttcaaataag tgaacaagat ttttctgcgt ttttggattt aaatgcttat
     3781 gtagaggctg gttctttttc tatccccgtg tccttacagg tttcaaaaca agctacttta
     3841 ttttcaccct tagagtttag ctgcaaacca gaacgtgtaa cattgaatct cgataaaaaa
     3901 atgctttctt atattccagt cagcccctct tttacagggg atttgcaaaa aggatatgag
     3961 cttgaatctt ttaaaataga gccagcctat atagaagctt taggtccaga aaaaattatt
     4021 ttagcaataa aagaatttaa aacaaaagaa gtttctcttg aaaatgcttc tgcctctttt
     4081 gtccaatcgg tagatataat aaatacaaat aattttgtaa gcttcgatcc aaatatcaat
     4141 ttttctgtgc atgtcgctat aaaaaagatt ttcactacaa aagattttcg ggcgaatctc
     4201 ggatattttt ctggactttc tgaaaatctt tttgttgaag atgcctttcc aagctactca
     4261 ttatcgttac gtggatttat aaatgacata gaagattttt ctctagattc tcattcactg
     4321 tatgttgacc tttctgaaat tgatgaggcg ggggtatatg agttaccctt ggagtttaat
     4381 cttccaacag attttgaagt tttggaattt gagccgcaaa gcattgtgct tgaattaaaa
     4441 gaaaaaaaac ttgctattga ggactaagct tggtatgatt ttaggtgtcg gtgttgatat
     4501 agcaaaaaat aagcgttttg cttcttggct tagaaatgaa aaaaaaattt tacgcttttt
     4561 tcataaaaag gaaattgatt tttcaaagat aacagtacaa atgcttgcct cgcgttttgc
     4621 agcaaaagaa gcttttgtaa aagcattggg gattggcttt cacaatattg aactaaaatc
     4681 gatagcggtg ttgaaaaacg caaaagggca accctatttt tatttggagg gagaggcaaa
     4741 aaaagctttc gagcgattgg gtggcgaaaa agttcattta agtttgagcc atgaaaaaga
     4801 atacgctata gcttttgttg ttatagaagc acgaggaata gaaaattctt tggaggaagg
     4861 atggaacgaa aaataaagaa aaaagaaaag caagtgtctg ttactttttt ttctaagcaa
     4921 aaagtctctt gtcctgtttg taaaaaagat tttgaaaaag aagaacttat gtctggcgga
     4981 gggagactta tagcaggacc acttacagat gaacttcgta gaacctatga accttccgta
     5041 aagtatggaa cgctctatcc acttacctat gctattggcg cctgcccttc ttgtcatatt
     5101 ggtttacttt gggaagactt tgatattaaa ctcgatgaca aaagtatcaa tttaattctt
     5161 gataacgaag gagagcgaat agaatcggta caagctattt ttccgcacta taattttggg
     5221 agaaaaagaa ctttgttaga tggggctgca gcctattatc ttgccttatt gacctatgaa
     5281 aaactgcccg cttctatttc tcctactatt aaaaaagcac aaatttcttt gcgccttgct
     5341 tgggtatgta ccgacttaca tacagaattg cccaatagaa attatgatta tataagccaa
     5401 ttattttatc gcaaagcatt gtttttttat caagaatctt tattgtatga aatgaataac
     5461 acagaagaaa ttactgggat aaaaagcttc ggcccagata tagataaaaa ctatggctac
     5521 gatggtgtta tttatttgag tggcttgctt gaattaaaat atggacaaac agaggatccg
     5581 attttacgct taaaaaaact tgacgaatac aagcgcgcta ttgcccgtgt ttttggtttg
     5641 ggaaaatctt ccaaagaaaa accaggtccc cttttggagc tttctcgtga gctctatgat
     5701 accttctctg ctattttgaa agatgcgtcg aacgtagatt ttacaccaag cgatgatgaa
     5761 ggtgatgatg aataaaattt tatgagaact attttattat atctttccta tgacggtaga
     5821 aatttttgtg gctggcaaag tcaaagaaaa ataaatgaag agcgaacggt gcaaggagaa
     5881 cttgaaaaag cacttttaaa ggtacataaa acgaaggtgc tttgccaagg atctgggaga
     5941 acagattctg gtgttcatgc aagacgacag gcggtcagtt ttgactctcc ccttcgctct
     6001 atacctgtgg aaaaataccc aatggctttg aatgcttttt tacccataga tattcgcgtc
     6061 cagtctgcgc atctaatgcc gagtggattt tcagcacgtt tcaatgcaac gagtcgcagc
     6121 tatcgctatt ttctatctac ttcgcaatcc ccctcagctt tttctatgcc ctatagctgg
     6181 gcattatacc gaaaaccgaa tattatactt ttaaatgcta tggcaagtct cttgcaggga
     6241 gaaatggatt gtgcttcttt tgcatcgagt ggtgacaaaa gtcttagcac aaagcgttat
     6301 atatatacgg catctttttt tatggaagat gaaatgctcg tttttgaaat ctctgcaaac
     6361 gcttttttgt ggaagatgat acgaagcctt gtcggcaccc tcatcgattt tgaaaaacgc
     6421 tatggcgaca atgccgtcga tgagtttcaa aaattgatag acttgaagga tagaaaaaga
     6481 gcagggcaaa ctgctccacc tcaaggttta tttatgtggg acgtctcttt tgaaggcgag
     6541 agagttcatc cataaaatat aaaaaaactg ccaataaaaa cacagaataa aaggattttt
     6601 tatgaaaaaa atatttatcg gcttcgccca tcgcagactt gttttttgct atctgctttt
     6661 tctctttttt actttgtcct cttttgcagc tgatgaatca agtagcaatg aatttgaaaa
     6721 aaataaatca aataaaaaac aagtgatagt tactgagtac tttgatatta tttttactga
     6781 ggattctaaa cagagcgcgc ttatccttgc tgactctgtc gatgccttgt acctaaaggc
     6841 ttgtgctctc ttagaaagtg aagcctactt ccgccttccc ctcgttcttg tttctggagt
     6901 cgatgtgctc aacgcctatt tttcctatgt tccatataat catattgttt tatacgatac
     6961 tatccctgaa gaaggtagct tggcagtttt tagtgaaagc ttgttgtcgg ttttttatca
     7021 tgaggtggtg catgccgtaa gcatacaaaa aaaatctaaa actttgagaa atttttcaaa
     7081 aattttcgga gatgtttttc caccagcgcc ttttctatac acgcctcttt ttatgataga
     7141 aggagtaact gtttcccttg aaagtcgaga cggcgagggg aggttgaacg attcctttgc
     7201 cacgcaaatt attcgtcaag caaaaataga agagaagttt ccgacctggc aacaggcgag
     7261 ccaagcacga gacatctatc ctgttggttc gacaccctat ctttttgggg gagctttttc
     7321 tgactatttg caaaatacct acggcatgga tagctatgga gagttttggg gagagcttgg
     7381 aaaattccat tttttcggat cccttgaatc aattgtcgca aaagtatatg gaaaaaaact
     7441 caaagaactt tggaatgaat tcagagattc tatttctctg cctgaacctc atagcattat
     7501 cgatccaaaa aatacaaatg aaataaaaag cctgcaaaca aaaaaagctt tgtatcgatt
     7561 tttaacacaa tctcaaaaag gctttgcatg gtttgatgct tcgagtaatg aaatctggta
     7621 tgaatctttt gaaacaggga atcgaaaaaa actttttaca acaagcaacg ggcaacaaag
     7681 tctatcactt tctctcgatg gaaattatct cgtctcttct tttgttgaag cctatccctt
     7741 tggaaacaat aaaactgtcg tgtacaatct tgcaaataag aaaaaactgc agacttttaa
     7801 ctcgctacgc gatgcaagtc ttgtacaact tgcggatgga aaagattatc ttgctggcgt
     7861 caaaactgaa ggacaacttg cgagtatcga agtttggtct tttttagaaa aaaaagataa
     7921 gccagcccat ctaaaaaact tcccgcttaa ttctattcct ttttctttgc aagcactcga
     7981 tgatggacgg cttgcctata tcttaaaagc aaaagaaagc tggtccttgg ttttttggaa
     8041 tccactagat ggaagcgaag agtcgattca ggttttatca ggcagagagc cattgaaata
     8101 cttaaatcct gttttttttg atgacaatct catcggtctt agttttagct atgcagaaaa
     8161 agacagcctt cccaagatgg gcttttttga ttttgcttca aaaagtattt ttttacaaag
     8221 tcaaaatttt tctggtggct tgcattatcc
//
LOCUS       DAOSSW010000075         8222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_8242_length_8222_cov_8.120975, whole genome shotgun sequence.
ACCESSION   DAOSSW010000075 DAOSSW010000000
VERSION     DAOSSW010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8242_length_8222_cov_8.120975"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PKW26_06715"
     CDS             complement(<1..294)
                     /locus_tag="PKW26_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93294.1"
                     /translation="MNFEKLKQGGIDVDDALVRFSGNRELLEKYLVKFLDDPVYKSIV
                     LNMDEENWGTAEDSVHSFKGITGSLAITALFETSCALLEALRVQDYELAIELYE"
     gene            454..1491
                     /locus_tag="PKW26_06720"
     CDS             454..1491
                     /locus_tag="PKW26_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOQ93295.1"
                     /translation="MLAKKRIFALVCAVLILFVSCTKNEKKTDSLSIAIFVPGVVAGN
                     AVYEMFVSGSEKAIESYKTKESAKSLKYQVIEAGTNQSEWPAKLTALCASEQWDLIFS
                     SNPALPDILEPILKDFPNQKCIILDSYKTGIAGMQTVLYNQRQQAYLAGYAAAMVTTA
                     KDLPYANSAKKIALIAAQEYPAMNDIMYPGFIEGARAYDPEIEVLFRIVGNWFDASKS
                     YEIASSLYDLGVDVILPIAGGASQGVISCAKDKSFYISWFDNEGYEKAPGYVISSAMM
                     MQEKMAEQVLGNYLDGRAEFGTANIYGMEDGFIDFVLHEPTSDNPERVQKIAEVYDAI
                     KTKRLVLEPSL"
     gene            1504..3030
                     /locus_tag="PKW26_06725"
     CDS             1504..3030
                     /locus_tag="PKW26_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ93296.1"
                     /translation="MFYLRATNISKKFTHKKAVDGSFLSVFSGQIHALLGENGAGKST
                     LAAILSGVIQASEGEIEIGYSPFRTESLKLKPAQIQSPKDAQERGIVLVHQRPILSES
                     LSVLENTVLGDLHRRIFFSKKKLKKTLNKILKDWEIELDLNKKGSQLNAEESFFAELL
                     SALYKNPKLLILDEPTALLPEKKRKAFFMSLKTQAEKGLAVLLITHNLQEALDNANFI
                     TVLKKGRVVLAKQNSQNKRIQMSALENALFTSDINLKQEKIQNKKNAEKNISLQKTHT
                     KGQIKKAQKDEAKKIFALSKVFYTGKKYPSLFNISFSVYEGAICYIYGQKASGIESLE
                     EIITGIQKMPFQGTIFFNEDNFTKQLSPRILRKGGAAIISTNKNYRASNPSLSIVELL
                     SVYKAVDEKAEDAETFSKNLIQLEKMPIEIHEPVSNLSGGMLQRLILERELSTEPNLL
                     LFFEPAQGLDAQRLASLANHLRALADMGKAILLVSANREKEFSAIADIQYEMRGGSLV
                     "
     gene            3027..4073
                     /locus_tag="PKW26_06730"
     CDS             3027..4073
                     /locus_tag="PKW26_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ93297.1"
                     /translation="MKKALSKIFCTIPRSQKKFPYTKALASLALGFLAAGILVFYFSS
                     YSFAAVIDFFLSPFSSVFFFGSMLASMSLLLVGSLGSSVALLSGNYNLGGEGQVYLGG
                     LITALILSKLDIFFAPLLAIILVIIASALLSFIPIILKLYRGASELLTSFLLSAALIP
                     LIDWAIAIPLRNKGQNLLATSPILARFEMTKILPPSSLSIVFFLSILLVFIAWRILYR
                     TKEGELFRISGKAEEFSFYAGFKTKRYNIIAMSISGALHGLVGYIAVVSIHHTCHSGF
                     YLGLGWNALSVALIAKSYPLLLIPASFILAYLFSASDYAVLFYSMPFNASFLIQGIVL
                     FAIAASHIGGKKNG"
     gene            4066..4902
                     /locus_tag="PKW26_06735"
     CDS             4066..4902
                     /locus_tag="PKW26_06735"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ93298.1"
                     /translation="MDNIFTILILSSPLLLASFGALVSENAGVMAVFIDGIISLSAFL
                     FFSFTVLFQNPVYAAILTLFLCSILLLASSFYATKTKANPFLVGLAINLLSTGLIALF
                     SSIFFHTQGVLTNHAFFNSYTSNLSRSWGTVLSFALVLFFSLFYKKTKPGLALQITGL
                     ESEVLYQQGLKPRFFRHFSWIIASLFASLSGILLVLRLSSFVPNISAGRGWIALAIVF
                     FGRKKPLGVLLAVLVFASAEWASVFLQNTGMLENVSSGIFIALPYLVSLILFALLPQK
                     KQ"
     gene            complement(4980..6263)
                     /gene="eno"
                     /locus_tag="PKW26_06740"
     CDS             complement(4980..6263)
                     /gene="eno"
                     /locus_tag="PKW26_06740"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOQ93299.1"
                     /translation="MNNVVITKVIGREIIDSRGNPTVEAEVHLCDGTIAFGAAPSGAS
                     TGIFEALELRDADKKRYGGKGVTKAVAAINTTINDLLVGKNPYDTYAIDRAMIEADGT
                     KDKSKFGANAILAVSIAAARASAQSLGIPLFRFLGGVNATMLPVPMMNILNGGAHAAN
                     TVDVQEFMIMPKGASSFAEGLRWCTEVYHALAGTLKEKGLLGGIGDEGGFAPNLSSDA
                     EVLDIILESIKKAGYKAGSDFVLALDAAASEWKAGKVGEYKMPKSGRTYTAKELVAHW
                     KDLVEKYPIFSIEDALDEEDWEGWNLLTQELGDKVQLVGDDLFVTNTERLSRGIAEKS
                     ANSILIKLNQIGTVSETLEAIKMAHNAGFSAVISHRSGETEDTTIADLAVALNAGQIK
                     TGAPARSERVAKYNQLLRIEEFLGENAQYKKLKSY"
     gene            complement(6272..>8222)
                     /locus_tag="PKW26_06745"
     CDS             complement(6272..>8222)
                     /locus_tag="PKW26_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOQ93300.1"
                     /translation="VIEMFFLSGENFPRIWKRLLFLFVLLSLSFVLVAGDGSLTILFT
                     HDMHDHLTPSNTLQNGQSMSTYGLTRIASLVKEERKKDSELLLVDAGDYSMGTLFQTI
                     FSTHSPQLRIMGKIGFEATTLGNHEFDFRASGLAKSLNTAKASGDKLPELLVGNVTFP
                     KNAAGNLTPSLAELEAALANYPAKEYILLEKKGYTIALFAVMGKDSASNAPMAEVTFT
                     DYIENARRIVSDIQANENVDMILCLSHSGTEGKSPNTEDEILAKKVPEIDVIISGHSH
                     TVIPEPLIVGKTIIVSCGSYTEKLGKLLVEKNDKAWTVGTYQLIDINDSVPEDREILP
                     IVNYFKLIVEREYLRQYDLGFDEVLAYTPFHFTASNEIGDEHREEPLGTLICDAYRYA
                     VEQAEGDSYEEITAALVPSGVVRGSFVKGDISVADAFIVSSLGIGKDGIAGYPLISVY
                     LTGKELKTMCEVDASIQPIMSAAQLYITGLTYTFNPHRLIFNKVTDVALINKDGIKEK
                     IDDKKLYRVVTGLYSGQMLPVINDKSFGILSVVPKTKDGEVIRDFEAQIIYDLRDGQK
                     KEVKEWVSLAQYLQSFPKIDGKSIVSNYYAQAQGLKNVDASKNIFKIIASPNLISLIV
                     YAIVIIISILIVWIFVVIIKRIHKK"
BASE COUNT         2445 a         1838 c         1500 g         2439 t
ORIGIN      
        1 ttcgtataat tcaattgcta attcataatc ttgaactcgc aatgcttcaa gtaaggcaca
       61 acttgtctca aacaaagcag taattgcaag agagcctgtt atccccttaa aagaatggac
      121 gctatcttct gcagtacccc aattttcttc atccatattc aatacaatcg acttatacac
      181 agggtcgtcc aaaaatttca ctaagtactt ttctagtaat tctctgttcc cgctaaagcg
      241 aacaagtgca tcatcaacat cgatgccccc ttgttttagt ttttcaaaat tcatacctaa
      301 agtatagcat ctcttaagat gttgtcaagt ttgcacaaaa attttatgaa acaacaccta
      361 tttctttaaa gtttgacatc aaagcatatt gactaaagtt tgatataaaa ctaatataag
      421 tcagaaaata tatcaaagat ttgaggagtt tttatgttag caaaaaaacg aatttttgcc
      481 ttagtttgtg cagttcttat tctttttgtt tcatgcacaa aaaatgaaaa aaagacagat
      541 agcctatcta tagcgatttt tgtacctggt gtagttgcag gaaatgctgt ctacgaaatg
      601 tttgtctcgg gaagtgaaaa agctatagag agctacaaga caaaagaaag tgcaaagagt
      661 cttaagtatc aggtaattga agcagggacc aatcagtccg agtggccagc aaaactgact
      721 gccctctgtg ccagtgagca atgggacctt attttttcat caaacccggc ccttcctgat
      781 attttggaac cgatactaaa agattttccg aatcaaaaat gtatcatcct cgattcctat
      841 aaaacaggaa tagcaggtat gcaaacggtc ttgtacaatc aaaggcaaca agcttatctt
      901 gcaggttatg ctgcagcaat ggtaacaaca gcaaaagatt tgccctatgc aaatagtgca
      961 aaaaaaattg ctctcattgc ggcgcaagaa tatcctgcga tgaacgatat tatgtatcct
     1021 ggttttatag aaggcgcaag ggcatacgac ccagaaatcg aagttctatt tcgcattgtg
     1081 gggaattggt ttgatgcatc gaagagctat gaaatagcaa gctccttgta cgacctcgga
     1141 gtagacgtca tcttacccat agccggtggc gcaagtcagg gagttatttc ttgtgcaaaa
     1201 gataagagct tttatatttc ttggtttgac aacgagggct acgaaaaagc tcctggctat
     1261 gttatttcaa gtgcgatgat gatgcaagaa aaaatggctg agcaagtttt gggaaactat
     1321 ctcgatggtc gagctgaatt tggaacagca aatatttacg gtatggaaga tggttttatt
     1381 gactttgtct tgcacgaacc gacaagcgat aatcctgagc gtgtccaaaa aatagcagaa
     1441 gtctacgatg caattaagac aaaaaggctt gtactcgagc ccagtctata atattatagt
     1501 actatgtttt atcttagagc cacgaatatt agcaaaaaat ttacgcataa aaaggctgtc
     1561 gatggctctt ttctaagtgt tttttctggt caaattcatg ccctgctcgg tgaaaacgga
     1621 gcagggaaat caacccttgc tgcgatatta agcggggtaa tccaagcaag cgagggcgaa
     1681 atagaaattg gttactctcc ctttcgaacc gaatctctca agctaaaacc agcacagata
     1741 caaagtccta aagatgcaca agagagggga attgtcttgg tacaccaaag accgatactt
     1801 tctgagtctt taagcgtttt ggaaaatact gtactgggag accttcatcg aagaatattt
     1861 ttctcaaaaa aaaagcttaa aaaaacactg aacaagattc tcaaagattg ggagatagaa
     1921 ctcgacctca ataaaaaggg aagtcagtta aatgccgaag agagtttttt tgcagagctt
     1981 ctctccgcct tgtataaaaa cccaaaactc ctcatcttgg atgagccaac ggcattattg
     2041 cccgaaaaaa aaagaaaggc tttttttatg agccttaaaa ctcaggcaga aaaaggattg
     2101 gctgttttac ttattacgca taatctgcag gaagcgcttg ataatgcaaa ttttatcact
     2161 gtcttaaaaa aaggaagagt tgtcttggca aaacaaaata gccaaaacaa aaggatacag
     2221 atgagcgctc ttgaaaatgc gctcttcact tctgatataa acttaaaaca ggaaaaaata
     2281 cagaataaaa aaaatgcaga aaaaaatatc tcgctacaaa aaacacatac taaggggcaa
     2341 ataaaaaagg cacaaaagga tgaggcaaaa aaaatatttg cactatcaaa agttttttat
     2401 actggtaaaa aatatccttc cttattcaat atttcttttt cggtttatga aggtgcgatt
     2461 tgttatattt atggacaaaa agcaagtggc atagaaagcc tagaagaaat tataacagga
     2521 attcaaaaga tgccctttca gggaactatt ttttttaacg aagataattt tacaaaacaa
     2581 ctgagcccgc gtattttgcg aaaaggtggt gctgccatta tttcaaccaa taaaaactat
     2641 cgtgcttcta acccttcgct gagcattgta gaacttttaa gcgtttacaa agctgtcgat
     2701 gaaaaagctg aagatgccga aactttttca aaaaatctca ttcagcttga aaaaatgccc
     2761 atagagattc acgaaccagt atcgaacctt tctgggggaa tgcttcagcg acttatttta
     2821 gaacgggaac tttccacaga gccaaatctg cttttgtttt ttgaaccagc tcagggttta
     2881 gatgcgcagc gtttagcctc ccttgccaat cacctgcgcg cccttgccga tatgggaaag
     2941 gcaatcttac ttgtttcagc taatcgagaa aaagaatttt cagccattgc cgatatacaa
     3001 tacgaaatgc gcggaggaag tcttgtatga aaaaagcatt gtcaaaaatc ttttgcacta
     3061 tcccaaggtc gcaaaaaaaa tttccatata caaaggccct cgcctcactc gccctcggtt
     3121 ttctcgccgc aggcatcctc gtcttttatt tttcctccta ctcatttgcc gcagtcatag
     3181 atttttttct cagccccttt tcttcggtgt tttttttcgg cagcatgctc gcaagtatga
     3241 gcttactcct cgttggctcc ctcggctcct cagtcgccct cctctctggg aattacaacc
     3301 tcggtggtga aggacaagtc tacctcggcg gccttatcac cgccctcata ttaagtaagc
     3361 tcgacatttt ttttgctcct cttttggcaa tcatccttgt cattatcgcc tctgcactct
     3421 taagctttat acccatcatc ctaaaactct acagaggagc aagcgaattg ctcacgagtt
     3481 ttttattgtc tgccgccctt ataccgctca tcgattgggc aattgctatt ccgctacgaa
     3541 acaagggaca aaatctcttg gcaacaagcc caatattggc tcgatttgaa atgacaaaaa
     3601 ttcttccgcc ttcttcattg agcattgttt tttttctttc tatacttctt gtttttattg
     3661 catggagaat cttatataga acaaaggaag gcgagctttt tcgtataagc ggcaaggcag
     3721 aagaattttc cttctacgca ggatttaaaa caaagcgcta caatataatt gcgatgagca
     3781 tttcgggcgc cttacacggc ttagttggct atatcgcggt agtgagcata catcatacct
     3841 gccactcagg cttttatctc ggcttgggat ggaatgccct atctgttgca ctcattgcaa
     3901 aaagttatcc gctcttgctc atccctgcat cttttatact cgcctatctt ttttctgcaa
     3961 gcgattacgc tgtattattt tatagcatgc ccttcaacgc aagctttctc atacaaggga
     4021 ttgttctttt tgcaatcgct gcatcacata taggaggaaa aaagaatgga taatattttt
     4081 acaattctca ttctcagctc ccccctcctt cttgcatcct tcggcgccct tgtaagcgaa
     4141 aatgcaggag ttatggcggt atttattgac ggcattattt ctctttcagc ttttttattt
     4201 tttagcttta ccgtcttatt tcaaaaccca gtttatgcag ctattctcac gctttttctt
     4261 tgcagtattt tactgcttgc ttcctccttc tatgcgacaa agacaaaggc aaaccccttt
     4321 ttagttggac tcgctatcaa tttactaagc accggactca ttgcactttt ttcaagcatt
     4381 ttttttcaca cccaaggagt acttaccaat catgcttttt tcaatagcta tacatcaaac
     4441 ctttcacgca gctggggaac agttttaagc tttgccctcg tacttttttt tagcctcttt
     4501 tataaaaaaa caaaaccagg cttggcttta caaatcaccg gcttagaaag cgaagtcctc
     4561 tatcaacaag gattaaagcc ccgctttttc cgccattttt cctggattat tgcaagcctc
     4621 tttgcctccc tcagcggcat cctcctcgta ctccgcctct catcctttgt ccccaatatt
     4681 tctgcaggcc gcggttggat agccctcgct attgtcttct ttggaagaaa aaaaccactg
     4741 ggcgtcctgc ttgctgtcct cgtttttgca tctgcagaat gggcctccgt ctttttacaa
     4801 aacacaggca tgcttgaaaa tgtttcatcg ggtattttta ttgcccttcc ctatctcgta
     4861 agcctcatcc tctttgcgct tcttccacaa aaaaaacaat aaaaactcga taaaaaatca
     4921 ataaaaaaaa gccagcaaaa aaactgctgg cttttcatat ctaatcgaaa ttaaaaaact
     4981 taatagcttt ttagtttttt gtattgggcg ttttcgccaa ggaattcttc tatgcgcaag
     5041 agttgattat attttgcaac tcgttcgctt cgtgcgggag ctcctgtttt tatttgccca
     5101 gcatttaatg ctacagcaag atcggcgatt gtggtatctt cggtttcgcc agagcgatga
     5161 gaaataaccg cagagaaacc tgcattgtgt gccatcttaa tagcttcaag agtttcagaa
     5221 acggtgccga tttggttaag cttgataaga attgagtttg cggatttttc tgcaatgccc
     5281 cgtgaaagac gttctgtatt tgttacaaat aaatcatctc caacgagttg caccttgtcg
     5341 ccaagttctt gcgtcagcaa attccagcct tcccagtctt cttcatcaag agcatcttca
     5401 atagaaaaaa taggatattt ttctaccaag tccttccagt gcgcaaccaa ttcttttgcg
     5461 gtataggttc taccgctctt tggcatcttg tattcgccaa cctttccagc cttccattca
     5521 ctcgccgctg catcgagcgc aagcacaaag tcagagccag ctttgtaacc agcctttttt
     5581 attgactcga gtataatatc gagcacttcg gcatcactgg acaagttcgg cgcaaaaccg
     5641 ccttcgtccc caatgccacc gagcaagccc ttttccttaa gcgttcctgc aagcgcatga
     5701 taaacctctg tacaccagcg caagccctcg gcaaaggatg aggcaccctt gggcataatc
     5761 ataaattctt gtacgtcaac ggtatttgca gcatgagcgc caccgtttaa aatattcatc
     5821 ataggtacgg gaagcatcgt tgcatttacc ccaccgagga aacgaaagag cggtatgccc
     5881 aaagattgag ccgatgcacg agctgctgca atggaaacag caagaattgc atttgcacca
     5941 aactttgact tatcctttgt cccgtcagct tcgatcatag ccctgtcgat cgcataggta
     6001 tcgtaaggat tttttcccac gagcaaatca tttatagtgg tattaatcgc agcaaccgcc
     6061 tttgttacac ccttaccacc atagcgcttt ttatcagcat cgcgaagttc aagcgcttcg
     6121 aaaatacccg tcgaagcacc gcttggagct gcgccaaaag caatagttcc atcgcaaaga
     6181 tgcacctcag cttcaactgt tggatttccc ctggaatcaa taatttctct accaattact
     6241 ttggtaatta ctacattatt catttttcct cctatttctt atgtattctt ttaataatca
     6301 caacaaagat ccatacaata agaatactaa taattatcac aatcgcatag acaataaggc
     6361 taattaaatt tggcgaagca ataatcttaa aaatattttt acttgcatcc acatttttta
     6421 agccctgtgc ctgtgcataa taatttgaaa caatactctt tccatcaatt tttggaaaac
     6481 tttgtaaata ctgagcaaga gatacccatt cctttacttc ttttttttgt ccgtcacgca
     6541 ggtcataaat tatctgcgct tcaaagtctc taataacttc tccgtccttt gtttttggca
     6601 caacagacaa aatcccaaaa gacttatcgt ttataaccgg aagcatttgc cccgaataca
     6661 aacccgtaac aacacgatag agtttcttat catcaatttt ttctttgatt ccgtctttat
     6721 ttataagggc aacatcggtt accttgttaa aaataagtct atgtggattg aaggtatagg
     6781 ttaaaccagt aatgtatagc tgagcggcac tcataatagg ttgaatcgaa gcgtcaacct
     6841 cgcacattgt tttaagttcc ttccctgtca aatacaccga gattaaagga tagcctgcaa
     6901 tgccgtcttt tccaatgcca agcgaactta cgataaaggc atctgcaacg ctgatatctc
     6961 ccttgacaaa ggagccacgc acgacgccac ttggcacaag cgccgcagta atttcctcgt
     7021 aagaatcgcc ctccgcttgt tcaaccgcat accgataggc atcgcatata agcgtcccaa
     7081 gcggctcctc cctgtgctca tcaccgattt catttgaagc ggtaaaatga aaaggcgtat
     7141 aggcaagcac ctcatcaaaa ccaaggtcat actgacgtag atattctctt tctacaatga
     7201 gcttaaaata attgacaata ggcagaattt ctctatcctc tggtacagaa tcgtttatat
     7261 cgatgagttg ataggtcccc acagtccaag ccttgtcgtt tttttcgaca agaagttttc
     7321 cgagtttttc tgtatacgag ccacaagaaa caataattgt ctttcctaca atgagcggct
     7381 caggaatcac cgtatgacta tgccccgaaa taataacatc gatctctggc acctttttgg
     7441 ctaaaatttc atcttcagtg tttggactct taccttccgt tcccgaatgg gaaagacaga
     7501 gaatcatgtc aacattttca tttgcttgaa tatctgaaac aatgcgccgt gcattttcta
     7561 tataatcagt aaaagtaacc tctgccatag gagcattcga ggctgagtcc ttccccataa
     7621 ccgcaaaaag cgcaatcgta tagccttttt tctcaagtag aatgtattcc tttgcaggat
     7681 agtttgccaa ggcagcttcg agctcagcaa gagacggcgt taaattgccc gctgcatttt
     7741 ttggaaatgt aacatttcca acaagaagtt caggaagctt gtctccactt gcctttgcag
     7801 tatttaaact ttttgcaagc ccactggcac gaaagtcaaa ctcatgattt ccaagcgttg
     7861 ttgcttcaaa gccaattttt cccataatgc gcagctgtgg actgtgggta gaaaaaatag
     7921 tttgaaaaag cgttcccata gaataatcac ccgcatcaac aagcaaaagt tccgagtctt
     7981 ttttccgctc ttcctttacg agacttgcaa tacgcgtaag cccataggta ctcatcgact
     8041 gtccattttg caaggtatta ctaggcgtta aatggtcgtg catatcgtgc gtaaaaagaa
     8101 tagttagaga gccatcacct gcaactaaga caaatgataa agacaaaaga acaaaaagaa
     8161 aaagcaacct tttccaaatg cgcggaaaat tttcccctga taaaaaaaac atttctataa
     8221 ca
//
LOCUS       DAOSSW010000076         8143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_8342_length_8143_cov_6.362018, whole genome shotgun sequence.
ACCESSION   DAOSSW010000076 DAOSSW010000000
VERSION     DAOSSW010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 8143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8143
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8342_length_8143_cov_6.362018"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PKW26_06750"
     CDS             complement(<1..927)
                     /locus_tag="PKW26_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOQ93301.1"
                     /translation="MKKKCVVVFLALLAIFSFSQEKTADIIVLLDNSSSMLPYFSEIN
                     EKVLQGICENFIRLEDSFHLLTFTEKPYLEVSQKIRSQSDVEKIVARFSLLYPLGSYS
                     NFIEALSYSRQYINSLNAYTQKVLVIISDGVFKPPMSSQYYGEEPQVLENAIQTALNA
                     LQGQGVLVYFIKAPFPENVMVKDFTGRLTTAKLHESEGDFVLEGKSDAFNEELFYEYA
                     TNLQNNNGVVSADLDDSQAIKGDFIASAFQLPRISTKENLGKKNYQFRLPLYIENTSD
                     AAIHLQLDRILLADTNILKSTSFISVKPGRTKK"
     gene            complement(1008..3695)
                     /gene="greA"
                     /locus_tag="PKW26_06755"
     CDS             complement(1008..3695)
                     /gene="greA"
                     /locus_tag="PKW26_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOQ93302.1"
                     /translation="MSEALISSVQEMLNEEKWTRATISNYTKNHFLELSAVVEKAKEQ
                     NVISEVKEICDEHLAHSKNSIIALYLSGMLGLKLKSLDNSALSSLVTIFVDNHKNSIV
                     IFLCKSILEEEETNKFALRTLADAYKEENAEAIWEVYEQIVRIDYEEADIAKLLAEQY
                     EKEAKRDDAIDYYKKALLRYVNKKSVNQLKEMWTKLVELIPEEIDFFYLVQRKIAKTI
                     NADKSALLMQELYTYYKDSKDWNTAIDILKLVLSIDEKDGWARREIVDCFKEKYADHS
                     QLDEYIKISNLTQNWRNVFEAISDFEKHISFDTKNFVFHRSWGVGIIRKVESDEIEIN
                     FGRKFGVRSMSLKMAVDALQPLHGEHIWVLKATQSREELAKKAKEDKVWMLKTIIKSF
                     NNNCDFKRIKAELVPGILEQSQWTSWSTAARKILETDASFGVNPNDISLYTVRDRAIS
                     QEEKLSNEFKAQKQFFARIDILMKFLADADISSELFTEMFSYFVGFLRAINIVNEQTL
                     ASFLVVKRIVAQHPHLDPGITFTFAQLFEELENPNASYLALKDTKNTFLRKDFLSSIK
                     SLLPNWSDIYIRLFPTVLQIDLLNALISAGHVEKVKQMSINCFDNYKDYREAAIFFFS
                     ECQEEEWFTDVPYEKQLITMINIIDLTFREIANQRDTTLNRKINKQVQNLLFKKNTLL
                     EYLLENDTDTITRLYTVINDIRDLDPAIKSNLRNKIMEKHTDFKFYGAEEKTIVAKGL
                     IVTSKMLEVKKQQLEHIATVEIPENSKEIGEAMALGDLRENAEYKAAKEKQVQLNAQA
                     TRLQDEIDRAQIFDPTTLTTIRVSFGTMVRLKNNETNEEEQYTILGPWESDPDNNVIS
                     YMSPLGNAFLGAKEKEKLDFVINQRDCSYTVLSISEAKF"
     gene            complement(3710..4642)
                     /locus_tag="PKW26_06760"
     CDS             complement(3710..4642)
                     /locus_tag="PKW26_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93303.1"
                     /translation="MSSRSDSQLQNVYELLKNAKPLEAKQELESILSQDLENTDILFA
                     LKVATYWANEMKMIDSFSNGYEKGEQLISKWKHFYSLLKHDIPQHESLLFFIQQGVFR
                     YCLDSFSSILKESRTQDKADVYARMGLCYKKLGDYTTSLRYLKDANKAAPKKADILAQ
                     MADCYALCGEEIPAKALFREAFFIEPKQIDLELLESELFYKLHEITLASKQKDSNLAA
                     WIPIYGVLYGILTIKRELKSLEVGQLKQAIYALEKEYKEAEVAKDDIKALLINHYFWL
                     IDHYIAANEGRECVQEVLLKIKIIDSTIYEQYCA"
     gene            4836..5471
                     /gene="rpe"
                     /locus_tag="PKW26_06765"
     CDS             4836..5471
                     /gene="rpe"
                     /locus_tag="PKW26_06765"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOQ93304.1"
                     /translation="MKKPILAPSLLASDFTKLGEAIQKIEEVADAFVHIDVMDGSFVP
                     QISFGQPIVQAIRPLTKRIFDVHLMIDNPQAHFKSFVDSGADYLTFHYENTVHHHRLI
                     QEIRDLGVKPGISIVPSTPVSALEEILPLVDLVLVMSVNPGFGGQKLIPSCLNKVKNL
                     KKTRQKSSYSYIISIDGGVNSETLPSVLEAGADLVVSGSSFFSGDLPWGKA"
     gene            5544..6491
                     /locus_tag="PKW26_06770"
     CDS             5544..6491
                     /locus_tag="PKW26_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93305.1"
                     /translation="MQKKSMKQIIILFLCFFILMHLSVFSQEGIASRLDEYAPLFAQQ
                     GIKAYEENNWELAIHFFKRATTFPSTSTQELWYMLIMSELYAEKYEDLLLDVYEFKKN
                     FPGSYLTSFIEYQEGRAHYYLKEWDLAISVLSDFCVAYPEHELYASSLFWIAETLYAM
                     HYYSLAASFYERIADEYPKSVKMTEVLYRLDVIHQREKEEKLLLLLQATSEEYLSSKE
                     EYERQLRQFQTEEAIGLRKQLNDAQAKIAQLEFDKNEADLHSSLQAEKIVQLQSILGN
                     IEKNEAKDASRDLINQIQMLKEKAAQIKGELEENTLGEK"
     gene            6510..7634
                     /locus_tag="PKW26_06775"
     CDS             6510..7634
                     /locus_tag="PKW26_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93306.1"
                     /translation="MKKKLTIILCFFVSLLAFAAQQEKRFGNLKVVLHDVSLTHSLYF
                     IDAQTEKMIPVFDTIDASSNNRFYLQTDLMMYSLTKSSGVIGSQQFTENGFQVLYEIT
                     DLATILFDYVFSASPGRNTDDFDLLSLSVQIVNKAKKTQNFALKAVFDTHLGEKTQTH
                     FSTQLEPIIKTEMNFSRLVEEAWIKSSDGLRSIQFNFPESVSFPSFARLANKDFVLAQ
                     TWDINGLTGRSFNSVYSYNNSAVALYWAAQKTDPEKTALFQLSLSFAQDSLNPYRVLS
                     IKTETQNISDPTLEKPSLDTTIIEEKSGLEEPLDDLSVQAENPYAYTKSITSDQLDKA
                     YIEALLAKIEALENEDTSLDRTELLKLNAELDAILEFLRK"
     gene            7643..>8143
                     /locus_tag="PKW26_06780"
     CDS             7643..>8143
                     /locus_tag="PKW26_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93307.1"
                     /translation="MASVDIIKKAQSLLAKKEYAKAANLLEPNIISFSNSFQFHYTLG
                     LSFLYLGDIGGAELYFKKARNIKMQDINLILAQACIFLRKGQIDRSLSYCLDALDIDA
                     QNPYARRFLLLLKKYGDTEIISQWIYNGKILRFYPPLPKKNPVFSILAVFLTLSILGL
                     GVFFFFQ"
BASE COUNT         2416 a         1442 c         1492 g         2793 t
ORIGIN      
        1 tttctttgta cgtcctggtt ttacagagat aaagctcgtt gatttaagga tgtttgtatc
       61 tgccagaaga attcgatcaa gttgcaagtg aattgctgca tctgaagtgt tttctatgta
      121 gagaggtaaa cgaaactgat agtttttttt accaagattt tcctttgttg aaattcttgg
      181 cagttgaaaa gctgatgcta taaagtctcc cttgatagct tgactgtcat ctaaatctgc
      241 gcttacaact ccattattgt tttgtaaatt tgttgcgtat tcataaaaaa gctcttcatt
      301 aaaagcatcg gattttcctt ccaaaacaaa gtcgccttcg ctttcatgta gttttgctgt
      361 tgttaagcgc cctgtaaaat cttttaccat gacgttttct ggaaagggtg cttttataaa
      421 atacactaaa acaccttggc cctgcagtgc atttaaagcg gtttggattg cattttccaa
      481 aacctgtggc tcttcgccat agtattgact agacattggt ggcttaaaaa caccatctga
      541 gatgatcacc aaaacttttt gtgtataggc attgagagag tttatgtatt gtcttgagta
      601 ggatagtgct tcaataaaat ttgaatagga gccgagcgga tataaaagac taaatcgtgc
      661 aacaattttc tctacatcac tttgacttcg tattttttga cttacttcta gatagggttt
      721 ttcagtaaaa gtaagaaggt gaaaagagtc ttcgagacga ataaaatttt cgcaaatacc
      781 ttgaagtact ttttcgttta tttcggaaaa atagggaagc attgagctcg aattatctaa
      841 aaggacaatt atatccgctg ttttttcttg cgaaaaagaa aaaatagcta aaagagctaa
      901 aaaaacaact acacattttt ttttcatata atacaactcc aagtgttata attagatttt
      961 cttttgccta attataacac ctttaaggat tattcacagt acttttttta aaatttggct
     1021 tcagaaatag aaagaaccgt gtatgaacaa tctctttgat ttataacaaa gtcgagtttt
     1081 tctttttctt ttgcaccaag aaaggcgttt ccaagcggtg acatatagga aataacgtta
     1141 ttatctggat cagattccca tggtcctaag atggtgtact gttcttcttc atttgtttca
     1201 ttatttttaa gcctaaccat tgtgccaaaa gaaacacgta ttgttgtaag tgtcgttggg
     1261 tcaaagattt gcgctctgtc tatttcatct tgtaagcgtg ttgcctgtgc atttaattgg
     1321 acttgctttt ccttagcagc cttatattct gcgttttctc gcaagtctcc aagtgccatc
     1381 gcttccccaa tttcttttga gttttctgga atttcaacag tagcaatatg ctcgagttgt
     1441 tgttttttaa cttcaagcat tttacttgta acaatgagcc cttttgcaac aatcgttttt
     1501 tcttctgcgc cataaaactt aaagtcagta tgtttttcca taattttatt tcgtaaattg
     1561 gattttatag caggatcgag gtcgcgaata tcatttataa ccgtgtaaag acgagttata
     1621 gtgtctgtgt cattctcgag tagatactca agtaaggtgt ttttcttaaa taaaagattt
     1681 tgtacttgtt tgtttatttt tcgatttaag gttgtatccc tttgatttgc aatttctcta
     1741 aaagtaaggt cgatgatatt gatcattgtt ataagttgct tttcatatgg gacatcagta
     1801 aaccattctt cctcttggca ttcgctaaag aaaaagattg cagcttctcg atagtcttta
     1861 taattatcaa aacaattgat agacatttgt tttacttttt ccacatgacc tgcactaatg
     1921 agggcgttta aaaggtctat ttgcaagaca gttggaaata gtcggatata tatatctgac
     1981 caattgggga gtaagctctt tatgcttgat aaaaagtcct tgcgtaagaa ggtgtttttc
     2041 gtatccttga gggcgaggta gcttgcgttt gggttttcaa gttcttcgaa aagctgtgca
     2101 aaggtaaagg tgataccagg atcaagatgc ggatgttgag caactatgcg ctttacaact
     2161 aaaaaagaag caagtgtttg ctcgtttacg atatttatag cacgtaaaaa gccgacaaag
     2221 tatgaaaaca tttcggtgaa aagctctgaa cttatgtccg catctgccaa gaatttcatg
     2281 agaatatcga tacgcgcaaa aaattgtttt tgtgctttaa attcgttgga aagtttttct
     2341 tcttgagata tagctctatc tctcacagta tagagagaaa tatcgtttgg atttacacca
     2401 aaacttgcat ctgtttcaag gatttttcgc gcagcagtgc tccagcttgt ccattgactt
     2461 tgctcaagaa tgcctggcac gagttcagct tttattcgtt taaaatcgca attattattg
     2521 aaacttttta ttatagtttt gagcatccac accttatctt cttttgcttt ttttgcgagt
     2581 tcttctcgag attgtgttgc tttcaaaacc caaatatgtt caccgtgaag tggttgcagt
     2641 gcatcgacgg ccattttgag cgacatgctg cgaactccaa actttcggcc aaagttaatt
     2701 tcaatctcat cgctttcaac ctttcgaata ataccaacac cccaagaacg gtgaaagaca
     2761 aaattctttg tgtcaaagga tatgtgtttt tcaaaatcag aaatagcttc aaaaacgttt
     2821 cgccagtttt gtgtaagatt tgaaatttta atatattcat cgagttggct gtggtcagcg
     2881 tatttttctt tgaagcagtc tacaatttct cgccttgccc aaccgtcttt ttcatcaata
     2941 cttaaaacaa gttttagaat atcgatggcg gtattccagt cttttgagtc cttatagtaa
     3001 gtgtagagtt cttgcataag aagggcgctt ttatcagcgt ttatagtctt tgcaattttt
     3061 ctctgtacaa gatagaaaaa atcgatttcc tcaggtatga gttcgacgag ttttgtccac
     3121 atttctttta attgattgac cgattttttg tttacatagc gaagaagcgc ttttttataa
     3181 taatcaattg catcgtcgcg tttagcttct ttttcatact gctctgcgag aagttttgcg
     3241 atatctgctt cttcataatc gatacgtact atttgttcat atacttccca aatagcttca
     3301 gcgttttctt ccttgtatgc atctgcaagg gttcgaagtg cgaatttatt ggtttcttct
     3361 tcttcgagaa tagatttgca aagaaatatc acaatgctgt ttttgtgatt gtcaacaaaa
     3421 attgttacaa gggaagagag tgctgagttg tcgagcgact tgagctttaa tcccagcata
     3481 ccggaaagat acagtgctat gattgagttc ttcgagtgtg cgagatgttc gtcgcaaatt
     3541 tcttttactt cgcttatgac attttgctct tttgcttttt caacaacggc agataattct
     3601 aaaaaatgat ttttagtata atttgaaatg gttgctcttg tccatttttc ttcgtttagc
     3661 atttcttgta ctgatgatat gagagcttca gacattggtt actcctgttc taggcacaat
     3721 attgttcata tatcgtgctg tctattattt taatttttaa taaaacttcc tgtacacatt
     3781 cacggccttc atttgccgct atatagtgat cgattaacca aaaatagtga ttaatcaata
     3841 aggcctttat atcgtccttt gctacttccg cttctttata ctctttttct aaggcataaa
     3901 ttgcttgttt gagctgtcca acttctaaag actttaattc gcgtttaata gtaagaatac
     3961 catataaaac gccataaatt ggtatccagg ctgcaagatt ggaatctttt tgcttcgatg
     4021 cgagtgtgat ttcatgcagt ttgtaaaaga gctcggattc caaaagctct aaatctattt
     4081 gtttaggctc aataaaaaaa gcttccctaa ataaagcttt ggcaggaatt tcttccccac
     4141 agagcgcata gcaatcggcc atttgtgcta gtatatcagc ttttttagga gctgccttat
     4201 ttgcatcctt taagtagcga agcgatgttg tgtaatcgcc gagtttttta taacaaagcc
     4261 ccattcttgc atacacatct gccttatctt gcgtcctgct ttcttttaat atgcttgaaa
     4321 acgaatccaa gcagtagcga aaaactcctt gttgtataaa aaatagtaaa gattcgtgct
     4381 gaggaatgtc atgttttaaa agggaataga aatgcttcca tttagagatc agctgttccc
     4441 ctttttcgta gccattcgaa aaagaatcta tcattttcat ctcatttgcc cagtatgttg
     4501 ctaccttcaa ggcaaataaa atatctgtat tttcgaggtc ttgcgataag atgctttcaa
     4561 gttcttgttt tgcttcaaga ggttttgcat tttttaacaa ttcatacaca ttttgaagct
     4621 gtgaatcaga cctgctactc ataattactt actattatac ttaaaaactc tctttagtca
     4681 atctagaaag gaaaaattgc gaaaaatggg ctaaagttta taatacgttt tattgcttta
     4741 gtcaatactc tatttttgct tttttcaaaa aattgacttt tatagaaaaa ctttaaggca
     4801 tttacatatt tctatctctt tgctactata taagtatgaa aaaacctatt ttagcgccat
     4861 ctttgctcgc ctctgatttt acgaaacttg gtgaagccat acaaaaaata gaagaagtag
     4921 cagatgcctt tgtgcatatc gatgtgatgg acggcagttt cgttccgcaa atatccttcg
     4981 gtcagccgat tgtacaagca attcgcccgc tgacaaaacg catttttgat gtgcatctta
     5041 tgatcgataa tccacaagca cattttaaaa gctttgttga ctccggtgca gattatctta
     5101 cctttcatta tgaaaacacg gttcatcacc atagactgat tcaggaaatt cgcgaccttg
     5161 gggtaaaacc aggtataagt attgtcccct caactcccgt ttctgccttg gaagaaatac
     5221 taccgcttgt cgacttagtg cttgtcatgt cagtgaatcc cgggtttggc gggcaaaaac
     5281 tcatcccttc ttgcttaaat aaggtaaaaa acttaaaaaa gactcgccaa aagagctctt
     5341 attcgtatat aatatcaata gatggtggtg tcaattctga gacacttcct tctgttttgg
     5401 aagcaggtgc cgaccttgtg gtttcgggtt cttctttttt ttcaggcgat ttgccttggg
     5461 gaaaagcata ggagtttaat ataaatcctg tttttacaag agtttcaaaa gccggtaaaa
     5521 gtctgtatgt taaggatgta cgtatgcaaa agaaaagtat gaagcagatt attattcttt
     5581 ttctttgttt ttttatctta atgcaccttt ctgttttttc tcaagagggg attgcttctc
     5641 gtttagatga atacgctcct ctttttgctc agcaaggcat aaaggcatat gaagaaaata
     5701 attgggagct tgcgatacat ttttttaaaa gagctacgac cttcccatct acttctactc
     5761 aagagctctg gtatatgctg attatgtctg aactgtatgc agaaaaatac gaagaccttt
     5821 tattggacgt atatgaattc aaaaaaaatt tcccaggtag ctatttaact tcgtttatag
     5881 agtatcaaga aggaagggcg cattattatt tgaaagaatg ggatttggct atttctgtat
     5941 tatcagattt ttgcgtagcc tatccagaac acgaactcta tgcatcgagt cttttttgga
     6001 ttgcggaaac cctgtacgct atgcattatt attccttagc ggcgtctttt tatgagagaa
     6061 ttgccgatga atatccaaaa tcggtaaaga tgactgaagt tttatatcga ctggatgtga
     6121 tacaccaaag agaaaaagag gaaaagcttc tgcttctttt acaagctact tcagaagaat
     6181 atctttcttc caaggaagag tatgagcggc agctacgcca atttcaaaca gaagaggcaa
     6241 tcggtttgcg taagcagctc aatgatgcgc aggctaaaat tgcacaattg gagtttgata
     6301 aaaatgaagc agacttgcat tccagtttgc aagctgaaaa aatagtgcaa ttacaaagta
     6361 ttctcggtaa tatagaaaag aacgaagcaa aagatgcttc gcgggatctt ataaaccaaa
     6421 tacaaatgct caaagagaag gcagcacaaa taaagggcga acttgaagaa aacacccttg
     6481 gtgaaaaata agctaaagag gagaataaag tgaaaaaaaa attgactatt atcctatgtt
     6541 tctttgtttc tttgttagcc tttgcagctc aacaagagaa gcgttttggc aatttaaaag
     6601 ttgttctaca cgatgtatct ttgactcatt cgctttattt tattgatgca caaacggaaa
     6661 aaatgattcc cgtttttgat actatagatg catcatcaaa caatcgtttt tatttacaga
     6721 cagatttgat gatgtacagc ttgacaaagt cttcaggagt gatagggagt caacagttta
     6781 ctgagaatgg ttttcaagtt ttgtatgaga ttacggacct tgcaacgata ttgtttgatt
     6841 atgttttttc tgcctcgcct ggtagaaaca ctgatgactt tgatctactt agtctctccg
     6901 tgcagattgt aaataaggca aaaaaaacac aaaactttgc acttaaggct gtttttgata
     6961 cgcaccttgg tgaaaaaaca caaactcatt tttctacgca attagagcct atcattaaaa
     7021 ctgaaatgaa tttttcccgc ttagtagaag aagcttggat aaaaagcagc gatggtttgc
     7081 gcagtattca atttaatttt cctgaaagcg tctctttccc atcctttgct cgacttgcta
     7141 ataaggattt tgtactagca caaacatggg atataaatgg cttaacgggg cgtagcttca
     7201 attctgtata ttcatataac aattctgcag tcgccttata ctgggctgca caaaagactg
     7261 accctgaaaa aactgctctt ttccaactgt ctttgagttt tgcacaagac agcttaaatc
     7321 cctatagagt acttagtata aaaacagaaa cccaaaacat ttctgatcct actttagaaa
     7381 aaccaagtct tgatacaacc attatagaag aaaagagcgg gcttgaggaa cctttagatg
     7441 acctttccgt gcaggcagaa aatccctatg cgtatacaaa atccataacg agcgatcaac
     7501 ttgataaggc atatatcgaa gcgcttttgg caaaaatcga agccttggaa aatgaggata
     7561 cgtctttgga tcgaacagaa ttattaaagt taaatgcaga acttgatgca atattagaat
     7621 ttttaaggaa ataaaacctt ctatggcttc tgtagatatt ataaaaaaag cacaatctct
     7681 gctggcaaaa aaagaatacg ccaaagcggc aaatctcttg gagccaaata tcatcagttt
     7741 tagtaattct ttccaatttc actatacact gggactttca tttttgtatt taggtgacat
     7801 cggaggggcg gaattatatt ttaaaaaggc tcgaaatata aaaatgcaag atatcaacct
     7861 aattcttgcg caagcctgta tttttttgag aaagggacag atagatcgct ctctttcgta
     7921 ttgccttgat gccttagata tcgatgcaca aaatccgtat gcacgacgat ttttgctttt
     7981 attgaaaaaa tacggggata cagaaattat ttctcagtgg atatataatg gtaaaattct
     8041 acgattttat cctccgctac caaaaaaaaa tcctgttttt tctatcctag cagttttttt
     8101 gactttatct atacttgggc ttggtgtttt tttctttttt caa
//
LOCUS       DAOSSW010000077         8072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_8426_length_8072_cov_5.312087, whole genome shotgun sequence.
ACCESSION   DAOSSW010000077 DAOSSW010000000
VERSION     DAOSSW010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8426_length_8072_cov_5.312087"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PKW26_06785"
     CDS             complement(<1..642)
                     /locus_tag="PKW26_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOQ93308.1"
                     /translation="MQSPITVLSIDIGTSSLKAGLIDTTGSLLAFERERFSKEKPGTM
                     WLDAFLICVKKIFQEQGHAPPAAISISGNGPTLAFLHKDSSGKESADILLWNEPITEP
                     SSTNKITREYQGKSLFIPRILSYFASLSSNGSYATAKPNHPISIIQKLRSLQKIISGP
                     EYLVWQLTGKAHTLLPNPRFAPAYWCDEELEHYGIAKHLLPDFIQIGENSGYLK"
     gene            complement(654..1892)
                     /gene="prs"
                     /locus_tag="PKW26_06790"
     CDS             complement(654..1892)
                     /gene="prs"
                     /locus_tag="PKW26_06790"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOQ93309.1"
                     /translation="MPYSEPTKLAVVACPGGEHFANEVITHLRHMYKHRFNLKRDVIS
                     KRYDTDKDDLVRDINLYNDLHTSDLCVRGHVKKYRAPKFFVDTEFTYFMNGEFKTEIK
                     ECIRGRDVFIFQDVENHQELSLNGGKNKQILSINDHVMSLLVTIDAVRQAGASDISLV
                     LPVYPYSRQHKKKGREGLTASLLGHIYENMGVSRIITLDIHSREIENSFSKAIIENLH
                     ASYQIIRELTKLVNLSADGDDLVVVSPDTGAVDRNKFYSTGLKKPLAMIYKVRDYSQV
                     TQNAKTTNIVSIKLLGDVKGKVAFLADDMLGTGGTLLKAMEFLKEQGATKVIAAISLP
                     FFNGNAIAQFNEAYEKGLFYRIIGTNAVYHEELLKYEWYINSNVSGLFANVIIRIHHN
                     QSLSDLLDNRSIIEKLVKKE"
     gene            2060..2560
                     /locus_tag="PKW26_06795"
     CDS             2060..2560
                     /locus_tag="PKW26_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOQ93310.1"
                     /translation="MDRRHNSLMIGSLVKRLEGILTTKSNNFLKQYNITWSQVQILKY
                     LAVQEELCQEDGKERAVFQKDIEIFFNLTNPIVTGLINRLETKGLLVRETLSRDRRWR
                     RLRITEEGLALEKHIYEEFIRMDGDMMAPFSDSELGDFTLYLERLLHSLSPEILEKSR
                     QNKNLL"
     gene            2805..3836
                     /locus_tag="PKW26_06800"
     CDS             2805..3836
                     /locus_tag="PKW26_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ93311.1"
                     /translation="MSVTIKDIAALAGVSKTSVSFAFNSPERISAETYERIMTIAREQ
                     GYSPDPIARILSNKKTKNIGIVLPQSISVFFQNPYLGELLRGVGSVCDQEGFAVSVLS
                     PFKGIVSQTILNAAVDGILIMGISKDSEVHTTFRQRSMPYVTIDAAEVGDFVNVGIRD
                     VELAKQLMDILLKNGHRKICFCLLQSISSNLQKADTSGTREARLNGILLSAKEYGLTE
                     EEKNDFYFMNVPATLSDAYDKALSVLKKKERPTAIFCMADVQAYGFYRAAQVLGLSIP
                     DDLSIVSFDDLPFTETLAPGLTCVHQSAYKKGRIATKLLFKMIAGQECESAILEANIK
                     HRSSVANIS"
     gene            complement(3889..5469)
                     /gene="malQ"
                     /locus_tag="PKW26_06805"
     CDS             complement(3889..5469)
                     /gene="malQ"
                     /locus_tag="PKW26_06805"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOQ93312.1"
                     /translation="MNMNRASGILLHPTSLPGTPGIGTIGLEARAFVDWLSEANQTLW
                     QVLPLSPTGYGDSPYQSFSTYAGNPLIIDIDMLFRDGWLDEEQTIPPNDINVSSRVDF
                     GKVVNWKIPLLKTAAQQFLNRAKPEDRVHYEAFKNDNASWLDNYAIFMSIKEVYDAKA
                     KTENIWGAMWSNYWPKELASHDPKAVSKWHETHVIECEIQKVIQFFFFTQWNALKEYA
                     NNEGISIIGDVPIFVAADSADVWANQHLFQLSNDGKPLAVAGVPPDYFSETGQLWGNP
                     LYNWDALKKEGYKWWLDRIKALLKMVDFVRIDHFRGFEAYWSVPADEKTAVNGKWIPG
                     PNHDLFIAIKNELGDIPVIAEDLGLITDAVKKLRDDFELPGMKILQFAFDTNEAGKGG
                     FTNTFLPHTYDKNCVVYTGSHDNATMQGWLDAATKDEIQLIGEYLGFEKNDIENFAKN
                     GKLCESLVRLAFSSTANFAVIPLQDIFALNNEARMNLPSTIGGINWQWRMEKTHYNKE
                     KAEWLKKISMLYGRNIRN"
     gene            complement(5474..6304)
                     /locus_tag="PKW26_06810"
     CDS             complement(5474..6304)
                     /locus_tag="PKW26_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ93313.1"
                     /translation="MATLKRKHTSPTTKVVFGIVLIFSIFFFYPMIYVIINSLRESAD
                     ITRFPTGLPNSFYMGNYIKAWNEMEFPKVFLNTFFITTAGVVGIILTSSMCAYMLART
                     TTKTSWIIYSIFTFALVIPFQIIMVPIAVLASDFNLTNTPGIILMYWGLGCPTAIFMF
                     HGFVKSVPRELEESAAIDGAGRFYTFFVIVLPLLKTIIATIAVIDALWIWNDFLLPLL
                     VLREGTIQLAQMRFNGQFLKDYGAMTASLTISSLPIILFYLFLQKYIIKGIAAGAVKG
                     "
     gene            complement(6304..7821)
                     /locus_tag="PKW26_06815"
     CDS             complement(6304..7821)
                     /locus_tag="PKW26_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ93314.1"
                     /translation="MVEPKTKKYGYFIAFILPCVVLYTFFFIYPFFKGISISMTNWDG
                     LTPKSPISLDKTEFETNILDKIKKQSDKDFLLSVYTLDENAHTYSRLNIGGAERNKTE
                     RILKSVKYSPEKNSFVGLKNYKDILSGKVKDTFYPRKKTEIYYNASSSLPLRIEEKKA
                     TKQILKKASDEEKALFLLHYELEDTFYILKNEYNTFYIEEKIWSLPEYSLGSVSDSDI
                     DVYLRKINDISLAEDNKAKENSVDAFLKANLFSQASETIIHETTDEIFNLGTFRNILS
                     TIWTADKIQLGVVGFTFFFALFSVIGINVLAFLLALVLDSDLPGQKIFRTIFFLPNVL
                     SMIIVALIWKMLFYQILPAISGIDLWLSDASKAPWLLILVATWQGCGYYMIVYLAGLQ
                     NIPTDVIEASLIDGASGFQRFRFITIPLIIPAITISLFLTIANALKSFDLIYAMVGPS
                     GYATSTVPFVLDIYYEAFSHKLAGMATSKALLLFLIILLITGLQLQVMKKREVEQ"
BASE COUNT         2443 a         1404 c         1561 g         2664 t
ORIGIN      
        1 ttttaagtag cctgagtttt caccaatctg tataaaatcg ggtagtaagt gttttgctat
       61 accataatgc tcgagttctt cgtcgcacca atatgcaggg gcaaaacgag ggtttggcaa
      121 gagcgtatga gcttttccag tgagttgcca cacgagatat tctggaccgg agataatttt
      181 ttgtaagctg cgtagttttt gtattattga gattgggtga tttggttttg ctgtggcata
      241 acttccgttc gaagaaaggg aggcaaaata gctgagaatg cgggggataa aaagagattt
      301 tccttggtat tcgcgagtga ttttatttgt acttgaaggc tctgtaattg gttcgttcca
      361 aagtaaaata tcggcgcttt cttttccgga gctatcctta tgtaaaaagg caagagtcgg
      421 tccgtttccg gaaatactga tggctgcagg cggagcatgt ccttgctctt ggaatatttt
      481 ttttacacag attaaaaaag catcgagcca catcgtccct ggcttttctt ttgaaaaacg
      541 ttcccgttca aaggctaaaa gactacctgt cgtatctata agaccagctt taagagatga
      601 ggtgccaata tcgattgaga gaactgttat aggactttgc ataggtacag agcctactct
      661 ttttttacaa gtttttcaat aatcgatcgg ttgtcgagga ggtcgctgag cgattggttg
      721 tgatgtattc gtataatcac atttgcaaaa agtccagaaa catttgagtt tatataccat
      781 tcgtatttga gaagttcttc atggtagaca gcatttgtcc cgatgatgcg gtaaaaaagg
      841 cctttttcgt aggcttcatt aaattgcgca atagcgtttc cgttaaaaaa gggtaggctg
      901 atggctgcga taacctttgt ggcgccttgt tcctttagaa attccatcgc ctttaaaagt
      961 gtgccacctg tgccaagcat gtcatcggcg agaaaggcaa cctttccttt tacatctccg
     1021 aggagtttta tagacacgat atttgttgtt tttgcatttt gcgtaacttg tgaataatct
     1081 cttactttat aaatcattgc aaggggtttt ttcaaacctg ttgagtaaaa cttatttcta
     1141 tcaaccgcac ctgtgtcggg agagacaacg acgaggtcat cgccgtcagc tgaaagattt
     1201 acaagtttcg taagttcacg aataatttga taacttgcgt gtaagttttc gataattgct
     1261 tttgaaaatg aattttctat ctctcgtgaa tgtatatcga gggtaatgat gcgggaaaca
     1321 cccatgtttt cgtatatatg tccgagtaaa cttgcggtta aaccctctct tccctttttt
     1381 ttatgctgac gactgtaggg gtagacgggg agcacaaggg aaatatcgct tgcgccagct
     1441 tggcgaacag cgtctatcgt aacaagcagg gacatcacat gatcgtttat agaaagaatc
     1501 tgcttgtttt tccccccgtt taaactgagt tcttgatggt tttctacatc ctgaaaaata
     1561 aatacatctc taccgcgaat gcattctttt atttcggttt tgaactcgcc gttcataaaa
     1621 taggtaaatt ctgtatcaac gaaaaatttt ggtgctcgat acttttttac atgccctctg
     1681 acgcataaat ctgaagtatg taagtcgttg tatagattta tatctcttac gaggtcatct
     1741 ttgtcagtat cgtatctttt tgaaataaca tcacgcttta gattgaagcg gtgcttgtac
     1801 atatggcgaa ggtgggtgat aacttcgttt gcaaagtgtt cgccccctgg acaggctaca
     1861 acggcaagct ttgttggctc tgagtatggc atccttaaaa cccctgggta attcaaacta
     1921 tgtgtttatg gtatcatttt acagagcttg ggtcaaggag aagattttcg tacaaaaaag
     1981 aaggtgcaat gaaggaactt ctgtcaataa aaaatagttt ttattgacag aagcatagtc
     2041 ctctgttacc ttatacagta tggatagacg acataattct ttaatgattg gcagtcttgt
     2101 taaaagactt gaaggtattt tgacgacaaa aagtaataat tttttaaaac agtataatat
     2161 tacttggtca caagtacaga ttctcaagta ccttgccgta caagaagagc tttgtcaaga
     2221 agatggtaaa gaaagagccg tttttcaaaa agatatagag atttttttta atttaacaaa
     2281 cccaatagta acgggtctca taaataggct tgaaacaaag ggcttgctcg tgcgtgaaac
     2341 cttatcgcga gatcgtcgtt ggagacgttt acgtataaca gaagaagggc ttgctcttga
     2401 aaagcatatt tacgaagagt ttattagaat ggacggcgat atgatggctc ctttttctga
     2461 ttcagaattg ggagatttta cactctatct agagcgactt ttacatagtc ttagtccaga
     2521 gatcttagaa aaaagtcgac aaaataaaaa tcttttgtaa atttttatct cactcccttt
     2581 taactaaggt cttttttttg aattagttag attctatcaa gcttttacca tagtcttctg
     2641 gagccttgta gccaagtaag tatcaaattc caaaatgtta aaaaaataaa tcggtctata
     2701 gtaaagatat tttaaatttg ctatactttg attaattaga aaaaagcaat gtaaatgtat
     2761 ttggtcttat atggcatacg tattgtttgg cggtgaggaa aaaagtgagt gttacaataa
     2821 aagatatagc tgctttagct ggggtttcaa aaacgtctgt ttcttttgcc tttaatagcc
     2881 cagagcgaat ttcagctgaa acatacgaga gaattatgac tattgcgcga gaacaaggat
     2941 actcgcctga tcctattgct cgaatcctaa gcaataaaaa gacaaaaaat ataggtattg
     3001 tacttccgca atctatatct gttttttttc aaaatccata tctcggagaa ttactacgtg
     3061 gtgttggttc tgtttgtgat caagaaggtt ttgctgtttc agttttatcc ccatttaaag
     3121 gaattgtttc acaaacaatt ttgaatgcag cagtagatgg aattcttatt atggggattt
     3181 caaaagacag tgaggtgcat acgaccttta ggcaacgaag catgccttat gttactatcg
     3241 atgcagcaga agtcggagat tttgtaaacg ttgggataag agatgtagaa ttagcaaagc
     3301 aacttatgga tattctcctt aaaaatggac atagaaaaat ctgtttttgt cttttgcagt
     3361 ctatttcatc aaatctccaa aaagcagata cttctggaac gagggaagct cgcttaaatg
     3421 gaatattgct ttctgcaaaa gaatatggct taaccgaaga agaaaaaaat gatttttatt
     3481 ttatgaatgt tccagctacc ttgtctgacg cttatgataa ggcactttct gttcttaaaa
     3541 agaaagagag acctactgct attttttgta tggctgatgt gcaagcctat ggtttttatc
     3601 gtgccgcgca agttcttggt ctttctattc ctgatgatct ctccatcgtt tctttcgatg
     3661 atttaccctt tactgaaaca ttggcgccgg gtttaacctg tgtgcatcaa agtgcgtata
     3721 aaaaagggcg cattgcaacg aagcttttat ttaaaatgat tgctgggcaa gaatgcgaat
     3781 ctgcaatttt ggaggcaaat ataaaacatc gaagctctgt ggcaaatatt agctaagaag
     3841 catgaatagc aaaaaaaaag cagatacttc aaaagtacct gctttttttt aatttctaat
     3901 atttctgccg tataacatac ttattttttt tagccactca gccttttctt tgttataatg
     3961 tgttttttcc atacgccatt gccagttaat accgccaatt gtcgaaggta gattcatacg
     4021 cgcttcgttg tttagcgcaa agatatcttg cagtggaata acagcaaaat tagctgtaga
     4081 gctaaaagcg agccgcacga gagattcgca tagttttcca ttttttgcaa agttttctat
     4141 atcattcttc tcaaaaccaa ggtattcccc aattaattgt atttcatctt ttgttgctgc
     4201 atctaaccaa ccttgcattg tcgcgttgtc gtgacttcct gtgtacacta cgcaattttt
     4261 atcataggta tggggtaaaa aagtgtttgt aaaaccacct tttcctgctt cgtttgtatc
     4321 aaaggcaaat tgtagaattt tcattcccgg taattcaaaa tcgtcacgta atttttttac
     4381 tgcatcggtt atgagtccta aatcttctgc tataactgga atatcaccaa gttcattttt
     4441 tatagctata aacaaatcat gattaggacc aggaatccat tttccattaa cagcagtttt
     4501 ttcgtccgcg ggaacgctcc agtaagcctc aaaaccccga aagtgatcaa tacggacaaa
     4561 atcaaccatc tttaataagg ctttaatacg atctaaccac catttatagc cttctttttt
     4621 aagtgcatcc caattatata agggattacc ccaaagttgc cctgtttcac taaaataatc
     4681 aggtggaacg ccagcaactg caagtggttt tccatcgtta gaaagttgaa ataaatgttg
     4741 gttcgcccat acatcagctg aatctgctgc aacaaaaata gggacatctc caataattga
     4801 aattccctca ttatttgcat attcttttag agcattccat tgtgtaaaaa agaaaaactg
     4861 tataactttt tgtatttcac attcaataac gtgggtttca tgccatttag aaacagcctt
     4921 tggatcatga gaggcaagtt ctttcggcca gtaattgctc cacattgctc cccaaatatt
     4981 ttcagtttta gcttttgcat catagacttc ttttatactc atgaaaatag cataattatc
     5041 aagccatgaa gcattatcgt ttttaaaagc ctcgtaatgt actcggtctt cgggttttgc
     5101 ccgatttaaa aattgctgag cagctgtttt taataaagga attttccaat taaccacttt
     5161 tccaaaatca acgcgagaac tgacgtttat gtcattcggt ggtattgttt gctcttcatc
     5221 aagccatcca tctctaaaaa gcatgtctat gtcgataatc aatggatttc cagcatatgt
     5281 tgaaaaagat tgatatgggg aatcaccata tccagtggga cttagtggca aaacttgcca
     5341 aagtgtttga ttcgcttcac ttagccaatc aacaaaagct cgcgcctcta agccaattgt
     5401 cccaataccg ggtgttccgg gcaaagatgt agggtgtaat aaaattccgc tcgctcggtt
     5461 catgttcata gttttatcct tttactgcgc cagcagcaat tcctttaata atgtattttt
     5521 gtaaaaataa ataaaacaaa ataatgggta atgaagaaat tgtgagagat gctgtcattg
     5581 caccataatc ttttaaaaac tgtccattga atctcatttg tgcaagttgg attgtacctt
     5641 ctcttaaaac aaggagtggg agaagaaaat cattccaaat ccaaagagcg tcaataactg
     5701 caattgttgc aatgattgtt ttaaggagcg gaaggacaat aacaaaaaag gtgtaaaatc
     5761 ttcctgctcc atcgattgct gcagattctt ccaattctcg tgggacgctt tttacaaagc
     5821 catgaaacat aaatatagct gtgggacaac caagtcccca gtacataagt ataatgccag
     5881 gtgtatttgt aaggttaaag tctgatgcca agacagctat tggtaccata ataatttgaa
     5941 aaggtatgac caaggcaaag gtaaaaatag aataaattat ccatgacgtt tttgtagtgg
     6001 ttcgagcaag catatatgca cacatcgaac ttgttagaat tatgccgaca actccggctg
     6061 ttgtaataaa aaaggtgttt aaaaacactt ttggaaattc catttcattc caagctttaa
     6121 tatagttacc catataaaag ctattaggaa gaccagttgg aaaacgcgtt atatcagcac
     6181 tttccctcaa tgaatttata attacgtaga tcattgggta aaagaaaaaa attgaaaaaa
     6241 taaggactat accaaatact actttcgttg ttgggcttgt atgttttctt tttaaagttg
     6301 ccattattgt tctacctccc gttttttcat aacctgtagt tgtaagcctg ttataagtag
     6361 gataatcaag aaaagtaaca aggcctttga agttgccatt cctgcgagtt tgtgtgaaaa
     6421 ggcttcatag taaatatcaa gtacaaatgg aactgtgctg gttgcataac ctgatggccc
     6481 aaccattgca tatatgagat caaagctttt aagggcattt gctattgtta aaaagagtga
     6541 aatagtaata gcgggaataa taagaggtat ggttataaac cggaatcgtt gaaaaccact
     6601 tgctccgtcg attaaagagg cttcaataac atcggttgga atattctgta agccagcaag
     6661 gtagacaatc atataatatc cacagccctg ccaagtagct actagtataa gaagccatgg
     6721 tgccttactt gcatcgctga gccaaagatc tatgccactt atcgctggta ggatttgata
     6781 gaaaagcatt ttccaaatga gcgctacgat aatcattgaa aggacgtttg gtaaaaagaa
     6841 aattgttcta aatatttttt gccctggcaa gtctgagtct aagacaaggg caagtaaaaa
     6901 ggctaaaacg ttgattccta taacagaaaa tagagcaaaa aagaaggtaa agccaactac
     6961 accgagttgt attttatctg cagtccatat tgtcgagagt atgtttctaa aagttccaag
     7021 gttaaaaatt tcgtcggttg tttcgtgtat aatagtttca cttgcctgag aaaaaaggtt
     7081 tgcttttaaa aatgcgtcta cgctgttttc ttttgctttg ttatcttctg ccaaagaaat
     7141 atcgtttatt tttctcaggt agacatctat atcagagtcg cttacagagc cgagagagta
     7201 ttcgggaaga gaccatatct tttcttctat ataaaatgta ttatattcat tttttaatat
     7261 ataaaatgta tcttcaagtt cgtaatgaag taaaaaaaga gctttttctt catcagaagc
     7321 tttttttaaa atttgttttg tcgctttttt ttcttcaatc cgtagaggta atgaagagct
     7381 tgcgttgtaa tatatttctg ttttttttct tggataaaag gtatccttta cctttcctga
     7441 aagaatatct ttgtagtttt ttaagccaac aaagctgttt ttttcgggtg aatatttaac
     7501 agactttaaa attctttctg ttttgtttcg ttccgctcca ccgatgttta atcgactata
     7561 ggtatgagca ttttcgtcga gtgtatagac agataagaga aaatctttat ctgattgttt
     7621 ttttatttta tctagaatgt ttgtttcaaa ttcagtttta tctaaactga tgggggactt
     7681 tggtgtcaat ccatcccagt ttgtcattga aatcgaaatt cctttaaaaa atggatagat
     7741 aaagaaaaac gtgtataata ctacgcatgg taaaataaag gctatgaagt atccatattt
     7801 ttttgttttg ggttctacca tttaatgtcc ccttgtataa ttgtaatgaa aggctgttta
     7861 caaaagtaaa cagccccttg aaagtgtaag ataagtcagc ttattttgct gctttccatg
     7921 ttgcatcgat gttttttatt acatcgtttg ctgttgttcg tttaagcatg tattgttgtg
     7981 caccattttt gcatgcttcc tcaaatacaa cgcttgggta cattgagaaa agccatggat
     8041 atgaaccctg cgcagatacg cctttaataa ga
//
LOCUS       DAOSSW010000078         7889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_8662_length_7889_cov_5.916390, whole genome shotgun sequence.
ACCESSION   DAOSSW010000078 DAOSSW010000000
VERSION     DAOSSW010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8662_length_7889_cov_5.916390"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2198)
                     /gene="rpoB"
                     /locus_tag="PKW26_06820"
     CDS             complement(<1..2198)
                     /gene="rpoB"
                     /locus_tag="PKW26_06820"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOQ93315.1"
                     /translation="MFARSRTINRQYIGKNIQSFMDLPDLVDIQLKSYEGFLQKNTLD
                     NHEPLAEQGLEDVFRSSFPIESPNGDMLLEYEFYEFDNEAIKYTEFECKQKGLTYSVP
                     LKARINLIFTQTGEIRQKDIYMGDIPLMTDRGTFIINGAERVVVSQIHRSPGVIFSHE
                     KGIFSTRIIPYRGSWLEFEVDQKKELIYAKIDRKKRILGTIFLRALGISSREEIIKAF
                     YTTEKIAVVDDRDSKESLIGKVLAVAVSIKDADGEEERVLYKAGEKLHPHNIDELVLH
                     GVSEIEAIAFDAENSLNSQIIINCFEREEIRFAKEGAASEEPTQEDALSAVYSVLMPG
                     EPITLESAEKDLSTMFFSLRRYDLGRVGRYKLNKKFNYAEPLEDQALTLQDILEAMKF
                     LIKVYVGDEEIDDIDHLGNRRVRSVGELLTNQLKKAFSRMERIAKERMTSKETETIKP
                     QDLISIKPIGASIKEFFGSSQLSQFMDQVNPLAELTHKRRLNALGPGGLSRDRATFEV
                     RDVHYTHYGRMCPIETPEGPNIRLIVSLAVYTRVNDYGFLEAPYRKIVDGKAVDQIDY
                     LSAMDEDRYYIAQISEKIDEKGNFLTEQVPCRRYGDYTSRSPKDVQYMDVSPKQIISV
                     SAALIPFLEHDDANRALMGSNMQRQAVPLLFPEPPRVGTGMEAKCAVDSGVLVKARRA
                     GEVVWVSSDMIKVKPTDAKDKDELDEYQLLKYQRTNQDTCYHQRPLVEVG"
     gene            complement(2328..2708)
                     /gene="rplL"
                     /locus_tag="PKW26_06825"
     CDS             complement(2328..2708)
                     /gene="rplL"
                     /locus_tag="PKW26_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOQ93316.1"
                     /translation="MAALTTDQILEAIASMSVLEASELVKAMEEKFGVTAAAPVMVAG
                     AGAGPAEAAEEKTEFTVTLESADATKKIAVIKAVREVTQLGLKEAKDLVEGAPQTLKE
                     DVSKEEAAKIKETIEAAGGVVKIA"
     gene            complement(2803..3315)
                     /gene="rplJ"
                     /locus_tag="PKW26_06830"
     CDS             complement(2803..3315)
                     /gene="rplJ"
                     /locus_tag="PKW26_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOQ93317.1"
                     /translation="MSDVKKIQKYKLEAVESTKNLFETYSDYIFTNYRGLTVQQLTEL
                     RRQLAKKNAELRVVRNNLARIAFEQMGVDSLGDYFTGPTAIALGNEDANEVAKILFDY
                     AKNAPALEVKGAFLDKEIYDAEKIEAFSKLPGKSQLIAMIMSTINAPVQKLAATLQAY
                     VDKKQEEGEA"
     gene            complement(3317..3997)
                     /gene="rplA"
                     /locus_tag="PKW26_06835"
     CDS             complement(3317..3997)
                     /gene="rplA"
                     /locus_tag="PKW26_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOQ93318.1"
                     /translation="MKRGKKYQEAIKKHDLQKSYDVLQACEIVKDIKFAKFDETVELH
                     VNLKLTKSQFVRDTLVLPHQFKGEKRVLVFCKEDRVKEALDSGATFAGSDEYIEKIRG
                     GWLDFDIAVATPDMMKDVGRLGMVLGRRGLMPNPKTGTVTNDIAAAIDELKKGRVEYR
                     ADKSGVVHIAIGKVSMDADKIADNAQALMSELSRKKPADAKADYVQSVSVSSTMGPGV
                     WVNYAVGE"
     gene            complement(3999..4430)
                     /gene="rplK"
                     /locus_tag="PKW26_06840"
     CDS             complement(3999..4430)
                     /gene="rplK"
                     /locus_tag="PKW26_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOQ93319.1"
                     /translation="MAKKKVTAVIKLQCPAGKATPAPPIGPALGPHGVSAPQFVQQFN
                     DRTKAMEPGLIIPVVLTVYQDKSYTFILKTPPAAVLVKKAISLAKGSDNPLKTKVGKL
                     SQASLEEIAKTKLPDLNANDIEAAKKIIAGTARSMGVEVER"
     gene            complement(4576..5130)
                     /gene="nusG"
                     /locus_tag="PKW26_06845"
     CDS             complement(4576..5130)
                     /gene="nusG"
                     /locus_tag="PKW26_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOQ93320.1"
                     /translation="MGRSWYILQTYSGYENKIEKAIRSLMEKGDLSSSVFYDVKVPVE
                     EVTEVKDGKKKTTTRKFLPGYIMVEMDLPDLGWKNVCTTIRRIQGVSGFIGAGSNEKP
                     RPLSADEAKHLLQKSGQLKGDKPRMKQSFMVGEQVKIIDGPFASFTGTIEEVNQEKNK
                     LRVMVGIFGRATPVEVDLLQVEKE"
     gene            complement(5140..5319)
                     /gene="secE"
                     /locus_tag="PKW26_06850"
     CDS             complement(5140..5319)
                     /gene="secE"
                     /locus_tag="PKW26_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOQ93321.1"
                     /translation="MGKVRSFLKESIAELKKVVWPSREDVISSVKIVLISTTVIALIL
                     GLLDLLFVSGMNYIF"
     gene            complement(5348..5420)
                     /locus_tag="PKW26_06855"
     tRNA            complement(5348..5420)
                     /locus_tag="PKW26_06855"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5385..5387),aa:Trp,seq:cca)
     gene            complement(5467..5637)
                     /gene="rpmG"
                     /locus_tag="PKW26_06860"
     CDS             complement(5467..5637)
                     /gene="rpmG"
                     /locus_tag="PKW26_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOQ93322.1"
                     /translation="MAKKTSVEIVALQCSECKRRNYTTYKNRKNIQGKLEFNKYCPFD
                     QKHTVHKETKAK"
     gene            complement(5771..5843)
                     /locus_tag="PKW26_06865"
     tRNA            complement(5771..5843)
                     /locus_tag="PKW26_06865"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5808..5810),aa:Thr,seq:ggt)
     gene            5972..6859
                     /locus_tag="PKW26_06870"
     CDS             5972..6859
                     /locus_tag="PKW26_06870"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOQ93323.1"
                     /translation="MKSIDTLLTEILDSYEQVGGINLDGVNQFPNREAVIRLLKDLQA
                     LVFPGFLTAETLDSANLRHLTGERIHRIVASLTQETKKALLYVCDASCVNANTCLHLA
                     QNTSLALIEEIPEIRRKINLDAKAAMEGDPAAKSVEEVILSYPGLEAILVHRIAHFLC
                     ENGVPIIPRIMSEYIHGKTGIDIHPGAKIGESFFIDHGTGLVIGETTIIGNNVKLYQG
                     VTLGALSVKKEMADKKRHPTIEDRVTVYPGATILGGDTIIGAGSVIGGNVWVTKSVPP
                     NSTVRYFQNGDTNSQKTRL"
     gene            7092..7457
                     /locus_tag="PKW26_06875"
     CDS             7092..7457
                     /locus_tag="PKW26_06875"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOQ93324.1"
                     /translation="MKIKRIDYCILILALTLCVFSFYMISSQKGQATQLVIESPEAVW
                     KYPLEKDRIIKVQGSLGESIIEIQDKKAFFVDSACPNKTCVLSQPIEKKADWSACLPN
                     RIFIRIDGSDESGLDAISF"
     gene            7493..>7889
                     /locus_tag="PKW26_06880"
     CDS             7493..>7889
                     /locus_tag="PKW26_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOQ93325.1"
                     /translation="MAKKNADALVYICTQCNNTQPKWLGRCPECGEWNSFVEKRANEA
                     FFSTTKAHAIPLKALSSEDGKRYQTGIAEFDIVLGGGLVKRSCSLIGGEPGIGKSTLL
                     LQVSARFPGKVLYVSGEESASQIKSRANRL"
BASE COUNT         2272 a         1648 c         1488 g         2481 t
ORIGIN      
        1 tggccaactt ccacaagagg tctttggtga taacaggtat cttgattggt tctttggtat
       61 tttaagagtt gatactcatc aagttcgtcc ttgtctttcg catctgtggg tttgactttt
      121 atcatatctg aagaaaccca tacgacttca cctgcgcgtc ttgcctttac taaaacacca
      181 gaatcgactg cgcattttgc ttccataccc gttccaacac gaggtggttc tgggaaaaga
      241 aggggaactg cttgacgttg catgtttgaa cccatgagcg cacggtttgc atcgtcgtgt
      301 tccaaaaagg gtataagagc cgcagaaacc gaaataattt gcttcggaga cacgtccatg
      361 tattgtacgt ctttcgggct tcgtgatgta tagtccccat aacgtcgaca gggcacctgt
      421 tcggtcaaaa aattgccctt ttcatctatt ttttctgaaa tttgagcaat atagtatcta
      481 tcttcatcca ttgcggaaag gtagtctatt tggtcaactg cttttccatc aacaatcttt
      541 ctatacggtg cttccaaaaa gccgtaatcg ttaacgcgag tatacacagc gagtgaaaca
      601 atgagtctga tatttggtcc ttcaggagtt tcaatcgggc acattcgacc atagtgggtg
      661 tagtgaacgt cacgaacttc aaaggttgct cggtcacggg acaaaccgcc aggaccgaga
      721 gcatttaaac gtcgcttatg agttaactca gcaagcgggt ttacttgatc catgaactgg
      781 gaaagttgac ttgagccaaa aaactcctta atcgatgcac cgattggttt aatcgaaatt
      841 aagtcttgtg gtttgattgt ctcggtttct ttagaagtca ttctttcttt tgcaatgcgc
      901 tccattcgac taaaggcttt ctttaactga ttcgtaagaa gctcgccaac agatcgcaca
      961 cgtcgatttc ccaagtggtc gatgtcgtca atttcttcat cgccaacata aaccttaatt
     1021 aaaaacttca tcgcctctaa aatatcttgc agtgttaaag cttgatcttc tagcggctct
     1081 gcgtagttaa actttttatt gagcttatag cgccctactc gaccaaggtc atagcgacgc
     1141 aaggagaaaa acatggtgct taaatctttt tcagcacttt ccaaagtaat gggctcgcca
     1201 ggcataagca cagagtagac agctgacaaa gcatcttctt gcgtaggttc ttcagaagct
     1261 gcgccttctt ttgcaaagcg aatttcttca cgctcaaaac aattgataat gatttgagag
     1321 ttaagagaat tttctgcgtc aaaagcaatc gcttctatct cagaaactcc atgtaaaaca
     1381 agctcgtcaa tattgtgcgg gtgtagtttt tcgccagcct tgtataagac tcgttcttct
     1441 tcaccgtcgg catctttgat agaaaccgca acggcaagaa ccttcccaat gaggctttct
     1501 ttactgtcgc gatcatcgac aacggcaatt ttttctgttg tgtaaaatgc tttgattatt
     1561 tcttctcgag agcttatacc aagcgctcgc aagaaaatag taccgaggat gcgttttttt
     1621 cggtcaattt ttgcatagat aagttctttt ttttggtcaa cttcgaactc aagccaagaa
     1681 cctctatagg ggataattct tgtagaaaaa attccctttt catgagaaaa aataacacca
     1741 ggagatctat ggatttgcga aacaacaacg cgttccgctc catttataat aaaagttccc
     1801 ctgtcggtca tgaggggaat atctcccata taaatatctt tttgacggat ttctcccgtc
     1861 tgagtaaata tcaaatttat gcgtgctttt agaggcacag aataggttaa acctttttgc
     1921 ttgcattcaa attctgtata ttttatagct tcattatcga attcataaaa ttcatactca
     1981 agcaacatgt cgccatttgg actttcaata gggaatgatg acctaaaaac atcttctaat
     2041 ccctgttctg caagtggttc atggttatcc agtgtattct tttgcaaaaa accttcatat
     2101 gattttagct gaatatcaac aagatcgggt agatccataa aactctggat attctttcca
     2161 atatactgac gatttatcgt ccgacttctt gcgaacatca aatccccctt aactttaaca
     2221 aagtatcata atttcatttt tatacaaaca gagctaccta agaaattctt aggcagcttg
     2281 tttatcagcc atgtgagagc acgtttttat tataataata aaaatactta tgcaatcttg
     2341 acaacaccac cagcggcttc gattgtttct ttaatttttg ctgcttcttc ttttgaaacg
     2401 tcttctttaa gtgtttgagg tgcaccttca acgaggtctt ttgcttcttt aaggccgagt
     2461 tgtgttactt cacgaactgc cttaattaca gcaatttttt tggttgcatc agcgctttct
     2521 aaagttacag taaattctgt tttttcttca gctgcttctg ctggtcctgc acctgcacct
     2581 gctaccatta ctggtgctgc tgcggttacg ccgaattttt cttccattgc ttttacgagc
     2641 tcggaagctt cgagtactga catagaagca attgcttcta aaatttgatc tgttgtgaga
     2701 gctgccatat tatcttctcc ttataatata attttttatc actgcgacag gacgacagct
     2761 aagaagcctg acgcattttt ggaagccatc tgttagccag aattacgctt caccctcttc
     2821 ttgtttttta tctacatatg cctgcaatgt agcagcaagt ttttgaactg gcgcgttgat
     2881 tgtagacata atcatagcaa taagttgtga tttgccaggt agttttgaga atgcttcgat
     2941 tttttctgca tcgtatattt ctttgtctaa gaaagcaccc tttacttcta aagcaggcgc
     3001 attttttgca tagtcaaata agatttttgc aacttcgttc gcatcttcat ttcccaaggc
     3061 aatagcagtt ggtcctgtaa aataatctcc aagagaatcc acacccattt gctcaaaggc
     3121 aatacgagcg agattgtttc gaacaacacg taattctgca ttcttttttg caagttgtcg
     3181 tcgtaactcg gtcaattgtt gcaccgttaa accacgatag tttgtaaaaa tataatcaga
     3241 ataggtttca aacaagttct tggtagactc tacagcttcc agtttgtatt tttgtatttt
     3301 tttaacatct gacatcttat tctcctactg cgtagttaac ccatacgcca ggtcccatag
     3361 tagaactcac agaaactgac tgtacataat cagcttttgc atcagcaggt ttttttcttg
     3421 aaagctcgct catgagggct tgcgcgttat cggcaatttt atcagcatcc atggacacct
     3481 ttccaattgc aatgtgtaca acgccgctct tgtcggcacg gtactcaacg cgaccctttt
     3541 tcaactcatc gattgccgca gcaatgtcgt ttgtcactgt gccggttttt gggtttggca
     3601 tcaaaccacg gcgaccaagc accattccca agcgcccaac atctttcatc atgtcgggag
     3661 ttgcaacagc aatgtcaaaa tcgagccaac caccgcgaat tttttcaatg tactcatctg
     3721 aacctgcaaa ggttgcacct gagtctaaag cttcttttac tctgtcttct ttacagaaca
     3781 caagcactcg tttttcgcct ttaaattgat gtggtaaaac caaggtatct cgtacgaatt
     3841 ggcttttggt caattttaga tttacgtgta actctaccgt ttcatcaaac tttgcaaatt
     3901 taatatcttt tacaatttca caagcttgca aaacatcata ggatttttgt aaatcatgct
     3961 tttttatagc ttcttgatac ttttttccac gtttcatatt agcgctccac ctcaacaccc
     4021 atactgcgtg cagtaccagc aataatcttc ttggcagctt ctatgtcatt tgcgtttaag
     4081 tcaggaagtt ttgtttttgc aatttcttcc aagctcgctt gtgacaactt accaaccttt
     4141 gttttcagag gattatctga acccttagca agactgatag ctttttttac taaaacagct
     4201 gccggtggtg ttttaagaat aaaagtataa ctcttatctt gatagacagt gaggacaaca
     4261 ggaataatca aaccaggctc catagccttt gttcgatcat tgaattgttg aacaaactga
     4321 ggtgcactta cgccgtgtgg accaagcgca ggaccgattg gtggggctgg agttgccttt
     4381 cccgcagggc actgtagctt gataacggcg gttaccttct tctttgccat atttttctcc
     4441 ttcttggtac gcagaatggc ttctgctaac gaaaccgaaa tttattcaac gaataaaaac
     4501 cgacttctcc caaatatttt gcttagataa aaaaacattt tcttatccaa atattctata
     4561 ctttatttta agaaactact ctttttctac ttgcaataaa tcaacttcaa caggagttgc
     4621 ccgtccgaaa ataccaacca ttacgcgtag tttgtttttt tcttgattta cttcttcgat
     4681 agttccagta aacgatgcaa aaggaccgtc gataatcttc acttgctcac caaccataaa
     4741 actttgtttc atgcgtggct tatctccctt taactggcca gatttttgca ataagtgctt
     4801 tgcctcatcg gcagataagg ggcgaggttt ctcattactt ccagcaccaa taaaaccaga
     4861 aacaccttga atccttctaa tggttgtaca aacgttcttc caaccaagat ctggcaagtc
     4921 catttccacc ataatatagc cgggaagaaa cttccttgtc gttgtctttt tcttaccgtc
     4981 ttttacttct gtaacttcct ctacaggaac ctttacatcg taaaaaacag atgacgacaa
     5041 gtcacctttt tccatcagag aacgtattgc tttttcgatt ttattctcat agcctgaata
     5101 ggtttggaga atataccagg atcttcccat tatagcacct taaaaaatat aattcatgcc
     5161 agaaacaaaa agaagatcta acaaacctag aataagtgca atcacagtag ttgatataag
     5221 cacaatcttc acagaagaaa taacatcttc tctacttggc catacgactt ttttcaactc
     5281 tgctatagat tcttttaaaa aacttcttac ttttcccatt attaaaactc ctaaacaata
     5341 cgcttttcag gccaggaagg actcgaaccc tcaacattcg gttttggaga ccgacgctct
     5401 accattggag ctactgacct atttatatta taaaccttta taataaaggc tctaatcata
     5461 caaaacttac tttgcctttg tttctttgtg cacagtatgc ttttgatcga agggacaata
     5521 cttattgaac tcaagtttcc cttgaatgtt ttttctattc ttgtaggttg tataattcct
     5581 tcgcttgcac tcgctacatt gcagtgcaac tatttctaca gatgtttttt ttgccattat
     5641 cttacccctt atgtaaaaaa acaagcttct aaaagccttt taggcaagtc taaaaataat
     5701 cctcgagatg cctagataca ttttatatta atatctttaa tttttgaaag tcaaagcctt
     5761 cattattaag agcctccgag cgggatcgaa ccgctgacct caaccttacc atggttgcgc
     5821 tctgccgact gagctacaga ggcacaaaaa tccatcacac aaatcgtgca aatgaaaaga
     5881 gaagttctag tactatagtg ataaaacaga aacttgtcaa gccaattaag gaagggtttg
     5941 actaaaaagt caaaacgagt atagtaaacg tatgaaatca atcgatactt tattgacaga
     6001 aatattagac tcctatgagc aagttggcgg tataaacctt gatggggtaa accagttccc
     6061 aaacagagaa gctgtgatac ggcttttaaa agaccttcaa gcccttgttt ttcctgggtt
     6121 tttaaccgca gaaactcttg actcagcaaa tctgcgccat cttactgggg aaagaattca
     6181 cagaattgtt gcttctctga cgcaagaaac aaaaaaggcc cttctctatg tttgcgatgc
     6241 aagctgcgta aacgcaaata cctgtctgca tcttgcacaa aatacctctc ttgctttaat
     6301 agaagaaata cctgaaattc gacgaaaaat aaatctcgat gccaaggcag ctatggaggg
     6361 tgaccccgca gcaaaaagcg tcgaagaggt tattttatct tatcctggtt tagaagcaat
     6421 tctcgtgcat cgcattgcgc attttctatg cgaaaatggg gtgcctatta ttccaagaat
     6481 tatgagtgaa tacatacacg ggaaaaccgg gatagatatc caccccggag caaagatagg
     6541 cgagtctttt tttattgacc atggaacggg gcttgttatc ggggaaacaa caataatcgg
     6601 caataatgta aagctctatc aaggagtaac gctcggtgcc ttaagtgtaa aaaaagaaat
     6661 ggcagataaa aaaagacatc caaccattga agatcgggta acggtttatc caggagctac
     6721 aattctcgga ggggatacta ttatcggggc aggttcagtc atcggtggaa atgtatgggt
     6781 aacaaaatct gtcccgccaa attctacggt gcggtatttc caaaatggtg atacaaattc
     6841 acaaaaaaca cgtctctaaa caataaaaca agttaatttg gggaaggaat aaaaaaaagg
     6901 gctcttaagt aaccgacggt tagttaagag cccccgtcca gcaagccgga catcggagag
     6961 agtttatcag cttatttaca agctgagtta aatgtaacac gcattggaat ttgtgtcaat
     7021 agtttttaga aattaatttt atatttttaa agattttgat ttttttgaaa aaatgagtac
     7081 aatatcatat aatgaaaata aaacgaatcg attactgcat acttattctc gcccttacgc
     7141 tttgtgtttt ttctttttac atgatttcat cacaaaaagg acaagctacg cagcttgtta
     7201 ttgagtcgcc tgaggctgtt tggaaatacc ctctcgagaa ggaccgtatt ataaaggtgc
     7261 agggcagttt gggtgaatct attatagaaa tacaggataa aaaagctttt tttgtggatt
     7321 ccgcttgtcc aaataaaact tgcgttcttt cacagcccat cgaaaaaaag gctgactggt
     7381 cggcatgttt accaaatcgt atttttatcc gtatcgatgg aagtgatgaa agtggtcttg
     7441 atgcgataag tttttaataa atagtagttt tattcgtttt aaaggggttt tgatggcaaa
     7501 gaaaaacgca gatgcgcttg tttatatctg tacgcagtgt aataatacac aaccaaaatg
     7561 gcttgggcgt tgtccagagt gcggtgaatg gaatagtttt gtcgaaaaaa gggcaaatga
     7621 ggcttttttt tctacaacaa aggcacacgc aatacctcta aaggcgcttt cgagtgagga
     7681 tggaaaacgc tatcaaacgg gaatagcgga gtttgatata gttttgggag ggggtttggt
     7741 aaaacgttcc tgctcactca taggaggcga gcctggcata gggaagtcga cgctcttgct
     7801 acaagtaagt gcgcgttttc cgggaaaggt gctctatgtg agtggggaag aatctgcaag
     7861 tcaaataaaa agccgcgcaa ataggcttt
//
LOCUS       DAOSSW010000079         7804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_8786_length_7804_cov_6.372822, whole genome shotgun sequence.
ACCESSION   DAOSSW010000079 DAOSSW010000000
VERSION     DAOSSW010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8786_length_7804_cov_6.372822"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKW26_06885"
     CDS             <1..180
                     /locus_tag="PKW26_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93326.1"
                     /translation="NATNKGKREGFDAGLARGRNEGLEQGRAQEKIQTARSLKARNFS
                     TEDILEITGLSEDEY"
     gene            complement(367..1221)
                     /locus_tag="PKW26_06890"
     CDS             complement(367..1221)
                     /locus_tag="PKW26_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOQ93327.1"
                     /translation="MANEVVFKKQKKNEIKQSIKEIKERAKQDILVLKKELTIELAAD
                     KTDALRKIQARDEKKRLYQEKKAAYYAGPKRYSVMEEIWNSITHGIGVGLSIAALVIL
                     LVRAVALAPQAEMTRFLVGYSIFGSSLIILYLMSTLYHALTPYGAKKVFAIFDHSSIY
                     LLIAGTYTPFCLTVLHGALGWTLFGIIWGLAILGVVFYAVFGNRMRALSAITYVLMGW
                     LIIFAFGPMKAAAPAESIIYLLVGGAAYTLGVIVYALKKIPHTHSIWHLFVLAGSIFH
                     FFSLYVLL"
     gene            complement(1260..2048)
                     /gene="rlmB"
                     /locus_tag="PKW26_06895"
     CDS             complement(1260..2048)
                     /gene="rlmB"
                     /locus_tag="PKW26_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOQ93328.1"
                     /translation="MKKTITGFHAIEERLRFYKSKNLEKSTAIRYTLYFDTRGPRIKK
                     ILSEATKLNIQSKQVSKAELNQLVSGLSDFAREHRGLVLIIEGEKDEKKERVDLTAFL
                     STLSVSKEEKEESIVVVLDSISDPQNVGAILRSADQFGVDLVIAPKRESALESEIIDR
                     TSSGASAWVAMAVVPNLVRAVEELKKAGYWIYGADAGGKNLKEIAFEKKVCLIMGSEG
                     KGISRLLKENCDEIVSIPTEGKLDSLNVSVAAGILLYEINSSAL"
     gene            2313..3587
                     /gene="pepT"
                     /locus_tag="PKW26_06900"
     CDS             2313..3587
                     /gene="pepT"
                     /locus_tag="PKW26_06900"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOQ93329.1"
                     /translation="MAKNRIQEALAKTDLKEALLKRFISYVQIDTQSNAAEADAGSIP
                     STEGQKELARLLEAELKELGFDELFVSEHFYVCARLNATKAKEKTPSLCFLAHMDTSD
                     AVSGKDVQVKISENYDGRAIVLGSGPEGLALDPELEPELLDCLGDSIIHTDGKTLLGA
                     DDKAGIAEIITALAYIIEENIPHGQIELIFSPDEETGHGMDKVPFDWIESEFCYTLDG
                     SHLGEVEYECFNAYKSDIRFIGKAAHTGTARGKLINALTMAAFYIGLLPRNESPETTD
                     GYEGFFTAMDLSGNIEEANLTVFIRDFETESMQNRLKRLEIFAEATEAHFPGSKVEIQ
                     HTEQYKNMKDALLKKPEAMQLVKKALDELDIEMISKPIRGGTDGSRLTEMGKPTPNIF
                     TGGHSFHSRTEWASLSQMIWATETIIRIIKNA"
     gene            3623..4906
                     /gene="murA"
                     /locus_tag="PKW26_06905"
     CDS             3623..4906
                     /gene="murA"
                     /locus_tag="PKW26_06905"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOQ93330.1"
                     /translation="MYEYCIEGGFPIKGTIKASGNKNAALPCIAASLLSSEPVILHNI
                     PEIEDAGVMLDVLKELGASVENLGNNSWKIHAKDIHTHTIPLESAKKIRASILFAGPL
                     LARLGKVALPPPGGDVIGRRRLDTHFLALTELGARVEMDRQFTFSANKLVGEDIFLDE
                     ASVTATENAIMAASLATGKTILSNAASEPHVQDLCKMLNAMGAKISGIGSNILTIDGV
                     ESLGGCEYKIGPDFMEIGSFIGLAAATKGSITITDIEPRDLRPIKLAFAKLGIHWEIN
                     GTNLLVPTGQEMRVNPDLGGMIPKIDDAPWPGFPPDLTSIMTVVATQVEGTVLIHEKM
                     FESRMFFVDKLIAMGARITLCDPHRAVVSGPSTLHGSELISPDVRAGMAMIIAAMSAR
                     GESVIKNVYQVERGYENLTERLKSLGARIERRSSC"
     gene            complement(4971..6797)
                     /locus_tag="PKW26_06910"
     CDS             complement(4971..6797)
                     /locus_tag="PKW26_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93331.1"
                     /translation="MKIYKHVIAVILIYLLFSFVGIGLFVFFTSPVPEEFASYATQYK
                     IYEALLVFLKFLPSLLLSAFLVGLSWSFGRSDSEKVERFSTIRVAFFKNVIALSFTCI
                     VLCMLSSEVFIPLLQNTQENMENKEKNFEAYLLLAAESAYADDYIYAKYYIDNALALK
                     PNAVEALDLKDTIDIFYSGMKQKIRDREMQEQVSSPISVQDYGYTSPQLMERAREAFL
                     KEDFFNAHYYANLLLEMTEKKDGNRQEAQQLAADAWNKLQDNPTGLDGEQVLAYETKR
                     KGYVAFINQEFEKAYYIFSDLKTAYPADLDIDRYFLLSKEKMDGKYFFSNEVEDLINF
                     EQYKNVYFSVKKPEGGFFLLYWKGGTAKEKAGNLIQYLRELNVFSYDENKELEFSFSV
                     PYAKLVAFPLEQTPQTFQTDMYKRFKIKKESSQFVPLIYLVSTDSEKDGLYLGPSFSY
                     EREVGSNHYNSLLLAMPFDDFLQICKASLGVKVMPLTVLASFAKKADSYGFSKEVYFQ
                     TFMDRMSYPFVLLIVLILLAGISWNYKLGAETHFKFVWLITLPLFTFILYFVLETILY
                     LLNLFSFVIIGIFGGVAPWVYLLILCVLVLLSAIIFSALRSD"
     gene            complement(6805..7746)
                     /locus_tag="PKW26_06915"
     CDS             complement(6805..7746)
                     /locus_tag="PKW26_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93332.1"
                     /translation="MKNAFFMVFVFIILTIATAFVSTVAISLIHLSFSMVAGENLSFV
                     NFALFFQVLIKALPFCLVFACLGLIFYFIRHPNISILSTLFYFIIGFSIWLFAIPWML
                     SMQLAQKPLVSEYEARRLSEGFFRQTKSSVDYIARFFPEGGAEGVRIDTSSSGHIESV
                     FFDEDVQVPEQAFYADPLFEKTIDLPPVLEVLKTFFYFARTEAIKSLARGYLSYLMFA
                     SFGLALLSIFFVRNINSWKLINVSLVVALFVSICKINREVFSAVLFDKMIGFFQHNNM
                     LWFTNRNNIQFVINISITLCFCLIGLVHFIRQRQKIK"
BASE COUNT         2480 a         1604 c         1554 g         2166 t
ORIGIN      
        1 aatgcaacaa ataagggcaa gagagaaggc tttgacgcag gacttgcacg aggcagaaac
       61 gaaggcttag aacaaggtcg agcacaagaa aaaatacaaa ccgcccgtag tttaaaggct
      121 cgcaattttt ccacagaaga tattctcgaa ataactggtc tctccgaaga cgagtactag
      181 ttttaaactc tccccaaagc gactgaaata ctccctccgc gacatggagg tcgcgcacaa
      241 tcagcacgac agtttaggtt tttcctaaac tgtcaaaata attttttaca cggatgtaaa
      301 aaattatgta tcatgttaat cctgtataca caattagttt tccaagcaaa ccagaacgaa
      361 ttttttttac aaaagtacat ataacgagaa aaagtgaaag atacttcctg caaggacaaa
      421 taaatgccaa atactatgtg tatgggggat ttttttgagg gcgtagacaa tgactccgag
      481 ggtataggct gcgcctccga cgaggagata aatgatgctt tctgctgggg cggcggcttt
      541 catggggcca aaggcaaaga taatgagcca gcccatgagg acgtaggtta tggcagagag
      601 tgctcgcatt ctatttccaa agacggcata gaaaacaact cccaagattg ccagtcccca
      661 gattatgcca aatagggtcc agccaagggc gccgtggagg acggtgaggc aaaagggggt
      721 gtaggtgcct gcgatgagta gatatatcga tgaatggtca aatatcgcaa agactttttt
      781 tgcgccgtaa ggtgtgagtg catggtagag ggtcgacatg agatacaaga ttatcaatga
      841 tgagccaaag atgctatagc cgacgagaaa tcgagtcatt tctgcctgtg gtgcaagggc
      901 gacagcgcgt acgaggagga taacgagggc agctatggac aaacctactc ctatgccatg
      961 ggttatggag ttccatatct cttccataac agaatatcgc ttgggaccag cataataggc
     1021 agcttttttt tcttgataga gccgtttttt ctcatccctt gcctgtattt ttcgtagtgc
     1081 atctgtttta tcggcagcaa gttctattgt aagctctttt tttagtacaa gaatatcttg
     1141 ctttgcacgc tcttttattt ccttaatgct ttgctttatt tcattttttt tttgtttttt
     1201 aaatactact tcatttgcca tattttcctc caaaattgtg tttataagcc cttgtttttc
     1261 tacaaggctg agctatttat ttcatacaat aaaatacctg ccgcaaccga gacgttgagg
     1321 ctatcgagct tgccctctgt tggtatggaa acaatttcat cgcaattttc ttttaaaagc
     1381 cgagaaatgc ccttcccttc gcttcccatt atgaggcaga cttttttttc aaaggctatc
     1441 tctttgaggt ttttcccacc tgcatctgcg ccataaatcc agtagcctgc ttttttaagc
     1501 tcttctactg cacgaacaag gtttggcacc acagccatgg ctacccaagc acttgcaccg
     1561 gagcttgtcc tatcgattat ctcgctttca agggcactct cacgttttgg ggcgatgaca
     1621 aggtctaccc caaactggtc tgcagagcgt aaaattgcgc caacattttg cgggtcgctt
     1681 atagaatcaa gaacgacaac aatcgattct tccttctctt ctttagacac agaaagtgta
     1741 gaaaggaatg cagttaaatc gactctttct tttttttcgt ctttttcacc ttctataatc
     1801 aatacaaggc cgcgatgttc ccttgcaaag tctgaaagtc cagaaacaag ctggtttaat
     1861 tctgccttgg aaacttgctt gctctgaata ttcagttttg tagcctcaga aagtattttt
     1921 tttatccgtg gtccccttgt atcaaaatac aaggtataac gaattgcggt acttttttca
     1981 agatttttac ttttataaaa acgcaagcgc tcttctatgg catgaaaacc cgttattgtt
     2041 tttttcatat atcatccttg gtgttttttt ctagattttt acttttataa aaacgcaagc
     2101 gctcttctat agcatgaaag cctgttattg tttttttcat atatcatcct tgtttttttt
     2161 tattatcagt taatacgagg cttattttat caaaatcttt ttttatttgc taggagaagg
     2221 aaatcagact aggggcaggt tctctagaaa atgccgcaga attgcgagta aaatggcttg
     2281 cgataaggct taaaatagcg taccatgttt ttatggcaaa aaatcgtata caagaagctt
     2341 tggcaaaaac tgacttaaaa gaagcgcttc tcaagcgttt tatttcatat gtacaaatcg
     2401 atacacaaag caatgccgca gaggctgatg caggtagtat cccttcgact gagggacaaa
     2461 aggagcttgc aaggctttta gaggcagagc tcaaagaact tggctttgac gagctctttg
     2521 tctcggaaca tttttatgtt tgtgcgcgct taaacgcaac aaaggctaag gaaaaaacgc
     2581 caagtctttg ttttttagcc catatggata cctccgatgc ggtgagtgga aaagatgtac
     2641 aagttaaaat aagcgaaaac tacgatggta gagctattgt gcttggttct ggtccagaag
     2701 ggcttgctct cgatccagaa cttgaaccag aacttctcga ctgccttggt gacagtatca
     2761 tccatactga tgggaaaacc ctccttggcg ctgacgataa ggctggcata gcagaaatta
     2821 taacagcact tgcatacatt atagaagaaa acattcctca tgggcaaatt gaacttattt
     2881 tttctcccga tgaagagacg ggacatggca tggataaggt gccatttgac tggatagaat
     2941 ctgagttttg ctacaccctt gacggaagcc atcttggtga ggttgagtac gagtgtttca
     3001 atgcctacaa aagcgatatt cgttttatcg gtaaggcagc gcatacaggc accgctcgtg
     3061 gcaagcttat aaatgccctt acaatggcag ctttttatat tggactttta cctcgtaatg
     3121 aaagccccga aacaactgat ggctacgaag gctttttcac cgctatggac ttatcaggaa
     3181 atatcgaaga agcaaatctc actgtcttta ttcgcgactt tgaaacagag tccatgcaaa
     3241 atcggctcaa acggctggaa atttttgcag aagcgacgga agcgcatttt cccggaagca
     3301 aggtagaaat acagcatacc gagcagtata aaaacatgaa agatgcactt ctcaaaaagc
     3361 ccgaagccat gcagcttgta aaaaaagccc tcgatgagct tgatatagaa atgatttcaa
     3421 aaccgattcg cggtggcaca gatggatcac gcctcacaga gatgggaaaa cccactccaa
     3481 atatttttac cggtgggcat tcttttcatt ctcgaactga atgggcatcc ctttcacaaa
     3541 tgatttgggc aacggaaaca attatacgca tcataaaaaa tgcataaaga acgataaaaa
     3601 agcgaggaga aaaggaaaaa atatgtacga atactgtata gaaggtggct ttcccataaa
     3661 gggtacaatc aaggcaagtg gcaataaaaa tgctgccctc ccctgcattg cagcaagttt
     3721 gctctcttct gagcctgtta tcttgcacaa tatccccgaa atcgaggatg cgggtgttat
     3781 gctcgatgtg ctaaaagaac tcggtgcttc cgtagaaaat ctcggaaaca atagctggaa
     3841 aattcatgca aaagatattc acacgcatac tattcccctc gagtcggcaa aaaaaatacg
     3901 tgcctccatt ctctttgcag gccctcttct tgcacgactt ggcaaggtag cgcttcctcc
     3961 tcctggggga gatgtcatag ggcgacggag gctcgacacg cactttttag cgcttaccga
     4021 acttggtgcc cgtgtcgaga tggataggca gtttaccttc tcagcgaaca agcttgtcgg
     4081 ggaggatata tttctcgatg aggcttcggt aactgctacc gagaatgcca ttatggcggc
     4141 aagtcttgca acaggcaaga cgattctctc aaatgccgca agcgagcccc acgtccaaga
     4201 cctttgcaaa atgctcaatg ccatgggggc caaaatttct ggcattggtt caaatattct
     4261 cacgatagat ggggtagaaa gtctcggagg atgcgaatac aaaataggac cagactttat
     4321 ggaaatcggc tcctttatag ggcttgctgc tgcaacaaag ggaagtatca ccatcaccga
     4381 cattgaacca cgagatttac gtcccataaa acttgccttt gcaaaacttg gcattcactg
     4441 ggaaattaac ggaacaaact tactcgtacc aactggacaa gaaatgcggg taaaccctga
     4501 cctcggcggc atgattccaa aaattgacga tgcaccctgg ccaggttttc cgcctgattt
     4561 aacgagcatt atgaccgtcg ttgcaacgca ggttgaaggg acagtgctta ttcacgaaaa
     4621 aatgtttgaa tctcgtatgt tctttgtaga caagctcatc gccatgggag cacgcattac
     4681 gctctgtgac ccgcaccgag ctgttgtctc aggaccaagt accttgcatg gaagcgagct
     4741 tatatccccc gatgtgcggg ctggtatggc gatgattatt gcagcaatga gtgctcgtgg
     4801 agaaagcgtc ataaaaaacg tctatcaagt agaacgtggc tatgaaaacc tcaccgaacg
     4861 attaaaatca ctcggtgcga ggattgaaag acgaagttct tgctagattc tcttttgctt
     4921 ctggttccgc gtctatttgc ttagatgatt tttatcaata aggaagaaac ttaatctgag
     4981 cgtagagcgg aaaagataat tgcactcagc agcaccaaaa cacagaggat taaaagatat
     5041 acccaaggtg ccactccccc aaatatacca atgattacaa aagaaaaaag gtttaaaaga
     5101 tacaagattg tttccaaaac aaaatacagt ataaacgtaa acaagggaag ggttataagc
     5161 caaacgaact taaaatgcgt ctctgccccc aatttataat tccaactaat ccctgcaagc
     5221 aaaatgagca caattaataa aacaaaggga taactcattc tatccataaa tgtttgaaaa
     5281 taaacttcct tagaaaaacc gtagctatct gccttttttg caaaggaagc taaaacagtc
     5341 aaaggcataa ccttcacccc taaggacgct ttacagattt gcaaaaaatc atcaaagggc
     5401 attgccaaga ggagtgaatt ataatgattt gagccaactt ctctttcata gctaaaactc
     5461 ggaccaagat agagaccatc cttttcagag tcggtggata caagataaat aaggggaaca
     5521 aattgtgaag attctttttt tattttaaat cttttataca tatctgtctg aaaagtctgg
     5581 ggtgtttgtt ctagaggaaa ggcaacaagt ttcgcataag gtacagaaaa agaaaactcg
     5641 agttctttgt tttcatcgta agaaaaaacg tttaactccc tcagatattg tattaaattt
     5701 cctgcttttt cttttgctgt ccctcccttc cagtagagaa ggaaaaagcc accctctggt
     5761 ttttttacag aaaaatacac atttttatat tgttcaaaat tgataagatc ttccacttca
     5821 tttgaaaaaa aatactttcc atccatcttt tctttggaaa gaagaaagta tcgatcgata
     5881 tctaagtcag ccggatacgc tgttttcaaa tccgaaaaaa tataataggc tttttcaaac
     5941 tcttggttta taaaagccac ataacccttt ctctttgttt cataggcaag cacctgctca
     6001 ccatctagcc ccgtgggatt atcttgcaac ttgttccagg catctgccgc caactgctgc
     6061 gcctcttgac ggtttccatc ttttttctct gtcatctcta gcaacaaatt cgcataataa
     6121 tgcgcattga aaaaatcttc cttcaaaaaa gcttctcgtg ccctttccat caactggggg
     6181 ctcgtgtagc cataatcttg tacgctaatc ggagagctta cttgttcttg catctcacga
     6241 tctcgaatct tttgcttcat tccagaataa aatatatcga ttgtatcttt taagtcgagc
     6301 gcttccacag catttggttt aagagccaag gcattatcga tataatactt cgcataaata
     6361 taatcatccg cgtaggcaga ttcagccgcc aagagaagat aggcttcaaa atttttttcc
     6421 ttattttcca tgttttcttg tgtattttgc aacagtggaa taaaaacttc tgaagaaagc
     6481 atacaaagca ctatacaagt aaaactcaag gcaataacgt ttttaaaaaa agcaactcgt
     6541 atcgttgaaa aacgctcaac tttttcggaa tcagaacgac caaaggacca tgaaagcccc
     6601 acaagaaaag cgctcaaaag aaggctcggt aaaaacttaa gaaatacaag taaggcttca
     6661 tagattttgt attgtgtcgc gtaggaagca aattcctcag gaacaggact cgtaaaaaaa
     6721 acaaagagtc caatgccaac aaaggaaaat aaaagataga ttaaaataac tgctataacg
     6781 tgcttatata ttttcatccg atactcactt tattttttgt ctttgacgta taaaatgcac
     6841 caaaccaatc aaacaaaaac aaagtgtaat acttatgttt atcacaaatt gtatattatt
     6901 tcggtttgta aaccaaagca tattattgtg ttgaaaaaaa ccaatcattt tatcaaaaag
     6961 cactgctgaa aaaacttctc tatttatttt acaaatacta acaaaaagcg caacaacaag
     7021 agaaacattg ataagtttcc atgaatttat atttctaaca aaaaagatgc tcaatagagc
     7081 caagccaaaa ctcgcaaaca tgagatagct aagatatcct cgagcaagac tttttattgc
     7141 ctccgtgcgt gcaaaataaa aaaaggtttt tagaacctct aaaacaggcg gtaaatctat
     7201 tgttttttca aacaatggat cggcataaaa ggcttgttct ggcacttgca catcctcatc
     7261 aaaaaaaaca gactcgatat gccctgagct ggaagtatcg atgcgtacac cttctgctcc
     7321 tccttctggg aaaaacctag caatataatc aactgaggac tttgtttgac ggaaaaatcc
     7381 ttcagaaagg cgtcttgctt catattcaga aacaagaggt ttttgtgcaa gctgcattga
     7441 aagcatccat ggaatagcaa aaagccagat actaaaacca atgatgaaat agaacaaggt
     7501 agaaagaata gatatattcg gatgccggat aaaataaaaa ataagaccga ggcaagcaaa
     7561 aacaagacaa aagggcaggg ccttaatcaa aacctgaaaa aacaatgcaa aattgacaaa
     7621 ggaaaggttt tcacctgcta ccattgaaaa acttaaatgt ataagagaaa tcgccaccgt
     7681 agaaacaaag gctgtagcta ttgtaaggat gataaaaaca aataccataa aaaaggcgtt
     7741 tttcatagat atagaatagc atgaagagaa aactaataca agattttttt tctagcaact
     7801 tatc
//
LOCUS       DAOSSW010000080         7707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_8922_length_7707_cov_6.742290, whole genome shotgun sequence.
ACCESSION   DAOSSW010000080 DAOSSW010000000
VERSION     DAOSSW010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8922_length_7707_cov_6.742290"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..801)
                     /locus_tag="PKW26_06920"
     CDS             complement(121..801)
                     /locus_tag="PKW26_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93333.1"
                     /translation="MQKQDLIDRSPVRFLEKATNGGLQEGEVAIITSKKGLGKTSVLV
                     QIGLDQLFQDKNVVHVSFNQQSDFVMRWYEDIFTEMAKKKNLQLAKDIKSDIVKNRVV
                     LNFNQDTFNISSIVSTLKALEVGGVKTDALIIDGLSKTKLNAEAMDQFKAYAKEAKAS
                     IWFSQNTEGETLETVLPKEITSKADAIVHLAQKPDTIQMEVLKVRGETIKDSNLRLDS
                     KTLLMFEK"
     gene            complement(811..2901)
                     /gene="fusA"
                     /locus_tag="PKW26_06925"
     CDS             complement(811..2901)
                     /gene="fusA"
                     /locus_tag="PKW26_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOQ93334.1"
                     /translation="MSFDISKMRNIGISAHIDSGKTTLSERILFYCNKIHTIHEVKGK
                     DGVGAVMDNMELEKERGITIQSASTQVNWKDHTFNVIDTPGHVDFTVEVERSLRVLDG
                     AILVLCSVGGVQSQSITVDRQLKRYHVPRIAFVNKCDRTGANPFKVRMQLREKLGLNA
                     YMVEIPIGLEDKLEGVVDLITMKALYFEGESGTELRVAEIPAHLVEEAEKYREELLDA
                     ASMFSDELMEAMLEDTVTEDMINDAIRIGTLSEQFVPVYLGSAHKNKGIQPLLDGVVK
                     FLPNPTEVKNYALDLDNNEEEVLLSCDEKSPTVSLGFKLEDGQYGQLTYVRIFQGSIK
                     KGDELFNTRARKKFKVGRLVRMSSNTMEDINEGVPGDIVALFGVDCASGDTFCSPSVN
                     YAMTSMFVPDPVISLAITPKDKKSSDQMAKALNRFTKEDPTFQTYVDPESNQTIIKGM
                     GELHLEVYVERMKREYKAEVETGMPQVAYRESITQQAEFNYTHKKQTGGSGQYGRVAG
                     FMEPLADKDYEFVDAIKGGVIPNEYIPSCDKGFKASMHKGSLIGYPIVGVKCTINDGQ
                     YHPVDSSDIAFQQAAIGAFREAYLKAKPVILEPIMKVSIEGPTEFQGNIFAVINQRRG
                     IIVSSTEDGTFSRVESEVPLSEMFGFSTILRSLTQGKAEFTMEFQKYGKVPTNISEAL
                     VKEYEEKRKKEQGK"
     gene            3117..3908
                     /locus_tag="PKW26_06930"
     CDS             3117..3908
                     /locus_tag="PKW26_06930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOQ93335.1"
                     /translation="MNTSEYTLITGASAGVGKEMAYMLAKKQKNLVLVARSERVLEAM
                     QKELQDSYGISVLVFAIDLSKEGAAQRLFSFCQEKMFVSLLINNAGVGAFGESVALGN
                     SVLPMLYLNILSLTELCAFFGKEMKERGRGSILNIGSIAGNQPTSFFASYAASKSYVL
                     NYSLALRHELRPFGVNVTCAQPGYIRTGFDDTCNIKSEKYKRFSYKNGMSAKNVAKKA
                     LAAVFRKRSFVRIGFTNKIVAFVSTFIPRNLLAFLMSKSIRSMTK"
     gene            3934..5127
                     /locus_tag="PKW26_06935"
     CDS             3934..5127
                     /locus_tag="PKW26_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ93336.1"
                     /translation="MKSNIAVLPKLFAKSPRLAFKMFRAFFRLELAKRSKKQSYGYDH
                     MKNFCLIYMKLTPLCNLHCVMCGQRGDKGVLKGSVGAAEAKKILSFEQYKKMVDELAP
                     KKPIYYLWGGEPFLYPDLFPLVAHIQKTGGFCSVNTNGTMLEKYAEEIVRNKWHALFV
                     SLDAFEDVNDSIRGKGSYEKVIRGFEAINREKKKQGSNLPYMGVVTTVTNKNYESLAD
                     LAEASKDFDIDWHIYNLGTYTNDNIVALQKKLMKEKFDMDIDCLEGYNTGYNKNIDAY
                     VLHDVLEKIHATNYGHPSITVPVLNPEKTQVYYDELETPVRNHCVVPWCQTNINYNGD
                     VHFCADYPDYILGNITEQSFKEIYNGDRANTFRKEMMESEGGIFPGCLRCYQNMLFGK
                     KIKGF"
     gene            5128..5598
                     /locus_tag="PKW26_06940"
     CDS             5128..5598
                     /locus_tag="PKW26_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93337.1"
                     /translation="MYKPQVAPVPFINMPPPRLNYPLMKLIRLIIKPYMRSLTFYDPE
                     ILFPERFVKAYKKCTDKKIRLILAFRHAYGDDPQLMAALMHYSLPRYAKKIGIALPKF
                     THAHFIYGAEVPMWSDASVLLQEYTKLQSLILEKQFEESIFEYRSIYPKNPKKA"
     gene            5674..6990
                     /locus_tag="PKW26_06945"
     CDS             5674..6990
                     /locus_tag="PKW26_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOQ93338.1"
                     /translation="MILSPELECMNRKDLRVLQFARLKNLIEKIYANVPFYKEKFDAL
                     GLKPSDIRCLEDIKKLPFTTKDDLRQTYPYGLLAVPQSEIVEIHMSSGTTGLPVVNAY
                     TQADMDQWTEGMGRTLSGAGATRNDTVQNAYGYGLFTGGMGVHYGTQRIGATVIPISS
                     GNTEKQLMLMRDFHSTLFTCTPSYALYMAEKAESMGINVHDLPVRAGCFGAEPWSENM
                     RKEIEKAWNIKAYDIYGLTEITGPGVAFECEAQDGMHFNEDLWYPEIIDPETGEPLPD
                     GEKGELVITTILKEGTPLIRYRTRDITYIMKEACSCGRTTRKIHRLFGRTDDLLILRG
                     VNVFPSQIEHALIEIQGVQPNYLIVVDRSEKTHLDDVELHVEVSIDQFSDETRGMEAL
                     RSKIEAVMKSKLGISVKVKLVEPMSIERSIGKAKRVIDKRNLYNKD"
     gene            7021..7437
                     /locus_tag="PKW26_06950"
     CDS             7021..7437
                     /locus_tag="PKW26_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOQ93339.1"
                     /translation="MGIQQISIFLENTAGRIADVTGVLKDAGINLRAIMIADTADFGI
                     LRVIADDSEKALAVLKEHKFTTRTTDVLAVHISDAVGSLHEVLTIFDKHEINIEYIYA
                     SLEKTGQSAVIIFKIEDCKKAKEVLCDIGFDCIDSF"
     gene            complement(7523..>7707)
                     /locus_tag="PKW26_06955"
     CDS             complement(7523..>7707)
                     /locus_tag="PKW26_06955"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOQ93340.1"
                     /translation="SLHDNVGMTMSKIRHTMCNCGALTIEELQKKAKLTLVSQASIAE
                     GASHDVIVKNTSIRAE"
BASE COUNT         2293 a         1419 c         1571 g         2424 t
ORIGIN      
        1 gaggtttgca gcttagaaat ctcttaggaa aaattctagg caaacttttg tttgaaaagg
       61 taatgctagt tgtagagcaa ggtgcaacga tgtctcgcac cttgctaata ctttgtgcgt
      121 ttatttttca aacatcaata atgtttttga atcaagtcga agattggagt ctttgattgt
      181 ttctcctcgc acttttaaga cttccatttg aatggtgtct ggtttttgtg caagatgcac
      241 tattgcatct gcttttgaag tgatttcttt aggcaaaact gtttcaagag tttcgccttc
      301 tgtgttttgg ctaaaccaaa tacttgcttt tgcttctttt gcataggctt tgaattgatc
      361 catagcttct gcattgagtt ttgttttgct caaaccatca ataatgagtg catctgtttt
      421 tactccacca acttcaaggg ctttgagtgt tgaaacaata ctactaatat taaaagtatc
      481 ttgattaaaa tttaatacaa ctcggttttt tacaatatca gatttaatat cttttgcaag
      541 ttgaaggttt ttcttttttg ccatttcagt aaaaatgtct tcgtaccatc tcataacaaa
      601 gtcagattgt tgattaaaag atacgtgtac aacatttttg tcttgaaaca attgatcaag
      661 cccaatttgt acgaggaccg aagttttccc caagcctttt tttgaagtaa tgatagcaac
      721 ttcaccttct tgtaagccgc catttgttgc tttttctaaa aaccgtactg gacttcggtc
      781 tataagatct tgtttttgca tgaattcccc ctatttacct tgttcttttt tgcgtttttc
      841 ttcgtactct tttacgagag cttcagaaat attcgttgga acttttccgt atttttggaa
      901 ttccattgta aactcagcct taccttgcgt taaagaacgt aatatggttg agaaaccaaa
      961 catttcgctg agcggaactt cagattcaac acgcgagaag gttccgtctt cggtagacga
     1021 aactattata ccacgtcttt ggttaattac agcaaaaata tttccttgaa attctgttgg
     1081 tccctcgatg gaaaccttca taattggctc aaggataaca ggttttgctt ttaagtaggc
     1141 ttcgcggaag gcgccgattg cagcttgttg gaatgcaatg tccgaggagt caacagggtg
     1201 gtattgtccg tcgtttatcg tacattttac acctacaatt ggatagccaa tgagagaacc
     1261 cttgtgcatt gaggctttga atcccttgtc gcaagaagga atgtattcgt ttggaataac
     1321 accgccctta attgcatcga caaattcgta atctttgtca gcaagtggtt ccataaagcc
     1381 ggcaacacgg ccgtattgac cagaaccacc agtttgtttt ttatgggtgt agttgaattc
     1441 agcttgttgt gtgattgatt ctcgatatgc aacctgtggc atacctgttt caacttctgc
     1501 cttgtattca cgtttcattc gttctacata gacttcgagg tgaagctctc ccattccctt
     1561 gataatcgtt tgatttgatt cagggtccac gtaggtttgg aaggttgggt cttctttggt
     1621 aaaacgattg agcgcctttg ccatttggtc tgaagatttt ttatcttttg gtgtaatcgc
     1681 aagagagata acagggtcag gaacgaacat acttgtcatt gcgtagttta cagaaggtga
     1741 acagaaggta tctcctgaag cacaatctac gccaaagagg gctacgatgt cgccaggaac
     1801 accttcgttg atgtcttcca tggtgtttga actcatgcga acgagtcggc caactttgaa
     1861 tttttttcgt gctcttgtgt tgaaaagctc atcgcctttt ttgattgaac cttggaaaat
     1921 acgtacatag gttaattgtc cgtactgacc gtcttcgagt ttaaatccta aagaaacagt
     1981 tggagatttt tcatcacagc tgaggagtac ttcttcttcg ttattgtcga ggtcgagggc
     2041 gtagttttta acttctgttg ggtttggaag gaattttaca acaccatcta aaagcggttg
     2101 aattcccttg tttttatgtg ctgatccaag atagacaggt acaaattgct cagaaagcgt
     2161 accaattcgg atagcatcgt ttatcatgtc ttcggttacg gtatcttcga gcattgcttc
     2221 catgagttca tctgagaaca ttgaagctgc atctaagagc tcttctcggt atttttcagc
     2281 ttcttcgacg agatgtgcgg gaatttcagc aacgcgaagt tctgtaccgc tttcgccttc
     2341 gaagtaaagc gctttcattg ttataaggtc tacaacacct tcgagcttgt cttcaagtcc
     2401 gattgggatt tctaccatgt aggcattgag accaagtttt tctcgaagtt gcatgcgcac
     2461 tttgaagggg tttgctcctg ttctatcgca tttgtttaca aaggcaatgc gaggaacatg
     2521 ataacgcttg agctggcggt caacggtaat tgactgagat tgcacaccac caacagaaca
     2581 aagcacgaga attgcaccgt caagtacacg tagggatcgc tcaacttcta cagtgaagtc
     2641 aacgtgtcct ggggtatcaa taacgttaaa tgtatggtcc ttccaattta cttgtgttga
     2701 tgcggattga atcgtaatgc cacgctcttt ttcgagctcc atgttatcca taacagcgcc
     2761 aacgccatct tttcctttta cttcatgaat tgtatgaatt ttattacaat agaaaagaat
     2821 ccgctctgat aaagttgttt tcccagaatc aatgtgagcg ctaataccga tattcctcat
     2881 tttagaaata tcaaaactca taggttctac ctctcaaata taatttttac agtatagttt
     2941 ggtactatct gcaaggtttt caaaataaaa acatcatatg cattttactt attttttttg
     3001 atagtatcaa gggggaagct aaaaaaaagg acatttatga agaaaaacag ctatgttaaa
     3061 tagacaaaat agcatatact gtgaggatat tgcttttcgt attaaggaaa aaagaaatga
     3121 atacatctga atatacgctt ataactggtg cttcggctgg tgttggtaaa gagatggcat
     3181 atatgcttgc caaaaaacaa aaaaatctag tacttgttgc ccgctcggaa agagtgctag
     3241 aagcgatgca aaaagaactg caagatagct atggtatttc tgtccttgtt tttgcaatag
     3301 atctttcaaa agagggagct gcacaaaggc tctttagttt ttgtcaagaa aaaatgtttg
     3361 tttcgctgct tataaataat gcaggtgttg gcgcctttgg ggaaagtgtt gcactcggca
     3421 actcggtact ccccatgctg tatttaaata tactcagcct tacagaactt tgcgcatttt
     3481 ttggcaaaga aatgaaggag aggggcagag gttccatctt aaatattggg tcgattgcag
     3541 gaaatcagcc aacttcgttt tttgcctctt atgccgcaag caaaagttat gttttaaatt
     3601 attcgcttgc acttcgccat gaacttcgac catttggggt aaatgttact tgcgcacaac
     3661 ctggatatat tcgcacgggc tttgatgata catgtaatat aaaaagcgaa aaatacaagc
     3721 gtttttcgta taaaaatgga atgtccgcaa aaaacgttgc aaaaaaagcc ttggcagctg
     3781 tctttagaaa acgttctttt gtacgcattg gtttcacaaa taaaatcgta gcttttgtat
     3841 ctacgtttat tccgcgcaac cttttagcct ttctcatgtc caagtccata cggtctatga
     3901 caaaataaaa acatgcacaa ataggaaaat cttatgaaat caaatattgc tgtattgcca
     3961 aaattatttg cgaagagtcc acgccttgct tttaaaatgt ttcgcgcttt tttcaggctt
     4021 gaactagcaa aacgaagtaa aaaacagagt tatggttatg accatatgaa aaacttttgt
     4081 cttatttata tgaaattgac accgctttgc aatttgcatt gtgtcatgtg tggacagcga
     4141 ggcgacaagg gagttctaaa aggttctgtt ggtgcagctg aagcaaaaaa aatactcagc
     4201 tttgaacaat ataaaaaaat ggttgacgag cttgcaccta aaaagcccat ttattatctc
     4261 tggggaggag agcccttttt gtatcccgac cttttccctc ttgttgcaca tattcaaaag
     4321 acaggtggct tttgtagtgt aaatacaaat ggaaccatgc ttgaaaaata cgctgaggaa
     4381 attgtacgaa ataaatggca tgcgctcttt gtctccctcg atgcctttga agatgtcaat
     4441 gattctattc gcggaaaggg ctcctatgaa aaagttatac gaggttttga agctatcaat
     4501 agagaaaaga aaaaacaggg ctcaaatctg ccatatatgg gggttgtaac aaccgttacc
     4561 aataaaaact acgaaagttt agcagacctt gcagaagctt ctaaggattt tgatattgac
     4621 tggcatattt ataatctcgg tacatataca aatgacaata tagtcgcact tcaaaaaaag
     4681 cttatgaagg aaaagtttga tatggatata gattgtttag aaggctataa taccggctac
     4741 aataaaaata ttgacgccta tgttttacat gatgttttgg aaaaaatcca tgcgacaaat
     4801 tatgggcatc cttccataac ggtgcccgtt ctcaatcctg aaaagacaca ggtctattat
     4861 gatgagctag agacgcctgt tcgaaatcat tgcgtcgtgc cttggtgcca aacaaatatc
     4921 aattataatg gggatgtgca tttttgtgct gactaccctg attatatttt gggaaatatt
     4981 accgagcaaa gttttaaaga gatttataac ggtgatcgtg caaatacctt tcgcaaagaa
     5041 atgatggaaa gtgaaggcgg aatttttcct ggttgcctca ggtgttatca aaatatgctt
     5101 tttgggaaaa aaataaaagg attttaagtg tataaaccac aggtagctcc cgtacctttt
     5161 ataaacatgc ctcctcctcg tttaaattat ccactgatga aactcattcg tttgataatt
     5221 aaaccctata tgcgctcctt aactttttat gatccagaaa tcctgtttcc cgagcgtttt
     5281 gtaaaagcct ataagaaatg tactgataaa aaaattcgac ttattcttgc ctttcgccat
     5341 gcctatggag atgacccaca actcatggca gcattaatgc attatagttt accacgctac
     5401 gctaaaaaaa taggcattgc tctgccaaaa tttactcacg ctcattttat ttatggggca
     5461 gaagtaccca tgtggtcaga tgcctcggtt ttattgcaag aatacacaaa actacagtct
     5521 cttatcttgg aaaaacaatt tgaagaaagc attttcgagt atagaagtat ctatcctaaa
     5581 aatcctaaaa aagcttaagt tttgagtaca gatagcataa atataaatgt tttgcttgtg
     5641 aaaaaataac tagaatgcta tacttatttt attatgatat tgagtcctga attagaatgt
     5701 atgaaccgca aagatttgcg tgttttacag tttgcacgtt taaaaaatct catagaaaaa
     5761 atctatgcga atgtgccatt ttacaaagaa aaatttgatg cactcggttt aaaaccaagt
     5821 gatatacgtt gtttggaaga tataaaaaaa cttcctttta ctacgaaaga cgatctacgg
     5881 caaacttatc cctatggttt gctcgctgtt ccacaatcag aaattgtaga aattcacatg
     5941 tcatctggaa cgacgggctt gcctgttgta aatgcctata cacaagccga tatggaccag
     6001 tggacggagg gcatgggacg aaccttgagt ggtgctggtg caacgcgaaa cgataccgta
     6061 caaaatgcct atggctacgg attatttacc ggtggaatgg gtgttcacta cggtacgcag
     6121 cgaataggtg caaccgttat tcctatctct tctgggaata cagaaaaaca actcatgctt
     6181 atgagagatt ttcattcaac tctttttacc tgtaccccct cttatgcctt gtacatggca
     6241 gaaaaggcag aaagcatggg gataaatgtg catgacttac cggtgcgcgc tggatgcttt
     6301 ggtgcagagc cttggagcga aaacatgcgc aaggaaatcg aaaaagcatg gaatattaaa
     6361 gcctatgata tttatgggct cacggagata acaggacccg gggttgcctt tgaatgtgaa
     6421 gcacaagatg gaatgcactt taacgaagat ttatggtatc cagaaattat agatccagaa
     6481 acaggagagc cgcttcccga tggcgaaaaa ggcgagcttg ttataacaac gattttgaaa
     6541 gaagggacgc cccttattcg ctatcggact cgtgatataa cctatataat gaaagaagct
     6601 tgttcttgtg ggcgcacaac tcgaaaaatt caccgacttt ttgggagaac agatgacttg
     6661 cttattttac ggggcgtcaa cgttttccca agccaaattg agcatgcact tattgaaatc
     6721 caaggtgtgc agccaaacta tctcattgtg gtggatcgca gtgaaaaaac acatctcgac
     6781 gatgtagagc tacatgtcga agtgtctatc gaccaattct ccgatgaaac tcgtgggatg
     6841 gaagccctgc gctcgaagat agaagcggtt atgaaaagca aactcggcat aagcgtgaaa
     6901 gtaaaactgg tagaacctat gagtatcgag cggtcgatag gtaaggcaaa acgggtaatt
     6961 gataagcgaa atctatacaa taaagattaa gctttttcat taactttttt aggaggaatt
     7021 atgggaattc aacaaatttc aatttttttg gaaaataccg ctggtagaat agctgatgta
     7081 actggcgttt taaaagatgc tggtataaat ttacgtgcaa tcatgattgc tgacaccgca
     7141 gactttggta tcttgcgtgt tattgccgat gattcagaaa aagcacttgc tgttctcaag
     7201 gaacataaat ttaccacacg aacaaccgat gtcttggcgg tacatatttc cgatgccgtt
     7261 ggttcacttc acgaagtctt gacgattttt gacaaacatg aaatcaatat agaatatatt
     7321 tatgcatcgc ttgaaaaaac aggacagtcc gctgttataa tatttaaaat agaagattgc
     7381 aaaaaagcga aagaagtact ctgcgacatt ggttttgatt gcatagatag tttttagtct
     7441 gtgttgatga agatttcatt agatttagag ataaaaaaaa gcttcgtact ttagcgaagc
     7501 ttttttttat gagctttttg ctttattcag ctcgtatgct tgtgtttttt acaataacat
     7561 cgtgagatgc accttctgca attgatgctt gggaaacaag ggtgagtttt gctttttttt
     7621 gcaattcttc tatcgttaaa gcaccgcaat tacacatagt atggcgaatt ttactcattg
     7681 tcataccgac attgtcgtgc aagctgc
//
LOCUS       DAOSSW010000081         7535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_9155_length_7535_cov_10.394385, whole genome shotgun sequence.
ACCESSION   DAOSSW010000081 DAOSSW010000000
VERSION     DAOSSW010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9155_length_7535_cov_10.394385"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PKW26_06960"
     CDS             <1..608
                     /locus_tag="PKW26_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOQ93341.1"
                     /translation="ADAVICAGDFAEVFKEETGLPVLEKLIKKHETIFSVLGNCDPPS
                     FLEKLESYDVSVQGLIASFEGLTFIGAGGATRFTGQTPNEISEEEILGDIALVQNGED
                     APWDNLVMIIHNPPHDTKLDKVSMGLHVGSKKIREAVEEIKPLVLVSGHIHESFAIDS
                     LGDTLLINPGSLAEGRYAILEIEKKNGVFEARAELKEIIVP"
     gene            complement(614..1654)
                     /locus_tag="PKW26_06965"
     CDS             complement(614..1654)
                     /locus_tag="PKW26_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOQ93342.1"
                     /translation="MKRKHLYSIFFICIAAALFSQSAVNEWRWLHKTEEKQSLSQLPE
                     HLKMYSFASKSEIEGLESPFVLGESVQSSVVKLILSPFEINAYETTYALWYEVYQQAL
                     ERGYLFQNPGQEGSEGKRGKEPTEEGRFEPVTTISWRDALVWCNALSELRGYTPCYTF
                     DGEILRNSADSAKIDLVVCDWTADGYRLPTEAEWEWAARKTKTGFQKGNLPSGSVNAK
                     GQSDDSVLETDIAWTSGNTTKTEKVGTAGTLFSKNAEVGSGRANGAGLFDMSGNVLEY
                     CWDWFADYVQPFDIRDRYTGPDFGYERVSRGGSWSPYASFIYAGDRYAFDPDIAFNYM
                     GFRLCRSLKPLY"
     gene            1876..3879
                     /locus_tag="PKW26_06970"
     CDS             1876..3879
                     /locus_tag="PKW26_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93343.1"
                     /translation="MFISMLSSKSYEFIDQNIDEVLFVLSLEEGKTIIGDDTVGGKAT
                     FRAVGENFSSVFDLFLAQQRFYVEKKDDLWLVSKMRVLCDDENVLQVDAFDIQPQRLF
                     EQISSVSGVPILFDIIPTQEISVHVSNLSVKDCLVLILASLGNYTVTEEKNMLRIQRE
                     ERQMIENKNYGYCRIEKSAKRENAEPSAENRFIEGDYFSVEISQAIGKNVLEELCERA
                     EKEVVFLAPCDALIETIQLKDKSFQELLDLFCMQLYVSAIEKDGVLYFVEGQDTLFNS
                     KNFGKTWDMYKLDYLKSQEFSSFLQNRFENLRMYTISSSLLFVLANDKIHKEIQSLIQ
                     IVDIQKQPFIVSLQFIKAEEFFAHLPPGYTADEFYRMGHDGELFYLGTEKKYESLLGL
                     VEKIDLPAKQILYDLLVLQVQDSSSFSWEPSLSAKTISAVERPFVSVDLGSVLDLQFN
                     VLSAFGINFAVNLQTAINESKAKIFADTSLHGLSGTPIHFQNTNTYRYKDIAINPETG
                     KPMYTGITREIVSGLFLEVEGWVSGSGMITTKVKATISKRGADVSLSAGNPPPTYEKI
                     ITTEVRSKSGETVILSGLIQDDSVLIEQRIPLVSKIPIIGKLFSSLINTREKTEMLIY
                     LIPKLVNDDEENKLEIRDKYQERMDDITKNIFHFLKEECGYEF"
     gene            3869..5065
                     /locus_tag="PKW26_06975"
     CDS             3869..5065
                     /locus_tag="PKW26_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOQ93344.1"
                     /translation="MSFNLSSKFCAYNKVVQLENSEMQCVIGLSQENDEIKNIIAKNF
                     NQKRRSERQRLLFRKISTEECLRKLSKEFSNALFLKKVEEKEKEKSHFDDVPIKNILN
                     SMIIECLENKGSDIHLQRWDNFYELRFRINGTMKTQSKINAEIGDALMQRVKVLSKLD
                     LAEKRKCQDGRFDFLKGDYEAELRVSVIPCFYGESLVLRILDRKKNIENFSSLGFTGE
                     QEKMLDEIVKSTSGLVLICGPTGSGKTTSMSSILGEIQKQNKKIISLEDPVEYIIPNV
                     TQVQINKNLGLDFPTLLRSVIRQDPDIVAIGEIRDSATAKIAVQTALTGHLVLATLHT
                     DSARDSFFRLTDMGVPAYLLSSVLKWVICQELVVLPNSRKLNASITYCDSDFLSELKE
                     ECRYGS"
     gene            5055..5987
                     /locus_tag="PKW26_06980"
     CDS             5055..5987
                     /locus_tag="PKW26_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOQ93345.1"
                     /translation="MAANLFYIFCSNLLGFLQGKFSLQESLEIMISHEATGKKIKNFT
                     SYILSELEKGSPFTTILIANPYILIKKQYQLLFYSEEKTGDYKKSLDYVCKSEKEKNE
                     TIEEVIKLSLYPLFIILLCIFGSIAFIFYSKDFPVYVQQASVFSGIAYANIFLVFFLL
                     LFAFSFYMLHKKPIKNIFLICLDFFLAQSFDFTTALEYASYVFDDKKNIRQIVLDSLS
                     GLSLGLELSTVFDKTGLFTKSEIKTLDLYIFNGNLRGGIDIVLKELERKAQKNKDIFI
                     SLVEPSLIICAGIYFLLIVIHSLLPYLTFYGGLL"
     gene            5984..6424
                     /locus_tag="PKW26_06985"
     CDS             5984..6424
                     /locus_tag="PKW26_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOQ93346.1"
                     /translation="MKKKHDEAFTFAETIASLAIILILSCSVGFAAFKFIDRAKEVSV
                     QTQILQFKTALNAYYLDCGFFPNEAQGLEALWEKPFLEPVSSEWKGPYTDKKIPKDPW
                     GNFYVYKTENDKGLPYVIFSYGRNGKIENWEFNYENSSIIYSWE"
     gene            6390..6617
                     /locus_tag="PKW26_06990"
     CDS             6390..6617
                     /locus_tag="PKW26_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93347.1"
                     /translation="MKIRALFIHGSDSGSSFALALFLLSFIVYVYFPLALLADNKIKV
                     QKQKLEMLTHAIERDNKVYENCFEELLYETP"
     gene            6604..6957
                     /locus_tag="PKW26_06995"
     CDS             6604..6957
                     /locus_tag="PKW26_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93348.1"
                     /translation="MKLLEMIFVVAVICLFASSFFGGFALLEKEKLYLDFLKKYEAVV
                     FIDQDFKAKSKMGYFLSSESKDEWMLFIENYFSLESIRFFETSKDRKFCYDLEFFLSG
                     EKYTIKYAGRLDEKI"
     gene            6944..>7535
                     /locus_tag="PKW26_07000"
     CDS             6944..>7535
                     /locus_tag="PKW26_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93349.1"
                     /translation="MKKYNYMDDADYCVFEFEKNMLSFKELFFQKKWKSIIRSELEKM
                     YPSFDSFTLWQVKGLYSFFRKKILVIVVEPEVYARYRMKVKKTRILYKGKKYTFSLNS
                     IHIFLGIFSFLFLIALLFFFRFYTKERYREPVVNHSFENIFPEETKSKLLSVYTALEC
                     LKKESRGIDFEEFKWEIQKDGEEVFEIRIEDMFPEQI"
BASE COUNT         2462 a         1184 c         1471 g         2418 t
ORIGIN      
        1 aagctgatgc ggtcatatgt gcaggagatt ttgctgaggt tttcaaagaa gaaactggtt
       61 tacctgtgtt ggaaaaattg ataaaaaaac acgagacgat tttttctgtt ttggggaatt
      121 gtgacccacc ttctttttta gaaaaactag aatcctatga tgtctcggtg caaggcttga
      181 tcgcctcctt tgaggggctt acttttatag gcgccggggg agcaacacgt tttacagggc
      241 aaacaccaaa tgaaatttct gaggaagaaa tactcggaga cattgctctc gtgcaaaatg
      301 gagaagatgc tccttgggat aatctcgtta tgatcattca caaccctcct catgatacaa
      361 agctcgataa ggtttccatg ggccttcatg ttggttcaaa gaaaatccga gaagcagttg
      421 aagagataaa acccttggtt cttgtaagcg ggcatattca tgaatccttt gctatagata
      481 gtttgggaga tacgctttta ataaatcctg gttctcttgc tgagggtcgc tatgctattt
      541 tagaaataga gaaaaaaaac ggcgtctttg aagcaagggc tgagttaaaa gaaataattg
      601 tcccctaaag attttaatac aaaggcttga gactacgaca aaggcgaaag cccatgtaat
      661 tgaatgcgat gtctggatca aaagcatagc ggtcgcctgc ataaataaat gatgcatacg
      721 gactccaact gccaccacga ctgacgcgtt catagccaaa gtctggacct gtgtaacgat
      781 ctcttatatc aaaaggctgt acgtaatcgg caaaccagtc ccagcagtat tctaaaacat
      841 ttccgctcat atcgaagaga cctgcgccat ttgctcgccc gcttcctact tctgcatttt
      901 ttgaaaagag cgttcctgcg gtaccaactt tttctgtctt tgttgtattg ccacttgtcc
      961 atgcgatatc tgtctctaga acagaatcgt cgctctgtcc cttagcattt actgagccag
     1021 agggaaggtt gcctttttgg aatcctgttt ttgtttttcg agcagcccac tcccattcag
     1081 cttctgttgg aagtctatag ccatccgctg tccagtcgca gacgacaagg tctatttttg
     1141 cactatcagc agagtttcgt aaaatctctc cgtcaaaggt atagcaaggt gtatagccac
     1201 gtaattcgct tagggcattg caccatacaa gggcatctcg ccagcttatt gttgtgacag
     1261 gttcaaacct tccctcttct gtgggctcct ttcctcgctt tccttcagag ccttcttgac
     1321 caggattttg aaaaagatag cctctttcaa gtgcttgttg atatacctcg taccagagtg
     1381 cgtaggtggt ttcgtaggca tttatttcga agggggagag aatgagtttt actacactcg
     1441 attgcacact ttctcccaaa acaaagggag actccaagcc ttctatttca gatttacttg
     1501 caaaactata cattttaagg tgctcaggaa gttgagatag actttgtttt tcttcggttt
     1561 tatgcaacca acgccattca tttacagcgc tttgagaaaa aagagctgcg gcgatacaaa
     1621 taaaaaatat gctatagagg tgttttctct tcataaaaag agtatacatc gaattttata
     1681 ttaggtgaag tccttaaaaa taattaaatc ctctgtcaaa tactaaaaaa tcttgaatat
     1741 tttattattt ttaatttttt tttaaaaaaa ataaaaaaaa ctccttagaa aaaccaataa
     1801 caagatagga aaaaataaaa aggagttaaa actacgatgg taaaaaaagc gatacccttt
     1861 ctatgctgct ttttcgtgtt tatatcaatg ctttcttcga aaagctatga gtttatcgat
     1921 caaaacatag acgaggtgct atttgtgctt tctttagaag aaggcaaaac aattatagga
     1981 gacgatacag taggcggaaa agcgaccttt agggcagttg gagaaaattt ttccagcgtc
     2041 tttgatttgt ttttagcaca gcagcgcttt tatgttgaaa aaaaagatga tctttggctt
     2101 gtttcaaaga tgcgggtttt atgcgacgac gaaaatgttc tgcaagttga tgcctttgac
     2161 atacaaccac agcgcttatt tgagcagata agcagcgtgt cgggtgtacc tattttattt
     2221 gacattatac ccacacaaga aatttccgtg catgtgtcaa atctttctgt aaaagattgc
     2281 cttgtgctta ttttggcaag cttgggaaat tatactgtta cagaagagaa aaatatgctt
     2341 cgtatacaac gcgaagaacg gcagatgatc gaaaacaaaa actatggata ttgccgaatt
     2401 gaaaaaagtg caaagagaga aaatgccgag cctagtgcag aaaaccgttt tatcgaaggc
     2461 gattattttt ctgtggagat ttcccaagcg attggaaaaa atgttttaga ggaactttgt
     2521 gagcgtgcgg aaaaagaagt cgtctttctc gctccctgtg atgccttgat agaaacgata
     2581 cagctaaaag ataaaagctt tcaagaactt ctcgatttat tttgtatgca attatatgta
     2641 agtgcaatcg aaaaagatgg agtactctat tttgtagagg gacaagatac attattcaat
     2701 agcaaaaact ttggcaaaac gtgggatatg tacaagcttg attatcttaa gagtcaagag
     2761 tttagctcct ttttgcaaaa tcgatttgaa aatcttcgca tgtatactat ttcttcttcg
     2821 cttctttttg ttcttgcaaa tgataaaata cataaagaaa ttcaatcatt gattcagata
     2881 gtggatatcc aaaaacagcc ctttattgtt tctttgcagt ttatcaaagc agaagaattt
     2941 ttcgcgcatt taccgccagg atatacagct gatgaatttt atagaatggg acatgatggg
     3001 gaactctttt atctaggtac agaaaaaaaa tacgagtcct tattggggct tgtggaaaaa
     3061 atagatttgc cggcgaaaca aattctctac gatttgttgg ttcttcaagt gcaagattca
     3121 agtagttttt cttgggagcc atctttgtcc gcaaaaacaa tttcggctgt cgagcgtccc
     3181 tttgtaagtg ttgatttagg ttcggtttta gatttgcagt tcaatgtctt atctgccttt
     3241 ggtataaatt ttgcagttaa cttacaaaca gctataaatg agagcaaggc aaaaatattt
     3301 gctgatacga gtttacatgg cttatcggga actcccattc atttccaaaa tacaaatacc
     3361 tatagatata aagatattgc tataaatcca gaaacgggaa agccaatgta cacgggaatt
     3421 acaagggaaa ttgtttcagg tttatttcta gaagttgaag gttgggtttc aggaagcggg
     3481 atgattacaa caaaggtaaa ggcgactatt tctaaacgtg gtgcagatgt ttctttgtcg
     3541 gctggaaatc ctccaccgac atatgaaaaa ataattacaa cagaggttcg atctaaaagc
     3601 ggagagacag ttattttaag tgggcttata caagacgatt ctgttttaat tgaacagcgc
     3661 attccccttg tatcaaaaat tcccattata ggaaaattat ttagctctct aataaatacg
     3721 cgtgaaaaaa cagaaatgct tatttatctt atacccaagt tagtaaatga tgatgaagaa
     3781 aataaactag aaattcgaga taaatatcaa gaacggatgg acgacattac aaaaaacata
     3841 tttcattttt taaaggagga atgtggatat gagttttaat ttatcttcaa agttttgcgc
     3901 ctataataaa gtggtgcaac tggaaaattc tgaaatgcaa tgtgtgattg gtctcagtca
     3961 agaaaatgat gagataaaaa atattattgc aaaaaacttt aatcaaaaaa gaaggtcaga
     4021 aagacaaagg cttttgttta gaaaaatttc tactgaagag tgtttgcgaa aattatcgaa
     4081 agagttttca aatgcattgt ttcttaaaaa agttgaggag aaggaaaaag agaaaagtca
     4141 tttcgatgat gtccctataa aaaatatttt gaattcgatg attattgaat gccttgaaaa
     4201 taaaggaagt gatattcatt tgcagagatg ggataatttt tatgaactgc gctttcgcat
     4261 taacggtacg atgaaaacgc aatcgaaaat aaatgctgaa atcggcgatg cgcttatgca
     4321 acgtgtgaag gttttatcaa agttggattt ggctgaaaaa cgaaaatgcc aggatggtcg
     4381 ttttgacttt ctaaaaggcg attatgaagc tgagttacgt gtctccgtta tcccttgctt
     4441 ttacggtgaa tccttagttt tacgaatatt ggatcggaaa aaaaatatag aaaatttttc
     4501 tagtcttggt tttactggag aacaagaaaa aatgctcgat gaaattgtga aaagtacaag
     4561 tggtttggtg cttatttgcg gaccgactgg ttctggtaaa acgacaagca tgagtagtat
     4621 actcggtgag attcaaaagc aaaacaaaaa aataattagc ttagaagatc cagtcgaata
     4681 cattatccca aatgttacac aggtgcagat caataaaaat ctcggcttag attttcccac
     4741 attacttcga agtgttattc gacaagaccc tgacattgtt gcaattggtg aaataagaga
     4801 tagtgcaaca gcaaagattg cagtacaaac agcgcttact gggcatttag ttttggctac
     4861 tcttcatact gattcagctc gagatagttt tttccgttta acggatatgg gcgttcctgc
     4921 gtatttactt tcaagtgttt tgaaatgggt tatttgccaa gaactcgtcg tcctgccaaa
     4981 ctcaagaaag ctcaatgctt ccataacata ttgtgattca gattttcttt cagaattgaa
     5041 agaggagtgc agatatggca gctaatttgt tttacatctt ttgctcgaac cttttaggtt
     5101 ttttgcaggg aaaattctcc ttgcaagaat ccttggaaat aatgatttca catgaggcaa
     5161 caggtaaaaa aataaaaaac tttacatctt atatattaag tgagttagaa aaaggttccc
     5221 cgtttacaac tattttaatt gcaaatccat atatcctgat aaaaaaacaa taccagcttt
     5281 tattttatag cgaagaaaaa acaggagatt ataaaaaaag tctagattat gtatgtaaga
     5341 gtgaaaaaga aaaaaatgaa actatagaag aagttataaa actttcttta tatcctttat
     5401 ttattatttt gctttgcata tttggttcta ttgcttttat tttctacagc aaagactttc
     5461 ccgtttatgt acaacaagca tctgttttta gcggaattgc atatgcaaat atatttttag
     5521 ttttctttct tttgcttttt gcattttctt tttatatgct acataagaaa ccaattaaaa
     5581 atattttttt aatctgtctc gatttttttc ttgcacaatc ttttgatttt acaacagcct
     5641 tagaatatgc aagctatgtt tttgatgaca aaaaaaacat acggcaaatt gttttggact
     5701 ctttatctgg attatcactt ggattagaac tttcgactgt ctttgacaaa acgggtttat
     5761 ttacaaaatc agaaataaaa accttggacc tatatatttt caatggaaat ctacgcggcg
     5821 ggatagatat tgttttaaaa gagcttgaga gaaaagctca aaaaaacaaa gacatcttta
     5881 tatccttggt tgagccaagc ttaataatct gtgccggaat ttatttttta ctaatagtta
     5941 tacattcact cttaccctat ttgacttttt atggaggtct tttatgaaaa aaaaacatga
     6001 tgaagccttt acttttgcag aaacaattgc atcccttgca attattctca ttcttagttg
     6061 tagcgttggt tttgctgcct ttaagtttat cgatagggca aaagaagttt ctgtacaaac
     6121 gcaaatctta cagtttaaaa ctgcattaaa tgcatattat ttggactgcg gattttttcc
     6181 caacgaggca cagggcttag aagctttatg ggaaaaaccg tttttagaac cagtgagttc
     6241 tgaatggaag ggaccatata cagataaaaa aataccaaag gatccttggg ggaattttta
     6301 tgtgtataaa actgagaacg acaaagggct tccatatgta attttttcat atggaagaaa
     6361 tggtaaaatt gagaactggg agtttaatta tgaaaattcg agcattattt attcatggga
     6421 gtgattcagg ctcgagtttt gctcttgcat tatttctctt gagttttatt gtttatgtat
     6481 attttcctct tgcgctcctt gcagacaata aaataaaagt gcaaaaacaa aaactggaga
     6541 tgctcactca tgcaatagag agagacaata aagtctatga aaattgtttt gaggaattgc
     6601 tctatgaaac tccttgagat gatttttgtc gttgcggtga tctgtttatt cgcatcaagt
     6661 ttttttggag gttttgcttt gttagaaaaa gaaaaattgt atttggattt tctaaaaaaa
     6721 tatgaggcag tcgtttttat agatcaagat tttaaagcaa aaagtaaaat gggatatttt
     6781 ttgtcgtcag aatcaaagga tgaatggatg ctttttatag aaaactattt ttctttggaa
     6841 agtattcgtt tttttgaaac atctaaggac agaaagtttt gctatgacct agaatttttt
     6901 ctgagcggcg agaaatatac aataaaatat gcaggaagat tagatgaaaa aatataatta
     6961 tatggacgat gcagattatt gcgtttttga atttgaaaaa aacatgctta gttttaaaga
     7021 gctctttttt caaaaaaaat ggaagtcaat aataagaagt gaactagaaa agatgtatcc
     7081 gagttttgac agctttactt tgtggcaagt aaaaggcttg tactcttttt ttcgaaaaaa
     7141 aatcttagtt attgttgttg agccagaagt ttatgcgagg tataggatga aagtaaaaaa
     7201 aacaaggatt ttatataagg gaaaaaaata taccttttca ctgaactcta ttcatatttt
     7261 tttgggaatt ttcagttttc tttttctaat agctcttttg tttttctttc gattttatac
     7321 caaagaaaga tatagagagc cagttgtcaa tcatagcttt gaaaatattt ttcctgagga
     7381 aacaaaatca aaattgcttt ctgtttatac cgcattagag tgcttaaaaa aagaaagtag
     7441 aggcatagac tttgaagagt ttaagtggga aatacagaag gatggggaag aagtctttga
     7501 gatacgtata gaagatatgt ttcctgaaca gataa
//
LOCUS       DAOSSW010000082         6887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_10192_length_6887_cov_7.746048, whole genome shotgun sequence.
ACCESSION   DAOSSW010000082 DAOSSW010000000
VERSION     DAOSSW010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10192_length_6887_cov_7.746048"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PKW26_07005"
     CDS             <1..700
                     /locus_tag="PKW26_07005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOQ93350.1"
                     /translation="ISGTMHNVFSVGQTLTELGFKILNVITWQKTNPPPNFSCRYFTH
                     STEQIIWARKNEKTPHYYNYELMKQLNGNKQMKDVWSLPAIAPWEKSCGKHPTQKPLA
                     VLTRLILASTKPKAWILDPFTGSSTTGIAANLLNRRFLGIDQEEEFLRMSKNRKIEIE
                     NDDIFASYKQKIRGFNSKKELTLFLSEEPIEEYDSELKLGNKKSNKKKDLRELKGKIR
                     FADDYDYKKMRTGK"
     gene            897..2186
                     /locus_tag="PKW26_07010"
     CDS             897..2186
                     /locus_tag="PKW26_07010"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOQ93351.1"
                     /translation="MARGLREKKMAGDYNPYEEMLKVLDDAAQKLGLKEDDYIKLKYP
                     EKELKVAIPVTMDDGSTKVFEGFRVQHSSVRGPYKGGIRYHQNVDVNEVKALAALMSL
                     KCAVVNIPYGGGKGGVTVDPTKLSKAEIERLTRSMVTQFFPIIGPTKDIPAPDVNTNA
                     EVMAWFVDTYSTLKGELSPGVVTGKPIALGGSLGRAEATGRGIMFATREIAKKKGLSL
                     KGARVAVQGAGNVGGVAAKLIHREGGIVVAISDVSGGIYKEDGLDIDEIITFLTAERG
                     RLLKDYDVPGLKRISNDDILTQDVDILVPAALENQITEKNADKIRAKIIVEGANGPTT
                     SAADAILDKRGISLVPDILANAGGVVVSYFEWVQNLQNFYWSEAKVNESLEEIMVNAF
                     ESVWETAEKHGVSLRVAAYMVSVERIVVASKMKGMAN"
     gene            2190..4007
                     /locus_tag="PKW26_07015"
     CDS             2190..4007
                     /locus_tag="PKW26_07015"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOQ93352.1"
                     /translation="MKASETLIATLREVPQDAVIASHQLMLRSGLLRRLGNGLFAYLP
                     LGLKAFRKVEAIIREEMDAIGALEFRPSVIVPGELWRESGRWETMGEGMLRVKNRVNQ
                     ELVVSPTAEEAFTALIRDELSSYKQLPITGYQINTKYRDEIRPRYGVMRGREFTMKDA
                     YSYHANQESLNEVYEKFASAYRKIFSRLSLTVIPVKADTGAMGGTGSEEFMVESPVGD
                     DTLILCASCGYAANTEKASCASDADMSLVKTDKAVEEIDTPNVKTIEELSAFLDTKPQ
                     TFIKTLIYKALNSELDLTKAPGSASLKRVTETGRAAPFYPESFFAVCIRGDLDINEAK
                     LAALVKASELELASDEDVERFTQAPVGFAGPVGLDSLPIIVDNSVLAMHDAVTGGLKK
                     DVHLLHVEPSRDFSFFLSGDIRTVVAGDICINCGKTYHSTKGNELGHIFKLGDKYTKS
                     MGVSFLDENGKLQTPTMGCYGIGVDRSLASIIEEHHDENGIVWPMNIAPYQVVIVPIK
                     YNGQMKEIADNLYEGLRKEGIEVLLDDRDERPGVKFKDMDLIGIPVRVLVGEKNLPNV
                     EIKERNKSSADLVLVEEAKDFVTKLVKEALAKHAHSSEL"
     gene            4074..5144
                     /locus_tag="PKW26_07020"
     CDS             4074..5144
                     /locus_tag="PKW26_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93353.1"
                     /translation="MKKVVCCSVLFILLFCLRLFALPGIDEWMPTASGEYVYYRDYTF
                     TDESYIGFLQYDAGTYAVRYYSLGNTSDKKNPIPPKDIQILLTMKKDSAFVELTGEKI
                     IGELEAEDVDIVNYLHDLVYELNARRKKMNGKDFSKALVSSEIYEQFGGEVTMTYKGY
                     IPLYSLDAIRSEKGDLLFQAVTIGTLLDSSDTSFADFAGFKNLTSLKRVADKKANLDE
                     FWKTNDNIVYSINNKALYMVNTFTFEESYFSNLPYSLEDYFIRNFLTSTEGDYIYLPF
                     VSIQKEKGERKPYLLLTSTIMRTEESMRITKTKDAFQKDFKVLIRENENSYWLCTLTM
                     ENDYYNLNKSYFDNYLKTFVLP"
     gene            complement(5162..5992)
                     /locus_tag="PKW26_07025"
     CDS             complement(5162..5992)
                     /locus_tag="PKW26_07025"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOQ93354.1"
                     /translation="MAKKSNKNTKLAFFFWLIFATIIAIAFFMARGRIANVLEETGFI
                     NEVLGDKKETVVDILQPQKKETVIEIIPSNESETKSSQQNGKETSTTSTEPKIETITP
                     IIAQATDQKEENLSLSKTEKKDTTKQDNKTEAAKPSTDLQLCFIAIDSDGTVVRREIV
                     RTVDKTNTPLTASLKALLAGPSLNEMEKGTMSLIPEGTKLLSASITDRVAYLSFSDEF
                     NINKYGVEGYIGQLMQIVYTATAFSTIDSVQFLINGQKQNYLAEGVWIGSPLPRTTFK
                     "
     gene            complement(6071..6886)
                     /locus_tag="PKW26_07030"
     CDS             complement(6071..6886)
                     /locus_tag="PKW26_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOQ93355.1"
                     /translation="MKCLVCFHPITLALFATLFTWGMTALGASLVFFFKTINQRALNM
                     MLGFASGVMIAASFWSLLDPAIQMSEGGPIPPWAVAAIGFVLGAAFLWLADRLMPHIH
                     FSSKTGEEEGIHTNLKRSILLVFSITLHNIPEGLAVGVAFGSLTGVITPEATLPAIAV
                     AIGIGIQNFPEGAAVSIPLRREGLSRFKSFMYGQASGIVEPIAGVLGAVLVTQVTSIL
                     PYALAFAAGAMIYVVIEELIPTAQEGDHQSTDLATVGAILGFTAMMMLDVAFG"
BASE COUNT         2074 a         1191 c         1581 g         2041 t
ORIGIN      
        1 gataagcgga acaatgcata atgttttttc agtcggacaa actttaacag agttaggatt
       61 taaaattctt aatgtaatta cctggcaaaa aacaaatcct cctccaaatt tttcttgtcg
      121 atattttacg cattcaacag aacaaattat ttgggcgaga aaaaacgaaa aaactcctca
      181 ttattataat tatgaattga tgaaacagtt gaatgggaat aagcaaatga aagatgtttg
      241 gtcattacct gctatcgctc cttgggaaaa gtcttgtggt aaacatccca cacaaaaacc
      301 tctcgctgtt ttaacgcgat tgatattggc atcgactaag cctaaagcgt ggattttaga
      361 tccctttaca ggtagttcta caacaggaat tgctgcaaat ttattgaaca ggagatttct
      421 aggtatagat caagaagaag aatttttgcg tatgagtaaa aatcgtaaaa ttgaaataga
      481 aaatgatgat atttttgcga gttataaaca aaaaatacgt gggtttaatt caaaaaaaga
      541 attaacattg tttttgtctg aagagccaat agaagaatat gacagcgaac ttaaacttgg
      601 gaataaaaaa agcaacaaaa agaaggattt aagagaactc aaaggtaaaa ttagatttgc
      661 tgatgattat gactataaaa aaatgagaac tggtaaatga ttttagtaga cagttctgtt
      721 ttagttgatt atttaaaggg ttataaagaa tcttcgctta tatgaaaacc tggcttcttc
      781 ttaatttttc ctctctcgtt gacagtgttt tatcaaatta ttataattag ttattctata
      841 ttaattaagc aaattctatt cttatgagta atcaaacaca aatttcacta ctgataatgg
      901 ctagaggttt gcgggagaaa aaaatggcag gtgattacaa tccctacgaa gagatgttga
      961 aggttctaga tgatgcggca caaaaacttg gcttaaaaga agatgattat attaagctca
     1021 agtatccaga aaaagaatta aaggttgcta ttccggtaac gatggatgat ggctcgacta
     1081 aggtttttga gggtttccgt gtgcaacatt caagtgtgcg tggtccttac aagggtggta
     1141 ttcgctatca tcaaaatgta gatgtcaatg aagttaaggc gcttgcggct cttatgagtt
     1201 taaaatgtgc tgttgtaaat attccttacg gtggtggtaa gggtggggta actgttgacc
     1261 caaccaagct gtcaaaggct gagatcgagc gtttgacacg aagcatggtt acacaatttt
     1321 tccctattat tggtcctaca aaggatattc ctgcgccgga tgtaaatacg aatgcggaag
     1381 taatggcttg gtttgttgat acatacagta cgctcaaggg tgagttaagt cctggtgttg
     1441 ttactggcaa gcctattgcc ctcggtggtt ctcttggaag agctgaggca acgggtcgcg
     1501 gtattatgtt tgcaacaagg gagattgcaa agaaaaaggg gctttctctc aagggtgctc
     1561 gtgttgcggt tcaaggtgcg ggcaatgttg gcggtgttgc tgcaaagctt attcacagag
     1621 agggcggcat tgttgttgca atttctgatg tatctggtgg aatctacaaa gaagacgggc
     1681 ttgatattga cgaaattatt acgttcttaa ctgcagaacg tggtaggctt ctcaaagact
     1741 atgatgtgcc aggcttaaag cgtatttcca acgacgatat tttaacgcaa gatgttgata
     1801 ttcttgtgcc ggcggctctt gaaaatcaaa ttaccgaaaa aaatgctgac aagatacgag
     1861 caaagattat cgtagaaggt gcaaatggtc caacgacttc tgcagctgat gcaattctcg
     1921 acaaacgagg cattagttta gttcccgata ttttggcaaa tgctggtggt gttgttgtat
     1981 cctattttga atgggtacaa aatcttcaaa acttttactg gagtgaagct aaggtcaatg
     2041 aaagcttgga agaaataatg gtaaatgcgt ttgaaagtgt atgggaaaca gcagaaaaac
     2101 atggtgtttc tttacgtgtt gcagcctaca tggtctctgt tgagcgaatt gtagtcgcaa
     2161 gtaaaatgaa aggaatggca aactaagata tgaaagcaag tgaaactcta attgcaacct
     2221 tacgagaggt tcctcaagat gctgttatag caagtcatca actaatgctc cgttctggtt
     2281 tacttcgtag gcttgggaac gggctttttg cctacctacc gctcggctta aaagcctttc
     2341 gaaaggttga ggcaattatc cgtgaggaga tggatgccat cggcgcttta gagtttcgcc
     2401 cttctgttat tgttcccggt gagctttggc gtgagtctgg tcgctgggaa acaatgggtg
     2461 agggaatgct tcgcgtaaaa aaccgcgtga atcaagagct tgtggtaagc cctaccgcag
     2521 aggaggcatt tacagccctt attcgcgatg agctttcttc ctacaagcaa cttcctataa
     2581 ctggctatca aataaataca aagtatcggg atgaaattcg tcctcgatac ggggtgatgc
     2641 gaggcaggga gtttaccatg aaggatgctt attcctatca tgcaaatcaa gaaagcttga
     2701 atgaggtata cgaaaaattt gcttctgcct atcgaaagat tttcagccgt ttatctttaa
     2761 cggttattcc tgtgaaggct gacactggtg cgatgggtgg aactggttct gaagagttca
     2821 tggttgaaag ccccgtcggt gatgataccc tcattctttg cgcctcatgt ggctatgcag
     2881 ccaatactga aaaagcaagt tgtgcttccg atgcagatat gagtcttgta aaaaccgata
     2941 aagctgtcga agaaattgat acaccaaatg taaaaacaat cgaagagctt tctgccttct
     3001 tggatacaaa gccacagacc tttattaaaa cgcttattta taaggctttg aactcagagc
     3061 tcgatttaac aaaggctcct ggttctgctt ctttgaagcg agttaccgaa acaggtcggg
     3121 ctgcaccctt ttatcctgaa agtttttttg ctgtttgtat tcgaggtgac cttgatataa
     3181 acgaagcaaa gctcgccgct ttagtaaaag ctagtgagct tgagcttgcc tccgatgaag
     3241 atgttgagcg ttttacacag gcgcctgttg gttttgcagg tcccgttggc ttagatagtc
     3301 ttcctatcat tgttgataat tctgtgcttg cgatgcatga tgcggtaaca ggtggcttaa
     3361 aaaaagatgt gcaccttctg cacgtagaac cgagtagaga tttttctttc tttttaagcg
     3421 gtgacatacg aaccgttgtt gcaggtgata tttgtattaa ctgtgggaaa acctaccata
     3481 gtaccaaggg aaatgaactc ggtcatattt ttaaacttgg cgataaatat acaaagtcga
     3541 tgggagttag cttccttgat gaaaatggaa agctacaaac gccaacgatg ggttgttacg
     3601 gaattggtgt tgaccgatcg cttgcttcta tcatcgaaga acatcatgac gaaaatggta
     3661 ttgtatggcc gatgaatatt gcgccctatc aagtggttat tgttccaata aaatacaatg
     3721 ggcaaatgaa agaaattgct gataacttat acgagggctt acggaaagaa ggcatcgaag
     3781 tgttgcttga cgatagggac gagcgacccg gagtaaagtt taaggacatg gacttaatag
     3841 ggattcctgt tcgcgttttg gtgggagaaa aaaatctacc aaatgtcgaa ataaaagaac
     3901 gtaacaaaag cagcgctgat cttgttttag tagaagaagc aaaagatttt gtaacaaagc
     3961 ttgtgaaaga ggctcttgca aaacatgctc actcgagcga attgtaaaaa ctgaggtgca
     4021 agattttgta caaaaaggct cttgttaaaa gagctttttt tattggagga tgtatgaaaa
     4081 aagtagtttg ttgttctgtg ctttttattt tgctgttttg cctaaggtta tttgccttgc
     4141 caggaattga tgagtggatg cctactgctt ctggggaata cgtatattat cgggactaca
     4201 cttttacgga cgaaagctat atcggctttt tgcagtacga tgcgggtacc tatgctgttc
     4261 gttattattc acttggaaat acgagcgata aaaaaaatcc cattcctcca aaagatattc
     4321 aaatccttct tacaatgaaa aaagattctg cctttgtaga gttaactggt gaaaaaataa
     4381 ttggagagct cgaagcagaa gatgtcgata tcgtaaatta ccttcatgac ctcgtgtatg
     4441 agcttaatgc acgacgtaaa aaaatgaacg ggaaagattt ttcaaaagcg cttgtatcca
     4501 gtgaaatcta tgagcagttt ggtggagaag ttacaatgac atacaagggc tatatccctc
     4561 tgtatagttt ggacgcgata cgaagtgaaa agggagatct tctctttcaa gcggtaacga
     4621 ttggtacctt gctcgattca agcgacactt cgtttgcgga ctttgcaggt tttaaaaacc
     4681 tcacttcact aaaacgagtt gcagataaaa aagcaaacct cgatgagttt tggaaaacaa
     4741 atgataacat tgtgtacagc ataaacaaca aggcgcttta tatggtaaat acctttacat
     4801 ttgaagagtc gtatttttca aatctgccgt atagcttgga agattatttt atacgaaact
     4861 ttcttacatc aactgaaggg gattatattt acctaccatt tgtttccata caaaaagaaa
     4921 aaggggagcg aaagccctat ctgcttttaa caagtaccat aatgcgaaca gaagaaagca
     4981 tgcggattac aaaaacaaag gatgccttcc aaaaagactt taaagtgctc attcgagaaa
     5041 atgaaaattc ctattggctt tgtaccttaa ctatggaaaa tgactactat aatttaaata
     5101 aatcctattt tgataattac ttaaaaacct ttgtcttgcc gtaatggcgt ttcacaaatc
     5161 tttatttaaa ggtagtcctc ggaagggggc tacctatcca aactccttcg gcaagatagt
     5221 tttgcttttg gccgtttatt aaaaactgca cagagtcgat tgttgaaaag gcggttgcgg
     5281 tatagacaat ttgcatgagc tgaccgatat agccttcaac gccatattta ttgatattga
     5341 attcgtcgct aaagcttagg tatgctaccc tgtcggtgat gcttgcggat aaaagttttg
     5401 tcccttcggg tataagcgac atggtgcctt tttccatttc gtttagagaa ggacctgcga
     5461 gaagtgcttt taaagaagcg gtgagaggag tgtttgtttt atcgacagta cgaacaattt
     5521 cgcgtcttac gactgttcca tcgctatcaa tggcaataaa acaaagttgt aaatcggtgc
     5581 taggttttgc tgcttctgtt ttattgtctt gttttgtggt atcttttttc tcagttttgc
     5641 taaggcttaa attttcttct ttttgatcgg tggcttgggc tataatcggc gtgattgttt
     5701 ctatttttgg ttcggtggaa gttgtggaag tttctttacc attttgttgg gagctttttg
     5761 tttcgctctc attcgaggga ataatttcga ttaccgtttc ctttttttgc ggctgcaaaa
     5821 tatctacaac agtttctttt ttatcaccaa gaacttcgtt aataaaacct gtttcttcaa
     5881 gtacattggc aattcttcca cgtgccataa aaaaggcgat agcaataatt gttgcaaaaa
     5941 taagccaaaa gaaaaaagcg agttttgtgt ttttattcga ttttttagcc atagaaaaag
     6001 tatagtatag aaagtgaaaa aagaaaatag cttgataaaa aaaagcccac gtgtttgtga
     6061 gcttttttct ttagccaaag gcaacgtcga gcatcatcat agcggtaaag ccgagaattg
     6121 ccccgacggt tgcaaggtct gtgctctggt gatctccttc ctgagcggtt gggatgagtt
     6181 cttctataac aacgtaaatc atagcaccgg cagcgaaggc gagagcgtag ggtaagatgg
     6241 aggtaacttg tgtaacgagg acagctccca aaacgcctgc aatgggttca acgatgccag
     6301 aagcttgtcc gtacataaag cttttgaagc gggaaagacc ctctcgtctg agtggaatgg
     6361 aaacagctgc tccttcggga aagttttgta ttcctatgcc gatggcaact gcaattgcgg
     6421 ggagggttgc ctctggtgta atgacaccag ttaaagatcc aaaggcgact ccgacggcta
     6481 agccttcggg gatattgtga agtgttatag aaaaaacgag gaggatgctt cgctttaagt
     6541 tggtatggat gccttcttct tcaccagttt ttgaggaaaa gtgaatgtgt ggcatgagtc
     6601 ggtctgcaag ccaaagaaag gcagctccga gcacaaagcc aattgcggcg acagcccatg
     6661 gcggaatggg tcctccttct gacatttgta ttgcggggtc taagagagac cagaagcttg
     6721 cagcaatcat aacgcccgat gcaaagccga gcatcatgtt aagcgctcgt tgatttattg
     6781 ttttaaagaa aaaaactaaa cttgcgccaa gggcggtcat tccccaggta aaaagtgttg
     6841 caaaaagcgc tagggttatc gggtggaaac aaactaaaca tttcatg
//
LOCUS       DAOSSW010000083         6881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_10205_length_6881_cov_6.489452, whole genome shotgun sequence.
ACCESSION   DAOSSW010000083 DAOSSW010000000
VERSION     DAOSSW010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10205_length_6881_cov_6.489452"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PKW26_07035"
     CDS             <1..762
                     /locus_tag="PKW26_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93356.1"
                     /translation="MVLGDSFSLYTALDVKRDTYTLRQLILSFFPELSEKNQEALLLK
                     ISTMYIGFSLDTKEFSLVVEGNFPALLKSQLNKKNGWEKKRYSPESVGLAQNFFLHTD
                     GIALAFVHKNLLFISNNQIEAMLERFSLTNRIAIPGGIEGISNEDYNYLKLRQALQEE
                     TILLYIKEPKTLLSQFLPFDSDLALDYMLVYAEEKERDYSLTIDFVFTDTRAVRPALL
                     LFKIAFYNYDVFIEQIAENQVQVKNISFNLWDGIQ"
     gene            780..>1615
                     /locus_tag="PKW26_07040"
     CDS             780..>1615
                     /locus_tag="PKW26_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOQ93357.1"
                     /translation="MDSKVILKAESLDGYLTEKDLSYLKEMDILYERVMKSFQAIAQG
                     GFSNTIAQEEKKVIELFNEMGHIMQTICTEVPAMQVYSFITTEESHAEASRVIAKLRD
                     IRTGHQEFLYYTQRAYEMLFRLAYSGYHSDNKNYLVVKTPVTAPVQNYSVHKIPDIDH
                     KIENTVMCVMLRGALLPSMIMSKEIEEYSSHGYVTPFALFKIKRDDLKKESNMEYILD
                     LKNSFFNLEDLHGKDLIFADPMNATGGSLVTVIKYLQDQGVKPKSIKFFNVIAALKGS
                     LRV"
     assembly_gap    1616..1715
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1929..2276
                     /locus_tag="PKW26_07045"
     CDS             1929..2276
                     /locus_tag="PKW26_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ93358.1"
                     /translation="MYKIGLIFVEQKKYDKAENVFDDLLKKDTKNATILSQLAFVKSK
                     QKKFLEAIYLYEDILLMYPMDQTTKKNLALVYWYNGDTEKARSIQKELIDENPLDTSL
                     KDLLKEDEGNKAG"
     gene            complement(2299..3645)
                     /locus_tag="PKW26_07050"
     CDS             complement(2299..3645)
                     /locus_tag="PKW26_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93359.1"
                     /translation="MNRSTTIFIAFFAITMYLFAIAFFTFKMISYSQQGRENIIVQCT
                     DLTQKAFDFIEEFDGINDNFMQGFIAVLDMFPNIALVLVETEHNGFFSYPKNTSFLSQ
                     DDNSKNILKTQSPLISVYSSSLLHNNEPITLTFALYHVSPYLIHDLAKKSFFILFLTT
                     LFVFLTIIINKILQKNNRTESDSFPTSEIAFQDSQNSNFYNDFQKNDFDLDFDFDYKT
                     DTDAFLHNDPISDSEFALDIEKKDSIATVPLEEQVKDQVKKDSFLDRENKKQYAKFQI
                     QGKRISDPLGLFSEKTGFGWESYLEPRLDSELIRASSSEQDLALVLIKIAGIEHYPAY
                     TEFISEVLLEAFKFRDLIFEYGQDGFACIYQDLNVEKALTLAEELYIALDEILKNCDV
                     KIAIGISTRSLRIIPGTRILKEAQQAIDKALTEKEDPIIAFKANPDKYKDYVYNTL"
     gene            complement(3704..5275)
                     /locus_tag="PKW26_07055"
     CDS             complement(3704..5275)
                     /locus_tag="PKW26_07055"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOQ93360.1"
                     /translation="MYLIQQISSIQINSTRDKPKVNLRFTTSKNNQKICLWNTQFIHS
                     SYNPIIEAERFVKTLSFSGEPRYIIFIAPFGGHSYSFLKKRFPNARFIAIEFFDHKAF
                     REIPWDFYFTGTSSKLGNELFKIIGEEGSLFTEVIIWPTSERLFPEECQKTLEILRIF
                     FNTSRDVLGTRLFFTKKWNTNTFRNIKYAKKIIPAFRIEKPVCIIASGPSLTKTIDFL
                     QKHKDRMFLIALSSSMPVLSKYKIEPNVCFTTDGGFWAKMHLEKYGEQFKNTIFIASL
                     EAALPYNILHKNFLHFIDYGDGLSHQVIKKTLLSSQAALRCGTVSGTALGFSQNSSEK
                     DIFFLGLDLAHTKNYPHSQPNALENYNAPRDSRIKPKEDRITKAAYNGNGSLALYENW
                     FKNIHASKNKIYRIKAENKDFSNSFPAIKDISENEAVQILLERQESPSPEGKKIFQTI
                     DTKGLESYLENTIKLLSTLEFEAQPFSAEINELYREISLKEFLAFQKKQTKTSFLELK
                     ENTVSFLTKSLLYLQ"
     gene            complement(5223..6200)
                     /locus_tag="PKW26_07060"
     CDS             complement(5223..6200)
                     /locus_tag="PKW26_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93361.1"
                     /translation="MGLLQKVEAQPKKPSQKKTGGLISKAEKLAFAKSKPIFPKNSLL
                     LDESHVFFKDFSETHEFVFSGILKKEEKQYKIIYAFNLTKMKLFDSSKDFWDGTLAKK
                     NTWNSFSCNDFAAFYQFFPTELNENLSLYFKEYNTEDNSYIYFFSSNNKSRQHNTLLI
                     NDRNIFDFIQLIDRLFISIDALKPNKPIISKNVFQQGLAIYSFAHFIQLENKILEKNE
                     NISESGLEKIKELVLILCNTVLPKPGFSFLSQNSCILVVFSNQNIDIEMLLILLKNFF
                     SDYLHSSTVSAFEITKYFSLNDLDKIDAFFPNNVFNSTNFIDSNQFNAR"
     gene            complement(6201..>6881)
                     /locus_tag="PKW26_07065"
     CDS             complement(6201..>6881)
                     /locus_tag="PKW26_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93362.1"
                     /translation="DDAEISLDDDELVEEFFEENLIEPSEEELDINTIVANNEELIDT
                     ITIDSSELVVPSADSDLLEVPVDQDNDLENPEINAIADFEDELLLDVEEEEEEEEEEE
                     EEEEEEDLSEIIPEAIEPDRDTEIDENSYNEPTDEVFNSEQWGFDDDASPSLENEAKS
                     IPATEPPATNSITKNIQDKQMQEDIKSVLVYMDQLLENLPEDKIEEFAKSENFVVYKK
                     LFTELGLV"
BASE COUNT         2175 a         1051 c         1206 g         2349 t
ORIGIN      
        1 atggttctcg gggattcttt ttctctctat acagccctcg atgtgaaacg agatacatat
       61 actcttcgac agctcatact ctcttttttt cctgaacttt cagaaaaaaa tcaagaagct
      121 cttttattaa aaataagtac tatgtatatc ggtttttctt tagacacaaa agaattttcc
      181 cttgttgttg aaggcaattt tcccgcctta cttaaaagcc aactcaataa aaaaaatggc
      241 tgggaaaaaa aacgctattc tcctgaaagt gttggactag cacaaaattt tttcttacat
      301 acagatggca ttgcattagc cttcgttcat aaaaatctct tatttatttc aaataaccag
      361 atagaggcaa tgctcgagcg tttttctctc acaaatcgta tagctattcc tggaggaata
      421 gagggtattt cgaacgaaga ttacaattat ctcaagctac ggcaggcgct tcaagaagaa
      481 actatactct tgtatataaa agagcctaaa acacttttaa gccagttttt accctttgat
      541 agcgatttag cccttgatta tatgctcgtc tacgcagaag aaaaagaaag agactatagc
      601 ttaacaattg attttgtttt tacagataca agggctgtga gacccgcgct tttattgttt
      661 aagattgctt tttataatta tgatgttttt attgaacaaa tagcagaaaa tcaagtgcaa
      721 gtaaaaaata ttagttttaa tctatgggat ggtattcaat aatataaagg aaggtatata
      781 tggattcaaa ggttattcta aaagctgaat cactcgatgg ttacttaaca gaaaaagatt
      841 tgtcgtatct gaaagagatg gatattctct atgaaagagt tatgaaatct tttcaggcaa
      901 ttgcccaggg cggttttagc aatactattg cgcaagaaga aaaaaaagtc atagaactct
      961 tcaatgaaat gggtcatatt atgcaaacga tttgcacgga agttcctgcg atgcaagtgt
     1021 attcctttat aacaactgaa gaaagtcatg cggaagcttc tcgcgtcatt gcaaaacttc
     1081 gagatattcg aaccggacat caagaatttt tatactatac ccaaagagcc tacgaaatgc
     1141 tttttcgcct tgcctattct ggttaccata gcgataataa aaattacctt gtggtaaaaa
     1201 cacctgtaac agctcctgtt caaaactatt ctgtgcacaa gattccagat atcgatcata
     1261 aaatagaaaa tacagttatg tgtgtaatgt tgcgcggtgc tttgcttccg tccatgatta
     1321 tgtcaaaaga aattgaagaa tattcttctc atggctatgt tactcccttt gccttgttta
     1381 aaattaaacg tgatgactta aaaaaagaat ccaacatgga gtatattcta gatttgaaaa
     1441 attctttttt taatttggaa gatttacatg ggaaagattt aatctttgct gacccaatga
     1501 atgcaactgg aggctctctt gtaactgtta taaaatacct tcaagaccaa ggggtaaaac
     1561 caaagtccat taagtttttt aacgtgatag cagcattgaa gggaagtttg cgtgtnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncattt gaaaaagaaa cagatagttc
     1741 ctattttaat tatatggatc gttttgcttt tttccctttt ttcttgtgct acatctactg
     1801 cgttgcggga atcccttgct ttcgaatatt ttactttagc tgaggaatat tacgaactta
     1861 aaaaatggga taaagcacta gagttttaca caaagagtag tcaaagtgaa aacgtatata
     1921 ctaattcttt gtataagata ggccttattt ttgttgaaca aaaaaaatat gataaagctg
     1981 aaaatgtatt tgacgaccta ttaaaaaaag atacaaaaaa tgcaacaata ctttctcaac
     2041 tcgcttttgt taaatcaaaa caaaaaaaat tcctagaggc aatttattta tacgaggaca
     2101 ttcttttaat gtatcccatg gatcaaacaa cgaagaaaaa cctcgcgctt gtgtattggt
     2161 ataatggcga tacggaaaaa gctcgtagta tacagaaaga gctcatagat gaaaatcccc
     2221 tggatacaag cttgaaagat ttattgaaag aagatgaagg caataaagct ggctaaagat
     2281 aaaactacag ttgtttttct atagtgtatt atagacgtaa tctttatatt tatctggatt
     2341 tgctttgaat gctataatcg ggtcttcttt ttcggttaaa gctttgtcaa ttgcctgttg
     2401 tgcttctttc aaaattcttg ttcctgggat aatacgtaaa gagcgtgtcg aaataccaat
     2461 ggcaattttt acatcgcaat tttttagtat ttcatctaag gcaatataga gttcttcggc
     2521 aagtgtaagc gctttttcta catttaaatc ttggtagatg caagcaaatc catcttgacc
     2581 gtattcaaaa ataaggtctc gaaacttaaa agcttcgagc aaaacctctg aaataaactc
     2641 tgtatacgcg ggataatgct ctataccagc tatcttgatg aggacaaggg cgaggtcttg
     2701 ttcggatgag ctggctcgta taagttcaga gtcgagtcgc ggttctaggt aggattccca
     2761 gccaaaaccc gttttttctg aaaataatcc gagggggtcg gatattcttt taccttggat
     2821 ttgaaatttt gcatattgtt ttttattttc tctgtctaaa aagctatctt tttttacttg
     2881 gtctttcact tgttcttcca aaggaacagt agcaatcgaa tccttttttt ctatatctaa
     2941 agcaaattct gaatcgctta taggatcatt atgtaaaaaa gcatcagtat ctgttttgta
     3001 atcaaaatca aagtctaaat caaagtcatt tttttgaaaa tcattgtaaa aattggaatt
     3061 ctgagaatct tgaaatgcaa tttcgcttgt aggaaaacta tcggactctg tgcgattatt
     3121 tttctgtaaa attttgttta taataattgt taaaaaaaca aaaagagtgg taagaaaaag
     3181 tataaaaaag cttttttttg ctaggtcatg aatgagataa ggactgacgt gatacagggc
     3241 aaatgtaaga gtgataggct cattattatg aagcaaggaa gaactatata ccgaaataag
     3301 tggagattgt gttttaagta tgtttttact gttatcatct tgtgataaaa aagatgtatt
     3361 cttcggataa gaaaaaaatc cattatgttc tgtttctacg agtacaaggg ctatattagg
     3421 aaacatgtca agaacggcga taaagccttg cataaaatta tcatttatgc cgtcaaattc
     3481 ttcaatgaaa tcgaaggctt tttgtgttaa atcagtacat tgcacaataa tgttttctct
     3541 accttgctgt gaataggaaa tcattttaaa agtaaaaaag gcaatagcaa atagatacat
     3601 agtaattgca aaaaaagcaa taaagatagt tgtacttcga ttcatacgca ttattataat
     3661 tgcctatgag aagatttgca agcttctttt ctaaaaaaaa gcattattgt agatataaaa
     3721 gtgacttagt caaaaatgaa actgtatttt ctttgagttc tagaaaggat gttttcgttt
     3781 gttttttttg aaaggcgaga aattctttca gtgagatctc tctgtaaagc tcgtttattt
     3841 cggcagaaaa aggttgcgct tcaaactcaa gtgtagataa aagctttatg gtgttttcaa
     3901 gatagctttc taatcctttt gtatctattg tttgaaatat tttcttgcct tcgggggaag
     3961 gactttcttg tctttctaaa agaatctgaa cagcctcatt ctctgatata tcttttatag
     4021 ccggaaagga gttagaaaaa tccttatttt ctgctttaat tctataaatt ttatttttgc
     4081 ttgcgtgaat atttttaaac cagttttcat ataaggcgag gcttccgttt ccattgtatg
     4141 ctgctttcgt gattctatcc tctttaggtt taattcgaga atcacgagga gcattatagt
     4201 tttcaagagc atttggctga gaatgaggat agttttttgt atgggcgagg tcgagaccta
     4261 gaaaaaaaat atctttttcg ctggaatttt gtgaaaaacc taaggcggtg ccgcttaccg
     4321 tgccgcatcg taaggcagct tgtgaagaga gcaaggtttt ttttataacc tgatgagaaa
     4381 gtccgtcgcc ataatcaata aaatgaagga agttcttgtg aagaatgtta tagggaaggg
     4441 cagcttctaa agaagcaatg aagatggtgt ttttaaattg ctccccatat ttttctaggt
     4501 gcatttttgc ccaaaagcca ccgtcagttg taaaacagac attgggttct atcttgtact
     4561 tagagagtac aggcatggaa gatgaaagcg ctattaaaaa cattctgtct ttatgctttt
     4621 gtaaaaaatc tatcgttttt gttaaagaag gaccggatgc aatgatacag actggttttt
     4681 ctattctaaa agctggtatt attttttttg cgtattttat gtttcgaaat gtatttgtat
     4741 tccatttttt tgtaaaaaac aagcgtgttc ctaaaacatc acgtgaagta ttaaaaaaaa
     4801 tacgtaaaat ttccaaggtt ttttggcatt cttcgggaaa aagtctttca ctcgttggcc
     4861 aaattataac ttcggtaaaa agacttcctt cttcacctat gattttaaaa agttcatttc
     4921 caagcttgga agatgtccct gtgaaataaa aatcccaagg aatctcccga aaggctttgt
     4981 gatcaaaaaa ttctatagca ataaaacgag catttggaaa gcgttttttc aaaaaggagt
     5041 agctatgccc tccaaaaggt gcaataaaaa taatatagcg aggttctcca gaaaacgaaa
     5101 gcgtttttac aaagcgctct gcttctatga tgggattata acttgaatga ataaattgcg
     5161 tattccataa acagattttc tgattgtttt ttgatgtcgt aaaacgtaga ttcaccttag
     5221 gcttatctcg cgttgaattg atttgaatcg atgaaatttg ttgaattaaa tacattgttt
     5281 gggaaaaatg catcgatttt atctaggtca tttaatgaaa aatatttagt tatctcaaaa
     5341 gcacttactg ttgaagagtg taggtagtct gaaaagaaat tttttagaag tatcaaaagc
     5401 atttctatat caatgttctg attggaaaat acaacaagga tacatgagtt ttgtgagaga
     5461 aaggaaaaac ctggtttagg tagcactgta ttgcataaaa tcagaacaag ttcttttatt
     5521 ttttctaagc cagattctga aatattttca tttttttcta ggattttgtt ttctaactga
     5581 ataaaatggg caaatgaata aatagctaaa ccttgttgaa atacattttt ggatataatc
     5641 ggtttatttg gtttaagggc atctattgag ataaaaagtc tatcgataag ttgtataaag
     5701 tcaaaaatat ttctgtcatt tatcaataat gtattatgtt gtcttgattt attgttagaa
     5761 gaaaaaaaat aaatatagga attatcttcc gtattatatt ctttaaagta taaagaaaga
     5821 ttttcattta gttctgtagg aaaaaattga taaaaggcgg caaagtcatt acatgaaaaa
     5881 gaattccatg tatttttttt tgctagagtg ccatcccaaa aatccttaga agaatcgaat
     5941 agcttcattt ttgtgagatt aaaggcgtat ataattttat attgtttttc ttcctttttt
     6001 aaaattcctg aaaaaacgaa ttcatgtgtt tctgaaaaat ccttaaagaa gacatggctt
     6061 tcatcgagta aaagtgagtt tttgggaaaa ataggtttag attttgcaaa agcaagtttt
     6121 tcagcctttg aaatgagacc gcctgtcttt ttctgagacg gcttttttgg ttgagcttct
     6181 actttttgca ataaacccat ttacacaagt cctaattctg taaataattt tttataaacc
     6241 acaaagtttt cagattttgc aaattcttca attttatcct ctggaagatt ttctaaaagt
     6301 tggtccatat aaactaatac agattttata tcttcttgca tttgtttatc ttggatattt
     6361 tttgttattg aatttgtcgc aggtggctct gttgctggta ttgatttggc ttcattctct
     6421 aaacttggac ttgcatcatc atcaaaaccc cattgttcgc tgttaaatac ctcatcggtg
     6481 ggctcgttgt aagaattttc atcaatttcc gtgtctcggt caggttcaat agcttcagga
     6541 attatctcag atagatcttc ttcttcttct tcttcttctt cttcttcttc ttcttcttct
     6601 tcttcttcga catcaagcag taattcatcc tcaaagtctg ctatggcgtt tatttcagga
     6661 ttttcaagat cattgtcttg gtccacagga acttctagta aatcgctgtc agctgaaggt
     6721 acaacgagct cagagctgtc gatagtgata gtatcaatta attcttcgtt gttagccaca
     6781 attgtgttta tgtctaattc ttcttcactt ggttctatta agttttcttc aaaaaattct
     6841 tctacaagct catcgtcgtc gagggaaatc tcggcatcgt c
//
LOCUS       DAOSSW010000084         6730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_10491_length_6730_cov_7.901423, whole genome shotgun sequence.
ACCESSION   DAOSSW010000084 DAOSSW010000000
VERSION     DAOSSW010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10491_length_6730_cov_7.901423"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..454
                     /locus_tag="PKW26_07070"
     CDS             116..454
                     /locus_tag="PKW26_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOQ93363.1"
                     /translation="MAQKIYVGNMNYATTEDALTSLFSQYGEVVSAVVIKDRYTDQSK
                     GFGFVEMTDAEAADAAIATLNGKDLDGRKLRVNIAEEKPRNSRPRGNFGGNHGGGYNR
                     DRQGGYNSGY"
     gene            525..1391
                     /locus_tag="PKW26_07075"
     CDS             525..1391
                     /locus_tag="PKW26_07075"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOQ93364.1"
                     /translation="MDTFKDNARWAFLKAFTEKDCDRFSFIKEFLERVAIPFSVLHID
                     DKKHFYIQFNKTSYTPMFNIKTVIAHYDRDENSPGANDNSASVFQLIDWARQLSTSID
                     LHNVRIILTDGEELGSAKEQGAYGIASVFNRLGIKNDSVYVLDGCGRGEVLVVSSTGK
                     NAPGNSQFRKKFSSLYEESIALARAVAPESWITVPVPYSDNAGFIAAGIPAIALTVLP
                     KDEATAYMRTLQKERKFEKEVLAQRADKTKLPKTWNLMHSEGDTIESLTDESFDLMAS
                     FLDALAKHKSIA"
     gene            complement(1388..2242)
                     /locus_tag="PKW26_07080"
     CDS             complement(1388..2242)
                     /locus_tag="PKW26_07080"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOQ93365.1"
                     /translation="MIEAIKLFAIGILLGLANIIPGVSGGTMAIVFNVYENIIAIISF
                     NIKKILKEWKFILPLGLGMVSGIFLFSSFLSFLLARFPIQTAVFFIALICGSLPLIRS
                     KISKEAFVSTKLSSSLAFLIALGLMLLMFFISPNEAGQVYDQINPALFLRLTISLAVA
                     AIAMIIPGVSGSFLMMVLGIYATVIASISHMRIPLLIPIALGVILGLLSGAGLVRFLL
                     AKAKTQTYAAILALVIMSIPLLFPLNAIKAFLQSATQLSVLVLIVSSLLVFVLGFCLS
                     FFTSREKN"
     gene            complement(2274..3845)
                     /locus_tag="PKW26_07085"
     CDS             complement(2274..3845)
                     /locus_tag="PKW26_07085"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOQ93366.1"
                     /translation="MEFLNFDELEAYKKLLSLENAVALPSILSAERVKKYSIPMAEGL
                     VYNYAAKEVNAQVLSVLQDLSDEASLVEKFKLLYEGEVMNTGENRKVLHHLTRGQLGA
                     PVLHEGEDLFAFYTEQQRAIANFASDVHAGKITNEKGESFTTVIQIGIGGSDLGPRAM
                     YLALEQWARAAGKQKMKAHFISNVDPDDAAAVMASVDMSRSLFILVSKSGTTQETLAN
                     ELFVKDFLKKANLNPAKHMLAVTSKTSPLANNPDYLKSFYIDDFIGGRYSSTSAVGGA
                     VLSLALGAETFAQFLAGAHEADKTAKEKNIEKNPALMDALIGIYERNVQGYPATAVLP
                     YSQALSRFPAHLQQADMESNGKGVNRFAQPISYQTGPLLFGEPGTNGQHSFYQLLHQG
                     TAICPLQFIGFKESQAGKDIEVQNSTSQEKLNANLAAQIVAFARGTKNENPNKNFSGG
                     RPSSLILGKSLNPRSLGALLAHFENKIMFQGFVWNINSFDQEGVQLGKVLTQKVLSKD
                     MDEVLASYAALFDLA"
     gene            complement(4030..6438)
                     /locus_tag="PKW26_07090"
     CDS             complement(4030..6438)
                     /locus_tag="PKW26_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOQ93367.1"
                     /translation="MQRKTLEQLDYFRIQNAIAFYCKTEEGREALLERLPLSDEKEIR
                     TLKELGFEMKGYVAKGFSSLIRPFEAVAALFPKISVEGAALNLEEIHRLGEFLLSVQM
                     LHENKNALLEKNENLDTFKNLFPLLDSIPLLSSETEEVYRIIDRSGQMKDLPQLRAIR
                     KSIASLQSSIDKSLKAHLHNPLLKDALQAELPALRGDRQVLAVKANFRGRVRGIVHEL
                     SQTGQTLYIEPEDVVEKNNALVQEEHRLQVEIKKILQALCSLLRPSIPLFQAAHRNLL
                     FLDALNATALWATEHNCHFAFEKKLSLLQARHPLLGSDCVPIDIVFQKDCRVLIITGP
                     NTGGKTVSLKTIALFSLLNQAGFPIPAQEGSSLPYFSSVFADIGDEQSMDESLSTFSA
                     HMKNISAMLSASDEKSLVLLDELGSGTDPQEGGAIAMAVLDALIEKKAYVLVTTHHGV
                     LKNYGFTHPSCVNASVAFDSTSLSPTYKILMGVPGESHAINIARKSGLPESIVDQAER
                     YMAGGDADVSALIKALTEKLDGLSEKEKELEKKERLTHEAVKKADLKALRIRQQELQL
                     KDAQIKESNRFLSDSRKMLENLVREIREAELTKATTKKVKDSIAALEAGLEDEKRRQD
                     EKKEILAKDLDAQEKAADKTKSSKTKLEAKDFQKDDRVIINSTKMKGVIVSKDKKNSY
                     LVLVGSVKMSVSKSNLSLDTREVQLGSPSIQFDAAPSSGDKPVFELRLLGMRGEEALQ
                     ALEKQLELCILHAVSSFSIIHGKGDGILQNLVHEFLKNYPGIRSFDFAPPEDGGTGKT
                     YVTL"
     gene            complement(6438..>6730)
                     /locus_tag="PKW26_07095"
     CDS             complement(6438..>6730)
                     /locus_tag="PKW26_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOQ93368.1"
                     /translation="TKKTSSLAVQKNAVEVIDTPGIRRFVLSEVEDTELALYFREIAN
                     LVGTCKFGLSCKHETEPDCAIIQAVEEGRMSPERYENYLRIADEIKTDSWED"
BASE COUNT         1941 a         1383 c         1398 g         2008 t
ORIGIN      
        1 cttccagctt ttattttcgg gaaaatatca caaactaaga aaacgactct ttttaccatc
       61 ttagtgtggt aagtgatacg aaatgccgac aaaggctcgg caagaggatt ttttaatggc
      121 tcagaaaatt tatgttggaa acatgaatta tgcgacaaca gaagatgctt taacaagctt
      181 gttttcgcag tatggtgaag ttgtatcagc agtcgtaatt aaggaccgct atacagatca
      241 atcaaaagga tttggttttg tagaaatgac cgatgcagaa gcagcagatg cagctattgc
      301 tacattaaat ggcaaagact tagacgggag aaaactccgt gtaaatattg cagaagaaaa
      361 accacggaat tcaagaccac gaggtaactt tggtggaaat cacggcggtg gttacaacag
      421 agaccgacaa ggtggctata attctggcta ctagctaatg agagccctga aaagtaatgc
      481 tttttggggc ttttttttat cctttttctt ccgatactat ctatatggat actttcaaag
      541 acaatgctcg ttgggctttt ttaaaagcat ttacagaaaa agattgcgac cgcttttctt
      601 ttattaaaga atttttagaa cgtgtcgcca tacccttttc agtattgcat atcgacgata
      661 aaaaacattt ttatatccaa ttcaataaga catcttacac tcccatgttc aatattaaaa
      721 cggtgatagc gcactatgac cgcgacgaaa actctcccgg cgcaaatgac aattctgcaa
      781 gtgtctttca actcatcgat tgggcgagac aattatctac ctcgattgac ttacacaatg
      841 tgcgtattat tctcaccgat ggggaggagc tcggttcagc aaaagaacag ggtgcctatg
      901 gtatagcaag tgtttttaat cggctcggta taaaaaatga ttctgtctat gtgctcgatg
      961 gttgcggtcg aggagaggtt cttgttgttt cgagtaccgg aaaaaatgct cccggaaata
     1021 gtcagtttag aaaaaagttt tctagcttgt atgaggaaag tatcgctctg gcgcgcgctg
     1081 ttgccccaga atcttggatc actgtgcccg taccctatag cgacaatgca ggttttattg
     1141 cagcgggcat tcctgcaatt gccctaacgg ttttgcctaa agacgaggca accgcctaca
     1201 tgcggacttt acaaaaagag cgaaaatttg aaaaagaggt tttggcacaa agggcagata
     1261 agactaagct cccaaaaacc tggaatctca tgcatagcga aggcgatact atagaaagct
     1321 taactgatga aagctttgat ctcatggcgt cttttttaga tgccttggca aagcataaaa
     1381 gtattgccta gtttttttct cgagatgtaa aaaacgaaag acaaaatccc aaaacaaaga
     1441 ccaataaact cgaaacaatt aaaaccaaaa ccgatagctg tgtagcgctt tgtaaaaagg
     1501 cttttatggc attcagggga aaaagaaggg ggatagacat aatcacaagt gctaagattg
     1561 cggcataggt ttgtgttttg gcttttgcca ataaaaagcg tacaaggcct gcaccgctta
     1621 aaagtcctag aataactcca agggcgatgg ggataagaag aggtatacgc atatgagaaa
     1681 tcgaagcgat aactgttgca tatatgccta aaaccatcat taaaaaagag ccagaaacac
     1741 cggggattat cattgcaatg gctgctacgg caagcgagat tgtaagtctc aaaaaaagtg
     1801 cgggatttat ctggtcatag acttgtcctg cctcatttgg cgaaataaaa aacatgagaa
     1861 gcatgagccc aagggcgatt aaaaaagcaa ggcttgaaga aagctttgtc gatacaaagg
     1921 cttcttttga aatctttgaa cgaattaagg gaaggctccc gcagataagg gcaataaaaa
     1981 aaacagctgt ttgtatggga aagcgtgcga gaagaaagct taaaaaactg ctaaataaaa
     2041 aaataccaga taccattccc aaaccaagcg gcaaaataaa cttccattct tttaagattt
     2101 tttttatatt aaaagaaata atcgcgatta tattttcata gacgttaaag acaatcgcca
     2161 ttgtgccgcc agaaacacct ggtataatat ttgcaaggcc gagcagaatg cctattgcaa
     2221 aaagttttat tgcttcaatc atgagagcca ccttttagat tgcttttttt tatttatgca
     2281 agatcaaata aggctgcata tgatgctaaa acctcatcca tatcttttga caaaactttt
     2341 tgtgtcaaaa ctttgccgag ctgcacgcct tcttgatcaa agctgttaat attccacaca
     2401 aatccttgga acataatttt attttcaaaa tgtgcaagta aggcgccgag gcttcttggg
     2461 tttagacttt tcccaagaat aagacttgag ggacgtcctc ccgagaagtt tttatttggg
     2521 ttttcatttt ttgtgccacg ggcaaaggcg acaatctgtg cagcgaggtt ggcattgagt
     2581 ttttcttggc tcgtggagtt ttgcacttct atatccttgc ctgcttggct ttctttaaaa
     2641 ccaataaatt gaagggggca tatagctgta ccttgatgca ataattgata aaaggaatgt
     2701 tgcccatttg ttcccggctc tccaaaaagc aggggacctg tttgatacga aataggctga
     2761 gcaaaacgat ttaccccttt accattcgat tccatatcgg cttgttgcaa gtgcgcagga
     2821 aatcgtgaaa gcgcttgact atagggaagc actgcagttg caggatagcc ctgtacattt
     2881 ctctcgtaga ttccgatgag cgcatccatg agggcaggat ttttctctat gtttttttct
     2941 tttgcggttt tatcagcctc atgagctcca gctaaaaatt gcgcaaatgt ctcagcaccg
     3001 agtgctaaag aaagcaccgc tccacctacg gcagaagtag aagaatagcg ccctccaata
     3061 aaatcatcta tgtagaaaga tttaagataa tcgggattat ttgcaagggg acttgtcttt
     3121 gaagtaacgg caagcatgtg ttttgccgga ttgagatttg cttttttcaa aaaatctttt
     3181 acaaagagct catttgcgag ggtttcttgc gttgtaccag actttgaaac caagataaag
     3241 agactacggc tcatatcgac agaagccatc accgcagccg catcatcagg gtctacattc
     3301 gaaataaaat gagccttcat tttttgcttg ccggcagccc ttgcccattg ttcgagggcg
     3361 agatacatgg cccgcggccc gaggtctgaa ccaccgatgc cgatttgtat aacagtggta
     3421 aaggattcac ctttttcatt tgtaattttt cctgcatgta catcagaagc aaagtttgct
     3481 attgcccttt gttgttctgt ataaaaggca aaaagatctt cgccttcgtg aagaacagga
     3541 gcgccgagtt gtcctcgcgt aagatgatga agcacttttc gattttcgcc tgtattcatc
     3601 acttctccct catagagcag cttaaatttt tctacaaggg atgcttcatc actcaagtct
     3661 tgcaaaacag aaagcacttg tgcatttact tcctttgcag cgtagttgta gacaagcccc
     3721 tcagccatag ggatgctgta ttttttaaca cgctcagccg acaaaatcga tggcaatgct
     3781 accgcgtttt ctaaagacaa taatttttta taagcctcta attcatcaaa attcaaaaac
     3841 tccatataat aactccttat ctaatctcaa aattataagt ataaattttc ctaccagatc
     3901 gccatgaagg attaaagtgc tctttcacag tcaaatcttt gtgtaaatat tcaatataat
     3961 gaataagctt tccctttctg tcaagataga gactgctctt tttgtctttt tatacaaatg
     4021 aatacaaggc tagagtgtca cgtaggtttt tccagttcct ccgtcttcag gcggagcaaa
     4081 atcaaaacta cgaatgccag gataattttt caaaaactca tgaacgaggt tttgcaaaat
     4141 accgtcgccc tttccatgga taatagaaaa cgaagagacc gcatgcagga tacaaagctc
     4201 aagctgcttt tccaaagctt gtagggcttc ttctcctcgc atacccaaaa gacgcaattc
     4261 aaaaaccggc ttatctccag aactaggtgc cgcatcaaat tgtatggaag ggcttccaag
     4321 ttgcacttcc cttgtgtcga gagagagatt gctttttgaa actgacattt ttaccgagcc
     4381 caccaaaacg aggtagctgt tttttttatc ttttgagaca attacgccct tcatttttgt
     4441 agaattgata atcacccgat catctttttg aaaatctttt gcttcaagct ttgtttttga
     4501 gcttttggtt ttatcagccg ctttttcttg ggcatctaaa tcttttgcaa gtatttcttt
     4561 tttctcatcc tgcctgcgct tttcatcttc taagccagcc tccaaggcag ctatgctatc
     4621 ctttactttt tttgtcgtag cctttgtaag ctcagcttct cgaatttctc gcactaagtt
     4681 ttcaagcatc tttctgctat ctgataaaaa acggtttgac tcttttatct gtgcgtcttt
     4741 taattgcaat tcttgttgcc gaatgcgtag ggcttttaag tcagcttttt ttaccgcctc
     4801 atgtgtcagc cgttcttttt tttccagctc tttttctttt tcacttaagc catcgagttt
     4861 ttcagttaag gcttttataa gggcagaaac atccgcatct ccgccagcca tataacgttc
     4921 agcttgatcc acaatggatt caggtaagcc agacttccga gcaatattta tcgcgtgact
     4981 ttctcccggc actcccatga gtattttata cgtcggtgaa agagaagtgc tatcaaaggc
     5041 aacggaagcg ttgacgcagc tcggatgtgt aaagccataa tttttaagta cgccgtgatg
     5101 ggtcgttacg aggacatagg cttttttttc tatgagcgca tcgaggacgg ccatcgctat
     5161 ggcgccacct tcttgtgggt cggttccact gcccagctcg tcgagcagga cgagggattt
     5221 ttcatcagag gcagagagca ttgcagaaat atttttcatg tgcgcagaaa aagttgataa
     5281 ggactcatcc atcgattgct catcgccaat atctgcaaag acagaagaaa aatacggcag
     5341 gctcgaacct tcttgtgcag gaataggaaa gcctgcttga ttcaaaagcg aaaacaaggc
     5401 aattgttttt aagctaactg tttttcctcc cgtatttggc cccgtaataa tgagtacccg
     5461 acaatctttt tgaaagacaa tatcgatggg aacgcagtcg gacccaagta agggatgccg
     5521 tgcttgaaga aggctgagtt ttttttcaaa ggcaaagtga cagttatgct cggttgccca
     5581 aagcgctgtt gcattcagcg catcgaggaa aagaaggttt ctatgtgccg cttgaaaaag
     5641 tggaatagaa gggcgtagaa gagaacaaag cgcttgtaag atttttttta tttctacctg
     5701 tagtcgatgc tcttcttgca caagggcatt gtttttttca acaacgtctt ctggctctat
     5761 gtagagcgtt tgccccgttt gcgaaagctc atgcacaatg ccacgcaccc tgccccgaaa
     5821 atttgcttta accgcaagca cctgtctgtc tccacgtagg gcaggaagtt cggcttgcaa
     5881 ggcatctttt aaaagaggat tgtgcaaatg cgcttttaaa cttttatcga tacttgattg
     5941 taaagaagcg atggattttc gtattgcgcg caattgcgga aggtccttca tttgcccaga
     6001 cctatcgata atgcgataga cttcttctgt ttcgctcgaa agcaggggaa tagaatcaag
     6061 caaggggaag aggtttttga aagtgtcaag gttttcgttt ttttctagaa gagcattttt
     6121 gttttcgtga agcatttgca cagagagcaa aaattctcca aggcgatgta tttcttctag
     6181 gtttaatgca gcaccttcaa cggaaatttt gggaaacaat gcagcgactg cttcaaaggg
     6241 acgaatgagt gaagaaaacc cctttgcgac atagcccttc atttcaaagc caagctcttt
     6301 taaggtgcga atttcttttt catcgctgag aggaagtcgt tcaagaagcg cttcccttcc
     6361 ttcttctgtt ttacaataaa aagcaatcgc gttttgaatt ctaaaataat ctaattgttc
     6421 aagggttttt ctttgcatta atcttcccaa ctatcggttt ttatttcgtc tgctattcgt
     6481 agataatttt cataacgctc aggactcata cgtccctctt ccaccgcctg aataatagca
     6541 cagtctggct ccgtttcatg tttgcaggat aagccaaatt tacatgtgcc gacaaggttt
     6601 gcaatctcac gaaaataaag cgcgagttct gtatcctcta cctcgcttaa aacaaagcgt
     6661 ctaattcctg gcgtgtcgat tacttctacc gcattttttt gaacagcaag tgagcttgtt
     6721 ttttttgtat
//
LOCUS       DAOSSW010000085         6694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_10552_length_6694_cov_6.790179, whole genome shotgun sequence.
ACCESSION   DAOSSW010000085 DAOSSW010000000
VERSION     DAOSSW010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10552_length_6694_cov_6.790179"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PKW26_07100"
     CDS             <1..855
                     /locus_tag="PKW26_07100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOQ93369.1"
                     /translation="DPLGAKKYHVTERLIHQYKNRVLLLSTGLCFAYCRFCFRKNFTG
                     KEAGWISEDEIEKACSYIAGHPEVKEILISGGDPLLASNERLAFLLEAIRSASPNILI
                     RLGTRSLFFAPERFTESTIELLQSFAPLWIIPHINHPLEISEDYTPEVLSAIAKIRSA
                     GIPMQSQTVLLKGVNDSLSILERLFHNLVCLGIKPGYLFQLDLAQGTSHFRLSIERAL
                     ALYADLKKELSGLSCPIFAVDLPGGGGKLNLEGLMQKEIGICYTKTESSILVENKKKD
                     ESWSYPRN"
     gene            complement(879..5450)
                     /locus_tag="PKW26_07105"
     CDS             complement(879..5450)
                     /locus_tag="PKW26_07105"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOQ93370.1"
                     /translation="MKRLHLLFLVSILFSIFSLGLFSQDFYWEAPVSISRSHAYFPSF
                     AKSSATGNDSIIIWQEVENNAIWISTAVYRFTGQGYEWVKNERFAGPFPFSGEIPQIS
                     TVAMNKRGQIACAIIADTHTISVYSSSDSGQSFAKTNIEHRGEPLLAPRIYATSKNGF
                     MIFASEGDDEAFLLRQAESADGYTWSAFREFEPSKHITNPFIPVLTETKEGDLVVFQA
                     SHSSGTRLSYQLFSSLSTDGGRNWSPVKLLTDETQRVLGEENQIFTNFHNQKANLLYF
                     NGKTYIAWERTHYASENAAIYFSELQNANTLGPIEKISSGQGNASNPILFAYNNTISL
                     VWFDTRSGKEDIYFTQKTGLIWSDENRLSSGTNSALFGIPIVSDNGKSVDFFWQEQNT
                     RSSHIVHLAPDRTIAKPSIIARNFIVGKKAASERVQFQIQLPQDSSGIAGFSYLWTKN
                     PYLEAPRILQNLPSQTALTEWANEDGDWFLKVKALDYAGNWSEDASISYTRDTKPPEK
                     PIIHLPDFDDDGFVISNTLPFTWEASDDETIAYSYSLEYMDAVQKLPITGEQELKREP
                     AKPPLRILTENTKLNLTNIENGFYAFSVSAIDEVGNVSEPTIITIAFNKFIPYTAVTN
                     LSSERTLFGNYVLSILGRGFSSEGFVSKIGLLNESTNEERFFYYDAGDYKIVSDRSIR
                     ELDIGDMEEGSYKVRLYHEKRGLYSIGQKLTILETGTVKIGDYSYVYAPQIRGLNEGR
                     AFSIGIHELLISLLIVLVFIILLVSIQGIISSSKEMLLIKQEVQILITGVSMTKPSRK
                     KTRELYHKGMSLKYKLVFFTSSLIIMIIILVSLPLSYLMTSTQEETLAEGLEEKTEVL
                     LESLASSVKAYLPTQNVLELSFLPNQSNALKEALYTTIVGLPAAGDNTDISFIWATND
                     TEIETYIDTKEINFGASRYTRDYIETISEKAHLANDLAVEKVSHLADEITRLTAEGIG
                     LAGRNDADSIERLNEIQNATRSLNESLSEELNQLSKEYTESIPAFNTKNLSRDTTDYI
                     FYKPVLYRQGTEKDFVRGIVILSVSTESLIYSVESAQKTILITTGIIALLALSIGLII
                     TLILASVIIKPIRQLAAHVAMIRDTDDKEQLEGKDILLKSRDEIGLLGDTVNDMAQGL
                     VRAAAASKDLTVGKEVQKMFIPLETDSFGKKLTTGSEVNENAEFFGYYEGAKGVSGDY
                     FDYKKLDDTWYAIIKCDVAGKGVPAALIMVEVATLFLNYFKDWSFKTHGTKLDTIVSQ
                     INDLIESRGFKGRFAAFTLCLLNSISGEAYFCNAGDNLVHIYSKRQKKNIIETLPDSA
                     AAGVFPSFMVDLKGGFQTVKLNLQKGDVLFMYTDGIEEAKRNFRDSSFKIIECAEAGL
                     NIDDPHENHSVGQDNEELGPDRVNAIVEAVFDRKKYRLTKFHNPIVNEVLEFDFSSLS
                     GTAEDAIMALVSVEKIFRMYKDPSATDFDRVQVDRKIDSFLEKVFVQYNSYCANKIDH
                     PSLDEYLLYKGIKEDEQYDDLTLVAIKKK"
     gene            complement(5447..>6694)
                     /locus_tag="PKW26_07110"
     CDS             complement(5447..>6694)
                     /locus_tag="PKW26_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93371.1"
                     /translation="LENLKRQTQSRILIAEQEILLAERAKNEALLRVSQVQEAIRNNA
                     FQNARDNLARARTKYNESLEHQESESLRKESDEQLILLANEITRKENEVVIRDVRNLK
                     NEAKTAYYQGNFERAETLLIQAENRFAVTNVGEKDPEITNLLILVGTALSMKTGRVIL
                     PSAPLYPEMSQIMSLAKQYFEKGKRLLAENKRAEALTVLNDAKKKIRELQIVYPLNQE
                     ASLLTLRIDQLIDPAAFESFFAQRITQAKQDFRDLTKRQSAYTDLLDLSEINPRYPGL
                     SDFIYNAEIEMGIRIRPPDQKALAESRRLTNEAALVVNSASRDEIQLNAALAKLNTAL
                     ENNPDNEEAMVLKDRVQIMIGGKASIVLSSESEDLYQRAILELQRGNVLQAAGIVNTL
                     LQKRENQNSSKIIDLKKKVDSLL"
BASE COUNT         1822 a         1353 c         1299 g         2220 t
ORIGIN      
        1 gatccacttg gtgcaaaaaa ataccatgtt actgagcgcc tcatacatca atataaaaat
       61 agagtactgc tcttgagtac cgggctttgt tttgcctatt gtcgtttttg ttttcgaaaa
      121 aattttactg gaaaagaagc aggttggatt tctgaagatg aaatagaaaa agcttgtagc
      181 tatatcgcag ggcatcctga ggtaaaggaa attcttatct caggcggaga cccgcttctt
      241 gcctcaaatg aacgtcttgc atttttacta gaggctattc gcagcgcaag tccaaacatt
      301 ctcatacgcc ttggaactcg gagtcttttt tttgcaccag agcgttttac tgaaagcaca
      361 atcgagcttt tacagtcttt cgcccctcta tggattattc ctcatatcaa tcatccctta
      421 gagatttctg aagactatac gccagaagtg ctatccgcaa ttgccaaaat tcgatctgca
      481 ggtattccca tgcaaagtca aactgttttg ctcaaagggg taaatgattc cctctctatt
      541 ttagaaagac tttttcataa tctcgtctgt cttggaataa aaccgggcta tctttttcaa
      601 cttgacttag cgcaggggac gagtcatttt cgactttcca tagaaagagc ccttgcccta
      661 tatgcggatc ttaaaaaaga gctctcaggt ctgtcttgtc ctatttttgc ggtagacctt
      721 cctggtgggg gagggaagct caatcttgaa ggcttgatgc aaaaagaaat cggtatctgt
      781 tataccaaaa cagaatcgag cattcttgta gagaacaaaa agaaagacga aagctggtct
      841 tatccgagaa attaaaatgg cacctttctt ttacaagctt attttttctt tatagcgaca
      901 agggtaaggt cgtcgtattg ctcatcttct tttataccct tgtacagtaa atactcatcg
      961 agtgatgggt ggtctatctt gttagcgcag tagctattgt actgaacaaa gaccttttcc
     1021 aaaaagctgt caatttttcg atcgacttgg actcggtcaa aatcggtagc gcttgggtcc
     1081 ttgtacattc taaaaatttt ctcaacactc acgagggcca taattgcgtc ttcagctgtg
     1141 ccagatagag agctaaaatc aaattccaaa acttcattta cgatgggatt gtgaaatttt
     1201 gtaaggcgat atttttttct gtcaaatacc gcttcgacga ttgcatttac gcgatctgga
     1261 ccgagttctt cattatcctg tccgacagag tggttttcat gtggatcatc tatgtttaaa
     1321 ccagcttcag cacattcaat aattttaaaa ctgctatctc taaagtttcg ttttgcttct
     1381 tcaataccgt ccgtgtacat aaacagaaca tcgccttttt gaagattgag ttttacggtt
     1441 tgaaaaccgc ctttgaggtc taccataaaa gaagggaata caccggccgc tgcagaatcg
     1501 ggcaatgttt cgataatgtt ttttttctgt ctcttcgaat atatatgcac gagattatcc
     1561 cctgcattgc aaaaatatgc ttctcctgaa attgagttga gcaggcagag cgtaaaggcg
     1621 gcaaaacgtc ccttgaaacc acgcgattca atgagatcat ttatctggga gacaatagta
     1681 tcgagctttg tgccatgtgt tttgaaagac caatctttaa aatagtttag gaaaagcgtt
     1741 gcaacttcaa ccatgataag cgctgcagga acacctttcc ctgcaacgtc gcattttata
     1801 attgcatacc aggtatcatc aagtttttta taatcaaaat agtcccctga aacacccttt
     1861 gcgccttcgt agtagccaaa aaattcagcg ttttcgttta cttcacttcc cgtggttagt
     1921 ttttttccaa agctatctgt ttcgagtggg ataaacattt tttgcacttc tttacccacg
     1981 gttaagtctt tagaagcagc ggcagcgcga acaagtccct gcgccatatc atttaccgta
     2041 tcgccgagca agcctatttc atcgcggctt ttgagaagaa tatcttttcc ctcaagttgt
     2101 tctttatcat cagtatctcg aatcatagca acgtgggcag cgagttgccg tatcggttta
     2161 atgataactg aagcaagaat gagtgtgata attaagccta tggatagtgc aagtaaggca
     2221 ataatacctg tcgtaataag tattgttttt tgtgcacttt caacggaata aattaaagat
     2281 tcagtactta cggataagat gacgattccg cgaacaaagt ctttttctgt tccctgtcta
     2341 tataaaacag gtttgtaaaa aatgtaatcg gttgtatctc ttgaaagatt ttttgtatta
     2401 aatgcaggta tagattctgt atattctttt gaaagttgat ttagctcttc gcttaaactt
     2461 tcattgaggc tccgtgttgc gttttgaatt tcatttagcc gctctattga gtctgcatca
     2521 tttctgcccg caagacctat cccttctgcg gtaagccgag ttatttcgtc agcgagatgg
     2581 cttacttttt ctacagccaa gtcatttgca agatgagcct tttctgaaat tgtttctata
     2641 tagtctctgg tatatcgtga agcgccaaaa tttatttctt ttgtatcgat atatgtttct
     2701 atttctgtat catttgttgc ccaaataaaa gagatatcgg tgttgtcgcc agcagcagga
     2761 agcccaacga ttgttgtgta caaggcttct ttcagggcat ttgattggtt tggtaaaaaa
     2821 ctgagttcca aaacattttg cgtgggtaaa tacgctttta cagaggaggc aagagattcc
     2881 aacaatacct ctgttttttc ttcaagtcct tctgcgaggg tttcttcttg ggtgcttgtc
     2941 ataagatagc tgagagggag agagacaaga ataataatca tgataattaa gctcgaggta
     3001 aaaaagacga gcttgtattt tagactcatt cccttgtgat agagttccct agtttttttc
     3061 cgagaaggct ttgtcatact aactcctgta ataagtatct gtacttcttg ctttatcaat
     3121 agcatttctt ttgagctgct tataattcct tgtatcgata caagcagtat aataaaaacg
     3181 aggacaatta aaaggcttat gagcaattca tgtattccaa ttgaaaaagc tcttccttca
     3241 ttgagaccgc gaatctgagg agcatataca tatgaataat ccccaatctt cacagtacct
     3301 gtttcaagga ttgtaagttt ttgccctatg ctgtagagcc ctcgtttttc atggtagagg
     3361 cgtactttgt atgaaccctc ttccatgtcg ccgatatcga gctcacggat acttctgtct
     3421 gatacaatct tatagtcccc cgcatcatag taaaaaaatc gctcttcgtt tgtgctttcg
     3481 tttagaagac ctatctttga aacaaagccc tcagagctaa agccccgccc tagaatcgat
     3541 aagacatagt ttccaaagag cgttcgctcg gagctaagat ttgttacagc ggtgtagggg
     3601 atgaatttat taaaagcaat agtaataata gtcggttcac tgacatttcc aacctcatcg
     3661 atagctgaga cagaaaaggc ataaaaacca ttttcgatat ttgtcagatt tagttttgta
     3721 ttttctgtga gtatacgtaa gggcggtttt gctggctctc tttttagctc ttgctctcca
     3781 gttattggta gcttttgtac agcatccata tactcaagag agtaggagta agcaatcgtt
     3841 tcgtcatcgc ttgcttccca ggtaaaggga agagtattcg atataacaaa gccatcatca
     3901 tcaaagtcgg gtagatgtat gattggtttt tcaggcggtt ttgtatccct tgtatagcta
     3961 atgctcgcat cttctgacca gtttcctgca taatcgaggg ccttcacctt tagaaaccaa
     4021 tctccatctt cgtttgccca ttctgtgaga gctgtttgac ttggaaggtt ttgtaaaatc
     4081 ctcggtgctt ccagataggg atttttcgtc catagataac taaagcctgc tatacccgaa
     4141 gagtcctgtg gtagttgaat ttgaaattgt actctttcac tggcggcttt tttccctaca
     4201 ataaagttac gggctattat agacggctta gcgatcgttc tgtcaggtgc aagatgaacg
     4261 atatgtgagg accttgtatt ttgctcttgc caaaaaaaat ctacactctt gccattatca
     4321 cttacaattg ggataccaaa cagggcacta tttgtcccgc tagagagcct attttcatcg
     4381 gaccatatga gaccagtttt ctgtgtaaaa taaatatctt cttttccact tcttgtatcg
     4441 aaccaaacaa gggaaatcgt attgttgtag gcaaagagaa ttggattgga agcattgcct
     4501 tgaccactcg atattttttc aataggcccc aaagtatttg cattttgcaa ttctgaaaaa
     4561 taaatagcag cattttccga ggcataatgc gttctttccc aggcaatata ggtttttccg
     4621 ttgaagtata gtagatttgc tttttgattg tggaaatttg taaaaatttg attttcttcg
     4681 cccaagacgc gttgggtttc atctgttaaa agctttactg gcgaccagtt tctaccacca
     4741 tcagtgctca agctggaaaa taattgatag gaaagccttg taccactgct atgggaagct
     4801 tgaaatacaa caaggtcgcc ttcttttgtt tctgtaagca caggaataaa aggatttgtg
     4861 atatgtttac ttggctcaaa ttctctaaaa gcactccagg tatagccatc tgctgattct
     4921 gcttgtcgca gtaaaaaagc ttcgtcatca ccttcggaag caaagatcat aaaaccgttt
     4981 ttacttgtgg catatattcg tggcgctaag aggggctcgc ctctatgctc aatatttgtt
     5041 tttgcaaaag attgcccact atcgctactg ctatacacag atatagtatg agtatctgct
     5101 ataatcgcac aggcaatttg tcctctttta ttcattgcaa ccgtactaat ctgtggaatt
     5161 tctccagaaa agggaaaggg accggcaaaa cgctcgtttt ttacccattc atagccttgt
     5221 cctgtgaaac gatagaccgc ggtagaaatc caaatcgcat tgttttctac ttcttgccat
     5281 attataatag aatcattccc cgtggcgctt gattttgcaa agctggggaa ataggcatga
     5341 gagcgagaaa tggaaacagg cgcttcccaa taaaaatctt gtgaaaacaa gccaaggcta
     5401 aaaatcgaga aaagtatact gactaaaaat aacaaatgaa gccttttcac agaagcgaat
     5461 ccaccttttt ctttaaatca ataatttttg aagagttttg gttttccctt ttttgcaata
     5521 aggtatttac aatacctgca gcttgtaaaa catttcctct ttgcaattct aaaatagctc
     5581 tttgatataa atcctctgac tcagaagaaa gcacaatgga agcttttcca ccaatcataa
     5641 tttgtaccct atctttcagc accatagctt cttcattgtc tggattattt tccaatgcgg
     5701 tatttaactt cgcaagtgca gcattgagtt gtatttcatc cctacttgca gagtttacca
     5761 caagagcagc ctcatttgta agccgtcttg attccgcaag cgctttttga tctggtggac
     5821 gtatacgaat ccccatttct atttccgcat tgtagataaa atcactcaaa cctggatatc
     5881 gtggatttat ttcagaaagg tcgagtaaat cagtatatgc gctttgccgt tttgttaaat
     5941 ctctaaaatc ttgttttgcc tgtgtaatac gttgtgcaaa aaaactctcg aaggcagctg
     6001 gatctatgag ttggtctatg cgcaatgtca gcaagcttgc ttcctggttc aatggatata
     6061 caatttgcaa ttcacgaatt tttttctttg catcattgag tactgttaag gcttctgcac
     6121 gcttattttc tgccaataat ctttttccct tttcaaaata ttgttttgca aggctcatta
     6181 tttggctcat ttctggatag agcggcgccg aaggtaaaat aacgcgtcct gttttcatcg
     6241 aaagcgctgt tccgacaagt atgagcaagt ttgttatttc tgggtctttt tccccaacat
     6301 tggtcaccgc aaacctattt tcagcctgta ttaaaagcgt ttctgctctt tcaaaatttc
     6361 cttgataata ggctgttttt gcttcatttt tgagatttcg cacatctcga atgacaactt
     6421 cgttttcctt tcgcgttatt tcattcgcta aaaggatcag ttgctcatcg ctttcttttc
     6481 gtagactttc agattcttgg tgttctaagg attcattgta ttttgttctt gcacgggcta
     6541 aattatccct ggcgttttgg aaggcattgt ttcgtattgc ttcttgcact tgactaactc
     6601 gtaaaagcgc ttcgtttttt gcacgctctg ccaataatat ctcttgctct gcaatgagaa
     6661 tacggctttg cgtttgtctt tttagatttt ccaa
//
LOCUS       DAOSSW010000086         6691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_10554_length_6691_cov_5.279385, whole genome shotgun sequence.
ACCESSION   DAOSSW010000086 DAOSSW010000000
VERSION     DAOSSW010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10554_length_6691_cov_5.279385"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PKW26_07115"
     CDS             <1..263
                     /locus_tag="PKW26_07115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOQ93372.1"
                     /translation="KLIAKLILASCPVKGIVLDPFLGSGTTSVVAKKLGRNYIGIEMN
                     EEYCLWAEKRLEQAETEKTIQGYTDGVFWERNTLQFQNKKQK"
     gene            377..2227
                     /locus_tag="PKW26_07120"
     CDS             377..2227
                     /locus_tag="PKW26_07120"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOQ93373.1"
                     /translation="MKKIALKVFLASLASSCIVAIIIVSGMLYSFSSINEYSTTTIHD
                     TLLQDYDNLIESEIDTAYSILEFYYAQYKNNLMTNTEAQKAAADLIRELRYGAEGYYW
                     IDTSKGDNIVLLGSATEGTNRMDLQDATGKYIMKAIIGAALDGTGFSDYLFPKEGGEE
                     ALPKRSFSKHFEPWDWVIGTGNYIDDINIVVNEKKAIIQSMVQRALYFMLVLFTIGLL
                     TTAIISYLFGRTIAKPIVSVSAILKDLAEGEADLRNRLPVLTKDEVGQLSGNFNNFLG
                     KLASIIQSINESMRKTIEIKERMTNGTHETLSALHEITANTNSMNSQLQNLDGQIDTV
                     DKASASIEKNIQSVDTGIESQASAVEQTTASVNQMVASLKNVSNITEKKAQTTKDLVS
                     VFETGKDNMNDTRSIISRINDSIGSINEMINMINSVASQTNLLAMNAAIEAAHAGEAG
                     RGFAVVAAEIRKLAETSSKNAKDIATNINNILSLINEADTSSDKTLKTIEHISQEIIE
                     TSHSFEEILSNTHELFIGGEQILSALTSLTQISVQVKDNSRVVKEDSLRMNKSISRVT
                     TISDEVVSGMNEIAVGSHEITESMASIDQQMQTLSEITNVLQKEVAKFKV"
     gene            2338..3642
                     /locus_tag="PKW26_07125"
     CDS             2338..3642
                     /locus_tag="PKW26_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOQ93374.1"
                     /translation="MKENAILGPHDRPSLRRWIPLSFQHVFAMFGATVLVPLLTGFSP
                     STALFTAGTGTLIYILCTGAKVPAFLGSSFAFIPALIGISQAYGQAYALGGAICAGLF
                     YAIVAGSIKVSGSGWIDKVLPPVVIGSVIIVIGLNLAPTAMNSAMFDQSGSYSLVYLS
                     IAFVTLSIAIIASIFFKGFFNTISILIGLIGGYLFTLIMGFIFPSYAIIDFSIVENAK
                     WFGLPFLQLNEMGKYYWLAPKFALVPILTFIIVSLATICEHLGDILVTSKVVGQDFYK
                     KPGLHRTLLGDGLATAWAGIWGGPPNTTYGENIGVMAITKVYSVWVIGGAAVIALLLS
                     FIQKFGAIIQTIPGPVMGGISMLLYGLIASSGLRTIVESGVDYKDKRNLTISSVILVI
                     GIGGGLLEFHVGSNFTFSLGGVALATVIGIILNAIIPKTTIE"
     gene            complement(3720..6188)
                     /locus_tag="PKW26_07130"
     CDS             complement(3720..6188)
                     /locus_tag="PKW26_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOQ93375.1"
                     /translation="MKFGHFDDSKREYVIETPETPYPWINYLGNEKFFSLISNTAGGY
                     SFYTDARLRRLTRYRYNDVPLDNNGRYFYINDNGTVWNPGWKPMRVNLDSYECRHGFG
                     YSKILSTKNALRAEVLYLVPNGDNVEIQMLRLRNESDEKKTIALHSFVEWCLYNAHDD
                     NTNFQRNFSIGEVEVLDSTIYHKTEYRERRNHYAFYHVNAPIDGFETDRETFLGLHNN
                     YDKPNAVFASESSNFVADGWSPVASHRINLELNPGEEKTYVFLLGYIENEEEQKWASK
                     TNEKLLQTNAQMRQINKDKAIALQKKYDSVEKVQKAFDELEVFWSQIIDTFTLNTDDV
                     KLNRMAVWNQYQCVVTYNFARSASYFESGIGRGIGFRDTSQDMLGVVHSLSQSRVRER
                     LFDVASTQFEDGSAYHQFQPLTKRGNADIGGNFNDDPLWLILGVGAYIKETGDTAFLK
                     EMVPFDNDENNKASLFEHLRRSFHHPLNNSGPHGLPLIGRADWNDCLNLNCFSTDPND
                     SFQTSTNKDGTNAESVMIAQMFVYVAPDYAAMCRLMGNEEEAVFAEEAAKKMADRICD
                     VGWDGEWFVRAYDDFGNKVGSKECEDGKIFIETQGFGSMAQIGADKGYPIKALDSVAK
                     HLDTDYGIVILDPPYKDYHVELGEVSSYPPGYKENAGIFCHNNPWIMIGEVQSGRSDK
                     AWAYYKKIAPAYLEDISEVHRLEPYVYAQMIAGKAARRHGEAKNSWLTGTAAWNFVAL
                     SQWICGIRPDYDGLIIEPRLPAHIKKAEITREYRGCIYKISIENKNPKGTIELGSTDS
                     VVLDGTKVLAKEGVREVAIKVCVG"
     gene            6340..>6691
                     /locus_tag="PKW26_07135"
     CDS             6340..>6691
                     /locus_tag="PKW26_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HOQ93376.1"
                     /translation="MSSNSIHDTARQNNEIAVSSMIILASYFLAQILADIGSLKIASI
                     GKMSFDAGTLIYPFTFTLRDLIHKKLGKKTAQRMIVLCAGANLIMAGFFALVTLLPSD
                     PTWTLQAAFAAVLGP"
BASE COUNT         2051 a         1415 c         1228 g         1997 t
ORIGIN      
        1 aaaaactcat tgccaaactc attcttgcaa gttgtcccgt caaaggtatt gttcttgatc
       61 cttttttggg ttcaggcacg acttccgttg ttgcaaaaaa actgggcaga aactatatag
      121 gcatcgaaat gaatgaagaa tattgtcttt gggcagaaaa acgtcttgaa caagcagaaa
      181 ccgaaaaaac aattcagggc tacaccgatg gggtattttg ggagcgaaat accttgcagt
      241 ttcaaaataa aaaacaaaaa taaacagcta atatatctac tcataaaaac ctttttttat
      301 cttttttgta ttgaaaattg aataaaaacc gattaaaata tactatacta tttatgtttc
      361 caggtaagga gtatctatga aaaaaattgc acttaaggtt tttttagcat cactagcaag
      421 tagttgtatc gttgcaatta ttattgtaag tggaatgtta tatagctttt catctattaa
      481 cgaatatagc accacgacaa ttcatgacac acttttacaa gactacgaca atctcattga
      541 atctgaaata gataccgcct attctatctt agaattttat tatgctcagt ataaaaacaa
      601 tctcatgacc aatactgaag cccaaaaagc tgcggcagac cttattcgag agcttcgtta
      661 tggggcagaa ggctactatt ggatcgatac ttcaaagggg gataatatcg tactgctcgg
      721 tagtgcaacc gaaggaacaa atcgtatgga tctccaagat gcaacgggaa agtatattat
      781 gaaagcaata ataggagcag cgctcgatgg aacaggtttt agcgactact tattcccaaa
      841 agaaggtgga gaagaagcgc tccctaaacg aagtttttca aaacattttg agccatggga
      901 ttgggttatt ggaacaggta actatataga tgatataaat atcgtcgtaa atgaaaaaaa
      961 agcaattata caatcaatgg tccaaagagc tttgtatttt atgttagtat tgtttacaat
     1021 aggtttactt acaacagcta ttatttccta tcttttcggt agaactattg caaagccaat
     1081 cgttagcgta agcgctatcc ttaaagacct tgcagagggt gaagccgatt tacgcaaccg
     1141 cttacctgta ctcacaaaag atgaagttgg gcaattgagc ggtaatttta acaatttctt
     1201 aggaaaactt gcctcaatca ttcaatctat aaatgaatca atgagaaaaa caatcgaaat
     1261 aaaagagcga atgacaaatg gtacacatga aacactctct gctttgcatg aaatcaccgc
     1321 caatacaaat tctatgaact cacagcttca aaacttagat ggtcaaatcg atactgttga
     1381 caaagcctct gcttccatag aaaaaaacat acaatctgtt gatacaggca tcgaatcaca
     1441 agcaagtgcg gtagagcaaa ccactgcttc agtaaatcaa atggttgctt ctcttaaaaa
     1501 cgtctcgaac ataactgaga aaaaagcgca aacaacaaaa gatcttgttt ctgtgtttga
     1561 aacaggaaaa gacaatatga acgatacgcg ctctataatt agcagaataa atgatagcat
     1621 cggaagcatc aatgaaatga taaatatgat aaactctgtt gcttcacaaa ccaacctgct
     1681 tgctatgaac gcagcaatcg aagcggctca tgcaggtgaa gctggacgag gttttgctgt
     1741 tgttgccgct gaaattcgaa aacttgcaga aacttcttca aaaaacgcaa aagacattgc
     1801 aacaaacatc aacaacattc tctctctgat aaatgaagca gatacttcaa gcgataaaac
     1861 cttaaaaaca atagaacata tcagccaaga aattattgaa acctcacatt cttttgaaga
     1921 aatcttatcg aatacccacg aactatttat cggtggagaa caaattcttt ctgcactgac
     1981 ttctttaaca caaatcagtg tacaagtaaa agataactca agagtcgtaa aagaagattc
     2041 tcttcgtatg aacaaatcga tatctcgtgt taccactatc tcagatgagg tcgtttctgg
     2101 aatgaatgaa atcgctgttg gttcacatga aataacagaa agcatggcga gcatcgacca
     2161 acaaatgcaa acactttctg agatcacaaa tgttctccaa aaagaagtgg caaaattcaa
     2221 agtctaaatt tttctttcat ctatttgcaa aaccaagaag ctcgtgttat tatgaggctt
     2281 atacaaaatt caggtttaga cctaggattt aaaaacgaat aagataggag caaaaaaatg
     2341 aaagagaacg ctattttggg gccacatgac aggccttctt taagacgctg gatacctttg
     2401 agttttcagc atgtgtttgc aatgtttgga gcaaccgttt tggttcctct tttaactgga
     2461 tttagccctt caacggcatt atttaccgcg ggaacaggaa ctttaattta catcctttgt
     2521 actggcgcaa aggtccccgc cttccttggt tcttcctttg cctttatccc tgccctcatc
     2581 ggcatatccc aagcatatgg ccaagcctat gcactcggtg gtgcgatttg tgcagggctt
     2641 ttttatgcca tcgtcgccgg ctctataaaa gtttctggtt ccggctggat agataaggtc
     2701 ctaccaccag tcgttatagg ctcagttatt attgttatag ggctcaatct tgcccccacg
     2761 gcaatgaatt ctgctatgtt cgaccaaagt ggcagctact ccttggtgta tttatctatc
     2821 gcctttgtaa cgctctctat cgcaattata gcaagcattt tctttaaagg cttttttaat
     2881 acaatttcta ttttaatcgg tctcattggc ggctatcttt tcacactcat tatgggattt
     2941 atttttccct cctatgctat catcgatttt tctatcgtag aaaacgcaaa gtggtttgga
     3001 ctgccattct tacagttaaa tgaaatggga aaatattatt ggcttgcacc aaaatttgcc
     3061 ttagtaccca tcctcacctt tatcattgtt tctttggcaa caatctgtga gcaccttggc
     3121 gatattctcg taacctcaaa ggttgttgga caagattttt acaaaaaacc tggactgcat
     3181 cgcacacttt taggtgatgg gcttgcaaca gcttgggctg gtatttgggg tggtcctcca
     3241 aacacaacct acggcgaaaa catcggcgtt atggctatca caaaggttta cagtgtttgg
     3301 gtaatcgggg gagctgctgt cattgccctc ttgctttcct tcattcaaaa atttggtgcg
     3361 ataatccaaa caattcccgg tcccgttatg ggcggcatct ccatgctcct ctacggactc
     3421 atcgcttcga gcggtttacg caccatcgta gaaagtggcg tcgactataa agacaaacga
     3481 aacctcacta tttcatctgt tattctcgtc atcggcattg gcggagggct tttagaattt
     3541 cacgtgggtt caaactttac cttctcactc ggcggcgttg cccttgcgac cgtcatcggg
     3601 atcattctca atgcaatcat ccccaaaaca actatagaat aacaaaaaac aataaaaaag
     3661 gctggtttta cccagccttt tttatacctg tcaataaatt cgaaatcaat taagatctat
     3721 tatccaacgc agaccttaat agctacctct cgcactcctt cttttgcaag cactttcgtt
     3781 ccatcgagaa ccactgaatc tgtagagccc agttctatcg tccccttggg atttttattt
     3841 tctatggata ttttataaat gcagccgcga tactctcgtg taatctctgc ttttttaata
     3901 tgagccggaa gtcttggctc tataatcaag ccatcataat ctggtcgaat accacaaatc
     3961 cactgcgaca aggcaacaaa attccatgct gcggttcctg taagccaact attctttgct
     4021 tctccgtggc gccgtgcagc cttacctgct atcatttgag cgtagacata gggttcaaga
     4081 cgatgaactt cactaatgtc ttctaaatag gcaggggcaa tttttttata ataggcccat
     4141 gccttatcgc ttctacccga ttgtacctcg cctatcatta tccatggatt attgtggcaa
     4201 aaaattcctg cattttcttt gtaaccaggc ggataggatg aaacttcccc aagctcaaca
     4261 tgatagtctt tatagggtgg atctaaaatc actatgccat agtcggtatc taaatgcttt
     4321 gcaacactat cgagagcctt aatgggatag cctttatccg caccaatttg tgccattgaa
     4381 ccaaaaccct gtgtctctat aaatattttt ccatcttcac attcttttga gccaaccttg
     4441 ttgccaaagt cgtcgtaggc acgaacaaac cattctccgt cccatcctac atcgcaaatt
     4501 ctgtctgcca ttttttttgc tgcttcttct gcaaacaccg cctcttcttc attgcccatt
     4561 aaacgacaca ttgcagcata atctggtgcg acatagacaa acatttgggc aatcatcacc
     4621 gattcagcat ttgtaccgtc tttatttgtc gatgtttgaa atgaatcgtt cgggtcggta
     4681 ctaaaacagt ttaagttaag gcaatcgttc caatcagcac gcccgataag tggtaaacca
     4741 tgaggaccgc tattgttgag aggatggtga aaggagcgac gaaggtgttc aaaaaggctt
     4801 gccttgttat tttcatcgtt atcgaaggga accatctcct tcaaaaaggc tgtatctcct
     4861 gtttccttga tataagcgcc aaccccgaga atgagccaca agggatcatc gttaaaattc
     4921 cccccaatgt cggcatttcc tcgctttgtc agtggttgga attggtggta ggcacttcca
     4981 tcttcaaact gtgtcgaagc tacgtcgaaa agcctttctc gaacacgcga ctgcgaaagc
     5041 gaatgcacaa caccgagcat gtcttgactc gtatcacgaa atccaattcc tcgcccaatg
     5101 ccactttcaa aatacgaggc agaccgtgca aaattgtagg taaccacaca ttgatattga
     5161 ttccacacag ccatgcggtt caattttaca tcgtctgtgt tgagagtaaa ggtgtcaatt
     5221 atctggctcc aaaaaacctc taactcatca aaggcttttt gtactttttc tacactatcg
     5281 tatttttttt gcagagcaat agccttatcc ttattgattt ggcgcatctg agcatttgtt
     5341 tgcaagagct tttcatttgt tttgcttgcc catttttgtt cttcttcatt ttcaatatag
     5401 ccaagtaaaa aaacataggt tttttcttca ccgggattaa gttcaagatt tattcgatga
     5461 cttgcaaccg gagaccaacc atcagccaca aagttcgagc tttcgctggc aaaaacagca
     5521 ttaggcttat cgtaattatt gtgtaaacct aaaaatgttt ctctatctgt ttcaaaaccg
     5581 tcgatcggag catttacatg ataaaaggca taatgattgc gcctttctct atactccgtt
     5641 ttgtgataaa tcgtactgtc tagaacttca acctctccaa tagaaaagtt tcgctgaaag
     5701 tttgtattat catcatgcgc attgtaaaga caccattcaa caaaagaatg aagcgctatg
     5761 gtcttttttt catcgctttc atttcgcaag cgaagcattt gtatttcgac attgtcgccg
     5821 tttggaacaa gatataaaac ctcggcccgc aaggcatttt ttgtagatag aatcttagag
     5881 taaccaaaac catggcggca ttcgtagcta tcaagattga cacgcatggg tttccaacca
     5941 ggattccaca cggtaccatt atcattgata taaaaataac ggccgttatt atctaagggt
     6001 acatcattgt atcgataccg agtaagtcga cgaagacggg catctgtata aaaagaataa
     6061 cctccagctg tgttagaaat gagagaaaag aacttttcat tccctaagta atttatccat
     6121 gggtaaggcg tttcaggagt ctctattacg tattcccttt tactatcatc aaaatgccca
     6181 aatttcattg cattctcctt ataaatcaac ttagttagtt gaaagactca actaaccttt
     6241 ataatagcat atttttatat ttagtcaagc gaaaaatgag aaaaatgcta tctagaccca
     6301 agctacaaaa aaaagtatta tgttaataaa ggataggtta tgtcgtctaa tagtatacac
     6361 gatacagcaa ggcaaaacaa tgaaatagca gtctcatcta tgataatttt ggcaagttat
     6421 tttcttgcac aaattttggc agatattgga agtttaaaaa ttgcatcaat tggcaaaatg
     6481 agtttcgatg cagggacgct tatctacccc tttaccttta ccttacgaga cctcattcac
     6541 aaaaaactag gaaaaaaaac agcacaaaga atgatagttc tttgtgcagg agcaaatctt
     6601 attatggcag gtttttttgc tctcgtaacg cttctaccct cagacccaac gtggaccttg
     6661 caagcagctt ttgcagcagt tttgggacca g
//
LOCUS       DAOSSW010000087         6501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_10939_length_6501_cov_5.537698, whole genome shotgun sequence.
ACCESSION   DAOSSW010000087 DAOSSW010000000
VERSION     DAOSSW010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10939_length_6501_cov_5.537698"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    105..204
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(430..6360)
                     /locus_tag="PKW26_07140"
     CDS             complement(430..6360)
                     /locus_tag="PKW26_07140"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOQ93377.1"
                     /translation="MNLLFFSFAGGKKDVEEVSVEDLKSWQETVDLENKKTGKHNVLI
                     TAEDMGGNVTHQGPFNIFVDPESDRPVTMIVNPRENMRVPANLNIVGSCVDDDAVGSV
                     HIVLDGDRENEIVAEGKDFWSYYLDTTQMEDGPHEIIVYGTDHKGIVGREQKVVWHLD
                     RKQPQIEVDSHEMGTLVSKKVKISGTVADGNGIESFYYSLDRGENYMPVSIKQDKKTK
                     LWHFSINIDTQKMDDGAAVFWFKAIDGQGSEGYLTFLYFIDNTAPDVSIIYPDEKEAV
                     NGLFSIAGYAKDDIGVESLTWTFGKETGEIALTPGNPYWTLDLDVRGEKLKSENFSIT
                     ARDTIGNVTTKKLKVLVDNEQDLARIDLASPSEKEDFGDALLLRGIAKDDDGIAEIFF
                     SIDSGEVYSFPSEGVFSVSASDFLDEPLSVGAHELQIWARDIHGVDGKIEKRLFYRSG
                     PLPGFSNASVITGSKADEKTEVYQSGMIVHPEAGSQFSIDIESLCGLAELSWSFDGME
                     EKTKSFVGGKNTTGARGVSNLRIPFDSAWGSSQLRIRAKDIFGRTSEWIASFYITNLT
                     KTQGAPELDFSDFLDSKDRILLGEEEVHGFFIGGKAAEVRFVPETDFASLRLSGNTIS
                     IIPSNEKGLSEKVQVEVTTDSGATYLSNAFVLYTKVPKPEITTEETVFDGLSTVSVAG
                     SISSSLLIESLRYRILYAKTPATEWQYLNLDEEGAFSFSCETELFENGASIIEIQAID
                     EIGTLSSKGLLVKKIPPVLDPKKANPFDKPNIYWFEGENIYFASVYTGTLSLSSFTIN
                     GNADASIDRPVFGQIDFENLSPGDNRFDLSIANEADKLTASRFTVKKDSSPRIHFVSA
                     LDMPIVSGLEVAISRKNPTKTESLLINIESPHAISAAQYAFFNEAPIKLSLKKSPTDA
                     NLYVGEIPLHTLSAEIVPITVSVNSLKGKTITKKRSISIVRPLHEQQIQDAKQIYWQA
                     KKEGEYPHYILEKGALLSAYVNALTPVKVLWEEPNDALVLEVKNKTVFVKAKENGLYE
                     NLVLIAIDAEGTEIRSNPISIVVDSELPSLFVNEPENKQWLTNSLYISGESEDKTALK
                     EVSYSLDGGKSWVPVSLDEEEKLEKNRSTFSKTIDISHLADGLIQLDLRAVDVAGKES
                     QKSFALHKDTEAPSVSVVLPYSQDVVNGETTIVFDVRDEGKVAKAEYVLFERGEEEMI
                     EVARNALDLDRFVISQIGTKEQPITNAMVFEFTDYAGNVRHLNSWDFFVEVISDLPVV
                     EVHIPVENEIIRSDFVVSGVVYDDDGPCRIWYAIDDEEFTLLDTEYANGFSIDIPLSR
                     MTDNEHIVSVYAEDLYGVLGEKIVRPFRVSLEEPKGSVDTPYLDKTVRDRVEISGTAS
                     DKNGIKKVEISVDNGISFADVVGTENWSYEFDTKILEDGSHVVFIKVWDNYDIEALYS
                     SILNVDNTKPQIQLEYPEDNSTTTGKVLFSGQTTDNIGLETLYIRVKPLDSSMPAVPV
                     EFSRFDFVPGEIINSTIDLSGLSDGFYNVELSGEDAAGNVSRVSRNIQLTKDTTLASI
                     DILYPLTGETVQGMFNLYGQVSSEYPMQTVILFIDEQNIASVDVTDSGYFMFTLTPED
                     LLEGSHKLQVRGIVEGTKIISSVDHYIHYTPNGPWVTIDNFIMGDFAYDRPYLEGSSG
                     YAFTEEEMLALKDKTVSKEEKARIQAKAVDFIELSFDNGKTFKRISAKKKWRYRVENE
                     DMAQGSYYLLVRAVMENGESAVTKTIVQIDKTPPIIKLISPEEGGRFNEQLEIAGLTS
                     DDIALKSVDIAFRKGDKSNYEVPSFIQGLYLDWHFWGATLFDIGVGLTFFDDNVRLQA
                     QFGQFTPTQWKLFVDNPFRYGGNVFGGKILANVGTVPFRYFLGPNWAWLSANFTIGAN
                     FSVFSLSQSGKAQVLSALLGQVEFPRVTIPKQSMFGTFAFYTEAQLWFIPTDVESGDT
                     EIESLVPQISVGIRMNVF"
BASE COUNT         1874 a         1443 c         1099 g         1985 t
ORIGIN      
        1 gcactatgct atgaaataaa atgtgatttt ttctcgtttt cataaaatcc tcctataaaa
       61 agtcaataag aaaatttcag ttttcgaaat tgactttttt cagcnnnnnn nnnnnnnnnn
      121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnncaaatg aacagtgaga cagcattttg tcgaactgtt
      241 cattaatctc ctataaaaag tcaataagaa aatttcagtt ttcgaaattg acttttttca
      301 gcactttcgc aatcttaatg agaaagtgcg gtaaatcaca cgtttgcttg caaacgtgca
      361 aatgaacagt gagacagcat tttgtcgaac tgttcattaa tctcctataa aaaacactca
      421 tccattgtct taaaagacat tcatacgtat gcccacagat atttgtggaa ccaaggattc
      481 aatttctgta tcgccagatt ctacgtcagt tggtataaac cagagttgcg cctcggtata
      541 aaacgcaaag gtaccaaaca tcgactgttt tggtattgtt actcgtggga attccacctg
      601 tccaagtaag gcagataaaa cttgtgcttt tccactttgt gataaactaa agactgagaa
      661 gtttgcacct atggtgaagt ttgcagaaag ccatgcccag tttggaccaa ggaagtatct
      721 aaagggcaca gtaccgacgt ttgcaagtat ttttccacca aagacatttc caccgtagcg
      781 gaaaggattg tctacaaaga gcttccattg cgtaggtgta aactggccga actgtgcttg
      841 taaacgaacg ttgtcatcaa agaaggtaag tcccacacct atgtcaaaaa gtgtcgctcc
      901 ccaaaaatgc cagtctagat acagcccctg tataaacgat ggcacttcat agtttgattt
      961 atcgcccttt ctaaaggcaa tatctactga cttgagggct atatcatctg aagttaaacc
     1021 ggctatttct agctgctcgt taaaacgccc gccctcttct ggcgaaataa gtttaataat
     1081 cggtggagtt ttatctattt gcacgattgt tttggttaca gcagactcac cgttttccat
     1141 caccgcccgt acaagtaaat aatacgagcc ctgtgccata tcttcatttt ctacgcgata
     1201 gcgccatttt ttcttcgcac taatccgctt aaatgttttt ccattgtcaa agctcaactc
     1261 tataaagtca actgcctttg cttgaatcct tgccttttct tcctttgaaa ctgttttatc
     1321 ttttaaggca agcatttctt cttcggtaaa ggcataaccg gagcttcctt ccagataggg
     1381 tcggtcgtag gcaaaatccc ccataataaa attgtcaatg gtaacccagg gtccattggg
     1441 ggtataatgt atataatgat cgacagagct gattatcttt gtcccctcta ctataccacg
     1501 aacttgtagc ttatgtgaac cctctaaaag gtcttccggt gtaagcgtaa acataaagta
     1561 accagaatcc gttacatcta ccgatgcgat gttttgttca tctataaaga gaataaccgt
     1621 ttgcatagga tactcagagc tcacttgccc atagaggtta aacattccct gcaccgtttc
     1681 acccgtcaag ggatacagaa tgtctatcga agcaagggtt gtatcttttg tcaactgaat
     1741 atttcttgaa actcgcgaca cgtttcctgc agcatcttca ccagaaagct ctacgttata
     1801 aaaaccgtcg cttaaaccgc ttaaatctat agtgctattt ataatttccc ccggcacaaa
     1861 atcaaaacga gaaaactcca ctggcaccgc cggcatactt gaatcgagcg gttttactcg
     1921 tatatagagt gtttccaagc ctatgttatc cgttgtttgt ccagaaaaaa gcacctttcc
     1981 tgttgtagta gaattatcct caggatattc aagctgtatc tgaggttttg tattgtccac
     2041 attgagaata gatgaataca atgcctcaat gtcataatta tcccatactt taataaacac
     2101 aacatggctt ccatcttcga gtatctttgt gtcaaattca taggaccagt tttccgtacc
     2161 aacgacgtca gcaaaactaa tgccgttgtc aacagagatt tcaacttttt ttatgccgtt
     2221 tttatcactc gctgttccgc ttatttctac gcggtctcgc actgttttat ctaggtaagg
     2281 tgtatcgacg ctgccctttg gttcctcaag cgaaactcta aagggtcgca caattttttc
     2341 acctagaacg ccatacaaat cttctgcgta aacgctaaca atgtgttcgt tatccgtcat
     2401 tctcgaaagc ggaatatcta tagaaaaacc atttgcatat tctgtgtcga gcagggtaaa
     2461 ttcctcatca tcgatggcat accaaatacg gcatggtcca tcatcatcgt aaacaacccc
     2521 agaaacaaca aagtctgagc gaatgatttc attttccacg ggaatatgca cttcaactac
     2581 gggaaggtca gatataactt caacaaagaa atcccaacta tttaagtggc gcacatttcc
     2641 tgcatagtca gtaaattcaa aaaccattgc attggttata ggttgctctt ttgtccctat
     2701 ttgactaata acaaacctgt cgaggtccaa ggcgtttcgc gccacctcaa tcatttcttc
     2761 ttcgcctcgt tcaaacaaga catattctgc ctttgctacc tttccttcat cgcgcacatc
     2821 aaatacaatt gttgtttcac cgttaaccac atcctgcgag tacggaagca caacagaaac
     2881 agaaggtgcc tctgtgtctt tatgtagagc aaaagatttc tggctttctt ttcctgccac
     2941 atctacggca cgaagatcaa gctgtatcaa accgtcagct aagtggctta tatctattgt
     3001 ttttgaaaaa gtgcttctat ttttttcaag cttttcctct tcatcgagtg aaacgggcac
     3061 ccaagacttt ccaccatcaa ggctgtagga tacttctttg agcgctgttt tatcctcact
     3121 ttctcctgaa atatacaatg aattggtaag ccattgttta ttttctggtt cattgacaaa
     3181 aagacttgga agctctgagt cgacaacaat cgatatagga ttcgatcgaa tctctgttcc
     3241 ctctgcatct atcgctatca aaacaaggtt ttcatacaaa ccgttttctt ttgcttttac
     3301 aaaaaccgtt ttatttttta cttctaaaac aagagcatcg tttggctctt cccataagac
     3361 cttaacagga gtaagagcgt ttacataggc actaagtaag gcaccttttt cgaggatata
     3421 atgcggatat tctccctctt tttttgcctg ccagtatatt tgttttgcat cttgtatttg
     3481 ctgttcatgc agaggacgaa caatcgaaat acttcttttt ttcgtaattg tttttccctt
     3541 tagactattt acagaaacag taataggtac tatttccgca gaaagcgtat gcaagggaat
     3601 ttctcctaca tagagatttg catcagtagg cgatttttta agagagagtt ttatcggcgc
     3661 ttcattgaaa aaagcatact gcgcagcact tattgcatgc ggagattcaa tgttaataag
     3721 taagctttct gtctttgttg gatttttacg cgagattgcg acctcaagtc cagaaacaat
     3781 cggcatatcc aaggcagata caaaatgaat acgaggactt gaatcttttt ttaccgtaaa
     3841 gcgcgatgcc gttagcttgt cggcctcatt tgcgatgctt aaatcaaagc gattgtcgcc
     3901 aggggagaga ttttcaaaat ctatttgccc aaaaactggc ctatctatgc tcgcatcagc
     3961 atttccattt atagtaaagc tggacaaaga aagtgtccct gtatataccg aggcaaaata
     4021 aatattttca ccctcaaacc aataaatatt tggtttgtca aaaggatttg ctttttttgg
     4081 atctagcaca ggcggtattt tttttaccaa aagccccttt gacgaaagag taccgatttc
     4141 atcaatagct tgtatttcaa taatcgatgc accattttca aaaagctcgg tctcgcagct
     4201 aaaggaaaaa gctccttcct catcgaggtt tagatactgc cattccgtcg ccggagtttt
     4261 tgcatacaag attctataac gcaagctttc tataagaaga gaagaggaaa tacttcctgc
     4321 aacactaaca gtacttaagc cgtcaaaaac agtttcttcg gtagttattt ctggttttgg
     4381 aaccttcgta tataaaacaa aggcatttga aagatatgtt gccccggaat ctgtagttac
     4441 ttctacttgc actttttcag acaaaccttt ttcattgctg ggaataatcg aaatggtatt
     4501 tccagaaagg cgcaaagaag caaaatctgt ttcaggaaca aagcgcactt ccgcagcctt
     4561 tccccctatg aaaaagccat gtacctcttc ctctcccaaa agaatgcggt cttttgaatc
     4621 caaaaagtct gaaaaatcaa gctcgggagc tccctgtgtt tttgtcaagt ttgtaatata
     4681 aaaacttgct atccattcgc ttgttcttcc aaaaatatct tttgctcgaa tgcgcaattg
     4741 tgagctaccc caagccgaat caaaaggaat acggagattt gaaactcctc gagcacctgt
     4801 tgtgtttttt ccgccgacaa agctttttgt tttctcttcc atgccgtcaa aagaccagct
     4861 caattctgca agaccacaaa gcgattctat atcgatagaa aactgactac ctgcctcagg
     4921 atggactatc ataccacttt ggtagacttc cgttttttca tcagccttgc ttccagttat
     4981 cacagaagca tttgaaaagc ctggaagcgg tccacttcta taaaaaagtc gcttttctat
     5041 ctttccatca actccgtgaa tatcccttgc ccaaatctgc aattcatgag cgccaacgct
     5101 caatggttca tccaaaaagt cagatgcaga gacagaaaaa acaccctcac tggggaagga
     5161 atacacttca cctgaatcta tcgaaaagaa aatttcagct atgccatcgt catcttttgc
     5221 aatgccacga agtaaaagtg catcaccaaa atcttctttt tcacttggag atgcaaggtc
     5281 tattcgcgct aaatcttgtt cattgtcaac caaaactttc agtttttttg tagttacatt
     5341 tcctatggta tctcgtgcgg taatactaaa gttttcagat ttcagctttt ctccacgtac
     5401 atcgaggtcc aaggtccagt agggattccc cggagtcaga gctatctcac ccgtctcttt
     5461 tccaaaagtc cacgtaagag actcaacccc tatatcatct tttgcatagc ctgctataga
     5521 aaacaagcca tttaccgctt ctttttcatc aggataaata atactcacat ctggtgcagt
     5581 gttatcaata aaatagagga aggttaaata accttcagaa ccctgcccat ctattgcttt
     5641 aaaccaaaaa acagcagccc catcatccat tttttgtgta tctatattga tagaaaagtg
     5701 ccataatttt gtttttttat cttgctttat agaaaccggc atataatttt caccacgatc
     5761 taaggaataa taaaaacttt caatgccatt tccatcagct accgttccag aaatctttac
     5821 tttttttgaa acgagggtac ccatttcatg actgtccacc tctatctgtg gctgttttct
     5881 gtctagatgc cagacgactt tttgctctct cccgacaata cccttgtggt cagtgccata
     5941 gacaattatc tcatgaggcc catcttccat ctgcgttgtg tcaagataat atgaccaaaa
     6001 atcctttccc tctgcaacaa tttcattttc acgatcgcca tcaaggacaa tatgaacgga
     6061 accgactgca tcatcgtcaa cacaggaccc aacaatattt aaatttgctg gaacacgcat
     6121 attttcacgc gggtttacta tcattgtaac agggcgatcc gattcgggat caacaaaaat
     6181 attgaaagga ccctgatgcg ttacattccc gcccatatct tctgcagtta taagcacatt
     6241 atgcttaccc gtttttttat tttctaaatc aactgtctct tgccagcttt ttaagtcctc
     6301 tactgaaact tcttcaacat cctttttgcc ccctgcaaaa gaaaaaaaga gaagattcat
     6361 actaataaaa ataaatacac attttttaaa caccagaaaa ccccttatca tgagatagca
     6421 ttccacttca attatcggca gaaatgtctc atttgtaaag cgttttttct actaaaacat
     6481 agaaaaatag agatttttta t
//
LOCUS       DAOSSW010000088         6372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_11228_length_6372_cov_6.594744, whole genome shotgun sequence.
ACCESSION   DAOSSW010000088 DAOSSW010000000
VERSION     DAOSSW010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11228_length_6372_cov_6.594744"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PKW26_07145"
     CDS             <1..663
                     /locus_tag="PKW26_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ93378.1"
                     /translation="ALYVSRIIMLRVPGANTYRTPNFPETALLTNSFLQKITNGSRLN
                     LGIIFVVLSVFIYWFIIEKTRTGFELRATGFNKDAARASGIPVVKSIMLSMAIAGAFA
                     GLAGGIVSLGAYKYGRVLAANDGYGFTGIAVALVGNNTAFGTALSGLLFGMLSTAQPL
                     MESNRIPKEITFIIQGLVVVFIAIRVGFRMLVAYFTKKAPLKQEKTLNIKNPQEEMPN
                     HE"
     gene            656..1561
                     /locus_tag="PKW26_07150"
     CDS             656..1561
                     /locus_tag="PKW26_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ93379.1"
                     /translation="MNSLFGKIPTILMLVSPILITATGGMICERSGVVNIALEGLMMF
                     GAFATATAHYFLEASFGPASIWLSLLIGGLFGLTFSLIHAFASISLKADQTVSGTGIN
                     LLATGVTIFLAQVFFNQDRTKQFMLGMMPNKAGIYPTAFIALVILILTWFILYKLPFG
                     LRLRACGENPHAAQSVGINVFRMRYIAVLTSGFLAGVSGGCLVLTTNTQFSSTTVNGH
                     GFIALAVVSFGRWKPLGIGGASLLFGTALAFSIIANDYTLLRKLPSEVFNIMPYAITL
                     LALVLFSGKNYAPLSAGKPYEKGAS"
     gene            complement(1653..2321)
                     /locus_tag="PKW26_07155"
     CDS             complement(1653..2321)
                     /locus_tag="PKW26_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93380.1"
                     /translation="MKILFACLLGLLAVIPSVLFQILIPPFYFSNPFLVLLQAIFITA
                     LIEEVSKALAFYLFPLRFLKTEKTKASRLKIFLGKKNLEKEVLKEEPICLDSDAILGE
                     DKKNACKEAKLVFLYGFFLGLSFGFYELLVYFISGEGALGLRFFTTVFFHAFCAVLGS
                     FFIFSWKKKKFWLFPIAFAILMHGLYNFFAQFTGTLWWFSILVLILTALQTFYAYSTI
                     SKSQ"
     gene            2380..2979
                     /locus_tag="PKW26_07160"
     CDS             2380..2979
                     /locus_tag="PKW26_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOQ93381.1"
                     /translation="MKIIVLCGIKHSGKSTLGKKVAKEINALFFDIDTVIEELESMTV
                     RELYKTQGKDAFMQAETRATKAILAELQSKNKGQTALISTGGGICDNEAALEALQNTK
                     PLMIFLDVPKEIAFSRIKDNALKTQSMPAYISDKNPQNEDEEKTIFYSYYEKRKEKYL
                     SFCKKEIQVNTEKNYALSTQKNVEKILSLIAESAFLEYP"
     gene            2983..4686
                     /gene="dnaX"
                     /locus_tag="PKW26_07165"
     CDS             2983..4686
                     /gene="dnaX"
                     /locus_tag="PKW26_07165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOQ93382.1"
                     /translation="MAYEVTATKRRPKRFEDLVGQDFVSFTLKNAISSGKIAHSYLFS
                     GPRGCGKTSAARILAKALNCETGPTASPCEICTNCLEISKGSSIDVIEIDGASNTSVN
                     DVRQIKDEILFPPNASTYKIYIIDEVHMLSISAFNALLKTIEEPPHYVIFIFATTELH
                     KVPATIKSRCQQFQFKLGSVEQIKNLLADTAEAAGIKAEEEALYWIAKESTGSFRDAY
                     TLFDQVASFSEGEISFEKIKNTLGLVGLDQTNSLFESCAEGKTETALSELDTILQSGI
                     TVDQTAVNCTEYLRSLLLIKSGIRKEALLGYAPQRYSQICIDFWTREQIEKALALFLQ
                     LYKDLRYSINPRYELELAVSRLCYLSSYTSPIEVKKALDQMRKILAQTSIEGNIEGKQ
                     AEGENPSFQKPIQQKQEVVIPETKNLEKEAPMNSIEQSLAEKEPPKKAANFAELKELV
                     LNELRINHSFLAPCIIQADWKNEEGVILFEVENSYAKTTIEKNRPIITKIIQDFWQEN
                     TKILVQLVEKITEQANDEKHPESVEAVCNMFKGKVISKTETAILENTTNNENGDDDES
                     F"
     gene            4655..4984
                     /locus_tag="PKW26_07170"
     CDS             4655..4984
                     /locus_tag="PKW26_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOQ93383.1"
                     /translation="MKMEMMMNPFEILKNAGAIKQQLEKTQEELDSVVETGSAGGAMV
                     KVTINGKFEMLKVEIDPLTVDPKETQMLQDLIVAAYNQAASKIQESLKDKVSSLTGGM
                     NIPGFNF"
     gene            4984..5571
                     /gene="recR"
                     /locus_tag="PKW26_07175"
     CDS             4984..5571
                     /gene="recR"
                     /locus_tag="PKW26_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOQ93384.1"
                     /translation="MNALDELTQSFSRLPGIGKKTASRISHSLLKMDQSFLTLFAHQL
                     LSLHERIKICSICGSYTEQDPCSICTDTMRDQALLCLVEDPRDVQTIEASHEFNGLFH
                     VLGGLISPLDGIGPDQLRIIPLINRIKENKITEVIIATNPTVEGDTTALYIQQILKPF
                     PVSVSRLASGLPVGGDLEYADKLTLARSFKGRTSL"
     gene            complement(5664..>6372)
                     /locus_tag="PKW26_07180"
     CDS             complement(5664..>6372)
                     /locus_tag="PKW26_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024752187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOQ93385.1"
                     /translation="MRSQIRGLNQASTNASNAISFIQATEGYLQNTTDIVQRLRELAV
                     QSANGIYSSEDRMQIQVEVSQLVAEVDRIANSAQFNGMNMLTGRFAKETGENVVTASM
                     WFHVGANMDQRIEAYIGTMTATALNLRNAGDESIITLEDPENANRAIGTLDEALKQIN
                     KQRADLGAYQNRLEHTIKGLDIASENLQAAESKIRDTDMASEIVELTKNQVLTQSGTA
                     MLAQANQSTQSVLSLLQ"
BASE COUNT         2088 a         1414 c         1125 g         1745 t
ORIGIN      
        1 gccttatatg tctcccgcat tattatgtta cgagttcccg gcgcaaacac ctaccgaacg
       61 ccaaactttc ctgaaaccgc ccttctcaca aattcttttt tacaaaagat aacaaatggt
      121 tctcggctaa accttggcat tattttcgtt gtgctttctg tttttatcta ttggtttata
      181 atagaaaaaa cacgcacagg atttgaactg cgcgcaacag gctttaataa agacgcagcc
      241 cgtgcctctg gcatacctgt tgtaaaaagc attatgcttt ccatggctat tgctggagca
      301 ttcgcaggcc ttgcaggagg cattgtctct ctaggcgcct ataaatacgg ccgagtcctt
      361 gctgcaaatg acggctacgg atttaccgga attgctgtcg ccctcgttgg gaataacact
      421 gcttttggca cagcactatc gggcctttta tttggaatgc tttccacagc acaaccgctt
      481 atggaatcaa atagaatccc taaagaaatt acctttatca ttcaagggct cgttgttgtt
      541 tttatcgcaa tccgtgttgg tttccgcatg cttgtcgcct acttcacaaa aaaagcccct
      601 ttaaaacagg aaaaaacact aaacataaaa aacccacaag aggagatgcc taatcatgaa
      661 tagtttattt ggaaaaatcc cgacaatact gatgctcgta tctcccattc ttattacagc
      721 aacaggcggt atgatttgtg agcgatctgg tgtagtaaac atcgcacttg aaggcctaat
      781 gatgtttggc gcctttgcaa ccgcaaccgc acattatttt ttagaggcaa gttttggacc
      841 agcttctatt tggctttctc ttctcatagg aggcttattt gggcttactt tctcactcat
      901 ccatgccttt gcttcaattt ctttgaaagc cgatcaaacc gtttcaggaa cagggatcaa
      961 cctacttgca accggcgtta ctatcttttt ggcgcaagtc ttttttaatc aagacagaac
     1021 caagcaattt atgctcggca tgatgcccaa caaggctgga atttatccca ccgcctttat
     1081 agcgctcgtt atactcatcc tcacatggtt tattttatat aaactaccct ttggacttag
     1141 gctgagagct tgcggcgaaa atcctcatgc cgcacaatcc gtcggaataa acgtttttag
     1201 aatgcgctat atcgctgtac ttacatcagg ttttttagct ggagtctctg gtggctgcct
     1261 cgtcctcaca acaaatacac aattcagctc aaccacagtc aacggacacg gctttatcgc
     1321 cctcgcagtt gtctcttttg gtagatggaa accactcggc atcggtggag cctccctcct
     1381 ttttggaaca gccctcgctt tttcaattat tgcaaatgat tatacacttt tacgaaaact
     1441 cccctcagaa gtttttaaca ttatgcccta tgcaataacc ttgcttgccc tcgtgctctt
     1501 tagcggtaaa aactacgccc cgctttctgc aggaaagccc tatgaaaaag gagcaagcta
     1561 aaataaaaaa gtccaaactc ctagcttgaa acaccttata tacaaaaaaa accgttttag
     1621 aaatcctaaa acggtttttt gtttttacat attcattgag atttagaaat tgtgctatac
     1681 gcataaaagg tttgcaaggc ggttaaaatc aagacaagaa tactaaacca ccacaaagtt
     1741 cccgtaaact gcgcaaaaaa attatacaaa ccatgcataa gtatagcaaa agcaatagga
     1801 aaaagccaga attttttttt cttccaagaa aaaataaaaa atgatcccaa aacagcacaa
     1861 aaagcatgaa aaaaaaccgt tgtaaaaaaa cgcaaaccca aggctccttc tccagaaata
     1921 aaatacacaa gcagctcata aaaaccaaaa ctgagaccta aaaaaaaacc atagagaaaa
     1981 accagcttcg cctccttaca agcgtttttt ttatcttccc ctaaaattgc atccgaatcc
     2041 agacaaatag gttcttcctt gagaacttct ttttcaagat ttttctttcc taaaaatatt
     2101 ttcaagcgag aggctttcgt tttttcagtc tttaaaaatc gcaatggaaa aagataaaaa
     2161 gcaagagctt ttgagacttc ttcaatcaaa gcagttataa aaatagcctg caacaaaacc
     2221 aaaaaaggat ttgaaaaata aaacggtgga ataaggattt gaaacaaaac actcggaata
     2281 acagcaagaa gcccaagcaa gcaggcaaaa agaattttca tacactaatc ataacccaat
     2341 caatatacaa tttcaagaaa ctttgataaa atcgtatcta tgaaaataat cgtcctctgc
     2401 ggcataaaac atagtggcaa gtctacgctt ggaaaaaaag ttgcaaaaga aatcaatgca
     2461 cttttttttg acatcgatac tgtaatagaa gagcttgagt ccatgacggt acgagagctc
     2521 tacaaaaccc aaggaaagga tgcttttatg caagccgaaa caagggcaac aaaggcgatt
     2581 ttagccgagc ttcagtcaaa aaacaaagga caaacagctc ttatatctac tggcggaggc
     2641 atttgcgaca atgaagctgc cttagaagcc ttgcaaaaca caaaacccct catgattttt
     2701 ctcgatgttc caaaagaaat cgccttttca cgcatcaaag acaatgcgct caaaacacaa
     2761 tccatgcctg cctatatttc tgacaaaaac ccacaaaacg aagatgaaga aaaaactatt
     2821 ttttattcgt attatgaaaa aagaaaagaa aaatacctca gcttttgtaa aaaagaaata
     2881 caagtcaata ctgaaaaaaa ttacgcccta tcgacacaaa aaaatgtcga gaagatttta
     2941 tcccttattg ccgaatctgc ttttttggag tatccttaat acatggccta tgaagtaaca
     3001 gcaacaaaaa gaagacctaa gcgttttgaa gatctcgttg gacaagactt tgtctccttc
     3061 acccttaaaa atgcaatttc aagcggaaaa atcgcacact cctatttatt ttctggtccg
     3121 agaggatgcg gaaaaacctc agctgcgcgt attttagcaa aggcgctcaa ttgtgaaaca
     3181 ggcccaacag cttccccttg cgaaatctgc accaactgtc tcgaaatatc taaaggttct
     3241 agcatcgacg ttattgaaat agacggagcc tcaaacacaa gcgtcaacga tgtccgccaa
     3301 ataaaagacg aaatcctctt tccgccaaat gccagcacct ataaaatcta cataatcgat
     3361 gaagtccaca tgctttcaat ttcagcattc aatgcacttc ttaaaacaat agaagaacca
     3421 ccgcactatg ttatttttat ctttgcgaca actgaactac acaaggttcc tgcgactata
     3481 aaaagccgtt gccaacagtt tcaatttaaa ctaggcagtg tagaacaaat aaaaaacctc
     3541 ctcgcggata cagcagaagc agccggtata aaggcagaag aggaagccct gtactggata
     3601 gcaaaagaat caacgggaag ttttcgcgat gcctacaccc tctttgacca agttgcctct
     3661 ttttctgaag gtgagattag ctttgaaaaa ataaaaaaca cactcggtct tgtcggactc
     3721 gaccaaacaa atagcctctt tgaaagttgt gctgaaggaa aaaccgaaac agctctttct
     3781 gaactcgaca caattctcca aagtggcatc acagtcgacc aaacagcagt caactgcaca
     3841 gaatacctgc gctccctctt gcttatcaaa agcggcatta gaaaagaagc ccttcttggt
     3901 tatgctcctc agcgctattc ccaaatatgc atcgactttt ggacacggga acaaatcgaa
     3961 aaggctctcg cgcttttttt acaactctac aaagatttgc ggtattccat taacccacgc
     4021 tatgaactag aacttgcagt ttctcgcctt tgctatctat ccagttatac ctcccccatc
     4081 gaagtaaaaa aagcgctcga ccaaatgcgt aagattttgg cgcagacttc tatagagggg
     4141 aatatagaag ggaaacaagc ggaaggtgaa aacccttctt ttcaaaaacc gatccagcaa
     4201 aaacaagaag tagttattcc tgaaacaaag aacttagaaa aagaagctcc tatgaattct
     4261 atagaacaga gtcttgcaga aaaagaacct ccaaaaaaag cagcaaactt tgctgaattg
     4321 aaagaactcg ttttaaatga actacgcatt aatcattctt ttttagcgcc ctgtattata
     4381 caagctgatt ggaaaaacga agagggtgtt attctttttg aagtagaaaa cagctatgca
     4441 aaaacaacta tagaaaaaaa tcgcccaatt atcacaaaaa ttatacagga tttttggcaa
     4501 gaaaacacca aaatccttgt acagcttgta gaaaagatta cagagcaagc aaacgacgaa
     4561 aagcatcccg aatctgtaga agcggtttgc aatatgttta aaggaaaagt tataagcaaa
     4621 acagaaaccg ctatcttgga aaacactaca aataatgaaa atggagatga tgatgaatcc
     4681 ttttgaaata ttaaaaaatg caggcgccat aaaacaacag cttgaaaaaa cacaagaaga
     4741 actcgattca gttgtcgaga caggctctgc tggcggagct atggtaaagg ttaccatcaa
     4801 tggaaaattt gaaatgctca aggtagaaat agaccccctt actgttgacc cgaaagaaac
     4861 acagatgcta caagacctca ttgttgcggc atataaccaa gcagcgtcaa agatacaaga
     4921 aagtttaaaa gataaggttt cttctttaac cggaggaatg aatatccctg gatttaattt
     4981 ttaatgaacg cacttgatga attaacacaa agtttttctc gccttcctgg tattggaaag
     5041 aaaaccgcct cacgtatttc ccatagcctc ctaaaaatgg accaatcttt tttgacgctt
     5101 tttgcacacc agcttttgag tttacatgag cgcataaaaa tatgctctat ctgtggctct
     5161 tacacggagc aagacccctg ttccatttgc accgatacaa tgcgcgacca agcacttctt
     5221 tgccttgttg aagacccacg agatgtacaa acgattgaag catcccatga attcaacgga
     5281 ctttttcacg ttttaggagg acttatctcc ccccttgacg gcatcggtcc cgaccaactt
     5341 cgcatcatac cacttatcaa tcgcattaaa gaaaataaaa ttaccgaagt tattatcgcg
     5401 acaaacccca ccgttgaggg cgatacaaca gcgctatata tccaacaaat actaaaacct
     5461 tttcccgtaa gtgtttcccg cctcgcttct ggtcttcccg taggagggga tttagaatat
     5521 gcagataaac tcacccttgc acgcagtttt aaaggacgaa cctctcttta agctgagaaa
     5581 ggctctctgt tcaagctctc ttagtttaaa ataaaaaaac cacctctgtt tttaaggtgg
     5641 ttttctctat gttgataagc ctgttattgt agcaagctaa gcacagattg agtgctttga
     5701 tttgcttgcg caagcatagc tgtaccagat tgtgtgagga cttgattttt ggtaagttca
     5761 acaatttcac ttgccatatc agtatctcga atcttagatt cagctgcttg taagttttca
     5821 gaagcaatat ctaaaccttt aatcgtatgt tcaagtcgat tttggtaggc accaagatct
     5881 gctctttgtt tgttaatttg ctttaaagcc tcatcgagcg ttccaattgc acggtttgca
     5941 ttttctggat cttcaagagt tataatcgac tcatcacccg catttcttaa attaagcgcg
     6001 gttgctgtca ttgtcccaat atatgcttca attctttggt ccatattggc accaacgtgg
     6061 aaccacattg atgcagttac aacattttcg ccagtttctt ttgcaaatcg accagtaagc
     6121 atattcattc cattaaattg tgcgctattt gcaatgcggt ctacttctgc taccaattgt
     6181 gaaacttcta cttgaatttg catacgatct tccgaagaat aaataccgtt tgcagactgt
     6241 acagcgagct ctcgcaatcg ttgcacaata tcagttgtgt tttgcaaata tccttctgta
     6301 gcttggatga aactaattgc atttgatgca tttgtggaag cttgatttaa acctcgaatt
     6361 tgacttcgca tt
//
LOCUS       DAOSSW010000089         6327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_11338_length_6327_cov_5.539700, whole genome shotgun sequence.
ACCESSION   DAOSSW010000089 DAOSSW010000000
VERSION     DAOSSW010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11338_length_6327_cov_5.539700"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PKW26_07185"
     CDS             complement(<1..405)
                     /locus_tag="PKW26_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HOQ93386.1"
                     /translation="MSNNPFNGLIARINKKKPSSEPARHAKRAYQKKHFSLSFMVLVL
                     TLFFLFIPLFVIIAYSFNEGKSSSFTGFSLMWYKKLFFESDALWTSLFNSFFIALSSA
                     AVSTVLGTIAAIGINWYKFKGKGYIQTITFLPL"
     gene            complement(405..1292)
                     /locus_tag="PKW26_07190"
     CDS             complement(405..1292)
                     /locus_tag="PKW26_07190"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ93387.1"
                     /translation="MKKKQGIKAFNKNSLGHLYSWPMGLWFTIFFVVPLAIIILYSFL
                     KKGLYGGVEWVFSFNAYKQMFNPNYGIVLMRTLKISFISTLITILIALPCGYAMARSK
                     IQTFLLFLVIIPFWTNSLIRIFAWMSILGNDGFINSLLMQVGLTKEHLQLLYNQNAVV
                     LVSVYMYLPYAILPIFTAIDRFDFSLLEAARDLGSTKMGSMLRVLLPNIKSGVLTAII
                     FTFIPIFGAYTVPLLVGGKDSYMIGNIIVDQVNKTRNWPLAAAFSMIITVLSTFGVLW
                     MMFSSKKEESLKTKKEGGK"
     gene            complement(1289..2431)
                     /locus_tag="PKW26_07195"
     CDS             complement(1289..2431)
                     /locus_tag="PKW26_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ93388.1"
                     /translation="MNGCTVSINNVSKTFGSFQALDDVGFKIEKGEFFSMLGPSGCGK
                     TTLLRIIAGFEFPDEGTVLFDDKNVIPIPPNKRESNTVFQNYALFPHLSIYDNIAFPL
                     KLKKLGKTEIDQRIEKYLNMVQLNAHAYKKPNQLSGGQRQRVAIARALINEPKVLLLD
                     EPLSALDAKLRSNLLIELDNLHDKIGITFIYVTHDQAEALSVSDRIAVMNQGKVLQIG
                     TPYEIYESPATEFVAEFIGETNSLDAVVKECTPIHDKDGQIDEYMCSLEVKELGTTIK
                     ITDYDKTAIGQEVCVTIRPEKVRITLEPPQSSKKDLNVLTGVVEEPVYSGFQSKFYVR
                     LDSGSLFKIYKQHSNYLEDGPEIEWKDRVYVSFSPSDGYVVEEIDR"
     gene            complement(2634..2969)
                     /locus_tag="PKW26_07200"
     CDS             complement(2634..2969)
                     /locus_tag="PKW26_07200"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOQ93389.1"
                     /translation="MIDSALSLNEALLYMLLFGVLIFLTRLFPFALFSKRRMPAFLNF
                     TVSYLPPMIMMILVVYSLKDISFAIKPFAIPHMIGILSCIILHIWKKNAMISIFGSTI
                     LYMLLMKLM"
     gene            complement(2966..3703)
                     /locus_tag="PKW26_07205"
     CDS             complement(2966..3703)
                     /locus_tag="PKW26_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOQ93390.1"
                     /translation="MKQNKITQHTLLHAFKVSIPVFFGYIAIGIPFGLMLTSEGYPWW
                     LAPLMSIVMYAGAGQYVAVGLFASGASIGAALLTMIMVNIRHIVYGLSLIEPLKAYKQ
                     WKLPIIFLLTDETYAVLTSVSEPPNTNRGLFFTLIALFHYSYWILGSLIGAVAGQLIP
                     FSFEGVDFALTSLFIVLLIEQVRKTKNSLPPIIGFIVSVFALFVFGPSRMLIAAIAFG
                     IALISFLQKPLLAAEAKQKAKKMEEKP"
     gene            3763..4635
                     /gene="purU"
                     /locus_tag="PKW26_07210"
     CDS             3763..4635
                     /gene="purU"
                     /locus_tag="PKW26_07210"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOQ93391.1"
                     /translation="MKLLIEGIFMQKYTLTVSCPDKSGLIASITNDIASANGNIINLA
                     QHTAVDIDTFFCKVDFTPVNGFDLAVFKAQFESSAQKYAMKWRLYDWNKKSRMAVLVS
                     KTSHCLYEVLLKHQDGQLNCDIPLVISNHPDLSHIATDFHIPFFKVDPAKGKAAYEAD
                     MQKLLEEYDIDVICLARYMQILSPEFTRAWEHKVINIHHGFLPAFKGAKPYHQAWQKG
                     VKIIGATAHFANEDLDQGPIIYQDIVRVNDTKSIEEFVLVGKDVERKVLVEGLKRYFN
                     HNIFLAGNRTFILE"
     gene            4651..5907
                     /locus_tag="PKW26_07215"
     CDS             4651..5907
                     /locus_tag="PKW26_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOQ93392.1"
                     /translation="MNAREISPWVYALHADIKDAPYFEGFWPIPHGVSLNSYIVKGDK
                     VALIDLYRDWEGAPEKMKAQMASIGIDLCDIHYLVLNHLESDHSGFLKTFRELCPNAK
                     IVTTKKGAALVENFCKAVNEGESDFQNAHVQVVKTGDSIDLGDAVVLHFTETPNVHWP
                     ETMMTYESKSKTLFSCDGFGSYGCLGTEENPTRIFDDECSEEELQFFETEALRYYANI
                     VASFSSFVKQAIEKLSGIEVKVVAPSHGLVWRKNPQRIIDDYIRYASYNTGGELEKEV
                     CVIWGSMYGNTKLGLDAVIEGIEAEGVKVKVIEIPSTDPSFVLAHAYKAKGLVIAMPT
                     YEYKMFPPMAYVLNLFNRKHFSGKTALRIGSWGWIGGAKKEYDEAVLPLKWDEVESYE
                     WQGLPSESDKASLKERGRELAKRIKA"
     gene            5918..>6327
                     /locus_tag="PKW26_07220"
     CDS             5918..>6327
                     /locus_tag="PKW26_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOQ93393.1"
                     /translation="MKKPLFVAVNSRYNHTNLAIRSIASYCAVHSKRQLSILEASIAE
                     FPTNILRSIYERLKEESDRDGQIKQELIIFSVYIWNASLVYEVANELKKILPRALIGF
                     GGPEVSYQAEKVFFENPSLDFIVKGEGEETVKELV"
BASE COUNT         2005 a         1149 c         1355 g         1818 t
ORIGIN      
        1 aaggggtaaa aacgtaatgg tttgaatata gcccttgcct ttgaacttat accaattaat
       61 gcctattgct gctatcgtcc ccagaacggt ggatactgcc gcagagctaa gggcaataaa
      121 aaaactattg aacaaagaag tccataaggc atctgattca aaaaagagct ttttatacca
      181 catgagagaa aagcccgtaa aggaggaact ttttccctcg ttaaatgaat aggcaatgat
      241 gacaaataag ggtatgaata aaaagaagag tgttaaaaca agcaccataa atgagagaga
      301 aaaatgcttt ttttgatagg ctcgttttgc gtgtcgcgca ggctctgaac ttggcttttt
      361 tttgtttatg cgtgcgatga gaccattgaa gggattgttg ctcattattt tcctccctct
      421 tttttggttt ttagactttc ttcttttttg gaagaaaaca tcatccaaag cactccaaag
      481 gtacttagga cggtgataat catagaaaaa gcagctgcca agggccagtt acgtgtttta
      541 ttgacttggt ctacgatgat atttcctatc atataggaat cttttccacc gacaaggagt
      601 ggcacggtgt acgcgccaaa gatggggata aaggtaaaga taatcgccgt taagacgccg
      661 cttttgatat ttggcaaaag gactcgtagc atggagccca tttttgtcga cccgagatct
      721 cgagctgctt ccaaaagcga aaagtcaaag cggtcaattg cggtaaatat gggcaaaatt
      781 gcatagggga gatacatgta taccgaaaca aggacgacag cattttggtt gtagagcagt
      841 tggagatgct cttttgtaag accgacttgc atgaggaggg agttgataaa gccatcgttt
      901 cccaagatgc tcatccaagc aaagatgcga ataagggagt ttgtccaaaa gggaatgata
      961 actaaaaaaa gcaaaaaggt ttgtatttta cttcttgcca ttgcatagcc acagggaagg
     1021 gcgatgagta tggtgataag cgttgaaata aaagaaattt taagagtccg catcaagaca
     1081 atgccgtagt tggggttaaa catttgtttg taagcattga agctaaaaac ccactcgacg
     1141 ccaccgtaga gtcctttttt caaaaaacta tagagaatga taatggcaag cggcacgaca
     1201 aagaaaatcg taaaccacaa acccatgggc catgagtaga gatggccgag agagttttta
     1261 ttaaaagcct ttataccttg cttttttttc atctatctat ttcctcgact acatagccat
     1321 cactggggct aaatgaaacg tagactctat ccttccattc gatttctgga ccatcttcga
     1381 gataatttga gtgttgcttg tatattttga acagagagcc agagtcgagg cgcacataaa
     1441 actttgactg aaagccagaa taaacaggct cttcaacaac accggttaag acgtttaagt
     1501 ctttttttga gctctgtggt ggctcaagcg taatccgtac cttttcgggg cgaatcgtaa
     1561 cacaaacctc ttgtcctata gctgttttgt catagtcggt tatttttatt gttgtgccaa
     1621 gctcttttac ttcgaggcta cacatgtatt cgtcgatttg cccatcttta tcatggatgg
     1681 gagtgcattc ctttacgaca gcgtcaaggc tatttgtctc accaataaac tcggcgacaa
     1741 actcggttgc agggctttcg tagatttcgt agggagtgcc tatttgtaaa accttccctt
     1801 ggttcatgac agcaattctg tcagaaaccg acagggcctc ggcttggtcg tgggttacat
     1861 agataaaggt aataccgatt ttatcgtgta aattatcaag ctcgatgagg agatttgagc
     1921 gaagttttgc atcgagtgct gacaaaggct catctaaaag caaaaccttt ggctcattta
     1981 taagtgcacg ggcgatagca acacgttgcc tctgtccgcc agaaagttgg tttggctttt
     2041 tataggcatg tgcattgagt tgtaccatat tgaggtattt ttcgatcctt tgatcgattt
     2101 cagttttccc cagttttttt agcttaagcg gaaacgcaat attatcatag atagaaagat
     2161 gcggaaataa agcatagttt tgaaaaacag tatttgattc tcttttgtta ggaggaattg
     2221 gaatgacgtt tttatcgtca aaaagtaccg ttccttcatc tggaaattca aaaccagcaa
     2281 ttattcgcag taaggttgtt ttaccgcaac cagaaggtcc aagcattgaa aagaactcac
     2341 ccttttcaat tttgaacccc acatcgtcca atgcttggaa cgatccaaat gtttttgaaa
     2401 cattattgat tgatactgta catccgttca attccgccgt accctcttta aatatatatt
     2461 ctatcttgta caatgatgaa aaaagccttg cgtgtcaagt atttgataaa agaaaatttt
     2521 ctattttgaa taaaaaaaac cattataaag agtattatta tagtaagaac ttgttatttt
     2581 acgagtttta gtcgcataaa aaaatggaaa tattttttaa tttcgatata attttacatc
     2641 agtttcatta atagcatata taaaatagtt gagccgaaaa tacttatcat tgcatttttc
     2701 ttccatatat gcaggataat acaagagaga attcctatca tatgaggaat agcaaagggc
     2761 tttattgcaa aagaaatatc tttgagcgag tatacaacaa gaatcatcat aatcatggga
     2821 ggaagatagg aaacagtgaa gtttaaaaag gcaggcatcc ttcgtttaga aaagagcgca
     2881 aagggaaaaa ggcgagtgag aaaaattaaa acgccaaata gaagcatgta aagtaaagct
     2941 tcgtttaggc tcagagcaga atctatcatg gcttttcctc catttttttt gccttttgtt
     3001 ttgcttctgc agcaagaagt ggtttttgca aaaaacttat gagggcaatt ccaaaggcaa
     3061 tcgctgcgat aagcattcgc gagggtccaa atacaaataa ggcaaaaaca gaaacaataa
     3121 aaccaatgat aggtggcaag ctatttttgg tttttcttac ttgttctatt aagagtacaa
     3181 taaacaaact tgtaagagca aaatcgacac cttcaaagga aaatgggatg agctgacctg
     3241 caactgcacc gatgaggctt ccgagtatcc agtaagaata atggaagaga gcaataaggg
     3301 taaaaaagag cccacggttt gtatttggcg gttcgctaac tgaagttaaa acagcatagg
     3361 tttcatcggt gagtaaaaaa ataataggta gtttccattg cttgtatgct ttcaaaggct
     3421 cgatgagaga aagaccgtat acaatatgcc ttatattgac cattatcatg gtgaggaggg
     3481 cagctccaat gcttgctcct gaggcaaaga gtccgacggc aacgtactgt cctgccccgg
     3541 catacatgac gatgctcatg aggggggcga gccaccaagg atagccttct gatgtgagca
     3601 tgagcccaaa gggaattccg atggcaatat agccaaaaaa aacgggaatt gaaactttaa
     3661 atgcgtgtaa aagagtatgt tgtgtaattt tattttgctt cattaagcat tcattatacg
     3721 tgcttgccct cgacctttca agatgttata ctaaaatata aaatgaaatt attaatcgaa
     3781 ggaattttta tgcaaaaata taccttgacg gtgagctgcc ccgataaaag cggcttgata
     3841 gcatctatta caaatgatat tgcttcggca aatggcaata tcatcaacct cgctcaacat
     3901 accgcggttg atattgacac ctttttttgt aaggtagatt ttacaccagt aaatggtttt
     3961 gacttagctg tttttaaagc gcagtttgaa agctctgctc aaaaatacgc aatgaaatgg
     4021 cgtttgtacg attggaataa aaaaagtcgc atggctgtct tggtttcaaa aacaagccat
     4081 tgtttatatg aggtgctttt aaaacatcaa gacggacaac ttaactgcga cattcccttg
     4141 gttatatcaa accatcctga cttgtcacat attgcaacag attttcatat tccttttttc
     4201 aaagttgatc ctgcgaaggg aaaggctgcc tatgaggcgg atatgcaaaa attattggaa
     4261 gagtatgata tcgatgttat ctgtcttgcc cgctatatgc aaattttaag tccagaattt
     4321 acccgcgctt gggaacataa ggttatcaat attcaccatg gattcttgcc tgcctttaag
     4381 ggtgcaaagc cctatcacca agcctggcaa aagggtgtaa aaattattgg agcaacagcg
     4441 cattttgcaa acgaagacct tgaccaaggt cccattattt atcaggatat tgtacgtgtc
     4501 aatgatacca agtcgattga agaatttgtc ctcgttggca aagatgttga acgaaaggtt
     4561 ttagttgaag gcttaaaacg ctattttaat cacaatattt ttcttgcagg aaacagaacc
     4621 tttatattgg aataaggaga ttaagagaaa atgaacgcac gtgaaatttc gccatgggtt
     4681 tatgccctcc atgcagatat aaaagacgcc ccctattttg aaggtttttg gccgattcct
     4741 cacggagtct cgctcaattc atacatcgta aaaggcgata aggttgcact tattgacttg
     4801 tatcgtgatt gggaaggtgc tccagaaaaa atgaaagcac aaatggcttc gataggtatc
     4861 gacctttgtg atattcacta ccttgtttta aatcacttag aaagcgacca ctcaggtttt
     4921 ttaaaaacat ttcgcgagct ctgtccaaat gcaaaaatcg taaccactaa aaaaggagca
     4981 gccctcgtcg aaaatttttg taaggcggta aatgagggag aaagcgattt tcaaaatgct
     5041 catgtacagg tggtaaaaac aggagatagc attgacctcg gtgatgcggt tgtccttcat
     5101 tttactgaaa cgccaaatgt acactggcct gaaacaatga tgacttacga atcaaaatcg
     5161 aagacgcttt tttcttgtga cggctttggt tcctacggct gccttggcac tgaagaaaat
     5221 ccgacacgta tttttgatga tgagtgcagt gaagaagagc ttcagttttt tgaaacagaa
     5281 gccctgcgtt attatgcaaa cattgttgca agtttttcaa gctttgtaaa acaagcaatc
     5341 gaaaaacttt caggcattga agtgaaggtt gttgcaccga gccatggttt agtgtggaga
     5401 aaaaatccac aaagaattat cgatgactat attcgctatg cttcctataa tacaggaggg
     5461 gagttagaaa aagaagtatg cgttatttgg ggctcgatgt atggaaatac aaaactcggt
     5521 cttgatgcgg tcatagaagg cattgaagcg gaaggcgtaa aagtgaaggt cattgaaatt
     5581 ccttcaacgg atccatcctt cgttcttgcc catgcctaca aggcaaaggg acttgtaatt
     5641 gcaatgccca cctacgaata taaaatgttt ccgcctatgg cctatgtatt aaatcttttt
     5701 aaccgaaaac atttttctgg gaaaacagct cttcgaattg ggagctgggg ttggatcggc
     5761 ggcgcaaaaa aagaatacga cgaagctgtc cttccgttaa aatgggatga agttgaaagc
     5821 tatgaatggc agggacttcc aagcgaaagc gataaggcaa gtctcaaaga aagaggaaga
     5881 gaactcgcaa agcgcataaa ggcttaaaca gagtcttatg aaaaagccgc tctttgttgc
     5941 tgttaattca cgatacaacc acaccaattt agctatccgc tctatcgctt cctattgtgc
     6001 agtccatagc aaaagacagc tctctatttt ggaagcaagc atagctgagt tcccgacaaa
     6061 tattttgcgc agtatatatg agcgtttaaa agaagagtca gatagagacg ggcaaataaa
     6121 acaagagctt attatttttt ctgtatatat ttggaatgct tctctcgtct atgaagttgc
     6181 aaatgaacta aaaaaaattc ttcctcgagc tctcatcggt tttgggggac cagaagtttc
     6241 ttatcaagca gaaaaagtct tttttgaaaa tccatccctc gactttattg taaaggggga
     6301 gggagaggaa acggttaaag aacttgt
//
LOCUS       DAOSSW010000090         6224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_11582_length_6224_cov_8.398768, whole genome shotgun sequence.
ACCESSION   DAOSSW010000090 DAOSSW010000000
VERSION     DAOSSW010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11582_length_6224_cov_8.398768"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="PKW26_07225"
     CDS             complement(<1..1376)
                     /locus_tag="PKW26_07225"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002687980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOQ93394.1"
                     /translation="MAYFYEEPSHTFAEYLLIPGYSSADCIPDKVNLKTPLVRFKKGE
                     KPKLSMNIPLVSAIMQSVSNDRLAIALAKEGGVSFIFGSQSIEDQAEMVSKVKNHKAG
                     FVTSDSNIKPNQTIQEAIALTEKTGHSTIAVTEDGTANGKLLGILTSRDYRIGFTDLD
                     EKVERYMTKFSDLIKGENGISLNEANDLIWQHKLNSLPIIDTNENLKYLVFRKDYASH
                     EAHPLELLDDQKRYVVGAGINTRDYMERVPALVKAGADVLCLDSSEGFTEWQSRALAD
                     IHKSFGPDVKVGAGNVVDREGFLFLAEAGADFIKVGIGGGSICITREQKGIGRGQATA
                     VIEVAKARDEYYKKTGIYIPICSDGGIVHDYHMTLALAMGADFVMLGRYFARFDESPT
                     NRLNINGNYVKEYWGEGSNRARNWQRYDLGGTSTLSFEEGVDSYVPYAGSLHDNVGMT
                     MSKIRHTM"
     gene            complement(1476..2390)
                     /gene="metA"
                     /locus_tag="PKW26_07230"
     CDS             complement(1476..2390)
                     /gene="metA"
                     /locus_tag="PKW26_07230"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HOQ93395.1"
                     /translation="MPIKIQADLPAREILEKENIFVMTDQRAEHQDIRPLKIAIVNLM
                     PTKIATETQILRLLGNSPLQVEISLVHMESHVSKNTSQEHLERFYINPQELYKKKYDG
                     IIITGAPVEQIDFFDVDYWDELCQIMDWAKTHVFSSLFICWGAQAGLYHYYGIEKTNL
                     EKKLFGIYPHYKTTVGEPLLRGFDDIFPAPQSRHTKVDEDAVKNHEKLLVLAEGKEAG
                     LLLAKSLDNKHIFMTGHLEYDTETLGNEYRRDLDKGLPIEMPLNYYPDNDPWQKPRST
                     WRSHAHLFFSNWLNYYVYQETPYEFTRE"
     gene            2439..3332
                     /locus_tag="PKW26_07235"
     CDS             2439..3332
                     /locus_tag="PKW26_07235"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ93396.1"
                     /translation="MRGKDKRAAVKKLKSLVFTQKAEPIPFRNKIESQFSVFFLPNNV
                     ERTEKNYNSVVCDLLRPVAFAKERLIIYIHGGSFVGGSRASWRSFASSLAHASATELL
                     LPEIRLAPEYPFPSALDDIEVVFTALSKDTRDIILCGDGTGGTIALSFALFAKKTLRD
                     KIKKLVLFSPFLDFSPDSALLQIKKISDALISTESIRRMADTYTYASNLANPMVSPLC
                     ASEDDLKNLKQVYIQMGEDEIILPDARRFATKLGYAAVPHILDVWPGMIHFFQMADDC
                     IPEAHKAIEKVAAFIKSNEES"
     gene            3350..4735
                     /locus_tag="PKW26_07240"
     CDS             3350..4735
                     /locus_tag="PKW26_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOQ93397.1"
                     /translation="MEIVSPAGNVEKLYYAWSYGADAAYIGLKSFSLRIKADNFYEDE
                     YNKVIELKKQFPGKKLFCALNISFHNRDLDNFIREIDYFKQYPIDSFIVSDMGVINII
                     QKEFPHAALHLSTQANCINREAVKVYKDLGFKRVVLGREASLDDIRRIKDENPEMELE
                     VFAHGAMCIAYSGRCLLSAYMTGRSANSGFCSHSCRWDYKLLEEKERPGEYFPVYEGD
                     DFTAILSSKDICMIDHLDDMKKAGVDSLKIEGRMKSLYYAALVTRSYRKAVDALEGKI
                     ASEEAAPYIDELYKTNHREFATGFFYSQADANKTTRGETKSDWELVGTIGKALSEEES
                     EALFTKAEQESMALQNELDAMHPEAKKARLKDLEMHPEKRYRPLEKKEGFRLYAFTAL
                     NKLTEDVIIEYIGPDIISISDTSFSFVAPEDASLLAWVTFNNDCLIYTNKKIAEGFIV
                     RKKNEKIFNKN"
     gene            4710..5462
                     /locus_tag="PKW26_07245"
     CDS             4710..5462
                     /locus_tag="PKW26_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93398.1"
                     /translation="MRKFLTKISFCIILFFIFAIPLFSSSIKYEMEHEISGKTGTVSG
                     IIIDLFNPFCSFTDTMYLKLFDANYFSTFAGFHFIQGAASFTLSPTWYFFKSNYFDAG
                     VLFLYHFNAFYNLAIDQDFFYGLSFRAGTPNLVECLVDVSFMQKYTTIHSIAKSVPFF
                     LNNGMAFKLKVKKQFTEKLTTSISLASYDEFYYPLFLFPIYALCFQYRLNEEWKLSTE
                     LALCYTDQFTLTSHMNTAVFKLGVSYMISKEQ"
     gene            5465..>6224
                     /locus_tag="PKW26_07250"
     CDS             5465..>6224
                     /locus_tag="PKW26_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOQ93399.1"
                     /translation="MKKYSLLFLLSIFLCFALLFTSCKYGLEQFLFRKYDVNNRSTSL
                     EKIKSPFEGSQPQNYKILLLADLHFGKGKTNVKEKLFSYLDELKSEGKELPSFALVLG
                     DIADTGSDFEFNQYKILLDDLETRYGITKSYGIVGNHDLYNTGWLEWEKAVYPHKSFY
                     WFSTQKFSWYFLDSGNGSLGSKQLNSFVTALKKDPSPKFVFIHYPLYRDDIFYFSLGD
                     PRERAVLIDACAKYNVRAVFSGHDHYGSRFDYGPF"
BASE COUNT         1766 a         1143 c         1263 g         2052 t
ORIGIN      
        1 cacatagtat ggcgaatttt actcattgtc ataccgacat tgtcgtgcaa gctgccagcg
       61 tagggcacgt aggagtcaac gccttcttca aaagaaagcg tgcttgttcc gccgaggtca
      121 tagcgctgcc agtttcgagc tctatttgaa ccctctcccc agtattcttt tacataattt
      181 ccgttaatat tgagtcggtt tgtggggctt tcatcaaagc gggcaaagta gcgaccgagc
      241 ataacaaagt cggctcccat cgcaagggcc aatgtcatgt gataatcgtg aacgatgcca
      301 ccgtcagaac aaatggggat atagatacct gtttttttat agtattcgtc tcgcgccttt
      361 gccacttcaa taacagcagt cgcttgcccg cgtcctattc ctttttgttc acgggtgata
      421 caaatggaac caccaccaat gcctactttt ataaaatcag cgccagcttc ggctaaaaat
      481 aaaaagcctt ccctgtcgac aacatttcct gcgcctactt ttacatctgg tccaaagctt
      541 ttgtgaatat cggcaagggc tcgtgattgc cactcggtaa atccttctga ggaatctaag
      601 cacagtacgt cggcacctgc tttgacgagg gcagggactc gctccatata atcgcgggta
      661 tttataccag cgccgacaac atagcgtttt tggtcatcga ggagctcaag gggatgcgcc
      721 tcatggcttg cataatcttt tctaaagacg agatatttga ggttttcgtt tgtatcgata
      781 atcggaaggg aatttagttt atgttgccaa ataaggtcgt ttgcttcgtt taagcttatg
      841 ccgttttctc ccttgatgag atcagaaaac tttgtcatat agcgttcaac tttttcatcc
      901 aagtcggtaa atcctatgcg ataatcacga ctcgttaaaa tgccgaggag ttttccattg
      961 gctgtgccat cttctgttac cgcaatcgtt gagtgccctg ttttttcagt tagcgcaatt
     1021 gcttcttgga tcgtttgatt tggtttaata tttgagtcgg aagttacaaa acccgcctta
     1081 tgatttttta cttttgaaac catttcagct tggtcttcta tgctttggga gccaaaaata
     1141 aaggaaacac cgccttcttt tgccaaggcg attgcaagcc tgtcgttcga tacagattgc
     1201 ataattgccg aaacaagcgg tatgttcatg ctgagctttg gtttttcacc ttttttgaag
     1261 cgaacgagtg gcgtttttaa gttaacttta tcggggatgc agtcagcaga tgaatagcca
     1321 ggaataagga gatactctgc aaaagtatgg gaaggttctt cgtaaaaata agccataaaa
     1381 tgtccttttt gttttattat atagatattg tttcatatat gcaagattga tatgaaaatc
     1441 tgagaaaaaa gtatttctgt gttgcgacca gttttttatt ctctagtaaa ttcgtagggt
     1501 gtttcttggt agacgtagta gttgagccag tttgagaaaa aaagatgggc atgacttcgc
     1561 caggttgagc gaggtttttg ccaggggtcg ttgtctggat aataatttag gggcatttct
     1621 atggggagcc ccttgtctaa atctcgacgg tattcgtttc cgagtgtttc tgtgtcgtat
     1681 tcaagatgac cggtcataaa aatatgcttg ttatcaagag actttgcaag gaggagacca
     1741 gcttcttttc cttccgccaa aacaaggagt ttttcgtgat tttttaccgc atcttcatcc
     1801 acctttgtgt ggcgcgattg cggggcagga aagatatcgt caaatccgcg aagcagcggt
     1861 tctccgacag tcgttttata atgcgggtaa atgccaaaga gttttttttc gaggtttgtt
     1921 ttttctatgc cataataatg atataaacct gcctgcgctc cccagcaaat aaaaaggctt
     1981 gaaaatacat gggtttttgc ccaatccatt atttggcaaa gctcatccca gtaatctaca
     2041 tcaaaaaaat ctatttgttc gacgggtgcc ccagtgataa taatcccgtc gtattttttt
     2101 ttgtatagtt cttgggggtt aatataaaag cgttctaaat gctcttgact cgtgtttttt
     2161 gagacatggc tttccatgtg cacgagagaa atttcaactt ggagggggga atttccaagc
     2221 agccgtaaaa tctgcgtttc agttgcaatt ttggtaggca tgagatttac tatggcaatt
     2281 tttaagggtc ggatatcttg atgttccgcc ctttgatctg tcataacaaa gatattttct
     2341 ttttcaagta tttcgcgtgc aggtaagtct gcttgtattt ttattggcat aatagggata
     2401 gtatagcatt tttattgatt ttttgctaca cttttactat gcgagggaaa gataaaaggg
     2461 cagcggtaaa aaagcttaaa tcgctagtgt ttacgcaaaa agctgaaccg ataccatttc
     2521 gaaataaaat agaatctcaa ttctctgttt tttttctacc gaataatgta gaaagaacag
     2581 aaaaaaatta taactcggta gtttgcgatt tgcttagacc agttgccttt gcaaaagagc
     2641 gccttatcat ctatatacat ggaggttcct ttgtcggtgg ctctcgtgcc tcttggcgta
     2701 gctttgcttc ctctcttgca catgcctccg cgactgaact tttattgcct gaaatccgtc
     2761 tcgcgccaga gtatcccttt ccttctgccc tcgatgatat agaggtcgtt tttaccgcac
     2821 tttcaaaaga tacgcgagat atcatcctct gtggagacgg aactggtggc acaatagcgc
     2881 tttcttttgc gctttttgca aaaaaaacac tgcgtgataa aataaaaaag cttgttttat
     2941 tttcgccttt tttggatttt tcacctgatt cagccttatt gcaaataaaa aaaataagcg
     3001 acgccctcat ttcaactgag agcattcgac gtatggcaga tacctatacc tatgcttcaa
     3061 acttggctaa tcctatggtt tctcctctct gtgcttcgga agatgactta aaaaatctca
     3121 agcaagtata tattcaaatg ggagaggatg aaattattct gcctgatgcg cggcgttttg
     3181 caacaaaact tggctatgca gctgttcctc atatcctcga tgtttggcct ggtatgattc
     3241 acttttttca aatggcagat gattgtattc cagaggcaca taaggcaata gaaaaagttg
     3301 ctgcctttat taaatccaac gaagagtctt agagcttgta aaggtctgta tggaaatagt
     3361 atctcctgcg ggaaatgtag aaaaattata ttatgcttgg tcctatggcg ctgatgcggc
     3421 ctatattggg ctgaagtctt tttcacttcg catcaaggct gataattttt acgaagatga
     3481 atacaataag gttatagaac taaaaaaaca gtttccagga aaaaaacttt tttgtgcgct
     3541 caatattagc tttcataatc gagacctcga caattttata cgtgagatag attattttaa
     3601 gcagtatcct atcgactctt ttattgtctc tgacatgggt gtgataaata taattcaaaa
     3661 agagtttcct catgctgcct tgcatttaag tacgcaggca aattgtatca atcgagaagc
     3721 ggtgaaggtg tacaaagacc tcggtttcaa acgggttgtt ctagggcgtg aagcttctct
     3781 cgatgatatt cgtcggataa aagatgaaaa tcctgagatg gaacttgagg tttttgccca
     3841 tggtgcaatg tgcatcgctt actctgggcg ctgtcttttg agcgcttata tgacggggcg
     3901 cagtgcaaac tctggttttt gctctcattc ttgtcgctgg gattacaagc ttttggaaga
     3961 aaaagagcgt ccaggcgaat attttcctgt ctacgaaggg gatgacttta ccgctatttt
     4021 atcaagtaaa gatatatgta tgattgacca cctcgatgat atgaaaaaag ctggagttga
     4081 ttccttaaaa atagaaggca ggatgaaaag tctctactac gcagcgctgg taacgagaag
     4141 ttaccgaaag gctgttgatg cgcttgaggg aaaaattgca agtgaggaag ctgctcctta
     4201 tatcgatgag ttatacaaaa caaatcaccg agaatttgct acgggatttt tctactcgca
     4261 agcggatgca aataaaacca caagagggga gacaaaaagc gactgggaac ttgtcggcac
     4321 aatcggcaaa gctctctctg aggaagaaag cgaggctctt tttacaaagg cagaacaaga
     4381 gtcaatggct ttgcaaaatg aacttgatgc gatgcatcct gaggcaaaaa aagctcggct
     4441 aaaagatttg gaaatgcatc cagaaaaaag ataccgtccc ctagaaaaaa aagagggttt
     4501 taggctgtat gcctttactg ctttgaataa gttgacagag gatgttataa tagaatacat
     4561 tggaccggat attatctcta tttcagatac gagtttttct tttgttgcac cagaagacgc
     4621 ctcgctttta gcttgggtta ccttcaataa tgattgctta atttacacaa ataaaaaaat
     4681 tgctgaaggt tttattgtga ggaaaaaaaa tgagaaaatt tttaacaaaa attagttttt
     4741 gtattattct tttttttatt tttgcaattc cacttttttc ttcctcaata aaatatgaaa
     4801 tggaacatga aatctcagga aaaacaggaa cagtttctgg gataataatc gatctattta
     4861 atcctttttg tagttttact gatactatgt atctaaaatt atttgatgca aattattttt
     4921 caacatttgc aggctttcat ttcatacaag gagctgcaag ttttacgctt tctccgactt
     4981 ggtatttttt taaatcaaac tattttgatg ctggtgtttt atttttgtat catttcaatg
     5041 cgttttataa tttagcaatc gatcaagatt tcttttatgg actttccttt cgggctggta
     5101 ctccaaacct agtcgaatgt ttagtcgatg tttcttttat gcaaaaatat actactattc
     5161 attctattgc aaaatctgtt ccattttttc taaataatgg aatggctttt aagttaaaag
     5221 taaaaaaaca atttacagaa aaacttacta cttctatttc gcttgcttca tacgacgaat
     5281 tttattatcc cttatttctt ttcccaatct atgctctctg ttttcaatat cgattgaatg
     5341 aagaatggaa gctttcaact gagctagcac tttgttatac tgatcagttt acactcactt
     5401 cacacatgaa tactgctgtg ttcaaacttg gtgtatccta tatgatttca aaggagcaat
     5461 gagtatgaaa aaatatagcc ttcttttttt attaagtatc tttttatgct ttgcgcttct
     5521 ttttacttct tgtaaatatg gcttggaaca atttttgttt cgtaaatatg atgtgaataa
     5581 taggagcacg agtttagaaa aaatcaaatc tccttttgaa ggctcacaac ctcaaaacta
     5641 taagatactt cttttagcag acttacattt tggaaaagga aaaacaaatg taaaagaaaa
     5701 attgtttagc tatcttgatg agttaaaaag tgagggaaaa gagcttccat cttttgcact
     5761 tgtgcttgga gacatagcag atacaggaag tgattttgaa tttaatcaat ataaaatact
     5821 actcgatgat ttagaaactc gctatggcat aacaaaaagt tatggaattg taggtaatca
     5881 cgatctttac aatactggtt ggcttgaatg ggaaaaagcg gtatatccac ataaaagctt
     5941 ttattggttt tctacacaaa agttttcttg gtatttttta gattcaggaa atggcagtct
     6001 tggttcaaaa cagctcaatt cttttgtaac agctttgaaa aaagatccct ctccaaaatt
     6061 tgtttttatt cattatcctt tgtaccgcga tgatattttt tatttttcac ttggtgatcc
     6121 ccgcgaacga gctgttttaa ttgatgcttg tgcaaaatat aatgtacgag cagttttttc
     6181 tggtcatgac cattatggca gccgctttga ttatggtccc ttcg
//
LOCUS       DAOSSW010000091         6162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_11706_length_6162_cov_6.134600, whole genome shotgun sequence.
ACCESSION   DAOSSW010000091 DAOSSW010000000
VERSION     DAOSSW010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11706_length_6162_cov_6.134600"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PKW26_07255"
     CDS             <1..406
                     /locus_tag="PKW26_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93400.1"
                     /translation="EEIIALKDITLLVEPRFKTKNLGIAFSLFNIPPQVAQKLFYIEN
                     PLGVNVSLFYNTFANLGFQGEIGVHTTFSAPESTFALSQDDLAIQIAPYIKADIGGGS
                     LSAAIKCKVLNFTSLDGAIENTSLSIGYKAQL"
     gene            403..1182
                     /locus_tag="PKW26_07260"
     CDS             403..1182
                     /locus_tag="PKW26_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93401.1"
                     /translation="MIKKKIFICIIFSFISFLPVFSLDFGFTSHFLDFSSAQNEPSFA
                     LSFDITEEIASGIEIGAKILQSNKHNYTAFSTADFAFSFFHLGGGIMYDIRDALISPG
                     FIVDARVDLKKESFLGLSYYASFSPENLAENISQNGKIYLSSKNTNASLEAFADITYV
                     QIQEVKHNLILTSKTEIMGFDEKTFIAVGLLFSSELSINTDMLLELDLAFRLGGKIAF
                     NFKKWGDYFFTYESNLFHLSEKIQNIPSFNIHCGARFRIDN"
     gene            1186..1950
                     /gene="recO"
                     /locus_tag="PKW26_07265"
     CDS             1186..1950
                     /gene="recO"
                     /locus_tag="PKW26_07265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOQ93402.1"
                     /translation="MSRHWQTEALVLQVKSMGEDNRSVALLTRSRGLIWATLYGGPKS
                     KMRALVSPFHCGQIYLYTDEVKQATKISDFAIHSYRPEIRENLFKTCAANLCSELVIK
                     THGGGEIEQLWLYVNSFLDGLCLVNEEAARHALLRFIWRFLNTMGMQPDPCECASCGT
                     SVCSIYNTQKVFFSLAESSFYCAHCSSLGSESKGFFLHKEVVDYLRIITTEAPRVSRD
                     IVLEEAFFYETQDFLVYILQNFSGQKFNSLDVLLKV"
     gene            2114..2806
                     /gene="speD"
                     /locus_tag="PKW26_07270"
     CDS             2114..2806
                     /gene="speD"
                     /locus_tag="PKW26_07270"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOQ93403.1"
                     /translation="MSYAHSEKSQIDYLEYIDEEYDSKRLQTIIEEVTRIIDAKLVNI
                     STQDYDPRGASVVALINEDHPAEYVKESLVCGHLDKSHITAHTYPEYNSRTGLATFRV
                     DIDVSTCGMISPLRALHYLIDSFDSDIVLMDYRVRGFTRDTRGNKIFIDQDISSIQDY
                     IDEDVLADYLAYDINILSDTIFHTKMRKKNLNLDDYLFGDEIHELTETERSRVKKMLT
                     KEIQEIFECANL"
     gene            2791..3729
                     /gene="speE"
                     /locus_tag="PKW26_07275"
     CDS             2791..3729
                     /gene="speE"
                     /locus_tag="PKW26_07275"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOQ93404.1"
                     /translation="MRKFIRLFSKKRTMHFEHTSSGGGWFFDVEKKLQSAKTQWQKID
                     VVKTKEFGKALLLDGITQLTEAQEYQYHEPMAHVPLLAHKNPKKALVIGGGDGALAQE
                     ILRHPSIESLDFVELDEGVINFCKKHLPELGGDAFNDERVKLHIQDGRAFVENAEKEG
                     KTYDAIFMDMTDPEGPCIALYTKEFFEIVSSLLKDDEAFFIMHAESPDLRLKTFQRIM
                     HTLKAVFPSVQEMVSHVRMYGGMWTWAIASKGFNPKNFDAKKIPAVIEKRGLKNLKII
                     SSETWESFFRLWPVYKALLDEEGEIATDANPGYAME"
     gene            3744..4736
                     /locus_tag="PKW26_07280"
     CDS             3744..4736
                     /locus_tag="PKW26_07280"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOQ93405.1"
                     /translation="MAKIKLAVIGYGNIGRGVLEAIKKNSDMELACILSRSPDRVRAE
                     IGGSYPIYASDDFEKLQEADVAILCGGSKSDLPEQGPVYAQYIHTLDSYDNHSEAYAY
                     YQTMDEAAKKTEHLSCISIGWDPGTFSVQRVLGNAFIPGAKTYGFYGTSEKGGLSMGH
                     SDALRRIAGVLDARQYTHAISESIEKVRRGENPSLKPGDMHWRECFVVAKEGADTAKI
                     EEAIKTMPGYFAGYKTLVHFVSKEELETKHQGFPHDGLVIGAGTTGSGNPARIEYTNI
                     WGSNSEATASILVAHARAVYRLAKEGKTGAITALDIAPAYLSPQSREDLIKNFL"
     gene            4766..6070
                     /locus_tag="PKW26_07285"
     CDS             4766..6070
                     /locus_tag="PKW26_07285"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOQ93406.1"
                     /translation="MNKINKSAVSPSAILDAAKKTATPFYIYDEASIRERCQDMLAMP
                     NAFGLTVRYALKANSTKRLIQIIDKEGLHFDASSLNEVKRAIQAGIKPEKILLTTQEV
                     ATDAELLDIQALLKKGFQYNACSLTQLKKIAPFASKENISIGLRIHPGVGSGESVTRN
                     TGDKYSSFGIHLSNIDEAKAIIKEYGLDIKRIHVHIGSGGDPEAWKENIFRELFLIES
                     YFPQVDIANFGGGFRVARMNDEENADIKALGKATKKALEDYAKKTGKKLHTEVEPGTF
                     IMANAGNLVTRVMDIKRTGEDGFTFALLDGGMEVLTRPLLYGSEHPFAILNKDGKIRY
                     SDIHSKPSDAQDEIVLVGRCCESGDSFTLNEDHSISPRKMMEPKIDEYLIVGGAGAYA
                     SSMSPFNYNSHQQIAEFLQTKDGKIDCIRKRQSLEQLIENEV"
BASE COUNT         1982 a         1234 c         1256 g         1690 t
ORIGIN      
        1 tgaagaaatt attgcgctta aagatatcac gcttctcgtt gaacctcgat ttaaaacaaa
       61 aaacctaggg attgctttta gcttgttcaa tattccgccg caggttgcgc aaaaactctt
      121 ttatatagaa aatcccctcg gtgtaaatgt ttcacttttc tacaatacct ttgcaaacct
      181 tggttttcaa ggcgaaatcg gtgtgcacac gaccttttct gctcctgaat caacatttgc
      241 actctcacag gatgacttag caatacaaat tgctccgtat ataaaagctg atatcggagg
      301 tggttctctc agtgctgcta ttaaatgcaa ggttctcaac tttacaagcc ttgatggagc
      361 tatagaaaat acgagtcttt caatagggta taaagcgcaa ttatgataaa aaaaaagatt
      421 ttcatttgta taatcttctc ttttattagc tttttaccgg tattttcact agattttggt
      481 tttacaagtc attttctcga tttttcctct gcacaaaacg agccttcttt tgctctatca
      541 tttgacatca cggaagaaat cgcttcaggc atagagattg gagcaaaaat cttgcaatct
      601 aataagcata actacaccgc tttttccaca gcagattttg cattttcatt tttccacctc
      661 ggcggtggta tcatgtacga tattcgtgat gccctgattt cacctggttt tatcgtggat
      721 gcacgagtag atctaaaaaa agaaagcttt ttaggtcttt catactatgc aagtttttca
      781 ccagaaaatc tcgcagaaaa tattagtcaa aatggaaaaa tctacctctc cagcaaaaat
      841 acaaatgcct cactcgaagc ctttgctgac ataacatacg tacaaataca agaagtaaaa
      901 cacaacctca ttttgacatc aaagaccgaa ataatgggtt ttgatgaaaa gacttttatt
      961 gctgtcggct tgcttttttc gtctgagctt tctataaaca cagatatgct tttggaactc
     1021 gatcttgcct ttcgtctcgg tggaaaaatt gcatttaatt ttaaaaaatg gggtgattat
     1081 tttttcacct atgaaagtaa tcttttccac ttgagcgaga aaatacaaaa cataccttct
     1141 tttaatattc actgtggtgc gcgctttagg atcgataatt aagcgatgag taggcattgg
     1201 caaacggagg cccttgtttt acaagtaaaa tccatggggg aagataatcg aagtgttgcg
     1261 cttttaactc gcagcagagg gcttatctgg gctaccttat atggcggtcc aaaaagtaag
     1321 atgcgtgctc tcgtctcgcc ctttcattgt gggcaaatat atctatatac cgatgaagta
     1381 aagcaagcga cgaaaatatc cgattttgca attcactcat atcgcccaga gatacgtgaa
     1441 aatcttttta aaacctgtgc tgcaaatctc tgctcagaac ttgtgataaa aacccatggc
     1501 gggggagaga tagaacagct atggctctat gtaaattctt ttttagatgg gctttgcctc
     1561 gtaaacgagg aagctgctcg ccatgccctc ttacgtttta tctggcgctt tttgaacacc
     1621 atgggtatgc aacctgaccc ttgcgaatgt gcatcttgtg gtacctccgt atgttcgatt
     1681 tacaatacgc aaaaagtctt ttttagctta gcggaatctt cgttttattg tgcgcactgt
     1741 tcttctctgg gctcagagtc taaaggattt tttttacaca aggaagttgt cgactattta
     1801 cgtattatta caacggaagc tcctcgagtt agccgagata ttgtccttga agaagcattt
     1861 ttttatgaaa cccaagattt tttagtctat attttacaaa actttagcgg ccaaaagttt
     1921 aatagtttag atgtcttgtt aaaagtctaa taattctgta tattgttcaa gtagaaaatc
     1981 acattttttg cacaaaccat cttagcaaaa ttatggaggc ttatgcgaat tgatcaagcg
     2041 agtccgtgga caaaaaatta gattagaagg ttttaacaat cttactaaat cattgagttt
     2101 taatatttat gatgtgtcct atgcacattc cgaaaagtca caaattgact atctagagta
     2161 tatcgatgaa gaatacgatt caaaacgttt gcagacgatt attgaagaag taacacgtat
     2221 tatcgatgca aagttggtaa atatatccac acaggattat gacccgcgag gcgcaagtgt
     2281 tgttgccttg ataaacgaag atcatcccgc agaatatgtc aaggaaagcc tcgtttgtgg
     2341 gcaccttgat aaaagccata tcacagcgca tacctatcct gaatacaatt ctcgaactgg
     2401 tttagcgaca tttcgtgtcg acatagatgt gtcaacctgt ggtatgatta gtccccttcg
     2461 tgccctgcac tatcttatcg atagttttga ttccgacata gtactcatgg attatcgggt
     2521 tcgcggtttt acacgagata cacgcggaaa caagattttt attgaccaag atatcagctc
     2581 tattcaagac tatatcgatg aagatgtgct tgctgattat ctagcctacg atataaatat
     2641 tttatcggat acgatatttc atacaaagat gcggaaaaaa aatctcaatc tcgacgacta
     2701 tctttttggt gatgaaatac atgagctaac agaaacggag cgttctcgcg taaaaaagat
     2761 gctcacaaag gaaatacagg agatttttga atgcgcaaat ttataagatt attttcaaaa
     2821 aaacgcacga tgcactttga acatacgagt tctggcggag gttggttttt tgatgtagaa
     2881 aaaaaattgc aatctgcaaa aacccaatgg caaaaaatag atgtggtaaa aaccaaggag
     2941 tttggcaagg cgctcttact cgacggaata acacagttaa ccgaggcaca agagtatcag
     3001 taccacgagc cgatggcgca tgtaccgctt ttggcgcata aaaaccccaa aaaagccctt
     3061 gttatcggtg ggggagatgg cgcccttgca caagaaattt tgcggcatcc gagcattgaa
     3121 agtctcgact ttgttgaact tgacgaaggc gttatcaatt tttgtaaaaa acatttacca
     3181 gagctgggag gagatgcctt taatgacgag cgtgtaaagc tccatattca agacggtaga
     3241 gcctttgttg aaaacgcaga aaaagaaggg aaaacctacg acgcaatttt tatggatatg
     3301 actgatccag aaggtccctg cattgcctta tacacaaagg agttttttga aatagtatct
     3361 agtcttctaa aagatgatga agcttttttt ataatgcacg ccgagtctcc agatttacga
     3421 ctaaaaacct ttcaaagaat aatgcatacg ctcaaggcgg tttttcctag cgtacaagaa
     3481 atggtgagtc atgtgcgaat gtacggcgga atgtggacat gggcgattgc ttctaagggt
     3541 tttaatccca aaaatttcga tgcaaaaaaa atccctgcag ttatagaaaa acgcggcctt
     3601 aaaaacctca aaattatatc gagtgaaaca tgggagagtt tttttaggct ctggcctgtg
     3661 tataaggctc ttttagatga agaaggcgag atagctaccg atgcaaatcc tggctatgct
     3721 atggaataaa aaaggaaaaa aaaatggcaa aaataaaact tgcggtcatt ggctatggaa
     3781 atattggtcg tggtgtttta gaagcaatta aaaaaaactc agatatggag cttgcctgta
     3841 ttctctctcg atctcctgat cgtgttagag ccgaaatagg agggagctac cctatctatg
     3901 cttccgatga ttttgaaaaa ctgcaagaag ccgatgttgc tatactttgc gggggctcaa
     3961 aatctgatct tccagaacaa ggaccagtct atgcccaata cattcacacc cttgacagtt
     4021 acgataatca cagcgaagca tatgcgtatt atcaaacaat ggatgaggca gcaaaaaaga
     4081 cagagcatct ttcttgcata tccataggct gggacccagg aaccttttct gtgcagcgag
     4141 tgctcggcaa tgcctttatt ccaggagcca aaacttatgg cttttatggc acaagtgaaa
     4201 aaggcgggct ttctatggga cactccgatg cactccgacg aatagcagga gtcctcgatg
     4261 ctcggcaata cacgcatgcg atttcagagt ctatcgaaaa agtgcgacga ggcgaaaacc
     4321 cgagtctcaa gccaggcgac atgcactgga gagaatgctt tgttgttgca aaagaaggcg
     4381 ccgatacggc aaaaatagaa gaagcaataa aaacaatgcc tggctatttt gcaggctaca
     4441 aaaccctcgt gcattttgtt tcaaaagaag agctcgaaac aaagcaccaa ggatttcctc
     4501 atgacggcct tgttatagga gcgggaacaa ctggaagtgg aaatcctgca cgcattgagt
     4561 atacaaacat ctggggaagc aactcggaag caaccgcatc catactcgtc gcacatgcac
     4621 gcgcagtcta ccgcctcgca aaagaaggaa agacgggagc aattaccgca ctcgacatag
     4681 cccctgcata tctttcacca cagagtcgag aagatttaat taaaaacttc ttatagaatt
     4741 cttatagaaa tagaggagca tagcaatgaa caagataaat aaaagcgcag tgagtccctc
     4801 tgctattttg gatgcagcaa aaaaaacagc cacacccttt tacatctatg acgaggcatc
     4861 tatacgagag cgatgccaag atatgctcgc tatgcccaat gcctttggac ttacagtgcg
     4921 ctatgcgcta aaggcaaatt ccacaaagcg cctcattcaa ataatcgata aagaaggact
     4981 ccattttgac gcctcctcgc taaacgaagt aaaacgcgca atacaggcag gaataaagcc
     5041 tgaaaaaata ctacttacaa ctcaagaagt ggcaacagat gctgaacttt tggatataca
     5101 agcattgctt aaaaaaggct ttcagtacaa tgcctgctct ttgacacagc taaaaaaaat
     5161 agctcccttt gcttcaaaag aaaatatttc tatagggctt agaattcatc caggagttgg
     5221 atcgggagaa tctgttacaa gaaatacagg agataaatac tcgagttttg gaatccatct
     5281 ttcaaatatc gatgaagcga aggctattat aaaagaatat ggcctagata taaaaagaat
     5341 tcacgtacat attggatctg gtggagaccc agaagcctgg aaggaaaata ttttccgaga
     5401 actctttctt atagaaagtt attttccaca agttgatata gcaaactttg gcggaggctt
     5461 tcgcgttgcc cgcatgaatg atgaagaaaa tgccgacata aaagccttag gaaaagcaac
     5521 aaaaaaagct cttgaagact acgcgaaaaa aacaggcaaa aaacttcata ccgaggtaga
     5581 accaggcacc ttcattatgg caaatgctgg aaaccttgtc acacgcgtca tggatataaa
     5641 acgcactgga gaagacggct ttacctttgc cctcctcgac ggaggcatgg aagtcctcac
     5701 tcgcccactc ctttacggct cagaacatcc ctttgcaatt ctcaacaaag atgggaaaat
     5761 acgctacagc gatattcact caaaaccaag cgatgctcaa gatgaaatag tgcttgttgg
     5821 acgatgctgc gaaagtggcg actcctttac cctaaacgaa gaccactcta tatccccccg
     5881 aaaaatgatg gagccaaaga tagacgaata tctcatcgtt ggaggagccg gagcctacgc
     5941 ctcaagcatg agcccattta attataactc ccatcaacaa attgccgagt ttttgcaaac
     6001 gaaggatgga aagatagact gtatacgaaa aagacaaagc ctcgaacaat tgatagaaaa
     6061 tgaagtgtag aagtgaagta acaagattct tacgtttctt agggaaagag aaccatctta
     6121 cattttttct aacatccgtg ttagaaaaaa cttcgacagt tt
//
LOCUS       DAOSSW010000092         5863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_12452_length_5863_cov_6.567837, whole genome shotgun sequence.
ACCESSION   DAOSSW010000092 DAOSSW010000000
VERSION     DAOSSW010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12452_length_5863_cov_6.567837"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1070)
                     /locus_tag="PKW26_07290"
     CDS             complement(81..1070)
                     /locus_tag="PKW26_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOQ93407.1"
                     /translation="MDTKKLQKLLLILIFLLSIGPIIVFLQGFQEEKKILLLSPKILE
                     TADNIVITENTGSKKESLSFIKKDFWYLRAGERQNIEVPVSKQFTEFLRILSEEKKVR
                     LISTKIKDWEKYRLRDFEADTIEIKNNTSNLAKLYFGMQNAANEIYFRGEKSSVYRGK
                     NPVLFFTKEKTLESQLTSWTDTKWLPSYLGLSRNSIQRLSIEIDNNLKFNTEEQKNKT
                     RILIDKNADTFSSILERLLGSSGALIIDSLEINYLHEKSPLVDIRIENEDTKEYRIKI
                     FPFDRAENKTLQANAEKEKGEKTYLVIPPEANYALEVSRWSIEQLGLEFKSFR"
     gene            complement(1073..3229)
                     /locus_tag="PKW26_07295"
     CDS             complement(1073..3229)
                     /locus_tag="PKW26_07295"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HOQ93408.1"
                     /translation="MSRFFSRALFFKECFQLMRKFSFYLIALFFLCIPFFYYFIYSDF
                     FSFSKLIPINEFFLIFPFIYIILIPFLSMLSWANEGTFLEWLPFSSTSIFFSKWLSSS
                     LSLALFLLSTLVIPFFASFFIYFDWAIIFTSYAFLFVYGIYLISLSQALFVLVNNKLS
                     AFILLMLIIVFGNRFIDSPWAFKGIIQSFEFSSLLLLTFLFFIASVFIKERKKGKKLS
                     RKIFLLIFLVFILALINLNIFSFKIDLTKNKAFSLSNETRQILASLEDDIRIEYYLSR
                     SLEKQYPQVKEVKDFLKLLEKNTNTFRKKNIGLSIINPEKLSEEEIRSLDLKSQQIQI
                     NTKGKTLYEKVFSSIRLQYLDKNLSIDFALSLNGLEYALFSRIQNLIGENEKKVYLLL
                     ANDHALENYSFFLSLLIEEGFIPIDVETHGIDRLDETIPLILIGSNENINDKTPYIES
                     FIMRGGSILFFLSKTKIAFDSDWTGKTKNQDALLEMLDYWGFEIQDGLLIDLENKAEL
                     AMQNLEDDGIETIDYALWQKIEKNNIENPLIDAVKSLDFFWPSPMKIQSSENRKIQAL
                     FLTSEHSLVQDADEEGFFVTDPFALASYRYENQLREQFVLAALGEGSFPGYYSSGKSK
                     PTHLIVVPDEVCISNAMGNIDFLTFVTNSLLYVTGDESLIGLKNKTQAESYFVADIER
                     MPGKTRLEKTASFYAVLGLFPFAVIGLGIFIYRKKR"
     gene            complement(3226..3993)
                     /locus_tag="PKW26_07300"
     CDS             complement(3226..3993)
                     /locus_tag="PKW26_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ93409.1"
                     /translation="MYAIKCTEVSKEYRSFFSKPCLALDNFSAQIHEKKITALLGANG
                     AGKSSLMRLIAAIEYPSSGFIEVNGYSVLEHSLEVKKQIGFMSESADFYPELGVHEFL
                     MLGKKLRKLPQENIDWAIDICALEEVLSKKIQTLSKGYTQRLSLAQALLHKPSILILD
                     EPSSGLDPAQSITFKKLIKNLHTTTLFSSHSTSEIEEIADDVLVLNRGTLVFSGSIES
                     LLEKTDETKLEKAYIRLLSETALEEAKGFENEKGVGL"
     gene            complement(3998..4924)
                     /locus_tag="PKW26_07305"
     CDS             complement(3998..4924)
                     /locus_tag="PKW26_07305"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOQ93410.1"
                     /translation="MNLEAFVLGCGGMMPLPYRALTSVLVRREGDLFLFDGGEGTQVS
                     LRRLNLKWKKINAIFVSHTHADHVTGIPGLMMLSSQVDREEPLYIYGPPIIAEYIGTS
                     RKVLNMYINYPIIVKEITAPCVVHEGDGFYIRAFPLNHTRTCVGYTLEELDRPGEFNP
                     EKAKALDVPCGPLWSKLQSGEDVLSSTGALVKSSDVVGEKRSGRKISFVTDTMYIDSI
                     AKEVRGSDLLFCEGMFDERYADQAKEKKHMTAKQAAKIAKDAKVGKLALIHYSPRYTD
                     YELKTLLDEAQSIFPNTVLSKDRMVFPVPYVD"
     gene            5013..5801
                     /locus_tag="PKW26_07310"
     CDS             5013..5801
                     /locus_tag="PKW26_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93411.1"
                     /translation="MKNTLLLVDPQNDFCEPSGALFVPGAVEDCARLSSFLQKNKAHF
                     SDILVTLDLHPNFHIAHPVFWIDKNGKNPEPYTTITYKHFEEGLYRPAVSSLELQVEK
                     YLLTLENKARYNLTVWPPHCLMATWGACVQKDIYEAISLWEKHHLGKNAEYINKAPNP
                     YTEHYSAVQAEVPIPSDPSTRTNFTFIDYLKKADQVYVAGEALSHCVANTLRDLCVYI
                     PAKKVTVLTDCTSPVKGFEDVGLAFVDEYSAKGMLFKTSTEIQF"
BASE COUNT         1763 a         1062 c         1115 g         1923 t
ORIGIN      
        1 tcctacttct actcatgttt ttgtttattt gaaaagatag atttccagtt tcccaacttg
       61 tcttacttgt gaaaagcaca ttatcggaag gatttaaatt ctaagcccag ttgttctatg
      121 gaccatcggc ttacttctaa agcgtagttt gcctctggtg gaattacaag ataggttttt
      181 tcaccttttt ctttttcagc attagcttgc agagttttat tttctgctct atcgaagggg
      241 aatattttta tgcgatactc ttttgtatct tcgttttcta ttctgatatc tacgagagga
      301 gatttttcat gtagataatt gatttcaagg gaatcgatta tcagggcacc agagcttccc
      361 aaaagacgct cgaggatgct gctaaaagta tctgcatttt tatcgattag gattcttgtt
      421 ttattttttt gctcctctgt gttaaatttt agattattat caatttctat ggacagccgc
      481 tgaatggaat ttcttgatag tccaagatag ctaggtaacc attttgtatc ggtccatgaa
      541 gtgagctggc tttcgagtgt tttttccttt gtaaaaaata aaacaggatt ttttcctcga
      601 tagacagaag atttttctcc acgaaaatat atctcgttgg cagcattttg cattccaaaa
      661 tagagttttg ctagattgct tgtattgttt tttatttcaa ttgtatctgc ttcaaaatcg
      721 cgcagccggt atttttccca gtcttttatt tttgttgaaa taagccgaac ttttttttct
      781 tcactcaaga ttcttaaaaa ttctgtaaat tgcttactta ctggtacttc gatattttgc
      841 ctctcccctg ccctcagata ccaaaaatct ttttttataa aactgagact ttctttttta
      901 gagccagtat tttctgtgat gacaatattg tccgctgtct caagaatttt ggggctaaga
      961 agcaggattt ttttttcttc ttgaaaccct tgtaaaaaaa cgatgatagg tcctatcgat
     1021 aaaagaaaaa taagtatgag taatagtttt tgtagttttt tagtatccat atctatctct
     1081 tttttctgta gataaaaatg ccaagcccga taactgcaaa tggaaaaagt ccaaggactg
     1141 cataaaaaga ggctgttttt tctaaacgtg tttttcctgg catcctttct atatccgcga
     1201 caaaatatga ttctgcttgc gttttatttt ttaagcctat gagcgactca tctcctgtaa
     1261 catagaggag actattggta acaaaggtga gaaaatcgat atttcccata gcattcgaaa
     1321 tacaaacttc gtcgggaaca actatgagat gtgttggctt cgattttcca ctactataat
     1381 agccggggaa gctgccttca ccgagagctg ccaagacaaa ttgctctcgt agttgatttt
     1441 cataacgata ggatgcgagg gcgaaggggt cggttacaaa aaaaccttct tcatcggcat
     1501 cttgtacaag gctatgttca cttgttagaa aaagagcttg aatttttcgg ttttcgctcg
     1561 attggatttt catgggactt ggccaaaaaa agtccaaact tttgacagca tctatgagag
     1621 gattttcgat attgtttttt tctatttttt gccagagggc gtagtctatt gtttcaatcc
     1681 catcgtcctc tagattttgc atagctaatt ctgccttatt ttccaagtcg atgagaagtc
     1741 cgtcttgtat ttcaaaaccc cagtagtcga gcatttctaa gagcgcgtcc tgattttttg
     1801 ttttgccggt ccagtcacta tcgaaggcga tttttgtctt tgacagaaaa aagaggattg
     1861 agcctcctcg cataataaat gattctatat acggcgtttt atcgtttata ttttcattag
     1921 atccgataag aatcaatgga atagtttcat cgagcctatc tatcccatga gtttctacat
     1981 cgatgggaat aaaaccttct tcaatgagca aactgagaaa aaatgaatag ttttccaatg
     2041 cgtgatcatt tgcaagaagg agatagactt ttttttcatt ttcaccaatg aggttttgta
     2101 tacgcgaaaa aagagcgtat tctaaaccat ttaagctaag tgcaaagtca atgcttaagt
     2161 ttttatcaag gtattggaga cgaatactcg aaaaaacttt ttcataaagc gttttaccct
     2221 ttgtgtttat ttgtatttgt tggctcttta aatcaagcga gcgtatttct tcttcgctga
     2281 gtttctctgg atttataata gaaagcccta tgtttttttt gcgaaaggta tttgtgtttt
     2341 tttccaaaag tttcaaaaag tcttttactt cttttacctg tggatattgt ttttccaaac
     2401 tgcgcgaaag ataatattcg atgcgtatat cgtcttcaag acttgcaagt atttgccttg
     2461 tttcgtttga taagctaaat gccttgtttt ttgtgagatc gattttaaaa gaaaaaatat
     2521 ttagattgat aagggcaaga ataaaaacaa gaaagatgag tagaaagatt ttccgagaaa
     2581 gtttttttcc tttttttctt tctttaataa aaacacttgc tataaaaaaa agaaaagtca
     2641 aaagaaggag gcttgaaaat tcaaagcttt gtattattcc cttaaatgcc cagggagaat
     2701 cgataaaacg gtttccaaaa acaatgatga gcatgagaag tataaaggcg ctgagtttgt
     2761 tattgactaa aacaaagaga gcttgagaca aggatatgag gtagattcca taaacaaaaa
     2821 gaaatgcgta gctcgtaaaa ataattgccc aatcgaagta gataaaaaac gaggcgaaaa
     2881 aaggtatgac gagtgtggat agaagaaaga gagcaaggga gagtgaagag ctaagccatt
     2941 ttgagaaaaa tatactcgtg ctagaaaaag gaagccattc taaaaaagtg ccctcatttg
     3001 cccatgagag catgcttaaa aaaggaatta aaataatata gatgaaggga aaaataagaa
     3061 aaaattcgtt tatgggaata agttttgaaa aactaaaaaa gtcgctatat ataaaataat
     3121 aaaaaaaggg gatacaaaga aaaaaaagag cgataagata aaaactaaac ttacgcatga
     3181 gttgaaaaca ttctttgaaa aacaatgctc ttgaaaaaaa acgactcata gccctactcc
     3241 tttttcgttc tcgaagcctt ttgcttcttc taaggcggtt tctgaaagaa gtcgtatata
     3301 ggctttttca agttttgttt catcggtttt ttctaagaga ctttcaatgc tccctgaaaa
     3361 aactaaggtt ccgcgattta aaactaagac atcgtcggca atttcttcta tttcgctggt
     3421 gctatgcgag gaaaaaagcg tcgttgtgtg tagatttttt ataagctttt taaaggtgat
     3481 tgattgggcg gggtctaaac ctgagctcgg ctcatcaagt atgagaatgc ttggtttatg
     3541 taaaagggct tgtgcaagag agagcctttg tgtatagccc tttgaaagtg tttgtatttt
     3601 tttggagagt acttcttcga gagcacatat gtctattgcc caatcgatat tttcttgtgg
     3661 aagtttacga agcttttttc cgagcataag aaattcatgc actccgagct cagggtaaaa
     3721 atctgcagac tcgctcataa agccaatttg tttttttacc tcaagactat gctcaagaac
     3781 agaatatcca ttcacttcta taaaacctga gctcggatat tcgatggcag cgatgagacg
     3841 cataaggctt gattttcctg caccatttgc tccaagcaag gcggtgattt ttttttcgtg
     3901 tatttgtgca gaaaaattat ccaaggcaag gcaaggtttt gaaaaaaaac tgcgatattc
     3961 tttgcttact tccgtacatt ttattgcata cattttttta atctacatat ggaacaggaa
     4021 ataccattct gtctttggaa agtacggtat ttggaaagat tgattgcgct tcatcgagta
     4081 gtgtttttaa ttcatagtca gtatagcgcg ggctataatg tatgagggca agttttccga
     4141 cctttgcatc ctttgctatt tttgcagctt gctttgcggt catgtgtttt ttttctttcg
     4201 cttgatccgc atagcgctcg tcaaacattc cttcgcagaa taaaaggtca gaccccctca
     4261 cctcttttgc aatgctatct atgtacatag tgtcggtaac aaagcttatc tttcggccac
     4321 ttcttttttc acctacaaca tccgagcttt ttacaagtgc ccctgtgcta gacagcacat
     4381 cttcgcctga ttgcagtttc gaccataagg ggccacaggg aacatcgagt gcctttgcct
     4441 tttctggatt aaactcacca ggtctatcca actcttcgag cgtatagcca acacaggttc
     4501 gggtatgatt gaggggaaaa gcgcgaatat aaaagccatc cccttcatgc acaacgcagg
     4561 gtgcggtgat ttcttttacg ataatgggat aattgatata catattgagt acttttcgac
     4621 ttgttcctat gtactctgca attatgggag gtccataaat atataagggc tcttcacggt
     4681 ctacttggga tgaaagcatc attaaaccag gaatacctgt tacgtggtca gcatgggtat
     4741 gacttacaaa aatggcattt atttttttcc atttgaggtt tagtcttcgc aaggagactt
     4801 gagttccctc acccccatca aataaaaata agtccccttc tcttctaact aaaactgagg
     4861 ttagggctct atatggaaga ggcatcatgc ccccacaacc taatacaaac gcttcaagat
     4921 tcatgccata actataccga aaaatagcca aatatgcaac tcgtagcata gccagtattg
     4981 acacagaggt atgcttaaag tattataaat acatgaaaaa taccttgctt ttagttgatc
     5041 cgcaaaatga tttttgcgaa ccatcaggtg ccttgtttgt accaggcgca gttgaggatt
     5101 gtgcacggct ttcatctttt ttacagaaaa ataaagcgca tttttcggat attttagtta
     5161 ccttggattt acatccaaat tttcacattg cacatccagt attttggatc gataaaaacg
     5221 gaaaaaatcc agagccctac acaacaatta catacaagca ttttgaagaa ggactatatc
     5281 gacctgcggt ttcgtccttg gaactacaag ttgagaaata cctactcacc cttgaaaaca
     5341 aagcacgcta caaccttact gtatggccac cgcattgtct tatggcaacc tggggagcat
     5401 gtgtacaaaa agatatttat gaggctattt cgctttggga aaaacatcat ttaggaaaaa
     5461 atgccgagta tattaacaag gcgccaaatc cttataccga gcactactct gctgttcaag
     5521 cagaagtacc catcccctca gaccccagca cgcgaaccaa cttcaccttt atagattact
     5581 taaaaaaagc tgatcaggta tatgttgcag gagaagcact ttctcactgt gttgcaaata
     5641 cgcttcgaga cctttgtgta tatatacctg caaaaaaagt aaccgtttta acagactgca
     5701 cttcacctgt aaaaggtttt gaggatgttg gcttagcctt tgttgatgag tatagcgcaa
     5761 aaggaatgct ttttaagact tctaccgaga ttcagttcta ggggattttt ttgccgaaca
     5821 ataacaaaat aaggtaaata ctaagaagct ttttttacaa aga
//
LOCUS       DAOSSW010000093         5809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_12592_length_5809_cov_8.379910, whole genome shotgun sequence.
ACCESSION   DAOSSW010000093 DAOSSW010000000
VERSION     DAOSSW010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12592_length_5809_cov_8.379910"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..134)
                     /locus_tag="PKW26_07315"
     tRNA            complement(62..134)
                     /locus_tag="PKW26_07315"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(99..101),aa:Met,seq:cat)
     gene            293..1240
                     /gene="argF"
                     /locus_tag="PKW26_07320"
     CDS             293..1240
                     /gene="argF"
                     /locus_tag="PKW26_07320"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOQ93412.1"
                     /translation="MIQDTKNCLQGRSLTNFIDYSADEIRYLIELALKVKKQAQNKEI
                     HQRFIGKSLALLFEKRSTRTRCAFETAFGEEGGHPVFLSSHDIQLGAKESVEDTARVL
                     GRMFSGIQFRGFSQEHVETLAKYAGVPVYNGLTDSFHPTQVLADMMTLTEIYGDLKGL
                     HLAFCGDGRNNVARSLMLISAKLGLDFTIVSPKEFFPDSEILSICTPIAKKNNANITI
                     SDKISLVKGADSIYTDVWISMGEEALSTEKKALLQSYQVNKDLMALTGKKDSVFMHCL
                     PAVKGEEVTQDIIEGSQSRVWDQAENRKHTIKAILLATL"
     gene            1292..1702
                     /locus_tag="PKW26_07325"
     CDS             1292..1702
                     /locus_tag="PKW26_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93413.1"
                     /translation="MKKKFVLLSLLVLLSLALYAQSASETEENFTYFNVDIIKVYQHK
                     DAYFVLYNKSGNKMGQVALPKEWFQYGSGNHSRIRPLPSKLAPYLTVIYKDNEFYKVY
                     LNMPVDRLHPAWAVLDNNIDITARINKETLDISF"
     gene            1771..2751
                     /locus_tag="PKW26_07330"
     CDS             1771..2751
                     /locus_tag="PKW26_07330"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOQ93414.1"
                     /translation="MNYLNEAAIQSFKTFIDSHDAFYIAGHKEPDGDSISSSLGLAEI
                     LTLCNKPFQLLCAGPFKRTEIKKYEKQFLKTPKPFTHDRKKIGFFIVDCAEYERVGDF
                     ANELQDLDSFIIDHHRTSDAIKNGILDIEAPATSIIIQLLYEHFFKTMSKKIAHIFLF
                     GICTDTGFFRFLDEKSSLVFEAVSRLIKYGASPKLIYEKMTGGKPFETRKLLSILLDR
                     AERTFNGQLVYTFETLEDTKKYGKKGRDSDALYQVFMAVDKVKAVFVVRQDTDKTCTA
                     GFRSKDDLDVSIVAAKFGGGGHKNASGASYTGNIRDFIEKILIEMSKIIS"
     gene            2878..3723
                     /locus_tag="PKW26_07335"
     CDS             2878..3723
                     /locus_tag="PKW26_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93415.1"
                     /translation="MQIKNTNAIFNSFLKGKISKKEAVNAILVYLFQNKLYFGLETMG
                     EDDFNDFLIYLIPIIGKSFENYSKLECSFATYFQSIVRRNKKNFHRNLLLKKSKQNFI
                     YHVSKLDYFDNLEKNDCENLRVEEEIDTKQSFSNKQLLIIALKCAYFLDDDKIKKIAE
                     KTGYHERYIWNLKKKIDTEMASRYEKNKKRIEAINKRYYLKHKANIYARLQSKEDAVY
                     ERAQYLKKFHSMRWEKISHKTNLRFIVPTNQFLSEILGLKETVVANAIVRSRKYVQNM
                     ETTKN"
     gene            3804..4400
                     /locus_tag="PKW26_07340"
     CDS             3804..4400
                     /locus_tag="PKW26_07340"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HOQ93416.1"
                     /translation="MEVYLASENEDKKKEIQAYFPEWKILSPKDTAISFNPIENGSSF
                     LENALIKAKLLWNLVQKPVIADDSGICLSDLDNRPGIHSARYRGKTQAEKNKNLIDEL
                     NALAKTKTNPVDRHCFYVCSLVYYYGENRYFSVQETLEGELIRDISEQRGCGGFGYDP
                     IVYLPQLNKMVAELSSEEKNAISHRGKALSALKVFLSK"
     gene            4588..5448
                     /locus_tag="PKW26_07345"
     CDS             4588..5448
                     /locus_tag="PKW26_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024752187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOQ93417.1"
                     /translation="MVINHNLSAMYSNRSLGVTNLSTTKDMEKLSSGMKINRAGDDAS
                     GLAVSEKMRSQIRGLNQASQNASNAISFIQATEGYLQNTTDIVQRLRELAVQSSNGIY
                     SSEDRMQIQVEVSQLVAEVDRIANSAQFNNMNMLTGRFAEQTGENVVTASMWFHVGAN
                     MDQRMNAFIGTMTASALGLRSIGSEEILSLAAPDDANASIGVLDEALKKINKQRADLG
                     AYQNRLDFTVKGIDIAAENLQAAESRIRDTDMASQMVEFTKNQILTQAGTAMLAQANA
                     RSQSVLSLLQ"
BASE COUNT         2051 a         1035 c         1019 g         1704 t
ORIGIN      
        1 catttttgat ttttataaag taaagaaaaa tgactgctct caatgaacag tcatttttct
       61 atagcggcaa tcgggattga accaatgacc gaacggatat gagccgtttg ctctgccaac
      121 tgagctatgc cgccatgttt ttttactata ctaaatttag aaaattgtgt caactcaacc
      181 aaagcagata gatacagaaa agctcatata aaaatttatc acgatactga gaaaaaattt
      241 attgactaag ttttctctct ataatacaat aaaatttcaa taggaaaata aaatgataca
      301 agatacaaaa aactgtttac aaggacgctc cttgactaat tttatcgatt attcagcaga
      361 tgaaattcgc tatctgatag aactcgcact caaggtaaaa aaacaagcac aaaataaaga
      421 aattcaccaa cgatttatag gcaaaagtct tgccctcctc tttgaaaaac gctcaacacg
      481 aactcgttgt gcttttgaaa cagcctttgg cgaagaaggt ggacatcctg tctttctttc
      541 ctcccatgat atccaacttg gtgcaaaaga atcggttgaa gataccgccc gagttctcgg
      601 aagaatgttt tctggtattc aatttcgtgg cttttctcaa gagcatgttg aaacacttgc
      661 aaaatatgca ggcgttcctg tgtacaatgg cttaacagat tcctttcatc ccacacaggt
      721 tcttgcagat atgatgaccc tcactgaaat ctacggcgat ctcaaaggct tacatcttgc
      781 cttttgtggt gatggaagga ataatgtagc gcgctccctc atgcttatct ctgcaaaact
      841 cggtctagac tttacgattg tgtcgccgaa agaatttttc cccgattctg agattctttc
      901 tatctgcaca cctatcgcca agaaaaacaa tgctaatatt actatttctg ataaaatttc
      961 gcttgtaaaa ggagcagatt cgatttatac tgatgtatgg atatctatgg gcgaagaagc
     1021 actttctaca gaaaaaaaag cccttcttca atcatatcaa gtaaacaagg accttatggc
     1081 tttaacaggg aaaaaagaca gtgtttttat gcactgccta ccagctgtta aaggcgaaga
     1141 agttactcaa gatatcatag aaggatcaca aagtcgtgtt tgggatcaag cagaaaacag
     1201 gaagcataca ataaaagcaa tactacttgc aacattatag atttcaaatt ctgctattat
     1261 ttgctcttat tcagtaacac atggaggttt tatgaaaaag aagtttgttt tattgagtct
     1321 tttggttttg ctgagtcttg ccctttatgc acaaagcgca agtgaaaccg aagaaaattt
     1381 tacgtatttt aatgttgata ttataaaagt ctatcaacat aaggacgcat acttcgtctt
     1441 atacaataaa agtggaaata aaatggggca agtagcctta cctaaagaat ggtttcagta
     1501 tggaagcgga aaccatagtc gtatacgtcc cctaccaagt aaactagctc cgtaccttac
     1561 tgttatctac aaagataacg aattttataa ggtgtattta aacatgcctg ttgacagact
     1621 ccatcctgcc tgggctgttc tagacaataa catagatata acggcgagaa taaacaaaga
     1681 aacacttgac atatcatttt aatgcgcaca tggtgatata acgttgctat atcaccttta
     1741 cactgtccaa ggtgttttta taggaatttc atgaattatt tgaacgaagc cgctattcag
     1801 tcatttaaaa catttataga ctcgcacgat gccttttaca tagctggtca taaagaacct
     1861 gatggcgata gcatatccag ttctctcgga cttgccgaaa tactcacttt atgtaacaag
     1921 ccctttcaac tcctttgcgc aggacctttt aaaagaactg aaataaaaaa atatgaaaaa
     1981 caattcctta aaacaccaaa accctttact catgatcgta aaaagattgg tttcttcatc
     2041 gttgattgcg cggaatacga acgagttgga gattttgcaa acgaactaca agaccttgac
     2101 agctttatta tcgaccatca tcgtacttca gatgcgatca aaaatggtat tttagatatt
     2161 gaagcacccg caacatcaat aattattcaa ctcttatatg aacatttttt taagactatg
     2221 tcaaaaaaaa ttgctcacat ttttctcttt ggcatttgta ccgacaccgg tttttttcgt
     2281 tttttagatg aaaaaagctc tctcgtgttt gaggcagttt cacgcttgat aaaatacgga
     2341 gcaagtccaa aactcatata cgaaaaaatg actgggggaa aaccctttga aacaagaaaa
     2401 ctattgtcaa tcctcttaga tagagctgaa cgaaccttta acgggcaact cgtttatacc
     2461 tttgaaaccc tcgaagatac gaaaaaatac ggaaaaaaag gaagggactc cgatgccctc
     2521 tatcaagttt ttatggcagt cgataaggta aaagccgttt ttgttgtccg acaagatact
     2581 gacaaaacct gtaccgccgg ttttcgttca aaggacgatt tagatgtcag tattgtggca
     2641 gcaaaattcg gaggtggtgg tcacaaaaat gcctcgggtg caagctatac tggaaatatt
     2701 agagatttta tagaaaaaat ccttattgag atgtcaaaaa taatctccta aatctctcat
     2761 accttgaaat tatctattta tttaaaaaaa agaagcctca tttaagctaa atctagggaa
     2821 aaatcttctt taaagttggc acaaaatttg cattatattc tctaaaagga gtaatatatg
     2881 caaattaaaa acacaaatgc tatttttaat tcatttctaa aaggaaagat aagcaaaaaa
     2941 gaggctgtca atgcaattct tgtgtatctt tttcaaaaca agctctactt tggattagaa
     3001 actatgggag aagacgactt caatgatttt ctcatctacc tcatcccaat tataggaaaa
     3061 agctttgaaa actatagtaa gttagaatgc tcttttgcta cgtattttca aagcatagtc
     3121 agacgtaaca aaaaaaactt tcacagaaat ctgctattga agaaaagtaa acaaaatttt
     3181 atttatcatg tttcaaaatt ggattatttt gataatcttg aaaaaaatga ttgtgagaac
     3241 ctgcgtgtag aagaggaaat agacacaaaa cagagttttt caaataaaca acttttaata
     3301 atcgctctta aatgtgcata ttttttggat gacgataaaa taaaaaaaat agcagaaaaa
     3361 actgggtatc acgagcgcta tatttggaac ttaaaaaaga aaatagatac agaaatggca
     3421 agccgctatg aaaaaaacaa aaagcgtatt gaggctataa ataaaagata ttacttaaaa
     3481 cataaggcaa atatctatgc tcggctacaa tcaaaggaag atgcagtata tgaaagagcg
     3541 cagtatctaa aaaaattcca ctccatgcgc tgggaaaaaa taagccataa aacaaattta
     3601 cgatttatcg taccaacaaa tcaattttta tcagaaattt taggtcttaa agaaacagtc
     3661 gttgcaaatg ctatcgtaag atcgcgaaaa tatgtgcaaa atatggaaac cacaaaaaat
     3721 taaaacattt tacaaatcat ttcgtaatca atcgagaaaa aaaggaacta gaattcgcat
     3781 aaaaattttg gtacaattca cagatggaag tctatttagc aagcgaaaat gaagataaaa
     3841 aaaaagaaat acaagcctat ttcccagaat ggaaaatcct ttcaccaaaa gataccgcta
     3901 tttcttttaa ccctatagaa aacggaagta gttttttaga aaatgcgctt ataaaagcaa
     3961 aactgctttg gaatcttgtg caaaaaccag ttattgctga tgattcaggt atttgcctga
     4021 gcgatttgga taatcgccct ggaatacatt ctgctcgata taggggtaaa acgcaggctg
     4081 aaaaaaataa aaatcttatc gacgaattga atgctttggc taaaactaaa acaaatcccg
     4141 tagatcgaca ctgcttttat gtttgctccc tcgtgtatta ttacggagaa aaccgttatt
     4201 tttctgtcca ggaaaccttg gaaggagaac tcattcgaga tatcagcgaa caaagaggct
     4261 gtggcggttt tggctatgac cccatcgtat atcttccaca actcaataaa atggtcgctg
     4321 aacttagctc tgaagaaaaa aatgctattt ctcatagagg aaaggcatta agtgctttaa
     4381 aagtattttt atcaaaataa ttattttttt tactaaagtt ttctactcaa actaccgata
     4441 taaaaatgag ggaaggattt accctttttc gttgaaaaag agcaaggatt tcccatctgt
     4501 gtaattttca cgtatgtagc ctgcaaacgt gcaaaaacac aaaagaaaac aaggatgttt
     4561 tctagctatt tccatggagg aacaattatg gtaatcaacc acaacttgtc agcaatgtat
     4621 tccaatcgat ctttaggtgt tacaaactta agcaccacaa aagatatgga aaaactctca
     4681 tcaggtatga aaattaaccg tgccggtgat gatgcatctg gtttggcagt ctctgaaaaa
     4741 atgagaagcc aaattcgcgg cttgaaccaa gcctctcaaa acgcttcaaa cgcgatcagt
     4801 tttattcagg caactgaagg gtatttacaa aataccactg atatcgtaca gcgattacga
     4861 gagcttgcag tacaatcttc aaacggaatt tattcttcag aagatcgaat gcaaattcaa
     4921 gttgaagtgt cacagcttgt agccgaagtt gatcgaattg caaactctgc acagtttaat
     4981 aacatgaaca tgttaactgg tcgatttgcc gaacaaactg gtgaaaatgt tgtaactgca
     5041 tcaatgtggt tccacgtcgg tgccaacatg gatcaaagaa tgaatgcttt tataggaact
     5101 atgacagcat cagcccttgg tcttagaagt attggttctg aagagatttt aagccttgct
     5161 gctccggatg atgccaatgc atctatcggt gttcttgatg aagctcttaa aaagataaat
     5221 aaacaaagag ctgaccttgg tgcctatcaa aaccgcttag acttcaccgt taagggtatt
     5281 gatattgctg ccgaaaactt gcaagctgct gaatcacgaa ttcgtgacac agatatggct
     5341 tcgcaaatgg tagaattcac caagaatcaa attcttacac aagcaggaac agctatgctt
     5401 gcacaagcta atgctcgttc tcaaagtgtt ttatctttac ttcaataaag aataacgata
     5461 ataaaaaagc actataatgc ttttttatca cttgtataca attatgattt cataattgta
     5521 tacaagctgg ttgcagtcta tatcttcata acttgtttct caatctgcaa tatcaatagt
     5581 atactgataa ttatataaaa ctgcatgttt ttattctttt agtgtggacg tttagttaaa
     5641 gctaaggtat aatactacta gagtaattta aaactgacag tctctgtaaa gtggcgcaaa
     5701 aatactgaga ttgggtttta acataaagcg atggattgct ttatgaaaat catgacatgg
     5761 agggtatgta tgattattaa tcacaacatg agttctatgt atgccaacc
//
LOCUS       DAOSSW010000094         5735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_12783_length_5735_cov_6.986620, whole genome shotgun sequence.
ACCESSION   DAOSSW010000094 DAOSSW010000000
VERSION     DAOSSW010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12783_length_5735_cov_6.986620"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PKW26_07350"
     CDS             <1..918
                     /locus_tag="PKW26_07350"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOQ93418.1"
                     /translation="LIRYFRESREKSFLQKAIGTYVSNEVLNEIIEDPTKLKLGGESR
                     HMSVMFSDVKGFSSISEKLTPEQLVTLLNTYLTGMSNIVLDEKGVVDKYEGDAIIAFW
                     GAPNILENHAYHSIAAAVKMKTLEAELNKTFMEEKKSPVPLLTRFGINTGEMVAGNMG
                     TERKMNYTVMGNAVNLAARLEGVNKMYGTWILTTDYTAKEAGDEFMYRRLDQVRVVGI
                     NKPVQLLEVIDFKNSINSEKESFLKDFEKALDLFNERNYKAAQDIFDNLLKINAEDGP
                     STIYRDRCIDFIKSPPAKDWDGVFNLTQK"
     gene            complement(942..1556)
                     /locus_tag="PKW26_07355"
     CDS             complement(942..1556)
                     /locus_tag="PKW26_07355"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HOQ93419.1"
                     /translation="MRIAKLERNTNETQINIELNLDGKGLCTIESPVGFLNHLLASFC
                     KHGLFDLSGKIEGDLDVDQHHLIEDTGIVLGQAFAKALGNCAGIFRSGSCLYPMDETL
                     ARAAVDFGGRAYLVCEADLTNIPLVSINKNGLNSSFQTDCFEDFWLGFVGGAKCNLHL
                     DTLRGRNDHHKMEALFKAASRAIREAVSIDERRGGDIPSTKGLI"
     gene            complement(1575..2429)
                     /gene="hisG"
                     /locus_tag="PKW26_07360"
     CDS             complement(1575..2429)
                     /gene="hisG"
                     /locus_tag="PKW26_07360"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOQ93420.1"
                     /translation="MEQLKILLPKGRIYDNVVELFKGAGITITLPDRAYRPTVNQDDL
                     EAKVMKPQNIGKLLEFGAHDVGFTGRDWVLETGADVEEIMDLGFDRVRIVAAVPKELG
                     DSILESKRIVVATEYEKIAQSWLEKKGFNYLTVRTYGATEVFPPDDADMIIDNTSTGR
                     TLIENGLRIVDTIMTSATCMFASKEAMKDPFKKKKILELKMLFEAVLDARDRVMLEMN
                     VAKNKFDSLVQGLPSMRSPTVSPLFGDDGFAVKIAVKKSEVPNLLPKLKNLGATDILE
                     YDLRKVLA"
     gene            2688..3443
                     /locus_tag="PKW26_07365"
     CDS             2688..3443
                     /locus_tag="PKW26_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOQ93421.1"
                     /translation="MKQKKSLAVCGYKAVKTLANTNPEKILRFYYSKERSSEFGMLCK
                     KLAENKIPYNQVENNELEKLSKSLHHQGLVAFIAEPEIEQLSKEKVLEFMRNKEKLVF
                     LDLVGNSNNLGAIIRSAAFFGIRHIIIPENEKQASIATSTYRVAQGGMEFVSLYFVKS
                     PEFFLQDIAGKIIRIGTDLEAKKNISDISHIYTPQGEKKASLIILGNEEEGISPQIKN
                     LCDELVKIPGGKNMQSLNVAQAASIIFYELTRR"
     gene            complement(3466..4764)
                     /locus_tag="PKW26_07370"
     CDS             complement(3466..4764)
                     /locus_tag="PKW26_07370"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ93422.1"
                     /translation="MSNIDAYLDIARLITSVCIQIIVVLFSFKLRIKKRYAFLSLIAC
                     LFCNIILDYFLEKRGNSFLLALQLDTFLFCIVIVLISGGSIYGQIFFHYVQNLIANCI
                     FIIGKECLVYGKMAEFLAIEFLMIAYLVLMLSFGKEIISAVRDFLDRPAWSFLLVYPV
                     ASYYAIRLLGFEAEKALYTPNTSLSLALSLLAGNCMAILCIVLVQKKKAVELALLEGK
                     IALSASEAYYQRVSEMLDHVRIIRHDYKHELGIIKQLLGKGEIDTLKKILSVPSAIDE
                     GNAKLYCKNVVVNALLVHYVFICNKHCIPVEIDAMLPQLESEHLAENKKDLTNYDLCI
                     IIGNLFENAIEESIKVEEKDRSISIQIDSDIHKILIKVINTCVAERAKDSKALPITSK
                     KEYAHGLGLKSVKMTCEIFGGTFFWSCENNLFTASAIVNR"
     gene            complement(4778..5557)
                     /locus_tag="PKW26_07375"
     CDS             complement(4778..5557)
                     /locus_tag="PKW26_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOQ93423.1"
                     /translation="MTVLLVDDNSLELENLASFIQEWNTQRTDTAKSENPLRLCICSS
                     AKETLTVLKTLKNEVYPNAAILDIVMPETKGTELAIELRKKNFTGPIVFLTSSNEFAC
                     ESYEVHAFSYLLKPINKKKLFSVLSSIEDHLQQKKERKIDEASILLSSKSWKEKVLFS
                     HLMWVEVKSHYLYFHIKNEEGQKVLKTGGSLSATGEVLLQDERFASCHSSFIVNMDYV
                     QTVANNEAIIHTGEKLPISRRHSEFKSLYVDYVLKKARRYL"
BASE COUNT         1867 a         1164 c          978 g         1726 t
ORIGIN      
        1 ttaattcgct actttagaga atcacgagaa aaatcatttt tgcaaaaggc aattggaacc
       61 tatgtttcaa atgaagttct taatgaaatt attgaagacc caaccaaact taagcttggt
      121 ggtgaaagcc gtcatatgtc tgttatgttt tctgatgtaa aaggtttttc aagcattagt
      181 gaaaaactta caccagaaca gcttgtaact ttgctcaaca cttatcttac gggcatgagt
      241 aatattgttt tagatgaaaa aggtgttgta gataaatatg aaggtgatgc aattattgcg
      301 ttttggggtg caccaaacat tcttgaaaac catgcctatc attccatagc agctgcagta
      361 aaaatgaaaa cgcttgaagc tgaattaaac aaaactttta tggaagaaaa aaaatctcct
      421 gttccgcttt taactcgctt tggaattaat acaggcgaaa tggtcgcagg taacatgggt
      481 actgaacgaa agatgaacta cacggttatg ggaaatgcag taaatcttgc tgctcgctta
      541 gaaggcgtaa ataaaatgta tggaacctgg atattaacga ccgattacac agcaaaagaa
      601 gcaggagatg aatttatgta ccgccgactt gaccaagtac gcgttgttgg tataaacaaa
      661 cctgtacaac ttctagaagt gatagacttt aaaaacagta taaacagtga aaaagagagc
      721 tttctcaagg actttgaaaa agctctagac ttatttaatg aacgaaacta taaggctgca
      781 caagatattt ttgacaatct cttaaaaata aatgcagaag atggaccaag tactatttat
      841 cgagaccgtt gtatagactt tataaaaagc cctcctgcaa aagattggga cggcgttttt
      901 aatttaactc aaaaataata caagaggctt aaacaagctt tttagataag cccctttgta
      961 gaaggaatat cgccgccccg cctttcatcg attgaaacag cttctcgtat agcacgggag
     1021 gctgctttaa aaagcgcttc cattttgtgg tggtcgtttc ttcctcgtag agtatcgagg
     1081 tgaaggttgc actttgcgcc cccgacaaag ccaagccaaa aatcctcaaa gcaatctgtt
     1141 tgaaagctag agttcagacc gtttttattg attgaaacaa ggggaatatt tgttaaatct
     1201 gcctcacaga caagatatgc acgaccacca aaatcgacag cggcacgagc aagcgtttca
     1261 tccatgggat acaagcagct cccgcttcga aaaataccag cacaatttcc caaggccttt
     1321 gcaaaggctt gtcccaaaac aatgccagta tcttctatta aatggtgttg gtctacatct
     1381 aaatcgccct ctatctttcc gctcaaatca aataaaccat gtttacaaaa actcgcgagc
     1441 aagtggttca aaaacccaac aggactttcg attgtacaaa gtcccttccc atcaagattg
     1501 agctctatat tgatctgcgt ttcatttgtg tttcgctcaa gtttagcaat tctcattttt
     1561 tcccctttcg ttttttacgc aagtactttt cgtaaatcat actctaaaat atctgttgca
     1621 cccaagtttt ttaactttgg aagcaagtta ggcacttcgc tttttttcac tgcaatcttt
     1681 accgcaaaac catcatcacc aaaaagaggc gaaacggttg gactacgcat tgaagggagg
     1741 ccttggacca agctatcaaa cttatttttt gcaacattca tttcaagcat aacacggtcg
     1801 cgagcatcta aaacagcttc gaaaagcatc tttagctcta aaatcttctt tttcttaaag
     1861 gggtccttca tcgcctcttt tgaagcaaac atacaagttg cgctcgtcat aatagtgtct
     1921 acgattcgca aaccgttttc gatgagcgtt ctccccgtcg aagtgttatc gataatcata
     1981 tctgcatcat ctggtggaaa aacttccgtc gccccgtaag tacgaacagt aagataattg
     2041 aagccttttt tttcaagcca agattgcgca attttttcat attctgttgc aacaactatc
     2101 cgctttgatt ctaaaatact atcgccgagc tcttttggaa cagctgcaac aatacgtacc
     2161 ctgtcaaaac caagatccat tatctcctcg acatcggcac ccgtttccaa aacccagtca
     2221 cggccagtaa aaccgacatc gtgtgctcca aattctaaaa gcttcccaat gttttgcggc
     2281 ttcataacct ttgcttctag gtcatcttga tttactgtgg gtctgtacgc acggtcaggc
     2341 aatgtaattg ttattcccgc acctttaaaa agttcgacta cattgtcata aattctaccc
     2401 ttaggtagca aaatttttaa ctgttccatc atttacctct tttttggaat actagattat
     2461 cggcaaaaaa aaacgccttc ctcagcagga aggcgcatta ttgcacatca aaggactaaa
     2521 agtcaccagg ctcccgcttt tcgcatttga gaaaaatggc tgtggtgatg ttttctttta
     2581 atcatacttc tattttaaac catttagcgt gcttttgtca agcttcgata cagagctatg
     2641 atttatttac ttcttgtttt aattgcaatt attattatac tcaaggtatg aaacaaaaga
     2701 aaagcttagc agtctgtggt tacaaggctg taaaaacact cgcaaataca aaccctgaaa
     2761 aaattcttcg gttttattat agcaaagaaa gaagtagcga atttggaatg ctctgtaaaa
     2821 aactcgcaga aaataaaatc ccctacaatc aagtagaaaa caacgaactt gaaaaacttt
     2881 caaaaagcct tcatcaccaa ggacttgttg cctttattgc agaaccagaa atagagcagc
     2941 ttagcaagga aaaagtttta gagtttatgc gaaataaaga gaagctcgtc tttttggatc
     3001 ttgttggcaa ctcaaataac cttggggcaa ttattcgtag cgctgctttt tttggcatta
     3061 gacatattat tattcccgaa aatgaaaaac aagcatctat tgcaacaagc acctatcgcg
     3121 ttgcacaggg aggcatggaa tttgtctcac tttactttgt aaaatcccct gagtttttct
     3181 tgcaggatat tgccggaaaa ataattcgca ttgggacaga ccttgaagca aagaaaaaca
     3241 tttctgatat ttcacatatc tatacaccgc aaggagaaaa aaaagcctcc ctcatcattc
     3301 ttggaaatga agaagaaggt atttctccac agataaaaaa tctttgcgac gaactcgtaa
     3361 aaatccctgg gggaaaaaac atgcaaagcc taaatgttgc acaagcagcc tcgattattt
     3421 tttatgagct gacaagaaga taaaaaaacc gaagggccaa aaacctcatc gattcactat
     3481 tgcagatgcg gtaaatagat tattttcgca ggaccaaaaa aatgttccgc caaaaatctc
     3541 acaagtcatt tttacgcttt tgagacctag cccatgtgca tattcttttt tgctcgttat
     3601 cggcaaagcc ttgctgtctt tcgctctttc agcgacacaa gtatttatta ccttaatgag
     3661 tattttatgt atatcgctgt caatttgtat agaaatagaa cgatcttttt cttctacctt
     3721 tatactttct tcgatggcat tttcaaataa attaccaatt atgatacaaa ggtcatagtt
     3781 agttaggtct tttttatttt cagcaagatg ttccgattct aattgtggta gcattgcatc
     3841 tatttctacc ggtatgcaat gcttattgca aataaaaaca taatgcacaa gcaatgcatt
     3901 tacaactacg tttttacaat agagttttgc gttcccctcg tcaattgcac tcggtactga
     3961 gaggattttt tttaatgtat ctatttcgcc cttccctaga agctgcttaa taataccgag
     4021 ttcatgttta taatcatgtc gtataatccg cacatgatca agcatttcag atacacgttg
     4081 ataatacgct tcactcgccg ataaggctat tttcccttcc aaaagcgcaa gctcaaccgc
     4141 tttctttttt tgcacaagta caatgcaaag aatagccatg caatttcctg caagaagaga
     4201 aagtgccaag gaaagagatg tgttcggagt gtacagtgct ttttctgctt caaaaccaag
     4261 caagcgaatc gcataatacg aagcgacagg atacaccagt aaaaaactcc atgccggtct
     4321 atcgagaaaa tctcttaccg cagatataat ttcttttcca aaagatagca ttaacaccaa
     4381 gtaggcgatc attaaaaatt ctattgctaa aaactctgcc attttcccat acacaaggca
     4441 ttcttttcct ataataaaaa tacaatttgc aataagattt tgcacataat gaaaaaatat
     4501 ttgcccgtaa atacttccac ccgaaataag aacgataaca atacaaaaca agaaagtatc
     4561 aagttgcaag gcaagcaaaa aagaattccc tcgtttttct aaaaaataat ccaaaataat
     4621 attgcaaaat aaacaagcta taagagaaag gaaggcgtat ctttttttta tacgtaactt
     4681 aaaagagaaa aggacaacaa taatttgtat acaaactgat gttatgaggc gcgctatatc
     4741 taaataggca tcgatattag acatataata aaacctttta cagatagcgc cgtgcttttt
     4801 taagcacata gtcaacgtag agagacttaa actcactatg gcgtctactt atgggcagtt
     4861 tttcacctgt atgtattatc gcttcattat ttgccacggt ttgaacataa tccatattca
     4921 caataaaaga actatgacag ctcgcaaatc gttcatcttg caacaaaacc tctcccgtcg
     4981 ctgaaagaga accacccgtt ttgagcacct tttgaccttc ttcatttttt atatgaaaat
     5041 acagatagtg cgattttacc tctacccaca taagatgaga aaagagaacc ttctccttcc
     5101 acgacttgct tgataaaaga atgcttgcct catctatttt tctttccttt ttttgctgca
     5161 agtgatcctc gatcgatgaa agcacagaaa aaagtttctt tttattaatt ggctttaata
     5221 agtagctaaa agcatgcacc tcataggatt cacaagcaaa ttcatttgaa cttgtcaaaa
     5281 acacaattgg tccagtgaag tttttctttc tgagctctat tgcaagctct gtaccctttg
     5341 tttctggcat aacaatatca agaatagcag cattcggata aacctcattt ttcaaggttt
     5401 tgagcacagt aagcgtttct tttgcacttg aacaaataca aagccgtaaa ggattctcgc
     5461 ttttggctgt atcagttctt tgcgtattcc attcttggat aaaagaggcc aggttttcta
     5521 attctaaaga attatcatcc accaaaagga ctgtcatata aacagttata atctcttttt
     5581 gccttttttg caataaagcc acttaggaaa gtttaacaac cagttaggaa aaaaaatgct
     5641 ttctcatata aaaatgccat cttttttcta tataatttcc ttagaataag ggcgggttac
     5701 ccgcaaaagg agcattttta tgaaacaaag caaga
//
LOCUS       DAOSSW010000095         5651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_13037_length_5651_cov_5.793781, whole genome shotgun sequence.
ACCESSION   DAOSSW010000095 DAOSSW010000000
VERSION     DAOSSW010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13037_length_5651_cov_5.793781"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..2876)
                     /gene="leuS"
                     /locus_tag="PKW26_07380"
     CDS             complement(237..2876)
                     /gene="leuS"
                     /locus_tag="PKW26_07380"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOQ93424.1"
                     /translation="MAQYPFQEIEKKWQAFWEKNKTFKVKEDASFPKEKRRYVLDMFP
                     YPSAQGLHVGHPEGYTATDIYCRYLRMNGYNVLHPMGYDAFGLPAENYAIKTGTHPSL
                     TTNENIEHFTKQIKSFGFSYDWDRCVSTCTPDYYRWTQWIFLQLYKRGLAYEAETPIN
                     WCTSCLTGLANEEVKDGRCDRCGNTVTRKTIRQWILKITAYAERLLEDLDGLDWSESI
                     KLMQKNWIGKSTGAEVSFMIADADGKATENSLKVYTTRADTLFGATYMVVAPEHPLLE
                     KLTSTEQKKAVENYIEKAAMKSDLERTDLAKDKTGVFTGSYAINPVNNALIPIWVSDY
                     ILISYGTGAIMAVPAHDERDGAFAEKFGLPIIKVVAKPEEVEAIGGEKVIREALEKGS
                     LEELVQNNPKLFAEIQDCFVDEGYSINSGEFSGLSTKEATEAIIEKLSKEGIGEKATN
                     YKLRDWVFSRQRYWGEPIPLVHCESCGIVPIPEDELPLELPNVKSYEPTGTGESPLAG
                     ITDWVNTSCPKCGGKAKRETNTMPQWAGSCWYYLRYLDPQNEKAFASQESIQYWMPVD
                     LYVGGAEHAVLHLLYARFWHKVLYDLGLVNTIEPFQRLVNQGMITSYAFQRKNKMLVP
                     TDMVEENTDGTFTDKETGESLERVIAKMSKSLKNVINPDDVIRDFGADSVRMYEMFMG
                     PLEVSKPWSTQGLVGVHRFLEKVWALSEKPLVDIDLNTIEDTKLIEIRKLFHKTIKKV
                     SEDTENLCFNTAISQMMIFVNEVSKLTELPKALFSDFVKMLNVYAPHLGEELWEKLGN
                     KASVSQENWPQYNEAFCKDDSVTIIVQVNGKIRDKFETAMNTEKEALEKQALSCTAVQ
                     KYLDGNTVKKIIAVPNKIVNIVV"
     gene            complement(2989..3396)
                     /locus_tag="PKW26_07385"
     CDS             complement(2989..3396)
                     /locus_tag="PKW26_07385"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOQ93425.1"
                     /translation="MTLFEWTPEIAVGIQSIDDQHKKLISLINDVSDAMKARKAAQVI
                     GGILQELSDYTVHHFGNEERAFDKYDYPKKEEHKAQHKIYVDKIGELIERNNKGELGI
                     SVSVLDFLMDWITNHIMKIDMQYVPFFSDKEIG"
     gene            3544..4188
                     /locus_tag="PKW26_07390"
     CDS             3544..4188
                     /locus_tag="PKW26_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOQ93426.1"
                     /translation="MQLSKSQSNKNSPFIHYNFSGYLKELLKMFFVFFKIGFVTFGGG
                     MAMLPILERELAQKRGWTTFEDLMDYYAIAQITPGIIAVNVATFLGYKRLGVFGGIIT
                     TLGVVFPSLIVISIVAAFLSSIEHIPEVQHALIGMNIAVAAMLTKILWTFIKTTIKTW
                     KNIFGIVLALLAFVAVAIFKIDSSILILIGALSGLSLHGIQILSKKRKERGQEK"
     gene            4185..4748
                     /locus_tag="PKW26_07395"
     CDS             4185..4748
                     /locus_tag="PKW26_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOQ93427.1"
                     /translation="MSLWELFWVFFYVGLFTIGGGQVAITLMQQIFFSKNLITPERFF
                     NMVAISESTPGPIGINMATYIGYEFYGVWGGVITTLGTVLPSIIIITIIASFFFKIQN
                     KPLVQAAFNGLRPTVTGLIAVAAWLIFRVAVLDLASYAESKHFASLFDIKTIILYLVF
                     LILFFKTKLHPVLFMVLGAVSGILILH"
     gene            complement(4745..5626)
                     /locus_tag="PKW26_07400"
     CDS             complement(4745..5626)
                     /locus_tag="PKW26_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOQ93428.1"
                     /translation="MYILIALIAFILLLIVKNIRIVPQSQAFVIERLGGYLTTWQVGL
                     RIKMPLIDRVANRVSLKERVLDFEPQPVITKDNVTMMIDTVIYLQITDPKLYTYGVEN
                     PMKAIENLSATTLRNIIGDLELDGTLTSRDVINTKMRAIVDEATDPWGIKVNRVEVKN
                     IIPPAGIQEAMEKQMRAERERRESILIAEGKKQSEILVAEGKKQAAILEAEAKKEAAI
                     REAEGEAEAIISVQKATAEGLQMIKSVGLDEKVIKLKSLESFEKVANGQATKLIIPAD
                     LQNMAGLVSSIAEIAKN"
BASE COUNT         1446 a         1133 c         1131 g         1941 t
ORIGIN      
        1 aagaagttac cggttatcgt tgggaatctt ggcattaccg ctacatcggt cttgatgcct
       61 gcctttttca agaagcctat ttcaataata tccaacaata catgcttgag tttatccatt
      121 tgtggaaaaa tctcgattaa tctcgattga agttatttct tcatagcaaa gtaagctaaa
      181 ataaaaactg agagaataga ccaaaaaaaa gcttccacag gggaagcttt tttgttttat
      241 acgactatgt ttacgatctt gtttggaact gcaattattt ttttcacagt atttccgtca
      301 agatattttt gtacagctgt gcatgaaagt gcttgttttt caagtgcttc tttttcggtg
      361 ttcatagctg tttcaaattt atctcgtatt ttaccattta cttgcacgat aattgtgaca
      421 ctgtcatctt tacaaaaggc ttcattgtat tgcggccagt tttcttggga tacgcttgct
      481 ttgttgccga gtttttccca caattcttct cctagatgcg gtgcatagac attgagcatt
      541 tttacaaagt ctgaaaagag cgcctttgga agttctgtaa gttttgaaac ttcatttaca
      601 aaaatcatca tttggctaat tgctgtgtta aagcaaaggt tttctgtgtc ttcacttact
      661 ttttttattg ttttgtgaaa aagctttctt atttcgatga gttttgtatc ttctattgta
      721 ttaaggtcaa tgtctactaa tggtttttca ctcaaagccc atactttttc tagaaaacga
      781 tgcacgccga ctaagccttg cgtgctccaa ggttttgata cctcaagggg tcccataaac
      841 atttcgtaca tacgaacaga gtctgcaccg aagtctcgta taacatcgtc gggattgatg
      901 acatttttca aggactttga catctttgcg attactcgct ctaagctctc tcctgtttct
      961 ttgtcggtaa aagttccatc ggtattttct tctaccatgt cggttggaac gagcatttta
     1021 tttttacgct gaaaggcata gcttgttatc attccttggt ttacgagtct ttggaagggc
     1081 tcgattgtgt ttaccaagcc aaggtcgtag agtaccttgt gccaaaaacg tgcatagagg
     1141 aggtggagga ccgcgtgttc agcgcctccg acatagaggt caacgggcat ccagtattgt
     1201 attgattctt gacttgcaaa agccttctcg ttttgaggat cgaggtagcg caaataatac
     1261 cagcaagaac ctgcccattg tggcatagtg tttgtttctc gttttgcctt tcctccgcat
     1321 tttgggcaag aagtgtttac ccaatcagtg attccagcaa gggggctttc tccagttccc
     1381 gttggctcat agctttttac attgggaagt tccaagggaa gctcatcttc tggaatgggg
     1441 acgatgccgc agctttcgca gtgtacgagg gggatgggtt ctccccagta gcgctggcga
     1501 ctaaataccc agtcgcgaag tttgtaattc gtcgcttttt cgccaatgcc ttcttttgaa
     1561 agtttttcta taattgcctc tgttgcctct tttgtagaaa gacctgaaaa ttctcctgag
     1621 tttatagaat agccttcgtc gacaaagcag tcttgtatct cggcaaaaag ctttggattg
     1681 ttttgcacaa gctcttcaag actgcctttt tctaaggctt ctcgaataac cttttctccc
     1741 ccaatagctt ctacttcttc aggctttgct actaccttta tgatgggaag tccaaacttc
     1801 tctgcaaagg ctccgtctcg ctcgtcatgg gcaggaaccg ccataatagc acctgttccg
     1861 taagagatga ggatataatc agaaacccaa atgggtatga gtgcattatt gactgggttt
     1921 attgcatagc ttcctgtgaa gacgcctgtt ttatcttttg caaggtcggt tcgctcaagg
     1981 tcgcttttca tagcagcttt ttcgatatag ttttctactg cttttttttg ttccgtgctt
     2041 gtaagttttt ctaaaagcgg atgttcagga gcgacaacca tgtaggtagc gccaaagagg
     2101 gtgtccgccc gagttgtgta tacctttagc gagttttcag ttgccttgcc atctgcatct
     2161 gcgatcataa aagaaacttc agctccagtg ctttttccta tccagttttt ttgcataagc
     2221 ttgatagatt ctgaccaatc taagccgtct aggtcttcga ggaggcgttc tgcataggca
     2281 gtgattttga gaatccactg gcgaattgtt tttcgggtaa cggtattgcc acagcggtca
     2341 cagcgaccat cttttacttc ttcgtttgca agccctgtta agcagctcgt acaccagttg
     2401 atgggagttt ctgcttcgta ggcaagccca cgcttataga gttggagaaa aatccactgt
     2461 gtccatcggt agtagtcggg ggtacaggtt gatacacaac gatcccaatc atagctaaaa
     2521 ccaaaagact taatttgttt tgtaaaatgc tcgatatttt cgtttgtggt aagactcgga
     2581 tgtgttcctg tttttatagc atagttttct gcaggtagtc caaaggcatc gtaacccatg
     2641 ggatggagta cattgtagcc attcatgcgt aaatagcgac agtaaatatc tgtggctgta
     2701 tagccctctg ggtgccccac gtgcaaacct tgggctgaag gatagggaaa catgtctagg
     2761 acgtaacgtc gtttttcttt tggaaaggat gcgtcctctt ttaccttaaa agttttattt
     2821 ttttcccaaa aagcctgcca ttttttttct atctcttgaa aaggatactg agccatatac
     2881 tacctcgata aaatgtttgt aaatatatca atgatttttt tgatacttta taattatatc
     2941 tatagtagca taaaaaaagg ctaaggttca agaagaaaat tgtgagttct agcctatttc
     3001 tttatctgag aaaaaaggaa cgtattgcat atcgattttc ataatatggt ttgttatcca
     3061 atccattaga aagtcgagaa cacttacact gatgccgagt tctcctttgt tgtttcgctc
     3121 gatgagttca ccgattttat caacataaat cttatgctgt gccttatgct cttctttttt
     3181 aggatagtcg tacttgtcaa aggctctttc ttcgtttcca aaatgatgta cggtgtaatc
     3241 ggaaagttct tgtaagatgc ctcctataac ttgagcagct tttcgcgcct tcatcgcatc
     3301 gcttacatca tttataagtg aaataagttt tttatgttgg tcatctatgc tttgtatacc
     3361 aaccgcaatt tctggtgtcc attcaaatag ggtcatgagt gtctcctgtt tttagtttca
     3421 aaagttacta ctagtataac acaaaaaagg cataaaggat aggaaaaaat aaaaagtttt
     3481 gacagatggt tttccccttc ttgataaaaa aaagcgaatt gcatacaatt atttttaggt
     3541 ggtatgcaat tgtcaaaatc tcaatcaaat aaaaactccc cctttatcca ttacaatttt
     3601 tctggatact taaaagagct tttgaagatg tttttcgttt ttttcaaaat aggctttgtt
     3661 acctttggtg gtggcatggc gatgcttccc attcttgaac gcgaacttgc gcaaaaacgg
     3721 gggtggacga cttttgaaga cctcatggat tactacgcga ttgcacaaat taccccggga
     3781 attatagcgg tgaatgtggc aacttttttg ggctataaac ggcttggcgt ttttggaggg
     3841 attattacaa cccttggtgt tgtttttcct tctctcattg ttatttctat tgtagctgct
     3901 tttttgtcta gtattgagca tatcccagaa gtgcagcatg cgctcatcgg gatgaatatc
     3961 gctgttgcag ccatgcttac aaaaatactt tggacattca taaaaacaac gattaaaaca
     4021 tggaaaaata tttttggtat agttcttgcg ctgcttgcct ttgtggctgt ggcaattttc
     4081 aagatagact catctatttt aattctcatc ggagccttga gtggtctcag ccttcatgga
     4141 attcaaatcc tatcaaaaaa acgcaaggaa agaggacaag aaaaatgagt ctttgggaat
     4201 tattttgggt attcttttat gtgggacttt ttaccatcgg cggtgggcag gttgcgatta
     4261 cgctcatgca acaaatcttt ttttcaaaaa accttataac gcctgagcgc tttttcaata
     4321 tggtcgcaat ttctgaaagt actcccggtc ccattgggat aaatatggca acttatatag
     4381 gctatgaatt ttatggcgtt tggggtggtg tcataacaac gctcggaaca gtgcttccgt
     4441 cgataattat aattacgata atcgcttcat ttttcttcaa aattcaaaat aagcccctgg
     4501 tacaagcagc tttcaatgga ctcagaccaa ctgttaccgg tcttatcgct gttgctgctt
     4561 ggcttatctt tcgggttgcg gttctagatc tcgcatctta tgcagaaagc aagcactttg
     4621 cttccttatt tgatataaaa acaattattc tataccttgt gtttttgata ctctttttta
     4681 aaacaaaact gcacccagtc ctttttatgg ttctcggtgc agtttctggt attttgattt
     4741 tacactagtt ttttgcaatc tctgcaatgc ttgaaactaa gcctgccatg ttttgtaaat
     4801 cggcaggtat gatgagtttt gttgcctgtc cgtttgcaac tttctcaaag ctctctaagc
     4861 ttttaagctt tattactttt tcatcgagac cgacagattt tatcatttgt aagccttctg
     4921 ccgttgcctt ttgaacagaa ataattgcct cggcttcacc ttcagcttct cgaatagctg
     4981 cttctttctt tgcttcagcc tctaaaatag cagcttgttt ttttccttcc gcaactaaaa
     5041 tttcggattg ctttttacct tctgctataa gaatactttc cctacgttcg cgttctgccc
     5101 gcatttgttt ttccattgct tcttgaatgc ctgctggtgg aataatattt tttacttcga
     5161 cacggtttac cttgattccc caagggtcag ttgcctcatc gacaattgcc cgcatctttg
     5221 tattgataac atcacgactc gtaagggtgc cgtcaagttc aagatcccca attatatttc
     5281 gaagggttgt tgcagaaaga ttttctatag ccttcatcgg attctcgacg ccataggtgt
     5341 agagctttgg gtcggtgatt tgcaaataga taactgtatc tatcatcata gtaacgttat
     5401 cttttgtaat aaccggctgg ggctcaaagt ctaagacacg ctcttttaga gaaacccgat
     5461 ttgctactcg atcgatgagc ggcattttga tgcgtaaacc gacctgccaa gtggtaagat
     5521 agccaccaag ccgttcaata acaaaagcct gggattgagg cacaatgcga atatttttta
     5581 caatgagtaa aagaataaag gcaattaaag caattaaaat atacatattt cctccttatt
     5641 tatgtaacga t
//
LOCUS       DAOSSW010000096         5579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_13268_length_5579_cov_6.670348, whole genome shotgun sequence.
ACCESSION   DAOSSW010000096 DAOSSW010000000
VERSION     DAOSSW010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13268_length_5579_cov_6.670348"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1676
                     /locus_tag="PKW26_07405"
     CDS             198..1676
                     /locus_tag="PKW26_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOQ93429.1"
                     /translation="MVFTKIIAFSIFQALLSTLVAVILGFASAYYVAKKKSLFADILV
                     SFSAVPLCVPPLLVALGYVLFFGNNGSFNRFLMAVFNLQEAPFSILYSFSGIIIAHGF
                     YNFPIIMKAVADVWQRLPEEEEWAASLLGASRFRLFKTISFAHLRSPLASAASIVFLY
                     CFFSFIIVLLFGPIGLTTLEVEVYRASRLSLDFSKAAFFALIETIIALAIVYFYSSFE
                     KSGLRSQGTSAVLREKTKMGLSSRFFSIVLFGLISIFFLFPLFSIPIKAVFTKAAFKT
                     VFFHKSFLPALGASLQSASISALLATSFAFVYALCIRIKDPLKKNRFFRFFPFIPMAI
                     SSVVLGFIITSLLFALRLQANLFILSLCQAFLFWPFAFRQIQFSLDAIPQAIDEVALM
                     LSPSLLYRVYKIYLPLLKTGIISALSFSFAMSLGDASLPLLLAIPNIQTLSLFTYRLA
                     GSYRFAEACASGSIILVLSVFVFYIGERQKKNLKREKKVRMR"
     gene            1673..2383
                     /locus_tag="PKW26_07410"
     CDS             1673..2383
                     /locus_tag="PKW26_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ93430.1"
                     /translation="MKKKDAYFMVENLKKTWENADSAFTLDIDFSVDKGEMLCIAGQS
                     GSGKSTILRIISGILPFEKGARLVLDGKDISTLSPGKRDIGMVFQNPTLFPHMRVEDN
                     IAFGLISRKVAKKDARFRSAALLERFGLSGFAKRYPDSLSGGEAQRVALARTLIVEPK
                     LVLFDEPFSSLDAPLRKSLALDIRQSQRELGFTGILVTHDIEEAKTMSDRVIVLKKGS
                     LFWQGVAKDFEESHVSSF"
     gene            2453..4675
                     /locus_tag="PKW26_07415"
     CDS             2453..4675
                     /locus_tag="PKW26_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOQ93431.1"
                     /translation="MTKKSITRGIVGILMILVVFVSCKSNKNNTDQNTQAIEKPMYRN
                     GDFEDGSVEPWAVFTQGGSARLSVEKGALCIDIGSVGSVEYAVQLYQDVGKLEYGCEY
                     RMQFDMWSTLPRDVEYRIQMNGGNYQAYSMKHISINEEKQSFIIDFTMDQTDDLLPRL
                     AFNMGYPVNLPKGEQVESHKIYLDNLSLTVTDTSNRSEESLGGNKPNISVNLLGYQKA
                     EKKIAVFRSLRMDGKGLDKSFSVFTEEGKKVFTGKVVNPKQNYNANETNAYGDFSELS
                     QTGTYYIESKLAGRSELFHIGDAVYAEAFDAVLKMLYLQRCGSALDVSLAGDFAHPVC
                     HNTLARIYPTDRYIDVSGGWHDAGDYGRYVVPGAKTVADILLAYMHYPHAFDDNLGIP
                     ESGNGIPDVLDEARYELEWLLKMQDKETGGVYHKVTGASFPGFVMPEDETEELILSPI
                     SLTATGDFAAVLAMAVPIYESLDLDFATKMKEAALFAWDYLAANKSFYGFMNPPSIAT
                     GEYDDRKDSDERYWAAVELYKLTGEKRFHDALLSITENVIPTELGWQTVGDYGSISYL
                     TMDVEKQDPEFVARLKTSMDWNIRSLKTLVENDGYGISMGANYPWGSNMTVANNAMRF
                     LFFDMLNQTDEYREIAKNHLHYFFGRNPLMMSYVTGFGNPSPQYPHHRPSEAKKKAMP
                     GMLVGGPNSSLEDPYAKAVLYAEAPAKTYADHSQSYSTNEICIYWNSPLIYVMAALCK
                     "
     gene            4761..4952
                     /gene="rpmB"
                     /locus_tag="PKW26_07420"
     CDS             4761..4952
                     /gene="rpmB"
                     /locus_tag="PKW26_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002677448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOQ93432.1"
                     /translation="MARRCELCGKGTQSGNKVSKAYNHTRRVWKPNLVKVKTEIAGTT
                     MTLKMCTRCLRSGYVTKKV"
     gene            complement(5056..>5579)
                     /locus_tag="PKW26_07425"
     CDS             complement(5056..>5579)
                     /locus_tag="PKW26_07425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOQ93433.1"
                     /translation="VFQKAEKLAMIDPLTGTFNRRYLDLVLQKEINRSERYGKQFSLA
                     IFDIDDFKKINDRYGHVIGDEVLQSFSAYIKATIREEDILCRFGGEEFLIILPETEIE
                     GAMQLCNRIRISFKEEKLTKNISVCFSAGIASFPECGKDSVSLIRQADKALYEAKYSG
                     KDKCVLADKNYNI"
BASE COUNT         1572 a          998 c         1180 g         1829 t
ORIGIN      
        1 tattcccctt acgcagtgga tgtatccggt aaatgaagag gtggttttgc cggcaagttt
       61 tgctatagcg ccaaaggcag aaaaaaacct tcttgtatcg agtgaagtag cactcgaggc
      121 ttccaaacgc cttatggaaa ttttagcaga ttaaaatgac tagaataaaa tgaataaaaa
      181 cacagagggc tattgagatg gtttttacta aaataatagc atttagtatt tttcaagcat
      241 tgctctcaac tctagtggca gttattctcg gttttgcatc ggcctattat gtagcgaaaa
      301 aaaaatctct ttttgcagac attttagtta gtttttcagc ggttcccctc tgtgtgccgc
      361 ccctcttggt tgccctcggc tatgtgcttt tttttggtaa caatggcagt tttaatcgtt
      421 ttcttatggc agtttttaac ttgcaagagg cacccttttc tattttgtat tcttttagtg
      481 gcataattat tgcacatggt ttttataatt tccctattat aatgaaggca gtcgctgatg
      541 tatggcagcg gctgcctgaa gaagaggagt gggcggcttc tcttttgggc gcttctcgct
      601 ttcgtctttt taaaacgatt agctttgcac atttgcgctc tcctcttgca agcgctgctt
      661 ccattgtctt tttgtattgt ttttttagct ttattattgt tttacttttt ggtcccatcg
      721 ggcttaccac gcttgaagtt gaagtctacc gagcaagccg tctcagcctc gatttttcta
      781 aggcagcttt ttttgcgctt atagaaacga ttatcgcttt ggcgattgtg tatttttata
      841 gtagctttga aaaaagcggc ttgcgaagtc agggaacgag tgccgtactc cgagaaaaaa
      901 caaagatggg gctaagctct cgtttttttt ctattgtcct ctttgggctt atttcaattt
      961 tttttctatt tccgcttttt tctattccga ttaaggctgt ttttacaaag gcagccttta
     1021 agactgtctt ttttcacaaa agttttttgc cagcccttgg agcaagcttg caaagcgcga
     1081 gcattagtgc tcttttggca acaagttttg cgtttgtcta tgccctctgt attcgtataa
     1141 aagacccctt gaaaaaaaat cgtttttttc gctttttccc ctttattccc atggcgattt
     1201 cttctgttgt attgggcttt attataacaa gtcttttatt tgcccttcga ttacaggcaa
     1261 atttgtttat tctcagcctt tgccaagctt ttttgttttg gccctttgcc tttcgacaga
     1321 ttcaattctc tcttgatgct attccgcagg ctattgatga ggttgctctg atgctttctc
     1381 cttctctttt atatcgtgta tacaaaatat accttccttt actaaaaaca ggtattattt
     1441 cagccttgag ttttagcttt gcgatgagtt taggcgatgc gagcctgcca cttttgcttg
     1501 ctattccaaa tattcaaacc ctctcgcttt ttacctatcg attggctggt tcctatcgtt
     1561 ttgcagaagc ctgcgcttca ggaagcatta ttctcgtgct gagtgttttt gtcttttaca
     1621 taggcgagag gcagaaaaaa aatctaaagc gagagaaaaa agtgaggatg agatgaaaaa
     1681 aaaagatgca tattttatgg tagaaaacct aaaaaaaaca tgggaaaatg cagattctgc
     1741 ttttacactc gatatcgatt tttctgttga taagggcgag atgctctgta ttgcggggca
     1801 gtcaggttct ggcaagtcga ctattttgcg tattatttct gggattcttc cttttgaaaa
     1861 aggcgcgcgc cttgttctcg atgggaagga tatttcaact ctctctccag ggaagcgcga
     1921 tattgggatg gtttttcaaa acccaacact ttttcctcat atgcgagtgg aagataatat
     1981 tgcttttggt ttaataagcc gcaaagttgc aaaaaaggat gcacgctttc gctctgcggc
     2041 cttgctcgaa cgctttgggc tttcaggttt tgcaaaacgc tatccagatt cgctttcggg
     2101 aggcgaagcc caaagagtcg ctcttgcacg aacgctcatt gtcgagccaa aactcgtgct
     2161 ttttgacgag cctttttctt ccctcgatgc gcccctgcga aaatctttag ccctcgatat
     2221 tcgccaaagt caaagagagc ttggttttac gggaattttg gtaacgcatg atattgaaga
     2281 ggcaaaaaca atgagcgatc gggttattgt tttaaaaaaa ggctctcttt tttggcaggg
     2341 agtggcaaaa gactttgaag aatcccatgt ctcttctttt tagttgacgt acaagaaact
     2401 aaggtttata ctttagttgg ttgaaaacag aaactaagca aggaggatta ctttgaccaa
     2461 aaaaagtata acacgaggca ttgtcggaat tttgatgata cttgtagtat ttgtgtcatg
     2521 taaatcaaat aaaaataata cggatcagaa tacacaggca atcgaaaagc ccatgtatag
     2581 aaatggtgat tttgaggacg gttcggttga gccctgggct gtttttacac aaggtggatc
     2641 agctcgctta tcagtagaga agggggcact ttgtatagat ataggaagtg ttggttcagt
     2701 tgaatatgct gtgcaattat accaagatgt aggtaagctg gaatatggtt gtgagtatcg
     2761 catgcaattt gatatgtggt cgactttacc gcgtgatgtt gaatatcgta ttcaaatgaa
     2821 cgggggaaat tatcaagcct actctatgaa acatatttct ataaatgaag agaaacaaag
     2881 ctttattatc gatttcacta tggatcaaac agatgattta cttccacgtc tcgcctttaa
     2941 tatgggttat ccagtaaatt taccaaaagg ggaacaggtg gaaagtcata aaatatattt
     3001 ggacaatctt tctttaacag ttacagatac ttcgaatcga agtgaagaga gccttggggg
     3061 aaacaagccg aatatttccg taaacctact tgggtatcaa aaggcagaaa aaaagattgc
     3121 agtttttcgc tctttacgaa tggatggtaa aggtttagat aaatccttta gtgtttttac
     3181 cgaagaagga aaaaaagttt ttactggaaa ggtagtgaat cccaagcaaa attataatgc
     3241 aaatgaaaca aatgcctatg gtgatttttc agagctttct caaactggga cttattatat
     3301 tgaatcaaaa ttggcaggaa ggtctgagct ttttcacata ggagatgccg tatacgcaga
     3361 agcttttgat gctgtattaa aaatgctgta tttacaacgt tgtggttctg cccttgatgt
     3421 atcgcttgct ggtgattttg ctcaccctgt ttgccataat acgcttgcac gtatatatcc
     3481 tacagaccgt tatatcgatg tgagtggcgg ctggcatgat gcaggcgact atggtcggta
     3541 tgttgtaccc ggcgcaaaga ctgttgccga tatattgctt gcgtatatgc attatccaca
     3601 tgcctttgat gataatctag gaattccaga aagtggcaat ggtatccccg atgttttaga
     3661 tgaagcacgc tatgaattgg aatggctttt aaaaatgcaa gataaagaaa caggaggggt
     3721 atatcataaa gtaacaggcg cctctttccc tggctttgtt atgccagaag atgaaactga
     3781 agagttaatt ttaagcccca tatcccttac cgccactggt gattttgccg cagttcttgc
     3841 tatggcagta ccaatctatg aatccttaga tcttgatttt gctacaaaaa tgaaggaagc
     3901 agccttattt gcctgggatt atttggcagc caataagagt ttttatggct ttatgaatcc
     3961 gccatcaatt gctaccggtg agtacgatga tcgaaaagat agcgatgagc gatattgggc
     4021 agctgtcgag ttatataagc ttacgggaga aaaacgtttt cacgatgcgc ttttatctat
     4081 aacagaaaat gtaattccta cggaacttgg ttggcaaaca gttggtgatt atggcagcat
     4141 ttcgtatttg actatggatg tagaaaaaca agatccagaa tttgttgcaa gattaaagac
     4201 aagtatggat tggaatatac ggagtttaaa aacgcttgtt gaaaatgatg gttatggcat
     4261 ttcaatggga gcaaactatc catggggaag caatatgaca gtcgcaaata atgcgatgcg
     4321 ctttttattt ttcgatatgc taaatcaaac agatgaatat cgtgaaatag caaaaaatca
     4381 tcttcattat tttttcggac gaaatcctct tatgatgagt tatgttacag gttttggaaa
     4441 tccgagtccg cagtatccgc atcatcgccc ttcagaagcg aagaaaaaag caatgcccgg
     4501 gatgcttgtt ggtggtccaa atagtagttt agaagacccc tatgcaaaag cagtgctcta
     4561 tgcggaagca ccagcaaaaa cctatgcaga tcatagccaa agttattcga caaatgaaat
     4621 ttgtatttat tggaattcgc cacttatata tgtgatggca gctttatgca aataaaattg
     4681 cttgcttcgt tgacattatt ttagcaattt gatagaatac aggttcatat agaattagtt
     4741 tttttatagg caggttaaat atggcaagaa gatgtgaact ttgcggaaaa ggtactcaaa
     4801 gcggaaataa agtcagtaaa gcatacaatc atacacgtcg tgtgtggaaa ccaaatcttg
     4861 taaaggttaa aacagaaata gctggaacga cgatgacatt gaaaatgtgt actcgttgcc
     4921 ttagaagtgg ttacgttaca aaaaaggtct gatagtttcg agtctcttag cttcgatcac
     4981 agtatcgtgg ctttgtgtaa aggctgttag gaagacggag gtttttctac acagtctttt
     5041 ttttatctaa atcttttata tgttgtagtt tttatccgct aagacgcatt tatcttttcc
     5101 agaatatttt gcctcataca aagccttatc tgcttggcga ataagactta cgctgtcttt
     5161 gccacattca ggaaaggaag caatgcccgc agaaaaacaa acgctgatgt tttttgtgag
     5221 tttttcttct ttaaaactta tccgtattct attacaaagc tgcatagccc cttctatttc
     5281 tgtttctgga agaataataa gaaattcttc accgccaaat ctgcatagaa tgtcttcttc
     5341 tctaatagta gctttaatat aggcagagaa ggattgtaag acttcatcgc cgataacatg
     5401 gccatacctg tcatttattt ttttaaagtc atcgatgtca aaaatagcta atgaaaattg
     5461 ttttccatat cgttcgctac gatttatttc tttttgtaaa acaagatcga gataacgacg
     5521 attgaaagtg ccagttaagg ggtcaatcat tgcaagtttt tctgcttttt gaaatactt
//
LOCUS       DAOSSW010000097         5562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_13331_length_5562_cov_5.629018, whole genome shotgun sequence.
ACCESSION   DAOSSW010000097 DAOSSW010000000
VERSION     DAOSSW010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13331_length_5562_cov_5.629018"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PKW26_07430"
     CDS             complement(<1..1061)
                     /locus_tag="PKW26_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOQ93434.1"
                     /translation="MKSFYKIITLSIFSLVIALGVFTSCNKSSKKQLVKDERQKKILV
                     YAYDSFTSDWGPGPALKEAFEKQSDYELELISVGDAGEVLSKALLEKKSPRADVLIGL
                     DNHNAQEAENHGILAIYEPKGSEGIARAIRFSNFLKSKTEWLITPYDWGYFALIYDTE
                     SHLPAPSSLEDLSQEIYKKKLILMDPRTSTPGLGFVSWTYAVFGAEGYEAFWKALKPS
                     ILTMAPGWDSGYGLFTAGEAPLVISYSTSPAYHLEYEDTSRYQALIFDEGHVMQVEGA
                     ALVSGAPNPDGGRAFLDFLISKEAQEIIPLTQWMYPVNEEVVLPASFAIAPKAEKNLV
                     VASEIALEASKRLMEILAD"
     regulatory      complement(1048..1172)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1566..2402
                     /locus_tag="PKW26_07435"
     CDS             1566..2402
                     /locus_tag="PKW26_07435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOQ93435.1"
                     /translation="MKASAGVEYFTLGSIGLLFLFTYNIQHPKWFYPIFATPGLVLTI
                     LISLYKNPEILPAAQSFYFYHKIYTVFIISFIFFYICFKLEKEISETREKTKSNEKQL
                     IYIANHDPLTGLINRRRIWEHFQIYKDRKDLYNSDYSIAIFDIDDFKKINDQHGHDCG
                     DTVLQIISTLIKTIIQKNIKFARWGGEEFILLFPYANPQVIEILENIRIAIENHVFEY
                     KDTKLSITISFGAANSKKYDSINAIIMEADRFLYKGKKQGKNQVVYAPLCFSSEALLQ
                     IV"
     gene            2529..4190
                     /gene="gpmI"
                     /locus_tag="PKW26_07440"
     CDS             2529..4190
                     /gene="gpmI"
                     /locus_tag="PKW26_07440"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOQ93436.1"
                     /translation="MKALEKNSAWKGRRGPVVLVIMDGVGYGKHEEGDAVLASRMAHL
                     DNFKKIAFSTKLKAHGTAVGLPSDDDMGNSEVGHNAIGCGRVFAQGAKLVSNSLQTGA
                     LFEGETWKKLITQVKEKNSALHFVGLLSDGNVHSHIDHLKAMITQAHAEDIQKIRVHT
                     LIDGRDVGETSALDYILPFEAFLADFNTKGRDYKIASGGGRMYITMDRYNADWSMVDR
                     GWKTHVLGEGRLFASAEEAIETYRKEIPGIIDQDLKEFVIKGEDGKALGTVEDGDGLV
                     YFNFRGDRALELTAAFETPEDADFPYFDRKRVPDVFYAGMMEYDGDLHIPNNYLVTPP
                     AIDRTMGEYLAKTGVRTLAISETQKYGHVTYFFNGNKLGKFDEQLETYVEIKSDVVPF
                     EQRPWMKCAEITDYVIEAIASGKYDHIRLNFPNGDMVGHTGVFNAVVCSMEAMDLQLG
                     RLKKAIDAAGGILILSADHGNSDDMYEHKKDGSVSYKEDGTPKAKTSHSLNPVPGIIY
                     DPEYKGEYKKELREGLGISSLAATIIDLLGYKAPEDYDKSLIEYN"
     gene            4467..>5562
                     /locus_tag="PKW26_07445"
     CDS             4467..>5562
                     /locus_tag="PKW26_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ93437.1"
                     /translation="MKKLLTVLLALSLVVSVFAGGSKEKTASNLIELEMYYYKQENQE
                     GLLALLDAYMAKNPGVKIKTLIIPNDADATMAARAAQGKLADILQMQSYSRVHEYAAK
                     GYLVDLSNEDVLKKVVPGSLPSVTYNNKQYALPMDLQGIGIIYNKDLFAKLNLKAPTT
                     YRELENVCNVLKGNGITPFSGLLAENWSAGHFVTLVHTALLAEKGIDFNTFINNMNAG
                     KGSYGEIDTAKLFSTMDFYAANMASNAGDMAWDEQQNDFVAGKAAMMVQGLFALPGDA
                     PFDMGFVPFPVSNTAANNKFYADVDSCFGVSSQSSPEKRKAAIDFLTWLGTPEAQKLW
                     IEGYKLTVSFTGADVSALPPAFTDLIEGVSA"
BASE COUNT         1807 a         1132 c         1026 g         1597 t
ORIGIN      
        1 taatctgcca aaatttccat aaggcgtttc gaagcttcga gcgctatttc acttgctacg
       61 acaagatttt tttctgcctt tggcgcaata gcaaaactcg ccggcaaaac cacctcttca
      121 tttaccggat acatccactg cgtaagggga ataatttctt gcgcttcttt tgaaattaaa
      181 aaatctaaaa aagctcttcc cccatcagga tttggggcgc cagaaacaag ggcagctcct
      241 tctacttgca tcacatgtcc ctcatcaaag ataagtgctt gatatcgact tgtatcttcg
      301 tactccaagt gataggcagg acttgtgcta taacttataa caagcggcgc ttcccccgcg
      361 gtaaataaac catagccaga atcccagccg ggagccatgg taagaattga aggtttcaaa
      421 gccttccaaa aagcttcata tccctcagca ccaaagacag cataggtcca agaaacaaag
      481 cctaaaccgg gtgtgcttgt ccttggatcc atgaggatga gctttttttt atatatctct
      541 tgactgaggt cttctaagga acttggtgca gggagatgag actctgtatc atagataagg
      601 gcaaaatagc cccaatcata gggagttata agccattctg ttttgctttt caaaaagttt
      661 gaaaaacgaa tcgcccttgc tataccctca cttcccttcg gttcatagat agctagaatc
      721 ccatggtttt ccgcttcttg cgcattgtga ttatctaaac caataagtac atcggcacgg
      781 ggggattttt tttctaaaag cgccttactt aaaacttccc ccgcatctcc cactgaaata
      841 agctcgagtt cataatcgct ctgcttttca aaagcttctt tcaaggcagg accaggtccc
      901 cagtctgaag taaaggaatc ataggcataa acaaggattt ttttttgcct ttcatccttt
      961 acaagctgtt tttttgatga tttattgcaa gaagtaaaga ctccaagtgc aataaccaaa
     1021 gagaaaattg ataaggtaat gattttataa aaagacttca ttactttcct ccgctggtat
     1081 tatccagatc aggtttagag cttcatgcga aactccatac gggtattatc tcagagataa
     1141 aacaaggttt tatcacaccc cggtaataat cacagtctag caaataaaaa acaaagcagc
     1201 aagcctagag ataatagaga aaaaaattta attgacaaag ctttataaga catctatact
     1261 tatatggaaa taattgttgt aaataattat ggaggagtaa tatcattcta acaaaagaca
     1321 taaaggcttt atacataaat ttaaaaaaag caccctttgt tttggttttt ttttgtttta
     1381 tagcgcacct ttttttttta cctatttttt tctatttccg agcttttaca ctcttttata
     1441 caagtattct tagtatactt gcctatctct gtattatgcg tttttttaaa tcaataggtg
     1501 agctgtatac agttattttt acttattttt tagtcctatt ctattgcttt acatttcttt
     1561 ttagtatgaa agcaagtgct ggagtagaat actttaccct tggttctatt ggcctccttt
     1621 ttttatttac atacaatatt caacacccaa aatggtttta tcctattttt gcaacacctg
     1681 gtcttgtgtt aacaatacta atctctttgt acaaaaaccc cgaaattctt cctgcagctc
     1741 aaagctttta tttttatcac aaaatataca ccgtcttcat aatttccttt attttctttt
     1801 atatttgttt taaactcgaa aaggaaattt ctgaaacgcg agaaaagaca aagagcaatg
     1861 aaaaacaact catttacatt gcaaatcacg atccactaac gggactcata aatcggcgcc
     1921 gcatatggga gcattttcaa atctataaag atagaaaaga tttatataat tcagactatt
     1981 ccatcgctat ttttgatata gacgatttta aaaaaattaa tgaccaacac ggacatgact
     2041 gcggagacac agtactacaa attatttcaa cactcataaa aacaattata caaaaaaata
     2101 taaaatttgc ccgatggggc ggggaggaat ttatcctctt atttccatat gcaaatccgc
     2161 aagtaataga aatactcgaa aacattcgaa tcgcaatcga aaaccatgta tttgaataca
     2221 aagatacaaa gttaagcata acaataagct tcggtgctgc aaattctaaa aaatatgact
     2281 ctataaatgc aattatcatg gaggccgata gattccttta taaaggcaaa aaacaaggaa
     2341 aaaatcaagt tgtctatgcc ccgctctgtt ttagcagtga agccctattg caaatcgtct
     2401 aaatctgttc agccgctctg ttgaaactcc ttcgtctttt tattacaatt gaacagagag
     2461 taaataaaaa acagctaaac aagccatatt ttattcattt tagattttag aaaaggggtt
     2521 atatttatat gaaagcatta gaaaaaaatt ctgcatggaa gggacgaaga ggccctgttg
     2581 tcctcgtcat tatggacggt gtcggttatg ggaagcacga agaaggcgat gcggttttgg
     2641 catctcgcat ggcacatctc gacaatttta aaaaaattgc ttttagcaca aagcttaaag
     2701 cacacggcac cgctgtcggt cttccatcag atgatgatat gggaaactct gaagttggac
     2761 acaatgccat tggctgtgga cgagttttcg cacaaggcgc caaacttgtt tcaaactctc
     2821 ttcaaacagg tgctctgttt gaaggcgaga catggaaaaa actcatcaca caagtaaaag
     2881 aaaaaaactc tgcacttcac tttgtcggac ttctttccga tggcaatgta cactctcata
     2941 tcgaccacct caaagcaatg attacacaag ctcatgcgga agatattcaa aaaatacgtg
     3001 tacacacact tatcgacgga cgagatgttg gggaaacaag cgcactcgat tatattctcc
     3061 cctttgaagc ttttttagca gacttcaata caaaggggcg tgactacaag attgcttctg
     3121 gcggtggtag aatgtatatc accatggatc gatacaatgc agactggtca atggtagatc
     3181 gtgggtggaa aacacatgtt ttaggagaag gtcgcctctt tgcaagcgcc gaagaagcta
     3241 ttgaaaccta ccgaaaggaa atccctggta ttatcgacca agacttaaaa gaatttgtta
     3301 taaagggaga agacggcaaa gctctcggaa cagtggaaga tggagacggt cttgtctact
     3361 ttaactttag aggggacaga gcacttgaat taaccgccgc ctttgaaacg ccagaagatg
     3421 ctgacttccc atactttgac agaaaacgtg ttcccgatgt cttctatgca gggatgatgg
     3481 aatacgacgg cgatttacac attccaaata actaccttgt aactcccccc gctatcgata
     3541 gaacaatggg agaatatctt gcaaaaacag gagtaagaac acttgccatc agtgaaacac
     3601 aaaaatacgg ccacgtaacc tattttttca atggaaataa actcggcaag tttgatgaac
     3661 agctcgaaac ctatgttgaa attaaaagcg atgttgtacc atttgaacaa cgcccttgga
     3721 tgaaatgtgc agaaatcact gactatgtta tagaagctat tgcaagtgga aaatacgacc
     3781 atattcgatt aaacttccca aatggcgaca tggtaggaca taccggtgtt ttcaatgcag
     3841 ttgtctgctc tatggaagca atggacctgc aactcggtcg cttaaaaaaa gccatcgacg
     3901 ctgcgggtgg catccttatt ttaagcgcag accacggtaa ctctgacgat atgtacgagc
     3961 ataaaaaaga cggctctgtt tcctacaaag aagacgggac acccaaggca aaaacctctc
     4021 actcgctcaa ccctgtcccc ggcatcatct acgatcctga atacaagggc gaatacaaaa
     4081 aagagttgcg tgaaggactc ggcatttcaa gccttgctgc tacgattatc gacttgctcg
     4141 gctataaggc tccagaagac tatgataaaa gcttgataga atataactaa tacaaaaaag
     4201 agaccatcaa attctatgtc gccaaagccc tcccgcaatg cagaataaag cttgtgagag
     4261 gggctttggt ttaaacattc accattttct actaatcttc tatgctaaaa tacaaactta
     4321 cgcaatctac acactttttt caaaaataag gtttttttta attgacaaat cagataaagc
     4381 atgtttaact ataatagctt aaccggttta gtaaaccggt taaaacattg ttaaaactgg
     4441 agatttccag aaactaggag gaaaaaatga aaaaactctt aactgtctta ttggctttga
     4501 gtttagtggt ttccgttttt gctggcggaa gtaaggaaaa aacagcatcg aatcttatcg
     4561 agcttgaaat gtactattac aaacaggaaa accaagaggg tctattagcc ttgcttgatg
     4621 cgtatatggc aaagaatcct ggtgtaaaaa ttaaaacact catcattcca aatgatgctg
     4681 atgcaacaat ggctgcacgc gcagcacaag ggaaacttgc agatattcta caaatgcaat
     4741 catactcacg tgtacatgaa tatgcagcaa aaggttatct tgttgacctt agcaacgaag
     4801 atgttttaaa gaaagttgtt cctggttctc ttccatctgt aacgtataac aacaaacaat
     4861 atgcacttcc tatggactta caaggtattg gtattatcta taacaaagat ttatttgcaa
     4921 aactaaatct taaagcacca acaacctatc gagaacttga aaacgtttgt aatgtgctca
     4981 aaggcaatgg tattacacca ttttctggac ttttagcaga aaactggtct gccggtcact
     5041 ttgtaaccct cgtacataca gctcttcttg ctgaaaaagg aattgatttc aataccttta
     5101 ttaacaacat gaatgctgga aaaggttcct atggagagat cgacactgca aaattgttct
     5161 caacaatgga tttctacgca gcaaacatgg catcaaatgc cggtgatatg gcctgggacg
     5221 agcaacaaaa tgattttgtt gcaggaaaag cagctatgat ggtacaaggt ctatttgcct
     5281 tacctggtga cgctcctttt gacatgggtt ttgttccttt ccctgtatct aatacagctg
     5341 caaataacaa attctatgca gacgttgact catgctttgg tgtatcatca caatcaagcc
     5401 cagaaaaacg aaaagcagca attgacttcc ttacatggct tggaactcca gaagcacaaa
     5461 agctttggat tgaaggctat aaattaaccg tatcattcac cggtgctgac gtttccgccc
     5521 ttccccctgc ctttacagat cttatagaag gagtatctgc ac
//
LOCUS       DAOSSW010000098         5399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_13835_length_5399_cov_4.063249, whole genome shotgun sequence.
ACCESSION   DAOSSW010000098 DAOSSW010000000
VERSION     DAOSSW010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13835_length_5399_cov_4.063249"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2631)
                     /locus_tag="PKW26_07450"
     CDS             complement(<1..2631)
                     /locus_tag="PKW26_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOQ93438.1"
                     /translation="MVLSNFSVKHPAIIMILLVSLVVFALIAFNTLNSEMIPPVGLPQ
                     ANIVTIYPGASAETVEKEVSRIIENEMSTLSGVSSLSSTSSDSFSIVTLEFQDKVKAS
                     EKLPQIRELLYNIEDDFPEGISGTPRIHIVEASAFLPILSLRIESAIEAEELTSYIED
                     FISPSIARIPGVSRISLVGSSKKEAHIQLDIAELEARGISALNVYEVLQYNNLTIPAG
                     NAEYRKKNLSFTSSGSFASLDELKNLTVGAGDGSYIYLKDIATISLKTAPKKIQVRSN
                     QKEYLMLDVLKRDEGDTIKIASATIDIFEKVKKETNGLIDYAIISNQSETTERSLQTV
                     MQSALTGFILTILVILFLLHDVRATIIISLSIPLSILFAILGLWITGRSLNLLSLSGM
                     TVAIGMIVDNSIVVLENTYKHFSKTGDKKKAALSGAGELGGAVLASTTTSVSVFAPLL
                     FLTGIIGVIMNDLSLAIVFALGSSALVAVVIVPWLSSLLLKQKDESEKKKIFLKIENS
                     IDAFMEKIEASYIKSLGWVLQNKKFALVTAIALFIASIILVSILRMNFLPPTDTGEFE
                     IHIETPPGYTLEETQKKVDAIDALVASLVPEIDASVYYVGTDSALSLTDSPNKAFGRI
                     RLVPNEKRKRFIQEIIPLVQDRLSETIPDCNITVLNGGFDSLLALGTGGQGYQLEIYG
                     SDLEKVNSLAITARNFLNQDPDVFKTEISLRSDVEQLIAKLSQSYMGALGVTPYEAGI
                     TSRILFGGMDVGTLHIDNKDYPIRIDSNISDASLNEDTLNKMAILTQDGRYVNFAAFS
                     KLESKPTLTRIDRKDRNYSVEITGFLRVDDQSGVVKRMNEAMSALNLPSEVHYSVSGV
                     SALMQDSISSLVLMFAISIFL"
     gene            complement(2633..3745)
                     /locus_tag="PKW26_07455"
     CDS             complement(2633..3745)
                     /locus_tag="PKW26_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOQ93439.1"
                     /translation="MRKKLRWIIPVIIVILAIGFKIFVSKDTSSVADNAPIPVRVIKA
                     EKGDVSKTLSLNAYVESETMVTILPLVSGIMQELYVDVGDHVTKDAIIARIDAESFSL
                     QLKQAEAAYLSAKSSYERLAQLYKTGATTQQNYEAAKGQYEAYASQWELARLQLNYAN
                     VKSPIDGVVLIKHLSPGSIAAPERPLITVGDISELILRARVPESYYNTIADAKNIPIT
                     IRRMGKEYEGRIKNISPFVSAETKNFEVIISIADPREELRPGMFVEVIFELERWQNVW
                     SLPFTALSSSSKLWWVMDDKAQSLEFYPEKMTENVFLIKPEWADKDFIIEGNWFIYED
                     SPVKIIAPASASEELSLNKKEEKDILSQNHSQTKGG"
     gene            complement(3780..4385)
                     /locus_tag="PKW26_07460"
     CDS             complement(3780..4385)
                     /locus_tag="PKW26_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ93440.1"
                     /translation="MDPTKENILQSALNLMKATGKEGLTLEAVADLAGITRKTLYNHF
                     SGREEIIEEASMLWIESTLNSLKEISEDASLSMAEKINLVMEQGLNEMRSGSRLTRSP
                     RLEAALLRPAKTKIALEKRLHQFVSEIVCLAQEASYIKKEFETESLTLVILNVVEGLC
                     LTEPMEDSPYSATDILRVSLRAILGGVLTEKGLEDLKTLFA"
     gene            complement(4543..5250)
                     /locus_tag="PKW26_07465"
     CDS             complement(4543..5250)
                     /locus_tag="PKW26_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93441.1"
                     /translation="MKKITKGLIILCFLVFASQGIFAQNSDFAIMGKAWGGLNTWPLG
                     TLLTDALKGDLDSNEDLKAYGFSFGGELLFLDPEGFQVGIGLAYLPIISLAIKESGSG
                     YTAQFNGTTSLIPLTADVVFNAGNFYTNLGLGIGWVKAKLKVSTSGSVPLEVQQDIDD
                     TNREIKNFDIGTVFLVKVGTGFNIPVWQNLAIDFGAALYIPFSSQFNEIDEEMSPFLI
                     SLLFSQINLRLGVSYYF"
BASE COUNT         1593 a         1065 c         1057 g         1684 t
ORIGIN      
        1 gaggaaaatc gaaatagcaa acatcagtac aaggcttgaa atagaatctt gcatgagggc
       61 actaacaccg cttaccgaat aatgcacctc acttggaaga ttgagcgcgc tcatagcctc
      121 attcattctc ttaaccacgc cagattggtc atctacgcgt aaaaatccgg taatttcaac
      181 cgagtagttt ctatcctttc ggtcaattcg agtaagtgtg ggttttgact ccagtttaga
      241 aaaggctgca aagtttacat aacgaccatc ctgtgttagg attgccattt tattgagtgt
      301 atcctcgttt aagcttgcat ctgaaatatt tgaatcgata cgaataggat agtccttgtt
      361 atcgatatgc agtgtaccga catccatgcc gccaaataaa atacggcttg tgattccggc
      421 ttcgtagggg gtaacgccga gggcacccat gtaggattgg ctgagttttg caatgagttg
      481 ttcgacatcg gatcgaagag aaatctctgt tttaaagacg tcagggtctt ggtttaaaaa
      541 atttcgcgcg gtaatagcga ggctatttac cttttctaaa tcgcttccat aaatttcgag
      601 ttggtagcct tgccctccgg ttccgagcgc aagtaaagaa tcaaagccgc cattgagcac
      661 ggtgatattg caatcgggaa ttgtttcaga aagtctatct tgcacgaggg gaattatttc
      721 ttgaataaat cgtttgcgct tttcatttgg tacgagacga atgcgcccaa aggctttgtt
      781 cggggagtca gtaagcgaga gtgcggaatc ggttccgaca taatacactg acgcatctat
      841 ttctggaacg agggaagcta caagggcatc gatggcatct actttttttt gagtttcctc
      901 gagtgtgtat ccagggggag tttctatgtg aatttcaaac tcaccggtat ctgttggtgg
      961 caaaaaattc attcgtaaaa tagataccaa aataatactt gcaataaaga gagctattgc
     1021 tgttacaaga gcaaatttct tgttttgtaa aacccagcca agtgatttaa tataagacgc
     1081 ctctattttt tccataaagg catcgatact gttttcaatt ttcaaaaaga ttttcttttt
     1141 ttcactttca tctttttgtt tcaatagtaa gcttgaaagc catggaacaa tgaccaccgc
     1201 gacaagggct gaggagccaa gtgcaaaaac aattgcaagg gaaaggtcgt tcataatgac
     1261 gccgattatg cccgttaaaa aaagcaaggg agcaaaaacc gaaacgctcg tagtcgtgct
     1321 tgcaagaaca gctccgccaa gttctcctgc cccagagagc gccgcctttt ttttatctcc
     1381 tgtttttgaa aaatgcttgt aggtgttttc caaaacaaca atggagttgt caactatcat
     1441 accgatagcg acagtcatgc cagaaaggga taaaaggttt agcgaccgtc ctgttatcca
     1501 taagcccaag atagcaaaaa gaattgagag aggaatagag agggatataa taatagttgc
     1561 ccgaacatcg tgaagtaaaa aaaggataac taaaatagtg aggataaaac cagtgagcgc
     1621 agactgcata actgtttgca aggagcgctc tgttgtttca ctttgattgg aaataatcgc
     1681 ataatcaatg agaccatttg tttctttttt taccttttca aatatatcga ttgttgccga
     1741 agcgattttt atcgtatctc cttcatcacg ttttaaaacg tcgagcatga gatattcttt
     1801 ttgattagac cgtacctgta ttttttttgg tgcggtttta agcgatatag tcgcaatatc
     1861 ttttagatag atatacgagc catctcctgc gccgactgtg agatttttta attcatctaa
     1921 acttgcaaaa gaacctgagc ttgtaaaaga aagatttttc tttcgatact ctgcattccc
     1981 tgcgggaata gtgagattgt tgtattgtaa aacttcgtag acattgaggg cagaaatacc
     2041 acgagcttca agctctgcta tatcaagctg gatatgagcc tcttttttgc tacttcctac
     2101 caagctaatg cgtgaaacac cgggaatacg tgcgatggag ggcgagataa aatcttctat
     2161 gtagctagtt aattcttcag cctcgatcgc gctttctatt cgaagagata atatgggcaa
     2221 aaaagccgaa gcttcaacga tgtgaattcg tggcgttccc gatatgcctt ctggaaaatc
     2281 atcttcgata ttgtagagaa gttcacgaat ctgtggcagt ttttcgcttg ccttaacctt
     2341 atcttgaaat tcaagcgtta cgatagaaaa tgaatcagag ctggtagagc ttaaactact
     2401 tacccctgag agagtggaca tttcattttc tataatccgc gagacttcct tttcaacagt
     2461 ttctgcactt gctccaggat atatcgttac gatatttgcc tggggtagac caacgggagg
     2521 tatcatctca gagttcagcg tgttaaaggc gatgagtgca aaaacaacga ggctgacaag
     2581 gagtatcata ataattgcag gatgctttac agaaaaattt gataaaacca ttttagcctc
     2641 ccttggtttg ggagtgattt tgacttagaa tgtctttttc ttctttttta ttcagggaga
     2701 gttcttctga tgctgaggca ggagcaatga tttttacagg agaatcttca tatataaacc
     2761 aattcccctc gatgataaaa tctttatctg cccactcagg tttaattaaa aaaacatttt
     2821 cggtcatttt ttcaggataa aactctaggc tctgtgcctt atcgtccata acccaccaaa
     2881 gctttgaact agaactaagt gcggtgaagg gaagagacca tacgttttgc catcgctcaa
     2941 gttcaaaaat aacttctaca aacatgccag ggcgaagctc ttctctcggg tctgcaatac
     3001 taataattac ttcaaagttt tttgtttctg ccgaaacaaa gggcgagata tttttaatcc
     3061 gtccctcata ttcttttccc atgcggcgta tagtaatggg aatgtttttt gcatcagcga
     3121 tagtgttata gtaagattct ggaactcgag cgcgaagaat gagctcggaa atatctccaa
     3181 cagttatgag aggacgctca ggggcagcaa tgcttcccgg actaaggtgt tttatcaaaa
     3241 caactccgtc tatcgggctt tttacatttg cataattgag ttgcaaacgt gcaagctccc
     3301 attgggaagc gtaagcttca tactgcccct tcgctgcctc gtagttttgt tgtgttgttg
     3361 cccctgtttt atagagctgg gcaaggcgct cataacttga ctttgccgaa agatacgcag
     3421 cctctgcttg cttcaattgc agagaaaaac tttctgcatc gatacgtgca atgattgcat
     3481 ctttggtaac atggtctccc acatctacat aaagctcttg cataatgccc gaaacaagag
     3541 gcaaaatagt taccatagtt tccgattcta cataggcatt taaggaaagc gttttactta
     3601 catcgccttt ttctgccttt ataacacgta caggaatagg tgcattatct gcaacagagc
     3661 ttgtatcttt tgatacaaag attttaaaac caattgcgag aattacaata ataacgggaa
     3721 taatccaacg aagttttttt cgcatattta cctcgataaa aaacaaggtt tagatttttt
     3781 tacgcaaaga gcgttttgag atcttctaaa cctttttctg ttaacactcc acctaaaata
     3841 gcccgtaagg atacgcggag aatatctgtt gcgctatagg gtgaatcttc cattggttca
     3901 gttaaacaaa gtccctcaac cacattgaga ataaccagtg tgaggctttc tgtttcaaat
     3961 tcttttttta tataactggc ttcttgagct aagcagacaa tttcacttac aaattgatgt
     4021 aagcgttttt ccaaagctat ttttgtcttt gctggacgta aaagcgctgc ttctaagcgc
     4081 ggagagcggg ttaatctact tcccgagcgc atttcattta ggccctgttc cattacaagg
     4141 ttgatttttt cagccatgga aagagatgcg tcttcgctaa tctcttttag agagtttaga
     4201 gtcgactcaa tccaaagcat tgatgcctct tctataatct cctcgcgacc tgaaaaatgg
     4261 ttataaaggg tttttcttgt aattccggca agatcagcta cagcctcaag ggtaagcccc
     4321 tctttaccag tcgctttcat aagatttaga gcagattgta aaatattttc ttttgttgga
     4381 tccatctgaa cctcctggat tacacaaaag ttacttcttg tgtgtagtat gtatcagttt
     4441 tctttctttg tcaagcgctt atttttaaaa aaaaagccac ttgcaattta aattaaaaac
     4501 tgcaaatagc tttttataag aaaattactg tatgttacgt aattagaagt agtaacttac
     4561 tccaagtcgt aggtttattt gtgaaaataa taggcttatt aaaaagggac tcatctcttc
     4621 atctatctca ttaaactgcg aactaaatgg tatatagaga gctgcaccaa aatcaatggc
     4681 tagattttgc caaacaggaa tattgaaacc ggtccctact tttacaagaa aaactgtgcc
     4741 aatgtcaaaa ttcttaatct ccctattcgt atcatctatg tcttgttgca cttccagtgg
     4801 tactgagcca ctagtcgaaa cttttaattt tgctttaacc cagccaattc caagtcctaa
     4861 gttggtataa aaattacccg cattaaaaac cacatctgct gtaagaggaa ttaagctcgt
     4921 tgttccattg aattgtgcag tatatccaga accgctttcc tttatagcta atgatattat
     4981 tggaagatat gcaagtccta ttccaacttg aaaaccctct ggatctaaaa ataaaagctc
     5041 tccaccaaaa gaaaatccat aggcttttaa gtcttcatta ctatcaagat cgccttttaa
     5101 tgcgtctgtt aatagtgtac ctaagggcca ggtatttaag ccaccccaag ccttccccat
     5161 aatagcaaaa tcagagtttt gcgcaaaaat cccttggctt gcaaagacta gaaagcataa
     5221 gataatgagt ccttttgtaa tttttttcat atttttctcc tagaattatg tattctcctg
     5281 aatactatta tagcaggcta tataaaaaaa acaaggatat ttacaaaata attgattttt
     5341 atatcagttt tcttgacaat ttacctttct cgtattatag tataattaca taggaggtt
//
LOCUS       DAOSSW010000099         5266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_14319_length_5266_cov_5.132796, whole genome shotgun sequence.
ACCESSION   DAOSSW010000099 DAOSSW010000000
VERSION     DAOSSW010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14319_length_5266_cov_5.132796"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PKW26_07470"
     CDS             <1..378
                     /locus_tag="PKW26_07470"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757994.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine hydroxymethyltransferase"
                     /protein_id="HOQ93442.1"
                     /translation="DVTTFGLTGRQAESALVECGLTLNRNSLPFDPNGAWWTSGIRIG
                     TPATTSLGMGKTEMAEIASIIHLVLSNTKPAMTEKAVPAKGKYILDEATKTEAKKRVN
                     TLLDAFVLYPELDLDFLKKSFVK"
     gene            complement(459..842)
                     /locus_tag="PKW26_07475"
     CDS             complement(459..842)
                     /locus_tag="PKW26_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOQ93443.1"
                     /translation="MCEKKFYICKHCGNIVGMIHASGVPIVCCGENMTELVANTTEAS
                     TEKHLPEVKIEGSKVTAVVGSVEHPMEEKHWIQWIYLATNKAGHRYCLNPGDKPEAVF
                     HLAEGETPLAVYEYCNLHSLWLTKL"
     gene            1025..1279
                     /locus_tag="PKW26_07480"
     CDS             1025..1279
                     /locus_tag="PKW26_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOQ93444.1"
                     /translation="MLVSLIAIGNSKGIRIPKTVIDALEIEDSLELRIKDKSIILEPI
                     KSKPRQGWEEAFAREYDDDDVLEDVLFVADDNSEDFEWEW"
     gene            1267..1593
                     /locus_tag="PKW26_07485"
     CDS             1267..1593
                     /locus_tag="PKW26_07485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOQ93445.1"
                     /translation="MGMVNQYEVHLINLDPTIGHEIKKTRPCLVISPNEMNSSVRTFI
                     IAPMTTKAHDYPTRLGVFFKGKNGWIVLDQIRAIDSHRLVKKLGTIDKETIKKVKMVL
                     KEMLVD"
     gene            complement(1599..2132)
                     /locus_tag="PKW26_07490"
     CDS             complement(1599..2132)
                     /locus_tag="PKW26_07490"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOQ93446.1"
                     /translation="MFNVHELDKAIRRVPNFPKEGILFYDITSILTNPRVFASCIEEM
                     KRIYQDAHIDAIAGIESRGFLFAAPFAHALGVPLILIRKKGKLPGEVYSCKYDLEYGS
                     AEIEAHKADIQKGQRVLVIDDLIATGGTLKAAQKILEAGGSEVVEFFGIVGLPFLNYD
                     KVLTPIKVRTLINYDSE"
     gene            complement(2220..3167)
                     /locus_tag="PKW26_07495"
     CDS             complement(2220..3167)
                     /locus_tag="PKW26_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HOQ93447.1"
                     /translation="MMQKKQVGEKKSDCKNKDKKDSTETVFTIGFVGLGLMGGSFAKA
                     IQKAHPSYRLLAYDKNPDTLEKAKKQGLIAEGFSSQPELMLIHCDLVYINLYPGMVAD
                     FIIEHEKDFKSAAIISDISGVKTKIAEKLSCLSRKDIAIVLGHPMAGSEKEGFDHANE
                     SIFLGRNYIFIEDVAYAEANPELHKKAIEQLKELALAIGFSRTIETSATMHDHKIAFT
                     SQLCHVIASALVQSAEDTEITAFGGGSYEDLTRIAMINAPLWTELFLSNNTELVKHIE
                     IFEKYLDNLKNLISEKNKESLEKNLHEVRKKRIAMNGKK"
     gene            complement(3164..4192)
                     /gene="aroF"
                     /locus_tag="PKW26_07500"
     CDS             complement(3164..4192)
                     /gene="aroF"
                     /locus_tag="PKW26_07500"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOQ93448.1"
                     /translation="MIIALKSGIAKSDVQNFVSTLEGQGFQIHLSEGIDTTILGLVGD
                     TSRLSEESFKAHPLVSKVMRVQEPYKKANRAFHPDNTIISLPSGKTGGMADIGFPNFS
                     VMAGPCSVESEEQIIEVAKSVQRSGARFLRGGAFKPRTSPYAFQGMGVEGIDLLLQAK
                     KETGLPIVTELMAINQLEVFENVDIIQVGARNMQNFNLLKELGHSKKPILLKRGLANT
                     VKELLMSAEYIMAGGNENIILCERGIRSFDNFTRNVLDLAAIPYLKKVSHLPVIVDPS
                     HSTGLAWMVESMSKAALAAGADGIIVEVHNCPECALCDGEQSLNPVEFENLMQKLTKY
                     AAIEGLNL"
     gene            complement(4466..>5266)
                     /locus_tag="PKW26_07505"
     CDS             complement(4466..>5266)
                     /locus_tag="PKW26_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93449.1"
                     /translation="HAVQSPSAYMGYRAFISMGEDNNLEHCLPKEEIQEVGHYIDHEI
                     VSNIEYDCSLRLARLKKGCSRRFLLTMGGAGAQVQKFADIVGASKDAIRNEKATFFIN
                     MGDHKGRWEALEAEFKKAGISYTLHDTWKESRSFIDEHREKPVFGVHVFLHENFYAAV
                     YATNLLMRISDVMITKPSELSFYPVPKLFIQRVGKHEAWGAIRGSEIGDGTIETGSVT
                     SMHRTLKLLIEDTDLIELYCDHIVKNYKQGIYNGAYNTVDLALKLQGR"
BASE COUNT         1479 a         1104 c         1043 g         1640 t
ORIGIN      
        1 gacgttacta cctttggtct aactggtcga caagcagaaa gcgcccttgt agaatgcggt
       61 ctaacactca atcgcaactc tcttcctttt gatccaaacg gcgcctggtg gacaagtggt
      121 attcgcattg gaactccagc aaccacaagc cttggcatgg gcaagacaga aatggcagaa
      181 attgcttcga ttatccatct tgttttatcc aatacaaagc ctgctatgac agaaaaagct
      241 gtacctgcaa agggaaaata catcctcgat gaagcaacca aaactgaggc aaaaaaacga
      301 gtcaatacct tgctcgatgc ctttgtgctc taccctgaat tagacctcga tttcttaaaa
      361 aaatcttttg tgaaataaaa atcgatttga atcacttaag ataaaaaaag gtgtctgcta
      421 ttctacgcag ccaccttttt tttgctatac agctttattt agagctttgt aagccataaa
      481 ctgtgaagat tgcagtattc atagacggca agaggtgttt ccccttctgc gagatgaaaa
      541 acagcttctg gtttatcccc tggattgaga cagtagcgat gccctgcctt attcgttgcc
      601 aaataaatcc attgaatcca atgtttttct tccatagggt gttcaactga gccaacaaca
      661 gcagttactt ttgaaccttc tatttttact tctggaaggt gtttttcggt gcttgcttca
      721 gttgtatttg ccacaagctc agtcatattt tcgccacagc aaacgattgg tacaccagac
      781 gcatgaatca tcccaacgat gtttccacaa tgtttacaaa tgtaaaattt tttttcacac
      841 atagtttttc tctaaaccaa gacccctctt atttgaagag aagttctttg acttagtttc
      901 ctccttataa ttaaaatccc tattagtata ccatggaata agaaaaaaac aaagagaaaa
      961 aaggaagtat ttttattgct ttcttttagt aattacatta taattacatt tgacaggagg
     1021 aagtatgtta gtatcactta ttgccattgg taattcaaag ggaatccgca ttccgaaaac
     1081 agtgatcgat gcactcgaaa tcgaagattc acttgaattg cgaatcaaag acaagtcaat
     1141 tatcctcgaa ccaataaaat ctaaaccacg acagggctgg gaagaagcat ttgctagaga
     1201 gtatgacgac gacgatgtac tcgaagatgt attgtttgta gcagatgata actcggagga
     1261 ctttgaatgg gaatggtaaa tcagtacgag gtgcatctta taaatctaga tccaacaatt
     1321 ggtcatgaaa taaaaaaaac acgtccctgt cttgttattt ctccaaatga aatgaactct
     1381 agtgtgcgta cgtttataat tgctccgatg acaacaaagg cacatgatta tcctactaga
     1441 ctcggcgttt tttttaaagg taaaaatggc tggatagtac tcgatcaaat tcgcgcaata
     1501 gatagtcata gactcgtaaa aaaactgggt acaatcgaca aagaaacaat taaaaaggta
     1561 aaaatggtat taaaagaaat gcttgttgac taagcgcttt attctgaatc gtagttaata
     1621 agtgtgcgta cttttatcgg ggtgaggact ttgtcgtagt tcaaaaaggg aagtcctaca
     1681 atgccaaaaa actcaacaac ttcagatccg cccgcttcaa gtattttttg tgctgctttg
     1741 agcgtgcctc ctgttgcaat gaggtcatca ataactaaaa ctcgttgccc tttttgaatg
     1801 tctgctttat gggcttctat ttctgcactg ccgtattcaa gatcatattt gcacgaatag
     1861 acttctccgg gaagttttcc tttttttcgt atgagaataa gagggacgcc aagagcatgt
     1921 gcaaagggag cggcaaataa aaaaccacga gattcaatgc ctgcaatcgc atctatgtga
     1981 gcatcttgat aaatgcgctt catctcttca atgcaagaag caaagaccct tgggttggta
     2041 agaatacttg ttatgtcata aaaaaggatg ccttcttttg ggaagtttgg cacccgtcga
     2101 attgctttat caagctcgtg aacattgaac atagctttta tcatagcgag cggacgagga
     2161 agagtcaagg gacctcaaag caaagcaagg aaatctgatg cactcaaaat ctttatgtct
     2221 tattttttcc cgttcattgc aattcgtttt tttcgcacct cgtgcaagtt cttttccaaa
     2281 ctttctttat ttttttcgct tatgaggttt ttcaagttgt ctaggtattt ttcaaagatt
     2341 tctatatgtt tgacgagctc ggtattgttt gaaagaaaaa gttctgtcca cagtggtgca
     2401 tttatcatcg caatacgggt aaggtcttcg tagctgcctc ctccaaaggc ggttatttcg
     2461 gtgtcttctg cgctttgcac caaggcagat gcaatgacgt gacagagttg actcgtaaat
     2521 gcaattttat ggtcgtgcat tgtagcgctt gtttctatcg tgcgtgaaaa acctatcgcg
     2581 agagcaagct ctttgagttg ctctattgct tttttatgaa gctcgggatt tgcttctgca
     2641 taagctacat cttcaataaa aatataattt cttccaagaa aaatgctttc atttgcatgg
     2701 tcaaagcctt ctttttcact tcctgccatg ggatgaccga ggactatggc tatatctttt
     2761 cggctgaggc agctcagctt ttcggctatc tttgttttta cgccacttat atcgcttatg
     2821 atggcagcgc ttttaaaatc cttctcgtgt tcaataataa agtctgcaac cattcctgga
     2881 tacagattga tatataccag atcgcagtgg atgagcatga gttcaggctg gctactaaag
     2941 ccctctgcga tgagaccttg tttttttgct ttttcaaggg tgtcaggatt tttgtcataa
     3001 gcaagtaaac ggtacgaggg atgcgctttt tgaattgcct tggcaaagga gccacccatg
     3061 agaccgagcc ccacaaagcc tatggtaaag actgtttccg ttgagtcctt tttatctttg
     3121 tttttgcaat cgcttttttt ttcaccaacc tgtttttttt gcatcataga ttgagtccct
     3181 caattgcggc atacttggtt aatttttgca taaggttttc aaactctact gggtttaagg
     3241 attgctctcc atcacagagg gcacattctg ggcaattatg cacttcgacg attatgccgt
     3301 cggcaccagc tgcaagggct gcctttgaca tagactcaac catccaagca agcccagtcg
     3361 agtggcttgg gtctacgatg actggcagat gactcacttt ttttaggtag ggaatcgcag
     3421 cgaggtcgag tacatttcgg gtaaaattgt caaaacttcg aatgccccgt tcacataaaa
     3481 taatattttc attgccgcct gccattatgt attccgctga cataagcaat tctttaacgg
     3541 tgtttgcaag ccctctttta agtaagatag gcttttttga atgaccgagt tctttgagca
     3601 agttaaagtt ttgcatattg cgagcgccaa cctgtataat atcaacattt tcaaaaactt
     3661 cgagttgatt tattgccata agctcggtta cgatggggag acccgtttct ttttttgctt
     3721 gcaaaagcaa atcgattccc tccacaccca taccttgaaa ggcatagggc gaagtgcgtg
     3781 gcttaaaagc tcctcctcgt aaaaatcgag cgcccgatct ttgtaccgat tttgccactt
     3841 ctatgatttg ctcttcgctt tctacagagc agggaccagc catcacagaa aagtttggaa
     3901 aaccgatgtc agccataccg cctgtttttc cgcttggaag agaaataatc gtattatccg
     3961 gatgaaaggc tctgtttgcc tttttgtatg gctcttgcac acgcataacc tttgacacca
     4021 agggatgcgc cttaaagctt tcttcgctta agcgacttgt atctcctaca agcccaagaa
     4081 ttgttgtatc gattccctca gagaggtgga tttgaaaacc ttgtccctca agcgttgaaa
     4141 caaagttttg cacatcgctt tttgcaatgc cagattttaa tgcaataatc attttactta
     4201 ctccttcagt ttttttcaaa acactgcttg cacaaaaaaa aagaaccccg aattcggagt
     4261 tctgttattt ttgcctttat aatacaggtt actataaacc tattttaaaa gcaggctccg
     4321 aagcataaag agaattccag tagtaataaa agtataccat ttgagaagat ttggattgtt
     4381 ctatttgtgc atgagctgct ttcataatgc taataatagc aaagacgaga gaagcctgtc
     4441 aagagcctgc ttgtatgcgt aaaaattatc tcccttgtag tttcaaggca aggtcgacag
     4501 tattgtatgc cccattgtaa ataccctgtt tataattttt tacgatatgg tcgcagtaga
     4561 gctcaatgag gtctgtatcc tctatgagca gttttaaagt gcggtgcatg cttgttacag
     4621 agcctgtttc aatcgtgccg tctcctattt cgctaccacg aattgcaccc caagcctcat
     4681 gttttcctac cctttgaata aagagctttg gtaccggata aaaagaaagt tcactgggct
     4741 ttgtaatcat tacatcgctt atcctcatga gtaggtttgt cgcatagacg gctgcataaa
     4801 agttttcatg taaaaataca tgcactccaa aaacaggttt ttcacggtgt tcatctataa
     4861 aggagcgact ttccttccat gtatcgtgaa gtgtataaga aatccctgct ttcttaaatt
     4921 cagcttcgag cgcttcccaa cgccccttgt ggtcgcccat attgataaaa aatgtcgctt
     4981 tttcatttct tattgcatcc ttgcttgcgc ccactatgtc ggcaaacttt tgcacctgcg
     5041 ctcccgcccc acccatggtg agcaaaaagc gtctcgaaca gcctttttta agccgagcaa
     5101 gacgtaaaga gcaatcgtat tcaatatttg aaacaatttc atggtctata tagtgcccaa
     5161 cttcttgaat ctcttctttt ggaaggcaat gctcaagatt attatcctct cccatggaga
     5221 taaatgcacg gtagcccatg taggctgagg gcgactgcac ggcatg
//
LOCUS       DAOSSW010000100         5146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_14728_length_5146_cov_7.453742, whole genome shotgun sequence.
ACCESSION   DAOSSW010000100 DAOSSW010000000
VERSION     DAOSSW010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14728_length_5146_cov_7.453742"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PKW26_07510"
     CDS             complement(<1..746)
                     /locus_tag="PKW26_07510"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93450.1"
                     /translation="MRALQKNKVIFILAFFFFTGLHANPFLSTSSPTEETEQTERESL
                     QFENAHQEQVEKEEGKPKTTPQIRLGQTSEKNLEVQRGLRETLGNSFYEWKALQESGN
                     TADQKKAESIFWTILAAAFAFGLFHALGPGHRKTIVFSLYISRKAAWWEPLAVALSLS
                     ALHAGSAIVLILFLNGITGSISARADTISKWMEGLSYIILILSAIALLSHSIVEQISY
                     AKKTKAGTNKSEGRKNISLSAFIFSGIYPCP"
     gene            complement(758..1414)
                     /locus_tag="PKW26_07515"
     CDS             complement(758..1414)
                     /locus_tag="PKW26_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HOQ93451.1"
                     /translation="MQLKLRAYKKIYFSLFLFILLSLTPLFAHPHVAIESKLNFVFED
                     NKLEGVYVEWKFDHFFSADIISWLDVDKNGQFDEKESVEVYNNAFINLRHYYYYTFIR
                     QGSERTNPAKVEQFRARIEKGQMIYRFYIDLSQYPKGELYFANYDYTFFTDVSYPESA
                     GIEFTLKNSSIKPQYSISANKEYPVYYNPLGAIDDTRVYYKWEKGLQTYYPIEVKISY
                     "
     gene            complement(1434..3833)
                     /locus_tag="PKW26_07520"
     CDS             complement(1434..3833)
                     /locus_tag="PKW26_07520"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOQ93452.1"
                     /translation="MKETFTVEGMSCAACSSAVERSVRKLEGIKEANVNLTTKKLSVE
                     FDSGQSISKDEIIKAVTKAGYSASLEAEQKPMEKNSSQVNASLEDASIKVKQLKKELF
                     GAWLFTIVLIYVSMGQMLAKPLPLFSIFSMHALPMNFAVLQLLLTIPVLYFARAYFYR
                     GFKSLYYRAPNMDSLIAIGSLASFVYSLYLTFLISLDASHVHNLYYESAASVLTFIMT
                     GKFLEAKSTDKTTSAISSLIKLSPPKATLVAGYDDPSLMLPPRAVQLEEIVVGNVLLI
                     KPGETIPLDGIVLEGESDVNEAMITGESMPVSKEEGDSLIGGTLNTSGVLFMRVTRTG
                     KDTTLAKIISFIEETQGKKAPIARLADIVSGIFVPVVLAIALVSAIIWILSGAEAHFV
                     IRVVTSVLVIACPCALGLATPTAIMVGTGLAASNGILIRTPEVLELLKKTTAVVLDKT
                     GTITKGKPVVKSISLTKDAQESLRFYQDKINPKQDSIDEEKIILALAYAIEKNSSHPI
                     AHAIVEEAKKRGIDENTLTQMNILLESETNLSGFGLSANLIQENKRLSLLVGNERLMN
                     TEKIAVDALAEEAEKQQDEGASLMYLAIDTKLLALIAIEDEIKEDSIQAIQALKKMGK
                     KIILLSGDNKKSAAYVASLVGADQVIAEVLPEDKAEVIKDLQEKGEIVLMAGDGINDS
                     PALVQADIGAAMGKGSDTAIEAGDIILMNSNISSIKDALVLSRLTLRVIKQNLFWAFL
                     YNTIGLPLAAGLLYPRFGLLLSPMFAGFAMAMSSISVVSNALRLRLIYGRKKHALDKK
                     K"
     gene            complement(3933..4220)
                     /locus_tag="PKW26_07525"
     CDS             complement(3933..4220)
                     /locus_tag="PKW26_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93453.1"
                     /translation="MEYVYIKDSEGYVQKKLKTELQQNDTIISETEYKKKSGLSFYEK
                     TFTHGGVRKNAGRKQKYKQALKYQMRVTEEEKEFISYVREHHIDYKTLLKK"
     gene            complement(4223..4519)
                     /locus_tag="PKW26_07530"
     CDS             complement(4223..4519)
                     /locus_tag="PKW26_07530"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOQ93454.1"
                     /translation="MIFKIKIQNFAQFEISKLEEKQQDVLSKDYTLIETQGIEYVRIK
                     HLQGNIFEIKSKEIRSLFKYCSGQIIIIGVVFVKKTKKTPKEIIKLAKKRLKEV"
     gene            complement(4597..4992)
                     /locus_tag="PKW26_07535"
     CDS             complement(4597..4992)
                     /locus_tag="PKW26_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93455.1"
                     /translation="MYPEIKNHPLFSLEHILTTGLLPPFFLTSEASTVEEDLSDYVNT
                     YLKEEIAAEGLSRSLSGFARFLEVAALSNTGIINYTNIANDAQIPRQTVRLWFEILYD
                     TLLAFELPVFRKTIKRKWYRTHALASFFR"
BASE COUNT         1373 a          979 c         1058 g         1736 t
ORIGIN      
        1 gggcaggggt agatgccaga aaaaataaat gcgctaaggg aaatgttttt tctaccctcg
       61 cttttgtttg ttcccgcctt tgtttttttt gcataggaga tttgttctac gatgctgtgg
      121 ctgaggaggg ctattgcgct tagtattaaa ataatatacg agagtccttc catccatttt
      181 gaaatggtat ctgctcgtgc agaaatagat cctgttatgc cgtttaaaaa caatataaga
      241 acgatggcgc ttccggcatg tagggcagat aagcttagag caactgcgag gggttcccac
      301 cacgctgctt tacgagaaat gtagagtgaa aagacaatgg tttttctatg tccggggccg
      361 agggcgtgaa agagaccaaa ggcaaaggca gcagcaagga tggtccaaaa aatgctctct
      421 gctttttttt gatcggctgt attccctgac tcttgcaaag ccttccattc ataaaaggag
      481 ttccctaaag tttctcgaag ccctcgttgc acttccaagt ttttttcact tgtttgcccg
      541 agccgtattt gtggggttgt ttttggtttg ccttcttctt tttcgacttg ctcttgatgt
      601 gcattttcaa actgcaaaga ttccctttct gtttgctccg tctcttcggt cggggagctt
      661 gtgcttaaaa aaggatttgc gtgaagccct gtgaaaaaaa agaaagcgag gatgaagatg
      721 actttatttt tttgaagcgc tctcatagcg aatcctatca gtatgaaatt tttacttcaa
      781 tcggataata ggtttgtaag cctttttccc acttgtagta aacccgtgta tcatcaattg
      841 ctccaagtgg attataataa actggatact ctttatttgc cgaaatcgaa tactgcggtt
      901 ttatactgct gttttttaat gtgaactcaa tgccagcact ttctggataa cttacatcgg
      961 taaaaaaagt atagtcatag tttgcaaaat agagctcgcc ctttggatac tgtgaaaggt
     1021 caatataaaa tcgatatatc atttgaccct tttctatgcg tgctctaaac tgctcaacct
     1081 ttgctggatt tgttcgctca cttccctgac gaataaaggt atagtaataa taatgccgta
     1141 aattgataaa ggcattgtta tagacttcta cactttcttt ttcatcaaac tgcccgtttt
     1201 tatcgacatc gagccaggag ataatatcgg cactaaaaaa gtgatcaaac ttccactcta
     1261 cgtagacgcc ttctaacttg ttatcttcaa aaacaaaatt cagtttgctt tcaattgcaa
     1321 cgtggggatg cgcaaaaaga ggcgtgaggg aaaggaggat aaagagaaaa agagaaaaat
     1381 atattttttt gtaagctctt aatttcaatt gcatgtttgt cagtatatca gttttatttc
     1441 tttttatcta gcgcgtgctt ttttcttcca tagatgagtc gtaatctaag tgcgtttgat
     1501 acaaccgata tggagctcat tgccatcgca aagcctgcaa acatggggct tagtaaaagt
     1561 ccaaatctag gatagaggag tccagctgca agggggagtc cgattgtatt gtataaaaat
     1621 gcccaaaaga ggttttgttt tataacgcgt aaggtgaggc gactgaggac gagtgcatct
     1681 tttatagaag atatatttga attcatcaat ataatatccc ctgcttcaat ggcggtgtcg
     1741 cttccctttc ccattgctgc ccctatgtca gcttgaacaa gggcggggga gtcgtttatg
     1801 ccatctcctg ccatgagaac tatttcgcct ttttcttgta aatcttttat cacctctgcc
     1861 ttgtcttcgg gtagcacttc ggctatcacc tgatctgctc ctacgaggga ggcaacatag
     1921 gcagcacttt ttttattatc tccactgagg aggataattt ttttacccat ttttttaagg
     1981 gcctgtattg cctgtatgga atcttctttt atttcatctt cgattgcaat gagggcaaga
     2041 agctttgtgt cgattgcaag atacatgaga cttgcacctt cgtcttgttg tttttcagct
     2101 tcttctgcaa gggcatcgac tgctattttt tctgtgttca tgaggcgctc gtttccaacg
     2161 aggaggctga gccttttatt ttcttggatg aggtttgcag agagtccaaa gccagagagg
     2221 tttgtttcac tttctagcaa gatattcatt tgagtcaatg tgttttcatc aatgcctctt
     2281 ttttttgctt cttctacaat cgcatgggca ataggatgac tgctgttttt ttctattgca
     2341 taggcaaggg caagaattat tttttcttca tcgatactat cttgcttcgg atttatttta
     2401 tcttgataaa aacgcaaact ctcttgagca tcctttgtta ggctaataga ttttactacg
     2461 ggttttcctt tggtgattgt ccccgtttta tcgagtacaa cggctgttgt tttttttagc
     2521 agttcgagca cttcgggagt tcgaattaaa atgccgtttg aggcagcaag tcctgttcca
     2581 accataattg ctgtgggggt tgcaagcccg agagcgcaag gacaggcgat aaccaaaacc
     2641 gaggttacaa cacgaataac aaaatgcgct tcagcaccgc ttagaatcca tatgatggcc
     2701 gagacgaggg ctatagcaag gacgacgggt acaaagatac cagagacaat gtcagcaagg
     2761 cgagcgatgg gcgctttttt gccttgtgtt tcttctataa aagagataat ttttgcaagc
     2821 gtggtatcct ttcccgttct cgtaacccgc ataaaaagta caccgcttgt attgagcgta
     2881 cctccgatga ggctgtcgcc ttcttccttg gaaacaggca tactctcgcc ggtaatcatt
     2941 gcttcattga catcgctttc tccttcgagg acaatgccgt caagaggaat tgtttcaccc
     3001 ggcttgataa gaagcacatt tcccacaacg atttcttcta gctggacagc ccttgggggc
     3061 agcataagac ttgggtcgtc atagcctgct acgagcgtag cttttggggg agagagtttt
     3121 ataagagagc taatcgccga tgttgtttta tcggtacttt ttgcttctaa aaatttcccc
     3181 gtcatgataa aggtgagaac agaggctgca gattcgtagt agaggttgtg gacatggctt
     3241 gcatcgaggg agatgagaaa tgtaagatag agcgaataca caaatgacgc aagcgagccg
     3301 attgcgatga ggctgtccat gtttggggct ctgtagtaga gagatttaaa accacgataa
     3361 aaatacgcgc gggcaaaata caagactggt atggtgagta agagttgcag aactgcaaag
     3421 ttcataggaa gcgcatgcat cgaaaaaatg gaaaacagtg gaagcggctt cgcaagcatt
     3481 tgtcccatgg aaacatagat gaggacaatc gtaaaaagcc atgcaccaaa aagctctttt
     3541 ttgagctgct ttacttttat agaagcatct tcgagagacg cgtttacttg agagctattt
     3601 ttttccattg gtttttgttc agcttcaaga cttgcagaat agcccgcctt cgtaaccgcc
     3661 tttataattt cgtcctttga aatactttgc ccagagtcaa attcaacaga gagctttttt
     3721 gtcgtgagat ttacatttgc ttcttttata ccttcaagct ttcgaacaga cctttcaaca
     3781 gcgctactac aagctgcgca actcataccc tcaacagtaa atgtttcttt catttttgcc
     3841 tccatcaaac tcctactata ataatcatac ggatatacta ggaataagtc aagttctaag
     3901 acttgtgaag cgatattttt actggaggcc tgttattttt ttaataaagt cttgtaatct
     3961 atgtggtgtt cgcgtacata gctaataaat tctttttctt cttcggttac tctcatttga
     4021 tattttaaag cttgtttgta tttttgtttt ctgccagcat tttttctgac tcccccgtgg
     4081 gtaaatgttt tttcataaaa gctaagacca gatttttttt tgtattctgt ttcgcttatt
     4141 attgtatcat tttgttgtag ttcagttttt agtttttttt gcacatagcc ttctgaatct
     4201 ttaatataca cgtattccat atttagacct cttttaatct ttttttagca agcttaataa
     4261 tttcttttgg cgtttttttt gttttcttta caaaaacgac gcctataata attatttgtc
     4321 cggaacaata tttaaataaa gatcgtattt ctttactttt tatttcaaaa atatttcctt
     4381 gtaagtgctt tattcgtaca tattcaatgc cttgtgtttc aattaaggtg taatctttgc
     4441 tcaggacatc ctgttgtttt tcttctagtt tagagatttc aaattgggca aagttttgta
     4501 ttttgatttt aaatatcatt ataagcataa taagtcaatc ttttgatttt gtcaacatat
     4561 tcaagatatt tttatagtat ttctccactc cagactttat ctaaaaaaag atgccagggc
     4621 atgtgttcta taccattttc gttttattgt ttttctaaaa acgggcaact caaaggcaag
     4681 gagggtatca tataaaattt caaaccagag gcgcactgtt tggcgtggaa tttgggcatc
     4741 atttgcaata ttcgtataat taataatgcc agtatttgat agggctgcga cttccaaaaa
     4801 gcgtgcaaag ccgcttaaac ttctggaaag tccttccgca gctatttctt ctttgaggta
     4861 ggtgttaaca tagtcggata aatcttcttc gactgtactg gcttctgatg ttagaaaaaa
     4921 gggcggcagt aaacctgttg ttaaaatatg ttcaagtgaa aaaagtggat gatttttaat
     4981 ttcgggatac acaaaaggat gtattttccc ataaaaactc cttttgccaa caaatcgcca
     5041 actctcttgg cgtttttaca atacccgagc tatagggttt tgtcaagatt ttttttgaat
     5101 gcgagtggaa atttgctgag aagaattcaa gcattcaatt ctgttg
//
LOCUS       DAOSSW010000101         4932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_15535_length_4932_cov_4.820381, whole genome shotgun sequence.
ACCESSION   DAOSSW010000101 DAOSSW010000000
VERSION     DAOSSW010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15535_length_4932_cov_4.820381"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /gene="asd"
                     /locus_tag="PKW26_07540"
     CDS             <1..981
                     /gene="asd"
                     /locus_tag="PKW26_07540"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOQ93456.1"
                     /translation="AGKTYKEAVSGRWLIGSDIPEGVKNLMVYDASDVDAAVGKCAFV
                     FSALEMGKDEIKALEAAYAAKDIPVVSNASANRWTDDVPMLIPEINPDHVEIIHRQKA
                     HHGWNKGFIAVKPNCSLQSYLAPVHALQEKGYPVKRLIVTTMQATSGAGYPGVSSFDM
                     IDNIVPYIGGEEEKTETELLKILGRVSGEKIENASGPLVSAHCNRVPVIDGHTACVSL
                     EFDLPEDKKPSLEEIEAIWTSFSGFPQEAKLPSAPEHAIVLRHEDNRPQPRRDRENGK
                     GMAVTIGRLRKCHVFDIRFVALSHNTKRGAAMGGILNAELLKAKGFFDEL"
     gene            1131..3554
                     /locus_tag="PKW26_07545"
     CDS             1131..3554
                     /locus_tag="PKW26_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOQ93457.1"
                     /translation="MQYGYFDDENREYIITNPRTPVKWVNYIGTLDFGGFLDHTGGLQ
                     LCKGDPALNRITKYIPQLPSSDFKGETAYLRYRKPGDTAKEANLISPYWLPTFNDYDL
                     FQCHVGLYYNRWKTEISGFSFDILAFIPQGGTEVIRQYKITNNTSEVQQLEFFPVVEY
                     SHFDALKQLTNADWVPQTMQSHKIDFEDGRGAIASYAFMKRDEAVNVLYANRPAIAWE
                     TDRKRFLGDNEYGTWREPLALKEERLSCKNAVRGDTIAALQLDLGLLQPGDSVSFITL
                     LTQGESLESIPRICKKYMSEADVSAAFSELSAFWDRYLSVFKVETPSEAFNSMINVHN
                     PRQCYTTKMWSRYMSLYQLGYGSDRGIGFRDSSQDTMGVIAQIPHEARELMEKLLSVQ
                     SQDGSAYHQFNPLSMEAGRGDSLEYEDRPHYYSDDQLWIVLAVIAYLKETGDYAFLDK
                     TIPFYEKDREGQPIEKGTVYEHLQRAVNFTKQNTGKHGLPLLGFADWNDTVNLPTGAE
                     SVFTANLYGWALKELSALADFRGEKKHKEKYDSWYDEMKKTVLEKAWDGEWFVRYFDE
                     KGEAIGSNKNEYGKLWLNAQSWALLSGFASDSDKGRRAMEAVQTHLATEKGIKLSWPG
                     YNGYDREKGGVTSYPPGAKENGGIFLHPNPWAIMAETMLGNGDRAFSYYNNINPAAKN
                     DIMDEYECEPYCYAQNILSDEHPQFGLGRNSWLSGTASWMYQSSTQYILGLRPEHEGL
                     LFDPCIPRDWKEFKIERKCRGAIYDIRVENPLGKSKGVRSILVDGKPITGNLIPYFTE
                     GRHSIEVSM"
     gene            3642..3833
                     /locus_tag="PKW26_07550"
     CDS             3642..3833
                     /locus_tag="PKW26_07550"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOQ93458.1"
                     /translation="MVFPLQELVEYKGNIYEITCAASRRAFQLSKINDESLEENEGKV
                     VSLAARQLFTNEVEYRIEE"
     gene            3811..4824
                     /gene="miaA"
                     /locus_tag="PKW26_07555"
     CDS             3811..4824
                     /gene="miaA"
                     /locus_tag="PKW26_07555"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOQ93459.1"
                     /translation="MSIESKNKEYSSASQNFIHSKIPVLLICAATATGKTDFLLSSFN
                     KTDHEIISADSLQVYKELSIGTAKPTLAERSCLPHHLIDIIEPKKEFGSGDFVRLADH
                     AVLDIWNRGKKPIIAGGTGFFIKNFLYGLPPTPAVDEAIRSDLQKRLKNEGAEALYEE
                     LLSLDAERAKKLHPNDSYRVLRALEVYYTSGKKLSTFALAPQLRDNYAFLVLYLTRER
                     DSLYERINERVDRMFEKGLWEELVALTTGRKNSLVESDPSMSAIGYKEFFLHSNNSGK
                     SIADFNAEDCLAVKSQIQRNTRQYAKRQIVFFKSLSPAISLHIDDNLAIKTIINDFYA
                     SFT"
BASE COUNT         1562 a          936 c         1069 g         1365 t
ORIGIN      
        1 gcaggaaaaa cctataaaga agcagtttcg gggcgttggc ttatcggctc cgatattccc
       61 gaaggcgtga aaaacctcat ggtctatgac gcaagcgatg tcgacgcagc tgtaggaaaa
      121 tgtgcttttg tctttagcgc acttgaaatg ggtaaggacg aaataaaggc attggaagca
      181 gcctatgcag caaaagacat tcctgttgtt tcaaacgcct ctgcgaatcg ctggacggat
      241 gacgtcccta tgcttattcc agaaattaac cccgaccacg ttgaaattat tcatagacaa
      301 aaagcacatc acggctggaa taaaggcttt atagctgtta aaccgaattg ttcactgcag
      361 tcttatttag cacctgttca tgccctgcaa gaaaaaggct atcccgtaaa acgcttaatc
      421 gttaccacaa tgcaagcgac aagcggtgca ggctatcctg gcgtttctag ctttgacatg
      481 attgacaata ttgtacccta cattggtgga gaagaagaaa aaacagaaac tgaacttctg
      541 aaaattcttg gaagggtctc tggcgagaaa atcgaaaatg cctctggtcc ccttgtttct
      601 gcccactgta atcgtgtacc agtaatcgat ggacatacag cctgtgtgag tttagaattt
      661 gatttacccg aagataaaaa accaagtctt gaagaaatag aagcaatatg gacttctttt
      721 tcaggctttc cacaagaagc aaaactcccc tccgcacctg agcacgcaat tgttctgcga
      781 catgaggata accgaccgca gcctcgtcga gaccgtgaaa acggcaaggg tatggctgtt
      841 acaatcggac gccttcgaaa atgtcatgtc tttgatattc gctttgttgc cttgagccat
      901 aacacaaaac gtggtgctgc catgggtggt atcctaaatg cggaattgct caaagcaaaa
      961 ggtttttttg acgaactatg agaaattaaa ctttgctgat atacgaaaga gtattttacc
     1021 ttatgataga ggtgaggtac tctttttttt agcttgacga acctaaaaaa ataactatac
     1081 ttgggttagt tggatacaga aacttacgtg atatcttttt gggaggcaat atgcaatacg
     1141 gttattttga tgatgagaat cgagagtata ttattaccaa tccacgaact cctgtaaaat
     1201 gggtgaatta tattggaact cttgattttg gtggcttttt agatcatacc ggtggtttac
     1261 aattatgcaa gggggatcct gcattaaacc gcatcacaaa atatattccg caattacctt
     1321 ctagcgattt taaaggcgaa actgcgtatt tgcgataccg aaaaccgggg gataccgcaa
     1381 aagaagcgaa cttaatttcg ccgtattggt tacccacctt taatgattat gatttatttc
     1441 aatgccatgt aggcttatat tacaatcgat ggaaaactga aatttctggt tttagttttg
     1501 atattctagc ctttattcca caagggggta ctgaagtcat tcgccaatac aagattacga
     1561 acaatacttc agaagtccaa caactagaat tttttccagt ggtagaatat agccattttg
     1621 atgctttaaa acaattaaca aacgcagatt gggtgccaca aactatgcaa tcacataaaa
     1681 tcgattttga agatggtaga ggagcaatag catcgtatgc ttttatgaaa cgcgatgagg
     1741 cggtaaacgt tttgtatgca aatcgaccag caattgcatg ggaaacagat cgaaaacgct
     1801 ttctaggtga taatgaatat ggtacatggc gggaaccact cgctctaaaa gaagagcgac
     1861 tttcttgtaa aaatgcagtt cgtggcgata ccatagccgc actacaactc gatttagggc
     1921 ttttacagcc aggtgattca gtttctttta taactctgct cacgcaagga gaatccttag
     1981 agagcattcc acgtatctgc aaaaaataca tgagcgaagc agatgtttca gcagccttct
     2041 ccgaactttc tgccttttgg gatagatatt tatccgtttt caaagttgaa acaccatctg
     2101 aagcatttaa ttcaatgata aatgtacata atccacgaca atgttatact accaagatgt
     2161 ggtctcgtta tatgtcccta tatcaacttg gatatggctc tgatcgaggc attggtttta
     2221 gagattcttc ccaggatacc atgggggtta tcgcacaaat cccccacgaa gcacgcgagt
     2281 tgatggaaaa attgctttcc gtacaaagtc aagatggatc ggcctaccat cagtttaatc
     2341 ctttgagtat ggaagcagga cgtggtgatt cgctagaata cgaagatcgc cctcactatt
     2401 attcggatga tcagttatgg attgtccttg cggtaatagc ttacttaaag gaaacaggag
     2461 actatgcctt tctagataaa accattcctt tttatgagaa ggatagagaa gggcagccaa
     2521 tagaaaaggg aactgtgtat gaacacttac aacgagctgt gaattttacc aaacagaata
     2581 caggaaagca cggtctgccc ttgcttggtt ttgctgactg gaatgatacg gtgaaccttc
     2641 cgacaggtgc agaatctgtt tttaccgcaa acttgtatgg atgggcattg aaagaacttt
     2701 cagcacttgc cgacttccgg ggcgaaaaaa agcataaaga aaaatacgat agctggtatg
     2761 atgagatgaa gaaaaccgta cttgaaaaag cttgggacgg cgaatggttt gtacgctatt
     2821 ttgacgaaaa aggtgaggct ataggttcca ataaaaacga atacggaaaa ctttggctca
     2881 atgcacaatc atgggcactc ttatcaggct ttgcaagcga ctcggataaa ggaagacgtg
     2941 ctatggaagc tgttcaaacg catcttgcaa ccgagaaggg aataaaacta tcttggccag
     3001 gctataatgg ctatgatcga gaaaaaggtg gtgtaacgag ctatcctcca ggagcaaaag
     3061 aaaatggcgg tatcttttta catccaaatc cttgggcaat tatggcagaa acgatgcttg
     3121 gtaatggaga tagggcattt tcttactata acaatataaa tccagcagca aaaaatgaca
     3181 taatggatga atatgagtgc gaaccctact gctatgcaca aaatattttg tctgatgagc
     3241 acccacagtt tggtttggga agaaattcct ggctttcggg aactgcaagt tggatgtatc
     3301 aatcttcaac acagtatatt ttgggtttgc gtcctgaaca cgagggcttg ctttttgatc
     3361 cctgtattcc gcgcgactgg aaagagttta aaattgaacg aaaatgccgc ggcgctatat
     3421 acgatattcg cgtagaaaat cctttgggta aatcaaaagg cgtacgctct atccttgtcg
     3481 atggtaagcc gattacgggt aatcttattc cgtattttac agaaggtagg catagtatag
     3541 aagttagtat gtaaaagaag gcgacgtcaa gagccttgtg ccttgacgtc gctttgttta
     3601 tgctatataa tactatttca atacgcgtat ttggagtaaa aatggttttt cctttgcaag
     3661 aattagttga atataagggt aatatttatg aaattacctg tgccgctagc agaagggctt
     3721 ttcagctttc aaaaataaat gatgaaagtc ttgaagaaaa cgaaggcaag gtggtatctc
     3781 tcgctgctcg ccagcttttt acaaacgaag ttgagtatcg aatcgaagaa taaagaatat
     3841 tcttcagcat cacaaaattt tatacactca aaaatccccg ttcttcttat ctgtgcagca
     3901 accgcaacag gaaagacaga ttttctttta tctagcttca ataaaacaga tcacgaaata
     3961 atctcagccg attccttaca agtgtacaag gaattatcta ttgggacagc aaagccgacg
     4021 cttgcagaaa gaagctgcct accacatcat ctcatagata taatcgagcc aaaaaaagag
     4081 tttggcagtg gagactttgt gcgtcttgct gaccacgcgg ttttggatat ttggaataga
     4141 ggtaaaaaac ccataatcgc aggtggaaca ggttttttta taaaaaattt tttatatggg
     4201 cttcccccaa caccagctgt tgacgaggct attcggagcg atttgcaaaa gcgtttaaaa
     4261 aatgaagggg cagaagcttt atacgaagag cttctgtcgc ttgacgcaga gcgggcaaaa
     4321 aaactacatc caaatgactc gtatagggtt ttgcgcgccc tcgaagtcta ttatacaagt
     4381 ggcaaaaaac tctcgacctt tgcccttgca cctcagcttc gcgataacta tgcattttta
     4441 gttttgtatc taacacggga gagggatagt ttatacgagc gcataaatga aagagtagat
     4501 agaatgtttg aaaaaggact ctgggaagag cttgtcgcac ttacgacagg gagaaaaaac
     4561 tccttggtcg aaagtgatcc gagcatgagt gctatcggct ataaggagtt tttccttcat
     4621 agcaataatt caggcaaatc gattgctgac tttaacgccg aggactgtct tgcggtaaaa
     4681 agccaaatac aaagaaacac acggcagtat gcaaaacggc aaatagtttt ttttaaatcg
     4741 ctttcacctg caatttctct tcatattgat gataatttgg ctataaaaac aataattaat
     4801 gatttttatg cttcttttac ttgacgtaaa ctaaattatt tatcataata gtagaacata
     4861 gataaaggag ttttgttcaa tgccttgtgg aaaaaagcga aaacgcaaaa aaatagcaac
     4921 tcataaacga aa
//
LOCUS       DAOSSW010000102         4785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_16168_length_4785_cov_4.639746, whole genome shotgun sequence.
ACCESSION   DAOSSW010000102 DAOSSW010000000
VERSION     DAOSSW010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16168_length_4785_cov_4.639746"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /locus_tag="PKW26_07560"
     CDS             complement(<1..1487)
                     /locus_tag="PKW26_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOQ93460.1"
                     /translation="MKKCISRPKALSIGMVLLTILFSNLVLSSTQIFAQTASENERET
                     ALRSRQYIELIGSIFSYVENNYVDKLNPELLYEGALKGMLEALNDPYTSYLDKSNMRD
                     LNDTTVGNFGGVGLTISKPVASSPEKPAYVEVSSPIDGGPGFKAGIQAGDFLVEINGI
                     ETKDITMEEVLSMLRGTIGETVEVLVKRGKTMQFSVKITRELIEVPTVKYDMLKTKNK
                     NLGYLKIIEFTPLTAQRVQEALDIFYDNQYEGLIIDLRNNPGGLIDSVYQVADKFIDA
                     GPIVSTRGRQSFNDTTFFAAARNTAVRNMPIVVLINRGSASASEILAGALKDDHLAYL
                     VGERTYGKGSVQQVIPLYNKDGIKLTIARYYTPSDVNIDKIGIPPDFEVLFPELTEKE
                     ETAYVDLLESKHIEEYVQEHPNMNENDIAAYAKLLKKNYPLNEESLRKLIRNEVYRTR
                     GSLLYDLDYDVQLRAAIEIFESNSYDERMKEAKTLKELQDAFVLA"
     gene            1580..1996
                     /locus_tag="PKW26_07565"
     CDS             1580..1996
                     /locus_tag="PKW26_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93461.1"
                     /translation="MRKVQVLCILSSLYEIAKLIVIIFSNYTVHTVALPYSWYSASPL
                     LILPLFFCLFIFYKPLVYKTYIFPYILTKIMALMSSFFFIYHLTISKSPELMQENFRF
                     LQQFILFLLLFFIIDAILLVCFILVDRKLKVEVKTE"
     gene            2013..3143
                     /locus_tag="PKW26_07570"
     CDS             2013..3143
                     /locus_tag="PKW26_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002677971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOQ93462.1"
                     /translation="MQIIPVASGKGGVGKSLVAANLAIALGKAGKKVIIADLDLGASN
                     LHLVLGQQAPKAGLGTYLSGGIDFSAIILDTNYENVQFIPGDAEIPGLTAMKISQKND
                     IIKKLQNLDTDFLIVDLGAGTHTSILDFFLLSPQGIIVTSPTVTATLDAYLFLKNAVF
                     RLMYNSFKKNSKAYKFLESLKSDAHSLQRIYIPKLIDTLMQVDPQSTEVFRRRIARFK
                     PRLILNMIDDPKDADKAQKIRRSCTEYLGLELEHLGIIYRDSLQDIALASRLPIVIYK
                     PNAMLSQAIFRISEKIAFSETIRFDSDGNYDSFQYAESEAQEDFENKMTYIEELLGTG
                     ALTMNELAETIKTQQYEISQLKKENALLKTKIVKALSQGFTL"
     gene            3153..4127
                     /gene="lgt"
                     /locus_tag="PKW26_07575"
     CDS             3153..4127
                     /gene="lgt"
                     /locus_tag="PKW26_07575"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOQ93463.1"
                     /translation="MLLAINYPSWIHPQIFPNIPFLGLLRWYGLMYVFAFATAYLVMN
                     KLRKNKKLDTNNVETSEDDLFSFFLTGIIFLLIGARLFSALIYDSSKLYYRKPWLIFW
                     PFDENWNFTGLAGMSYHGGVVGGTLGIVLWCIRHKKPVLQWADAMAVAIPLGYTFGRL
                     GNFLNAELYGRITTMPWGMIFPDAQRFSASIPWVAEFAQKAGLEISSGLVNLPRHPSQ
                     LYEAFFEGLFLFGVNYAVSKKKPYPGFLTGLFYAGYGFVRFIIEYFREPDIDLGFRIA
                     KISDTPTYLNVSLLNISTGQILCFIMFVAGISLIIVQGIRHKKMKSSD"
     gene            complement(4136..>4785)
                     /locus_tag="PKW26_07580"
     CDS             complement(4136..>4785)
                     /locus_tag="PKW26_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOQ93464.1"
                     /translation="RLSALMKLPVLYIFTHDSIFVGEDGPTHQPIETLAALRAIPNMR
                     LLRPGDAEESVEAWKIAMNHKTGPSCLILSRQNIQVYEKADPDWKESIKMGAYVVKNT
                     ETEPDITILATGSEVSLALDALEISKNKNVRVLSVLDRELFLAQDALLQETLIGRPRR
                     LITVEAGSKMGWEGLASSKEDAFHLDSFGESAPGSVVAEHFSFTKEALASCIDRD"
BASE COUNT         1414 a         1009 c          848 g         1514 t
ORIGIN      
        1 tcagctaaaa caaaggcatc ttgtagttct ttaagtgttt ttgcctcttt cattctctcg
       61 tcataggagt ttgactcaaa tatttctatt gccgcccgta actgcacatc gtagtcaagg
      121 tcgtagagta aagaaccgcg cgttctgtat acctcattgc gaataagttt acgcaagctc
      181 tcttcattta ggggataatt ttttttcaaa agttttgcat atgcagcgat atcgttttca
      241 ttcatatttg gatgttcttg cacgtactct tctatatgtt tcgattcaag taaatccaca
      301 taagctgttt cttctttttc tgtaagttca ggaaacaaaa cctcaaaatc tggcggaatt
      361 cctattttat cgatatttac atctgatggt gtgtaatacc gtgctatggt caactttata
      421 ccatctttgt tgtacagcgg tataacttgt tgaacagaac ccttgccata tgttcgctca
      481 ccaactaagt aggcaagatg gtcgtctttc aaagcgcctg ccaaaatttc tgaagctgag
      541 gcagaacctc tatttataag cacaacaatg ggcatattgc gcacagcagt gttccttgct
      601 gctgcaaaaa aagtagtatc attaaaactt tggcgtcccc gtgtcgacac aataggacct
      661 gcatctataa acttatctgc cacttgatag acgctatcga tgagcccgcc gggattattt
      721 cgtaagtcga taattaaacc ttcgtactga ttgtcgtaga aaatgtcaag cgcttcctgt
      781 actctttgtg ccgtcaatgg agtaaattca ataattttta agtagccaag gtttttgttt
      841 ttggttttta acatatcgta tttaaccgta ggtacttcaa taagttcccg tgtaatcttt
      901 actgaaaact gcatagtctt tccacgtttt acgagaacct caactgtttc tccaatcgtt
      961 cctcgaagca tagaaaggac ctcttccata gtaatgtcct ttgtttctat gccgtttatc
     1021 tctacaagga agtcgcccgc ttgaatccct gccttaaaac caggaccacc atcaatgggg
     1081 gaggaaactt caacataggc tggtttttca ggacttgaag cgactggctt ggaaatagtt
     1141 aaaccaacac ctccaaaatt tcccacagta gtatcgttta agtcacgcat attcgattta
     1201 tcaagatagc ttgtatatgg gtcatttagg gcttcaagca tgcccttcag agcaccttca
     1261 tacagcagtt cgggattgag cttgtcaaca tagttatttt caacataact aaaaatacta
     1321 cctatgagtt caatatactg acgagaacgc aaagcagttt ctctttcatt ttcagaggct
     1381 gtttgagcaa atatctgagt acttgataaa acaagattag aaaataaaat agttaaaagc
     1441 accatgccta tactcagtgc cttgggtctt gatatacact ttttcatttt ttcattgtaa
     1501 ttcgtttaca tttttttatc aactataaaa acagaaaaaa aggcttgacc tttctcctta
     1561 gatttgtaga ctacagtata tgagaaaagt tcaagtttta tgcatcctct catcactcta
     1621 cgaaatagca aaactcattg ttattatctt ttcaaattat actgtccaca cagttgctct
     1681 accctattct tggtatagtg caagcccact tctcatttta ccgctgtttt tttgtctctt
     1741 tattttttat aaaccacttg tatataaaac ctatatattt ccatatattt taacaaaaat
     1801 aatggctctt atgagctctt ttttctttat ctatcacctc actatttcaa aaagtccaga
     1861 gcttatgcaa gaaaatttta gatttcttca gcaatttata ctctttttac tgcttttttt
     1921 cattatcgat gctatacttt tagtatgttt tattttagtc gatagaaaac taaaagtcga
     1981 agtaaaaacc gaataaaaat ggatgggcta atatgcaaat aatccccgta gcaagtggaa
     2041 aaggtggcgt tggaaaaagc ttggttgcag caaacctggc aattgcctta ggaaaagctg
     2101 ggaaaaaagt tattatcgct gatttagatt taggtgcctc aaacctacac ctcgtcttag
     2161 ggcaacaggc accaaaagca ggtctaggaa cctatctcag cggcggtatc gatttttctg
     2221 ctataatctt ggataccaat tatgaaaatg tgcaattcat tcccggagat gcagaaatcc
     2281 ccggcttaac cgccatgaag atttcacaga aaaacgatat cataaaaaaa ttgcaaaacc
     2341 ttgataccga ttttttaatc gtcgatttgg gagctggtac acatacatcc atcctcgatt
     2401 tttttctcct ttctccacag ggaattatcg taacaagccc aacggtaacc gctaccctcg
     2461 atgcctattt gtttttaaaa aatgcagtat ttcgcctcat gtataattcc tttaagaaaa
     2521 attctaaagc atacaaattt ttagaaagcc taaaatctga tgcacatagt ttacaacgaa
     2581 tctatatccc caaattaatc gacacgctta tgcaagtaga tccacaaagc actgaagttt
     2641 ttagacgtcg catcgctcga tttaaaccac gccttattct caacatgatt gacgacccaa
     2701 aagatgcaga taaggcacaa aaaatccgtc gctcttgcac agaatatctt gggcttgaac
     2761 tcgaacatct gggaattatt tacagggata gtttgcaaga tatagctctt gcatctcgct
     2821 tacctatcgt tatctataag ccaaatgcca tgctctctca agcaattttt cgcataagtg
     2881 aaaaaatcgc attttcagaa acaattcgtt ttgattccga tgggaattat gacagttttc
     2941 aatatgccga aagtgaggct caagaagact ttgaaaataa aatgacctat atagaagaat
     3001 tgcttggcac aggcgcttta acgatgaatg agcttgccga aactataaaa acacaacaat
     3061 atgaaataag tcagttaaaa aaagagaatg cgcttttaaa aacaaaaatt gtaaaagccc
     3121 tatctcaagg atttacacta taagaggtta ctatgttatt agctattaat tatccttcat
     3181 ggatacatcc acaaatattt cccaacattc cattccttgg tttattgcga tggtatgggc
     3241 tcatgtacgt ctttgccttt gctaccgcat atctcgttat gaacaaactt cgaaaaaata
     3301 aaaagctcga tacaaacaat gttgaaacaa gcgaagacga tttgtttagc ttttttttaa
     3361 ccggcattat ttttctactc attggggcac gtcttttttc tgccctcatc tatgacagct
     3421 caaaactcta ttatagaaaa ccctggctta ttttttggcc ctttgatgaa aactggaact
     3481 ttaccggcct tgctggcatg tcctaccacg gcggtgttgt cggcggcacc ctcggcatcg
     3541 ttctctggtg catccgtcat aaaaaacctg ttttacaatg ggcagatgct atggctgttg
     3601 caatcccttt aggttacacc tttggacgtt taggcaactt cttaaacgca gagctctatg
     3661 gacgaattac aaccatgcct tggggtatga ttttccccga tgcacaacgg ttttcagcct
     3721 ctattccatg ggttgcagaa tttgcccaaa aggctggctt agaaataagc agcggtttag
     3781 taaacctccc acgtcatcca agccaactct acgaagcttt ttttgaaggt ctatttctct
     3841 ttggagtaaa ctacgcggta agcaaaaaaa agccctatcc aggttttcta acgggacttt
     3901 tttatgcagg ctatggtttt gttcgcttta ttattgaata tttccgagaa ccagatatag
     3961 acctcggttt tagaattgca aaaataagcg atacacccac atatttgaat gtatcgcttt
     4021 taaatatttc cactgggcaa attctttgct ttattatgtt tgtggcgggg atttcactta
     4081 taattgtcca aggaattcgc cacaaaaaaa tgaagtcgag cgattaagcc atcgattagt
     4141 ccctatcgat gcaggaagca agtgcctctt ttgtaaagga aaaatgttct gcaacaacag
     4201 agccaggtgc agattcccca aaggaatcga ggtgaaaagc gtcttctttg cttgaagcaa
     4261 gcccttccca gcccattttt gaaccagctt cgactgttat aaggcgtcgc ggtcttccta
     4321 taagcgtttc ttgcaaaaga gcatcttgcg ctaaaaacaa ttctctatca agtacagaaa
     4381 gtactctcac gtttttgttc ttgcttatct ctagcgcatc caaggcgaga gaaacttcag
     4441 agcccgttgc caagatagta atatcaggct ccgtttcagt gttttttacc acataggcgc
     4501 ccatttttat gctctctttc caatcaggat cggccttttc atacacctga atattctgcc
     4561 tcgataaaat caagcagctt ggacctgttt tatggttcat agcgattttc catgcttcaa
     4621 cactttcttc tgcatctcct gggcgaagca agcgcatatt tggtattgcg cgcaaagcag
     4681 caagtgtttc tatcggttga tgcgttggac catcttcccc aacaaagata gaatcatgag
     4741 taaaaatata gagcacagga agcttcatca aagcagaaag acgaa
//
LOCUS       DAOSSW010000103         4779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_16191_length_4779_cov_5.414056, whole genome shotgun sequence.
ACCESSION   DAOSSW010000103 DAOSSW010000000
VERSION     DAOSSW010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16191_length_4779_cov_5.414056"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1223)
                     /locus_tag="PKW26_07585"
     CDS             complement(171..1223)
                     /locus_tag="PKW26_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOQ93465.1"
                     /translation="MSFLQTEFWAKFKSQFGWTFSKHNFSSSFQQKEYTTELFVLHRR
                     FKGGFSLSYVPLAPILPQTCLEKYTSSDYFELVSTLVEDLRKNLPRKTLFIRFDFPPI
                     FPSLNEKDVFLQELLSYAKKSSFRLKKAPSDIQSPDTVVLDLKQDEETLLANMKAKWR
                     YNIRLAAKKGVEIAERGEKGLASFYTLYEETAKRDGIAIHSYAYYEALFRLAKSYPQL
                     SLRLYVAKHEDDILAAIITLFTKEEAVYLYGASSNKKRNLMSTYLLQWTAIKDAKIFG
                     SQSYDFYGIPPVEDETHPMYGLYRFKTGFGGEIIHRVGSVDIPLSPFYTFYVFAEKVR
                     YFWFKKIKKLFVKKAS"
     gene            complement(1224..3503)
                     /gene="metG"
                     /locus_tag="PKW26_07590"
     CDS             complement(1224..3503)
                     /gene="metG"
                     /locus_tag="PKW26_07590"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOQ93466.1"
                     /translation="MKTKQTKRRLITSALPYVNNVPHLGNLIQVLSADVFARFCRSYG
                     YDCLYICGTDEYGTATETKAAEEGISPKELCDRYHKIHKDIYDWFHIDFDHFGRTSAP
                     QQTEITQALFLDLDKNGFIVEKTIEQLFCTSCDRFLADRYVRGTCPHCGFDDARGDQC
                     EHCGNLLEPQELLNSRCLSCSNTPELRETKHLYIDLPKIQEHYGPWMKEASEKGQWAR
                     NALQMTQAWIRDGLKERAITRDLKWGIPVPKKGYENKVFYVWFDAPIGYISITKALTD
                     SLDDPEADWKAWWLPEESKIEKAKIPVELFQFIGKDNIPFHTLIFPSTLIGSGKNWTK
                     LFHMSSTEYLNYENGKFSKSKGVGVFGNDAKESGIPADAWRFYIFYNRPEKSDTQFTW
                     KDFQEKYNSELIGNLGNLVNRTLTFISRYYDGIIPEGEKDRELWEKILPIEEMITKKL
                     DWAELKEAFHGIFEISSIANKAFQDGEPWKTRETEPEKAKSLLFNLVYVIKDLMILAS
                     PFIPEYSKTVLSFLKAENLVWEDLGLLEGLDKVYDSKIIFKTLDNATIDSYRERYAGS
                     QKDRKEMESKIDTDKKTEEKAEDKQINQADLFNSSIALKIAKIVRIEKHPEADKLYIE
                     NLDDGSGEERVILSGLVPYYEAEELLGKHIVLADNLKPRKMRGIESRGMLLAADYKDA
                     EGNEKVEVLDCPWAAPGTPVILEGADPTIVKKESINADVFFSCKIEVKDSVVCIDGVP
                     LLVDGKKITTKYAKNAEVG"
     gene            complement(3524..>4779)
                     /locus_tag="PKW26_07595"
     CDS             complement(3524..>4779)
                     /locus_tag="PKW26_07595"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HOQ93467.1"
                     /translation="ESENIEITVPQAYLKTNTVFEKQYIYPATSSKFVPYDTKSINSI
                     REFLYEKPQAQSLYARMMYVNSQIMQYRGDSSRKKTAKQYLWQAQGQSAYFLLDDENI
                     KDYFNAKEEAYKHLLMAEKMVREAPDTSINEIVTSFDYDMDGKKEYLFLNTDFNAFIS
                     LSGGILYELDLLINNKNYCNTILRNKANDGVQDFYPKKMFIDHLIEEEEFKKYLADAS
                     QSSALFPLINYTETKFDIHKKELYLQAQVLFGLFQQPVSLRKIYAIKENGISVQYIIK
                     NEGPLQLKAKFAIESNLSITQLNDTQNIDLVILGNVNKIDCTKNCVHHNSVNLLKITD
                     PKSNNAFIFEPNEDSGISIQPLTISREFESKLNTFFQGINFSFFWDLNIAPGYEVEKM
                     LFLNIKPSKKNQTAKRKRKTLTKKS"
BASE COUNT         1368 a          869 c          884 g         1658 t
ORIGIN      
        1 tataccagca aaaaaagtaa tagttttaac agactgcacc tcacctgtaa aaggttttga
       61 ggatgttgga gcagcttttg ttgatgagta tagcgcaaaa ggaatgcttt ttaaagcttc
      121 taccgagatt cggttctagg agattttttt attttaggtc ataataacta ctaagaagct
      181 ttttttacaa agagcttctt tattttttta aaccaaaaat aacgtacttt ttctgcaaaa
      241 acataaaaag tatagaatgg ggaaagaggg atatctacac tgcccactcg atgtataatc
      301 tctccaccaa aacctgtttt aaatcgatac aagccataca tgggatgtgt ctcatcttcg
      361 acaggtggaa tcccataaaa atcatagctt tgcgagccaa atatttttgc atcttttata
      421 gcggtccatt gtaaaagata ggtactcatg agattgcgtt ttttatttga tgaggcccca
      481 tagagataga cagcttcttc ctttgtaaac aaagtgataa ttgcagcgag aatgtcatct
      541 tcatgttttg ccacatacaa acgtaaggaa agctgaggat atgacttcgc aagcctaaat
      601 aaggcttcgt agtaggcata tgaatgtatt gcaatgccat cacgcttagc agtttcttca
      661 tagagtgtat aaaaagatgc taagcctttt tctcctcgct cagcaatctc tacccctttt
      721 ttagcagcaa gtcgaatatt gtatcgccat tttgctttca tgtttgcaag aagtgtttct
      781 tcatcttgct ttaagtcaag aactaccgta tcaggacttt gtatatcgct cggtgctttt
      841 ttaagcctaa aggatgattt tttagcatag cttaaaagct cttgtaaaaa aacatctttt
      901 tcatttaaac tgggaaaaat aggaggaaaa tcaaaacgta taaaaagtgt ttttctcggt
      961 agatttttgc gcaggtcttc aactaaagta cttacaagct cgaaatagtc tgaacttgta
     1021 tatttttcta gacaggtttg aggcaaaatg ggagctaatg ggacataaga cagggaaaat
     1081 cctcccttaa accttctatg gagcacaaaa agctcagtcg tatattcttt ttgttgaaag
     1141 ctagatgaga aattatgttt agaaaaggtc cagccaaatt gggacttaaa ttttgcccaa
     1201 aattctgttt gtaaaaaaga catctagcct acttcagcat tttttgcata ttttgttgta
     1261 attttttttc catcgacaag aagtggtaca ccatcgatgc agactacaga atcctttact
     1321 tctatcttgc aggaaaaaaa tacatctgca tttatagatt cttttttgac aatggttgga
     1381 tcggctcctt cgaggattac aggagtccca ggtgccgccc aaggacagtc aaggacttct
     1441 accttctcgt ttccttctgc gtctttgtag tcggcggcaa ggagcattcc acggctttct
     1501 ataccgcgca tttttcgcgg tttaaggttg tcagcgagga caatgtgttt tccgagcaat
     1561 tcttcagcct cataataagg taccagtcca gaaagaataa ctcgttcttc tccagagcca
     1621 tcatcgagat tttctatgta gagcttatca gcctcgggat gtttttctat gcgtacaatt
     1681 tttgcaattt ttaaggctat gctcgagtta aataaatctg cttggttgat ttgtttgtct
     1741 tctgcttttt cttctgtttt tttgtctgta tctattttag attccatttc ttttctatcc
     1801 ttttgacttc ctgcataacg ctcacgatat gagtcaatag tagcgttatc gagggtttta
     1861 aaaattattt ttgaatcata taccttatcg agcccttcta aaagacctaa gtcttcccaa
     1921 acaagatttt ctgctttcag aaagctcaaa actgtttttg aatattctgg tataaaagga
     1981 gaagctaaaa tcataaggtc tttaatgaca tagacgagat taaataaaag agattttgct
     2041 ttttctggtt ctgtttctct tgttttccaa ggctctccat cttgaaaggc tttatttgct
     2101 atggaagaga tttcaaaaat gccatggaag gcttctttga gttcagccca gtcgagtttt
     2161 ttggtaatca tttcctcgat tggaaggatt ttttcccaaa gctctctgtc tttttctccc
     2221 tcgggtataa ttccgtcata atagcgcgag ataaaggtga gtgttctgtt tacgagatta
     2281 cccaaattcc ctataagttc gctattgtat ttttcttgga aatctttcca ggtaaactgt
     2341 gtatctgatt tttctgggcg gttataaaag atataaaaac gccaggcatc agcgggaatg
     2401 ccagattctt ttgcatcgtt tccaaaaaca ccgacacctt ttgacttaga aaatttaccg
     2461 ttttcatagt ttaggtattc ggtgctcgac atatgaaaaa gttttgtcca gttttttcca
     2521 gaaccgataa gcgttgaggg aaaaataagg gtatgaaagg ggatattatc tttgcctata
     2581 aactgaaaaa gttctactgg tatctttgct ttttctatct ttgattcctc tgggagccac
     2641 catgccttcc aatctgcttc aggatcatct aaggaatcgg tgagcgcctt tgtaatggaa
     2701 atatatccaa tgggagcatc aaaccaaaca taaaaaacct tgttttcata accttttttt
     2761 ggaacaggta tgccccactt aaggtctcgt gttatagccc gttctttaag gccatcacga
     2821 atccacgctt gggtcatttg taaggcattt cgcgcccatt gtcctttttc agatgcttct
     2881 ttcatccatg gaccgtagtg ttcttgaatt ttaggtaaat ctatatataa atgctttgtt
     2941 tcacgcaatt caggtgtatt tgaacagctg agacaacgac tattgagcaa ttcctgcggt
     3001 tccagtaaat tcccacaatg ctcacattgg tctccccgtg cgtcatcaaa gccacaatgc
     3061 ggacaagttc cacgtacata gcggtctgct aaaaaacgat cgcagcttgt acaaaaaagc
     3121 tgttcgattg tcttttcaac gataaaacca tttttatcaa ggtctaaaaa aagagcctgg
     3181 gtaatttctg tttgttgagg agccgatgtc cttccaaaat gatcaaaatc gatatgaaac
     3241 caatcgtaga tatctttgtg gattttgtgg tatcggtcgc agagttcttt gggacttatg
     3301 ccctcctctg cagcctttgt ttcagttgcg gtaccgtatt catctgtgcc acaaatatat
     3361 aagcaatcgt agccataact acggcaaaag cgtgcaaaga catcggcaga aagtacttgt
     3421 atgagattac ctaaatgggg aacattattg acatagggta aggcagatgt tataagcctt
     3481 cgttttgttt gctttgtttt catagttatc tattgtaatt tttttatgac ttctttgtca
     3541 atgtctttcg ctttctttta gcggtttgat tttttttaga aggcttaata ttgagaaaga
     3601 gcattttttc tacttcataa ccgggagcta tatttaagtc ccaaaaaaat gaaaagttta
     3661 taccctggaa aaaagtgtta agctttgatt caaattctcg tgaaatagtt aaaggttgaa
     3721 tacttatacc agagtcttcg ttgggctcaa aaataaaagc attgtttgat ttgggatcag
     3781 taatctttaa aagattcaca gaattatgat gtacacagtt ttttgtacaa tctatcttgt
     3841 taacattgcc taaaataaca aggtcaatat tctgtgtgtc atttaactgc gtaattgaaa
     3901 gatttgattc aatagcaaat tttgccttta actgaagcgg tccctcattt tttataatat
     3961 actgaacgct aataccattt tcttttattg cataaatttt tcttaagctt accggctgct
     4021 gaaacaagcc aaataaaacc tgcgcttgta aatagagttc ttttttatgt atatcaaact
     4081 ttgtttcggt ataattgatg agcgggaaca aagccgatga ctggctggca tcagctaagt
     4141 attttttaaa ttcctcttct tctatgagat ggtcgataaa catttttttg ggataaaagt
     4201 cttgtactcc gtcatttgcc ttatttctca agatagtatt gcaatagttt ttattattta
     4261 taagtaaatc aagctcgtat aggataccac cagagaggct tataaatgca ttaaagtcgg
     4321 tatttaaaaa gagatattct ttttttccat ccatatcgta atcaaaactc gttacaattt
     4381 cattgatact ggtatcgggc gcttcacgaa ccattttttc tgccatgagc aaatgcttat
     4441 aggcttcttc ttttgcatta aaatagtctt tgatgttttc gtcatcgagt aaaaaatacg
     4501 cactctgccc ctgtgcttgc cagagatatt gttttgcagt tttttttcgt gagctgtcac
     4561 cacgatattg cataatttgt gagttcacat acatcatacg agcataaaga ctttgcgctt
     4621 gtggtttttc atataaaaac tctcgtattg agttgataga ctttgtgtcg taggggacaa
     4681 actttgaaga agttgcagga tatatgtatt gtttttcaaa aacagtattt gttttaagat
     4741 aggcttgagg aacagtaatt tctatatttt cactttcgt
//
LOCUS       DAOSSW010000104         4684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_16622_length_4684_cov_5.976453, whole genome shotgun sequence.
ACCESSION   DAOSSW010000104 DAOSSW010000000
VERSION     DAOSSW010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16622_length_4684_cov_5.976453"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..735
                     /gene="rsxE"
                     /locus_tag="PKW26_07600"
     CDS             121..735
                     /gene="rsxE"
                     /locus_tag="PKW26_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HOQ93468.1"
                     /translation="MKKYWQLFKNGLVKENPLLFLMIGLCSSLAVTTSFENGLGMGLA
                     LTFVLLMSEVLISLLRKLIPDSIRIPVFIIIIATFVTIVELLMKAYMPDLAKALGVFI
                     PLIVVNCIIMGRVESFASKRTVAESAADALGMGFGYLWVLTGISIVRELFGNGTFIGM
                     TLIPEQYTIGFFTGAPGGFFVFAIFIAVTRLIRVAYEKNKGEQK"
     gene            732..1310
                     /locus_tag="PKW26_07605"
     CDS             732..1310
                     /locus_tag="PKW26_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HOQ93469.1"
                     /translation="MNEYLKIFLSSALIDNVVFIQFLALCPFIGMTADVHKSNGMGVA
                     TSFVMVLATVVTYPLYHFVLLPLDLAFLQTLVFILVIASLVQLVEYYLKKSAPALYSS
                     MGVYLALITTNCAILAVTLKSISKGYNFLESIVYSLGTAVGFLLAMVLMAGIRQKVKL
                     AKIPAFIKGTPVLFVAAGLLSMAFSGFSGLIK"
     gene            1321..2115
                     /locus_tag="PKW26_07610"
     CDS             1321..2115
                     /locus_tag="PKW26_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOQ93470.1"
                     /translation="MITVLYTLAVSATLALLLGFLLGFFKKIFFVEVDPKIMLIRDSL
                     PGANCGGCGYPGCDAFAEAVAAGEANPSGCTAGGKDTADKVCAIMGVSADALETVAVL
                     ACQGSKDHAVAKGNYTGVSTCRSALIGTNGIKVCEWACQGFGDCVTVCLFDAIHMGDD
                     GLPHVDETKCTGCGMCIIECPQKLLVHFNKTDKGAFARCNNRNKMIPQILKSCSVGCF
                     KCGKCVRACKYDAIHLVDGIPVVDYEKCTSCNDCVEACPTNVLYLR"
     gene            2219..3754
                     /locus_tag="PKW26_07615"
     CDS             2219..3754
                     /locus_tag="PKW26_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HOQ93471.1"
                     /translation="MAQPDNSGNFFQRLISAILGSFDPEAEKKRILRSIAKEVNKSKF
                     KFYKHSSGDAQVGLAKFFYEIYKNIGAAQVMFESTQNPNAFKHAVIDFVMNEKQRELI
                     EFLNEQAILALAQTMPPNELKKKIQTDLETLSQEFDIQKIQKIDALYTKLMLFQSFCT
                     FDFYFLLKKFDSSLMERDFNYMPRFEPIRGEYITDDLKDFIAVAWAMPLNEDWSEMLA
                     LLRNLKGIEPIAANTWNKTIQRLRQVQNSQILEKIIKLTTSDPSYQAFIPSRQERLFE
                     SYLDGLKKEVQETLVKIEKEVTNSRADNLLKNIFGTTAVIRLKYYSETANLNLEKKRV
                     PLFKYHQPLNYTKAFLLDYFKKNIREFSDLVLVRGKWVTAVLSGQMSEVYQNIAEISD
                     QITEFDEALSDEAAVGQKLKNLLLRVGRDREAERIIASLIKDTNDKALFYITSTAQNL
                     IALAKTIKSLLEDREKPEPQMLINWKELDKFVDHPIRDTGVEIYKMIHQFIQLIQIYI
                     PKK"
     gene            complement(3810..4607)
                     /locus_tag="PKW26_07620"
     CDS             complement(3810..4607)
                     /locus_tag="PKW26_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOQ93472.1"
                     /translation="MKRIILIVLVLSLVFGGVFAAGKKDDSVLKVGATPEPHAEMLNL
                     VAADLKAQGINLKVIEFTDYVTPNEALESGQIDANFFQHIPYMDSFNEEKGYHLVNAG
                     EIHIEPIALYSKKVASLADLPEKAVIAIPNDPTNEGRALLLLASAGLISLKDSSDLSA
                     TPLDIVANPKNFVFRELEAASLPRVLADVDAAVINGNYAIPAGLVASKDGLLVEGAKS
                     PYVNIITVKAGSESKANIKALVEALRSKKIADWVSERYPNGEVVVVF"
BASE COUNT         1447 a          984 c          871 g         1382 t
ORIGIN      
        1 tgccttaggg acaaacgtcg atgctatttc tggttcaacg attacctcac ggggaattgt
       61 caaaattctc aatgcagcaa tagaatctgg taaaaaagca ataacagagg gggctcagta
      121 atgaaaaaat attggcaact ttttaaaaat ggcttggtaa aagaaaaccc gctcctcttt
      181 ttaatgatag ggctttgttc ctcactagcc gttaccacaa gctttgaaaa cggacttggt
      241 atgggtctcg ctcttacatt tgtgctcctc atgagtgaag tgcttatttc tctactacga
      301 aaactcatcc ccgattctat acgaattcct gtattcatca tcatcatagc aacctttgta
      361 accattgttg agcttctcat gaaggcctat atgcccgacc tcgcaaaggc gcttggtgtt
      421 tttatccctc tcatcgttgt aaactgtatc atcatgggac gagttgaatc ctttgcttca
      481 aaacgaactg tggcagaatc tgccgccgat gccctcggca tgggttttgg ctatctttgg
      541 gttctcaccg gcatttccat cgtacgcgag cttttcggaa acggcacctt cataggcatg
      601 acgcttatcc ccgagcaata cactattggt tttttcactg gcgctcctgg gggcttcttt
      661 gtctttgcaa tttttattgc agtaacaaga ctaatacgag tcgcctatga gaaaaacaaa
      721 ggagaacaaa aatgaacgaa tacttaaaaa tatttttatc atccgcactt atagataacg
      781 ttgtttttat ccagtttcta gcgctctgtc cctttattgg tatgactgct gatgtgcata
      841 aatcgaacgg tatgggtgta gcaacttcct ttgtcatggt tttggcaacg gttgtaacct
      901 atcccctcta tcactttgtc ttactccccc ttgaccttgc gtttttacaa acccttgtat
      961 ttatcttggt tatcgcatcc ctcgtgcagc ttgtagagta ttacctaaaa aaatcagctc
     1021 cagcgctcta tagttcaatg ggagtctatc ttgccttgat aacaacaaac tgtgcaatcc
     1081 tcgcggttac cttaaaaagc attagcaagg gatacaactt cttagaatcc attgtatatt
     1141 ctcttggaac agcagtgggc tttttactcg caatggtgct tatggctggt atacgacaaa
     1201 aagtaaaact tgcaaaaata cctgcattta taaagggaac acctgtgctc ttcgtagcgg
     1261 caggcttgct ttcaatggct tttagcggct tttcaggcct cataaaataa ggaaaaaaag
     1321 atgataactg tcttatatac attagctgta tctgcaacac tcgccttgct acttggcttt
     1381 ttattgggct ttttcaaaaa aatctttttt gtagaagtag accccaaaat catgcttatc
     1441 agagacagcc tacccggtgc aaactgtggt ggttgtggct atcctggttg tgacgccttt
     1501 gccgaagccg ttgccgcagg tgaagcgaac ccatctggct gtaccgctgg gggtaaagat
     1561 accgcagata aggtttgcgc aataatgggt gtaagcgctg atgcccttga aacagtcgct
     1621 gtccttgcct gccaaggaag taaggaccac gctgttgcaa aaggcaatta cacaggagta
     1681 agcacctgtc gttctgccct catcggcaca aatggcataa aagtttgtga atgggcttgc
     1741 caaggttttg gcgactgtgt tacggtttgc ctcttcgatg caattcacat gggagacgat
     1801 ggtcttcccc atgtcgatga gacaaagtgt acaggttgcg gtatgtgtat catagaatgc
     1861 ccacaaaaac tcttagtaca cttcaataaa acagataaag gtgcctttgc tcgctgtaat
     1921 aaccgaaaca aaatgatacc acaaatccta aaaagctgta gcgtcggttg ttttaagtgt
     1981 ggtaaatgtg tacgtgcttg taaatacgat gcaattcacc tcgttgatgg tattccagtt
     2041 gttgactatg aaaaatgcac aagctgtaat gactgtgtcg aagcttgtcc tacaaatgtc
     2101 ctgtatttac gataaattta aacaatacaa taaataaaaa aagcccttaa gacagagtct
     2161 ttggggcttt tttcatataa accttgacaa tagcgccttt ttccatcatg attaaggtat
     2221 ggctcaacct gacaactccg gtaatttttt tcaaagactc atttctgcta tactgggctc
     2281 ttttgaccct gaggcagaaa aaaagcgcat tttgcgaagt atagcaaaag aagtaaacaa
     2341 aagtaagttt aagttctata aacactcctc tggagatgcc caagtgggac ttgctaagtt
     2401 tttttatgaa atatacaaaa acattggtgc ggcacaggta atgtttgaaa gcacacaaaa
     2461 cccaaatgcc ttcaaacatg cggttattga ctttgttatg aatgaaaaac aaagagaact
     2521 catcgagttt ttaaatgaac aagctatcct tgcacttgca caaacaatgc ctccaaatga
     2581 acttaagaaa aaaatacaaa ctgaccttga gactctttct caagaattcg atatccaaaa
     2641 aatacaaaaa attgatgctc tgtataccaa gcttatgctt tttcaatctt tttgcacctt
     2701 tgatttttat tttttactca aaaaatttga ttcaagtctt atggaacgcg actttaacta
     2761 tatgccgcgc tttgaaccaa tccgaggtga atatataacc gatgacctaa aagattttat
     2821 tgctgtagct tgggctatgc ccttaaacga agactggtca gaaatgcttg ctcttctaag
     2881 aaatcttaaa ggaattgaac caattgcagc caacacttgg aataaaacaa tacaaagact
     2941 tcgccaagtt caaaactcac aaattttaga aaaaattata aaacttacaa cctcagatcc
     3001 aagctatcaa gcctttatac cttcacgcca agaacgctta tttgagtcat atctagatgg
     3061 tcttaaaaaa gaagtacaag agaccctcgt aaaaattgaa aaagaagtta cgaattcccg
     3121 tgccgataat ttacttaaaa atatttttgg aacaactgcg gttatacgac tcaaatacta
     3181 cagtgaaaca gcaaacctta acctagaaaa aaaacgagtt cccctcttta agtatcatca
     3241 accgctcaat tacacaaaag cttttttgct tgactatttt aaaaaaaata ttcgcgaatt
     3301 ctccgacctt gttctcgttc gtggaaaatg ggtaacagct gtactatctg gacaaatgtc
     3361 agaagtgtat cagaacatcg ctgaaatttc cgatcaaatc acagaatttg acgaagcact
     3421 ttccgatgaa gcagcagtcg ggcaaaaatt aaaaaacctc ctcttacgag taggaagaga
     3481 tcgtgaagca gaacgaatta ttgcatccct tataaaagac acaaacgata aagctttatt
     3541 ttatataacg agcacggctc aaaaccttat cgcccttgca aaaacaataa aatctctttt
     3601 agaagataga gaaaagcctg aaccacaaat gcttatcaac tggaaagagc tcgataagtt
     3661 tgtcgatcat cctatacgtg acacgggtgt tgaaatctac aagatgatac accagttcat
     3721 acaacttata caaatctata tacccaaaaa ataaaaaaaa aggctaccgc atttggtagc
     3781 cctttttgtt ttaaagaaat ctttttgaat taaaacacta caacaacttc accgtttgga
     3841 tagcgctctg aaacccagtc agctattttt ttacttcgta gtgcttctac aagcgcctta
     3901 atatttgcct tagattcact tcctgccttt acggtaataa tgtttacgta gggagatttt
     3961 gctccttcga caagtaaacc atctttactt gcaactaaac ctgcaggaat tgcataattc
     4021 ccattgataa cagcagcatc gacatcagct aaaacacgtg ggagtgatgc agcttcaagt
     4081 tctcgaaata caaaattctt tggatttgcg acaatatcga gtggtgttgc acttaaatcg
     4141 ctcgaatctt ttaagctaat aagaccagca gaggccaaaa gcaagagagc tcttccttca
     4201 ttggttgggt catttggtat cgcaataact gccttttcag gtaaatctgc aagacttgca
     4261 actttttttg aataaagagc aattggctca atgtgaattt caccagcgtt tacaagatga
     4321 tagccttttt cttcattaaa agaatccata tacggaatat gttgaaaaaa gttagcatca
     4381 atttgcccac tttcaagggc ttcatttggt gtcacataat cggtgaattc aattaccttt
     4441 aggtttatac cttgtgcttt gaggtcggct gcaaccagat ttagcatttc agcatgtggc
     4501 tctggagtag ccccaacttt caataccgag tcatcctttt tcccggccgc aaagactccc
     4561 ccaaaaacaa gagagagcac aaggacaatc aaaataatcc ttttcataat ttctcctttt
     4621 aactcgctat ggatggcgag ttgcaattaa tctttgacat gttttgcaag gcaaaactgt
     4681 ccga
//
LOCUS       DAOSSW010000105         4670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_16681_length_4670_cov_5.445287, whole genome shotgun sequence.
ACCESSION   DAOSSW010000105 DAOSSW010000000
VERSION     DAOSSW010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16681_length_4670_cov_5.445287"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..>430
                     /locus_tag="PKW26_07625"
     CDS             3..>430
                     /locus_tag="PKW26_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOQ93473.1"
                     /translation="MSQIKINAQTILAPEILEKIQASVFEVIVEKPSEGNLEYEKSLP
                     FSRMAFAIRNDKYLPLGTAFLMNDGSFFSASHVFKLYEKTVYTNYYIRDRNGKIYKID
                     SITRLSTNRDYISFTVEAFTAENKAGLEYTKDIALNSTVFS"
     assembly_gap    431..440
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <441..1987
                     /locus_tag="PKW26_07630"
     CDS             <441..1987
                     /locus_tag="PKW26_07630"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HOQ93474.1"
                     /translation="VGNALGEGIVIRNGVLTSQTYEIVNGEWKWLRFSAAASPGNSGG
                     PLINSDGEVIGIITMKSENENLNYALPFSEVYALPASEAYLFQAIYYSLPNILSEKFY
                     HKISTTISLPAKLTEVQKKLTDYLNNYYKEIVLSLRKQFSPTGKKGFAFSSGWEEMLN
                     HDYLPNFPYVIYLPENNTWTYASPSNISDYKLENNGSVSYGSMMGYRMGLIKKPDSIA
                     LEDLLLQPELYMKYLLAASPIYRNLAGENIAITSLGKPSKSETYTDYFERTWLVNLWD
                     LPFADCMLISYALPLPEGMFVMYCHNTTGIITGSITLDIAFLADFVYPPYKTNVKNIE
                     EFLALSEQLVGKKPPFLQEISLQRNNTAFHFYNGSFGLNLSFDIFPIEDETYFSLTNG
                     FTFNKNKLKIDNRSFALYSNSRLENYRYIFVGEKIKPADTSLKKSLDQWNQKLNRISP
                     YNRQAYSYENYTYLDQTIYPKEIEYDTRKNADSLYVFALELDGQDRTEDILDFAEKVE
                     KILIFD"
     gene            complement(1984..3318)
                     /gene="ffh"
                     /locus_tag="PKW26_07635"
     CDS             complement(1984..3318)
                     /gene="ffh"
                     /locus_tag="PKW26_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOQ93475.1"
                     /translation="MLEKITHTFSDIFKKISGKASISEKNIEEAVEEIKMALLEADVN
                     LRVIRRFVNSTIDEAKGEKVLRSVNPGEQFIKIVHDKIVSLLGDSKQDLQLKGPDTQS
                     IILFLGLQGSGKTTSAAKLALRLKNEGRKPLLVACDLVRPAAIEQLSVLAEKISVPIY
                     KEETKDALKVAKNAITYAKKNALNTIIVDTAGRMQVDEGLMDEIKALKKLLNPDETIL
                     VADSMTGQTAVDVAKAFDEALSLSGVILTKFDSDARGGAALSLKSITGKPILFIGTGE
                     KVEDFEPFHPERIASRILGMGDIVSLVEKAQETIDEEEALALQKKMASNEFTLQDMLD
                     QLQRVKKMGSMQSLLQMMPGIAGQISEDDIDTSGMKYQEAIILSMTKKERDNHLIIGP
                     SRRKRIAKGSGTSVAEVNRLIKQFEKTRLTMKKVSKNKGMQAKMMSGLGGMF"
     gene            complement(3322..4362)
                     /locus_tag="PKW26_07640"
     CDS             complement(3322..4362)
                     /locus_tag="PKW26_07640"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOQ93476.1"
                     /translation="MKQIGILGAGAWGTAIAQVLAKNGNKVVLWAMEKEVVETINTRH
                     ENSLFLKGIQLHENITASQSIEEVAQDKEYLILASPSLYLASTVKKIAHLESLQNGTT
                     CIGVLTKGFIPSDTGPKLIVETLESVLPAVYQKSVVYISGPSHAEEVATGKLTGLIAA
                     SESPKNAIRFRELLKSESLMVFSSLDVIGVQICAAAKNAVAVVFGCLDAVTESSDFFG
                     DNTESLLLAAGLNEIQTLGFAMGATHAETFTSIAGVGDLDVTCRSPYGRNRKFGREII
                     LNNSLKDFKNLNDIIDNIDKIGYLPEGLVACKYIQEISEAKNLRLPICNGLYKILNKE
                     IEPIDFLKEVLF"
     gene            complement(4387..>4670)
                     /locus_tag="PKW26_07645"
     CDS             complement(4387..>4670)
                     /locus_tag="PKW26_07645"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOQ93477.1"
                     /translation="KPFYQNLLQYITSGPVIAMVWQADECVTLVRKLVGSTLVSEAAP
                     GTIRGDYCCHTPHNIIHASDTKENAEREVALFFNEEEICVWKDDAGCWY"
BASE COUNT         1475 a          927 c          751 g         1507 t
ORIGIN      
        1 gcatatctca aatcaaaatc aatgcccaaa caatacttgc accagaaata ctcgagaaga
       61 tacaagcttc tgtttttgaa gttattgttg aaaaaccttc tgaaggaaat cttgaatacg
      121 aaaagtcgct ccccttttct cgtatggctt ttgcaattcg caatgataaa tatttgccac
      181 taggaactgc ctttttaatg aacgatggaa gttttttttc tgcttcacat gtatttaaac
      241 tatatgaaaa aactgtttat acaaattatt atattagaga tcgaaatgga aaaatttaca
      301 aaatagattc tattacaaga ctctcaacaa atcgagatta tatttctttt acagtcgaag
      361 catttaccgc agaaaataaa gctgggcttg aatacacaaa agatatagca ctaaactcta
      421 cagttttttc nnnnnnnnnn cagtagggaa tgcacttgga gaaggcatcg ttattagaaa
      481 tggagtgctt acaagtcaaa cttatgaaat agtaaatgga gaatggaaat ggctgcgttt
      541 ttcagctgca gctagtcctg gaaattctgg tggtccactc atcaattcag acggagaagt
      601 aatcggcata attacaatga aaagcgaaaa cgaaaatcta aattacgctt taccattttc
      661 agaagtctac gcacttccag cttcagaagc ttatttattc caagcaatat attattcatt
      721 accaaatatt ttatccgaaa aattttatca taagatttca actacaatta gcttacctgc
      781 aaaactcaca gaagtacaaa aaaaactcac cgattattta aacaattact ataaagaaat
      841 tgttttaagc ttacgaaaac aattttctcc tacggggaaa aaaggctttg cattttcgtc
      901 aggctgggaa gaaatgctta accacgacta tctccccaat tttccttatg ttatttattt
      961 acccgaaaac aatacatgga cctatgcctc tccttcaaat atttccgatt ataaactcga
     1021 aaacaatggc tctgtttctt atggtagtat gatgggctat cgaatgggtt taataaaaaa
     1081 acctgattct atcgctcttg aagatttact tttgcaacca gaactttata tgaaatattt
     1141 gcttgctgca agtcctattt accgaaacct tgctggggaa aatattgcaa taacttctct
     1201 tggaaaaccc agtaaatcag aaacttatac cgactacttt gaaagaacct ggcttgtaaa
     1261 tctttgggac ttgccttttg cagattgtat gcttatctca tacgctttgc cgcttccaga
     1321 aggcatgttt gtaatgtact gccataatac tactggaatt attacaggaa gcataacatt
     1381 ggatattgca tttctcgcag attttgttta tccaccttac aagactaatg taaaaaatat
     1441 tgaagaattt ttagctttgt cagaacaact tgttgggaaa aaaccacctt ttttacaaga
     1501 gatttcgctt caaagaaata atactgcatt tcatttttat aatgggagct ttgggttaaa
     1561 tctttcgttc gatatttttc ctattgaaga tgaaacttat ttttcattaa ctaatggttt
     1621 tacgtttaat aaaaataaac tcaaaatcga taaccgatcc tttgctctgt atagcaattc
     1681 gcgattggaa aattatcgct atatctttgt tggagaaaaa attaaaccag ctgatacttc
     1741 cttaaaaaaa agtttagatc aatggaacca aaaactaaat agaatttctc cgtacaatag
     1801 acaagcatat agctatgaaa actataccta cctcgatcaa acaatttatc ctaaagaaat
     1861 agaatatgac acaagaaaaa acgcagacag tctttatgtc tttgctttag aattggatgg
     1921 acaagatcga acagaagata tattagattt tgcagagaaa gtagaaaaga tattgatttt
     1981 cgactaaaac attccgccta agccgctcat catctttgct tgcatgcctt tatttttaga
     2041 aacttttttc attgtgagcc gtgttttttc aaattgttta ataaggcgat ttacttcagc
     2101 aacagacgtg ccagaacctt ttgcaattct ttttcgcctg ctcggcccaa tgatgagatg
     2161 attatcccgc tctttttttg tcatcgaaag aataatcgct tcttggtatt tcataccgct
     2221 tgtatcaata tcgtcttcac ttatttgccc tgcaatccct ggcatcattt gtaaaagcga
     2281 ttgcatagag cccatttttt ttactcgttg taattggtca agcatatctt ggagcgtaaa
     2341 ctcattgctt gccattttct tttgaagtgc aagcgcttct tcttcatcga ttgtctcttg
     2401 cgctttttca acaagcgaaa caatatcgcc cattcccaaa atacggcttg caattcgctc
     2461 tggatgaaaa ggctcaaagt cttcaacctt ctctcctgta ccaataaata aaatgggttt
     2521 tccagtaata gattttaatg aaagcgcagc tccaccacgt gcatcggaat caaactttgt
     2581 gaggataact ccagaaagag ataatgcttc atcaaaagct tttgcaacat cgactgcagt
     2641 ttgacctgtc attgaatctg caaccaaaat tgtttcatca ggattcaaaa gtttttttaa
     2701 tgccttaatt tcatccatca agccttcgtc aacttgcatt cgccctgcag tatcgacaat
     2761 gattgtatta agagcatttt tctttgcata agtgattgca ttttttgcaa cctttaaagc
     2821 atcttttgtt tcttctttat aaataggaac agaaattttt tctgccaaaa ctgacaattg
     2881 ttcaatagca gcaggccgca ctaagtcgca tgcaacaagc aagggttttc gaccttcatt
     2941 ttttaaacga agtgcaagct ttgcagcaga agtcgttttc ccagaacctt gcaatcccaa
     3001 aaataaaata atagattgag tgtcaggtcc tttgagttgc aagtcttgtt ttgaatctcc
     3061 caaaagagaa acaattttat catgtacaat ttttataaat tgctcgccag gatttactga
     3121 acgtaaaact ttttcgccct ttgcttcatc aatcgtgcta tttacaaagc ggcgaataac
     3181 acgcaagtta acatcagctt caagaagggc cattttgatt tcttcaaccg cttcctctat
     3241 atttttttca cttatgctgg ctttgccaga gattttttta aaaatgtcac taaaggtatg
     3301 tgtaattttt tctagcatgg cttaaaacaa aacctccttt aaaaaatcta ttggctctat
     3361 ttccttattt aagattttat ataagccatt gcaaatagga aggcgtaaat ttttagcttc
     3421 cgaaatttct tgaatatatt tacaagcgac aagtccttca ggaaggtagc caattttatc
     3481 tatgttatcg attatatcat tgagattttt aaaatctttt aaactattgt ttagaataat
     3541 ttcgcgccca aactttcgat ttcgcccata gggacttcga caggttacgt cgagatcacc
     3601 aactcccgca atggaagtaa aggtttcggc atgtgttgcg cccatcgcaa aaccaagcgt
     3661 ttgaatttca tttaatcctg cagcaagtaa aagagattct gtattgtctc caaaaaaatc
     3721 tgaactttca gtaaccgcat caaggcaacc aaaaacaacc gcaacggcat tttttgcagc
     3781 agcacaaatt tgtacaccta ttacgtcaag gctagaaaaa accatgagac tttcagattt
     3841 aagcaattcc ctaaaccgta ttgcattttt ggggctttcg cttgcagcaa tcaaaccagt
     3901 tagttttccc gttgcaacct cttcagcatg actgggacca gaaatataca caacactttt
     3961 ttgatacaca gcaggcaaga cagactcaag tgtttcaacg atgagtttag gaccagtatc
     4021 tgaggggata aagccctttg tcaaaacacc tatacaagtc gtcccatttt gcaaactttc
     4081 taaatgcgct atttttttta ccgtacttgc caagtataaa gaaggacttg caaggatgag
     4141 atattcttta tcctgcgcaa cctcttcgat agactgactc gcagtaatat tttcatgaag
     4201 ctgaattcct tttagaaaaa gggagttttc atgccgcgta tttattgttt caacgacttc
     4261 tttttccatt gcccaaagga caaccttatt tccatttttt gccaaaacct gcgctatagc
     4321 tgttccccat gcgccagcac cgagtatacc gatttgtttc attttatatc cttacatacg
     4381 atgattttaa taccaacagc ctgcatcatc tttccaaaca cagatctctt cttcattgaa
     4441 aaaaagggcg acttctcgtt ctgcgttttc ttttgtgtca gaagcatgaa ttatattgtg
     4501 cggagtatga cagcaataat ctccacgtat agtaccaggt gctgcctctg aaacaagggt
     4561 actaccgaca agttttctca ccaaagtaac acattcatca gcttgccaaa ccattgcaat
     4621 cacaggacca gaagtaatat attgtaaaag attttgataa aacggctttc
//
LOCUS       DAOSSW010000106         4570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_17169_length_4570_cov_6.902547, whole genome shotgun sequence.
ACCESSION   DAOSSW010000106 DAOSSW010000000
VERSION     DAOSSW010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17169_length_4570_cov_6.902547"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PKW26_07650"
     CDS             complement(<1..401)
                     /locus_tag="PKW26_07650"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOQ93478.1"
                     /translation="MCLATKNCSPREFLVACCLVFFSYLLVAETSIRFIHMGAREGLV
                     NLGVSALVQDKTGFIWVGTQGGLQRWDGKSFTLYENEPFNTKTIPHNQIQTLFLDTDG
                     ETLWIGTYGGLAKLDTKTGFISSWSHEPAKP"
     gene            complement(615..2714)
                     /locus_tag="PKW26_07655"
     CDS             complement(615..2714)
                     /locus_tag="PKW26_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ93479.1"
                     /translation="MAEFFEADESVKELDITIIKRIFSYVRPYKILLILATLALVITT
                     VGELFIPILVQHTVDDALVVSYSALDRSAKEKLDPALPWDDAIELGDIFYVKKSFVSK
                     IDNQMRKSLIEEKLLETGPFYLTEWNTELSSLPKNIQEALLLRDDVFLKEGYFVASLD
                     YVQSLSAEDAKILRQEDQRTVNKNVAIFFALLIFVLLATFSQVWSSNLIGQRVMKALR
                     MDLFEKTLSQSLHFLSLQPVGRLVTRLTSDVDTINAFFSDVVIPFIKDISIMIGVLVT
                     LFILSPRLALICSLSLPPVLVVTSISRKKARDAFRKQRTWLSKVNAFISEHISGVSIV
                     KLFGRESSVSKEFASHNEELLKANLGEMYVYATFRPVIEFFSSTTMAILLYFGAGFHD
                     SGIVSLGTLIAFVNLLRMFYSPLQDIAEKYTMLQNAMAGGERVFKLLDTDERILDAPL
                     QSLVEPVKGRIEFDKVWFAYKGEDWVIKDLSFTVEAGESLAIVGYTGSGKTTLALLLS
                     RMWDIQKGEIRLDGVPIKNIPLRDLRRAIRPVMQDVFLFSGSIEENIGLGLDLTKIEI
                     EAALETVNAEIFVKQLPEREQTILGEGALTLSSGQRQLLSFARVVAHKPAIVVLDEAT
                     SSIDTETEQLVQKGLEGLLKGRTSIAIAHRLSTIKKADKIIVLSKGRLVEEGSHEELV
                     KKQGLYYALYKLQYEET"
     gene            complement(2715..4523)
                     /locus_tag="PKW26_07660"
     CDS             complement(2715..4523)
                     /locus_tag="PKW26_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ93480.1"
                     /translation="MWKEFKTLGPYFREHWKKYFFGLFFLVLVDAAQLLIPQFIKKSI
                     DSIAMGTGRSQSIVLPILLSLIGVALFISIGRYLWRFFIHGASRRIEADLRRRFFQKL
                     MYLPTSFYSKNTIGDLMARATNDMNAIRMASGMAAVAFIDGVFMSTAILIVLLTQNPQ
                     VSLYTIIPLPFVTILIIFFGRLVGKRFKKIQEDYSNMSTVVQESLQGVRVVKSFVKEP
                     YFASKFEKTNDDYKESSMKLVMTFGFFFPFVTFLGGLSTLILLLAGGTSLIQNQMSAG
                     DLTAMLTYLEMLLWPMLGAGFTVNMMQRGATSLKRVNEILNLEEEAEIAPGNELFDGV
                     LRAGDIEFRNLTLSFEAANKDALSNVSLQLAEGKCLGILGKIGSGKSVLIKSLSRLIE
                     PPKGSVFLGGVDVGSWPLSKLRKQMAFVPQDSFLFSASIKDNILFAQKDMENAEPTLE
                     EMDELLRISALDKDLALFPQGIETVVGERGLTLSGGQKQRVAIARALASEPEILVMDD
                     ALSAVDTETEEEILKALMQKRKGKTTILISNRVSTLKRANLVAVFDGGRLTQFGSPSE
                     LAKEKGFYAEVARMQALSESSEKEGREEQAITKERL"
BASE COUNT         1441 a         1016 c          810 g         1303 t
ORIGIN      
        1 tctggttttg ccggctcatg tgaccaactc gatataaaac ctgttttcgt atctaatttt
       61 gccaaaccac cataagttcc tatccacagg gtttcgccat ctgtatctaa aaaaagcgtt
      121 tgtatttgat tatgaggaat cgtttttgta ttgaagggtt cattctcata gagtgtaaaa
      181 ctttttccat cccatcgttg caacccgcct tgtgtcccaa cccatataaa gccggtttta
      241 tcttgtacaa gagcagaaac acccaaattc acaagcccct cacgtgctcc catatgtata
      301 aagcgaatag aagtttcagc gacaagtaaa taagaaaaaa aaactaaaca gcaagcaact
      361 aaaaactctc tcgggctaca atttttggtt gcgagacaca taccaagcac actccacgta
      421 gataattttt aactttttat tatattataa attagagtat accacaggtt taaaaaaaac
      481 aacagaaaag tcgtattttt ttgcggaaaa aggcatcctt ccctattaca tagttacata
      541 tgtatttatt tccttattga ggacttcaaa gaggatttcg ttcctttgaa ttgaaaacca
      601 tcctgatgaa aaaatcatgt ctcttcatac tgcaatttat acagcgcgta atacaagcct
      661 tgttttttta ccaactcctc gtggcttcct tcttctacaa gccgaccctt tgaaagtaca
      721 attatcttat cggctttttt aatcgtagaa agtcggtggg caatcgcaat gcttgtcctc
      781 ccttttagca atccttccaa gcctttttgc acaagctgtt ctgtttccgt atcgatagaa
      841 cttgttgcct catcaagcac cacaattgct ggcttgtgtg caacaacacg tgcaaaagaa
      901 agcagttgtc tttgcccaga agaaagcgtg agagctcctt ctccaagaat tgtctgctcg
      961 cgctccggaa gttgttttac aaaaatttct gcattgactg tctctaaggc agcctctatt
     1021 tcaatctttg ttaaatctaa tcccagtcct atattttctt cgatgcttcc agaaaacaaa
     1081 aaaacgtctt gcataaccgg gcgaatcgcg cggcgcaaat ctcttaaagg aatatttttt
     1141 atgggaactc catcaaggcg aatttcacct ttttgtatat cccacatacg acttaacaaa
     1201 agggcgaggg ttgtttttcc agacccagtg tagccaacaa ttgcaagact ttcgccagcc
     1261 tccacagtaa aggacaaatc ttttataacc caatcttcac ccttgtaggc aaaccatacc
     1321 ttatcaaatt cgatgcgccc ttttacaggc tcaactaagc tttgcaatgg agcatctaaa
     1381 atacgctcgt cagtatcgag gagtttaaaa acacgctcgc cgcctgccat tgcattttgc
     1441 aacatagtgt atttttctgc aatatcttgt aagggggaat aaaacatgcg aagcaagttt
     1501 acaaaggcaa tgagtgttcc aagcgaaaca atgcccgagt cgtgaaaacc tgcaccaaaa
     1561 tataagagaa tcgccatcgt tgtcgatgaa aaaaattcaa taacagggcg gaaggttgca
     1621 tagacataca tttcgccaag gtttgctttt agaagctcct cgttatgtga ggcaaattct
     1681 tttgaaaccg agctttctcg tccaaagagt tttacaattg aaacaccaga aatatgttct
     1741 gaaataaaag cgttgacctt tgaaagccat gtgcgttgtt ttcgaaaagc atcacgagct
     1801 ttttttcgtg aaatcgaagt taccactaaa acaggcggta agctcaagct gcatataaga
     1861 gcaagtcttg ggcttaaaat aaaaagcgtt acgagaacac ctatcataat agaaatatct
     1921 tttataaagg gaataacaac gtcagagaaa aaagcattta tcgtatcaac atcgctggta
     1981 agacgggtaa caaggcggcc cactggttgc aaggaaagaa aatgcaagga ttgcgacaga
     2041 gttttttcaa agaggtccat gcgaagcgcc ttcatcactc tttgcccaat gaggtttgaa
     2101 gaccacactt gggaaaaggt tgcaaggagg acaaaaatta aaagagcaaa aaagattgca
     2161 acatttttat tcaccgtcct ttgatcttct tgtcgcaaaa tcttagcatc ttcggcagaa
     2221 agcgattgta cataatccaa gcttgcaaca aaatagccct ctttcaaaaa aacatcatct
     2281 cgaagcagca gagcctcttg tatgtttttt ggaagactcg ataattctgt attccattcg
     2341 gtaagataaa aaggacctgt ctccaaaagt ttttcttcta tcaagctttt tctcatttga
     2401 ttatctattt tacttacaaa ggattttttt acatagaata tatctccaag ttctatggcg
     2461 tcatcccaag gaagagctgg gtctagtttt tcttttgcag acctgtcgag ggcactatag
     2521 gaaaccacaa gcgcatcatc gaccgtatgt tgcactaaga taggaataaa gagttcaccc
     2581 acagtggtaa taacaagggc aagtgttgca agaattaaaa gtattttata ggggcgcaca
     2641 taagaaaaaa ttctttttat aatagttata tctagttctt ttactgattc atcagcttca
     2701 aaaaactctg ccatttataa acgctcctta gtaattgcct gctcttctct cccttctttt
     2761 tcagaacttt cactcaaggc ttgcatacga gcaacctctg cataaaaacc tttttctttt
     2821 gcaagctctg acggcgagcc aaactgcgtg agccttccac cgtcaaaaac cgcaacaaga
     2881 tttgcccgct tcaaagtaga aaccctattt gaaataagta ttgtcgtctt gccttttcgc
     2941 ttttgcataa gcgccttcag aatttcttcc tctgtctctg tatctaccgc agaaagcgca
     3001 tcatccataa ccaaaatttc aggctcactt gccaaagctc gtgcaatagc tacccgttgt
     3061 ttttgcccac ctgaaagcgt cagtcctcgc tctcccacca ccgtctcaat gccttgtgga
     3121 aaaagtgcga ggtctttatc gagagcagag atacgcaaaa gctcatccat ttcttctaaa
     3181 gtcggttcag cattttccat atccttttgt gcaaataaaa tattatcctt tatacttgca
     3241 gaaaataaaa acgaatcctg cggcacaaag gccatttgct ttcgtagctt tgaaagcggc
     3301 caagaaccaa catcaactcc accaagaaag acagagccct ttggcggctc tatgagacga
     3361 gagagagatt ttattaacac agattttcca ctgcctattt ttccaagaat gccaagacat
     3421 ttcccctcag caagctgcaa ggaaacattt gaaagagcat ctttatttgc agcctcaaaa
     3481 gaaagcgtga gatttctaaa ctcaatatca cccgctctaa gaactccgtc aaaaagctcg
     3541 ttgcctgggg caatctcagc ttcttcttcc aggtttaaaa tttcgtttac gcgctttaaa
     3601 ctcgtcgctc ctcgttgcat catgtttacc gtaaaacctg ccccaagcat tggccacaaa
     3661 agcatttcca aatacgtgag catggctgtt aggtcgcccg cgctcatttg attttgtatg
     3721 agagaggttc cacctgcaag caataaaata agcgtcgaaa gccctccgag aaatgttaca
     3781 aatggaaaga aaaaaccaaa ggtcataaca agcttcatgg agctttcttt gtaatcatca
     3841 tttgtctttt caaacttaga agcaaagtag ggctctttca caaaagattt tacaacacgc
     3901 actccttgca aactttcttg cacaacagta ctcatattag aataatcttc ttgaattttt
     3961 ttaaaacgtt ttccaacaag gcgaccaaaa aatataatca agatagttac aaagggcagt
     4021 ggaataatcg tatataaact cacctgtgga ttttgtgtaa gcaagacaat aagaatcgcc
     4081 gtcgacataa acacaccgtc aataaaagca acagccgcca tgccacttgc cattcgaatc
     4141 gcattcatat cgtttgttgc acgagccatc aaatcgccta tcgtgttttt agaatagaag
     4201 gaagttggca aatacatcag tttttgaaaa aatcgcctac gcaaatctgc ctcaatgcgc
     4261 cgagaggcac catgaataaa aaagcgccac agataacgac ctatactaat aaataatgca
     4321 actccaatga ggctcaaaag aataggcaaa acaatacttt gactgcgccc cgttcccata
     4381 gcaatgctat caatcgattt tttgataaac tggggaataa gcaactgtgc tgcatcaaca
     4441 agaaccaaaa aaaagagtcc aaaaaaatat tttttccaat gttccctaaa ataggggccg
     4501 agtgttttaa attctttcca catagaatat ttagtgtata cctattatag agttttgata
     4561 agagggggaa
//
LOCUS       DAOSSW010000107         4495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_17515_length_4495_cov_8.572748, whole genome shotgun sequence.
ACCESSION   DAOSSW010000107 DAOSSW010000000
VERSION     DAOSSW010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17515_length_4495_cov_8.572748"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..3313)
                     /locus_tag="PKW26_07665"
     CDS             complement(152..3313)
                     /locus_tag="PKW26_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOQ93481.1"
                     /translation="MNISKTIVNKPTTVLIIFVVLSALGLYTLKDLPLDLLPDMELPF
                     VAIVTSYPGAGPEEVEKRVTRLVESAISGVSGIDQMGSTSMSGSSLVYIQFLFGINMD
                     EAMNDVRASLDFIKRALPEEVGSPMIMKMDPNLLPIMSYSISGNRSPEELRNYAEEIA
                     PKLEQIEGLAQASVGGGRESAIIVEIPRDRLQAYNLTISQVSQMIAAQNIQIAGGTIT
                     EEDINYTISTIGEYSSIEDIKNTVISYKMAGMTYTAMGAIPGSLTTVRLRDIADVYEG
                     YKKPSSLHYLNGVPSVSLTLQKQSGKNSVQTARAIKARMKNIIKEAPSDINITLTLDT
                     TSIIERSVSQVASSAMQGGLLAILILFIFLRSLRSTVIIGLTIPISLVITFGIMFFSG
                     MTLNIMTLAGLALGIGMLVDNSIVVLENIYSYRDKGAKPKVAAILGSQEMIMAISAST
                     LTTICVFLPLIMYQKELGIIGEVFKGLSFTVVISLLCSLVVAVVFVPVLSSSYLKIGS
                     TKEKNLKGIWLLLDTSFERFFTWLENKYFSAIQYVLAHKKKTLATIIGLLVLSIVIIP
                     FLGFEFMPESASDSVTVNVSLPVGTKLETTEAVLQQLEQIVYREIIGYENVTLSVGSG
                     GLLTFGAGSSDSGSMTINLPPFSKRIESEDEIKTILRKHFSDFPGVVLSIGDSSMMSL
                     GGGSSAISIDIKCENLDKARDTARTIQEVLKNEASHLVTEPSISLQDGLPQVNIILDR
                     ERMYNLGLNVYSVGQEIKANIAGSTASRFRQDGKETDIVIMLSESDKTQLDDLEHIFV
                     TNASGDRIPVSSFAKYEEGRSPISINRHDQSRMISVTMGLNKNLSLTKVQKEIEALVQ
                     KHIPSDPDLRIEYSGDYQALIKAIKQFIVIIAMAIILVFAVMASQFESLLDPFIVLFT
                     MPLVVIGIVAVYAITGLKLNVMTAVGVLILVGIIVNNGIVLVDYTNLLRKRGLPLIEA
                     CAEAARNRLRPILMTTLTTVLGLVPMAFFPGEGSEMVQPIGQTVLGGLSFGTLMTLFL
                     MPVLYAIFNQMREKKAQAKEVNA"
     gene            complement(3328..4260)
                     /locus_tag="PKW26_07670"
     CDS             complement(3328..4260)
                     /locus_tag="PKW26_07670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOQ93482.1"
                     /translation="MNKKKRIKKIKLSSTIVLSFSLLVFIACSKESASDEKIIEEKAV
                     FAVSAFTVEETRIENYLEFGGDIIAKTSLDINPEVQGKIVELKIAVGDYVEKDAVIAL
                     VDASRAGMSFALGQIKAPISGTITQVNVVVGSMVAPQLAVAKVSKLDTLLVSMNVPER
                     YVSKIKQEQKAELRFDAYPERFYSASVSEISPVLDATSRTMNVKLSLDRTGQNDGAVK
                     IGMFARVKLVTEVHENAVIIPDAAIVNRFGESFVFVIDENMSVEKRNIVRGIRVDEKS
                     EILSGLEAGEKLVVRGQTLLEEGASVHIVSEISF"
     gene            complement(4286..>4495)
                     /locus_tag="PKW26_07675"
     CDS             complement(4286..>4495)
                     /locus_tag="PKW26_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOQ93483.1"
                     /translation="AISVMEMNESIAKKALALAEDSYKAGLLEYLDLRDAETSLQQAQ
                     LGVLSEKYNYLTTMLDLEKAINTKW"
BASE COUNT         1406 a          931 c          757 g         1401 t
ORIGIN      
        1 tcaggccaaa ctgaatcgcc catcttcggt gtagaaaatc ctttgccatg taccgtgggt
       61 atgagggtaa aaaattgggc ttcggggata tctttgagtg agccaaagat atcttcttcg
      121 atagattgat taaagataat ttcaattcgt ttcatgcgtt tacctctttc gcttgtgctt
      181 ttttctctct catttgatta aaaatcgcat acaataccgg catgagaaat aatgtcataa
      241 gagtgccaaa agaaaggccg cccaaaacgg tttgcccgat cggttgaacc atttcactac
      301 cttcgccagg gaaaaatgcc ataggaacga gcccgagcac tgttgtcaaa gtagtcatca
      361 aaataggacg gaggcgattt cgagcagctt ctgcacaagc ttcgatgagg ggaagcccgc
      421 gttttctcaa aagatttgta tagtcaacta acacaatgcc attattgacg atgattccga
      481 caaggatgag cacgccaacg gctgtcataa cattgagttt taagcctgta attgcataga
      541 ctgcaacaat gccgataaca acaagcggca tcgtaaaaag cacaataaaa ggatcgagca
      601 aggattcaaa ctggctagcc ataactgcaa ataccaaaat tattgccatt gcgataataa
      661 ctataaattg ttttattgct ttgataagtg cttggtaatc gccactatat tctattcgaa
      721 gatcaggatc tgatggaata tgtttttgca caagtgcttc gatttctttt tgtacctttg
      781 taagagacag gtttttgttc aagcccattg taactgaaat cattcggctt tgatcatggc
      841 gattgatgct aataggagac ctgccttctt catattttgc aaaactagaa acaggaattc
      901 gatcgccact tgcattggtt acaaaaatat gttccaaatc atcaagctgt gttttatcac
      961 tttcagaaag cataatcaca atatctgttt ctttgccatc ttgacggaac cgactcgcag
     1021 tcgagcctgc aatattcgct tttatttcct ggccaacaga atagacattt aagccaagat
     1081 tatacattct ctctctatct aaaataatat taacctgggg caagccgtct tgtaaagaaa
     1141 tacttggctc agttaccaaa tggctcgctt catttttcaa aacttcttgt attgttcgtg
     1201 cagtatctcg agccttatca agattttcgc attttatatc aatagaaatt gcagaagagc
     1261 ctccgcccaa gctcatcatt gagctatctc caatgcttaa aacaacacca ggaaaatcag
     1321 aaaaatgttt tcgtaaaatt gtttttattt catcttcgct ttctattcgc tttgaaaaag
     1381 gcggaagatt tattgtcatt gatccagagt cagacgagcc tgcaccaaaa gtcagaagcc
     1441 caccagaacc aaccgatagc gttacgtttt catagccaat aatttctcga tagacaattt
     1501 gttcgagctg ttgtaaaaca gcttctgttg tttcaagctt tgtgccaact ggtaagctta
     1561 cattgactgt aacagaatcg cttgcagatt cgggcataaa ctcaaagcct aaaaatggaa
     1621 tgattacaat actcaaaaca agcaatccaa taattgtagc gagcgttttc tttttatgtg
     1681 caagcacata ttgaattgca gaaaaatatt tattttcaag ccaagtaaaa aatctttcaa
     1741 aacttgtatc tagcagaagc caaatacctt tgagattttt ttcttttgta gagccgattt
     1801 tcaaatacga acttgacaaa acaggaacaa aaacaacagc aacaacaagt gagcaaagaa
     1861 gtgaaataac aactgtaaaa gataaacctt taaatacttc accaataatg ccgagctctt
     1921 tttgatacat tatgagcggt aaaaaaacac aaatcgttgt taaagtcgat gcagaaatcg
     1981 ccataatcat ttcttgcgaa ccaagaattg cggcaacttt tggctttgca cccttgtctc
     2041 gatatgaata aatattttcc aaaacaacaa tcgagttatc gacaagcatt cctattccca
     2101 atgcgagccc cgcaagtgtc ataatattga gtgtcatgcc agaaaaaaac ataatgccaa
     2161 acgtaattac gagcgaaatt ggaattgtca aaccaatgat gacggtactt cgaaggcttc
     2221 ttaggaaaat aaataaaata agtattgcaa gaagtcctcc ttgcatagca ctcgatgcaa
     2281 cttgagaaac tgagcgctca ataatacttg tagtatcaag agtgagcgta atgtttatat
     2341 ctgatggagc ttctttaatt atgtttttca ttcgtgcttt tattgcacga gcagtttgta
     2401 cagaattttt cccactttgt ttttgcagag ttaatgaaac actgggaacg ccatttaaat
     2461 aatgtaaaga cgaaggtttt ttatagcctt catagacatc tgcaatatca cgaagtcgaa
     2521 ctgttgttaa gcttcctgga attgcgccca tcgcagtata agtcatgcca gccatcttat
     2581 acgaaataac cgtattttta atatcttcta tagaagaata ctcaccaata gtgctaattg
     2641 tataatttat atcttcttct gttattgttc cacctgcaat ttgaatattt tgtgcagcaa
     2701 tcatttgtga aacttgcgaa atcgtcaaat tatatgcttg cagtctatct cgaggaattt
     2761 ctacaataat ggcactctct ctgccacctc caacagatgc ttgtgcaaga ccttcgattt
     2821 gttcaagctt gggtgcaatt tcttctgcat aatttcgcaa ttcttcagga cttctatttc
     2881 cacttatcga ataactcata atgggtaaaa gatttggatc cattttcata atcataggac
     2941 ttcccacttc ttcaggaagt gctcgcttta taaaatccaa acttgcacgc acgtcattca
     3001 tcgcttcgtc catatttatt ccaaaaagga actggatata aaccaagctt gaaccgctca
     3061 ttgaagttga gcccatctga tcgataccag aaacaccaga aatcgcagat tcgacaaggc
     3121 gtgttacccg cttttcaact tcttcaggtc ctgctccagg atacgaagta acaatagcaa
     3181 caaaagggag ttccatgtca ggaagtaaat caagaggtaa atctttgagc gtatatagcc
     3241 caagagcaga taaaacaaca aagataatta atacagtcgt cggtttgttt acaatagttt
     3301 ttgaaatatt catctatact tctccattta aaacgaaatt tcagaaacta tatgaaccga
     3361 agcgccttct tcaagaagcg tttgccctcg aacaacaagt ttttctcccg cttctaagcc
     3421 tgaaagaatt tctgattttt catcaactcg tatgcctcgc acaatatttc ttttttctac
     3481 actcatgttt tcgtcaatta caaacacaaa gctttctccg aatcggttta caatagcagc
     3541 atcaggaata atgactgcgt tttcatgaac ttcggtaaca agttttactc gtgcaaacat
     3601 tccgattttt accgcaccat cattttgtcc agttctatct aatgaaagct ttacattcat
     3661 ggttcgagac gtagcatcaa ggacaggaga aatttctgaa acagaggctg aatagaaacg
     3721 ttcgggatag gcatcaaatc gaagttcagc tttttgctct tgttttattt ttgaaacata
     3781 acgctctggc acattcatcg aaaccaaaag cgtatcaagc ttactcacct ttgcaacagc
     3841 aagctgcgga gcgaccatgc ttccaacaac aacattgact tgtgtaatcg ttccagaaat
     3901 tggcgctttt atttgcccga gtgcgaaaga catacctgca cgacttgcat caactaatgc
     3961 gataactgca tctttttcaa catagtcacc tacagcaatt tttagctcaa ctatttttcc
     4021 ttgcacttca ggatttatat ccaagcttgt ttttgcaatt atgtcgccac caaattctaa
     4081 ataattttct attctcgttt cttcaacagt aaacgcactt actgcaaaca ctgctttttc
     4141 ttcaattatt ttttcatcag atgcgctttc ttttgaacac gcaataaaaa ctaataaaga
     4201 aaaactcaaa acaatagttg aactgagttt aatttttttt attctctttt ttttattcat
     4261 ttttcctcca aaacaaaaaa gctttttacc attttgtatt gattgctttt tctaaatcaa
     4321 gcatagttgt tagataatta tatttttcac taagcacgcc caattgcgct tgttgcaaac
     4381 ttgtttctgc atctcgtaaa tctaaatatt cgagcaagcc tgctttgtac gaatcttcag
     4441 ccaaagccaa agcttttttt gcgatagatt cattcatttc catcacagaa attgc
//
LOCUS       DAOSSW010000108         3933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_20753_length_3933_cov_6.510572, whole genome shotgun sequence.
ACCESSION   DAOSSW010000108 DAOSSW010000000
VERSION     DAOSSW010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20753_length_3933_cov_6.510572"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="PKW26_07680"
     CDS             <1..1123
                     /locus_tag="PKW26_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOQ93484.1"
                     /translation="AIRPQWMCGVPRLWEALAQGIYKNMQKEGGIKLALFNFFISVGK
                     KWVWSYEHISGRVCRFTKRVRLFDFLAGIIPFLLLSPLYGLGEVLIYKKIKARLGGRF
                     VCAISGGGALQPETDAFYRAIGFRLLEGYGITETAPILAFRSALHKARPGCVGYVYPS
                     AELKVVAESGGEILSMEALPPGKKGLVMAKGDSVMKGYYNSPELTAKAIDADGWFNTG
                     DLGMMTYDNELKITGRAKDTIVLLGGENIEPQGIEAAMGASQYIETSVLVGQDKKYLA
                     ALIVPVKDAVLAYANENGILNESYEALLESPEIINLIREEIDTRISKANDFRVCERVF
                     KFTLLPESFTVGRELSGKQELMRHKIVELYKEEVDSLFV"
     gene            1288..3327
                     /gene="fusA"
                     /locus_tag="PKW26_07685"
     CDS             1288..3327
                     /gene="fusA"
                     /locus_tag="PKW26_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOQ93485.1"
                     /translation="MLSHMRNIGIMAHIDAGKTTTTERILYYTGKIHRIGEIDDGAAT
                     MDWMSQEQERGITIQSAATTTYWKDCQINIIDTPGHVDFTAEVERSLRVLDGAVAVIC
                     AVGGVQPQTETVWRQANEYKVPRLCFVNKMDRVGADFYAALDDVAEKFGETCLALVLP
                     IGKESNYEGLIDLIRMKELRFDVESEGEIIHESEIDASRRAEAEEWREKIIDALSMHN
                     DEIAELYLSGEDVPEALIIKQIRQSTIERHFIPFFCGSARKNSGIQPLIDAIVAYLPA
                     PDEVAPAIGINVKKEESITIPCDADAMPLGLIFKIQHEREAGSLAYIRMYSGKIKSGE
                     QVYNIGKKKRERVNRLLRMHSNKSDPLDSVSAGDIAVIIGLKQAQTGDTIGSEGVPIL
                     LESMHFPEPVISVSIEPETLSDRDKLKETLDILSKEDPTFISRDDKETGQLIISGMGE
                     LHLDVLVTRMLEDFKLQAKVGKPQVTYRESISGEAQHTENYEKLLGGKEQTASVSLLV
                     KPATSEGNSYSNSLRMPEIPQEIFDAIKRGVENSFMSGIQYGYPCTNISVELVDLDYS
                     ELTSTAFAFEACTVQAFDAACKKASPEILEPIMNVDILSPKEFVGDAMSQITQRGGLI
                     LSLDSKPSIEVVHAQAPMEKMFGFSTSLRSVTQGRASFSMAFSHFQIKQGGLNFF"
     gene            3342..3932
                     /locus_tag="PKW26_07690"
     CDS             3342..3932
                     /locus_tag="PKW26_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ93486.1"
                     /translation="MIQERLHKQMSFIREIEKLKVVYRQNGTMGNKRQENSAEHSWHI
                     AMMALVLQEYFPHKVDVFRVVKLLLIHDIVEVYAGDTFLYDDKKRLDAKKIEKEAAKK
                     IFSLLPEEQAEDFYTSWLEFEEVTTKEAEFAKILDNLQPIINHYTTRNQNIIGKELTK
                     RQIAAKKSFIKELAPELWEYADMILDKSVEIGLYKA"
BASE COUNT         1243 a          679 c          896 g         1115 t
ORIGIN      
        1 agcaattcgt ccacagtgga tgtgtggtgt acctcgattg tgggaagctc ttgctcaggg
       61 tatttataaa aatatgcaaa aagaaggtgg tataaaactt gctcttttta acttttttat
      121 atctgttggg aaaaaatggg tatggtccta tgaacatatt agcggacggg tttgtcgttt
      181 tacaaaacga gttcgtctct ttgatttttt agccggtatt attccttttc tccttttaag
      241 cccattgtat ggattggggg aagtgctcat ttacaagaaa ataaaagcac gtttaggcgg
      301 acgctttgtc tgtgctatct ctggaggcgg tgcgctccaa cctgaaaccg atgcttttta
      361 tagggcgatt ggttttagac ttttggaagg ttatggtata acggaaaccg ctcccatcct
      421 tgcttttaga agtgccttgc ataaggcgcg ccctggttgc gttggctatg tctatccctc
      481 tgctgaatta aaggttgttg cagaaagcgg cggtgagatt ctttctatgg aagctctgcc
      541 tccagggaaa aaaggattgg ttatggcaaa gggcgactct gttatgaagg gctattataa
      601 tagcccagaa cttacggcaa aggccatcga tgcagacggt tggtttaaca ctggcgacct
      661 cggtatgatg acctacgaca atgagcttaa aataactggt cgagcaaaag acacgattgt
      721 cttactcggt ggagaaaata tagaaccgca aggtattgaa gctgctatgg gtgcaagcca
      781 atacatcgaa acttcggttc ttgttgggca agataaaaaa tatctcgctg ccttaattgt
      841 tcctgttaaa gatgctgttt tagcttacgc aaatgaaaat ggcatcttaa acgaaagcta
      901 tgaggctctt ttggaaagtc ccgaaattat caatttaatt cgagaagaaa tagacacaag
      961 gatttcaaaa gcgaacgact ttagagtttg cgagcgtgtg tttaaattta cgcttttgcc
     1021 agaatctttt acggttggac gagagctttc aggtaaacaa gaattgatgc gacataaaat
     1081 agtagaactt tataaggaag aagttgattc tctctttgtt tagaattctt ttataattta
     1141 gaaagggctg ccttaggaaa aatctggggc agcttttttt atatcctcgt tttatttttg
     1201 ttatgcttga gtatgcgcat gtttttaagt atactgaatg tattgattaa aacgatagtg
     1261 cagaatttta gcataggaag gattattatg cttagtcata tgagaaatat tggcatcatg
     1321 gcgcacattg atgctggaaa aacaacaact acagaacgca tcttgtatta tacgggaaaa
     1381 attcatcgta taggtgaaat agatgacggc gcggcaacga tggattggat gagccaggag
     1441 caagagcgtg ggattaccat acagagtgct gcaacgacaa cctactggaa ggattgtcaa
     1501 ataaatatta tagatacgcc agggcatgtg gattttaccg cagaagtaga gcgctctcta
     1561 cgcgttttag atggagctgt tgcggttatt tgtgcggttg gtggagtgca accacaaact
     1621 gaaacagtct ggcgtcaagc aaatgaatac aaggtacctc gtctttgctt tgtaaataaa
     1681 atggatagag ttggtgcgga tttttatgcc gctctggacg atgtggccga aaaatttggc
     1741 gaaacctgcc tcgccttagt tttacccatc ggaaaagaaa gcaattatga aggtcttata
     1801 gatttaatac gcatgaagga actgcgattt gatgtagaaa gcgagggtga aattatccat
     1861 gagtcagaaa tagatgcatc gagacgtgct gaggcagaag aatggcgcga gaaaataatt
     1921 gatgcgcttt ctatgcacaa tgatgaaata gctgaactct atctttcagg ggaagacgta
     1981 cccgaagcac ttattataaa gcaaatacgg caatcaacaa ttgagaggca ttttattccc
     2041 tttttttgtg gctcagcgcg aaaaaattct ggtatccagc cgcttatcga tgcgattgtt
     2101 gcatacctgc ctgctccaga tgaagttgct cctgctattg gaatcaatgt aaaaaaagaa
     2161 gagtccatta caattccctg cgatgcggat gcaatgcctt tggggcttat ttttaaaatt
     2221 caacatgagc gtgaagcagg aagccttgcc tatattcgta tgtatagtgg aaaaataaaa
     2281 agcggggaac aagtatacaa tatcggcaag aaaaaaagag aacgagtaaa tcgtctttta
     2341 cgcatgcact caaataaaag cgatccgctt gattcagtga gcgctggcga tattgcggtt
     2401 attattgggc ttaaacaagc tcagacggga gataccatcg gcagtgaggg agtacctatt
     2461 ttgcttgaat ccatgcattt tccagagccg gtgatttcgg tatcgattga gccagaaacc
     2521 ttatctgatc gcgataaact aaaagaaacg ctcgacattc tttctaaaga agaccctacc
     2581 tttatttcac gagatgataa ggaaacaggg cagttaatta tttctggtat gggagagctc
     2641 caccttgatg tgcttgttac acgaatgctt gaagatttta agctacaggc aaaggttggg
     2701 aaaccgcagg taacataccg agaatctatt tctggagaag cgcagcatac agaaaactat
     2761 gaaaaactgc ttgggggcaa agaacaaaca gcttcggtta gccttttggt taagcctgct
     2821 acaagcgagg gaaatagcta tagcaattcg cttcgaatgc ctgaaatccc tcaagaaata
     2881 tttgacgcaa ttaaacgtgg ggtggaaaat tcatttatga gcggtattca atacggctac
     2941 ccctgtacaa atatttcggt agagcttgtc gacttagact attcagagct cacctctaca
     3001 gcctttgcct ttgaagcttg tactgtgcaa gcctttgatg cagcttgtaa aaaagcaagt
     3061 cctgaaatcc tagagcctat tatgaatgtc gatatcctca gccctaaaga atttgtgggg
     3121 gatgcaatga gccaaattac acaacgaggt gggcttatat taagcttgga ttcaaaacca
     3181 agcatcgaag tagttcatgc acaagcaccc atggaaaaaa tgtttggctt ttcgacaagt
     3241 ttgcgctcag ttacgcaggg aagggcttct tttagtatgg cttttagcca ttttcaaata
     3301 aaacagggtg gcttaaactt tttttaataa atggaaggta tatgatacaa gaacgcttgc
     3361 ataaacaaat gagctttatt cgagaaattg aaaaacttaa agtggtatat agacaaaatg
     3421 gaacaatggg taacaaacga caagaaaatt cagctgaaca ttcttggcat attgcaatga
     3481 tggcccttgt tttgcaagag tattttccgc ataaggtgga tgtttttcga gttgtgaaat
     3541 tacttttaat tcacgacatt gtcgaagtat atgcaggaga tactttttta tatgacgata
     3601 aaaaacgtct tgatgcaaaa aaaatagaaa aagaagccgc aaaaaaaata ttttcactct
     3661 tacctgaaga gcaagcagaa gatttttata caagttggct tgagtttgaa gaagttacaa
     3721 caaaagaagc tgagtttgca aaaatactcg acaatttaca gcctattatc aatcactata
     3781 caacacgaaa ccaaaatatc ataggaaaag aactaacaaa aaggcaaata gctgcaaaaa
     3841 aatcttttat aaaagaactg gctcccgaac tttgggaata tgcagatatg attttggata
     3901 aaagcgtaga aatcggttta tataaagctt aaa
//
LOCUS       DAOSSW010000109         3930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_20780_length_3930_cov_5.590710, whole genome shotgun sequence.
ACCESSION   DAOSSW010000109 DAOSSW010000000
VERSION     DAOSSW010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20780_length_3930_cov_5.590710"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PKW26_07695"
     CDS             <1..558
                     /locus_tag="PKW26_07695"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOQ93487.1"
                     /translation="EVAQETGEGAERNFIIPNDSDNPDSFNWKDGDEVEFFFRYDDGE
                     SGSFTFDHDGNPETDPVPLYFFRLQDPKDPSSVDLWRFSLSDIRRQRGGITILNNVIN
                     SLKKEETIVELDIKKAGNVTVHVLTLDGNIVKTLQRGRLNPGLHYFKWNGTNLSGTPV
                     ARGIYFMRVVGPDIDETRKVMVIKE"
     gene            608..988
                     /gene="queD"
                     /locus_tag="PKW26_07700"
     CDS             608..988
                     /gene="queD"
                     /locus_tag="PKW26_07700"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOQ93488.1"
                     /translation="MFEVRVEADFAAAHFLQDFHGKCERLHGHNYQVFAHARGTDLDE
                     GGMLLDFGILKKELRSICKELDHSHLNENAFFENNPSAERIALYIFQKLESALQEYSP
                     KTAALLYAVDVFETATSRARYTKD"
     gene            complement(993..1760)
                     /gene="hisF"
                     /locus_tag="PKW26_07705"
     CDS             complement(993..1760)
                     /gene="hisF"
                     /locus_tag="PKW26_07705"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOQ93489.1"
                     /translation="MKNDPKKIIICLDIKDGKVSKGVQFENVISLGDPVEMAENYSKQ
                     AVDELVFYDIMASALGKGPNFPLIQRVREKTSVPLCVGGGVASIEDIKKLKDCGVDKI
                     SINSPAVRNPDLLAEAAKNFGSESLVLGIDAKKDASCKNGYRVMLGGGKTPTDLDVLE
                     WAEKAVALGAGEIVLNSIDADGTKAGYDIELTKLIAEKVSVPVVASGGGGSIEHIAEV
                     LSKGSASAALIASIAHLKIHTVGEIKAELKKRGIQVL"
     gene            1827..2048
                     /locus_tag="PKW26_07710"
     CDS             1827..2048
                     /locus_tag="PKW26_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93490.1"
                     /translation="MDTENLFALVLNVIKNKYIIFIFIVVFLYLSFFLYVVNYRKKPR
                     KIKVKKLKEPAPPKPSSEEEEDEVDDEAT"
     gene            complement(2041..3687)
                     /locus_tag="PKW26_07715"
     CDS             complement(2041..3687)
                     /locus_tag="PKW26_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93491.1"
                     /translation="MKRISIVFSVFLLILISLSSCASSKIRRANPIQRAYSAAQLLLK
                     GSTGDAYIKQFNIEMDKAKADINEKITRVNLNPEAYEVVADSIDDWLQLYELIAILES
                     RYPGGLYGKKQSAFFSYIDYKPLKETANKYTAEKYFTQARSIAERPHSLNETISAVEY
                     LHKSLSYSNHLLEETQTLGASLSYEAALKLYETKTSDSLKNAHAYFLEADSWIAHYAD
                     ARDRANNMLPEIATILISEADSLLSIEDYSRLRKAYLIYEEAFAYYPSLAEEKLNETK
                     EKLTITVALIFSGANPDFIHDDEIREALENNFANTTEGPKFVHIDFIYTPYATTNHAS
                     FLSSIISTLGSQSTNLKKNYSSYDLVLIPGPKFNTAIETIENPVKEIEKLETYYRQTT
                     ISEDGDKQIFVSEVSKNEYTNPKSNYTDGDKNYQFIYWKEEGTITRYVQKVSLFMDYS
                     YELYDMRNNFVNYMLSFDRSGDRLDLEFVQESYVGPANLQPEKLRNDAFYMPGQNIWY
                     AYKIPTLINPYQIIDTSYNQINENGRKLFRQIRLLSYKAK"
     gene            complement(3716..>3930)
                     /locus_tag="PKW26_07720"
     CDS             complement(3716..>3930)
                     /locus_tag="PKW26_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93492.1"
                     /translation="CYDMEEATDTIVKTCNEKAAQKYKMRIWESADIREIIQNLLFEK
                     KISISPNPWKGKISIAKRKKNKIKTR"
BASE COUNT         1112 a          767 c          742 g         1309 t
ORIGIN      
        1 gaagtagcac aagaaactgg ggagggtgct gaacgaaact ttatcattcc aaatgattct
       61 gataatccag attcgttcaa ttggaaggat ggagatgagg ttgagttttt cttccgctac
      121 gatgatggag agtctggcag ttttaccttt gaccacgatg gaaaccctga aactgatcca
      181 gtgccactgt attttttccg cttgcaagat cccaaagatc caagttcagt cgacctatgg
      241 cgtttttcat tatccgatat aaggcggcaa cgtggtggta ttacgatatt gaataatgtt
      301 ataaactcac tcaaaaaaga agaaaccatt gttgaacttg atattaaaaa agctggaaat
      361 gttactgttc atgtccttac cctcgatggc aatattgtaa aaaccttaca acgcgggagg
      421 ctcaatcctg gcttgcatta ttttaagtgg aatggcacaa atctcagcgg cacccctgtc
      481 gcgcgcggta tttattttat gcgtgtcgtt ggaccagata tcgatgaaac acgaaaagtt
      541 atggttataa aagagtaggc tagcatagat tctggacaaa aaagcttgtt ttgactagac
      601 tatgtgcatg tttgaagtta gagttgaagc ggactttgct gccgcacatt ttttacagga
      661 ttttcacggg aagtgcgaaa gactacatgg tcataattat caggtatttg cacatgcccg
      721 tggaacagac ttggacgaag gtggcatgct tttagacttt ggcattttga aaaaagagct
      781 acgttctatt tgtaaggaac ttgatcacag ccacttaaat gaaaatgcgt tttttgaaaa
      841 caatccttct gcagagcgca tcgccttgta tatttttcaa aaacttgaaa gcgcattgca
      901 ggagtatagc ccaaaaacag ccgccttgct ctatgcagtt gatgtttttg aaaccgcgac
      961 gagtcgcgca cgttatacaa aagactaagt ttttatagaa cctgaatacc acgttttttt
     1021 agctcagcct ttatctcccc cacggtatgg atttttaggt gagcgatact tgcaatgagg
     1081 gctgcgcttg cacttccttt gctaagcact tctgcaatat gctcgatact accgccgcca
     1141 ccggaagcca caacgggaac tgaaaccttt tctgcaatga gctttgtcag ctctatgtca
     1201 taccccgcct ttgttccatc agcatcgata gagtttaaga caatttcccc tgcaccaaga
     1261 gctaccgcct tttcagccca ttcgagcacg tcgaggtctg ttggcgtttt tcctccgccg
     1321 agcataacac ggtaaccatt tttacagctt gcgtcttttt ttgcatcgat gcccaaaacg
     1381 agactttcac ttccaaaatt ttttgctgcc tctgctaaga gatctggatt cctaacagca
     1441 ggcgaattta tgcttatttt atcaactccg cagtctttga gtttttttat atcttcaata
     1501 cttgccacac caccgccaac acagagtgga acgcttgttt tttctcgaac cctttgtata
     1561 agagggaagt ttggcccctt tcccaatgcc gaagccataa tatcataaaa aacaagctca
     1621 tcgacagcct gtttcgagta gttttctgcc atttctacag ggtcgccgag agaaataaca
     1681 ttttcaaact gcacgccctt gctcactttt ccatctttaa tgtctaaaca aataataatt
     1741 ttttttggat cgtttttcat attaaaataa tattcctatg tacaaaactg gtcaagagct
     1801 tttccaaaaa aaccgataca ataagcatgg atacagaaaa tctttttgca cttgttttaa
     1861 acgttataaa aaacaaatac atcattttta tatttatagt ggtattttta tatctatctt
     1921 ttttcctata tgtggtcaat tatagaaaaa agccacgtaa aataaaggtg aaaaagctaa
     1981 aagaaccagc tccacccaaa ccttcaagcg aagaagagga agatgaagtc gatgatgaag
     2041 ctacttagct ttgtacgata aaagtcttat ttgccgaaac aactttctac cattttcatt
     2101 gatttgatta taactcgtat caataatctg atagggattt ataagtgtcg ggattttata
     2161 cgcataccaa atattttgtc ccggcatata gaaagcatca tttcgcaatt tttcaggttg
     2221 gagatttgct ggtccaacat aactttcctg cacaaattct aagtctagcc tatcgccact
     2281 tctgtcaaag ctgagcatat agtttacaaa attatttcgc atatcataga gttcgtatga
     2341 ataatccatg aaaagcgaga ctttttgtac atagcgagta attgttcctt cttccttcca
     2401 gtaaataaac tgatagtttt tatcgccatc tgtataattt gattttggat tcgtgtattc
     2461 gtttttagat acttcactaa cgaaaatttg cttatctcca tcttcgctta tagtcgtttg
     2521 cctataatag gtctctagct tttctatttc ttttacagga ttttctatag tttcaatggc
     2581 agtattaaac ttcggacctg gtatgagaac aagatcatag ctagaataat tttttttcaa
     2641 attcgtcgat tgtgatccaa gagttgaaat aatcgaactt aaaaaagagg catgattcgt
     2701 cgttgcatag ggagtataaa taaaatcaat atgtacaaat tttggaccct ctgtcgtatt
     2761 tgcaaagtta ttttcaagcg cctcgcgaat ttcatcgtca tggataaagt caggatttgc
     2821 accagagaag ataagagcca cagtgatagt taatttttcc ttcgtctcat tgagtttttc
     2881 ctctgcaaga ctcgggtagt atgcaaaggc ttcttcatag ataagatagg cttttcgtaa
     2941 tcggctataa tcttcgatgc tcaaaagaga gtccgcctca gagataagaa tagtagctat
     3001 ttctgggagc atattatttg ctctgtctct tgcatctgca taatgagcta tccaagaatc
     3061 tgcttctaga aaatatgcgt gagcattttt taagctgtca gatgtttttg tttcgtaaag
     3121 cttgagagcg gcctcatagg aaagagaagc accgagtgtt tgcgtttctt ctaaaagatg
     3181 atttgagtag ctaagcgatt tatgcaagta ttcaacggcg cttatcgttt catttaaact
     3241 atggggtctt tctgcaatag aacgcgcttg cgtaaagtat ttttctgctg tgtatttgtt
     3301 tgcggtttct tttaacggct tatagtcaat atagctaaaa aatgcgcttt gtttttttcc
     3361 gtaaagccca cccggatagc gactttctaa gatagctata agttcataga gctgcaacca
     3421 atcatcgatc gagtctgcaa ctacttcata ggcttctgga tttagattta cccgagttat
     3481 tttttcattg atatcggctt ttgccttatc catttctata ttgaattgtt tgatataagc
     3541 atcacccgtc gagcctttta gcaaaagctg tgcggcgcta taagctcttt ggataggatt
     3601 tgccctacgg attttactac tcgcacaaga gctgaggctt ataaggataa ggagaaaaac
     3661 gctaaaaacg atagatattc ttttcatact tttattttat cctatgtttt cttttttatc
     3721 tagttttgat tttgtttttt ttgcgttttg ctatgcttat tttacctttc caggggtttg
     3781 gggagataga aattttcttt tcaaataaaa gattttgaat aatttctcgg atatcagcgc
     3841 tttcccagat tctcatttta tatttttgag ctgccttttc gttacaggtt ttgacaatcg
     3901 tatcagtggc ctcttccata tcataacact
//
LOCUS       DAOSSW010000110         3928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_20792_length_3928_cov_5.728376, whole genome shotgun sequence.
ACCESSION   DAOSSW010000110 DAOSSW010000000
VERSION     DAOSSW010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20792_length_3928_cov_5.728376"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PKW26_07725"
     CDS             complement(<1..653)
                     /locus_tag="PKW26_07725"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOQ93493.1"
                     /translation="MKKKQNIPFSKPAIGKEEKKAVQRVLNSSWLTTGKEALAFEEEF
                     CFLLNVKHSLTVNSATSGLHLAFEACGIKEGTKIITSPYTFISTASPAIHLGAEIAYC
                     DIEKDSYNIDPEKIEDILKKDIHQKKIKAIVPIHIAGNPCNMKDIRFLANKYGVYIIE
                     DAAHALPSITDDGWLGTLGDIGVFSFYATKTITTGEGGMVVTKNDELAQRMKTMRLHG
                     "
     gene            complement(650..2128)
                     /locus_tag="PKW26_07730"
     CDS             complement(650..2128)
                     /locus_tag="PKW26_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOQ93494.1"
                     /translation="MPQTYKKGIYIIGAGFAGRMLSEEIQKKKLFGQLIAFLDDNPEK
                     IGKTIDGVPVLGPIAHFSSVLRHSSRDEAIIAIPSAPKERIAEIYDVLAKAQFSRIRI
                     LPSVSQIIDGDAHLIQTREINPLDILGRNPVRISLKESLGYLRGKRVLITGAGGSIGS
                     ELARQLLSGGASRLYLFGHGENSIYLIDKELRILQKEGVGEAATIIPVIGELKDRDYM
                     FWIMKNLKCDVVFHCAAYKHVPLMEENPVAVIENNVFGTKNLLDASLKANVERFVLIS
                     TDKAVKPHCIYGASKHLCENLLIDAAKKAKKDQAFMFVRFGNVMGSRGSILPLFSAQL
                     ASGGPLTVTDPEVKRFFMTIPEASSLVLQAGGVGKNEGAYLLDMGEAIKILDIAEEVI
                     RIQGYEPYKDVQIEFIGLRKGERMEEFLWTDAEEPEKTKYEKLLSLKPVAKASELTKL
                     LDLLEPICMYKADKPELYRNQNALYKILKTHFQNLDIKIGVL"
     gene            complement(2112..2654)
                     /gene="loaP"
                     /locus_tag="PKW26_07735"
     CDS             complement(2112..2654)
                     /gene="loaP"
                     /locus_tag="PKW26_07735"
                     /inference="COORDINATES: protein motif:HMM:NF033641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator LoaP"
                     /protein_id="HOQ93495.1"
                     /translation="MNYYIIQVKSLKEDEYIQRIEKRLEFRSKSQAFIFPKRELKIKK
                     NGKTTKQTLPLFSSYIFIQTEEVDRQLYNIMKTTPYFYRFLPNNQSIQKLEGKDLSLI
                     QHFLQFGKTIESSRVIFDENERIIVKEGPMKGLEGLITKVDRRKKRARIQVELAQTPI
                     IFDLSFDVIEKGGENNATNL"
     gene            2835..3305
                     /locus_tag="PKW26_07740"
     CDS             2835..3305
                     /locus_tag="PKW26_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93496.1"
                     /translation="MKRISMCFLASFFFLSLGFGNSITILVNQKTSNAEGIFESSRLF
                     EDGVINYFFDSGFIVTNEPVCLEKDFLRAEQIALDEAQIGYLEFLLVFHLYLNAENGN
                     LTEAEWDLIRVETGKKIASGKSLAPEVKTKTETEKITKQFAEQNVREVLKYVRK"
     gene            3316..>3928
                     /locus_tag="PKW26_07745"
     CDS             3316..>3928
                     /locus_tag="PKW26_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93497.1"
                     /translation="MKKIIQAFILICLISLPTIAQIHNPSLDGRAIVAPKGSYPSGLY
                     GKAPGFLPGDTLIVTSYTNGFSIEVLILSSIDTSSGIAIELSEEAAQALEISRNTDSY
                     VKIQKKSAYQKEVIKTPAQDIHEELALDPDKTPSLALKQNEEIERYIEDIKATYATED
                     SPLLIVDSSEVVFDDQNDVDENSLAKSEDLVFETNDLENTRIKD"
BASE COUNT         1182 a          736 c          725 g         1285 t
ORIGIN      
        1 ccgtgcaggc gcattgtttt catacgctgt gcaagctcgt cattttttgt aacgaccata
       61 ccgccttccc cagtcgttat cgtttttgtt gcataaaaag aaaaaacccc gatatcgccg
      121 agtgtcccca gccagccatc atcagtgatg gaaggaaggg catgagctgc atcttctata
      181 atatagactc catatttatt tgcaagaaag cggatatctt tcatgttaca agggtttcct
      241 gcgatatgaa taggaactat cgcttttatt tttttttgat gtatatcttt ttttaaaata
      301 tcttcaatct tttctgggtc aatattgtaa gagtcttttt ctatatcaca gtatgctatt
      361 tcagcgccga ggtgaattgc aggcgatgct gtcgaaataa aggtgtaggg tgaggtgatt
      421 atttttgttc cctcttttat accacaggct tcaaaggcga gatgcaaacc gcttgttgca
      481 gaatttaccg taaggctatg tttgacattt agcaaaaagc aaaattcttc ttcaaaggca
      541 agggcttctt tccccgttgt aagccaacta gaattgagca ctctttgtac cgcttttttt
      601 tcttcttttc ctattgcggg ttttgaaaaa ggaatgtttt gctttttttt cataatacac
      661 ctatttttat atccaagttt tgaaaatgcg tttttaatat tttatacaag gcattttgat
      721 ttctatacag ttcgggctta tctgctttat acatacaaat gggttcgagc aagtctaaaa
      781 gttttgtaag ctcacttgct ttagcaacag gcttcaaaga taaaagcttt tcgtattttg
      841 ttttttctgg ctcttctgca tccgtccata aaaactcttc cattcgctct cccttacgta
      901 agccaataaa ttctatttgc acatctttgt agggttcgta gccttgtatg cgaataactt
      961 cttctgcaat gtcgaggatt tttattgcct ctcccatatc caagagatag gcaccttcgt
     1021 ttttgccaac gccaccagcc tgtaaaacaa gggaagaggc ttcagggatt gtcataaaaa
     1081 agcgttttac ttcaggatcg gttactgtaa gtggtccacc agaagcaagt tgtgcagaaa
     1141 aaagcggtaa aatggaacca cgggaaccca tgacattgcc aaaacggaca aacataaagg
     1201 cttggtcctt ttttgctttt tttgcggcat caattaaaag gttttcacaa agatgtttgg
     1261 aagctccgta aatacaatgc ggtttaaccg ccttatcggt agaaatgaga acaaatcgct
     1321 ctacatttgc tttaagcgaa gcatctaaaa gattttttgt tccaaagaca ttgttttcta
     1381 taaccgctac ggggttttct tccataaggg gaacatgctt atatgccgca caatggaaaa
     1441 caacatcgca tttgaggttt ttcattatcc aaaacatata gtctctgtct tttaactcac
     1501 caataacggg aataatcgtc gcagcttcac caacgccttc tttttgtaaa atacgcaatt
     1561 ctttatcgat gagatagatg gagttttccc catggccaaa gagatagagc cgagatgcac
     1621 cgccggagag taattgccgt gcaagttcag agccaattga gccaccggca cccgtaatca
     1681 aaacccgctt cccacgcaaa tagccgaggc tttcttttaa ggaaatgcgc actggatttc
     1741 tcccgagaat atcgagtgga ttgatctcgc gcgtttgaat aaggtgtgca tcgccatcga
     1801 ttatttgtga tacgctcggc agaatgcgta tgcgagaaaa ttgcgccttc gctaaaacat
     1861 cgtatatttc tgctattctt tcttttgggg cacttggaat agctataatc gcctcatcgc
     1921 gagagctatg tcgaagaact gatgaaaaat gcgcaatagg acccaaaaca ggaacgccat
     1981 caatggtttt gccaattttt tcaggattat catctaaaaa agcaattagc tgcccaaaaa
     2041 gctttttctt ttgtatttct tcagaaagca ttctgccggc aaaacctgca ccaattatat
     2101 atattccctt tttataagtt tgtggcatta ttttccccac ctttttcgat aacatcgaag
     2161 gataaatcaa agattatggg cgtttgtgcc aactcaactt gtatacgcgc tctttttttt
     2221 cttctatcaa ccttggttat caaaccttct aaacccttca taggcccctc ttttacgata
     2281 atacgctcgt tttcatcaaa aataaccctc gaagattcta ttgtttttcc gaattgcaaa
     2341 aaatgctgta taagactgag gtccttaccc tcaagttttt gtattgattg attattgggt
     2401 aaaaatcgat aaaaataggg cgttgttttc attatattgt agagttgtcg gtcaacttct
     2461 tctgtttgaa taaatatata acttgaaaaa agaggaagag tttgttttgt tgttttccca
     2521 ttttttttaa ttttcagctc acgctttgga aagataaaag cttgagactt gcttcgaaat
     2581 tctaaacgtt tttctatacg ctgtatatat tcgtcttctt tcaaagactt cacttgaata
     2641 atataataat tcatatgcga acatagtatc atatgatgcg tctttcttca ataaagatta
     2701 aagagtccaa aaagatttta agatgggctg tgtttttcac atgtttttcc ctaatattta
     2761 tacttaaact tccgatgcta aatataagac tgttgtaaca ttttgtcttg tcagtctttt
     2821 tataaggatt ttttatgaaa cgaatatcta tgtgctttct tgcatctttt ttctttttat
     2881 ctcttggctt tggaaattca attactattt tagtgaatca aaagacgagc aacgcagaag
     2941 gaatttttga atcaagtcgt ttatttgaag acggcgttat aaattatttt ttcgacagtg
     3001 gctttattgt aacgaatgaa ccagtttgtc ttgaaaaaga ctttttgaga gcagaacaaa
     3061 ttgccctcga tgaagcacaa ataggatatt tagaattttt acttgtattt catttatatc
     3121 tcaatgcaga aaatggaaat ctaacagaag ctgaatggga tttaatacga gttgaaacag
     3181 ggaaaaaaat agcatcaggt aaatctcttg cgcctgaggt aaaaacaaag acagagaccg
     3241 agaaaataac aaaacaattt gcggaacaaa atgttcgtga agttctcaaa tatgttcgta
     3301 aataaaggtg ggaaaatgaa aaaaataatt caagcgttta tcttaatttg ccttatctct
     3361 ctccccacaa tagcacaaat tcataaccct tcattggatg gacgagctat tgttgcgccg
     3421 aagggttctt atccgagtgg tttgtatggc aaggcgccag ggtttttacc aggggatacc
     3481 cttattgtta cgagttatac gaatggtttt tctatagaag tgcttattct ttcaagtata
     3541 gacacttctt ctggcatagc gattgaactt tctgaagaag ctgcccaagc cttagaaata
     3601 agcagaaata cagatagcta tgtgaaaatt caaaagaaaa gtgcctatca aaaagaagtt
     3661 attaaaaccc ctgcacaaga tattcatgag gaattggcac tagacccaga taaaacaccc
     3721 tcacttgctt taaaacaaaa tgaagaaatc gaacggtata ttgaagatat aaaagcgact
     3781 tatgctacag aagattcgcc tctattaatt gtcgatagtt cagaagttgt ttttgatgac
     3841 caaaatgatg tagatgaaaa ctctcttgca aaatcagagg accttgtgtt tgaaacaaac
     3901 gacttagaaa atacaaggat aaaagatg
//
LOCUS       DAOSSW010000111         3893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_21013_length_3893_cov_6.973945, whole genome shotgun sequence.
ACCESSION   DAOSSW010000111 DAOSSW010000000
VERSION     DAOSSW010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21013_length_3893_cov_6.973945"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKW26_07750"
     CDS             <1..309
                     /locus_tag="PKW26_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOQ93498.1"
                     /translation="VAKQEHFVEFLLSTLSQLFFDAFLIALPIVGTLFLITVTMGILS
                     KAAPQMNLLSEGLPITILTAFFLLTILLPSMIDFFIQAFERAFYKLEQLLIVVGGVQL
                     "
     gene            306..1508
                     /gene="flhB"
                     /locus_tag="PKW26_07755"
     CDS             306..1508
                     /gene="flhB"
                     /locus_tag="PKW26_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOQ93499.1"
                     /translation="MRSVQERCMDFFLPGKKTRRVFAREAFARQDLQGIDLDYFDLQW
                     FASAEEEGRTEKASEHKLRKAREEGRVAKSQEISAALVMLFPVIALVILAPWYLKKFI
                     EILRFFFDRCTSPEVDLGSFAYMFFRSMITMILPLGIIAMISGVLANIVQNRGFIFST
                     KPISPKFSKILPKFGEYFKKTLFSGQGAFNVLKSIVKVAFLFTMAFILIKNDLPKLLT
                     LSNVNMWQGISHVAGMVAKLLIIAAVFFLVIAIPDYLVQRHQFMESMKMSKQEVKEEY
                     KNLEGDPFVKGRIKQYIRDLMSQNMSQHVAEADVIITNPTHYAIAIKYDREIMQGPML
                     TAKGVDSVAHRIKEIAKEHDVEIIENKPLARALYAEVQIGDIIPEAYYQVLAIILGQV
                     YKMRAEKV"
     gene            1531..3606
                     /gene="flhA"
                     /locus_tag="PKW26_07760"
     CDS             1531..3606
                     /gene="flhA"
                     /locus_tag="PKW26_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOQ93500.1"
                     /translation="MLKNLDFKNNADFVIAIAVIIVVLMLIIPLPTVLLDVLMAANLT
                     LSLLIVLIVIYTPRAIDFSSFPSVLLISTIFGLGINVSSTRLILAKGANFDGKMVRAF
                     ATFVTGAGGTEGVAIGFVIFIILIAVQTFVITKGATRVAEVAARFTLDGMPIKQASIE
                     QEYNSGLITEEEQRKKKLELQQESDFYGSMDGSTKFVSGNVKVGIFITVINLIVGMVF
                     GMVLHKEPFDVALATYTSLTIGDGLLSQLPSLLVSFATGLIVTRSASDGRTTIGSNVK
                     KEFTQSAWIYIIAGGALAALGIIPGFPWYILIPMGALIFYLGFRLLQIEKKKEKTAEE
                     EIEKKKAPAGPSGISPVVPLDALSLELGYALIPLVDEEKGGDLRERVGRIRRELGLDL
                     GLVLPPIKMIDNSILEPNEYCFKIRGVEVGRASVRMNWYMCMNTGSVTEEIEGEKTTD
                     PAFGLPAIWLTEDQRDRAQRAGYAVVDSPTVLVTHLTEMIKSRAQEILGRQEVQSILD
                     TLKKDFSAVVEEANKHFTVGEIQKVMQGLLREQVSIRNTVSILETMCDYAAITKKTDV
                     LVEKVRQRLGRQICLQYADEKKTIHVLTIEPYFVEKLDASRVETINGPIAALEPSDQR
                     NWISALSASIVAVQEKGYLPIILAPEAVRILIKSSTEREMPNLVVLSVPEIDKEIRIE
                     SLGEIKVGS"
     gene            3596..>3893
                     /locus_tag="PKW26_07765"
     CDS             3596..>3893
                     /locus_tag="PKW26_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93501.1"
                     /translation="MDLKNDIVLSLRGKTEEDCIKKLNKKYDKNYEIMSRKAVTLSSF
                     LGLIKTSGVELRYIEKEKPRFDFLEALNRQNEYQQRMQSPVIPQSPKQEQSANRR"
BASE COUNT         1157 a          736 c          827 g         1173 t
ORIGIN      
        1 gtcgcaaaac aagagcattt tgtggagttt ttactttcga cattgtctca gctttttttc
       61 gatgcttttt taatagcact tcccatcgta ggcacacttt ttttaattac cgttaccatg
      121 gggattcttt caaaggctgc accgcagatg aaccttttat ccgaagggct tcctattacg
      181 attttaaccg catttttttt attgactatt ttactcccct ctatgataga tttttttata
      241 caagcctttg agcgcgcttt ttataaactt gaacagcttt tgattgttgt gggaggggtg
      301 caattatgag aagtgttcaa gaacgatgta tggatttctt cttgcctggg aaaaaaacaa
      361 ggcgtgtttt tgctagagag gcatttgcaa ggcaagattt acagggcatt gacctagact
      421 atttcgactt gcagtggttt gcctcagcag aagaagaggg gcgaacagaa aaagcctcag
      481 aacataaact acgcaaagca cgtgaagaag ggcgtgttgc aaaaagccaa gagataagcg
      541 ccgcactcgt gatgcttttt ccggttatag cgcttgtgat tttagcaccc tggtatttga
      601 aaaaatttat tgaaatcctc cgttttttct tcgatcgctg cacaagtcct gaagtagacc
      661 tcggcagctt tgcctatatg ttttttcgat cgatgattac gatgatttta ccccttggga
      721 taattgcgat gatttctggc gttttggcaa atatcgtgca aaaccgtggc tttatttttt
      781 caacaaaacc tattagtcca aagttttcaa agattttacc caagtttggg gaatacttta
      841 aaaaaacact tttttctgga caaggtgctt ttaatgtctt aaaatctatt gtaaaggttg
      901 cttttttatt taccatggca tttattttaa taaaaaatga cttgccaaaa cttttaacgc
      961 tttcaaatgt aaatatgtgg cagggaataa gccatgttgc tggaatggtt gcaaaacttt
     1021 tgataatagc ggctgttttt ttcctcgtca ttgctattcc ggattatctt gtacaaaggc
     1081 atcagtttat ggaatcgatg aagatgtcta agcaagaagt taaggaagaa tataaaaatt
     1141 tggaagggga tccctttgta aaaggtcgta ttaagcaata tattcgagac cttatgagtc
     1201 aaaatatgag tcaacatgtt gcagaagcgg atgtaattat tacaaacccg acgcattatg
     1261 caattgctat aaaatatgat agagaaatca tgcagggacc tatgctcacc gctaagggtg
     1321 tcgactcggt agcacatcgc ataaaagaaa ttgcaaagga acatgacgta gaaattatag
     1381 aaaacaaacc tttagcacgt gcgctctatg cggaagtgca aattggtgat ataatacctg
     1441 aagcgtatta tcaagtattg gctatcattc ttggacaagt ctataagatg agggctgaaa
     1501 aagtgtagtg tattttatgg gtggggactc gtgttaaaaa atttagattt taaaaataat
     1561 gctgactttg ttatagccat tgcggttatc attgtcgttt tgatgctcat tataccattg
     1621 ccaaccgtct tactcgatgt gcttatggcg gcgaacctaa cactttcact tctcatcgtt
     1681 cttattgtta tttatacccc acgtgctatc gatttttcaa gttttccttc tgttttgctt
     1741 atatctacta tttttggtct tggcataaat gtttcttcta ctcgtcttat tcttgcaaaa
     1801 ggtgctaatt ttgacggaaa aatggttcgt gcctttgcga cttttgttac aggagcaggg
     1861 ggaactgagg gtgtcgctat tggctttgtt atttttatca tactgattgc ggtgcaaacc
     1921 tttgttataa ccaagggtgc gacgcgggtt gctgaagtag ccgcacgctt taccctcgat
     1981 ggtatgccga ttaagcaagc gagtatagaa caagaataca attctgggct tataacagaa
     2041 gaagagcaac gaaaaaagaa gcttgagtta caacaagaat ctgactttta tggttccatg
     2101 gatggatcga caaaatttgt ctctggaaac gtaaaggtgg gcatttttat cactgttata
     2161 aacctcatcg tcggtatggt ttttggcatg gttttacaca aagagccctt cgatgttgca
     2221 cttgctacct atacttcttt gacgattggc gacgggcttt tgtcgcagct tccttcctta
     2281 cttgtttcct ttgcaacagg tttaattgtt acgcgctctg catccgatgg gcgaacaacc
     2341 attggctcaa atgtaaaaaa agaatttacg cagagtgcat ggatatatat tatcgcaggc
     2401 ggtgctcttg ccgcactcgg tatcatacct gggtttcctt ggtatatcct cattcccatg
     2461 ggcgctctta ttttttatct cggtttccga ttgttacaga tagagaagaa aaaagaaaaa
     2521 accgccgaag aagaaataga aaagaaaaaa gcccccgcag gtccttcagg cataagtccc
     2581 gttgttcctc ttgacgcgct ttctcttgaa cttggctatg ccctcatccc actggttgac
     2641 gaagaaaaag gcggagactt gcgagagcgt gtcgggcgca ttcgtcgcga actcggcctc
     2701 gatcttggac tcgttttacc accaataaaa atgatagaca atagtattct tgagccaaat
     2761 gagtactgtt ttaaaattcg tggagtagag gttggacggg caagcgttcg tatgaactgg
     2821 tatatgtgca tgaacactgg atctgttaca gaagaaattg aaggtgaaaa aaccaccgac
     2881 ccagcctttg ggcttcctgc tatttggctt actgaggatc aaagagatag ggcacagcgc
     2941 gcaggctatg ccgttgtcga ctctcccaca gttttggtaa ctcaccttac cgagatgata
     3001 aaatcacgcg cacaagaaat tctcggtcgc caagaagtac agtcaatttt ggatacactc
     3061 aaaaaagact tttcagccgt tgttgaagaa gcgaacaaac actttaccgt tggtgaaatt
     3121 caaaaagtta tgcaaggact tttacgcgag caagtttcta ttcgaaatac tgtttctatc
     3181 ttggagacaa tgtgcgatta cgcagctatt accaaaaaaa cagatgtgct tgttgaaaaa
     3241 gtgcgacaaa gactcggcag gcaaatttgt ctacaatatg ctgatgaaaa gaaaacaata
     3301 cacgttctaa ccatcgaacc ctactttgta gaaaaactcg atgcaagccg tgtagaaaca
     3361 ataaacggac ctattgctgc cttagagccg agcgatcaaa gaaactggat atcggcctta
     3421 agtgcttcaa tagtggcggt gcaagaaaaa ggctacttgc ccattatcct tgctcctgaa
     3481 gctgtccgta ttttaataaa gtcgagtact gaacgtgaaa tgcccaacct tgttgtgctt
     3541 tctgtaccag aaatagataa agaaattcgt attgaatcct taggagaaat taaagttgga
     3601 tcttaaaaat gacattgtgc tttctcttcg aggcaaaaca gaagaagact gcataaaaaa
     3661 actgaacaaa aagtatgata aaaattatga aataatgtct cgtaaagcgg taacgctttc
     3721 atctttttta ggcctcataa aaacctcagg tgtagaatta cgctatattg aaaaagaaaa
     3781 acctcgcttt gatttccttg aagccttgaa caggcaaaat gaatatcaac agcgaatgca
     3841 aagtccggtg ataccacaaa gtccaaagca agaacaaagt gcaaataggc gat
//
LOCUS       DAOSSW010000112         3813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_21578_length_3813_cov_6.379457, whole genome shotgun sequence.
ACCESSION   DAOSSW010000112 DAOSSW010000000
VERSION     DAOSSW010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21578_length_3813_cov_6.379457"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PKW26_07770"
     CDS             complement(<1..515)
                     /locus_tag="PKW26_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOQ93502.1"
                     /translation="MQHLKPLPGSEIAKKNSSIVPKNGDIRRVLGVDPGLASTGWAVV
                     DFYKNRYACIAYGVIETKPSQIHGERLLMIFDKIEEIIQSYKPREAAMETLYFSKNVS
                     SAMKVSEARGVVHLCLSKNHLICGEYTPLIIKKTITGSQKAKKTDIQDYLKLLLGLQE
                     KIEPDHASDALA"
     gene            complement(519..1259)
                     /locus_tag="PKW26_07775"
     CDS             complement(519..1259)
                     /locus_tag="PKW26_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ93503.1"
                     /translation="MSGHSKWATIKHAKGAADAKRGQLFTKFIKEISIAAKMGGGDPQ
                     ANPRLRTAILKARGANMPKDNIERAIKKGTGELGGEVYEELSYEGYGPGGVAILVEVL
                     TDNKNRSAANIRTIFAKTGGNLGTTGSVSYMFNRKGVIDYDSEVVSEDEIMEFALEAG
                     ADDIVNEDGIISVYTAPENFETVLEKLQEKNFESVSAEISMVPDTYTALDTETTRKVL
                     KLIERLEEDDDVQNVYSNIDIPEGFEAE"
     gene            1412..2506
                     /locus_tag="PKW26_07780"
     CDS             1412..2506
                     /locus_tag="PKW26_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOQ93504.1"
                     /translation="MGKTTFIIAEIGTSHCTSLKKAKALVDAAKKSGASAVKFQIVYA
                     DEILHPKTGFVRLPTGNIPLYERFKELEVPLSFYEELRAYSYEKGLLFGASPFGLKSL
                     EELCRLNPDFIKIASPELNHYPLLEAVSKINIPVILSSGVSLLGDIEKALFYFQDRKD
                     RVSLLHCITQYPAPEDEYNLSLLPSLKNIFGVKVGVSDHSLNPILVPVLSYLQGAEII
                     EKHICLDKTEEGLDDPVALSPNEFKTMVDAIRMAEKAPKKMYKKCIKTYGKSYVKAVL
                     GTGEKKLAPSEKAAYTKTNRSLHYMRDMEEGEIIQEKDISILRTEKILTVGESPEFLS
                     LFVGSRLQTKVISGEGALIEQLIAKGNHEK"
     gene            2496..>3813
                     /locus_tag="PKW26_07785"
     CDS             2496..>3813
                     /locus_tag="PKW26_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93505.1"
                     /translation="MKNKTFHIIFFFLCLTLPLLADTEISLIKNDEKLYKIQNVFYDS
                     QNAFHEKTLLKKIPIDRNHFFKEEELLDYIAKLEKQFLDTRLIDAVKIDYAIAKKTEN
                     TSESDNTNLVLIDFFIQIQKSGNFIIFPYPKYDSNTGFSLKAKLKNYNAFKRMEIFSS
                     DLSYSLTPENTTALESEIDFNLPFYLGYTNNKVYSDVDLFYDVDKIFFQSDSKLGIEF
                     SFPIEKISLNVFFEEQYIVKDEAHLNQALGLFFPLLFGKEIEFILKPGFMYLYSLALE
                     GSQTSSSYLIPSFDIETKKNSYTGNFRNGYEVSSETNFDFHLEKKRFNPNLLLQAQFF
                     TPISKKDEPFFLHSTRLIYYYNQERDIPMGLFIRGILDTELMAKHALVINNDFTFKLF
                     NTRFNTKHSSKFNFELQTSLFFDTSFIFAPEFIFDQTMGLNLIVQPE"
BASE COUNT         1207 a          759 c          598 g         1249 t
ORIGIN      
        1 gcaagggcgt cgcttgcgtg atcaggctct atcttctctt gcaagcctaa aagtagttta
       61 agatagtctt gtatatctgt tttttttgct ttttgactgc ctgttattgt tttttttata
      121 atcaggggcg tatattcccc acaaatgaga tggttttttg aaaggcaaag gtggacaact
      181 cccctcgcct cagaaacctt catcgctgaa ctcacatttt tagaaaaata gagcgtttcc
      241 attgccgctt ctcttggctt atagctttgt attatttctt ctattttatc aaaaatcata
      301 agtaagcgct cgccatgtat ttgacttggc tttgtttcga taacaccgta agcgatacat
      361 gcgtatcggt ttttatagaa atccactaca gcccaacctg ttgaagcaag gccagggtca
      421 acacctaaaa ctcttcgaat gtcaccattt ttcggtacaa tcgaagagtt tttttttgct
      481 atttcactcc caggtaaggg ttttaagtgc tgcatcagct attcagcttc aaaaccttct
      541 ggaatgtcga tatttgaata tacattttgc acatcatcgt cttcttcaag cctttctata
      601 agtttcaaaa cttttcgtgt tgtttctgta tccagggcgg tataagtatc tggaaccatt
      661 gaaatttctg cagaaacaga ttcaaagttt ttctcttgta atttttctag cactgtttca
      721 aagttttctg gtgcagtata aaccgaaata atgccatctt cgtttacaat gtcatccgcg
      781 ccggcttcaa gagcaaattc cataatctcg tcttcactga caacttcaga gtcgtagtca
      841 ataacacctt ttctattaaa catataagaa acagaacctg ttgttcctag gtttccacca
      901 gtttttgcaa aaatcgttcg gatatttgct gcagatcgat ttttattgtc tgttaatact
      961 tctacaagaa ttgcaactcc accgggtcca taaccttcgt agctaagctc ttcataaact
     1021 tcaccaccga gttcacccgt accttttttt attgcccttt caatattgtc cttgggcatg
     1081 ttagcaccac gtgcttttag aatagctgtt cttaaacgag ggtttgcctg tggatcgccg
     1141 ccacccattt tcgctgcgat agaaatttct ttaataaact tggtaaataa ctgacctcgt
     1201 tttgcatcag ctgcaccttt tgcgtgctta attgtcgccc atttactatg tcctgacatt
     1261 tttgctccta atagtatcat tgattaaacc ttgcaacgca aggcaaaaga atataataat
     1321 tatctttcaa aataatgata aaatgattta atcaatttta ccactttaaa ggcttatagt
     1381 caatgaagct attttttagt atacttaaaa catgggcaaa acaacattca ttatagcaga
     1441 aataggaaca tcacattgta catctctgaa aaaggccaaa gctcttgtag atgctgcaaa
     1501 aaaaagcggt gcaagtgctg taaaatttca aattgtctat gctgatgaaa tattgcaccc
     1561 caagactggc tttgttcgct tgcccactgg aaacattcct ctatacgagc gatttaaaga
     1621 gcttgaggtc ccgctttcct tttatgaaga actacgtgcc tatagctacg aaaaaggact
     1681 tctctttggc gcttctccct ttggactcaa aagcttagaa gaactttgcc gcttaaatcc
     1741 tgattttata aaaattgcat ccccagaact caatcactat cctcttcttg aagctgtttc
     1801 aaaaataaat attcccgtca tcctctcttc cggagtctcc cttttgggcg atatagaaaa
     1861 agctcttttt tacttccaag atagaaagga cagagtcagt cttcttcatt gcataacaca
     1921 ataccctgca cctgaagatg aatataatct gtctttactt ccatccttaa aaaatatttt
     1981 tggagtgaaa gttggtgttt cagatcacag tctaaatccc attttagtac cggttctttc
     2041 ttacttgcag ggggcagaaa ttatagaaaa acatatttgc ctcgataaaa ccgaagaagg
     2101 acttgatgac cctgtcgcac tttctccaaa tgaatttaaa accatggtcg atgcaattcg
     2161 aatggctgaa aaagctccta aaaaaatgta taaaaaatgt ataaaaacct atggtaaatc
     2221 ctatgtaaaa gctgttttag gtacaggcga aaaaaaactt gctccctccg aaaaggcagc
     2281 ctatacaaaa acaaatcgaa gtttgcatta tatgcgcgat atggaagaag gagaaattat
     2341 ccaagaaaaa gacattagta tactccgcac agaaaaaatc ttgactgtag gtgaaagtcc
     2401 tgagttttta tctctctttg ttggatcgcg tttgcaaaca aaggtcatat cgggagaagg
     2461 cgctttaatt gaacagctta ttgcaaaagg taatcatgaa aaataaaact tttcacatca
     2521 tatttttctt tttatgctta actcttcccc tcctagcaga cacggaaatc agcttaataa
     2581 aaaatgatga gaaattatat aaaatacaaa atgtttttta tgattctcaa aatgccttcc
     2641 acgaaaaaac acttctaaaa aaaatcccaa tcgatagaaa tcattttttt aaagaggaag
     2701 aacttttaga ctatattgca aaacttgaaa aacagtttct ggatacacgg cttatcgatg
     2761 cagtcaaaat tgattatgct attgcaaaaa aaacagaaaa tacaagtgag tccgataata
     2821 caaatcttgt tctcatcgat ttttttatac agatacaaaa atcgggtaac tttattattt
     2881 tcccctatcc taagtatgat tcaaatactg gtttttcatt aaaagccaaa ctaaaaaatt
     2941 ataatgcttt taaacgtatg gaaatttttt catcggacct tagctatagc ctcactccag
     3001 aaaataccac agccctagaa agcgaaatag actttaacct tccattttat cttggctata
     3061 caaacaataa agtttactct gatgtcgatt tattttacga tgtagacaaa atattttttc
     3121 aaagtgattc taaactcggt atagaatttt ctttccccat agaaaaaatc tcgctcaatg
     3181 ttttttttga agaacagtat atcgtaaaag atgaggctca tttaaaccaa gcactcggac
     3241 ttttttttcc ccttttattt ggcaaggaaa tagagtttat cctaaaacct ggatttatgt
     3301 atctgtatag cttagcttta gaaggctctc agacatcttc ttcctatctt atcccaagct
     3361 tcgatataga aacgaaaaaa aattcttata cggggaattt tcgaaatggc tatgaagtaa
     3421 gcagcgaaac aaattttgac tttcatttag aaaaaaaacg atttaatcca aaccttttac
     3481 tacaagcgca gttttttacc ccaatctcaa aaaaagatga gccatttttt cttcactcta
     3541 cacgacttat atattattac aaccaagaaa gagacatacc gatgggttta tttatccgcg
     3601 gtattcttga taccgaacta atggcaaagc atgctcttgt aataaataat gattttacat
     3661 ttaagctttt caacactcgc ttcaatacaa aacattcttc caaatttaac tttgaattgc
     3721 aaacaagcct tttctttgat acgagtttta tttttgcacc agaatttatt ttcgatcaaa
     3781 caatggggct aaacctcatt gtgcaacctg aat
//
LOCUS       DAOSSW010000113         3637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_22953_length_3637_cov_7.042434, whole genome shotgun sequence.
ACCESSION   DAOSSW010000113 DAOSSW010000000
VERSION     DAOSSW010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22953_length_3637_cov_7.042434"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1540)
                     /locus_tag="PKW26_07790"
     CDS             complement(<1..1540)
                     /locus_tag="PKW26_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93506.1"
                     /translation="MKVRPFFRKIIQLFKVLGIVVSTLFLVLLVWFVFSFLDTKKAVD
                     VLPPGFTFLLHSESFYDSVSPLLDLEALDIVVSKDSFSAMRPLLFSLRSSSLLKNPIA
                     KNMLSRKIDAAAYVYPDENKNEKDRPGDFFLAINLSHWSAFSRLSGFLSGFVKMPELK
                     YIRSQFNYFEYTLETGSVYIRPYKNLLLLSTSFDLLRESAYRAEKKEGFEGFPSLVFE
                     KEKDKIQILSDTKRILSLVSADNLLLEKVKKIIPEHSFSEISFSITDAEIELKLELPV
                     QKSIVDELKESDPVLRLVSKKQSLPLLLSRLPDTSQYYTLYNAGSLEEAKDAFVSTLK
                     NPAAFQSQWETADSMSRLLFSANLYDLLFSWTGKEAAVFGLEGKKDPVFALHIQDERQ
                     RKKIFESFLSSIIIQEGDSHIYDGVKIPSINIPTFIRSLLTAFNIDLPHPFYLVKDEF
                     IFFSNSPENLLEIYKKQNRGETLLKNTVWNSLAPRTNNNFAIGLYYDLEKTLPFFAQG
                     DSILA"
     gene            complement(1633..2991)
                     /locus_tag="PKW26_07795"
     CDS             complement(1633..2991)
                     /locus_tag="PKW26_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOQ93507.1"
                     /translation="MEINNGEEKINRASLPMLSLTIPLLFENGFRILLSSVDTFMLSS
                     WSQKAVAAAGMIGQYIFFIQILFNVICIGTSIVLSQYLGAKRNKEAEQVTQASVLMIS
                     LVALFIMLLIFFGAAPLLSLYSIEDDVRHMAWQYLVIFGGFGAFFMALNMLQGTVLRA
                     YGYTKDAMYIAIVANILNILGNAISLYGFFGLPVLGIAGVAASSAFSQLAASIILAIR
                     IRQKKDVRFPLKRWRDVSPSMYRVVLSVGVPTAGENLSYNVAQIVIMVMVSTFGTWAM
                     SAMVYTQTIARFVGIFGLSIGTATQIKVGYWVGAKKPEDAYRRVYLYQIIATTCTVVF
                     LILINIFKVPIIALFTDEKEIASMVYRLLLFSIYLEFGRSINLVTISALKGAGDFRFP
                     VLYGLFSMWGIMVLGSYLLGVKAAWGLVGIWLSIGSDETIRGFVMLFRWRSKKWITKR
                     LK"
     gene            complement(3137..3235)
                     /gene="ffs"
                     /locus_tag="PKW26_07800"
     ncRNA           complement(3137..3235)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKW26_07800"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3305..3390)
                     /locus_tag="PKW26_07805"
     tRNA            complement(3305..3390)
                     /locus_tag="PKW26_07805"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3354..3356),aa:Ser,seq:gga)
BASE COUNT         1244 a          771 c          636 g          986 t
ORIGIN      
        1 gcgccaaaat agaatcaccc tgcgcaaaaa aaggtagcgt cttttctaaa tcatagtaaa
       61 gccctatcgc aaaattatta tttgtccttg gggcaagaga attccacaca gtatttttca
      121 aaagcgtctc tcccctattt tgctttttat agatttccaa aagattttct ggcgaatttg
      181 aaaaaaagat aaactcgtct tttacaagat aaaaaggatg cggtaaatca atattaaagg
      241 cggtcaaaag gctccgtata aaagtgggga tatttatact tgggattttc accccgtcat
      301 aaatatggct atcgccttct tgaattatga tagatgaaag aaaggactca aaaattttct
      361 ttctctgcct ttcatcttga atatgtaggg caaatacagg gtcttttttt ccttcaagcc
      421 caaaaactgc agcctctttt ccagtccatg aaaaaagcaa gtcatataaa tttgcagaaa
      481 ataataatcg actcatggaa tcagctgttt cccattgcga ttgaaaggca gctggatttt
      541 taagcgttga aacaaaggca tcttttgctt cctctaaact acctgcgttg tacagagtat
      601 aatattgact tgtatcggga agacgagaaa gtaaaagcgg aagactttgt ttttttgaaa
      661 caagacgcaa aactgggtcg ctttctttca gctcatcgac tatagatttt tgcacaggca
      721 attccagttt gagctctatc tcagcatcag taattgaaaa agagatttct gaaaatgaat
      781 gctcgggtat gattttcttt accttttcca gcaagagatt gtcagcgcta actaaggaaa
      841 gaattctctt tgtatccgat aagatttgaa ttttatcttt ttctttctca aaaaccaaag
      901 aagggaagcc ttcaaagcct tctttttttt ctgcccgata agccgactca cgcaacaagt
      961 cgaagctagt actaagaagc aaaagattct tgtatggccg aatatacacg gagccagtct
     1021 cgagcgtata ctcaaaataa ttaaattgcg agcgaatata ttttaattca ggcattttca
     1081 caaatccgct caaaaatcca gaaagccgtg aaaaagcaga ccaatgcgaa agatttatag
     1141 caagaaaaaa atccccaggt ctatcttttt catttttatt ttcatcagga tagacataag
     1201 cagcagcatc gatttttctc gaaagcatat tttttgctat gggattttta agcaagctag
     1261 atgaacgcag agaaaacaaa agagggcgca ttgcagaaaa agaatcttta cttacgacaa
     1321 tatcgagcgc ttccaaatca agcaaggggc tcacagaatc ataaaaagat tcactgtgca
     1381 ataaaaaagt aaaaccggga ggcaatacgt ctactgcttt ttttgtatct aaaaagctaa
     1441 atacaaacca aacgagcaaa accaaaaaaa gagtcgatac aactatgcct aatactttaa
     1501 aaagttgtat tatttttcga aaaaagggac gaactttcat actaattcct acaaatataa
     1561 cttatgataa gaatagcata gggattagac tttgtaaacg aaaaaaaact ggaaatcaaa
     1621 gtcaaggtgt ttttatttta accgctttgt gatccatttt ttactcctcc atctaaaaag
     1681 catcacaaaa ccacgaatcg tttcatcact tccaatggaa agccaaatcc ctacaagtcc
     1741 ccaagctgct ttcaccccta aaagataact tcccaaaacc attatccccc acatggaaaa
     1801 taagccatat aaaacaggaa agcgaaaatc tcctgcaccc ttgagggcag aaatagttac
     1861 aagatttatc gagcggccaa attcaagata gatggaaaaa agtaaaagcc tataaaccat
     1921 agatgctatt tctttttcat cagtaaagag cgctattatc gggaccttga agatatttat
     1981 taatattaaa aaaaccacgg tgcatgtcgt tgctattatt tgatataaat aaactcgacg
     2041 gtaggcatct tcaggctttt tagctcccac ccaatacccg acttttattt gtgttgctgt
     2101 accaattgat aagccaaaaa tacccacaaa acgtgcgata gtttgcgtat acaccatagc
     2161 agacattgcc caagtcccaa acgttgaaac cataaccatt atgacaattt gcgcaacatt
     2221 ataggaaaga ttttcgccgg ctgtcggcac cccaacactt aaaacaactc gatacataga
     2281 aggagataca tccctccatc ttttcaatgg aaagcgaaca tccttttttt gccgaatccg
     2341 tatagcgagg atgatactcg ctgcaagctg tgaaaaagca gaagatgcag cgacaccagc
     2401 tatacctaag acaggaagtc caaaaaaacc atatagggaa atagcatttc ccaaaatatt
     2461 gagaatattt gcgacaattg ctatatacat tgcatctttc gtatatccat acgcacgtaa
     2521 aacagtccct tgaagcatgt ttaatgccat aaaaaaagca ccaaagcctc caaaaataac
     2581 gagatattgc caggccatat ggcgcacatc atcttctatg gagtacaatg acaataatgg
     2641 agctgctcca aagaaaataa gaagcattat aaaaagtgcc acaagcgata tcatgaggac
     2701 gcttgcttgc gttacctgct ccgcctcttt gttgcgtttt gctcccaaat attgcgataa
     2761 aacaatgctc gttccaatac agataacatt aaaaagaatt tgtataaaaa aaatgtattg
     2821 tccgatcatg ccggcagcgg caacagcctt ttgagaccat gaactgagca taaaagtatc
     2881 aacggatgat aaaagaatac gaaagccatt ttcaaaaaga agaggtatag taagcgaaag
     2941 catcggcaaa cttgcacgat ttattttttc ttcaccattg ttaatttcca tagtaaacca
     3001 agcctactac ttttttattc ttttatccac tagccagcaa agaaaactat tcttaatccg
     3061 taaaaaagtt tactaatact tcgcagtatt caaaaaatgt aaaaagataa ccagagcata
     3121 aaaaaagccc ttcagaagag ctcaggtggt ctacggcaca aagatacaca atcgaccgct
     3181 gctttcttcc gaatctgacg gagttagaaa gatacctctt gcatagtacc tgagctcatc
     3241 aaaagagcct ttcaattata aataatgagc gtttccccag caaactctca aagacttaaa
     3301 aacacggaga gagggggatt cgaacccccg gtgccttttg ggcacacacg ctttccaagc
     3361 gtgcacctta aaccactcgg acatctctcc aaaaaatgaa gtattaatgc tttttcccgt
     3421 ggcatggatg ccacgtaaaa ccaacgcgac agtttgccag caaactgtct gttagatttg
     3481 cacatggatg tgcaaatcta accacggaga gagggggatt cggcttccac tcgaaaacgt
     3541 cctgttttct cctttcagcc cgcctacgct cctaaatcgc catccgtggc gatttgcgct
     3601 cccacttggt cgcgccgtcg cttcgtttcg aatccct
//
LOCUS       DAOSSW010000114         3335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_25747_length_3335_cov_6.940244, whole genome shotgun sequence.
ACCESSION   DAOSSW010000114 DAOSSW010000000
VERSION     DAOSSW010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25747_length_3335_cov_6.940244"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PKW26_07810"
     CDS             complement(<1..662)
                     /locus_tag="PKW26_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOQ93508.1"
                     /translation="MKIVLVGAGSAQFGSGMLGDIFASKELVGSHIVLHDINETALNK
                     MLSVANRFITEHKLNYTITASTNRKEAFQSADFIISSIEVGNRFALWEEDWKVPLQYG
                     MEQVYGENGGPGGVFHSLRIIPPILEIVKDAMDICPNAYIFNFSNPMTAICTTVKRAF
                     PEARFIGLCHEIGWLQSWLPRMLSLDLDDIYFKAAGLNHFSCMIEARHKKTGDDLYPQ
                     VR"
     gene            complement(962..3316)
                     /locus_tag="PKW26_07815"
     CDS             complement(962..3316)
                     /locus_tag="PKW26_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOQ93509.1"
                     /translation="MEFTQETIDALQVDEVAIMNRIAKELSIKLGQVSAVISLVKEGS
                     TIPFISRYRKEQHGSLDELQVRECDSFFKSYSNLEERRIEIIKGVFAQGKLTEHLHKA
                     IVEAKTLTELEDLWAPFKKKRKTRGMMAQEKGLEPLADLMLTLDTEAIEKEAEKFVVI
                     EAETDELKVPTVKDALDGAKDILAERTSQETENRSAVKAFYLRTGKIKVKGLGDEAKE
                     KTSVYKMYWEYEEPLNEIKAHRILAINRGEKEGELEVEYDLDVDGAIGVLKSRYVTYN
                     SYHDEAIEDSLVRLLSPAVLREIKSDLSEGADDHGIGIFSENLKNLLMQQPIKGTRVL
                     GIDPGIRTGTKCAALDETGKYLGDFLIHQVTAPEKAAFAIKKAIKEYNVQLVAVGNGT
                     GSREVQEIVSAVLSEHYPDVLYTVVDEDGASVYSASDIAREEFPDLDLTIRGAISIGR
                     RLQDPLAELVKIDPKSIGVGLYQHDLNQKKLSEMLDNVVSSVVNQVGVNLNTASASLL
                     KYVSGINNSLAKKIVAHRETTGKIVSREDLKKVSGMGEKTFEQCAGFLKIPESADPLD
                     NTWVHPENYAAAREVLPLVKKGSELDSPTKKELKEKFNLGETTIVDIVEELQKPNRDP
                     REGYPKPIMQKGVLSFEDLKEGMKVTGKIKNVVDFGAFVDLGIKETALLHISELSDSF
                     VEDPMSILKVGDIKECTIIGLDIDRKRISLSLKSDAGSRGSGQATSPRDANKTREGSS
                     AKKVVRVNRSDAPSKNKERQDNRQFAGNDEGTSYNPFAALLTKK"
BASE COUNT          900 a          754 c          603 g         1078 t
ORIGIN      
        1 tctcttactt gcggatataa atcgtcgccc gtttttttat gccgagcctc aatcatacaa
       61 ctaaagtgat ttaaacctgc tgccttaaaa taaatatcat cgagatctag agaaagcatg
      121 cggggaagcc aactttgcaa ccaaccaatt tcatgacaaa gaccaataaa acgagcctca
      181 ggaaaagcac gctttaccgt cgtacaaatt gcagtcatag ggtttgagaa attaaaaata
      241 taggcatttg gacaaatatc catcgcatct ttaacaattt caagaattgg aggaataata
      301 cgcaaggagt gaaatactcc gccaggacca ccattttcgc catacacttg ttccatgcca
      361 tattgaagcg gaaccttcca atcctcttcc caaagagcaa acctattacc cacttctata
      421 gatgaaataa taaaatctgc agactgaaaa gcttcttttc ggttagttga agctgttata
      481 gtgtaattaa gcttgtgttc agtaataaaa cgatttgcca ctgaaagcat tttgttaagc
      541 gctgtttcat ttatatcatg caatacaata tgtgaaccaa caagctcttt acttgcaaaa
      601 atatctccaa gcatcccaga accaaactgc gcgctccctg cgccaactaa aacgattttc
      661 attctttcct cctagactat aagtgtaggt taaaccttag ccattgtaaa gaacttttat
      721 ttatacttcc tgttaatcct gttttatcct gtcctcacaa aagcgactga aatactcatc
      781 ccgcaacatg gatgtcgcag gaaatacgct caacagtttt gcaagcaaaa ctgtcaaaat
      841 aattttttac acggatgtaa aaaattattt acctgtctac gaaatcagtc tctgcgagca
      901 aactctaaaa aaaaaactcc caagcaatcg ctatgggagc ttttcatatc aatgttttct
      961 cttacttctt tgttaagagt gcggcaaatg gattgtagct tgtgccttca tcattgcctg
     1021 caaattgtct attgtcttgt cgttctttgt ttttagaagg agcatctgag cgattcacgc
     1081 gtaccacttt ttttgcactg cttccttcgc gggttttatt tgcatcacgt ggggatgtcg
     1141 cctgcccact gcccctactt cctgcatcag actttagtga caatgaaata cgttttcggt
     1201 ctatatccag cccgataatc gtgcattcct taatgtcgcc aactttgagg atggacatag
     1261 ggtcttcgac aaaggagtcg ctcaattctg aaatatgcaa taaagcggtt tctttgatgc
     1321 ccaagtcaac aaaggcgcca aagtcaacga cattttttat tttcccggta accttcatgc
     1381 cttcttttag gtcctcaaag gagaggacgc ctttttgcat aataggttta ggatagcctt
     1441 ctcgcgggtc tcggtttggt ttttgtaact cttctacgat atcaacaatg gttgtttcgc
     1501 caagattaaa tttttcttta agttcttttt ttgtagggct atcgagttcg ctaccttttt
     1561 ttacaagggg aagcacctct cgtgcagcag cataattttc tggatgcacc caggtattat
     1621 cgaggggatc ggcgctttcg gggattttca aaaaaccagc acattgttca aaagtttttt
     1681 cacccatacc gctcactttt tttaagtctt ctcggcttac aatttttccc gttgtttcac
     1741 gatgtgcaac aatttttttt gcaagggaat tatttatacc agaaacatat tttaaaagag
     1801 atgccgatgc ggtgtttaag tttactccaa cttgattcac tactgaggag acaacattgt
     1861 cgagcatttc ggaaagtttt ttttggttta agtcatgctg atacaagccc accccaatag
     1921 attttgggtc tatttttaca agctcagcaa gcgggtcttg cagtcttctc ccaatcgata
     1981 tagcgccacg aatcgtcaag tcgaggtcgg gaaattcttc acgggcgata tcacttgcag
     2041 aatagacgga ggctccgtct tcatcaacaa cggtatacaa gacatcggga tagtgttcac
     2101 ttaaaacagc agaaacaatt tcttgcacct cccgggaacc cgtaccgttt ccaactgcaa
     2161 cgagttgtac attatattcc tttatcgctt tttttattgc aaaggcagct ttttctgggg
     2221 ctgtcacttg atgtattaaa aaatctccga gatattttcc tgtttcatca agggctgcgc
     2281 actttgttcc tgttcgaata ccagggtcaa tgccaaggac gcgagtacct ttaatcggct
     2341 gctgcataag aagatttttt aggttttcgc taaaaattcc aatgccatgg tcgtcagctc
     2401 cttcacttaa atcgcttttt atttctcgaa gcaccgcagg cgacaaaagc cgcacaagcg
     2461 aatcttctat tgcctcatcg tgatagctat tatatgttac atagcgagat ttcaaaacac
     2521 cgattgcgcc atcgacgtct aaatcatact caacctcaag ctctccttct ttttctccac
     2581 gattgatagc gagaattcta tgcgccttta tttcattgag cggctcttca tattcccagt
     2641 acattttata gacagaggtt ttttcctttg cctcatcgcc aaggcctttt acctttattt
     2701 ttcctgttcg taaataaaaa gcctttacag cgctacggtt ttctgtttct tgcgatgttc
     2761 tttctgcaag gatatctttt gcgccatcga gcgcatcttt tactgtcggt accttaagtt
     2821 catctgtttc tgcctcgatt acgacaaatt tttctgcttc tttttctatt gcttcagtgt
     2881 cgagagtgag catcaagtca gcaagcggtt ctagaccctt ctcttgcgcc atcatgccgc
     2941 gagtttttct ttttttctta aagggagccc aaagatcttc aagctctgtt aaggtttttg
     3001 cttcaacaat tgccttgtgc aaatgctctg tcaattttcc ttgagcaaaa acacccttga
     3061 taatttctat tcgtctttct tctaggtttg aataggattt aaagaaagaa tcgcattcgc
     3121 gaacctgcaa ttcgtcgagc gagccatgtt gctcttttcg atatcgggat ataaaaggga
     3181 tagtgctacc ctcttttaca agcgaaataa cagcgctcac ctgtccaagc ttaatagaaa
     3241 gctcttttgc aatacgattc ataatagcta cttcatcgac ctgaagtgca tcaattgttt
     3301 cttgtgtaaa ttccataaac tcatcttacg aaaaa
//
LOCUS       DAOSSW010000115         3297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_26138_length_3297_cov_4.710364, whole genome shotgun sequence.
ACCESSION   DAOSSW010000115 DAOSSW010000000
VERSION     DAOSSW010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26138_length_3297_cov_4.710364"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            583..1275
                     /locus_tag="PKW26_07820"
     CDS             583..1275
                     /locus_tag="PKW26_07820"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOQ93510.1"
                     /translation="MTEKLEKREYLFEIYKTYIAKDIKSFLREESILSFNKMLTWLAM
                     NNTSQLNKHSLSKIVGVSSRQIDRYIEVLEGTFVMASLPPFSKNKSKEITKMPKFYFY
                     DQGIINTIIQDFRPFHLRPDVGSQKEAFVFWELKKYADIRYAMYYWRTSDSQEVDFIL
                     VKDRLILPIEVKSQWDIKKIPPGLKAFFSFYPEVERAIVFYDGLEDQLLYENKTIFFA
                     PHYKASKALELL"
     gene            complement(1305..2666)
                     /locus_tag="PKW26_07825"
     CDS             complement(1305..2666)
                     /locus_tag="PKW26_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93511.1"
                     /translation="MQNLALCFPSALRRESLFSAISSLLSHEVNANREVSIYTDNPSA
                     TPPFVKEGIEGLKKKGVFCTLHYLEKRKETIENLATSFDKELLNYALLPPEDGIGYGV
                     NVNASMLGSAGSILISCDDDIISKPARIKPALLDALLEKMPSADKKEGDDDRLLLYFT
                     NRESLLDTVEEVDVNILQAYLDLFRQNEESNGKISENPILWINPGLYGDTGMGSARGT
                     LSLTGTSRAFLHQDYEGLKLSGEAINIHLQSTISAKTNLMAGQTAFYNKVPLAPFMPY
                     GRGTDGLSGLLSRLIYPGSRAAYSDFALYHASDGTRNNPAKTLTWVKPSISDLAMIVA
                     IVFRKETEEGFNYYGSLFSDIARLSNSSFVDHLHGAFIAQFNAVIEYYEKLLERYNRE
                     PASWAADMETHIENIQEKMRNPLSLFGKEGCDLSIERAKYHLEHYGEVLKIWPDLWKK
                     NLK"
     gene            complement(2685..>3297)
                     /locus_tag="PKW26_07830"
     CDS             complement(2685..>3297)
                     /locus_tag="PKW26_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93512.1"
                     /translation="FVWEIKMLSQKGLYTIEAYESGPELYASDGRQYKNTHPIPGIED
                     YTLVFYHPENKASTYYLASSSNTQFSINYGTKLEQKILVTRESGDFLGNRDKENFDEI
                     PPDDSTKTLSFTIYNPYPYALNFTTKFENGTHNTGTTVPPDDSWEKFTAPGFPTSLPP
                     GGRRDFDIIFTPTYNDEVFADFTIQAEGLGNFVLELSGSGSGF"
BASE COUNT          913 a          597 c          725 g         1062 t
ORIGIN      
        1 aaataagaga gttcctcgct ccatgagcga gttcattgtc ttggctaaat atcgccttaa
       61 atacgacatc tgactttggg tctaacaatt caatagtttc tttcattttc tcctcctttt
      121 tgcgacatgg acgccgcagg gtacgttttt tttaacatgg atgttagata aaacgtaccc
      181 tatatatatt agtagctttt tgaaggagtt ttttgtaatt tttaaaaaga tttctggaaa
      241 aaaataaaaa gtggggctaa tagtgaggac aggattaaca tgacacaaca tgattaacag
      301 gtacatgttt ttttgcatcc tacataattt tttacatccg tgtcgcggga aaagcgtttc
      361 cgtcgctttt gcgagagcaa tattcggata tatttaacgg tttcgttaaa attatctgga
      421 tatttttcac gtatcgggat atagtatagc tatgtatcag agcttaaaac gatagcacaa
      481 acagatggaa tagaatctgt ttctcccatc gatgagtggg aattttacac aaaccggttt
      541 caaaactttt tgcattttgg tgctttgcct ggactctacg gtatgacaga aaagcttgaa
      601 aaacgtgaat atttatttga aatttataaa acctatattg caaaagatat taaatctttt
      661 ttaagagaag aatctatatt gtcttttaat aaaatgctca cctggcttgc tatgaataat
      721 acgtcacaat taaataaaca cagtctttca aaaattgtcg gtgtcagttc aaggcaaatt
      781 gatagatata ttgaagtgct cgaaggcacc ttcgttatgg cgagtctccc gcctttttca
      841 aaaaacaaat ccaaagaaat aactaaaatg cccaagtttt atttttatga ccaaggtatt
      901 atcaatacga ttatacagga ttttagaccc tttcacttgc gacccgatgt cggtagtcaa
      961 aaagaagcct ttgtgttttg ggaattaaaa aaatatgcag atatacgcta tgcaatgtat
     1021 tattggcgga cgagcgatag tcaagaggtc gattttattt tggttaaaga taggcttatt
     1081 ttacccatcg aggttaaaag ccagtgggat ataaaaaaaa taccgccggg actaaaggca
     1141 tttttttcat tttatcctga agtggaaagg gcaattgttt tttacgatgg cttagaagat
     1201 caactgctct atgaaaataa aactatattt tttgcaccgc attacaaagc ctcaaaggcc
     1261 ttagaattgt tgtaaaacta aaaattcttg cgtctttgcg agagctattt taagttcttt
     1321 ttccacaaat cgggccagat tttaaggact tcgccgtagt gttcgaggtg gtattttgct
     1381 ctttctatag aaaggtcgca gccttctttg ccaaagaggg atagggggtt gcgcattttt
     1441 tcttggatgt tttctatatg tgtttccatg tccgctgccc aggaggctgg ttcccgattg
     1501 tagcgctcta gtagtttttc atagtattcg attacagcat taaactgtgc tataaaggcg
     1561 ccgtgtaggt ggtctacaaa gctagagttt gaaaggcggg cgatgtctga aaagaggctg
     1621 ccgtagtagt taaagccttc ttctgtttct ttacgaaaga cgatggcgac tatcatggcg
     1681 aggtcagaaa tagagggttt tacccaggtg agtgtttttg ctgggttatt tcttgtgccg
     1741 tcggaagcgt ggtaaagggc aaagtcgctg taggcagccc tgcttccggg ataaataagg
     1801 cggcttaaaa gcccggataa cccgtcggtg cctcttccat agggcataaa tggggcaagg
     1861 ggtactttgt tgtaaaaggc tgtttgccct gccatgaggt ttgtttttgc agaaatagtg
     1921 ctttgaaggt gtatgtttat agcttcgcca gatagtttta agccttcgta gtcttggtgc
     1981 aaaaacgctc tcgacgttcc agtaagcgac agagtgcccc gggcagagcc catgccggtg
     2041 tcgccgtaga gccctgggtt tatccaaagt attgggtttt ctgatatctt gccgttgctt
     2101 tcttcgtttt gcctaaaaag gtcgaggtag gcttgtagta tatttacatc tacttcttcg
     2161 acagtgtcca aaagactttc tcggtttgta aaatacaata aaagccgatc atcatcgccc
     2221 tcttttttgt cagcagaagg cattttttct aaaagcgcat cgaggagagc cggttttatt
     2281 ctagctggtt tagaaataat atcatcgtcg caggaaatga gtatgcttcc tgctgagcct
     2341 agcatagaag catttacatt taccccatag cctatgccgt cttctggtgg caggagggca
     2401 tagttcaaaa gctctttgtc aaagctcgtt gccaagttct ctatggtctc ttttcttttt
     2461 tctagataat gcaaagtgca aaaaacgcct ttttttttaa gcccttctat gccttctttc
     2521 acaaaggggg gagtcgcaga aggattgtcg gtatatatac tcacttctct gtttgcattt
     2581 acctcatgcg agagaagaga ggaaatagca gaaaataagc tctccctccg cagggcagag
     2641 ggaaagcata aggctagatt ttgcatgagc cttcttggat ttaattaaaa accgcttccc
     2701 gatccagaga gttctaagac aaagtttccc aatccttctg cttgtatggt aaaatctgca
     2761 aaaacttcat cattgtaagt tggcgtaaaa ataatgtcaa agtctctccg tccgcctggg
     2821 ggaagagagg tggggaaacc tggagctgta aatttttccc aactgtcatc tggcggtaca
     2881 gttgttcctg tattgtgagt accgttttca aacttggttg taaaatttaa agcataggga
     2941 taaggattgt agatagtaaa ggaaagcgtt tttgtggaat catctggagg aatttcgtca
     3001 aagttttctt tatctcggtt ccctaaaaaa tcaccacttt ctcgtgttac aagtattttt
     3061 tgctcgagtt ttgtgccata gtttatggaa aactgagtat tagaactact tgcaaggtag
     3121 taagtgcttg ctttattttc tggatggtaa aaaacaaggg tgtagtcctc tatgccagga
     3181 attggatggg tgtttttata ctgtctgcca tcggaagcat agagttcggg accgctttcg
     3241 tatgcctcta tcgtatagag ccctttttgg ctaagcattt ttatttccca aacaaag
//
LOCUS       DAOSSW010000116         3242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_26745_length_3242_cov_6.450894, whole genome shotgun sequence.
ACCESSION   DAOSSW010000116 DAOSSW010000000
VERSION     DAOSSW010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26745_length_3242_cov_6.450894"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PKW26_07835"
     CDS             <1..240
                     /locus_tag="PKW26_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ93513.1"
                     /translation="LLLDEPSMGLAPILVEEIFSIIETINAQGTTVLLVEQNAYKALA
                     IANYAYILETGVIIKEGEAKTLLKDAEVKAAYLGT"
     gene            285..932
                     /locus_tag="PKW26_07840"
     CDS             285..932
                     /locus_tag="PKW26_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HOQ93514.1"
                     /translation="MIVAKVMTKKPRYVRPQTSVNDVRELMAKEKIGKLPVLDDNDRL
                     VGIITKKDLMQSSPSSGTTLDMYEISYLLAKLKAEKIMTKPVITVQETEVVEEAAKIM
                     ADKEIGCLPVLKGSLLVGIITESDLFRCFVEMFGARYGGVRVIFCLDEKPGQLAKFTE
                     KVANASGNIVSIVTGDCEDSTRRQCTVKVSGISEEKMVEILKELDAEIEDIRLIQ"
     gene            complement(1012..1506)
                     /locus_tag="PKW26_07845"
     CDS             complement(1012..1506)
                     /locus_tag="PKW26_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOQ93515.1"
                     /translation="MNTVSLFTPRFTTNFFDAFDREFLNYASKEEKIYKPLVDVRELE
                     NAYILEMELPGLDENNIDISLKDNVLSIASISEAVKEESHEEGKKEVKEPKKDLVKAR
                     DEQKDEAKWLIRERKTYSFTRRFTLPKDIDENAVSAQFKNGLLTITLNRKEEAQAKTI
                     KISA"
     gene            1890..2357
                     /locus_tag="PKW26_07850"
     CDS             1890..2357
                     /locus_tag="PKW26_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOQ93516.1"
                     /translation="MHEYVNLSELIHRGGLYYDVKGATFQEVYKNLSQLVSLPRELNP
                     EFFYTELCLREEMMSTAVGKGIAIPHPRHPILTQFDEQRIIVCFLEESLGIETPDLKP
                     VFALFVVLSSNKSTHLNVLSNLAFLFQKQTFIDVLKTRPQEHELLTEIKKYQM"
     gene            2367..>3242
                     /locus_tag="PKW26_07855"
     CDS             2367..>3242
                     /locus_tag="PKW26_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOQ93517.1"
                     /translation="MNKKQLDAVSKSIRSLSMDAIQKAQSGHPGLPLGCAELASLLYG
                     EIMQHNPANPLWINRDRFVLSAGHGSMLLYAILHLSGYNLSLDDIKNFRQLGSKTPGH
                     PEWGYTEGVDVTTGPLGQGLAMAVGMAIAETMLASKFNTEKTKLIDHYTYVLLGEGCL
                     MEGVTSEASSLAGHLGLGKLIAFYDENRISIDGSTDLTFTEDIQKRYEAYGWQVLSGS
                     MYDYEEIAALVAEAKKDSRPSFIMLKSTIGKHAPTVEGLAAAHGAPLGKEGIQKAKEV
                     LGIHPDESFYVDPEAY"
BASE COUNT          979 a          535 c          675 g         1053 t
ORIGIN      
        1 ttgcttttgg atgagccctc tatgggtctt gctcctatac ttgttgaaga gattttttcc
       61 atcattgaga ctatcaatgc acaaggaaca actgttcttt tggttgaaca aaatgcctat
      121 aaggcgctcg caattgcaaa ttatgcttat atactagaaa ctggtgttat aataaaagaa
      181 ggtgaagcaa agactttgtt gaaggatgct gaagtaaagg ctgcatatct gggaacctag
      241 gattaggttt gtgtatttac atctataaaa aggggttgta aaagatgatc gttgcaaaag
      301 taatgactaa aaaaccgcgc tatgttcgcc cacagacgtc ggtaaatgat gtgagagaac
      361 ttatggcaaa agagaagatt ggaaaactgc cagtactcga tgataacgat agacttgtgg
      421 gaataattac aaaaaaagat ctcatgcagt cgagcccttc ttcggggaca accttggata
      481 tgtatgaaat aagttatttg cttgcaaaat taaaggctga aaaaattatg acaaagcctg
      541 ttataacggt gcaagaaaca gaggttgtcg aagaagctgc aaaaataatg gcagataaag
      601 agataggctg tttgccggtt ctaaaaggct ctttgctcgt tggtattatc acagagagtg
      661 atttatttcg ttgctttgta gaaatgtttg gtgcacgtta tggtggtgtt cgtgttattt
      721 tttgcttaga tgaaaaacct ggacaacttg ccaaatttac cgaaaaagtt gcaaatgcct
      781 cgggtaatat tgtttctatt gtaacgggtg attgtgagga cagcactcgg cgccaatgta
      841 ctgtaaaagt ttcgggaatt tcagaagaga aaatggtgga aatcctaaaa gaactcgatg
      901 ctgaaattga agatattcgt cttatccaat aaataaaaaa aaacactcct cgcctacttg
      961 cgggagtgtt ttttttaggt ctgtaaaaca gactgattta aatcgatgaa actaggcaga
     1021 aattttaatt gtttttgcct gagcctcttc cttccgattg agcgtgatag tcaaaagacc
     1081 gtttttaaat tgtgcactta ctgcattctc gtcgatatct tttggaaggg tgaatcttcg
     1141 agtaaagcta taggtcttac gttctcttat aagccatttt gcttcgtctt tttgttcatc
     1201 acgggctttt accaggtctt tttttggctc ttttacttct tttttgcctt cctcatggga
     1261 ctcttctttt acagcttcac taatacttgc aatactgagg acattgtcct tgagactgat
     1321 atcaatattg ttttcatcaa gtccaggtaa ttccatttca agaatatagg cattttctag
     1381 ttctctcacg tctacaagcg gtttataaat tttttcttct ttagaagcat agtttaagaa
     1441 ttccctgtcg aatgcatcga aaaaatttgt ggtgaatcgt ggtgtaaata atgatactgt
     1501 gttcataata acctccaaag ttttatattg ttattttcat aactcgtatg aaatcgcttt
     1561 tcacttatta tatatgcaat tttcgtgcca actttcgagt tttggcaagc acaatgcgtc
     1621 attttttatc taaaaaataa agaaaaaagg tgaaatttct tagttttttt tattttttca
     1681 tgaagagaga aatttagatg ttaaaacata gtgtcatatt gatacatttt tgtgaagttt
     1741 tataaagtct tgaataaaaa taaaacaatt tgatacgctt attttacatc tgtgttaatt
     1801 tgacacggtt ctccttgttt tttttctgtt gaattcagct taaagaagtg ttattatgag
     1861 ggtatctttt gaaaataact ggggtatata tgcatgagta tgttaattta agtgagctca
     1921 ttcatcgggg cggactttat tacgacgtaa agggtgcaac atttcaagaa gtatataaaa
     1981 atctctcgca gctcgtttct ttacctaggg agttgaaccc agagtttttt tacacggaac
     2041 tttgtcttcg agaagaaatg atgagcacgg ctgtcggcaa aggaattgca attccgcacc
     2101 caaggcatcc tattttgacg cagtttgatg agcaacgaat tattgtttgt tttttggaag
     2161 aatcgcttgg tatagaaaca ccggatttaa agcctgtttt tgctttgttt gtggtattgt
     2221 caagtaataa atcaacgcac ttaaatgtac tttcaaattt agccttttta tttcaaaagc
     2281 aaacttttat cgatgtatta aaaactcgtc ctcaagaaca tgaattatta actgaaataa
     2341 aaaaatatca aatgtaagga aattaaatga acaaaaaaca acttgacgct gtttctaaat
     2401 caattagaag tttaagtatg gatgcaattc aaaaagcaca atctgggcac cctggtttac
     2461 cgcttggttg tgcagaactc gcaagtcttt tatatggtga gataatgcag cataatcctg
     2521 caaacccact ttggataaat cgtgatcgtt ttgtgctttc agctggacac ggttctatgc
     2581 ttttatatgc gatactgcat ttgagtggtt ataatctcag ccttgatgat ataaaaaact
     2641 ttcgtcaact tggttcaaaa acgccggggc atccagagtg gggctatact gaaggtgttg
     2701 atgtaacaac tggccctctt gggcaaggtt tggcgatggc ggttggtatg gcgattgccg
     2761 aaacaatgct tgcttcaaaa ttcaatacag aaaaaacaaa acttatagac cattatacct
     2821 atgtgctttt gggtgagggc tgtctcatgg aaggcgtgac ttctgaggca tcaagtttgg
     2881 cagggcattt agggcttggt aaactcattg ccttttatga cgaaaaccgc atctcaattg
     2941 acggttctac ggacctaacc tttacggaag atatacaaaa gcgatatgaa gcttatggct
     3001 ggcaggtgct ttctggttcc atgtatgatt atgaagagat agccgccttg gttgcagaag
     3061 caaaaaaaga cagccgtcct tcttttatca tgcttaaatc aacgataggg aaacatgctc
     3121 cgacggtaga aggcttggct gcagcacacg gtgcgccgct tggaaaagaa ggcattcaaa
     3181 aagcaaagga agttctaggg attcatcctg atgaaagctt ttatgttgat ccagaagcct
     3241 at
//
LOCUS       DAOSSW010000117         3166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_27627_length_3166_cov_6.006107, whole genome shotgun sequence.
ACCESSION   DAOSSW010000117 DAOSSW010000000
VERSION     DAOSSW010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27627_length_3166_cov_6.006107"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PKW26_07860"
     CDS             <1..568
                     /locus_tag="PKW26_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOQ93518.1"
                     /translation="WIAYSFAILTVALFAYYPASKVLKEYQIQRLIVFIDPTTDPRGS
                     GWHIIQSKIAIGSGNVFGQGFLKGTQSHYRFLPQQSTDFIFSILSEELGFIGGIFVFA
                     LYLFIFIRIYYILKETNNTYGYYVAIGIVAMFFFHFVVNVGMVMGIMPITGIPLLFIS
                     YGGSSLWNAMICVGLLMSINARRLDFSA"
     gene            593..3160
                     /locus_tag="PKW26_07865"
     CDS             593..3160
                     /locus_tag="PKW26_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOQ93519.1"
                     /translation="MIKKTLIQPLDLFSKSLLSIQKPARYLSGDFGQIKKPFTENDSL
                     FNFCIAFPDLYEIGMSNQAIRIIYNELNKYNSIRAERVFAPELDFEKLLRETKTPLFT
                     IESGIPLNKLDMLGFSVGYELNATSLLSILDSGKIPLLNAERGEDDPIIIAGGCGITN
                     PRPFQNSIDAFFIGEAENELFLLIEALSLLKKEGASRVDLLARFTEHPAVWVPGKKAT
                     KATYHDFGKKPAPSSFFPTANIKAVQEHGVVEVMRGCPNGCRFCHAGVFYRPQRMKSI
                     PDIVAEVDKLVFEGGFREISLTSLSSGDYTGIDMLLDILTKRYEHLPVSFQLPSLKVN
                     SFTLPLLEKISEVRKSGLTFAVETPEEAWQLSLNKEVFEERLYDIIMNAKKRGWSKAK
                     FYFMIGLPLPEAEISEEEAIVAFMLKIQQATRIQCTVNVGTFIPKPHTPYQWARQISM
                     EESKRKLDFIRFSLPKGKFRVSTHNEFVSFIEGMISRGDERVGDALLSAYKKGCRLDA
                     WDDHFNKTAWKEVFDELDWNVYEEVSRSYSLEEKLPWDDISLGVSKGFLRREAEKSMR
                     GELTAACALDCASPCGICTPNHGLLTQKVSEDVYKSIPNSQFLEPFSIKREESNVPVL
                     YRLLLQYTKVDSAAFYPHLSIQDLFHRAFLRSALPFVYTAGFNPIPRLELASSTSIGI
                     TSEAEIASCLLYEPVDPDYLKQLLNKNLPDGLQICNLSLFPVTNRIKRESLASHYWGA
                     SYEYSFYTDIFSKAEIEEGLSDERIQNIFSETEHFFNTKPVEEKDGKESFVLSLRLPF
                     KLDRPFRDALEEVFAKKLYEIVHIYKTQTFSNYKGEVQSYFESYAEIAKQNAAFLDE"
BASE COUNT          926 a          583 c          637 g         1020 t
ORIGIN      
        1 ctggatagcc tattcttttg ccattttaac tgttgcgctt tttgcctatt atccagcaag
       61 taaagtttta aaagaatatc aaattcaacg cctcattgtt tttattgacc caacaactga
      121 ccctcgaggt tctggttggc atataattca atcaaaaatt gcaattggat ctgggaatgt
      181 ctttggacag ggttttttaa aaggaacaca aagtcactat cgatttttgc cacaacaaag
      241 tactgacttt atctttagta tattgtctga agagttgggc tttatcggtg gcatctttgt
      301 ttttgcactc tatttattta tctttattcg tatttattat attttaaaag aaacaaataa
      361 tacctacggt tactatgtcg ccattgggat tgttgctatg tttttctttc actttgtggt
      421 aaatgtagga atggttatgg gaataatgcc cataacgggt attccgcttt tatttatttc
      481 ttacggcggt tcatctttat ggaatgcaat gatttgcgtg ggtcttttga tgagtattaa
      541 cgctcgtagg ttggattttt cagcctagga ctttctcttt tttaaggaaa caatgataaa
      601 aaaaacactc atacagcctc ttgatttatt ttcaaaatct cttttgtcta tacaaaaacc
      661 tgctcgttat ttgagcggcg atttcgggca gataaaaaag cccttcacag agaatgattc
      721 gcttttcaat ttttgtattg ccttcccgga cctatacgaa atcggaatga gtaatcaagc
      781 aatacgcatt atttataatg agctaaacaa atacaatagc attcgtgcag aacgtgtttt
      841 tgcccctgag cttgattttg aaaaactttt aagggaaact aaaactccct tgtttacaat
      901 tgaatcgggc attccgttga ataagcttga tatgcttggt ttttctgtcg gatacgaact
      961 gaatgcaaca agtttactgt ccattttaga ttctgggaag ataccgcttt taaatgctga
     1021 aagaggggaa gatgacccca ttatcatagc tggtggttgt ggcataacaa atccccgacc
     1081 tttccaaaat tctatagatg catttttcat aggtgaggct gaaaatgaat tgttcttact
     1141 catagaagcg ctttcacttt taaaaaaaga gggcgcgagt agggtagact tactcgcacg
     1201 cttcacggaa catcctgcag tttgggtgcc tggaaaaaaa gcgacaaaag cgacctatca
     1261 tgattttgga aaaaaacctg cgccatcatc tttttttcct accgcaaata ttaaggctgt
     1321 acaagaacat ggcgttgttg aagttatgcg aggctgtcca aatggttgcc gattttgtca
     1381 tgcgggtgtt ttttatagac cacagcgcat gaaatccatt ccagatatcg tagctgaagt
     1441 tgataagctt gtttttgaag gtggctttcg agagataagt ttaacctcgc tttcatctgg
     1501 tgattatact ggtatagata tgctcttgga tattttgaca aaacgctatg agcatttgcc
     1561 tgtttctttt caacttccgt cgctcaaggt aaattctttt accttaccct tgcttgaaaa
     1621 aatttcagag gtgcgaaaat ctggcttaac ctttgcggtg gaaactcccg aagaagcttg
     1681 gcaactttca ctcaataaag aagtttttga agaacgtctg tatgatatta taatgaatgc
     1741 aaaaaagaga ggttggtcaa aggcaaagtt ttattttatg attggattgc cacttccaga
     1801 agctgaaata agtgaagaag aagcaattgt tgcgtttatg ttaaaaattc aacaagcaac
     1861 ccgtattcaa tgcacggtaa atgttggtac ctttatccca aaaccacata ctccctatca
     1921 atgggcgcgg caaataagca tggaagagtc gaagagaaaa ctagatttta ttcgcttttc
     1981 gcttcctaaa ggaaaatttc gagtaagcac gcacaatgaa tttgtctcct ttatagaagg
     2041 tatgatttct cgtggcgatg agcgtgtagg ggatgcactt ctttcggcat ataaaaaagg
     2101 ctgccgcctc gatgcttggg acgatcactt taataaaact gcatggaaag aagttttcga
     2161 tgaactcgac tggaatgtct acgaagaggt ttctcgaagc tatagccttg aagaaaaact
     2221 tccttgggat gatataagcc tcggagtaag caagggcttt ttacgacgag aggcagaaaa
     2281 atcgatgcgt ggtgagctaa cagctgcctg tgccttagat tgtgcttctc cttgtggtat
     2341 ttgtacccca aatcatggct tgttaaccca gaaagtttcc gaagatgtat acaaaagtat
     2401 accaaactcg caatttttgg agccatttag cataaaacgc gaagaaagta atgttcctgt
     2461 cctgtatagg cttcttttac agtatacaaa ggtagattct gctgcctttt atccgcattt
     2521 gtcgatacaa gacctttttc atcgggcttt tttacgtagc gccttgccat ttgtctatac
     2581 agcaggcttt aatcccatac cgcgcctaga attggcaagt tctacttcaa taggaattac
     2641 ttctgaagct gaaattgcct cttgcttgct ctatgagcct gttgatccag attatttaaa
     2701 gcagctttta aataaaaacc ttccagatgg cttacaaatc tgcaatctct ctctttttcc
     2761 ggttacaaac cgcattaaac gagaatcttt ggcatcccat tattgggggg catcctatga
     2821 atacagcttt tatacggaca ttttttcaaa ggcagagata gaagagggct tgtctgatga
     2881 gcgcatacaa aatatttttt cagaaacaga gcattttttt aatacaaagc ctgtagaaga
     2941 aaaagatggc aaagaatctt ttgtcttgtc tttgcgtttg ccattcaagt tggacagacc
     3001 ttttcgagat gcccttgaag aagtttttgc aaaaaaatta tatgaaattg tacatatata
     3061 caaaacgcag actttttcca actacaaggg agaagtccag tcttattttg aaagctatgc
     3121 ggaaatagca aaacagaacg ccgctttttt agatgagtga tgctag
//
LOCUS       DAOSSW010000118         3133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_28014_length_3133_cov_5.961014, whole genome shotgun sequence.
ACCESSION   DAOSSW010000118 DAOSSW010000000
VERSION     DAOSSW010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28014_length_3133_cov_5.961014"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PKW26_07870"
     CDS             complement(<1..343)
                     /locus_tag="PKW26_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93520.1"
                     /translation="MKWVQFFCLFLLFSLLPFYIRAEHLIPLIGLPSEDAKSLYSYSS
                     DAAEIEFFADGFWNMGLKQNIFLPFDIKQALKLDMPVFSQEIELSVWFLLNKSWYFEA
                     AFAEGFEKNSIA"
     gene            complement(340..1524)
                     /gene="dinB"
                     /locus_tag="PKW26_07875"
     CDS             complement(340..1524)
                     /gene="dinB"
                     /locus_tag="PKW26_07875"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOQ93521.1"
                     /translation="MITNKDQYIKTFLHIDLDAFYASVEQLDFPEYRGKPVIVGSLPA
                     DKRGVVSTCSYEARRYGVHSAMPIYKAYQLCPQAIFLRGRMHRYQEKSKEVMSLFQNF
                     TPSVQQISIDEAFLDISGSEKLFGKPAALAQKIKNTVFEKTGLSVSVGIAHTKYLAKI
                     ASGMSKPDGLYEIAYGKEEAFMLELPLKKVWGIGEKSLQRLQEAGFSTTSQVHSASMQ
                     LLQSLFGKSQGEFLWKVVRGIEAERFDERVAHKSLSEEQTFEIDIRDKNTIEKILLDL
                     SQNIFFRLLHSNLQSKTVQLKIRYSDFTTVSIQETHERIIATSDALFEIAKRLFYKKY
                     EKDRYLRLLGLGFANVKEGKEEAQQELFAFENEKKKKLEKAIVDLQKKDPSVIIKKAR
                     LL"
     gene            complement(1511..>3133)
                     /locus_tag="PKW26_07880"
     CDS             complement(1511..>3133)
                     /locus_tag="PKW26_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93522.1"
                     /translation="LLSANDEKLHPSFEASLQGIQTYIENNVEYENLHAILIDFQEKK
                     LKKGLRIDIGTKNHLAPLPLVRSFIHSLEKNTIPYTIEGGFSSLVRLGFTSENPVLAF
                     LQNKEIPALALNTNENSIAEAVFLLTEHIKNYNPNSLVEESSQYSFFYIFEHFFSISE
                     IHLVALIIILAFLTLLIIVFFPFLLGKNRFLHKEEFSKSWPLLPLLILLNAFFFYISQ
                     PFMSLLFPHFESLPQFAFLIKIIISLLFLFFISFAQIIFKFPLSTFIYGYLGSFIAFI
                     NIFVFTFIDISLFIPFSFEFLIILITSSFKKRKNLFLTIALMVLPFLPYLLFTDGAIL
                     NNIFRIASRTSFIGNVLLALLLIPFQIVWIKILIRMKHFGKQKGVTFKRILIDVLMNL
                     GAIFSIVLFIQILIHTDTIPRTKLEKPKEISTTDKSFIEHVSTHAINYDTEDIECIIE
                     SSLPVLRYEIKIHSASLLPIYEANYPYDILTEQEWALFHLDDYPPNPLLLRFSSDASL
                     DKRLYITAYLLDGNEVVKTEEDFFYRGIDDNK"
BASE COUNT         1056 a          495 c          579 g         1003 t
ORIGIN      
        1 ctgcaatgct gtttttttca aagccctctg caaaggctgc ttcaaaatac caggatttat
       61 ttaataaaaa ccaaacagag agttctattt cttgagaaaa aacaggcatg tcaagtttga
      121 gtgcttgttt gatatcaaat ggtaaaaaaa tattttgctt gagccccatg ttccaaaaac
      181 catctgcaaa aaattcgatt tcagctgcat cggaagaata tgaataaagt gatttagcat
      241 cttcacttgg aagtccaatt aaaggaatga gatgttcagc tcgtatataa aaagggagga
      301 gagaaaataa aagaaaaaga caaaaaaact gtacccattt cataaaagcc ttgccttttt
      361 tataataact gaggggtctt ttttttgtaa gtcgacgatt gctttttcaa gctttttttt
      421 cttttcattt tcaaaggcaa atagttcttg ttgcgcttcc tccttgcctt cttttacatt
      481 tgcaaagccg agccccaaaa gtcgaagata gcgatctttt tcatacttct tataaaataa
      541 tctttttgca atttcaaata gggcatctga agttgctatg attctttcat gtgtttcttg
      601 aatagaaaca gttgtaaagt cactgtaacg aattttaagc tgtactgtct tgctttgtag
      661 gtttgaatgc agtaaacgaa aaaaaatatt ttggcttaaa tctaaaagaa ttttttcaat
      721 ggtgttttta tctcgtatat ctatttcaaa ggtttgctct tcactcagag atttatgtgc
      781 gactcgttcg tcaaaacgct cggcttctat tcctcgaaca accttccata aaaattcgcc
      841 ttgacttttc ccaaacaagg attgcaacaa ttgcatactt gcactatgta cttgtgaagt
      901 tgtagaaaac cctgcctctt gtaaacgttg cagagatttt tctcctatac cccagacttt
      961 ttttaagggc agctccagca taaaagcttc ttcttttccg taggctattt cgtataagcc
     1021 atcgggcttt gacattccag aagcaatctt tgcaagatat tttgtatgcg ctatcccgac
     1081 ggaaacagat aagcctgttt tttcaaacac ggtatttttt attttttgtg caagagcagc
     1141 aggcttgcca aataattttt cactaccgct tatatcgaga aaggcctcat cgatagaaat
     1201 ctgttgtacg cttggtgtga agttttgaaa aagactcatc acttcttttg acttttcttg
     1261 atatcgatgc atccttccgc gcagaaaaat agcttgggga caaagttgat aggctttata
     1321 aataggcata gctgaatgaa ctccgtagcg tcttgcctca taagaacagg tagaaacaac
     1381 tccacgttta tctgcgggta aacttcccac aattacaggc tttccgcgat actctggaaa
     1441 atctaattgt tcaaccgatg cataaaaagc atcgaggtca atatgtaaaa aggtttttat
     1501 atattgatcc ttatttgtta tcatctatgc ctcgataaaa aaaatcctct tcagttttta
     1561 caacttcgtt tccatcgaga aggtaagcgg tgatgtagag ccttttatcg agcgaggcgt
     1621 ctgaagaaaa acgaagcaaa agggggtttg gtggataatc atctaaatga aaaagtgccc
     1681 attcttgttc cgttaaaata tcgtatggat aatttgcctc ataaataggt aacaaggagg
     1741 cagagtgaat tttaatttca taacgtaaaa cagggagact cgattctatt atacattcaa
     1801 tatcttccgt gtcataattg attgcatgag tagatacatg ctcgataaaa gatttatccg
     1861 tcgtagaaat ttcttttggc ttctcaagtt ttgttctcgg tatagtatca gtgtgaatta
     1921 aaatttggat aaagagaaca atggaaaaaa tagcaccgag gttcatcagt acgtcaatta
     1981 aaattcgctt gaaggtaaca cccttttgct ttccaaagtg cttcattcta ataagtattt
     2041 ttatccatac tatttgaaag ggaattaata ataaagcgag caaaacgttt ccaataaagc
     2101 ttgttcgact tgctatacga aatatattat ttagtattgc gccgtcggta aaaagcagat
     2161 agggaagaaa gggtagaacc atgagtgcaa ttgttaaaaa aagatttttt ctttttttaa
     2221 aagaacttgt aatgagtata ataagaaact caaaactaaa gggtataaag agtgaaatat
     2281 cgataaaggt aaaaacaaaa atatttataa aggcaataaa tgagccaagg tatccataaa
     2341 taaatgttga gagaggaaat ttaaatataa tttgtgcaaa agaaataaaa aataaaaaga
     2401 gcaagctaat aatgattttt attaagaagg cgaattgagg aagtgattca aaatgtggaa
     2461 ataataagct cataaaaggt tgtgagatat aaaagaaaaa agcattgagg aggattaaga
     2521 gtggcaaaag tggccaggat tttgaaaact cttccttatg taaaaaacga ttttttccga
     2581 gcaaaaaggg aaaaaataca atgatgagca gtgttaaaaa cgcaagaata ataatgagtg
     2641 caacaagatg tatttctgag attgaaaaaa aatgttcaaa gatataaaaa aaactgtact
     2701 gactactttc ttctacaaga ctgtttggat tataattttt tatatgttca gttaaaagaa
     2761 aaacagcttc tgcgatactg ttttcgttag tgtttagtgc aagtgctgga atttctttgt
     2821 tttgtagaaa agctagaaca gggttttcac tcgtaaaacc taatcgaaca agtgaagaaa
     2881 aaccaccttc aatcgtgtag ggaattgtgt ttttctcgag agaatgtata aaggaacgca
     2941 caagaggaag aggtgcgagg tgattttttg taccaatatc tatacgcaag ccctttttga
     3001 gttttttttc ctgaaaatct atcagaatag cgtgaagatt ttcgtattct acattatttt
     3061 ctatatatgt ttgtattcct tgcaatgaag cttcaaaaga tgggtgcagt ttttcatcat
     3121 ttgcgcttaa aag
//
LOCUS       DAOSSW010000119         3051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_29067_length_3051_cov_6.306409, whole genome shotgun sequence.
ACCESSION   DAOSSW010000119 DAOSSW010000000
VERSION     DAOSSW010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29067_length_3051_cov_6.306409"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PKW26_07885"
     CDS             <1..222
                     /locus_tag="PKW26_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93523.1"
                     /translation="FICAPQSIEVNENGVLESLSRLSVSHAVKQKLFLNASYSYASGL
                     ENFVPNSESPFPLFSASRHSVSCSISAFF"
     gene            complement(224..598)
                     /locus_tag="PKW26_07890"
     CDS             complement(224..598)
                     /locus_tag="PKW26_07890"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOQ93524.1"
                     /translation="MQISKTFIEVIKHEGVAAIVTVAQNEAHIANTWNTYIRIVDNKS
                     IIIPVGGMGITQENLEINPKVKMTVGTREVQGLRYMGTGFLVEGRGQVDESGANYDFI
                     KKEFPWARAALVISPENITQTL"
     gene            788..2077
                     /locus_tag="PKW26_07895"
     CDS             788..2077
                     /locus_tag="PKW26_07895"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOQ93525.1"
                     /translation="MKYDLIIVGGGAAGLYAGAQLASYCDRKKKENKTEAKNIAIIDS
                     SPELGKKLLLTGAGQCNLTQAGSIKDFISHYGENGSKIRSLLYAHNNSDTMQFFESLG
                     LRLFEREDGKVFPQSLSAKEVRNLLLARCKKNIEIQTNTKLIDIKILAENTGFKLILE
                     KENKEIVLQTRFLLLSCGGASYPKTGSDGSIFPILKKMGLSVVKLKPALVPFFVDNYP
                     FKELAGIAIESVHIKKVGEKMAKKSFEPLLLTHDSFSGPTALHFSRYLDQGDKIFFDY
                     LPACKEEELVAEVQTKALRNNKQVASFFSQLPVLKEAGIPKRFVEVMCQRSSIDPTTK
                     ISAVSGKKLRALIHLFKSDVFRVSGTKAWQLAMITAGGVSLEELDLKSLSSKKYPSLF
                     FAGEILDIDGDSGGYNLQFAFSSAKKATESIIASWEK"
     gene            2171..2255
                     /locus_tag="PKW26_07900"
     tRNA            2171..2255
                     /locus_tag="PKW26_07900"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2205..2207,aa:Ser,seq:tga)
     gene            2492..2579
                     /locus_tag="PKW26_07905"
     tRNA            2492..2579
                     /locus_tag="PKW26_07905"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2526..2528,aa:Ser,seq:gct)
     gene            2978..3051
                     /locus_tag="PKW26_07910"
     tRNA            2978..3051
                     /locus_tag="PKW26_07910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3012..3014,aa:Arg,seq:gcg)
BASE COUNT          901 a          599 c          640 g          911 t
ORIGIN      
        1 tttatctgtg ctcctcagtc tatcgaagta aatgaaaacg gtgtacttga aagccttagc
       61 cgactttctg tttctcatgc agtaaaacaa aaactattct taaacgcaag ctattcctat
      121 gcaagcggct tagaaaattt tgttccaaat agcgagtcgc cttttccgct tttcagtgct
      181 tcaaggcatt ctgtttcttg tagcatatct gctttttttt agcttacaga gtttgcgtta
      241 tattttctgg tgaaataaca agtgctgcac gagcccaagg aaattctttt tttataaaat
      301 cgtagttagc accactttca tctacctgtc ccctgccttc aaccaagaaa cctgtcccca
      361 tatagcgcag accctgaact tcgcgtgttc caacagtcat ttttaccttg gggtttattt
      421 caagattttc ttgtgtaatt cccattccac ccacaggtat aataattgat ttattgtcga
      481 cgatgcggat atacgtattc cacgtgtttg caatatgagc ttcattctgt gccaccgtta
      541 ctatggcagc aacaccctca tgtttgataa cctcaataaa agtttttgaa atttgcattt
      601 tatcctcctg aagaattatt aattgctaac actagattta attctacaaa aacctacttt
      661 cttggtaggc ttttgtgtct tgtaaactaa tataatacta aatttatttt ttagcaatag
      721 tccttcctct ctttgttttg tatactgcta atacagaaaa acaatgtaaa ccaaacgaag
      781 gaagcctatg aaatacgatt taataatagt tggcggtggc gctgcaggct tatatgcagg
      841 agcacagctt gcctcatatt gtgatagaaa aaaaaaggaa aataagacag aggcaaaaaa
      901 catagccatc atagacagct ctcctgaact gggtaaaaaa ctcttgctta ctggtgcagg
      961 gcaatgtaat ttgacgcaag ctggttctat aaaagatttt atttctcatt atggagagaa
     1021 tggctcgaaa atccgctctc ttttgtatgc acacaataat agcgatacaa tgcagttttt
     1081 tgaatccttg gggcttaggc tttttgaaag ggaggacgga aaggtttttc cacaaagctt
     1141 aagcgcaaaa gaagtgcgca atctcctttt ggctcgatgt aaaaaaaaca tagaaattca
     1201 aacaaataca aaactcatcg atataaaaat acttgcagaa aacactggat ttaaactaat
     1261 ccttgaaaaa gagaataaag aaattgtttt gcaaactcgt tttttattgc tttcttgtgg
     1321 aggtgcatcc taccccaaaa ccggctccga tggctccatt tttcccattc taaaaaaaat
     1381 gggactttcg gttgtgaaac taaaaccagc ccttgtacca ttttttgtag acaattatcc
     1441 attcaaagag cttgccggga tagccataga aagcgtgcat ataaaaaaag tcggcgaaaa
     1501 gatggcaaaa aaatcatttg agccattatt acttacccac gattcttttt cagggccaac
     1561 ggccttgcat ttttctcgtt acttagacca aggtgacaaa attttcttcg attatttgcc
     1621 ggcttgcaag gaagaagagc tagttgcaga agtgcaaacg aaagctttga ggaataacaa
     1681 gcaagtcgcg agcttttttt cgcaattgcc cgttttaaaa gaggcaggta ttccgaagcg
     1741 ctttgtggaa gtcatgtgcc aaaggtctag tatcgatcca acaacaaaaa tatccgctgt
     1801 ttctgggaaa aaactaaggg ctctcataca tttattcaaa tctgatgtat ttagggtgag
     1861 tggcacaaag gcttggcagc tcgctatgat tactgcagga ggagtgagcc ttgaagagtt
     1921 ggacttaaaa agcctttcca gtaaaaaata tccatccctc ttttttgctg gtgaaattct
     1981 tgatattgat ggagattctg gcggctataa tttacaattt gccttttctt cagcaaagaa
     2041 ggcgacagag agcataattg cctcttggga aaaataacaa taattttgat ttattcgact
     2101 taatctaccg caaagaacat tgcatattga caaaagtaaa gattttctct atagtataca
     2161 gaccttttaa ggagcgatgg ccgagcggtt gaaggcggcg gtcttgaaaa ccgttgtgct
     2221 gaaaggcacc gggggttcga atccctctcg ctccggttaa gaaaaatcaa ttgaatttct
     2281 atgtcaactt agtctgatag ggattcgatc cgaagcgacg gcgcgaccaa gcgggagcgc
     2341 aaatcgccac ggatggcgat ttaggagcgt aggcgggctg aaaggagaaa acaggacgtt
     2401 ttcgagtgaa agccgaatcc ctctcgctcc ggataagaaa aatcgattga atttctatat
     2461 caacttagtc tgatagggat tcgatttttt tggagcggtg cgtgagaggc cgaaacgggc
     2521 tccctgctaa ggagttgtgc ccctaaaggg caccgggggt tcgaatcccc cctgctccgt
     2581 actacgactt aaaaacatcc gtgtttttaa gtctttgaga gtttgtgaga caaactctca
     2641 ttatcccata agctaatggg gagacttcct tgtctcatca tttagcgaga gcaaggcgag
     2701 gaggaggcga caggacgtcg ccggggttaa acacactcgt tttggtttgc tttatatatt
     2761 gctccaccaa aacgagatag ttattttttt catggatgaa aaaaataacg tatacatccg
     2821 tgtttttaag tctttgagag tttgccctgc aaactctcat tatcccagta gcgaatgggg
     2881 taggtcttat gaaaactgaa tggatcaatg aggtgggaat tgactttgag gcttttaagt
     2941 gaaaattgcc ctgtaatgtt gattcccttt tttgtttgag actatagctc agctggatag
     3001 agcgtcagat tgcggatctg aaggccggtg gttcgaaccc acttagtctc g
//
LOCUS       DAOSSW010000120         3007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_29618_length_3007_cov_6.314363, whole genome shotgun sequence.
ACCESSION   DAOSSW010000120 DAOSSW010000000
VERSION     DAOSSW010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29618_length_3007_cov_6.314363"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..>1404)
                     /locus_tag="PKW26_07915"
     CDS             complement(55..>1404)
                     /locus_tag="PKW26_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HOQ93526.1"
                     /translation="YWDYTTAAIAEITDLIRVEIGDVQEINAKALAQQERSLFFVIIQ
                     FVIILVVVLVLSIIFTRLIRGPVLVIGKTLQAIASGEGDLTIESVVESSDEIGKLSES
                     FNKFTSTLSNMLKDIKHAVAGLESMGASLAEEMHQTASAENEVSTIIKSIGDKVLIQD
                     GEIEAAIASLNEFFKQLDSLHGLIESQAASVTESSASIEEMIASIRSEKLSVENMSRV
                     VEAMVSESNITFSQVKEVGEYIKEVDRQSELLLEANEVISSIADQTNLLAMNAAIEAA
                     HAGDAGRGFAVVADEIRKLAETSSEQSKEIASNLTKIKTVIDTVVSNTEKATDSFESM
                     NKSIANVTELQDAVLGSITEQSAGTNEVLIATTEINNITQKVRTMSADMDNHSKALQK
                     NLDGISQISSEVSAGMTETLIGMEEIHKSMLEVDKLSSTNKTNVEVVSNLISRFKLK"
     assembly_gap    1405..1504
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1505..2001)
                     /locus_tag="PKW26_07920"
     CDS             complement(<1505..2001)
                     /locus_tag="PKW26_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93527.1"
                     /translation="MFKSLSLRTKLLVLAAIPMLALFIAGVQSVYQDYMSWKIYDAQE
                     KNIAFYTANLNLLDALQSERGSASRYASGIVTKAELERYQAKTDELVSPWFENLRKAR
                     KNISSVKPRNVDAVTAASLDAVSTASTDASSAASATADATSSATYSDDDMDSLRESVD
                     GVERW"
     gene            complement(2035..2451)
                     /locus_tag="PKW26_07925"
     CDS             complement(2035..2451)
                     /locus_tag="PKW26_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOQ93528.1"
                     /translation="MKSYRKELHFNIPSRRGLVNISGEVQKAIDESGVKEGLVLVNAM
                     NITASVFINDDEGGLHNDYEKWLEKLAPEKPYSQYQHNGYEDNADAHLKRTIMGREAV
                     VAITKGQLDFGTWEQIFYYEFDGKRNKRVLIKIIGE"
     gene            2711..>3007
                     /locus_tag="PKW26_07930"
     CDS             2711..>3007
                     /locus_tag="PKW26_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93529.1"
                     /translation="MKHTHKLNSFLFIAPLCLFLSCVSAPPKAFHLQKAKKEKTATYF
                     CLPGSVKILQNEIPSHGYANEIELHSAKGEAESRQILISGGEKGLENISVSFQWE"
BASE COUNT          832 a          619 c          508 g          948 t
ORIGIN      
        1 ttgttatagt aaatgcaata cgtttgttag catacaagga gagaaaaagc tttcttattt
       61 caacttaaag cgagatatta aattactcac cacttcaaca tttgttttgt ttgttgagct
      121 tagtttatca acttcaagca tggacttatg tatttcttcc ataccgatga gggtttctgt
      181 catgcctgcg cttacctcgc tcgatatttg tgaaataccg tcgaggtttt tttgaagtgc
      241 cttactgtgg ttgtccatat cggcagacat agtgcgaacc ttttgcgtga tattatttat
      301 ctcagttgtt gcgataagca cttcgtttgt tcctgccgat tgttcagtaa ttgaacctaa
      361 aaccgcatct tgcagttcag ttacatttgc gatgctctta ttcatcgatt cgaaactatc
      421 ggttgccttt tcggtatttg aaacaacagt atcaataacg gtcttaattt tagttaaatt
      481 gcttgcaatt tcttttgact gctcgcttga agtttctgca agcttacgga tttcatcagc
      541 aacaactgca aagccgcgtc ccgcatctcc cgcatgagcg gcttctatgg cagcattcat
      601 agctaggagg tttgtttgat ctgcaataga agatattact tcgtttgctt cgagtaagag
      661 ttctgattgt cggtcaactt ccttgatgta ctctcctacc tcttttacct gcgagaaggt
      721 tatatttgac tcagaaacca tcgcttcaac aacgcgactc atgttctcta cagaaagttt
      781 ttccgagcga atgcttgcaa tcatctcttc aatacttgcg ctactttcag tgacgcttgc
      841 ggcttgtgac tcaattaagc catgtaagga atcaagttgt ttgaaaaatt cgttgagtga
      901 agcaatagct gcttctattt cgccatcttg aatcaaaacc ttatcaccaa tgctttttat
      961 aattgttgag acttcatttt cagcagaagc ggtttgatgc atttcttccg caaggcttgc
     1021 tcccatgctt tccaaacctg ctacagcgtg ttttatatct ttaagcatat tcgaaagagt
     1081 tgatgtaaat ttattgaagg actctgaaag cttcccgatt tcatcagaac tttcgacaac
     1141 agactctatc gttaaatctc cttcaccaga ggcaatcgct tgtaaggttt ttccaataac
     1201 aagtactggc ccacgtatga gccgtgtaaa gataatactc aaaacaagaa cgacaactaa
     1261 aattatcaca aactgaataa taacaaagaa gagggaacgt tcttgttgtg caagggcctt
     1321 tgcgttgatc tcttgtacgt cgccaatctc tactcgaata agatctgtta tttcagcaat
     1381 tgctgctgtt gtataatccc agtannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnatccat cgctcaacac catctacaga ttctcgtaaa gaatccatat catcatcgga
     1561 gtaagttgca gaagaagttg catctgcagt tgctgaagca gctgaagagg catcggtgct
     1621 tgccgttgac acagcatcaa gacttgctgc cgtaacagca tcgacattcc gcggctttac
     1681 agaagaaata ttttttcgtg cctttcgcaa gttttcaaac catggtgaaa caagttcgtc
     1741 tgttttagcc tgataacgtt cgagctcagc tttagtaaca ataccagaag catagcgact
     1801 cgcagatccc cgttcgcttt gtaatgcatc aagtaaattt agatttgcgg tataaaatgc
     1861 aatgtttttt tcttgggcat catatatttt ccaactcata taatcttggt atacagactg
     1921 cactcctgca ataaataaag cgagcattgg aatagccgcc aaaacaagta attttgttct
     1981 caagctaaga gacttgaaca tgttttctcc tcaatatttt tatacatagt tttcttattc
     2041 tccgattatt tttattaaaa ctcgtttgtt tcgtttcccg tcaaattcat aataaaaaat
     2101 ctgctcccat gttccaaagt caagctgccc ttttgtaatg gcaacaacag cctcccttcc
     2161 cattatcgtt ctttttaggt gcgcatcagc attatcttca tagccattgt gttgatactg
     2221 ggaatagggt ttttctggtg cgagtttttc aagccatttt tcataatcat tatgtaagcc
     2281 accttcgtca tcgttgataa acacagatgc ggttatattc atcgcgttga ccaaaaccaa
     2341 gccctctttt acccctgatt catcaatcgc cttttgaacc tctccgctga tatttactaa
     2401 acctcgacga gagggaatat taaaatgtaa ttctttacga tatgatttca tccgagaagc
     2461 cccctaaaaa atatatttta gcactggaat ataaaaacac aacaatgccc ctatttacat
     2521 cctatatcta gaaacagagg atatatcaat cattatataa tatctattgg aaatctgcaa
     2581 taaaaaatag gagtctgtca attctatggg aaaatttcgc cccttttata ctaatggtta
     2641 ctagcgaaaa ttttaccccc tgatgggtct ttttttacaa aacaagctat tttatgctat
     2701 acttatgcat atgaaacata cacacaagct aaacagcttt ctttttatcg ccccattatg
     2761 cctctttctc tcctgtgtaa gcgctccacc taaggctttt cacctacaaa aagccaagaa
     2821 agaaaaaacg gctacttatt tttgtcttcc gggaagtgtc aaaatacttc aaaacgaaat
     2881 cccttctcat ggatatgcta acgagattga actacatagt gcaaaaggag aagcagaatc
     2941 aaggcaaatc cttataagtg gtggagaaaa gggtttagaa aacatttctg tttccttcca
     3001 gtgggaa
//
LOCUS       DAOSSW010000121         2967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_30130_length_2967_cov_7.876374, whole genome shotgun sequence.
ACCESSION   DAOSSW010000121 DAOSSW010000000
VERSION     DAOSSW010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30130_length_2967_cov_7.876374"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..75)
                     /locus_tag="PKW26_07935"
     tRNA            complement(3..75)
                     /locus_tag="PKW26_07935"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Met,seq:cat)
     gene            complement(99..941)
                     /locus_tag="PKW26_07940"
     CDS             complement(99..941)
                     /locus_tag="PKW26_07940"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HOQ93530.1"
                     /translation="MNLATFVLLFFIYSGIGYISEVIYCSARERKFVNRGFLYGPLCP
                     IYGFGGLLVVFLLQPFSYSFPLLFLMAVLVTTILEYVGSWVLETLFDMKWWDYSDYKL
                     NINGRVCALNSVLFGLMGIIAVYWIHPLVLTMIENLKPLVQQSIASVLLIAFMIDVLF
                     TLRSLIHFVDMLARLSEVIQRARVSIDNTLWFNEKDLRGSLQNLREKMKKNALDDYRV
                     LEKHLSQFIKQAKSTKRLFAAFPRMRSKYRNHQLSLLKRFHKKTRAIEESDKTIDKDL
                     TKTP"
     gene            complement(978..>2967)
                     /locus_tag="PKW26_07945"
     CDS             complement(978..>2967)
                     /locus_tag="PKW26_07945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOQ93531.1"
                     /translation="FLDTDGKTLWIGTFGGLAKLDTKTGFISTWSHEPTRSESLCNNT
                     VVSIGRDAEGRLWVGTLDGLDRKEGDTFIHYRTGKNKEGKIESSLIRSLLLDSRGDFW
                     VGTGEGGFYKYLLETDSFKAYRKSNLPGKEIFSDQVFSIKEDQDGYLWLGLWGFGLTR
                     FDTKTEETKNYPLADSRVYFVNTEEAGFVRAGTWGGGYFELATDSEEIKQFRKDDDSL
                     WSLSNNTVYSMLYDTAGNTWVGTNGGGFALVLKESPAYSLYEHDPKKTGSRSSGRTLS
                     ILEDSKNRLWIGSYNTGLNRLDPGETDFVHYVHNPENRKSLPNDIVGKVFEDSKGIIW
                     IATNQGLARYNEEDDNFDVILHDPQNPFSLADNTVQNIIEEKETGNLWFATYNKGLEY
                     WNREENIFYHFAHDSENPTSISNNLTLALAYDAQNRLWVGTSQGLCRYEGDGIFTRYV
                     MDMHNPRSLPSSNIRWLHTSSDNCLWIATDSGGIAVYDEESDSFSHWTKKDGLTGVNN
                     RRALTTELKQRFEEAVLLFEPIAALMIDIDFFKLYNDYYGHQKGDETLIAVARTLKNS
                     LDRPKDSVSRYGGEEFIILLPSTDHVGAKVIAERMRLAVENLEIPHMVSSVKKNITIS
                     VGYASYIPRRGEEMTTIIDMADRALYRAKDAGRNCISS"
BASE COUNT          899 a          649 c          487 g          932 t
ORIGIN      
        1 gttagcagga gtaggactcg aacctacgac ctccgggtta tgagcccgac gagcttccag
       61 ctgctctatc ctgcgtcgtg ttatattact gtacttcttt acggcgtttt agtcaagtct
      121 ttgtctatcg ttttatcact ttcttctatt gcacgagtct ttttatgaaa gcgttttagt
      181 aaagaaagtt gatggttgcg atatttactt cgcattcgag ggaaggctgc aaaaaggcgt
      241 tttgtgcttt ttgcctgttt aataaactgg gataaatgtt tttccaaaac acgataatca
      301 tcaagtgcat tttttttcat tttttcacga aggttttgta aactgccccg taaatctttt
      361 tcattaaacc acaatgtatt atcaatcgaa acacgagctc tttgtatcac ttcactcaat
      421 cttgcaagca tatctacaaa atgaataaga gatctcagtg taaataacac atctatcata
      481 aatgcgatta ataatacgct tgcaattgat tgctgtacaa gtggtttcaa attttcaatc
      541 attgtcaaaa cgagtgggtg tatccaatat accgcaatga ttcccattaa accaaataaa
      601 actgaattaa gagcacacac acgtccatta atattgagct tataatccga ataatcccac
      661 cacttcatgt caaacaatgt ttctaggacc caactaccca catattccaa aatagtagtg
      721 accaaaacag ccattaaaaa taaaagtggg aaagagtatg aaaaaggctg taataaaaat
      781 acaacaagaa gtccaccaaa accataaata gggcaaagtg gaccatataa gaatccacga
      841 tttacaaatt ttcgttctcg tgctgaacaa tagataactt cagaaatata accaatacca
      901 ctgtaaataa aaaataaaag aacaaaagtt gcaagattca tatttttata ctcctcttct
      961 ataacaacaa attttaccta acttgaaata cagttccttc cagcatcttt tgctcgatac
     1021 aaggctctat ctgccatgtc gattatcgta gtcatttctt ccccacggcg aggtatatat
     1081 gacgcataac caacactaat cgtaatattt tttttaacac tcgaaaccat atgcggaatt
     1141 tctaggtttt caactgcaag tctcatgcgt tcagctatca cctttgcgcc gacatgatct
     1201 gtgcttggca aaaggatgat aaattcttcg ccaccgtatc gactcacact atcttttggt
     1261 cgatcaagtg agttttttaa agtccttgca acggctataa gggtctcatc gcctttttgg
     1321 tggccataat aatcattata taatttaaag aaatctatat ctatcataag agctgcaata
     1381 ggctcaaaaa gtaaaacagc ttcttcaaat ctttgtttca actctgtagt caaagctctt
     1441 ctattattta caccggtaag tccatctttt tttgtccagt gtgaaaacga atcactttct
     1501 tcatcataca ctgcaatgcc accgctgtct gttgctatcc acaaacagtt atcagaactc
     1561 gtgtgaagcc agcgaatatt tgaagaggga aggctacgtg gattatgcat atccatgaca
     1621 tagcgtgtaa aaataccgtc tccttcatag cggcaaagtc cctgacttgt tcctacccac
     1681 aatcgatttt gtgcatcgta tgcaagcgct aaagttaaat tgttgctaat cgaagtggga
     1741 ttttctgaat catgcgcaaa gtgataaaag atattttctt cacggttcca atattcaaga
     1801 cccttgttat aggtagcaaa ccataggttc cccgtttctt tttcttctat tatattttga
     1861 acggtattat ctgcaaggct aaacggattt tggggatcat gtaagataac atcgaaatta
     1921 tcatcctctt cgttgtacct tgcaagcccc tgatttgttg caatccaaat aattcctttc
     1981 gagtcttcaa aaaccttacc gacgatatca tttggcagac tctttcgatt ttcaggattg
     2041 tgcacataat gcacaaagtc tgtctctccg gggtcaagtc gatttaggcc agtattatac
     2101 gatccaatcc ataggcgatt tttgctatcc tctaaaatag aaagcgtcct gccgcttgaa
     2161 cgcgagccag tttttttggg gtcatgttca tacaatgaat acgctgggct ttcctttaaa
     2221 acaagcgcaa agcctccgcc atttgtacca acccaggtat tgcctgccgt atcatagagc
     2281 atggaataaa cagtattgtt tgataaggac caaagactat catcatcttt tctaaactgt
     2341 tttatttctt cgctatctgt ggctagttca aaatatccac caccccaagt gccagctcgc
     2401 acaaaacctg cctcttctgt atttacaaaa tacacacgag aatctgcaag gggatagttt
     2461 tttgtctctt ccgtttttgt atcaaatcgt gtaaggccaa aaccccagag tccaagccat
     2521 aaatatccat cttgatcttc ttttatagaa aaaacttggt ctgaaaaaat ttctttgcct
     2581 ggaagatttg atttcctata ggctttaaaa gaatctgttt ctagaagata tttataaaac
     2641 ccaccttctc ctgtgcctac ccaaaaatca ccacgagaat caagcaaaag tgaacgaatt
     2701 aaactcgact ctattttccc ctccttgttt tttcccgttc tgtagtgaat aaaagtatcg
     2761 ccttcttttc tgtcaagacc gtcgagagta ccaacccata gccgcccttc cgcatctcgt
     2821 ccgattgaca caacagtatt gttacaaaga ctttctgatc ttgtcggttc atgcgaccaa
     2881 gtcgatataa aacctgtttt tgtatctaat ttagccaagc cgccaaaagt tcctatccac
     2941 agggttttgc catctgtatc taaaaaa
//
LOCUS       DAOSSW010000122         2929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_30671_length_2929_cov_6.572721, whole genome shotgun sequence.
ACCESSION   DAOSSW010000122 DAOSSW010000000
VERSION     DAOSSW010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30671_length_2929_cov_6.572721"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            327..617
                     /locus_tag="PKW26_07950"
     CDS             327..617
                     /locus_tag="PKW26_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93532.1"
                     /translation="MAQRRRHDWPALFEEQKNSGKTVVQFCEEKQIHQNLWYKHKQKM
                     KPGFIEISNKTEKAVHEGMQIITGDFEIHIKAGCNEERLVSLVKYLGMHNVL"
     gene            607..954
                     /gene="tnpB"
                     /locus_tag="PKW26_07955"
     CDS             607..954
                     /gene="tnpB"
                     /locus_tag="PKW26_07955"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HOQ93533.1"
                     /translation="MFFDMSNVKIFIRPGHTDLRKAINGLSILIQEEMECDPFSGSLY
                     LFCSKNKRLLKIIYWDKNGFCLWQKRLEKHTFPWPKSEEDVLEIDYEKIQMLLKGIDF
                     WQAHTTLAYKSVV"
     gene            1003..2535
                     /locus_tag="PKW26_07960"
     CDS             1003..2535
                     /locus_tag="PKW26_07960"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HOQ93534.1"
                     /translation="MNIETLPCAVQEYIHNLIQEKNKLEIKYQILEEKYTLVLFQKFC
                     KKAETVSADQLALFEEESEQTKAPEEKDETVTIKSYTRKKTGRKAIDESLPRQIIMHD
                     ISEEEKTCACGCALKCIGEESSEKLNVIPEQMWVEKHVRPKYACPSCEGTADEESPAV
                     KIAPVEKTILPKSIATPGLLSFILVNKFVDHLPYYRQEKRFERIGVHISRQDMCNWQI
                     KAMHALEPLFVLLKKQMKNGSVLNMDETTVQVMNEAGRSDTQKSYMWLCTGGSEGKKV
                     FWYEYKPTRAASHIHSFLENFSGYLQTDGYQAYETAMKEYPDIKHVGCFAHARRKFFE
                     ASKGMKNPASPMEGIKWIKKLYDVEDHLRIKYTDANEFLLQRKKETEPILSGFKSWLD
                     KKALHTAPQGLMGKAIFYTLGQWEKLIAYLEHKDLTPDNNISENAIRPFVVGRKNWLF
                     SGSPTGAETSAKVYTLVETAKHCKVNLHDYLRIVFEKAAHANTEEDWNKLLPWNLFQD
                     DG"
     gene            complement(2601..>2929)
                     /locus_tag="PKW26_07965"
     CDS             complement(2601..>2929)
                     /locus_tag="PKW26_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93535.1"
                     /translation="RKNISLSAFIFSGIYPCPGAVLVLILSFTLNIIKLGITSVLVMS
                     LGMSIPILISAYIAWAGREGLFYFFKNKEGIISKLSFIIELLGYALLLAFSFYIAWPF
                     LIGLLR"
BASE COUNT         1066 a          505 c          581 g          777 t
ORIGIN      
        1 acacattgat aacatctgca aacctttcct atcacggaca agaaggaaat attgagctag
       61 gtttatatgt agaatcaagg gacatcgcta cccaactaga tgatattttt acaaatttaa
      121 tttttttgaa aatctttcat caatattaac aaactatttc cgagtttatt catgtaaatt
      181 agaaagtggt tattgataaa taattttgta ataaaacgat ttgaaaaaac aaccaaatgt
      241 ttgtatccgt caaataaacc gcatcttcta atccatttca aatgatatta tactgcatct
      301 ccagaacaat agtaaggaga aaaaatatgg cgcaaagaag aagacatgat tggcctgcgc
      361 tttttgaaga gcaaaaaaat agtggaaaaa cggtggtgca attttgtgaa gaaaaacaga
      421 tacaccaaaa tctttggtac aaacacaaac agaaaatgaa acccggcttt atcgaaattt
      481 ctaacaaaac agaaaaggca gtacatgaag gaatgcaaat tataactggt gattttgaaa
      541 ttcacattaa agctggctgt aatgaagagc ggcttgtgtc cttggtaaaa tatttaggaa
      601 tgcataatgt tctttgatat gtcaaacgta aaaatcttta tccgaccagg gcatactgat
      661 ttacgaaaag caataaatgg attatcaatt ctcatacaag aagagatgga gtgtgatcct
      721 ttttcgggga gtctgtattt attttgtagc aaaaacaaac gccttttgaa aataatctac
      781 tgggataaaa acggattttg tttgtggcaa aagcgtcttg aaaaacatac ttttccgtgg
      841 ccaaagagcg aagaggatgt tttagaaata gattatgaaa aaatacaaat gcttctcaag
      901 ggtattgatt tttggcaggc gcatacaaca cttgcatata aatcggtagt atagctcata
      961 aaagaatgta cattatttgt aaaaattgat acactctttt ttgtgaacat agaaacatta
     1021 ccatgcgcag tacaagaata tattcataat cttattcaag agaaaaataa gttagaaata
     1081 aaatatcaaa ttcttgaaga gaaatataca ttagttttat ttcaaaagtt ttgtaaaaaa
     1141 gcagaaactg tttcagcaga tcagcttgcc ctttttgaag aagaatcaga gcaaacaaaa
     1201 gcgcccgagg aaaaagacga gacagttact atcaagtcgt acacaagaaa aaagacggga
     1261 cgaaaagcaa ttgacgaatc acttccaaga caaatcataa tgcacgatat tagtgaagaa
     1321 gaaaagactt gtgcctgtgg ttgtgctctt aaatgtatcg gagaagagag tagtgaaaag
     1381 ctcaatgtta tccccgagca gatgtgggtt gaaaaacatg tgcgcccaaa atatgcctgc
     1441 ccttcttgtg agggaactgc cgacgaagag agtcctgcgg taaaaattgc accagttgaa
     1501 aaaacaattc ttccgaaaag tattgcaaca cctggactct tatcgtttat attggttaat
     1561 aaatttgtag atcacctgcc atactatcga caggaaaaac gatttgaaag aatcggcgta
     1621 catatcagca gacaggacat gtgcaactgg caaataaaag caatgcatgc tcttgagccc
     1681 ttgtttgtac ttttaaaaaa gcaaatgaaa aatggaagcg ttctaaatat ggatgagacg
     1741 acggttcaag ttatgaatga agctggtcgc tctgatacgc aaaaatcata catgtggctt
     1801 tgcactggtg gcagtgaagg gaaaaaagta ttctggtatg aatacaaacc gactcgagct
     1861 gcctcccata ttcatagctt ccttgaaaat ttttcgggct accttcagac agatggatat
     1921 caagcctatg aaacggcgat gaaagaatat cctgatataa agcacgttgg ttgttttgct
     1981 catgcgaggc gaaagttttt tgaagccagt aaagggatga aaaatcctgc aagcccgatg
     2041 gaaggaatta agtggataaa aaaactctac gatgttgaag atcaccttcg cataaaatac
     2101 acggatgcga atgagttttt attacagcga aaaaaagaga cagagcctat tctttcaggc
     2161 tttaagtctt ggcttgataa aaaagcactt catacagcac cgcaaggact catgggtaaa
     2221 gcaatttttt atacactcgg tcaatgggaa aaattgattg cctatttgga acacaaagat
     2281 ttaacacctg ataataacat aagcgaaaat gcgattcgcc cctttgttgt cggcagaaaa
     2341 aattggcttt tttccggtag cccaacaggt gccgaaacat cagcgaaagt atatacgcta
     2401 gtcgaaacag caaaacactg caaagtaaac ctgcatgact atttacgaat tgtctttgaa
     2461 aaagcagccc acgcaaatac cgaagaggac tggaacaaac tacttccttg gaacttattt
     2521 caagatgacg ggtagtttat ttgacggata caaatgtttt cctcaatcct acctccacag
     2581 aatccccccc ctcccgctat ctaccgcaat aagccgatta aaaaaggcca ggctatataa
     2641 aatgaaaagg caagaagtag ggcgtagcca agtagttcta taataaagct gagctttgaa
     2701 ataatgcctt ctttattttt gaaaaaataa aagagtcctt ctcgtcctgc ccaggcaatg
     2761 taggccgaaa tgagaatggg gatgctcatg cccaaggaca tgacgaggac tgaagttatg
     2821 ccgagtttta ttatattcaa tgtaaaagac aaaattaaaa caagcactgc gccagggcag
     2881 gggtagatgc cagaaaaaat aaatgcgcta agggaaatgt tttttctac
//
LOCUS       DAOSSW010000123         2838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_31990_length_2838_cov_10.641035, whole genome shotgun
            sequence.
ACCESSION   DAOSSW010000123 DAOSSW010000000
VERSION     DAOSSW010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31990_length_2838_cov_10.641035"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1984)
                     /locus_tag="PKW26_07970"
     CDS             complement(176..1984)
                     /locus_tag="PKW26_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOQ93536.1"
                     /translation="MATKKNTQNHEEMQTCFTPLALSSLDDFFKSYDYFSKDERTRLQ
                     TAYLYLLEKTKNLHRSSGEPYFEHPYRVAEILAQSKLDADSIIAAFFHNIFELNIVSE
                     SEIESLFGKTVLNITKGTAKITSMQIKSKTMQEAASFRKMLFAMVDDIRIILVKMADR
                     LDRMRHLKEVSAESQKKIAQEVIDIWAPLANRLGMSNVKSELEDLSLKYTNPDVFQQL
                     KQLVALKKSERSEYLLLAQKEIYSLANTHGIEVAIRSRAKHFYSIYQKMKKRNKAAED
                     MYDLLALRILCETNADCYSLIGLVHTLWKPLEGRFKDYIAMPKANGYQSLHTTVMSHG
                     HPLEIQIRTHKMHEVAEHGVASHWLYKQGLSANNAALENLSIINQLKELKNENTQNEQ
                     AFNELRDELLGDSIFVFTPKGDVRELPQGATAIDFAYSIHTRIGETITGAKANGAIIP
                     LSKPLKNTQVIDIITSPKAHPTWNQLRLVKTQKARSKINAYLNENDQLRIQAEKQAAL
                     EKQQAQERQAASLKEKAAKKELREADENRDALKIRIGDTSNFLVTLARCCNPSYGDAI
                     VGYVSRGRGLIVHQAGCRNFSRIPNVNERSVEVLWD"
     gene            complement(2102..>2838)
                     /locus_tag="PKW26_07975"
     CDS             complement(2102..>2838)
                     /locus_tag="PKW26_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOQ93537.1"
                     /translation="ALTLIKKSKNILVFTGAGISTGSGILDFRSENGLYSLLSKNTDL
                     PYPEAIFDIKYFKKNPKPFFSLAKNMLEQHAKPTNCHKLLAELEKNNQISLVLTQNID
                     MLHQAAGSKKVLECHGSFMSAHCLDCHSAYNLNDYKQRVLDDSIPYCNCGGIIKPDIT
                     FFGESLPSAFYDLVNNPPETDLVLILGSSLNVQPAAGFALEMAMKVPSILVNLAATPY
                     DNAISLVYHKDLEDFAEYILRDNLIT"
BASE COUNT          801 a          526 c          561 g          950 t
ORIGIN      
        1 ttgcataata caggtgaatt attaattatg aatgaagatt ttcactacgt gttttcgaga
       61 atttttagag aaataacacg taaatcaacc accattacgt gctttttcca gattttaact
      121 caaaaaacac gtaactgagt gataaacatc taaattcata attttatttt ctatgctagt
      181 cccagagcac ttcgacggaa cgttcgttca cgttgggaat tcgagaaaag tttcggcagc
      241 ctgcttgatg gacgatgaga ccccgcccac gtgaaacata accgacaatt gcatctccat
      301 aacttggatt acagcaccgt gcaagagtta ccaaaaaatt ggacgtgtcg ccgatgcgaa
      361 tttttaaagc atctctattt tcatctgctt ctcgcaattc tttttttgca gctttttctt
      421 ttaagcttgc agcttgcctt tcttgtgctt gttgtttttc gagtgctgct tgtttttcag
      481 cttgtatacg aagctgatca ttttcattga gatacgcatt tattttactt cgtgcttttt
      541 gcgtttttac caatctcaat tggttccaag ttggatgtgc ttttggagaa gtaataatat
      601 cgattacttg tgtatttttc aaaggttttg aaagaggaat aatagcacca tttgcttttg
      661 caccggtaat ggtttcgcca atcctcgtgt gaatgctata cgcaaagtca atcgctgttg
      721 ccccttgcgg aagttcgcgc acatctcctt ttggcgtaaa aacaaaaatc gaatcgccca
      781 aaagttcatc acgtagttca ttgaatgctt gttcattttg agtgttttca tttttcaatt
      841 cttttaattg attaataatc gataagtttt caagtgccgc attatttgca gaaagccctt
      901 gcttataaag ccaatgactc gcaacaccat gttcagcaac ctcgtgcatt ttgtgagttc
      961 gaatttgaat ttcaagcgga tgcccatggc tcataactgt cgtgtgcaaa ctctggtatc
     1021 cattcgcctt tggcatggcg atataatctt tgaatcttcc ttcaagtggt ttccaaagtg
     1081 tatgcaccaa accgatgagg ctataacaat cagcatttgt ttcgcagagg atacgcaagg
     1141 cgagtaagtc gtacatatct tcggcggcct tgtttcgttt tttcattttt tgataaatag
     1201 agtaaaaatg ttttgcgcga cttcgaatag caacttcgat gccgtgcgta tttgccaggc
     1261 tgtaaatttc tttttgtgcc agcaaaagat attcgctcct ctctgatttt ttgagggcga
     1321 cgagttgctt gagttgttga aatacatcgg ggttggtgta ttttaaactc aagtcttcaa
     1381 gctcgctttt tacgtttgac atacccaatc gatttgccaa aggagcccaa atgtcgatta
     1441 cctcttgggc aatttttttt tgcgactcag cacttacttc tttgagatgg cgcatgcgat
     1501 cgagacggtc agccattttt acaaggataa tgcgaatgtc atcgaccata gcaaaaagca
     1561 tttttctaaa actcgccgcc tcttgcattg tcttgctttt tatttgcatg ctcgtaattt
     1621 ttgccgttcc cttggtgata tttaaaactg tcttaccaaa aagactttct atttcacttt
     1681 cactgacgat attgagttca aaaatattgt gaaaaaaagc cgcgataata gagtccgcat
     1741 cgagtttgct ttgcgctaaa atttctgcta cacgataggg atgctcaaaa tagggctcgc
     1801 ccgaagaacg atgcagattt tttgtttttt caagtaaata caaataggcg gtttgtaaac
     1861 gagtgcgctc atcctttgaa aaataatcat agcttttaaa aaaatcatcg agagaactga
     1921 gggcgagagg agtaaagcac gtttgcattt cttcgtgatt ttgtgtattt ttcttcgtag
     1981 ccatagctac aatatacact ttttgtattt aaaatactag tatttaaatc atagttgtga
     2041 gctatgaaat gggagaattg aggtgggaaa atcgaaataa aatatgtaac gaagtttcgg
     2101 tttatgtaat gagattatct cttaaaatat attctgcaaa atcttctaaa tctttgtgat
     2161 agaccaaaga aatagcattg tcatagggag tggcagccaa atttacaagt attgagggta
     2221 ctttcatcgc catttcaagg gcaaaacctg ctgccggctg cacatttaaa gaagagccga
     2281 gaattaatac taaatctgtt tcgggtggat tattgactaa atcataaaaa gcagaaggta
     2341 agctctcccc aaaaaaggtg atatctggtt taataattcc gccgcagttg cagtagggga
     2401 ttgagtcatc taaaaccctt tgtttataat cgtttaagtt gtacgcagaa tggcaatcta
     2461 gacaatgcgc tgacataaaa gagccatgac attcgagtac ttttttagac ccagcggctt
     2521 gatgaagcat atcgatattt tgcgtgagga caagggagat ttgattgttt ttttctagtt
     2581 ctgcgagtag tttatgacag tttgtaggtt ttgcatgctg ttcaagcatg tttttggcaa
     2641 gactaaaaaa tggtttggga ttttttttga aatacttgat atcaaaaatc gcctcaggat
     2701 agggcaagtc agtattttta ctgagcaaag aatagagacc attttcacta cgaaaatcaa
     2761 gaatcccact acccgtgctg attcccgccc ctgtaaacac aaggatattt ttcgattttt
     2821 taattaatgt aagagctt
//
LOCUS       DAOSSW010000124         2823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_32234_length_2823_cov_7.899566, whole genome shotgun sequence.
ACCESSION   DAOSSW010000124 DAOSSW010000000
VERSION     DAOSSW010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32234_length_2823_cov_7.899566"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKW26_07980"
     CDS             <1..224
                     /locus_tag="PKW26_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698769.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOQ93538.1"
                     /translation="EDKIKIQRYKGLGEMNGDQLWETTMDPARRKMMRVHLDDAVEAD
                     RIFTTLMGEHVEPRRKFIEENAMYAQLDV"
     gene            249..2693
                     /gene="gyrA"
                     /locus_tag="PKW26_07985"
     CDS             249..2693
                     /gene="gyrA"
                     /locus_tag="PKW26_07985"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HOQ93539.1"
                     /translation="MSKEIQTEAGGTLIPISIEDEVKKAYIDYSMSVIVSRALPDVRD
                     GLKPVHRRILYSMEEMGLRYTTPTKKCARIVGDVLGKFHPHGDASVYDALVRMAQDFS
                     LRYTVVEGQGNFGSVDGDPPAAMRYTEAKLAKIADPMINDIKKETVDFVPNYDDSMVE
                     PSVLPAAFPFLLANGSSGIAVGMATNMPPHNLREVATAIGAYIDNPEVSIDELSKIVK
                     GPDFPTGGIIFGRKGIRQAYKTGRGRVIMRGRFTIDIDKKGKETIIFTEIPYQVNKAN
                     LITRIGELVRDKGIDGIANINDFSDRNGMKISIELKRGAIAKIVLNQLFTKTALQSTF
                     GVINLALVKGRPETLTLKQLIQYFVEHRQEVVTRRTAFDLRKAEERAHILRGLIKAIQ
                     NIDEVIKIIKASRNIETAKNALKERFDFSDIQAQAIVDMRLGRLTSLEIEKLEKELEE
                     LEVLIAHLQDLLAHPEKILALIKDETNEIAEKFGDDRKTDIISDEVEEINIEDLIKNE
                     EMVILISNLGYIKRVPVTAYRTQGRGGKGSASTKLVEDDFINQVFIASTHDHIMFITN
                     EGKLYWVKVHEIPEATRNSRGSHIKSLLPMSQNEEITTVVSLTEFSEDQFLFMATAGG
                     IVKKVRTIDFQNAKTRGIIAIRLDEGDKLVSSILSSGEDELVLITRRGQALRVSETEV
                     RPMGRASRGVIGIRLAQGDELAGAIHVEDDAKMLILTEFGCGKQVEFSEFTPHGRGTG
                     GQRIYRISEKTGEIVGTKTIGLGAELVVITSQGKTLRLDSANIPTQGRTAAGVCVFTL
                     DKPDMVIGVDVVAQEE"
BASE COUNT          882 a          496 c          644 g          801 t
ORIGIN      
        1 atgaagataa gataaaaatt caacgctata agggtctcgg tgaaatgaat ggagatcaac
       61 tttgggaaac aacaatggat ccagctcgtc gaaaaatgat gcgtgtgcat ctcgatgatg
      121 cggtagaggc ggatagaatt tttacaacgc ttatgggtga acatgtagag ccaagaagaa
      181 aatttattga agaaaatgcg atgtacgcac aattagatgt ttaatgcatt ttagtgtagg
      241 agttttttgt gtctaaagaa atacaaactg aagcgggggg aaccctcatt cccatttcga
      301 tagaagatga ggtaaaaaaa gcctatatcg actattctat gtctgttatt gttagtcgag
      361 ccttaccgga tgttcgagat ggtttaaaac cagtacatag acgaattctt tattccatgg
      421 aagagatggg tttacgttat acaacgccta caaaaaaatg tgcgcgtatt gttggggatg
      481 tacttggtaa atttcacccc catggagatg cttctgttta tgacgccttg gtgcgtatgg
      541 cacaggattt ttctctgcga tataccgttg tagaagggca gggaaacttt ggctctgtag
      601 acggcgatcc tcctgcagct atgcgttata ccgaagcaaa gcttgcaaaa attgcagacc
      661 ccatgataaa tgatataaaa aaggaaaccg tagattttgt tccaaactac gacgattcta
      721 tggtggagcc ttcggtttta ccagcagctt ttcctttttt actggcaaat ggttctagcg
      781 gtattgccgt tggtatggct acgaatatgc caccacataa cttgcgagaa gttgcaaccg
      841 ctatcggcgc ctacatagac aatccagaag tgagcataga cgaactttcc aaaatagtta
      901 agggtccaga ttttcctact gggggtatta tttttgggcg aaaaggtatt cgacaagcct
      961 ataaaacagg gcgcggtcgc gttattatgc gtgggcgctt tacaatcgat atcgataaaa
     1021 aaggcaaaga gacgattatc tttactgaaa ttccctatca agtaaataag gcaaatttaa
     1081 taacacgaat aggggaactt gttcgtgata agggcattga tggaattgcc aatataaatg
     1141 acttttctga ccgcaatggt atgaaaattt ctatagagct aaaacgtggt gctattgcaa
     1201 aaattgtttt aaatcaattg tttacaaaga cagctttgca atcaaccttt ggggttatta
     1261 accttgctct cgttaaaggg cgaccagaaa cacttacgct taagcaattg attcagtatt
     1321 ttgtggaaca tcgccaagaa gtagttacac ggcggaccgc ctttgatttg agaaaggctg
     1381 aagagcgggc acatatttta cgtggactta taaaggcaat acaaaatata gatgaagtta
     1441 ttaaaattat aaaagcttct aggaatattg aaacggcaaa aaatgcctta aaagagcgct
     1501 ttgatttttc cgacatacaa gctcaggcga ttgtcgatat gcgactcggt cgtcttacaa
     1561 gcttagaaat agaaaaacta gaaaaagaac ttgaagagct tgaagttctt attgcccatt
     1621 tacaagatct tttggcgcat cctgaaaaaa tacttgctct cataaaagat gaaacaaatg
     1681 aaattgccga aaaatttggt gatgatagaa aaacagatat tatttccgat gaagttgaag
     1741 aaataaacat agaagattta ataaaaaacg aagaaatggt tatccttatt tctaacctcg
     1801 gttatattaa acgtgttcct gttactgcct atagaacgca aggtcgcgga ggaaagggct
     1861 ctgcgagcac aaaactcgta gaagatgact ttattaacca agtttttatt gcttctactc
     1921 atgaccatat aatgtttatc acgaatgaag gaaaattgta ctgggtgaag gtgcatgaaa
     1981 tacctgaagc aacacgaaat tctcgcggtt cacatataaa aagtttgttg cctatgtctc
     2041 aaaacgagga gataacgact gttgtttctc ttactgagtt ttctgaagat caattcttgt
     2101 ttatggcaac tgctggtgga attgtaaaaa aagtgagaac aattgatttt caaaatgcaa
     2161 aaactcgtgg cataatcgct attcgccttg atgaagggga taagcttgtt tcatctatct
     2221 tgagtagtgg tgaagatgag cttgtgctta taacccgccg agggcaagcg cttcgtgttt
     2281 ctgaaacgga agttcgtcca atgggtcgag catctcgcgg cgttattggc atacgtttag
     2341 cccaagggga tgagcttgct ggagcaattc atgtagaaga tgatgcgaaa atgcttattc
     2401 tcactgaatt tggctgtgga aagcaggttg agttttctga atttacccct cacggtcgag
     2461 gcacaggcgg acaaagaatc tatcgaataa gcgaaaagac aggcgaaatc gttggtacaa
     2521 aaacaattgg tcttggcgct gagcttgttg ttattacaag tcagggaaaa accttacgcc
     2581 tagattcagc aaatattcca acacagggga gaacagctgc cggagtttgt gttttcaccc
     2641 tcgataaacc agatatggta attggcgtag atgtcgtggc tcaggaggaa taaagctttt
     2701 tgacctccct gtcaaaaagc tttttacaga aatcgtttgt gttttgcttc cgatttctgt
     2761 ctcccgtagg cgctttgaaa gcgcagcgtc cgtgctgcga gttgttccac gtgaaacatt
     2821 ttg
//
LOCUS       DAOSSW010000125         2817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_32319_length_2817_cov_5.689718, whole genome shotgun sequence.
ACCESSION   DAOSSW010000125 DAOSSW010000000
VERSION     DAOSSW010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32319_length_2817_cov_5.689718"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..305)
                     /locus_tag="PKW26_07990"
     tRNA            complement(233..305)
                     /locus_tag="PKW26_07990"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(270..272),aa:Lys,seq:ctt)
     gene            complement(319..392)
                     /locus_tag="PKW26_07995"
     tRNA            complement(319..392)
                     /locus_tag="PKW26_07995"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(356..358),aa:Arg,seq:tcg)
     gene            complement(414..485)
                     /locus_tag="PKW26_08000"
     tRNA            complement(414..485)
                     /locus_tag="PKW26_08000"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:His,seq:gtg)
     gene            648..1139
                     /locus_tag="PKW26_08005"
     CDS             648..1139
                     /locus_tag="PKW26_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93540.1"
                     /translation="MGNDILNAKRLYDVVLAEIELMSQIIQMQKAVREATKNRDWESL
                     QSTFYYINELSEGFLELEERRVAYFKDFGAKTGSELHQISQNLPFQFKNPITSVFTEL
                     KKKLLESKIENDAINEYISITQEFIQGVFDEVLPQRRNTLYSKTGTLIKNQPESIILS
                     AVL"
     gene            1152..>2817
                     /gene="flgK"
                     /locus_tag="PKW26_08010"
     CDS             1152..>2817
                     /gene="flgK"
                     /locus_tag="PKW26_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOQ93541.1"
                     /translation="MSTFAGIELGKRSLMAHSQQINTAGHNISNAETEGYSRQRVHIR
                     AYDPLYRPDLNRAETPGQIGQGSSIESIARVRDSLLDARITSQSHLEGYWSKRESYYQ
                     MLESIYNEPVDVSIRSTMDAFWEAWQELSLYPESKAARQAVVTRSESFIDSVKQRHDG
                     LVGVGTLLNGDIEATVEQVNSYTRDIAALNKEIVRSRAMGDNPNDLLDRRDLLVDKLS
                     SIINITTDERDPDEFMVHLDGKVLVQGNIARNFSVEAIKDGTGYTKVIWADTKEDAFF
                     NGGSLGGLIELRDVDVREELQSLNTFVMNFADLVNDIHRNAVGANNTTGLDFFVEQPF
                     VTNALGNYDRDSDGVDDSSYIFRMTGGNTLNPQDKVGFDGTIRLASRTGLVEISYYAT
                     DTVETIIDKINDSESEVKAYLDRNSHLVLKATTAANPENPDFVIRHVEDSSYFLSQYT
                     GLLAESGEEGAYRFDQANAVNALVEDKARYAVAPVLNPSAYFTVNEAIKKDVLSVASA
                     FPTSSGLAEVGDGSAALAIASLRNGMIMIGENKSFDDYFSETVTKIG"
BASE COUNT          886 a          576 c          597 g          758 t
ORIGIN      
        1 cggtcaaagg atttttatac gagacataat aatttggata atttgcacgc ttagatatac
       61 aaaagggcat aataccacct ctaaaacgtg taccaccagg ctctttttta gcaaaaagcc
      121 tgtggatttg agtgtgtttt cttcattgca cactcattgc acattttttt tcagttctat
      181 tacgcccttt gtgagggtag tatatttctt tataacataa agaattagat gctgagccat
      241 acaaggatcg aacttgtgac ccacagatta agagtctgtt gctctaccag ctgagctaat
      301 ggcccaaaaa agtaatctcg cgtccggcac gattcgaacg tgcgacctac ggattcgaag
      361 tccggcgctc tatccagctg agctacggac gcattcatac aaatttgtga aggggtgcct
      421 gatgggattt gaacccacga caaccagagc cacattctgg cgctctaccc ctgaactaca
      481 ggcaccaagt tacaaaaata ataataataa aataacgcgt ttctgtcaat accgcgcatt
      541 gattcaactt aaatcttagt gaaaaaaaaa tgttctcgta taaaaaattg ttttaagcct
      601 ctaaagtccc tttgaaaata ttccgatatt cttcatagag gtatactatg ggtaatgaca
      661 ttttaaatgc aaaacgatta tacgatgtag tgttggcaga aattgaactt atgtcacaaa
      721 ttatacaaat gcaaaaagca gtgcgtgagg caacaaagaa tcgtgattgg gagagtttac
      781 aaagcacttt ctactatata aatgaattat ctgaaggatt tttagagcta gaagaaagac
      841 gagtggctta tttcaaggat tttggtgcca aaacaggctc ggaattgcat caaattagcc
      901 aaaatttacc ctttcagttt aaaaacccta taacctctgt ttttactgaa ttgaaaaaaa
      961 aattactcga atccaaaata gaaaatgatg caataaacga atacatttct attacacaag
     1021 agtttattca aggtgttttt gacgaggttt tgcctcaacg aagaaatacg ctttattcta
     1081 aaacaggtac gctcataaag aatcaacctg aaagcattat tcttagtgca gttttataga
     1141 aggaaaaaaa gatgtctaca tttgcaggaa tagaactagg taagcgcagt ttaatggcac
     1201 atagtcagca aatcaatact gccggtcaca atatatcaaa cgcagaaacg gaaggctatt
     1261 cgcgacagcg tgttcatatt cgcgcttacg accccttgta tcggcctgac ttaaaccgcg
     1321 cagaaactcc cgggcaaatc gggcagggtt catctatcga gagtatcgct cgtgtacgag
     1381 atagtttgct cgatgcacgc atcacttcac aatcgcatct cgaaggttac tggtcaaaac
     1441 gcgaatccta ctatcaaatg ctcgaaagta tttataacga accagttgat gtatctattc
     1501 gttctacaat ggatgctttt tgggaagcct ggcaagaatt atcgctctat cctgaatcaa
     1561 aggctgcgcg tcaagcggta gtaacacgaa gcgagtcatt tatcgactct gtgaagcaac
     1621 gacacgacgg cttggtcggt gttgggacat tgcttaatgg cgatatcgaa gcaacggtgg
     1681 aacaggtaaa cagctacact cgagatatcg ctgcattgaa taaggaaata gttcgcagtc
     1741 gggcaatggg agacaaccca aacgacttac tcgatcgacg agaccttttg gtagacaaat
     1801 tatcaagcat tataaatatt accacagatg agcgcgaccc cgatgaattt atggtacacc
     1861 ttgacggaaa ggtcctcgtg caaggcaata tcgcacgaaa tttttctgtt gaagctataa
     1921 aagatggcac tgggtacaca aaggttatct gggcagatac aaaagaagac gcttttttca
     1981 acggcggttc tctcggaggc ttaatcgaat tgcgcgacgt cgatgttcga gaagagctac
     2041 aaagtttgaa caccttcgtt atgaactttg cagatttagt aaacgatatt caccgtaatg
     2101 cagttggggc aaataatacc actggtttag atttttttgt cgagcagccc tttgtgacca
     2161 acgccctcgg aaattatgac cgagatagcg acggtgtcga tgacagctcc tatatttttc
     2221 gtatgacagg tggcaacaca ttaaacccgc aagacaaggt gggtttcgac ggtacgattc
     2281 gactcgcttc tcgcactggg ctcgtggaaa taagctacta tgcaactgac acagtagaaa
     2341 ccataatcga taagataaat gattctgaaa gcgaggttaa ggcttacttg gatcgaaact
     2401 ctcatctcgt tctcaaggca acgactgcag caaatccaga aaatcccgac tttgttatac
     2461 gtcatgtgga agattcctca tacttcttga gccaatacac aggcttgtta gcagaaagtg
     2521 gggaagaggg cgcctatcga ttcgatcaag caaatgcggt aaacgctctt gtagaagata
     2581 aagctcgcta tgctgttgca cctgttttga acccaagtgc ctattttaca gtaaacgaag
     2641 caatcaaaaa agacgtgctc tctgttgcat ccgcctttcc tacaagctca ggacttgctg
     2701 aagttggaga cggttctgcc gcccttgcta ttgcctcatt acgtaacggt atgataatga
     2761 taggggaaaa caaaagcttt gacgattatt tttcagagac agttacaaaa atcggct
//
LOCUS       DAOSSW010000126         2816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_32335_length_2816_cov_8.525172, whole genome shotgun sequence.
ACCESSION   DAOSSW010000126 DAOSSW010000000
VERSION     DAOSSW010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32335_length_2816_cov_8.525172"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PKW26_08015"
     CDS             <1..630
                     /locus_tag="PKW26_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93542.1"
                     /translation="LDEIDLEEFIDEDSLRTEAETKQDTSFRNLDIFKEKLSLGDDTC
                     ENENGLVKGVDPLFTLDIKQPDFSALDSIKKEMSLEKSSPSETKIDQEEKEIVKDLSD
                     GAKPKLEELVLEQSSESDTAADAVDDYEELDSSDLVQYIDDNAAFSFAIQFKPPKKLE
                     SASLTNGSEKEHQESIVEHNGIFHVNKDIPFDTVEVDKDFVSLVDSVLE"
     gene            703..1110
                     /locus_tag="PKW26_08020"
     CDS             703..1110
                     /locus_tag="PKW26_08020"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOQ93543.1"
                     /translation="MSFKAYHRVRSYECDGYNHVNNAVYLNFLEHGRMDFLRHIGFDY
                     EKVVSLGYALYVTHIDIYYKASAFLYDELEISVVPKKLGKISGEFFQEIRKKDGTLCV
                     EAKVTWACVNSFGRPSKIPEDFLVPGLYPKEES"
     gene            complement(1122..1922)
                     /locus_tag="PKW26_08025"
     CDS             complement(1122..1922)
                     /locus_tag="PKW26_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOQ93544.1"
                     /translation="MLSNYHSHTDLCNHAKGYIGDYLLEAKSKKLRILGFSDHCPYPK
                     DGLDTWERVRMSIDESHRYIKDIRGVSKKENLEIYAGFECEWDFRYENWYRDFLLGEL
                     NADYLIFGPHWVYDTGNFIYAPEINTKKQLFTYFDTIIEGLASGLYAFLAHPDLIMSD
                     GRAWDANLKAGFTAIIEAAIQYNIPLEVNGQGFIKGKVKGEKGMRDPYPYDAFWDLVA
                     EKNIPVLCNSDAHFPENLVDSLHLARDYAKKMGLEIIEKLNFKNKKLL"
     gene            1996..>2816
                     /locus_tag="PKW26_08030"
     CDS             1996..>2816
                     /locus_tag="PKW26_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ93545.1"
                     /translation="MDIRLENLKKYYPSQNTLIKAVDDISIDIPSNTIYGIIGKSGAG
                     KSSLIRLISLLEKPDAGLVFYGGKEVSSLSEKELVLKRREVGMIFQNFNLFNSRNAFG
                     NVAYPLEINGKSKAFIEKRVKELLDLVGLSGREDAPMSTLSGGQKQRVAIARALANNP
                     GILFCDEATSALDPQTTKSILGLLRDIQKKIGLTIVMITHQMEVVRDVCEWVAVLDEG
                     QVVEKGLVKDIFARPKENITREFLSHMHYTEAEIKEQSEKTLLWSKDEAAFILRF"
BASE COUNT          902 a          467 c          559 g          888 t
ORIGIN      
        1 ctcgacgaaa tcgatttaga agaatttata gatgaagatt ctctaagaac agaggctgaa
       61 acaaagcagg atacatcttt tagaaaccta gatattttta aagaaaaatt atccctgggt
      121 gatgatacat gtgaaaatga aaatggttta gttaaagggg ttgatccact tttcacactc
      181 gatattaaac agcctgattt ttcagcctta gatagcataa aaaaagagat gtcccttgaa
      241 aaatcttccc ctagcgaaac taaaatagat caagaggaaa aagaaattgt aaaggatctc
      301 tccgatggtg caaaaccaaa gctggaagaa ttagttctag aacaatcaag tgaaagtgat
      361 actgctgccg acgctgtaga tgactatgag gagttggata gctcagatct agtacaatat
      421 atagacgaca atgctgcttt tagttttgct attcagttta aaccacccaa aaaacttgaa
      481 agtgctagtt taacaaatgg tagtgaaaag gagcaccaag aatcgattgt agagcacaat
      541 ggtatatttc acgtaaacaa agatattcct tttgatactg tcgaagtgga caaagatttt
      601 gtttcattag tcgattccgt actcgaataa aaaatctaga cttctttttc ttatttttat
      661 tttatgttag actagtgtaa taattcaaaa catggaggaa tgatgagttt taaagcctat
      721 catagagtac gttcgtatga atgcgatgga tataatcacg taaacaatgc tgtttaccta
      781 aattttttag aacacggacg catggatttt ttacgtcata ttggatttga ttacgaaaaa
      841 gtagtctccc tcggttatgc cctgtatgta acacatattg atatttatta caaagcttct
      901 gcatttttat acgatgagct agaaatttca gttgttccga aaaagctcgg taagatttct
      961 ggtgaatttt ttcaggaaat tcgaaaaaaa gacggcactc tttgcgttga ggcaaaggta
     1021 acctgggcct gtgtcaacag ctttggacgt ccatcgaaaa taccagaaga ctttcttgtg
     1081 cctggtttat atccaaaaga agaaagttaa atcgactctt tttatagaag ttttttattt
     1141 ttaaagttta atttttctat aatttctaaa cccatttttt ttgcatagtc ccgtgcaaga
     1201 tgtaagctgt caacaaggtt ttcaggaaaa tgtgcatctg aattgcaaag gacaggaatg
     1261 tttttttctg ctacaaggtc ccaaaacgca tcataggggt aggggtcgcg cattcctttt
     1321 tcacctttta cttttccctt tataaatccc tgcccattta cttcaagggg aatgttgtat
     1381 tgaatagcag cttcgataat cgcggtaaac ccagctttta ggtttgcatc ccaggctctt
     1441 ccatcggaca taatgaggtc aggatgtgcc aaaaaagcgt agagaccgct tgcaagaccc
     1501 tcgattattg tatcaaaata ggtaaaaagt tgtttttttg tgttaatttc tggagcatat
     1561 atgaaattgc ctgtatcgta gacccagtgc ggcccaaaga tgaggtaatc tgcattgagt
     1621 tctcctagca aaaaatctct ataccaattt tcatagcgaa aatcccattc acattcaaaa
     1681 ccggcatata tttcaagatt ttcttttttt gagacacctc gtatatcctt aatgtatcga
     1741 tgggattcat caattgacat gcgcactctc tcccaggtat caagtccatc ttttggataa
     1801 ggacagtggt cgctaaaacc gagtatccta agcttttttg attttgcttc taaaagataa
     1861 tcaccaatat atccttttgc gtgattacaa agatctgtgt gtgagtgata attactgagc
     1921 ataggtatat actagccttt attttaaatt acttatagtg gttgacaagt agttaaaata
     1981 atggaaaata aattcatgga catacgatta gaaaacttaa aaaaatacta cccttctcaa
     2041 aacaccttga ttaaagcggt tgatgatata agtatagata ttccctccaa taccatttat
     2101 gggattatag ggaaatctgg tgctggtaaa tcaagtttaa ttcgcttgat tagtttgcta
     2161 gaaaaacctg atgctggctt ggtgttttat ggtggcaaag aagtgagttc tctttctgaa
     2221 aaagaacttg tcctaaaacg tcgtgaagtg ggtatgattt ttcaaaattt taatcttttc
     2281 aattctcgaa atgcttttgg taatgtcgcc tatcccctcg aaataaatgg aaaatcaaaa
     2341 gcttttatcg aaaaacgagt aaaagaactt ttggaccttg tcggtttgtc gggaagggaa
     2401 gatgctccga tgagtacgct ttctggtgga caaaaacaac gggtggcaat tgcacgcgca
     2461 ttggcaaata atcccggtat tttattttgc gatgaggcga caagtgcctt agatccacaa
     2521 acgacaaagt ctattttggg gcttttacgt gatattcaaa agaaaattgg tttaacgata
     2581 gtgatgataa cgcatcaaat ggaagttgtt cgtgatgttt gcgaatgggt tgcggtgctt
     2641 gacgaggggc aggttgtcga aaaaggtttg gtaaaagata tttttgcccg tccaaaagaa
     2701 aatataactc gcgagttttt atcgcatatg cattatacag aggctgaaat aaaagagcaa
     2761 agtgaaaaaa cccttctgtg gtcaaaagat gaggcagctt ttattctacg ttttat
//
LOCUS       DAOSSW010000127         2736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_33603_length_2736_cov_3.975382, whole genome shotgun sequence.
ACCESSION   DAOSSW010000127 DAOSSW010000000
VERSION     DAOSSW010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33603_length_2736_cov_3.975382"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..631)
                     /locus_tag="PKW26_08035"
     CDS             complement(134..631)
                     /locus_tag="PKW26_08035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOQ93546.1"
                     /translation="MAIFIHESSYVDEGAVIGDGTKIWHFSHVLSGAQIGERCSLGQN
                     VNVGSRAIIGNGVKIQNNVSIYDDVIIEDDVFCGPSCVFTNVINPRAFVERKDEYKKT
                     VVKKGASIGANATIVCGVTIGEYALVGAGAVVTKDVPPYALVYGSPARVMGKVDRDGN
                     IVERV"
     gene            complement(648..1766)
                     /locus_tag="PKW26_08040"
     CDS             complement(648..1766)
                     /locus_tag="PKW26_08040"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOQ93547.1"
                     /translation="MKVPFYTSTREYEAHKEKFDSAVQNVMARGDFILGSEVKSFEQE
                     VASYAKSKYTIGVANGSDALVIASDVLGYKDNAEVLTPTFTFFASTSCIARLGGKPVF
                     TDVDPDTFCMDMNDAAHRITKNTVGILPVHLFLQMADMNSCMKLAKDNSLSVLEDAAE
                     AFGMKQLYNGEWKTAGTIGDFGVYSFFPTKTLGGYGDGGLITVQDEALFNHAKSLRTH
                     GSSIKYHHDYVGYNSRLDTLQAAILNVKLSLIDEGIAKREARANQYRSLLEGIGDLQL
                     PLVKEGNKPVYYVFTLKTEKRDALNEFLQKNGIGTSVYYPIPLHLQKCFSHLGYKKGD
                     FPVSEKLCETVISLPMYPELSEDEVTYICDKIKEFFAK"
     gene            complement(1822..>2736)
                     /locus_tag="PKW26_08045"
     CDS             complement(1822..>2736)
                     /locus_tag="PKW26_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOQ93548.1"
                     /translation="KDYKTMLEELQPDIVTIATESGKHKAIAIDCLNAGSHVICEKPM
                     ALSTSDAQEMIDCAKKNNKKLAVCFQNRFNAPIQKLREAYEKNRFGRVLHGMVQIRWN
                     RNEAYYAEAPWRGTWEQDGGTLMNQCTHGIDLLQWMMGEDAVRVQAQTRRFLRPIEAE
                     DFGCAIVEFSSGAVGIIEGTADVYPKNLNETLSIFGEKGTVVIGGLAVNKMETWRFSD
                     AESIGDTEDKVLNPNEKDPPTVYGFGHTALFADFIDAIEKDREPLVSGEKGKKALDII
                     LAIYESQKTGKAVDLPNNFSTMDMKNVF"
BASE COUNT          734 a          610 c          536 g          856 t
ORIGIN      
        1 aacaagggct agagcaaggt cgagcacaag aaaaaataaa aaccgctcgt agcatgaaat
       61 ctgaaggtct tcctcctgag caaatcaaac gttttactgg actttgcgaa ggtgaatatt
      121 agtaaaaaaa gtctcacacc ctttctacaa tatttccatc tctatccact ttgcccataa
      181 ctctggcagg actaccataa acaagggcat aaggcggaac atctttagtt accaccgctc
      241 ctgcaccgac aagtgcatat tcgccaatgg ttacaccgca gactatcgtt gcattagctc
      301 caatcgaagc tccttttttg acgactgttt ttttgtattc atctttgcgc tctacaaagg
      361 cgcgggggtt aataacattt gtaaatacgc aggagggacc gcaaaaaaca tcgtcttcta
      421 ttattacatc gtcataaata gaaacattgt tttgtatttt taccccattg ccaataattg
      481 ctcggcttcc cacatttacg ttttgtccca gagaacagcg ctcgccaatt tgtgcaccgc
      541 ttaacacatg agaaaagtgc catatctttg tgccatctcc tataactgcg ccttcgtcaa
      601 cataactaga ttcatgaata aatatcgcca taaatttatt cctttattta ctttgcaaaa
      661 aattctttta ttttatcaca gatataagtc acttcgtctt cgcttagttc tggatacatc
      721 gggagggata taacagtttc gcagagtttt tctgaaacag ggaagtcgcc ctttttatag
      781 cccagatggc taaagcattt ttgtagatgc agtggaatag gataatagac agaggtgcca
      841 atgccgtttt tttgtaaaaa ctcatttagt gcatctcgtt tttctgtttt tagtgtaaaa
      901 acatagtaca cgggcttatt cccctctttt acaagtggaa gttgtaggtc tccaatacct
      961 tctaaaagac ttcggtattg atttgcacgc gcttcccgct ttgctattcc ctcatctatg
     1021 agcgaaagtt ttacgtttaa tattgctgct tgcaaggtat cgagtcttga attatagcca
     1081 acataatcgt gatgatattt tatacttgag ccatgggtgc gtaaactttt agcatgatta
     1141 aaaagcgcct cgtcttgcac ggtaatgagc ccgccgtcgc catagccacc caaggtttta
     1201 gtgggaaaaa atgagtagac accaaagtcg ccaatggtac cagccgtttt ccactctcca
     1261 ttgtagagtt gcttcatgcc aaaagcttct gctgcatctt ctaaaacact tagagaattg
     1321 tcttttgcaa gtttcataca agagttcata tctgccattt gtaaaaaaag gtgaacaggt
     1381 aagatgccaa ccgtgttttt tgtaatgcga tgcgcagcat cgttcatgtc catgcaaaaa
     1441 gtgtcggggt caacatcggt aaatacgggc tttccaccaa gacgtgcaat acaagatgtg
     1501 gatgcaaaaa aggtaaaggt aggagttagg acctcggcat tgtccttgta gcctaagaca
     1561 tcgctagcaa taaccagggc atcgctgcca tttgcaaccc ctatggtata ttttgatttt
     1621 gcataagatg caacttcttg ttcaaaggac tttacttcgc ttcctagaat aaaatctcct
     1681 cgggccataa cattctgtac cgcagagtca aatttttctt tatgagcttc gtattctcga
     1741 gttgaagtgt aaaatggcac tttcataatt ttctcctaat agcaacaaga taaagtaagc
     1801 ttatgtcttt gcgagcaaaa tttaaaacac gtttttcatg tccatggtag aaaagttgtt
     1861 tggtaaatcc acagccttac ctgttttttg gctttcatag atggctagaa ttatatccag
     1921 cgcttttttc cctttttcgc cgcttacaag gggctcccta tccttttcta ttgcatcgat
     1981 aaagtcggca aagagggcgg tgtgaccaaa accataaacc gttggcggat ctttttcatt
     2041 tggattgaga accttgtctt cggtatctcc aatgctttct gcatcgctaa agcgccaggt
     2101 ctccattttg tttaccgcaa ggccacctat aacgacagtt cctttttctc caaagatgct
     2161 caaggtttcg tttaggtttt tgggatagac atccgctgtt ccttcgataa tgccaacggc
     2221 tccgcttgaa aactctacaa tagcacagcc aaaatcctct gcttcaattg gtcgtaaaaa
     2281 gcgccttgtt tgggcttgta cgcgaacggc atcttcgccc atcatccatt gcaaaaggtc
     2341 tatgccatgg gtgcattgat tcataagggt gccaccatct tgttcccagg tgccacgcca
     2401 gggggcctcg gcataatagg cttcgtttct attccatcga atttgcacca tgccatgcaa
     2461 tactctacca aaacgatttt tctcatatgc ctctcgcaat ttttgaatag gggcgttgaa
     2521 tcggttttga aagcagacag caagtttttt attgtttttc ttggcacagt ctatcatttc
     2581 ctgtgcatcg ctcgtagaaa gcgccatggg tttttcgcag attacatggc ttcctgcatt
     2641 gagacagtcg attgcaattg ccttatgctt accagattca gttgcaatgg taacgatatc
     2701 tggctgcaat tcttcgagca tagttttgta gtcttt
//
LOCUS       DAOSSW010000128         2706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_34136_length_2706_cov_4.993965, whole genome shotgun sequence.
ACCESSION   DAOSSW010000128 DAOSSW010000000
VERSION     DAOSSW010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34136_length_2706_cov_4.993965"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PKW26_08050"
     CDS             complement(<1..919)
                     /locus_tag="PKW26_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOQ93549.1"
                     /translation="MHITVNPLPKNLPLLMKSRYEEVPELYLQASKDSTGTFSYISYK
                     TVYENIICCALALKNLGIRKGSRVGLISDNRKEWLVADLALLSLGACDVPRGCDSMGS
                     EIRFILSFAECEFCFFENSRQLHKVLEKIDEVKTLKTAILFEDPSADIRAQAEKVGIQ
                     VLAFESLMEAGRKQGFEKRDSIEAIMDTIDTDDIATIIFTSGTTGTPKGVMLTHRNYI
                     AQCEVVHNVMNVEPGDFWLSVLPVWHSFERVIQYLAITFKSGLAYSKPIAAVMLPDFQ
                     AIRPQWMCGVPRLWEALAQGIYKNMRKEGG"
     gene            1067..2323
                     /gene="thiI"
                     /locus_tag="PKW26_08055"
     CDS             1067..2323
                     /gene="thiI"
                     /locus_tag="PKW26_08055"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOQ93550.1"
                     /translation="MYTESYLCQLGELTLKGGNLKTFEKRLVDNARSYLSPLDIKIQL
                     RAGRMYIDCRKEDSQAVEFCLSRLIGITGWAKTQVIDKNLDAIKEAVMREALFAKERG
                     AKTFKIETRRSDKSFPLNSYELSAQAASDVHSQAILQVNLHKPDLIIRIEVREKCFVY
                     GNAHKAPRGLPVGVSNKGLLLLSGGIDSPVAGYRMMRRGMKVESIYFHAYPYTSTEAQ
                     EKVEELARKLSLFGLETALNIIHFTEVQLKIRQQLKEIPTFISKLDNKEKKTEAYSTL
                     LLRFCMMKTASLLANYIHASCLISGESLGQVASQTIENLAITEAASPLPLLRPLIGLD
                     KEEIVETARYIDSYETSILPYEDCCVLFSPKHPVLKAHIEDVLFLYEALKVDALIQEA
                     FEKREIKIYRASDSIRKMLEDKNQKN"
     gene            complement(2320..>2706)
                     /locus_tag="PKW26_08060"
     CDS             complement(2320..>2706)
                     /locus_tag="PKW26_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ93551.1"
                     /translation="LTNFAKIYPKMDIRALGYINNMHEYIQASDCVVTKAGASTVMEI
                     LACQKPIIFSTFIHGQELGNVQFVVRNKLGWFLRSPKAIHEKIDQLFSDAEYRKSIQE
                     NLKSISIPSDNRVISRYIVEEKRALS"
BASE COUNT          862 a          562 c          503 g          779 t
ORIGIN      
        1 taccgccttc ttttcgcatg tttttgtaaa taccttgggc aagagcttcc cacaatcgag
       61 gtacaccaca catccactgt ggacgaattg cttgaaaatc tggaagcata acagcagcaa
      121 ttggtttaga ataagccaag ccacttttaa aagtgatagc aagatactga atcacgcgct
      181 caaaactatg ccaaacaggt aaaacagaaa gccaaaaatc gccaggctca acattcataa
      241 cattgtggac aacctcacac tgcgcaatat agtttctatg tgtaagcata acaccctttg
      301 gcgttcccgt tgtaccagaa gtaaaaataa tagtagcgat atcatctgta tcgattgtat
      361 ccatgattgc ttcaatacta tcgcgttttt caaaaccttg ctttcgtcca gcttccataa
      421 gactttcaaa tgcaagcact tggatgccga ccttttctgc ttgtgcacgg atatcagcac
      481 tcgggtcctc aaaaagaatt gctgttttga gtgttttcac ctcgtcaatt ttctctagaa
      541 ccttatgtaa ttgcctacta ttttcaaaaa agcaaaattc acattcagca aaagagagaa
      601 taaaacgtat ttcactgccc attgaatcac agccacgagg cacgtcgcaa gcaccgagag
      661 aaaggagcgc aaggtcagca acaagccatt ctttgcggtt gtcggaaata agccccacac
      721 ggcttccctt gcgtatgccc aagtttttta atgcgagagc acaacaaatt atgttctcat
      781 acactgtttt atagctaata tatgaaaaag tacccgtgct atctttagaa gcttgcaagt
      841 acagttcagg cacttcttcg taacgagact tcatcagaag tggtagattt tttggcaatg
      901 gatttacggt gatatgcatt attttctcct attaaatctt tgagatattt tatttatcaa
      961 atgatacttt acattatcac agattttgtc ataatgcaat actaggccta aatttttatc
     1021 tacaaagcca gtatacttta aaagcctcat tttgtatact aaaaatatgt atacagaaag
     1081 ctacctttgc caacttggag aactcacact taaaggtggg aacttaaaaa cctttgaaaa
     1141 acgacttgtt gataatgccc gatcctacct ttcaccttta gatataaaaa tacagcttcg
     1201 cgcaggaagg atgtacatag attgcagaaa agaagattct caggcggtag aattttgtct
     1261 ttccaggctt ataggaatta cgggctgggc aaaaacccag gttatagata aaaatctcga
     1321 tgctataaaa gaagctgtta tgagggaagc cctttttgca aaagaaaggg gtgcaaaaac
     1381 atttaaaata gaaacgaggc gaagcgataa atccttccct ctgaactcct atgagctttc
     1441 tgcccaagct gcaagtgatg tccacagcca agctattttg caggtgaatc ttcacaaacc
     1501 tgatctgatt attcgcatag aagtacgaga aaaatgcttt gtctacggca atgcacataa
     1561 ggctccgcga ggacttccag ttggcgtaag caacaaggga ctcttactct tatctggtgg
     1621 tatcgattct cctgttgcag gctatcgcat gatgaggcga ggtatgaagg tagagtctat
     1681 ttatttccat gcctatccct atacttcaac agaagcacag gaaaaagtag aagagcttgc
     1741 aagaaaattg agcctttttg gtttagaaac agccctcaat attattcatt ttactgaggt
     1801 acagctcaaa attcgccaac aattaaaaga aataccgact tttatatcga aactagacaa
     1861 taaagagaaa aaaacagagg cctacagcac gcttttattg cgtttttgta tgatgaaaac
     1921 agcaagcctt ttggcaaact atatccacgc atcatgtctt ataagcggag aaagtcttgg
     1981 acaggtggca agccaaacaa tagaaaacct tgctataaca gaggctgcaa gtccccttcc
     2041 gcttttgcgc ccccttatcg gactcgacaa ggaagaaatt gtagaaaccg cccgctatat
     2101 cgacagctat gaaacctcca ttctacccta cgaagattgt tgcgttcttt tttctcccaa
     2161 acatcccgtg ctcaaagccc atatcgaaga tgttcttttt ttatatgagg cattaaaagt
     2221 cgatgcgctt atacaagaag cttttgaaaa aagagaaata aagatatatc gtgcaagcga
     2281 tagtatacga aaaatgcttg aagataaaaa tcaaaaaaac taagaaagtg ctcttttttc
     2341 ttcgacgata tatctgctta tgaccctatt atcgcttggt atagaaatac tttttagatt
     2401 ttcttgaata ctttttcgat attctgcatc tgaaaaaagt tggtctatct tttcatgaat
     2461 tgcctttggg cttcgtaaaa accagccgag tttattgcgt acaacaaact gcacattccc
     2521 aagctcttgt ccatgtataa atgtagaaaa aataattggt ttttgacagg caaggatttc
     2581 catcaccgtc gaagccccag cctttgttac cacacagtcg gaggcttgaa tatattcgtg
     2641 catattgttg atataaccga gggctcgtat atccatcttc ggatagattt ttgcaaagtt
     2701 ggttaa
//
LOCUS       DAOSSW010000129         2609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_35858_length_2609_cov_4.699295, whole genome shotgun sequence.
ACCESSION   DAOSSW010000129 DAOSSW010000000
VERSION     DAOSSW010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35858_length_2609_cov_4.699295"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PKW26_08065"
     CDS             complement(<1..367)
                     /locus_tag="PKW26_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOQ93552.1"
                     /translation="MKNLKTIFAFLCMAIILTGAFAGGTKDTSKKSTDGRPTIRLITD
                     ATSIDDKSFNAAAWRGILEFYGDTWENQANRGKLYDVVTCQTMDMYVPNIKQASDEGY
                     DLICATGFTFADAIGEVAQL"
     gene            548..2362
                     /locus_tag="PKW26_08070"
     CDS             548..2362
                     /locus_tag="PKW26_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOQ93553.1"
                     /translation="MKDFLPISLEEARSQGIDEFDFILISGDAYVDHPSFGSALIGRL
                     LEGAGYKVGIIPQPDMSDPESIAVLGRPRLAFLLSSGNMDSMVAHYTANKKPRSQDFY
                     SPGGKAGFRPDRALFAYANAVRRKFKKVFLIGGGIEASLRRLSHWDYWQEKFRKSILL
                     DAKLDLIVYGMAEKALLEVAEVLAHGGGIEELKNVRGIVYAGSKKIDEIEAKERGLRL
                     SYLPSFDSFKEDKKLFAKSTALRMKENDFYSAGILVEPCDTRFVVQNPPSPPLSQEEM
                     DAVYDLPFTRKAHPVYEKSGGIPALKEVAFSITSSRGCFGSCSFCSLAFHTGRIIQSR
                     SKESLLKEAKILTEDPDFKGYIHDLGGPTANFYHKACEKQEAGKGACLGKECLWPSPC
                     SEINPSHTEYLAVLKAIRSLPKVKKVFIRSGIRFDYLLLEKDETFLQELCEHHLSGQL
                     KLAPEHVSPHVLEAMSKPAPEVYEEFKKRFTKMNKKLDKKQYIIPYFISAHPGSRLED
                     ALELALYLAKEGFVPDQVQDFYPTPGSLSTCMYATGIDPRTMEEIYIPNTVKERKLQR
                     ALLQFNKKENTALVREALKILGREDLVSVLRPYYGKKK"
BASE COUNT          755 a          529 c          588 g          737 t
ORIGIN      
        1 agagttgtgc aacttcacct attgcatcgg caaaggtaaa gcctgttgcg cagataaggt
       61 cgtagccttc gtctgaagct tgcttgatgt tgggaacata catgtccatt gtttgacagg
      121 ttactacgtc ataaagcttt cctcggtttg cttggttttc ccaagtgtct ccatagaact
      181 cgagtatgcc tcgccatgca gcggcgttaa aggacttgtc atcgatactt gttgcatcag
      241 ttataagacg aattgtaggg cgcccatctg tgcttttttt tgatgtgtct ttggttccac
      301 cagcaaatgc gcccgttaaa ataattgcca tgcatagaaa agcaaaaatt gtttttagat
      361 ttttcatttc tcctccttag gaaatctctt gtttctagtt taaattatag gtactttttg
      421 tttaaagtca atgaaaaaag acttgttaac tcctattatg gcagaatcct ggtctgttta
      481 gaacaagtaa aacaagcctt gcataagaca gacaagtatc agttttttgt gctatagtgt
      541 ttggagaatg aaagattttt tgcctatttc ccttgaagag gcgcgctctc aggggattga
      601 cgagtttgat tttatcctta tcagtggaga tgcctacgtt gaccatccgt cttttggctc
      661 agcgcttatt ggtcggcttt tggaaggagc gggctataag gtgggtatta ttccccagcc
      721 ggacatgtct gaccccgaat ccattgctgt tttagggcgc cctcgccttg cctttctctt
      781 atcgagtgga aatatggatt ccatggttgc ccattatacc gcaaataaaa aaccgcgctc
      841 tcaagatttt tatagccctg ggggaaaagc gggattccgt cccgaccgtg cactctttgc
      901 ctatgcaaat gctgttcgtc ggaagttcaa aaaagttttt ctcatcggtg gaggtataga
      961 agcatctttg agacgtttga gtcactggga ttattggcag gaaaagtttc ggaaaagcat
     1021 tctgcttgat gcaaagctcg atctcatcgt atacggcatg gcggaaaaag cgcttcttga
     1081 agttgcagaa gtgcttgcac atggcggtgg gattgaagag ctaaaaaatg tacgcggcat
     1141 tgtctatgca ggttcaaaaa aaattgatga aatagaagcg aaggagcgtg ggcttaggct
     1201 ttcgtatttg ccttcttttg attccttcaa agaagataaa aagctttttg caaaaagcac
     1261 cgcgctacgg atgaaggaaa atgattttta ttcggcaggc attcttgttg aaccatgcga
     1321 tacacgcttt gttgtgcaaa atcctccttc tcctcccctc agtcaagagg aaatggatgc
     1381 tgtgtacgac ttacccttta cgcgaaaggc gcatccagtg tatgaaaaaa gcggtggaat
     1441 tccagcccta aaagaagtgg catttagcat aacatcgagc cgtggttgtt ttggttcctg
     1501 tagtttttgc tcactcgcct ttcatacagg aagaataatt cagtcgcgaa gtaaagagtc
     1561 cttgcttaaa gaggcaaaaa tcttaacaga agaccccgac tttaagggct atatccacga
     1621 ccttggggga ccgacggcaa atttttatca caaggcctgt gaaaaacaag aggcggggaa
     1681 gggagcttgt cttggaaaag aatgcctttg gccaagccct tgtagcgaaa taaatccatc
     1741 gcatacagaa tatctcgccg tcctaaaggc aatacgctcc ttgccaaagg taaaaaaagt
     1801 ctttattcgc tctggtattc gctttgatta tcttcttttg gaaaaagacg agaccttttt
     1861 acaggaactt tgtgaacacc atctttcggg gcagctaaaa cttgctcccg aacatgtaag
     1921 cccccatgtc ttagaagcca tgtcaaaacc tgctcctgaa gtatacgagg aatttaaaaa
     1981 acggtttact aaaatgaata aaaaactgga taaaaaacaa tatattatcc cctattttat
     2041 atccgcacat ccgggaagcc gccttgaaga cgccctagaa cttgccctgt atttggcaaa
     2101 ggaaggcttt gttccagacc aagtccagga tttttatccg acaccgggat cactgtccac
     2161 atgcatgtat gcgacaggca ttgacccgcg gacgatggaa gagatttata ttcctaacac
     2221 agtaaaggag agaaaactac aacgcgcctt gctacaattt aataaaaaag aaaacacagc
     2281 cctggttcga gaagccctca aaatactcgg tcgtgaagac cttgtttctg tcttacgacc
     2341 ctattatgga aagaaaaagt agggagaggg acggagaaag cgaacttgac atacgcaaga
     2401 taatactgtc aagcgcaaga taatacttga cagcagaaat aagctgattt atacttttta
     2461 aatcaacgtt gccgaagagt tacgatagaa cacttgctcg aattgggaaa gtcctggttt
     2521 gagacaaatt atggtaagat aacttttttg acaataattt agaaggagat agagcacatg
     2581 gcatttatag acatggttga atggaaacc
//
LOCUS       DAOSSW010000130         2564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_36705_length_2564_cov_5.928657, whole genome shotgun sequence.
ACCESSION   DAOSSW010000130 DAOSSW010000000
VERSION     DAOSSW010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36705_length_2564_cov_5.928657"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PKW26_08075"
     CDS             <1..294
                     /locus_tag="PKW26_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOQ93554.1"
                     /translation="LDDFGTGYSSLSYLKELPLTTLKIDKAFIENITTPNSLEAEITD
                     SIISLVSKMGLDTIAEGVESEEQFEALKKINCRTSQGYLMGRPMPESECESLL"
     gene            complement(340..1227)
                     /locus_tag="PKW26_08080"
     CDS             complement(340..1227)
                     /locus_tag="PKW26_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOQ93555.1"
                     /translation="MKYTLPFTIPSTDMDDHLRFTTLAMITAVQEVTSVHYASGGLSI
                     PHLQRQGITWVLVKQRFEISEYPLWMDEVLLSTWAKQAKGPFFLRDFSFSFNKNGKKA
                     TLDQAFRDFQVKKEGFCKEEGSISVVEKEAESQKPFLKGTSAWMVLDAKSLKPIRPSE
                     NVMGTLEMGTEDALPSQFSKIPLAEDLGKKWDCVQEIEPTVLDIDVNDHVNNLSYIRW
                     ILSYLPKTFYKGMLIESIDTYFMSSALFNQRLLVRSSECEDGKLLHSIIRKDDGTDVF
                     RAESRWKKAELLSRQACIE"
     gene            1283..2506
                     /locus_tag="PKW26_08085"
     CDS             1283..2506
                     /locus_tag="PKW26_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOQ93556.1"
                     /translation="MQKYYKYILILASFLCISHGYSEELRFKYAKGDSYRILSTVKQN
                     VIVNGKYDHSAEIVNRISVEIIDVDEDGWGEHQARFMTSETAVKGLNEKEFTWGQEYE
                     SLFWISPLGEYSVSDEYYMPTTRNVPLFPTRNLNPNEGWEGEGLEVHDLREVFGIEKP
                     YIIPFKARYIYKGEIEIDGKKLQEIAVFYTLDYANPVPKKEEYSKNDQIIDFPFHTSG
                     NFEQTIYFDAELGAMHSYKENFKILIKTALGNTYEFSGFAESEINELKKVPQKTVEDA
                     IKNLDLENTSVVQDERGLTITIENIQFLADSAILLAKEKEKLIKLAEILKDFPDNDLL
                     ISGHTALAGSEAARLKLSKERAQAVANFLVSIGLKDAYHIFTQGFGAEKPIAPNETEA
                     GKAKNRRVEITILEN"
BASE COUNT          872 a          557 c          455 g          680 t
ORIGIN      
        1 ctcgatgact ttggcacagg ctattcttcc ctcagttatc taaaagaact tcccctcact
       61 accttaaaaa tagacaaagc ctttattgaa aatataacaa ctcctaactc acttgaagct
      121 gaaataacag actcaattat ctcccttgtt tcaaaaatgg gtctcgacac catagctgaa
      181 ggagttgaaa gcgaagaaca attcgaagca ctgaaaaaaa taaattgccg cacaagtcag
      241 ggctatctta tgggacgccc catgcccgaa agcgaatgtg aaagcttact ctaaagaata
      301 aatctacaag ctctatcgat tatcttttga tctatacttt tattcaatgc aggcttgacg
      361 agaaagtagc tctgcttttt tccaacgact ctcagcacga aagacatctg tcccgtcatc
      421 ttttcgaata attgaatgca aaagtttccc atcttcacac tcagaagagc gcacaagcag
      481 cctttgatta aataaagcac tggacataaa ataggtatcg atgctttcga tgagcatccc
      541 cttataaaat gttttcggca aataggacaa tatccaacga atatacgaaa gattatttac
      601 atggtcattt acatcgatat ctagcacagt cggctcaatc tcttgtacac aatcccattt
      661 ttttccgaga tcctcagcaa gggggatttt tgaaaactgc gagggaagcg catcctctgt
      721 acccatctcc aatgttccca ttacattttc actcgggcga ataggtttta agctttttgc
      781 atcgagcacc atccaggctg aagttccctt taaaaaaggt ttttgggact cagcctcttt
      841 ttctaccaca gaaatcgaac cctcttcttt gcaaaatccc tcttttttta cttggaagtc
      901 tctaaaagct tgatccaaag ttgctttttt cccgttttta ttaaatgaaa aagaaaaatc
      961 tcgtaaaaag aagggaccct ttgcttgctt tgcccaggta gaaagcaaaa cctcatccat
     1021 ccataaggga tattctgaaa tttcaaatct ttgtttcacc aaaacccagg ttattccctg
     1081 cctttgtaaa tgcgggatgg aaagcccccc tgaggcataa tgcaccgagg ttacctcttg
     1141 cacagctgtt atcatagcaa gcgtagtaaa tctaaggtga tcgtccatat cggtcgatgg
     1201 tatagtaaaa ggcaatgtgt atttcatctt taaagaatag cattctccga ataaatacac
     1261 aagctaaaac aaaggaaata taatgcaaaa atattataaa tacatactca tcctcgccag
     1321 ttttctctgt atctctcatg gatattcaga agaattacgt tttaagtatg caaagggcga
     1381 cagctataga attttatcca cagtaaaaca aaacgtcatc gtaaatggca aatacgacca
     1441 cagcgccgaa atagtcaatc gcatatcggt cgaaataata gacgtcgacg aagatggctg
     1501 gggagaacat caagctcgtt ttatgacaag tgaaacagcg gtaaaagggc ttaacgaaaa
     1561 agaatttacc tggggacaag aatacgaaag cttgttttgg atttcacccc ttggcgaata
     1621 ttcggtgtca gatgaatatt atatgccaac cacaagaaat gtaccacttt tccctactcg
     1681 aaaccttaac ccaaatgaag gatgggaagg cgaaggttta gaagtacacg atttacgtga
     1741 agttttcggc atagaaaaac cctatatcat cccttttaaa gcaagatata tttataaagg
     1801 tgagatagaa attgatggaa aaaaactaca ggaaatagcg gttttttaca cactcgatta
     1861 tgcaaatccc gtcccaaaaa aagaagaata tagcaaaaac gaccagatta ttgattttcc
     1921 ctttcacaca agtggcaact ttgaacaaac gatttatttt gatgcagaac tgggcgctat
     1981 gcactcctac aaggaaaact tcaaaatact cattaaaacc gccttgggaa atacctacga
     2041 attttcaggc tttgctgaat ctgagataaa cgagctaaaa aaagtacccc aaaaaaccgt
     2101 cgaagatgct atcaaaaatc tcgatttaga aaatacatca gtcgtacaag atgaacgcgg
     2161 cctcactatc actatagaaa acattcaatt ccttgcagat tccgcaatcc ttcttgcaaa
     2221 agaaaaagaa aaactgataa aacttgcaga aatcctcaag gattttcccg acaacgactt
     2281 gcttatctca gggcacactg ccctcgcagg aagcgaagca gcacgcctta aactatcgaa
     2341 agaaagagcg caagccgtcg caaactttct cgtctcgatt ggactcaaag acgcctatca
     2401 tatttttacc cagggctttg gcgcagaaaa acccatcgct ccaaatgaaa cagaagcagg
     2461 aaaggcaaaa aaccgccgcg tagaaattac aatcttggaa aattaagtgc atagattacg
     2521 ttttttctaa catccgtgtt agaaaaaact tcgacagttt cgga
//
LOCUS       DAOSSW010000131         2559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_36797_length_2559_cov_6.055112, whole genome shotgun sequence.
ACCESSION   DAOSSW010000131 DAOSSW010000000
VERSION     DAOSSW010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36797_length_2559_cov_6.055112"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..515)
                     /locus_tag="PKW26_08090"
     CDS             complement(99..515)
                     /locus_tag="PKW26_08090"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOQ93557.1"
                     /translation="MKDRDTADLEKLIKEAIEARKKAYVPYSKFLVGAALETKDGKIY
                     HGCNIENASYSLTNCAERTAFFAAIADGYTRGDFKRLAVVGQTEGPISPCGACRQVID
                     ELGGPDLEIILTNLDGVVEVTEIKKLLPGAFSDQDL"
     gene            complement(569..1900)
                     /gene="deoA"
                     /locus_tag="PKW26_08095"
     CDS             complement(569..1900)
                     /gene="deoA"
                     /locus_tag="PKW26_08095"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOQ93558.1"
                     /translation="MYLPQETIRRKRDKEVLTQDEIQSFIRGVTDNSISEAQISALTM
                     AIFLNGLSKEECVHLTLAMRDSGDIMQWKHLDLPGPIIDKHSTGGVGDTVSLMLGPLF
                     AACGGFNPMISGRGLGHTGGTLDKLASIPGYNPFPTNEQLTEAVKIAGTAIIGQTGNL
                     APADKRIYAARDVTATVESIPLITASILSKKLAGGLDYLVMDVKTGSGAFMPTPALSE
                     ELARSIVSVGSGAGLGLSALLTDMNQSLAPAAGNAVEVQCAIDYLTGKAKPSRLHEVT
                     IELCAEGLLASGLAKDLDDARKKLNASLDSGLAAEKFAKMVHILGGPSDILENPKKYL
                     AQAPVKIPVKAEKNGYISRFDTRDIGLAVVALGGGRLRSDQVIDPAVGLTDIVELGQE
                     VVQGEDLMMIHAQSEKDAEIALKMIQKACYIHDEKPRALPLIPKRISVQDL"
     gene            complement(1915..2559)
                     /gene="deoC"
                     /locus_tag="PKW26_08100"
     CDS             complement(1915..2559)
                     /gene="deoC"
                     /locus_tag="PKW26_08100"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOQ93559.1"
                     /translation="MELNNFIDHTILKATATKEDILQLCEEAKKHQFFSVCVNSSWVS
                     LAKEVLSGSDVKICAVVGFPLGAMSTEAKIFEAERAILAGADEIDMVINIGYLLSGMN
                     EVVKEEIATIKKTIGNKILKVIIETCYLTDDEKKLASELAVLARADFVKTSTGFGTGG
                     ASLEDTALMLETVKDKAYVKASGGVRDREAAEAYIKQGVMRLGTSNGIAIIEAE"
BASE COUNT          717 a          584 c          454 g          804 t
ORIGIN      
        1 ctttcaagag atttatttga aagttgtttg cgaacaggct atcttacaaa atcaacagtt
       61 tctcaaaaaa aagctatcac cgcctagaac ttctattttt acaaatcttg atcggaaaaa
      121 gctcctggca ataatttttt tatctctgtt acttctacta caccatctag gtttgttaga
      181 attatttcta aatcaggacc gcccaattca tctataacct gtcggcatgc tccgcaggga
      241 gatatgggac cctctgtttg acccacgaca gcaagacgtt taaaatcacc tcgggtatag
      301 ccgtcagcaa tcgcagcaaa aaaagcagtt cgttctgcgc agttcgttaa actgtaggat
      361 gcattttcta tattgcatcc atggtagatt tttccgtcct ttgtctccaa agctgcaccg
      421 acgagaaact tagagtacgg tacataggct ttttttcgtg cctctatagc ttcttttatc
      481 aatttttcta agtcagcagt atctctatct ttcattttac caccattcct tttatttgtt
      541 tccaaacttg ccaagtaaaa aaacacttct ataggtcttg aacgctaata cgctttggta
      601 taagcggtaa agccctaggt ttttcgtcgt gtatataaca ggccttttgt atcattttga
      661 gagcaatttc tgcatctttt tcactttgcg catgaatcat cattaaatct tcgccttgaa
      721 caacttcttg cccaagctca actatatctg ttaaaccaac tgctggatct ataacttgat
      781 cgcttcgtaa gcgtccacca cccagtgcaa caacagcaag accaatatct cgcgtatcaa
      841 atcgagagat atagccattt ttttctgctt ttacaggaat tttcacaggc gcctgtgcca
      901 aatatttttt aggattttcc aaaatatcgc ttgggccacc gaggatatgc accattttag
      961 caaatttttc tgctgcaaga cctgagtcaa ggcttgcatt gagttttttt cgagcatcat
     1021 ctaaatcctt tgcaagccct gaagcaagta agccttctgc acaaagctcg atagtaacct
     1081 cgtggaggcg agatggcttt gccttccctg taaggtaatc gatggcacat tgtacttcaa
     1141 ccgcatttcc tgccgcgggc gcaagcgact gattcatatc ggttaaaaga gcagaaagcc
     1201 ctaaaccagc tcccgaaccg accgaaacga tggaacgtgc gagttcttca gatagtgcag
     1261 gtgtcggcat aaaggcgcca gaaccggttt ttacgtccat gacaagataa tcaagaccac
     1321 cagcgagttt ttttgagagg atggatgcgg taataagtgg aatggactca accgttgcgg
     1381 ttacatctcg cgccgcataa atacgtttgt cagctggagc gagatttcct gtttgcccta
     1441 taattgctgt tcctgcaatt tttacagctt ccgtcaattg ttcatttgta ggaaagggat
     1501 tataaccagg aatagatgca agtttatcga gtgttccacc ggtatgcccg agacctctgc
     1561 ctgaaatcat gggattaaaa ccaccacagg cagcaaagag gggaccgagc atcagagaaa
     1621 cagtgtcccc tactccccca gtagagtgct tatctataat tggaccaggt aagtctaaat
     1681 gcttccattg cataatatca ccagagtcgc gcatagcaag tgttaaatga acacactctt
     1741 ctttgctcaa accatttaaa aaaattgcca tagttaaagc cgaaatttgt gcttctgaaa
     1801 tagagttgtc agttacaccg cgaataaaag attgtatctc atcttgagtc aatacttctt
     1861 tgtctcgttt tcttcgaatt gtttcttgtg gtaaatacat atatcctcca aattttactc
     1921 agcttcgatt attgcaattc cattgcttgt tcccaatctc ataacaccct gttttatata
     1981 agcttcagca gcctcgcgat ccctcactcc tccagaggcc tttacatagg ctttatcttt
     2041 gactgtttct agcatgagag cggtatcttc taaacttgca ccaccagttc caaaacctgt
     2101 ggaagttttt acaaaatcag ctcgtgctaa aacagcaagc tcagatgcca gttttttctc
     2161 atcgtctgtt agataacaag tctctattat tacttttaaa attttattgc caattgtttt
     2221 ttttatggta gcaatttctt cttttactac ttcattcata ccagacaata aataaccgat
     2281 atttatgacc atgtctattt catcagcacc tgcaagaata gctctttctg cttcaaaaat
     2341 tttggcctct gtagacatag caccgagtgg aaagccaacg acagcacaga tcttcacatc
     2401 agatccgctt aagacttctt tcgctaatga aacccaagac gaatttacac aaacagaaaa
     2461 aaactggtgt ttctttgcct cttcacatag ttgtaaaata tcttcctttg tcgcagtagc
     2521 ttttaatatt gtatgatcga taaaattatt taattccat
//
LOCUS       DAOSSW010000132         2287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_42956_length_2287_cov_3.991487, whole genome shotgun sequence.
ACCESSION   DAOSSW010000132 DAOSSW010000000
VERSION     DAOSSW010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42956_length_2287_cov_3.991487"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..880
                     /locus_tag="PKW26_08105"
     CDS             125..880
                     /locus_tag="PKW26_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOQ93560.1"
                     /translation="MDTHSFPSINKNRKIGSLIKTSTVDFPGCISAALFLHGCNLRCP
                     YCYNIDLVTGKLDEEEAFSFNEVIAHLEKRKKVLSGFVISGGEPSISPYLPHLLKVAK
                     EIGYKTKLDTNGMKPSYLESILANPKISPDYIALDIKTKPEKYHLLGGAHAPMGEESA
                     GEKIQRSIEILASLPSDKREFRTVLVPGLVNAEDVKEIAHLLPKDASWFFAEFRNDSC
                     IDPAYNNIEAYEKKELDAIIHQAQLIIPKAALR"
     gene            complement(877..>2287)
                     /locus_tag="PKW26_08110"
     CDS             complement(877..>2287)
                     /locus_tag="PKW26_08110"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93561.1"
                     /translation="YCGLEYTISLEPGEEKSYGFFAGIGLDTLETIKAKYKSASDVKD
                     EMLSIRKDWSEKLSLDIPSIKNEDFSAYLNSFFGYQIYQQSAGMVRGTFRGFRDVAQD
                     AMAFVHYEPKKTRELLIDMCTRQYKDGRCLRQWNTEGGANDERDFRDLPYWTLLALHT
                     YEKVTGDASIYSAKAKWQDSETEDTLWEHALQGVRYATKIGSHDLIKLGIGDWNDALN
                     GPGPEGGSVFLNQIAYLALTYIEEIGAKHNLDTKVFADMSSSEMKEKLYAGVMKYWNG
                     SWFGRALSEDGKILGTTKDFKTADGFVSDGTERLFLLSQAWFTMSGMAERSDEDEAIG
                     QKALDNMLRYLEIEEGLLKMKPGYAEYEAITGNISGLAPGMAENFAIYNHAASFAVYA
                     LFQAGRNEDALRIMKKILSFTKDWKKTKAEPYVLVNFYNGGFYKEKAGEGGISWLTGT
                     INWLSMSLYDYMIPRGLDL"
BASE COUNT          681 a          521 c          399 g          686 t
ORIGIN      
        1 attcttgagc cagctcttac tgcagcttct tagtctgtat actaaaaaaa cattggcagt
       61 ttatttggtg gatttatcgc ttgaatcgac tgtcaatgtt ttatgtttag aaaataaaaa
      121 tagtatggat acacatagct ttccttcaat caataaaaac cgaaaaatag gctccctcat
      181 taaaacaagt actgtagatt ttcccgggtg catttctgca gccttatttt tacacggctg
      241 caatttacgc tgcccctatt gttacaatat agaccttgta actggaaaac ttgatgaaga
      301 agaagccttt agcttcaatg aagtgattgc ccatttagaa aaaagaaaaa aggtactctc
      361 gggctttgtt atttcaggcg gagaaccgag catttctccc taccttcccc atctattgaa
      421 agtggcaaag gaaataggct ataaaacaaa gcttgacacc aatggcatga aaccgagcta
      481 ccttgaatcg atacttgcca acccgaagat ttcgccagac tatattgccc tcgatattaa
      541 aacaaaacct gaaaaatatc acttactcgg tggcgcccat gctcctatgg gagaagaaag
      601 tgctggagaa aaaatacaac gctctataga gattctcgcc tctcttccct cagacaaaag
      661 agaatttaga actgttcttg ttccaggtct tgtaaacgca gaagatgtca aagaaatagc
      721 ccatctttta cccaaagacg cttcgtggtt ttttgcggag tttcgaaatg attcatgtat
      781 agaccccgca tataacaata tagaagccta tgaaaaaaaa gagcttgatg caataattca
      841 ccaagctcaa ttaatcatac caaaggctgc tcttcgttaa agatcaagcc ctcgtggaat
      901 catatagtca tacaagctca tagataacca gttaatagtt cccgttagcc aagaaatacc
      961 accttctcct gccttttctt tgtaaaatcc accattataa aaatttacga gaacataggg
     1021 ctcagccttt gtctttttcc aatcttttgt aaagcttaga atttttttca taatgcgtaa
     1081 agcgtcctca tttcttcctg cttggaaaag tgcatagaca gcaaaagagg ctgcgtgatt
     1141 atatattgca aagttttcag ccataccagg tgcaagccca gaaatatttc ctgtgattgc
     1201 ttcgtattca gcatagccag gtttcatttt taacaagcct tcttctattt caagatagcg
     1261 caacatattg tcgagggctt tttgtcctat cgcctcatcc tcatcacttc gctcagccat
     1321 accgctcatg gtaaaccaag cttggcttaa aagaaagagg cgctctgttc cgtcgcttac
     1381 aaaaccgtca gctgttttaa aatcttttgt tgtaccgagg atttttccgt cttcacttaa
     1441 cgcgcgacca aaccaagaac cattccaata cttcataact cctgcataga gcttttcttt
     1501 catttcagaa ctactcatat ctgcaaagac tttggtatct aaattatgct ttgctccaat
     1561 ctcttcaatg taggtgagag caaggtaggc aatttgattt aagaaaactg agcctccctc
     1621 aggaccagga ccgtttaaag catcattcca atcaccaatg ccaagcttta ttaaatcatg
     1681 gctgccgatc tttgttgcat aacgaacacc ttgcaaggca tgttcccaaa gcgtatcttc
     1741 tgtttcgcta tcctgccatt ttgcctttgc gctgtagata cttgcatcac ccgtaacttt
     1801 ctcataagta tgcaaagcta acaaggtcca atacggcaag tctctaaagt ctctttcgtc
     1861 gttcgctcca ccttccgtat tccattggcg taaacagcga ccatctttgt actgcctcgt
     1921 acacatgtcg attaaaagtt ctcttgtttt tttaggctca tagtgcacaa aggccattgc
     1981 atcttgggct acatcgcgaa aaccacggaa ggttccgcga accatgcctg ctgactgctg
     2041 ataaatttga tagccaaaaa aactatttaa gtaggcagaa aaatcctcat tttttataga
     2101 aggaatatct aaggaaagtt tttcagacca gtcttttcga atgctaagca tttcgtcttt
     2161 aacatcagaa gcagacttgt attttgcctt tattgtttca agggtatcta gaccaatccc
     2221 cgcaaaaaaa ccataggatt tttcttcacc tggctccaag ctgatagtgt actcaaggcc
     2281 gcaatat
//
LOCUS       DAOSSW010000133         2210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_45003_length_2210_cov_6.101160, whole genome shotgun sequence.
ACCESSION   DAOSSW010000133 DAOSSW010000000
VERSION     DAOSSW010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45003_length_2210_cov_6.101160"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PKW26_08115"
     CDS             complement(<1..905)
                     /locus_tag="PKW26_08115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOQ93562.1"
                     /translation="MEKKRASRNKTLKLSDEERSLYKKNLIYEIKNAHIDSLINKTIC
                     SDLFSVIDALPAASVDLLILDPPYNLAKNFHGFRFSQKNNEAYLDYLRSWFPKLMHCL
                     KATASIYLCGDWKNTYCLYRIMSEYTIVRNRIIWQREKGRGAKQNWKNATEDIWFGTV
                     GNEYYFDVEAVKQKRRVLAPYRKDGKPKDWEETEDGNFRLSFPSNFWDDISIPYWSMK
                     ENTDHPTQKPEKLIAKLILASCPVNGIVLDPFLGSGTTSVVAKKLGRNYIGIEMNEEY
                     CLWAEKRLEQAETEKAIQGYTDGVF"
     gene            complement(980..1051)
                     /locus_tag="PKW26_08120"
     tRNA            complement(980..1051)
                     /locus_tag="PKW26_08120"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1016..1018),aa:Glu,seq:ctc)
     gene            complement(1056..1126)
                     /locus_tag="PKW26_08125"
     tRNA            complement(1056..1126)
                     /locus_tag="PKW26_08125"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1092..1094),aa:Gln,seq:ctg)
     gene            complement(1166..2077)
                     /locus_tag="PKW26_08130"
     CDS             complement(1166..2077)
                     /locus_tag="PKW26_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOQ93563.1"
                     /translation="MADIKKAHLIGLGAVGATYASLLHKAEGSPIEIIVDEERAARYR
                     EGILINGENVHFNLALQTEAREKAELILIAVKAHHLEATIEAIRPFVGSETLILPLLN
                     GITSEEILAKAFGSEKILHGFCVALDSTKVNNEIRFTQTGKIVFGTAFGLEKEADSVE
                     AFFIKTKLPHTKADDILREKWWKFMMNVGINQISSILGANYGVFQKQAKARELMKDAC
                     LEVLKIAEAKQIQLSEADIDGFFPIIDGLSPEGKTSMLQDIEAGRKTEVELFSLAVIE
                     IGKKLGIATPINTLLYKMIRVLEEKNS"
BASE COUNT          587 a          443 c          440 g          740 t
ORIGIN      
        1 caaaatactc catcagtgta gccctgaatt gctttttcgg tttctgcttg ttcaagacgt
       61 ttttctgccc aaagacaata ttcttcgttc atttcgatgc ctatatagtt tctgcccagt
      121 ttttttgcaa caacggaggt cgtacctgaa cctaaaaaag gatcgagaac aatgccgttg
      181 acgggacaac ttgcaagaat gagcttggca ataagttttt caggtttttg cgtgggatgg
      241 tcggtatttt ccttcatcga ccaatagggt atggaaatat catcccaaaa attgcttgga
      301 aagcttaggc gaaaattgcc gtcttcagtt tcttcccaat cttttggctt tccgtccttt
      361 ctatatggcg ctaaaacacg ccttttttgt ttcactgctt ccacatcgaa ataatactca
      421 ttgccgacag tgccaaacca aatatcttcg gtggcatttt tccagttttg ctttgcgcct
      481 cgcccttttt ctctttgcca gataattcta tttcgaacaa tcgtgtattc gctcattatg
      541 cggtagaggc agtaggtatt tttccaatcg ccacaaagat agatagatgc agtggctttg
      601 aggcagtgca tgagttttgg aaaccaagag cgaagatagt ccaagtatgc ctcattattt
      661 ttttgtgaaa agcgaaagcc atgaaagttt ttagctaaat tatagggtgg gtcgagaata
      721 agaagatcga cagaggctgc tggaagcgca tcgattacag aaaaaaggtc agaacatatt
      781 gttttattta tgagagaatc gatatgagcg ttttttattt cgtagatgag gtttttttta
      841 tataaactgc gttcttcgtc gcttagtttt aaggttttat ttcggctcgc tcgttttttt
      901 tccatagata tattgaagta tagcaaggat atttttcttt gtcatctata agaatgagaa
      961 aatgagagtt tgctttttgc agtccctagg ggagtcgaac ccctctttaa agaatgagaa
     1021 tcttttgtcc taaccgatag acgaagggac cttgtttccc cggggaggat ttgaaccccc
     1081 acaagcagaa ccagaatctg cggtgctacc attacacaac cggggatggt attctcttaa
     1141 ctatagcaaa aaattaagat tttcgtcaag aatttttttc ttccaaaacg cgtatcattt
     1201 tatagagcag ggtatttatc ggtgttgcta tccctagttt ttttccaatt tcaataacag
     1261 caagtgaaaa aagctcaact tcggtttttc ttcctgcttc aatatcttgc agcatcgaag
     1321 tttttccctc gggagaaaga ccgtcgataa tgggaaaaaa tccgtcaata tctgcttcgc
     1381 tcagctgaat ctgtttcgcc tctgctattt ttaaaacttc aagacaggca tctttcataa
     1441 gttctcttgc ctttgcttgt ttttgaaaga cgccgtagtt tgctcccaag atgctcgata
     1501 tctggtttat tccgacattc atcataaact tccaccattt ttctctgaga atatcatctg
     1561 cctttgtatg tggaagcttt gttttaataa aaaatgcttc aacagaatcg gcttcttttt
     1621 ctaagccaaa ggctgttcca aaaacaattt ttcctgtttg ggtgaaacgt atttcattgt
     1681 tgaccttggt tgaatcaagg gcgacacaaa acccatggag gattttttca cttccaaagg
     1741 cctttgcaag gatttcttca ctcgtaatgc cgttcaataa gggaaggatg agtgtttcac
     1801 ttccaacaaa ggggcgaata gcttctatag tagcctctaa gtgatgtgcc tttaccgcga
     1861 tgagtatgag ctcagctttt tctcttgcct ctgtttgaag ggcaagatta aaatggacat
     1921 tttcgccatt tatgagaatg ccttccctat atcgagcagc tcgctcctcg tcgacgatta
     1981 tttcaatggg gcttccttcc gccttgtgta aaagagaagc ataggttgca ccgactgcac
     2041 caagtcctat gagatgtgct ttttttatat ctgccattat tattcccttg tttttttcta
     2101 gtataggctt aaaagacaga atgtgcaaga aagccttccc attgacagag aaggtttttt
     2161 tcttcaaact tcttatatgc aaggaaaaat aattgcgggt tcaaacaatg
//
LOCUS       DAOSSW010000134         2153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_46666_length_2153_cov_4.611535, whole genome shotgun sequence.
ACCESSION   DAOSSW010000134 DAOSSW010000000
VERSION     DAOSSW010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46666_length_2153_cov_4.611535"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..481)
                     /locus_tag="PKW26_08135"
     CDS             complement(44..481)
                     /locus_tag="PKW26_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOQ93564.1"
                     /translation="MKTLLSLSYPWIWLIIMIVLVAIEAFTFSLTTIWFALGALLMIL
                     ISLLGLSFPVQLLIWLIISSVLLYFTRPYALGKLKVGKVATNVDSLVGKSALVVKTIT
                     KHDKGEIKINGIIWSAKTEDDAELAEGSECEIVAIQGATAIVT"
     gene            620..1324
                     /gene="deoD"
                     /locus_tag="PKW26_08140"
     CDS             620..1324
                     /gene="deoD"
                     /locus_tag="PKW26_08140"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOQ93565.1"
                     /translation="MSIHIEAKKGDIADIVLLPGDPLRAEFIAEHFLENAVCYNRVRN
                     MLGFTGTYNGVRVSVQGTGMGQPSISIYANELFDFYGVQTAVRIGTAGAVHTSTKVRD
                     IVIAPSACSDSSILTQRFGSLHFAPTADFSLVYKTYETAKKLGIPVLTGPIASSDLFY
                     DVNDNWKKWAEYGVLAIEMEAAELYTLAAKYGRKALAIVTISDHIATGETTTSEERQT
                     TFKDMMKLALEATTSL"
     gene            1460..>2153
                     /locus_tag="PKW26_08145"
     CDS             1460..>2153
                     /locus_tag="PKW26_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93566.1"
                     /translation="MKPTLVVLAAGMGSRYGGVKQIDSVGLNGETLLDFASYDALQAG
                     FGKIVYIIRKDIEKDFRERFFDRVAKNANVDYVFQDRDTLFSDEEKKLITNRQKPWGT
                     IHALLCAEKAVNENYAVINADDYYGKSAFVTLGKHLSTVDPSSDEHAMVGYILGNTLS
                     KSGTVSRGVCELKDSYLMGMRENIKIGYEGDGITGELDGEKKVYDGKEIVSMNFFGFT
                     PKSFAYFNEFFDD"
BASE COUNT          691 a          352 c          455 g          655 t
ORIGIN      
        1 aaaggcaatt aaagcaatta aaatatacat atttcctcct tatttatgta acgatagcgg
       61 ttgcaccttg tatggcaaca atctcgcatt cacttccttc agctaactca gcatcatctt
      121 ctgtttttgc gctccaaatg ataccattta tttttatctc acctttatcg tgctttgtaa
      181 tagtttttac tacgagggca gattttccca cgaggctatc tacatttgtt gcaaccttgc
      241 caacttttaa ttttcccaaa gcataggggc gagtaaaata cagcaagacg ctcgagataa
      301 tgagccatat caataattgt acggggaacg agagtccaag gagagatatg aggatcatga
      361 gaagggcacc gagggcaaac caaatcgtag ttaggctaaa ggtgaaagct tcaattgcaa
      421 caagtacaat cataataata agccatatcc acggatatga aagtgaaaga agtgttttca
      481 tacctatagt atacgtagga tacagtaatt tgtctagaaa aaaactattt ttgctaaaaa
      541 aaaatgtaaa gggctttaaa ttataaaaat atatgctata cttttatatg taaagctttt
      601 catagtaata ggaggaatta tgagtattca tattgaagca aaaaaaggcg atatagcaga
      661 tatcgtgtta ttgccagggg atccacttcg agcagaattt atagcagagc actttttaga
      721 aaacgctgtt tgctataaca gggttcgcaa tatgcttggt tttactggca cctataatgg
      781 tgttcgtgtt tctgtacagg gaacaggtat gggacagcct tccatatcga tatacgcaaa
      841 tgaactgttt gatttttatg gtgtacaaac tgcggtgcgc attgggacag ctggtgcggt
      901 gcatacttcg acaaaggtgc gagatattgt tatagcgccg agtgcttgtt cagattcatc
      961 tattttgaca caacgttttg gttccttaca ctttgcaccg acagcagatt tttctcttgt
     1021 ttataagacc tacgaaactg caaaaaaact cggcattcct gttcttacag gaccgatagc
     1081 ctcgagcgat ttattttatg atgtaaatga taactggaaa aaatgggctg agtatggagt
     1141 acttgcaatc gaaatggaag ccgctgagtt atataccctt gctgcaaaat acggcagaaa
     1201 ggccttggct attgtaacta tctctgatca tatagcaaca ggagaaacaa caacatccga
     1261 agaacgacaa acaactttta aagatatgat gaaacttgcc ttggaagcaa caacaagcct
     1321 gtagtttttc tcgaacagtt tttatgacag cgactaagac ttagcttgag gtttcgttgt
     1381 ttttatagta atatgtaacc agcatagaaa gtctcgtttt gaatgatttt gtgttgaaaa
     1441 gtaataatat ggaggagcta tgaaaccgac tttagttgtt ttagctgcag gtatgggaag
     1501 tcgctatgga ggcgttaaac aaattgatag cgtaggctta aatggagaaa ctttacttga
     1561 ctttgcctcg tatgatgctt tacaagctgg gtttggtaag attgtgtaca ttattcgaaa
     1621 ggatatagaa aaagatttta gagagcgctt tttcgatcgt gttgcaaaaa acgcaaatgt
     1681 agactatgtc tttcaagata gggatacact ttttagtgat gaagaaaaaa aacttattac
     1741 aaaccgacaa aaaccttggg ggactattca tgcgctctta tgtgcagaaa aagcggtcaa
     1801 cgaaaactat gcggttataa atgcagatga ctactatgga aaatctgcct ttgtaactct
     1861 tgggaaacat ctttcgactg tagatccttc ttcagatgaa catgcaatgg ttggttatat
     1921 tttagggaat accttgagta agagtggaac tgttagtcgt ggtgtatgcg agctaaaaga
     1981 ttcatatctc atgggtatgc gagaaaatat caaaattggc tatgaaggcg atggaataac
     2041 tggcgagctt gacggcgaaa aaaaagtata tgatggaaaa gaaattgttt ctatgaattt
     2101 ttttggcttt actcctaaat catttgcata ttttaacgaa ttttttgacg act
//
LOCUS       DAOSSW010000135         2037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_50479_length_2037_cov_3.360242, whole genome shotgun sequence.
ACCESSION   DAOSSW010000135 DAOSSW010000000
VERSION     DAOSSW010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50479_length_2037_cov_3.360242"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="PKW26_08150"
     CDS             complement(<1..147)
                     /locus_tag="PKW26_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOQ93567.1"
                     /translation="MENKIKVGVLGATGMVGQRYVYLLKDHSWFEVTFVSASPRSAGK
                     TYKEA"
     gene            complement(202..1110)
                     /gene="dapA"
                     /locus_tag="PKW26_08155"
     CDS             complement(202..1110)
                     /gene="dapA"
                     /locus_tag="PKW26_08155"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOQ93568.1"
                     /translation="MSKLQGAFTALITPFMQDSSVDYEAYKSLVRYQLEGGIDGLLPL
                     GTTGETPTLSEEEEEELIILTMKEVEAFYKKTGKKTKVIVGAGSNNTIDAVRYTERAK
                     KHAADAALVVTPYYNKPSDEGIFRHFEACAKVGIPIIVYNIAGRTGKNISTALLSRIA
                     ALPNVIGVKEASGDINQMMDVIKTIVSEKPDFSVLSGDDALTLPLMALGGDGVISVVS
                     NIAPKEVTNLVKEGLSGNFSAAKALHYRMLPFFKAAFIDGNPISIKFAMNHKGLPAGK
                     LRLPLVDIGEQYKEIVIEAMKKSQIL"
     gene            1284..>2037
                     /locus_tag="PKW26_08160"
     CDS             1284..>2037
                     /locus_tag="PKW26_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93569.1"
                     /translation="MKEHTLFGNKIPKSKARTGEFLFKHYAKKNGFDPSNPPKLSAVP
                     SEKDFDDFGILHIVEDQKASLTSTGNFADVNGPYSPIDTKNAILIGTIRMGFGHCRMA
                     MAIASAARHKGLTPYWLDMMSFPDNAASKTIRQIENWYNLGSRLSQNSQWFNRVFWER
                     ITSDAGLRLSGALRDKTISQIYAPLFANVPKGMPFVSTHPWVGHGSVAAGVEGVVTII
                     PDNYPLAFHLVEGSTHAVQSPSAYMGYRAFISM"
BASE COUNT          553 a          492 c          372 g          620 t
ORIGIN      
        1 cgcttcttta taggtttttc ctgcagatcg aggacttgca gaaacaaatg ttacttcaaa
       61 ccaggaatga tcttttaaga gatagacata tctctgcccg accataccag ttgctcctaa
      121 aacacctact tttattttat tttccataca taaccttcct tttttgtgcg ttttacgctc
      181 actgggtgaa aagccactac cctagagtat ttggcttttt ttcatggctt ctataactat
      241 ctctttatac tgctcaccga tatctaccag cggtaaacgt agttttcctg caggcaaacc
      301 cttgtgattc atagcaaact taatcgatat tggattcccg tcaataaagg ctgctttaaa
      361 aaaaggaagc atgcgataat gcagagcttt tgctgcagaa aaattgccag ataagccttc
      421 ttttacaaga tttgttactt cctttggtgc aatatttgac acaaccgaga taacgccatc
      481 accacccaag gccataagcg gtagcgttaa ggcatcatcg ccagaaagta ctgaaaaatc
      541 gggcttttcg cttacaatcg tttttataac atccatcatt tggtttatat caccactggc
      601 ttcttttacc ccgataacat ttggcaatgc ggcaatccga gaaagcaagg cggtagaaat
      661 atttttcccg gttctacctg cgatattgta gacaatgatt ggaataccaa cctttgcaca
      721 tgcttcaaaa tgacgaaata taccttcatc tgagggttta ttatagtagg gagttaccac
      781 aagcgccgca tcggcagcat gtttttttgc ccgttcagta taacgaacag catcgatagt
      841 attgttactt cctgctccaa caatgacctt tgttttttta cccgtttttt tataaaaggc
      901 ttctacctct ttcattgtta ggataattaa ttcctcttcc tcttcttcgc tgagagtcgg
      961 cgtttcgccc gttgttccga ggggaagcag tccatctata ccaccttcta attgatagcg
     1021 cactaagctt ttatacgctt cgtaatcaac gctagagtct tgcataaatg gtgtaataag
     1081 cgccgtaaag gctccctgta atttggacat agtttccatc cttatctata catttgaaaa
     1141 aagtcccgat aagacctttc taatccagtt ctaagcccgt atatattttc ttaaataatg
     1201 catagtatcg catttttata catcttattc aagactcttt ttaatttgca aaatcccact
     1261 tcatcatata tacttacttc attatgaaag aacatacatt atttggtaac aaaataccca
     1321 aaagcaaggc tcgaacgggc gagtttttat ttaagcatta tgcgaaaaaa aatggttttg
     1381 acccatcaaa tcctcccaag ctttcagcgg ttccgtcaga aaaggacttc gatgactttg
     1441 gtatcttgca tattgtagaa gaccaaaaag cctcactcac ttcgacaggc aactttgccg
     1501 atgtcaatgg tccctactcc cccatcgata caaagaatgc catccttatc ggtacaatcc
     1561 gcatgggatt tgggcattgt cgtatggcta tggcaattgc ctccgctgct cggcacaagg
     1621 gcttaacccc ctactggctc gatatgatga gctttccaga taatgccgca agtaaaacaa
     1681 tccggcaaat agaaaactgg tataaccttg gctctcgtct ttcacaaaat tcccagtggt
     1741 ttaatcgagt gttttgggag cgcataacaa gcgatgccgg cttacgactc tctggagccc
     1801 ttcgcgataa aactatttcg caaatttacg ccccgctttt tgcaaatgtc cccaagggaa
     1861 tgccctttgt ctcaactcat ccctgggtcg gacacggctc tgttgcagca ggggtagaag
     1921 gcgtcgtaac gataatcccc gacaactatc ccctcgcctt tcatctcgta gaaggctcga
     1981 ctcatgccgt gcagtcgccc tcagcctaca tgggctaccg tgcatttatc tccatgg
//
LOCUS       DAOSSW010000136         1966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_53194_length_1966_cov_7.717425, whole genome shotgun sequence.
ACCESSION   DAOSSW010000136 DAOSSW010000000
VERSION     DAOSSW010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53194_length_1966_cov_7.717425"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PKW26_08165"
     CDS             complement(<1..1171)
                     /locus_tag="PKW26_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOQ93570.1"
                     /translation="MSDAIQSPNTKDLLKIRGAREHNLKNIDLDIPRNKLVVISGLSG
                     SGKSSLAFDTIFAEGQRRYVESLSAYARQFLGRMDKPDLDLIEGLSPAISIEQKTTHR
                     NPRSTVGTVTEIYDYYRLLFARIGKAHCPNCGKEIKEQTVDQIIDTVMQWPEGERIQI
                     LSPVIRGKKGEHAKVLEDAIKDSFLRARIDGIIVELDQTIKLEKQKKHSIELVIDRIL
                     LSKEVRKRLADSIETALAISSGLLIVLRENEEVFFSQKNACPDCAISMPELQPRLFSF
                     NNPFGACPECTGLGEKMEYDFSLIVPDTSKSFNEHAFLPYNPDSAWNHCRFEAIAKSQ
                     GFSLDTPVSKLSKKQLDAIMNGTKEAIQYKYTRADGSGQSTYNQPWDGMYSDLKRR"
     gene            complement(1168..>1966)
                     /locus_tag="PKW26_08170"
     CDS             complement(1168..>1966)
                     /locus_tag="PKW26_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93571.1"
                     /translation="HQKKSKKNKARCDGDCEDPAWTEELEAELQDEDAALYYKKVLKK
                     LKKENTQWDFARVQKRARARFTRVVSGFVGHLITFIFINALLWAINFAVFEKKDDIKG
                     KIETELQIAEDSLTENEKAGIENIVNKLYEMLSEGFFPWAAIVSFAWGIGLVSNFVEI
                     FTEYKKSKELQTILPLNEEEFSLYLRIAKHRSEYIQHLCSTITIPLFLRFLHVLFSFS
                     FPWHLIVTAVLVFGLIMNSINYIPKRAKYVDRLCELKNCDNWRSLFE"
BASE COUNT          577 a          428 c          368 g          593 t
ORIGIN      
        1 accgacgttt taagtcacta tacattccat cccagggttg attgtaggtc gattgcccgg
       61 agccgtctgc ccgagtgtat ttatactgta tagcttcctt agtcccattc ataatggcat
      121 caagctgttt tttactcagc tttgatacag gcgtgtcgag gctaaaacct tgcgattttg
      181 caatcgcttc aaaacggcaa tgattccatg cagaatcggg attgtagggt aaaaaagcat
      241 gctcattgaa ggactttgaa gtatcgggga caatgagaga aaagtcgtat tccatttttt
      301 ctcccaaacc ggtacattca ggacaggcac caaagggatt gttaaaagaa aaaagccgtg
      361 gctgcaattc gggcatagaa atagcacaat cagggcaggc atttttttgt gaaaaaaaca
      421 cctcctcatt ttcccgtaag acaatcaaca aacccgaaga aatagccaag gctgtttcta
      481 tagaatctgc aagacgcttt cgcacttcct ttgaaagcaa aatacggtcg ataacaagct
      541 ctatgctgtg ttttttttgc ttttcgagtt ttattgtttg gtcgagctca acgataatgc
      601 cgtctattct cgcacgcaaa aaagaatctt ttatagcatc ttctaaaacc tttgcatgct
      661 cacccttttt accacgaatc accggagaaa ggatttggat ccgctcccct tctggccatt
      721 gcataaccgt atcgataatt tggtcaactg tttgctcttt tatttccttt ccacagtttg
      781 gacaatgcgc cttccctatg cgggcaaaaa gcaatcgata atagtcataa atttctgtaa
      841 ccgtacccac cgtagaacgc gggtttctat gcgttgtttt ttgctcgatt gagattgcag
      901 gagagagccc ctcgatgagg tccaaatcgg gcttatccat acgtccgaga aattgacgtg
      961 cataggcaga aagactttcg acatagcgac gttgcccctc tgcaaaaata gtatcaaagg
     1021 caagggagct ttttcccgaa cctgaaagcc ccgatataac gacgagcttg tttcgaggta
     1081 tatcgaggtc aatgttttta agattgtgtt cacgtgcacc gcgaattttt aaaaggtcct
     1141 ttgtgtttgg actttgtatt gcatcactca ttcaaataaa gatctccagt tgtcgcaatt
     1201 ttttagctca cataatcgat caacatattt tgcccgtttc ggaatatagt taatactgtt
     1261 cataattaag ccaaaaacca ataccgctgt tacaattaaa tgccagggga aggaaaaaga
     1321 aaaaagaaca tgcaaaaaac gaaggaaaag aggtatagta atcgttgaac aaagatgctg
     1381 aatatactct gaacgatgct ttgcaattcg tagatacaga gaaaactctt cttcattgag
     1441 cggcagaata gtttgcaatt ccttcgattt tttatactct gtaaagattt ctacaaagtt
     1501 tgaaacaaga cctatccccc aagcaaagct aacaatagcc gcccagggga aaaagccttc
     1561 agagagcatt tcataaagct tatttacaat attttctata cccgcctttt cgttttcagt
     1621 aagtgaatcc tcagcaattt gcaactcagt ttctatcttt ccttttatat catccttttt
     1681 ttcaaaaaca gcgaaattta tagcccacaa gagggcattt ataaaaataa aggtaataag
     1741 gtgtccaaca aagccagaaa caactcgcgt aaaacgcgct cttgctcttt tttgcacacg
     1801 cgcaaaatcc cattgcgtat tttctttttt aagttttttt agcacctttt tataataaag
     1861 cgcagcatcc tcatcctgca attctgcttc tagctcttct gtccatgcag gatcctcaca
     1921 gtcgccatca caacgcgcct tatttttttt agattttttt tgatgc
//
LOCUS       DAOSSW010000137         1957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_53531_length_1957_cov_6.025237, whole genome shotgun sequence.
ACCESSION   DAOSSW010000137 DAOSSW010000000
VERSION     DAOSSW010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53531_length_1957_cov_6.025237"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="PKW26_08175"
     CDS             <1..152
                     /locus_tag="PKW26_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOQ93572.1"
                     /translation="SEKYGIGFRKGDVELRDAVQDALLAMANDGTIAEISNKWFGRDI
                     SVVGK"
     gene            253..888
                     /locus_tag="PKW26_08180"
     CDS             253..888
                     /locus_tag="PKW26_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOQ93573.1"
                     /translation="MNSLLVTMLKGTVTSLEIFCLTLLFSLPLGLIIAVGRLSKKKLI
                     SSPIGVLLLIVRGTPLILQLIFVYFAPYYLFKLSYDRFTAVIVAFSLNYACYFAEIYR
                     GGIQSIPQGQYEAAKVLGLTKKQGFFRIILPQVVKRILPATGNEVITLVKDTALAQVI
                     GVAELFRVAQNAASKQFSTMPIFIAGVFYFIMNWVVSAGFARLEKKLDYYR"
     gene            898..1668
                     /locus_tag="PKW26_08185"
     CDS             898..1668
                     /locus_tag="PKW26_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOQ93574.1"
                     /translation="MCTEIIAVEHLQKSFSSLEVIKDISFNVHKGEVLGIIGPSGSGK
                     STLLRCITQLERVSSGLIRICGQTLVSNQMLKGQLKPVYASKEEMQDIALKVGLVFQN
                     FNLFPHFTVLQNVNEAQIQVLKKSPEEATENSLALLKKMSLEAKIHSYPCELSGGQMQ
                     RVSIARALALEPEVLCFDEPTSALDPELTGEILKVIRSLADEKMTMIVVTHEIAFARD
                     LCDNALFMDQGLIVEQGLAQSLITKPKNKRTQAFLSRF"
     gene            1734..1819
                     /locus_tag="PKW26_08190"
     tRNA            1734..1819
                     /locus_tag="PKW26_08190"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1768..1770,aa:Leu,seq:taa)
BASE COUNT          568 a          382 c          389 g          618 t
ORIGIN      
        1 cttcagaaaa atacggcata ggctttagaa agggagatgt agaattgcgc gatgctgtgc
       61 aagatgccct ccttgctatg gcaaatgatg gaaccattgc agaaatttct aacaaatggt
      121 ttggtagaga tatatccgtt gttggtaaat aatctcacaa aaatataaaa acaagttcat
      181 gctccgtagt ctaatttcta aaaaagttga aaaactgcgg agcttatttt ttgtaattct
      241 aggaagattt atatgaattc attattagta acaatgctca aggggacagt cacatcctta
      301 gaaatcttct gtttgacact tttattttca cttccccttg gcctcattat tgctgtcggg
      361 aggctttcaa aaaaaaagct tatcagttct cccataggtg ttttgctttt gattgtacgc
      421 ggcaccccgc ttattctaca gctcatcttt gtctactttg ccccctatta tctttttaaa
      481 ctttcatatg atcgttttac agcggtgata gttgcctttt ctctaaacta cgcgtgctac
      541 tttgcagaaa tttatagagg gggcattcaa tctattccgc aaggtcaata cgaagcggct
      601 aaggttttgg gcttaacaaa aaagcaaggt ttttttagaa taattttacc acaggtggta
      661 aagcgtattt tacctgcaac gggaaacgaa gttataacgc ttgtaaaaga caccgccctt
      721 gcacaggtga ttggcgttgc agagcttttt cgcgttgcac aaaatgccgc gagtaagcaa
      781 ttttctacca tgcctatttt tatagcaggg gtgttttatt ttattatgaa ctgggtggtt
      841 tctgcgggct ttgcgcgctt agaaaaaaaa ttagattatt atcgatagga ctttttcatg
      901 tgtacagaaa ttatagctgt agaacatcta caaaaatcat tttcaagtct cgaagttatt
      961 aaagatattt ctttcaatgt acataaggga gaagtgcttg gtattatcgg cccttctggc
     1021 tcaggcaagt cgaccttgct tcgttgtatt acgcaattgg aaagagtttc gagcggactt
     1081 attcgcattt gtgggcaaac ccttgtatcg aatcaaatgc taaagggaca gctaaaacct
     1141 gtctatgctt caaaagagga gatgcaagat attgccttaa aagtaggctt ggtttttcaa
     1201 aactttaatc tctttccgca ttttactgtt ttacaaaatg taaatgaggc acaaattcaa
     1261 gtgcttaaaa aaagccctga agaggcaaca gaaaactccc ttgcgctatt aaaaaaaatg
     1321 agcctggagg ctaaaataca ttcataccct tgtgagcttt ctgggggaca gatgcaacgt
     1381 gtttcaattg cacgagcgct tgccctcgag cccgaagtgc tttgcttcga tgagccgacg
     1441 agcgcccttg atcccgaact tacgggagaa atcctaaagg ttattcgctc cctcgccgat
     1501 gaaaaaatga caatgatagt ggtaacccac gaaatagcct ttgcacgtga tctatgcgac
     1561 aatgcacttt ttatggacca agggctcatc gtcgagcaag gtcttgcgca aagccttatt
     1621 accaagccaa agaataaaag aacacaagct tttttaagca ggttttaatt tgctttctat
     1681 tgaccaagag cctaaattac gatattatta tttttattac aaagctatta cttgccggga
     1741 tggtggaata ggtagacaca agggacttaa aatccctcgg ctgttaaagg ccgtgccagt
     1801 tcgattctgg ttcccggcat aaaaaaagct gtttccttag cgaaacagct ttttttattt
     1861 attctaccag gctatataaa tccgaaatat catttcgcca tgtgctctct ttttctttat
     1921 tttcccattc tatcaatttt cgtatttcaa attttcg
//
LOCUS       DAOSSW010000138         1801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_60368_length_1801_cov_7.423826, whole genome shotgun sequence.
ACCESSION   DAOSSW010000138 DAOSSW010000000
VERSION     DAOSSW010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60368_length_1801_cov_7.423826"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /gene="malQ"
                     /locus_tag="PKW26_08195"
     CDS             complement(<1..1523)
                     /gene="malQ"
                     /locus_tag="PKW26_08195"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOQ93575.1"
                     /translation="MKILNQNTEEKIRNSGILLHPTSLASSPGIGTLGQSAYQFIDWL
                     HEAKQSLWQVLPLGPTGYGDSPYASFSTFAGNPLLIDIDTLYKKGYLWDEDMVLPDSI
                     KNQNKAAVDFGALVQWKLPILKRAAKSYLFARVQQYKKQSDNSAKDPYAHTSYIKFVR
                     DNAYWLEDYALFMSIKDFHDKKAIEEGAENTIWFLYWPKDLAQRDEKALANWKKNNKD
                     EIEQYKIIQFFFFVQWKLLKTYANERGIKIIGDIPIFVAPDSSDVWAHKKFFQLDSNA
                     KPKAVAGVPPDYFSKTGQLWGNPLYNWKTLKKDHFRWWVQRIEAMLELVDYVRIDHFR
                     GFEAYWSVAYGEKTAVNGKWIKAPGKELFKTLQRKLGEKGKKLPIIAEDLGVITPEVE
                     SLRDSFQFPGMKVLQFAFSLDEKHKGALLNSFLPHMYSSNFVVYTGTHDNDTLCGWFF
                     SLKKEEQDLIMNYLEGVVPTNGNKDYSREQVVSLFLKEAFSSVAKSVVIPFQDLYMLG
                     "
BASE COUNT          536 a          317 c          355 g          593 t
ORIGIN      
        1 ccaagcatgt ataaatcctg gaagggaata acgacgcttt ttgcaacgga tgaaaaagct
       61 tcttttagga agagggagac aacctgttca cgagaatagt ctttgttgcc attcgtgggg
      121 acaactcctt cgagatagtt cattataagg tcttgttctt cttttttgag cgagaaaaac
      181 caaccacaaa gcgtgtcgtt gtcgtgggtt ccagtatata caacaaagtt tgaagaatac
      241 atgtggggta aaaaagaatt tagaagcgct cccttgtgct tttcatcgag gctgaaggca
      301 aattgtaaaa ccttcatgcc tggaaactga aagctatcac gcaaggattc aacttctggt
      361 gtaataacgc cgaggtcttc tgcaataata ggaagttttt ttcctttttc tcccagtttt
      421 ctctggagag ttttgaaaag ctcttttccg ggggctttta tccattttcc attgactgca
      481 gttttttcac cgtaggctac tgaccaatag gcttcgaagc ctctaaaatg atctattcgg
      541 acatagtcta cgagttcaag cattgcttct attctttgca cccaccatcg aaaatgatct
      601 ttttttaaag ttttccaatt gtaaagcggg tttccccaaa gttgccctgt tttgctaaaa
      661 taatcaggtg gcacacccgc aacagccttt ggttttgcat ttgagtcaag ttgaaagaat
      721 tttttatgag cccatacatc gctggaatcc ggtgcaacaa aaataggaat atcacctata
      781 atttttatgc ctctttcgtt tgcgtaggtt ttgagaagtt tccattgcac aaaaaagaaa
      841 aactggataa ttttatattg ttcgatttcg tccttattgt tttttttcca atttgccaag
      901 gctttttcat cgcgttgggc gaggtctttt ggccaataca aaaaccaaat agtgttttct
      961 gctccttctt cgattgcttt tttgtcatga aagtctttta tgctcataaa aagcgcataa
     1021 tcttcgagcc aataggcatt atctcttaca aacttaatat aacttgtatg tgcataggga
     1081 tcttttgcag aattatctga ttgtttttta tactgttgta ctcgggcaaa caagtaggac
     1141 tttgcagcac gttttaaaat agggagtttc cattgtacca aggcgccaaa gtcgacagct
     1201 gctttgtttt ggtttttaat agaatctggt aagaccatgt cttcgtccca aagatagcct
     1261 tttttataca atgtatcgat gtcgattaaa agagggtttc ctgcaaatgt tgaaaaagat
     1321 gcgtagggtg aatcgccata gccggtggga ccaaggggaa gcacttgcca aagactttgt
     1381 tttgcctcat gcagccaatc tataaactga taggcagatt gcccaagtgt tccaattcca
     1441 ggcgaagaag ctaacgaagt tgggtgcaag agaataccac tatttcgtat tttttcttca
     1501 gtattttgat ttaatatttt catatatata ttataaacta tctatcttta gaaatcaatt
     1561 gtgaacgttg aagaaaatta tatatgataa attgtgagtt atgaaccaca gatagacaga
     1621 gagggacaca gaaaaaatta taaaatgtaa atgatgagtt atgaatgaaa aatgattgca
     1681 aattcatgaa gattatggta ttataatatt aggaggattc aatggcaata ttacaagttc
     1741 gcgatattga tgataccttg tataattctc ttaagatatc tgcaaaaatg caaaatcgtt
     1801 c
//
LOCUS       DAOSSW010000139         1528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_77050_length_1528_cov_7.808554, whole genome shotgun sequence.
ACCESSION   DAOSSW010000139 DAOSSW010000000
VERSION     DAOSSW010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77050_length_1528_cov_7.808554"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /locus_tag="PKW26_08200"
     CDS             complement(<1..1469)
                     /locus_tag="PKW26_08200"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOQ93576.1"
                     /translation="MRYCDESYIASGDEFFIASIPLEQYVLRDYIEENRPIQDTALKY
                     FGIPYVYPWQILVVANILDAAAQNEKNSSDTGSKSTEDSEEIDDIIFDEDGVERGKQI
                     VLLPTGAGKSLCFLIPALLLKGPSLIIYPLIALMNDQERRMREGGIEPVIFRGSQSPE
                     ERERQFAALENGAKIILANPEVLQSESLIQRLAKANISHLAIDEAHCVSEWGDSFRPS
                     YLTLGAIIKKLNIKTITAFTATASQSVLSRVAEVLFEGKAHIVRSESDRKNILYYVKR
                     CVAKQKAALESAIVEQKPLIIFCSTRNRAEKTAKNLIGFFGPDKVKFYHAGLAKEEKE
                     AVEKWFFPKSDAILCATCAFGMGVDKKDIKTVIHLDPPPTVEAYIQEAGRGGRDGSNA
                     KGILLWSPKDLHEAKKHPEGSRQRALADFAESKTCRRQILLDALNAEQAACSGCDICE
                     KSHIDFAEDESRVLDFFKKHAKCYDMEEATDTIVKTCNE"
BASE COUNT          428 a          368 c          274 g          458 t
ORIGIN      
        1 ttttcgttac aggttttgac aatcgtatca gtggcctctt ccatatcata acactttgcg
       61 tgttttttga aaaaatccaa tacccgagat tcatcctctg caaaatcgat atgacttttc
      121 tcacaaatat cacaaccgct gcaagcagct tgctcagcat tgagcgcatc gagcaagatc
      181 tgtcgcctac aggtttttga ttccgcaaaa tctgccaggg ctctttgcct gctaccttca
      241 ggatgttttt ttgcctcgtg taaatccttc ggactccaga gtaaaatccc cttggcattg
      301 gagccatcac gaccgcctcg tccagcttct tgaatatagg cttcgacagt tggcggagga
      361 tcgagatgaa taactgtttt tatatctttt ttatcgaccc ccataccaaa ggcacaggtt
      421 gcacaaagaa tagcgtcact tttgggaaaa aaccattttt ccacagcttc tttttcttcc
      481 ttcgcgagcc cggcatgata aaacttcacc ttatctgggc caaaaaaacc gatgagattt
      541 ttcgctgttt tttccgccct atttcttgtc gagcaaaaaa taatgagcgg tttttgttct
      601 acgattgcag attccaaggc agctttttgc ttggcaacac aacgtttaac atagtagaga
      661 atgttttttc tatccgattc gcttcgaaca atatgcgcct ttccttcaaa taaaacttcc
      721 gcaactcgac tcagtacgct ttggctcgca gttgcggtaa aagctgtaat agtttttata
      781 ttcagttttt ttataattgc acccaatgtt aaatacgaag gacgaaaact atcgccccat
      841 tcgctgacac aatgcgcctc atcaatggca agatgcgaaa tattcgcttt tgccaacctt
      901 tgaatgagac tctcgctttg caaaacttca ggattcgcaa ggataatttt tgcaccattt
      961 tcaagagcgg caaattgcct ctcacgttct tcgggacttt gggagccacg aaaaatcaca
     1021 ggctctatgc caccttccct catgcgcctt tcttggtcat tcataagtgc aatcagcgga
     1081 taaataataa ggctcggacc cttcaaaagt agagcaggaa ttaaaaaaca aagcgacttt
     1141 cccgcacccg tgggcaaaag cacaatttgc ttccctcttt cgacaccatc ttcatcaaaa
     1201 ataatatcat cgatttcctc agaatcttcc gtagacttag aacccgtatc gctcgaattt
     1261 ttttcatttt gagctgccgc atctaaaata tttgctacaa cgaggatttg ccaaggatag
     1321 acatagggaa tcccaaaata ttttaaagca gtatcttgaa tagggcgatt ttcttcgatg
     1381 tagtcacgta aaacatactg ctcgagggga atgctagcga taaaaaactc atccccactg
     1441 gcaatatagc tttcatcgca ataacgcacc tcatcaattt ttttttcagt aaaagaatac
     1501 aagctaaaaa ctccttttag cgcgagct
//
LOCUS       DAOSSW010000140         1510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_78392_length_1510_cov_10.909966, whole genome shotgun
            sequence.
ACCESSION   DAOSSW010000140 DAOSSW010000000
VERSION     DAOSSW010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78392_length_1510_cov_10.909966"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="PKW26_08205"
     CDS             <1..1146
                     /locus_tag="PKW26_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HOQ93577.1"
                     /translation="YKSLENVFAELDIFLKAEVRLVKFVDRTFNLNEERYLAIWNYLL
                     ENHNGITMFHFEIAAEQFSDRVLDFLQKMPRGIMQFEIGVQSLNPDTLKEIHRHADIE
                     KLSKIISRIPSTIHSHLDLIAGLPHENIKSFASSFDQTIALKPDMLQLGFLKILSGTE
                     MESYAKKHVGEKGYQWMEKPPYEVLQSPDLSYAELLFLKDIEESLDIFYNSGNFKTLC
                     LYLLEKKYSLFHFFSVFVDYCQKKELFKVKHKTQGWAEIIAAFLDALEKTPETEKLYE
                     IKESLYELLRFDFMRLVKPGKIPSWYKRQYNKDAHHAALLAHTEIKSTRESYAYSDYD
                     RFAINPFSFEKISDEQEKVAILFLYAKPQERTSGQIKSETDCIVLEK"
BASE COUNT          510 a          255 c          260 g          485 t
ORIGIN      
        1 tataaaagtt tagaaaatgt ttttgctgag ctcgatattt ttttgaaagc agaagttcgc
       61 ttagtaaaat ttgtagaccg aacctttaac ctcaatgaag agcgctatct tgctatttgg
      121 aattatcttt tagaaaatca taatggcata acgatgtttc attttgaaat tgcagctgaa
      181 cagtttagcg atcgcgtttt ggattttctc caaaagatgc cacggggaat tatgcaattt
      241 gaaatagggg tgcaaagttt aaatccagat accttaaaag aaattcatcg tcatgcagat
      301 atagaaaaac tttcaaagat aatttcacgt attccgtcta cgattcattc tcacctcgat
      361 ttaattgctg gcttacccca tgaaaatata aaaagttttg catcttcctt tgaccaaact
      421 attgccttga aacccgatat gttgcaacta ggttttttaa aaatcctctc tggtactgag
      481 atggaaagct atgcaaaaaa acatgtaggc gaaaaaggct accagtggat ggaaaaaccg
      541 ccctatgaag ttttgcaaag ccccgacctt tcctatgcgg aacttttatt tttgaaggat
      601 atagaagaaa gcttagatat tttttacaat agtggaaact tcaaaacact ttgtttgtat
      661 cttttagaaa aaaaatattc actcttccac tttttttcag tctttgtaga ttattgccaa
      721 aaaaaagaac tcttcaaagt aaaacataaa acgcagggct gggcagaaat cattgcagct
      781 tttttagacg ctcttgaaaa aactcccgaa acagaaaaac tctatgaaat aaaagaaagc
      841 ctgtacgagc ttttacgctt tgattttatg cgcctcgtta aaccaggaaa aattccttca
      901 tggtataaaa gacagtacaa taaagacgca catcacgcag ccctccttgc ccataccgaa
      961 ataaaaagta cacgcgaatc ctatgcctat tctgattacg atagatttgc tatcaatcca
     1021 ttttcatttg aaaaaatctc ggacgagcaa gaaaaagtgg caattctttt tctctatgca
     1081 aaacctcaag aacgaactag cgggcaaata aagagcgaga cagattgtat tgtgttagag
     1141 aaataaatga ttctcacggt gattttcgat aatttttttt gtgaagatat tcttgtgtaa
     1201 tcaatgtttt tgtttgataa aatagatatg aaactcaaaa catttaaaac atttttttac
     1261 acgacaattg gaaaaaaaca ggcgaggact cttttagcac tatagatttt gcaacggagc
     1321 ggataaaaac tattttgacg attacaagaa aaatagaatt taaactacaa tatttatttg
     1381 acttgctttt tacttttatt ggtcgtttta ttcaacctgt aatgaatctt gtgattatcg
     1441 gtttgttggg aaaaaatgtt gtcgatggca gtatgacctt tggtgccttt gttttggtta
     1501 tcacctacta
//
LOCUS       DAOSSW010000141         1506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Treponemataceae bacterium isolate AMR_MDS_2231
            NODE_78714_length_1506_cov_4.713301, whole genome shotgun sequence.
ACCESSION   DAOSSW010000141 DAOSSW010000000
VERSION     DAOSSW010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864298
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Treponemataceae bacterium (wastewater metagenome)
  ORGANISM  Treponemataceae bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Treponemataceae.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:19:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,643
            CDSs (total)                      :: 1,606
            Genes (coding)                    :: 1,600
            CDSs (with protein)               :: 1,600
            Genes (RNA)                       :: 37
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 2 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Treponemataceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78714_length_1506_cov_4.713301"
                     /isolate="AMR_MDS_2231"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2854022"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="PKW26_08210"
     CDS             <1..1342
                     /locus_tag="PKW26_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOQ93578.1"
                     /translation="SFQWEEASTDLQVDLGLIAYTPVKKPGLRSFWKRADFPDPILDL
                     RPFSIEKNKNRIVHLTAKTSTNATPGIYNAFIYVKSNEDLLWTIPFQIKVYDLALPKT
                     ARLKTAFYYYFDEANEERYYNGAWTNEMSEAMYKKALEFRFTSPPSLSWKENAANNDW
                     TSFDQRIKYWLDAGATVFDLPVSFRRTTSIEELQTKHIPLLEKLEKHLIEKNWIDICY
                     IYYFDEPSLSSIKTIKEQLKHIKEKVAHLKIMYVYGTNRAGEKAFVDLVDIWMPNIHQ
                     YKEPFAKERQAAGDEMWIYTCIGNIHRRHPDNFRVDWYGASHRALGPWLFAHEIDGYL
                     YWRIDRWVFNPWETAETFPWANGDGMMFYPNPDKKSLPLASLRLHAMRDSFEDFDLLT
                     MLKEKGERRGYFTEEEKELLSVQELTDGKVWFEKDDNTYDTFHKRLLEALCRPD"
     gene            complement(1313..>1506)
                     /locus_tag="PKW26_08215"
     CDS             complement(1313..>1506)
                     /locus_tag="PKW26_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ93579.1"
                     /translation="TCDLFFTANCAYDVLTKLSPVDTEEVSAYELVEINSTQDIEKLP
                     LAFSSAKEALLVWAAKRFK"
BASE COUNT          498 a          306 c          306 g          396 t
ORIGIN      
        1 ttccttccag tgggaagagg caagcacaga cctccaagtt gacttaggac ttattgctta
       61 tacaccagta aaaaaacctg gtctgagaag tttttggaaa agagcagatt tccctgaccc
      121 cattttagac cttagacctt tttctatcga aaaaaataag aacagaatag tccaccttac
      181 agcaaagact tctacaaacg caacgcctgg catctataac gcttttatct atgtcaaatc
      241 gaatgaagat ttgctatgga ctattccctt tcaaataaaa gtgtatgacc ttgcacttcc
      301 aaaaacagct cgcttaaaaa ccgcgtttta ttattatttt gacgaagcca acgaggagcg
      361 atactataat ggagcttgga caaatgaaat gtcagaggct atgtataaaa aagcgcttga
      421 atttcgtttt acctcccctc cttctctttc atggaaagaa aatgcagcca acaatgattg
      481 gaccagtttt gatcaaagga taaaatattg gcttgatgca ggtgccactg tttttgacct
      541 accagtttcg tttcgtcgaa caacaagcat cgaagagctt caaacaaaac acattcctct
      601 cttagagaaa ctagaaaaac atcttataga aaaaaactgg atagatattt gctacattta
      661 ttattttgat gaaccgagtc tttctagtat aaaaacgata aaagaacagc taaaacatat
      721 aaaagaaaaa gtagcacatc taaaaataat gtatgtatat ggtacaaata gggctggtga
      781 aaaagccttt gtagaccttg tggatatttg gatgcccaat atccatcaat acaaggagcc
      841 cttcgctaaa gaacggcaag cggcaggaga tgaaatgtgg atttacacct gtataggaaa
      901 tatccaccga cgacatcccg ataactttcg agttgattgg tatggagcct cccatagggc
      961 gctcggccca tggctttttg ctcacgaaat tgacggctat ctctactgga gaatagatcg
     1021 ctgggttttt aatccttggg aaacagctga gacctttcct tgggcaaacg gagacggtat
     1081 gatgttctac cctaaccctg acaaaaaaag tctccccctc gcaagccttc gcctacatgc
     1141 gatgagagac agttttgaag attttgacct actaacaatg ctcaaagaaa aaggagagag
     1201 gcgaggttat tttaccgaag aggaaaaaga acttttgagt gtacaagaac tcaccgacgg
     1261 aaaggtttgg tttgaaaaag acgacaatac ctacgatacc ttccacaaaa ggctacttga
     1321 agcgctttgc cgcccagact aagagagcct cttttgcaga agaaaaagca agaggtagtt
     1381 tctcaatgtc ctgcgtcgaa ttgatttcaa ccaattcgta ggcactgact tcttctgtgt
     1441 caacaggaga gagttttgta aggacatcat aggcgcagtt tgcggtaaaa aaaagatcgc
     1501 aggttt
//
