LOCUS       DAOSTA010000001        70193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_413_length_70193_cov_12.471528, whole genome shotgun sequence.
ACCESSION   DAOSTA010000001 DAOSTA010000000
VERSION     DAOSTA010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 70193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70193
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_413_length_70193_cov_12.471528"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      768..845
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            895..1638
                     /locus_tag="PLU08_00005"
     CDS             895..1638
                     /locus_tag="PLU08_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70126.1"
                     /translation="MDSQNKIKKVAKLPSRKFRLFLTFLGTAVSTVALTITTLAWFMS
                     TLKGGAEIPDIGVDDEYAHSFSCALNSVPITNGSVEFTPFYPGHVHRKTLTFSLTNLS
                     SKTFKMNLFFEQPAALEEVPYIDATGKWGNANYYYYLGSQIALTSVSLAIDGASPTFT
                     SGAGNHLVETNSVGVVKGQVNGVANELTSIARFYIIQNVSVPASKTLTGTLEFTFVDN
                     GTDQSMYFVDWPTTGVSRRSLSAFFYRGI"
     gene            1640..2338
                     /locus_tag="PLU08_00010"
     CDS             1640..2338
                     /locus_tag="PLU08_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70127.1"
                     /translation="MKEKKRNIVRTIWLMVLSLVASASLTYAWHISVYNFAQTHNVPG
                     ELTPEISLAHYNYTTSSWQNSTSAEPLSIYLGKMTNIAQIPANSNTYLKIRINETSDA
                     QTKYNVIVNNIETVISNIDGVHAVTGVDYFAAENAQNVFDFYVYTSTSDNLNPLTVFA
                     NLQTMTPFKITTLPHALLSNSATLDTWIYILLKPDIAKVQNIIRLVPIQLSPYNMVFR
                     MSLNGEVKTLDENA"
     gene            2325..5552
                     /locus_tag="PLU08_00015"
     CDS             2325..5552
                     /locus_tag="PLU08_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70128.1"
                     /translation="MKTHKFKNFFSKVWISITVFFAFVLTIGYPVYAYFRANIERQVE
                     PTLEIELLFGHLREDLVTGEWGTDTNPYLIMSTDHLSNLSVLQNGAYSHYVNENSVFQ
                     VSDENGDPIYVGGATANQLFTIQSIGNEDYPFVSKIRGITTTDPNKYVTLPTGELTDT
                     SIIGNVRVTPEENQVDIGLFGCVGPDLEGEVPETDYQGEISNILLYNIQINTSTVGVM
                     PSGHYGYAKTGVTKETNHLGILAGHVQYANLDKISVYYSQTNGVAHVDAFNVSAGDTA
                     KYTTSGGIIGFYKRVIVDGEIENPVTSDGSDQGAGQAAAGLGKGIVYAEDIWKFMEEN
                     TTVGNPAPNNSYDLQDTFGDELYDPNDNSYFHVGVFTFAHSRQSRGKDRIAKIWTDAY
                     TNEWTISTNPTNPYTQTTRDVGPATKYTATRITNNEMSWNYTGWFNRQYYGTLNSPYS
                     SNADYRYMLVYEDTTVNPTKHYALVRYGSTAIGVEVDPNNLVIPDGEFKYFTYENLSQ
                     RTADITYPPYEAGFSYRFSGPITMDFRNTTSSAGTLQYAVYGDIVTGIVDGQTKILEA
                     PRPLRVNAQSNAVPTSSFMATASTSNPPYVEGYRISPTSNTGNFNKFYIQRTGASSSG
                     GSTSNWYMTYSTAEGFSAVSTSSPGTYASSFALYAVRYTNNTNNPSESPVATRNILEE
                     FIPAGTTETIDTEHNVLFYTGTYNSSVPTSRYSYEIKDISSLGWIDNRNKAITKGDTA
                     LLIGDPTSYYYIKNASGLERFFGVNTGIPSPVDGPPINVPEGSIGFTVNAKTPDSTET
                     TATINVIVATDPSQGVEQLITVSRFGSGTTTVGDRQLLSGFVLPPPPGSTITGTKPVY
                     LKTSASSPTYAVYPNFNRLLIAYSFEVPIETVPITYFLEASRGTACFVYLSSDRVAAN
                     DNNPLHENDIVIPSLPNIDYVFKNTTNMIATVGRTDYVPSMTMPYFGLTTNPNYDAET
                     NPNVDHYLVTAATGFDFVYNISRIYDPSQDEKYFLYIKVHANVNGSGYAAPVDLNSLK
                     IIQQNMNFNYSENSYLDRNTNEFIHSDVVHFTINGYFLDDWSVLE"
     gene            5653..6822
                     /locus_tag="PLU08_00020"
     CDS             5653..6822
                     /locus_tag="PLU08_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70129.1"
                     /translation="MYGGNFIKLIIPIILASLSLGGFLTPNKSLVEAKQNDATRETVV
                     YHEDFENIVGEIYQDTKFWGNGMSIVENAGNHSLLYTYDGGATQIGGINTHQTGLTGN
                     DLFENGKLYRVEFDALVTSVADNATVQFDFNIEGLWTSFKVGKTTAYKTESSNGTVRN
                     VQYVDGQVSATFTCSAGGHYFEIFVANMASGDSFKIDNFKVVCLDDVYSTDNYGTIAG
                     SSQQDHYYVADGTLEPATAASGQKYLHFYGTNNTVDTWEAMFFNHLPLTYTAGKNYRN
                     VIKFLEAPTTTEMYINMVGNWAGHTTFTINPVTGEVTHDSGLETCDITWSDNTLVIDF
                     YCSTTLPEQQIRFVSKSIAGTVSDFKIAEFTVYDLDLLTAKAFAKDLLNKTANTT"
     gene            7053..7940
                     /locus_tag="PLU08_00025"
     CDS             7053..7940
                     /locus_tag="PLU08_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70130.1"
                     /translation="MSNKGKIKLKANTAYKISFDTKGYRVGGNGAYYFLKLRSGQENI
                     MTIGEWYDRPDAPANNKIFTFTTPENSKDLTIVFGIKNEGGYYVDNISLIELPNGHIV
                     DGEDVGFDNNVRPFTPNKGLGYEEGFEDGVLADSLFSFGFNRWGHFTNKPNEVINEKL
                     SFTSELDEVTYQFQDKNNYYEFMYSNDKYLKLSGNSTYRLTLKYKLMTEIRLHSDPSL
                     KGYAYIFARSKATHVDGNPDNPLLVGTEIHFASASTLETVYTETFELMTPSCDDTMII
                     IGLYGIGTMIVDDILVEKV"
     gene            7942..8646
                     /locus_tag="PLU08_00030"
     CDS             7942..8646
                     /locus_tag="PLU08_00030"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOQ70131.1"
                     /translation="MFPNNTHKAITFSFDDGVTQDRRLVALFNKYNLKCTFNLNSGGF
                     GKDGVCHWQKWSAPHNKVNSAEVKALYSGHEVAVHTVNHPDLTTIADEKIIIDEIEQD
                     RKVLEKLTGDKIVGMAYPFGTLNDRIVNIIKRNTPIKYARSIWATYNFDLQDNLLAFR
                     PTIHFLDFKKMYELAGTFLNTEYYEDKIFYIWGHSYELDFEDAWEEFEKFLAYIANKK
                     DVFYGTNKEVLLNGQK"
     gene            8633..9562
                     /locus_tag="PLU08_00035"
     CDS             8633..9562
                     /locus_tag="PLU08_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOQ70132.1"
                     /translation="MVKNKVAVIIDTDLGADCDDVGTLVLALQLEKHGFCDIKAVGYC
                     TTNIYGVQCVDIVRSFYGGKFPIGMYYGKKVECNEDQHIFDIDLVDHYKDLIKETNYI
                     EAYKLYRKVLAEAEDDSIIFITIGELTNAANLVSSLPDEISPLSGQELFAKKVKYSCV
                     MGGLINDNHPITAFNNNEAFPEYNIVCAIEDAQKFVKISAKPIYFLDFYVGVDVFTGK
                     KVIEHDEHSPMKFAYLKYANGPSRSWDLLAVYYAITKRLYRSVGPGVMHIDDNGFTTF
                     TKDQDGHHYYLRPIKSNEEITKEIDEALVGNIL"
     gene            9688..10404
                     /locus_tag="PLU08_00040"
                     /pseudo
     CDS             9688..10404
                     /locus_tag="PLU08_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            11363..12409
                     /locus_tag="PLU08_00045"
     CDS             11363..12409
                     /locus_tag="PLU08_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOQ70133.1"
                     /translation="MRKFDYLFLKDGSVPASLIGLISSIYSLRTLDETRKHKYASIYT
                     KLIKIAKVQSVKGSNAIEGIVTSDERIKAIVNQNSAPLNHDEEEIAGYRDALNIIHNT
                     YENLQFEEKTILDLHRLLLAVSSPENAGRYKTSDNVIIEIKPDGTRNVRFRPTPTSET
                     APAIEQLILAYTIAVAESGINKLLLIPCVILDFLCIHPFSDGNGRVSRLLTLLLLYKA
                     GFDAAKYISFEEQINKRKGFYYEALRKSSVGWHENNNNYFFFIENFLVTLFYCYQELD
                     KRFLTVNSEKVNKTARIEATVLNSIVPISKREICEILPDISVTTVEYVLGKMVKSGKV
                     RKIGKSSNTKYFRM"
     gene            12440..13351
                     /locus_tag="PLU08_00050"
     CDS             12440..13351
                     /locus_tag="PLU08_00050"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOQ70134.1"
                     /translation="MKKVFVLGSINYDVVVYTDKMPILGESIIGQGMIANLGGKGANH
                     AIAAKKIGVDKVFLISAVGDDDSGHIMLKSIKEHGVDTSGIEVIKGEPSGTCFIIFNR
                     SAEDNAVIVGNGANLMNAPQKAIDLLKKYARKGDIFIAQLETNLEALYKGIDFAHELG
                     MYIIINPSPVCEFNKSILSKVDLLILNAAEAKLLTGVNYKNNNDLQEIHDKLKAKSTI
                     VTFGEDGAYLIRDNEIIYQLAIPTNVVDTTCAGDTFLGALAYRKANGYEIEDSLRFAA
                     TASSIAVSRKGATQSVPYLNEILELYK"
     gene            complement(13355..14833)
                     /locus_tag="PLU08_00055"
     CDS             complement(13355..14833)
                     /locus_tag="PLU08_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HOQ70135.1"
                     /translation="MKIISKSNYTLFKNCPKSLWLSFYKSDVAAPLTMDVLKRIEDGK
                     EVGDLAKKYFANTIDVKSLLPDGSLDISKMLELTKKYLKQKGVTIAEASFKVNNLFCS
                     IDLLRVTSEGYEIYEVKSTTEAKVEHKVDAAFQKYVLQQYGLNVINTYILHLNKDYIR
                     KGDLELDQLFTMNHLDEDTSFIEAMHNMKSDLKAIDKLLLTKAEPAETLLTRCKNCDF
                     KDYCQKDIPVPSVLDVYRLSGYKYLNKGIVTFEDLLESNVALRKRQSIQVHAYLDNKE
                     VIIDKKGLASFLKKIKYPVYHLDFESYQMPIPPCNLMKPYDQIPTQYSLHIEYEDGSL
                     EHKAFLGNSLDPRREIAESLCANIPRNACVTAYYKGFECGRLNELATLFPDLEEHLLN
                     ISKNVIDLLEPFESGYYYHKNMGKSNSIKAVLPALYPDDSELDYSALPVVHNGSEAMV
                     MYPIMLGAKKEEKERIRNGLLEYCRLDTLAMVKLLQKLRKTQ"
     gene            14918..15454
                     /locus_tag="PLU08_00060"
     CDS             14918..15454
                     /locus_tag="PLU08_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOQ70136.1"
                     /translation="MEKEVMVNGKTYTLIKLLGKGKGGYSYLATFNNKEVVVKKIHHE
                     PCEYYTFGNKIEAEYYDYQRLFKAGIRMPRLIDICKGQEIIIKEYVKGDTIFEYVKRD
                     VNIDLFIDQVREMAALAKKAGLNIDYYPTNFVVQDNLIYYVDYECNEFSEEWNFENWG
                     IKYWTRTKEFKAVLKKEQ"
     gene            15479..15955
                     /locus_tag="PLU08_00065"
     CDS             15479..15955
                     /locus_tag="PLU08_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009986389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOQ70137.1"
                     /translation="MNFNDFSHKDLRYYLAYGSNLNLAQMQERCGTSKKVGKAIIKDY
                     RLMFKGSENSNYLTIEKAKGYQVPVGVFIIQEGDEVALDRYEGFPTLYYKKDFVVELI
                     SENGKKTTINAFVYIMHEDRILGAPSEEYIRRVREGYKNFNFDEKILDEAIVFSAK"
     gene            16211..18652
                     /locus_tag="PLU08_00070"
     CDS             16211..18652
                     /locus_tag="PLU08_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOQ70138.1"
                     /translation="MEKNGQILVVSNNFNTKNAVKTALENDFILFFSTPSEMFNEVEK
                     HPELCGVIFELKDENIQSVEQFSRLYLKYPRLKLLVLASKEFYESIDEEARFGFWCME
                     TSTAADLLNKIYELKEYPLSTIEKISLVYAAFERAPFGIMIIPNDNLELALFNRKNDE
                     VLGYTGEEYKKLGLNKETHPDDLQRVAEIEKQAKLLGENSFNVTKRVIKKDGNIVWVE
                     QFISIIKSEKYEGFSRIVISWDVTKQKAVEDTLAESDRARATLLENLPGMAYRSLNDK
                     NWTMEFVSDGALALTGYRPEELIDNKTVSYYDLIVRVHRPRIRQAFDEATKSKKQFRQ
                     EYEIVTRNGTKKWVSETAKPVFDKKGNLLFYEGIITDINHMKKIEKQLKHFQQYDQKL
                     NLPNREYLIDKIQAKLDSKNYSGTIILVNLKESQRLYRSHGYQYMELLTASLVTKLKT
                     IETSRITLHYAEEDLFVFYAKYQMTREEIKKFYAELKSAIGHTISREQIRSGIGVTFL
                     HKSISIAEVYLQRARIASEFIDDENALISIHIYNDIMDQKVKREEHLRQELIAISYEP
                     GNEHKLRLMFQPIINLQTGKVRGFEALARFTSDKYGPIAPSEFIPIVENNRLIIAFGK
                     KIINLAVSFAKELLATGIHDCGIAINISVLQLLDPNFAQDLFARIESAGVPPEMIMIE
                     MTESIFSTNYEQLSEALAEIKDYGIDTAIDDFGTGFSSLARIERLNFNTVKMDRVFVT
                     PINAENAEQGIAQDIINICKKFNIQSLAEGIETKEQYDALKGLGCEFGQGYYMSRPLE
                     VDAAIEYIKNAKRKK"
     gene            18851..19159
                     /locus_tag="PLU08_00075"
     CDS             18851..19159
                     /locus_tag="PLU08_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70139.1"
                     /translation="MSVLEKIVQAELDAKELLAKTREDNTKLLSDTHDELATLRAENA
                     LAIAREINALNKKADHEIEKLATTFAAKKETITNEIEKSAKANQSKLVDKVFKDIVEK
                     "
     gene            19156..21063
                     /locus_tag="PLU08_00080"
     CDS             19156..21063
                     /locus_tag="PLU08_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOQ70140.1"
                     /translation="MIVPVKKIRLLTAISEQGKLIEALQHESKFMLFEHENDPSLPSD
                     INNELRAIERGIKALSKYKKKKFFAYHEVKQSEFNTIEAEGKALLSKINSALKTIENA
                     EKEIAELQAEIKTLTPYLNLSVATKPLSELVRTRIVIGQVPLNLFEDFAKEADEKGHI
                     FEESSRDHEFVYGAFGYEVVYESQALILLNSYRFTEVTLPLYDTKLKALLPELNDNLA
                     NKDKAILEAKDSLKALSNDEEKLRIYYDYYYNLALRKLVVTKTTERVAYVEGWVREDE
                     FETLTKNLENKVDCDIEELAVQNEEMVPTATKNNKLVKPFESITNMFSVPNPNELDPN
                     PAMSVWYWIIFGIMMGDMGYGLALLIIFGLFLKLAKPKGGLKDLATIFLYSSIPSIIF
                     GILTGSFFGVSVDLLNIVGKWFGNPNLTSVVLQPVEDPLPMLIFSLALGIAHIITGLV
                     LKIINDARKRDIKSMLADAVSWILILVGLVIAVVVKNQIVLYVAYGMIGIGAIFILLF
                     SGRDKKGIIGKALAGLGGLYNVTSYLSDVLSYSRLLALSLSTAVIAFTMNLLAGMVGG
                     IPVVGILFAAIVYLIGHVFNFAMGLLSAYVHDGRLQYLEFFGKFYEGGGYEFTPFSYK
                     LKYVNAIKENE"
     gene            21064..21537
                     /locus_tag="PLU08_00085"
     CDS             21064..21537
                     /locus_tag="PLU08_00085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOQ70141.1"
                     /translation="MTTGLVYALIGAALAAGLAGVGSAVGVSLGGKAAAGVISEKPEL
                     FGRVLILQALPATQGIYGFLVAVLIMVKIGMIGGNPIALTNEQGLALFAAALPIAIGG
                     LASGIYQGKMAAASISMTGKNPAMSARGMTMTAMVETYAILALLVSVLMYIAVPV"
     gene            21547..22158
                     /locus_tag="PLU08_00090"
     CDS             21547..22158
                     /locus_tag="PLU08_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70142.1"
                     /translation="MKKPIFERITEEAKKEAALLVKETKSEASRILKAGKDAISAQNE
                     SELTEAKAKSVERVRQYKERNEKGLDNYKEQVRQEIVVSIFDEVVNKLANLEGEELLK
                     FVTNLIAKEKVKGDEVIHVSARNFKKFVTALSSKKDATNLDLLNKKDAKYNFTLSKEP
                     THVAEGFLLSTPQYDLIFDFKEIVINYQKKHEQRIYNELFKDE"
     gene            22151..22867
                     /locus_tag="PLU08_00095"
     CDS             22151..22867
                     /locus_tag="PLU08_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOQ70143.1"
                     /translation="MNNVYLKGVLDAKISNLLTESDFATFANLSGMDRLNFFINNELV
                     STSIVTNFEALCKLALSDLKEEVSLYTKNDPLYVNYFFAHDVIKKDQGALRDFYNETY
                     EKALQRKEEALVNYLDYTHALLNILTLVRGKKRGDTTASLLANYLKQGVLSEETFSAL
                     VNGDTHTIYAYLKNNFNIDASESESTVELEEKLDEYLFNKLKILAMDSDFKPTLIYYI
                     KRKQLQIERLRNIVYTKRNG"
     gene            22871..23173
                     /locus_tag="PLU08_00100"
     CDS             22871..23173
                     /locus_tag="PLU08_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70144.1"
                     /translation="MDELVLFSNKSEAILFKAIGFLVHVVKDEEEITEILKELPKEVK
                     VIAYDTFFTSFFTEYTKNQKQLYPLYLPLPFNTEDEGKAISEMKESIRKSIGIDLL"
     gene            23186..24946
                     /locus_tag="PLU08_00105"
     CDS             23186..24946
                     /locus_tag="PLU08_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOQ70145.1"
                     /translation="MAKVTGKIVKVSGPLVVASGMKTAQVYDVVLVGDKQIIGEIIEL
                     RDDLASIQVYEETAGVGPGEPVTLTEEPLSVELGPGLMEAIYDGIQRPLTSIYELHGT
                     YIPVGVQVPKLDRKRKWNFIKTVKVGEEVEEGDELGYVNETPAVKHLILVPPGVKGKV
                     TKINEGEFTLEEVIAELETIDGKKELTMLQKWPVRKKRPYLTKKPPKEAMVTGQRVID
                     TLFPIASGGIAAIPGPFGSGKTVVQHQLAKWADADLIVYIGCGERGNEMTDVLMEFPK
                     LVDPKTGLSLMNRTVLIANTSNMPVAAREASMYTGITIAEYFRDMGYRVAIMADSTSR
                     WAEALREMSSRLEEMPGEEGYPAYLASRLSDFYERAGVVVTLGKKKRLGSITAIGAVS
                     PPGGDTSEPVSQATLRIVKVFWGLSASLAYKRHFPAIDWLRSYSLYDDDIVKHHSASF
                     PYWKDYVALLKELLQDEAALLEIVRLVGIDSLSQKDRVKLHAAQMIREDYLHQNAFHA
                     VDTYTSIEKQYGMLDLIVTWFKEANLALDEGVEYKQMENLAVNERISHIKYVEEKDFD
                     KQVKALKKDVIAAFNNLKEE"
     gene            24951..26333
                     /locus_tag="PLU08_00110"
     CDS             24951..26333
                     /locus_tag="PLU08_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOQ70146.1"
                     /translation="MAIKAFKTIKEVVGPLMVVENVSGVKFDELVRIIQSNGEERLGK
                     VLEIDNDKAVVQLFESSQGLKIDDAKAIFTGEGVTFGVSPHILGRKFDGLGRPIDGKG
                     HIIPFKRIDINGVPLNPVARDYPNEFIQTGISAIDGLNTLVRGQKLPIFSSSGLTHNR
                     LAAQIAKQATVLGRDEKFAVVFAAIGITFEEANFFSEAFEESDALDRTVMFFNLANDP
                     AIERIATPRMAITAAEYLAYELGMHVLVILTDITNYAEALREISAARKEVPGRRGYPG
                     YMYTDLASLYERAGRVKGLKGSITQIPILTMPEDDKTHPIPDLTGYITEGQIILSRPL
                     SMKGLTPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFSAYARGKDAKELSLILGD
                     SALSEIDQKYAAFADAFEREYVSQGFDTNRSIKETLDLGWKLLGMLPRQELRRVDPKL
                     VDKYYKESKE"
     gene            26337..26978
                     /locus_tag="PLU08_00115"
     CDS             26337..26978
                     /locus_tag="PLU08_00115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOQ70147.1"
                     /translation="MSTAQINPTRMELQRLRDKLATTTRGHKLLKDKQDELIHEFIPL
                     LPEYRALREKVESALSATLIRYQFACVKMSKEAINDHINDSVTKHELSAKFDRVMGVV
                     IPNLETKASHTEVNYDLLSTTSAFDDLITAIQDLFALLIELANLENTIKILVAEIEKS
                     KRRVNAIENIVIKEIEEQIRVIRMKLTDLERSNTIRMMKSKEIIMEKKFNEGK"
     gene            complement(27041..28183)
                     /locus_tag="PLU08_00120"
     CDS             complement(27041..28183)
                     /locus_tag="PLU08_00120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOQ70148.1"
                     /translation="MENFFVIVSAFFYLSIMTASFIFLNRQDDQNSRRFRVVVLLYVF
                     TILGIILDTLYRLDYGGLINLPSYAFAGVITATYVCLPVMWGLWCLYISHTSYLKEIP
                     RAYNIVVAATLLLNLALSLISLIPGTNIYFTIVDGVYARGKLFFVQTIIFLLFLALTI
                     CFYTRFFKTFGKSERFTFLLIITTLILALVGHLLNNFIPFMVMNNIFNYMILAMNVQH
                     IHASTDFLTGLANRRSLSNHLHSRMTGTGKTSSFSIIMFDLDNFKMINDTLGHDAGDD
                     ALRQFARFLRHHCDNVDFVARFGGDEFIIVLDSMSPAELKKRVAVIREQFSEYEGNKV
                     GNFKLVFSAGYFIHNKDLKISTGELIRIIDERMFRDKRKRSNGKTN"
     regulatory      28501..28578
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            28707..29546
                     /locus_tag="PLU08_00125"
     CDS             28707..29546
                     /locus_tag="PLU08_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70149.1"
                     /translation="MKNKKSIMFGALALVLLSGLSAASTTFAWFTTTRTASLTFSDAT
                     VVCSDSNLVVTYVDSLNTFGTISDEANEIKLTGANRVTDISGNGLDFYKPVWSPIEGV
                     ATNIKEVATAENYYIDFVLKIARQSDSPSATGIKVYLGENTKILPVDATKPRDVDAVS
                     AVRMAVISYADEFRASPALIELHAPVVETSPEYIIKDTASTAYSVNGYKVHDASAEVN
                     SDPFVTKLSEADGPTHQIADLSSDSEAYVGFRFWIEGTDAEATKDKALGGMFKVALDI
                     YSL"
     gene            29672..30526
                     /locus_tag="PLU08_00130"
     CDS             29672..30526
                     /locus_tag="PLU08_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70150.1"
                     /translation="MNKKNKVLLGAIGLVLLSGIAATSSTFAWFTTVRTASISYSEAT
                     VQASDGNLAIEYLSSLNNWSGGSTYDAEANKVTIAGANKVTDISGDGKTFYKPVWTTP
                     VSTASSIETVPTTGSPGVADGFYIDFTVRLSRDNDVDENGLKVYLGTGTTIAPKDPEN
                     LADTGAVKAARLAVIDHEGDVKVRWAPEQETEPKYITTGTDSLYGVSGFETTTDLNYK
                     HGEITSADSKAAADLAGFLVADLSKGVATEANVTFRIWLEGEDKDCNNDILGGVFNVN
                     IALYTFIN"
     gene            30618..31484
                     /locus_tag="PLU08_00135"
     CDS             30618..31484
                     /locus_tag="PLU08_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70151.1"
                     /translation="MNKRNKRLGTVLTLLFISAIAATSGTLAWFTTVRTAGITYGDAE
                     VYMRDSDLVVTYKSSLNSLSATSELDGITKITLTGTNKVTDISGNGINFYKPRWNSDN
                     VTAAVINTVPSTNPGDADGYFIDFTLTLSRSPGEDPLNLYVFLGQNTAILPADPANPK
                     DVGALKALRMAVISYDNNDSATGTPSVDILYAPEAEINPEYLQLGSGGAYGLTTHELV
                     PATVKSDSFETHYTIADSQAVYPEIAVLPAGTGTADVTFRVWIEGTDIHAAESIIGGK
                     FKIKLDIYSTGV"
     gene            31676..32068
                     /locus_tag="PLU08_00140"
     CDS             31676..32068
                     /locus_tag="PLU08_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70152.1"
                     /translation="MTGQEKKKKFKLPSWATYLLLGLELALMVALVTISIVALKNVQA
                     GSGGPFIKWLIGNRVWFFVLIVLPLIVLFLFNIYLLIKVMNEASSKEFKQMTKDELLE
                     EARKQALEELRKELAKQNEPAPETKPEE"
     regulatory      32167..32246
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            32434..33657
                     /locus_tag="PLU08_00145"
     CDS             32434..33657
                     /locus_tag="PLU08_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70153.1"
                     /translation="MAEEINLSEHPLLEVANDIIREEKRRKRTKKVVAVSAFIIIVFI
                     IISYLGYAIGYYIANATSLSLLSKDPEARFAQVGFRLSEPLTVTEQHKYNLQSDEGWV
                     GGEGYVYWTTQNVSPQTMSFVLEHEGYSNTNRLSPVTSGSFTKDEDPYLPLTLYEAPK
                     QNRAERHNLNLNVADPKDYFGFTVLFRLLIDEDRAGTIPLDNIAIYFNRDTKVHGQNE
                     LPKALRFGFQSGAKKDIISPGRTLLEENDYTGSIAVGGRLDLDDPRAPASLTGFYDYT
                     FGLDGAKYGESGEQIYEIAYGEFEQPLTNENWGEPLAEAEEKLEHGDVFHAVTYKGVR
                     PLINVVPQRQYYTSFQKYMLDGDKGEPLCYTNNLGVAEMNIKFWLEGWDLATTNKVVN
                     KSFGMQLHFVAKRAE"
     gene            33678..35198
                     /locus_tag="PLU08_00150"
     CDS             33678..35198
                     /locus_tag="PLU08_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70154.1"
                     /translation="MNAFFSYIRDTFESFWANFVEWLAAVFVEPWKLVSDDFGTYRTI
                     IEMYMDSFGFWGWFFFVILMILNVAVIGGLIFLLVLLIRRLVKYSSKNYDKAKLIAEV
                     QRLNKELFFAIQEKNEVLKLKAESLGLEDPSLVEGDKTSVVIDTFPKLGAVDLKYKDK
                     DTDFITPVLDREITLEQIAVRYRYFAASQLGLFYDIDLVRVLFAAMGTVKIIILEGIS
                     GTGKTSLPYSLGKFFLNDVAICSVQPSWRDRTELIGYYNEFTKKFNESEFLRAIYEAS
                     YRKDVNIVILDEMNLARIEYYFAEFLSIMEMPNPQEWYVEVTKTADEMNPVQLVNGKL
                     LIGQNIWFFGTANNDDSTFTITDKVYDRAISITLNERGQMFDAEFTEPLKIPYEHLRR
                     LFDEAQTNFPLSKANANKFSKLDEYVVNKFRIGFGNRILRQIHTFVPIYVASGGTEPE
                     ALDFMFSSKILKKFRSLNLAFMRDELSALRAFIERTFGKNTFKHSLNQIDDFMTRA"
     gene            35206..37104
                     /locus_tag="PLU08_00155"
     CDS             35206..37104
                     /locus_tag="PLU08_00155"
                     /inference="COORDINATES: protein motif:HMM:NF021352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2357 domain-containing protein"
                     /protein_id="HOQ70155.1"
                     /translation="MADENKVTIKQEQNVVAEEVAKEETAPKTKKDKPLKVAGAQEKV
                     KKAPIKDKAKKAADTKPKQTKKVASKKSDTKVEKEKVDAKPKAEKAKKAKADPKKADA
                     KPETEKVAEKALDTEAPAAKEKEPKEAPKKEAPKKAVPRRRRLSAMELRKREALKGYK
                     RFTKVRSRYYSFLNRTVKQSKPTTNLYNTIANKAPTLSGITRNEVKRFDPEFIEKIER
                     VIPSLEEIVVNPHKFINEFAEVVQVEKARKLTPRAVKYMAQNADHVSEVLDDGRVIPK
                     KILNVYVDDDLKIYENRFIMTLINRLQIFIELRHKYIREHGDTRNSDQITIKREVQVE
                     DSLFTVDATITMSVPSDDEGARESNMDLLERLENIRRRTQFLVNSRFMREMAKAVPVS
                     EPIAQTNIIRLNWAYQDAFKLWNFIHRYEELGISYTTTQAKIEFDKKYLDKINQFSLN
                     SFFTIETEHARLALSNVEQKNIRPIIEAGDLNYELSDERFAKPGLPMRVDARVETAEQ
                     EAARLKREATREKARLKREEAKRKQKEREALKKARAKERALKRKLEAAERAKRRKEEA
                     KQRQIEKNMERARLRAEKERLLAERKAYQKLMMTEAERLRRARKAVAAQATEERKKAI
                     EDAKAQAK"
     gene            37082..37891
                     /locus_tag="PLU08_00160"
     CDS             37082..37891
                     /locus_tag="PLU08_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70156.1"
                     /translation="MPKHKQNSEAAPKKKGRIGKITSVVLGAIVVLLIVLQVVGQSTA
                     KNNHGVMRYGNYQSLRVLTDSMEPTYKVDTMVFVKRVDPKTLKGPSSEGALDGDVITF
                     VRNYHGSYDLPGDKRIVTHRIHRIEVQADGRVYFRTLGDNIHAKECPESGCTLEARGD
                     WVRDTDILGKVVGQSMVLGKFSVLLANPIFMLVFAMVPLLIVFGSSLMDLLKQLKSTE
                     GSEATPSGISEQQAEFERIKEQEKLRLLIELEKEKLRKELEEGIKDETKES"
     gene            37872..39458
                     /locus_tag="PLU08_00165"
     CDS             37872..39458
                     /locus_tag="PLU08_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOQ70157.1"
                     /translation="MKQKKARRHSGKKYLWYSILCVMLLVLAFSTYVLMSFGLIPMTG
                     NKTLDIIIGILLVIISIASIVCGIIAFVYYKRNSNQIRFNYATFELVEGINSERAFLA
                     YVAKDIKRNRGKANALVAFAATRFKKIVFTRYGYEKGGEVLGLVYETFNTLKTKYPHS
                     YYGYDYNENFLIYLRDTPYEQVQEYVNDLIEEINKTLFENRNEVKFVADFGVSYCQNE
                     NDEFITAASMLQRALIAADHGRAESDLGGLTLYEPKMYERNKRNVQLGLQIERSLELE
                     QFELHYQPKYDLKLGRFAGAEALVRWRHPELGLLSPANFILFAEQSDLIIKIDHYVLE
                     KVCKQLDEWRGKGQRLLPISVNISKRTLFTGNIINHLIKVTDKYNINPMLLEIEIVES
                     PSLQDILLLLSIVKKFKNLKVKIAIDDFGTGYSSLSYIKKIPFDVIKIDKAFLDDVDI
                     DQKSRLMIKNIIDLAHILDAYVVMEGVQDERQVKMLEEMGADSIQGFYYSRPLPPEEY
                     QAFLEDNLFEANKKKKRGRK"
     gene            39458..40195
                     /locus_tag="PLU08_00170"
     CDS             39458..40195
                     /locus_tag="PLU08_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70158.1"
                     /translation="MILLLGTTPDDILYIKNRMIMKEKGAVNKDEHFYFVGEYGGKEI
                     CMTYTGHSNIMSAVLTSYMIRKFNPYLVISIGSAQSASSNLNQGDLFIAERVYIADLD
                     YNNYTNLPLRDAIHMHPYYVSEEVYIKHFELLNSATGNYKLVRGPVISVNNFVKEKAE
                     AERIIARQENYIEGPTVFDTEMGGIVACCRFYGIPWLYLRSINYEIGKEEQLISFVRK
                     GVEAQPRIGELVEQLFIFLNTSLEESI"
     gene            40213..40686
                     /locus_tag="PLU08_00175"
     CDS             40213..40686
                     /locus_tag="PLU08_00175"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HOQ70159.1"
                     /translation="MIGINHHEIENEVTATLHAISVDNDFEFQLPLDVNAKTLGVTLG
                     VPVVISLYNEAFYYQITISDYRRSNFMQVSMRLFIGEIPNPQKLYALINKLNTKLLYA
                     KVVLDEEEDNTLILEHDFTAASVEQLSESIYDFLVDVVDEDVSPLIEELLKEIRD"
     gene            complement(40773..42392)
                     /locus_tag="PLU08_00180"
     CDS             complement(40773..42392)
                     /locus_tag="PLU08_00180"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOQ70160.1"
                     /translation="MNWLKRNIRYFILSLFLLTNGLIIFEATLAGGTSGARSSLLSLI
                     LSVFVNKTVDGPETAYVPIESLTLKTKDDTTLTEGMHFYIPLGITRRITATVLPENAT
                     DKGVNWTSSDSSALEVYPGGYLEARALSENVLVTAMTNSPLHRISFYVTIHEKVAPPE
                     YEVALTKDRITIDTTTQLEVSVDESVANEYDVRKLTFFSEDESVATINKYGVIKGISV
                     GETYVGVVGDDRKFKVSVKSNDTPLISPTSITLEFDEIAYVYDKIPLNYTFDVENVTD
                     PSLTFVSSNDIVATVIKEDDNYFIKTPKIKGTATITAYLNSNFAIRATKDIIVNNVMP
                     TSVNFTNVPTELITGQRVNVGYDLNHSLAKESLSVTDKRVIFTSSDNSIARVSTGNMF
                     GTIVALKEGVVTISATSVADPTISASFSIKIIPRPFINDDNFSDFQGFVRKAVGHFFL
                     FFIDGIFGFFTFYLFLKDKKSTRLITVLSLITGVFIAALSEFIQLFVPGRAGTITDVI
                     IDSAGYLCATMLMLLIMYLINKNKSNKNVQQ"
     gene            42508..43695
                     /locus_tag="PLU08_00185"
     CDS             42508..43695
                     /locus_tag="PLU08_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70161.1"
                     /translation="MKKCQLILLPLLTMLLSGCTPLSLSEVLKPSDNSLSETDVPSTE
                     SSSEIEGAQTWSVDDDFTWQTYEYALDSYNVPLYVPTENPYIDIISDADKETFYTNNY
                     KRASSYEDAMFRTEKGLISGDIIDTPNTANYPLNHLPNRVYRTLANYRINEGVYEYTL
                     DGEYKSYTINNINGKVKKVYYGAAYVSLDDVAAYLFAFGEAPANQMMAKNSTAMNQAI
                     SLFGKYGRVNNGYYSSDVTKYKYEPSLPHTDNGGPVEADMYNYREMDFGYTYTPWGYG
                     IESDDPYNNGTKITRSTVRFVYTAYDNEGNEGAKYIPVEHRHVFLTINHYNDFFEYLN
                     YEGGWSESFGWMSAGNEYCAGMNSSKYGKGYYDFENPVPPTKYNIPQVKTLTELQSIF
                     NCL"
     gene            complement(43970..44119)
                     /locus_tag="PLU08_00190"
     CDS             complement(43970..44119)
                     /locus_tag="PLU08_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70162.1"
                     /translation="MNIKRFLMLLINAFVLFVILFMLTRTLWTLLVFVMPVVSLFLPK
                     EKSNG"
     gene            complement(44109..45095)
                     /locus_tag="PLU08_00195"
     CDS             complement(44109..45095)
                     /locus_tag="PLU08_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOQ70163.1"
                     /translation="MKLRISSVLILLIALLLPFSRQKPLVLDHNDDPFVFHLQKESLK
                     IAQFTDLHLAFGFDANDRKTLKLISNITAETKPDLIVLSGDQTLSITAPARHRQLIRH
                     MESLKTPWTFVFGNHEADYHKMERLLKVIFTMNTKYMYFKVGPLLENGGYGNFTINYL
                     YEDNPFYNLYLLDSKAELKIPGSGRLFVYDYFSEAQVDWYRNKVMIDKINNIKSTVFA
                     HIPLIQYADALLIENKDKVKGIIGERISHQEVDTGFFNAMVESTVSEAFFAGHDHLNN
                     FTFEKDGIILGYGQISGYNGYGTLRRGARIIEIDESKMFKTYVVYEDLSYEY"
     gene            complement(45092..45640)
                     /locus_tag="PLU08_00200"
     CDS             complement(45092..45640)
                     /locus_tag="PLU08_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ70164.1"
                     /translation="MKLATKRLILRPFTLNDVDAVQKYSSKESVTRYMMWGPNKYEDT
                     KRFVSSVVSRNSETPRDVFDFIVTLKDGTVIGACGLYFDEYPKPPTLGWVFDDTYWNQ
                     GYGTETGRALLAFGFNTLKVKSILATCDNENIASARIMEKIGMRYVETRPEELPKLGK
                     RFIKVYEITALEYKLNSNKVLL"
     gene            45781..46443
                     /locus_tag="PLU08_00205"
     CDS             45781..46443
                     /locus_tag="PLU08_00205"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HOQ70165.1"
                     /translation="MKQKKINNLIIDYIFNNYDKETPIFTKDIYQKYSEISRSTIRSI
                     LSRLVKENNIVRIAEGIYSLPNYDCAIGTIAISNNEIINKKYIRNDGKIFGYRTGISF
                     ANKLGLTTQTAGVDSIISNCVANKKREVKLNKTRVIVNSPRVEVTANNYKLLQILDLL
                     NDYTSFSEINLEEGASTIFKYLEGVNLERKEVEKIVNSYPREAQVNFYKIRCQDAITS
                     KY"
     gene            46421..47137
                     /locus_tag="PLU08_00210"
     CDS             46421..47137
                     /locus_tag="PLU08_00210"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOQ70166.1"
                     /translation="MLLHQNIKEFRKIVILTATHYGLQEYQIEKDYYISLFLKPLGNM
                     SKNIPIVFKGGTSLSKCYGVISRFSEDIDLSVIQNKQLTRKQKKDLKQLIIETAKEIG
                     AEVININEIESRKHFNRYILKFNSVFNSDVDTLQKLIIETMIAYNPFPAIYQKTENLI
                     LEYLKIQKKNDIIGQYQLDSFLFLTQSIERTFVDKIFALCDYHLLNKYERNSRHLYDL
                     HMIWESGLLKKKYFHHLLIM"
     gene            47399..48400
                     /locus_tag="PLU08_00215"
     CDS             47399..48400
                     /locus_tag="PLU08_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70167.1"
                     /translation="MKFKKLLLLPLAALLLNGCAIGKKVDKDVANERVSEAIQTQEEM
                     DVDSLFQEAKQDMTESVSQTNLGAETTSKVTNKGTTTLKAKDLLGENPQVVASAKATA
                     KIDKNGQEDSFVSVNENMYYVDGWFYNDYDNKERGNISLAPKSGKGKIKINVGNIDFA
                     NKNVLAVFSELSGTAVNFGPVDYSQILLLASITGNVKAREFAGVLTITYNLSKDDLVR
                     FVAAQMAGIDYGDLTSEERELVDAIISEIIAQLEKGYEVRKYVIEVKIAKEGYITKVR
                     VENDVTQTITDPQGMGEDPEYVIVVKSKGFTEINTKLNEKVAIKFPDFSDYVERSLM"
     gene            48540..49532
                     /locus_tag="PLU08_00220"
     CDS             48540..49532
                     /locus_tag="PLU08_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70168.1"
                     /translation="MKLRNLLWLPIAAMLLNSCALGKKVEPSVAQERALAATKANEEA
                     EIDTQAITSKSEISDTIKSYEGETVVTETITSKFESSFKAKGLHGDTPEFAATYKEMM
                     EEKVDGDETYYVNHEEAFYLKDSWLFGGFSTTVRERGETETEENKGKVKIVPIDFKVV
                     SVFDYLSGFSAGEYQFGAPNEEGLAILFQVASEIEAKELFGKLTITYKLDVKSLVLIM
                     LLAFGLDLDAMTEEEREAFDTELNTMVEEMSKVMSINKYQMTLGISKDGYVEKIRTDI
                     DMVTNKYNEEVEPKVLVASEELKTFTESTMEINKKLTIKYPNIDDYPEAQPIIR"
     gene            49804..50877
                     /locus_tag="PLU08_00225"
     CDS             49804..50877
                     /locus_tag="PLU08_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70169.1"
                     /translation="MKRKRMLWLPLLALLLGGCSVDFDFGPTSKDSEGGGGGTTSQTG
                     GAVSRDEANSRVTAAFTNSDIADATTYQQTTLQEVTDDTKEYVNDALKYHVVENTNSN
                     VVVKATGLQTDDPVAKVTGEPLEDMTEDGVETYKLIDNREFYYASEWIYYHNATRKRY
                     GNPEEPFIDTEVKLKQHMGPTNGQQLAEGLEGFDLGDAPVPTMFIGSELDVLQQTANI
                     TATKNGRNITVIYSINKDAFIAMMESGLRGILFSEEMTSEERAEAEEFLQWYVALLEE
                     VLDLREYKITIGADGNDHLTQVRADFELITTMVYEPSETYPKGYTQVGKLSGFLDMKP
                     KFNEEFTITLPDFSDYEEGIIFE"
     gene            51044..51544
                     /locus_tag="PLU08_00230"
     CDS             51044..51544
                     /locus_tag="PLU08_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOQ70170.1"
                     /translation="MEKWDLYTKDEKKTGKIVDKGALIPEGYYHIIVETLIKHTDGTY
                     LVMQRSYDKKEYPGKYEGSCGGSIKAGETKEEGAIREVFEESGLVINEVTPTYYFIQK
                     DVPAINQGYLAIISAKDKTIKYQKEETINHKWLTLKELKAFIKSDEYNESHASRLVTY
                     LDSLNE"
     gene            51586..53004
                     /locus_tag="PLU08_00235"
     CDS             51586..53004
                     /locus_tag="PLU08_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HOQ70171.1"
                     /translation="MKETNKKTRLKGFMKTTVLSFQHMFAMLGATVLVPMLAGLSISI
                     TLITAGIGTIIFYFVTKRKVPVFLGSSFAYLPALMSIMAESGEVGSLAWQQNMGKLVI
                     AIFFTGVMYLIFAGVIRLIGVEKVRKMFPPRVVGPVIIVLGMLLVPSIFWNNIIAPYA
                     LYGAEAWKVWTTALITLLSIIAISAFAKPKSFFKAAPILLGFLIGYVYGAIVGLVDFS
                     TLKGSDIVIFQSIGSLLGFYKYLKFDWNAILMMVPIAFVTLMEHIGDISANSTVCGKD
                     FFVDPGIDRTLAGDGFAIMAASALGGPPQTTYGENTAILVITENYEPRNVFYAAILAV
                     IFGVISIFGNVIATIPSAVIGGASIILFGMISASGLRMVIEHKADIGQTKDLIVVSVT
                     LAVGLGLGALSVASDILSAVGVTTFDPRLLKVMIGNVELSPLAIATLVAVLLNLVIPN
                     PKVVKSEEKKASTEDTLTNEIN"
     gene            complement(53006..54013)
                     /locus_tag="PLU08_00240"
     CDS             complement(53006..54013)
                     /locus_tag="PLU08_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ70172.1"
                     /translation="MPTIRDIARKANVSPATVSRVLNGDPTINVQAKTKRRIFEVAED
                     LAYTKHLQPKKNQQKNKFRIAIYHWYTIEQEINDPYFISIRIGVQRACRELGIAFDVI
                     YREDRITDPFEDQKYDGVIMIGSFTRETQTFISDRFSAVVYANSEDEDGKCDCVRVDF
                     RALTHDVLHLLINKGHKKIAYIGGRERVSSEPNKTPDPRELEYIEVMHQNKLYYKPYV
                     KVGEYSAHSGYELANELIDNNEGNTPTAIFCGNDSIALGVSKAIQERGFKIPDDIAIF
                     GVNDIPTLQFTSPSLSSVKIHTEFMGELALKLLNERMKKERSLKLSVNVPYELILRES
                     A"
     gene            54298..55755
                     /locus_tag="PLU08_00245"
     CDS             54298..55755
                     /locus_tag="PLU08_00245"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOQ70173.1"
                     /translation="MFCYHKTSASNNEKFFRKGIFLSITLLIATTVLTSCKGGTSVNS
                     EIRSSEFNNNEVVYPSNPAFGNNVTAHDPSIFKDDDGTYYTFGSHFAVTKSINLMQWT
                     QVANDGGANVLYARNWRTVLADAFAHVGERPGSTWAPCVHKFGEKYYMYYSLSTFGSS
                     VSYIGRVEGDSVLGPYDNSVEIVKSDGRGGPNAIDPELFFSKDGRLWLVYGSFFAGIY
                     IKELYASGENIGLPKEDGYGKLLWQGGDQGPEGPFIFYNPNTDYFYLMASHGSLSTNY
                     NMRVARSKNPDGPYTDPRGMSAENYRNSGLKLAGNYQFQGYPIGYAALGHNSILREDG
                     KYYVVYHSRYRSGASGVTGYHNQFVNQMFFNKNGWPVLAPNRYAGEELQVITMAQASK
                     DYDVLIHTGGDSEGFATSVTYKFSQNGDLLDAEEDAIGTWSINEDYYVTIKIGLNTYE
                     GVIIPQWNNDLQRAGLSITAYSRNGLSLWANEHFR"
     gene            55795..56961
                     /locus_tag="PLU08_00250"
     CDS             55795..56961
                     /locus_tag="PLU08_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70174.1"
                     /translation="MKKRRLLIPLIGLALLLTGCKTTSSEEKPVSSEQPPVTSVTTEQ
                     PSVSSEEPPASSEETPVANIKFGADPFNGTSATGVAGVGVALYYDATEDDVAVAFDKV
                     AFTVNKPSGVLFDPVIGLVEKLSVYYLRPMEPGTYTVTLTVEDSAGLKATATKEIVVS
                     TGDLSGDVDAIKAAIAAIYPDNIYTNLDYRTSEGVFNEWVVVGKNFTVFDREDYTGNY
                     ATAFIPFARFEGGEALQDFTISFKYTTLNEVWKLLFSFWTGEVGADNFAGDYLRLLTN
                     RNAIGIQGDAQQASEFQDEGEATGIPLKEGPVWIKFTRQVTKVGEGDYTVVFKLFTST
                     DGETFVNNTTATLVNQVNTAGGTAGLLTGFLPFSIDNDFIVEDLAVSGTVFTLE"
     gene            57030..58343
                     /locus_tag="PLU08_00255"
     CDS             57030..58343
                     /locus_tag="PLU08_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70175.1"
                     /translation="MKKLRYLLILLSASLVLSGCKGGKVSEEETLPELTNMTVDGVIN
                     EEAYNVIPGRTAGVKAGVLVQYAVGREGVYFGLTVTDTKHQVSAPSGIVASDYVGLAV
                     DALAVPKSDDGVSESTKLFRVDTMGRYTYTTGNEYGAWVDVDSGVYEGVISGEEIPTI
                     AVNIVKDQYYVVEIFFTWEHLGTTEEAAKDANCLMYYIEHRDMGVDIHADANILAPAM
                     YNRLVYLGNRKGSNLPADPPEITIDGVLDETLWDTAFVTNSGNFKERVPDETAGDYRT
                     LAIWGEKGIYLGVEVEDPHIEAPNGTGAAYKNAGMEMRIHVYNKDGLPLNSLKWLFDV
                     WGPQWHETGAGGLASSFAPYAEYKFVISGTIDNNEDTDEGWSFEIYIPFSELGVTNVD
                     SDYIKILHAVGSHEQNNMLPQEYLDLHPDVNWDYPEDYPRISKPV"
     gene            58357..59802
                     /locus_tag="PLU08_00260"
     CDS             58357..59802
                     /locus_tag="PLU08_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ70176.1"
                     /translation="MFKKKWMPLLLSIPLLLTGCKGKTSDSVTIFIYGQAHERSIYSK
                     IIEKFTEETGIKVNAELVNTDGYAETLTASLGTKNAPDVFYSNPASIGRYAMNGVILD
                     LKPYLEIEAEVEDGLDYKNFLDGVVDYYRYDVTTKKRGEGEAVWAIPKDVSSYPLGYN
                     RNIINLSVELGHWATHTKKGAWASREGGMPLPWENDPRTDKQIVYTFEEFTNACRATK
                     FIHNEIEYFGTALIDTWAMHSWIWAAGGNWLSDDGKTVTVNTPEFIAGFTEFIDLMDN
                     RGISQTREQMGVKPHYDRWMAGEVAFFNVGVWDVAAFESVPKELLDYGLMPTPRKDNA
                     SPEWYTYIGTLGYAVSSRTRVAQEAVKLALYMSMSTESYNRMTKYQTIQLPNNKLMVD
                     EYLSDETIKPENKGIFLDVIQKGHGKLHPTAMAYNSLWYDAFIAELSRVWKVTDDDPS
                     GEMSPAQYAAYVQPIMQAQLNIALADEAAGV"
     gene            59872..60780
                     /locus_tag="PLU08_00265"
     CDS             59872..60780
                     /locus_tag="PLU08_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ70177.1"
                     /translation="MNTNIVEKPKKRKVKHVQENIIGYLFILPVFIGIAFFIVYPFFY
                     SLVSTFKNWNGLVPLSVAPTVGFEMYRRVLSDSLFWQAFGNTLITLIGIPIGMILSIA
                     IAMLLNRGLKFTGIFRVIFYIPVVSSITAIIILWRAMMNTQGPINDFLALFGVKAINF
                     LGEPGWARFSLIMMLVWKGLGTSILLYLAGLQGISDTYKEAAKIDGANDWQIFWHVTF
                     PLLGPTHFFIVVTGIIGGMQLYVEPELLLGGGPSRATTTLLIYLFERFGAHRISEASV
                     VAWVLGVFVFIITAIQFYTNYRREKA"
     gene            60777..61631
                     /locus_tag="PLU08_00270"
     CDS             60777..61631
                     /locus_tag="PLU08_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013523906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ70178.1"
                     /translation="MKKSNIKANRSIKRAKLFSDISTYVILVLGSVLMLAPLIWMLSA
                     SLKVNQFSVYGPLFHEWNFSNYIEVFKTIPVWKGLLNSVIVAVPSIIVGTFVSGLAAY
                     AFAKIKFKFCNIIFILMLAVMMIPFPVIMIPQYYIFSDLDWIGTLLPLIIPKLFGNIM
                     MIFFLRQFLTHIPDALVESAKIDGANHFQIFWIIILPLLKPALAAQTVLWFIGIWNDY
                     LAPNFFSAASPTLPVVIGGLVSDFPIQEPVHLNMAASIISMIPVLAIFAIFQKQIINS
                     FVLSGMKE"
     gene            61646..63124
                     /locus_tag="PLU08_00275"
     CDS             61646..63124
                     /locus_tag="PLU08_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HOQ70179.1"
                     /translation="MKTKLFIDKNLTIGKVSNLLFGSFIEHLGRAVYTGIYEPGHPKA
                     DKDGFRQDVIDLIKELKVPMVRYPGGNFLSGYNWEDGIGPKENRPVRLDYAWQTIEDN
                     TFGTDEFMRWCKKAEIEPMMAVNLGTGTPKDAGNIVEYCNFPKGTYYSDLRIKNGHKE
                     PYNIKYWCLGNEMDGPWQICHLNAEDYAKKALETAKIMRWVDPSIKLIACGSSNAEIE
                     TYPDWDRIVMETLYDHVDYISMHRYYWYEDSLEDFFASYHDMDNFIHTIKSTADFVKA
                     KKRSDKVMKISFDEWNVWYLNKWKPGGWKKAPHILEDHYSLKDALVFSGLLNTLVNNV
                     DRVEIAALAQLVNVIAPIFTKEGGGVFKQTIFYPFYAVSNYGRGVALKAIKTGPKFNS
                     KFGEVNYVSEAVTFNEEKREISVFLVNYAQEKMEVELELRSFGRVEALTHKVMTHDDL
                     EIKNTFDEENVKFVEGELPTVKNDVATLELGPLSYHVYRFKY"
     gene            63137..64765
                     /locus_tag="PLU08_00280"
     CDS             63137..64765
                     /locus_tag="PLU08_00280"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HOQ70180.1"
                     /translation="MKRYLFLISVAILALTACSGGKTITSISNSDNIEEAIIYPTKPY
                     SDSDHIMQKDPYAFYNIAGNDTRYWGAANTHDPAIIKEGDTYYAFSTDANFGVTSQKG
                     IHIRKSNDLIKWSFVGTALDVTTLEDQIEYIGYNRDGEKVDFFWAPDIIKRGNEFWLF
                     YSISGFGQRNSMMGLAKSNNIEGPYTFDKEILRTSQSVGGTPNAIDSAIFVEEVDGEE
                     KMYMSYGSWSAGIYIIELDPVTGYPLIAQTLVEREVTVNTAEKGVTATATKLVPSSSD
                     DPAFGTKILSITSSEAPYIIKENGYYYLFITNGVDLTYDYDVRVFRSRNILGPYVDGK
                     GVSALSATNYNSFRPYGNKVTIAHQFTYLTDDISYKRGWAGIGHSTAFKDEEQWFFAS
                     HYRGTYMDRHRFFLGIWKMHFVNDWPVIEPNRYTGEGSINLEQADISGNYRIQILHQS
                     AVNSMLTDDIISIVNRAEEITLTKQKAGEWYVVNGEYNGRYRLNNNQLELELNGVLYK
                     GVITPQFNYEFEKGVLGLSLINSDGTALWGNRII"
     gene            64778..66082
                     /locus_tag="PLU08_00285"
     CDS             64778..66082
                     /locus_tag="PLU08_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOQ70181.1"
                     /translation="MYLGIEFGSTRIKGIVLNDNFEVIASGSYIWQSTLKDGYWTYDL
                     NEVWVGMNAVIDEIAKTINVKEIKSLGVSAMMHGFLPFDKNGKLLTPFRTWKNTNTTV
                     AATELSKQFAFNIPLRWSIAHLYQAYLDKESYVKDIDTFTTLAGYVHLTLTGKNVLGI
                     GDASGVFPIDVKTKHYNQKMIQTFNDLTDMKLEKLLPGIVEVGEDAGFLTAKGAELLG
                     NKLTVGIPLAPPEGDAETGMVSTNALKPKTGNVSAGTSVFSMIVLEKALNKWYPEIDI
                     VQTPTGDAVAMVHANECTSRIDPWIELFGEAIMRVNGEVDRNHLFETLYEVSLEDTPL
                     GEFMKQKLVEAIIDLKPGMDILLNKEHVKVDYLLGHGGYFKSKSAGRKVMSETLGIPI
                     RLLETAGEGGPWGMAVLAAYRYYNKEQKIKFDKFIEKVFKGA"
     gene            66086..67522
                     /gene="araA"
                     /locus_tag="PLU08_00290"
     CDS             66086..67522
                     /gene="araA"
                     /locus_tag="PLU08_00290"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOQ70182.1"
                     /translation="MKTFWFIVGSQSLYGEETLKQVEKNAKKIAKGLKTKYPIVYKET
                     VKTHAEAMRLIKDANYDENCLGIITFCHTFSPSKMWVNALQNLQKPWCHFHTQFYDEI
                     PNDAIDMDYMNLHQSAHGDREHGFIGTRLQKNRKIIVGYWQDPKTSEAISAWQDVVDA
                     VNESKTLSVVRFGDNMREVAVTEGDKVEAQMKFGWQVNTIGVGTLVDTIKAVTNEELT
                     AKLAEYKAKYALNTDNFASIEYQAKAEVAIKKILDANNAKAFTNTFEDLYNMEQLPGL
                     ATQNLMLEGYGYGAEGDWKTAAMTRIFKAIGKVTTFMEDYTYDWKEGLILGAHMLEVC
                     PSIARERPKIEVHHLGIGGKKAPARLVFEGKPGKAKAISLIDLGHRFRLIALDVECVA
                     PTKSMPNLPVARLMWRPEPNLEKSAACWLVAGGAHHTTLVYDLSLEFLRDFANLLDIE
                     FVHIGKDADPYILEEMMKNNDIRYRLRG"
     gene            67526..68215
                     /gene="araD"
                     /locus_tag="PLU08_00295"
     CDS             67526..68215
                     /gene="araD"
                     /locus_tag="PLU08_00295"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HOQ70183.1"
                     /translation="MYKTLKETTYNANIAIKKSGLVLLTWGNVSVKDDVLKVIAIKPS
                     GVAYEKMKADDIVVVDFDGNIIEGTLRPSVDLPSHLALYKAHQEIGAIVHTHSTFATA
                     WAQKGRAIPMYGTTHADYFSDDIPCAPYLTPEEMKDYEKNTGEVIAALIKKGRANETP
                     AAIIKGHGAFAWGKNAKDAVLHAIVLEEVAKIAYLTEAIPGQNNPLPEHIKKTHYERK
                     HGPNARYGQKK"
     gene            68308..68769
                     /locus_tag="PLU08_00300"
     CDS             68308..68769
                     /locus_tag="PLU08_00300"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOQ70184.1"
                     /translation="MAIYLYSKGDKLPPGHFHIIVETLVKHSDGTYLVMQRDYSKKEY
                     PGLYEGSAGGSLISGETKKEGAIREVREETGLIINDVSPTYYFMSEKDGVISQGYIAI
                     ISGTKDITYLKGETINHKWLTLKELKAFILTAEYNKAHASRLKTYLDTLED"
     gene            68809..69261
                     /locus_tag="PLU08_00305"
     CDS             68809..69261
                     /locus_tag="PLU08_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOQ70185.1"
                     /translation="MTNRKERTKGFFKTTILSFQHMFAMLGATVLVPMLVGLDVSITL
                     ITAGIGTIIFYFMTKKKVPVFLGSSFAYLPAMMAIMSGSGPIGSTLWQQKMGALVVAI
                     FFTRLVYLVFAGVITLIGVERVRKIFPPRVVGPIIIVLGMLLVPNFME"
     gene            69347..>70193
                     /locus_tag="PLU08_00310"
     CDS             69347..>70193
                     /locus_tag="PLU08_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOQ70186.1"
                     /translation="MSAFAKPKSFFKAAPILLGFFVGYIYAAIIGLVDFSSLQNADII
                     VFQSIGDKLGFYRYLRFDWSAIIMMVPLAFVTLMEHIGDISANSTVCGKDFFVDPGIN
                     RTLAGDGVAIMAASLLGGPPQTTYGENTAILVITENYEPKNIFYAAVLAIFFGVFSIF
                     GSVISTIPSEVIGGASIILFGMISASGLRMVIEHKADIGQTKDLLVVSITLAVGLGLG
                     ALSVASDILAQVGTDFDATLLKIMIGNIELSPLAVATFVAVFLNLVIPEPKVKAETPI
                     LENEVK"
BASE COUNT        23805 a        11382 c        13900 g        21106 t
ORIGIN      
        1 agaaagaaaa aacttcatga agcaggagtt gatgtgtctt taaaagaatt atttgaaatg
       61 tatcataaaa taagtggaca aaaataatat cgcttatgtg ctgataccct aaatgtagac
      121 acacatacaa caaaagaact atatattgga tcttagcaaa tgtaaaaact atttttggaa
      181 atatcgtgaa gcccttactt tagaaattat aaattgtgat tttgaaataa gtaagtaatg
      241 attgtcgaat aatcatttta taaaagaatg cgcgactatt tatttaaatg cgtaaaaatt
      301 taagtgcaac tgaaaaaaat acgaaaaata ttagttgtag taacacttaa aaaaaaataa
      361 ttgggatagt tgaaatcaat taaatgtgaa atataaacga aaagaaactc aaaatatgac
      421 ctaacagaag cccaaaatgt gagcgaatgg acccttaaaa taaaaccggc aatgttgatg
      481 aagccgctaa taagttagtg aaaataaaaa agcgttagaa taagaagggg tcaaaggacc
      541 agacatttta gtcgttattt gcggattaag tcgttatgct tataaacgtg aagatggtgt
      601 ttatgttgtg ccaattacta ctttaaagta ttaaaaaaca tatcatatta acgatatttt
      661 ttaatgtttt gaataatgaa cgaagttgaa taagttttat ttcttagaaa aataccgata
      721 aatctacaaa aattattgac attattcata tttatgaata taataatgct agaaaaaggc
      781 aaaactagag caatctagcg acgcaaagct ataggatctt ggaaacgaga tagccagcta
      841 cctaacagag aggtagcttt ttttacagga aaaggctatt aagagaaagt gaacatggat
      901 agtcaaaaca agatcaagaa agtagcgaaa ttaccaagtc gaaaatttag actatttttg
      961 acatttttag gtaccgctgt ttcaacggtc gcgcttacta ttacaacgct agcatggttt
     1021 atgtcgacgc ttaaaggggg cgctgaaatt cctgatatcg gtgttgatga tgagtacgct
     1081 cattcgtttt cctgtgctct taatagcgtt cctattacga acggaagtgt tgaatttaca
     1141 ccattttatc cgggtcatgt tcatcgtaaa acattaacat tctcgctcac taatttaagt
     1201 agcaaaactt ttaaaatgaa tttatttttt gaacaaccag cagcgcttga agaagtgcct
     1261 tatattgatg caacgggtaa atggggtaat gcaaattatt actactattt aggtagtcaa
     1321 attgctttaa cttctgtttc acttgcaatt gacggtgctt caccaacttt cacaagtggt
     1381 gcaggcaatc acttagtgga aacaaatagt gttggtgttg ttaaaggaca agttaatggt
     1441 gtcgcaaatg aattaacatc aatagcacgc ttttatataa ttcaaaatgt ttctgtgcct
     1501 gcgagtaaaa ccttaacagg tactttagaa ttcacttttg tggataatgg cactgaccaa
     1561 agtatgtatt ttgttgattg gccgacaaca ggtgtatcaa gaagatcgct aagtgctttc
     1621 ttctatcgag gaatataaaa tgaaagaaaa gaaacgcaat attgttagaa cgatttggtt
     1681 aatggtgctg tcacttgtgg caagcgcttc tttaacatat gcgtggcata tttccgttta
     1741 taattttgca caaacacata atgtacctgg tgaacttacg cccgaaatct cactagcgca
     1801 ctacaattac acaacgagta gttggcaaaa tagtacgagt gcagaaccgc ttagtattta
     1861 tttagggaag atgacgaata tcgcacagat tcctgctaat agcaatactt atttaaaaat
     1921 aagaatcaat gaaactagtg atgcacaaac taaatacaat gtgatagtaa acaatattga
     1981 aacagttatt tccaacattg atggtgttca tgcagttact ggtgttgatt atttcgcagc
     2041 agaaaatgca caaaatgtct ttgactttta tgtctatact tcaacaagtg ataatcttaa
     2101 tcccttgact gtatttgcga acttacaaac aatgacccca tttaaaatca caactttgcc
     2161 acacgcatta cttagcaata gtgcgacttt agatacatgg atttacattt tgttaaaacc
     2221 agatattgct aaggttcaaa atattattcg gcttgttcca atccaattat caccatataa
     2281 tatggtgttt agaatgtcat taaacgggga ggttaaaaca ttagatgaaa acgcataaat
     2341 ttaaaaactt cttcagtaaa gtatggattt caattaccgt tttctttgct tttgttttaa
     2401 caattggcta tccagtatat gcttatttcc gcgctaatat tgaaagacaa gtagaaccaa
     2461 cgctagaaat tgaactactt tttggtcatt tacgtgaaga tctcgtaact ggcgaatggg
     2521 gtacagacac taacccatat ttaattatga gcaccgatca cttatcaaac ctttcagttt
     2581 tacaaaatgg tgcttatagt cattatgtta atgaaaacag cgtgttccaa gtttctgatg
     2641 aaaacgggga cccaatttat gttgggggag caaccgcgaa ccaattattt acgattcaat
     2701 caattggcaa cgaagactat cctttcgtat ccaaaattag agggattacc acaaccgatc
     2761 caaataaata tgtgacatta ccgactgggg aattaaccga cactagtatt attggtaatg
     2821 ttcgtgttac accagaagaa aatcaagttg atattgggtt atttggttgt gttggtccag
     2881 atcttgaagg cgaagttcct gaaaccgact atcaagggga aatttctaat atccttttat
     2941 ataacataca aattaacact agcaccgttg gtgtcatgcc aagcggccac tacggttacg
     3001 ctaagacggg ggttactaaa gaaacaaacc acttaggaat ccttgcgggt cacgttcaat
     3061 acgcaaattt agacaaaatc agtgtttatt attcacaaac aaatggcgtt gcccatgttg
     3121 acgcctttaa tgtcagcgct ggtgacaccg ctaaatatac aacttccggc ggaattattg
     3181 gtttttataa acgcgttatt gttgacggtg aaattgaaaa cccagtgacg agtgacggta
     3241 gtgatcaagg tgctggtcaa gctgcagcgg gtttaggtaa ggggattgtt tacgctgaag
     3301 atatttggaa attcatggaa gaaaatacga ctgttggcaa tcctgctccg aataattctt
     3361 atgatttaca agacactttt ggtgacgaac tttatgatcc aaatgataat tcatatttcc
     3421 acgttggtgt atttactttt gcccacagtc gtcagtcacg cgggaaagac cgtatcgcta
     3481 agatttggac agatgcttac actaatgagt ggacaatttc cactaatcca acaaatccgt
     3541 atacacaaac aacgcgcgac gtcggtcctg cgacaaaata taccgcaaca cgcattacta
     3601 ataacgaaat gagttggaat tatacgggat ggttcaatcg gcagtattac ggaacactaa
     3661 actcaccata tagtagtaat gctgattacc gttatatgct tgtgtatgaa gacacgactg
     3721 ttaatccgac aaagcattat gcacttgtgc gttatggttc tactgcgatt ggtgtggaag
     3781 tagatcctaa taacctagtt ataccggacg gtgaatttaa atatttcact tatgaaaact
     3841 taagtcaaag aactgctgat attacttatc cgccttatga agctggattc tcttatcgtt
     3901 ttagtggtcc gatcacaatg gactttagaa acactacttc aagtgcaggg actttacaat
     3961 acgccgttta tggcgacatt gtaaccggaa tagtggacgg acaaactaaa attttagaag
     4021 caccgcgccc attacgggtt aatgcgcaaa gtaatgcagt gccaacatca agcttcatgg
     4081 caacagcgtc tacgagcaac ccgccttatg ttgaagggta tcggattagt cctacttcaa
     4141 atacgggtaa ttttaataag ttttatattc aacggactgg tgctagtagt agtggtggaa
     4201 gtacttcaaa ctggtatatg acttattcaa ctgccgaagg ctttagcgct gttagtactt
     4261 caagcccagg aacttacgcg tcttcctttg cactttacgc tgtgcgttac acgaataaca
     4321 cgaataatcc aagtgaaagc ccagtggcaa cccgtaatat tttagaagaa tttattccag
     4381 caggaacaac agagacgata gatactgaac ataatgtgct cttttacacc ggtacttata
     4441 attcaagtgt accgacctca cgctatagtt atgaaattaa agatattagt tctctcgggt
     4501 ggattgataa ccggaataaa gcaattacaa aaggggacac tgcactatta atcggtgatc
     4561 ctacttctta ttactatatt aaaaacgctt ctggccttga gcgtttcttc ggtgttaata
     4621 ctgggattcc ttcgccggtc gacgggccgc ctattaatgt gccagaaggt agtattgggt
     4681 ttacagttaa cgctaaaacg ccagattcaa cagaaacaac agcaacaatt aatgttattg
     4741 tcgctactga cccaagtcaa ggcgttgaac aacttattac tgtaagccgt tttggtagtg
     4801 gaaccacaac tgttggtgac cggcaattat taagcggctt tgtattaccg ccaccgccag
     4861 gttcaacaat tactggcact aaaccagtgt atttaaaaac gagtgcatca agcccgacgt
     4921 atgctgttta tccaaacttt aacaggctct taattgccta ctctttcgaa gtaccaattg
     4981 aaacagtacc aattacatat ttccttgaag catcgcgcgg aacagcgtgt ttcgtttact
     5041 tatcaagtga ccgcgtcgca gccaatgata ataacccgtt acacgaaaac gatattgtca
     5101 tcccgtcttt accaaatatt gactatgtat ttaaaaatac gacaaacatg attgcgacag
     5161 tcggtagaac agattacgta ccatcaatga caatgccgta ttttggatta acaaccaatc
     5221 ctaattacga cgctgaaact aatccaaatg tcgatcatta tcttgtcaca gcggcaacag
     5281 ggtttgattt tgtctataac atttcacgaa tttatgatcc aagccaagat gaaaagtatt
     5341 tcctatatat taaagtacat gcgaatgtta acggtagcgg gtatgctgca ccagttgatt
     5401 taaatagttt aaagattatt caacaaaaca tgaactttaa ctatagtgaa aactcttacc
     5461 tagatcgtaa cacgaatgaa tttattcata gtgatgttgt gcatttcacc atcaacgggt
     5521 atttccttga tgattggtcg gttttagaat aaataaaaat aagaagtaat aacttattaa
     5581 caaaacaaca aaactacatc aatacgcaac aacaatgttt ttattacgct tttcatgtat
     5641 gttagaatgg agatgtatgg aggtaatttt ataaaactta taattccaat aattttagcg
     5701 tccctttcgc ttggcggatt tttaacgccc aacaaaagcc tagtggaagc taaacaaaat
     5761 gatgcgacac gcgaaacagt cgtttatcat gaagactttg aaaatattgt tggtgaaatt
     5821 tatcaagaca ccaaattttg gggcaacggg atgagcattg tcgaaaatgc tggcaatcat
     5881 tcacttttat acacttatga tggtggagct acacaaatag gtggtattaa cacacatcaa
     5941 acaggtctta ctggtaatga tttatttgaa aacggtaaat tatatcgtgt cgaatttgat
     6001 gcattagtaa caagcgttgc ggataatgcg acagtgcagt ttgactttaa tattgaagga
     6061 ttatggacaa gttttaaagt cggcaaaact actgcataca agaccgagag ttcaaatggt
     6121 acagtccgaa atgttcaata tgttgatgga caagtttcag caacctttac atgttcggca
     6181 ggtggacact attttgagat atttgttgct aatatggcaa gcggcgattc tttcaaaatt
     6241 gataatttca aagtagtgtg ccttgatgat gtctattcaa cagataacta tgggacaatt
     6301 gcaggttcat cacaacaaga tcattactat gttgcggatg gcactttaga acctgctact
     6361 gcagcaagtg gacaaaaata tttacatttc tatggtacaa acaacactgt tgatacatgg
     6421 gaagcaatgt tctttaatca cttaccacta acatatacgg caggtaaaaa ctatcggaat
     6481 gttattaaat ttttagaagc tccaaccaca accgaaatgt atattaatat ggttgggaac
     6541 tgggccggac atacaacatt tacgattaat ccagtaacgg gtgaagttac ccatgatagc
     6601 ggactagaaa cttgtgatat tacgtggagt gataacacat tagtgattga tttctactgt
     6661 tctacgacat tacctgaaca acaaattcga tttgtttcaa aaagcatcgc gggtacagta
     6721 tctgatttca agattgctga atttacggtt tacgatttag atttattaac cgccaaggcg
     6781 tttgcgaaag atttattaaa taagacggca aataccactt agaaatagcg ggatcaggta
     6841 tttatcaaaa taaaataagt tataaaaata ttaagataaa caaggtcagc agcagtctca
     6901 ttaattttga agggaaatat aatattgaaa ctgactcgtg gtatacagac agtatttata
     6961 ctgatattta tcgcgatgaa gaaaaagaca ttaatggttc aaatgttgtc ttaatcgaaa
     7021 gtaatgtttt gcatggtgag caacaagggc ttatgagtaa taaaggaaaa atcaaattaa
     7081 aagcgaacac cgcttataaa ataagctttg acactaaagg atatcgcgtt ggtggcaacg
     7141 gtgcttatta tttccttaaa ttacgctctg gacaagaaaa tatcatgaca attggggaat
     7201 ggtatgatag gcccgatgcg cccgcgaata acaaaatatt tacatttacc acgccagaaa
     7261 acagtaagga tttaacaatt gtctttggta ttaaaaatga gggcggatat tacgttgata
     7321 acattagcct tatcgaatta ccaaatggtc atattgttga tggcgaagat gtgggctttg
     7381 ataataatgt tcgaccattt acaccaaata aaggtcttgg ctatgaagaa gggtttgaag
     7441 atggtgtttt agcagatagc ttattctcat ttggttttaa tagatggggt cactttacaa
     7501 ataagcctaa tgaggtaatt aatgaaaaat tgtcatttac aagtgaatta gacgaggtta
     7561 catatcaatt ccaagataag aataattact atgaattcat gtactcaaat gacaagtatt
     7621 taaaacttag cggaaattca acttatcgtt taactttaaa atataaattg atgacggaga
     7681 ttaggctaca tagtgatcct tcactaaagg gctatgccta tatctttgca cgaagcaagg
     7741 ctactcatgt cgacggtaat cctgataatc cgttacttgt gggaacagaa attcattttg
     7801 ccagcgccag tactttagaa acagtttata ccgagacctt tgagctaatg acgccaagtt
     7861 gcgatgacac aatgattatt attggcttat acggcattgg gacaatgatt gttgatgata
     7921 ttttagtgga gaaggtttga gatgtttcca aataatacac ataaagcgat aactttttcc
     7981 tttgatgatg gggtaacaca agatcgccgc ctcgttgcat tatttaataa gtataattta
     8041 aaatgcactt tcaatctaaa tagtggtggt tttgggaaag atggtgtctg tcactggcaa
     8101 aaatggagtg caccacataa caaggtaaat agcgcagagg ttaaggcttt atatagcggc
     8161 cacgaagtag cggttcacac tgttaaccat cctgatttaa caacaatcgc tgacgaaaaa
     8221 atcatcattg atgagattga acaagaccga aaggtgttgg aaaaattaac aggcgacaaa
     8281 atagttggga tggcgtatcc ttttggcaca ttaaatgata ggatagttaa tattattaaa
     8341 agaaatacgc cgattaaata cgcaagaagt atttgggcga catacaattt tgatttacaa
     8401 gataacttat tagcgtttag gccaacaatt cattttttag attttaaaaa gatgtatgaa
     8461 ctagcaggca cttttcttaa cactgaatat tatgaggata agatatttta tatttggggg
     8521 cattcttacg aattggattt tgaagatgcg tgggaagaat ttgaaaaatt cctagcgtat
     8581 attgctaata aaaaagatgt tttttacggg acaaacaaag aggtgttact aaatggtcaa
     8641 aaataaagtt gctgtcatta ttgatactga tttaggcgct gattgtgatg atgttgggac
     8701 ccttgtgtta gcgttgcaat tagaaaagca tgggttttgc gatattaagg cagtcggtta
     8761 ttgtacgacg aatatttatg gggtgcaatg tgttgatatt gtgcgttctt tctatggcgg
     8821 aaaatttcca attggaatgt attatggcaa aaaagttgaa tgcaacgaag accaacatat
     8881 ttttgatatt gatttagtcg atcattataa agatttaata aaagaaacta attatattga
     8941 agcatataaa ctttatcgta aagttcttgc tgaagcagaa gatgattcaa ttatttttat
     9001 cacgattggt gaactcacta atgcagcgaa tttggtaagt tctttacctg atgaaatatc
     9061 accattatct ggccaagaat tatttgctaa aaaagtaaag tatagttgtg taatgggtgg
     9121 attaataaat gataatcatc ctattactgc ttttaataat aatgaagcgt tccctgaata
     9181 taatattgtt tgtgcgattg aagatgcaca aaagtttgtt aaaatctcgg caaaacccat
     9241 ctatttttta gatttttatg ttggcgtcga tgttttcaca ggaaagaaag taattgaaca
     9301 tgatgaacat tcaccaatga aatttgcata tttaaaatac gctaacgggc catcgcgaag
     9361 ttgggattta ttagcggttt attatgcaat tactaaacgc ttatatagat cggttgggcc
     9421 tggggtaatg catattgatg ataacggatt cacgacattt actaaagatc aagatggcca
     9481 ccattattat ttaagaccaa ttaaaagtaa tgaagaaatt actaaagaaa tagatgaagc
     9541 actagtgggg aacatattat gaaaaagtca aaagatgaaa aaagaaacaa aataattgaa
     9601 cataaaaaac gagagctaaa ttattataac gttgcggcgc gcggttttga agaatcaaag
     9661 aaactaaata ttgcaccgaa agatgccatc atttccttac aaaatgtaaa taaaatatat
     9721 cctaattttg ttcaagctgt ttttgatttt tcgttagata taaagccgca agaatttatt
     9781 gtctttgtcg gtccttctgg ctgtggtaag tccacaacac taagaatgat cgcgggctta
     9841 gaggaaataa ctgcgggtga tttatatatt gatggcaaat acgctaatga cattgaacct
     9901 aaaaatagaa acatatcaat ggtttttcaa agttatgcat tgtacccaaa tatgactgtc
     9961 cgccaaaacc tatcatttgg cttagaaatt caaaatctaa aaaaagatgt gattgctgaa
    10021 agagtggaaa aagctgcaag aatattagaa attgacgaat atctagatcg aaagccgtca
    10081 gcactaagtg gtggacaacg acaaagagta gcgcttggaa gaccgattgt taaagcttcc
    10141 cctgtatttt taatggatga accactatca aacttagacg ctaaattacg gattcaaatg
    10201 cgcagtgaga ttgtttcgat tcataatcgg attggagcaa cgacagttta tgtaactcat
    10261 gaccaaactg aagcaatgac aatggcaacg cgtattgtcg taatggataa agggcgtgtt
    10321 caacaaattg gcacaccaga agaagtttat tcacatccga acaacttatt tgtagcaaca
    10381 tttattggtt cgcctgcgat gaatgctgtt aacgctattt atgataatgg ggttattaaa
    10441 tttgaagatg gttacaaatt ctcattagat aaagataaaa ataatttagt catgtcattt
    10501 atcaataaac agataaaaga atatgaagag ttgttgctag aaacatccaa aaaattagac
    10561 aagcgaaaac tttatctgtc ttcaaaagcc gaacgctata aagttaactc cttgtttaaa
    10621 cttgaaagaa agtatgcacg aaaagaaggt ttgcaccgca caaacatttc taacctagaa
    10681 aaagctttag cggatggtga caccgatggt attcaagaag gaattgatta cttgaaaaga
    10741 aaattagatg tgcttcttac ccgcaaagac actgaattaa aaacaataaa tagtaattat
    10801 caaactttgc ttgcaaaaga agcaggaaca gaagataaac atgcaaggca aagaatagaa
    10861 aaagacgatg aaattgaagt ttataatcaa gaaataactg attatatgaa aaatatcgat
    10921 tattataagg aatgtaaaac gaaaaaaaga ctaccgataa aattcggtat ccgtccagaa
    10981 gatattaaac caaagattgc tggtgaaacc ttcgaaaatg aagcaccaga aataaaagca
    11041 acaatcgatt ttgttgaatt gttgggttat gaatattatt tatactttaa atttaatggt
    11101 atgaaattta aatcaaaaga gaatgttggc aagcataaat atgtaatggg agaagaacat
    11161 acactaacat ttgatgaaaa taagatgagc ctctttgatt ttaaatctgg tctttcatta
    11221 tttaaaatta atacttaaat tatgtaaaag aaaatttctg cataaaaatt tatcaaaatt
    11281 atgcagacga ccacataaaa taattgcatc tctgcataat aattagtaaa atatatgcag
    11341 agaataaagg ttgttatgca gtatgagaaa atttgattat ttgtttttaa aagacgggtc
    11401 cgttccagcg agcttaatcg gattaatttc gagtatttat tctttacgaa cacttgatga
    11461 aacaagaaag cataaatatg catcaattta tacaaagctt ataaaaattg ctaaagttca
    11521 gtcggttaaa ggtagtaatg cgattgaagg gattgttact tctgacgaaa gaattaaagc
    11581 aattgttaat caaaatagcg cgcccttaaa tcatgacgaa gaagaaattg ctggatatcg
    11641 cgatgcatta aatattattc ataatacata tgaaaattta caatttgaag aaaagacaat
    11701 attagattta catcgattgc ttttagcggt ttcaagtcct gaaaatgcag gtcgttataa
    11761 aactagtgat aatgtaataa tagaaattaa acctgatgga acaagaaatg ttaggtttag
    11821 accgactccg acaagtgaaa ccgcacccgc aattgaacaa ctaatacttg cttacactat
    11881 tgccgttgct gaaagtggga ttaataaatt attgttgatc ccgtgcgtga tattagattt
    11941 cttgtgtatt catccattta gtgatggtaa tggacgggtt tctagactct taacattatt
    12001 attgctttat aaagctggtt ttgacgctgc caagtatatt tcttttgaag agcaaattaa
    12061 taagagaaaa ggattttatt atgaagcact taggaaatcc tcggttggct ggcatgaaaa
    12121 taataataac tatttctttt ttattgagaa ttttttagta acgctctttt attgttatca
    12181 agaattagat aaaaggtttc taaccgtaaa cagtgaaaaa gtcaataaga ccgcgcggat
    12241 tgaagcaacg gttttaaatt caattgtacc gatttcaaaa cgtgagatat gtgaaatatt
    12301 accagacata agtgtgacca ccgtcgaata tgttttagga aaaatggtta agagtggtaa
    12361 agtcaggaaa attggcaaat catctaacac taaatatttc cgaatgtagt gtgtaaaaaa
    12421 taacggaggc aattaagtaa tgaagaaagt attcgtgtta ggatcaatta actatgatgt
    12481 agtagtgtat accgataaaa tgccgattct tggtgaatca ataattggtc aaggaatgat
    12541 tgctaattta gggggcaagg gcgctaatca cgcgattgcg gcgaaaaaga ttggtgttga
    12601 taaagtattt ttaattagtg cggttggtga tgacgatagt ggtcatatca tgcttaaaag
    12661 cattaaagaa catggtgtag acactagcgg gattgaagta attaaaggcg agcctagcgg
    12721 gacatgtttt attattttta atcgtagcgc ggaagataat gctgtcatcg tcgggaatgg
    12781 cgctaatttg atgaatgccc cccaaaaagc aattgactta ttaaagaaat atgcgcgtaa
    12841 aggcgatata ttcatcgcac aattagaaac aaatttagaa gcgctatata aaggaataga
    12901 ttttgcacat gaattaggaa tgtatattat tattaatcct tcaccagtct gtgaatttaa
    12961 taaaagcata ttgtcgaaag tagatttatt aattttaaat gcggcagaag ccaagttatt
    13021 aacaggggtt aattataaaa ataacaacga tttgcaggaa atacacgata aattaaaagc
    13081 aaagtcaacg attgttacat ttggcgaaga tggtgcgtat ttaattagag ataacgaaat
    13141 aatttatcag ctggcaatac ccacaaatgt tgtggacacg acatgtgcgg gtgatacttt
    13201 tttaggtgca ttagcgtatc ggaaagcaaa tggttacgaa atagaagatt cattacgctt
    13261 tgccgcgacc gcttcaagta ttgcagtatc aagaaaaggg gcaacccaat cagtgcccta
    13321 ccttaatgaa atacttgaac tatataaata acacttattg tgtttttcgt aatttttgca
    13381 agagtttaac cattgctaaa gtatctaagc gacaatattc taagagtccg ttacggattc
    13441 tttctttttc ttccttcttt gctcctagca ttattggata cataaccata gcctcgctcc
    13501 cgttatggac aacgggcagc gcactataat caagttcaga atcatcagga tatagcgctg
    13561 gtaaaaccgc cttgatggag ttacttttac ccatgttttt atggtaataa tatccgcttt
    13621 cgaatggttc taatagatct atgacattct tacttatgtt aagtaaatgc tcttctaaat
    13681 caggaaataa tgtggcgagt tcatttaagc gaccacactc aaagccttta taataggcag
    13741 taacgcatgc attacgcgga atattagcgc ataatgactc agcaatttcg cgacgcggat
    13801 caagtgaatt accaagaaag gctttatgtt ctaaacttcc gtcctcatat tcaatatgta
    13861 atgaatattg tgtgggaatt tggtcgtatg gcttcataag gttacatggt gggattggca
    13921 tttggtagga ttcaaaatct aaatgataga caggatactt aattttcttt agaaaagagg
    13981 caagcccttt tttatcgata atgacttctt tattatctaa ataggcatga acttgaatac
    14041 tttgtcgttt tcgcaaagca acattgcttt ctaataaatc ttcaaaagtg acgatgcctt
    14101 tatttaaata tttgtaacct gataggcggt aaacatctag tacgcttgga acaggaatat
    14161 ccttttgaca ataatcttta aagtcacaat ttttacatct tgttaataat gtttcagcgg
    14221 gttcagcttt tgttaacagt aatttatcaa tagcttttaa atcacttttc atattgtgca
    14281 ttgcttctat gaaagatgtg tcttcatcta gatgattcat ggtaaatagt tgatctaatt
    14341 ctaaatcacc tttacgaata tagtctttat ttaaatgcag aatatatgtg ttaataacat
    14401 ttagtccata ttgttgtaat acatactttt gaaatgcagc gtcaacctta tgttctactt
    14461 ttgcttcggt cgttgattta acttcataaa tttcatagcc ttcactagtg acgcgtaata
    14521 agtctataga acagaagagg ttatttactt taaaagaagc ttcagcgatt gtgacacctt
    14581 tttgttttaa atatttttta gttagttcaa gcattttaga aatgtctagc gaaccatctg
    14641 gcaataaaga cttaacatca attgtgtttg caaaatactt tttagcaagg tctccgacct
    14701 cttttccgtc ttcaattcgt tttaaaacgt ccatggttaa aggcgctgcg acatcacttt
    14761 tataaaaaga taaccacagt gacttcggac aatttttaaa tagtgtgtaa ttacttttcg
    14821 atataatctt catattattt attttaccag ctttcacctt caaaagcgat agtggtcttt
    14881 agtttaaggg ttaaaaacgc tatactgatg gtgaactatg gaaaaagaag taatggtaaa
    14941 cggtaaaact tacacactaa taaaattgct cggtaaaggg aaaggtggtt attcctattt
    15001 agcgactttt aataataaag aagtcgtggt taaaaagatt catcatgaac catgtgaata
    15061 ttatactttt ggcaacaaga ttgaggcaga atattacgat tatcaacgat tattcaaggc
    15121 gggcattaga atgccgcgct taattgatat ttgtaaaggc caagagatta tcattaaaga
    15181 atatgttaaa ggcgacacaa tttttgaata tgtaaaaaga gatgtaaata tcgatttatt
    15241 tattgatcaa gttcgagaaa tggcagcttt agccaaaaaa gcgggcctaa atattgatta
    15301 ctaccccacg aattttgttg tgcaagacaa cttaatatat tacgttgatt acgaatgtaa
    15361 tgagttcagc gaagaatgga attttgaaaa ttggggcatt aaatattgga cacgcacgaa
    15421 ggaatttaaa gcagtgctaa aaaaagaaca ataaatacaa ggtaatgggg aaaatattat
    15481 gaattttaat gattttagtc acaaagattt gcggtactat ttagcctacg gcagcaatct
    15541 taatcttgca caaatgcagg agcggtgtgg aacatcgaaa aaagttggta aagcaataat
    15601 taaagactat cgcttgatgt ttaagggaag tgaaaatagc aattacctaa cgattgaaaa
    15661 agcaaagggt tatcaagtgc cagttggtgt ttttattatt caagaaggtg acgaagtagc
    15721 gcttgaccgt tatgaaggat ttccgacact ttactataaa aaagattttg ttgttgaatt
    15781 aataagtgaa aacggaaaga aaacaacaat taatgccttt gtctatatca tgcatgaaga
    15841 caggattctt ggcgctccta gtgaggaata tataagaaga gtacgcgaag gatataaaaa
    15901 ctttaacttt gacgaaaaaa tattagatga agcaattgta tttagtgcaa aataaccacg
    15961 aaatgcaagg gaaataacat tgttttgcaa gataaaactt ttaatattat tatgattttt
    16021 gagtatatgt tgctaaagaa aatgcaaaac atttatatat tttattaatt tttatattgc
    16081 aattttttta aatttttaaa aattttaagg agcgaaaaat caccgaaaaa actcaataat
    16141 atttattttt ttcaaaaaac aaaaaaatat gtataacaga tgttataatg tataatagaa
    16201 acgggatcaa atggaaaaaa acggacaaat tttagttgtc agtaataatt ttaataccaa
    16261 gaacgcagtc aaaactgcgc ttgagaatga tttcatatta ttttttagta cgccatcaga
    16321 gatgtttaat gaggttgaaa aacaccctga actctgtggc gttatttttg aattgaaaga
    16381 cgaaaacatt caaagcgttg agcagtttag tcgtctatat ttaaagtatc ctcgccttaa
    16441 actattagtg ttagcgagta aagaatttta tgagtcaatt gatgaagagg cacgttttgg
    16501 cttttggtgt atggaaacaa gcaccgctgc ggacttatta aataagatat atgaattaaa
    16561 agaatatccg ctttccacaa ttgaaaaaat atcactggtg tacgctgctt ttgaacgtgc
    16621 accgtttggg atcatgatta tccccaacga taatttagaa ctagcgcttt tcaatcgaaa
    16681 aaatgatgag gttcttggtt atacaggcga agaatataaa aagttgggat taaataagga
    16741 aactcatcct gatgatttgc aaagagtagc ggaaattgaa aaacaagcta aactacttgg
    16801 agaaaatagt ttcaatgtca cgaaacgcgt tataaaaaaa gacggtaaca tcgtatgggt
    16861 tgaacaattt atttcgataa taaaatcaga aaaatatgaa ggattttcac ggatagtaat
    16921 atcttgggat gtcacgaaac aaaaagcggt tgaagacaca ctagcggaaa gtgatcgcgc
    16981 tcgcgctact ttattagaaa atttaccagg tatggcgtat cgctcactta atgataaaaa
    17041 ttggacaatg gaatttgtgt ctgatggagc gcttgcctta acggggtatc gacctgaaga
    17101 attaatcgat aataagactg tttcctatta cgatttaatt gtgcgtgttc atagaccaag
    17161 aattcgtcaa gcatttgatg aagcaacaaa gagtaaaaag caattccgcc aagaatatga
    17221 aatagtgacg aggaatggca ctaaaaaatg ggtaagtgaa actgcaaaac cagtatttga
    17281 taaaaaaggt aatttattat tctatgaagg gataattacc gacattaatc atatgaagaa
    17341 aattgaaaaa caactcaaac attttcaaca gtatgatcaa aaacttaatt taccaaaccg
    17401 cgaatattta attgataaaa tacaagcaaa attagatagt aaaaattata gcggaacgat
    17461 tattttagtg aacctaaaag aaagtcaaag attatatcgt tcacatggct atcaatatat
    17521 ggaattatta acagcttcgc ttgttactaa attaaaaaca attgaaacaa gcagaataac
    17581 actgcattat gctgaagaag atttgtttgt attttacgct aaatatcaga tgacacgcga
    17641 agaaattaaa aagttttacg ctgaacttaa aagtgcgatt ggtcacacta tttcgcgcga
    17701 acaaattcgc tctggtatcg gtgtaacatt cttacataag agcattagca ttgcggaagt
    17761 gtacttacaa agagcacgga ttgcaagtga atttattgat gatgaaaatg cgcttatttc
    17821 tatccatatc tacaacgata taatggatca aaaagtgaag cgagaagaac acctccgtca
    17881 agagttaatt gctatatctt acgagccagg caatgaacat aaattacgac tcatgttcca
    17941 accgattatt aatttgcaaa ctggtaaagt acgtggcttt gaagcacttg cgcgattcac
    18001 tagtgataaa tatgggccta ttgccccgtc agaatttatt ccaattgtcg aaaataatcg
    18061 tcttattatt gcttttggta aaaaaattat taacttagcg gtttcctttg caaaagaatt
    18121 gctcgcaaca gggattcatg attgtggtat cgcaattaat atttccgtat tacaactctt
    18181 agatccaaat tttgcccaag acctttttgc acgcattgaa tcagcgggtg tcccgcctga
    18241 aatgattatg attgaaatga ctgaatcaat tttctctaca aactatgaac aattaagtga
    18301 agcacttgct gaaattaaag attacgggat tgatacggca attgatgact ttggtactgg
    18361 cttttcttca ttagcgagaa tcgaaaggtt gaatttcaac acggttaaaa tggaccgcgt
    18421 ttttgtgaca ccaataaatg ccgaaaacgc tgagcaaggt attgcccaag atattattaa
    18481 catttgtaaa aagtttaata ttcagtcact cgcagaaggc attgaaacta aagaacaata
    18541 tgatgcccta aaaggcttgg gctgtgaatt cgggcaaggg tattatatga gccgcccact
    18601 tgaagttgac gcggcaattg aatatataaa aaacgctaaa cgcaaaaaat agttttaact
    18661 attatgttct tatctaaaat aaccgcatag tgcggttttt tagtatacat tcaacatttt
    18721 agcggaagca catagtcgta tcacattaca ttagcaatgt aaacatgtta aaatactaca
    18781 cgtggataca aaactctacc tatttatata aataaattgg tagcggacaa aatgcctaga
    18841 aaggagtcct atgagtgtat tagagaaaat tgtacaagct gaacttgacg cgaaagaatt
    18901 actcgctaag acaagagaag ataacacgaa attattaagt gatactcacg atgaattagc
    18961 aacacttcgc gctgaaaatg ctttagcaat tgcgcgtgaa attaacgcgc ttaataaaaa
    19021 agctgatcac gagattgaaa aactagcgac aacttttgct gcgaaaaaag aaacaatcac
    19081 taatgagatt gaaaaaagtg caaaagcaaa ccaaagtaaa ctagtggata aagtctttaa
    19141 ggacattgta gaaaaatgat tgttccagta aagaaaatta gactattaac cgcaatcagt
    19201 gaacaaggta agcttattga agcattacaa cacgaaagca agtttatgct ttttgaacat
    19261 gaaaacgatc caagccttcc tagtgatatt aataatgaac ttcgagcgat tgagcgcggg
    19321 attaaagcgc ttagcaaata caaaaagaaa aagttttttg cctatcatga agttaaacaa
    19381 agtgaattta acacgattga agcggaaggt aaagctttgc tttctaaaat taatagcgct
    19441 ttaaaaacaa ttgaaaacgc tgaaaaagaa attgcggaat tacaagcaga aattaaaaca
    19501 ttaacgccgt acttaaattt aagtgttgcc actaaaccac ttagtgaact agtgcgaaca
    19561 cggattgtta ttggacaagt cccgcttaat ttatttgaag attttgctaa agaagcggac
    19621 gaaaaaggtc atatttttga agagtcatca cgcgaccatg aatttgtcta tggcgctttt
    19681 ggttatgaag tagtgtatga aagccaagcg ctcattttac ttaattcata tcgttttact
    19741 gaagtaacac taccactata tgatacaaaa cttaaagcac ttttgccgga attaaatgac
    19801 aatttagcga ataaggacaa agctatttta gaagcaaaag atagtttaaa agcgttaagt
    19861 aacgatgaag aaaagttacg gatttattac gactattatt acaatttagc actgcgtaaa
    19921 ctcgtggtaa ctaaaacgac tgaacgcgtc gcttatgttg aaggttgggt gcgtgaagat
    19981 gagtttgaaa ccttaacaaa aaacttagaa aataaagttg attgtgatat tgaagagtta
    20041 gcagtgcaaa acgaagaaat ggtgccaact gcgacaaaaa ataataagct agtgaaacca
    20101 ttcgaatcga tcacaaacat gtttagtgtt cctaacccta atgaacttga tcctaacccc
    20161 gcaatgtcag tgtggtattg gattattttc gggattatga tgggtgatat gggttatggt
    20221 ctagcgctgc taattatttt tgggttattt ttaaaactag caaaacctaa aggcggactt
    20281 aaagacttag caactatctt tctttattca agtattccct cgattatttt cgggatactt
    20341 actggcagtt tctttggtgt ttctgtcgat ttattaaata ttgttggtaa atggtttgga
    20401 aatccaaact taacttcagt agtactgcaa cccgttgaag atccgctgcc gatgttaatc
    20461 ttttcactag cgttaggaat tgctcatatt attacgggtt tagtactaaa gattattaat
    20521 gatgcgagaa agcgtgatat taaatcaatg cttgctgacg ctgttagttg gattttaatc
    20581 ttagtgggat tagtgatagc ggttgttgta aaaaatcaaa ttgtgctcta tgtagcttac
    20641 ggcatgattg ggattggcgc tatttttatc ttattattta gcggacgcga taagaaaggg
    20701 atcattggta aagcgcttgc tggtctaggc ggattataca atgttacaag ttacttaagt
    20761 gatgttttaa gttattcacg gttattagcg cttagtttaa gtacagcagt tattgctttt
    20821 acgatgaatc ttttagcggg catggttggc ggtattccag tcgtcggcat tttatttgcg
    20881 gcgattgtgt atttaattgg ccatgtcttt aactttgcaa tgggccttct aagtgcctat
    20941 gtccatgatg gacggttaca atacttagaa ttttttggga agttctacga aggtggcggt
    21001 tatgaattta cgccgttttc gtacaaatta aaatatgtta atgcaataaa ggagaatgaa
    21061 taaatgacaa caggattagt gtatgcgctc attggagcag ctcttgccgc aggtcttgcg
    21121 ggcgttggtt cagcagtagg tgtttcttta ggtggtaaag ccgccgctgg tgtaatctca
    21181 gaaaaaccag aattatttgg tcgggtgtta attttacaag ccttaccagc tacacaaggg
    21241 atttacgggt tcctcgtcgc cgtcttaatt atggttaaaa ttgggatgat tggtggaaac
    21301 ccgattgctc ttactaatga acaaggttta gcattatttg ccgctgcttt accgattgcg
    21361 attggtgggc tagcttcagg catttatcaa ggtaagatgg ctgctgcttc aattagcatg
    21421 actggtaaaa acccagcaat gtctgcgcgt ggtatgacaa tgacagcgat ggttgaaact
    21481 tacgcaattt tagcgttact tgttagtgtc ttaatgtaca ttgcggtacc ggtttaagga
    21541 gcgcttatga aaaagccaat ctttgaacga attactgaag aagctaaaaa ggaggctgca
    21601 cttttagtta aggaaactaa aagcgaggcc agccgtattt taaaagcggg aaaggacgct
    21661 atttccgcgc aaaatgagtc cgaattaacg gaagctaaag caaaaagtgt cgaacgcgtt
    21721 cgccaatata aagagcgtaa cgaaaaagga ctggacaact ataaagaaca agttcgtcaa
    21781 gaaattgtcg taagcatctt tgacgaggtt gttaataaat tagcgaattt agaaggtgaa
    21841 gaattattaa aatttgtcac taatttaatt gctaaagaaa aagttaaagg tgatgaagta
    21901 atacatgttt ctgcgcgcaa ctttaagaaa tttgtgaccg cgctttcaag taagaaagat
    21961 gcgacaaacc ttgacttatt aaataagaaa gatgccaaat ataactttac attaagtaaa
    22021 gaaccgacac atgttgcgga aggctttttg ctaagtacac cgcagtatga cttaatcttt
    22081 gacttcaaag aaatcgtcat taactatcaa aagaaacacg agcaaaggat ttataacgaa
    22141 ttatttaaag atgaataacg tttatttaaa gggagtactt gacgctaaaa tatcaaatct
    22201 cttaacagag agtgattttg caacttttgc aaaccttagc ggtatggata gacttaactt
    22261 ctttattaat aatgaacttg tttcgacaag tatcgttacg aactttgaag cgctatgtaa
    22321 gctcgcgctt agtgatttaa aagaagaagt tagtctttat acgaaaaacg accccttata
    22381 cgttaattat ttctttgccc atgatgttat taaaaaagac caaggtgcac ttcgtgattt
    22441 ctataatgaa acatatgaaa aagcgctaca aagaaaagaa gaagccttag taaattattt
    22501 agattatacg cacgcgctct taaacatatt aacactagtg cgtggtaaaa aaagaggcga
    22561 caccaccgcg agtttattag cgaattactt aaaacagggt gtgttaagtg aagaaacttt
    22621 tagcgcttta gtaaatggtg atactcacac tatctatgcg tatttaaaaa acaacttcaa
    22681 tattgacgct agtgaaagcg aatcaacagt ggagttagaa gaaaagttag atgaatactt
    22741 atttaataag ttaaaaatac tagcgatgga tagtgacttt aaaccaacat taatttatta
    22801 cattaaacgt aagcaattac aaattgaaag gttacgcaat attgtgtaca cgaaaaggaa
    22861 cggataaaag atggacgaac tagtgttatt tagtaataaa agcgaagcaa ttctttttaa
    22921 agcaattggc tttttagtcc atgttgtcaa agacgaagaa gaaattactg aaatcttaaa
    22981 agaattacca aaagaagtaa aagtaatagc ctatgacacc ttttttacat cgttctttac
    23041 tgagtacact aaaaatcaaa aacaattata tcccctttat ttaccgctcc catttaatac
    23101 ggaagatgaa ggtaaagcaa taagtgagat gaaagaaagt attagaaaat caattgggat
    23161 tgatcttcta taagaggaaa tgaatatggc aaaagtaact ggtaaaattg ttaaagtatc
    23221 tggtccgctt gtcgtagcaa gcgggatgaa aacagcgcaa gtttatgacg ttgtgcttgt
    23281 tggtgataaa caaattattg gcgaaatcat tgaattacgc gatgatttgg caagtattca
    23341 agtttatgaa gaaaccgctg gggttggtcc tggtgaacct gttaccttaa ctgaagaacc
    23401 actttccgtt gaacttggtc cagggttaat ggaagcaatt tacgacggta ttcaacgtcc
    23461 tcttacttca atctatgaat tacatggcac ttatattcct gttggtgtgc aagtaccaaa
    23521 acttgatcgt aaacgtaaat ggaactttat caagactgta aaagtcggcg aagaagtcga
    23581 agaaggtgat gaattaggtt atgttaatga aacacctgca gttaaacatt taattttagt
    23641 gccgcctgga gtaaaaggga aagttactaa aattaatgaa ggtgaattta ctcttgaaga
    23701 agtaattgct gagttagaaa caattgacgg caagaaagaa cttacgatgt tacaaaaatg
    23761 gccagtgcgt aaaaagcggc cttatcttac aaaaaaacca cctaaagaag cgatggttac
    23821 tggtcaaaga gtgattgata ccttgtttcc aatcgctagt ggggggattg cagcaattcc
    23881 tgggccattt ggtagtggta aaaccgttgt tcaacatcaa ctcgctaaat gggcggatgc
    23941 agatttaatt gtgtatattg gctgtggtga aagagggaat gaaatgactg atgtcttaat
    24001 ggaattcccg aagcttgttg accctaaaac aggattatca ctgatgaaca ggacggtatt
    24061 aatcgctaat acttcaaata tgccagttgc ggcacgtgaa gcaagtatgt ataccggtat
    24121 tacaattgcg gaatattttc gtgatatggg ttaccgtgtg gcgattatgg ccgactctac
    24181 ttctaggtgg gcggaagcac tgcgagaaat gagttcacgc cttgaagaaa tgccaggcga
    24241 agaaggctat cccgcttact tagcaagtcg cttaagtgac ttctatgaaa gagctggtgt
    24301 tgttgtgact ttaggtaaaa agaaacgttt aggttcgatt accgcaattg gcgcagtttc
    24361 gccgccaggc ggtgacacta gtgaaccggt ttcccaagca actttacgga ttgttaaagt
    24421 gttctggggg ttaagcgcta gtctagcgta taagcggcat ttcccggcca tcgactggtt
    24481 acggagttat tctttgtatg atgacgatat tgttaagcat catagtgcct ctttcccgta
    24541 ttggaaagat tatgtcgctc ttttaaaaga attattacaa gacgaagcag cgcttctaga
    24601 aattgttcgt cttgtgggta ttgattcact tagtcaaaaa gatcgagtca aactccacgc
    24661 tgcgcagatg attagagaag actatttaca ccaaaacgct ttccacgcgg tagatactta
    24721 tacttccatt gaaaagcaat acgggatgct tgatttaatt gttacttggt ttaaagaagc
    24781 aaaccttgca cttgacgaag gcgttgaata caagcaaatg gaaaaccttg cggttaatga
    24841 acgcataagt catataaaat atgtcgaaga aaaagatttt gataaacaag ttaaagcgct
    24901 taaaaaagat gttattgcgg catttaacaa cttgaaggag gaataaatat atggcaatta
    24961 aagcatttaa aacaattaaa gaagttgttg gccccctgat ggttgtcgaa aatgtcagcg
    25021 gtgttaaatt tgatgaactt gtgcgtatta ttcaaagtaa cggtgaagag cggcttggta
    25081 aagttttaga aattgataat gataaagccg ttgtccaact ttttgaaagt agtcaaggat
    25141 taaaaattga tgacgcgaaa gctatcttta ctggtgaagg tgttactttt ggggtatcac
    25201 cacacatttt aggaagaaag tttgatggtc ttggtcggcc aattgatggc aaagggcaca
    25261 ttattccttt taaacgcatt gatattaatg gtgtaccgct taacccagta gcgcgcgatt
    25321 atccaaacga atttattcaa acgggcatca gtgccataga tgggttaaat actcttgttc
    25381 gcggacaaaa gttaccaatt ttctctagtt caggtttaac acataacagg ctcgctgcgc
    25441 aaattgctaa gcaagcgaca gtgttaggac gcgacgaaaa atttgcggtt gtatttgccg
    25501 caattggcat tacttttgaa gaagcaaact tctttagtga agcatttgaa gaaagtgatg
    25561 cgcttgaccg gactgtaatg ttctttaatt tagcaaatga cccagcgatt gagcggattg
    25621 caacaccacg tatggcgatt accgcggcag aatatcttgc ttatgaactt gggatgcatg
    25681 tcttagttat tttaacggat attactaact acgctgaagc actgcgcgaa atcagtgctg
    25741 cgcgtaaaga agtgcctggt agaagagggt atcctggcta tatgtacaca gacttagcct
    25801 cgctctatga acgcgcgggt cgcgtaaagg gacttaaagg ttccattacc caaattccga
    25861 ttttaacgat gcctgaagat gataaaacgc acccaattcc agacttaacg ggttatatta
    25921 ccgaaggaca aattattttg tcccgtcccc tttcaatgaa aggcttaacg ccaccgattg
    25981 atgttttacc ttcactttca cggcttaaag ataaagggat tggtaaaggt aaaacgcgtg
    26041 aagatcacgc tgatacaatg aatcagctct ttagtgcgta tgcgcgcggt aaagatgcaa
    26101 aagaattatc acttatttta ggtgatagtg cactttctga aattgaccaa aaatacgcgg
    26161 cttttgccga tgcttttgaa agagaatacg tttcacaagg ctttgacact aaccgtagta
    26221 ttaaagaaac acttgatctt ggatggaaac ttttaggaat gttaccacgg caagaattac
    26281 ggcgtgttga tccaaaactc gttgataaat attataagga aagtaaagag taattaatga
    26341 gcactgcgca aattaatcca acccggatgg agttacaacg gttacgggat aaactagcga
    26401 ccacaacgcg tggtcacaag cttttaaaag acaaacaaga tgaattaatt catgagttta
    26461 ttccgctgct acctgagtac cgcgctctgc gggaaaaggt tgaaagtgca ttaagtgcaa
    26521 ctttaattcg ctatcaattt gcgtgtgtca aaatgagtaa agaagcaatt aatgaccata
    26581 ttaatgacag tgtaacgaaa catgaattaa gtgcaaagtt tgatcgcgta atgggtgtag
    26641 tcattcctaa cttagaaaca aaagctagtc acactgaagt taattatgat ttactatcga
    26701 caactagtgc ctttgatgat ttaatcacag cgattcaaga tttatttgcg ttactcatcg
    26761 aacttgctaa cttagaaaat accattaaaa tcttagtggc ggaaattgag aaatcaaaac
    26821 gccgtgttaa tgcgattgaa aatattgtca ttaaagaaat tgaagaacaa attcgtgtga
    26881 tacgcatgaa actaacagac ttagaacgca gcaatacaat tcggatgatg aagagtaaag
    26941 aaatcatcat ggaaaagaaa tttaacgaag gtaaataaaa aagtgtgatt gccacactta
    27001 attattaata tacgtacgcg ctatgtgtgc gtaaaatttt ttaattagtt ttaccattac
    27061 tgcgtttacg cttatctcta aacatccgct catcgataat gcgaataagt tcaccagtgc
    27121 taatttttaa atctttgtta tggataaagt atcccgcgct gaagactagt ttaaaattac
    27181 cgactttatt accttcatat tcactaaatt gttcgcgaat gaccgcaaca cgttttttaa
    27241 gttcagcggg cgacatacta tcaagaacga taataaattc gtcaccgcca aagcgtgcga
    27301 caaagtcaac attgtcacaa tgatggcgta agaaccgcgc aaattgacga agagcgtcgt
    27361 ccccggcatc gtgacccaat gtatcattaa tcattttaaa attatcaagg tcaaacataa
    27421 taatggaaaa actgctcgtt ttccccgtac cagtcatgcg actatgaagg tggttactta
    27481 agctccgacg attagcaaga ccagtcaaga aatcagtaga agcatgaata tgttgaacat
    27541 tcattgccag aatcatatag ttaaatatat tattcataac cataaacgga ataaaattat
    27601 ttaataagtg accaacaagt gctaatatca gagtagttat aataagtaag aaagtaaagc
    27661 gctcactttt cccaaatgtt ttaaagaaac gcgtgtaaaa acaaatggtg agcgctaaga
    27721 ataaaaggaa aataattgtt tggacaaaga ataatttacc tctagcatat acgccatcaa
    27781 caatagtaaa gtaaatatta gtgccaggaa ttaaagatat aagtgaaagc gctaaattta
    27841 aaagtaaggt cgcagcaaca acaatgttat aagcgcgcgg tatctctttt aaatatgaag
    27901 tgtgactaat atataagcac cataatcccc acatgactgg taagcaaaca tatgtagcgg
    27961 taataactcc agcaaaggcg tatgacggaa gattaattag tcccccataa tctaaacgat
    28021 aaagtgtatc aagaattatt cctaagattg taaacacata aagtaagacg acaacccgaa
    28081 agcgacgtga attttgatca tcttgccgat ttaaaaatat aaaagacgct gtcatgattg
    28141 acaggtagaa gaatgcgcta acaataacaa agaaattctc catagatata ttccagtgtg
    28201 ttgtattata tctacatata ataatttttg aaaagtttta aacttaaatt tactgatgta
    28261 agtgctttta ttttcgtcat agaatgcatt aaatttaaaa ttgttctcga aaaatctaac
    28321 aaaaatttca attgtaaaat tttactttaa aaataagaaa gataacgata acaaatgtaa
    28381 ataaaatata taaacaacct tttaaaccac catagaatga taaaaataaa tggaaaaacg
    28441 aaaaaaagtt aaaaaatttt atattccttg ttgacaaaat ttctgaatta tatataatca
    28501 tggtgtaaaa ggcaaagcta gagcgatcta gcgacgcaaa actatagggt cttagcaata
    28561 agatagccag ctaccgagcg taagaatagg tggctgtttt tattactcgg acttaaaact
    28621 agtaatacat tacaaagaac ttactcttcc gactcggtca aaccttgtaa gacacacgac
    28681 aagcgcaacg aaaggaagat gaacatatga aaaacaaaaa atcaattatg tttggtgctc
    28741 tagcgcttgt tttacttagt ggtctttccg cagcaagtac aacattcgcg tggtttacca
    28801 cgacacgtac tgcttcactt acttttagtg atgcgactgt tgtatgtagc gatagtaatc
    28861 tcgttgtgac ttacgttgat tcactaaaca ctttcggaac gattagtgac gaagcaaacg
    28921 aaattaaatt aacgggtgcg aatcgtgtca cagatatttc cggaaatggt ttagattttt
    28981 acaaacccgt gtggtcgcca attgaaggcg ttgctacaaa tattaaagaa gtagcgactg
    29041 ccgaaaatta ctacatcgac ttcgtcctta agattgctag acaaagcgat tccccaagcg
    29101 caacgggaat taaagtctac ttaggcgaaa atacgaagat tcttccggtc gatgccacta
    29161 aaccacgcga tgttgatgca gtaagcgcag tacgcatggc agtaataagt tatgcggacg
    29221 aattccgcgc ttcgcctgca ctaattgaac tgcacgcccc tgtcgtcgaa actagcccag
    29281 aatatattat taaagatacg gctagcaccg cctacagtgt taatggctat aaagttcatg
    29341 acgcaagtgc agaagttaac agtgatccat tcgttacaaa acttagtgaa gcggatggac
    29401 ccactcatca aattgccgat ttatcgagtg actccgaagc atacgttggg tttcgcttct
    29461 ggattgaagg tacggacgcg gaagcgacca aagacaaggc actcggaggc atgttcaaag
    29521 ttgccctaga catctatagt ctctaggcaa gtactacact aatatcaaag atattaaatt
    29581 agaacctaat tgcccaatta gttcatctca tcgatgaaag tggggccggc cacaacttcc
    29641 ggcaattgtt caaaggagga tattacatat tatgaacaaa aagaataaag tattattagg
    29701 agcgatcggc ttagttctct taagtggaat cgccgcgaca agctcaacct ttgcatggtt
    29761 tacaaccgtg cgtaccgcta gcatctctta cagcgaagct acagttcaag cttcagatgg
    29821 caacttagcc attgaatacc taagctcatt aaataattgg agtggtggtt caacttatga
    29881 tgctgaagcc aataaagtta caattgctgg tgctaacaaa gttactgaca tttctggtga
    29941 tggtaaaaca ttctataaac cagtatggac aaccccagtt agtactgctt caagcatcga
    30001 aacagttcca accactggtt ctcctggtgt tgctgatgga ttctacattg acttcaccgt
    30061 ccgcttaagc cgtgataatg atgttgatga aaacggttta aaagtttacc ttggtactgg
    30121 taccactatt gcgccaaaag atcctgaaaa tcttgcagat actggtgcag tcaaagctgc
    30181 tcgtttagca gtcattgatc atgaaggaga cgttaaagtt cgctgggctc cagaacaaga
    30241 aacagaacca aaatacatta ctactggtac tgactcactt tacggtgtgt caggtttcga
    30301 aactacaact gatcttaact ataaacacgg agaaatcact tctgcagaca gcaaggccgc
    30361 tgctgatctg gctggtttct tagttgctga cttaagtaaa ggtgttgcta ccgaagcaaa
    30421 cgttaccttc cgtatttggt tagaaggtga agataaagac tgtaataacg acattctcgg
    30481 tggtgtgttc aatgttaaca ttgctttata cacattcatt aactaattaa agttgcgacc
    30541 ccgtttcgcc ccagggacat tctctctggg gtgaaacttt ttacaaacct caataaaaaa
    30601 ttaaggaaat aaaactaatg aataaaagaa ataaaagatt aggaacagta ttaacgctat
    30661 tatttattag cgctattgcc gcaactagtg gaaccttagc gtggtttaca actgtgcgta
    30721 ctgcaggtat cacttatggt gatgctgaag tttacatgcg ggattctgat ttagtggtta
    30781 cctataagag ctctttgaat tcattatccg ctactagtga actagacgga atcactaaaa
    30841 taacattaac cggcacgaat aaagttacag atatctcggg caatggtatc aacttctata
    30901 aaccgcgctg gaattcagac aatgtaaccg cagcagtcat taataccgta ccaagtacaa
    30961 atcctggcga tgcggatgga tattttattg atttcactct tacattatct cgctcgcctg
    31021 gcgaagaccc attaaattta tatgtctttc ttgggcaaaa cacagcgatt ctgccggcgg
    31081 atccggctaa tccaaaagat gttggtgcat taaaagcatt acgcatggcg gtaataagtt
    31141 atgataacaa tgactcagct acaggcacgc caagtgttga tatcttatat gcaccagaag
    31201 cagaaataaa tcccgaatat cttcaattag gaagtggggg agcgtatggt cttactaccc
    31261 atgaattagt gcccgcaaca gtaaaaagtg attcgtttga gacacactac actattgcag
    31321 attcacaagc agtttatcca gaaattgcag tgttaccagc aggtactggt accgctgatg
    31381 tgacattccg tgtgtggatt gaaggtactg atattcatgc tgctgaaagc attattggcg
    31441 gtaagtttaa aattaaactt gatatttact cgacaggagt ataagctctt aatattaatc
    31501 atgaattatt aaaacacacc atccagttgg gttgatgttt tttattaaaa aacacgtatt
    31561 tttataaaga tttatcgtta aagagccata taatcgcata gatatgtttg acacttaatt
    31621 acatttaatg taaaatttta ttacgtgtga aataatacat gtgaaggagt acctcatgac
    31681 tggccaagaa aagaaaaaga aatttaaatt accaagttgg gcaacatatt tgctacttgg
    31741 tttagaatta gcgctgatgg tcgctttagt aaccatttct attgtcgctt taaagaatgt
    31801 tcaagcagga tcaggaggcc catttattaa atggttaatt ggtaaccgcg tatggttttt
    31861 cgtcctaatt gtccttccgc ttattgtgtt atttctcttc aatatttatc tcttaattaa
    31921 agtgatgaat gaagcttcaa gtaaagaatt caaacaaatg acaaaagatg aattacttga
    31981 agaagcgcgc aaacaagcgc ttgaagaact ccgtaaagag ttagcaaaac aaaacgaacc
    32041 cgctcctgaa acgaaacctg aagaataatt aatcacaaaa tacttaaaat tgccgtagaa
    32101 aatacggctt ttttaatact taaattagat agttcttgac acttttatat gtacaacata
    32161 taataactac agaaaaggca aacttagaga aatctaagga cgcaaaacta tagggcctaa
    32221 aagtgtgatg gtagccagtt gcctcgaaga aataaagatg aatgttatta tttctggcaa
    32281 agagtggtta gcatcatttt tatttaatcg ccactatcaa attataaaat taggatagtg
    32341 tgcacggaca ttcgtgttaa aataataaag aatgttcata tatcactaaa acatcattga
    32401 ttgttttagc ggtataagaa aacggagaat attgtggcag aagaaattaa tttaagtgaa
    32461 catccacttc ttgaagttgc taacgacatt attcgtgaag aaaagcggcg gaaacgcact
    32521 aaaaaagttg tcgctgtttc agcgttcatc attattgttt ttatcattat ttcatactta
    32581 gggtatgcaa ttggttacta tattgcaaac gcaacctcgc tttccttatt gtcaaaagac
    32641 ccagaagctc gctttgcgca agttgggttt agacttagtg aaccgctaac agttacagaa
    32701 caacataaat acaatttaca gtctgatgaa ggttgggttg gtggtgaagg ttatgtgtat
    32761 tggacaacgc aaaacgtgtc gccacagacg atgagttttg tgcttgagca tgagggctat
    32821 tcaaatacaa atagacttag cccagttact tctggttctt ttacgaaaga tgaagaccca
    32881 tatttaccac ttacgcttta tgaagcaccg aagcaaaacc gtgcagaacg ccacaatttg
    32941 aacttaaatg tagcagatcc aaaagattat tttgggttta cagtattatt tagactttta
    33001 attgacgaag atcgagccgg aacaatcccg cttgataata tagccattta cttcaataga
    33061 gacacgaagg tccatggtca aaatgaatta ccaaaagcat tgcgttttgg tttccaaagt
    33121 ggagctaaaa aagacatcat tagtccaggg cgcactttat tagaagaaaa tgattataca
    33181 ggttcaattg ctgttggtgg cagacttgac ttagatgacc cgcgtgctcc agcgtcactt
    33241 acaggatttt atgattatac atttggtctt gatggggcga agtatggcga aagtggtgaa
    33301 caaatttatg aaatagctta tggcgaattt gaacagccac tcacaaatga aaactggggt
    33361 gaaccacttg cggaagcaga agaaaagtta gaacatggtg atgttttcca tgcggtgact
    33421 tacaagggag tccgtccgct tattaacgtt gtaccacagc gccaatatta cacaagtttc
    33481 caaaaatata tgcttgatgg tgataaaggt gaaccgcttt gctacacaaa taaccttggt
    33541 gtggcagaaa tgaatattaa attttggtta gaaggctggg acttagcgac cacaaataaa
    33601 gttgtaaaca agtcttttgg aatgcaatta cactttgtcg ctaaaagggc tgaataagaa
    33661 gaggaaatgg tgatattatg aacgcatttt ttagttatat ccgagacaca tttgaatcat
    33721 tctgggctaa ctttgtcgaa tggttagccg ctgtatttgt tgaaccatgg aaactagtgt
    33781 ccgatgactt tggtacttat cgtacaatca ttgaaatgta catggactca tttggatttt
    33841 ggggttggtt tttctttgta atcttaatga ttcttaatgt ggccgttatt ggtggcttaa
    33901 tctttttatt agtgctcctt attcgtcgac ttgttaagta ttcaagcaaa aattatgata
    33961 aagcgaaact tattgccgaa gtacaacggc ttaacaaaga actcttcttt gccattcaag
    34021 agaaaaatga agtacttaaa ctgaaagctg aatcattagg tcttgaagat ccttcacttg
    34081 tggaaggtga caaaacttcg gtcgttattg atacattccc aaaacttggg gcagtggact
    34141 taaaatataa agataaagac actgatttta ttacaccggt attagaccgc gaaattactt
    34201 tagaacaaat tgcagtgcgc tatcgttatt ttgccgcatc gcaacttggg ttattttatg
    34261 atatcgactt agtgcgtgtg ctctttgccg caatgggtac ggttaaaatt attattttag
    34321 aaggtatctc tggtactggt aaaacttctt taccttactc cttaggtaaa ttcttcttaa
    34381 atgacgtcgc gatttgtagt gtgcaaccgt cctggcgtga ccgtacggaa ctcattggtt
    34441 actataacga atttacgaag aagtttaacg aaagtgaatt cttgcgggcg atttacgaag
    34501 caagttatcg taaagatgta aacattgtta ttcttgacga aatgaactta gcgagaattg
    34561 aatactactt tgcggaattc ttatcgatta tggaaatgcc taacccacaa gagtggtatg
    34621 ttgaagtaac aaagacagca gatgaaatga acccagtgca attagtgaat ggtaagttat
    34681 taattgggca aaatatttgg ttctttggta ccgcaaataa tgacgactca acatttacga
    34741 ttaccgataa agtctatgac cgtgcgatta gtattacatt aaacgaacgt ggacaaatgt
    34801 ttgatgctga atttactgaa ccattaaaga ttccgtatga acacttacgg cggttatttg
    34861 atgaagcaca aactaatttc ccactttcca aagcaaacgc taataagttt agtaagcttg
    34921 acgaatatgt tgtgaataaa ttccgcattg gatttgggaa ccggatttta cggcaaattc
    34981 atacatttgt accaatttat gtcgctagcg gcgggactga accagaagca cttgacttta
    35041 tgttttcatc caaaatttta aagaaattta gatcacttaa cttagcgttt atgcgtgacg
    35101 aattaagtgc gttacgcgcc tttattgaac ggacatttgg taaaaacaca tttaaacatt
    35161 cacttaatca aatcgatgat tttatgacga gagcgtaggt aaataatggc agacgagaat
    35221 aaagttacaa ttaaacaaga acaaaatgtt gtagcggaag aagtcgctaa ggaagaaact
    35281 gcgcctaaaa caaaaaagga caaaccttta aaagtggctg gagcccaaga aaaagtaaaa
    35341 aaagcgccaa ttaaagataa agcaaaaaaa gcagcggaca ccaaaccaaa acaaactaaa
    35401 aaagttgcta gtaaaaaaag tgacactaaa gttgaaaagg aaaaagtgga cgctaaacct
    35461 aaagctgaaa aggcgaaaaa agcaaaggct gaccctaaaa aagctgacgc taagcctgaa
    35521 actgagaagg tagcggaaaa agcacttgat actgaagcac ctgccgctaa agaaaaagaa
    35581 ccgaaagaag cgcccaaaaa agaagcacca aaaaaagctg tgccacgccg tcgacgactt
    35641 tcggcgatgg aattaagaaa gcgtgaagca ttaaaaggct acaagcgttt tactaaggtt
    35701 cgtagccgtt attattcatt tttaaatcgg actgttaagc aaagtaagcc aacgacgaat
    35761 ttatacaata cgattgctaa taaagcacca actttgtctg gtattacgcg taatgaagta
    35821 aaacgttttg acccagagtt tattgaaaaa attgaacgcg tgattccttc tcttgaagaa
    35881 attgtcgtta atccgcataa atttattaat gaatttgccg aagtcgtgca agtagaaaaa
    35941 gcgcggaaac ttaccccgcg agcagtcaag tatatggcgc aaaatgctga tcatgtatct
    36001 gaggtattag atgatggtcg tgttatcccc aaaaagattc ttaacgttta tgttgatgac
    36061 gacttaaaga tttatgagaa ccgtttcatt atgacactaa ttaatcggtt acaaatcttt
    36121 attgaattgc ggcataaata tattagagaa catggtgata cgcgtaatag tgaccaaatt
    36181 acgattaagc gtgaagtaca agttgaagat tcgctcttca cagttgatgc aacaattaca
    36241 atgtcagtgc ctagtgatga cgaaggggca cgcgaaagta atatggattt acttgaacgt
    36301 ttagaaaata ttagaagacg gacacaattc ttagttaata gccgctttat gcgtgaaatg
    36361 gctaaagccg tacctgtgtc tgagccaatt gcacaaacaa atattattcg tttaaactgg
    36421 gcgtatcaag atgcttttaa attatggaac tttattcatc gctatgaaga actaggtatt
    36481 tcatatacaa caacccaagc caaaattgaa tttgataaaa aatatttaga taagattaat
    36541 caattttcat taaattcatt ctttacaatt gaaacagagc atgcgcgcct tgctttaagt
    36601 aatgttgagc aaaagaatat tcgtccgatt attgaagcgg gcgacttaaa ttatgaatta
    36661 agtgatgaac gttttgctaa accaggatta ccaatgcgtg ttgacgctcg tgttgaaact
    36721 gctgaacaag aagcagcgcg actaaaacgt gaagctactc gcgaaaaagc tagacttaaa
    36781 cgcgaagaag caaaacgtaa acaaaaagaa cgtgaagcct taaagaaagc gcgggcaaaa
    36841 gaaagagcgc ttaaacgcaa gttagaagct gctgaacgcg ctaaacggcg caaagaagaa
    36901 gcaaaacaac ggcaaattga aaagaatatg gaacgggcac gcttacgcgc ggagaaagaa
    36961 cgcttacttg ccgaacggaa agcatatcaa aaattaatga tgacggaagc agaacgctta
    37021 cgccgagcac gtaaggctgt tgccgcacaa gcaactgaag aaagaaagaa ggcgattgaa
    37081 gatgccaaag cacaagcaaa atagtgaagc agcgccaaag aaaaaagggc gtattggtaa
    37141 aattacaagc gttgttttag gggcgatcgt tgttttactt attgttttac aagtcgttgg
    37201 tcaatcaacc gctaaaaata atcatggagt gatgcgttac ggtaattatc agtcattacg
    37261 cgttttaact gattcaatgg aaccaacata caaagttgat acaatggtat ttgtaaaacg
    37321 tgttgatcca aaaacactaa aaggaccatc aagcgaagga gcattagatg gggacgtcat
    37381 tacttttgtg cgtaattatc acggaagtta tgatttacct ggtgataaac gcattgtcac
    37441 tcaccggatt catcgtattg aggtgcaagc agatggccgc gtttacttcc ggacgcttgg
    37501 tgataatatt cacgctaaag agtgtccaga aagtggttgt acattagaag cgcgtggtga
    37561 ctgggtgcgt gacacggata ttttaggcaa agttgttggt caaagtatgg tacttggtaa
    37621 atttagtgtg ttgctcgcta atccaatatt tatgcttgtg tttgcgatgg taccactctt
    37681 aattgtgttt ggttcttcac ttatggacct cctaaaacaa ttaaaaagta ccgaggggag
    37741 tgaagccacg ccgagtggca taagtgaaca acaggccgaa tttgaaagaa ttaaagaaca
    37801 agagaaatta cgattattaa ttgaattaga aaaagaaaaa ttacgaaaag aattagagga
    37861 gggcataaaa gatgaaacaa aagaaagcta gaagacatag cggaaaaaag tatttatggt
    37921 atagcatctt atgtgtaatg cttttagtgc ttgcttttag tacttatgtt ttaatgtcct
    37981 tcggtcttat tcctatgaca gggaacaaaa cacttgatat tataatcggc attttattag
    38041 taattataag tatcgctagt attgtatgtg ggattattgc ttttgtatat tataagcgaa
    38101 actcaaacca aattcgcttt aattatgcga catttgaatt agtggaaggg attaattcag
    38161 aacgagcgtt tttagcctat gtcgctaaag atattaagcg taatagaggt aaagcaaatg
    38221 cacttgtcgc ttttgcggca acacgtttta aaaagattgt ctttacgcga tatgggtatg
    38281 agaaaggcgg cgaagtttta ggtttagtgt atgaaacatt taatacactt aaaacaaaat
    38341 acccgcattc ttattacggt tacgactata atgaaaactt ccttatttat ttaagagata
    38401 caccttatga acaagtacaa gaatatgtga atgacttaat tgaagaaatt aataaaacat
    38461 tatttgaaaa tcgtaatgag gtaaaatttg tggcggactt tggtgtgagt tactgtcaaa
    38521 atgaaaatga cgaatttatt accgcggcgt cgatgctcca acgggcactc attgcagctg
    38581 atcacggtcg tgccgaaagt gatttaggtg gtttaacact ttatgaacca aagatgtatg
    38641 aacgtaataa acgaaatgtc caacttggtt tacaaattga acgcagtttg gaattagaac
    38701 aattcgaact gcactatcaa ccaaaatatg acttaaaatt aggccgcttt gcgggcgctg
    38761 aagcacttgt tagatggcgc catccagagt taggactact ctcacctgca aactttatct
    38821 tatttgccga acaaagtgac ttaattatta aaattgacca ttatgtgctg gaaaaagtgt
    38881 gtaaacaact tgatgaatgg cggggtaaag gacaacgctt attaccaatc agtgtcaaca
    38941 tttcaaaacg aacactcttt actgggaata ttattaatca cctaattaaa gtaacagata
    39001 aatataatat taacccgatg ttgcttgaaa ttgaaatcgt tgaatcacca tcactgcaag
    39061 acatattact tttactctca attgtgaaga aatttaaaaa cttgaaagtt aaaattgcca
    39121 ttgacgactt tgggactggt tactcttcgc ttagttatat taaaaagatt ccctttgatg
    39181 ttattaaaat tgataaggct ttccttgatg acgttgatat tgatcaaaaa tcacgattaa
    39241 tgattaaaaa cattattgat ttagcgcaca ttctcgacgc ctatgtcgtt atggaaggtg
    39301 tacaagatga aagacaagtg aagatgcttg aggagatggg ggctgactcc atccaagggt
    39361 tctactatag tagaccacta ccacctgaag aatatcaagc tttcctcgaa gataacctct
    39421 tcgaagctaa taagaagaaa aagcgaggac ggaaataatg attttactat tgggaacaac
    39481 tccggatgac attctctata ttaaaaaccg gatgattatg aaagaaaaag gtgcggttaa
    39541 taaagacgaa cacttttact ttgtcgggga atatggtggt aaagaaatat gtatgactta
    39601 cactgggcat agtaatatta tgtcggcagt gctcacaagt tacatgattc gtaaatttaa
    39661 tccttatctt gttatttcaa ttggcagtgc acaaagtgca agtagtaatc ttaatcaagg
    39721 cgatcttttt attgctgaac gcgtttatat cgcggactta gattataaca attacacaaa
    39781 tttaccgctc agagacgcaa ttcatatgca cccatattat gttagtgaag aagtgtatat
    39841 taaacacttt gaactattaa acagtgcaac agggaactat aaattagtaa ggggtcccgt
    39901 catcagtgtt aataactttg tgaaagaaaa ggcggaagct gaacgcatta ttgcccgtca
    39961 agaaaactat attgaagggc caacagtgtt tgatacggaa atgggcggga ttgtagcgtg
    40021 ttgtcggttc tacgggatac cgtggcttta tcttcgctca attaattacg aaattggtaa
    40081 agaagaacaa ctcatttcct ttgtgcgtaa aggtgttgaa gcacaaccac gcatcggtga
    40141 gctagttgaa caactattta tattcttaaa tacttcgctt gaagaaagta tttaaaaaga
    40201 aaggagtaag tcatgattgg cattaatcac catgaaattg aaaatgaagt aacagcgact
    40261 ttgcacgcga tttcagttga taatgatttt gaatttcaat taccacttga cgttaacgct
    40321 aaaacacttg gtgttacttt aggtgtacca gtcgtgatta gtctttataa cgaggctttc
    40381 tattatcaaa ttacgattag tgattatcgc cgctctaatt tcatgcaagt gagtatgcga
    40441 cttttcattg gcgaaattcc taatccacaa aagttatatg cgcttattaa taaattaaac
    40501 actaaattac tctatgccaa agttgtttta gatgaagagg aagataacac attaatttta
    40561 gaacacgact ttaccgctgc aagtgtggaa caattaagtg aatcgattta tgactttctc
    40621 gttgatgttg tcgatgaaga tgtttcacca ttaatcgaag aattattaaa agaaattcgt
    40681 gattaaaaca ctataaaaat attcgaaaag ttcaaaaacg tgatattttg ccatttaaaa
    40741 agttcaaaaa cgtgatttaa cacttatttt tattattgtt ggacattttt attgctttta
    40801 tttttattta ttagatacat gattaacagc attaacattg ttgcacataa gtaccctgca
    40861 ctatcgataa taacatcagt aatagtacct gctcgtcctg gaacaaaaag ctgaataaat
    40921 tcactaagag cagcaataaa aacaccagta ataagagaaa gcactgttat taacctagtg
    40981 ctttttttat cttttaaaaa taagtaaaaa gtgaaaaatc caaagatccc gtcgataaaa
    41041 aataagaaga agtgaccaac tgcttttcga acaaagcctt ggaagtcact aaaattatca
    41101 tcattaataa agggtcgcgg aataatttta atactaaaag atgcgctgat agtagggtcg
    41161 gcaacacttg tcgcgctaat agtaacaacg ccttctttta aagcaacaat agtgccaaac
    41221 atgttcccag ttgatacgcg cgcaatactg ttatcacttg aagtaaaaat aacacgctta
    41281 tcagtcacac ttaacgattc tttcgctaag gaatgattta agtcataacc gacatttacc
    41341 cgctgaccag taattaattc agttggaaca ttagtaaaat taacagaagt tggcatcaca
    41401 ttattaacga taatatcttt tgtggccctg attgcaaagt tactatttaa gtaagcagta
    41461 attgtagcgg taccttttat tttaggtgtc ttaataaagt aattatcatc ttctttaata
    41521 acagtagcaa caatgtcatt acttgataca aaagttagtg atggatcagt aacattttcc
    41581 acgtcaaatg tataatttag ggggattttg tcgtaaacat aagcgatttc atcaaattct
    41641 aaagtaattg aagttggcga tattagcggt gtatcattac tcttcacact caccttaaat
    41701 tttcggtcgt caccaacaac accaacataa gtttcgccaa cgctaatccc tttaataaca
    41761 ccgtacttat taatagttgc aacactttcg tcttcactaa agaaagtgag tttacgaaca
    41821 tcatattcat tagcgacact ttcatcaaca ctaacttcaa gttgtgtagt agtgtcaata
    41881 gtaatcctat ctttagtaag agcgacttcg tattcagggg gcgcaacttt ttcgtgaata
    41941 gtgacataaa aactaatgcg atgcagcgga ctattagtca tagcggtgac taatacattt
    42001 tcacttagtg cgcgcgcttc taagtaacca cccggataaa cttcaagcgc actactatca
    42061 ctacttgtcc aatttacccc tttatccgta gcattttctg gtaatacagt tgcagtgatg
    42121 cgtctagtga tgcctagcgg tatatagaag tgcatacctt cagttaaagt agtgtcatct
    42181 ttagttttta gtgttaatga ttcaatagga acataagctg tttcaggacc gtccactgtt
    42241 ttattaacaa acacacttaa aataagcgag agtaaacttg agcgcgctcc agaagtacca
    42301 cccgcgagtg ttgcttcaaa aataataagc ccgtttgtta ataaaaatag gctcaagata
    42361 aagtaacgaa tatttctttt aagccagttc atgtttatat tatagcaatt agattgatta
    42421 aaaatagtcg catactacaa ctaaagttgt aatctaacat gttttagtta ataataaatg
    42481 acagacttgt cataaagagg ttagatcatg aaaaaatgtc aattaatatt attaccatta
    42541 ttaactatgc ttttaagcgg ctgtacgcct ttaagtttaa gtgaagtatt aaagccttct
    42601 gataactccc taagtgaaac agatgtaccg tcaactgaaa gtagtagcga aatagaagga
    42661 gcacaaactt ggtcagtgga tgatgacttt acttggcaaa catatgaata cgctcttgat
    42721 agttataatg tgcctctata tgttccaaca gaaaatccgt atatagatat tataagtgac
    42781 gctgataaag aaacatttta cacaaacaat tataaaagag catcatctta cgaagacgca
    42841 atgtttcgga cagaaaaagg tttaatctct ggcgatataa ttgacactcc aaacacggca
    42901 aactatccgc ttaaccacct tcctaatcga gtatatcgca ctctagcgaa ttaccggatt
    42961 aatgaaggag tgtatgaata tacattagat ggtgaatata agtcatacac aattaacaat
    43021 attaatggca aagtaaagaa agtctattac ggggctgctt atgtttcgct tgatgatgtt
    43081 gcggcttatt tatttgcttt tggtgaagca cctgcgaatc aaatgatggc caaaaatagt
    43141 actgcaatga atcaagcaat atccttattt ggtaaatatg gacgcgttaa taacggttat
    43201 tacagcagtg atgttactaa atataaatat gaaccttctt taccgcacac tgataatggt
    43261 ggaccggttg aagcggatat gtataattat cgcgaaatgg attttggtta cacttatacg
    43321 ccgtggggat atgggattga aagtgacgat ccatataata acggcactaa aattacacgt
    43381 agcactgtac gctttgttta tactgcatat gataacgagg ggaacgaagg cgctaaatat
    43441 attccagttg aacatcgaca tgtgttctta acaattaatc actataatga cttctttgaa
    43501 tatttaaatt atgaaggcgg atggagcgag tcatttggtt ggatgagcgc cggtaatgaa
    43561 tattgcgctg gtatgaactc gagtaaatac ggtaaaggat attacgattt tgaaaatcct
    43621 gtgccaccca caaaatacaa tattcctcaa gtaaaaacac ttactgaatt acagtcgatt
    43681 ttcaattgct tatagtcttt acaatatgta catatgaata gcaaagccaa aggcaaagat
    43741 gctcaaggct aaatatataa aaagcagtgt attataaagt tttcgatatt tattttcttt
    43801 tgaaataaag taataataac ttaaagaaaa gatgtcaatt atgattagta ttttgtgttt
    43861 tagatcgttt ttgcttggaa acactgacaa aacacgcacg aaaagtgtaa caggaataaa
    43921 agtaaagaca atgatataaa aagatagttg taaaaaccca aagaataact caaccattac
    43981 ttttctcctt tggtaaaaat agtgaaacaa ctggcatcac aaatactaag agcgtccata
    44041 atgttcttgt taacataaat aaaataacaa agagaacaaa agcgttaatt aaaagcatta
    44101 agaatcgttt aatattcata gcttaaatcc tcatatacaa catatgtctt aaacatttta
    44161 gactcatcaa tttcgatgat gcgcgctccg cgtctaagtg tgccgtagcc attatagcca
    44221 cttatttgcc cgtatcctaa aataattcca tctttttcaa aagtgaaatt attaagatga
    44281 tcatgtccag caaagaaggc ttcactaaca gtactttcaa ccattgcatt aaagaaacct
    44341 gtatcaactt cctgatgact aatacgctcg ccaataatgc ctttaacctt atctttattt
    44401 tctattaata atgcgtcagc gtattgtatt aaagggatat gggcaaatac tgtcgatttt
    44461 atgttattta ttttatcaat cataacttta ttacgatacc aatcaacctg tgcttcacta
    44521 aagtaatcat aaacaaaaag tctacctgaa ccagggattt ttagttctgc tttagaatca
    44581 agtaagtaca gattataaaa aggattatct tcatataagt aattaatagt aaagttacca
    44641 taaccaccat tttctaaaag gggaccaact ttaaaataca tatactttgt attcatagta
    44701 aaaatcactt ttagtaaccg ctccatttta tgatagtctg cttcatgatt accaaataca
    44761 aaagtccacg gtgttttaag tgactccata tgacgaatta attgtcggtg tctagcggga
    44821 gcagtaattg aaagtgtttg gtcgccagat aaaacaatta agtctggttt tgtttccgcg
    44881 gtaatattac taattaattt taatgtcttc cgatcattag cgtcaaatcc gaacgcgaga
    44941 tggaggtcgg taaactgcgc tatttttaaa gattcttttt gtaaatgaaa aacaaaagga
    45001 tcatcattat gatctaaaac gagtggtttt tgtcgcgaaa aaggtagaag tagcgctatt
    45061 aaaagtatta aaacactact aatccttaat ttcataaaag caccttgtta ctatttaatt
    45121 tatattcaag cgcggttatt tcataaactt taataaatct tttccctagt ttaggtaatt
    45181 cttctggtct agtttcaaca tagcgcatac cgattttttc cataatgcgg gcactagcga
    45241 tattttcatt atcacaggtg gcaagaatgc ttttaacttt aagtgtattg aagccaaacg
    45301 ctaatagagc tctgcctgtt tccgtgccat aaccttgatt ccaataggta tcatcaaata
    45361 cccaccctag cgttggtggt tttgggtatt catcaaaata aagaccacaa gcgccaataa
    45421 cggtgccgtc ttttaatgta acaataaaat caaaaacatc acgcggggtt tcactatttc
    45481 gactaacaac tgaagataca aatcgtttgg tgtcttcata tttatttggt ccccacatca
    45541 tatagcgagt aacactttct ttacttgaat atttttgcac agcatcaaca tcgtttagtg
    45601 taaaaggtct taagattagt cgtttagtcg ctaatttcat gatgttacct ccgtataatg
    45661 ttaattatat caatacttta ttatttaaaa ttgattttaa atgcaacagc aagtaaacat
    45721 taataagaaa aagtgttgca aagcttagta gagatgataa aatgtagacg cagaatagcg
    45781 atgaaacaaa aaaagattaa taatttaatt atagattaca tatttaataa ctatgataaa
    45841 gagacaccaa tttttactaa agatatatat caaaaatata gtgaaattag tagaagtaca
    45901 atccgctcta ttttaagtcg tttagtaaag gaaaataata tcgttagaat tgcagaaggt
    45961 atttactctt taccaaatta tgattgtgct atcggtacca tagctatttc caataatgaa
    46021 attattaata aaaaatatat tcgaaacgat ggaaagatat tcggttatcg tacgggcata
    46081 agtttcgcta acaaattagg gttaacaacg caaactgcag gagttgattc aataatctca
    46141 aattgtgtcg ctaataaaaa gcgtgaagtg aaattaaata aaacacgtgt tattgttaat
    46201 agtccgcgtg tagaagtcac agcaaacaat tataaattat tacagatcct tgacctttta
    46261 aatgattaca ctagttttag cgagataaat ttagaagaag gggcaagcac catttttaaa
    46321 tatttagaag gtgttaattt agaaagaaaa gaagtagaaa aaatagttaa tagctacccg
    46381 cgtgaagcac aagttaattt ttataaaatt aggtgtcagg atgctattac atcaaaatat
    46441 taaagagttt agaaaaattg ttattctaac cgctactcat tatgggctcc aagaatatca
    46501 aatagaaaaa gattattaca tatcattatt tttaaaaccg cttggtaata tgagtaaaaa
    46561 catccctata gtttttaaag gtggtacctc tttatctaaa tgttatggag ttattagtcg
    46621 tttttctgaa gatattgacc tttctgtaat tcaaaacaaa caactaacca gaaaacaaaa
    46681 aaaggatctt aaacaattaa taatagaaac agcaaaagaa attggtgcag aagtaataaa
    46741 cataaatgaa attgaatcaa gaaaacattt taatcgctac attctaaaat ttaacagtgt
    46801 ctttaacagt gatgtcgata cacttcaaaa acttattatt gaaacaatga ttgcctataa
    46861 tccatttcct gcgatttacc aaaaaacaga aaacttaatt ttagaatacc ttaaaattca
    46921 gaaaaaaaac gatataattg gacaatatca acttgattct tttttatttt taacgcaatc
    46981 tattgaaaga acttttgttg ataaaatatt tgcgttatgt gattatcatt tacttaataa
    47041 atatgagcga aattcaagac atctctatga tcttcatatg atatgggaaa gtggattatt
    47101 aaaaaaaaaa tacttccatc acttattgat aatgtaatag cggaaagaca aaaataccct
    47161 gaatacaatc catctgttag cgatggtcaa aaaccacgcc agctattaat gaatataatt
    47221 gattccgatg tatataaaac agattttaat aaagtgacaa caaaactgtt gtttcaaaaa
    47281 acaacttatg aaacatgtaa gaatacgctt taccaaataa tactttcaga attggtcccc
    47341 gaaatcataa ataaataagt gctataatta aactaataca tgaaaggaaa cattctttat
    47401 gaaatttaaa aagttattat tgctcccgtt agcggcatta ttacttaatg gctgcgcaat
    47461 tggtaaaaaa gttgataaag atgtcgcaaa tgagcgtgtg tcagaagcaa tacaaacaca
    47521 agaagaaatg gatgtagaca gtctctttca agaagcaaag caagatatga ctgaaagtgt
    47581 ttcacaaact aacctaggtg cagaaacaac atcaaaagtt actaataaag gtacaacaac
    47641 acttaaagca aaagacttat taggggaaaa tcctcaagtt gtagcaagtg caaaagcaac
    47701 agctaaaatt gataaaaatg ggcaagaaga ttcgtttgta tcagttaatg aaaatatgta
    47761 ctatgttgat ggatggtttt ataatgatta tgataataaa gagcgtggta atatttcact
    47821 agcgccaaag agcgggaaag gcaaaataaa aataaatgtt ggtaacattg attttgctaa
    47881 taaaaatgtc cttgctgtgt ttagtgaact atcaggcaca gcggttaatt ttggtccagt
    47941 tgattatagt caaattttac ttttagcaag tattactgga aatgttaagg ctcgtgaatt
    48001 tgctggtgtt ttaaccatta catataattt atccaaagat gatcttgtgc gatttgttgc
    48061 tgcacagatg gcgggtattg attatggtga tttaacgagt gaagaacgtg aattagtaga
    48121 tgccataatt agcgagataa ttgctcaact tgaaaaaggt tatgaagttc gcaagtacgt
    48181 aatcgaagtt aaaattgcta aagaaggtta tattactaaa gtacgtgttg aaaatgatgt
    48241 aacacaaact ataactgatc cgcaaggtat gggtgaagat cctgaatatg tcatagtagt
    48301 gaaatcaaaa ggatttaccg agattaatac aaaacttaat gaaaaagtag caattaagtt
    48361 tccagatttt agtgattatg ttgaacggtc attgatgtaa ataaataatg tcattagaag
    48421 agtcgccgct gtgcgacttt tttattaatc aaaattgtta tttttatatg tttctttaca
    48481 ataaaaagaa aaatacaatg ctataatgta tgaatagtag aaaggaggat acttttacta
    48541 tgaaactaag aaatttatta tggctcccaa ttgcggcaat gttactaaac agttgtgcac
    48601 ttggtaagaa agtcgaacca agcgttgcgc aagaacgcgc ccttgccgcg actaaagcaa
    48661 atgaagaagc agaaattgat acgcaagcga ttacgagcaa aagcgaaatt tcagacacaa
    48721 ttaaaagtta tgaaggtgaa actgttgtaa ctgaaacaat tacttcaaaa tttgaatcgt
    48781 catttaaagc aaaaggctta catggtgata caccagaatt tgccgcaact tacaaagaaa
    48841 tgatggaaga aaaagttgat ggcgatgaaa catattatgt aaaccatgaa gaagcatttt
    48901 atttaaagga tagttggctc tttggcggtt tctctacgac tgttcgtgaa agaggcgaaa
    48961 ccgaaactga agaaaacaaa ggaaaagtta aaatagttcc aattgacttt aaagttgttt
    49021 ccgtgtttga ttacttatct ggcttctcgg caggtgaata tcaatttggt gctcctaatg
    49081 aagaaggact tgctatactc tttcaggttg cttcagaaat tgaagccaaa gaactttttg
    49141 gcaaacttac aattacctat aagttagatg ttaaatcact cgtgcttatt atgttattag
    49201 cctttggtct agatttagat gcaatgactg aagaagaacg tgaagcattt gacactgaac
    49261 ttaatacaat ggttgaagaa atgagtaaag ttatgtcaat taataaatac caaatgaccc
    49321 ttggtatttc taaagatggt tatgttgaaa aaatacggac tgatattgat atggttacta
    49381 acaaatataa cgaagaagtc gaaccaaaag tattagttgc tagcgaagaa ttaaagacat
    49441 ttactgaatc aactatggaa attaataaaa agcttacaat taagtatcca aatattgatg
    49501 attatccaga agcgcaacca ataataagat aaaacaatta ttgctattaa tgacaagagt
    49561 cgctgcggcg actctttttt aaattgccac actgatgaaa aaatagtgtg ttgtaaagat
    49621 aaaaaaagtg aatctcctaa tgccagttat tacgatttta cacattatat tgtgtaattt
    49681 ataattagtg aaataaccgt gttttgccgc tgttttacca ttaaacaaca aaaaagtgta
    49741 tatacttacg cgtatttgaa tgttaaaatg aaggtgtagc aataggtaag taggtacttt
    49801 gctatgaaaa gaaaaagaat gttatggcta ccacttttag ccctacttct tggcggatgt
    49861 tcggtcgatt ttgattttgg accaacatca aaagattcag aaggaggagg cggaggcaca
    49921 acctcacaaa caggtggagc cgtcagtaga gacgaagcta atagccgtgt taccgcggca
    49981 tttactaatt cagatatcgc tgatgctaca acatatcagc aaacaacatt acaagaagta
    50041 actgatgata ctaaagaata tgttaacgac gctcttaagt atcatgtagt tgaaaatacg
    50101 aactctaatg tggtagttaa agcaacagga ctacaaactg atgatccagt agcgaaggta
    50161 acgggtgaac cacttgaaga catgactgaa gacggtgttg aaacatataa gcttattgat
    50221 aatcgtgagt tttattacgc tagtgaatgg atttattatc ataatgctac gcgtaagcgt
    50281 tatggtaacc cagaagaacc gtttattgac actgaagtaa aactaaaaca acatatgggt
    50341 ccaacaaatg gccaacaact agcggaagga cttgaagggt ttgatttagg tgacgcacca
    50401 gtgccgacaa tgtttattgg ctcagaatta gatgttttac aacaaacagc aaatattact
    50461 gcgacgaaaa atgggagaaa tatcactgtc atctatagca taaacaaaga tgcgtttatt
    50521 gcgatgatgg aaagcggctt acgcggaatt ctctttagtg aagaaatgac aagtgaagaa
    50581 cgggcagaag ccgaagaatt tctgcagtgg tatgttgcgc tcctagaaga agtgctcgat
    50641 ctcagagagt acaagattac aattggcgca gatggtaatg atcacttaac gcaagtgcgc
    50701 gcggactttg aattaattac aacaatggtt tatgaaccaa gtgaaacata tccgaaagga
    50761 tatacacaag ttggcaaatt gagtggtttc cttgatatga aaccaaaatt caatgaagag
    50821 ttcactatca cattaccaga ctttagtgat tatgaagaag gcataatatt tgaataaatt
    50881 ctaaaataag ctaaatggag ggagccgcaa atgcggctct ttcttttgat tttataatta
    50941 taaaaatatt tagtgaaaca cgattcactt agattagtct ttaagaaaat aaatatattt
    51001 tgatacaata gttttaatta cgaataatta aaggtgttat gtcatggaaa aatgggattt
    51061 atatacaaag gatgaaaaga aaacagggaa aattgttgat aaaggcgcat taatacctga
    51121 aggctattat catattattg ttgaaacatt aattaagcac acagatggta catatttagt
    51181 gatgcaacgc tcctatgata aaaaagaata tcccgggaaa tatgaaggaa gctgtggcgg
    51241 ttcaattaag gctggtgaaa caaaagaaga aggagcaatc cgtgaagtat ttgaagaatc
    51301 aggattagtc attaacgaag taacaccaac ttattacttt atccaaaaag atgtaccagc
    51361 aattaatcaa gggtatttag cgattataag cgcgaaagat aaaacaatta aatatcaaaa
    51421 agaagaaaca attaatcata aatggttaac actaaaagaa ttaaaagcat ttattaaaag
    51481 tgatgaatat aatgaaagtc acgcaagtag gttagtaaca tacttagatt cactaaatga
    51541 ataaatggta gaataatgat taataactaa aaggaagaaa ttattatgaa ggaaactaat
    51601 aagaaaaccc gtctcaaagg gtttatgaaa acaacagtcc taagttttca gcatatgttt
    51661 gcgatgctcg gtgcgactgt gcttgtgcca atgctagcgg gtctatcaat ttctattacc
    51721 cttattaccg cagggattgg gacaatcatt ttctattttg tgacaaaaag gaaagtacca
    51781 gtatttcttg gttcaagttt cgcttacttg ccagcactga tgagtattat ggcggaaagt
    51841 ggcgaagtgg ggagtcttgc gtggcaacaa aatatgggca aactagtaat tgctattttc
    51901 tttactggtg taatgtacct catttttgcc ggagtaattc gcctaattgg tgttgaaaag
    51961 gtgcgaaaaa tgtttccgcc gcgtgtcgtc ggtcctgtga ttattgtgtt agggatgtta
    52021 cttgtacctt caatcttttg gaataatatc attgccccat atgcgcttta tggcgctgag
    52081 gcgtggaagg tgtggacgac tgcgcttatt acactcttaa gtattattgc cattagcgct
    52141 tttgctaagc ccaaatcatt ttttaaagca gcgccaattt tacttggctt tttgattggt
    52201 tatgtatacg gagcaatagt tgggttagtc gactttagca cactcaaagg tagtgatatt
    52261 gtcatatttc aaagtattgg ttcgcttcta ggcttttata aatatttaaa gtttgattgg
    52321 aacgctattc ttatgatggt tccaattgct tttgtaacac tgatggaaca tattggagac
    52381 ataagcgcta atagtacagt atgcggcaaa gacttttttg ttgatcctgg tattgatcgc
    52441 actttagcag gcgacggttt tgcgattatg gccgctagtg ctttaggcgg accaccgcaa
    52501 acaacatatg gtgaaaatac tgctatttta gtaattacgg aaaactatga accgcgtaat
    52561 gtgttttatg ctgcaatttt agcggttatc tttggtgtga ttagcatttt tggtaatgta
    52621 atcgctacta ttccaagtgc ggttattggt ggggcatcaa ttattctctt tggaatgatt
    52681 agtgcttcag gtttacggat ggtcattgaa cataaagctg atattggcca aacaaaagac
    52741 ttaatcgttg taagtgttac tttagcggtt gggttaggac taggtgcgct tagtgtcgca
    52801 agtgatatcc ttagcgcggt tggtgtaaca acttttgacc cgcgtttact taaagtaatg
    52861 attggaaatg ttgaattatc tcccttagca attgccacgc ttgtagcggt attacttaac
    52921 ctagttattc ctaatccgaa ggtagttaaa agtgaagaaa aaaaggcatc aacagaagat
    52981 accttaacaa atgaaataaa ttaatttaag cactttcgcg taaaatgagt tcataaggaa
    53041 cgtttacact taattttagt gaacgttctt ttttcatgcg ttcgtttagt agtttaagcg
    53101 ctaattcgcc cataaattca gtgtggattt taactgatga taatgaagga gaagtaaatt
    53161 gcagggtagg aatatcatta acaccaaata tagcaatatc gtctggtatt ttaaaaccgc
    53221 gttcctggat agcttttgaa acacctaaag caatcgagtc attgccgcag aatatcgctg
    53281 ttggtgtgtt gccttcatta ttgtcaatta gttcgtttgc gagttcataa ccactatgag
    53341 cgctatattc accaacctta acataaggtt tataatataa cttgttttga tgcattactt
    53401 caatatattc taactcacgc ggatcaggtg ttttgtttgg ttcagaagaa acgcgctcac
    53461 ggccaccaat ataagcaatt ttcttatgac ctttattgat tagtaaatga agaacatcgt
    53521 gtgttagtgc gcggaaatca acgcgaacac aatcgcattt accatcctcg tcttccgaat
    53581 tagcgtacac aacagccgaa aagcgatcag aaataaatgt ttgtgtctct ctcgtaaaac
    53641 taccaatcat aatcacacca tcgtatttct gatcttcaaa aggatctgtg atgcgatctt
    53701 cacgataaat tacatcaaaa gcaatgccaa gttcacgaca tgctcgttgg acgccaattc
    53761 taattgaaat aaaataagga tcattaattt cttgttcgat tgtataccag tgataaatag
    53821 caatacggaa tttatttttt tgttgatttt tctttggttg caaatgttta gtgtaggcta
    53881 agtcttcggc aacttcaaaa attcttcttt ttgttttagc ttgaacattg atagttgggt
    53941 cgccgtttaa gacacgagaa acagttgcgg gtgaaacatt agcttttcta gcaatatcac
    54001 gaatggttgg cataagcacc tcatttcata ttaattagtt tcattttatt ataaggtctg
    54061 gtaaatatca acaattaaat taaaatattt actaaacaaa cagtaaacaa ttttaaactg
    54121 cgcaacaaat aacattgatt ttatatgaac tatgttaaaa aacaaagaaa ttacaacgca
    54181 aattttcgtt ttagccagaa ataaattacg cttgacattt agtaaacatg tgataatctg
    54241 taggtaagat aattaccaca tatttcataa cgattagatg aacctaagga ggaatctatg
    54301 ttttgttacc ataaaactag cgctagtaat aatgagaagt tttttcgtaa aggtattttc
    54361 ctatcgataa ctcttttaat agcgacaaca gttttaacat cgtgtaaggg aggtacaagc
    54421 gtgaatagcg aaatacggag tagtgaattt aataataatg aagtagttta tccgtctaat
    54481 cctgcctttg gtaataatgt tacggcacat gatccttcaa tctttaagga tgatgatggg
    54541 acctattata cttttggttc tcattttgct gttactaaat caataaactt aatgcaatgg
    54601 acccaagttg ctaatgatgg tggcgctaat gttttatatg ctcgtaactg gcgcactgta
    54661 ttagcggacg cttttgctca tgttggtgaa agacccggta gtacttgggc accttgtgtt
    54721 cataagtttg gtgaaaaata ttatatgtac tattctttat caacttttgg tagtagtgtt
    54781 tcttatattg gtcgtgttga aggagatagt gtgcttggtc catacgacaa ttctgtggaa
    54841 attgtaaaat ctgatggtcg cggtggacca aatgcaattg atcctgaatt attctttagt
    54901 aaagacggaa gattatggtt agtttatggt tcattttttg cgggtattta tattaaagaa
    54961 ttatatgcaa gcggcgaaaa tattgggtta ccaaaagaag atggctacgg caagctttta
    55021 tggcaaggtg gtgatcaagg cccagaaggt cctttcattt tctataatcc taacactgat
    55081 tatttctatt taatggcatc gcatggttcc ttatctacta attataatat gcgtgttgcg
    55141 cgcagtaaga acccagacgg tccttatact gacccgcgcg gtatgagtgc tgaaaattat
    55201 cgtaattcag gtctaaaact cgctggtaat tatcaatttc aaggttatcc aataggttat
    55261 gctgcactcg gtcataattc gattttaaga gaagacggaa aatattatgt tgtttatcat
    55321 tcgcgttatc gtagtggcgc atcaggtgtg acgggttatc ataatcaatt tgtcaatcaa
    55381 atgtttttta acaaaaatgg ttggccggta cttgcaccaa atcgctatgc gggcgaagaa
    55441 cttcaagtta ttacaatggc acaagcgtca aaagattatg atgtattaat acatacaggg
    55501 ggtgatagtg aagggtttgc tacatcagta acttataagt ttagccaaaa cggtgacttg
    55561 ctagatgctg aggaagatgc tatcggtaca tggagcatta acgaagatta ttatgtcacg
    55621 attaaaattg gcttaaatac atatgaaggt gtcattattc cgcaatggaa taacgatctt
    55681 caacgagccg gactttcaat tacggcttat agccgcaatg gcctaagttt atgggccaac
    55741 gaacatttta gataattata aagccaaaaa aggctaataa aaaaggagaa taatatgaaa
    55801 aaaagaagac tacttattcc acttattggt ttagcacttc tgcttacagg atgtaagaca
    55861 acatcaagtg aagaaaaacc agtaagttcg gaacagccac cagtaacaag cgttactaca
    55921 gaacaaccat cggtttcaag tgaagaacca ccagcaagtt cggaagaaac gccggttgct
    55981 aacattaaat ttggtgctga cccgttcaac ggaacttccg caacaggtgt cgcgggcgtt
    56041 ggtgttgcgc tttattacga cgcaactgaa gacgatgtcg ctgtcgcttt tgacaaagtg
    56101 gcatttactg ttaataaacc atcaggtgtt ctctttgatc cagtaattgg tttagtggaa
    56161 aaacttagtg tttattattt acgaccaatg gaaccgggaa catatacggt aacactgaca
    56221 gttgaagata gcgcaggatt aaaagcaact gctactaaag aaattgttgt ttcaaccggt
    56281 gatttatcag gtgatgttga tgcaattaaa gcggctattg ctgcgattta tccagataac
    56341 atttatacaa accttgacta tcgcactagt gaaggtgtct tcaatgaatg ggtcgttgtt
    56401 ggcaaaaact ttaccgtatt tgatcgcgaa gattatactg gtaactatgc aacagcattt
    56461 attccatttg cccgctttga aggtggtgaa gcattacaag atttcaccat ttctttcaaa
    56521 tacaccacac ttaatgaggt ttggaaatta ttatttagtt tctggaccgg tgaagtaggt
    56581 gctgataatt ttgcaggtga ctatctccgt ctattaacaa atcgtaatgc aattggtatt
    56641 caaggtgatg ctcaacaagc ttcagaattc caagatgaag gtgaagcaac tggtattcca
    56701 ttaaaagaag gaccagtttg gattaaattc acccgtcaag ttactaaagt cggtgaaggt
    56761 gattacactg ttgttttcaa actctttact tcaaccgatg gtgaaacatt tgttaataat
    56821 actaccgcaa cattagttaa ccaagttaat accgctggtg gcactgctgg tctattaact
    56881 ggattccttc cattctcaat cgacaatgat tttatcgttg aagatctagc tgtgagtgga
    56941 acagtattta cattagaata attttaaata aaagtgaggt tcttccagtg atcccctcgc
    57001 tggaagaact ttcagtaaag gagttttaaa tgaaaaaact acgttattta cttatattgc
    57061 tctctgctag tcttgtatta agtggttgta aaggtggtaa agtttctgaa gaagaaacat
    57121 tacccgaatt aacaaatatg acagtagatg gcgtaataaa tgaagaagct tataatgtca
    57181 ttcctgggcg cacagcgggt gttaaagcag gagtattagt gcagtatgca gttggtagag
    57241 agggtgtcta ttttggctta actgtgacag acacaaaaca tcaagtttct gcaccaagtg
    57301 ggattgttgc tagtgactat gttggtcttg cggttgatgc ccttgctgtc ccaaagagtg
    57361 atgatggtgt gtctgaatca acaaagttgt tccgcgtcga tacgatgggc cgttatactt
    57421 acactacagg taatgaatat ggtgcatggg ttgatgttga tagcggagtt tatgaaggcg
    57481 ttataagtgg agaagaaatt ccgacaattg ctgttaatat cgttaaagat cagtattatg
    57541 ttgtggaaat attctttacc tgggaacatt taggcacaac tgaagaagca gcaaaagacg
    57601 ctaattgttt aatgtattat attgaacacc gtgatatggg tgttgatatt cacgctgatg
    57661 caaacattct tgcgccggca atgtacaatc gccttgttta tcttggtaat cgtaaaggta
    57721 gtaaccttcc agcagatcct ccagaaataa caattgatgg cgtcctcgat gaaacattat
    57781 gggacacagc ttttgtcact aactctggca attttaagga acgcgttcct gatgaaaccg
    57841 ctggtgatta tcgcacatta gcaatttggg gtgaaaaagg tatttatctt ggtgttgaag
    57901 ttgaagatcc acatattgaa gcaccaaatg gtactggagc cgcttataaa aatgctggta
    57961 tggaaatgcg tatacatgtt tataataagg acggtttacc acttaattca cttaaatggc
    58021 tctttgatgt atggggacca caatggcacg aaactggcgc tgggggtcta gcatcatctt
    58081 ttgcgccata tgctgaatat aaatttgtga ttagcggcac aattgataat aacgaagata
    58141 ctgatgaagg atggagtttt gaaatttaca ttcccttctc tgaactcggt gttactaatg
    58201 ttgactccga ttatataaaa attcttcacg ctgttggcag tcatgaacaa aataatatgc
    58261 ttccacaaga atacttagac ttgcatccag atgtaaactg ggactaccct gaagattatc
    58321 cacgcatttc aaaacccgtt taaaggaggg aataatatgt ttaaaaagaa atggatgcca
    58381 ttattactat cgattccact gcttttaact ggttgtaaag gtaaaacaag tgatagtgtt
    58441 actatcttta tttatggcca agctcatgag cgaagtattt attcaaagat tattgaaaag
    58501 tttactgaag agacgggaat taaagtcaat gctgaactcg ttaacacgga tgggtatgct
    58561 gagacattaa ctgcatcttt aggaacaaaa aacgcgcctg atgtctttta ttcaaaccca
    58621 gcgtcaattg gtcgttatgc gatgaatggc gttattctcg atcttaaacc ttatttagaa
    58681 atagaagcag aagtggagga tgggcttgat tataaaaatt tcttagatgg agtcgttgat
    58741 tactatcgtt atgatgtgac aaccaaaaag cgtggggaag gggaagccgt atgggcaatc
    58801 ccaaaagatg tttcctctta tcctttaggc tacaatcgta atattattaa tctgtcggtt
    58861 gagcttggtc actgggcaac acatacgaaa aaaggagcat gggcaagtcg tgaaggcggg
    58921 atgcctttgc cgtgggaaaa tgatccgcgt actgacaaac aaatcgtata tacatttgaa
    58981 gaatttacaa atgcatgtcg cgctacaaag tttattcata acgagattga atattttggt
    59041 actgcgctaa ttgatacatg ggcaatgcat tcatggattt gggcagcagg tggtaactgg
    59101 ttatcagatg atggtaagac ggttactgtc aatactccag aatttatcgc tggttttact
    59161 gaattcattg atttaatgga taaccgcggt atttctcaaa cacgcgaaca aatgggggtt
    59221 aaaccacact atgatcgctg gatggcgggt gaagtagcct tctttaatgt tggtgtatgg
    59281 gatgttgccg catttgaaag tgtaccaaaa gaattattag attatggttt aatgccaaca
    59341 ccacgaaaag ataatgctag tccagagtgg tatacatata ttggtacatt aggttacgct
    59401 gtttcttcac gaacaagagt tgcgcaagaa gcagttaaac tagcgcttta catgagtatg
    59461 agcaccgaat cgtataatcg tatgactaaa tatcaaacaa ttcaattacc aaataacaag
    59521 ctaatggttg atgagtatct tagtgatgaa acaattaaac ctgaaaacaa aggaattttc
    59581 cttgatgtta ttcaaaaagg acacgggaaa ctccatccaa cagccatggc ttataactca
    59641 ttatggtatg acgcttttat cgctgaatta tcacgcgttt ggaaagttac tgatgatgac
    59701 ccaagtggtg aaatgtcgcc agcacagtac gctgcttatg tgcagccgat aatgcaggct
    59761 caacttaata ttgcactagc cgatgaagcg gctggtgtgt aatttattta tatcattgct
    59821 cggacatcga tcctctcgat gttcgggcat agattagatg agtaatatat tatgaacaca
    59881 aatattgtag aaaaaccaaa aaaaagaaaa gttaaacatg ttcaagaaaa cataatagga
    59941 tatttattta ttttgccagt tttcattggg attgctttct ttatcgtata tccttttttc
    60001 tattcattgg tttcaacttt taaaaactgg aatggacttg ttccccttag tgttgcgccg
    60061 acagttggat ttgagatgta tcggcgagtg ttaagtgatt cattattttg gcaagccttt
    60121 ggtaataccc ttattaccct tattgggatt ccaattggga tgattctttc aattgcaatt
    60181 gcaatgttgt taaaccgcgg acttaaattt accggcattt tccgtgtaat tttctatatt
    60241 ccagttgttt cctcaattac tgcaattatt atattatggc gagcaatgat gaatacccaa
    60301 ggaccaatta atgatttttt agctctcttt ggtgtaaaag caataaactt ccttggtgaa
    60361 ccaggatggg cccgtttctc gcttattatg atgcttgtat ggaagggtct aggaacatca
    60421 atcttacttt atttagcggg tttacaaggc atttctgata cttataaaga agccgctaaa
    60481 attgatggtg ctaatgactg gcaaatattt tggcatgtta ctttcccttt attaggcccg
    60541 acgcatttct ttattgtagt gaccggtatt atagggggaa tgcagcttta tgttgagcca
    60601 gaattacttt taggtggcgg gccaagtaga gcaaccacga ctctcttaat ttacttattt
    60661 gagcgttttg gtgcccaccg tattagcgaa gcttcagttg tcgcgtgggt gctaggtgtg
    60721 tttgtcttta ttattactgc tattcagttt tatacgaatt atcggaggga aaaagcatga
    60781 aaaagtcgaa tatcaaagca aatcgttcaa ttaagcgtgc caaactcttt tccgatatta
    60841 gcacttatgt aattttggta cttggatcag tgttaatgtt agcgccactt atttggatgc
    60901 tatcagcgtc attaaaagtt aaccaatttt ctgtttatgg tcctttattt catgaatgga
    60961 acttctctaa ctatatcgaa gtgtttaaaa caattccagt gtggaagggt cttttaaata
    61021 gtgttatagt cgccgttccg tcaattatag taggtacatt tgtctctggg ttagcggctt
    61081 atgctttcgc aaaaattaaa tttaaattct gtaatataat ctttatttta atgcttgcgg
    61141 ttatgatgat tccttttcct gttattatga ttccgcagta ttacatattc tcagatttag
    61201 attggattgg aactttatta ccacttatta ttccaaagtt gttcggtaat attatgatga
    61261 ttttcttctt acggcaattc cttacacata ttcccgatgc tttggttgag agtgctaaaa
    61321 ttgatggcgc taatcatttt caaattttct ggataattat tttaccgctt ttaaaacccg
    61381 ctttggcggc gcaaacagtg ttatggttta ttggcatttg gaatgattac ttagccccca
    61441 atttcttctc agcggcttca ccaactttac ccgttgttat tggtggatta gtaagtgact
    61501 tcccaatcca agaaccagtt catttaaata tggcagcgtc aattatttca atgattccag
    61561 ttctcgcaat ttttgctatt ttccaaaaac aaattattaa ttcattcgtt ctctcgggaa
    61621 tgaaagaata agaaggagta acaatatgaa aacaaaatta tttatcgaca aaaacctaac
    61681 gattggtaaa gttagtaatc ttctttttgg gagttttatt gaacatttag gaagagcagt
    61741 gtatacgggt atttatgaac caggtcaccc taaagctgat aaagatggct ttcgtcaaga
    61801 tgttatcgat ttaattaaag agcttaaagt cccaatggtg cgttatcctg gtggtaactt
    61861 tctttctgga tataattggg aagacgggat tggtccgaaa gaaaatcgtc cggttcgcct
    61921 agattacgct tggcaaacta ttgaagataa cacattcggc actgatgaat ttatgcgctg
    61981 gtgtaaaaag gcggaaattg aaccaatgat ggcagtcaat ttaggaacag gcactccaaa
    62041 ggacgctggt aacattgttg aatattgtaa ttttccaaaa ggaacatatt acagtgattt
    62101 acgaattaaa aatggacata aagaacctta taatattaaa tattggtgtc tcggcaatga
    62161 aatggatggg ccgtggcaaa tatgtcacct aaatgctgaa gattatgcta aaaaagcatt
    62221 agaaacagca aagattatgc gctgggttga tccttctatt aaattaattg cctgtggcag
    62281 ttcaaatgct gaaattgaaa catatcctga ttgggaccgt attgtgatgg aaacacttta
    62341 tgatcatgtt gattatattt ccatgcatcg ttattactgg tacgaagata gtttagaaga
    62401 tttctttgct tcatatcatg atatggataa tttcattcat acaattaaaa gcactgctga
    62461 ttttgtgaaa gctaaaaaac gttctgataa agtaatgaag atttcttttg atgaatggaa
    62521 tgtatggtat ttaaataaat ggaaaccagg tggttggaaa aaagcaccgc atattcttga
    62581 agaccattat tcacttaaag atgcattagt cttttcagga ttactaaata cgctagtaaa
    62641 taatgttgac cgcgttgaaa ttgctgcttt agcgcaactt gtaaatgtta ttgcccccat
    62701 tttcacaaaa gaaggtggag gggtgtttaa acaaacaatt ttctatccgt tttatgctgt
    62761 gtcaaattat ggtcgtggtg tcgctttaaa agcaattaaa acgggaccta aatttaattc
    62821 aaagttcggt gaagtaaatt atgtttcaga agcagtaaca tttaacgaag agaaacgaga
    62881 aatttctgta ttccttgtaa attatgcgca agagaagatg gaagttgaac ttgaattacg
    62941 ttcgtttggt cgtgtagaag cacttactca taaagtaatg acgcatgacg acttagaaat
    63001 taaaaatacc tttgatgaag aaaatgtcaa atttgttgaa ggtgagttac caacagttaa
    63061 aaatgatgtt gcgaccttgg aattaggccc gctttcctat catgtctatc gttttaagta
    63121 ctagggaggt actagtttga agcgttatct tttcttaatt agtgtggcga tccttgcttt
    63181 aaccgcttgt agcgggggta aaacaattac ctctatttct aatagtgaca atattgaaga
    63241 agcaattatt tatcctacta agccatatag tgatagtgat cacattatgc aaaaagatcc
    63301 ttacgccttc tataacattg cgggtaacga cactcgctac tggggagcgg ctaacacgca
    63361 tgatccagca attattaaag aaggtgatac atattacgct ttttcaacag acgctaattt
    63421 tggtgtaacc tcacaaaaag ggattcatat ccgcaaatca aatgatttaa ttaaatggag
    63481 ttttgtagga acggcgttag atgttacaac acttgaagat caaattgaat atattggtta
    63541 taacagagac ggggaaaagg ttgatttctt ttgggcgcca gacattatta aaagaggtaa
    63601 tgaattttgg ttattttatt caatcagtgg ttttggacaa cgcaattcaa tgatgggatt
    63661 agcaaagtct aacaatattg aaggaccata cacttttgat aaagagatat tacgtacaag
    63721 ccaaagtgta ggtggcacgc caaatgcgat tgattccgct atatttgtgg aagaagttga
    63781 tggcgaagaa aagatgtata tgagttatgg ctcatggtca gcaggcattt acattatcga
    63841 acttgatcct gtgacgggtt atccgcttat tgcccaaaca cttgtcgaac gcgaagtaac
    63901 agttaacact gctgaaaaag gtgtaacagc aaccgctact aaattagtgc cctcctccag
    63961 cgatgatcct gcttttggta cgaaaatatt aagtattaca tctagcgaag cgccttacat
    64021 tattaaagaa aatggttatt attatttatt tattacgaac ggtgttgatt taacttatga
    64081 ctatgatgtg cgtgtttttc gctcacgcaa tatcttaggc ccttatgttg atggtaaggg
    64141 tgttagtgca cttagtgcaa caaattataa ttcatttaga ccttatggca acaaagtgac
    64201 aattgcccat caatttacat atcttacaga tgatattagt tataaacgtg gctgggcagg
    64261 tattggtcac tcaacggcat ttaaagatga agagcagtgg ttctttgcat cgcattaccg
    64321 cggcacttat atggatcgtc atcgtttctt tcttggtatt tggaagatgc attttgttaa
    64381 tgattggccc gttatagaac ctaatcgtta tactggtgaa ggtagtatta atttagaaca
    64441 agctgatata agcggaaatt atcgcattca aatcctgcat caatccgcgg ttaattcaat
    64501 gcttactgat gacattattt cgattgttaa tcgcgctgaa gagattacat taactaagca
    64561 aaaagcaggt gaatggtatg ttgtaaacgg agaatataac ggtcgttatc gccttaataa
    64621 caatcaactt gaacttgaat taaatggggt gttatacaaa ggagttatta ccccgcaatt
    64681 taattatgaa tttgaaaaag gagtgctggg cttatcactg attaatagtg atggaacagc
    64741 attatggggc aaccgtatta tttagaaagg aagaaatgtg tatttaggaa ttgaatttgg
    64801 ctcaacaaga attaaaggga ttgtcttaaa tgataatttt gaagtcattg cttcaggatc
    64861 ttacatttgg caatcaacac ttaaagatgg gtactggact tatgatctta atgaagtttg
    64921 ggtcgggatg aatgcagtaa ttgacgaaat tgctaagacg attaatgtaa aagaaatcaa
    64981 atcacttggt gtgtcagcaa tgatgcatgg ttttttacca tttgataaaa acggaaaatt
    65041 attaacacca ttccgcacat ggaaaaacac gaacacaaca gtagcggcca cggagttatc
    65101 aaagcaattt gcatttaata ttccgttacg atggtcaatt gcccatcttt atcaagctta
    65161 tttagataaa gaaagttatg taaaagatat tgatactttt actacattag caggttatgt
    65221 ccatctaacg cttaccggta aaaatgtatt agggattggc gacgcaagtg gggtgttccc
    65281 cattgatgtt aaaactaagc actataatca aaaaatgatt caaactttta acgatttaac
    65341 agatatgaaa cttgaaaagc ttttgcctgg tattgttgaa gtaggagaag acgctggctt
    65401 tttaacagca aaaggtgcgg agctcttagg gaataaatta acagtcggta tccctttagc
    65461 gccaccagag ggggatgcgg aaacggggat ggtatcaaca aacgccctta aacctaaaac
    65521 gggtaatgta tccgcgggta cttctgtttt ttcaatgatt gttttagaaa aggcgttgaa
    65581 taaatggtat cccgaaattg atattgtcca aactccaaca ggcgatgcag tcgcaatggt
    65641 ccatgcgaat gaatgtactt cacgtattga tccatggatt gaacttttcg gtgaagcaat
    65701 tatgagagtt aatggtgaag ttgatcgtaa tcatcttttt gaaacacttt atgaagtttc
    65761 tttagaagat accccgcttg gtgaatttat gaagcagaaa cttgttgaag caataattga
    65821 cttaaaaccg ggcatggata tcttactaaa caaagagcat gttaaagttg attatttatt
    65881 agggcatggc ggatacttta aatcaaagag cgcgggtagg aaagtgatga gtgaaacact
    65941 tggtattcca attcgtttat tagagaccgc tggtgaaggg ggcccgtggg ggatggctgt
    66001 cttagcagca tatcgttatt acaataaaga gcaaaaaata aaatttgata aattcataga
    66061 aaaagtattt aaaggagcgt aatttatgaa aacattttgg tttatcgttg ggtcacaatc
    66121 tttatatgga gaagaaaccc tcaagcaagt agaaaaaaat gcaaagaaaa ttgcaaaagg
    66181 actgaaaacg aaatatccta tcgtctataa ggagacagtt aagacacatg cagaagcaat
    66241 gcgcttaatt aaagacgcaa attatgatga aaattgcctt ggtatcatta ctttttgtca
    66301 cacattctcg ccatcaaaaa tgtgggtgaa tgccttacaa aatttacaaa aaccatggtg
    66361 tcatttccac acacagtttt atgatgaaat tcctaatgac gcgattgata tggattacat
    66421 gaatcttcat caatcagcgc acggagatag agaacacggt tttatcggta ctcgtttaca
    66481 aaagaatcgt aaaataattg tcggttactg gcaagatcct aaaacaagcg aagcaatttc
    66541 tgcttggcaa gatgttgttg atgcagttaa tgaaagtaag acattaagtg tcgttcgctt
    66601 tggtgataat atgcgtgaag tagcggttac agaaggtgat aaagttgaag cgcaaatgaa
    66661 atttggttgg caagtaaata cgattggtgt tggtacatta gtcgatacaa tcaaagccgt
    66721 tactaacgaa gaacttacag caaaactagc ggaatataaa gcaaagtatg cactgaacac
    66781 tgataacttt gcatcaattg aatatcaagc taaagcagaa gttgcgatta aaaagattct
    66841 tgacgctaat aacgctaaag cattcactaa tacattcgaa gatctctata acatggaaca
    66901 actcccagga ctagcaaccc aaaacttaat gttagaaggt tatgggtatg gcgctgaagg
    66961 ggattggaaa acagcagcga tgacacgtat ctttaaagca attggtaaag ttactacttt
    67021 tatggaagat tatacttatg actggaaaga agggctaata ttaggcgcac acatgcttga
    67081 agtatgtccg tcaattgctc gtgaaagacc gaaaattgaa gtccatcact taggtattgg
    67141 tggcaaaaaa gcaccggcgc gacttgtctt tgaaggtaag cctggtaaag ctaaagcaat
    67201 ctcactaatt gatttaggtc atcgtttccg gcttattgct cttgacgttg aatgtgttgc
    67261 cccaactaaa tcaatgccaa acttacctgt agcgcgcctt atgtggcgac cagaaccaaa
    67321 tttagaaaag tcagcagctt gttggttagt agcgggcggg gcacatcaca ccacactagt
    67381 gtatgaccta tcacttgaat ttctccgtga ctttgctaat ctccttgaca ttgaatttgt
    67441 gcatattggg aaagacgctg atccttacat acttgaagaa atgatgaaaa ataacgatat
    67501 ccgctatcgt ttacgagggt aaaatatgta caaaacttta aaagaaacga catataacgc
    67561 taacattgcc attaagaaat cgggtttagt acttttaaca tggggcaatg tgtctgttaa
    67621 agatgatgta cttaaagtaa tagcaattaa accgtctggt gttgcgtatg aaaagatgaa
    67681 agcagatgat attgttgtag tcgactttga cgggaatatc attgaaggaa ctttacgacc
    67741 gtctgttgat ttgccttcac acctagcctt atataaagca catcaagaaa ttggtgcaat
    67801 tgtccatact cactcaacat ttgcgacagc gtgggcacaa aaaggtagag caataccaat
    67861 gtatggtacg acacacgctg attattttag cgatgatatt ccgtgcgctc cttatttaac
    67921 accagaagag atgaaagact acgaaaaaaa tactggtgaa gtaattgcgg cgctcattaa
    67981 aaaaggacgg gctaatgaaa caccagcggc aatcatcaaa ggacatggtg cttttgcgtg
    68041 gggtaaaaat gctaaagatg ctgtgctgca cgctatcgtg ttagaagaag tagcgaagat
    68101 tgcctatctt acagaagcaa tcccgggtca aaataatccg ctaccagagc atattaagaa
    68161 aacccattat gagcgaaaac atggtcctaa cgctcgttat ggtcaaaaga aataagcact
    68221 aaacttttaa ataattataa aaaggcaccc tttgtaaaaa atgaacagcc ccttgtcaag
    68281 tagacacggt aaataatata aaattagatg gccatatacc tatactctaa aggtgataaa
    68341 ctaccgcctg gtcattttca tattattgtc gaaacacttg ttaaacatag cgacggcact
    68401 tatttagtaa tgcaacgtga ttatagtaaa aaagaatacc caggtttata tgaaggaagc
    68461 gcaggtggtt ctttaataag tggagaaacc aaaaaagaag gggccatccg cgaagtaaga
    68521 gaagaaacgg gacttataat aaatgatgta agtccaacat attattttat gagtgaaaaa
    68581 gacggcgtta taagtcaagg ctatattgcg ataataagtg gtactaaaga tattacttat
    68641 ttaaaagggg aaacaattaa tcataaatgg ttaaccttaa aagagttaaa agcatttatt
    68701 ttaaccgctg aatataataa agcgcacgct tcccgcctta aaacatattt agatacatta
    68761 gaagattaat aataaactta gcaattattt gctatagaag aggtatatat gacaaataga
    68821 aaagaacgga ccaaaggatt ctttaaaaca acaatcttaa gtttccaaca tatgtttgct
    68881 atgcttggcg caacagtgtt agtgccgatg cttgtcggtc ttgatgtttc aatcacactt
    68941 attaccgcgg gtattggtac aatcatcttt tactttatga caaaaaagaa agtaccggta
    69001 ttcttaggga gtagctttgc ctatcttcca gcgatgatgg caataatgag tggcagtggc
    69061 ccgattggtt caacgttgtg gcaacaaaaa atgggtgctc tagtggtcgc tattttcttt
    69121 accaggttag tttacctcgt ttttgcgggg gtaattaccc ttattggtgt ggaaagagta
    69181 cgcaaaatat ttcccccacg cgttgtcgga ccaattatta tcgtcttagg aatgctttta
    69241 gtgccgaatt ttatggaata acattattgc cccgtacgcg ctttattccg gggaagcgtg
    69301 gaaatcatgg acgactgcgc ttattacttt aataacaatt attggcatta gtgctttcgc
    69361 caaaccaaag tcctttttta aagctgcccc aatcttgctt ggtttttttg taggttacat
    69421 ttacgccgcg attattggct tagtggactt ttcttcatta caaaacgctg atataatcgt
    69481 cttccaatca attggggata aattagggtt ttatcgttat ttacgttttg attggagtgc
    69541 gattattatg atggtgccac tcgcctttgt aactttaatg gaacatattg gcgatataag
    69601 tgcaaatagt actgtctgtg gtaaagactt ctttgtcgat cctggcatta accgtacctt
    69661 agcaggcgac ggcgttgcca ttatggccgc aagcttatta ggtggtccgc cgcaaacgac
    69721 atacggcgaa aacacggcta ttttagtaat taccgaaaac tatgaaccga aaaatatatt
    69781 ttatgcggcc gtgttagcta tcttctttgg ggtcttctcg atatttggtt cagttataag
    69841 cactattccg agtgaagtaa ttggtggcgc ctcaattatc ctatttggga tgatcagtgc
    69901 ttcgggctta cggatggtaa ttgaacataa agcggatatt ggacaaacga aggatttact
    69961 tgttgtttcg attactttag cggttggtct tggccttggc gctctaagtg tcgcaagtga
    70021 catcttagcc caagttggta cagattttga cgccacgcta ttaaagataa tgattggaaa
    70081 tattgaactt tctccgctag cggtcgctac gtttgttgct gtatttctta acttagtaat
    70141 tcctgaacca aaagtaaaag cagaaactcc tattttagaa aatgaagtaa aag
//
LOCUS       DAOSTA010000002        64991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_476_length_64991_cov_11.186661, whole genome shotgun sequence.
ACCESSION   DAOSTA010000002 DAOSTA010000000
VERSION     DAOSTA010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 64991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64991
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_476_length_64991_cov_11.186661"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..354
                     /locus_tag="PLU08_00315"
     CDS             163..354
                     /locus_tag="PLU08_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ70187.1"
                     /translation="MINYAQKIREYRERKFLTQTEFAALLGVSIASVTRWETGHYQPT
                     MKVKKKIYNLLVEAGIEIE"
     gene            530..1384
                     /locus_tag="PLU08_00320"
                     /pseudo
     CDS             530..1384
                     /locus_tag="PLU08_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214884.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
     gene            1542..2051
                     /locus_tag="PLU08_00325"
     CDS             1542..2051
                     /locus_tag="PLU08_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014969041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOQ70188.1"
                     /translation="MCQKTFEEKLAELDACIEKHQKSPGPLMPTLHDAQEIFGAIPLE
                     IQKHISKKLGVSVAHINGVVTFYSRFSLTPKGKYIISVCMGTACYVKGAQAILDAVQS
                     ELKLKPGETSEDGLFTLEATRCIGACALAPVYSINDEIYGGGTSKKILEDLRKIIKAE
                     EEKNDQNKA"
     gene            2032..2397
                     /locus_tag="PLU08_00330"
     CDS             2032..2397
                     /locus_tag="PLU08_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70189.1"
                     /translation="MIKTKRDLEVIKKRMQPDFHLRKEEHKYRLLFRYNEKTVIQNIE
                     KIIDLCLAAIYQDKLDAVAIKVYDTRVEDKLILVVKEKNRAVRYNIKNEAMVTEILNK
                     HIGQGKVVYEYVDVIDKEA"
     gene            2400..4193
                     /gene="nuoF"
                     /locus_tag="PLU08_00335"
     CDS             2400..4193
                     /gene="nuoF"
                     /locus_tag="PLU08_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOQ70190.1"
                     /translation="MHKHYEIVLCGGSGCVSSKSQLIKQKFEEILARENLSDFVKVYM
                     TGCFGMCEKGPVVVVYPDQTFYAKVKITDVEEIVESHIKKGLIVDRICHHEFDEKLQK
                     MVYKNLHEMKFHSAQKRLALRNCGVIDPDEIDEYIGRDGYFALEKALRMEPKEIIQLI
                     TDSGLRGRGGGGFPTGLKWQLAERFRGGQSYVICNADEGDPGAFMDRSILEGDSHAVI
                     EAMIINGYAIGANQGFVYIRAEYPLAVKRLQNAINQAYKYGLLGKNIMNSGFDFDLEI
                     RLGAGAFVCGEETALISSIEGRRGMPRNKPPFPAEHGLWGKPTIINNVETYANIPQII
                     LNGPEWFQKIGTERSKGTKVFALGGKINITGLIEVPMGTTLREVIYDIGGGIPNGKKF
                     KAVQTGGPSGGCLTEEDLDTPIDYDTLIQRGSMMGSGGIIVMDEDNCMVDVAKFYLDF
                     IVDESCGKCTPCREGTRRLYELLEKITEGKGTLETLEELKQLCYDVKDTSLCGLGQTA
                     PNPILSTFNKFYDEYVEHVIDKKCRAGVCKSLTTLYITDKCIGCTKCVRNCPVNCITG
                     ELRKLHRIDTTKCIKCGACIQNCPVHAIIHV"
     gene            4208..5971
                     /locus_tag="PLU08_00340"
     CDS             4208..5971
                     /locus_tag="PLU08_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOQ70191.1"
                     /translation="MKKVNITIDNKKLEVDSNITVLQAATDADINIPRLCFLKGISET
                     SSCRICVVEDVGRNRLVNSCTYKVYEGLNILTTSPRVIKQIKINLELIAGNHQFDCWA
                     CSREHNCELLELLRRFNIDNHYNINKEIKKREWIVNKSESMVLDSSKCILCGRCIDAC
                     NHYTGLGILGFNNRGFMTSVGPAGGVNIGEAGCIYCGKCIQSCPTGALREKDDVDRAF
                     DLLDEEGAYVVAQIAPSVRATLGEEFGYPMGTNVEGKIYHALEKLGFHDVVDVNFAAD
                     LTIMEEGTELIGRLKAFLDGDKDVKLPLFTSCSPGWIRYIERYAPEYLGHVSSCKSPQ
                     GMSGAIIKNYYAQKLGIEREKIKVVSIMPCIAKKYEASRPEMEVDGVRDIDLVLTTRE
                     LARFIKRHVIDFDMLTNKQLTSPLASYTGAGVIFGVTGGVMEAALRTVKALLEKEDAY
                     LIDIREVRGFESIKEATLTIDGKELNFAVVHGAKHFKEVLELVKSGKKQYAFIEFMGC
                     HGGCIFGGGQPITKAKDFEKIDVLKERAKALYRIDQSGKIRRSHENPHVLKLYEEFLG
                     HPGSHLAHKLLHTTYSKKDVY"
     gene            5972..6865
                     /locus_tag="PLU08_00345"
     CDS             5972..6865
                     /locus_tag="PLU08_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70192.1"
                     /translation="MKKKIATLLLTSLLLGCQKQTVKLSLLVPAGSPLLTVAGVTSEL
                     EKEVTVGSDLIPAAFTKGEKDLIAAPIIIGAKLFNAGKSNYQLAAMLGWGNLHILSRT
                     LINTIDDLSGKSGLAFAEHSTPGIMFKLATSDVSDINVNYVKAVSDVGGPFIQKQYDY
                     VLISEPVLSKIRSNINDETYTFSLQNVAKLPKIAQFGLFSNPATKAKDVNKFLVKLKN
                     NVEQLNTDPESYVDSIIDSDDYFKGLGRDVLIDAIPRLDIEYKSALEAKADLTLFFDY
                     LISVDTNLIGATSLDESFFRS"
     gene            6843..7607
                     /locus_tag="PLU08_00350"
     CDS             6843..7607
                     /locus_tag="PLU08_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70193.1"
                     /translation="MKASSVPKKKPTAQIIASSLGFLTIILLWIIISALHQNDIIFPG
                     IISTLKVFFSLFQRGSTYFILLNTIGGLILVLVLSFLTALILSLISLKFPTFKSYITP
                     FLALFKIIPVPALLIVFLVNFKQSFIPFLLTYIVLLPLVYDGLYGAFISINKDLIDEL
                     KITSKLNLLMIFKIYIPIVSKQVISTLLQALGLGLKVKVMSEFIAEAPNTIGYELSYG
                     RATLSMDIVFAWSLILIVIVVILDAVLVNYLGKQEA"
     gene            7693..8715
                     /locus_tag="PLU08_00355"
     CDS             7693..8715
                     /locus_tag="PLU08_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOQ70194.1"
                     /translation="MEAWQIILIVIGAYFLVLFLYSLLIYIFTSVRSKMRCDKNPLFT
                     YYTYEDYQGLKAETFNFKNFKGDNLMARMYTRDGFKDDTYLVFFHGIGAGHEAYTTLI
                     NDLVTTLEMPLLTFDYTGCDFSEGKSIVSVYQPLVDAHAFLAHLQTMPQYKGKKLVLI
                     GHSWGGFVAANLYPFNKDKNIIKVVNLNGVTDFGLYIKHSAKAPFIFVPLFKLFNAFR
                     YGKFAFAATRNSIKNTQIPHLIVHGEKDSVVDFNLFIGNLVLQEDKHKNIKFHFESDK
                     NHSAYLTTDGEKALGEVMGYFLTYSKQKNKDETLKQKIVDFDFSRAVENDPKTLEVIR
                     RFVKGD"
     gene            8718..11123
                     /locus_tag="PLU08_00360"
     CDS             8718..11123
                     /locus_tag="PLU08_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOQ70195.1"
                     /translation="MVNEELKTLLHTLSLTTKLKLLTGANFWESEKVLGTDLPIIVMN
                     DGPFGVRKPTVNVDSGMLMEVIPATAFPTTSALASSFDPELLEKMGKTLATECIDQGV
                     DLLLGPGINIKRSPLCGRNFEYFSEDPYLTSEMASGLVRGLQSKGVGATLKHYAVNNQ
                     EKERYTTNAIVDKRALNEIYLRPFHNVIKNTEPFSVMCSYNQINGRHASRHYKLLTEK
                     LRNEANFKGLVVSDWGAVVDHVDSINAGLNLEMPGGYQNTAELEKAVKDGEIKLETID
                     EMITPLLKFMMYKRDNPKPKVKCDYKKNLEVAQEIAEKSAVLLRNEDNLLPINKTAKV
                     GLIGNFAIKPRYQGSGSSQINPHTLVSVHDAFIQNDVNFVYADGYAEHEVKPNKKKIA
                     EAVKVAKNSDVVVLCVGLPESYEVEGYDRKTLDMPPSHNELIKEVAKVNENIVIVLST
                     GSPVTMPWINDVKSILLTHLAGSMTGPATYNLLFGVTNPSGKLSETYPLSLDKGLPGG
                     EFLTSDQNVLYRESLYVGYRYYDTFREQVLFPFGHGLSYTQFNTSHHEIEEVKDGYNI
                     TMEVTNIGHIRGAEVIGIYTGKSRSLLYGPRKELRAFKKVYLDPGEKQKINIFLPTES
                     LKAYCPVKQEWVLENVNYNVYVGINVQTTDEIFQILVDDANDLSYLKNYQKENEMYYR
                     RADFNPSIKDFTTLFGGESLPLFFANRKRPFTLANNISDTTDYWWGRTIYKIMAKTSR
                     KLAGDDVVLQEMAVETIKELPFRSLSALSSGEVSTNQRDAILLLVNGKPIRGIFKFLR
                     KKK"
     gene            11195..12484
                     /locus_tag="PLU08_00365"
     CDS             11195..12484
                     /locus_tag="PLU08_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOQ70196.1"
                     /translation="MSEEKVSTTQDLKVQKQTKRDRRYLINIIVILLITFGYTTYSLW
                     NELPAVINVFRGTEADYRMIIIIIVLIALKFFIEGIILFIFARLYTPTYKIHRGVANA
                     FIGQFYNDITPSATGGQFAQVKTFAQQGVPVSIGASIMVMHFILYQIVLVVFGGLLIL
                     LNLGEFARLGSVSIFKLNIPVWVFASFGFLANLVTIVGLFLLAYSRRVHHFVINVVVD
                     LLAKIKIVKRPEYRKNKLYTSTENFRIELRRLSSNVPASILIMVLFFLKFIVNYSVTY
                     FVALMLNPALYGGLSFWETISKTGFLGMITGFIPIPGAAGFAEYFYEIIFSPVFGGDA
                     AFTKAVQLIWRVATFYVNLIVGGFVTAFYRSSMEEIVDETGHVKTFEEVKSYTFAERR
                     ETSVTMYATSQLSIKEIQRRLSPKKKRRWRNHKEDQE"
     gene            12484..13653
                     /locus_tag="PLU08_00370"
     CDS             12484..13653
                     /locus_tag="PLU08_00370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ70197.1"
                     /translation="MRIAMFTDTFVPDTNGVAVSSYNLFKALNDRGHYCVVVTSNPFS
                     KEVTFIDNVLRIPGIEMKKLYGYNFAGPNRKAHKILKKLKIDIVHVQNEAGMGIFGKS
                     FARQQKIPYVYTYHTMYEDYTYYATKGRMETFAKAIIRRFSRWQAEIADEFIVPSDKT
                     KVSLREYGVQRYINVIPSGVDFSKFMYNPEDKPFLDEYKKEHGLDGTFNLVYVGRVAK
                     EKSIDVVIRGYALYVKKNLNHNTRLIIVGGGPAIPELEELAEKLQIAPFVHFVGKVLP
                     ADIGKYYHLGEVFVSASISETQGLTFMEAMAAERLLLCRFDEGLVGVIRDGESGFFFR
                     SEEDFGEMVKKIRSLSTKEIRNILDEANNIVDYYSNDRFVERCEHVYLRALRNSW"
     gene            13653..14423
                     /locus_tag="PLU08_00375"
     CDS             13653..14423
                     /locus_tag="PLU08_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOQ70198.1"
                     /translation="MVKIINITLKKEQYNITFDNGEELLVSAETIAEFFLYNGKELSL
                     KDLAKIKEYEAQTKHFSYASNLLARRPYTSHEIREKLRKRRVNEEVIDLIVDKLKRLN
                     LINDLRYALDKIQYLINVKKCSTRAIEQDLLRRGIHPYVIKDALAQSPRDERRELEIL
                     IPKLLKSYRNESLRRAYQKLKTKLYSLGFESDNIDEVLAKFNVEDYIDETENIKKALV
                     KLEKKAKGDTKVLYNKLVIAGYPASLIRKLLEDYSYEN"
     gene            14413..15363
                     /locus_tag="PLU08_00380"
     CDS             14413..15363
                     /locus_tag="PLU08_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ70199.1"
                     /translation="MKIKDLNDGIRVIENYLVTNVTKGVSNNGQMYLTVSLQDNTGQI
                     EARVWDANENDVEVFKQGNFVRVVADVIEYRSNLQLKVMTGEVLNPKEVRLDEFTMAS
                     PVKLETLTAELNRLLSSFADTDIQRLVERLISDRYEAFITYPAASRLHHEYTHGLLEH
                     TITIANLADRIIDYYLELYEGDWHINRDLVIAGILLHDLGKTIELSGPVLTSYTVAGR
                     LVGHISLMNAELLKVAEELEIKSEMPILLSHIILSHHGKLEYGSPVTPMTKEAQLVSM
                     IDDLDAKMKLIEKALNTTNSGEFTDRLWALDSTSFYKPKK"
     gene            complement(15364..15780)
                     /locus_tag="PLU08_00385"
     CDS             complement(15364..15780)
                     /locus_tag="PLU08_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOQ70200.1"
                     /translation="MSKDCVFCKIIRREIPGAIVYEDNDVLAFLDISPTAPGHTLVIP
                     KEHHETFLGTPRKLMYNVMDVAQRIGQVQIKTLKAQGVNILINNYPPAGQSVPHFHVH
                     VIPRYGKKDGLKIAMVEHLDGDLNFPAVVEKIKSGL"
     gene            complement(15882..17444)
                     /locus_tag="PLU08_00390"
     CDS             complement(15882..17444)
                     /locus_tag="PLU08_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70201.1"
                     /translation="MKRKYLLGLAVAALLLTGCDKITAEPVDLDKTVLVDELSEVVNN
                     TLENVYKKYHDSANFKQFVLDEVLLTIAEHEFGPYDKLAADDPFKLAVDTRAKHYFYD
                     ELLSGSYTYRSIFDEKKYVVERVYGTENSYLVDANGNEVAIGAIDTLPFYKEGLFLPI
                     VNKDNFDDPAFKLVHIDYYKHYMQDRYVDKIYREKLTEKFVKEEQTSTLGRNYARKVE
                     YVAIKNNSNHPTAASALVNAFIDNNILGAEPADFNILANAWRGYEPDFIGNEAALLAA
                     TGLKTAEVNKTLIGDIESDFAKIKANPDLTDKAIEDRFTGSGKYPKEVGKQIEINEVR
                     KQSFVTKDWGIKDGGFSSLPEAIRSRLFNIGVANSVDFLKDDAGVLADAGKWEGDAEN
                     KKVASTFVRNIHDDYYLVPKTYEKGNNRNFLFFENDTFYIIKIVEAVNTAKLAAKDDD
                     LNSYEHFKTEAEIAEIVAHVTEALAKLDATKTNALNFYMKDLKIVFHDEDVYDFFKEK
                     FPDIFDDEKKKK"
     gene            complement(17449..19200)
                     /locus_tag="PLU08_00395"
     CDS             complement(17449..19200)
                     /locus_tag="PLU08_00395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ70202.1"
                     /translation="MRKPKRIIAGLALSALLLTGCSKDLVADKKGNIVTVTIDGNPVQ
                     INAGDMFEDYLKSSDGLKDYYDAVYEVVVRALFNKEEQTTERTEIYDIAALSVNDVKE
                     QAKNNAKGGGKYDDELKKILKTKNVETLKELEEKLAYDEMQVRLKKQFFDGGEGAWIT
                     NAMEELIVGERNQAGDLILDENNNPVYEGYLNNKLPYHVRHLLVKVGAANNDFVTGKI
                     SKDDANKLSSVIVALANHQDTFGLLANRYSEDEDSARQFGDLGIMNFTTNFVNEFKLG
                     VYAYDALYNADGVMTPEAKAAKLKIPGTAKTKLDALGLDEIPYGAALKLAAVREIEKS
                     DAGKEVNDGDAAFFPRNVIFNKYFNKHNIGVITPNEVNVLDEVGVEKAEFAALPGFKA
                     LPELGGKKVLTDEKGNVILVVRAGSAGSYEGIHFIVVERSALYDTVNGVTLKDYYTIE
                     TPGTAAFPKDDAGNDIDAFVNFLELDARGTKERIDKIRNEVKAFDPTINDRIFQKLVT
                     YLDVKFHDAELKAALDKYLANQYARSQFQLDQDLDKLWSEWIEYLDQQEVARTDRLVP
                     ETYIDDFKAGNDYNYGG"
     gene            complement(19465..21591)
                     /locus_tag="PLU08_00400"
     CDS             complement(19465..21591)
                     /locus_tag="PLU08_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOQ70203.1"
                     /translation="MKTTLIIPNIGCPNCAMAITEHFKTLNIEAKVNVNQKKVTFTYS
                     DKDSFTIILKELKAIGFPAAPIDYDEKRRRKYEKVRLIVATILVLPLAYTMFDNFGLN
                     IVPDIMLNGYFQMGFAAILQFIFGFRFYKNAFYQIKNKNLGMDVLIVLGTSVAFFYSL
                     YLTIFTDKTQLFFETSGMLIWMVTIGDYLEHLSQAKTTDTLKSLMALAVDEVRLIKNG
                     EEQIVSLNDVNVGDVIKVLAGEKIALDGEIIDGASYIDDSVITGESIPKYLSVGDTAV
                     GSTTNLSNTILIKVSAIGSDTVLAKIIKTVEETSLIKPKFQKAVDVIAKYFVFIILMI
                     ALVSFLVYQFILGKELSISLEVTTAVLVVSCPCALGLATPTSIAVASGLAFKNKILYK
                     GGEFFELANKIDAIAFDKTGTLTKGDLIVSDYLGDIRYLSYTKGLEIHSTHPIGKSIN
                     NYRKDIIPFAIEEYEIIDGKGIKGTYLQDQIIVGSATFIAEANISNPFLNEFEKFTGE
                     GKVVIFTAINKKVVNMIILEDEIKAESQTLITALKKMNINVYLITGDNENTAKYLANK
                     VGIENYYSGVLPNEKAHIIKKLQEKHSVVAFVGDGINDAPALKQADVSFSVSNASDIA
                     TDTADVTLLDNDLKLVLIAIDLSKKTTANIKQNIIWAFLYNLVMIPLAIFNIATPLVG
                     GFLHIISSLLVVFNALRLNLYKHKIK"
     gene            21919..22731
                     /gene="sufC"
                     /locus_tag="PLU08_00405"
     CDS             21919..22731
                     /gene="sufC"
                     /locus_tag="PLU08_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOQ70204.1"
                     /translation="MVLIEIKDLHASVNDIEILRGVNLKINRGEVVALLGPNGHGKST
                     LLQVIMGHPNYKVTKGEILYKGQDVLAMSVDERARAGLFLAMQNPSEVPGVQISDFLR
                     AAINTKREKPIKPIELFRVIDKASKEMQMPHDLASRNLNEGFSGGEKKRHEILQMKLL
                     KPEFAMLDEIDSGLDVDGIRIVSQQINEEKKDKTFLVISHYARLYELINPTRAIVMIN
                     GRVAIEGGMDVIKRIDQEGYEWIQVEHNINIEREDKRPIVLEICAVNERSKQ"
     gene            22728..23561
                     /locus_tag="PLU08_00410"
     CDS             22728..23561
                     /locus_tag="PLU08_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOQ70205.1"
                     /translation="MSVFKALLINKMNINDDTLILSENDTVYHIFDENDLPTSVTKVI
                     LKSSKDIDFVVVLRQNKKFITYELAPYTRGKVIFMCSEENLSIDREIILLEGAEVKLI
                     MPDFHNGNRKVNIETKLSERKARAEWHLATYSQNIDKKVFNISFSHFGNESFADMHNY
                     GVVLNASTLIFTGESTIFENVKGAETHQTARIIVFDENSHAEANPILNIYHNDVVAAS
                     HAATVGQVNAEHLYYLKSRGLTEKEAKLLITKGYLMPILNYIEDKVLKENCITMLERV
                     L"
     gene            23561..24775
                     /locus_tag="PLU08_00415"
     CDS             23561..24775
                     /locus_tag="PLU08_00415"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOQ70206.1"
                     /translation="MYDVYKIRKDFPVFDNNKTIYLDNAATTYKPQVVIDAVTNYLSK
                     ETANSGRGDYQNAYLVDKRVDETRALIHKFINSKHVQEVVFTQGTTASLNMVAKCYGE
                     KFLKEGDEILLSIAEHASNTLPWMQVAKKTGAKIVYMPLSEDGKVTPEIVEKYMTEHT
                     KVVSVLHVSNVLGHVHGIKQIAKIVHDKGAIIVLDGAQSVPHMKVDVQDLDVDFLAFS
                     GHKMLAPTGIGVLYGKKELLEKMDPYQTGGGMNFSYDKEGNYELFKVPLKFEAGTLNI
                     EAIYGLHAAIEYINKIGIENISKHEHKLRTYAVSKLKEMDHIILYNENAETGIITFNV
                     KNVFAQDVATYFNSKNIAVRSGEHCAKLLKEHLREVATVRASLYLYTTKEEIDAFVEA
                     ARTAENYLDAYF"
     gene            24762..25217
                     /locus_tag="PLU08_00420"
     CDS             24762..25217
                     /locus_tag="PLU08_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HOQ70207.1"
                     /translation="MPIFNNELMRATILTHYENPYHKETPQNADEFMQIHLTSSGCID
                     NLTLYLKVKDNKVITGYFDGFGCTISIASTSILLEMIEGMNVDAVLKIIAEYKKMLNS
                     EPYDIDILNEAIVFANTVRQPSRIRCATIGWDGVAELITKHKEEENHND"
     gene            25210..26610
                     /gene="sufB"
                     /locus_tag="PLU08_00425"
     CDS             25210..26610
                     /gene="sufB"
                     /locus_tag="PLU08_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOQ70208.1"
                     /translation="MTSADELKFGTYAYGFSDDQESIASTGIGINEDVVRQISAFKEE
                     PEWMLEFRLKSLRAFELMKNPKFGPSLKDLDFDSYTYFNRVVKDEARSWDDVPDTIKE
                     TFEKLGIPEAERKFLAGVATQYDSEVVYASMISEFKEKGVIFSSTDEALKEHPEIFKK
                     YFNTVVKYTDNKYTALNGAVWSGGSFIYVPKGVELDKPLQSYFRLNAAKMGQFERTLI
                     IVDEGASVHYVEGCTAPVYSEDSLHAAVVEIIVHKDAKCRYTTIQNWSNNIINLVTKR
                     ADVYENGQMEWIDGNIGSSINMKYPACILRGEGARGTTISIAVAGKGQYQDAGARMIH
                     LAPNTSSTIISKSVVHSGGTANYRGLVRHTKNAPNSQTHIECDTLILDGISKSDTIPT
                     NEIHNNESFIEHEATVSKISEEQLFYLMSRGLSEEDATQMIIMGFIEPFSRELPMEYA
                     VELNRLIKLDMEGSLG"
     gene            26610..28493
                     /gene="mnmG"
                     /locus_tag="PLU08_00430"
     CDS             26610..28493
                     /gene="mnmG"
                     /locus_tag="PLU08_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOQ70209.1"
                     /translation="MKFDYDIIVVGGGHAGVEAALASAHMGKNTLLLTMDIKKIANMP
                     CNPNIGGSAKGIVVREVDALGGMMGIFADHDPLQIKILNTGKGPGVRCLRAQEDKVGY
                     PRYVQSIVLNTPNLTVKEAMVVSVIHDTEKVYGVRLENDEEILAEATILTTGTYMEST
                     ILVGHTVKEIGPEGDRSSKGLSPYLEKMGLKIFRLKTGTPARLKKDTIDYSVMVEEPG
                     MEGYLNFSYMSERYHTLEEQVLCHLLYTTPETHAIINEHLGDSAMYSGLVTGTGARYC
                     PSIEDKIVRFSDKERHQLFIEPESRYTDSMYLQGLSTSMPVEVQEKMIRSLKGLENVE
                     ILKYGYAIEYDAIDPLELKPTLELKKWDNLYCAGQIIGTSGYEEAAGLGLLAGINAVL
                     KIDNKPPLILKRDEAYIGVMIDDLVTKGTEEPYRLLSSRSEYRLLLRHDNADSRLTEI
                     GRQVGLVKDDRYAKFLKSQKAIADARAILETTQVSADIPVVEFLESVGYPNYSGGLTI
                     AELIKRPRVTLDDLLELLGHLTLEGLNHNDLFQLETDIKYEGYINKQRQEAKRLKKLE
                     EVEIPASFTYDDLDGLRIEARQRLAKVKPLTIGQAKRISGVNPNDIIILMTYLRRYGK
                     NEK"
     gene            28483..29184
                     /gene="rsmG"
                     /locus_tag="PLU08_00435"
     CDS             28483..29184
                     /gene="rsmG"
                     /locus_tag="PLU08_00435"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOQ70210.1"
                     /translation="MKNNPFLQYLDEHFSGNAEVLKVQFAKYAALIEEHNKVQNLTAI
                     APSEYEEKHFLDCLLLRETYNFRNETLLDIGSGAGFPGIVLAIAYPELQITLLEPIKK
                     RCNFLSTVVKELELTNVEVVNERAEDYAASKRGEYDLVTSRAVSSLSIMLELSVPFLR
                     INGSALMMKGRNYNAELSDAENAIYTLKVEVGKTDLHFLPSDGSMRVNARFIKKGSTP
                     KKYPRPFAQIKKNPL"
     gene            29218..29997
                     /locus_tag="PLU08_00440"
     CDS             29218..29997
                     /locus_tag="PLU08_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003323489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOQ70211.1"
                     /translation="MAKIIAIANQKGGVGKTTTAINLSASFAHFGRKVLLIDMDPQGN
                     SSRGLGLDPATIKNTIYHTFFKGININKAIYRTPLKSLDIIGANLLLARTDVEISTIP
                     QHPFTVLQSKIKEIDRRYDYIFIDCPPSLGLLSTNALVAANSVIIPVQCEYFALEAVA
                     QVLATIKQVQASHNSELKIEGFLLTMYDERTRLGIEISQQIRGLFKESTFMTPIPRNI
                     ALAEASAKMLPTILYKPTSKGSLAYLNVAKEIMENEERAEE"
     gene            29975..30781
                     /locus_tag="PLU08_00445"
     CDS             29975..30781
                     /locus_tag="PLU08_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOQ70212.1"
                     /translation="MKNEPKNNLLKEHLSDLISKYSQSNVLEELAKAHTNRPIQMLPI
                     DLIVDNNFIKKITLSRKKIARFVENYQRNILLEPLVVRAKGDKYEVIIGRRRLAAARL
                     TEIQEIPVIVMNYNDEETLLILLAKARDQLGTNVLELAYIFSVLSTKHGYSHQALADL
                     TFRSRSQVTNILRLLKLEPEVIRALNNEQITFGHARALISFDREKSLEYLELIRVNNL
                     SVRELEALIKAPNTATYNGKDYFVIQMKNKLTIVFNTEEEALAFKNKHFK"
     regulatory      30821..30933
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            30972..31631
                     /locus_tag="PLU08_00450"
     CDS             30972..31631
                     /locus_tag="PLU08_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOQ70213.1"
                     /translation="MEKEKNYTNCQNDNLETNAQQKCKRRRTVTVAGTAIFAAISVLL
                     YITKVPPFVFPIFPLVNFLEINFSEIPALIAGFAYGPLSGFLVLLVRFLVKLPMSSTA
                     MVGEVADIIYSSSFVLTATIIYKYRRTFKGALIGLGIAFIAQILVSSLANYFVIYHLY
                     NEMFFNGKMAMILSNKYGAFTPEQFILWVIPFNFIKNTIIGGLTLVVYKRISRFINQF
                     Y"
     gene            complement(31686..31880)
                     /locus_tag="PLU08_00455"
     CDS             complement(31686..31880)
                     /locus_tag="PLU08_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOQ70214.1"
                     /translation="MAKKVKLDKDLCISCGVCNAVCPSLFDWDDDGKLKALVEEVPAD
                     LEDAAAEAEASCPTGAITVE"
     gene            32005..32325
                     /locus_tag="PLU08_00460"
     CDS             32005..32325
                     /locus_tag="PLU08_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70215.1"
                     /translation="MAKRRESKTRVEYWAKYRESITNDFEVEQKMRESRKKQAENTPI
                     KKTETPPLTKAEALLSEYERKNENKESAVKKGPIGILYGLAIIVLLLIIAGIIFMILI
                     YGNK"
     gene            32312..32986
                     /gene="cmk"
                     /locus_tag="PLU08_00465"
     CDS             32312..32986
                     /gene="cmk"
                     /locus_tag="PLU08_00465"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOQ70216.1"
                     /translation="MGINNIAIAIDGPAAAGKSTIAKKVANHYNFVYIDTGAMYRAFT
                     WYVLKRGVDPKDETASNSLIPEVTIRLTADYKVFVNGEDVSKAIREPSVSGNVSYIAS
                     YPKIRLFLTEQQREMAKVNSVVMDGRDIGTYVLPDAFVKIFQTASVAVRAKRRYLENL
                     EKGIATSLEDIEKELAMRDHIDSTRAMAPLKKADDAIELDTDNLSIDEVVAEIIKIVD
                     KKLGVK"
     gene            32986..34293
                     /gene="der"
                     /locus_tag="PLU08_00470"
     CDS             32986..34293
                     /gene="der"
                     /locus_tag="PLU08_00470"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOQ70217.1"
                     /translation="MALPVVAIVGTPNVGKSTVFNRIIGERRAIIHDERGVTRDRLYG
                     SAEWLTRRFNLIDTGGIEIAKTAFQEQIRAQVEIAIEEADVIVYVGDGPLGVTTDDRE
                     IANLLYRANKPVIVAVNKIDGIEHHHYIHDFYSLGLGEPIAISGEHGIGIGDLLDEII
                     KVLPEQKDDYEEDVISFAVIGQPNVGKSSLVNAILNRERVIVTNVEGTTRDAIDTPFT
                     RNNKNYVVIDTAGLKKRGKIYESVDRYAALRAFSAIAKADIILFVIDAVVGIREQDKH
                     VVGYAVDEKKAIIIAVNKWDAITKDDKTMNEWTNKIRNEFKFVEYAPIVFVSALTKQR
                     VDQIFDQIDYVNEAYRRRVETSLLNEVIHRAQLYNDPPVFNGGRLKVYYANQVSTKPP
                     TFVFFVNNPAFLHFSYERYLENQIRDSFPFDGTPLKFIFRERK"
     gene            34450..34722
                     /locus_tag="PLU08_00475"
     CDS             34450..34722
                     /locus_tag="PLU08_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOQ70218.1"
                     /translation="MNKAEIIAAIADELMITKRSVEEIINLFCDTVIETLAKGEDVKL
                     TGFGTFQTRERKERRGVSPQTGKEIVIPSGRTIVFKPSKLLKEKVQ"
     gene            34744..35406
                     /locus_tag="PLU08_00480"
     CDS             34744..35406
                     /locus_tag="PLU08_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70219.1"
                     /translation="MLKSYLEFFDEIANIFNKHGFKLYMVGGTSRDFLLNYPVDDYDF
                     ATDATPTEMHEFLPDANYRFEKFGTVSLKYEDEHVEITTLREEGEYIDSRHPAYIKFV
                     TDPAKDYIRRDFTINALYIDENYRVLDFCDGLKDLDTNTLRVIGNPYTRFAEDPLRII
                     RALRFVAKFKLNIDPLTENAIRDLAHLVKKLNPEKVKAEIRKVGEQEQNIFKKLMNMY
                     KL"
     gene            35461..37248
                     /gene="glmS"
                     /locus_tag="PLU08_00485"
     CDS             35461..37248
                     /gene="glmS"
                     /locus_tag="PLU08_00485"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOQ70220.1"
                     /translation="MSGFIGRVGNSCVKEKLLSALNLLQFKDDDSTGVLFCNKEGQHL
                     YRVVGKVDELVTLIPEEVDGSMGIAHTRWSTYMHQVLENVYPLSSENGYVTLVVNGLI
                     DNIQMIKRRLVKRGYSFRSSSAVEVVANLIEDGAVENEDPLRTLHRAMQQLEGDYALL
                     AVFNGNCKKIFFAKNGVPLIIGKNHDGMVICTDYTPIADQADEYYYPNDGELGYLTSD
                     NMVLYDGKLKQRKPSFTEVSVSEKTLELGDYPHYMLKEIEEAPGVIRRLINHYFDGTK
                     YRFDDRLIKRIIDAGTIVFIAAGTSYNAALVGQRYLRNFNKKVDVFIASEWIFYPYQS
                     GEDTLYVLISQSGETADILKCLRTIRAYGADVLAITNVEVSTLYQSADYRLLLHAGTE
                     VSVASTKAYIAQITLLSLLYARLVNKITTIAALERVIEALYNIISRRDTIKAIADQIY
                     KSGDVFFLGRGFDYDVAVEAQLKLKETSYIHAEAYPGGEMLHGPIALIEENTPVIFFI
                     SDSLTVAATREVIREMRDRKANVIVCSLAPFNHDGDSFIISNPIKGYHSPITFAVFAQ
                     YLAYYCAVNLGRDVDKPRNLTKAVKDRSV"
     gene            37271..37888
                     /locus_tag="PLU08_00490"
     CDS             37271..37888
                     /locus_tag="PLU08_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOQ70221.1"
                     /translation="MLFEYYKELRINETELVIILFIEHLQQKETEFITPDLINLQSNI
                     DIKVIDEAMVRLVSKGLIEFTTRNGKMVTSLNPLRNKLQELFVLDYEKNKENSTVEFS
                     SQVEEVYAFIQNAFGRSLSAVELQTINDWFNYGYNITMIKEALDQTMKRKRYNIRAID
                     KALLKLSTAVDYALEGKSAQSDSYRKNIKDTLEEVNKKAQDDENE"
     gene            37872..38507
                     /gene="nth"
                     /locus_tag="PLU08_00495"
     CDS             37872..38507
                     /gene="nth"
                     /locus_tag="PLU08_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOQ70222.1"
                     /translation="MTRTNKERILAFLDKEYSDAKGELEYTKDYELLIAVVLSAQSTD
                     VAVNNVTRKLFAKYPTFESLLNVKEAQFAAYFKTLGLYRNKAKHVYNLIHLLNEHHSG
                     VVPSAKDELLKLPGVGVKTANVVRAELFNIPEIAVDTHVSRIARRLGFAKFSDDVTII
                     EAKLRKVLPKERYIKTHHQMIHFGRYFCKAQSPLCKKCPLVDICKEPNKKL"
     gene            complement(38502..39092)
                     /gene="recU"
                     /locus_tag="PLU08_00500"
     CDS             complement(38502..39092)
                     /gene="recU"
                     /locus_tag="PLU08_00500"
                     /inference="COORDINATES: protein motif:HMM:NF002581.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HOQ70223.1"
                     /translation="MINYPNGSKPKKQTTKVPSKRKNITKSAANRGMALEKLISAAND
                     YYLMHDIALFTKRPTPINVVKIDYAKGAKITNAYFEKQSTTDFNGVYKNRYFDFEAKS
                     TRLKTSFPLNNIYEHQITHLENVIRHGGIAFFIVEFTAFDEYYVLSAADVIDFYRNGD
                     RKSIPYASFKEKGVLVRLGLNPVLDYLPAVIKLFNL"
     gene            complement(39092..39817)
                     /locus_tag="PLU08_00505"
     CDS             complement(39092..39817)
                     /locus_tag="PLU08_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOQ70224.1"
                     /translation="MKKLAKATRGRKQLIFLDLEGTQFSHELIAIGAIKATIKTDGTI
                     KKLYPGFKHYVLATQNVGNYIEKLTGITDDLLAREGIPFDKILLMLKKYVGTNLSKTK
                     FVTFGAHDIRILRQSLAHTPEAHHTFIKTIAKNHIDLSAYINEYIKDEKNNTLSLVNL
                     CKLFGVEPVEPAHDPLNDALMLAYLYSELFKKTDILIERYGEVLLNMNNIPRPIKRML
                     NKIKAGESVNIDDLQEYLRDEIV"
     gene            39909..40259
                     /locus_tag="PLU08_00510"
     CDS             39909..40259
                     /locus_tag="PLU08_00510"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOQ70225.1"
                     /translation="MAEKRLLDADKILKKKFKAKSGGYDALEVDEFFDLVRNDYESML
                     EIEKELELLRLKNETQQAKIVNLEAQYIQYKKKVEELERLISKGGTAMENLRKIDKYE
                     RQLWKMGIDPSKLK"
     gene            40264..40597
                     /gene="rnpB"
                     /locus_tag="PLU08_00515"
     ncRNA           40264..40597
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLU08_00515"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            40622..41260
                     /gene="pgsA"
                     /locus_tag="PLU08_00520"
     CDS             40622..41260
                     /gene="pgsA"
                     /locus_tag="PLU08_00520"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOQ70226.1"
                     /translation="MNLPNKLTVARMILVILLIAFLLIPPTWLGNEPIILFSNVYLRY
                     FIAAIVFIVAAITDYFDGYLARKFNLITNFGKFMDPIADKMLVNSMLIILTVPHQAFV
                     GQISVPVIFVVLMIARDTVVDGFRLVAANQNKVLAANIFGKAKTVLQMIAIVMFLFND
                     WPFSHQMQSDSFPIVSMIVIGAATFVSLLSGFIYIFQNISVLKETHDDTTAH"
     gene            41238..41711
                     /locus_tag="PLU08_00525"
     CDS             41238..41711
                     /locus_tag="PLU08_00525"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOQ70227.1"
                     /translation="MTTQLLINELIDKNLTIGSVESFTGGLFAATLVNYPGVSKTYKG
                     TIVAYSNDVKERLLNIKKEDLKKFSAVSSVVALDLARHGQKALGVDFCFGFTGDAGPT
                     ASGQSGEVGDVYIALAFANDEIVEHFKFKGDRLKVRTDAVNAALKMLARVLKNFE"
     gene            41745..42746
                     /gene="recA"
                     /locus_tag="PLU08_00530"
     CDS             41745..42746
                     /gene="recA"
                     /locus_tag="PLU08_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOQ70228.1"
                     /translation="MSKKKDANILQETLKEIEKSFGKGAIMKLGERPVINVDVIPSGS
                     LLIDEALGVGGYPKGRIIEIFGPESSGKTTLALHAIAECQKKGGQAAFVDAEHAIDPI
                     YAKNLGINIDELLLAQPDSGEQALEIVVRLAESGVIDLIVVDSVAALVPEAELSGEMG
                     DNQVGLQARLMSKAMRKLAGVLNKAECTVIFINQLREKVGIIYGNPEVTSGGRALKFY
                     SSIRIDIRRGEAIKQGSKLIGNQAKIRIVKNKVAPPFAACGIDIIYGKGISKIGEILE
                     LAVNRGFIKKSGSWYEHDGLKIGQGREAAQNYILERPELLADLEEKIKTGVVATETK"
     gene            43040..44590
                     /gene="rny"
                     /locus_tag="PLU08_00535"
     CDS             43040..44590
                     /gene="rny"
                     /locus_tag="PLU08_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004801659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOQ70229.1"
                     /translation="MEVLYYALFFLVGLGLGIGLFFLIPAFKAKQAFAKAQKLTKEAE
                     IKAEHIVKNAELDAKQIIYEGKQELEQAGKERRAQIRELERLLNDREKGIERRDQLLH
                     SKEQSLDARHENLSVKIKEADELKKDLQIKIDSILGELETVAQMTVSEARDELYHRVD
                     AKLEKEIALYIKQKEEEAELQVNEQARDILANTIARYSQEVTTERTVSTVELPNDEMK
                     GRIIGREGRNIRTLEQLLGVDILIDDTPEVITVSCFDPIRRETARRTIELLVKDGRIQ
                     PSRIEEVVEKATQELDEIIIKTGKDALAELGIPNVHKDMIPFIGRLKYRTSYGQSALK
                     HSIEVAHLCGIMAAELGLDITRAKRAGLFHDIGKSADFELEGSHVEVGARLAKKFNEP
                     DYVIDAIESHHGDKESRFIFSQLVIAADTLSAARPGARTETLENYIKRLEKLEEICKS
                     YDGVQTSFAMQSGREVRVMVHPDKVDDIMVNKLVRDIRERIENEMTYPGQIKVSVIRE
                     YRASDVAK"
     gene            44593..45390
                     /locus_tag="PLU08_00540"
     CDS             44593..45390
                     /locus_tag="PLU08_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOQ70230.1"
                     /translation="MVKILFIGDIVGKIGRETLAHFLPILKREHDFDLIIANGENTTH
                     GRGLTKKHYEELVNLGIDVITLGNHFDDRPELKYYITEVHNLVRPLNLIENYPGEGSV
                     VVTTKKGDKVRVTNVLGQAFMKEIITSPYANLKMLNADTEKMIHIVDYHAEATAEKMT
                     IGWEFDGAVSAVLGTHTHVQTRDARILPKGTAYISDVGMTGAYNGIIGADRERVIERT
                     WFDKQVPFTYEYKDKEAQFSAVILEINKHTFKTEKITPLYYLWRRDE"
     gene            45383..46819
                     /gene="miaB"
                     /locus_tag="PLU08_00545"
     CDS             45383..46819
                     /gene="miaB"
                     /locus_tag="PLU08_00545"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOQ70231.1"
                     /translation="MSKTLLNNLPSFTKARRRYPSIVQNLKIEVPSIVSAKLSGKFFL
                     IKTFGCQANVRDEESMAGLLSMANMRRTEEEKDASVIIINTCAVREGAEQKIFGQVGN
                     LKGLKTNNKDLIVIVAGCVVQQEESLAKLRKTFPYVDIFMGTNAIHKLLDLIALRITE
                     NKKVVDVPSDDADVYENIPVQRIAPHKAFVNITYGCDKFCTYCIVPYTRGKERSRLPA
                     DILRECQILVDSGYQEITLVGQNVNAYGIDLKDGTTFSYILEEVAKLGIPRLRFITSH
                     PWNFDDEMIDIIAKYPNIMRKIHLPLQSGSDEILKLMGRRYTVKDYLELVTKLRARMP
                     DISLTTDIIVGFPNETEQQFLETLKVVEKVNYENAYTFIYSPRKGTPAALMEDNVSRE
                     EKGERFNKLKALVDSQTEANAALYVGNEYLILVDGYSKKDTSVLSGYTESDRVVHFVG
                     TPDLIGKIVKVKITESRVYSFVGELVNE"
     gene            46812..47123
                     /locus_tag="PLU08_00550"
     CDS             46812..47123
                     /locus_tag="PLU08_00550"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HOQ70232.1"
                     /translation="MNKVEIALNALTTELANDKRVVEFKKVKALIESDAYLKNVEVRL
                     KELQRLMTQNAFNEEKHNEYKREYLRLKNNYETHPYLINYNSLLSEIEDLLYSLKTVI
                     E"
     gene            47155..49686
                     /gene="mutS"
                     /locus_tag="PLU08_00555"
     CDS             47155..49686
                     /gene="mutS"
                     /locus_tag="PLU08_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOQ70233.1"
                     /translation="MGKEKKKIVYTPMIEQYLEIKRENPGILIMYRLGDFYEFFFEDA
                     EIVSKELQLVLTKRAAGNNRKIPMCGIPHHAYGPYLAKLIDAGHKVGIVEQMEDPSSA
                     KGIVKREIIQIVTPGSIVDFEDSDNNFITAVDAYADAFTVSYADVSTGDIFVTTLPRE
                     DTSLVSYLNGLNTREIVVSPQFSDKLLGVIKTNINLVISFEENTKIDEEYEDLFSDVK
                     DEREYLIAARLVSYLTRTKKRQLSYFKAITVTKPQSFLQIDAFSRLNLELTRTIRNET
                     KYGSLFWLLDETTTAMGRRKLKDFIIRPSHDYDEIIRRQNIVNTFIVNFVTRAEVKEH
                     FVSIYDLERIVARISFGSANPRDLLCLKTSLQALEKARNLLGKIREPEISELYDKLAS
                     NIPLITLLEKAISEDAPLQITGGEIFKYGYYEALDELIDLAHGGKKWISAFEEKERVR
                     TGIKNLKIGYNRVFGYYIEVSNSNLDQVKDEFGYIRKQTTKNGERFINEALKEQETLI
                     LSAEERRQQLERELFGALLNELKKSTRLLQQSADVVSEIDVFISLAEVAVANNYVRPI
                     FNDARVVDIKEARHPVLSKLLKDHAFVANDIELDEATDIVIITGPNMGGKSTLMRQLA
                     LIVIMAQIGSFVPAKSANLMIFDQIFTRIGASDDLISGQSTFMVEMIEANYALRKATP
                     NSLLLFDEIGRGTSTYDGMALAYAMLEYIANKLKVKTLFSTHYHEITAIDEHYPNIKN
                     IHVEVKEVENNVTFLYKIKPGPMNKSYGINVARLAGLPLEILSNAQEHLQTFVANNSG
                     GNPTKTEYVPLKPLWYQQLENLNIDEMTPLDALILLKKIKDEGFK"
     gene            49686..51440
                     /gene="mutL"
                     /locus_tag="PLU08_00560"
     CDS             49686..51440
                     /gene="mutL"
                     /locus_tag="PLU08_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOQ70234.1"
                     /translation="MAKIILLDNELANQIAAGEVIERPSSVVKELVENSIDAGATKID
                     VFINEAGRKAIIVQDNGSGIPSDELALAFSRHATSKIHSTFNLFRIRTLGFRGEALPS
                     IASIAKVQVETAEKGKPGALAKIENGDIKIEPSAVSSGTKITVSELFYNTPARLKYLK
                     RDYTENAVTLDVVTKIALAHPDVSFSLTFDDKLRFRTSGRNNLEETILNIYGLTYVKN
                     LLPISVKTNDFTITGFLGNTNLVRSNRYGIITNLNGRNVYMPKVQSTIIEAYHGFIAP
                     TRYPFVILNLQIEPELVDVNVHPTKKEVRFSKEEDLRNVLLKEIPLALRKEAFIPKDP
                     HQKVQPLSSTTNEEPRAEQLIFDLPFGTETSELKISDSHHQEAIRKPITKTISELENS
                     FVNPVAALHNTYIICEVNDGGFWLIDQHAAHERINYEKFQKVLNMDLKTRPLLVPELL
                     SFSHSEIALFTPEVFTILKDVGIEAELFGPNTLRVTAVPSWAGEYDERIYIQDIIDAV
                     ITDKRLNQELLRTNAIATMACKASIKANHRLSIMEMQHLIKELLACDNPFSCPHGRPT
                     IIKFTKYELEKMFKRTGT"
     gene            51437..52318
                     /gene="miaA"
                     /locus_tag="PLU08_00565"
     CDS             51437..52318
                     /gene="miaA"
                     /locus_tag="PLU08_00565"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOQ70235.1"
                     /translation="MIFVIGGVTASGKSDLAFRFAKAINGIIINADAFAFYKELNIGT
                     AKPTKEELAMLPTFLFNNISLDERFSIYNYQKEVRIILDKYLAKKIPIVLVGGSGLYI
                     RAALYNYNLQPEKDLKIVDDPNISNTSLHKELEVLDKEAAAKIHPNNRKRVLRALALI
                     KTHAQTKTALEEVTSSEPLYPYIMICLDKENEMLHRDIETRVKLMFDKGLREEAMTLS
                     KKYPRNAQGMQAIGYKEIVQNPNATDEELINLIALRTRQYAKRQRTFFRNQFAAKWFT
                     DKEAAFNYLRGKYEDSL"
     gene            52315..53919
                     /locus_tag="PLU08_00570"
     CDS             52315..53919
                     /locus_tag="PLU08_00570"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOQ70236.1"
                     /translation="MKNTLHILEVAKKVGLSPADVILYGEDKAKIKSPFTDINPQGKL
                     ILVTAITPTPFGEGKTTMSIALQDAMWKTGTKSLLCLREPSLGPVFGIKGGATGAGRA
                     KLYPSDDINLHFTGDLHALTSSINLIAAVIDNHIFQGNALNLDPKRITWKRSLDMNDR
                     GLRKVRINQGTGNGTPRCEEFQITVASELMAIMCLASDPDDFIRRVKDIIIGYTYEGE
                     IVRLAALEIEDAIMHLMKQALLPNLVQTLEGNPVLVHGGPFANIAHGCNSIIATKYAL
                     SKAPVVITEAGFASELGAEKFFNIKCRTANLKPNLAVVVATIKALKMHGGVPMSAIYE
                     ENTDALAKGFENLVQHYENLSLFGVKVLVALNLFDNDHKSEVKLFEKEMQKLGYDYAL
                     VSSVSKGSEGGIEFANKVKALLAEETKFEFLYDTEKEVEEKIEIICKKLYRASGVKYS
                     KRAQRELAVIKEQGFAKLPVCMAKTPLSFSDNPNLKNAPRNFNITIREFRLSAGAGFI
                     VALTGKVLTMPGLPKVPAAVNMKEVR"
     gene            53919..54137
                     /locus_tag="PLU08_00575"
     CDS             53919..54137
                     /locus_tag="PLU08_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOQ70237.1"
                     /translation="MALIKVDLYDEIEMKKPHPCIHKSHRFQVVMLGADLKLQCQGCG
                     NIVLISRIKFNQKFKKHIASHETIIFTK"
     gene            54211..54789
                     /locus_tag="PLU08_00580"
     CDS             54211..54789
                     /locus_tag="PLU08_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ70238.1"
                     /translation="MFSNLSYTFKSGKIYVIVGYSGSGKSTFLHIVANYISKNSGKIK
                     YKNKLITKNDVTLVLQHPYFLGDLRIIDNLKISSLFRHLNNRKIRQKYTKALQISHIL
                     QRRANVCSGGEKARANILRGIIERKHILLIDEPTAHLDFKNSQIVAKLLSELSANKIV
                     IVTTHQPSLFKSGNTIFLRIKNGVFHEDNNII"
     gene            54767..56023
                     /locus_tag="PLU08_00585"
     CDS             54767..56023
                     /locus_tag="PLU08_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70239.1"
                     /translation="MKIITLFKVIANLYKRNIKKNRLMVIFMIFTNITLMLFLVFFSS
                     SETLAQTLAEGYLEYLSVDVALYESQPTNNPLINVKSYRRPSIDYLNSIMKVTESFVI
                     RPDYSPLLYEKEIMVLGKEIPNVLITIIGENTNEIGINRAFYNELNKLVSIKDDEMLL
                     HFYIKTTIFIDDEEVLIDYYNQFPITYIYDEPTYFSVPKLYISQNTIDATLGNIFLKP
                     GLTLNNYLLNLNPEHELSNYRFKCHFKSAAQKDKFMHIVNEVSNNESGIEVSGDHINK
                     IESFNALYDYLSILMKLFLAFVIIGSVVIYVVIAHTALLTILRQMALFAVLGASKKQI
                     YEIYLFLIIINFLLSSVSFLLLPSLLPVVSFILHKLMGVYVVLELNYTMLVLMLSADL
                     LTLLILITIIFLINMRRPLLYLLIDD"
     gene            56016..58718
                     /locus_tag="PLU08_00590"
     CDS             56016..58718
                     /locus_tag="PLU08_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ70240.1"
                     /translation="MISIKNLECVLGGERILNIASFEINAPGLYVILGPSGCGKTTLL
                     NIITGLNSEYTGSVELFNRTYRHASKKETTTFRSRYLSAFFQHSVFIEELTMRQNISL
                     VALNHTKSLENLVNSKVEKLAKRLNIEKVLNQKVKTLSGGEKTRGAIARTLLKEVPLY
                     IFDEPTAALDRENAVIVMDLIREYSKTNIIILVTHDESLANEYADTIFTMEYGEFKSV
                     NIIRNNELRVAKALPEIPYAKNSDYIARGLLKAKKARHFFTGGAVNLGLVGLGLSFML
                     VNAINYKLVNTFKGEFTNETVYVQKKFAPEINVLKSVSHEEIALNISAEKELGSFYLN
                     NFNDFFPHQNELSFNHLGIRHVLPSFHIGLLNESLYINEIHEQLYPYVTNLAIDEIGL
                     VLPFDDFKLIQNVLRIPMRQTPSELGVYLENNNFYVTLEVTNNNWDYFDEQIFRLRTV
                     TLGREAQIIMGEPSFIPFIFEEKMMFPSSLNLTKIEEYPWTLKKINYMLLNNAFDYLW
                     QSLNYSNYVFCTANNDYFQYIYNKTYLQNRLLIFESPPHFNSMFKSYSSESEAEFYTF
                     SNGLTFIPEMMMLGFAHNFFITQDITLLEAAMWADINDHSAPSSYLELPEGILNFAIQ
                     YNGFSSFNYRNANTPLRIDELIISSGLANKLGLSKDAHNTELHIGALVAIAEASEGYL
                     KEYETIKVSIKRVIDNDEVVIYHHPLWAYLLYKDVFNVFPFALEITGAIYQSVTNEDL
                     KNLQKHFSVSYPYKDFSVLVADTLNELERYTFLASVGAFLLAALITFMTIYLLVAETS
                     DQFSGLFLVGYNEEAIHGVISRYILRFLGVIILLAIVQLFILSFVVEVVLMKYLETSF
                     RYVFNPKPYLLVLAFATGLLIFLLTYFKFQHEKLDFLAFSKRDL"
     gene            58833..59030
                     /locus_tag="PLU08_00595"
     CDS             58833..59030
                     /locus_tag="PLU08_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOQ70241.1"
                     /translation="MKGTIKQFNQERGYGFIRAEDGTDVFFHYSSLVMDGFKTITAGE
                     KVEFTLEQSDRGPRASNIVKI"
     gene            59134..60249
                     /gene="ychF"
                     /locus_tag="PLU08_00600"
     CDS             59134..60249
                     /gene="ychF"
                     /locus_tag="PLU08_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOQ70242.1"
                     /translation="MGLSVGIIGLPNVGKSTLFNALTASKVEAANYPFATINPNKGTV
                     RVLDPRVTKLAEYYEPEKTIYATIEFNDIAGLVRGANRGEGLGNQFLGHIRECDVILE
                     VVRCFSGEEIIHVEQSVDAKRDIEIINLELILKDLEVLYRRKDKVETKARVNKDKDSV
                     FELEIINKLIPALENEVPARLVTTLTQAETDYVKLHFGLLTIKPLIYVANISDTEYAT
                     YEDNVEYQKVKAIAKREGAELVAISAEIEAEIATLSSADQELYLAELGINESGLNKVI
                     NVAYKTLGLSSFFTTGKDEVRAWTFKDGTSAQKCAGIIHSDFERGFIRAEVYSYNDFE
                     KYGSEQKIKEAGRVRSEGKDYVMRDGDIVFFKFNVRK"
     gene            60626..61102
                     /locus_tag="PLU08_00605"
     CDS             60626..61102
                     /locus_tag="PLU08_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOQ70243.1"
                     /translation="MPKYDGATPTRPDDDTYTYSFNGWDPEPVPVVADATYVAKYKTA
                     LKEVVDPRDFVFADTGEGYTLCGYYGSASRVVIPETYNDKPVVSLEKWAFFNLDNLVS
                     VTLPNNLRKIGEDVFADCDNLKHVDFGNGVQEIGFMAFDHCPSLEAISLPDSVETI"
     gene            61145..63175
                     /locus_tag="PLU08_00610"
     CDS             61145..63175
                     /locus_tag="PLU08_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOQ70244.1"
                     /translation="MSANIKTFGLGVFNKSPNVEYKVYENGLYLGNSTLSEHLLLVEI
                     IDKEQTTFKLHPDTRTIAEFACYKSNMTEVTSWGNNLKTIGYGAFMQSKLTAITLPNS
                     LETIADSAFKQTKLTSLNFGTGLKAIGYEAFSNCPDLADVTLPNSLERIDSSVFYGTS
                     VTYTDYENGRYLGSGDAGETANPYFALMEVITKTMTTFTVHADTHLIVPGVFYGSSSL
                     ESVEIKEKVSFIGSGAFENCIALTSLSVAANNTHFYSPSNKAILEKDTDRLLFGLNGG
                     FIPEDTKIIGEKAFYYRKFTEPLVIPSSVTTIEDYAFYQTNLTAIVIPDSVTKIGKSV
                     FQYSNSLTSVTLSKNLKVIEDWTFSGCKILETLTIPDSVTMIKEWAFSNCEMLANVTY
                     SANLAFIGDVAFSSCVALTTLPASESLRVFGSGSFRGCESLTSITIPKNVRYISSGAF
                     ADCTALTTLNVVSENNHYDSRENCNGIIETATNTLMVAVKDTVIVESIEALGAEVFSG
                     IDIQSITIPASITKIGNWAFSDCGKLETVVIEGELTMINAGTFSHCTSLTSVNIPETV
                     TEIGGSAFYNCKVLPEIKLPANLEKIGSWCFGRCTILPQIVIPRSVTNIARSAFSSCA
                     NLTIYAEATALPDGWHADWNDGNRPVYWYSETANYDGSHWRYVDGVPTVWAE"
     gene            63313..64932
                     /gene="argS"
                     /locus_tag="PLU08_00615"
     CDS             63313..64932
                     /gene="argS"
                     /locus_tag="PLU08_00615"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOQ70245.1"
                     /translation="MIDIRLQELIKDATVKLGLDIDQDTIVIETSKDSAHGDYATNIA
                     MQLARPLRKNPREVATLLINAMDQTPFEKIEIAGPGFINFFLKPETLSKYIATILKEG
                     SNFGNGSKKDTKVNIEFVSANPTGKLHLGHARGAAVGDTLARLFIKSGYEVIREFYVN
                     DAGVQIDNLGKSLYVRYLNLFGIEKELPKDAYHSTDLIAIAESLKAEIGDKYVKESKE
                     GLEYFKLEGTNKFLDIIKKDLQNFDVVFDVYSYETKVRAGNTIPKLLEKLAPFTYEED
                     GATFLKTSDFGDDKDRVIIKSNGDFTYFLPDIAYHLNKLSRGSSLLIDVLGADHHGYI
                     GRMKAALQMFNYPADTLEVQIIQLVRIIQDGKELKMSKRTGNAITLEELCEEVGVDAA
                     RYFFLAKSASTHFDFDLDLATESSSANPVYYAQYAHARLSAVLARGEKFSLDPSGEGL
                     KEAAELNLIKQLIAYPSAVSDATMTREPYKIANYIQKLASLVHSFYTEVRILDESNVQ
                     LTSARLGLSIAAQIVLKDALTLLGVSAPQTM"
BASE COUNT        21691 a        10177 c        12758 g        20365 t
ORIGIN      
        1 gacttaggat ctagtgggga aacccatgca ggttcaattc ctgtcgtttg caccacgtta
       61 cttaaattgc tccttaaacg gggcattttt tattaattcc aatagcgtta gtgataaaaa
      121 agtgataaaa taatgtgtaa atagtttaaa taggagttta tcatgattaa ttatgcccaa
      181 aagataagag agtatcgtga acgaaagttt ttgacacaaa cagaattcgc ggcgcttctt
      241 ggtgtttcta tcgcatcagt gacgagatgg gaaacaggac attatcagcc aacaatgaaa
      301 gttaagaaaa aaatatacaa tttattagtt gaggctggaa ttgaaataga ataaaaggag
      361 attaactatt agaggttaag aaaaaagata agagtatagt aaaaaaactc cacaaggctg
      421 taggtaaaat taacaagtaa atagtgtaat tgttataccc ggagaaagag gagaatatta
      481 tctaatcatt aattaaaccg ggtaggatta gataatatga aattaagttt tgaaaaacga
      541 aattaaatta tttgaaggca aacaaattag aacacattgg gatgaagaaa aagaagaata
      601 gtattttagt gttgtagata tcattggcgt cttaacagag agcaaagacc ccggagcata
      661 ctggagaaag ctaaaacagc gattaaaaga agacggaagt gaaaccgtga cattttgtca
      721 cgctttgaaa ttgaaagcat cagatgggaa aatgcgtaac actgatatcg ctgatatacg
      781 aggaattttt cgcatcatac aatctgttcc atcgcctaaa gccgagcctt tcaagatgtg
      841 gttaacggaa gtaggaaaag agcgggttga tgaaataatg gatccagagc taactattga
      901 tcgtgcttta cgcacatatt tagcaaaagg ttacacccgt gaatggatta atcaaagatt
      961 acaagcaata tcggtacgta aagagttaac agatgcgtgg caagatcatg gtgttaaagc
     1021 tggttcagaa tacgcaatat taagaaatga aattacaaaa gcctggagtg gaatgacaac
     1081 taggcaatat aaaaatctaa aaggtttaac aaaagaaagt ttacgagata atatgtcaac
     1141 aacagaaatt gtgttaaaca tgcttgcaga aacggccact aaagaaatta ttaatgtaaa
     1201 gcatcctgat ggacttgaag aaaatatcga gatagcaaat gagggtggga gtgttgccaa
     1261 agcggcgcgc gaagcaattg agtcaaaaac tggaaaaaaa gttataacga gtaaaaaagc
     1321 acttgattta ggaagagtaa ttaccgctat aaccgaaaaa aatgatgatg ataatgaaaa
     1381 ataataagat aatgcgttgt aattgacatg tacgcttttt aagaatatct aagatttttc
     1441 aatgtctcat taaaaaaaca tttataatat tgttttgaat ttataatatt aatattataa
     1501 tcatagttgt atctggaaat aaataccgat aggagacgaa tatgtgccaa aaaacatttg
     1561 aagaaaaact tgcggaactt gatgcttgta ttgaaaagca tcaaaagagt ccagggccac
     1621 tgatgcccac attacatgat gctcaagaaa tatttggagc aattccctta gaaattcaaa
     1681 agcatattag taagaaatta ggcgtgagtg tcgcccacat taatggtgtt gtgacatttt
     1741 actcacgttt ttctttaacc ccgaaaggaa aatacattat ttcagtttgt atggggacag
     1801 cgtgttatgt taaaggcgcg caagctatac ttgacgctgt gcaaagtgaa ttaaaactta
     1861 aaccaggcga aactagtgaa gacggtttat ttacgcttga agcaacgcgc tgtattggtg
     1921 catgtgcgct tgcccctgtt tattcaatta atgatgaaat ttatggcggt gggacaagta
     1981 agaaaattct tgaagactta cgaaaaataa ttaaagcaga ggaagaaaaa aatgatcaaa
     2041 acaaagcgtg atttagaagt aattaagaaa agaatgcaac cagattttca tttacggaaa
     2101 gaagaacata aatatcggtt attgtttcgt tataatgaaa aaacagtaat tcaaaatatt
     2161 gaaaaaataa ttgatctctg tttagcggct atttatcaag ataaacttga tgcagtagca
     2221 attaaagttt atgatacacg cgttgaagat aaattgattt tagttgttaa agagaaaaat
     2281 cgtgctgttc gttacaacat taaaaatgaa gcgatggtta ctgaaatatt aaataaacat
     2341 attggtcaag gtaaagtcgt atatgaatac gttgacgtga tagataagga ggcttaatta
     2401 tgcataaaca ttatgaaatt gtcctttgtg gtggttctgg atgtgtttcc tcaaagagtc
     2461 aattaattaa acaaaaattt gaagaaatcc ttgcccgcga aaacttaagc gattttgtaa
     2521 aggtttatat gacaggatgc tttggcatgt gtgaaaaagg accagttgtt gtcgtttatc
     2581 ctgatcaaac attttacgcg aaagtaaaaa ttactgatgt tgaagaaata gtggaaagcc
     2641 atattaaaaa aggtttaatt gttgatcgta tttgtcatca tgaatttgat gaaaaattgc
     2701 aaaaaatggt gtataaaaat ttacatgaaa tgaaattcca tagtgcccaa aaacgtttag
     2761 cgctccgtaa ctgtggggta attgatcctg atgaaattga tgaatacatt gggcgtgatg
     2821 gctattttgc ccttgaaaaa gctttacgga tggagcctaa agaaattatc caattaatta
     2881 ctgattcagg cttaagaggg cgcggtgggg gcgggttccc cacggggtta aaatggcaac
     2941 ttgcggaacg gttccgtggt ggacaaagtt atgttatttg taacgctgac gaaggtgacc
     3001 caggtgcctt tatggatcgc tcaattttag aaggtgatag tcacgctgtg attgaagcaa
     3061 tgattattaa tggttacgct attggggcaa atcaaggatt tgtttatatt cgcgctgaat
     3121 atcctttagc ggttaaacgc ctacaaaatg cgattaatca agcttataaa tacggtttat
     3181 taggcaaaaa tatcatgaat agcggttttg attttgattt agaaattaga cttggcgctg
     3241 gtgcttttgt ctgtggtgaa gaaacggccc ttatttcatc tattgaaggt agacgcggga
     3301 tgccgcgcaa taaaccgcca ttccctgctg aacacgggtt atggggaaaa cctacaatta
     3361 ttaataatgt ggaaacttac gctaatattc cgcaaattat tttaaatggt ccagagtggt
     3421 tccaaaagat tggcactgag cgttctaaag gtacaaaagt gtttgcctta ggcggtaaaa
     3481 ttaatattac tgggctcatt gaagtaccga tgggcacaac attacgcgaa gttatttatg
     3541 atattggtgg cggaattcct aatggcaaga aatttaaggc tgtccaaact ggcggaccaa
     3601 gtggtggctg tttaactgaa gaagatttag atacaccaat tgattacgat acattaatcc
     3661 aaagaggttc aatgatgggt tccggcggta ttatcgtcat ggacgaagat aactgtatgg
     3721 ttgacgtcgc taagttttat cttgacttta ttgttgatga aagttgtggt aagtgtacac
     3781 cgtgtcgcga aggcacaaga cgtctttatg aacttttaga aaaaattact gaaggtaaag
     3841 gtacactcga aacacttgaa gaattaaaac aactttgtta cgacgttaaa gatacttcgc
     3901 tttgtggttt aggtcaaacc gcgccaaatc cgattcttag tacgtttaat aagttttatg
     3961 atgaatatgt tgaacacgtc attgataaaa aatgtcgcgc tggtgtatgt aaatcattaa
     4021 cgacacttta tattaccgat aaatgtatcg gttgtacgaa gtgtgtgcgc aattgtccag
     4081 ttaactgtat cactggcgaa ttacggaagt tacaccggat tgacacaaca aaatgtatta
     4141 aatgtggtgc gtgtattcaa aattgtccag tacacgccat cattcatgta tagaggtaat
     4201 gattatgatt aaaaaagtaa atattacaat tgataataaa aaattagaag ttgatagtaa
     4261 tatcactgtt ttacaagcgg ctactgatgc ggacattaat attccgcgcc tttgtttcct
     4321 taaaggtatc agtgaaactt catcatgccg gatttgtgtt gtcgaagacg ttggtcgtaa
     4381 tcggcttgtt aactcatgta cttataaagt atatgaagga ctaaatatct taacaacttc
     4441 accacgggtt attaaacaaa ttaaaattaa cttagaacta attgctggta accaccaatt
     4501 tgattgttgg gcatgttcac gggaacacaa ctgtgaatta cttgaattat tacggcgctt
     4561 taatattgat aatcattaca acattaataa agaaattaaa aaacgtgaat ggattgttaa
     4621 taaaagcgaa tcaatggtgc ttgatagtag taaatgtatt ttatgtgggc gctgtattga
     4681 tgcctgtaat cattacactg gattaggtat ccttggtttt aataaccgcg gatttatgac
     4741 aagtgttggt cctgctggtg gtgtgaatat tggtgaggct ggttgtatat attgtggtaa
     4801 atgtattcaa agttgtccaa caggagcatt acgcgaaaaa gatgatgttg atcgcgcttt
     4861 tgacttactt gatgaagaag gcgcttatgt tgttgcgcaa attgctccaa gtgttagagc
     4921 aacattaggt gaagaatttg gttacccaat gggtactaat gtcgaaggta aaatttacca
     4981 cgcattagaa aaacttgggt tccatgatgt agtggatgtt aactttgccg ctgacttaac
     5041 aattatggaa gaaggcactg aattaattgg gcgtttaaaa gcattccttg acggtgataa
     5101 agatgtgaag ttaccacttt ttacaagctg ttcaccaggc tggattcgtt atattgaacg
     5161 ctatgctcct gaatacttag gacatgtttc ttcatgtaaa tcaccacaag gcatgagcgg
     5221 agcgattatt aaaaattatt atgcgcaaaa attagggata gaacgcgaaa agattaaagt
     5281 cgtgtcaatc atgccgtgta ttgctaaaaa atacgaagca agtcgtccag aaatggaagt
     5341 tgatggtgtt cgtgatattg atttagtact cacaactcgt gaattagcgc gttttattaa
     5401 acgccatgtc attgattttg atatgttaac aaataaacaa ttaacaagcc cgcttgcctc
     5461 ttacactggc gctggtgtta tttttggcgt aacaggtggc gttatggaag cagcattacg
     5521 tactgttaaa gcattacttg aaaaagaaga tgcttattta atcgatattc gtgaagtacg
     5581 cggctttgaa agtattaaag aagcaacctt aacaattgat ggtaaagaac ttaactttgc
     5641 ggttgtgcac ggcgctaaac attttaaaga agtgcttgaa ctagtaaaaa gtggtaaaaa
     5701 gcaatatgcc tttatcgaat ttatggggtg ccatggcggc tgtatctttg gtggaggtca
     5761 accgattact aaagctaaag actttgaaaa gattgatgta ttgaaagagc gcgctaaagc
     5821 actctaccgc atcgaccaaa gtggaaaaat ccgccgttca catgaaaatc cacatgtctt
     5881 aaaattatat gaagaatttt taggccatcc tggctcacat ttagcgcata aactattaca
     5941 tacaacatat agtaaaaagg atgtgtatta aatgaaaaag aaaattgcga ctttgttatt
     6001 aactagctta cttttaggtt gtcaaaaaca gactgttaaa ttatctttat tagtgcctgc
     6061 tggcagcccg ttattaacag ttgcaggtgt aacaagtgaa ttagaaaaag aagtgacagt
     6121 tggatccgac ttaattccgg cagcgtttac taaaggtgag aaagatttaa tcgctgcacc
     6181 gattattatt ggcgcaaagt tattcaacgc tggtaaatcg aactatcaac tcgccgcaat
     6241 gctcggctgg ggtaacttac atattttaag tcggacacta attaatacta ttgatgactt
     6301 aagcgggaaa agtggtttag cttttgctga acattcaaca ccaggcatta tgtttaaatt
     6361 agcgacaagt gatgttagtg atattaatgt aaattatgtt aaagcagtta gtgatgtcgg
     6421 cggaccattt atccaaaaac aatatgatta tgttttaatt agtgaaccag ttttaagtaa
     6481 gattagaagt aatataaatg atgaaacata tacattttcc ctgcaaaacg ttgctaaatt
     6541 acctaaaata gcgcagtttg gattatttag taatcccgct actaaagcaa aagatgtaaa
     6601 taaattttta gttaaactta aaaacaatgt tgaacaactt aacactgatc cagaaagtta
     6661 tgttgatagt attattgaca gtgatgacta ctttaaggga ctcggtcgag atgttttaat
     6721 tgatgctatt ccccgtttag atattgaata caaaagtgcg cttgaagcaa aagcagatct
     6781 tactctcttt tttgattatt taattagtgt tgacactaat ttaataggag cgacgagtct
     6841 tgatgaaagc ttcttccgtt cctaagaaaa aaccaacagc gcaaattatt gctagctcat
     6901 taggcttttt gacaatcatt ttactatgga ttattattag tgcgctgcac caaaatgata
     6961 ttatattccc cggaattatt agtactttaa aagtgttttt ttctttattc caaagagggt
     7021 ctacatattt tattttacta aacactattg ggggattaat tctcgtttta gttttatcat
     7081 ttttaacggc attaatttta agtttaatat cgttaaaatt tccaactttt aaaagttata
     7141 ttacaccctt tttagcacta tttaaaatta tccctgttcc agcgctttta attgtgtttt
     7201 tagttaattt taaacaaagc tttattccgt ttttactaac atatattgtg cttttaccac
     7261 ttgtttatga tggattatat ggggctttta ttagcattaa taaagatttg attgatgaat
     7321 taaaaattac ttcaaaactt aatttattaa tgatttttaa aatatacatt ccgattgtaa
     7381 gcaaacaagt tatttcgact ttattacaag ccttagggct aggacttaaa gtaaaagtta
     7441 tgagcgaatt tatcgctgaa gcgcctaata cgattggtta cgaactctct tatggacggg
     7501 caactttatc gatggatatt gtatttgcgt ggtcacttat tttaatcgtt atcgtcgtta
     7561 ttttagatgc tgttttagtg aattatttag gtaaacaaga agcttaaatt ttttaataga
     7621 tattaaaaaa acactgtttt tcacataatt ttttcgcaat aatatataat aagacaagta
     7681 gagggtttta ttatggaagc atggcaaatc attttaatcg ttattggagc ttatttttta
     7741 gttctttttc tttattcact attaatttat attttcacta gtgttcgctc gaagatgcga
     7801 tgtgataaaa acccgctatt tacttattac acttatgaag attatcaggg acttaaagcg
     7861 gaaacattta attttaaaaa cttcaaaggc gataacttaa tggccagaat gtatacacgg
     7921 gacggattta aagacgatac ttatcttgta ttttttcacg ggattggggc agggcatgaa
     7981 gcttatacaa cactgattaa tgatttagtg accacattag aaatgccact tttaacattt
     8041 gattacactg gttgtgattt tagcgaaggt aaaagcattg ttagtgtgta tcaaccgctt
     8101 gttgacgctc atgctttttt agcgcactta caaacaatgc cgcaatataa aggtaaaaaa
     8161 cttgtcttaa ttggtcactc gtggggtggg tttgttgcgg caaatcttta tccatttaat
     8221 aaagacaaaa atattattaa agttgttaac cttaatggtg tcacagattt tgggttatac
     8281 atcaagcatt cagcaaaagc tccatttatc tttgtgccac tttttaagtt atttaatgct
     8341 tttagatatg gcaagtttgc gtttgccgcg acacggaaca gtattaaaaa tacacaaatc
     8401 ccgcatttaa ttgtgcatgg tgaaaaagat tccgttgttg attttaatct ctttatcggt
     8461 aacttagtgt tacaagaaga taaacataaa aacatcaaat tccactttga aagcgataaa
     8521 aatcactctg cgtacttaac gactgatggc gaaaaagcac ttggtgaggt gatgggatat
     8581 tttcttacat atagtaagca aaaaaataaa gacgagacat taaaacaaaa gattgttgat
     8641 tttgattttt cacgcgcagt ggaaaatgat ccaaaaacac ttgaagttat tagacgcttt
     8701 gtaaaggggg actaattatg gttaatgaag aattaaaaac cttgttacac accttatcac
     8761 ttacgacaaa attaaaactt ttaacgggcg ctaatttttg ggaaagtgaa aaagttctgg
     8821 gcactgattt accaataatt gtaatgaatg acggaccttt tggtgtacga aaaccaacag
     8881 ttaatgttga ttctggaatg ctcatggaag taattcctgc aacggctttt ccaacaacga
     8941 gtgcactcgc tagttctttt gatcctgaac tattagagaa aatggggaag acacttgcta
     9001 ctgaatgtat tgatcaaggt gttgaccttt tgcttggtcc tggtattaac attaaaagaa
     9061 gtccgctctg tggacgaaac tttgaatatt ttagtgaaga cccatattta actagcgaaa
     9121 tggctagcgg tctagttcgt gggctacaaa gtaagggagt tggggctaca ctaaaacatt
     9181 atgctgttaa taatcaagaa aaagaacgct atacgactaa cgccattgtt gataaaagag
     9241 cattaaatga aatatattta agaccgttcc acaatgttat taaaaacact gaaccttttt
     9301 cagttatgtg ttcatataac caaatcaatg gtcgacatgc gagtcgccat tataaattac
     9361 tcacagaaaa gttacgcaac gaagcaaact ttaaaggtct tgtcgttagt gactggggtg
     9421 cagtagtgga tcatgttgat agtattaacg ctggtcttaa cttagaaatg cctggcggtt
     9481 atcaaaatac cgctgaacta gaaaaggcgg ttaaagacgg cgaaattaaa ttagaaacaa
     9541 ttgatgaaat gattaccccg cttttaaaat ttatgatgta taagcgtgat aatcctaaac
     9601 caaaagtcaa atgcgattat aagaaaaacc ttgaagtagc gcaagaaatt gccgaaaaga
     9661 gtgctgtttt gctgcgaaat gaagataatt tgttaccaat taataaaacg gcaaaagtag
     9721 gattaattgg gaattttgca attaagccgc gttatcaagg cagtggttca agtcaaataa
     9781 atccgcatac tttagtaagt gttcatgatg cttttataca aaatgatgtt aattttgtgt
     9841 acgctgatgg ttatgcagag catgaagtaa aaccaaataa aaagaaaatt gccgaagcag
     9901 ttaaggttgc gaaaaatagt gatgttgttg ttttatgtgt tggtttgcct gaaagttatg
     9961 aagtggaagg ctatgaccgt aaaacattag acatgccacc gagccataat gaattaataa
    10021 aagaagttgc aaaagttaat gaaaatattg tcattgtctt atcaactggt tcgccagtta
    10081 caatgccgtg gatcaatgat gtaaaaagta tcttacttac ccacttagcg ggtagtatga
    10141 cgggaccagc gacatataat ttactgtttg gtgttactaa cccctctggt aagttaagtg
    10201 aaacttatcc gttaagttta gataaaggat tacctggtgg agagtttctc actagtgatc
    10261 aaaatgtttt atatcgtgaa tcactttatg ttggctatcg ttattacgat acttttagag
    10321 aacaggtact tttcccgttt gggcacgggt taagttatac gcaatttaac actagccatc
    10381 atgaaattga agaagtaaaa gacggttata acatcacgat ggaagtgacg aatatcggtc
    10441 atatccgcgg cgcagaagtt attggtattt acacaggaaa gagccgtagt ttgctctacg
    10501 gtcctcgtaa agaattacgc gcttttaaga aggtgtatct tgatccaggt gaaaaacaaa
    10561 agatcaatat attcttacct actgagtcat taaaagctta ttgtccagta aagcaagagt
    10621 gggtgcttga aaatgtaaat tacaatgttt atgtcggaat aaatgtacaa accactgatg
    10681 agatcttcca aatcttagtg gatgacgcta atgatttaag ctatttaaaa aattatcaaa
    10741 aagaaaatga aatgtattat cgtcgcgccg attttaatcc atcaattaaa gattttacaa
    10801 cattatttgg tggtgaatct ttaccattat ttttcgctaa caggaagcgt cccttcacac
    10861 ttgctaataa cattagtgat acgactgatt actggtgggg acgaacaatt tataagatta
    10921 tggccaagac ttcacgcaaa ctagcgggtg atgatgtagt actccaagag atggctgttg
    10981 aaacgattaa agaattaccg tttagaagtc tttcagcctt aagttctgga gaagtaagca
    11041 ctaatcaacg agacgctatt ttactattag taaatggcaa accgattcgt ggtattttca
    11101 agtttttacg aaaaaagaaa tagaaacatg tatgaatata cctttcaagt tgtcttaact
    11161 aaggacttaa gtcctttcat gctataattt acctatgagc gaagagaaag ttagcacaac
    11221 ccaggattta aaagtacaaa agcaaacgaa gcgtgatcgt cgctatctta ttaatatcat
    11281 cgtaatttta ttaattactt ttgggtacac tacttactca ttatggaatg aactcccagc
    11341 ggtaataaat gtgttccgtg gcacggaagc tgattaccgc atgattataa taattattgt
    11401 tttaattgct ctaaagtttt ttattgaagg cattatctta tttatttttg ctaggcttta
    11461 taccccgact tataaaatac atcgcggtgt cgcgaatgct tttattggtc aattttataa
    11521 tgatattact ccgagtgcta caggtggtca atttgcgcag gtaaaaactt ttgcgcaaca
    11581 aggagtgcca gtatcaattg gagcttccat tatggtgatg cactttattt tgtatcaaat
    11641 tgtgcttgtt gtttttggtg gattacttat tcttttaaac cttggagaat ttgcgcgact
    11701 tggttcggtt agtattttta aacttaatat tccagtgtgg gtttttgctt catttgggtt
    11761 tttagctaac ttagttacga tagttgggct atttttacta gcgtactcga gacgtgttca
    11821 tcactttgtt attaatgttg tagtcgatct tttagcgaaa attaaaattg ttaaaagacc
    11881 tgaatatcgt aaaaataaac tttatacttc aactgagaac ttcagaattg agttacggcg
    11941 cttaagttca aacgtgccag cttcaattct tattatggtt ctctttttct taaaatttat
    12001 tgttaactat tcagttacat attttgtggc tttaatgtta aatccagcat tgtatggcgg
    12061 tctttccttt tgggaaacaa tttctaagac tggtttctta ggaatgatta caggctttat
    12121 tccaattcct ggcgctgctg ggtttgccga atatttttac gaaatcatct tctcccctgt
    12181 ttttggtgga gatgccgcct ttacgaaagc agttcagctt atctggcggg tggcaacatt
    12241 ttatgttaac ttaatcgttg gcggatttgt gacggccttc tatcgttcga gtatggaaga
    12301 aattgttgat gaaacgggtc acgttaaaac atttgaagaa gttaagagtt acacctttgc
    12361 tgaacgtcgt gaaactagtg tgacgatgta tgccacaagt caattatcaa ttaaagaaat
    12421 tcagcgtcgt ttatcaccta agaaaaaacg aagatggcgc aatcataaag aggatcaaga
    12481 ataatgcgaa ttgcaatgtt tactgataca tttgtccctg acactaatgg ggtcgccgtt
    12541 tctagttata atctttttaa agcacttaac gatcgcggtc attactgtgt tgttgttact
    12601 tctaatcctt tttccaaaga agtaacattt atcgataatg ttttgcggat tcccggaatt
    12661 gaaatgaaaa aactctatgg ttacaacttt gcgggaccaa accgtaaagc acataagatt
    12721 ttaaaaaagc ttaaaatcga tatcgttcat gttcaaaatg aagcggggat gggtatattt
    12781 ggaaaaagtt ttgctcgtca acaaaagatt ccttatgtgt atacatatca cacaatgtat
    12841 gaagattata cttattacgc gacaaaagga cggatggaaa catttgctaa agctattatc
    12901 cgtcgctttt cacgatggca agcagaaatt gctgatgaat ttattgtacc aagtgataaa
    12961 acaaaggtgt cacttcgcga atatggggtt cagcgttata taaatgttat tccgtcgggt
    13021 gtcgattttt caaaatttat gtataatcct gaagataaac ctttccttga tgaatataaa
    13081 aaggaacacg ggttagacgg cacttttaac ttagtgtatg ttggacgtgt tgcaaaagaa
    13141 aaaagtattg atgtcgtaat taggggttat gcactttatg ttaaaaagaa tctaaatcat
    13201 aatactagac tcatcatcgt tggcggagga ccggcaattc ctgaattaga agaacttgca
    13261 gaaaaattac aaattgctcc gtttgtccat tttgtcggca aagtcttacc tgcggatatt
    13321 ggcaagtatt atcatttagg ggaagtgttt gttagtgcct caattagtga aacccaaggc
    13381 ttaaccttta tggaagcgat ggctgcagaa cgccttttac tttgtcgttt tgatgaaggg
    13441 cttgtcgggg tgattagaga cggtgaatca ggtttctttt tccgtagtga agaagacttt
    13501 ggtgagatgg tgaagaaaat tcgcagttta tccacaaaag aaattcgcaa tatattagat
    13561 gaagcaaaca atatagttga ttattacagt aatgaccgtt tcgtagaaag atgcgagcac
    13621 gtctatttaa gggcccttcg taatagttgg taatggttaa aattataaat atcacattga
    13681 agaaagagca atacaatatc acttttgata atggtgaaga actattagta agtgcagaaa
    13741 caattgcgga gttttttctt tataatggca aagagttatc actaaaagac ttagcgaaaa
    13801 ttaaagaata tgaagcacag actaagcatt ttagttacgc tagtaactta ttagcgagaa
    13861 gaccttacac aagtcatgaa atacgtgaaa aactccgcaa aaggcgtgta aatgaggaag
    13921 ttattgattt aatagtcgat aaacttaagc gtcttaattt aattaatgac ttacggtatg
    13981 cacttgataa aatacagtac ttaattaatg ttaaaaagtg ctctactcgt gctattgagc
    14041 aagatttatt aaggcgtggt attcacccct atgttattaa ggatgctctt gcgcaatcgc
    14101 cacgtgacga gcgacgtgaa cttgaaatac ttatacctaa attactaaaa tcgtatcgta
    14161 atgaatcact gcgccgtgct tatcaaaaac ttaaaactaa attatatagt ctcggctttg
    14221 aaagtgataa tattgatgaa gttttagcga aatttaatgt agaagattat atcgatgaaa
    14281 cagaaaatat taagaaagca ctagtgaaac tagaaaagaa ggcaaaaggt gacactaaag
    14341 tgttgtataa taaattagtt atcgcgggtt atcccgcttc cttaattcgt aaattgttgg
    14401 aggattatag ttatgaaaat taaagattta aatgatggca ttcgtgttat tgaaaattat
    14461 ttagtgacga atgtaactaa aggtgtatca aataacggtc aaatgtatct aacggtcagt
    14521 ttgcaagata atacaggcca aattgaagcg cgggtatggg acgcaaatga aaatgatgta
    14581 gaagtattta aacaaggtaa ctttgtgcgg gttgtcgctg atgttattga ataccgttcg
    14641 aatttacaac tgaaagtcat gacgggtgaa gtcctaaatc ctaaagaggt tagacttgat
    14701 gaatttacaa tggcaagccc ggttaaactt gaaactttaa ctgcggaact taatcgttta
    14761 ctctcttcat ttgcggacac ggatattcag cggctcgtgg aacgactaat cagtgatcgt
    14821 tatgaagctt ttataactta tcctgccgcg agcagattac atcatgaata tacacacggt
    14881 ttgcttgaac acacaattac catcgctaat cttgcagatc gtatcattga ttattattta
    14941 gagttgtatg aaggtgattg gcatattaat cgcgatttag ttattgcggg cattttactg
    15001 catgacttag gtaaaacaat tgaattaagt ggaccagtgt taactagtta tacagttgct
    15061 ggtagacttg ttggccatat ttcactaatg aacgctgaac ttttaaaagt tgcagaagaa
    15121 cttgaaatta aaagtgaaat gccaattctt ttatcacata tcatcttatc gcatcacggt
    15181 aagcttgaat acggttcacc tgtaactcca atgacaaaag aagcacaact cgtgagtatg
    15241 attgatgact tagatgccaa aatgaaatta attgaaaaag cgctcaacac aacaaacagc
    15301 ggtgaattca ctgatcggtt gtgggcgctt gattcaacga gtttttataa accgaaaaaa
    15361 taattataaa ccagacttaa ttttttccac gactgctggg aagtttaaat cgccatcaag
    15421 gtgttcaacc atggcgattt ttaatccgtc ttttttacca taacgcggga ttacatggac
    15481 atgaaagtgc gggacgcttt gaccagcggg cggataatta ttaattaaaa tattaacgcc
    15541 ttgcgcttta agtgttttaa tttgcacttg gccgatacgt tgcgcgacat ccatgacgtt
    15601 gtacattaat ttacgcggag tgcctaaaaa tgtttcgtgg tgttctttag gaataactaa
    15661 agtgtgaccc ggagcggttg gggaaatatc aagaaaagca aggacatcat tatcttcata
    15721 gacgatggca cctgggattt ctcgacgaat tatcttacaa aaaacacaat ctttactcat
    15781 tagtgttcct cctttatcta tatattaatt atagcaaata tatgtaaata aaaaccgcag
    15841 gggttaacct gcggtcgtta tttaataaat agaaggtatg cttatttctt ctttttttca
    15901 tcatcgaaga tatcagggaa tttttctttg aagaaatcat agacatcttc gtcatggaag
    15961 acaatcttta agtctttcat gtagaagtta agcgcattag ttttagtcgc atcgagtttc
    16021 gctaaagctt cagtaacatg ggcaacaatt tccgcaattt ctgcttcagt tttgaaatgc
    16081 tcatatgaat ttaagtcatc atctttagcg gcaagtttag cggtgttaac ggcttcaacg
    16141 attttaatga tgtagaatgt atcgttttcg aagaagagga agttacgatt gttacctttt
    16201 tcataagttt tggggactag gtaataatcg tcatgaatat tacgaacgaa ggttgacgca
    16261 actttcttgt tttcggcgtc gccttcccat ttaccagcat cagcaaggac accagcgtcg
    16321 tcttttaaga agtcaacact attagcgaca ccaatgttga ataagcggga gcggatagct
    16381 tctggtagac tactaaagcc accatcttta ataccccagt ctttcgtgac gaatgattgt
    16441 ttacgcactt cattaatttc aatttgtttc ccaacttctt ttggatattt accactgcca
    16501 gtgaaacgat cttcaatcgc tttgtcagtt aagtcagggt tagctttaat tttggcgaaa
    16561 tcactttcaa tgtcaccaat taaggttttg ttaacttcag cggttttaag tccagttgcg
    16621 gctagaagtg cagcttcatt accaataaag tctggttcat aacctctcca agcattagca
    16681 aggatattga agtcagcagg ctcggcgcct aagatattat tatcaataaa ggcattaacg
    16741 agtgcactag cggcggttgg gtggttacta ttatttttaa tagcgacata ttcaaccttc
    16801 cgggcatagt tacggccgag ggtggaagtt tgttcttcct taacgaattt ttctgttaat
    16861 ttttcgcgat aaatcttatc aacatagcga tcttgcatgt aatgcttata gtaatcgata
    16921 tggacaagtt taaaggctgg atcgtcaaag ttatctttgt tgacgattgg aaggaataag
    16981 ccttctttgt agaatggtaa ggtatcaatc gcaccaatgg cgacttcgtt gccgttagcg
    17041 tccactaggt agctattttc cgttccgtat actctttcaa cgacgtattt cttttcgtca
    17101 aagattgagc gatatgtgta tgaaccgctt aatagttcat catagaaata atgtttcgcg
    17161 cgtgtatcga ctgcgagttt gaatgggtcg tctgcggcta atttgtcgta tggaccaaat
    17221 tcatgttcgg caatcgtaag taacacttcg tctaatacga attgtttaaa gttcgcggag
    17281 tcatgatatt tcttatagac attttctaat gtgttgttaa caacttcgct taattcgtcg
    17341 acaagcacag ttttatctaa atcaactggt tcggcggtaa ttttgtcaca accagttaag
    17401 agaagtgcag cgacggctaa gcctaataaa tatttacgtt tcactctatt aacctccgta
    17461 attgtagtca ttaccagcct tgaaatcatc aatatatgtt tcaggaacaa ggcggtcggt
    17521 acgtgcgact tcctgttgat ctaaatattc aatccattcg ctccataatt tatcaagatc
    17581 ttggtcgagt tggaattggg aacgagcgta ttggttcgct agatatttat caagcgctgc
    17641 tttgagttca gcatcatgga acttcacatc aagatatgtc actaactttt ggaaaatgcg
    17701 atcgttgatt gttgggtcaa atgctttaac ttcattacga atcttgtcga ttctttcttt
    17761 tgtgccacgg gcgtcaagtt ctaagaagtt gacaaaggca tcaatatcgt taccagcatc
    17821 gtcttttggg aaagcagcgg ttcctggggt ttcgattgtg tagtagtctt ttaaagtaac
    17881 gccgtttacg gtgtcataaa gagcactgcg ttcgacaacg atgaagtgga ttccttcata
    17941 acttccggct gaaccagcgc gcacgactaa gataacatta cctttttcgt cggttaaaac
    18001 ttttttacca ccgagttctg gaagagcttt aaatccagga agggcagcaa attcagcttt
    18061 ttctacaccg acttcgtcaa ggacattaac ttcatttgga gtaatgactc caatgttatg
    18121 tttattaaag tatttattaa agataacgtt acgtgggaag aaggcggcat caccgtcatt
    18181 tacttcttta ccagcatcgc tcttttcgat ttcacgaaca gcggcgagtt taagcgcagc
    18241 gccgtaagga atttcgtcaa gtccgagtgc gtctaattta gttttggcgg tgccaggaat
    18301 tttaagttta gccgcttttg cttctggagt cataactccg tcagcgttgt aaagagcgtc
    18361 ataagcataa actcctaatt tgaactcatt cacaaagttt gtggtgaagt tcataatgcc
    18421 taagtcacca aattgacggg cactatcttc gtcttcacta tagcggtttg ctaaaagtcc
    18481 gaaggtgtct tgatgatttg ctaaggctac gataacagaa cttaatttgt tcgcatcgtc
    18541 tttggaaatc ttaccggtaa caaagtcgtt gtttgcggca ccgactttaa cgagtaagtg
    18601 acgaacgtga tatggaagtt tgttgtttaa gtatccttcg taaacaggat tgttgttttc
    18661 atcaagaatt aaatctcctg cttgatttcg ttcaccaacg attaattctt ccattgcgtt
    18721 tgtgatccag gcgccttcgc ctccatcaaa gaattgtttt ttgaggcgga cttgcatttc
    18781 atcgtatgcg agtttctctt ctaattcttt taatgtttcg acgtttttag tcttaagaat
    18841 tttcttaagt tcatcgtcgt acttgccacc acctttggcg ttattctttg cttgttcttt
    18901 cacatcgtta acgctaaggg cagcaatatc atagatttcg gttcgctctg tagtttgctc
    18961 ttctttatta aagagagcgc gaaccacaac ttcatagacg gcgtcgtaat aatcctttag
    19021 tccatcagag gattttaaat agtcttcaaa catatcccct gcattaattt gtactgggtt
    19081 accatcgatt gtcaccgtca caatattgcc ttttttgtct gcgactaaat ctttactaca
    19141 tccggtaaga agtagtgccg atagcgctaa accagcaata atgcgttttg gttttctcat
    19201 ataatgctat acctcctaat aatatgcttt tatattctat acttaacttc caaccttttc
    19261 aacaaaaata agcgttaata gtcgataaat taaccaataa cttattttta tataaaaata
    19321 gaatacgaaa atattgaatt tttcatgata ttttaaggtt tatacaaata ttatgaaaaa
    19381 gcaaaagaga tatttgtgta ttttctaaag tttgaatttt caaaaatgat aattaataaa
    19441 tcaaaattaa taggaacgaa gatattattt tattttgtgc ttatataaat tgagacgcag
    19501 tgcattaaaa acgacgagaa gactagaaat aatatggagg aaaccaccta ctagtggtgt
    19561 agcgatatta aatattgcaa gcggaatcat tactaagtta tataaaaatg cccatattat
    19621 attttgttta atattggctg ttgttttctt tgataaatca atagcaatta acactagttt
    19681 taaatcgtta tctaagagag ttacatctgc tgtatcagtg gcgatatcag aagcattact
    19741 gacagagaaa ctaacatccg cttgttttag ggcaggtgca tcattaattc cgtcaccaac
    19801 aaaagcaact acactatgct tttcttgtaa ttttttgatg atatgagctt tttcattcgg
    19861 taacacacca ctataataat tctcaattcc aactttattt gctaagtatt tagcggtatt
    19921 ttcattatcg ccggtaatta ggtagacatt gatgttcatc tttttaagtg ctgtaattaa
    19981 agtctgactt tcagctttta tttcatcttc caaaataatc atatttacga cctttttatt
    20041 aatcgcagta aagatgacga ccttaccttc acctgtaaat ttttcaaatt catttaagaa
    20101 aggattacta atattagctt cagcaataaa agtagcagaa cccactataa tttgatcttg
    20161 taaatatgtg cctttaatac ctttgccatc aattatttca tattcctcaa ttgcaaaagg
    20221 aataatatct tttcgatagt tattgataga ttttccgatt ggatgagtgg aatggatttc
    20281 taagcctttt gtataactta aatatctaat atcgccaaga taatcgctga ctattaaatc
    20341 acctttagta agggttcctg ttttatcaaa agcaatcgca tcaattttat tggcgagttc
    20401 aaaaaactca ccgcctttat atagaatttt atttttaaat gctaatccgc ttgcaactgc
    20461 aattgaagtt ggtgttgcta acccaagagc acaaggacat gaaactacca agaccgcagt
    20521 agtcacctcg agagaaattg agagttcttt ccctaaaata aattgataaa caagaaatga
    20581 tacaagggca atcattaaaa taataaaaac gaaatattta gcaataacat ccacagcttt
    20641 ttggaatttg ggcttaataa gcgaagtttc ttcaaccgtt ttaataattt tagctagtac
    20701 agtatcactg ccgatagcac ttactttaat caaaattgta ttagataaat tagtggtaga
    20761 ccctacagcg gtatcaccta cactaagata tttagggatt gattcgcctg ttataactga
    20821 atcatcaata taactagcgc catcaataat ttcaccatct aaagctattt tttcgccggc
    20881 aagcactttt ataacatcgc caacattcac atcattaagt gaaactattt gttcctctcc
    20941 gttttttata agcctaactt catcaaccgc aagtgccatt aatgatttta atgtatccgt
    21001 tgtttttgct tgtgagagat gttctaaata atcaccaatt gttaccatcc aaataagcat
    21061 accgctcgtt tcaaaaaaca attgagtttt atctgtaaaa atagttaaat acaaactata
    21121 aaagaacgct actgaagtac ctaaaacgat taaaacatcc attcctaaat ttttattttt
    21181 tatttgataa aaagcatttt tataaaatct aaatccaaaa ataaattgca atattgcagc
    21241 aaaacccatt tggaaatagc cgtttagcat aatatcgggg acaatgttta atccaaaatt
    21301 atcaaacatt gtgtatgcaa gtggtaacac taaaattgtc gcaactatta aacggacttt
    21361 ttcatatttc cgacgtcttt tttcatcgta atcaattggc gcagcaggga acccaatcgc
    21421 ttttaactct tttaatatga tggtgaaaga atctttatca ctatatgtaa aggtcacttt
    21481 tttctgattg acatttactt ttgcttcgat gtttaatgtt ttaaagtgct cagtaattgc
    21541 catggcacaa ttcggacaac caatatttgg gattattaat gttgttttca tatttcctta
    21601 ccagttagcc gttgctaaca ctataattat aatagtataa taaaactaat tcaagtaata
    21661 ctaattgttg ttaggatagt ttacttgaat gaaataaata atgatgaacg gaggtgaaac
    21721 ataaacattg caataaatga taaaactaag gatgttaggg aatataaaat ccttttttac
    21781 tgaaataaaa attaataaat aagcatataa aacaatgata tgttattttt aaataaaaat
    21841 aggatagtaa aacagtttat tttttaggtg ttttaaggta tcttttatat gcatacaacg
    21901 gaaagagagt ttgtaaagat ggtattaatc gaaattaaag atctacatgc atccgtaaat
    21961 gatattgaaa tattacgtgg tgttaactta aaaattaacc gtggcgaagt agtggcctta
    22021 cttggtccta atggtcatgg caaatcaacc ttactacaag taattatggg tcaccctaat
    22081 tataaagtta cgaaaggtga aattttatat aaaggtcaag atgttttagc aatgagtgtt
    22141 gacgaaagag ctagagcagg gctttttctt gcaatgcaaa acccaagtga ggttcctggt
    22201 gtacagatta gtgatttctt acgcgccgct atcaatacaa aacgtgaaaa accaattaaa
    22261 ccaattgaac ttttccgcgt gattgataaa gcaagcaaag aaatgcaaat gccccacgac
    22321 cttgcctcgc gtaatttaaa tgaaggtttt tctggcggag agaagaagcg tcatgaaatt
    22381 ttgcaaatga aattattaaa acctgaattt gcaatgttag acgaaattga ttcgggtctt
    22441 gatgttgacg ggattcgaat tgtctcgcaa caaataaatg aagagaaaaa agataaaaca
    22501 tttctcgtta ttagtcacta cgcaagacta tatgaactta ttaatccaac gcgcgccatt
    22561 gtaatgatta atggtcgagt agcgattgaa ggcggtatgg atgtcattaa gcgcattgac
    22621 caagaaggtt atgaatggat tcaagtagaa cacaacatta atattgaacg cgaagacaaa
    22681 cgcccaattg tattagaaat atgtgcggtc aatgaaagaa gtaaacaatg agtgttttta
    22741 aagcgttatt aataaataaa atgaatatta atgatgacac gctcatctta agtgaaaacg
    22801 atactgttta tcatattttt gacgaaaacg acttacctac ttctgtgact aaagttatat
    22861 taaaatcatc aaaagacatt gattttgtcg ttgtattacg gcaaaacaaa aagttcatta
    22921 cctatgaact tgcgccatat acgcgcggaa aagtaatttt tatgtgtagt gaggaaaatc
    22981 ttagcattga tcgcgaaatt attttacttg aaggtgctga agttaaatta attatgcctg
    23041 atttccataa tggtaatcgc aaggtaaata ttgaaacgaa actatcagaa agaaaagcta
    23101 gagcagaatg gcatcttgct acttattcac aaaatattga taagaaagta tttaatatct
    23161 cgttttcaca ttttggaaat gagtcatttg ctgatatgca taactacggt gtagtactta
    23221 atgcatcaac tcttatattt acaggtgaat caacaatttt tgaaaatgtg aagggcgcgg
    23281 aaacccatca aaccgcacgc attattgttt ttgacgaaaa tagccatgca gaggctaatc
    23341 caattttgaa tatttatcat aatgatgtcg tcgctgcaag tcacgccgct actgtcggac
    23401 aagtaaatgc tgaacatctt tattacttaa agtcccgcgg acttacagaa aaagaagcta
    23461 aacttttaat tacaaaaggt tacctgatgc cgatattaaa ttatattgaa gataaagttt
    23521 taaaagagaa ctgcattact atgcttgaga gggtattata atgtacgacg tttataaaat
    23581 tagaaaagat tttccggttt ttgataataa caaaacaata tatttagata acgctgctac
    23641 aacatataaa ccacaagttg taatcgatgc agtgactaat tatttatcaa aagaaaccgc
    23701 taatagtggg cgcggcgatt atcaaaatgc ttatttagtc gataaaagag ttgatgaaac
    23761 gcgcgcttta attcataaat ttattaatag taaacatgtg caagaagttg tttttaccca
    23821 agggacaacg gcctcattaa atatggttgc taagtgttat ggtgagaagt tcttaaaaga
    23881 gggtgacgaa atattattat caattgctga acacgcatct aacaccttac cctggatgca
    23941 agtggccaaa aaaactggtg ctaaaatcgt ctatatgcca cttagtgaag acgggaaagt
    24001 cactccagaa attgtcgaaa aatatatgac agaacataca aaagttgtca gtgtacttca
    24061 tgttagcaat gttttaggtc atgttcatgg tattaaacaa attgcgaaaa tagtgcatga
    24121 taagggcgct attattgttt tagatggtgc gcaaagcgtc ccgcatatga aagttgatgt
    24181 tcaagactta gatgttgact ttttagcgtt tagcggacat aaaatgttag cgccaacagg
    24241 tatcggtgta ctttatggta aaaaagaatt attagaaaag atggatcctt accaaacggg
    24301 cgggggcatg aattttagtt atgataaaga agggaattat gaattattta aagtcccact
    24361 taagtttgaa gcaggcacat taaacatcga ggcgatttat ggcttacatg ccgcgattga
    24421 atatattaat aagattggta ttgaaaatat tagtaaacat gaacataagt taagaacata
    24481 tgctgtttct aaattaaaag aaatggatca tattatttta tataacgaaa atgctgaaac
    24541 tggtattatt actttcaatg ttaaaaatgt gtttgcacaa gatgttgcta catattttaa
    24601 tagtaagaat attgccgttc gtagtggaga acattgcgct aaattgctga aagaacattt
    24661 acgcgaagtg gcaactgtac gtgcaagttt atatttgtat acgacaaaag aagaaattga
    24721 tgcctttgtt gaggcagcta gaacagcgga gaattattta gatgcctatt tttaataacg
    24781 agcttatgcg ggcgacaatt ttaacgcatt atgaaaaccc ttatcacaag gaaaccccgc
    24841 aaaatgcgga tgaatttatg caaattcacc ttacttccag tggttgtata gataatttaa
    24901 cactttattt aaaagttaaa gacaataaag ttatcaccgg atattttgat ggatttggtt
    24961 gcactatttc gattgcctct acctcaatcc tccttgaaat gattgaagga atgaatgttg
    25021 atgctgtctt gaaaataatt gcggaataca aaaaaatgct caacagcgaa ccttatgaca
    25081 tagatattct taatgaagca attgtatttg caaatacagt ccggcaacca agtcgcattc
    25141 gctgtgccac aattgggtgg gacggggttg ctgaattaat tacaaaacat aaagaggagg
    25201 aaaatcacaa tgactagtgc tgatgaatta aaatttggta cttatgctta tggtttctcc
    25261 gatgaccaag aatcaattgc tagtactggt attggtatta acgaagatgt tgttcggcaa
    25321 atatctgcct ttaaagaaga accagaatgg atgcttgagt ttcgacttaa atcacttcgc
    25381 gcgtttgaat taatgaaaaa ccctaaattt ggaccatctt taaaagatct cgattttgat
    25441 tcttatactt attttaaccg tgttgtaaaa gatgaggcac gctcgtggga cgatgttccg
    25501 gacacgatta aagaaacttt tgaaaagtta ggtattcctg aagctgagcg taaatttctc
    25561 gctggtgtgg cgacgcaata tgattccgaa gttgtttacg ctagtatgat tagtgaattt
    25621 aaagaaaagg gtgtgatttt ttcatcaact gatgaggcac ttaaagaaca tcctgagata
    25681 tttaaaaaat attttaatac cgttgttaaa tataccgata ataaatatac agccttaaat
    25741 ggggctgtat ggagcggtgg ttcatttatt tatgtgccaa aaggtgtgga actagataaa
    25801 ccactccaaa gttactttag acttaatgcg gcaaagatgg gtcaatttga aagaacatta
    25861 attattgtcg atgaaggggc aagtgttcat tatgttgagg gttgtactgc tccagtttat
    25921 agtgaagact cattacacgc tgctgttgtt gaaattattg tacataaaga cgctaaatgt
    25981 cgctacacta cgattcaaaa ctggtcaaat aacattatta atctcgttac aaaaagagca
    26041 gatgtttatg aaaacggaca gatggaatgg attgatggga atattggtag ctctattaat
    26101 atgaagtatc ctgcttgtat cttacgcggt gaaggagcac gcggcacaac aatttcgatt
    26161 gctgttgcag ggaaaggtca atatcaagat gcaggtgcaa gaatgattca cttagcacca
    26221 aatacatcaa gtaccattat ttctaaatca gtcgtacata gtggcggcac ggcaaattat
    26281 cgcggtttag tgcgacatac aaaaaatgct cctaattcac aaacacatat tgaatgtgac
    26341 actttaatac tagacgggat atcaaaaagt gacacaattc ctactaatga aatacataac
    26401 aacgagtcat ttattgaaca cgaagcaacg gtaagtaaaa ttagtgaaga acagttattt
    26461 tatttaatga gtcgcggttt aagtgaagaa gacgcaacgc aaatgattat tatgggcttt
    26521 attgaaccat tctctcgtga attaccgatg gaatatgctg tggaattgaa tcgtctgatt
    26581 aaactagaca tggaaggctc acttggttaa tgaagtttga ttatgacata atcgtcgttg
    26641 gcggtggtca cgctggggtt gaagctgcgc tagctagtgc ccatatgggt aaaaacaccc
    26701 tgcttttaac aatggacatt aaaaaaattg ccaatatgcc atgtaatcct aatattggtg
    26761 gtagcgctaa agggattgtg gtgcgtgaag ttgacgcgct tggcgggatg atgggtattt
    26821 ttgctgatca cgatccacta caaattaaaa ttttaaatac tggtaagggt cctggtgtcc
    26881 gttgtttaag agcacaagaa gataaagttg gttatccacg ttatgtccaa agcattgtgc
    26941 taaatactcc aaatttaact gtaaaagaag caatggtagt ttcggtcatt catgacacag
    27001 aaaaagtgta tggggtccgc ttggaaaacg atgaagaaat attggcagag gcaacaatat
    27061 taacaactgg aacatacatg gaatcaacaa ttttagttgg tcacactgtt aaagaaattg
    27121 gtccagaagg agaccgttct agtaaaggtt tatcgccgta tttggagaaa atggggctta
    27181 aaatctttag acttaaaact ggaacaccag cgcgacttaa aaaagatacg attgattata
    27241 gtgtgatggt tgaagaacca gggatggaag gctacttaaa tttttcctat atgagtgagc
    27301 gctatcacac ccttgaagaa caggtgctct gccacttact ctacactaca cctgaaacgc
    27361 acgcaattat taatgaacat ttaggtgatt ccgcgatgta tagcgggctt gtaacaggaa
    27421 cgggtgctag atattgtcca agcattgaag ataaaattgt gcgctttagt gataaagaac
    27481 gtcatcaatt attcatcgaa ccagaatcac gttacactga ttcaatgtat ttacaaggat
    27541 taagtacttc aatgccagtt gaagttcaag aaaaaatgat tcgcagtcta aaaggtttag
    27601 aaaatgttga aatattaaaa tatggttatg cgattgaata tgatgcaatt gatccacttg
    27661 aacttaaacc aacactagaa cttaaaaagt gggataactt atattgcgct ggtcaaatta
    27721 ttggcacaag tggatacgaa gaagcagcgg gacttggtct tttagcagga attaatgctg
    27781 tgttgaaaat agataataag ccacccttaa ttttgaaaag agatgaggcc tacattggtg
    27841 ttatgattga tgacttagtg acaaaaggca ctgaagagcc atatcgttta ttatcaagta
    27901 gatctgaata ccgcttactc ttaagacatg ataacgctga ttcgcgcctt accgaaattg
    27961 ggcgacaagt tggcttagta aaagatgatc gttatgcgaa gtttttaaaa agtcaaaaag
    28021 ccatagcgga tgctcgagca attttagaaa caacacaagt aagcgcggat attcctgtcg
    28081 ttgaatttct cgaaagtgtt ggttatccta attattcggg cggattaacc attgctgaac
    28141 ttattaaaag accgcgagtt acactagatg atttattaga gcttttagga cacttaacac
    28201 ttgagggtct taaccacaat gatttatttc aactagaaac tgatataaaa tatgaaggat
    28261 atattaataa gcaacgccaa gaagcaaagc gtttaaagaa gcttgaagaa gtagaaattc
    28321 ctgcttcttt cacttatgat gatttagatg gtttgcggat cgaagcgcgg cagcgcttag
    28381 ctaaagttaa accacttaca attggtcaag ctaaacgtat tagtggtgtt aatcctaacg
    28441 atattattat attaatgaca tatttgagga ggtatggtaa aaatgaaaaa taacccattt
    28501 ttgcagtact tagacgagca ttttagcgga aatgctgaag tgttaaaagt gcaatttgca
    28561 aagtatgctg cacttatcga agagcataat aaggtacaaa atttaaccgc aattgcccca
    28621 agcgaatatg aagaaaagca ttttttagat tgcttattac ttcgcgaaac atataatttc
    28681 cgcaatgaaa cattattaga tattggtagt ggtgctgggt ttccaggaat tgttttagcg
    28741 attgcttatc ctgaattaca aataacttta cttgaaccaa ttaagaagcg ttgtaatttc
    28801 ttaagtactg tcgttaaaga attagaactt acaaatgttg aagttgttaa tgaacgtgcg
    28861 gaagattatg ccgcaagtaa acgcggtgaa tatgatttag tcacaagtcg cgctgtgtct
    28921 tcgctaagta taatgcttga actaagcgta ccgtttttac gcatcaatgg cagtgctttg
    28981 atgatgaagg ggcgaaatta taatgctgaa ttaagtgacg ctgaaaatgc aatttacacc
    29041 cttaaagttg aagttggaaa aacagattta catttcttac caagtgacgg tagtatgcgg
    29101 gtcaacgctc gctttattaa aaagggaagt actcctaaaa aatatccgcg tccattcgca
    29161 caaattaaga aaaatccact ttaaagtata tttgatatac ttattacaag gaatagtatg
    29221 gctaaaatca tcgcaattgc aaatcagaag ggcggtgttg gcaaaacaac gaccgcaatt
    29281 aatttaagtg cctcttttgc acattttggg cgcaaggtac ttttaattga tatggacccg
    29341 caaggcaata gtagccgtgg gcttgggctt gatcctgcga cgattaaaaa tacgatttat
    29401 catacttttt tcaagggcat taatattaat aaagctattt atcgcacacc actaaaaagt
    29461 ttagatatta ttggcgctaa tcttttactg gcgcggactg atgtggaaat tagcacgatt
    29521 ccgcaacacc cgtttaccgt tttacaatca aaaattaaag aaattgatcg ccgttatgat
    29581 tatatattta ttgattgccc gccttcactt ggactattaa gtactaatgc tttagtggcg
    29641 gctaatagtg taattattcc tgtgcaatgt gaatattttg cgcttgaggc tgttgcccaa
    29701 gttttagcga cgattaaaca agttcaagcc tcacataatt cggaactgaa aatagaaggt
    29761 tttctgttaa cgatgtatga cgaaagaact agactaggaa ttgaaattag ccaacaaatt
    29821 agaggtctat ttaaagaatc aacatttatg acaccaatcc cgcggaatat cgctctagcg
    29881 gaagcaagtg ctaaaatgct gccaaccatt ttatataaac cgacttcgaa aggttcatta
    29941 gcctacttaa atgtagctaa ggagattatg gagaatgaag aacgagccga agaataattt
    30001 gttaaaagaa cacctttcag atttaatttc taaatattcg caatcaaatg ttttagaaga
    30061 acttgcgaaa gcacatacga atcggccgat tcaaatgttg ccgattgatt taatagtaga
    30121 caataatttt attaaaaaga ttactttgtc ccgtaaaaaa attgcgcgct ttgttgaaaa
    30181 ttatcagcgt aatattttac tagaaccact cgtggttaga gcaaaaggtg ataagtacga
    30241 agtaattatt ggacgccggc gtctagcggc agcaagactt acagaaatcc aagaaattcc
    30301 cgtcatcgtt atgaattata atgatgaaga aaccttatta attttattgg cgaaagcgcg
    30361 tgatcaactt ggtactaatg ttttagaatt agcgtatatc tttagtgttt tatcgacaaa
    30421 acacggctat tctcaccaag cacttgcgga tttaacattc agaagcaggt cgcaggttac
    30481 aaatatttta cgacttttaa agcttgagcc agaagttatt cgtgcgctta ataacgagca
    30541 aataactttt ggacatgcac gggctttaat ctcatttgat cgcgaaaaaa gtcttgaata
    30601 tttagaatta attcgtgtta ataatctctc tgtccgtgaa ttagaagcct taataaaagc
    30661 accaaatact gcgacatata atggtaagga ttattttgtt attcaaatga aaaataaatt
    30721 aacaattgtg tttaataccg aagaagaagc actagcgttt aaaaacaaac attttaaata
    30781 atcatttatc ttgaatttat taccgataac tactattata aaagtacttc agggcggggt
    30841 gaaagtcccc accggcggta taccccgcga gcgcaagctg aactagtgaa attctagtgg
    30901 cgacagttaa agtctggatg gaagaagttg tttcttttat gtgtagccct gaatagagag
    30961 ggctattttt tatggaaaaa gagaaaaatt acaccaattg tcaaaatgat aatttagaaa
    31021 ctaatgcaca acaaaaatgt aaacgcagaa gaacagttac tgtcgctggt acggcaatat
    31081 ttgccgctat ttcagtctta ctttatatta ctaaagttcc cccgtttgtg ttcccaattt
    31141 tcccgcttgt taatttttta gaaattaatt ttagtgaaat tcctgcactt attgccggct
    31201 ttgcttacgg gcctttaagc ggattccttg tgttattagt gaggtttcta gtaaaactac
    31261 cgatgtcatc aactgcaatg gtcggtgaag tcgctgatat tatatattca tcgagctttg
    31321 tccttactgc cacaatcata tataaatatc ggcggacttt caaaggggcg cttattggtt
    31381 taggcatcgc ctttattgcg caaattttag ttagcagtct tgccaattac ttcgttattt
    31441 atcatcttta caatgaaatg ttttttaacg gtaaaatggc aatgatttta agtaataaat
    31501 atggggcttt tacaccagag caatttattt tatgggtcat cccttttaat tttattaaaa
    31561 atacaattat cggcggacta acactcgttg tatataaacg aattagtcgc tttataaatc
    31621 agttttatta gcataaaaaa accagcaccc gaagtggctg ctggtatttt tttaattctc
    31681 ttaacttatt cgacggtgat tgcaccagtt ggacacgaag cttcggcttc agctgcagca
    31741 tcttctaaat ctgctggaac ttcttcaacg agagctttga gttttccgtc atcatcccaa
    31801 tcgaaaaggg aaggacaaac tgcgttacaa acgccacaac taatacataa atctttatcg
    31861 agtttaactt tttttgccat aatttcctcc tgatgaataa tattataact atttgagagt
    31921 atcaattcaa atcatattgg aaacgataat tataaaattg tcgtgtaagc attttatgtt
    31981 tatttatatt attataaata cagtatggca aagcgtagag aaagtaaaac gagagtagag
    32041 tactgggcaa aatatcgtga gagtattacg aatgattttg aagttgaaca aaaaatgcgt
    32101 gaatcacgga aaaaacaagc tgaaaatact cctattaaaa agactgaaac accaccgctg
    32161 acaaaggctg aagctttact tagtgaatat gaaaggaaaa atgaaaataa agaaagcgca
    32221 gtaaaaaaag gaccaatagg catattatat gggctagcta ttatcgtatt gctccttatt
    32281 attgctggta ttattttcat gattttaata tatgggaata aataatattg ctatcgctat
    32341 tgatggacct gccgcagcag gaaaaagtac aattgctaaa aaagtagcga atcattacaa
    32401 ttttgtctat atcgatactg gtgctatgta tcgcgctttt acgtggtacg tattaaaacg
    32461 cggtgttgat ccaaaagatg aaacggcatc aaatagttta attcctgaag taacgataag
    32521 gttaactgct gattataaag tttttgttaa tggtgaagat gtaagtaaag caattcgtga
    32581 acctagtgtt tcaggcaatg taagctacat tgcttcatat cccaaaattc gtttattttt
    32641 aactgaacaa caacgggaaa tggctaaagt taatagtgtc gtaatggatg gccgtgatat
    32701 tggtacatat gttttaccag acgcttttgt aaaaatattt caaaccgctt ctgttgcagt
    32761 gcgggcgaaa agacgatact tagaaaattt agaaaaaggg attgccacta gtttagaaga
    32821 tattgaaaaa gaactagcaa tgcgtgatca tattgattca acaagagcaa tggcgccact
    32881 taaaaaggcg gatgacgcta ttgagttaga tactgacaac ttatcaattg atgaagttgt
    32941 cgctgaaatt atcaagatcg ttgataagaa attaggagtt aaataatggc attaccagtt
    33001 gtggcaattg ttggaacacc aaacgttggt aaatcaacgg tttttaatcg tatcatcggt
    33061 gaaagacgag cgataattca tgatgaacgc ggggtgaccc gtgaccggct ctatggaagt
    33121 gctgagtggt taacgcgtcg ctttaatctt attgatactg gcggaattga aattgctaag
    33181 acagcattcc aggaacaaat tagagcgcaa gttgaaattg cgattgaaga agcggatgtt
    33241 attgtttatg ttggggacgg tccgcttggt gttacaaccg acgaccgtga aattgctaat
    33301 ttactatatc gcgctaataa gccagttatc gttgcggtta ataaaattga cggaattgaa
    33361 catcatcatt acatccatga cttttatagt ttaggcttag gtgagccaat cgctatcagt
    33421 ggtgaacacg ggattgggat tggggactta cttgatgaaa ttattaaagt attaccggaa
    33481 caaaaagatg attatgaaga ggatgtaatt tcttttgccg ttattggcca acctaatgtc
    33541 ggaaaatcaa gtttagtaaa cgcgatacta aatagggaac gcgtcattgt tacaaatgtt
    33601 gaagggacaa cgcgtgacgc gattgatacg ccttttacta gaaacaataa aaattatgta
    33661 gtaattgaca ctgctgggct aaaaaagcgt ggcaaaattt atgaaagtgt cgatagatat
    33721 gcagcgcttc gggctttcag cgcgattgct aaagctgata ttattttatt tgtcattgac
    33781 gctgttgtgg gtattcgcga acaagataaa cacgttgttg gttatgcagt tgatgaaaaa
    33841 aaggccatta ttattgctgt taataaatgg gacgctatta ctaaagacga caaaacaatg
    33901 aatgaatgga caaataaaat tagaaatgaa tttaaatttg ttgaatatgc tccaattgtt
    33961 tttgttagtg ctttaactaa acaacgagta gatcaaatct ttgaccaaat tgactatgtt
    34021 aatgaagcgt atcgtcgacg tgtagagaca agtttactaa atgaagttat ccatcgcgct
    34081 caattgtata atgacccgcc agtgtttaat ggcggtcgct taaaagtgta ctacgctaat
    34141 caagtaagca ctaaaccacc gacatttgtg ttctttgtga ataaccctgc ttttctacat
    34201 ttctcttatg agcgttactt agaaaatcaa attcgtgact cgttcccgtt cgatggtaca
    34261 ccactgaaat ttatattccg tgagcgtaaa tgagattaaa catttgttca tttttagggt
    34321 tgttttaagt gtaaaaaaat caataaagtc gtaaggcatc gcaataattt tcaataaaat
    34381 attaaatatt tcttgtataa attatataat tatgctactt tgtaaaaaac aaaggagtga
    34441 gaaaataata tgaacaaagc agaaataatt gctgccattg ccgatgagct tatgattact
    34501 aagcgttctg tggaagaaat tattaattta ttttgtgaca cagtcatcga aaccttagcg
    34561 aaaggtgaag atgtaaaact tacaggtttt ggcactttcc aaacaagaga acgtaaagaa
    34621 cgtcgtggcg ttagcccaca aaccggtaag gaaattgtta ttccaagcgg ccgtactatc
    34681 gttttcaaac cttcaaaact tctcaaagaa aaagttcaat aaataactct ttgctagaga
    34741 agtatgttga aatcatattt agagtttttt gacgagattg ctaatatttt caacaagcac
    34801 ggctttaaac tctacatggt tggagggaca agccgtgatt ttcttttaaa ctacccggtc
    34861 gacgactatg attttgcgac agacgctaca ccaacggaaa tgcatgaatt tttgcctgac
    34921 gcaaattatc ggtttgaaaa gtttgggaca gtaagtttaa agtatgaaga tgaacatgtg
    34981 gaaattacaa ctttgcgcga agaaggtgaa tatattgata gtcgccaccc cgcttatata
    35041 aagtttgtaa cagaccccgc taaagattat attagacgtg attttacgat taacgcactt
    35101 tatattgatg aaaattatcg agtgcttgat ttttgtgatg gcttaaaaga tttagataca
    35161 aatactttac gcgttattgg caatccttat acccgttttg cagaagatcc gctgcggatt
    35221 attcgggcat tgcgatttgt tgctaagttt aaattaaata ttgacccctt aactgagaac
    35281 gcgattcgtg atttagcgca tcttgttaaa aaactaaatc cagaaaaagt taaagctgaa
    35341 atacgaaaag tgggagaaca agagcaaaat atctttaaaa aattaatgaa tatgtacaaa
    35401 ttataagatt tacattgcgt aatagtgtaa actattaata ccaaaagtga gggaatcttt
    35461 atgagtggat ttataggaag agttggtaat tcgtgtgtaa aagagaaact gttaagtgcg
    35521 ttaaacttac ttcagttcaa agatgatgac tccacaggag ttcttttttg caataaagaa
    35581 ggccaacatt tatatcgtgt agtaggaaaa gtagatgaat tagtcacact tattccggaa
    35641 gaagttgatg gttctatggg gattgctcac actagatggt caacatacat gcatcaagtt
    35701 cttgaaaatg tttatccgct ctctagtgaa aatggttatg tcacccttgt tgttaatggg
    35761 cttattgata atattcaaat gattaagcgt cgacttgtaa aacgcggata tagttttaga
    35821 tctagttccg ctgttgaagt tgtcgctaat ttaattgaag acggcgctgt tgaaaacgaa
    35881 gacccactac ggacactcca tcgcgctatg caacaactag aaggtgatta tgccttgctt
    35941 gctgtcttta acggtaattg taagaaaata ttttttgcta aaaatggtgt tccgctcatt
    36001 attggaaaga atcatgacgg gatggtcatt tgtacagatt acaccccaat tgccgatcaa
    36061 gccgatgaat attattatcc aaatgacgga gagttaggct atttaacaag tgataatatg
    36121 gttttatatg acggcaaact taaacaaaga aaaccgtctt ttaccgaagt gtcagttagt
    36181 gaaaaaacac ttgagttagg tgattatcca cactatatgc ttaaagaaat tgaagaagct
    36241 cctggtgtta ttcgtcgcct tatcaaccat tactttgatg gaacgaaata tcggtttgat
    36301 gaccggctta ttaaacgaat tattgatgcg ggaacgattg tctttattgc ggctggtact
    36361 agttacaatg ccgctttagt gggacaacgt tatctacgaa actttaataa gaaagttgac
    36421 gttttcatcg ctagtgaatg gattttttat ccgtatcaat caggtgaaga cacattatat
    36481 gttttaattt cacaatcagg tgaaacagcg gatattctta aatgtttacg cacaattaga
    36541 gcatatggcg ctgatgtttt agcaattact aatgttgaag tttctacact ttatcaatca
    36601 gcggattatc gcttattact acacgctggt acagaagtta gtgttgctag tacaaaggct
    36661 tatatcgcgc aaattacact tctttctttg ctttacgcgc gcttagtaaa taaaattacg
    36721 acaattgcag cattagaaag agttattgaa gcgctctata acattatctc gcgtcgtgat
    36781 acgattaagg caattgccga tcaaatttat aagtcaggcg acgtcttttt cttagggcgc
    36841 gggtttgact atgatgttgc tgtagaagca caacttaaat taaaagaaac tagctatatt
    36901 catgctgaag cttatccggg cggagaaatg ctgcatggcc caattgcgct tattgaagaa
    36961 aatacaccag ttatcttctt tattagtgat agtttaactg tagcagcaac gcgggaagtt
    37021 attagagaaa tgcgtgatcg taaagctaat gtaattgtat gctcacttgc gccatttaat
    37081 catgatggtg atagctttat tatttcaaac ccaattaagg gttatcatag tccaatcacc
    37141 tttgcagtat tcgcgcagta tcttgcttat tattgtgcag ttaacttagg acgtgatgtt
    37201 gataagccac gtaatttaac aaaggcggtt aaagatcgta gtgtataata aggcgctaga
    37261 ttttcgcttt atgctttttg aatattacaa agagctccgt atcaatgaaa cagaacttgt
    37321 aatcattctt tttattgagc acttacaaca aaaagaaaca gaatttatta ctcctgattt
    37381 aattaatctt caatcaaata ttgatatcaa agtgattgac gaagcaatgg ttcgtcttgt
    37441 tagtaaaggt ttaattgaat ttacgactag aaacgggaaa atggttactt cacttaatcc
    37501 gttacgaaat aaattacaag aacttttcgt gttagattat gaaaagaata aagaaaattc
    37561 gacagtagaa tttagttcac aagttgaaga agtttatgcc tttattcaaa atgcttttgg
    37621 ccgttcactt tcagcagtag aactgcaaac gattaatgat tggtttaatt atggttataa
    37681 catcacaatg attaaagaag cacttgacca aacaatgaaa cgtaaacgct ataacattcg
    37741 cgctattgat aaggcgctac ttaaattatc gactgctgtg gattatgcgc ttgaaggtaa
    37801 aagtgcccaa agtgattcat atcgaaaaaa catcaaggac acacttgaag aagtgaataa
    37861 aaaagcccaa gatgacgaga acgaataaag aaagaatact cgcctttctt gataaagaat
    37921 atagtgacgc taaaggtgag ctagaataca caaaagatta tgaactgctt attgctgttg
    37981 ttttaagcgc acaatcaact gatgtagcgg ttaataatgt tacgcgaaaa ttatttgcaa
    38041 agtatccaac ttttgaaagc ttattaaatg taaaagaagc acaatttgcc gcgtatttta
    38101 aaacacttgg cttatatcgc aacaaagcaa aacatgtgta taatttaatt catttactta
    38161 atgaacatca tagcggagtt gttcctagtg ctaaagacga gcttttaaaa ttacctggtg
    38221 ttggagttaa aaccgcgaat gttgtacgcg ctgaactctt taacattccc gaaattgccg
    38281 tggacactca tgtttcacga atagcgcgtc gccttggttt tgctaagttt agtgatgatg
    38341 taactatcat cgaagcgaag ttacgtaaag ttttacctaa agaaagatat ataaaaacgc
    38401 atcaccaaat gattcatttt ggtcggtatt tttgtaaagc acaaagtccg ctatgtaaaa
    38461 aatgtccgct tgtggacatt tgtaaagaac caaataaaaa gttataaatt aaataatttt
    38521 atgactgcag gcaaatagtc aagtacaggg ttaagaccaa ggcgcactaa tacgcctttt
    38581 tctttaaatg atgcataagg gattgattta cggtcgccat tgcgataaaa atcaataaca
    38641 tcagcagcgc taagtacata atattcatca aaagctgtaa attcaacaat aaagaaagca
    38701 atcccaccat ggcgaataac attttctaag tgagtaattt gatgttcata aatattattt
    38761 aaaggaaaac ttgtttttaa tcttgtactt ttagcttcaa agtcgaaata tcggttttta
    38821 taaaccccgt taaagtctgt tgttgattgc ttttcaaagt aggcgtttgt aatttttgcc
    38881 cctttagcgt aatcaatttt tacgacatta attggtgttg ggcgtttagt aaaaagggca
    38941 atgtcgtgca tcaaataata gtcattagca gcactgataa gtttttcaag cgccatgcca
    39001 cgattagcag cgctcttcgt aatatttttt cgttttgaag gtactttcgt tgtttgtttt
    39061 ttaggtttag aaccgtttgg ataattaatc attacacaat ttcatcgcgt aaatattctt
    39121 gtaaatcatc aatatttaca ctttctcctg cctttatttt attgagcatt ctttttattg
    39181 gacgcgggat attattcata ttgagtagta cctcaccata ccgttcaata agaatatcag
    39241 tctttttaaa gagctcacta tataaatagg cgagcatcag ggcatcattt aacgggtcat
    39301 gagcaggttc aactggctca acgccgaaaa gtttacataa attcaccaaa gataatgtat
    39361 tatttttttc atcttttata tattcattaa tgtatgcact taaatcaata tgatttttcg
    39421 caatcgtttt aataaaagta tgatgagctt caggagtatg ggcaagtgat tggcgtaaga
    39481 tacgaatgtc gtgtgcccca aatgtcacaa atttagtttt tgaaagatta gtgccaacat
    39541 attttttaag catcagtaga atcttatcaa aaggaatgcc ttctcttgct aataaatcat
    39601 ccgtaatgcc agttaatttt tcaatataat taccaacatt ttgtgttgct agcacataat
    39661 gtttaaagcc aggataaagt tttttaatcg ttccgtccgt tttaattgtg gctttaattg
    39721 ccccaattgc tattagttca tgactaaatt gtgtaccttc taaatcaagg aagataagtt
    39781 gcttgcggcc acgtgttgct ttcgctaatt ttttcatact ataaaattat aacatagtaa
    39841 taataaagcg gtctcacttt gttgcgtcaa aacaaaatta catatataat atgtatgcga
    39901 ggttaacgat ggcagaaaag agattactag acgcagacaa aattttaaaa aagaagttca
    39961 aagcgaaaag cggtggctat gacgctttag aagttgacga attttttgat ttagtgcgta
    40021 atgactatga atctatgctc gaaattgaaa aagaattaga attactgcgg ctgaaaaatg
    40081 aaactcaaca agctaagatt gttaatcttg aagcgcaata cattcaatat aaaaagaaag
    40141 ttgaagagct agaacgctta attagtaaag gcggaaccgc gatggaaaac ttacgtaaaa
    40201 ttgataagta tgagcgtcaa ttatggaaaa tgggcattga cccaagcaaa ctcaaataaa
    40261 attcgatctg gataattgct ggtacataag tattagagga aagtccatgc tcgcacttgc
    40321 tgcgatgcaa gtagtgattg cgctagtgga aaaaataaca ctaggtatag aggagtctat
    40381 aacggcgaag aaagacctaa gtcgaaagat atggttaatc ttctgaaagt gccacagtga
    40441 cgaagttaag gggaaactct taagtggaac gttggtaaac cccgcaagcg agaaacctaa
    40501 atttggtaag ggaaacggca atagagaaat gaatttagcg ccgtatgtgt tcacacatag
    40561 ataaataatt atccgcgaca gaacatggct tacaggatcg aaactcacct aaaggagcat
    40621 tatgaattta ccgaataaat taacagtagc gagaatgata cttgtcatcc ttttaattgc
    40681 ttttttactt attccgccca cttggttagg taatgaacca attattcttt tttctaatgt
    40741 ttatctgcgt tattttattg ccgcaattgt ttttatagta gcggcaatca ctgattactt
    40801 cgatggatac ttagcgagaa aatttaattt aattacaaat tttgggaagt ttatggaccc
    40861 aattgccgac aaaatgctgg tgaattctat gttaatcatc cttacagttc cgcatcaagc
    40921 gttcgtcggg caaataagtg tacctgttat ttttgttgta ctcatgattg cgcgtgatac
    40981 agtagtggac gggtttagac ttgttgctgc aaatcaaaat aaagtactag cggcgaatat
    41041 ttttggtaag gctaaaacag ttttacaaat gattgcaatt gtgatgtttt tatttaatga
    41101 ttggcccttt tcacaccaaa tgcaaagtga ttcgtttccg attgttagca tgatagtaat
    41161 tggcgctgct acatttgtgt cattattatc aggctttatt tatatattcc aaaatatcag
    41221 tgtacttaag gagacgcatg acgacacaac tgctcattaa tgaattaatt gataaaaatt
    41281 taacaattgg tagtgttgag tcttttactg gtggtttatt tgcggcaaca ctcgtgaatt
    41341 atccaggtgt tagtaaaaca tataaaggga cgattgtggc ttatagtaac gatgtcaaag
    41401 agcgtttact taacattaaa aaagaagatc taaaaaaatt tagtgccgtt tcaagtgttg
    41461 tagcgcttga tttagcgcgt catggccaaa aagcattagg agttgatttt tgttttggct
    41521 ttacgggtga cgcaggtcca acagcatcag gccaaagcgg agaagttggt gatgtttata
    41581 ttgccctagc gtttgctaac gacgaaattg ttgaacactt caaatttaaa ggtgatcggc
    41641 ttaaagtccg cactgatgca gtgaatgctg ccttaaaaat gttagcgaga gtgttaaaaa
    41701 acttcgaata atattgtata tatctatata aggagaaaat tattatgtca aaaaagaaag
    41761 atgcaaatat tttacaagaa acattaaaag aaattgaaaa gtcgtttggt aaaggcgcca
    41821 ttatgaaact aggcgaacgc ccagttatta atgttgatgt cattccgagc ggctcattat
    41881 taattgatga agcacttggc gttggtggct atccaaaagg gcgtattatt gaaatctttg
    41941 gaccagaaag tagtgggaaa actactttag cgctgcacgc aattgctgaa tgtcaaaaga
    42001 aaggtggtca agctgctttt gtcgatgctg aacatgcaat tgacccgatt tatgctaaaa
    42061 atttaggaat taatattgat gaattacttt tagcacaacc tgattcaggt gaacaagcac
    42121 tggaaattgt tgtccgtctt gccgaaagtg gtgtgattga tttaatcgtt gttgatagtg
    42181 tggctgcact tgtacctgaa gcagaactat ctggcgaaat gggtgataac caagtaggac
    42241 tacaagcacg cctgatgtca aaagcaatgc gtaaactagc gggtgtatta aataaagctg
    42301 agtgtactgt tatctttatt aatcaactac gcgaaaaggt tggaataatt tatggcaatc
    42361 ctgaagttac ttctggtggt cgtgcattaa aattttattc ttccattcgt attgacatta
    42421 gacgcggtga agcgattaag caaggttcta aattaattgg taatcaagca aaaattcgta
    42481 ttgtaaaaaa caaagttgca ccaccatttg ccgcttgtgg aatcgatata atttatggca
    42541 aaggtatttc taaaattggg gaaattcttg aattagcggt taaccgcgga tttattaaaa
    42601 agtcgggttc gtggtatgaa cacgatggac ttaagattgg acaaggccgc gaagcagcgc
    42661 aaaattatat tttggagcgc ccagaattac tcgctgattt agaagaaaaa attaaaacag
    42721 gcgtagtcgc cactgaaact aaataattac aattatttta taaattttaa aattagaaaa
    42781 cacaattttt aaaattgtgt actttactaa tcatctttgc tttaattaat gctaaaaagt
    42841 tgttataatc aaagtgcgca ctaagtgcgt tcacctaaac ttcatgttag gttattaatt
    42901 cactttattg aaatttattt acataaatca tcgctaaaca ttaatgatgt aacattggaa
    42961 tttaggtaac agactatttt tcgtttgtat atattttcaa acacaaaatt ttaaaaaaca
    43021 aagaaagaag gaacacaaaa tggaagtttt gtactatgca ctattctttc ttgtcggact
    43081 tggacttgga atcggactat ttttcctcat tccagcattt aaagcgaagc aagcatttgc
    43141 gaaagcacaa aaattaacaa aagaagcgga aattaaagcc gaacacattg taaagaatgc
    43201 tgaacttgac gctaaacaaa tcatttatga aggaaaacaa gaactcgaac aagccggcaa
    43261 agaacggcgt gcccaaattc gtgagctaga aaggctcctt aatgatcgtg aaaaaggaat
    43321 tgaacgtcgc gatcaattat tacatagcaa agagcagtca cttgacgctc gtcatgaaaa
    43381 cttatcagtg aaaatcaaag aagctgatga actcaaaaaa gacctacaaa ttaagattga
    43441 ctcaatttta ggcgagcttg aaacagtcgc acaaatgaca gttagtgagg cgcgagatga
    43501 attatatcat cgcgttgacg ctaaactgga aaaagaaatc gcgctgtata ttaagcaaaa
    43561 agaagaagaa gccgaactac aagttaatga acaagctcgt gatattttag caaacacgat
    43621 tgcgcgttat tcacaagaag tgacgactga acgcactgtt agtacggtcg aattaccaaa
    43681 tgatgaaatg aaaggacgca ttatcggtcg tgaaggacgc aacatccgca cacttgaaca
    43741 actattaggc gttgatattt taattgatga tacaccagaa gttattactg tgtcatgctt
    43801 tgatccaatt cgccgtgaga ccgctagacg gacgattgaa ctattagtca aagacggccg
    43861 aattcaacca agtcgtattg aagaagttgt ggaaaaagca acgcaagaac ttgatgaaat
    43921 tattattaag actggtaaag acgctcttgc cgaacttggg attcctaatg tccacaaaga
    43981 tatgattcca tttattggcc gcttaaaata ccgcacgagt tacggacaaa gtgcacttaa
    44041 acatagtatt gaagtcgccc atttatgtgg cattatggct gctgaattag ggcttgatat
    44101 tacgcgcgct aaacgtgcag gattattcca tgacattggt aaatcagcgg attttgaact
    44161 tgaaggaagt cacgttgaag ttggagcaag actagcgaaa aaattcaatg aaccagatta
    44221 tgttattgat gcaattgaaa gccaccacgg tgataaagaa tcgcgtttta tctttagtca
    44281 attagtcatc gctgctgata cattaagtgc agcgcgacca ggtgctcgga ctgaaacgct
    44341 tgaaaattac attaaacgtt tagaaaaact tgaagaaatt tgtaaatcgt atgacggtgt
    44401 gcaaactagc tttgcaatgc aatcaggccg tgaagtacgc gtgatggtcc atcctgataa
    44461 ggtggatgat attatggtca ataaactcgt tcgtgatatt cgtgaacgga ttgaaaatga
    44521 aatgacttat cctggtcaaa ttaaagtgtc ggttattcgt gaataccgtg caagtgatgt
    44581 agcaaaatag taatggtgaa aattttattt attggtgata ttgttggcaa aatcgggcga
    44641 gaaactctcg cccatttcct gcctatactt aagcgtgaac atgactttga tttaattatc
    44701 gctaacggcg aaaatacaac gcatgggcgc ggattaacga aaaaacatta tgaagaatta
    44761 gtaaatcttg ggattgatgt catcacactt ggtaatcatt ttgatgatcg tccagaactt
    44821 aaatattaca ttaccgaagt gcataattta gtgagaccat taaacttaat tgaaaattat
    44881 cctggtgaag gatctgtcgt tgtcacaacg aaaaaaggtg acaaagttcg cgtcactaat
    44941 gtattaggac aagcatttat gaaagaaatc attactagtc cgtacgctaa tcttaaaatg
    45001 cttaacgctg atacagaaaa gatgattcat atcgttgatt atcatgcaga agctactgca
    45061 gaaaaaatga cgattggctg ggaatttgat ggtgcagtaa gtgctgtttt aggtacacac
    45121 actcatgtac aaacgcgtga cgcccgtatt ttgccaaaag gtacagctta tattagtgat
    45181 gttggtatga cgggggcata taatggcatc attggtgcag atagagaacg cgtaatcgag
    45241 cggacttggt ttgataaaca agtaccgttt acttatgagt acaaagataa agaagcgcaa
    45301 tttagcgctg ttattttaga aattaataaa catacattta aaacagaaaa aataacacca
    45361 ctttattact tatggagaag agatgagtaa aacattactt aataatttac cctccttcac
    45421 taaagcacga agacgttatc cgtccatagt tcaaaatctt aagattgaag taccgtcaat
    45481 tgttagtgcg aagttaagtg gtaaattttt cttaattaaa acatttggct gtcaggcaaa
    45541 tgtacgcgac gaagaaagta tggccggtct tttaagtatg gctaatatgc gacgcactga
    45601 agaagaaaaa gacgctagtg taattattat taatacatgt gctgttcgtg aaggggctga
    45661 acaaaaaatc tttggtcaag tcggtaactt aaaaggacta aaaacaaata ataaagattt
    45721 aatagtaatt gttgcgggtt gtgttgtgca acaagaagaa tcactcgcga aattacgaaa
    45781 aacattccca tatgttgata tttttatggg gacaaacgct attcataaat tacttgattt
    45841 aattgcctta cgaataacgg aaaataaaaa agtagtggat gtgcctagtg atgatgccga
    45901 tgtctatgaa aacatccctg tgcaacgcat tgccccgcat aaagcttttg tcaatataac
    45961 atatggttgt gataagtttt gtacatattg tatcgtccct tacactaggg ggaaagaacg
    46021 ctcacgatta cctgctgata ttttacgaga atgtcaaatt ttagtggatt ctggttatca
    46081 agaaattaca ctcgttggtc aaaatgttaa tgcttatggt attgatttaa aagatggcac
    46141 aacttttagt tatattttag aagaagtagc aaaacttggc atcccacgcc ttcgttttat
    46201 tacttcgcac ccatggaact ttgatgatga aatgattgat attatcgcta aatatcctaa
    46261 tatcatgcgt aaaattcatt taccgttaca aagtggtagt gatgaaattt taaagttaat
    46321 gggacgccgc tatacggtta aagattattt agagttagtt actaaattac gggctagaat
    46381 gcctgatatt tcactgacta ctgatattat tgttggcttt ccaaatgaaa cagagcaaca
    46441 atttttagaa acattaaaag ttgttgaaaa ggtaaattac gaaaatgctt atacttttat
    46501 ttattcaccg cgcaagggta ctcccgctgc ccttatggaa gataatgttt cgcgtgaaga
    46561 aaaaggtgag cgctttaaca agttaaaagc actcgttgat agccaaaccg aagctaatgc
    46621 agcgctatat gtcgggaatg agtatcttat tttagttgat ggttacagta aaaaagatac
    46681 gagtgtatta agtggataca ccgaaagtga tcgtgttgtt cattttgtag gtactcctga
    46741 tttaatcggt aaaattgtta aagttaaaat tactgagtca cgcgtttatt catttgttgg
    46801 agaactagtg aatgaataaa gttgaaatag ccttaaatgc attaacgact gaattagcga
    46861 atgacaaaag agtcgtagaa tttaaaaaag taaaagcact gattgaaagt gatgcttact
    46921 taaaaaatgt agaagtacgg cttaaagagt tacaacgatt aatgacacaa aacgccttta
    46981 acgaagaaaa gcataacgag tataaaagag aatatttacg actaaaaaac aattatgaaa
    47041 cgcatcctta tctaataaat tataatagtt tgctaagtga aattgaagat ttactttact
    47101 ctttaaaaac agtaattgaa taagcaattt accatactat ggtaaaatga cactatggga
    47161 aaagagaaga aaaaaatcgt ctatactccg atgattgagc agtatttaga aattaaacgg
    47221 gaaaacccgg gtattttaat tatgtatcga cttggtgatt tttatgaatt cttttttgaa
    47281 gatgctgaaa ttgtatccaa agaattacaa ttagtgctca ctaaaagagc agcaggaaat
    47341 aatcgtaaaa tcccaatgtg cgggataccc catcacgctt atggtccgta tcttgccaag
    47401 ttaattgacg ctgggcataa ggttggaatt gttgaacaaa tggaagatcc tagttcagca
    47461 aaggggattg taaaaagaga aataatccaa attgtaactc ctggttcgat tgtcgatttt
    47521 gaagatagtg ataataactt tatcacagcg gttgatgctt acgctgatgc ttttactgta
    47581 agttacgcag atgtttctac tggtgacatt ttcgtcacaa ctttaccgcg tgaagatacg
    47641 agtttagtaa gttatttaaa cggattaaac actcgtgaaa ttgttgtgag tccacagttt
    47701 agtgataagc ttttaggtgt tattaaaaca aatataaatc ttgttatatc gtttgaagag
    47761 aatacaaaaa ttgatgaaga atatgaagat ttatttagtg atgttaagga tgagcgggag
    47821 tatttgattg ccgcgcgttt agtgagttat ttaacacgaa ctaaaaaacg acaattatca
    47881 tattttaaag caattactgt gacaaaaccg cagtctttct tacaaataga tgctttttca
    47941 cgccttaatt tagagttaac aagaacaatt cgtaatgaaa caaaatacgg gtctctattt
    48001 tggctattag atgaaacaac aacagcgatg ggtcgtcgta aactaaaaga ttttattatc
    48061 cgtccttcac acgattatga tgaaattatt cggcggcaaa atattgtaaa cacatttatt
    48121 gttaactttg taacacgtgc ggaagttaaa gaacattttg ttagtattta tgacttagaa
    48181 cgtatagtgg cgcgcatctc ttttggtagc gctaatccgc gtgacttgtt gtgccttaag
    48241 acttcactcc aagcactaga aaaagcacgt aatcttttag gtaaaattag agaacctgaa
    48301 attagtgaac tctatgataa gcttgcaagt aatattcctt taattacctt actggaaaaa
    48361 gcaattagtg aagacgcccc actacaaata actggtggcg aaatatttaa gtatggatat
    48421 tatgaagcgc ttgatgaact aattgattta gcgcatggtg gtaaaaaatg gattagtgct
    48481 tttgaagaaa aagaacgagt gcggacagga attaaaaatt tgaaaattgg ttataatcgt
    48541 gtatttggtt attacattga agttagtaat agcaatttag atcaagttaa agatgaattt
    48601 ggttatatcc gcaaacaaac aacaaagaac ggtgaacgct ttattaatga agcgcttaaa
    48661 gaacaagaaa cactaattct aagtgctgaa gaacgacggc aacaattaga aagagaactt
    48721 ttcggtgcgc ttttaaatga gttaaagaag tcaacacgat tattacaaca aagtgccgat
    48781 gttgtaagtg aaattgatgt ttttatttct ctagcggaag tggcggtcgc taataattat
    48841 gttcgtccta tatttaacga tgcacgcgtg gttgatatta aagaagcacg gcaccctgtt
    48901 ttaagtaaat tacttaaaga ccacgccttt gttgcaaatg atattgagct agatgaagcg
    48961 accgatatcg tgattattac tggtccaaat atgggtggta aatcgacact tatgcgtcaa
    49021 ttagcgctaa ttgtgattat ggcgcagatt ggcagttttg ttccggcaaa gagcgcaaac
    49081 ttaatgattt ttgatcaaat ctttacacgg attggggcaa gcgatgattt aataagcgga
    49141 caatcaacat ttatggttga aatgattgaa gcaaattatg ctttacgcaa agcaacacca
    49201 aattcacttt tactatttga tgaaattggt cgcggcactt caacttatga cggaatggct
    49261 ttagcctacg caatgcttga atatattgct aacaagttaa aagtaaagac attatttagc
    49321 acgcactatc acgaaattac tgcaattgat gagcattatc ctaatattaa aaacattcat
    49381 gttgaagtaa aagaagtcga aaataatgta accttccttt ataaaattaa acctggtcct
    49441 atgaataaga gttacgggat taatgttgcc cgtttagcgg gattaccgct agaaatttta
    49501 agtaatgccc aagaacattt acaaacattt gtcgctaaca atagtggcgg caatccgact
    49561 aaaacagaat atgtgccact aaaaccatta tggtatcaac agctcgaaaa tttaaatatt
    49621 gatgaaatga ctcccctaga tgcccttatt cttttaaaga aaattaaaga tgaagggttt
    49681 aaataatggc taagatcatt ttactagaca acgaacttgc taatcaaatt gcagcggggg
    49741 aggtaattga aagaccaagt tcagttgtta aagaattagt ggaaaatagt attgacgctg
    49801 gtgctactaa aattgatgtt tttattaatg aagccggacg taaagcaata attgtacaag
    49861 ataatggtag cgggattcct agcgacgaat tagcgctagc tttttcgcga catgctacaa
    49921 gtaagattca ttcaactttc aatttattta gaataagaac tttagggttt agaggtgaag
    49981 cactaccaag tattgcttca attgcaaaag tgcaagttga aactgctgaa aaggggaaac
    50041 ctggagcact tgctaagatt gaaaatggtg atattaaaat tgagccaagt gcagttagta
    50101 gtggtacaaa aattactgtc tctgaacttt tttacaatac accagcgcgc cttaaatatt
    50161 taaaacgcga ttataccgaa aacgctgtaa cacttgatgt tgtgactaaa atagctttag
    50221 cccatcctga tgttagtttt agtttaacat ttgatgataa gttaagattt cgcacaagtg
    50281 ggcgaaataa tttagaagaa acaattttaa atatttatgg tttaacatat gttaaaaact
    50341 tattaccaat tagtgttaaa acgaatgatt ttacgattac aggtttctta gggaacacga
    50401 atttagttcg ttctaatcgt tacgggatca ttacaaactt aaatggtcgt aatgtatata
    50461 tgccgaaagt gcaaagtacg ataattgaag cgtatcacgg ctttattgct cccacgcgtt
    50521 atccgtttgt cattttaaat ttacaaatag aaccagaatt agtcgatgtt aatgtgcatc
    50581 cgactaaaaa agaagtgcgt ttttcaaaag aagaagactt acgcaatgtt ttattaaaag
    50641 aaattccgct agcgctccgt aaagaagcat ttatccctaa agatccgcac caaaaagtgc
    50701 aaccactatc aagcactact aacgaagaac ctagagccga gcaacttatt tttgatttgc
    50761 cgtttggcac tgaaactagt gaattaaaaa ttagtgatag tcatcatcaa gaagcaatta
    50821 gaaaaccaat aactaaaaca attagtgaat tagaaaatag ttttgttaat ccagttgcag
    50881 cactccataa cacctacatt atttgcgaag taaatgatgg gggcttttgg cttattgacc
    50941 aacacgctgc tcatgaacgg attaattatg agaaatttca aaaagtatta aatatggatt
    51001 taaaaactag accactttta gtgcctgaac ttttatcttt tagtcatagt gaaatagcgc
    51061 tttttactcc tgaagtattt accattctta aagatgtcgg aattgaagca gagttatttg
    51121 gcccaaatac actaagagtt actgctgtgc ctagttgggc aggtgaatat gatgaacgca
    51181 tttatattca agatatcatt gatgctgtta ttactgataa gcgtttaaat caagagcttt
    51241 tacgcactaa cgctattgca acaatggctt gtaaagcatc aattaaagct aatcaccgcc
    51301 tttcaattat ggaaatgcaa catttaataa aagaattact agcgtgtgat aatccgttta
    51361 gttgtccgca tggtcggccg acaattatta agtttacgaa gtacgaatta gaaaaaatgt
    51421 ttaagaggac aggcacatga tttttgtcat tggtggtgtg accgcaagcg ggaaaagtga
    51481 tttagccttc cgctttgcta aagcaataaa cggaattatt attaacgctg atgcgtttgc
    51541 tttttataaa gagttaaata taggaactgc taaaccaacc aaagaagaat tagcgatgct
    51601 tccgactttt ttatttaaca atatttcatt agatgaacgc ttttctattt ataactatca
    51661 aaaagaagta cggattatat tagataagta tttagctaaa aaaattccga tagtgctcgt
    51721 tggtggcagc ggtctttata ttagagcagc cttatataac tataacttac aaccggaaaa
    51781 ggacttaaag attgttgatg accctaacat aagtaatact tcactgcaca aagaattaga
    51841 agtgttagat aaagaagctg ccgctaagat tcatcctaat aatcgtaagc gcgtactgcg
    51901 ggcactcgct ttaattaaaa ctcacgctca aacaaaaact gcgcttgaag aagtaacatc
    51961 aagtgagcca ctttatccgt atattatgat ttgtttagat aaagaaaatg aaatgctcca
    52021 tcgcgatatt gaaacaagag ttaagttaat gtttgacaag ggattaagag aagaagcgat
    52081 gacattaagc aagaaatacc cgcgaaatgc acagggaatg caggcaattg gttataaaga
    52141 gattgtgcaa aatcctaatg ccactgatga agaattaata aatttaattg ccttacgtac
    52201 aagacaatat gcgaaaagac agcgaacttt ttttcgtaat cagtttgccg ctaaatggtt
    52261 tactgataaa gaagcagcgt ttaattatct aagaggaaag tatgaggact cattatgaaa
    52321 aacacattac acattttaga agttgcaaag aaagttggtc tttcacctgc agatgtaata
    52381 ctttatggtg aagataaagc aaagattaaa agtccgttta ctgatattaa tccacaaggt
    52441 aaattaattt tagtaactgc gattacacca acaccttttg gtgaagggaa aacgacaatg
    52501 tcaattgctc tacaagacgc aatgtggaaa acgggaacaa agagtttact ttgtttacgg
    52561 gagccttcac ttggtccagt gtttgggatt aaaggtggtg ccactggtgc aggacgcgct
    52621 aagctttatc caagtgacga tattaatctt catttcacag gtgatctcca cgctcttact
    52681 tcaagcatta atctaattgc cgcagtaatt gataatcata ttttccaagg caatgcttta
    52741 aatttagacc cgaaacgtat cacatggaaa cgctctttag atatgaatga tcgtggctta
    52801 cgcaaagtta gaattaatca aggtacgggc aatggtactc ctcgctgcga agaatttcaa
    52861 attactgtcg ctagtgaact aatggccatt atgtgtcttg ctagtgatcc tgatgacttt
    52921 attcgtcgtg taaaagatat tattattggc tatacatatg aaggtgaaat tgttcgtctt
    52981 gccgctttag aaatagaaga tgcgattatg cacttaatga aacaagcatt attaccaaat
    53041 ttggtgcaaa cattagaagg caatcccgtt cttgtccatg gcggaccttt tgcaaatatt
    53101 gcccatggct gtaattcaat cattgcgaca aaatatgcgt taagtaaagc accagtcgtt
    53161 atcactgaag caggttttgc tagtgaatta ggtgcggaaa agtttttcaa tattaagtgt
    53221 cgtaccgcta atttaaagcc taatctagcg gtcgtagttg ccactattaa ggcattaaag
    53281 atgcatggtg gcgttccaat gagtgcaatt tatgaagaaa atactgatgc acttgccaaa
    53341 ggatttgaaa acttagtgca gcattatgaa aacttaagtt tatttggtgt taaagttctt
    53401 gttgctctta atctttttga taatgatcat aaaagtgaag tgaagttatt tgaaaaagaa
    53461 atgcaaaaac taggatatga ttatgcactt gttagctcgg tgagtaaagg cagtgaaggt
    53521 ggtattgaat ttgcaaataa agtaaaagca ttactagcgg aagaaactaa gttcgaattt
    53581 ctttatgata ctgagaaaga agttgaagaa aagattgaaa ttatatgtaa aaagttatat
    53641 cgcgcaagcg gggttaaata tagtaaaaga gcacaacgcg aacttgcggt aattaaagaa
    53701 caagggtttg ctaaattacc ggtgtgtatg gctaaaactc cgttatcatt tagtgataat
    53761 ccaaatttaa aaaatgcgcc acgaaacttc aatattacaa tccgcgaatt tagactaagt
    53821 gctggggcag gtttcatcgt tgccctaact ggtaaagtat taacaatgcc tggactccct
    53881 aaagtacctg ctgcggttaa tatgaaggag gttcgctagt ggctttaatt aaagttgatc
    53941 tttatgatga aattgaaatg aagaagccgc atccttgcat tcataagagt catcgctttc
    54001 aagtggtgat gcttggtgct gatttaaaat tgcaatgtca gggttgtgga aacatcgttt
    54061 taattagcag gattaagttt aatcaaaagt ttaaaaaaca cattgctagt cacgaaacga
    54121 taatttttac gaaataatgt tttatttgta aagtaggagg ttcctatttt acaagtcgtt
    54181 aatctatata aaagttttgg caaaaaagaa atatttagta atctttctta tacttttaaa
    54241 agtgggaaga tatatgtcat cgttggttat tcaggaagcg gaaaatcaac ctttttacat
    54301 attgtcgcta attatatttc taaaaacagt gggaaaatta aatataaaaa taaactgatt
    54361 actaaaaatg atgttacttt agttttgcag catccatatt ttttaggtga tttaaggata
    54421 attgataatc ttaaaattag ttcacttttt cgccatttaa acaatcgaaa aatacgacaa
    54481 aaatacacta aagcactgca aatcagccat attttgcaaa gaagagcaaa tgtatgtagt
    54541 ggcggagaaa aggcgcgtgc aaatatattg cgtggtatta ttgaacgaaa acacatttta
    54601 ttaattgatg agccaacagc ccatcttgat tttaaaaata gtcaaatagt agcgaagctt
    54661 cttagcgaat taagcgctaa taaaatcgta atagtaacaa cacaccaacc aagtttattt
    54721 aaaagtggta atactatttt tttaaggatt aagaacggag tttttcatga agataataac
    54781 attatttaaa gttattgcta atttatataa acgaaatata aagaaaaatc gattaatggt
    54841 tatttttatg atttttacta atatcacgct gatgttattt ttagttttct tttcaagtag
    54901 tgaaacacta gcccaaactc ttgcggaagg ttatttagaa tatttaagtg tggatgttgc
    54961 cctttatgaa tcgcaaccta ctaacaatcc gctgattaat gttaaatctt atcgacgacc
    55021 aagtattgat taccttaatt cgataatgaa agtaacagaa tcttttgtta ttagaccaga
    55081 ttattcaccc ttactttatg aaaaagaaat tatggtttta ggtaaagaaa ttcctaatgt
    55141 cttaattaca attattgggg agaacactaa tgagattggc attaaccgag ccttttacaa
    55201 cgaacttaat aaacttgtat caataaagga tgatgaaatg ttgctacatt tttatataaa
    55261 gacgacaata tttatcgatg atgaagaagt gttaattgat tattataatc agtttcctat
    55321 tacttatata tacgatgaac cgacatattt ttctgtccct aaattataca tttcccaaaa
    55381 tacgattgat gcaaccttag gtaatatatt tttaaagcct ggtttaacac ttaataatta
    55441 tttgcttaat ttaaatccag aacatgaact atctaactac cgctttaaat gtcactttaa
    55501 aagtgcagcg caaaaggata aatttatgca tattgttaat gaagttagca ataatgaaag
    55561 cgggattgaa gtaagtggtg atcacattaa caaaattgag agttttaatg cattgtatga
    55621 ttatttaagt atactgatga aactcttttt agcctttgtt attattggga gtgttgtcat
    55681 ttatgtggtg attgcgcata ctgcactttt aacgatatta cgacaaatgg cgctttttgc
    55741 cgttttaggc gctagtaaaa agcaaatata cgaaatatat ttatttctga ttattattaa
    55801 ttttttatta agctcggtaa gtttcttgct tttaccttct ttactacccg ttgtaagttt
    55861 cattttacat aaactcatgg gtgtttatgt tgtgcttgaa ctaaattaca cgatgttagt
    55921 gttgatgctt agcgctgatc ttctaacact acttatttta atcacaatta ttttcttgat
    55981 aaatatgcgt cgcccactat tgtatttatt aatagatgat tagtattaaa aatttagaat
    56041 gtgtactcgg tggagagcgg attcttaata tcgctagttt tgagattaat gctccggggt
    56101 tatatgtgat tcttggtcca tcaggttgcg gaaaaacaac gcttttaaat attattaccg
    56161 gtttaaatag tgaatatact ggaagtgttg agctctttaa ccgcacttat cgtcacgcta
    56221 gtaaaaagga aacgacaact tttaggtcgc gctatttaag cgcttttttt cagcacagtg
    56281 tttttatcga agaattaaca atgcgacaaa acattagtct tgtagcgctt aatcacacta
    56341 agtcgctgga gaatttagta aactctaaag tcgaaaaatt agcgaagcgt ttaaatattg
    56401 aaaaagtact taatcaaaaa gtaaaaacac tgtcaggtgg tgaaaaaact aggggcgcca
    56461 ttgctcgtac actcttaaaa gaagtcccgc tttatatttt tgatgaacca accgctgcgc
    56521 ttgatcgaga aaatgctgta atagtaatgg atttaattag ggaatactca aaaacaaaca
    56581 tcatcatttt agtaacgcat gatgaaagtt tagctaatga gtacgctgat accattttca
    56641 caatggagta tggtgaattt aaaagtgtaa atataataag aaataatgaa cttagagtcg
    56701 caaaagcatt gccggaaata ccatatgcta aaaatagtga ttacatagct aggggattat
    56761 taaaagcgaa gaaagcacga cactttttta ctggtggtgc tgttaactta ggattagtgg
    56821 gactcggctt atcatttatg ttagtaaatg cgattaatta taaactagta aatactttta
    56881 aaggggaatt tacaaatgaa acggtgtatg tacaaaagaa gttcgcacca gaaattaatg
    56941 tgcttaaaag tgtgtcacac gaagaaatag cgttaaacat ctccgcggaa aaagaattag
    57001 gctcttttta tctaaataat tttaatgatt ttttcccaca tcaaaatgag ttgtcgttta
    57061 atcacctagg cattcgccat gtccttcctt cttttcatat tggcttatta aatgaaagtt
    57121 tgtatataaa cgagatacat gagcaactct atccatatgt aacaaattta gcaattgatg
    57181 aaattggatt agtgttacca tttgatgact ttaaattaat ccaaaatgta ttacgaatac
    57241 cgatgcgtca aaccccaagt gaattaggag tgtatctaga gaataataac ttttatgtaa
    57301 cgcttgaagt gactaataat aactgggatt attttgatga acaaatattt agattacgaa
    57361 cagttacact tggcagagaa gcacaaatta ttatgggtga gcctagtttt attccattta
    57421 tttttgaaga aaaaatgatg ttcccaagtt cattaaattt aacgaaaatc gaagaatatc
    57481 cgtggacact taaaaagata aattatatgt tacttaataa tgcttttgat tacttgtggc
    57541 aatccttaaa ttactcaaat tatgtgtttt gcacggcaaa taacgactat tttcaatata
    57601 tttacaacaa aacttattta caaaataggt tacttatttt tgaaagtcct ccgcatttta
    57661 atagtatgtt caaaagttat tcttcagaga gtgaagctga attttataca tttagtaatg
    57721 gtttaacttt tattccagaa atgatgatgc ttggctttgc tcataacttt tttattacgc
    57781 aggatattac tttattagaa gcggcaatgt gggcagatat taatgatcat agtgcaccta
    57841 gttcatactt agaattacct gagggtattt taaattttgc cattcaatat aatgggtttt
    57901 ctagttttaa ttaccggaac gctaatacac cattacgaat tgatgaacta attatttctt
    57961 ctggtttagc aaataaatta gggttaagca aggacgcaca taatacggaa ttacatattg
    58021 gcgcacttgt agcgattgca gaagcaagtg aaggttattt aaaagaatac gagacaataa
    58081 aagtgtcgat taaaagggta atcgataatg atgaagttgt aatataccat cacccattat
    58141 gggcatattt actttataaa gatgtattca atgttttccc gtttgctcta gagataacgg
    58201 gcgctattta tcagagtgtc actaatgaag acttgaaaaa tttacaaaaa catttcagtg
    58261 tctcttatcc ctataaagat ttttcagtgc ttgttgccga tacattaaac gagttagagc
    58321 gttatacttt tttagcatca gttggcgctt ttcttttagc ggcccttatt acttttatga
    58381 caatttactt attagttgct gaaacaagcg accaatttag cgggctcttt ttagtgggtt
    58441 ataacgaaga agcaattcat ggggtgatta gtagatatat cctgcgcttc ctaggtgtta
    58501 ttattctttt agcaattgtg cagttattta ttttatcttt tgtcgttgaa gtagtgttga
    58561 tgaaatattt agaaactagt ttcagatatg tgtttaaccc gaaaccatat ttacttgtct
    58621 tagcgtttgc aacaggatta ctaattttct tactaactta ttttaaattt cagcatgaaa
    58681 aactcgattt tttagcgttt tcgaaacgtg atttataact ttacgacttt cttacttttt
    58741 atttcctatt ttaagcaacg aaaaattttc tttttgttat aatacaattg tgcttagaca
    58801 caaatagacg atatgtcatg gaaggattac tgatgaaagg gacgattaaa caattcaatc
    58861 aggaaagagg ttacggcttt attcgcgcag aagacggcac tgatgtcttt ttccactact
    58921 catcacttgt aatggatggc ttcaaaacca ttactgctgg tgaaaaagtc gaatttacct
    58981 tggaacaatc agatcgcgga ccacgcgcat ctaatattgt taagatataa tttaacttaa
    59041 atgagtaaat aagaggctgt tctcatgagc agccttttat tgtcactatt gcttatcgtg
    59101 actaatagta atataatact gcgggagtaa attatgggat taagtgttgg aattattggc
    59161 ttacctaatg tcggcaaatc gacattattt aatgctttaa ccgctagtaa agttgaggcc
    59221 gctaattatc cttttgcgac gattaatcca aataaaggta ctgtccgtgt acttgacccg
    59281 cgtgtcacaa aactagcgga gtattatgaa cccgaaaaaa caatatatgc aacgattgaa
    59341 tttaacgata ttgctggttt agtgcggggc gctaatcgtg gtgaaggtct tggtaaccaa
    59401 tttcttggac atattaggga atgtgatgtt attttagagg tggtgcgctg ctttagtggc
    59461 gaagaaataa ttcatgtcga acaaagtgtt gacgctaaac gcgatattga gattattaat
    59521 ttagaattaa ttcttaaaga cttagaagtt ttatatcgta ggaaagataa agttgaaaca
    59581 aaagcgcggg taaataaaga taaagattca gtgtttgaac tggagattat taataaactt
    59641 atccccgcct tagaaaacga agtgccagcg cgactagtta ctactttaac gcaagcagaa
    59701 acagattatg ttaagctcca ctttggttta ttaacgatta aaccgcttat ttatgtcgct
    59761 aatattagtg atacggaata cgcaacttat gaggataatg ttgagtatca aaaagtaaaa
    59821 gcaattgcta agcgtgaagg cgcagagctt gtggcaatca gtgctgaaat tgaagcagaa
    59881 attgcgactt tatcaagtgc tgatcaagaa ttatatttag cagaacttgg gattaatgaa
    59941 tcagggttaa ataaagtaat taatgttgca tataaaacat taggactttc ttcttttttc
    60001 acgaccggca aagatgaagt tagagcgtgg acatttaaag atggcactag tgcgcaaaag
    60061 tgtgccggta ttattcacag tgactttgaa cgcggcttta tccgtgcgga agtttattca
    60121 tataatgatt ttgaaaagta tggtagtgaa cagaaaatta aagaagctgg tagagtccgc
    60181 agtgaaggca aagactatgt aatgcgtgat ggcgacattg tctttttcaa atttaatgtg
    60241 cgaaaataaa taaaaaattg aatttttaaa taatattgat tataaataac taattatttt
    60301 tataaattgt aaaagttaat aaggtagtaa aaaaagcttt gctttacttt cttttaaaag
    60361 taaaatgaag ttatcgtagg gggaagattt ttatgaaaaa tttacaatag ctttttggct
    60421 tattttagcg tttttagctt taacatcatg tggtaaaaaa ccaagtagcg agagtccaag
    60481 tgttacaaca agcactgata caacggagcc tgaaactatc acaagtgcag gtgactcagg
    60541 aagcgaagca acaactttta caattacatg gaaaaattat aatagccaaa tactggaagt
    60601 tgataaaaat gtggagcgcg gcacgatgcc gaagtatgat ggcgctacac caacacggcc
    60661 tgatgatgat acatatacat attcgtttaa tggctgggat cctgaacctg tacctgttgt
    60721 tgctgatgca acatatgtag caaaatataa aacagcttta aaagaagttg tggatccgcg
    60781 tgatttcgtc tttgctgata caggagaagg ttacacttta tgcggatatt acggaagtgc
    60841 tagtagggta gtcattcctg aaacttacaa tgataaacca gtggtatcgc tcgaaaaatg
    60901 ggcatttttc aatcttgata atttagtgtc agtgacactt ccaaataatt tacggaagat
    60961 cggtgaagat gtgtttgccg attgtgataa tttaaaacat gttgattttg gtaatggcgt
    61021 acaagaaatt ggctttatgg catttgatca ttgtccaagt ttagaagcaa tttctttacc
    61081 agatagtgtt gagacaattt agggtgaagc attcgctaat tgtccgtcat taacaagtgt
    61141 tcggatgtca gcgaatatta aaacttttgg gttaggcgtt tttaataagt ctccgaatgt
    61201 tgaatataaa gtttatgaaa atggcttata tcttgggaat agtacactta gcgaacattt
    61261 acttcttgta gaaatcattg acaaagaaca aacaacattt aaattacatc ctgatacgcg
    61321 gactattgcg gagtttgcct gttacaaaag taatatgaca gaagtaactt catggggtaa
    61381 taatcttaag acaataggtt atggtgcctt tatgcagtct aaattaacag ccatcacatt
    61441 accaaattct ttagaaacaa ttgcagactc tgcttttaaa caaacgaaac taacaagtct
    61501 aaattttggg acaggactta aagcaattgg ttatgaggcg tttagtaatt gtcccgattt
    61561 agcggatgtt acattgccta atagtttaga acgaatagat tcaagtgttt tttatggcac
    61621 tagcgttacc tacactgatt acgaaaacgg acgatattta ggaagtggag acgccggaga
    61681 aaccgctaat ccgtattttg ctttaatgga agtaataaca aaaacgatga cgacatttac
    61741 tgttcacgct gatactcatc ttatagtgcc tggtgttttt tatggatctt ccagtcttga
    61801 aagtgtggaa attaaagaga aggtgtcatt tattggtagc ggagcatttg agaattgtat
    61861 tgcgttaacc tctcttagtg ttgccgctaa taacacgcat ttttactcgc catcgaataa
    61921 agcgatttta gaaaaggaca ccgatcgttt actattcggc ctaaatggtg gttttattcc
    61981 agaagacaca aaaatcattg gtgaaaaagc attttattac cggaaattta ctgagccttt
    62041 agtcattcca agtagtgtca cgacaattga agattatgct ttttatcaaa ctaatttaac
    62101 agcgattgtc attcctgata gtgtcactaa aatcggaaaa tcagtgttcc aatatagtaa
    62161 tagcctaact tcagtaacct taagtaagaa cttaaaagtc attgaggact ggacttttag
    62221 tggttgtaaa atactagaaa cacttacgat acccgactca gtcacaatga taaaagagtg
    62281 ggcatttagt aattgtgaaa tgcttgctaa tgttacttac agcgctaatt tagcgtttat
    62341 tggtgacgtt gctttttcaa gttgcgttgc cttaactacc cttccagcat ctgaaagttt
    62401 gcgagtgttt ggcagtgggt catttagagg gtgtgagagt ttaacaagta ttacgattcc
    62461 taaaaatgta cgctatattt ctagcggcgc ttttgctgat tgtacggcgc taacaacatt
    62521 aaatgttgta agtgaaaata atcattacga ctcacgcgaa aattgtaatg gtattatcga
    62581 aacggccaca aatacattaa tggtcgccgt taaagatacg gtgattgtcg aaagtattga
    62641 agctttagga gcagaagtat tttcaggcat tgatattcaa agtattacga ttcccgcaag
    62701 tatcactaaa atcggtaatt gggcatttag tgactgtgga aagctagaaa cagtcgtaat
    62761 tgaaggtgaa cttacgatga taaatgctgg tacattttca cattgtactt ccttaacgag
    62821 tgttaatatt ccggaaacag tgactgaaat tggtggcagt gccttttata attgtaaagt
    62881 tttaccagag attaaactgc ccgctaattt ggagaaaatt ggtagttggt gttttggtcg
    62941 ttgtaccatt ttaccgcaaa ttgtcattcc tagaagtgta acaaatattg ctcgttctgc
    63001 ctttagcagt tgtgcgaatc taacaattta tgccgaagca acagcactac cagatggatg
    63061 gcacgctgat tggaatgatg gtaatcgtcc tgtttattgg tatagtgaaa ccgctaatta
    63121 cgatggtagt cactggcgtt atgttgacgg agtgcctaca gtgtgggcag aataatagac
    63181 tctagttatt atttataaaa gaaaggtggt tgttgtttgt tttatagcaa taatcacctt
    63241 ttttgcttgt gaaaagtgga tttactttat aatatacttt aaataaagta tacttaatcg
    63301 ttgaggtaag aaatgattga tatccgatta caagaattaa ttaaagatgc aactgtgaaa
    63361 ctgggactag atatagacca agacacaatt gtgattgaaa cgagtaaaga tagtgctcat
    63421 ggggattacg ctacaaatat tgcgatgcaa ctagcgcgtc cgttacgcaa gaatccgcgt
    63481 gaagtagcga ctttactcat taatgcgatg gatcaaacgc catttgaaaa aattgaaatt
    63541 gctggtcctg gttttattaa tttcttttta aaaccagaaa cattatcaaa atatattgcg
    63601 actattttaa aagaaggcag taattttggt aatggtagta aaaaagatac gaaagttaac
    63661 attgaatttg ttagcgctaa tccgactggt aaacttcatt taggacatgc gcgcggagcc
    63721 gcagttggtg atacattagc aaggctcttt attaaaagtg gctacgaagt tatccgtgaa
    63781 ttttatgtta atgacgctgg tgtacaaatt gataatttag gtaaatcact ttatgtacga
    63841 tacttaaatt tatttggcat tgaaaaagag ttaccaaaag acgcttatca tagtacggac
    63901 ttaatcgcca tcgcggaaag tttaaaagct gaaattggtg ataaatatgt taaagagagt
    63961 aaagagggtc ttgaatactt taagcttgaa ggtacaaaca aatttttaga tattattaaa
    64021 aaagatttac aaaactttga tgttgtcttt gatgtttata gctacgaaac aaaagtaaga
    64081 gcaggaaata cgattccaaa gttactagaa aaattagcgc cttttactta tgaagaagac
    64141 ggggcaacat tcttaaagac aagtgacttt ggtgatgata aagatcgtgt cattattaaa
    64201 agcaatggtg actttactta tttcttaccc gacattgctt atcatttaaa taagttgagt
    64261 cgtggctcat cacttttaat tgatgtctta ggtgctgacc atcacggtta tattggtcgc
    64321 atgaaagcag cgttacaaat gtttaactat cccgctgata cacttgaagt gcaaatcatt
    64381 caactagtgc ggattattca agacggaaaa gagttaaaga tgtctaaacg gacaggtaat
    64441 gcgattactt tagaagaatt gtgcgaagaa gttggggttg atgcagcgcg gtactttttc
    64501 cttgctaaat cagcgtccac ccactttgac tttgacctag atttagcaac tgaaagttct
    64561 agtgcaaatc cagtttatta cgctcaatat gcacacgcta gactcagcgc cgtcttagcg
    64621 cgcggtgaaa agtttagtct agacccaagt ggggaaggtt taaaagaagc tgctgaactt
    64681 aacctaatta agcaattaat tgcttaccca agtgctgtta gtgatgctac aatgactaga
    64741 gagccatata aaatcgctaa ctatattcaa aaactagcga gtttagtgca ctctttctac
    64801 actgaagttc gtattttaga tgaaagtaat gtgcaactta ctagtgcacg gttaggctta
    64861 tcaatagccg cacaaattgt attaaaagat gctttaacac ttttaggtgt tagtgcaccc
    64921 caaacgatgt aaaatataaa ggagattaac tattcttaat caagttaaat agtaaatata
    64981 tatcaaaaag a
//
LOCUS       DAOSTA010000003        46742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_880_length_46742_cov_8.091889, whole genome shotgun sequence.
ACCESSION   DAOSTA010000003 DAOSTA010000000
VERSION     DAOSTA010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 46742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46742
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_880_length_46742_cov_8.091889"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..656
                     /locus_tag="PLU08_00620"
     CDS             117..656
                     /locus_tag="PLU08_00620"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOQ70246.1"
                     /translation="MYENERFLRISDTASGFTDHRLKKQYFALFDWYKRNLEYEMFLA
                     NNKLRYKINQGEVYEIDFGRNVGSELNERHYAVVLHHSDVEAQNIVVVPLTTKIHFSY
                     GEAIDLGYLPEVKTTEKSYAKISQIRTVDKARIYLRPIIHTEDNKPCRHTYGPVTKLT
                     ADQFRLVIEGLNKLLNNQL"
     gene            complement(894..1976)
                     /gene="nadD"
                     /locus_tag="PLU08_00625"
     CDS             complement(894..1976)
                     /gene="nadD"
                     /locus_tag="PLU08_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOQ70247.1"
                     /translation="MKIIVYPGSFDPLHNGHLLIARHAKREIGADLVLFLLSPSTVWK
                     KVQTSFNHRANMISAALKDEEGFELSRIEEGNEGKLNYTYLTFMRLREHYPHDELYLL
                     LGEDQAMAFDQWKHPELIAEQAKILVYKRKGSKVSNENFERFDMLEITGPLSSTSSTK
                     VRNFESIDVPSVVLEYIGNNKLYFSGLVAKRMSDERYYHSLSVASLSRLIAIANDVDP
                     LKAFKAGLFHDIGKEVPAAKSLKIMEEHFPKYLNMPLWSHHQFVGAYLMKEEYLEKDK
                     KLIDAVKYHATGHKKMSKLAKVIYAADKIEPSRGFDSSQYIAECVKDINKGFVIVLQA
                     NKDYLISKGKDIDNALTNACFKKYLK"
     gene            complement(1973..2266)
                     /locus_tag="PLU08_00630"
     CDS             complement(1973..2266)
                     /locus_tag="PLU08_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HOQ70248.1"
                     /translation="MLTKKEMRYLRGLSNKLKAQYQLGKNEITDSFLSLLEKALVAKE
                     IIKVHVLQSVDKEVTSLALEVAARLNATVVETKGKTFTLYRQNVKAPKIKFPL"
     gene            complement(2271..3398)
                     /gene="yqeH"
                     /locus_tag="PLU08_00635"
     CDS             complement(2271..3398)
                     /gene="yqeH"
                     /locus_tag="PLU08_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HOQ70249.1"
                     /translation="MTENLKKIKKCYGCGVVLQSENEEEIGYVPSNVLKRDDVFLCQR
                     CFKTHHYSADKLHSPVVTLDFMNLIKDIKAKNALVVYVVDVFNFESSFNPEINTALSG
                     LDIILVANKFDLLPKSTNEAKLKDYVATKVEREGLNVKKIIVASSTRNYNIDEVLEEM
                     ANFRKKGDVYFIGAVSSGKSSLIKTLLRNFKNETSRYITTSIYPNTTLAVQQIPISET
                     TTIYDTPGIAINNSLLGLAEKEVVKVIVPRKEIKPRTYQLLAKQSILIGGIARFDYVD
                     GPFTGFTFYFAEGIKLTRTHIKLADRTFNNMIAHQKTRPISYLVTGVQDLDAFEFTTY
                     ENKKYDVSWNGLGFVSFKGSGRVVRIYAPRGVAISYNESKI"
     gene            complement(3373..3912)
                     /locus_tag="PLU08_00640"
     CDS             complement(3373..3912)
                     /locus_tag="PLU08_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HOQ70250.1"
                     /translation="MKKFTPNYYAKNIYEVSADFYLKHGIKTLLVDLDNTLASYRELE
                     PSDTTINFVRNLEKAGIKVLLASNNSGKRVKRYGELLGVPYISGARKPFAFKLRKFLK
                     EQGAELSSTLMVGDQILTDVFCGNTLGVKIMLTDKLVEEDQWTTRINRLLDRPLRNKL
                     RAKGKLKEWNEDDRELKEN"
     gene            4058..4327
                     /locus_tag="PLU08_00645"
     CDS             4058..4327
                     /locus_tag="PLU08_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70251.1"
                     /translation="MNLSKLKPYRNMLFLALAAGVGAFMPVIGIIVTVVMYVKRDENS
                     LNFTKEERFLLNALLIILIIYLTLNVLYTLKYPEVKPDTSSETSL"
     gene            complement(4324..6912)
                     /gene="parC"
                     /locus_tag="PLU08_00650"
     CDS             complement(4324..6912)
                     /gene="parC"
                     /locus_tag="PLU08_00650"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOQ70252.1"
                     /translation="MSKRKPKKQTEEQVITETIIELPMDEIMGDRFGNYSKYVIQNRA
                     IPDARDGLKPVQRRIIYAMHDEGNTFNKPMKKCAHTVGAVMGKYHPHGDTSIYDALAR
                     MSQEWSMRYPLIDFQGNNGSIDGDEPAAYRYTESRLNELSNELVLDINKKTVNMNLTF
                     DDTSFEPEVLPSRFPNLLVNGSQGIAVGVATEIPPHNLREVVDAIIHRITYKRSTIED
                     MRKYILGPDFPTGGVIYKGEGLDSIYETGRGRVEMEALATIESDRTSQRIVITEVPYQ
                     SNKSQILRELGAIEQNRNVDGVVEVRDESDRNGIRIVIELKKEANTDVILEYIFSKTR
                     LRAGYNANIVAIADNRPKTLNILDLIDAYISHQVDVITRRSEFDLATNEKRLHIIEGL
                     IKAISVVNEIIAIIRKSTDKANAKTNIKAAYGFSEEQAEAIVMLQLYKLSNTDITVLM
                     EEKAAIETTIDELRNILSNEKALNNVIIRDLKRIAQKFGDDRRTKIREKGEVVQIDKR
                     DLIADEQVMLALTRDGYIKRSSLKSYRSSGDFALPGVKSSDLLIAASEVNTKDFLVAF
                     TDLGNYLYIPIYEIGEGRWKDEGKHINYIVQLNANEKIIKAFNVKEFREDLYFAIATR
                     NGMIKRTSLNEFVAVRYSRPLQAMRLLSGDRVADVTLTTGNSELLVITESGAASFYNE
                     NEITVVSVKASGVKSINKKKADKVVALISVDPEVRTRTCIVTSEGHLRLLDHSYFEAT
                     ARLGRLQHVMRTFKSDPHTIVSAFRYNRNIEELRVYALTDANVILPLLLTDLAPTPVD
                     KYAKRNINELAEEEKIVSIFNEEIPTISEETKAYAPIVKAEAVKVEPVEDSYEQISIF
                     DLLDED"
     gene            complement(6914..8881)
                     /locus_tag="PLU08_00655"
     CDS             complement(6914..8881)
                     /locus_tag="PLU08_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HOQ70253.1"
                     /translation="MADKNITNSYTADSIQVLEGLDAVRKRPGMYIGSTNIVGFHQLV
                     WEVVNNSTDEAMSGYGDKITVTIHKNGSITIADEGRGIPTGINKQTGQPAVHLVFSTL
                     HAGGKFSESTYKTSAGLHGVGASVTNALSEWLDVTVYREGKVHHLRYENGGKLVTPLE
                     VVGNTNRTGTIVNFKPDPKIFGNLEFKWDTITSHLQESAFLLNKVAFIVKDERSGLSE
                     EFYYEKGLIEYVDIINRNKTALSKPIYFEDIYTNNDKLSQPMEIEVALQYCSDDYGET
                     VLSYVNNLRTHDGGTHETGFRTGLTRAVNDFATEHNLLRKGVKLDGNDIREGLTAIIS
                     VRIPESILQFESQTKGKLGTNEANWALNSFIYTKLTYYLQENHEFAVSLIKKCVDAQN
                     ARIAARKAKENLRKTKKVTNNDMILSGKLTPAQSKDYKKNELFIVEGNSAGGTAKSGR
                     DRMYQAVLPLRGKPLNTDTSTVDKMLKNEEFATLISTIGAGFGQNFDIEDAHYGKIII
                     MTDADYDGSHIQTLLLTFFYHYMKPLLKAGMIYIAVPPLYRVSKQVNKKLIFEYAWDD
                     QSLEAAKEKIGPGYKITRYKGLGEMNADQLWETTLNPETRTLIRVNINDPLLVEMRVS
                     TLMGNDANVRRKWIEENVDFNAYDTFIDEVK"
     gene            8986..9669
                     /gene="plsY"
                     /locus_tag="PLU08_00660"
     CDS             8986..9669
                     /gene="plsY"
                     /locus_tag="PLU08_00660"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOQ70254.1"
                     /translation="MHPFIKFLIFLGFGLFGYLIGSINNAVIISNLFFKRDVRNYGSK
                     NAGGTNTGRVFGRKAGITVMVLDILKSVFVYWAITLVFHFTNVGLYVDHAATLYFAML
                     LVALGHCYPVYYKFKGGKAVSVVAGFILATNWVFTILGALIFFLILKLRKMVSYASIL
                     MAAIIVALTPILFIPVLGRISFYPLVVDNLYYYIPTLVGLCILLILKHRTNIERLQKG
                     TESKITWIK"
     gene            complement(9739..9951)
                     /locus_tag="PLU08_00665"
     CDS             complement(9739..9951)
                     /locus_tag="PLU08_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016340731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HOQ70255.1"
                     /translation="MPIGAAIGLAVFALILGAVLGYLISRKVFEKQLKKNPPITEKQI
                     RTMLTMMGRKPSEKQVREIMRGMQQS"
     gene            complement(9951..10508)
                     /gene="efp"
                     /locus_tag="PLU08_00670"
     CDS             complement(9951..10508)
                     /gene="efp"
                     /locus_tag="PLU08_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOQ70256.1"
                     /translation="MINVTELRPGNYFTEAGEIYNVLDILLNKTAMRKMIAKLKVKNV
                     RTGAITEQTYNSGYQIDRISISKHKMTYLYDSGDFLVFMNTETFEQVELSKESLTWEL
                     NFIVPNSEVEMAGYEGEIFGITLAPKVALKITECAPGVKGNTVQNATKDAIVETGLRV
                     SVPLFIEEGEVIMVKTTDGSYDGRA"
     gene            complement(10542..10691)
                     /gene="rpmG"
                     /locus_tag="PLU08_00675"
     CDS             complement(10542..10691)
                     /gene="rpmG"
                     /locus_tag="PLU08_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOQ70257.1"
                     /translation="MRVNIQLRCTECNEENYLSSKNRKNNPERIEKMKFCPRCNKQTL
                     HREKR"
     gene            complement(10769..11662)
                     /locus_tag="PLU08_00680"
     CDS             complement(10769..11662)
                     /locus_tag="PLU08_00680"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015009864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOQ70258.1"
                     /translation="MSDKLILGSHVGMSAPDYYIGAINEALSYGANTFMIYTGAPQNT
                     RRLATEQMHALEGIKYLKEKGMDIKHIVAHAPYIINLGNSVKPETYELAVSFLQDELQ
                     RVADFGITMVVLHPGSHVGAGVEAGTEQIIKGLNTVLDNDTSGTIIALETMAGKGTEI
                     GRTFEEIRTIIDGVHKKSQLGVCLDTCHINDAGYDVHDIDGVLDEFDRVIGLEYLKVV
                     HVNDSKNERGAAKDRHENIGFGTIGYDTLMKYIYSPRLAHLPKILETPYINGVAPYGH
                     EITMIKNKVWNTTLKDELGEK"
     gene            complement(11659..13014)
                     /locus_tag="PLU08_00685"
     CDS             complement(11659..13014)
                     /locus_tag="PLU08_00685"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOQ70259.1"
                     /translation="MEFGKYNIDKRLVDTLKMHGYVTMTGVQSEILPRALKGESLIVK
                     AKTGSGKTHSYLIPILNKLEKTEELQALILAPTRELARQIYDFARVLNDDYKGVDILF
                     LAGGLQTSRHKERLASKPALIIATPGRLKALLINEDITSFKSIHTVVLDEGDMLLDSG
                     FYDVISETLAALDNPTVQLFSATIPQKLANLVTLKTGVKTVIDIDRDDKTSDNVTHHL
                     IDVHHMNRFEVVESFVKHFNPYLLLVFCSTNKEVLELYEFLSAKGHKIGLLTGELESR
                     KRKAMFRRVRNNEFQVVVASDIAARGIDIEHVSDVLSVSFPFDLEFYFHRAGRTGRLD
                     NKGNAYTLYDHDDLETVEKVEKLGVKFTRLVYKGGAFSAVTKRISRKKALTPEEKELN
                     VKIKTAVAETKTKKVRPGYKKKVRTAVENVKRKHRRKMIKKSIREQRVERYKAEKRRD
                     S"
     gene            complement(13079..13852)
                     /locus_tag="PLU08_00690"
     CDS             complement(13079..13852)
                     /locus_tag="PLU08_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOQ70260.1"
                     /translation="MKTRTLLTKLGRLYPKRIALKHNDYVGYMAGQKVEEVKRIVLLL
                     DLDEIVLEEALSHKPDLILTHHPFIRGTRAKVFARDEKKKAFALWLDTLPVTVYSMHT
                     NFDEAPNGMNDALAEKLELSAVKPLASEPMARGGYLPYPMTVEEAANYIVDKLEATYG
                     LLVAGDKEREIKRVAIIGGGGSRDWPAAMAEGYDMYISGDAPHYVRRDVMNAGYNYLD
                     VPHEIENAFLWKMKEVLLTIDPSFEVIAIKHEKPPVVIK"
     gene            complement(13849..14517)
                     /locus_tag="PLU08_00695"
     CDS             complement(13849..14517)
                     /locus_tag="PLU08_00695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ70261.1"
                     /translation="MLSLRLQAIANLIKKDDVVADIGSDHGLLVNYLAIKGHRALYAS
                     ENKKGPYNRLKSELKAYKSDIIEIDLADGLENLPARINTVVIAGMGGELIVSILQAHP
                     AKLVNVQKLILAPHCNAKAVREVLNALNYSISYEEIVVEKEVFYEIIVAEQKAIATKE
                     VNPYFGTYHLQARSENFIKMWTSVYNQNKRILSNKKLPLSRKEELLKEQELIEKVISE
                     GEKV"
     gene            complement(14517..15968)
                     /locus_tag="PLU08_00700"
     CDS             complement(14517..15968)
                     /locus_tag="PLU08_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOQ70262.1"
                     /translation="MDDKKLKDLAELEEVAIEEDDEVEIELTLPQIRKFLLEEARRNN
                     NELDQDDVMDATSHLDLSENEFDKLIQYFKNNKVTIHSDIESDEYDPDFANFVDGDDD
                     LDDDLDLDDDFDEEELSFGDAFKDVKEENFEENQRLRKISLGEVKTTDSVKIYMKDMG
                     RYSLLKADEEVTLARRYKEEGDIDARDELITRNLRLVFNEAKKYSSGNMHILDLIQEG
                     NIGLMKAVEKFDYTKGFKFSTYATWWIKQSISRSIADQARTIRIPVHMVETINKISRT
                     QRRLTQEFGRDATPEEISDALIKYSVKDVEEAMELSANATEENPIPEEVANKVRTIMA
                     KVETLTEELKRPATPEEVSESFREFTPARIREIQAIAMEPVSLESPIGEEDDSHLGDF
                     VEDKDNETPTDFVRKSILHDQLRDNMQEFLTPREERVLHLRYGFDDDKPRTLEEVGKI
                     FGVTRERIRQIEAKAIKKLRHPSRTKGLRGHIQ"
     gene            complement(15987..17768)
                     /gene="dnaG"
                     /locus_tag="PLU08_00705"
     CDS             complement(15987..17768)
                     /gene="dnaG"
                     /locus_tag="PLU08_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOQ70263.1"
                     /translation="MENVDIVDVIGRYIPLHKQGQNYSALCPFHDDRNPSLMISPSKQ
                     IFNCFVCNTGGNAIRFVALYEKKTYYEAAQRLADMVSFKHDFFTKKQKFVVRDTKLDP
                     LISAINDLTAYYEYTLITEAGSEAKKYLVNRGLDDALLKKYKIGFAPRDGEATIKFLQ
                     SKGHALKTIEDIGILGGSLERPYDINHGRIIFPLNNKDGQVVAYSARALPSEKKAPKY
                     VNTRETQLFIKSDTLYNYHHAKALMAAQYVYLVEGFLDVIALDRVGERSVVALMGTAF
                     TKEQLALLRLLNKEVRIYLDSDTAGQMATLSVIKDLIAANIPLQIVKPHKDGYDPDDI
                     LNKEGKEALVTALTTFIPRDDFIFNYYLSVTKLDDMASTKKFVNDVMNEIILKLNSRL
                     EVSAMIERLAEASGFNSLVLTDMYESLRRKKQTRNEAINTVKQKLPVRQTLNKVAQAE
                     RFIVYQMLVYPEARTFFDNHVRVFTHEVHRYIVNYLKEFKPKEQVNYALLLNDIQSRF
                     TDEATINEYTQELLTIESGLQDVKYDENILNDAAKTLNYERELQILERKHQDELLRAP
                     DEASYAALKVKHIQAKTELMQKYGKKK"
     gene            complement(17830..19203)
                     /locus_tag="PLU08_00710"
     CDS             complement(17830..19203)
                     /locus_tag="PLU08_00710"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOQ70264.1"
                     /translation="MHKHSFEKIVNHLVTSGFVFQGSEIYGGLSNTWDYGPLGTSVKN
                     NISNLWWKKFVTESPYNVGIDAAILMNPKVWHATGHVTTFNDPLIDCRNCKARHRADN
                     LIEDQVKGLNVTGLPNEEMMKIIKEYGVKCTVCGKQDFTDIRQFNMMFKTHIGVTEDS
                     QSEVYLRPETAQGMFVNFKNVQRTMRKKLPFGIAGIGKAFRNEITPGTFTFRTREFTQ
                     MELEFFCKPGTDLEWFKYWKDYCIKFVQDLGIRPEYLRFRDHTKDELSFYSLATTDIE
                     YLFPFGWGEIWGIADRTDYDLKQHMEHSGENFEYLDPDTNERYIPYCVEPSVGLDRLL
                     LMTLVDSYDEEELEKDTRIVMRLAPHLAPYQVAVLPLQRKLSEKALAVFSDLVKDFST
                     VYDETASIGKRYRRQDAIGTPYCVTIDFDTLEDESVTIRERDSMAQIRLPLSELKAYL
                     HSKVKFQ"
     gene            complement(19276..19983)
                     /gene="recO"
                     /locus_tag="PLU08_00715"
     CDS             complement(19276..19983)
                     /gene="recO"
                     /locus_tag="PLU08_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOQ70265.1"
                     /translation="MEVVIIRLTPYREKDYIVNALSKEEGIITFRATGALNVKSKFAG
                     KLFLYALVDVELRESKAGHTLTNVESLTNIAKVFTDEKRIIALNIIGEVISKTLKDND
                     SISEAFPLIKKVLMSLSETTKLISLVYLFTTNLLRILGSALVIDRCVNCGDTKSIIGV
                     SFNKGGLICKKCYDEKTITLNAEEIQMLRYGFMINEEELLRHEFSDEAALSLLTLMLT
                     YLEFTYNYKIMSKDLIK"
     gene            complement(19986..20858)
                     /gene="era"
                     /locus_tag="PLU08_00720"
     CDS             complement(19986..20858)
                     /gene="era"
                     /locus_tag="PLU08_00720"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOQ70266.1"
                     /translation="MIKSGFVSIAGRPNAGKSTLLNTILGEKRAIVTPKPQTTNKNIQ
                     GVYNDLRGQIVFIDTPGIHKPKTPYGEYLNKQAYQSIRGSEVTLLVVDASVEYGAGDE
                     YLFSRLKVDHPLIIVLTKIDLASPIHVQKVKEQWTQNYPDTPLIEVSSVRNFNIDALI
                     TLIFEHLEVGPLYFPLPEDYAIRLENYINEIVREKIIMLTREEIPYSVAVVVDHLTES
                     DTKVEAYLTIYCEKDSQKGILIGKGAKMIKRIRLQTQYELSKFLGKDVLLELMVKVEP
                     LWRSDPKILKKLLG"
     gene            complement(20855..21253)
                     /gene="cdd"
                     /locus_tag="PLU08_00725"
     CDS             complement(20855..21253)
                     /gene="cdd"
                     /locus_tag="PLU08_00725"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOQ70267.1"
                     /translation="MTKEELIKKAREARALAYVPYSKFKVGAALLTKSGKVYVGANVE
                     NASYGLCNCAERNALFHAMMNGEKKEDFVALAVTGKTAGPISPCGACRQVMSELLPKD
                     APIYLTNLEDAFLETTIAELLPFAFDDSDL"
     gene            complement(21250..21720)
                     /gene="ybeY"
                     /locus_tag="PLU08_00730"
     CDS             complement(21250..21720)
                     /gene="ybeY"
                     /locus_tag="PLU08_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOQ70268.1"
                     /translation="MMLKLTVEFYDDYAPTYKNYRAIFKRIYAKTMKLLDYMTGEFLV
                     EVTITDEERIRALNKEYRAKDAATDVLSFAFNDIKPGHKFKEPFVHLGSIIICVPVAQ
                     RQALELGHDARREFKFLFTHGLLHLLGYDHTNEKEEQVMITLQDQIIGKRGGKK"
     gene            21890..22525
                     /locus_tag="PLU08_00735"
     CDS             21890..22525
                     /locus_tag="PLU08_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HOQ70269.1"
                     /translation="MKSDFTFIYSAPKVTDEKLPAVFLFHGYRSDEKDLLSLVASLES
                     THHIFSVRGPLSFMFGGYAFWDMQDLENPNLEQYQNAVESVQNFIVNAITQYNLEKKD
                     ITLLGFSQGAVLVQSIALRAQKLVNKVVALSGHLPYFAKDEEYATLNLNKLHIFISNG
                     INDNVVPYAWGKGSADYFKKRGADVTFKSYFSGHYVSRDNARDLIDFIVSN"
     gene            complement(22544..23254)
                     /locus_tag="PLU08_00740"
     CDS             complement(22544..23254)
                     /locus_tag="PLU08_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70270.1"
                     /translation="MFLIFLLIGFAFLGHASLDLISYVSFTLAAILKIIEQQVIMKNL
                     QKFDDLYIAPRLVNIKDNFKYKFKVLQEAKTVVFSRPLQLFSAISSTLLITIILVLNN
                     YYFVIISVLLMIVALIEVKRGIKGSAKTFELEVKLSSLAKENNEKRMQLYKEIINDST
                     HIAAWRVYRSIIIHFSLGVLIFVLMYFTAVYSLNFLIFYFFGFSYYLYELKKLFSLAL
                     NTHHYYSAINAIYDIPVN"
     gene            complement(23457..23711)
                     /locus_tag="PLU08_00745"
     CDS             complement(23457..23711)
                     /locus_tag="PLU08_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HOQ70271.1"
                     /translation="MNLIDVRLTQNNTNECGKAVLRMLLAITHRDKRYLKLPLSGDFD
                     NFLALSKKAKEYGVELLGYKLDAIEEVKKVKGPFVMHLSR"
     gene            complement(23793..23972)
                     /gene="rpsU"
                     /locus_tag="PLU08_00750"
     CDS             complement(23793..23972)
                     /gene="rpsU"
                     /locus_tag="PLU08_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOQ70272.1"
                     /translation="MPKTVVRENETLDEALRRFKRQVNKAGVLSETRKRQYYVKPGLR
                     RKLKAEQARRKARRG"
     gene            complement(24694..25290)
                     /locus_tag="PLU08_00755"
     CDS             complement(24694..25290)
                     /locus_tag="PLU08_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70273.1"
                     /translation="MSNKKDYEDIIKGIGFMKKYKRNIMIKVAGVISELIITFIPIYL
                     ISSLLLFPPLIFKDFIKFTGLTILVIVIVNIIFYIIAAIAKIFIKTAYIITSDSLITQ
                     INNEVHSTIYLKDVYYLTYNFGAIDGQPSPTELVLFDKEHKYLISIENPPLLMVINIK
                     KKCLNAKITYFNSRRILFFLVSSLVISIIVSLISLIAN"
     gene            complement(25471..25749)
                     /locus_tag="PLU08_00760"
     CDS             complement(25471..25749)
                     /locus_tag="PLU08_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70274.1"
                     /translation="MKKRIIAFYDVLIVAIISTPFTICSIFFFVFADTSNLEWLCKNW
                     YLIIFASLGVSLPVVGIFWITGYTLIMNNHYVFFYYFPHAKTWKKNDK"
     gene            complement(26322..26477)
                     /locus_tag="PLU08_00765"
     CDS             complement(26322..26477)
                     /locus_tag="PLU08_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70275.1"
                     /translation="MKIYTINDLKLKYKYSEKNIKKRHYKLLLNLLKSVNSGDYDINR
                     YYSLIRN"
     gene            complement(26602..27003)
                     /locus_tag="PLU08_00770"
     CDS             complement(26602..27003)
                     /locus_tag="PLU08_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70276.1"
                     /translation="MSDTAEFVKVEVIDEENKEGMSRRKKFEIRKLVMSTYVTLVVIA
                     YLAMVFFLDKWHPHWVIFLSIPVVDSLVTAILEKKIALFSVDALIILAFVVVGAVTGK
                     WHPAWMILLLVPLWRYILNTIKRIKRIKAMD"
     gene            complement(27022..27462)
                     /locus_tag="PLU08_00775"
     CDS             complement(27022..27462)
                     /locus_tag="PLU08_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70277.1"
                     /translation="MQFNQDFDKIIGEDIYLSITEKNSGLIGGLPYYKYGIHLNDGTL
                     VGNILVRIGHNFNSYYSGNFVYQVSGAYRGQNLAVKALNAILVVAKYHKMEYVIFSAP
                     SSSKGYLRTFEKMGAKLLEIVTPPENYMYYYKGIEPLAIYRLDL"
     gene            complement(27500..27985)
                     /locus_tag="PLU08_00780"
     CDS             complement(27500..27985)
                     /locus_tag="PLU08_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70278.1"
                     /translation="MITFEKSKRYLFYYVGVLLVTITLFISWVVMIVKNPGSTSGDIV
                     LLSFLFLILFGGEALFLIIYLIRPKVVLSVDEEGAYLHLSKKKTKEVAFSNFSSVGNM
                     RRNLVIATKDSELYVVRFLKDHKKAEETLKTMFNTFITNHPERYFDMTESADKQKHEE
                     K"
     gene            complement(28000..28662)
                     /gene="deoC"
                     /locus_tag="PLU08_00785"
     CDS             complement(28000..28662)
                     /gene="deoC"
                     /locus_tag="PLU08_00785"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006267712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOQ70279.1"
                     /translation="MKKNKLIDHTLLKADASPAQIEKLCQEALEYDFMSVCVNPGYVP
                     LASMLLAESDVRVCTVVGFPLGATSTEAKVLEAQIAVEEGADEVDMVINVGKLKAGLD
                     LEVFTEIRAIREALPDWVILKVIIETCLLTDDEKVRACLLSRKAGADFVKTSTGFSTG
                     GATVADVKLMRDTVGSDMGVKASGGVRTMEDLEAMVKAGANRIGTSNGVAFFTGKKGT
                     GY"
     gene            complement(28882..29085)
                     /gene="rpmF"
                     /locus_tag="PLU08_00790"
     CDS             complement(28882..29085)
                     /gene="rpmF"
                     /locus_tag="PLU08_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOQ70280.1"
                     /translation="MAVQQRRGSKTRKRLRRTHFKLNVTGLTTCPNCGTLIRAHHVCP
                     ECHHYAGKDVITKEEPKKGKAKK"
     gene            complement(29224..30510)
                     /gene="mtaB"
                     /locus_tag="PLU08_00795"
     CDS             complement(29224..30510)
                     /gene="mtaB"
                     /locus_tag="PLU08_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOQ70281.1"
                     /translation="MKTFTSVALGCKVNAYEVFSIEETLKAQGYKLAHQGDKVDVAII
                     NTCSVTSNADQKSRQHIRKLAKNYPDAILVVMGCYAQLNKDLINTIPEIDILVGTSNR
                     SKIVDYIEEFKEHGQKIIAVEEEPRTFNYEELGATSVTPQARAYLKIGDGCDAFCTYC
                     IIPLARGKMRSRDVRDIVKEAKDLVRRGYKEIVLTGIHTGAYGKDLGNIKFSNIVEMI
                     LEACPSLHKLRVSSIEANEIDDDFLRLMLKDKRIARHLHIPLQSGSDEVLRRMNRHYT
                     TSEFIHKIENIREKVPNIAITTDVIVGFPGESEKLFNETVAFIKKVNFTQLHVFPYSA
                     RTRTPAASFKEQVLGPVKKERVRTLINLSEDLYRSYAQRFVGEKMQVLIETYDDKEEV
                     YVGHTTNYLAVRVKSDSDIKHEVITIIYDPNISKKI"
     gene            complement(30507..31214)
                     /locus_tag="PLU08_00800"
     CDS             complement(30507..31214)
                     /locus_tag="PLU08_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70282.1"
                     /translation="MKIYELLQENRRGHLITPSREYLFFQDHEALLTQVPELIPFINS
                     KDDFDLICANIIQSALLNYEALTNYWNNEVKPDTELPVKALFTYNNVPIYCPLFSVSN
                     NILIGNNLGSKLNLTHDAIDLFEAYNFSLFESQLTSLVLVGEDIHTRAYYHYDFHAIY
                     IINDQGRLDLKICLFDKHIKRPDFRNVLERIKPALTEYYAGNRIAFINALVKEKLISH
                     KLYTKYINNLKRRKIIG"
     gene            complement(31201..31926)
                     /locus_tag="PLU08_00805"
     CDS             complement(31201..31926)
                     /locus_tag="PLU08_00805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOQ70283.1"
                     /translation="MSYRFFGRVRNNEVVLSDDDIFHLTKVLRLKKDETFEVVAGQSL
                     YLCTYESVNPFILNVLEKRKLPLEPLPRLTLGYALPKGDKLELVIQKAVEIGVSHIIL
                     IDSERSIGKIPATRLESRLARFNKIIKSAAMQANRNYLPTLTEPLAFKEVLDLEFDTK
                     LIAHETANLPLKAVLQQDLGDNLLILVGPEGGFSEAEVTLASTKGFNVITFGDNILRS
                     ETAAIYALSVLHHYQKGAHHENL"
     gene            complement(31954..32769)
                     /locus_tag="PLU08_00810"
     CDS             complement(31954..32769)
                     /locus_tag="PLU08_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOQ70284.1"
                     /translation="MEILKYIILGIIQGVTEIFPISSSGHLVIFQELLKLEQPGLVFE
                     MFTNTASFLALFVLFAKDIWELIKGFFLYIFKKEAREEYKDTFFYVLKLLVAVIPLGV
                     VGLIFKDEMGMIKNLLTVGIALFITGALLLTIFFTRNLIEEHDDITWKDAIVVGFTQA
                     FAVFPGISRSGSTIIGGRASRVSLKSILRFSFLAYIIISVPTSVLGAVDLVHISEEIN
                     WTGYILAFIFTFGATFLTGLFVMKKLKVEHLIYFGIYCLVVGILAFSAFFIMR"
     gene            complement(32885..34516)
                     /locus_tag="PLU08_00815"
     CDS             complement(32885..34516)
                     /locus_tag="PLU08_00815"
                     /inference="COORDINATES: protein motif:HMM:NF040368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6273 domain-containing protein"
                     /protein_id="HOQ70285.1"
                     /translation="MLSKATLAFFALSALLISAISLGSNHGSSARGEPTPYAIVLGTE
                     KNKIAEGSENPDGYTGTGVLTTELGNNIAVSYKHLTNPSTHWQRIKTGGYIVNSDPIN
                     GITNLELTKRDDTVNVEILYSNTMSFDASRKVVINASSPTNFSTDFNGYKPKYIGIYA
                     LADSSFNLGKIEFDCVDHYHTLSVLSKNDARGTVTSEGLFRAGTNVTVTATALAPYFF
                     AGWFDASNNLVSSSATYTFAMPSNNLVLSADFVAEGEEMKFGAYPQTRVTDVTLITTL
                     NNLAGGSEGLPTAENAKAWTDYLYYYNGAVTSFMWYIDLVNEGEEYRGVYYTEYRYTY
                     TDSTAGCFNQQDHGYTKRIVYWFKYEPITWKVLDIDAGKAFLLAQTVLDAQDYFYAKT
                     RRNIDGETVYANNYEHSHIRSWLNSSFLNAAFTTTEQNKIAVTTVDNSKATTINNVHN
                     FDCPNTLDKVFLLSYLDLNGHYGFVDDASRVRYPSDYALSQTVSKDYDKAYWWTRSPY
                     SSETSASRVNYNGALAAINVTDAAMGILPAIWINA"
     gene            complement(34795..35907)
                     /gene="dnaJ"
                     /locus_tag="PLU08_00820"
     CDS             complement(34795..35907)
                     /gene="dnaJ"
                     /locus_tag="PLU08_00820"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOQ70286.1"
                     /translation="MAKRDYYEVLGIKKSASKDEIKSAYRKLAKKYHPDVNKEKDAEE
                     KFKEVQEAYDVLYDDQKRATYDQFGHAAFDQNGGNPFGGGFSGSGFGFDFGDIFEGFF
                     GGGRRASRQTGPARGNDLLTRVRINFMDAINGTKIEFMHTYDKMCERCQGKGAENAND
                     IHTCSKCNGSGVETFVQESIFGRVQSQRACTSCGGSGKTISKPCKSCNGAGYTRVKEK
                     QLLNIPAGIGEGQKLRIQGKGERGLNGGPNGDLHVEIVIKPHNSFKRVGNDIYLQIPI
                     SFVDAALGVTLEVPTVYGNVEVKIPAGTQPGATLRLSGQGVKDVRGNVGNQYLEVEVK
                     TPTNLSKDQKVALEDFKKAEPKKETFFEKFKSKFKR"
     gene            complement(35975..37795)
                     /gene="dnaK"
                     /locus_tag="PLU08_00825"
     CDS             complement(35975..37795)
                     /gene="dnaK"
                     /locus_tag="PLU08_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOQ70287.1"
                     /translation="MAKKEIIIGIDLGTTNSVVSYRQADGQIKVIPTPEGKPTTPSVV
                     AFKPDGEQVNGEAAKRQAVTNPDTVMSVKRHIGTNKKFKINALKKEFTPEEISARILG
                     YMKDYAEKNLGQSIQKAVITVPAYFNDAQRQATKVAGEIAGLEVVRIINEPTAAALAY
                     GLDKEHDEKILVFDLGGGTFDVSILEIGDGTFEVIATAGDNHLGGDDWDAEIVEWIKQ
                     EIEREHGLKLNDKMAMQRFREAAEKAKIDLSQMLETTISLPFIGMGASGPISYEGKLT
                     RAKFLDLTKHLLARVEEPIRRALSDAKVKASDLNQVILIGGSTRMPAVQELVKKMTGK
                     APNLTVNPDEAVSIGAAIQGAVIAGDVKDVVLLDVTPLTLGIETMGEVMTPLITRNTT
                     IPTTKSQIFSTAADNQPSVDIVVYQGERPMARDNKLLGHFRLDGIKPARRGQPRIEVT
                     FDIDVNGIVKVTAKDLDTQKEQHITITNSSGLSKEEIDRMVKDAEEHKEADEKRKKNV
                     ELKNRAEQYINEIEHSLEEGGDKVDANQKEASEKLVTELKEAIEKEDYETLEKRLSEL
                     EQMAQMMQNMQQGQAEGETSATETPKDDDIIDADFTDKKD"
     gene            complement(37820..38410)
                     /locus_tag="PLU08_00830"
     CDS             complement(37820..38410)
                     /locus_tag="PLU08_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOQ70288.1"
                     /translation="MSDEKKHVEKDVAPKKRHLAKIQKLEAEIEKLKAESAEWHNKYM
                     RVLADMDNARKQNAKDQQYYIKYRAVPFIEKLLPALDIFNHVIREEQEDPVLNNYLTG
                     FRFVYGQLLDALTSEGLEEVVVNVGDKFDENSMHAVEVKYSEEVEPNTVLGVRNNTFK
                     FIDRLIRPAQVIVSTNIKEEPKNDDNVPEVNPEDLN"
     gene            complement(38403..39419)
                     /gene="hrcA"
                     /locus_tag="PLU08_00835"
     CDS             complement(38403..39419)
                     /gene="hrcA"
                     /locus_tag="PLU08_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOQ70289.1"
                     /translation="MRYRRRELILKYTVELFIKTAQPVGSKSLIDNYNLNYSSATVRA
                     EMNALEGMGFLEKPHASAGRVPSKKGYEFYVANLREDEVNDNFRHHIATLVEEKTKSV
                     EQVINQTCEILSQMTNLVSVALGPEKKGETLQSIQVVALDKNMGTAIFVTNRGYVEHN
                     TFIVPPSTSIQEISKVANIFNERLVGTRISEVSAKMELLQPLLTDYIVEQEVLANAFT
                     DAFIRYARDRLSLYGKESLLEQPEFINDSEKLRKLISILDDPEKIRELITGNGDLSVN
                     IGGKDEDLTFISANVHVPGDQVGQIVLVGPKRMDYSKVLWALEYVTDELEKYFEIERE
                     INDE"
     gene            complement(39544..41535)
                     /locus_tag="PLU08_00840"
     CDS             complement(39544..41535)
                     /locus_tag="PLU08_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOQ70290.1"
                     /translation="MIQDNKQSKFQKIFHEFGLYAYFIGLLLFLFALLAFIPGVSFLS
                     DKAVMILNLIALILAGYKVISEGLVETVKESIKSKRFRPNIHILMALGAIGAIIIGDY
                     GDAALLILIFAGAYFLEEYAEAKSAKEITNLLKITPTTARLLLQDGTQQEVSVESLKV
                     GDKVLVLNGDQIPTDGTIVSGSAAINEAMITGESMPKEKHEGDEVFGGSINGNSTFTM
                     VVTKNSDETVLAKILRVVSETQTNLTKTATFIKKFEPIYVTIVMLIAPLFYLFGYYVL
                     GWNEVISANGMTLALKRTIILFIGASPCALAVSDIPASLSALSLLARRGVLFKGASSL
                     ALFADVEAVAFDKTGTLTEGKPSVTDVNIPPGVSAEEAEEGKVFLVSMEKKSNHPLAT
                     AIVSHFTPIKTVDLEVEHKIGSGLRAMYDGNEYLVGKPALFNLPERCANRAKELAVQG
                     KTVVFIAKNGKPILLIALLDMIKCCAFDGLEYFRKQEINTVMITGDNELTAKAIGKEL
                     GITRIYANVLPEQKLDIVKELKKEYGGVAMVGDGINDAPALVEATIGIAMGDGTDVAI
                     DVADGVLVNNDFQKLSLMHAISRKLKRIVIQNIVLALSVVLLLIALNFVVTLPMGLTV
                     AIHEGSTILVILNGLRLLNFNEKHAIKKPKRSKFNKKIA"
     gene            complement(41540..41824)
                     /locus_tag="PLU08_00845"
     CDS             complement(41540..41824)
                     /locus_tag="PLU08_00845"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOQ70291.1"
                     /translation="MDKVVDIERLRDFFKVLGDDTRMRIILLLEESAKNVTEIANALN
                     MTQSAISHQLRTLYDHRVVTYSKAGQERIYEILDPHIYEIIKQARDHIRE"
     gene            complement(41904..42998)
                     /gene="hemW"
                     /locus_tag="PLU08_00850"
     CDS             complement(41904..42998)
                     /gene="hemW"
                     /locus_tag="PLU08_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOQ70292.1"
                     /translation="MTKRGLYVHIPFCTSLCHYCDFVKVIYQKKWVASYLEKLKHDLS
                     FFNVSRDLSTLYIGGGTPSSLSYYELRNLLELLKPYTANVKEYTVEANVESLTLPKLK
                     LMRSYGVNRLSIGVQTTDDYMLKKLNRHHTYTMIKTKIALVKKALFTNFSVDLIYGLP
                     GQTLSDLENDLMNIIKLGAPHISTYSLTIEKGTMAALKKWEVPSDDTLREMYDFILKT
                     LRNNNYIRYEVSNFALKGYESIHNKLYWENRNYYGIGLGASGYIDGKRYLISGGLTKY
                     LKGESIINYDEITSEEYVEEYLMLNLRLAQGFSLQDFKTRVGFDFLEKYGTKVKNLIE
                     RGLLNVTDDKVYATDEGIMLLDQVVLTLIM"
     gene            complement(42991..43242)
                     /locus_tag="PLU08_00855"
     CDS             complement(42991..43242)
                     /locus_tag="PLU08_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70293.1"
                     /translation="MIYLPTLAIDSPGLILLFLLLFFGLIALITFIIRRLIPGLKDKG
                     GRVNEQVAVQQELDRVLEPITDEKILAEFEKVDKETKND"
     gene            complement(43253..44734)
                     /locus_tag="PLU08_00860"
     CDS             complement(43253..44734)
                     /locus_tag="PLU08_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70294.1"
                     /translation="MSTNQGERFSNERYLTMNQVSRALGTTLIDDIWKEVLQYRNMFS
                     VTIPLRTVERNRLRVVLTPKINDRLNQLDRRISRIQARIQEVYVDANHQKALKTLLTR
                     DILKTIAASYEINFHDGIVNQLINHNISVLNAWEMFLNDYYRGLELVAKSANLDFTEA
                     LLADVGRLFNVPPLSNYIYRTTEIKTGRQVSVINHVHPHVPVNQISDAIADFYAFMAT
                     STLPTIVQSAAIIFYLDYIKPFEAHSEIIAILMAKAFLAERELALIAPYLNFELILER
                     YRDARDDVFAEVKKSADLTYFIDLLINVLNESLQTIEERIVFVVTHELEAEKHVLPPF
                     EEETQEESVSATPPTDLFEHAQAEVAEKEETFKSEPVPVYTPKKSAIPLPLPPREEGV
                     KVTPEEANLGITQLPPELSENDILRLERHIRESDPSLSRAQAYFYARHCTMGKYYSIN
                     DFKEVTGCAYETARTSMEHLANSGYYRKEKFKNKFIYTPTRKA"
     gene            complement(44813..46624)
                     /gene="lepA"
                     /locus_tag="PLU08_00865"
     CDS             complement(44813..46624)
                     /gene="lepA"
                     /locus_tag="PLU08_00865"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006506680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOQ70295.1"
                     /translation="MNYNKDLIRNFSIIAHIDHGKSTLADRLIETTGTLEKREMKAQV
                     LDSLELERERGITIKLNAVALDYLAKDGKTYRLNLIDTPGHVDFTYEVSRSLAACEGA
                     ILVVDATQGVEAQTLANMYLAIDNDLEIIPVINKVDLPSARVEEVREEIYKRIGLDPS
                     KVIGVSAKTGFNIEEVLEAIVKYVPAPSGNVKAPLKALIFDSYYDPYRGVIIMMRVKE
                     GEVRVGDEVLMMATNRKYLVTELGIRTPQEVKRDVLRAGEVGYLAAQIKDIKDISTGD
                     TVTLVNNKAKEALPGYREITPMVYCGLYPVDSKDYGDLKDALEKLSLNDAALKYEPET
                     SVALGFGFRCGFLGLLHMDVIQERLEREHDLNLILTAPSVIYHVYLTNGTMLKIDNPS
                     KLPDFTLVDRIEEPYVKAQIMTPQEYVGPIMELCQDKRGIYVDLSYLDDTRMQVTYEI
                     PLSEVIYNFFDKLKSYSKGYATLDYSLSGYKASNLAKMDILLNGQPVDALSLIVYRPT
                     AYNRGVAMCRALKNIIPRQQFEVPIQAAINNKIIARTDIKAVRKDVLAKCYGGDISRK
                     RKLLEKQKEGKKRMKLVGSVEIPQEAFMSVLSIDDDN"
BASE COUNT        13926 a         9343 c         7517 g        15956 t
ORIGIN      
        1 aagtagtgac tatcaagtta ttaaagtgtc tacatttagt tgactagtgc acttttgtga
       61 taaaacgata aattcaattg tatttataat cataatatcg aaagtgagga ttttttatgt
      121 acgaaaatga acgtttcctg cgcattagcg acaccgcaag tggttttacc gaccacagac
      181 tcaaaaagca atatttcgcg ctctttgatt ggtataaacg caatttagag tatgaaatgt
      241 ttctcgcaaa taataaactc cgctataaga ttaatcaagg ggaggtttat gagattgatt
      301 ttggccgtaa tgttggaagc gaacttaacg aaagacatta cgctgtcgta ttacatcata
      361 gtgatgttga ggcacaaaat attgtcgtcg ttcctttaac aactaagatt cattttagtt
      421 atggggaagc aattgatcta ggatatttac cagaagtaaa aacgacagaa aaaagctacg
      481 caaagattag ccaaattaga acggtcgata aagcgcgcat ttaccttcga cctattattc
      541 atactgaaga taataagccg tgcagacata cttacggccc ggtaacgaaa ttaacagcag
      601 accaatttag attagtgatt gaaggactga ataaattgct caataatcaa ttgtagtaat
      661 taattagtag ttatttagta tgtgtgaaat tgtggataac tctttgcttt gcgcaaagtt
      721 taattaaata aataatcaaa taatttttaa cttaataatc gtataatcac gctattttac
      781 atatttagtt tacataataa accttattga aagtggtata tttttcgatt ttataaccaa
      841 aaataaaaaa acaggctcat tggcctgctc ttcatcaatt taatattagt aggttatttt
      901 aaatactttt taaagcatgc attagttaac gcgttatcaa tatctttgcc cttacttatt
      961 aaataatctt tgtttgcctg taagacaatc acaaagcctt tattaatatc cttaacacat
     1021 tcagcaatat actgactact atcaaaaccg cgggaaggtt caattttatc agcagcgtaa
     1081 attacttttg ctaacttgga catcttctta tgccctgttg cgtgatactt aacagcgtcg
     1141 attaatttct tatctttctc taagtattct tctttcatta aataagcacc aacgaactga
     1201 tgatgtgacc agagtggcat attaagatac tttgggaaat gttcttccat aatttttaaa
     1261 ctttttgctg ctggtacttc tttacctata tcatggaata accctgcttt aaatgctttt
     1321 aagggatcaa catcattagc gatagcaatt aagcgactaa gactagcgac tgaaagcgaa
     1381 tgataatagc gttcatcact catccgttta gcgactaggc cactaaagta aagtttattg
     1441 ttgccaatat actctaatac aacacttggg acatcaatac tttcaaaatt acgaaccttt
     1501 gtactacttg tgctacttaa gggaccagta atttcaagca tatcaaagcg ttcaaagttt
     1561 tcatttgata ctttagaacc tttgcgttta taaacgagaa ttttagcttg ttcggcgatg
     1621 agttcaggat gtttccactg gtcaaaagcc attgcttggt cttcaccaag cagtaaatat
     1681 agttcatcgt gcggataatg ctcacgtagc cgcataaatg tgaggtaagt ataatttaat
     1741 ttaccttcat tcccttcttc aatacgcgaa agttcaaacc cttcttcatc ttttaaagcg
     1801 gcactgatca tgtttgcgcg atgattaaag ctcgtttgca cctttttcca cactgttgaa
     1861 ggtgaaagta aaaataacac aaggtcggca ccaatttcgc gttttgcgtg acgggcaatt
     1921 aagagatgac cattgtgcaa gggatcaaaa ctaccaggat atacaattat tttcataacg
     1981 ggaatttaat tttcggcgct ttaacatttt gccgatataa agtaaatgtt ttaccttttg
     2041 tttcaacaac agtagcgttc aggcgtgccg ctacttctag cgctagactt gttacttctt
     2101 tatcaacgct ttgtaagaca tggactttaa ttatttcttt agcgacaagt gctttttcta
     2161 aaagacttaa gaaactatca gtaatttcat ttttaccaag ttgatattgc gccttaagtt
     2221 tattacttag gccacgtaaa tagcgcattt ctttcttcgt taacatataa ttaaatctta
     2281 ctttcgttat aacttatggc aacaccacgc ggagcgtaaa tgcggacgac acggccacta
     2341 cctttaaaac taacaaagcc taagccgttc caactaacat catacttttt attttcatat
     2401 gttgtaaatt caaaggcgtc aagatcttgg acaccggtga caagataaga aatcggtcta
     2461 gtcttttgat gggcaatcat attattaaat gtgcgatcag cgagtttaat atgtgtacgc
     2521 gtaagtttta taccttccgc aaaatagaaa gtaaaaccag taaaaggtcc gtcaacataa
     2581 tcaaaacgcg caatcccacc gattaaaatg ctttgtttag cgagtaattg gtaagttctt
     2641 ggcttaattt ctttacgcgg cacaattacc tttacgactt ctttttcggc aaggcctaag
     2701 agactattat taatcgcaat ccctggtgta tcataaatcg ttgttgtttc actgattggg
     2761 atttgttgga ccgctagtgt ggtattagga taaatacttg tcgtaatata acgactagtt
     2821 tcatttttaa agttccgcag taatgtttta attaaactag atttcccgct gctgacagca
     2881 ccaatgaaat aaacatcacc ttttttccgg aaattagcca tttcttcgag tacttcatca
     2941 atattatagt ttcttgtact actagcgacg ataatctttt taacattaag tccttcgcgt
     3001 tcaactttag tggcgacata gtctttaagc ttagcttcat ttgtcgattt tggtaataag
     3061 tcaaatttat ttgcgactaa aattatatct agacctgaaa gagccgtatt tatttctgga
     3121 ttaaaacttg attcaaagtt aaagacatca acgacatata ccacaagggc atttttggct
     3181 ttaatatctt taattaagtt cataaaatcg agcgtgacaa ctggtgaatg gagtttatca
     3241 gcgctatagt gatgcgtttt aaaacagcgt tgacataaga aaacatcgtc gcgcttaaga
     3301 acattacttg gtacatatcc tatttcttct tcattttcac tttgaagcac gactccgcat
     3361 ccataacact ttttaatttt ctttaagttc tctgtcatct tcgttccact cctttaattt
     3421 gcccttggct cgtaatttat tgcgtagtgg gcgatcaagc aggcgattaa tacgtgtcgt
     3481 ccattgatct tcttccacta atttatctgt caacattatt ttaacaccta aggtgttccc
     3541 gcaaaagaca tccgttaata tttggtctcc aaccatcagc gtgctactta actctgcgcc
     3601 ctgctcttta aggaatttac ggagcttaaa cgcaaatggt ttacgagctc ctgaaatata
     3661 cgggacacca agtaattcac catatcgttt aacgcgttta ccactattat ttgatgctaa
     3721 aagtacttta ataccagctt tttctaagtt gcggacaaaa ttaatagttg tgtcacttgg
     3781 ttctaattca cgataactcg ctagtgtgtt atctaaatca acgagtaagg ttttaatccc
     3841 gtgcttaagg tagaagtcag cacttacttc atatatattt ttagcgtaat aattcggtgt
     3901 aaatttcttc ataatatctg tcacttctaa agtataaggt tattttatca tggacgatat
     3961 aattctcgct taacaacagt aaatcttttt ataaaagatt aaacctttaa atccttcttt
     4021 tcttataaat cgttagcgta tgtgctacaa tttagttatg aatttaagta aattaaaacc
     4081 gtatcgtaat atgctcttct tagcgcttgc cgctggtgtc ggtgctttta tgcccgtcat
     4141 tgggatcatt gttacagttg taatgtatgt aaagcgtgat gaaaactcgc ttaattttac
     4201 aaaagaagag cgttttttac taaatgccct tttaattata ttaataatat atctcacact
     4261 taatgtgctc tatactttaa aatatcccga agttaaacca gatacaagta gtgaaacttc
     4321 actttaatct tcatctaata aatcaaagat actaatttgt tcataagagt cttcaaccgg
     4381 ctctactttt acggcctctg cttttacgat tggggcataa gcttttgttt cttcgctgat
     4441 agtggggatt tcttcattaa agatactaac aatcttttct tcttcggcga gttcattaat
     4501 attgcgtttt gcatatttat caactggcgt tggcgctagg tcagttaaga gcaatggtaa
     4561 aataacattc gcatcagtta aagcatacac tcttaattct tcaatatttc gattataacg
     4621 gaaggcactg acaattgtgt gtggatcact tttaaaagtg cgcattacat gttgtaatct
     4681 tccaaggcga gcggtcgctt caaagtagct atgatcaagt aatcgtaagt gcccttcact
     4741 agtgacaata catgttctag ttctgacttc tgggtcaaca ctaatgagcg cgacgacttt
     4801 atccgctttc tttttattaa tcgatttaac accacttgct ttaacgctta caacggtaat
     4861 ttcgttttca ttatagaaac tagcagcgcc actttcagta ataactaaaa gttcactatt
     4921 tcccgttgtc agtgtgacat cagctactct gtcaccactt aatagccgca tggcttggag
     4981 cgggcgcgaa taacgaacag caacaaattc attaagactt gttcttttaa tcatgccgtt
     5041 acgggttgct attgcaaaat ataaatcttc tctaaattct ttaacattaa acgctttaat
     5101 aattttttca ttagcgttaa gttggacgat gtaattaata tgtttacctt cgtctttcca
     5161 tcttccttct ccaatttcat aaatcggtat ataaagataa ttacctaaat cagtaaacgc
     5221 tacgagaaag tcttttgtat ttacttcact tgcggcaata agtaaatcac tacttttaac
     5281 accaggtaag gcaaagtcac cactactccg atatgatttt aaactactgc gtttaatata
     5341 accgtcacgc gtaagcgcaa gcattacttg ttcgtcggcg attaagtcac gtttatcaat
     5401 ttgcacgact tcaccttttt cacgaatctt cgtacgacgg tcgtccccaa atttttgcgc
     5461 aatccgtttt aaatcacgaa taataacgtt attaagcgct ttttcattac ttaagatatt
     5521 acgcaattca tcaattgttg tttcaatggc cgctttttct tccattaata cggtaatatc
     5581 agtattactt aatttataaa gttggagcat gacaatagct tcagcttgtt cttcactaaa
     5641 gccataggcc gctttaatat tagttttcgc atttgcttta tctgtacttt tacgaataat
     5701 cgcgattatt tcattgacaa cactgattgc tttaataagt ccttcaataa tatgtaaacg
     5761 tttttcattt gtcgctagat caaattcact gcgacgcgta ataacatcaa cttggtgact
     5821 aatatatgca tcaattaaat ctaaaatatt aagcgtcttt ggcctgttat cagcgattgc
     5881 gacaatatta gcgttatagc cagcgcgtaa ccgcgtttta ctaaagatgt attctaaaat
     5941 aacatcagtg ttcgcttctt tttttagttc aataacaatc cgaattccgt ttcggtcact
     6001 ctcatcccgc acctccacaa caccgtcaac attacggttt tgttcaatcg ctccgagttc
     6061 ccgtaaaatt tgacttttgt ttgattgata tgggacttca gtgatgacaa ttctttgact
     6121 agtgcggtca ctttcaatag tggctagtgc ttccatttcc acgcggcccc gtcctgtttc
     6181 ataaatacta tctaagcctt cacctttata aatgacgcca cctgttggga agtctgggcc
     6241 taaaatatat ttacgcatat cttcaattgt tgagcgttta taagtaatgc gatgaataat
     6301 tgcgtctact acttcgcgta agttatgcgg cgggatttcg gtagccaccc caacagcaat
     6361 cccttgtgaa ccgtttacta aaagattcgg aaagcgtgat ggtaatactt ctggttcaaa
     6421 gcttgtatcg tcaaacgtta aattcatatt aacagtcttt ttattaatat ctaacactag
     6481 ttcattagaa agttcgttta agcgtgattc agtgtaacga taggctgctg gttcatcacc
     6541 atcaatacta ccgttattac cttggaaatc aataagcgga tagcgcatgc tccattcttg
     6601 cgacatacgc gcgagcgcat catatatact agtgtcacca tgtgggtgat atttccccat
     6661 caccgccccc acggtatgcg cacacttttt catcggttta ttaaaagtat tgccttcgtc
     6721 atgcattgca taaattatcc gccgttggac gggctttaag ccgtcacgtg catcaggaat
     6781 cgcccgattt tgaatgacgt attttgaata attgccaaaa cggtcaccca taatttcatc
     6841 cattggtaat tcaataattg tttcggtgat tacttgttct tcagtttgtt tttttggttt
     6901 tcgttttgac atcttatttc acctcatcga taaatgtatc atatgcgtta aagtcaacat
     6961 tttcttcaat ccatttgcgt ctaacattcg catcattccc catcaaggta ctaacacgca
     7021 tctctacgag taaaggatcg ttaatattaa cgcgaattaa tgttcttgtt tcgggattaa
     7081 gtgttgtttc ccataattga tcagcgttca tttcccctaa acctttataa cgcgtaattt
     7141 tataaccggg accaattttt tctttcgctg cttctaaact ttggtcatcc cacgcgtatt
     7201 caaagataag ttttttattc acttgtttac tcacacgata taaaggcggc acggcaatat
     7261 aaatcatccc cgcttttaaa agtggtttca tatagtgata gaaaaaggtt aataataatg
     7321 tttgaatatg tgaaccgtca taatcagcgt ccgtcataat aatgattttg ccgtaatggg
     7381 catcttcaat atcaaaattt tggccaaagc cagcaccaat agtgctaatt agtgtcgcaa
     7441 actcttcgtt ttttagcatt ttatcaacag tacttgtatc agtgttaagt ggtttaccac
     7501 gaagtggtaa cactgcttga tacatccggt cacgtccgct tttagcagta ccaccagcac
     7561 tattaccttc aacgataaag agttcatttt tcttataatc tttactttgc gctggtgtaa
     7621 gtttaccgct taaaatcatg tcattattcg taactttttt cgttttgcgc aagttttctt
     7681 ttgctttacg cgcggcgatt ctagcgtttt gcgcatcaac acatttttta attaaactaa
     7741 cggcgaattc atgattttct tgtaagtagt aagtaagctt agtgtaaata aaactattta
     7801 gtgcccagtt tgcttcattt gtgcctaact ttccttttgt ttggctttca aattgtaaaa
     7861 tgctttctgg aatacgtaca ctgataattg ccgtaagtcc ttcacggatg tcgttaccat
     7921 caagtttcac gcctttacgt agtaaattat gttcagttgc aaagtcatta acagcgcgtg
     7981 ttaatcctgt tctaaaccct gtttcatgtg taccgccatc atgcgtgcgt aaattattaa
     8041 cataggagag gacagtttca ccataatcat cgctacaata ttggagcgct acttcaattt
     8101 ccattggttg acttaattta tcattattcg tataaatatc ttcaaagtaa attggtttac
     8161 taagcgcggt tttattacga ttaatgatat caacatactc aattaagcct ttttcataat
     8221 aaaactcttc acttaaacca cttctttcat ctttaacaat aaaagcgact ttattcagta
     8281 agaaggcact ttcttgtaag tgacttgtaa ttgtatccca tttaaattct aaattaccaa
     8341 agattttagg gtcaggttta aagttcacaa ttgtcccggt gcgattagtg ttccccacta
     8401 cttctaaggg agtgactagt ttaccgccat tttcataacg gagatgatgc acttttcctt
     8461 cacgatatac agtcacatca agccattcgc ttaacgcatt tgttacacta gcgccgacac
     8521 cgtggagacc cgcacttgtt ttataagttg attcactaaa tttaccacca gcgtgtaatg
     8581 tggaaaatac gaggtgtacc gctggttgtc cggtttgttt attaatgcct gtggggatcc
     8641 cgcgtccttc gtcagcaatc gtaattgagc cgtttttatg aattgtaaca gtgatcttat
     8701 caccataacc actcattgct tcgtctgtac tattattgac gacttcccag acgagttggt
     8761 gaaaaccaac aatgtttgtt gacccgatat acatcccagg tcttttgcgt accgcatcga
     8821 gtccttctaa aacttgaatc gaatcagcgg tatatgaatt tgtaatattc ttatctgcca
     8881 tttgattttt ccttatcatt tcacaatact ttgttattat aacaaaaaat tactttattt
     8941 tataattgga ctattttaat ttatactatt taagggaggt tacttatgca tccatttata
     9001 aagttcttaa tattcttagg attcggactc tttggctact taattggctc gattaataac
     9061 gctgtcatta ttagtaatct cttttttaaa cgtgatgtca gaaattatgg cagtaagaat
     9121 gcgggcggga caaatactgg tcgtgtcttc ggccgtaaag caggaatcac tgtaatggtg
     9181 cttgatattt taaaaagtgt ttttgtatat tgggcgataa ccctagtctt ccacttcact
     9241 aatgtgggac tttatgttga tcacgcagca acactttact ttgcaatgct tttagtcgcg
     9301 cttggccact gttatccagt gtattataaa tttaaaggcg gtaaagctgt gagtgtcgtc
     9361 gctggtttta ttcttgccac aaactgggta tttacgattt taggggcgct tattttcttt
     9421 ttaattctta aattacgcaa gatggtcagc tatgcatcga tcttaatggc cgctattatc
     9481 gttgcactaa caccaatttt atttatccca gtacttggtc gtattagttt ttatccgctc
     9541 gtcgtcgata atctttatta ttacatcccg acactagtcg gtttatgtat cttattaatt
     9601 ttaaaacacc gcacaaatat tgaacggctc caaaaaggca ctgaatcaaa aattacgtgg
     9661 ataaagtaat tcttgccact aataaataaa agctaggacg tgtgtcctag cgtttttatt
     9721 tacatataat cttaataatt aagattgttg cattccgcgc ataatttcgc ggacttgttt
     9781 ttcacttggt ttacggccca tcatcgttaa cattgtccga atttgttttt ccgtaatcgg
     9841 tggatttttc tttaattgtt tttcaaatac tttacgacta atgagataac ctaataccgc
     9901 acctagaatt agcgcaaaga ctgctaaacc tatcgctgct ccaattggca ttatgctctc
     9961 ccatcgtaac taccgtcagt tgttttgacc ataattactt ctccttcttc aataaataat
    10021 gggacactga ctcttagtcc tgtttccaca atcgcgtcct ttgtcgcgtt ttgtacggtg
    10081 ttacctttaa cacctggcgc acattcggta attttaaggg cgactttagg cgctaaagtt
    10141 atgccaaaaa tttccccttc ataaccggcc atttctactt cactattggg gacaataaag
    10201 ttaagttccc aagttaatga ttctttggat aattcaactt gttcgaaagt ttcagtattc
    10261 ataaacacta agaaatctcc actatcataa agataagtca ttttgtgttt gctaatacta
    10321 atcctgtcaa tttgataacc gctgttgtat gtttgttcgg taatcgcacc agtgcggaca
    10381 tttttaactt taagtttcgc aatcatttta cgcattgctg tcttattaag taagatgtca
    10441 agcacgttat aaatttcacc agcttcggta aaataattac cagggcgtaa ttctgtaaca
    10501 ttaatcataa tttaaccctc ctctaattaa ttttaaatag tctagcgttt ttcgcggtgt
    10561 agcgtttgtt tattgcaacg tgggcaaaat ttcatttttt caatccgttc gggattgttt
    10621 ttcctgtttt tactggaaag ataattttct tcattacatt ccgtacatct taattgaata
    10681 tttacacgca taattcttca tctcctctat ttgcttgtat atcatatcat aaatttagaa
    10741 agtattacta aaagtaatat aattcgtctt atttttcccc taattcatct ttaagcgtag
    10801 tgttccatac tttattttta atcattgtaa tctcatgacc ataaggcgcg accccattaa
    10861 tataaggtgt ttctaagatt ttcgggaggt gtgctaagcg gggactataa atatatttca
    10921 ttaaagtatc atagccaatc gtcccaaagc cgatgttttc atggcggtct ttagcggcgc
    10981 cacgctcatt tttggaatca ttaacatgca cgacttttaa gtattcaaga ccaatcacac
    11041 ggtcaaattc atctaagacg ccatcaatat catggacatc atatcctgca tcattaatat
    11101 gacatgtatc aagacagacg cctagttggc tctttttatg aacgccatca ataattgtgc
    11161 gaatttcttc gaaggtacgg ccaatttccg tgcctttgcc tgccattgtt tctaacgcta
    11221 tgatcgtccc acttgtgtca ttatcaagca cagtattaag gcctttaata atttgttcag
    11281 taccggcttc cacacctgca cccacgtgtg aaccaggatg aagcacaacc attgtaatcc
    11341 caaaatcagc aacgcgttgt aattcatctt gtaaaaaaga taccgctagt tcataagttt
    11401 caggttttac actgttgcct aaattaataa tataaggggc atgagccacg atatgcttaa
    11461 tatccatccc tttttctttt aaatatttaa taccttcaag tgcatgcatt tgctcagtcg
    11521 ctaagcgtct tgtattttgc ggtgcaccag tataaatcat aaatgtattt gcgccatagc
    11581 ttaaagcttc attaattgcg ccaatataat aatcgggagc actcatccca acatgactac
    11641 ctaaaattaa tttatcactc atgaatccct ccttttttcc gctttataac gttcgacacg
    11701 ttgttcacgg attgactttt taatcatctt acggcgatgt tttcttttta cattttccac
    11761 cgctgttcta actttctttt tatagccggg tcgcactttc tttgtttttg tctcggcgac
    11821 cgctgtttta attttaacat taagttcttt ttcttccgga gttagtgctt tcttgcgact
    11881 aatgcgcttg gttacagcac taaaagcgcc acctttatac acaagacgcg taaatttaac
    11941 acctaacttc tctacttttt cgactgtttc taagtcatca tgatcgtaaa gcgtataagc
    12001 attaccttta ttatctaaac gccctgttct acccgcacgg tggaagtaaa attctaaatc
    12061 aaaaggaaaa ctaacgctaa gaacatcact aacatgttca atatcaatcc cgcgcgctgc
    12121 aatatcactc gcaacaacaa cttggaattc attattacgc acccgtctaa acattgcttt
    12181 acgtttacgg ctttcaagct caccagttaa aagtccgatt ttatgacctt ttgcacttaa
    12241 aaactcataa agttcaagca cttctttatt cgtggagcaa aacactagta ataaataagg
    12301 attaaagtgt ttaacaaaac tttcgacaac ttcaaagcga ttcatatggt gtacatcaat
    12361 taagtgatgg gtgacgttat cacttgtttt atcatcgcgg tcaatatcaa tcacggtctt
    12421 tacacctgtc tttaaagtaa caaggttcgc tagcttttgc ggaatagtcg ccgagaaaag
    12481 ctggacagta ggattatcaa gcgctgctaa agtttcggaa ataacatcgt aaaaacctga
    12541 atctaaaagc atatcacctt catcgagcac gacagtatga attgatttaa aacttgtgat
    12601 atcttcgtta attaataaag cttttaatcg gccaggcgtc gcaataatta gcgctggttt
    12661 actcgctaaa cgttctttat gacgactagt ttggagtccg ccggcaagaa acaatatatc
    12721 tactccttta tagtcatcat ttaaaacacg agcaaaatca tagatttgcc gcgctaattc
    12781 gcgggttggt gctaagatca gtgcttgaag ttcttcggtc ttctctaatt tatttaaaat
    12841 tggtattaaa taactatgag ttttcccgct acccgtcttc gctttaacga tgagtgattc
    12901 accttttaaa gcgcgtggca atatttcgct ttgcacaccg gtcattgtta cataaccgtg
    12961 catttttaaa gtgtccacta atcttttatc aatattgtat ttaccaaact ccataatatc
    13021 aattccttat tatctactac ataactacta atttattact tataaaatac ttttaccgtt
    13081 attttataac aactggcggt ttttcgtgct taattgcaat tacttcaaaa cttggatcaa
    13141 ttgttagtaa gacttctttc attttccata agaatgcatt ctctatttca tggggtacat
    13201 ctaaataatt gtagccagca ttcattacat cacggcgcac ataatgcggt gcgtcaccac
    13261 ttatatacat gtcgtagcct tcagccattg ctgctggcca atcgcgactt ccgccaccac
    13321 caatgatagc gactcgctta atttcgcgct ccttatcacc agcgactagt aagccgtaag
    13381 ttgcttctag tttatcaaca atatagttag cagcttcttc aactgtcatt gggtacggta
    13441 aataaccgcc gcgcgccatt ggttcgctgg ctaatggttt aacggcgctt aattctaatt
    13501 tttccgcgag ggcatcattc atcccgtttg gtgcttcatc aaagttcgta tgcattgaat
    13561 aaacagtcac gggtaaagta tcaagccata gcgcaaatgc ttttttcttc tcatcgcgcg
    13621 caaacacttt agcgcgtgta cccctaatga acggatgatg ggttaaaatt aagtcaggtt
    13681 tatgacttaa tgcttcttca aggacaattt catctaaatc aagtagtaac acaatgcgtt
    13741 taacttcttc gactttttgc cctgccatat agccaacata atcattatgt ttaagggcga
    13801 tacgctttgg ataaagacga ccaagtttag ttagtaatgt gcgtgtcttc atactttttc
    13861 tccttcacta ataacttttt caataagttc ttgttctttt agcaactctt ctttacgact
    13921 tagcggtaac tttttattac ttaagatgcg tttattttgg ttataaacac tagtccacat
    13981 cttaataaag ttttcacttc tcgcttgtaa gtgataagtg ccaaaatacg gattaacttc
    14041 ttttgtcgct atcgcttttt gctcggcaac aattatttcg taaaacactt ctttctcgac
    14101 aacgatttct tcgtagctaa ttgaatagtt taacgcattt aatacttcgc gcactgcttt
    14161 agcgttacag tgcggcgcta aaatgagttt ttggacatta actaacttag cgggatgcgc
    14221 ctgtaagata cttacaatta attcgccacc cataccagcg ataacgacag tgttaatgcg
    14281 tgcaggtaag ttttcaaggc cgtcggctaa atcgatttca ataatgtcac tcttataagc
    14341 tttaagttcg ctttttaacc ggttgtatgg tcctttttta ttttcgctcg catataaagc
    14401 acgatgtcct ttaatcgcta aataattaac gagtaaaccg tggtcactcc caatatcagc
    14461 gacaacatca tcttttttaa ttaaattggc aattgcttgt aaacgcagcg ataacattat
    14521 tggatatgtc cgcgtaaccc tttcgtacgc gaaggatgac gtaatttttt aatcgcttta
    14581 gcttcaattt gacgaatccg ttctcgtgta acaccaaaaa tcttcccaac ttcttcaagt
    14641 gtccgcggtt tatcatcatc aaagccgtag cgtaagtgta acacacgttc ttcgcgtggt
    14701 gtgagaaatt cttgcatgtt atcacgtaat tgatcgtgta aaatgctctt tctaacaaag
    14761 tcggttggtg tttcattatc tttatcttcg acaaaatcac ctaaatgact atcatcttct
    14821 tcaccgattg gtgattctaa acttactggt tccatcgcaa ttgcttgaat ttcacgaatt
    14881 ctcgcaggtg taaattcacg gaatgattca cttacttctt ctggtgttgc tggccgttta
    14941 agttcttcag ttaaagtttc aactttcgcc ataatagtac gcactttatt ggcgacttct
    15001 tccggaatcg gattttcttc ggttgcgtta gcgcttaatt ccattgcttc ttcaacatcc
    15061 ttaacgctat atttaattaa agcatcacta atttcttcag gtgtagcgtc gcgcccaaat
    15121 tcttgggtaa ggcggcgctg tgtccggcta attttattaa ttgtttcaac catatgaaca
    15181 ggaatacgaa tagtgcgggc ttggtccgcg attgaacgac taatcgactg cttaatccac
    15241 caggttgcat aagttgagaa cttaaaaccc tttgtgtaat caaacttttc gacagctttc
    15301 attagcccaa tattaccttc ttgaattaaa tcaagaatat gcatgttgcc gctgctgtat
    15361 tttttggctt cattaaaaac aagtcgtaag ttgcgagtaa ttaattcatc acgcgcatcg
    15421 atatcgcctt cttctttata acgacgggca agtgttactt cttcgtcagc tttaagtaaa
    15481 ctatagcgcc ccatgtcttt catatagatt ttaacggagt ctgttgtttt aacttcaccg
    15541 agtgaaattt tacgtaaccg ttgattttct tcaaagtttt cttctttaac atctttaaag
    15601 gcatcaccaa atgataattc ttcttcatca aaatcatcat caagatcaag atcatcatct
    15661 aaatcatcgt caccatcaac aaaattagcg aaatctggat catattcatc gctttcaata
    15721 tcactatgaa tagtcacttt gttattttta aagtattgaa taagtttatc aaactcattt
    15781 tcgcttaagt ctaggtgtga agtcgcatcc ataacatcgt cttggtcgag ctcattatta
    15841 ttacggcgcg cttcttcaag tagaaacttc cgaatctgcg gtaatgttaa ctcaatttcc
    15901 acttcatcat cttcttcaat cgctacttcc tctaattcag cgaggtcttt taatttcttg
    15961 tcatccataa actatcgtct cctttactat ttcttctttc catatttttg cattaattca
    16021 gttttagctt gaatgtgctt aactttaagg gcggcataac tcgcttcatc aggcgcacgt
    16081 aagagttcgt cctgatgttt gcgttctaag atttgtaatt cgcgttcata attaagcgtc
    16141 ttagcggcgt catttaatat attttcatcg tatttcacat cttgcaggcc actttcaatc
    16201 gtgagtagct cttgcgtata ttcattaata gtagcttcat cagtgaaacg tgactgaata
    16261 tcatttaata aaagcgcata attaacttgc tcttttggtt taaattcttt taagtaatta
    16321 acgatatagc ggtgtacttc atgcgtaaac acgcgtacat ggttatcaaa gaaagtgcgt
    16381 gcttcgggat acacaagcat ttgataaaca ataaagcgtt ctgcctgcgc gactttattt
    16441 aatgtttgcc gcaccggtaa cttttgttta acagtattta tcgcttcgtt tcttgtttgt
    16501 tttttacgcc gtaagctttc atacatatca gtgagcacta aactattaaa gccgcttgct
    16561 tcggcaagtc tttcaatcat cgcacttact tcaagacggc tatttagttt taagatgatt
    16621 tcattcataa catcattaac aaacttcttt gtactagcca tatcatcaag ctttgtaact
    16681 gataagtaat aattaaaaat aaagtcatca cgcggaataa aggttgttaa ggctgttaca
    16741 agcgcttctt tcccttcttt atttaatatg tcatcaggat catagccgtc tttatgtggt
    16801 tttacgattt gcagcggtat attcgctgcg attaaatctt taatcacact tagcgtcgcc
    16861 atttgaccag cagtgtcact atctaaataa attcttactt ccttattaag taagcgtaaa
    16921 agcgctaatt gttctttcgt aaatgctgtc cccattaagg ctacaacact gcgttcacca
    16981 acgcggtcaa gagcaatcac atcaagaaac ccttcgacga gatacacata ttgcgctgcc
    17041 atcagtgctt ttgcgtgatg gtagttataa agtgtatccg acttaataaa taattgtgtc
    17101 tcacgcgtat taacatattt tggtgctttc ttttcgctag gcaacgctct tgcgctataa
    17161 gcgacaactt gtccgtcttt attattaagc gggaaaataa ttcgcccgtg attaatatca
    17221 taaggtcgct caagactacc acctaaaata ccaatatctt cgatcgtttt aagggcgtga
    17281 cctttacttt gtaaaaactt aatagtcgct tcaccgtcgc gcggggcaaa cccaatctta
    17341 tattttttaa gtaaggcatc atcaagcccg cgattaacga gatacttttt agcttcacta
    17401 cctgcttccg taattaaggt atactcgtaa taagcagtta aatcattaat cgcactgata
    17461 agcgggtcaa gctttgtatc gcgcacaaca aacttttgtt tttttgtaaa aaagtcatgc
    17521 ttaaaactaa ccatatccgc gagccgttga gcagcttcat agtatgtctt tttttcgtaa
    17581 agcgctacaa agcgaatcgc attaccgccg gtattacata cgaagcaatt aaagatttgc
    17641 tttgatggcg aaatcattag actaggatta cggtcatcgt gaaatggaca tagtgcacta
    17701 tagttttggc cttgtttatg gagcggaata taacggccaa tgacatcaac tatgtcaaca
    17761 ttttcgatta atagatcata agggctgcga gtactaataa gctaaacctc ctaaattact
    17821 actaattaat tactgaaatt ttacttttga gtgtaaataa gcttttaact cacttaatgg
    17881 taagcgaatc tgtgccattg agtcgcgttc acgaattgtg acgctttcat cttctaaagt
    17941 gtcaaagtca atcgtgacgc agtagggtgt accgattgca tcttgacggc gatagcgttt
    18001 accaattgag gcggtttcgt catagacagt actaaagtct tttactaagt cactgaagac
    18061 tgcaagtgct ttttcactta acttccgttg taagggtaat accgctactt gataaggggc
    18121 taagtgcggg gcaaggcgca taacaatccg ggtatctttt tctagttctt cttcgtcata
    18181 actatcaacg agagtcatga gtaataagcg atcaagccca acactaggtt cgacacaata
    18241 tgggatatag cgctcattcg tatctgggtc taaatattca aagttttcac cagaatgttc
    18301 catatgttgc tttaagtcgt aatctgtgcg gtcagcaatc ccccagattt caccccagcc
    18361 aaatgggaat aagtattcaa tatcggttgt tgctaaacta taaaagctta gttcatcttt
    18421 cgtgtggtca cggaacctta agtattcagg acgaatccct aagtcttgca caaatttaat
    18481 acaataatct ttccaatatt taaaccattc taagtcagta cctggtttac agaagaattc
    18541 taattccatc tgcgtaaatt cacgggttct aaaagtaaaa gtacctggtg taatttcatt
    18601 acggaacgct ttaccaatac ccgcgatacc aaacgggagt tttttgcgca tcgtacgttg
    18661 cacattttta aagttaacaa acatcccttg cgctgtttca ggccgaagat aaacttcact
    18721 ttgactatct tcagtaaccc cgatatgtgt tttaaacatc atattaaatt gacgaatatc
    18781 agtaaaatct tgtttaccac atactgtaca cttaacacca tactctttaa tgattttcat
    18841 catttcttcg tttggtaagc ccgtaacatt aagtccttta acttgatctt caattaagtt
    18901 atcagcgcga tgacgagctt tacagtttcg acaatcaatc agcgggtcgt taaaggttgt
    18961 gacgtgacct gtcgcgtgcc atactttcgg attcattaat attgctgcgt caatcccgac
    19021 gttatacggt gattcagtaa caaacttctt ccaccataaa ttgctaatgt tatttttaac
    19081 cgatgttcca agtgggccgt agtcccatgt attacttagt ccaccataga tttcactacc
    19141 ttggaacaca aagccacttg ttaccaagtg atttactatt ttttcaaatg aatgtttatg
    19201 cataattcct ccacaattaa agaaaagtat attattaaaa tacatttttg tcaacccact
    19261 atgagtgtag ttagattatt ttattaaatc tttactcata attttataat tataagtaaa
    19321 ttctaagtat gtaagcatca aggttaagag tgaaagtgcg gcttcatcgc taaattcgtg
    19381 acgcaagagt tcttcttcat taatcataaa tccgtagcgt aacatttgta tttcttcagc
    19441 gtttaaggtt attgtcttct catcgtagca ctttttacaa atgagtccgc ctttattaaa
    19501 agacacaccg atgatacttt tagtgtcccc acaatttaca cagcggtcaa ttactaaagc
    19561 actccctaat atgcgtaata aattcgtagt aaaaagatac actaacgaaa ttagttttgt
    19621 tgtttcactt agcgacatta acactttttt tataagcgga aacgcctcac taatcgaatc
    19681 attgtctttt agtgttttac taatgacttc accgatgata tttaaagcga taatacgctt
    19741 ttcgtcagta aaaactttag cgatattcgt taaactttcg acatttgtta aagtatgtcc
    19801 tgctttactt tcgcgtaact ccacatcaac taaagcatat aaaaagagct tacccgcaaa
    19861 ctttgattta acattaagtg ctccagtagc gcgaaaagtg ataatgcctt cttccttact
    19921 taaagcatta acaatataat ctttttcccg gtacggtgtt aaacgaataa tgactacttc
    19981 catctttagc ctaataattt ctttaatatc tttgggtcac ttcgccataa cggttcaact
    20041 ttaaccatta attctagtaa cacatcttta cctaaaaact ttgataattc atattgcgtt
    20101 tgtaaacgga tgcgtttaat cattttcgct cctttaccaa taagaatgcc cttttgacta
    20161 tctttttcgc aataaattgt taaataagct tcaactttag tgtcactctc tgttaaatga
    20221 tcgacaacaa ctgcgacact ataaggaatt tcttcgcgtg ttaacataat aatcttttca
    20281 cgaacgattt cattaatata attttctaaa cgaatcgcgt aatcttcggg caacggaaaa
    20341 taaagtggcc ctacttctaa atgttcaaag attaatgtta taagtgcatc aatattaaaa
    20401 ttacgcacac tgcttacttc aataagtggt gtatccggat aattttgtgt ccattgctct
    20461 ttaacttttt gcacgtgaat gggagaagct aaatcaatct ttgttaagac gataattaaa
    20521 ggatgatcaa ctttaagccg actaaataaa tattcatcac ccgcaccata ctccacacta
    20581 gcgtctacga ctaaaagtgt tacttcactc ccacgaatac tctgataagc ttgtttattt
    20641 aaatattcac cgtatggtgt ctttggttta tgaatacccg gtgtatcaat aaaaacaatt
    20701 tgaccacgta agtcgttata cacaccttga atatttttat tagttgtttg cggttttggg
    20761 gtaacaatcg ctcttttttc acctaaaatc gtgtttaaaa gtgtcgattt accggcatta
    20821 ggtcgtccag caatggaaac aaagccactt ttaatcataa gtcactatca tcaaaggcga
    20881 atggtaaaag ttccgcaatt gttgtttcta aaaatgcatc ttctaaattt gttaagtaaa
    20941 tcggagcgtc ttttggtaaa agttcactca tcacttgccg gcacgctcca cacgggctaa
    21001 ttggtcccgc tgttttacca gtgaccgcta gtgcgacaaa gtcttctttc ttttcgccat
    21061 tcatcattgc gtgaaataaa gcgtttcgct ccgcacaatt acataatccg taactagcat
    21121 tttcaacatt cgcgccgaca tatactttcc cgctttttgt taatagcgct gcgcctactt
    21181 taaatttaga ataaggaaca taggctaagg cacgtgcttc tcgtgctttt ttaattaact
    21241 cttcttttgt cactttttcc ctcctctttt accaataatt tggtcttgca aagtgatcat
    21301 cacctgctct tctttttcat ttgtgtgatc ataccctaat aaatgtaaga gtccgtgcgt
    21361 aaataagaat ttaaattcac gccgggcatc gtgtcctaat tcaagcgctt gacgttgcgc
    21421 tactgggaca caaataataa tggaacctaa atgtacaaag ggttctttaa acttatgacc
    21481 aggtttaata tcattaaagg caaatgataa tacatcagtt gctgcatctt tagcgcgata
    21541 ttctttattt aatgccctaa tcctttcttc atcagtaatt gtaacttcaa caaggaattc
    21601 acctgtcata taatcaagta atttcattgt ttttgcataa attcgtttaa aaatagcccg
    21661 atagttttta taagtcggtg cataatcatc ataaaattca acagtaagtt taagcatcat
    21721 aataatttta ccatagattg attaaattaa cgcagtgctt taatcgagta ataaagtaaa
    21781 ccactaaagt ggtatttgaa atatacatag tacaatgtta agataatgta tataaaattc
    21841 ttatttgtta cattcttaaa aacaaagcat taaaagaaag gaaatagcaa tgaaatctga
    21901 ctttaccttt atatattccg caccaaaagt tacagatgaa aaattaccag cggttttttt
    21961 atttcacggc tatcgaagcg atgaaaaaga tttactttca cttgttgcaa gtttagaaag
    22021 cacccatcat atttttagtg tgcgcggacc gctcagtttc atgtttggtg gatacgcatt
    22081 ttgggacatg caagacttag agaaccctaa tcttgaacaa tatcaaaacg cagtggaaag
    22141 tgtacaaaat tttatagtta atgctatcac tcaatataat ttagaaaaga aagacatcac
    22201 gcttttaggt tttagtcaag gcgcagtact tgtgcaatca attgcgctca gggcacaaaa
    22261 actagtgaat aaagtcgtag cactaagtgg tcatcttcct tattttgcta aggatgaaga
    22321 atatgcgaca ctaaatctta acaaactaca tatttttatc tctaacggca ttaatgataa
    22381 tgttgtgcct tacgcttggg gtaagggaag cgcggactat tttaagaagc gcggcgctga
    22441 tgtgactttt aagtcttatt ttagtggcca ctatgtttcg cgtgacaacg cccgcgattt
    22501 aatcgatttt atagtaagta attaatagtt aatcatacat cttttaatta accggaatat
    22561 cataaatagc gttgattgca ctataataat gatgagtatt cagcgctaaa ctaaagagct
    22621 ttttaagttc atataagtaa taactgaagc caaagaaata aaatattaag aaattaagtg
    22681 aataaacagc ggtaaaatac attaagacaa agattaagac cccaagacta aaatgaataa
    22741 taatcgaacg atatacccgc cacgcggcaa tatgcgttga atcattaatg atttccttat
    22801 ataactgcat tcttttttca ttattttctt ttgctaaact acttaattta acttctaatt
    22861 caaatgtttt agcgctacct ttaatccccc ttttaacttc aattaatgct acaatcatca
    22921 aaagcacact gatgattaca aagtagtaat tattaagcac taaaataatc gttataagta
    22981 atgtgctaga gatagcacta aaaagttgta aaggtctact aaaaacaaca gttttagcct
    23041 cttgcaatac tttaaactta tacttaaagt tatctttaat atttacgagg cgtggcgcta
    23101 tatataagtc atcgaacttt tgtaagttct tcattattac ttgttgttca ataatcttta
    23161 atatcgctgc taaagtaaat gacacataac ttattaaatc taaacttgca tgacctagga
    23221 aagcaaaacc aattaataaa aagattaaaa acaaagagtg aaagattaat gcaccatatc
    23281 gttgtttgaa tttgactaaa ggtggatttt caaccttctc tacacaatta actactaata
    23341 tattactaat aaaatacttt ttaatgtttt ttagacttaa aacataaaat tgaccacttg
    23401 gatcagcaat gctgagtttg ccctttttaa actctgctag gacgaaatga ttcgtatcat
    23461 ctacttaaat gcatcacaaa aggtcccttt actttcttta cttcttcaat cgcgtcaagc
    23521 ttataaccaa gtaactctac accatactct tttgcctttt tactcaaagc tagaaagtta
    23581 tcaaagtccc cacttaaagg taattttaaa tagcgcttat cacgatgagt aatcgcaagc
    23641 agcatacgga gcactgcttt accgcattca ttagtgttat tttgggttaa tcttacatca
    23701 attaggttca cacttataat tacaacctaa tcgtttattt tggtacaaaa aaagagaaga
    23761 atgtgacttc ctctcttttt gcgctaaatt aattagcctc tgcgggcttt acgacgagct
    23821 tgttccgctt taagtttgcg gcgaagccct ggtttaacat agtattgacg ttttctagtt
    23881 tcagaaagga cacccgcttt atttacttgt cgtttaaagc gtcgtaatgc ttcgtctaat
    23941 gtttcgtttt cacgaacgac ggtttttggc atgctcttca catcctttct tggctagttg
    24001 ccattaatta ttatagttaa ttattaattt aatgcaagaa aaagcataat aagaaaaact
    24061 tagtatgcta aacgaattta atgtgttatt aacctgttat ttaaagttct tattatcaag
    24121 ttcaatatta ttctttttca atatgtaatt gtttaaatat atcctgctga tattgtacca
    24181 aatacttcta aatattatct aatagtaatt tttcttattt ttgcaatgtt ctaatatttt
    24241 attaatttgt ttacgtgata aatcgttaca atggattcgt tttactgaac cgtctaaata
    24301 attaaattca ataaatttat gacctgttct ataagtttcg atacctctac ctcttttttc
    24361 tcggctgcgc gaagaaaatt tgtaatctcg ttgttgtact tcaacaaaag aaacatcttt
    24421 aagatcaatg ttatacttat actccacctt aagaaggccg acaagtaaat cgccactgac
    24481 aaaaatagaa gactctttta aaataattat tttgattgca aataatatta aaaatattga
    24541 tatcggaatt agaattataa aaaagaattt tgtccgccac aaaaaaatat ctaagagcgc
    24601 aaaaagcaaa aaccaattaa cgaaataatc attttaatta gcgaattaaa aggaaatata
    24661 taatctttca tttatcacac ttcaatacta catttaatta gcaattaaag atatcaatga
    24721 aactattatc gaaattacca aagaacttac taaaaaaaat aaaattcggc gcgaattaaa
    24781 atacgttatt tttgcattta aacatttttt cttaatattg ataaccatca atagcggtgg
    24841 gttttctatc gaaattaagt acttgtgttc tttatcaaaa agcactaact ctgtcgggct
    24901 tggttgacca tctatcgcac cgaaattata tgtcagataa tatacatctt taagatatat
    24961 tgtgctgtgc acctcattat ttatttgggt aattaaagaa tcgctagtaa ttatataagc
    25021 tgtttttata aatatttttg caattgccgc aataatgtag aaaatgatat tgactataac
    25081 aatcactaat atcgttaaac cagtgaactt aataaaatct ttaaaaataa gcggggggaa
    25141 taataataat gaagaaatta aataaattgg gataaaagta attattagtt cactaattac
    25201 acctgcaacc ttaatcatta tattccgctt atattttttc atgaaaccta ttcctttaat
    25261 aatatcttca taatcttttt tatttgacac tgagcatagt taccaacata tacatattta
    25321 gtgccaccat ttttgattat aatttctaaa tatttattgc gtatgtgctt ataaaatacc
    25381 ttgtgctttt tttcttcttt cgtcaattta acaatgttta ctgcgagtac ttctgatata
    25441 aaaagttctt ggttccacct aatatcaata ttatttatca ttttttttcc acgtttttgc
    25501 atgcggaaaa taatagaaaa acacataatg attattcatt attaatgtat agccagtaat
    25561 ccaaaaaata ccaaccacag gaagtgaaac ccccaaggac gcaaagatta ttaaatacca
    25621 gttcttgcat aaccactcaa gattgcttgt atcagcaaaa acaaaaaaga atattgaaca
    25681 tatcgtaaag ggtgtgctaa tgatagcaac aatcagcaca tcataaaatg caattattcg
    25741 ttttttcata aatctcctat gtggcaattt tatcaattaa ttgaccagtt attaaatatt
    25801 tggcattatt tcttactccg tgattgagcg aacttgtttg aaatactctt ccaccttcat
    25861 tagaagcgta tgctcctcca cttgtacctt gtccgtagcg ctttactaag ttgccataat
    25921 catcatattc attatttgct gaaataatac catgcgtttt tttaattata ttatcgtttt
    25981 catcatattc ataacttgtt acaattggat aactttttac gataatgtcc gctaggtaga
    26041 tggtcgtgtt tagattgctt ccaccattaa gtactattcg tacttcatca aatatatgtc
    26101 tggccgtaat ttcagtagtt aataagttat aataacttcg tggtcatcac taaggcatca
    26161 acacttttaa ttaatgtctt tatttcttta tttgtttttt ccttaattag tgactgatat
    26221 aaaaaaagag tggaatcccc tatttttgtg gtttccacat aatcatcaat aaagtttatt
    26281 gttctgacaa taactttttc ttcactattt tttggttcta cttaatttct aattaacgag
    26341 taatatcgat taatatcata atcaccacta ttaacggact ttaagagatt taagagcagt
    26401 ttataatgtc tttttttgat gttcttttca ctatatttat atttaagttt taagtcatta
    26461 attgtatata ttttcatctc tttctcctaa cttaaatata gcataagaac taaatttttc
    26521 aaagataatt taggttaata taaaccagta aaattattga taccacaatt agataagtta
    26581 aaatttatct aacaattaaa cttaatccat tgctttaata cgtttaatgc gtttaatcgt
    26641 atttaaaata taacgccata atggcacaag taataggatc atccacgctg gatgccattt
    26701 acccgttacc gcaccaacta cgacaaaggc gaggataatt aaagcatcaa cggaaaataa
    26761 ggcgattttc ttttctaaaa tggctgtcac taatgaatca acgacaggaa ttgataagaa
    26821 aataacccaa tgtggatgcc atttatcaag gaaaaaaacc attgcgaggt aagcaataac
    26881 gacaagcgtc acatatgtcg acattactaa ttttctaatt tcaaatttct tgcgtcgact
    26941 cattccctct ttattttctt catcaatcac ttcaacttta acaaattcag ctgtatcact
    27001 cataaatgtg ttcctctctt tttataaatc taggcgatat atcgcgaggg gctctatacc
    27061 tttataataa tacatataat tttcaggtgg tgtgacaatt tctaataact ttgcacccat
    27121 tttctcaaaa gtgcgtaaat aacccttgct cgaacttggc gcactaaaga taacgtattc
    27181 catcttgtgg tactttgcga caacaagaat tgcgtttaat gctttaaccg caagattttg
    27241 tccgcgatac gctccgctaa cttgataaac aaagttccca ctgtagtaag aattaaaatt
    27301 atggccgatg cgcactaaga tgttcccgac taaggtaccg tcatttaaat gaataccata
    27361 tttataataa ggtaaaccac caattaagcc actatttttc tcagtaatac ttaaatagat
    27421 gtcttcacca ataatcttgt caaagtcttg attaaactgc atatttatca ttcctcctgt
    27481 ttcattataa acggaatgat tatttttctt cgtgcttttg cttatccgca ctttcagtca
    27541 tatcaaaata acgttctgga tgattagtga taaaagtatt aaacattgtt ttaagcgttt
    27601 cttcagcctt tttatgatct tttaagaagc gcacaacata aagttcacta tctttcgttg
    27661 caatcactaa attacggcgc atattcccaa cagagctaaa attactaaac gctacttctt
    27721 ttgtcttttt cttacttaaa tgtaagtaag cgccttcttc atcaacactg agtactactt
    27781 taggacgaat gaggtaaata attaaaaaca atgcttcgcc accaaataaa ataaggaaaa
    27841 ggaatgatag taaaacaata tcaccacttg tgctaccagg gtttttaacg atcatcacga
    27901 cccacgaaat aaacaaagta attgttacaa gtaaaacccc gacataataa aataagtatc
    27961 gtttagattt ttcaaaagta atcataaatc aagtgttttt tagtaaccgg tacctttttt
    28021 accagtaaag aaagcaacac cattactcgt gccaattcga ttagcgccag ctttgaccat
    28081 cgcttcaaga tcttccatag tacggacgcc gccactagct ttaacgccca tatcactccc
    28141 aacagtgtca cgcattaatt taacatcggc tactgtggct ccgccggtac taaaaccagt
    28201 actagttttc acaaaatcag caccagcttt acggcttaaa agacacgctc taactttttc
    28261 gtcatcagtt aataaacatg tttctataat aactttaaga atgacccaat caggaagtgc
    28321 ttcacgaatc gcccggattt cagtaaagac ttctaaatct aagccagctt taagtttacc
    28381 gacattaatt accatatcaa cttcgtcagc accttcttcg accgcaattt gcgcttcaag
    28441 aactttcgct tcagtgcttg ttgcaccaag tgggaagcca acaacagtac acacacggac
    28501 atcactttcc gctaaaagca tactcgcaag tggaacgtac cccggattta cgcatacact
    28561 cataaaatcg tattcaagcg cttcttgaca aagtttttcg atttgtgcgg gtgaagcatc
    28621 cgctttcagt aaagtatgat caattagttt attctttttc atattaccct ccaaatgata
    28681 ttttaccatt ttagctaata tttaattact tttaatatat aaaacaccta aaacaactat
    28741 ttttctactt tataagaagt tttgtaaaag gattttcaaa agtgctaaaa gttcaagagt
    28801 tttgtaaatg gattttcaaa actttggatt atttaaaata aaaaaggaag tttccttcct
    28861 catttattta gcggttaata cctatttctt tgctttgcct ttttttggtt cttctttagt
    28921 aatcacgtct ttaccagcat agtggtgaca ttctggacaa acatgatgcg cccgaatgag
    28981 tgttccacag tttgggcaag tggtaagtcc agtcacgttt aacttaaagt gagtccggcg
    29041 taagcgtttg cgggttttac taccacgtcg ttgttgcaca gccataaact tatcctcctt
    29101 cttgtgacta gggttattat agcaaaagaa aaagtagtgt caagaaattt agacatttta
    29161 tagtatttat tttattatat tgattatatg ctcataattt agtgcattat ttttatacag
    29221 tgtctagatt tttttactaa tattcgggtc ataaataata gtaattactt catgcttaat
    29281 atcgctatcg cttttaactc taactgctaa ataattagtg gtatgcccaa catatacttc
    29341 ttctttatcg tcataagttt caattaaaac ttgcattttt tcgccgacaa atctttgtgc
    29401 ataacttcga tataagtctt cacttaagtt tattagtgta cgcacccgtt cttttttaac
    29461 tggaccaagc acttgctctt taaaactagc ggcaggcgtt ctcgtccgcg cactataggg
    29521 gaagacatgt agctgcgtga agtttacttt tttaataaac gcgactgttt cattaaataa
    29581 cttttcactt tctccaggaa agccaacgat aacatcagtg gtaatagcaa tattaggtac
    29641 tttttctcta atattttcga ttttatgtat gaattcactc gtggtataat gccgattcat
    29701 cctgcggagc acttcatcac ttccactttg cagcgggata tggagatggc gtgctatacg
    29761 cttgtctttt agcattaaac gaaggaaatc gtcatcaatt tcatttgctt caatacttga
    29821 tactcgtaat ttatgtaatg atggacaagc ttctaaaatc atctccacaa tattactaaa
    29881 tttaatattc cctaagtctt taccgtaggc tcctgtatgg attcccgtta aaacaatttc
    29941 tttataccct cttcttacta agtctttagc ctccttaaca atgtcacgca catcgcgact
    30001 tcgcatcttc cctcttgcaa gaggaataat acaatacgta caaaaagcgt cgcacccgtc
    30061 ccctattttt aaatacgcgc gcgcctgtgg tgttacactc gtagcgccta actcttcata
    30121 gttaaatgtg cgtggctctt cttcaaccgc aattattttt tgaccatgct ctttaaattc
    30181 ttcaatataa tcgacaattt tacttcggtt actagtgccg actaaaatat caatttccgg
    30241 aatggtattg attaaatctt tgttaagctg tgcgtagcat cccattacaa ctaaaatagc
    30301 atcaggataa tttttcgcga gtttacggat atgttgtctg cttttttgat cagcgttact
    30361 tgtgacgctg catgtattaa tgatagcaac atcaactttg tcaccttgat gagcaagttt
    30421 gtaaccttgt gcttttaaag tttcttcaat actaaaaact tcgtaagcat ttactttaca
    30481 accaagtgcg acactagtaa aggtcttcat ccaattatct tccgtctttt taagttatta
    30541 atatatttag tgtataactt atgactaatt aacttttctt tcacaagagc attaataaaa
    30601 gcaatgcgat taccagcgta atattcagta agtgccggtt taattcgttc taatacatta
    30661 cggaaatcag ggcgcttaat atgtttatca aataaacaaa tcttcaaatc aagtcgtcct
    30721 tgatcattaa taatataaat cgcatgaaag tcataatgat aataagcgcg cgtatgaata
    30781 tcttcaccaa ctaacacaag actagtaagt tgcgattcaa ataaactaaa attatacgct
    30841 tcaaataaat ctatcgcgtc atgggttaaa tttagtttac ttcctaaatt attcccaatt
    30901 aaaatgttat tgctaacact aaaaagcggg caatatatgg gtacattatt ataggtaaaa
    30961 agtgctttta ctggtaattc tgtatcaggc ttaacttcat tattccagta attagtgagt
    31021 gcttcataat ttaagagtgc actttgaata atattcgcac agattaaatc aaagtcatct
    31081 ttactattaa taaaaggaat aagttcagga acttgcgtta acagtgcttc atgatcttgg
    31141 aaaaataaat attcacgact cggtgtaata aggtgccctc ggcgattttc ctgtaataac
    31201 tcataaattt tcatggtgcg ctcctttttg ataatgatgt aacacactta aagcatatat
    31261 cgctgcggtt tcacttctaa gtatattatc gccaaaagta atgacattaa agcctttagt
    31321 actcgctaaa gtaacttccg cttcactaaa accgccttct ggaccgacta aaatgagtaa
    31381 attatcgcct aaatcttgtt gcaatactgc tttaagcggt aaattagcgg tttcatgagc
    31441 aataagctta gtgtcaaact ctaaatctaa aacttcttta aatgctaagg gttcagtaag
    31501 cgttggcaga tagttacgat tagcttgcat tgctgcactt ttaataatct tattaaagcg
    31561 tgctaaacga ctttctaagc gcgttgcagg tattttaccg atactacgct cactatcaat
    31621 taaaatgatg tgactaacac caatttccac tgctttttgg atcactaatt caagtttatc
    31681 gccttttggt aaggcgtaac ctagtgttaa gcgcggtaag ggctctaacg gtaactttct
    31741 tttttctaaa acattaagta taaacggatt tacactttca taagtgcata agtaaagact
    31801 ttgtcctgcc actacttcaa aagtttcatc ctttttaagc cttaatactt tcgttaaatg
    31861 aaaaatatca tcatcactta agactacttc attatttcta actctgccaa aaaacctgta
    31921 actcataact ttaaatccac atatatttta gcattaacgc ataatgaaaa aagcactgaa
    31981 cgctaaaata ccaacgacta aacaataaat cccaaaataa attaagtgtt ccacctttaa
    32041 ctttttcatc acaaatagtc ccgttagaaa agtcgcacca aaagtaaaga taaacgctaa
    32101 gatgtaaccc gtccaattaa tttcttcact aatatgtact aagtcaactg cgcctaacac
    32161 acttgtcggt acactaataa taatataagc aaggaaacta aatcttaata tactttttag
    32221 tgacacacga cttgcgcgac caccaatgat ggtggagccg ctccgcgaaa taccagggaa
    32281 aacggcaaac gcttgggtaa agcccacgac tattgcgtct ttccacgtta tatcatcatg
    32341 ttcctcaatt aaattacgcg taaagaaaat agtaagtaaa agcgcaccag taataaataa
    32401 agcaatacca acagttaata gattcttaat catccccatc tcatctttaa aaataagtcc
    32461 gacaaccccg agcgggatca ctgcaactaa aagttttaaa acatagaaaa aggtgtcttt
    32521 atactcttca cgcgcttctt tcttaaagat atacagaaaa aatcctttaa ttaattccca
    32581 tatatctttt gcaaataaca caaataaggc taaaaaactc gcagtatttg taaacatttc
    32641 aaaaactaac cctggttgtt ctagcttaag taattcttga aagatgacta gatggccact
    32701 agagctaatt gggaagattt ccgtgacccc ttgaataatc ccaagaataa tatatttaag
    32761 aatttccata actttcactc actttgctta ttactttgta attatagcct attcttcatt
    32821 atttagatgt taaaaaaggt taggacgtgc ttatcctaac ctttggcttc aagtttatta
    32881 actattaggc atttatccaa attgctggaa gaatacccat tgctgcatca gtgacattta
    32941 tagcggcaag ggcgccgtta taatttaccc gcgaagcact tgtctcacta gaatatgggg
    33001 aacgtgtcca ccaataagct ttgtcataat ctttactaac ggtctgtgat aatgcataat
    33061 cacttggata acgcactcgc gaagcatcat caacaaaacc ataatgtccg ttcaaatcta
    33121 agtaacttag cagaaacact ttatcaagag tatttggaca atcgaagtta tgcacattat
    33181 taattgttgt cgctttacta ttatcaacag ttgtcaccgc aattttattt tgttcagttg
    33241 tagtaaaagc agcatttaaa aatgaagaat ttaaccatga cctaatatgt gaatgttcat
    33301 agttattagc gtacaccgtt tccccatcaa tatttcgtct tgtcttagcg taaaaatagt
    33361 cttgagcgtc aagtacagtt tgcgctaaaa ggaaagcttt accagcatca atatcaagca
    33421 ctttccacgt tattggttca tatttaaacc agtaaacaat acgttttgtg taaccatggt
    33481 cttgttgatt aaaacatcca gcagtgctat ccgtataagt atagcggtac tctgtgtaat
    33541 agacaccgcg atattcctcg ccctcattta ctaaatcgat ataccacata aaggaagtca
    33601 ccgcaccatt atagtaataa aggtaatcag tccacgcttt cgcgttttcg gcagttggta
    33661 atccttcact tccgcctgct aaattgttta gtgtagtaat aagcgttaca tccgttactc
    33721 ttgtttgcgg gtaagcacca aatttcattt cttccccttc tgcgacaaaa tcagcggata
    33781 acacaagatt attacttggc atagcgaagg tataagttgc agagcttgac actaaattat
    33841 tactagcgtc aaaccacccc gcaaagaagt atggagcaag tgcagttgcc gttactgtca
    33901 cattagttcc agcgcggaat aagccctcac tcgtgactgt accacgcgca tcatttttac
    33961 taagcacact aagtgtatga tagtgatcaa cacagtcaaa ttcaattttg cctaagttaa
    34021 aggaggaatc tgcaagcgca taaatcccta tatatttagg cttataacca ttaaagtccg
    34081 tgctaaagtt cgttggtgaa ctagcgttaa tcactacttt acgcgaagca tcaaatgaca
    34141 ttgtgttgga ataaagaatt tcaacattta cagtgtcatc gcgttttgtt aactctagat
    34201 tcgtaatccc gttaatcggg tcactattga caatataacc acccgtttta attctttgcc
    34261 agtgagtcga agggtttgtt aaatgcttgt aagaaacagc aatattgttg cctagttcgg
    34321 ttgtaagcac tcctgttccc gtataaccat caggattttc tgatccttcc gctattttgt
    34381 tcttctcagt accaagaaca atggcgtacg gtgttggttc accgcgcgca ctagagccgt
    34441 gattactccc aagactaatc gcgctaataa gtagtgccga taacgcaaaa aaggctaagg
    34501 tcgccttact aagcaactat ttgtttttca tagattattt ccttccgacc aaatgaacat
    34561 atgttcataa atttagttta acaccataaa atcaattaaa agttttaatt tcatgaccta
    34621 aattagtaaa aagaaggatt aaaacttcac ttaaaataaa ttaattgcat atattcgtaa
    34681 taatttagtt gattattaac taaagcaaaa attcaatttt ttgtgataat gttttttata
    34741 caaaaaaagg ctagggattt attcctaacc tctttcatat aattttaata aattttagcg
    34801 tttaaattta gatttaaatt tctcaaagaa tgtttctttc tttggctccg ctttcttgaa
    34861 gtcttcaagc gccacttttt gatctttact taaattagtc ggtgttttca cttctacttc
    34921 taagtattgg tttccaacat taccgcgtac gtctttaacc ccttggccac ttaagcgtaa
    34981 agtagcgcca ggttgcgtac ccgctggtat tttaacttcg acattgccat aaaccgttgg
    35041 gacttcaagt gtaaccccaa gcgctgcatc gacaaagcta atggggattt gtaaataaat
    35101 atcgttacca acgcgcttaa aactattatg cggtttaatg acaatttcaa catgtaggtc
    35161 accattagga ccaccgttaa gtccgcgttc acccttgcct tgaatccgta gtttttgacc
    35221 ttcaccaatg ccggctggga tgttaagtaa ttgcttttct tttacacggg tatagcctgc
    35281 accattacat gatttacacg gtttactaat tgttttcccg ctgccaccac aagatgtaca
    35341 cgctctttga ctttgcacac ggccgaaaat actttcttgg acaaaggttt caaccccgct
    35401 gccattacat ttactacatg tatggatatc gtttgcgttt tcagcgcctt taccttgaca
    35461 cctttcacac atcttgtcat aagtgtgcat aaattcaatc tttgtgccat taatcgcatc
    35521 cataaagtta atgcgtaccc gcgttaataa gtcgttccct ctagcgggtc cagtttgccg
    35581 actagcgcgt cgtccgccgc caaagaagcc ttcaaaaata tcgccaaagt caaacccgaa
    35641 gccactgcca ctaaagccac caccaaaagg attacctccg ttttgatcaa aggcagcgtg
    35701 accaaattgg tcatatgtag cgcgtttttg atcgtcatag aggacgtcgt aagcttcttg
    35761 tacttcttta aacttctctt ctgcatcctt ttctttattc acatcaggat gatacttctt
    35821 cgcaagttta cgataagcac ttttgatttc atctttcgaa gcacttttct ttattcctaa
    35881 tacttcataa taatcacgtt tagccatcgg cagctccttt tacatacaga aaccccggat
    35941 ggcggggttc ctgaaagttg tttcttaatt taaattagtc ttttttatca gtgaaatcag
    36001 cgtcaatgat gtcatcatct tttggtgttt cagtagcgct agtttcacct tcggcttggc
    36061 cttgttgcat attttgcatc atttgtgcca tttgttcgag ttcacttaaa cgtttttcaa
    36121 gcgtttcgta atcttctttt tcaatcgctt cttttagttc agtgacgagt ttttcacttg
    36181 cttctttttg attagcgtca actttatcgc caccttcttc taaactatgt tcgatttcat
    36241 taatatattg ttcagcgcgg tttttaagtt caacattttt cttgcgcttt tcatcagctt
    36301 ctttatgctc ttcagcgtct tttaccatgc ggtcaatttc ttctttagag agtccgctgc
    36361 tattagtaat cgtaatatgt tgttcttttt gcgtgtctaa gtctttggcg gttactttaa
    36421 caataccgtt aacatcaatg tcaaatgtta cttcaatccg tggttgaccg cgacgcgctg
    36481 gtttaatgcc gtcaaggcgg aagtgaccta ataatttatt atcacgcgcc attggtcttt
    36541 caccttggta gacaacaatg tccaccgatg gttgattgtc ggctgccgta ctaaagattt
    36601 gtgatttagt cgtcgggata gtcgtattcc gtgtaataag cggagtcatt acttcaccca
    36661 ttgtttcaat acctaaggtt aacggagtaa cgtcaagtaa gacaacgtct ttaacatctc
    36721 cagcaataac tgcaccttga atggccgctc caattgagac tgcttcatca ggattaacag
    36781 taaggttagg tgctttacct gtcattttct ttactaactc ttggacagca ggcattcttg
    36841 tactaccgcc aattaagatt acttggttaa ggtcacttgc tttaacttta gcgtcactta
    36901 gtgcccggcg aattggttct tcgactctcg ctaataagtg ttttgttaaa tcaaggaact
    36961 tcgctcttgt taatttacct tcgtaactaa ttggtccgct tgcgcccata ccaataaagg
    37021 gtaaactaat tgttgtttca agcatttgac ttaagtcaat ctttgctttt tcagccgctt
    37081 cacggaagcg ttgcatagcc attttgtcat ttaactttaa gccatgttct ctttcaattt
    37141 cttgcttaat ccattcgaca atctccgcgt cccagtcgtc cccacctaag tggttatctc
    37201 cagcggtcgc gataacttca aaggtaccat caccaatctc taagattgaa acgtcaaatg
    37261 ttccaccacc taagtcaaac actaagatct tttcatcgtg ttctttatct aaaccgtacg
    37321 caagtgctgc tgcggtcggt tcgttaataa tccgcacaac ttcaaggccg gcaatttcac
    37381 cagcgacttt tgttgcttgc cgttgcgcat cattaaagta agcaggaacg gtaataacag
    37441 ctttttgaat actttgccct aaattctttt cggcataatc tttcatataa ccaagaattc
    37501 tcgcgcttat ttcttctggt gtaaattctt tctttaacgc attaatttta aactttttat
    37561 ttgtcccgat gtgacgctta acactcatca cagtatcagg attagtaacc gcttgacgtt
    37621 tggccgcttc accattaact tgttcaccat caggtttaaa ggcgacgaca cttggggtgg
    37681 tcggtttgcc ttctggggtt ggaataactt taatttggcc gtcagcttgg cgatagctta
    37741 cgacactatt agttgttcct aagtcaattc caataataat ttctttcttt gccataatat
    37801 atatctcttc tcctttttat taatttaagt cctcaggatt aacttctggg acattatcat
    37861 cattttttgg ttcttcttta atattcgttg acactataac ttgcgcgggg cgaattaagc
    37921 gatcaatgaa tttaaatgtg ttattgcgca caccaaggac ggtgtttggt tcaacttctt
    37981 cactgtattt aacttccaca gcgtgcatcg agttttcatc gaatttatcg ccgacattca
    38041 caactacttc ctctaggcct tcactagtta aggcatcaag tagttgtccg tagacgaagc
    38101 ggaagcccgt taagtagtta ttaagcactg gatcttcttg ttcttcgcga atgacatgat
    38161 tgaagatatc aagcgctggt aagagttttt caataaatgg tacggcacgg tatttaatgt
    38221 aatattgttg atctttcgca ttttgtttac gcgcattatc catatcagcg agtacgcgca
    38281 tatatttgtt atgccactca gcactttcag cttttagttt ttcaatttca gcttcgagtt
    38341 tttgtatttt cgcgagatgg cgtttttttg gcgctacatc tttttccaca tgtttctttt
    38401 catcactcat cattaatctc tctttctatc tcgaagtact tttcgagctc atcagttacg
    38461 tattctaacg cccataagac tttactgtag tccatccgtt ttggaccaac taggacaatt
    38521 tgtccgacct ggtcgcctgg gacatggaca ttggcgctta tgaaggttaa gtcttcatct
    38581 ttgcccccga tattcacact taagtcaccg tttcccgtga taagttcacg tattttctct
    38641 ggatcatcaa ggattgatat gagtttacgg agcttttcac tatcattaat gaattctggt
    38701 tgctcaagta agctttcttt accgtataag ctaagccggt cacgggcgta ccttataaat
    38761 gcatcagtga aggcatttgc taagacttct tgttcaacaa tgtaatcggt taagagtggt
    38821 tgtaagagtt ccatctttgc cgatacttca ctaattcttg taccgactag gcgttcattg
    38881 aagatattag cgactttgct aatttcttga atacttgttg aaggcgggac gatgaaggta
    38941 ttatgttcga catagccgcg attagtgaca aatattgccg tccccatgtt tttatctaac
    39001 gcgacaactt gaatactttg tagtgtttca ccttttttct cgggaccaag tgcgacactg
    39061 acaagatttg tcatttgtga gagaatttca catgtttgat taattacttg ttcaacgctt
    39121 ttcgtcttct cttccacaag cgtcgcaatg tgatgtcgga agttatcatt tacttcatct
    39181 tcacgtaagt tagcaacata gaattcatag cctttcttac ttggtacacg gccagcacta
    39241 gcgtgtggct tttcaagaaa gcccattcct tcaagggcat tcatttccgc tcgaaccgtt
    39301 gcactgctat agtttaagtt ataattatcg attaatgact ttgaaccaac aggttgggca
    39361 gtcttaataa ataactcaac agtatacttt aagattaact ctcttcgtct gtatctcata
    39421 actcctcctt agcacttgca actttcgatt gctaaatata ttatactaaa gaaaatagca
    39481 ctgtcaacat ttaagtgcta aatttcgtaa atttttacta attacttcgt aaaaagccaa
    39541 aaattaggct atttttttgt taaatttgct tctttttggt tttttaatcg catgtttttc
    39601 attaaaattt aagagccgta aaccgtttaa tatcactaaa atagtactac cttcatggat
    39661 cgctactgtt aatcccattg gcaatgtcac cacgaaatta agcgcaatta ataatagcac
    39721 cacactgagc gctagcacaa tattttgaat aacaatgcgt ttaagttttc gtgaaatagc
    39781 gtgcattaat gataactttt ggaaatcatt gttgacaagc accccatcag cgacatcaat
    39841 tgccacatca gtgccatccc ccattgcaat cccgattgtg gcttcaacaa gcgcgggcgc
    39901 atcattaatc ccgtctccca ccatcgctac tccgccgtat tcctttttta gttctttaac
    39961 aatatcaagc ttttgttcag gtaatacatt cgcgtatatt cgcgtaatgc ctaactcttt
    40021 accaatagct tttgccgtta attcattatc tccagtaatc attactgtat taatttcttg
    40081 ttttctaaag tattcaagcc catcaaaggc acaacattta atcatatcta aaagcgctat
    40141 aagtaagatt ggtttcccgt tttttgcaat aaagacgact gttttacctt gaactgccaa
    40201 ctctttagcg cgattagcgc agcgctctgg taaattaaat aatgctggtt tacctacaag
    40261 atactcgtta ccgtcataca ttgcgcgtaa tccactgcca attttatgtt caacttctaa
    40321 atcaactgtt tttataggag taaaatgact aacaatcgct gtcgctaatg ggtgattact
    40381 tttcttttcc atactgacaa ggaaaacttt tccttcttct gcttcttctg cacttactcc
    40441 tggcgggata ttaacatcag taacactcgg tttcccttcc gttaaagtac cagttttatc
    40501 aaaagcaact gcttctacat ccgcaaatag agctaaggaa gaagcacctt taaaaagcac
    40561 accgcgtctt gctaaaagac ttaaagcact tagacttgcc gggatatcac taacagctag
    40621 ggcacacggc gaagcaccga taaaaagaat aattgtgcgt ttaagcgcaa gcgtcatccc
    40681 gttagcgcta ataacttcat tccaccctaa aacataatag ccaaataaat aaaatagtgg
    40741 agcaattaac atcacaatag ttacataaat tggttcaaat ttcttaataa atgtcgcagt
    40801 tttagttaaa ttagtttgcg tttcactcac aacccttaag attttcgcta ataccgtttc
    40861 atcactattt ttggtcacta ccatcgtaaa agtactgttg ccattaatac taccaccaaa
    40921 gacttcatca ccttcatgct tctcttttgg catactttca cccgtaatca tcgcttcatt
    40981 aattgcggca ctaccactaa ctatcgtgcc atctgtcggg atttgatcac catttaacac
    41041 aagtactttg tcaccaactt ttagtgactc aacacttact tcttgttgtg ttccgtcttg
    41101 aagtaaaagc ctagcggttg tcggtgttat ctttaaaaga ttcgtaattt ctttggcact
    41161 ttttgcttca gcatattctt caagaaaata cgcgcccgcg aatattaata taagtaaagc
    41221 agcgtcgcca taatcaccaa taataatcgc cccaatcgcc cctagtgcca ttaagatatg
    41281 aatatttgga cgaaatcttt tacttttaat actctcttta accgtttcga ctagtccttc
    41341 actaataact ttgtaacccg caagaataag cgctattaag tttaatatca ttaccgcttt
    41401 atcacttaaa aaactcacac cggggataaa tgctaaaagt gcaaataaaa agagcaatag
    41461 cccgataaaa taagcataga gaccaaactc gtgaaatatt ttttgaaatt tactttgttt
    41521 attatcttgt atcatatttt tactccctaa tatggtcacg tgcttgttta ataatttcat
    41581 aaatatgcgg gtcaagaatt tcataaatcc gctcttggcc agcttttgaa tacgtgacta
    41641 cgcgatggtc ataaagtgtc cgtaattgat gactaatcgc actttgcgtc atatttaatg
    41701 cattagcgat ttcagtgaca ttctttgcgc tttcttctaa gagtaaaata atacgcatac
    41761 gcgtatcatc tcctaagact ttaaagaagt ctcgtaatct ttcaatatcc acgactttat
    41821 ccataatttt ctcctaacac ttgaatattt gagcaagtgt tcatatacat tataaaaaat
    41881 atatctaata tatgtaaaga tatttacatt attaatgtta aaacgacttg atcaaggagc
    41941 ataatccctt catctgtcgc atatactttg tcatcagtga catttaaaag tcctctttct
    42001 atgaggtttt ttaccttagt gccatacttt tctaaaaaat caaagccgac acgagtttta
    42061 aagtcttgta aactaaaacc ttgtgcgagt cgtaagttaa gcattaagta ctcttcaaca
    42121 tattcttcgc ttgttatttc gtcgtaattt atgatacttt cgccttttaa atacttagtt
    42181 aaaccgccac ttattaagta gcgtttacca tcaatatacc cactagcgcc aaggccaatc
    42241 ccgtaataat tacggttttc ccaatatagt ttattgtgga tactttcata tccctttaac
    42301 gcaaaattac taacttcata gcgtatataa ttattatttc ttaatgtttt taaaataaag
    42361 tcatacatct cgcgtaaagt gtcgtcactt ggaacttccc actttttaag tgcagccatc
    42421 gtgccttttt caatcgttaa gctataggta gaaatatgtg gtgctcctag ctttatgatg
    42481 ttcattaagt cattttctaa gtcgctaaga gtttggccag gtaatccgta aattaaatca
    42541 acactaaagt ttgtaaataa cgccttttta acaagcgcaa tctttgtttt aatcatcgta
    42601 taagtatgat gacgatttaa ttttttaagc atatagtcat cagtggtttg cactccaata
    42661 ctcagacgat taacaccgta acttcgcatt aatttaagtt taggcaaggt taaactttcg
    42721 acattagcct cgactgtata ctcttttaca ttagccgtat aaggctttaa taattctaaa
    42781 agattacgta actcataata gcttaaagac gaaggcgtcc cgcccccgat ataaagcgta
    42841 cttaagtcac gactaacatt aaaaaaactt aagtcatgct taagcttttc taagtaagat
    42901 gcaacccact ttttttgata aattacttta acaaaatcgc aataatggca tagtgaagtg
    42961 caaaaaggaa tgtgtacata aagtccacgt ttagtcattt ttcgtttctt tatctacttt
    43021 ttcaaattcc gctaagattt tttcatcagt aattggttca agtactctat ctaattcttg
    43081 ttgaacagcg acttgttcat taacgcgacc acctttatcc tttaaaccag gaattaagcg
    43141 gcgaataata aaggtaataa gcgcaattaa gccaaaaaat agtagtaaaa ataacagaat
    43201 taatcctggt gaatcaatcg caagtgttgg taaataaatc ataatcatca tcctatgctt
    43261 tccgtgttgg tgtataaata aatttgtttt taaatttttc tttgcggtaa tatccgctgt
    43321 tagcgaggtg ttccattgat gtccgcgctg tttcatacgc acagcctgtc acttctttaa
    43381 agtcattaat tgagtaatat ttacccatag tacagtgtct agcgtagaaa taagcttgag
    43441 cgcgacttaa acttggatcg ctttcacgaa tatgtcgctc taagcgtaat atatcatttt
    43501 cgctaagttc tggtggtaat tgtgtaatcc ctaaattggc ctcttctggt gtaactttta
    43561 cgccttcttc gcgcggcgga agtggaagcg ggattgcgga tttttttggt gtataaactg
    43621 gtactggttc agatttaaaa gtttcttcct tctccgctac ttcggcttgg gcatgttcaa
    43681 aaagatcagt tggtggtgta gcactcacac tttcttcttg cgtttcttct tcaaatggtg
    43741 ggagcacatg cttttctgct tcaagttcat gcgtcacgac aaacacaatc cgttcttcaa
    43801 ttgtttgcag tgattcatta agcacattaa taagtaaatc aataaaataa gttaaatcag
    43861 cacttttctt tacttcggcg aaaacatcat cacgcgcatc acggtatctt tctaagataa
    43921 gttcaaagtt aagataaggc gcaataagcg cgagttctct ttctgcaagg aaggcttttg
    43981 ccattaaaat ggcaataatt tcactatgag cttcaaaagg tttaatataa tcaagataga
    44041 aaataatcgc tgcactttgc acaatggttg gtaatgtact tgtggccata aatgcgtaaa
    44101 aatcagcgat tgcatcacta atttgattga cgggaacatg cggatgaaca tgattaatga
    44161 cggagacttg acgccccgtt ttaatttcgg tagtgcgata aatatagtta cttagcggcg
    44221 gaacattaaa taggcgccca acatcagcta aaagtgcttc agtaaaatca agattagcgc
    44281 tttttgctac tagttctaac ccgcgataat aatcatttaa aaacatctcc cacgcattta
    44341 aaacactaat attgtgatta atgagttgat taacaatccc atcatgaaaa ttaatttcat
    44401 aactagcggc gattgttttt aaaatatcac gagttaataa ggttttaagt gccttttgat
    44461 gattagcgtc aacataaact tcttgaattc gtgcttgaat acgcgagatt cgccggtcaa
    44521 gttgatttaa gcgatcatta attttggggg ttaatacaac gcgtaaacga ttacgttcaa
    44581 ccgtgcgtaa agggatggtc acactgaaca tattgcgata ttgtaatact tctttccaaa
    44641 tatcatcaat cagtgttgtg ccaagtgccc tactaacttg attcatcgtt agatagcgtt
    44701 cattactaaa gcgttctcct tggttagtgc tcatacattt cttcctctat taaagtgtac
    44761 cataattgat gtttgttttg acccctaaaa ctataaaact actaatattt tactaattat
    44821 catcatcaat acttaaaact gacatgaagg cttcttgtgg aatctccact gaaccgacga
    44881 gtttcatccg ttttttccct tctttttgct tttctaaaag cttgcgtttc cgactaatat
    44941 caccaccata acacttcgct aagacatctt tacgcactgc tttaatatcg gttcttgcaa
    45001 taatcttatt attgatagct gcttgaatcg gtacttcaaa ttgttgtcgt ggaatgatgt
    45061 tttttaaggc tcgacacatg gcaacaccgc ggttataagc agtcgggcga tacacaatta
    45121 gacttaaagc atcaactggt tgaccattaa gtaaaatatc cattttcgct aagttactag
    45181 ctttatagcc gctaagacta taatcaagtg tcgcatagcc ctttgaataa ctttttaatt
    45241 tatcaaagaa attgtaaatt acttcagata agggaatctc ataagtaact tgcattcgcg
    45301 tatcatcaag ataagataaa tcgacataaa taccgcgctt atcttggcat aattccataa
    45361 tgggaccgac gtattcttgt ggtgtcataa tttgtgcttt aacatatggt tcttcgatac
    45421 ggtcaacaag tgtaaaatct ggtaatttac ttgggttatc tatttttaac atcgtcccat
    45481 tagtgagata aacatgataa ataacacttg gcgctgttaa gattaaattt aagtcgtgtt
    45541 cccgttcaag ccgttcttga attacatcca tatgtaaaag ccctaaaaat ccgcagcgga
    45601 aaccaaaacc taaggcaaca ctagtttctg gctcatattt taaggctgcg tcatttagtg
    45661 aaagtttctc taaagcgtct ttaagatcac cataatcctt tgaatcaact ggatagagac
    45721 cgcaatatac catcggcgta atttcgcggt aacctggtaa agcttccttt gctttattat
    45781 taactaaagt aacagtgtcc cccgtactaa tatctttgat atctttaatt tgcgcggcta
    45841 agtaccctac ttcaccagcg cgtaatacat cacgctttac ttcttgcggt gttctaatcc
    45901 ctaattcagt gactaaatac tttctattcg tcgccatcat taacacttca tcacctacac
    45961 gcacttcccc ttctttaacg cgcatcataa taatgacacc gcgataaggg tcataataac
    46021 tatcaaaaat aagtgcttta agtggtgctt taacatttcc gcttggtgca ggcacatatt
    46081 taacaattgc ttcaagtact tcttcaatat taaaacctgt tttagcgctg acgccaataa
    46141 ctttagaggg atcaagacca atccgtttgt aaatttcttc acgcacttct tccactcttg
    46201 cacttggtaa atcaacttta ttaatcactg ggataatctc taaatcatta tcaattgcta
    46261 aatacatatt cgctagtgtc tgtgcttcaa caccttgggt cgcgtcaaca actaaaatcg
    46321 ctccttcgca cgccgctaaa gaacgactaa cttcataagt aaaatcaaca tgccctggcg
    46381 tatcaattaa atttaaacga tatgttttac cgtctttcgc caaataatca agggcaacag
    46441 cattaagttt aatcgtaatc ccgcgttctc tttctaattc taaagaatca agcacttgcg
    46501 ctttcatttc acgcttttca agtgtgcctg ttgtctcaat taagcgatct gcaagggtcg
    46561 atttaccatg atcaatatgg gcaataattg agaagttacg gattaaatct ttgttgtaat
    46621 tcataatact tactctcgag atagtattat accaattttt attaaaattg actaatgaga
    46681 aaagactacg ttaaaggaaa aggttaaatt tacatccttc ttgtcttcat tataatgttt
    46741 at
//
LOCUS       DAOSTA010000004        46520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_888_length_46520_cov_14.085850, whole genome shotgun sequence.
ACCESSION   DAOSTA010000004 DAOSTA010000000
VERSION     DAOSTA010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 46520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46520
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_888_length_46520_cov_14.085850"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLU08_00870"
     CDS             <1..794
                     /locus_tag="PLU08_00870"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779933.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOQ70296.1"
                     /translation="TVRLLDPPLHEFLPTEEAQIVALAKELKVDVTVLRERIESLSEF
                     NPMMGHRGVRLAVSYPEIAVMQTTAIISAALRAQKDTGAAIHPEIMIPLVLDVKEYKY
                     VEEYVRKTADALIKKSGQKLHYLVGTMIEIPRATLLADQIAKEAEFFSFGTNDLTQMT
                     FGFSRDDATKFIPDYTEARIFEQDPFQTLDTEGVGQLVRLGVERGRKGNPDVHLGICG
                     EHGGDPNSVEFFDSVGLDYVSCSPYRVPIARLAAAQAAIKHKKAK"
     gene            complement(899..2287)
                     /gene="glmU"
                     /locus_tag="PLU08_00875"
     CDS             complement(899..2287)
                     /gene="glmU"
                     /locus_tag="PLU08_00875"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOQ70297.1"
                     /translation="MKRNAIILAAGKGTRMKSLNQEISKVGFEILGKPLINWVLDAAK
                     PHVTGRTVVIVGFGGEHTSKLVEGKAEIAWQHEQKGTGHAIIQTAPLLEKEKGDTLIL
                     SGDVPLITEHSISGLLNLHTKEKNDLTILTAKPDNPFGYGRVLRAPNGEVTRIVEQKD
                     LTGNEGDISEVNAGMYVVNNQKLFQELKNLKPNNKQGELYLTDIIGLFKERGYKVGAF
                     VLSDPDEMLGTNDRAQLAEATELLKKRINKAHMLNGVTLEDPNNTYIGPDVTIGQDTI
                     ISPNTTILGNSTIGVANEIGPNTYLKNMKIGDYNSIIYSYLVDSEICNNVTIGPFARM
                     RQKALVKDGSHIGNFVELKNTTFHEGVKAGHLAYMGDAELGAHTNVGAGAITANYDGV
                     NKSKTKVGENVFIGTNTTLVAPLQIGEGSYLAAASIINKDVPKDTLAIARSRQENKEG
                     YAKIIREKQKKK"
     gene            complement(2297..3148)
                     /locus_tag="PLU08_00880"
     CDS             complement(2297..3148)
                     /locus_tag="PLU08_00880"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70298.1"
                     /translation="MIVTANARLSISVETTLNPLTNALEIKSLVLPIEFSDTITIDVF
                     NTAFMDIVHYTNKALHVKEKRIIDEALLKMREQFGYKQNFSVKIHKNIPTGHGLGSGA
                     SDATAVVKAVAHLAKLKPTPEQYYNLAVKIGKDVPYFLVNKPAVYNLNKQEVTPIKFK
                     HNPQVLLIIHPDIYEKENVVKDFLDYGESLQKPLEKVVELAESGSLRELGSVLFNDFS
                     ETIIKHTPALRTVLNDLKQLNVEAYGIAGTGTTIFALSDNRHLLRFISDKYRKQNYQT
                     VMTKVIR"
     gene            complement(3647..4075)
                     /locus_tag="PLU08_00885"
     CDS             complement(3647..4075)
                     /locus_tag="PLU08_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70299.1"
                     /translation="MKNKKKLFWLILPFTLIMFALLFALYPTPWGAKEPYKVFHSPEK
                     HLKEVEKRVNQKIEEREMDYTNLRALPLYDEYNKFSFVLVEFEPQDFMILKVYRTSII
                     SVKRTIYLRDTNNSFGVHTENNNENSQFNPYQNCDTSNNK"
     gene            complement(4378..5163)
                     /gene="rsmA"
                     /locus_tag="PLU08_00890"
     CDS             complement(4378..5163)
                     /gene="rsmA"
                     /locus_tag="PLU08_00890"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOQ70300.1"
                     /translation="MNKEEIITLLAKENLSPQKRKGQNFLVDENVARKIVSFLEPNYI
                     DVIEVGPGLGALTKELLLKTVNFTAIEIDKGFVRVLRAKYPHLNIIEKDFLEYSVPRE
                     TKAVISNIPYYITTKIIEKVLLEAENLKKFVFMTQIDVKERLLAKSGTKAYSPLRVLL
                     SLLGDVEQKLVVTADKFYPVPHVDSAVFCFTRYKTDIPVREFYSFLNNAFLNRRKTLY
                     NNLKNIYDKEDILTALKKHRINENVRAEALSPNDLLTLFETLR"
     gene            complement(5156..5695)
                     /locus_tag="PLU08_00895"
     CDS             complement(5156..5695)
                     /locus_tag="PLU08_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4093 domain-containing protein"
                     /protein_id="HOQ70301.1"
                     /translation="MSSKTLLNCIFVAEGKRDEERLKKLDIPYIVRTDGLNVPRETTK
                     HLQELAKIHEVIILTDPDGPGQKIADKLLQEIPNAKVIKVIKKKAVKKGKVGIEHIPL
                     PLLAELIKPYKQETFTTSSDVTLIKLLDLGLSGPNSQFKKIKLATKYSLLVASLKNML
                     TQIQLLNISYKELKEALSE"
     gene            complement(5692..6459)
                     /locus_tag="PLU08_00900"
     CDS             complement(5692..6459)
                     /locus_tag="PLU08_00900"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOQ70302.1"
                     /translation="MFFDTHCHLNDKRYENDIEGYVERAKAVGVNYLLIVAWDVKSSK
                     KAIKIAERFNNVYAAAGIHPVDAVKTPKSDLKELEKLLTHPKVIAVGEIGLDYHWIKD
                     EKERAIEHEFFIAQIELANKYKKPVLIHMRDATSDTYEVLKSNIPRYQGIMHSYSGSK
                     EMALRFIDLGMYISLAGPVTFLNAREPKEVAKVVSEERLLLETDAPYLTPHPYRGKLN
                     ESANIPLIAKTIATIRNVSVEKVAEFTTKNAKTLFKL"
     gene            complement(6462..7784)
                     /gene="mnmE"
                     /locus_tag="PLU08_00905"
     CDS             complement(6462..7784)
                     /gene="mnmE"
                     /locus_tag="PLU08_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOQ70303.1"
                     /translation="MLDTIVALATPPMMGALAIIRLSGPEAFNIVTKSFTKDLTKLEK
                     STMVYGKLFDDEELVDEVIAFCFKGPNSFTGEDVVEISCHGSMLIANEIIELMIKNGA
                     RLAGRGEFTNRAYLNGKLDLVQAEAVNDMITATTKEAKKLSLLSLVGETSKIVYPIRE
                     RIADVMSLIEVNIDYPEYRDIEEASYALVISEVDYINAEIKKLIDQGRQGQIIKEGIK
                     VAIVGAPNVGKSSLLNALLKEDKAIVTDIAGTTRDVVEGEINLHGLRLLLLDTAGIRE
                     STDAIESIGIEKTKKSIADADLVIYVKDAENVSKKDEFTGLIKGKKVITVYNKGDLVS
                     KKDPNRLYISALTNDIGALEKAIAKVVGVDEDSYLRPSLNNARQLALLRKAYGELEIA
                     KQEALDEVAIDLVSVSLQQAFFTIKEILGENVDNDLSKEIFSRFCVGK"
     gene            complement(7853..8494)
                     /locus_tag="PLU08_00910"
     CDS             complement(7853..8494)
                     /locus_tag="PLU08_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOQ70304.1"
                     /translation="MRIFEAKNLDEVLALASEDIGIQTKDLVYEVLEEKKGLFKSSIK
                     ISVYETTDYIEYAENYLRLVVESLGFTPEFTTSLDGEVIKIEMSTNHNSILIGKGGKT
                     LLALNEIVQVAMTVKFKKRIRVLLDINNYKNDRYRRVTGLARKVAGDVARSKQDATLD
                     PMPSDERRAIHNALSEYRNVTTESIGAGKERQVIIRYKKPEKKEKAPKEPQDE"
     gene            complement(8494..9873)
                     /locus_tag="PLU08_00915"
     CDS             complement(8494..9873)
                     /locus_tag="PLU08_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOQ70305.1"
                     /translation="MKKNKKRIIKLGAIVAGLFVLSSCTASFCSAKDVGYMLYAQDSG
                     LVTEVVEGTETKVHNKVLHKIILDAQSQGYATPSLEYYEKLDQKVLDYAILNFDNSEN
                     LEGAALNAAALKQNGYLKFLGTKGATVVTADGNELWVNWTIWNKEIGAEIGYENVPDR
                     DFANFYKTQIYNKIKANRACIALYDGEYGPEDSKIPVEAKTWKGAWKKGFIEGLIVYP
                     VAALLEFLTKSFGAGGWGQIWAILVTTIIVRGLLVLMTLKQTVGAQKMQALQPELAKI
                     QQKYPNSDTNQYERQALAQAQMALYKKHGINPLGSLLVMFIQFPIFIGVWGAMTGSAS
                     LASDSVLGLNLSAQLGQSMINGWFKGGWWTAWVLFILMTAAQFVSTKLSTWLNKSKTK
                     EIGKTTANPAADKQQRQSKMMMNIMFIMIVFMSFTLPAAMGVYWFVGAIISIVQTVVV
                     HNVMKGRKQ"
     gene            complement(9875..10198)
                     /gene="rnpA"
                     /locus_tag="PLU08_00920"
     CDS             complement(9875..10198)
                     /gene="rnpA"
                     /locus_tag="PLU08_00920"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOQ70306.1"
                     /translation="MKREHRILKNEQFQNIIKNGKLTKTPLCYGYFASNELETLRVGV
                     AVSKKIGNAVTRNKIKRQIRAVVRPYVKTHTGDFIIVAKKEINDSSFGDLVDAIAKIF
                     DSNGD"
     gene            complement(10222..10356)
                     /gene="rpmH"
                     /locus_tag="PLU08_00925"
     CDS             complement(10222..10356)
                     /gene="rpmH"
                     /locus_tag="PLU08_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOQ70307.1"
                     /translation="MKRTYQPSKLRRARLHGFRARMATPGGRRVLRNRRAKGRESLTV
                     "
     gene            10863..12218
                     /gene="dnaA"
                     /locus_tag="PLU08_00930"
     CDS             10863..12218
                     /gene="dnaA"
                     /locus_tag="PLU08_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOQ70308.1"
                     /translation="MNMSISEITQIWDRTLIKLQEKIQDRRVYGAFFPDTYIHSINNG
                     IITVIANSSLAKSMLNTNYRDVIEETVGEITQSNYQISFIEKSEIPRVSEIISEKKET
                     FFKNAKVNSNLTFDNFVVGSSSNLEAYQAAVVVANSPGKLYNPLFLYSASGLGKTHLL
                     HAIGNHVKQNSPNLKVLYITADEFTDEFIRFVRDNPGGSLKDFFRTVDVLLMDDVQFL
                     ADKKSTSETFFHLFNLLVGSGKQIVLTSDRHPSELKGIEERLVTRFNSGLQISISAPD
                     LETRKDILRMKISLNNLDIEDFDEEVITFFAENFTNNVRELEGALNRLLFYVVSIAPT
                     KHVTMEVALNSVQSLINVKDAVTKLSETKIINVVADYYNLTPALIIGRNRTSQVALAR
                     HIGMYLTREILDLPFARIGKAFGGKDHSTVINAITKVEKQLKEDPALIQAINEIKERL
                     Q"
     gene            12347..13480
                     /gene="dnaN"
                     /locus_tag="PLU08_00935"
     CDS             12347..13480
                     /gene="dnaN"
                     /locus_tag="PLU08_00935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOQ70309.1"
                     /translation="MKFKINREQFLKGLSIASRPTQAKSPIPILGNILLSLDETGLTL
                     LGSSNELAIRTHIPMRVNDLEVIRMFLPGETLISSKTLNESIRKMEGNEIDVEIIDRT
                     IAQISDGRTEYRLGAIDSKDYPDIDLSQDGTVFEIGAKDFIKLVDQTSFAASVREQRP
                     ILTALNLNAVNGTLTAVATDSARLANKVVTLKDNVSFVANVPARIMNEVAKLLETMVG
                     IETIEVSASANKINFMFDDTLVVSRLVAGEYPPTRSIIPKTHASFLEANANELLKAMD
                     RVSILYAAEKDNIVKLSLSPESVVVSARSTFSAESAREEVALCKYVGEPLEISFNYTL
                     VSSAIKATGAEDVTIGFNGGHKPFSIRVATDDSLVLIVTPVRT"
     gene            13649..13852
                     /locus_tag="PLU08_00940"
     CDS             13649..13852
                     /locus_tag="PLU08_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOQ70310.1"
                     /translation="METKPYITLQQALKKYNLVSTGGEAKFVIQNGEVKVDGILETRR
                     GRKLYGGEIITYAAQKVVVKDVD"
     gene            13842..14909
                     /gene="recF"
                     /locus_tag="PLU08_00945"
     CDS             13842..14909
                     /gene="recF"
                     /locus_tag="PLU08_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOQ70311.1"
                     /translation="MLISDLELLNYRNYPKLNVIFTSGLNVIVGDNGVGKTNVVEAIH
                     LFAFAKSFRTNDTKALIKHDTAKAIVSGTIHIPSRTDISIELTNRSKRILINGKVLPK
                     VSQLSKYVRTTTFEPEDVLFFDASPAKRRKFLDANLSSADEVYLATLVRYEKLLKERN
                     VLLKTDDNDEALDLIDKLFIELAKTIVEKRTRFIRELNKVVNEVLLKLSDEHLSVRLT
                     YESSLGSVQNIERNGLKRLAEVRAKENILESTTLGPHRDDLIATINGHGVKEHASQGQ
                     KRLIVIALALAPYFLEKDLHKKPIIILDDVLSELDKRHQERLIELLLTCTQAFITATH
                     YDNHAHAIYEVTSDRELRRIT"
     gene            14969..16909
                     /gene="gyrB"
                     /locus_tag="PLU08_00950"
     CDS             14969..16909
                     /gene="gyrB"
                     /locus_tag="PLU08_00950"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOQ70312.1"
                     /translation="MRARESYTAEDIQVLEGLQAVRQRPGMYIGTTGLPGLHHLVWEI
                     VDNAVDEALAGFCTTIDVSINKDGSVTVADNGRGMPVGIVSQTGKTAVETIFTVLHAG
                     GKFGKGAYQVAGGLHGVGAAVVNALSERVEVNVYKDGDEYYLAFNNDGHTEGELKKVG
                     PTNKRGTRVTFKPDPLIFVESVEFDYKTISTRLRQMAYLNRGIRINVEDRRGEELKRE
                     EFYYEGGIAEYVQHINKNRTPITQDVIFVEGKGDVEINGQKSHILVEVAIQYTTEYKE
                     TIFSFANNIQTGDGGTHEQGFRLAVGRVFNNYAKEAKIHKNEDDRFTYEDVVEGMTAI
                     ISVKHPNPQFEGQTKGKLGNTDTRAVVSQVLGEKLEEYLLENPNEAKLILEKISLSSK
                     ARRAATLAREGVRRKDGIEFTTLPGKLADCSSRNPEESELFIVEGNSAGGSAKNGRNR
                     HTQAILPLRGKILNVQKAQETRIYENAEIGNMIQAIGAGIAEEFDITKARYHKIIIMT
                     DADVDGSHIRVLLLTFFNRFMRPLIQNGYVYIAQPPLYKVDYRGKQYYCYNDEQLEKL
                     KVDLDLRPGYPFQRYKGLGEMDAHQLWETTMDPANRKLLRVHIEDAIEADQVFEDLMG
                     EKVEPRKEFINENALFVKNLDV"
     gene            16920..19448
                     /gene="gyrA"
                     /locus_tag="PLU08_00955"
     CDS             16920..19448
                     /gene="gyrA"
                     /locus_tag="PLU08_00955"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019243409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOQ70313.1"
                     /translation="MVEDNIKDMLEEEEKHELEGIDTGIHPVDVVDEVRKSFLDYAMS
                     VIVSRAIPDVRDGFKPVHRRIVYGMYESGMTPDKPFKKSARIVGDVMGKYHPHGDQAI
                     YSTLVRMAQPFSLRYPLVDGHGNFGSIDGDEAAAMRYTEARMGKLATEMVRDINLNTV
                     DFGGNYDGSEKEPEVLPSRFPNLIVNGSDGIAVGMATYMVPHNLSETIDAINLVAKNP
                     DVTPREIFENGMLGPDFPTGASILGRKGILDYFETGEGSIIVRAKTHFETKGDRTWIV
                     IDEMPYKLNKATTIEAIALLVRNKEIDGITDIRDESNKEGIRVVIELRRDVVPEVVLN
                     NLLKQTRLQTSYKITNLCLVNKAPRILNMKELIVEYLNFQVEIIRRRTEHQLSQAEAR
                     DHIVEGLLKATDNIDEIVSIIKNSESGEEASQKLMERFLFSEIQTREVLGMTLRRLTG
                     LEIDKLNKEREQLLANIERYRLILSARDHMVDVVLEELEEIKAKYGDERRTEISDDLA
                     DIDNEDLIPVEDIVVMLTNQGYIKRVDPEEFRTIKRGGVGVKGMRTKEEEIIDIILHT
                     NTHTDVLFFTSKGKVYRKRGYQIPQYRREGKGLPVINLLNIEKEEAVRAIVSTDTYGE
                     DHYLLYVTRNGVTKRTTLAEFSRINVSGKIAIDLREGDELMDVKITDGTAIIGIGSSN
                     GKMASFYETDVRAMGRTAAGVRGIKLDEGAYVVGVTTSLEGALIFALTTKGYGKLTPA
                     NDYRLTKRGSKGVITIKESERTGTLAGIRAIKGDEDLFVTTASGMVVRTNFKEISTTS
                     RNTMGVRVIRLRPDDVVCSFAVVEKGIAFDEDDHEGEGTPSHEE"
     gene            19451..20452
                     /locus_tag="PLU08_00960"
     CDS             19451..20452
                     /locus_tag="PLU08_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70314.1"
                     /translation="MKILISFPHKKIDVFFEGAMLEYQIKKACQRDDVTLVTKFSPDI
                     DIANFINLNSQSSKVIRQSIAASIPTLLWMFFANNDTYARVIETRKDGELYIPLSRLE
                     LINMMDGIVVPAQEARVILRKLGVKIPIFVIYGAVDVRRIDELKTSKTDVFRRYFRIN
                     DDQKYAITVMNIKASEHIDQLNDLAAAVPNYNFYVFISGGTSFIDRLRLKAMDKLTSP
                     NLIVTKLVPEDVYRMGVIGASYFIDLGYDKMNVMTLFEPMYLKVPLILRKNVIFKEIV
                     DKTKAYIVNDYNGAAYLIRTEAETDKITEKAYEYTQNYNAALFTEAIYNLFYKIYTR"
     gene            20708..21985
                     /gene="serS"
                     /locus_tag="PLU08_00965"
     CDS             20708..21985
                     /gene="serS"
                     /locus_tag="PLU08_00965"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOQ70315.1"
                     /translation="MLDINLIRNEAEKVKSLLFKKGWDFDPEPILSRDEKRRKLRVEV
                     EEAKAKLNKLSASVPVVKKAGGDIQAIFAEVKDLKAENEAKEKELKKLDEEVFNLLAE
                     LPNLPDEDLLAGGKENNKVVYTFGKMPKFTFPIKDHVELCESLGLIDYKRAAKISGSG
                     TWIYTKAGALLEWGLLNFFVSEHLKAGYDMILPPHLLNYESGFTAGQFPKFKEDVFWL
                     EGTEPRKFLLPTSETALVNFYRDEILKEDELPKKYFAYTPCYRSEAGSYRTEERGMIR
                     GYQFNKVEIFQYTKEQDSSKAFEEMVAHAAGLVEKLGLHFQISKLAAGDCSHSMARTY
                     DIEVYIPSMGIYKEVSSVSNARDYQARRGMARYRDKETNAVKYLHTLNGSGLATSRLI
                     PAIVEQFQQADGTVLVPEVLQAFVGTKVLHPDW"
     gene            21995..22091
                     /gene="ffs"
                     /locus_tag="PLU08_00970"
     ncRNA           21995..22091
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLU08_00970"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            22096..22551
                     /gene="tadA"
                     /locus_tag="PLU08_00975"
     CDS             22096..22551
                     /gene="tadA"
                     /locus_tag="PLU08_00975"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOQ70316.1"
                     /translation="MNKDEKYMKLALKEAHKAAAIDEVPVGAVIVLNDKVIARGFNKR
                     EINNDPLGHAEMIAIRKASKKLNNWRLVDCELYVTVEPCAMCAGAIMWSRLKRVVYGT
                     GDPKGGAMGEAFNLFDERIVNHIPEIKGGVLAKECKEVIQSFFRNKRKI"
     gene            22650..23468
                     /locus_tag="PLU08_00980"
     CDS             22650..23468
                     /locus_tag="PLU08_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70317.1"
                     /translation="MQKRILIVISLLISVVLNGCTKPQKEQSELEKFLIKYKEVYPDG
                     YNFQTGFYSITSFTKSKVDSSDYFEIETYNIKAQLQSIPYGVLWNFEANMMQLNRSLV
                     LGAGREGPIENFENILWDGEYLHKTIKCNNYIHKETWTEKTYGTDQVETLLINTKLYT
                     IEYVIRILKTKLSYYEKYVFDKSNVDFINNSLIVSTFFSEFEIYKIIQIDFDENFVIS
                     RYQFSYIDLGRVPDEFRPRQITQTLKPIDAIEVNAQLEYEEELIVEKDIFTIQI"
     gene            23968..24789
                     /locus_tag="PLU08_00985"
     CDS             23968..24789
                     /locus_tag="PLU08_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70318.1"
                     /translation="MRKRILVIISLLFSVVLNGCANKEKEKSELEKFLIKYKEVYPDG
                     YNFQTGFYSITSFKKSKVENTDYFHIETYNIKAQLQSKPYGTLWNFEANMMQLNHSLV
                     GGVGTEIPNENFENILWDGEYLHKTIKYNNGYVPKETWTEKTYGTNQIETLQINTEID
                     KIDYIISTLETKLTDLEKHVVNKSNVNFKNNSLIVSTFFSKLETYKIIRIDFDENFVI
                     SRYQISYTDLGRVPDELRLRQITQILKPIDAIEVNAQLEYEEELIVEKDIFTIQI"
     gene            24930..27482
                     /locus_tag="PLU08_00990"
     CDS             24930..27482
                     /locus_tag="PLU08_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ70319.1"
                     /translation="MLVLKDIYKDYYVAKKPLHVLKGLNLTFPKQQFVTILGPSGCGK
                     TTLLNLIGGLDKYTSGDLIIEGKSTKEFKDRDWDAYRNHRVGFVFQSYNLIPHLSVIE
                     NVEISLTLSGMGSSERREKAKQALLSVDLESELYKRPNQLSGGQLQRVAIARALVNDP
                     SIILADEPTGALDSETSEQVMKILKEISKTRLVVMVTHNEDIAKRYSDRVIRMLDGRI
                     TSDTDEAQSKKKRKVKEPTETEKNKGTSMSFLTALKNSYKNILTKKGRTVFTALAGSL
                     GIIGIGLVAAVSNGFQNYINRLERETMANYPIAVFAQTMDMQAGIKKPDKNTEYPDGE
                     EIVVYNENDTETTRITITNNRITSEFLDLVDGLKEDGLAASTMVNYPTQMQILAKPPT
                     DNARTVQWPYYVSVTPLPFGVFTQLPGDEAYITEFYDVIGKDSKFATERNEIMFVLDK
                     YNRISVQALERMGIVDNSANVQKLNFDDIIGREFKMVHNDDIYKKALGNIDDYVVLDF
                     MGQPRTIEKYRFQNPQYLYDDPTVGEMLKITGILRPKPGIQFEMLAPGMVYTQALNKY
                     MHETNKHNKVAKAQVNNVVIREANITAENINTAIEFYSATTDWSGVKPVNPTTIRIDK
                     FLSNLKIYGVDLADISKDNAIYETLLKELLNTAYSPVESVAIFAKDGDSKKEIKARIN
                     EYNNTREDEKDKIVIFDAVEFVTDSIATMIDVVSIVLIVFTSISLVVSGFLIGIITYV
                     SVIERTKEIGILRAIGARKKDISRLFNAETFIIGLLAGVIGVGTTYILSIPINVILNN
                     LFPEQNIGQIAFLVPLAALALIVFNILLTVTAGLIPSKMAANKDPVVALRTE"
     gene            27502..29988
                     /locus_tag="PLU08_00995"
     CDS             27502..29988
                     /locus_tag="PLU08_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOQ70320.1"
                     /translation="MEEFVETKKKRPGTATTIIKKSEIKVDEVIRYTPKHDEGLSTQQ
                     VNARKVAKMTNEKPNKGVKSIFSILMRNFFSFFNVLLYAIGAALIYIKEWQSLLFLVI
                     LFSNILIGLIQDFRARRTLKKLLILNEQKVTVVRDGIELLIPSEDVVLDDIVKLKLGD
                     QIAADGVVVHGSVGVNESLLTGEPNIIHKAKDDRVLSGSFVTTGTCYYQVDTIGKLGY
                     AQKLQLRAKVFKRPTSELLNSINSLFKVIGFIVIAMALLLVFNAFYQGSTFEDSVRSI
                     AGSLVAMIPSGMYLLTSMTLAVGVIRLGTKNTLVNEMYSIEMLARVNVLCLDKTGTIT
                     DGTMKVNEVVPLKGAKDIDLNIIVASILRATKDENATAIALKNHFKTRKYMEYTKVLP
                     FDSELKCSGAIMEGKTYIIGASSMVLGPKAQKQVEEVERIYLERGLRVLVLASSRKPF
                     IAKKLPIDMTPLALIVIQDNIRAEASEVIKLFQDAGVEVKIISGDDPVSVSEIARQVG
                     INNAENLLDFSKIDDPSHHSLEDFTIYGRVRPEQKEGIVTKLQSDGKVVAMVGDGVND
                     VLALKAAQCSIAMGAGSPAARGVSHLVLLDNNFDVLPSIVDEGRRAINNLQKTWSLFL
                     VKTIFAIIFSFMFVTAGLLGPKGSGIRYPFEPKHLYLWELLSLGIPAFFLSMQPNKER
                     VRGTFMDNVISRSVPAGLLMVAGAFLLYISKNFGEMFGAEISDTSMEMITMSILTITF
                     LSFVILYRNSLPFNRYRLTLFLVLSLLAIGVIILDNTVGLTFTEKYFLEINISSLEPI
                     HYAILTPINLILTIMYILVDNKIVKKLRRE"
     gene            29992..30909
                     /locus_tag="PLU08_01000"
     CDS             29992..30909
                     /locus_tag="PLU08_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOQ70321.1"
                     /translation="MKIRISQEVAYALANNLPVVALESTIISHGMPYPQNVEIALKVE
                     EIVRANGALPATIAIIGGDVVVGLDEDEIEELGQRKDVIKVSRRDLAYVMATKKWGAT
                     TVAATMVIAAKVGIKVFVTGGVGGVHRGATETLDISSDLDELARTNIAVVCAGPKAIL
                     DIEKTLEYLETKGVPVLGYKTDKMPVFYTTSPSLNVLYRVDSSSEIASIVNASHALGL
                     ESGTLVVNPIPKEEALPYKDIEKVIDEALEEMKKAGVKGKEETPYLLSKLNELTKGKS
                     QAANEALVINNAKLGASIAKHICRELYED"
     gene            30899..31444
                     /locus_tag="PLU08_01005"
     CDS             30899..31444
                     /locus_tag="PLU08_01005"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HOQ70322.1"
                     /translation="MKTKARLGNSLKKFMATMPLDTISVINITKDCKVNRQTFYYHFR
                     NIYDLLTWVFLNEEIEKPSNNELEEAIFIVLRYVKHNEAMIENTLASAAKDLVREFLF
                     NYLYNVCLNYIDKKDPFEKLTLNERKLLARVYGAGIASSIISWIEGEYLFPEHEFVMN
                     LVTFFNDFPLDAINYKGHGYA"
     gene            31437..32609
                     /locus_tag="PLU08_01010"
     CDS             31437..32609
                     /locus_tag="PLU08_01010"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOQ70323.1"
                     /translation="MHSFNLKIQTELIFGNGRHTEVGSIIKRYGKKNVIILYGQNHAV
                     RSGLLEAVTNALDAENIDYVLYGGITPNPEVFYVREAKAIAKEYAVDFILAIGGGSVI
                     DVAKSLSVNFYYEGDALDFSKRIVTPKKALPLGVILTHASAGSEMSASCVISDSTNNF
                     KQGFRNDLVRPLFAITNPELTYSVSMYQTAVGIVDTFMHSLERYFNKSDKIELADRFA
                     EAIFVTLRECAETLIADPLDETARANLMLASTFSHNDVTSMGKKANLVVHTLEHALSA
                     LYPSIAHGHGLAVLYPAWLFVYFEELKEKLDRFARVVFNLHHEDIDTNAKEGILALKE
                     LFMRLGLTTTLSSLGVKREDIPTLADIVTENGTKELYHDQKNLTRDDIIKLYESCF"
     gene            32628..33749
                     /locus_tag="PLU08_01015"
     CDS             32628..33749
                     /locus_tag="PLU08_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70324.1"
                     /translation="MKKEDLLSILVYLVMLIIALFIGLRIIQPALDALDLVTDLQRYG
                     FAILTILVGIIINVILFELGHVFGALLGGYSVISVNILGLAYYKTKSGWKFSFRRYEG
                     LTGETKIIAKSDKTKPRLYLFGPTIMVLLEFVVAIVIFLLTKYNQPIHHQVLIVAGIG
                     AMLLVYNIMPFKLDNFTDGYYIVLLGKKVNVEAYNELIRIESLVYNNEKVTDIKEFDE
                     VTTMTARLSLYRLYQLIDEKAWDKALSLIDDLEKNASKVEHEFIARIRAQKLYIYLLT
                     KASEAASAYWFDELSAADRKFISNDLTIETMRVYLLYSGLVTKSQSECAFVLSRAPKA
                     LRARINDFRKEEEIALFNEAYEKIVALPGNENLKLPVLK"
     gene            complement(33807..34316)
                     /gene="mscL"
                     /locus_tag="PLU08_01020"
     CDS             complement(33807..34316)
                     /gene="mscL"
                     /locus_tag="PLU08_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOQ70325.1"
                     /translation="MRKFFKEFKAFIARGNVLNLAVGIIIGGAFNAIITSLVNHVIMP
                     PISLLLGNEVSLLKWVIRPEVRDAADNVVTAEIAISWGLFVQAIINFLLIALVLFVIV
                     RVVTRLTEKAEALRAKLLHEEAAAAPEPEPEPEPEPSEEVLLLREIRDSLVAKKQEAK
                     ATKKKEPKK"
     gene            34492..35346
                     /locus_tag="PLU08_01025"
     CDS             34492..35346
                     /locus_tag="PLU08_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOQ70326.1"
                     /translation="MPKFKIVVDSTIDLSPEQYKKLGAIVLPLNVHIGEETFQDGVDI
                     NADKLYEKVAETKTAPKTSAVAPGVFKEVFEKLKAEGYTGALFVGIGESLSATAQSVR
                     LAAEEVKDFKVRVVDSHSLSTGSGLLALRAYDLLNEGKTLDETADYLEKIAPKSRAQF
                     IVDNLEMLAAGGRVTGIKLFFGRILRAHPFLQVNNDKLEVVATPKGKSERALDLMLQV
                     AREEISAGLESPRVFITHSHGGERIDYLYDRLKEFVDPKNILISEAGAVISSHCGAGT
                     IGILYIRK"
     gene            complement(35391..35768)
                     /locus_tag="PLU08_01030"
     CDS             complement(35391..35768)
                     /locus_tag="PLU08_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOQ70327.1"
                     /translation="MRLTPARKLVLNLFKTKSSLLCAEDIERLLKNESIFLSTIYRAL
                     EYFEKENVLGKSTINKTAYYYLIDGKHHHYMICTTCLARFPIDCKLDSMINKNMKKHN
                     FVPTSHDLTIYGYCENCAKLQNN"
     gene            complement(35746..36585)
                     /locus_tag="PLU08_01035"
     CDS             complement(35746..36585)
                     /locus_tag="PLU08_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOQ70328.1"
                     /translation="MFSFLQHTFMVYALLLALLIALAAALLSPFLVLSKQSLIADGMA
                     HIAFTGVVIGLLFSNEPLYIALPFALLASIAITFLTERANLDNDAAIGVVSVFALALG
                     LIIISKSDGFNRSVESLLVGSLLTVTVPEIIGASVLALLVICFILIFYRKLIITTFDV
                     THARFAGVNTMFLKYALSALTSIFVVVGVRAIGALLISAFILFPTLISRQLSKSFNMT
                     LWGGVIISLIATFIGITVSYHFTLPTGPAIIMVFTTFLIVAYIIRIIIAKRRLKDAPN
                     ARS"
     gene            complement(36578..37285)
                     /locus_tag="PLU08_01040"
     CDS             complement(36578..37285)
                     /locus_tag="PLU08_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOQ70329.1"
                     /translation="MPTSSRKMSQTSNKPLAVKLTDVSVNFGDVIALKNINFSINKGM
                     LLYVIGPNGSGKTTFIRLLAKLLEPTHGTIEHSDDRLGYLPQKLNINPNFPITVEEVI
                     YSGLAKQSLLISKGDRELIKSWLKKMQIPHLLKKPMAHLSGGQQQRVYIIRALISNPD
                     FIILDEPTSALDPSFRSFFNDIITALHHEGKTIIFVTHELHGALCDCASVLYIDQEIR
                     FFGGLQEYFEKEKNIHV"
     gene            complement(37230..38144)
                     /locus_tag="PLU08_01045"
     CDS             complement(37230..38144)
                     /locus_tag="PLU08_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOQ70330.1"
                     /translation="MKKKLLLLLPFLLLAGCKAREKPDIVVTSFVSYDAVTNIVGDKL
                     KVKNIVPWGSELHDFEPTPRDIEDINNAKLFVYTHPALETWVKDLVTNKNSFDMSIHS
                     DVEYACKVKKQEHHDHEHDHDHTLHYWTDPHIYIRVMNDLLTVLIDISPANETYFTEN
                     HNNYTARLLATSNDFRDFLFPPLEKPPLFFAGHNALGYFAAAYKLTIKTISESYKPDV
                     DFLSQEIIDFMYEIKTFDSHYLFIEELVEPRVANLIKNELAKENYAITLLELHGYHNI
                     TAAQAKEGITYADIFAQNVANIKQAFGG"
     gene            complement(38267..39586)
                     /locus_tag="PLU08_01050"
     CDS             complement(38267..39586)
                     /locus_tag="PLU08_01050"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOQ70331.1"
                     /translation="MNVKKFFELAKEKGLEAAELVIQKNKGIEMSVYDESVEKYTLAS
                     TINVLARGIYKGKMGFAMSEKDDKTTAQYLVAQIKMSAGVSETEDLSIIFEGSPKYRK
                     KSFFNKELATISESTKLESLFTIEKALKKAHPLITSVSTVQYSEIESDYKLFNSYGLK
                     LTNKQNYFYYGTWVVAKEGDDVKSHVEVFLDNDYSKFDADTFVKKAADGVIEKLGGKP
                     CATKDYPVVFNPKMTASLLAAYLTSASADRVEKNTSLFADKLNERVASSKVTVLDAPL
                     TANLFYRYFDDEGVAKTNKPIIKNGILQTFFHNLSTATKMGVEPTGHGRNTGGRISVG
                     ISNITLKPGRNDFDTLIKDIKEGVFITELMGLHSGLNTTSGDFSLQAAGFMIRDGKVA
                     EPVNLITVAGNLVKLFNDVKAVANENELQLSSYTTSSILVKSLKIAG"
     gene            complement(39583..40977)
                     /locus_tag="PLU08_01055"
     CDS             complement(39583..40977)
                     /locus_tag="PLU08_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOQ70332.1"
                     /translation="MITKKLALEVLNVALSTGGDFAEIFLEETYSGNYTVENGKTQAP
                     ATSLTYGAGIRILKGLQSVYGHTSDLSKKGLLNLATALSAGFDGQRVLSVEKLKNVRV
                     RKNHELEKSLELVDINEIVEKLKITNKVMKDYDEKIVRAISVLLTDVRNITIFNSEGN
                     AFTDKQERVRLVFQAIAMAPGAFEYSYNGPGAHKGFEYVEGLDLEKIARDVADVAVRK
                     LTAAECPSGKMPVVIGNGFGGVIFHEACGHPLEASSVSKGLSPFAGKVGKQIAHPAVT
                     AVDDGTIVNGWGSNNIDDEGVKTKKNVLIKNGILQSYLVDRFNGRRMGIESNGTGRRQ
                     NYTFEPTSRMSNTYIAAGKYTPEEIISSVKLGLYAKSLGGGQVNPATGDFNFACDEAY
                     IIRDGKVAELVKGASLVGNGADILMKIDMVANDLERAQGMCGADSGSVPVDVGQPTIR
                     VSEITVGGRGGALQ"
     gene            41793..41996
                     /gene="rpmE"
                     /locus_tag="PLU08_01060"
     CDS             41793..41996
                     /gene="rpmE"
                     /locus_tag="PLU08_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOQ70333.1"
                     /translation="MKQGIHPKTQKITVTCVSCGTEFEALSTKKEIRVDTCSNCHPFY
                     TGKQRFTQADGRVDKFNKKYGLK"
     gene            42057..43637
                     /gene="metG"
                     /locus_tag="PLU08_01065"
     CDS             42057..43637
                     /gene="metG"
                     /locus_tag="PLU08_01065"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOQ70334.1"
                     /translation="MKKRFYITTPIYYPSAKLHIGHAYCTTLSDILARYNRMIGKEVY
                     FLTGSDEHGQKIAENAAKANKTPQKFVDDIVKTFKDLWEKLAITNDDFIRTTDPKHIA
                     TVQKIFTKLLEQGDIYLGSYEGWYCTPCESFWTTTQVGEEKLCPDCNRSVHLASEESY
                     FFKTDKYVEGLLKKYEETPNFITPESRKNEMVNTFIKPGLSELAVSRTSIEWGVPVRE
                     NERHTMYVWVDALINYISALGYLSEDDELFHKFWQSEDVEIVQILGADITRFHTIYWP
                     MILKALGLRQADRYFVHGLLMMKDGKMSKSKGNVIDPLPLIDKYGVDAVRYYLAREVV
                     FGNDGQFTPEQFVERFNVDLVNDFGNLLNRTVSMIEKYCDGVIPLYRGQVTEFDTEIE
                     DLAINVVKEYHEHFSDLKVTEAITTAFKIISASNKYIDNTKPWELNTQGKKEELDSVM
                     NHLANNLFIGAILLQPVLVNAPKELFKQLGVPQDKQDINLLNKYGVLGGEKVNKGEPL
                     FPRLKVNEEVEYIKNLMK"
     gene            43637..44989
                     /gene="rlmD"
                     /locus_tag="PLU08_01070"
     CDS             43637..44989
                     /gene="rlmD"
                     /locus_tag="PLU08_01070"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOQ70335.1"
                     /translation="MIKENDIYVVTGGDYTSEGIGLAKVDNFPIFINNLIIGEEAEIK
                     ITKVMRNFATGKIIRFTKMSSDRITPVVPETIHLAGCQFTQLDYAKEVEYKLSKVERA
                     LRVIGGIDFKIEKIHTAKNPYFYRNKTVLPFGKRKSGKVYTGLFRHNTHEIIAMEKTH
                     LDDELASIVIAEVRKLIEKYNYEVYDEKSHTGQVRKVMVRVSHYLREVMVVLITKDEQ
                     IPNLDAFCRDLVAICPNITTIIQNINSEDTNVILGKKEKVLYGPGFINEELHGLKFTL
                     SSKSFFQVNTLQAEVLYERALTLAGITKNDVVLDAYSGTGTIAILAAKKAKQVIGVEI
                     VKDAVKDAEVNAKQNNITNVKFFKDDAGAFLTREIKNYNFSVVIVDPPRKGLSDEFVN
                     TLLRALPPKIMYVSCDPATLARDLKKLTKKYNVEHIECVDMFPRTAHAETIVSLILRK
                     "
     gene            45449..>46520
                     /locus_tag="PLU08_01075"
     CDS             45449..>46520
                     /locus_tag="PLU08_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOQ70336.1"
                     /translation="MKKNKKISKKTRENWRYLVTHFTPEYIEAMTDKKEYVVGWGSDN
                     KSFCYLVEVGTKELGEIRGANSSKFGLWYGTHGEDKEIKYRATKQHFNCSVNKAFEDI
                     KIALAKLIRETRELTEFKDLESLFSGMFKYKIMYLYNPSIMIPCFLLEDLQYFEEYLI
                     GKSSKTFENAQKALIKYKDKHFSKKDNHDFMIFLYSIREKYNVIQTKELNEEADNKLN
                     KKLSKEKESTYIYITHPVDKVSLKKGTGGGLYYPRDVKMAAIALKNANYKCEANSNHK
                     CFLRKSNGMPYTEVHHLIPLAYHYKFDKSIDIPENIVSLCSNCHNEIHYGRDANKMIK
                     KLFNKRKKKLHEAGVDVSLKELF"
BASE COUNT        15526 a         7928 c         8642 g        14424 t
ORIGIN      
        1 tcactgttcg tttacttgac ccaccgttac acgaattctt accaaccgaa gaagcacaaa
       61 tcgttgcttt agccaaagaa cttaaagttg atgtcactgt tcttcgcgaa cggattgaat
      121 cgctttcaga atttaaccca atgatgggtc accgtggtgt gcgtttagct gtttcttacc
      181 cagaaatcgc tgtgatgcaa acaactgcta ttattagtgc cgccttacgt gcacaaaaag
      241 acactggtgc cgctatccac ccagaaatca tgattccact agtgctagac gttaaagaat
      301 ataaatacgt tgaagaatat gtacgtaaaa ccgctgatgc attaattaag aagagtgggc
      361 aaaaacttca ttacctcgtt ggtacaatga ttgaaatccc acgagcaacc ttacttgccg
      421 accaaattgc taaagaagcc gaattcttca gctttggaac aaacgactta acgcaaatga
      481 catttggctt tagccgtgat gatgctacga agtttattcc tgactatact gaagcacgga
      541 tcttcgaaca agacccattc caaaccttag acactgaagg cgttggccaa ttagtgcgct
      601 taggtgttga acgcggtcgt aagggcaacc ctgatgttca cttaggaatc tgtggtgagc
      661 atggtggtga tccaaactct gtcgaattct ttgatagcgt tggcttagac tatgtcagct
      721 gttcaccata tcgtgtgcca attgctcgct tagccgcagc ccaagctgca atcaaacata
      781 aaaaagctaa gtaataatta ctattaagat agctttaacc tcgttcttcg gagcgaggtt
      841 ttttattatt taaaaagacg ctcaatacaa gcgtctttcg ttaatttctc agcaatttct
      901 atttcttttt ttgtttttca cgaattattt tcgcatagcc ttctttattt tcttggcgtg
      961 aacgcgcgat agcaagtgtg tcttttggta catctttatt aatgatcgat gcagcggcaa
     1021 gataagagcc ttccccaatt tgaaggggtg ctactagagt cgtgtttgtg ccaataaaga
     1081 cattttcacc cacttttgtt tttgatttat ttacgccatc ataatttgca gtaattgctc
     1141 ctgcaccaac attagtgtgt gcgcctaatt cagcatctcc catatacgct aagtggccag
     1201 ctttaacgcc ttcgtggaat gttgtatttt taagttcgac aaagttccca atatgtgagc
     1261 cgtcttttac caaagctttt tgtcgcattc tcgcaaacgg accaatcgtg acattgttac
     1321 aaatttctga gtctactaag taactataaa taatagaatt ataatcgcct atcttcatat
     1381 tctttaaata tgtgtttggt ccaatttcat ttgcaacacc aatcgtgcta ttgcctaata
     1441 tcgtagtgtt aggactaata attgtatctt ggccaattgt gacgtctggt cctatatatg
     1501 tgttatttgg atcttcaagt gtaacgccgt ttagcatatg tgctttatta atgcgttttt
     1561 taaggagttc cgtagcttcg gcaagttgcg ctctatcatt tgtgccaagc atttcatcag
     1621 gatcacttaa cacaaaggca ccaactttat accctctttc tttaaataaa ccgataatat
     1681 ctgttaaata taattcgcct tgtttattgt ttggttttaa gtttttaagt tcttggaata
     1741 acttttggtt attaacgaca tacatcccag cattaacttc actaatgtcg ccttcattac
     1801 ctgttaagtc tttttgttca acaatgcgag ttacttcacc gtttggtgcg cgtaaaactc
     1861 taccataacc aaaaggatta tctggcttag cggttaatat tgttaaatcg tttttctctt
     1921 tggtgtgaag attaagtagt ccgctgatgg agtgttcagt aataagtggt acgtctccac
     1981 ttaaaattaa agtgtcaccc ttttcctttt caagtagcgg tgctgtttga atgattgcgt
     2041 gtcctgtacc tttttgttca tgttgccaag caatttcagc tttcccttcc acgagtttag
     2101 aagtgtgttc accaccaaaa ccgacaatca caacagttct tcctgttaca tgcggctttg
     2161 cggcatctaa cacccaatta ataagtggct tgcctaatat ttcaaaacct actttcgata
     2221 tttcttgatt taatgatttc atgcgggtgc ctttacccgc tgctagaata atggcatttc
     2281 ttttcataaa attctcctat ctgatgactt ttgtcattac tgtttgataa ttttgtttgc
     2341 gatatttatc actaataaaa cgtaataagt gtcgattatc acttagtgca aaaatcgtag
     2401 tacctgttcc cgctatacca taagcttcaa catttaattg ttttagatca ttaagcactg
     2461 tccgtaatgc tggtgtatgt ttaataatcg tttcactaaa gtcattaaaa agcacagaac
     2521 ctaactcacg caaagaacca ctttctgcaa gttcaacaac tttttcaagc ggtttttgta
     2581 gtgattcacc ataatccaaa aagtctttaa caacattttc tttttcataa atgtctgggt
     2641 ggataattaa taacacctgt ggattatgtt taaattttat aggtgttact tcttgtttgt
     2701 ttaaattgta cactgcgggt ttatttacta aaaaataagg gacatctttt ccaattttga
     2761 ctgcaagatt ataatattgc tctggtgttg gttttagttt agcaaggtgt gcaacagctt
     2821 tcacgacagc ggtcgcatca cttgccccgc tccctaaacc gtgtcctgtt ggaatatttt
     2881 tatggatttt aacagagaag ttttgcttat aaccaaattg ttcccgcatt tttagtaacg
     2941 cttcatcaat aatccgtttt tctttaacat gaagcgcctt attagtgtaa tgaacaatat
     3001 ccataaaggc agtgttgaag acatcaatcg taattgtgtc actaaattca ataggtaaaa
     3061 caaggctttt aatctctaaa gcgttagtca atggattaag cgttgtttct acacttatgg
     3121 acaagcgtgc attcgcagtt actatcatat ttatatgata tcaaaattac attgttttta
     3181 gggcgttttc agtgcttata ataaactaac agtataaatc cctgtgtgta tctcataaga
     3241 aaacactcct ttcctattgt ctaggatttg gggtgcatct caaaattatc ttttttcttt
     3301 tgttaacaat gtagataagt atttgtcctg ataccctaag cgtagacact ttaataatga
     3361 aagaccagga atttgacttc ctggtctgtc caccttggat ttaatagttt tagggtgtta
     3421 atttggtttt ccaccttttt ccaccttaga tttaatagat ccaaataatt taaaaatata
     3481 atggcctttt agctaaatat gagatgttca agttcgcata atcagtatca taattataat
     3541 agtctagttc catggtttca tttgacatca ggttaatata tttatcttct gtttttatcg
     3601 cgggtatata accccctatt tttccgttgt ttgcttttaa aaaatattat ttattgttag
     3661 aagtgtcaca attttgatat ggattaaact gactattttc attattattt tcggtgtgta
     3721 caccaaaact attatttgtg tcacgcaaat atatggttct tttaacactt attatactag
     3781 tgcggtaaac ctttaaaatc ataaaatctt ggggttcaaa ttctacaagt acaaagctaa
     3841 atttattata ttcatcataa agaggaagtg ctcgtaaatt tgtataatcc atttctcgct
     3901 cttctatttt ttgattcact cttttttcaa cttcctttaa atgtttttca ggggaatgaa
     3961 aaaccttgta tggttctttg gctccccatg gtgtaggata aagcgcaaac aaaagagcga
     4021 acataattaa agtaaatggt aatattaacc aaaacaattt ctttttattt ttcattgtcg
     4081 tttcctccct ttttattaac aacagtaatt gtatatttct tcattatttt ttggaatatc
     4141 aagtgtacca ctattaaatg catctaaatc agcgtcactt ataacataga caccgttcga
     4201 taataaataa cttccgttac taaaatattc aacaatgtag ttgaaaattt gcagtatttt
     4261 ttcatgttat attccttaga tttgatattt acatattcaa tatatcataa attgctgaaa
     4321 tttccaattt tccctcatgt ttgagttttt tgttgattgt cattttaacc cttttaatta
     4381 tcgtaatgtt tcaaataatg ttaataaatc gttaggagat agtgcttctg ctcgaacgtt
     4441 ttcgttaatt ctatgtttct ttaaagcggt taatatatct tctttgtcgt atatgttttt
     4501 caaattgttg tatagtgttt tgcgccgatt tagaaaagcg ttatttaaaa acgaataaaa
     4561 ttcacgcaca ggaatatcgg ttttatatcg tgtaaaacaa aataccgctg aatcaacatg
     4621 tggcactgga taaaacttat cagcggttac cactagtttt tgttcaacat caccaagcaa
     4681 actaagtaaa acccgcagcg gactatatgc ttttgttccg ctttttgcta acaacctttc
     4741 tttaacatca atttgtgtca taaatacaaa ctttttaagg ttttctgctt ctaataacac
     4801 cttttctatt atttttgtgg tgatgtaata aggaatatta ctaataacag cctttgtttc
     4861 acgtggaaca ctatattcta agaaatcttt ctcaatgatg ttaaggtgtg gatactttgc
     4921 tcttaatact cgcacaaaac ctttatcaat ttcaattgca gtaaaattta cggtctttaa
     4981 aagcagctct tttgttagtg cacctagtcc aggtccaact tcaattacat caatgtaatt
     5041 aggttcaaga aagctgacaa tcttacgcgc tacattttca tccactaaaa agttttgtcc
     5101 ctttcttttt tgtggcgaca agttttcctt tgctaataat gttatgattt cttctttatt
     5161 cacttagtgc ttcctttaat tctttataac ttatatttaa tagttgtatt tgcgttaaca
     5221 tatttttaag tgacgctact aataaactat atttagtcgc taactttatt tttttaaatt
     5281 gtgaatttgg cccagataac cctaaatcta gtaattttat aagtgtaaca tcggaagaag
     5341 tagtaaatgt ttcttgctta taaggtttaa ttaattccgc tagaagcggt agcggaatat
     5401 gttctattcc aacttttcct ttctttactg cttttttctt tattactttt attactttag
     5461 cgttaggtat ttcttgtaaa agtttgtctg caatcttttg ccctggtccg tctggatctg
     5521 ttaagattat aacctcatga attttcgcta attcttgtaa atgctttgtt gtttcacgtg
     5581 gaacatttag cccgtctgtc cttactatgt aagggatgtc taactttttt agtctttctt
     5641 cgtcgcgctt tccttccgca acaaagatac aatttaacaa tgttttgcta ctcataattt
     5701 aaataatgtt ttagcgttct ttgttgtaaa ctctgcaact ttctcgacag aaacattacg
     5761 aatcgttgct atggtcttag caataagtgg gatatttgca ctttcattta atttgccgcg
     5821 atatggatgc ggggttaagt aaggcgcatc tgtttcaagt agaagtcttt cttcgcttac
     5881 aacctttgcc acttcctttg gctcacgcgc atttaaaaaa gttactggtc ccgctagact
     5941 tatatacatc cccaaatcta taaaacgcaa cgccatttcc tttgaaccac tgtaagaatg
     6001 cattattcct tgataccttg ggatattact ctttaacact tcatatgtat cacttgtagc
     6061 gtcgcgcata tgaataagca ctggtttttt atatttattt gcaagctcaa tttgcgctat
     6121 aaagaattcg tgttcaattg ctctttcttt ttcgtctttt atccagtggt aatctaatcc
     6181 tatttcccct actgctataa ctttgggatg ggtaagaagt ttttcaagtt cttttaaatc
     6241 acttttcggt gttttaacag cgtcaactgg gtgaatacct gcggcagcgt atacattatt
     6301 aaacctttca gcgattttaa ttgctttttt actagatttt acatcccatg cgacaattaa
     6361 aagataatta acgcccactg ctttagcacg ctcaacatac ccttcaatat cgttttcgta
     6421 tcttttatcg tttaaatgac agtgtgtatc aaaaaacatc tttatttacc tacacagaaa
     6481 cgcgaaaaga tttctttact taaatcatta tcaacgtttt ctcctaatat ttctttgatt
     6541 gtaaagaaag cttgttgtaa agacacgcta acaaggtcaa tagcgacttc atcaagcgcc
     6601 tcttgtttag cgatttcaag ttcgccataa gctttgcgca acaacgctag ttgacgcgca
     6661 ttatttaaac ttggtcgtaa ataactatct tcatcaactc ctacaacttt agcaattgct
     6721 ttttctaagg caccaatatc attagtgagc gctgaaatgt aaagtcgatt aggatctttc
     6781 ttacttacta agtctccctt gttataaacg gtaattactt tcttaccttt aattaaacca
     6841 gtaaattcgt cttttttact tacattttct gcgtctttta catatattac taaatcagcg
     6901 tctgcaattg atttctttgt tttttcaata ccgatacttt caattgcatc tgtgctttct
     6961 ctaattcccg cggtatcgag aagaagaagg cgaagtccgt gtaaattaat ttcaccttcc
     7021 acaacatcac gcgttgtacc agcaatatca gtaacaattg ctttatcctc tttaagtaat
     7081 gcatttaata atgacgactt cccaacatta ggagcaccaa caatagcgac tttaattcct
     7141 tctttgatta tttgaccttg tcgtccttga tcaatcaatt tcttaatctc cgcatttata
     7201 taatcaactt cactaataac aagtgcgtag cttgcttctt caatgtcgcg atactcagga
     7261 taatcaatgt taacttcgat taaactcatc acgtccgcga ttctttctct tatcggataa
     7321 acgattttac tcgtttcacc gactagcgat aatagtgaaa gtttctttgc ttcttttgtc
     7381 gtcgcggtaa tcatatcgtt aacagcttcg gcctgcacta aatcgagctt gccgtttaaa
     7441 tatgcgcgat tagtaaactc tccgcgcccc gcaaggcgtg cgccgttttt aatcattaat
     7501 tcaattatct cgttagcgat aagcatgctg ccgtgacaac taatctcgac aacgtcttca
     7561 ccagtaaaag aattcggccc tttaaaacaa aaagcaatta cttcgtcaac aagttcttcg
     7621 tcatcaaaaa gtttgccgta caccatcgtg cttttttcta gtttagttaa atctttcgta
     7681 aaacttttgg taacaatatt aaaagcctca ggtcctgaga gacggatgat ggcaagggcg
     7741 cccatcattg gtggagtagc aagagcaaca atcgtatcta acatagttaa attttatcat
     7801 atcctattat ctaataagaa aaaaccacct tagcggtggt gtttatcttg ttttattcgt
     7861 cttgaggttc ttttggtgcc ttttcttttt tctctggctt tttataacga atgatgactt
     7921 gtctttcttt gcctgcacca attgattcag tggtaacatt gcgatattca cttaatgcat
     7981 tatgaatcgc gcgcctttcg tcgcttggca ttggatcaag tgttgcgtct tgcttagaac
     8041 gtgcgacgtc acccgcaact ttacgtgcta agccagtaac tctacggtaa cggtcatttt
     8101 tataattatt aatatctaat aatactcgaa tgcgtttttt aaatttaacg gtcatcgcga
     8161 cttggacaat ttcgtttaac gcgagtaatg ttttaccacc cttaccaata agaattgaat
     8221 tgtggtttgt gctcatttca attttaatga cttcgccatc taaactcgtg gtgaattcag
     8281 gagtaaaacc taatgactcc acgactaacc gtaaataatt ttcagcgtat tcaatgtaat
     8341 cagttgtttc gtatacactg attttgattg aacttttgaa aagtcctttc ttttcttcaa
     8401 gtacttcata cactaagtct tttgtttgaa taccaatatc ttcacttgcg agcgctaaga
     8461 cttcatcaag gttttttgct tcaaatatcc gcattattgt ttccttcctt tcatcacatt
     8521 atgaacaact acagtttgaa caattgagat aattgcccct acaaaccaat acacacccat
     8581 cgctgcagga agagtaaacg acataaagac aatcataata aacataatat tcatcatcat
     8641 ttttgattgc cgttgttgtt tgtccgcagc cggattagcg gttgttttac caatttcttt
     8701 tgttttgctc ttgttaagcc atgttgataa ttttgtgcta acaaattgtg cagcggtcat
     8761 taatataaat aacacccacg ctgtccacca gccaccctta aaccaaccat taatcattga
     8821 ttgaccaagt tgagcactta agttaagccc taaaacacta tcacttgcta aactagcaga
     8881 accagtcatt gcgccccata ccccaataaa gattgggaac tgaatgaaca ttacaagtag
     8941 tgatccaagt gggttgattc cgtgtttctt atataacgcc atttgtgctt gcgctaaagc
     9001 ttgtctttca tattgattag tgtcagagtt tggatacttt tgttgaatct ttgcaagctc
     9061 tggttgtaag gcttgcatct tttgtgcacc caccgtttgt ttaagtgtca taaggacaag
     9121 tagtccccgc acaataattg ttgtaactaa gatggcccaa atttgtcccc atccaccagc
     9181 gccaaatgat tttgttaaaa attcaagtag tgctgcaaca ggataaacaa ttaagccttc
     9241 aatgaaccct ttcttccatg cacctttcca tgtcttggct tcgactggaa ttttactatc
     9301 ttctggtcca tattcgccgt cataaagcgc aatacaagca cgatttgcct taattttgtt
     9361 ataaatttgt gttttataaa aattagcaaa gtcgcggtcg ggaacatttt cataaccaat
     9421 ttctgcacca atttctttgt tccaaattgt ccagttaacc cacagttcat taccatcagc
     9481 agtcactact gttgcgcctt tggtacctaa aaactttaag taaccatttt gttttaaggc
     9541 cgcagcgtta agtgcggcgc cttctaagtt ttcactatta tcaaaattta gaattgcata
     9601 atctaaaact ttttgatcta acttttcata atactctagg ctaggagtag cgtagccttg
     9661 actttgagca tctaaaataa tcttatgtaa aactttgttg tgtacttttg tttcagtacc
     9721 ttcaacgact tctgtaacta accctgaatc ttgagcataa agcatatagc ccacgtcttt
     9781 cgcggaacag aagctcgcgg tacatgaaga aagcacgaaa agtccggcga caattgcgcc
     9841 tagttttata atccgttttt tattcttttt catgttaatc tccgttactg tcgaatattt
     9901 tcgcaattgc atctactaaa tcaccgaagg atgaatcatt aatttccttt ttcgccacaa
     9961 tgataaagtc accagtatgc gttttaacat acggtctaac aactgctctt atttgtcttt
    10021 ttattttatt gcgggtgacg gcgttaccaa ttttcttgct aacagcgaca cctacacgta
    10081 aagtttcgag ttcgttagaa gcaaagtaac catagcaaag cggggttttt gtgagctttc
    10141 cattttttat aatgttttgg aattgctcgt tttttaaaat ccgatgctcc cgtttcacaa
    10201 ctctcctcaa aagtagtcag cttagaccgt taaagattca cgacctttag cacgtcggtt
    10261 acgaagaact ctgcgaccac ctggagtcgc cattctagcg cggaacccgt gaagacgagc
    10321 ccgtcttaat ttacttggtt gatacgtacg tttcataata tttctcctca ttcaatccaa
    10381 cataaagttg ttcgcgatta attttagtgg tttacatagt aaaagtcaac caatgactat
    10441 taaacacgaa ttagtagatg cttctttcaa caacgaataa taacaggata tcgttaaatc
    10501 tgtcggtctt tttggctttt tttcatcatt ttttattaat taattgaaaa atatccactt
    10561 ttatgtggaa aacacatacg attatgttta attaatcaaa aaaaccgatt atatgaaaca
    10621 aaaacattgt ggacaatttt aaaacactgt gtataactct tgcgcaagtc cacattatca
    10681 acatggaaaa ggttttccac aatgttatcc acaccattat tttgtggatt tggtggataa
    10741 ctacatataa tcgcgctttt ataagtaaca aattgtggac aaaaaaatct tattccctta
    10801 tctctttatt tttatcccaa ttatgttaaa ttattgctaa agattgcgga ggcaaatagt
    10861 ttatgaacat gtcaatttca gaaatcaccc aaatttggga tcgtacgctt atcaagctcc
    10921 aagaaaaaat tcaagatcga agagtttatg gagccttctt ccctgacact tatattcatt
    10981 caattaacaa cggaatcatt acagttatcg ctaattctag cttagctaag tcaatgttaa
    11041 acacgaatta tcgcgatgtt atagaagaaa ctgttggtga aattacgcaa tctaattatc
    11101 aaataagctt tattgaaaaa agcgaaatcc cgcgtgttag tgaaattatt agcgaaaaga
    11161 aagagacatt ttttaaaaac gcgaaagtta attctaatct tacttttgat aattttgttg
    11221 tcggaagctc aagcaacctc gaagcttatc aagcagcggt tgttgtcgct aattcaccag
    11281 gtaaattata taacccgctt ttcctttata gtgcctcggg tttaggaaaa acgcaccttt
    11341 tacatgcgat tggtaatcat gttaagcaaa attccccaaa tcttaaggtt ttatacatta
    11401 ccgctgatga gtttactgat gaatttattc gttttgttcg tgataatccg ggcggttcgc
    11461 tcaaagactt tttccgtact gttgatgttt tattaatgga tgatgtccaa tttttagcgg
    11521 ataaaaagtc gacaagtgaa acatttttcc atctttttaa cttattggtc ggctctggta
    11581 aacaaattgt cttaactagc gaccgtcacc caagcgaact taaaggtatt gaagagcgtt
    11641 tagtgacacg ctttaactct ggtttacaaa ttagtatctc cgctcctgac ttagaaacaa
    11701 gaaaagatat tttacgaatg aaaatatcct taaataattt agatattgaa gattttgatg
    11761 aagaagttat tactttcttt gccgaaaact ttacaaataa tgtgcgtgaa ctcgaaggtg
    11821 cgctcaatcg tttactattt tatgtagtaa gcatcgcccc aacaaaacac gttacgatgg
    11881 aagtagcact caatagtgtg cagtctttaa tcaatgttaa agacgctgtc acgaaattaa
    11941 gtgaaacaaa aattattaat gttgtcgctg actactataa ccttactcca gcgcttatta
    12001 ttggtcgtaa tcgcacaagc caagttgcgc ttgctcgcca tatcggaatg tacttaactc
    12061 gcgagatttt agaccttccg ttcgcgcgca tcggcaaagc gtttggggga aaagatcata
    12121 gtactgtgat taacgctatt acaaaagtgg aaaaacagtt gaaagaagat cctgctttaa
    12181 ttcaagctat aaacgaaata aaagagcgtt tacaatagat ttatataata aagtatttgc
    12241 ttctgtgcta aaattaaaaa gagtcaataa gcttttttgt ggagttatcc acagtctcca
    12301 cagcccttat tattatcatt aaatatgaaa ggaattaaat attattatga agtttaagat
    12361 caatcgagaa caatttctaa aaggtttatc gatagcctct agaccaactc aagcaaagtc
    12421 tcctattccc attttaggaa acattttatt gtcattagat gaaactggat taacattact
    12481 tggttcaagc aacgaactag cgatccgcac gcatattcca atgcgagtta atgatctcga
    12541 agtcattcgg atgttcttac caggtgaaac acttatttcc agtaaaacac ttaatgaatc
    12601 aataagaaaa atggaaggga acgaaattga tgtcgagatt attgaccgta ccatcgcaca
    12661 aattagtgac ggtcgcacag aatatcgact cggcgcgatt gattctaaag actatccaga
    12721 tattgattta agtcaagatg gcaccgtatt tgaaattggc gctaaagatt ttattaaact
    12781 cgttgatcaa acttcatttg ccgctagtgt gagagaacaa aggccaatct taaccgcatt
    12841 aaatttaaat gcagttaatg gtactttaac ggcagttgca acagacagtg caagactagc
    12901 aaacaaagtt gtgacattaa aagacaatgt ttcgtttgtc gctaatgttc cagcaagaat
    12961 tatgaacgaa gtcgctaagc ttcttgaaac aatggttggc attgaaacaa tcgaagttag
    13021 tgcgtccgcc aataaaatta atttcatgtt tgatgataca ctcgttgtct cgcggcttgt
    13081 cgctggtgaa tatccaccaa ctcgtagcat tatcccaaaa acacacgcta gtttcctaga
    13141 agctaacgct aatgaactct taaaagcgat ggaccgtgtt tcaattcttt acgcagcgga
    13201 aaaagataat atcgttaaac tgtcattaag tccagaaagt gttgttgtct ctgcgcgttc
    13261 aacattcagc gcggaaagtg cacgcgaaga agtagcgctt tgtaagtatg tcggtgaacc
    13321 acttgaaatt tcatttaact atacattagt aagctctgca attaaagcga ccggtgctga
    13381 agatgtcacc atcgggttta acggcggtca caaaccattt agcatcagag tagcgacaga
    13441 tgactcgctt gtgttaattg ttacaccagt tcggacataa ttaaaataga gcaatttaga
    13501 gcgaaaatta ataaaaaacg ggcaaatcct cacgattcaa gcaaaagtcg ttctaaggac
    13561 acaaaaagga gcaaatttaa gggaatattt atattccctt ttcttttttg tggtataata
    13621 cgttcatagc gacaaggaga gcgtataagt ggaaacaaaa ccatatataa ctttacagca
    13681 agcactaaag aaatataatc tcgtttcaac aggcggagaa gcgaagtttg taattcaaaa
    13741 cggcgaagta aaagttgacg gaatccttga aactagacgg ggtcgtaagc tttatggcgg
    13801 agagataata acttacgcag cacaaaaggt agtagtgaaa gatgttgatt agcgatcttg
    13861 aactattaaa ctatcgcaac tacccaaagc ttaatgtcat cttcaccagt ggactaaacg
    13921 tcatcgttgg tgataatggt gtcggtaaga caaatgttgt agaagcgatt catctctttg
    13981 cgttcgctaa aagctttcga acaaacgata caaaagcact aataaagcac gataccgcaa
    14041 aagcgattgt aagcggaaca attcatatac caagtcggac agatataagt attgagttaa
    14101 caaaccgcag caaaaggata ctaataaatg ggaaagtgct cccgaaggtg tcgcaattaa
    14161 gtaagtatgt tcggacgaca accttcgaac cagaagatgt actcttcttc gacgcaagtc
    14221 ccgctaaaag acgaaagttc ttagacgcaa acttaagtag cgctgatgaa gtttatcttg
    14281 cgacattagt gcgttatgaa aagttgctta aagaaagaaa cgtgctccta aaaacggatg
    14341 ataacgatga agcactcgac ttaatcgata aactattcat cgaactagca aaaacgattg
    14401 tagaaaaacg cacaaggttc atccgtgagt taaacaaagt agttaatgaa gtactcttga
    14461 agttaagtga tgaacaccta agcgtaaggc taacatatga atcttcgctt ggtagtgtcc
    14521 aaaacattga gagaaacgga ttaaaacgac tagcagaagt tagagcaaaa gaaaacattc
    14581 tcgaaagtac aacactagga ccgcatcgtg acgacttaat agcaacaatt aatggtcacg
    14641 gtgtaaaaga acacgcatca caaggccaaa aaagattaat tgttatcgcc ttagccctag
    14701 cgccttactt cttagagaag gacttacaca aaaaaccgat tattattctt gatgatgtct
    14761 taagtgaatt agataagcgt catcaagaac gattaataga gttattactc acatgcactc
    14821 aagcatttat taccgcaaca cattatgaca atcacgctca tgcaatttat gaagttacaa
    14881 gtgaccgaga actaaggagg attacctaat gagtgaagag aaaaaacata aaatagtaga
    14941 tgaagaacta gagcaagcta aacaagaaat gcgcgctcgt gaaagttaca ctgctgaaga
    15001 cattcaagta ttagaagggt tgcaagctgt acgacaaaga cccgggatgt acattgggac
    15061 aacaggactt cctggactcc accatttagt gtgggaaatc gttgataacg ctgttgacga
    15121 agcactagca ggcttttgta ccacaattga tgtctcaatt aacaaagacg gttcggtaac
    15181 agtagcggat aacggccgcg gaatgccggt tggtattgtc tcacaaactg gaaaaacagc
    15241 tgtagaaact atctttactg tattacacgc tggcggtaaa ttcgggaaag gcgcttacca
    15301 agtagcaggt ggtctccacg gtgttggtgc ggcagtcgtt aatgccctta gtgaaagagt
    15361 tgaagttaat gtctataaag acggagatga atactactta gcgtttaata acgatggtca
    15421 cactgaaggc gaattaaaaa aggttggtcc cacaaacaaa cgcggtacaa gagtaacatt
    15481 taaacctgat ccacttatct ttgtcgaatc agtggaattt gattacaaaa caatcagcac
    15541 gcgactaaga caaatggctt atttaaatcg cgggattaga ataaatgttg aagatcgccg
    15601 tggcgaagag cttaaacgcg aagaatttta ttacgaaggc ggtattgcag aatacgtcca
    15661 acacattaat aaaaatagaa caccgattac tcaagatgtt atttttgttg agggcaaagg
    15721 tgatgtcgaa attaacggac aaaaaagcca catcttagtg gaagtagcga ttcagtacac
    15781 gactgaatat aaagaaacga ttttctcgtt cgctaataac attcaaactg gggacggcgg
    15841 aacacatgaa caaggtttcc gtcttgcggt cggccgtgta tttaacaatt atgcgaaaga
    15901 agcaaaaatt cataaaaatg aagacgaccg ttttacttat gaagacgtcg ttgaaggaat
    15961 gacggcgatt atttccgtta aacacccaaa cccacaattt gaaggacaaa caaaaggaaa
    16021 gctcggtaac actgatactc gggctgttgt cagtcaagtg ttaggggaaa aactcgaaga
    16081 gtacttgctt gaaaatccaa atgaagcgaa acttatttta gagaagattt cactttcatc
    16141 taaagcgcgc cgcgctgcaa cattagcgcg tgaaggtgtg cgtcgtaaag acgggattga
    16201 atttacaacc ttgccaggaa aactagcaga ttgtagtagt cgtaatcctg aagaaagcga
    16261 actatttatc gttgaaggta atagtgcggg cggtagtgct aaaaacggtc gcaaccgtca
    16321 tacacaagcg atcttgccgt tacgcggaaa aatcttaaat gtgcaaaaag cgcaagaaac
    16381 aagaatctat gaaaacgctg aaattgggaa catgattcaa gcaattggtg caggaattgc
    16441 cgaagaattc gacataacta aagcacggta tcataaaatt attattatga cagacgcgga
    16501 cgttgatggt agtcatattc gcgtattact attaacattc tttaatcgtt ttatgcgtcc
    16561 acttattcaa aatggttatg tatacattgc ccaaccaccg ctttataaag tagattatcg
    16621 cggtaaacaa tattattgtt ataacgacga acaattagaa aagcttaaag ttgatttaga
    16681 tctccgtcca ggttatcctt tccaacgcta taaagggtta ggggaaatgg acgcacatca
    16741 attatgggaa acaacaatgg atcccgcaaa cagaaaactt ttaagagtac acattgaaga
    16801 cgcaatcgaa gctgatcaag tgtttgaaga tttaatggga gaaaaggttg aaccacgcaa
    16861 agagtttatt aatgaaaacg ccctctttgt gaaaaacctt gacgtataag gaggacatca
    16921 tggtagaaga caatatcaaa gatatgctcg aagaagaaga gaagcatgaa ttagaaggca
    16981 ttgacaccgg tatacacccg gttgatgtcg tagatgaagt tcgtaaatca tttttagatt
    17041 atgcaatgtc ggtaattgtt agtcgcgcta ttccagatgt tagagacggg ttcaaaccag
    17101 tacatcgtcg gatagtttac gggatgtatg aatcaggaat gacgccagac aaaccgttta
    17161 agaaaagcgc acggattgtt ggggatgtca tgggtaaata tcaccctcat ggtgaccaag
    17221 caatttattc aacattagtc aggatggcgc aaccatttag tttacgttat ccgcttgtgg
    17281 acggacacgg taacttcggt agtattgatg gtgacgaagc agcagcgatg cgttataccg
    17341 aagcacggat gggaaaactt gcaacggaaa tggtgcgtga tattaattta aacactgttg
    17401 attttggtgg taactatgat ggtagtgaaa aagaaccaga agtattacca tcacgcttcc
    17461 caaacttaat tgttaatggt agtgacggga ttgctgttgg gatggcaaca tatatggtgc
    17521 cgcacaattt aagtgaaaca attgatgcga ttaacctagt agcgaaaaat cctgatgtta
    17581 caccacgcga aatttttgaa aacggaatgc taggtccaga cttcccaact ggtgcgagca
    17641 ttttaggtcg caaagggatt ttagattatt ttgaaactgg tgaaggcagt atcattgttc
    17701 gcgctaagac gcactttgaa acaaaaggtg atcggacatg gatcgtcatt gatgaaatgc
    17761 cttataaatt aaataaagca acaacaattg aagcaattgc ccttttagtg cgtaataaag
    17821 aaattgacgg cattactgat attcgtgacg aatcaaacaa agaaggaatt cgtgtggtta
    17881 tcgaattacg tcgtgatgtc gtaccagaag tcgtgttaaa caatttatta aaacaaacac
    17941 gtttacaaac ttcatataaa ataacgaatt tatgtttagt taataaagct ccgcgtattt
    18001 taaacatgaa ggaattaatt gttgaatatt taaacttcca agtggaaatt attcgtcgtc
    18061 gtacagaaca tcaactttcg caagcagaag cgcgcgacca cattgtggaa ggattactaa
    18121 aagccactga taacattgat gaaattgtat caattattaa aaatagtgaa tctggtgaag
    18181 aagcgagtca aaagcttatg gaacgcttcc tctttagcga aatccaaaca cgcgaagttt
    18241 taggaatgac actccgtcgc ttaactggtt tagaaattga caaattaaat aaagaacgcg
    18301 aacaactact tgcgaacatt gaacgctatc gcttaatttt aagtgcacgt gaccatatgg
    18361 ttgatgttgt ccttgaagag ttagaagaaa taaaagctaa gtatggcgat gaacgccgta
    18421 ctgaaattag tgatgacctt gctgatattg ataacgaaga cttaattcca gttgaagata
    18481 tcgttgtgat gctcacaaac caaggctata ttaaacgcgt tgatcctgaa gaatttagaa
    18541 cgattaaacg cggtggcgtt ggtgtaaaag gaatgcgcac aaaagaagaa gaaataattg
    18601 acattatctt acacacaaat acacacactg atgtcttatt ctttacaagc aaagggaaag
    18661 tatatcgtaa acgcggttat caaatcccgc aatatcgtcg tgaagggaaa ggcttaccag
    18721 tcattaatct tttaaatatt gaaaaagaag aagcggtacg tgctattgtg tcgactgata
    18781 catatggtga agaccattac ctcttatatg taacccgcaa cggtgtgaca aaacgtacaa
    18841 ctttagcgga attctcacgc attaatgtca gtggtaaaat cgctattgat ttacgcgaag
    18901 gcgatgaatt aatggatgtc aaaattactg atggtacggc aataattggt atcggttcaa
    18961 gtaatggtaa aatggcaagt ttctatgaaa cggatgtgcg ggcaatgggc agaactgcgg
    19021 ccggagtccg tggtattaaa cttgacgaag gtgcttatgt tgtcggcgtt acaacaagtt
    19081 tagagggtgc tttaatcttt gcgcttacaa caaaaggtta tggtaaatta acacctgcta
    19141 acgattaccg tttaacaaaa cgaggttcaa aaggtgtaat taccattaaa gaaagtgaac
    19201 gtactggaac acttgcggga attagagcaa ttaagggaga tgaagactta tttgttacga
    19261 ccgctagcgg aatggttgtg cgcaccaact ttaaagaaat tagcacaaca agccgaaata
    19321 caatgggtgt gcgtgtcatt cgtttacgcc cagatgatgt tgtttgttct ttcgcagttg
    19381 tcgaaaaagg gattgctttt gacgaagatg atcacgaagg tgaagggact cctagtcacg
    19441 aagaatagtt atgaaaatat taatctcttt tccacataaa aagattgatg tcttctttga
    19501 aggagcaatg cttgaatatc aaataaaaaa agcatgccaa agagatgatg taacacttgt
    19561 tactaaattt agtcctgata ttgatatcgc taattttata aatttgaata gtcaatcttc
    19621 gaaagtgatt aggcaatcaa ttgccgcaag tatacccacg cttttatgga tgttcttcgc
    19681 taataacgac acttacgctc gtgtgattga aacaagaaaa gatggagagt tatacattcc
    19741 tttatcgcgt cttgaattaa ttaatatgat ggacggtatt gttgttccag cacaagaagc
    19801 gcgtgttatt ttacgaaaac tcggtgtaaa aattccaatc tttgtaattt atggagcggt
    19861 tgatgttcga cggattgatg agttaaaaac aagcaaaaca gatgtatttc gtcgctattt
    19921 ccgtataaac gatgatcaaa aatatgccat aacagtgatg aacattaaag caagtgaaca
    19981 tattgaccaa cttaatgact tagccgctgc tgtaccaaac tataatttct atgttttcat
    20041 cagcggtggc acatcattta ttgatagatt gcggcttaaa gcgatggata aattaacttc
    20101 accgaactta attgtgacta agcttgtacc agaagatgtt tatcggatgg gtgttattgg
    20161 tgcaagttac ttcattgatt taggttacga caagatgaat gtaatgacgc tttttgagcc
    20221 gatgtactta aaagtaccac ttattttacg gaaaaatgta atttttaaag aaattgttga
    20281 taagacaaaa gcttacattg taaatgacta taacggagcg gcttacttaa ttagaacaga
    20341 agcagaaaca gataaaatta cggaaaaagc ttacgaatat acacaaaatt ataacgccgc
    20401 tttatttaca gaagcgattt ataacttgtt ttataagata tatactcgct aataaagtaa
    20461 aacttgataa aataaatgtg ttcagtgaag agactagtaa taattcgcgg tttaacagtg
    20521 actcgctgat agtgggaagg cgagaactaa aggttatgaa ttccacctcg gagaacaaag
    20581 cttaatcgct tcgtttgtcg cgttaagaca ataatgagaa tacaactttg tatcaaactt
    20641 aggtggcacc acgttaaaga cgtcctaatt agggcgtttt attttgttta agaaaggagt
    20701 gcgtattatg ttagatatta atttaattag aaatgaagca gaaaaagtta aaagtttact
    20761 ttttaaaaaa gggtgggatt ttgatcctga accaatttta agtcgtgatg aaaaacggcg
    20821 gaaacttcgc gttgaagttg aagaagctaa agcaaaactc aacaaattaa gcgcaagtgt
    20881 accggttgtt aaaaaggctg gaggcgacat tcaggccatt tttgctgagg taaaagacct
    20941 gaaagcggaa aacgaagcta aggaaaaaga attaaagaaa cttgatgaag aagtttttaa
    21001 tttactagca gaattaccaa atttaccaga cgaagactta cttgcgggtg gaaaagaaaa
    21061 taataaagtt gtgtatactt ttggtaaaat gccaaagttt acttttccga ttaaagatca
    21121 tgttgaactt tgtgaaagtt taggacttat cgattataaa agagctgcaa aaatttcagg
    21181 aagcggcacg tggatttaca ctaaagcagg agcactactt gaatggggac ttttaaactt
    21241 ttttgttagt gaacacttaa aagcagggta cgacatgatt ttacctccgc acttattaaa
    21301 ttatgaaagt ggctttaccg ctggacaatt tcctaaattt aaagaagatg tgttttggtt
    21361 agaaggaaca gaaccgcgta aattcttact accaacttcg gaaacagcac tcgttaattt
    21421 ttatcgtgac gaaatattaa aagaagatga attaccaaag aaatactttg cctacactcc
    21481 gtgttatcgt agcgaagcag gtagttatcg cactgaagaa cgcgggatga ttcgtggtta
    21541 tcaatttaat aaagttgaaa tattccaata cactaaagag caagattcat caaaagcttt
    21601 cgaagaaatg gtcgctcacg cagcaggtct tgtcgagaag ttaggcttac acttccaaat
    21661 tagtaaactt gcggcaggtg actgttcgca ttcaatggca agaacttatg atattgaagt
    21721 atatattccg tcaatgggta tttataaaga agttagcagt gttagtaacg ctagagatta
    21781 tcaagctagg cgcggtatgg cgcgttatcg cgataaagaa acaaatgcgg tgaaatattt
    21841 acatacactt aacggttctg gactagcaac atcaagatta ataccagcaa ttgttgaaca
    21901 attccaacaa gcagacggca ctgttttagt tcctgaagtt ttacaagcgt ttgtggggac
    21961 aaaagtttta catcctgact ggtagttgct ataatgaagg tgccatcgcg atggttattc
    22021 ttcgaaccag gtcaggacgg gaacgtagca gccttaagat tacttaatca tgtgcggtgg
    22081 tttttaattt aaactatgaa taaagacgaa aaatatatga agctagcgct taaagaagcg
    22141 cacaaagcag cggcaattga tgaagtacct gttggtgcag ttatcgtgct taacgataaa
    22201 gttattgccc gaggttttaa taaacgcgaa attaataacg acccgcttgg tcacgcggaa
    22261 atgattgcga ttcgtaaagc gagtaaaaaa ctcaacaatt ggcgacttgt cgactgtgaa
    22321 ctctatgtca cagttgaacc gtgcgcaatg tgtgcgggcg cgataatgtg gagtcgctta
    22381 aaacgggtag tgtacggcac tggtgatcca aaaggcggcg ccatgggtga agcgtttaat
    22441 ctttttgatg agcgaatagt taaccatata ccggaaataa aaggcggggt gctcgctaaa
    22501 gagtgtaagg aagtgattca atcatttttc cgtaacaaac gaaaaatata atatgtatat
    22561 aaataaatgt ttacatacga taattgaata atcataatgt gcgctttcta ttatttgaca
    22621 caggagtagt tttgaaatat agtttaatta tgcaaaaaag aatccttatt gtaatttcct
    22681 tactaattag tgttgttctt aacggctgta caaaaccaca aaaagaacaa agtgaactag
    22741 aaaaattcct aattaaatat aaagaagtgt atccagacgg atataatttt caaacaggat
    22801 tttatagtat tacatcattt acaaaatcga aggttgatag ctctgattat tttgaaattg
    22861 aaacatataa cattaaggct caactgcaat caataccgta cggtgtatta tggaattttg
    22921 aagcaaatat gatgcagttg aatcgttctt tagtgttggg tgcaggaaga gaaggcccta
    22981 ttgaaaattt tgaaaacata ttatgggacg gagaatattt gcataaaaca ataaaatgca
    23041 acaattatat acataaagaa acatggactg aaaaaacata tggcacagat caagtagaga
    23101 cattactaat aaatactaaa ttatatacaa tagaatatgt tatacgtatt cttaagacta
    23161 agctgtctta ttatgaaaaa tatgtttttg ataaatcaaa tgttgatttt ataaacaatt
    23221 cgttgattgt ttctacgttt ttcagtgaat ttgaaatata taaaattata caaattgatt
    23281 tcgatgaaaa ttttgtaatt tcacgatatc aattttcata tattgactta ggtagagttc
    23341 ctgatgaatt tagaccaaga caaataacac aaacgctgaa accgatagat gcaattgaag
    23401 ttaacgctca attagaatat gaagaagaac ttatcgtgga aaaagatata tttacgattc
    23461 aaatttagtt ttagcaaagg agattaatca tatgcttaca actttgggac tagtattaac
    23521 gacatgttta tcatttggaa acattaaaac tgaagacata tattatcaac ttccccacta
    23581 acggtactaa atgttttaga tattatatga cttctgaagc agtcggcagt agaaataaag
    23641 gaagaattag cgttggaacc accagaattt atgttgataa gatttagtaa aactaattta
    23701 gaatatttaa aaactaagat taatgtgcaa tttattgtca tgttttatat tatttgacac
    23761 aacgcaggaa aagaggttgt attaacattg gttgaaaaag cacaacaaca atttgaacaa
    23821 aagacaaaaa acaacgcgca agattacgga aggagatttt gatttaaatg atagatacgc
    23881 ttgaacaaat gaaacaaatg cagggatata aaaaaggtgg gccaatgccg tcaggattcc
    23941 caaggatgag gaggtaagaa aattcttatg cgaaaaagaa ttcttgttat aatttcatta
    24001 ctttttagtg ttgttcttaa cggttgtgca aataaagaaa aagaaaaaag cgaattagaa
    24061 aaattcctga ttaaatataa agaagtgtat ccagacggat ataactttca aacaggattt
    24121 tatagtataa catcatttaa aaaatcaaag gttgaaaaca cagattattt tcatatagaa
    24181 acatataata ttaaggctca actgcaatca aaaccatacg gtacattatg gaattttgaa
    24241 gcaaatatga tgcagttaaa tcattcttta gtgggcggag taggaacaga aattcctaac
    24301 gaaaattttg aaaacatatt atgggacgga gaatatttgc ataaaacaat aaaatataac
    24361 aacggttatg tacctaaaga aacatggact gaaaaaacat atggcacaaa tcaaatagag
    24421 acattacaaa taaatactga aatagataaa attgattata ttataagtac tcttgagact
    24481 aagctgactg atttagaaaa acatgttgtt aataaatcaa atgttaattt taaaaacaat
    24541 tcgttgattg tttctacatt tttcagtaaa ttagaaacat ataaaattat acgaattgat
    24601 ttcgatgaaa attttgtaat ttcacgatat caaatttcat atactgactt aggtagagtt
    24661 cctgatgaat taagactaag acaaataaca caaatactga aaccaataga tgcaattgaa
    24721 gttaacgctc aattagaata tgaagaagaa cttatcgtgg aaaaagatat atttacgatt
    24781 caaatttagt atagtaacaa agattaacta aattcccgtc tcttgtaaaa ccgcaattat
    24841 aaaattaatg taaataataa tgcctttatc ttgaaaagat aaataaaaag ttagacaatg
    24901 tataaagtga aatccgaaag gggatattta tgcttgttct caaagatatt tataaagatt
    24961 attatgtggc taaaaagcca cttcatgttt taaaggggct aaaccttact tttcctaaac
    25021 aacaattcgt aacaatttta ggtccatcgg gttgtggtaa aacaacactt ttaaacttga
    25081 ttggcggtct tgataaatat acatcaggcg atttaatcat tgaaggtaaa tcaacaaaag
    25141 aatttaaaga tagagattgg gacgcttatc gtaatcatcg tgttggattt gtgtttcaaa
    25201 gttataatct tatcccgcac ttaagtgtga ttgaaaatgt tgagatttct ttaacgcttt
    25261 ctgggatggg gagtagtgaa cgtcgcgaaa aggctaaaca agccctcctt agtgttgatc
    25321 ttgaaagtga attatataaa agaccaaatc aactttctgg tggtcaactt caaagagtag
    25381 cgattgcacg tgcacttgtt aatgatcctt ccatcatttt agcggatgaa cccaccggag
    25441 cgttagatag tgaaacaagc gaacaagtca tgaaaatttt aaaagaaatt agtaaaacac
    25501 gacttgttgt aatggttacg cacaatgaag acatcgctaa acgatatagc gatcgagtaa
    25561 ttaggatgtt agatggcaga attactagtg acactgatga agcacaaagt aaaaagaagc
    25621 gcaaagtaaa agaaccaacg gaaacagaaa aaaacaaggg aacatcaatg tcctttttaa
    25681 ccgcgcttaa aaacagttat aaaaacatct taacaaaaaa aggaagaaca gtgtttactg
    25741 cgcttgcagg atcattaggg attattggta ttgggttagt agcagcggtt agtaacggat
    25801 ttcaaaatta tattaatcgt ttggagcggg aaacaatggc aaattaccca attgccgttt
    25861 ttgcgcaaac aatggatatg caagcaggca taaaaaaacc tgataaaaac actgagtatc
    25921 cagacggtga agaaatcgtt gtttataatg aaaatgatac agaaacaaca cggattacca
    25981 ttacaaataa taggataaca tctgaattct tagatttagt ggacggatta aaggaagacg
    26041 ggcttgccgc tagtacaatg gttaattatc caacacaaat gcaaatttta gcaaagccgc
    26101 ccactgataa cgcgcgtact gtacagtggc cttattatgt cagtgtgacg ccactaccct
    26161 ttggggtatt tacccaatta ccaggggacg aagcctatat tactgaattt tatgatgtaa
    26221 ttggtaaaga ctcaaagttt gcgacagagc gtaatgaaat tatgtttgta cttgataaat
    26281 ataatcgtat ctcagtccaa gctttagaac ggatgggcat tgttgataat tccgcaaatg
    26341 tacaaaagct taattttgat gacattattg ggcgagaatt taaaatggtg cacaacgatg
    26401 atatttataa aaaggcattg ggtaatattg atgactatgt agtgcttgac tttatggggc
    26461 aaccacgcac gattgaaaag tatcgttttc aaaacccaca atatttatat gatgacccaa
    26521 cggttggtga aatgctaaaa attactggca ttttgcggcc aaaaccggga attcagtttg
    26581 aaatgctcgc gccaggaatg gtgtatacac aagcattaaa taaatatatg catgaaacaa
    26641 ataaacataa caaagttgca aaggcacaag ttaataatgt agtaatcaga gaagcaaata
    26701 tcactgcaga aaatattaat actgcaattg aattttatag cgctactacc gattggtcgg
    26761 gtgtaaaacc agttaatcca acaacaataa ggattgataa atttttaagc aacctcaaaa
    26821 tttatggagt ggacttagcg gatataagta aggacaacgc gatttacgaa acactattaa
    26881 aagaattatt aaatacagct tattcaccag tagaatcagt agcaattttt gctaaagacg
    26941 gtgattcaaa aaaagaaatt aaagcacgga ttaatgaata taataatacg cgcgaagacg
    27001 agaaagataa aattgtaatc tttgatgcag tagagtttgt tactgattca attgcgacaa
    27061 tgattgatgt tgtttcaatt gtattaattg tatttacaag tatctcactt gttgtgtcag
    27121 gattcttaat tggcattatt acttatgtta gtgtgattga aaggacaaaa gaaattggga
    27181 tactgcgtgc gattggggcc aggaagaaag atatatcgag gctatttaac gcggaaacgt
    27241 ttattattgg actacttgcg ggggtaattg gcgttgggac aacatatata ttatcaatac
    27301 cgataaatgt tattcttaac aatctatttc ctgaacaaaa cattggacaa atagcgtttt
    27361 tagtaccact tgcagcttta gcgcttattg tatttaatat attattaacc gtcaccgctg
    27421 gattaattcc aagtaagatg gcggcaaaca aagatccagt cgtagcgctg cgcactgagt
    27481 aatgaataga aaggactcat tatggaggaa tttgtagaaa caaagaaaaa aagaccgggt
    27541 acggcaacaa caatcattaa aaaaagcgaa attaaagttg atgaagtaat tcgttataca
    27601 ccaaaacacg atgaaggttt aagtacccaa caagttaacg cccgcaaagt agcgaaaatg
    27661 actaacgaaa aaccaaataa aggcgttaag tcaatctttt caatattaat gcgtaatttc
    27721 ttttcgttct ttaatgtttt gctctacgca ataggagcag cgcttattta tattaaagag
    27781 tggcaaagtt tgttatttct cgtcattctt ttttccaaca ttttaatcgg acttatccaa
    27841 gactttaggg cgcgacgcac actaaagaaa ctcttaatat taaatgaaca aaaagtaacg
    27901 gtcgtgcgcg atgggattga gttattaatt ccaagtgagg atgttgtact tgatgatatc
    27961 gttaaattaa aattaggtga tcaaatcgca gcagatggtg tggtagtgca cggtagtgtt
    28021 ggtgttaatg aatcactatt aactggtgaa ccaaacatta ttcataaagc aaaagatgac
    28081 cgcgttttgt cggggtcttt tgtcacaaca ggcacatgtt actatcaagt tgatacaatt
    28141 ggaaaattag gttatgcaca aaaattacaa ttacgggcga aagtatttaa gcgtccaaca
    28201 tctgaacttt taaattcaat taattcacta tttaaagtaa ttgggtttat tgtgattgct
    28261 atggcattac tattagtgtt taatgccttt tatcaaggct caacatttga agatagtgtg
    28321 cgctctattg ccggttcact tgtggcaatg attccttctg ggatgtattt attaacaagt
    28381 atgacgcttg ccgtaggagt tattagactc gggacaaaaa acacacttgt gaacgaaatg
    28441 tattcgattg aaatgttagc aagagttaat gtactctgtt tagataaaac aggcacgatt
    28501 actgacggca caatgaaagt aaatgaagtt gtgccactta aaggcgcaaa agacattgat
    28561 ttaaacatta ttgttgcctc aattttaaga gccactaaag atgaaaacgc cactgctata
    28621 gcactcaaaa accattttaa aacgagaaaa tatatggaat acaccaaagt gttaccgttt
    28681 gatagcgaac taaaatgtag cggtgcaatt atggaaggta aaacttatat cattggtgct
    28741 tcaagtatgg tgcttggtcc aaaagcacaa aaacaagttg aagaagttga acgcatttac
    28801 ttagagcgcg gcttacgtgt attagtactt gcttcgtctc gcaagccgtt tatcgctaaa
    28861 aaactaccaa ttgatatgac ccctttagca cttattgtta ttcaagataa tattcgcgct
    28921 gaagctagtg aagtcattaa actattccaa gatgctggtg ttgaagtaaa aattatttcc
    28981 ggagacgatc ctgttagcgt gagtgaaata gcgcgtcaag tcgggattaa taatgccgaa
    29041 aatttattag acttttctaa aattgatgat ccaagtcacc actcacttga agactttaca
    29101 atttacggca gagtgcgtcc tgaacaaaaa gaaggtattg taactaaatt acaaagtgac
    29161 ggtaaagttg tagcgatggt tggggacggc gtaaatgatg tcttagcatt aaaagctgcg
    29221 cagtgttcaa ttgcaatggg cgctggtagt ccagcagcac gcggagtcag tcacttagtg
    29281 ctccttgata ataactttga tgttttgcct tcaattgttg atgaaggacg aagagcaatt
    29341 aataacttac aaaagacatg gtcattattc cttgttaaaa caatcttcgc gattatcttc
    29401 tcgtttatgt ttgttactgc gggcctttta ggaccaaaag gatcaggcat tagatatccc
    29461 tttgaaccaa aacacttgta tttgtgggaa ttattatcat taggaattcc cgccttcttc
    29521 ttatcgatgc aaccgaataa agaaagagtg cgcgggacat ttatggataa tgtcattagt
    29581 cgcagcgtgc cagcaggatt gttgatggtc gctggagcat ttttactcta tataagtaaa
    29641 aactttggcg aaatgtttgg tgcagaaata agtgatacat caatggaaat gattacaatg
    29701 tcgattctta caattacatt cttgtcattt gttattttat atcgaaactc attaccattt
    29761 aatcgttatc gactgacact tttcttagta ctaagccttt tagcaattgg tgttattatt
    29821 ctcgataaca ctgtcgggtt aacatttacg gaaaaatatt tcttagaaat aaacatctca
    29881 agtttagagc caattcacta tgcaatatta actccaataa atttaatact tacaataatg
    29941 tacatattag tggataataa aattgtaaag aaattaagga gagaataatt tatgaaaata
    30001 cggattagcc aagaagtagc ctacgcttta gcgaacaact taccagttgt agcgctagaa
    30061 tcaacgatta ttagtcacgg gatgccttac ccgcaaaatg tggaaatagc gcttaaggtt
    30121 gaagaaattg tgcgcgctaa tggggcgcta ccagcaacaa tagcgattat tggtggtgac
    30181 gttgtcgttg gtttggatga agatgaaatt gaagagttag gccaaagaaa agatgtcatt
    30241 aaagtttcac ggcgtgacct agcctatgtt atggccacaa agaagtgggg tgcgacgacc
    30301 gttgctgcga ccatggttat tgctgctaaa gttggcatta aagtatttgt aactggtggc
    30361 gttggcggag tgcatcgcgg tgcgactgaa acactagata tttctagcga ccttgatgaa
    30421 cttgccagaa caaacattgc ggtcgtatgc gctggtccaa aagcaattct tgatattgaa
    30481 aaaacattgg aatatttaga aactaaaggt gttccagttt taggttataa aaccgacaaa
    30541 atgccagtat tttacacaac aagtccatca ttaaatgtct tatatcgcgt tgattcttct
    30601 agcgaaattg cttcgattgt taacgcaagt catgccttag ggcttgaaag cggaacactt
    30661 gttgttaacc caattccaaa agaagaagcc ttgccttata aagacattga aaaagtaatt
    30721 gatgaagcgc ttgaagaaat gaaaaaagct ggggttaaag gtaaagaaga aacgccttac
    30781 cttctttcca aactaaacga attaacaaaa ggtaagagtc aagccgcaaa cgaagcactt
    30841 gtcattaata acgctaagtt aggagcgtca attgctaaac atatttgccg ggagttatat
    30901 gaagactaaa gctcgtttag gaaattcttt aaaaaagttc atggcaacaa tgccacttga
    30961 tacaataagc gttattaaca tcacgaaaga ttgtaaagtt aatcgccaaa ctttctatta
    31021 tcattttcgt aatatttatg atttattaac atgggtattt ttaaatgagg aaattgaaaa
    31081 accatctaac aatgaattag aagaagcaat ctttattgtg ctgcgttatg ttaaacataa
    31141 cgaagcaatg attgaaaaca ctttagcgag cgctgctaaa gatttggtaa gagagtttct
    31201 ttttaattat ttatataatg tgtgtttaaa ttacattgat aaaaaagatc catttgaaaa
    31261 attaacatta aacgaacgaa aacttttagc aagagtatat ggcgcaggta tcgctagtag
    31321 cattatttcg tggattgaag gcgaatactt attccctgaa cacgaatttg tgatgaattt
    31381 agtaacattt tttaatgact tcccgcttga cgctataaat tataagggac acggctatgc
    31441 atagttttaa cttaaagatt caaactgaac tcatttttgg taacggccgg catactgaag
    31501 taggcagtat aattaagcgt tacggcaaaa agaatgtaat tatattatat ggtcaaaatc
    31561 acgcagtaag aagtgggctt ttagaagcag tgactaacgc acttgatgct gaaaatattg
    31621 attatgtact ttatggcggg attacgccta atccggaagt cttttatgtg cgtgaagcca
    31681 aagcgattgc aaaagaatat gcagtagact ttattttagc aattggtggt ggcagtgtaa
    31741 ttgatgtcgc aaagtcacta tcagtgaatt tttattatga aggcgatgca ctagatttta
    31801 gtaaacggat agttactcct aaaaaagcat taccgcttgg tgtaatctta acccatgctt
    31861 cagcgggtag tgaaatgtcg gcgagctgtg taattagtga tagcactaat aattttaaac
    31921 aagggtttag aaacgattta gtaagaccgc tttttgctat tactaatcca gaattaactt
    31981 atagtgtctc aatgtatcaa acagcagtgg ggattgttga tacatttatg cattcgcttg
    32041 aacgctattt taataaaagc gacaaaattg agttagccga ccgctttgca gaagcgatat
    32101 ttgttacttt acgggaatgc gcagagacac ttattgctga cccacttgat gaaacggcgc
    32161 gcgctaattt aatgttagcg agcactttta gtcacaatga tgtaacaagc atgggtaaaa
    32221 aagctaattt agttgtccat actttagaac atgcacttag tgcgctttat ccaagtattg
    32281 ctcatgggca cggtttagcg gtgctctatc ccgcatggct ctttgtttat tttgaagaat
    32341 taaaagagaa actcgatcgt tttgcgcgtg ttgtttttaa tcttcatcat gaagacattg
    32401 atacaaacgc aaaagaaggc attttagcac ttaaagaatt atttatgcgt ttaggattaa
    32461 caactacttt atctagttta ggtgttaagc gtgaagatat ccccacttta gcggatatcg
    32521 taacggaaaa cggcacaaaa gagttgtatc acgaccaaaa aaatctcaca agagatgata
    32581 taatcaaact ttatgaaagt tgtttttaaa actgttggag gacgaatatg aaaaaagaag
    32641 atttgcttag catattagtg tatcttgtca tgctaattat tgcgttattt attgggttaa
    32701 gaattattca acctgcgctt gacgcacttg atttagtgac tgatttacaa cgctacggat
    32761 ttgcgatatt aacaatcctt gttggtatta ttattaatgt tattttattt gagttaggcc
    32821 atgtctttgg tgcgcttctt ggtggctata gtgtgataag tgtaaacatc cttggattag
    32881 cttattacaa aacaaaaagc gggtggaagt ttagctttag acgttatgaa ggattaacag
    32941 gcgaaacaaa aattattgct aaaagcgata aaacaaaacc acggctttat ttgtttggtc
    33001 caacaataat ggtgcttttg gaatttgtgg ttgcaattgt tattttctta ttaacaaagt
    33061 ataatcaacc tattcatcac caagtgttaa tcgttgcggg gattggtgca atgcttctag
    33121 tctataacat catgcctttt aaacttgata acttcacaga cggatattac attgtgcttc
    33181 ttggcaaaaa agtaaatgtt gaagcgtata acgaattaat tagaattgaa tcgctcgtgt
    33241 ataacaatga aaaagttacg gacattaaag agtttgacga agtcacaaca atgacagcac
    33301 gtttaagttt atatcgctta tatcaattaa ttgatgaaaa agcgtgggac aaggcattat
    33361 cattaattga tgacttagaa aagaatgcga gtaaagttga acatgaattt attgcgagaa
    33421 ttagagcgca aaaactttat atttacttat taacaaaagc tagtgaagca gcaagtgctt
    33481 attggtttga tgaactaagc gccgcagatc gtaagtttat tagtaacgac ttaacaattg
    33541 aaacaatgcg tgtgtatctt ctctatagtg ggttagttac taagagccaa agtgaatgcg
    33601 cctttgtttt aagtcgtgct cctaaagcac tgcgcgctag aataaacgat ttccgtaaag
    33661 aagaagaaat agcgcttttt aatgaagcgt atgaaaaaat tgtggctttg cctggtaatg
    33721 aaaatctaaa acttccagta cttaaataaa ataaaaaaca ccagtaaatg tttacttaga
    33781 aactagaaac tggtgttttt cttttactac tttttaggtt ctttcttttt agttgctttt
    33841 gcttcttgtt ttttggccac taatgagtca cgaatttcac gaagtaataa tacttcttcg
    33901 cttggttctg gttctggctc tggctctggc tctggagcag ctgcagcttc ttcgtgaaga
    33961 agtttagcgc gtaaagcttc tgctttttca gtaagtctag ttacgacacg aacaatcaca
    34021 aataaaacaa gagcaattaa taagaagtta ataattgctt ggacaaataa accccatgaa
    34081 atagcaattt ctgcagtcac aacattgtca gcagcgtcac gaacttctgg cctaataacc
    34141 cattttaata gtgaaacctc attaccaagt aataacgaga taggcggcat aattacatga
    34201 tttactaaac tagtaataat tgcgttaaaa gcgccaccga tgataatacc aacagcaaga
    34261 tttaagacat taccgcgggc aatgaacgct ttaaattctt taaaaaactt tctcataatt
    34321 tatttccttt ctgctttcaa ttttagcata aagctaaaag taagtagtaa aacaaataag
    34381 accgtagtac tttaaatatg caaaataaaa taaaataaga tattattgat agtgaagtca
    34441 cttaaaagtg atgattaaag agttgtgagc aaatcacaaa aaggagaaaa tatgcctaaa
    34501 tttaaaatag tcgttgattc aacaattgat cttagtcctg aacaatataa aaagcttggg
    34561 gcgattgttc taccattaaa cgttcatatt ggtgaagaaa ctttccaaga cggcgtcgat
    34621 attaacgctg ataaacttta tgaaaaagta gcagaaacga aaacagcacc aaaaacgagc
    34681 gctgttgccc ctggagtatt taaagaagtg tttgaaaaat taaaagccga aggttataca
    34741 ggcgctttat ttgtggggat tggtgaatcc ctaagtgcaa cagcccaaag tgtgcgttta
    34801 gcagcagagg aagttaaaga ctttaaagtc cgagtcgtcg attcacactc actttcaaca
    34861 ggaagcgggt tattagcgct acgcgcttat gaccttctta acgaaggcaa aacacttgat
    34921 gaaaccgctg attacttaga aaaaatcgcg cctaaatcaa gagcgcaatt cattgttgat
    34981 aatttagaaa tgctcgctgc tggtggaaga gtaacaggca ttaagttatt ctttggtcgt
    35041 atactacgtg cccacccatt cttacaagtt aataatgaca agttagaagt agtagcgaca
    35101 ccaaaaggta aaagtgaaag agcattagac ctaatgctac aagtagcacg cgaagaaatt
    35161 agtgctggct tagaatcacc acgcgtcttt attacgcact cacacggcgg tgaacgcatt
    35221 gattatcttt acgaccgact aaaagaattt gtcgatccca aaaatatttt aattagtgaa
    35281 gcaggcgccg ttatttcaag tcattgtggc gcaggcacaa ttggtatttt atatattcgt
    35341 aaataacaat aattaattac taatgaaatg ccgcttaatc gcggcatttt ttagttgttt
    35401 tgcaactttg cacaattttc acaatacccg taaattgtta gatcgtgact tgtgggaaca
    35461 aaattatgtt tcttcatatt tttgttaatc atactatcta atttacaatc aatagggaat
    35521 cgcgcaagac aagtagtgca aatcatgtaa tgatgatgtt taccgtctat taagtaatag
    35581 taagctgttt tattaatagt gctcttgcct agaacatttt ctttttcaaa atattctaaa
    35641 gcacgataga ttgttgaaag aaaaatagat tcatttttta ataatcgttc aatgtcttcg
    35701 gcacaaagta atgaagattt tgttttaaat aaattaagca caagtttacg agcgggcgtt
    35761 aggcgcatct ttaagtctcc ttttagcaat gataatacga ataatatacg ctactatgag
    35821 aaatgtagta aataccataa tgattgcggg tcctgtcggc aaagtaaagt gataagaaac
    35881 agtaatccca ataaaagtag cgattagtga aataataacg ccgccccaca aagtcatatt
    35941 aaaactttta gataattgtc tagagattaa tgttggaaac aaaataaatg cagagattag
    36001 tagtgcccca atcgcgcgga caccgacaac aacaaaaata gaagtaagtg cggaaagggc
    36061 atatttaaga aacatagtgt taaccccagc aaaacgcgca tgagttacat caaaagtcgt
    36121 aattataagc ttacgataga atattaagat aaaacaaata acgagcaaag ctaaaactga
    36181 agcaccaata atttcaggaa cagtaactgt taataaacta ccaactaata gcgattccac
    36241 agagcgatta aaaccatcgc ttttactaat aataattaaa ccaagcgcta aagcaaagac
    36301 gctaacaaca ccaattgctg catcattatc tagattagcg cgctctgtta aaaaggtaat
    36361 ggcaattgag gctaaaagcg caaaaggtaa ggcaatataa aggggttcat tactaaatag
    36421 taatccaata acaacgccgg taaaggcaat atgcgccatg ccgtcggcaa ttaaagattg
    36481 tttagataaa acaagaaacg ggcttaaaag agcggccgct agggcaataa gcagcgctaa
    36541 caaaagagca taaaccataa aagtgtgttg taagaaacta aacatggata tttttctcct
    36601 tttcaaaata ctcttgtaag ccgccaaaga aacgtatttc ttgatcaata tataacacac
    36661 tagcgcagtc acaaagcgca ccatgaagtt catgtgtgac aaaaataatt gtttttcctt
    36721 cgtgatgtaa tgctgtaatt atgtcgttaa agaaactgcg aaaggacggg tcgagtgcag
    36781 aagtgggttc atctaaaata ataaaatcag gattactaat aagcgcgcga ataatataga
    36841 cgcgttgttg ttgaccgcca gaaagatggg ccattggttt tttaagcaag tgcgggattt
    36901 gcatcttttt aagccatgat tttattagtt ctcgatcacc tttactaatc agtagtgact
    36961 gtttagcaag gccgctataa attacttctt caaccgtgat tgggaaatta gggttaatat
    37021 ttaatttttg cggtaaataa cctaaacgat catcactatg ttcaatagtg ccatgcgtcg
    37081 gctctaacaa tttagcaagc aaccttataa aagttgtttt acctgatccg ttaggaccaa
    37141 tcacataaag gagcatccct ttattaattg aaaagttgat gtttttaagc gctatgacat
    37201 caccaaaatt tacacttaca tcagtgagtt taaccgccaa aggcttgttt gatgtttgcg
    37261 acattttgcg cgaagatgtc ggcatatgtt ataccctcct ttgcttgtgc tgcagtaata
    37321 ttgtgatacc catgaagttc taaaagagta atcgcataat tttcctttgc taattcattt
    37381 ttaattaaat tagcgacacg cggttccact aattcttcga tgaataagta atgcgaatca
    37441 aatgttttaa tttcatacat aaaatcaata atttcttgtg ataaaaaatc aacatctggt
    37501 ttataacttt cgctaattgt cttaatagtt agtttataag cagcagcaaa ataaccaagc
    37561 gcattatgcc ccgcaaagaa aagcggtggt ttttctaacg gtggaaacaa aaagtcacga
    37621 aaatcattac ttgtggctaa taatcgcgct gtgtaattat tatgattttc tgtaaaatag
    37681 gtctcatttg ccggcgaaat gtcaataagc acagttaaaa gatcattcat aacacggatg
    37741 tatatatgtg gatctgtcca gtaatgaagt gtgtgatcat ggtcgtgttc gtgatcgtga
    37801 tgttcttgtt tttttacttt gcaagcatat tcaacatcag aatgaatact catatcaaaa
    37861 ctatttttat tagtgacaag gtctttaacc catgtttcaa gcgcaggatg agtatagaca
    37921 aatagcttag cgttattaat atcttcaata tcacgtggtg taggttcaaa gtcatgaagt
    37981 tcactgcccc aagggacaat gtttttaact tttagtttgt caccaacaat gttagttaca
    38041 gcatcgtaac ttacaaacga agtaacaaca atatctggtt tttcgcgcgc cttacaaccc
    38101 gctaaaagta agaatggtaa tagtaataat aatttctttt tcataatcct tcaatttgcg
    38161 aatgattcgc aaaaagaatt ataacacaca gcttagcgtt atcgctaata aaaagcaaag
    38221 gcgccacaaa ggacgcctct taaatttaga taaagatact ttattactag ccagcaattt
    38281 ttaatgattt aacaagaata cttgaagtag tgtagctact aagttgtaat tcattttcat
    38341 tagcgaccgc cttaacatcg ttaaagagtt taactaagtt accagcaacg gtaattaagt
    38401 taacaggttc agcaacttta ccgtcacgaa tcataaaccc tgctgcttgt aaactaaagt
    38461 caccacttgt tgtgttgagt cccgagtgga gacccataag ttcagtgata aatacacctt
    38521 ctttaatatc tttaattaaa gtatcaaagt cgttacgccc aggttttaag gtgatattac
    38581 taataccgac actaattctt ccgcccgtgt tacggccgtg accggtaggt tcaacaccca
    38641 ttttagtagc ggtgcttaag ttatggaaga atgtttgtaa gataccattt ttaataattg
    38701 gtttatttgt tttagcaacg ccctcgtcat caaaataacg atagaagaga ttagctgtta
    38761 acggtgcatc aagaacagtt acttttgaac ttgctactct ttcatttagt ttgtcggcaa
    38821 ataaagaagt gttcttttca acacgatcag cacttgcgct tgttaagtaa gctgcaagta
    38881 aactagcggt catttttggg ttaaatacaa cgggataatc cttagtagca catggtttac
    38941 caccaagttt ttctataact ccatcagccg ccttcttcac aaatgtgtca gcgtcaaatt
    39001 tactataatc attatcaagg aaaacttcga catgcgattt aacatcatcg ccttctttag
    39061 caacaaccca tgttccataa tagaagtagt tttgtttatt tgtgagtttt aaaccatatg
    39121 aattaaataa tttataatca ctttcaatct cggaatattg aacagttgaa acagaagtaa
    39181 taagcgggtg cgcttttttt aacgcttttt caatggtaaa gagcgattca agttttgtgc
    39241 tttcactaat agtggcaagt tctttattaa agaatgattt tttacgatat ttaggactac
    39301 cttcaaaaat aatacttaag tcttctgttt ctgaaactcc ggcactcatc ttaatttggg
    39361 caactaagta ttgggcagtt gttttatcgt ctttttcact cattgcaaaa cccattttac
    39421 ctttatatat accgcgcgct agaacgttaa ttgtggacgc taaggtgtat ttttcaacac
    39481 tttcatcata cacactcatc tcaatacctt tatttttttg aataacgagt tcagcggctt
    39541 caaggccttt ttcttttgct aattcaaaga atttcttcac gttcattgta atgcccctcc
    39601 tctaccacca acggtaattt cactaactct aattgttggt tggccaacat caactggaac
    39661 actcccgcta tcggcaccgc acatgccttg agcgcgttca aggtcattag cgaccatatc
    39721 aattttcatt aatatatcag cgccattacc aacaagtgaa gcacctttaa ctaattcggc
    39781 aactttacca tcacgaataa tataagcttc atcacacgca aaattaaagt caccagttgc
    39841 tggattaact tgtccaccac caagtgattt tgcatataat ccaagtttta cactgctaat
    39901 gatttcttca ggtgtatatt taccagcggc aatgtatgta tttgacatcc ggcttgttgg
    39961 ttcaaaggta taattttgac gccggcctgt accattgctt tcaattccca tccgccgtcc
    40021 attaaaacga tcaacaagat aactttgtaa aatgccgttt ttaattaaaa catttttctt
    40081 tgtttttacg ccttcgtcat caatattgtt tgatccccag ccgtttacaa tcgtgccgtc
    40141 atcgactgcg gtaaccgctg ggtgagcaat ttgctttccg actttaccgg caaaaggtga
    40201 taatccctta ctaacagaac ttgcttcaag cgggtgacca catgcttcgt gaaaaataac
    40261 tccaccaaag ccgttaccaa tcacaaccgg cattttcccg cttggacatt ccgctgcggt
    40321 taatttacgg acagcaacat cagcaacatc acgcgcaatc ttttctaaat ctaaaccttc
    40381 aacatattca aagcctttgt gtgctcctgg tccattgtag ctatattcaa aggcgcctgg
    40441 tgccattgct attgcttgaa acaccaatct tacgcgctct tgcttatcag taaatgcatt
    40501 accttcacta ttaaaaatag tgatattacg aacatcagtt aataatacac tgatagcgcg
    40561 tacaatcttt tcgtcataat ctttcattac tttattagtg atttttaact tctcaacaat
    40621 ttcattaata tccactaatt caagactttt ttctaactcg tgatttttac gaacccttac
    40681 atttttaagt ttttcaacac ttaaaactct ttgaccatca aagccagcgc ttaacgcagt
    40741 ggcaagattt aataaacctt ttttgcttaa gtcacttgtg tgaccataaa cactttgtaa
    40801 tccttttaaa atacgaatgc ctgccccata agttaacgaa gtagcaggcg cttgcgtttt
    40861 accgttttct acagtgtagt taccgctgta agtttcttct aaaaatattt cagcgaaatc
    40921 tccaccggta ctaagcgcta catttaatac ttcgagggca agtttttttg taatcataat
    40981 gtacttcctc ctaattaaaa atgataacaa aaatatgaag gaaatatggg gaaaagcgct
    41041 tttttaggga attttagata atacaacaat ttaatattca aattgtaacg aataagggca
    41101 gctactaata aaaaagatta atatttcttt aaaaagcact aaaaaaatca cgctaaatta
    41161 tatgagcaac aaaattaaca attattggtt ttgttgtaac ttaacgaaag gaggagaaat
    41221 attaatgaag aataaaaaaa cataaatgaa ttaagttatg aagcaaaaaa agtttatgat
    41281 aaaagtcaat tttcaaaaaa agcatgtaaa agcaacattg ctttctatgt ttttgattgc
    41341 aataatcgtt caaattcaaa aaatctaact ttgagtaatt tcattattat aattaacgag
    41401 ttgattgaag cgcagcgata cagtgcatac catatagaag aaacatcaaa aaattactat
    41461 ttcataacat tcatttaaaa gaagaaaaaa gagttgtatt gatgttgatg ttgatgtgta
    41521 cccctttttt gttatcaaag atttggggta cacttcactt caaaaaaaga ttgtattgtt
    41581 tactatgata ttaaaatgca actatctgtt gcaagtaata attttactag ggtacttgtt
    41641 ttgggaactt tttattgatt tcataaaata tcataaaaga gattattaga actcaggaat
    41701 aatattttcg gttgtcaaat aactgtaata atgatattat tacttttgct tacttaggtt
    41761 aaatcgagtt aacctaggcg gaaggagata ttatgaaaca aggaattcac ccaaagacgc
    41821 aaaaaatcac agtgacttgt gttagctgtg gtactgaatt tgaagcatta tcaacaaaaa
    41881 aagaaatccg tgttgacact tgctcaaatt gccatccgtt ttatacaggg aaacaacgct
    41941 ttacccaagc tgatggtcgt gttgataaat ttaataaaaa atacggactt aagtaaattg
    42001 attactgaaa aactgttaaa tcaaatggca gttttttcaa atataagagg tacactatga
    42061 aaaaacgttt ttacataaca acccccattt attatccaag cgctaagtta catattggtc
    42121 acgcttactg tacaacttta agtgacattt tagcgcgcta taatcggatg attggtaaag
    42181 aagtttattt cttaactgga agtgatgaac acgggcaaaa aatagcggaa aacgcggcaa
    42241 aagcaaataa aacgccacaa aagttcgtgg atgatattgt aaaaacattt aaagacctct
    42301 gggaaaaatt agcaataaca aatgatgatt ttattcgcac gactgatcca aaacacattg
    42361 caactgtgca gaagattttc accaaacttt tagaacaagg tgatatatat cttgggagtt
    42421 acgaagggtg gtactgtact ccgtgtgaat cattttggac tacaactcag gttggtgaag
    42481 aaaaactttg tcccgattgt aatcggagcg tgcatttagc aagtgaagag tcatatttct
    42541 ttaaaaccga taaatatgtt gaaggattat taaagaaata tgaagaaacg ccaaacttca
    42601 ttacgcctga atcgcgtaaa aatgaaatgg ttaatacatt tattaaacca ggactaagcg
    42661 aacttgccgt ctcacgcacg agtattgagt ggggagtgcc tgttagagaa aatgagcgcc
    42721 acacaatgta tgtgtgggtt gacgctttaa ttaattatat ttcggcatta gggtatttaa
    42781 gtgaagatga tgaactcttc cataaatttt ggcaaagtga agatgttgaa atagtgcaaa
    42841 tcttaggtgc cgatatcacg cgcttccaca cgatttattg gccaatgatt cttaaagcac
    42901 tagggttacg tcaagcagac cgttattttg tccatgggct actgatgatg aaagacggaa
    42961 agatgtctaa atcaaaagga aatgttattg atccgttgcc gcttattgat aaatatggcg
    43021 tggacgctgt gcggtattac ttagcaagag aagttgtgtt tggtaatgac ggacaattta
    43081 cacctgaaca attcgtcgaa cgctttaatg ttgatttagt aaatgacttc ggcaatttat
    43141 taaatagaac agtatcgatg attgaaaagt attgtgatgg tgttattccg ctttatagag
    43201 gtcaagtaac tgaatttgac accgaaatcg aagacttagc gattaatgtc gttaaagaat
    43261 atcacgaaca ctttagtgat ttaaaagtga cagaagcgat taccactgct tttaaaatta
    43321 ttagtgcatc aaacaaatac attgataaca ctaaaccgtg ggaattaaac actcaaggta
    43381 aaaaagaaga attagattca gtaatgaacc acttagcaaa taaccttttt attggcgcaa
    43441 tattactgca acctgtacta gtaaacgcac caaaagaatt atttaagcaa cttggggtgc
    43501 cgcaagataa acaagatatt aatctattaa ataaatatgg tgttttaggt ggagaaaaag
    43561 ttaataaggg cgaaccatta ttcccacgcc taaaagttaa tgaagaagtc gaatacatta
    43621 aaaacttaat gaaataatga ttaaagaaaa tgacatttat gtcgtaactg ggggcgacta
    43681 cactagtgaa gggattggcc tagctaaagt cgataatttt cctattttca ttaataactt
    43741 aattattggt gaagaagcgg aaattaaaat aacgaaggta atgcgtaatt ttgcgactgg
    43801 taaaataatt cgttttacta aaatgtctag tgatcgtatt acaccagttg tcccggaaac
    43861 aatccatctt gcaggttgtc aatttaccca acttgattac gcaaaagaag tggaatataa
    43921 actttcaaaa gttgaacgag ccttacgcgt tatcggtggc attgatttta aaattgaaaa
    43981 gatccacact gctaagaacc catatttcta tcgtaataaa acagtattac cgtttggcaa
    44041 aagaaagagc ggcaaagttt ataccggttt atttagacat aacacgcacg aaataattgc
    44101 gatggagaaa acacatcttg atgacgaatt agcaagcata gttattgctg aagttcgtaa
    44161 attaattgag aaatataatt acgaagttta tgacgaaaaa agtcatactg ggcaagttag
    44221 aaaagtaatg gttcgtgtaa gtcattatct tcgtgaagta atggttgtac ttattacaaa
    44281 agatgagcaa attccaaatc tagacgcatt ttgccgtgat ttagtggcaa tttgcccaaa
    44341 tattacaacg attattcaaa atatcaatag cgaagatacc aatgttattt taggtaaaaa
    44401 ggaaaaagta ctatatggtc ctggttttat taatgaagag ctacacggac ttaaatttac
    44461 gctttctagt aaatcatttt tccaagttaa tacactccag gctgaggtgc tttacgaaag
    44521 agcactaact ctagcgggca taacaaaaaa tgatgtggtg ttagatgctt attctggcac
    44581 aggcacaatt gctattttag cagcaaaaaa agctaaacaa gttatcggtg ttgaaattgt
    44641 taaagacgct gttaaagacg cagaagtaaa cgctaaacaa aataatatta caaatgttaa
    44701 gttctttaaa gatgacgctg gtgctttttt aactagagaa ataaagaact ataacttcag
    44761 cgttgtcatc gttgatccac cacgtaaagg attgagtgac gaatttgtta acactttact
    44821 tagagcgcta ccgcctaaaa taatgtatgt atcatgtgac cccgcgactt tagcaagaga
    44881 cttaaaaaag ttaacaaaga agtataatgt tgaacatatt gaatgtgttg acatgtttcc
    44941 gcggaccgct cacgcggaga cgattgttag tttgatttta agaaaataat atactcatta
    45001 tttataaggt aattatagaa cgctcttcaa tagataatct tttttattaa tatagcaaac
    45061 acatatttta ttctataagg actattagtt tttaaaaata ttaaaaaaga tgtattttta
    45121 gaaaggaata caaaatgatt ataaagcaat tttaaaagaa aagtgtaagg aaaaattttg
    45181 actaatttat cctgctgttg aggcaaacaa acttgaggga aaaatttgag aaatatggaa
    45241 gtgtttaaat ggataataat taatatataa gaacaatcat gtttgcgtgc tacaatgtaa
    45301 aatagagagg tgggttaatt taaaataaac aaaaaattta gttattaata ataattgtgt
    45361 aaaaatttat gtgtccttaa aaacaaccaa aaataaaaaa gaaaaggtgc ttttaattgc
    45421 cggaaaattc cacattaata gatagaatat gaaaaaaaat aaaaaaataa gcaaaaaaac
    45481 tcgggagaat tggcgttatt tagtaacaca ttttacacca gagtacattg aagcaatgac
    45541 cgataaaaag gaatatgtag ttggatgggg atcagacaat aagtcgtttt gttatttggt
    45601 ggaagtgggt acaaaagagt taggtgagat tcgtggcgca aattcttcaa agtttggact
    45661 ttggtatgga actcatggag aagataaaga gattaaatat agagcgacaa aacaacattt
    45721 taattgtagt gttaataaag catttgaaga catcaaaatc gcattggcaa aattaattag
    45781 agaaacgaga gaacttacgg aatttaaaga tttagaatcg ttgttttctg gcatgtttaa
    45841 atataaaatt atgtacctat acaacccatc tattatgata ccttgttttt tacttgaaga
    45901 tttacaatac tttgaagagt atttaattgg taaaagtagt aaaacttttg aaaatgcgca
    45961 aaaagcgctg attaaataca aagataaaca tttttcaaaa aaagataatc atgacttcat
    46021 gatttttttg tactcaattc gtgaaaaata caatgtaatc caaacaaaag aactaaatga
    46081 agaagctgat aataaattaa ataaaaagtt atcaaaagaa aaagaatcta catatatcta
    46141 tattacgcac ccggttgata aagttagttt gaagaagggg acgggagggg gactatatta
    46201 tcctcgtgat gtaaagatgg ctgcaattgc tcttaaaaat gctaactata agtgtgaagc
    46261 gaattcaaat cataaatgtt ttttgagaaa aagcaacgga atgccgtata ctgaagtcca
    46321 tcatttaatt ccgctcgcat atcattataa atttgataaa tcaattgata ttccagaaaa
    46381 catagtttcg ctttgtagca attgtcataa cgaaattcat tatggtagag atgcaaataa
    46441 aatgattaaa aaactattta ataaaagaaa gaaaaaactt catgaagcag gagttgatgt
    46501 gtctttaaaa gaattatttg
//
LOCUS       DAOSTA010000005        35978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_1297_length_35978_cov_11.450519, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000005 DAOSTA010000000
VERSION     DAOSTA010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 35978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35978
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1297_length_35978_cov_11.450519"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..3644
                     /locus_tag="PLU08_01080"
     CDS             21..3644
                     /locus_tag="PLU08_01080"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015373724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOQ70337.1"
                     /translation="MKTINDKTKRLHNDRVDFSKISGNFEVPNLVGIQIDSFEWFKNE
                     GIAEVFNEIFPVESYSKDIEIGFEGFRFDTPKYTYLECKDRGFTYSAPLRVTLSLGIR
                     KTGEVVKSEIFMGDIPLMTHTGTFVINGAERVIVSQIVRSPGAYMSKELDTKSGKFIY
                     NADLNPTRGTWLNFENDAKGLLSVRIDKQRKMNATLLLKALGITDYHETIELFDVFDA
                     KNRAKNNKRKSEEEMTSLELTLRKDESFTTSLEALYEIFRRLKPGEPMSDEGVKNHLI
                     QKFFDPKRYDLGKAGRFKFAKKLGIYERLEGVVLAEDLVSVDGEILFEKEHQMKKEDV
                     DKLRALKFFEKGAHTYTIPFNPDLDDYNTVNIVKAYVNDEKTQAINIVGTDLNIDEKR
                     VTTPDIVAVFSYFLNLMHGIGRVDDIDHLSNRRVRPVGELIQNQFRVGLSKMVKAMKD
                     SSSIADTETITVQKLVNTRAVASAIRDFFATSQLSQFMDQVNPLAELTNKRRISALGP
                     GGLTRDRASFDARDVHYSHYGRICPVETPEGQNIGLINNLACYAKVNEYGFIETPYRK
                     VLKPYGQITNETIYLDADEEKEFVIAEANVEVDENGVIQTQEIIARYQGENVLVSPDK
                     VDYIDVSPKQIVSIAAASIPFLENDDAMRALMGANMQRQALPLMVPESPYVGTGLETK
                     IAHDSGSAITAHKDAVVTYVDGANISVKFDDDTTFTYKLQKFARSNQGTCFNQVPIVK
                     VGDKVKKGQVLADGPSMVNGELSLGRNVTIAFMTWDGYNFEDAVIMSDRLVKEDVYTS
                     IHIEEYKIEARKIKTVSERITRDIPNVSEAAKAFLDENGIIIPGAEVKEGDILVGRVT
                     PKGQREQSPEDRLLQTIFNEKVREVKDTSLRVPHGGGGTVIDVKVFTREAGDELGPDV
                     EISVSVFIGQKRKISEGDKMSGRHGNKGVISRILPEADMPFMEDGTPIDIMLNPLGVP
                     SRMNIGQVLEIHLGMACKKLGVKVASPVFDGMTNEEIFELMEEAKMHKDGKEILYDGR
                     TGDRFDERISVGVMYLIKLVHMVDDKLHARATGPYSLVTQQPLGGKAQNGGQRFGEME
                     VWALEAYGAANILQEILTIKSDDRVGRKRTYEAIVKGYDLPTPGIPEAFKVLVRELQG
                     LAINVELRMKDGEILDLDKLAKEQDADIRRSRRSTAVREEQEVFDPEETAVVYKNEER
                     GDE"
     gene            3647..7513
                     /gene="rpoC"
                     /locus_tag="PLU08_01085"
     CDS             3647..7513
                     /gene="rpoC"
                     /locus_tag="PLU08_01085"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOQ70338.1"
                     /translation="MARKQPFNINDLASVQIGLASPDMIREWSNGEILKPETVNYRSQ
                     KPELDGLFCERIFGPSKDYECHCGKYRRIRHRGTVCEKCGVEVISKYVRRERMGHIEL
                     ASPCTHIWYLKGTPARIATLLDIPAKQLEEIVYFISHIVTDPGTSSVLSYKQFIDETT
                     GRQVFAEAIKEIQENIEEGSFEHAKGSELLERLEVTQTFDFFATSRYISKHTGAKFGE
                     GAEVIKQLLKEVDLQKEFDEVNAALKTESKTKEKKLLRRLEVINAFLKSGNRPEWMVL
                     DVIPVIPPDLRPMLQLDGGRYATSDINDLYRRVISRNNRYKALVQMNAPKVILMNGQR
                     MLQEAVDSLIDNGRRGKPYTGANKRPLKSLSSSLKGKQGRFRQNLLGKRVDYSGRSVI
                     AVGPELKMYECGIPREMAIQLFRPFIAAELIKRDSRATNHTQADKIIDRHDEIVFDII
                     EEIIADHPVLLNRAPTLHRLGIQAFQPVLVSGRAIRLHPLVCAGFNADFDGDQMAVHV
                     PLSEAAQEEALNLMLASKNILGPKDGKPIATPSQDMVIGNYFLTVEESREDFVRRAEE
                     ARALGDEFGAQKFMQYAESEGKVFGSIDELMVAYQTNSVHLHNRIALPGRAFNKTLFT
                     PEMNKSYLVTSVGKVIFNTIFPNDFPYLNEPNKGSLQMDKIEYFVPAGTNIREHIASL
                     PLRKPINQEFLGLIINEVFTRYGTVKTSAILDKIKDQGFHYSTKAGLTVSLHDITPIE
                     GKEELVEAGEVEVRKINKAAARGFYSEKERYNAVINVWKGVKDDVEAKLKEQFPLDKR
                     NPLYIMFDSGARGKTTNFLQLKGLRGLMASPRGGEIEIPIKSNFREGISVSEFFIASH
                     GSRKGGADTAIKTADSGYLTRRLVDVAQNVIVTEEDCGSDHGFVVTEIRIRENDTPAV
                     PLSDRIAGRYTVHDIIHPTTGEVIIEANTLINKDAALYIEKAGVKEVEIRSLFGCEAP
                     SGICAHCYGTNLATGERVKVGEAVGIMAAQSIGEPGTQLTMRTFHSGGVAQVEDVTQG
                     LPRVQELFEARNPKGKAIITEIAGEVVSITQEEDRRYTVTVKNDLEEKSYLTEYGQQL
                     RVKEGDTLKQGDRITEGAVHPRELLAVSTITAVQNYLLNEVQKVYRISASVRISDKHL
                     EIIINQMLNRVYIIDGGDTSLLPGQRITTHQFTAANKEALINGLHPAVATPLILGIKA
                     AALASDSFLSAASFQETTRILTDATVKGKLDPLHGLKENVITGRLIPAGRGILTKEEQ
                     EALLEDFSVADTLDKIKASYDNSSNS"
     gene            7654..9249
                     /locus_tag="PLU08_01090"
     CDS             7654..9249
                     /locus_tag="PLU08_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70339.1"
                     /translation="MKKRLLLLPLLAMLLGGCNGGSSATPSISTPESIPSSEEPSVES
                     SAESVSVSIDTTSYEIATTTNYSNRELDHSIDMTALDSLGTQKMLVVPVVFSDTAHRA
                     TEEVHTMINKAFFGASEETGWESVASYYHKTSFGKLTLTGEVLPYFYSQWSTHAFGAL
                     EVSPTQEYGEGYYWDQTHHLIEEIYNTLDAELFLEYDQDGDGHVDAFWMVYIAEIKAL
                     GGEDDPFWAYKFYWNRYANTEKPTPNTYAWASYKFADEGVGYNFNIPDAHTYIHETGH
                     MFGLPDYYDYDSKTAPAGALDMMDHNVADHNMYSKYRLNWSKPFVVTGNADIHLRPAE
                     STGDFILIKDGWNGHVYDEYILIEYYTPTGLNEKDSQGRGYETKSSNVSTKSFDINGV
                     RMWHVDSRLMTYVFDPESLELTNAFWTDEIVVGDPFSYTIIGPSNTTSRSSTPEGDSK
                     VNKLLHLMDATGTTSRVGNWLRTPTTAGSTALFQTGDVVAADGWKKYIQTSATFNDGT
                     AVGYSVEIGEMDDNGVQIIIRKA"
     gene            9313..10833
                     /locus_tag="PLU08_01095"
     CDS             9313..10833
                     /locus_tag="PLU08_01095"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HOQ70340.1"
                     /translation="MKCKVKRNTKKVTLLLSLLLLSSCNLEVTFFPYTHHISDTDTYT
                     RIETSDYYKVGAFARSYQDVLKSNLNGNSRVVPLKSSGEQKILVLPVNFPDYDLNELD
                     GNNGKTAHIHIQNAFFGENHTTRFYSVAGYYEASSYGKLKLSGDVAPWYTLPAEYSVS
                     AIKSRVRSNSDKLNETAEILNLALKEYAKSNDLSAFDLDENGVLDSAYVIYSYPFEPK
                     DVNNIFWAFAANMNKKDPELTHQANAYSWSSYYYLDVARNKKPDPHTYIHEVGHLFGL
                     PDYYNTNYDDPYSPLGGFDMMDYTLGDHTGLSKMLLDWARPYALKRSTTIKLRPFSET
                     GDLLVLKNNWNEKANDEYILLEYYTPTVLNELDSTLNASFKLPKTNGVKVYHVDARTV
                     YEIKNGSIVKYPYVNDYEGEFNGTELLAHSNSTGSYNNAPIKTNKLYKLLEPKESTNI
                     SESFNQANQHSLFQKGDEFGYEYFKNFAFNDKTPLNFRFKIVQLTNAYATIEIEVY"
     gene            10835..12457
                     /locus_tag="PLU08_01100"
     CDS             10835..12457
                     /locus_tag="PLU08_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70341.1"
                     /translation="MRKSTFIRRALILVVPALLLTSCVTFGDILKSSEFGATSSSANE
                     VTSEVTGSENPYPLNGTYGVNDYEHPITANPDTLNNYYKVEPLNNNSRLINNYAISNT
                     MGLAHLDSVSNQKMTVVPVKFTDTPASYGGEATIQGAINRAFFGESSDTGWESVASYY
                     YKSSYGRLSLTGEVMPLFDMGYSTTELLAKTVDGYQYWDKSHYVLEQVYNTYLSSTLK
                     EYDQNNDGFVDSVFFVYLAPVDYESDDTLFWAFQYFWNRFADKNKPVFNAYGWASYEF
                     MVEAPGYTYLKPAAHTYIHESGHILSFDDYYDYDGTIAPAGAIDMMDHNIIDHNMFSK
                     FSANWSTPYVPVGNSDIRLRPAESSGDFIIINDAWNGHAYDEYILIEYYTPTGLNARD
                     SLSVSGYNGVKGYTIPGVRIWHVDARLVILSNNGNTKKWSDTIPPHLDGSGGSWMDFA
                     TSNTPSYNYAHNSLHKLHLLDGRGRAQTWANNLVKADDSALFQTGTVIASNNWQKYLL
                     KRATFNDGSVIGYSIEIGEMNSEGVTIKIRKA"
     gene            12512..13465
                     /locus_tag="PLU08_01105"
     CDS             12512..13465
                     /locus_tag="PLU08_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ70342.1"
                     /translation="MATLKDIAKATGFSVITVSRVINSPHLVKEKTRKIIEAEIARQN
                     FKPNVLARALVQNRTNIIYVYIPADIDAINPFFLHVVSGIGEVLGEKGFSLLLKRSWY
                     EGEDCDGCIFMGLSAEDEAKVFELSKTKPVCVFGYVEGVSSVDVDNYQGLYLMTSHVL
                     SKGYRTLTYLGIDDYRRFILQRKKGFSDAVMSQKDKITVKYLAAKNNENDAYKVVGQS
                     LKQGDTPEVIVCATDDMAIGAIRAIKEKGLEVPRDIAVTGFDGLGTQEVAHPRLTTIH
                     QPIYEAGKALAHRIIALIENKNELPVNHAFIETTIIHRESI"
     gene            13552..14070
                     /locus_tag="PLU08_01110"
     CDS             13552..14070
                     /locus_tag="PLU08_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ70343.1"
                     /translation="MKPKKITTILFSLFLALSLSVVTGCKDGSENGGSDDPINFWAYQ
                     PSKQTDQTAYKNLIADFTAETGIKVKLLMVVKDSYNKSLNAAISSRSKPDLAYFDQPR
                     ISDYAHDDTIAKLDEYIANSAVIDNTKFFASGLATTVYKGSQYGVPLNMTTSVLFYNK
                     DIVTTPPTSWQE"
     gene            14242..14919
                     /locus_tag="PLU08_01115"
     CDS             14242..14919
                     /locus_tag="PLU08_01115"
                     /inference="COORDINATES: protein motif:HMM:NF028048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starch-binding protein"
                     /protein_id="HOQ70344.1"
                     /translation="MFYDPNKVLSSGDVYLYTWDGDGRKITGNWPGTAMFNHGFGYYS
                     WFFHAAYLTENVIFHDNAGNQTGDIVWEIGKNVYHQETGTWTFVSEVHHKATVYASKI
                     LEKTATCDATGAANNLNAPEWNETLANQYNDSLDPTTKAYLKTAVADSNGDLLAQAMA
                     RYDYIVKKYGTAMFSNFLDRTIPASGSPLVSNEGTNNALVMGAFIVMALVALAGFKLI
                     SKKHRYH"
     gene            15041..15277
                     /locus_tag="PLU08_01120"
     CDS             15041..15277
                     /locus_tag="PLU08_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOQ70345.1"
                     /translation="MIIKTEQIITMTDANQNFSKASKTAEKYGSAVIFKNNKPKYILA
                     DIEQYIELTEDEKIEIVAKRILSKHINAFKNLAK"
     gene            15348..15467
                     /locus_tag="PLU08_01125"
                     /pseudo
     CDS             15348..15467
                     /locus_tag="PLU08_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            15480..15653
                     /locus_tag="PLU08_01130"
     CDS             15480..15653
                     /locus_tag="PLU08_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70346.1"
                     /translation="MDGNKRIGLYVMLSFLQINGIIISFTDDEIEQIAFGIASGAINY
                     VDLKRILEQKIDN"
     gene            15909..16331
                     /gene="rpsL"
                     /locus_tag="PLU08_01135"
     CDS             15909..16331
                     /gene="rpsL"
                     /locus_tag="PLU08_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018395150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOQ70347.1"
                     /translation="MPTINQLVRHGRQKAVYKSKSPALTKRWNSLKRREVNQYSSHKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNGYEVTAYIGGEGHNLQEHSTVLIRGGRVKD
                     LPGVRYHIVRGTLDTAGVEKRRQGRSQYGTKRPHTAEH"
     gene            16379..16849
                     /gene="rpsG"
                     /locus_tag="PLU08_01140"
     CDS             16379..16849
                     /gene="rpsG"
                     /locus_tag="PLU08_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOQ70348.1"
                     /translation="MPRKGSVAKRDVLPDPIYHSKLVTRLINKLMYDGKRGTAQTILY
                     NAFERIEAKTKQPAMDVFATAISNIMPEVELKVRRVGGQNYQVPTDVSPERKTTLGLR
                     WLVNYSRLRNERTMEERLAAEIIDAANGTGASVKRRDDTHKMAEANKAYAHYRY"
     gene            16858..18939
                     /gene="fusA"
                     /locus_tag="PLU08_01145"
     CDS             16858..18939
                     /gene="fusA"
                     /locus_tag="PLU08_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004028937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOQ70349.1"
                     /translation="MADLTKFDLQHTRNIGIMAHIDAGKTTTTERILYYTGRLHKVGE
                     THEGASAMDWMAQEKERGITITSAATTAFWKENRINIIDTPGHVDFTVEVERSLRVLD
                     GAVTVLDGKSGVEPQTETVWRQGTKYNVPRIVFVNKMDATGADFNMAVSTLHSRLAAN
                     AEPVQIPIGVESNFRGIIDIIEEKTYIYDDNIANVSIEEVPEEYLEEMHIAKQNLIEK
                     LAAFDDDFMIQVLEGETPSVSEIKRVIRKAVLTGEFFPVFCGSAYKNKGIQPLLDGVV
                     DYLPSPLDVPPAKAELLDGTEIITPSDADAPLAALAFKIATDPFVGRLAYVRVYSGTI
                     KAGSYVYNSIKGVRERISRLVLMHSNHREEVSELKAGDIGAVVGFKNTTTGDTICDEK
                     NKLILERMEFPEPVIEQAIEPKTRADQDKMTNGLAKLMEEDPTFKVTTNPETGQTIIA
                     GVGELHLDVLVDRLRREFGVNVNVGAPMVGYRETISQEAECEGKYIKQSGGRGQYGHV
                     WIRFEPNPGKGFEFVDAIVGGVVPREYIKPTEEGLKDALVRGLVAGYQVIDIKATLYD
                     GSYHDVDSSEAAYKIAASMALKEAGRLAEPVLLEPIMKVEITVPEQYYGDVLSDLARR
                     RGSMKDESQRGNAKVIDAEVPLSEMFGYVTDLRSFTQGRGTYTMTFDHYGECPRYIAD
                     EIVKKSGTSAR"
     gene            19048..20232
                     /gene="tuf"
                     /locus_tag="PLU08_01150"
     CDS             19048..20232
                     /gene="tuf"
                     /locus_tag="PLU08_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009007562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOQ70350.1"
                     /translation="MAKERFDRSKVHVNVGTIGHVDHGKTTLTAAITKVLASQGGAKL
                     MAYDQIDRAPEEKERGITINTSHIEYQTATRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGVMPQTREHILLARQVGVPYVVVFLNKVDLVDDPELVDIVEMEVRELLNE
                     YGFPGDDTPIVRGSARDALDGKADAEKAIIELMEAVDSYIPDPERLTDRPFLLPIEDV
                     MTISGRGTVVTGRVERGMVKLNEPVEIVGIRPTKSTVVTGLEMFRKLLDSAQAGDNVG
                     VLLRGVARDEVERGQVLAKPGSVKPHTKFTSTVYVLTKEEGGRHTAFLSNYRPQFYFR
                     TTDITGVVTLPADVQMVMPGDTVTLTVELIHPVAIENGTKFSIREGGRTVGAGTVTEI
                     LK"
     gene            20344..21456
                     /locus_tag="PLU08_01155"
     CDS             20344..21456
                     /locus_tag="PLU08_01155"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOQ70351.1"
                     /translation="MKKEKKITQPNLDTVPGGALKRTGAVLIDLVIMYLLMNVLWFAI
                     HPIYNKVYDIDNVVTAHERDKKRSNLVVLNEPILDDESNIDLVKVESNQLPETKRGEG
                     VFKFYTVFLRSEQEAKLAAGEIDNTPENNAMFTFDWYLENVIKPDTEESIYLRIPTDE
                     HLGAEGAVSSEETAEPEYDTFRPAGLDFKADLTAEDINKFNNRIYNQAVNAFNLRPNM
                     TIINKVNIQENIILMVLGSTVVFLVFPLFFKHGQTLGKKALKLGVTTRHGYTVTVPWL
                     LLRYFAFLFINLLSNLLIPIILPFISLTIMTFSKERRSAHDFIANTKVIDLQNSTVYK
                     SEAEFLEAQKRYNPRLEAEDFNEEVFSERFDEESGA"
     gene            21456..22460
                     /locus_tag="PLU08_01160"
     CDS             21456..22460
                     /locus_tag="PLU08_01160"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOQ70352.1"
                     /translation="MNHYVVKQTAGKRFLAGFLDTIFVIILALFLYIPARTIVDNNGL
                     IEVFVKITQIQLKSGLYDVEGIDNAVPYIINDVEKFPKALYTFYVDENLETGEIERGY
                     ADILVEGHEYNTAEDYYVVILGKGREDSLFTFDAINSEAPWEVPFKEGKQEAVKQFYT
                     DEIEKAHQHLDYHEVVVPLIIKAERLIFYAMAGSYLTSALILIVIIPNFRKDKVTFGK
                     VITGTIVLNRLGYKMSVLQANMRNFAIFIFSFVFFFVPFHLISFAMTLFSKKQLSMYD
                     QLVATLVADKKATLVFKDVNEETKYRKELAQRLIAVDKRKAESREERLRDKEKLNKID
                     "
     gene            22554..23333
                     /locus_tag="PLU08_01165"
                     /pseudo
     CDS             22554..23333
                     /locus_tag="PLU08_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            24007..25110
                     /locus_tag="PLU08_01170"
     CDS             24007..25110
                     /locus_tag="PLU08_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ70353.1"
                     /translation="MSTKAGVLHLEKGPMQTKKTIAMPIWLKAIIALLPALFFLLVFT
                     IFPIFNTAFIAFVKDFYFQSGTGSFALASYFRQLDMHNNPDWWREYFAIIEPDVPFLQ
                     PVKPIIGFANFEYVLKDPLFSNALINTAILTIISVPLTIMISLLLAVSLNSIKPLRAT
                     YQTIFFLPYVTNGIALGMVFNTIFSHEVGGIMNQFIQFFGGKPVSWIRPLWSPEGTIP
                     LSKFSLGFAITVQSIWSGLAFKILVFMAGLASIDKQYYDAARIDGSSRSTIFRRITVP
                     LLSPQILYITITSFIGAFKAYQSVISIVGGGPIHFGGENRTMWITVVGYIYMNRNVEK
                     NIPLAAAGSVVLLIVILFITLIQMQVSKKRVHY"
     gene            25126..26184
                     /locus_tag="PLU08_01175"
     CDS             25126..26184
                     /locus_tag="PLU08_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ70354.1"
                     /translation="MTDKDKKVTEAVELTAEEEVKLSHIDDERFQPSYEIAKEYKTRA
                     IVVKIITYTFLTLFAFFMLLPFWFMISVSVKTPLEYQREVSTGLRLFTANPSFVNFRI
                     VLGMDLKDPLKEMNEALLHIVETSPERSFIPYFLNTIIVAVVSTVFTIVTTVLASFAF
                     ARLEFKGKNFLFAILLATMMIPGEMMLITNIQTAKWLGWENTYRALIFVHGVSVFYIF
                     YLRQTFQQIPNELFLAAKVDGYGEFSYLWRVMIPIGMPTIVTIIILNVMGSWNAFIWP
                     TLIAQGYNPTIQKLFKYNHTMRLVSNGLMSTFTGQFASYDTVKIAASMIVTAPLLLVF
                     VFFRKYIMSGVSRSGIKG"
     gene            26229..27824
                     /locus_tag="PLU08_01180"
     CDS             26229..27824
                     /locus_tag="PLU08_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ70355.1"
                     /translation="MKFRKLIFTAVSVLALTTLAGCLRPPMPPTSETPTTSSGGGGPT
                     EKIEVKFWTGFGASVTGALNPLFARFEQENPDIKVLYESKSGYPNLQQAINGSISTNT
                     YPHIANGYPDHLVGYINANILVNLGNPTNYINHPDYGVDINEYNSDYMAEINGLSDAA
                     TFGLPFNKSTEVMIANQSFFDAAKKRDPSIFIPKTWQDLAEVGPKLKAVAKDNGWFGK
                     LVLKKDGTAVAKPAEFDAALKEQVAVDMTLVNENQFIPFSWDSASNFFITIIRQWGAM
                     YTEKGNTIQNGYIRFQEEPHRTKTLAALSYFKELYEDGLIGLPDNFGEPLYSSTPFKE
                     GKLVLTISSSAGVGENLPGGTTEYPFELSINPIPYNGDLAAQKSVIAQGTNLALFTVG
                     QGRDPKAQAERLAAWRLLRFLTYEANYEFGKATSYFPVLDASKIDRDSQRYQDYLMYS
                     EFLNQTDGTPREKVIRSTAKLQANVYMDPEAGWNKFVDPGFLGSSEIRNTIESVMAEL
                     FKGKTPEQVLNDTVTKLARYVQK"
     gene            27839..29140
                     /locus_tag="PLU08_01185"
     CDS             27839..29140
                     /locus_tag="PLU08_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70356.1"
                     /translation="MKKTFVKIGLLLAVFGLLVGCDNSSSEVVPPSSASEEVVSSSEE
                     EVKLTEQEYQIAVNYIARSVPLVLERGVKVPTNVERAEAENPLPTPELKYGDNLLLIT
                     AGELKYEDEINGVYLYPEYTVTYSFLEQEPYAGFTFEYDDIGRYFAVPEYPRYEAEYD
                     NGGNPIHTVPPAKAARLYVTIHIEERFTTFNMDMILEPQAIIHDYNLADVRSVPTGSV
                     VRVYGYFNGYFSDWNNGVLCDGDVALGLFKLQEFNHMELGKMYAVVGQFTVYNGLPQL
                     QWIKTVSEVEPADHPTVKEPKAVVVSADDFADQLDIGNELSGPLMHKVGTTVVLNFPL
                     RYKAMLDRDGNAVEPGALELGKHADVVLTGKDSDNFEFEVKLSLNYHMGTDNQAAFRD
                     FFVTNADADIYFTGPLGAYNAINLAPFAFEGTLSLTPPVVS"
     gene            29229..29304
                     /locus_tag="PLU08_01190"
     tRNA            29229..29304
                     /locus_tag="PLU08_01190"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29262..29264,aa:Lys,seq:ctt)
     gene            29412..30821
                     /gene="trpB"
                     /locus_tag="PLU08_01195"
     CDS             29412..30821
                     /gene="trpB"
                     /locus_tag="PLU08_01195"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007156746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOQ70357.1"
                     /translation="MKYLTVKELSEKWKMSERRIRALIKEKRIEGVKYENKYLIPENA
                     KKPLDRRIKGQRALEDASLNRQDFDFKNYRKHYPTSDGYFGRFGGSFLPKHLQKAIDE
                     VYDGYLTIAKSAKFIAELREIRKNYQGRPTPVYHCLNLSNHLGKVQIYLKREDLNHGG
                     SHKLNNAMGQALLAKYLGKKKLIAETGAGSHGAAVAMAAAYFDLECDIYMGAVDTQKQ
                     RPNVDRIKVLGANVIAVKEGTQTLKEAVDAAFNAYAKEYKNAFYVLGSAVGPHPYPLM
                     VRDFQEIIGREAKEQFVAMTGELPDVVAACVGGGSNAIGMFQAFLEEKVNIVGVEALG
                     QGPTIGQNSAAVSLGKEMILHGFNSLVLQNEDGSVGDAYTIASGLDYPGVGPEHAFLH
                     EIGRVKYEAITDEEAAEAFFLLSRLEGIIPAIESSHAVAYAIKYARSVSAGSILVCLS
                     GRGDKDMEFMTREYPNMYK"
     gene            complement(30897..31346)
                     /locus_tag="PLU08_01200"
     CDS             complement(30897..31346)
                     /locus_tag="PLU08_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70358.1"
                     /translation="MILYIPVDFDRMVGITEKLKTPEHSKVTLTGKHNGVDFEQKYTL
                     TDGVWVGDEEYEDPHGYTIKKSVSDHVMTIKSFTVEQQQKYKYFTNSRKFKIEFRDYT
                     SDKDYSYGTFIYNRKGTLMEIHFDSRFVNEEYSINVSDNLLIYYDFS"
     gene            31398..31712
                     /locus_tag="PLU08_01205"
     CDS             31398..31712
                     /locus_tag="PLU08_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ70359.1"
                     /translation="MIVYMNEHFRDPNLTIAKIAESSGYSLSHFSKAFKLYMGHSPIS
                     HLNRLRLSEARYLLHTTHLPFKEIALQCGFKNISTFTESFKQSIGILPKDYRKKYQTP
                     NS"
     gene            31879..35406
                     /gene="nifJ"
                     /locus_tag="PLU08_01210"
     CDS             31879..35406
                     /gene="nifJ"
                     /locus_tag="PLU08_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOQ70360.1"
                     /translation="MKKKFVSLDGNAAAAEVSYLYTDIAAIYPITPSSPMAELVDLFA
                     SQGKLNYFGSKVKVTELQSEAGAAGAVHGALQAGTLATTYTASQGLLLMVPNIYKWVG
                     ELLPAVLHVSARSIAARSLSIFGDHQDIYAVRQTGVTMIASHSVQEVADLAHAAHLTA
                     IGSSAPVLHFFDGFRTSHEIQKVEVIDENEWKKLLPEDKVAEFRARALNPHANPVTRG
                     GAENDDIYFQGKEAQNTHYARVLDVACDYFAKVSKVTGRHYAPFVYLGAKDATKVIVA
                     MGSVTETIREVVADLNKKGEKVGLVKVHLYRPFSAKHLVDVLPETVKKIAVLDRTKEV
                     GALGEPLYLDVLTALKQQGRKVDVIVGGRYGISSRDTQPKHIKGVFDFLDNKPWHSFT
                     VGINDDVTKLSVPFDHDYHIDNGHTECLFYGLGSDGTVSANKSSVAIIGEHTDKYAQA
                     YFAYDSRKAGGPTRSHLRFGTNTIHSTYYVENASFISASQDSYLYKFDMIRYLKAGGT
                     FLLNTSLKGEELINALPNRVKYGLAMKNAKMYVIDANKIASEIGMGRHTNTILQSSFF
                     YLNQDIMPYEKALRLMKKYAEAAYGRRGKRIVELNYAAIDAGSHGIIEVKVDPEWKKL
                     KPLRTVELMGDKYFDEYVNPIDNLEGYDLPVSTFTKWDLLDGTMTNDITFKQKRSIAT
                     MVPRWVKENCIQCNTCSLVCPHATIRPFLLDEKEVAGLPENVREETVAAMGGPSVAGL
                     RFRIQVSPMNCVGCALCVVTCPGRGGNKALEMVEARSQFDIEPAADYLYKQVTYKTDK
                     FPLNSPKGVGFLMPYMEISGACAGCGETPYYRLITQLFGNDMLVANATGCSSIYCGSV
                     PGTPFTTNKDGEGVAWANSLFEDNAEYGFGMRMANDYKLKLIADIFTAALPKVEPELK
                     ELLEKYLANPRDRKEVRSYLPRLIELLEKSKDKGVKEALKYKDDILDKSVWIIGGDGW
                     AYDIGYGGLDHVLASGANVNVLVLDTEVYSNTGGQMSKATQTGAIAKFAASGKTGAKK
                     NLAMMAMAYGDVYVAQVALGANPVATIRALTEAENYDGPSIVIAYSPCTEHGIKGGLS
                     NHIAVEKKAVASGYWPIFRYDPRLEAAGKSPLTWDMKEPDYTQFREFVMGETRFSQLP
                     TVNPENAEFLLSKSEEEAKKRYFRLKKFE"
     gene            complement(35618..>35978)
                     /locus_tag="PLU08_01215"
     CDS             complement(35618..>35978)
                     /locus_tag="PLU08_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70361.1"
                     /translation="GGYIDYARQLGKVVDKSICEPDQKWHFFDSWLYRTLEEGGITWT
                     DSAKNRVYTKLLCPELLLWIYEACAAPEAKILAAYDAAVAGKEAGTHISTIAKNMRAC
                     VPWEDLEINIQYFLDNK"
BASE COUNT        11524 a         6707 c         7514 g        10233 t
ORIGIN      
        1 caagtaaagc gaggccattt atgaaaacaa ttaacgataa aacaaaaaga ctccacaatg
       61 accgagttga ctttagcaag atcagcggaa acttcgaagt tcctaatctt gtaggaattc
      121 aaatcgactc attcgagtgg ttcaaaaatg aaggtatcgc ggaagtattt aatgaaatct
      181 tccctgttga atcttatagc aaagacattg aaattggctt tgaaggattc cgctttgata
      241 ctccaaaata tacgtattta gaatgtaaag atcgtggctt tacttatagc gcaccattac
      301 gcgttacatt atcgcttggg attagaaaaa cgggtgaagt cgttaaaagt gaaatcttta
      361 tgggtgatat ccctttaatg acacataccg gtacattcgt tattaacggt gctgaacgag
      421 taatcgtctc gcaaattgtt cgttcaccag gcgcctatat gtccaaagag ttagacacta
      481 aatcaggtaa gtttatttat aacgctgatt taaatccaac gcgcgggaca tggttaaact
      541 ttgaaaatga cgctaaaggt ttattatcag tccggattga taaacaacgg aaaatgaatg
      601 caacgttgct tttaaaggca cttggcatta ccgattatca cgagacaatt gaactctttg
      661 atgtgtttga cgcgaaaaac cgcgccaaaa ataataaacg taaatcggaa gaagagatga
      721 cttcgcttga attaactttg cgtaaagacg aaagttttac aacttcactt gaagcacttt
      781 acgaaatttt ccggcgctta aaaccaggcg aaccaatgag tgatgaaggg gttaaaaatc
      841 accttattca aaaattcttt gatccaaagc gttatgactt aggcaaagcg ggtcgcttta
      901 aatttgcgaa aaaacttggt atctacgaac gtttagaagg cgttgtcctt gctgaagatt
      961 tagtaagtgt tgacggcgaa attcttttcg aaaaagaaca ccaaatgaaa aaagaagatg
     1021 tcgataagct ccgtgccctt aaattcttcg aaaaaggtgc ccatacttat acgattcctt
     1081 ttaaccctga tttagatgat tataatactg taaatattgt taaggcttat gttaatgatg
     1141 aaaaaacaca agcaattaat attgttggca ctgatttaaa tattgacgaa aaacgcgtca
     1201 cgactcctga tattgtcgct gttttctcct atttcttaaa tctaatgcac ggtattggtc
     1261 gcgttgacga tattgaccac cttagcaata gacgtgttcg tccagttggc gaattaattc
     1321 aaaatcaatt ccgtgttgga ctttcaaaaa tggttaaagc aatgaaagat tcttcatcaa
     1381 ttgctgatac agaaactatt actgtccaaa aacttgtcaa tactcgagca gtagcgagtg
     1441 cgattcgtga tttcttcgcg acaagtcagc tttcgcaatt tatggaccaa gttaacccac
     1501 ttgcggaact tacaaacaaa cggcgtatta gtgccttagg tcctggcgga cttacccgtg
     1561 accgcgcaag ctttgatgcg cgtgacgttc actattcgca ctatggtcgt atttgtccag
     1621 ttgaaacgcc agaaggacaa aatattgggc ttattaataa cctagcgtgt tatgcgaaag
     1681 ttaatgaata tggctttatt gaaacaccat atcgtaaagt cttaaaacca tatggtcaaa
     1741 ttacaaacga aacaatttat ttagacgcgg acgaagaaaa agagttcgtt attgccgaag
     1801 caaacgttga agttgatgaa aatggtgtga ttcaaactca agaaatcatc gcccgttatc
     1861 aaggtgaaaa cgtcttagtt agtcccgata aagttgatta cattgatgtc tcaccaaaac
     1921 aaatcgtgag tatcgccgct gcgtcaattc cgtttttaga aaatgacgac gcaatgcggg
     1981 cactcatggg tgctaacatg caacgacaag cgctaccatt aatggtgcca gaatcaccat
     2041 atgttggtac gggacttgaa acaaaaattg ctcacgactc ggggtcagca attactgcac
     2101 ataaagatgc cgttgtcact tatgtagacg gcgctaacat tagtgtgaag tttgatgacg
     2161 acacaacttt cacttataaa ttacaaaaat tcgcgcggag taaccaagga acttgcttta
     2221 accaagtacc aatcgttaaa gttggtgata aggtgaaaaa aggtcaagtt ttagccgatg
     2281 gaccatcgat ggttaacggt gaactctccc ttggtcgtaa tgttactatc gcctttatga
     2341 cgtgggatgg ttacaacttt gaagacgcgg ttattatgag tgacagactc gttaaagaag
     2401 atgtgtatac gagtatccat attgaagaat ataaaattga agcccggaaa attaaaactg
     2461 tttctgaacg gattacgcgc gacattccaa atgtgagtga agccgcaaaa gcgttcttag
     2521 atgaaaacgg tattattatt cctggtgcgg aagttaaaga aggcgatatc ttagttggtc
     2581 gcgtgacacc aaaaggtcaa cgtgaacaaa gtccagaaga tcgcttacta caaacaatct
     2641 ttaatgaaaa agtgcgtgaa gttaaagaca cttccttacg tgtcccgcat ggtggcggcg
     2701 gaacggtgat tgacgttaaa gtctttaccc gtgaagcagg cgacgaacta ggaccagacg
     2761 tcgaaattag tgtcagtgtc tttatcggcc aaaaacgtaa aattagtgaa ggggacaaaa
     2821 tgagcggacg ccatggtaat aaaggtgtta tttcgcggat tctcccagaa gctgatatgc
     2881 cgtttatgga agatggcaca ccaattgata ttatgcttaa cccccttggt gtaccaagcc
     2941 ggatgaatat cggtcaagta cttgaaattc acttaggtat ggcttgtaag aaactcggtg
     3001 ttaaagttgc ttctccagta tttgacggga tgactaatga agaaatcttt gaattaatgg
     3061 aagaagcaaa gatgcataaa gacggtaaag aaatactata tgacggccgc accggtgacc
     3121 gttttgatga acggattagt gttggtgtca tgtacttaat taaacttgtc cacatggttg
     3181 atgataagtt gcacgcgcgt gcgactggac cttatagtct tgtcacacaa caaccacttg
     3241 gtggtaaagc gcaaaacggt ggacaaagat ttggggaaat ggaagtgtgg gcacttgaag
     3301 cttatggtgc cgctaacatt ctccaagaaa ttctcacaat caagagcgat gaccgtgttg
     3361 gtcgtaaacg cacttacgaa gcaatcgtga agggctacga cttaccaaca ccaggaatcc
     3421 ctgaagcatt taaagtgtta gtgcgtgaac tacaaggact cgcgattaac gttgaattac
     3481 ggatgaaaga cggcgaaatc cttgacttag ataaactcgc taaagagcaa gatgcagata
     3541 tccgtcgctc ccgcagaagt actgctgtta gagaagaaca agaagtgttc gacccagaag
     3601 aaacagcagt ggtctacaaa aatgaagaaa gaggggatga ataattatgg caagaaaaca
     3661 accatttaat attaatgatt tagcaagtgt ccaaattgga cttgcttccc cagacatgat
     3721 tcgggaatgg tcaaatggtg aaattttaaa accagaaacg gtcaactacc ggagccaaaa
     3781 acccgaactt gatggtcttt tctgtgaacg aatcttcgga cctagtaagg attatgaatg
     3841 tcattgtggt aaatatcgac ggattcgcca ccgcggtaca gtttgtgaaa agtgtggtgt
     3901 tgaagttatt tctaaatatg tgcgtcgtga acggatgggg catattgaac ttgctagtcc
     3961 gtgtacacat atttggtact taaaagggac accggcaaga attgcaaccc ttttagatat
     4021 ccctgctaaa caactagaag aaatcgtcta ctttattagc catatcgtaa ctgatccagg
     4081 cacaagtagt gtcctttctt acaaacaatt tattgatgaa acaacagggc gtcaagtgtt
     4141 cgctgaagcg attaaagaaa ttcaagaaaa tattgaagaa ggtagctttg aacacgcaaa
     4201 aggtagtgaa cttttagaac gacttgaagt gacacaaact ttcgacttct ttgcgacttc
     4261 gcgttatatt agtaaacata ctggcgctaa gtttggtgaa ggtgcggaag tcattaagca
     4321 actcttaaaa gaagttgact tacaaaaaga atttgatgaa gttaacgctg cacttaaaac
     4381 cgaaagtaaa acaaaagaaa agaaattatt acgacgctta gaagtaatta atgctttcct
     4441 taaatcaggt aaccgcccag aatggatggt gcttgatgtt attcccgtaa ttccaccaga
     4501 tttacggcca atgttacaac ttgatggcgg cagatacgcg actagtgaca ttaacgactt
     4561 atatcgccgt gtcattagcc ggaataaccg ctataaagcc ctagtgcaaa tgaatgcgcc
     4621 aaaagttatt ctcatgaacg ggcaacggat gctccaagaa gcagttgata gtctcattga
     4681 taacggccgt cgcggcaaac catacactgg cgcgaataaa cgtccactta aatcattaag
     4741 ctcaagctta aaaggtaaac aagggcgttt ccgtcaaaac ttactcggta aacgcgttga
     4801 ctatagtggg cgtagtgtta ttgctgtcgg tcccgaactt aagatgtatg aatgtggtat
     4861 cccgcgcgaa atggcgattc aattattccg cccatttatt gctgcagaac tcattaaaag
     4921 agattcacgc gccacaaatc atacacaagc cgataagatt attgatcgtc atgatgaaat
     4981 tgtcttcgat attattgaag aaattatcgc tgatcaccca gtattactta accgtgcacc
     5041 gacgctgcac cgtttaggga ttcaagcgtt ccaacctgtg cttgtcagtg gtcgtgcgat
     5101 tcgtttacac ccacttgtgt gtgcgggctt taacgcagac tttgacggtg accaaatggc
     5161 cgtccacgtc ccgcttagtg aagctgcgca agaagaagcg ctaaacttaa tgcttgcgag
     5221 caaaaatatt ttaggaccaa aagatggtaa accaattgcc accccgtcgc aagacatggt
     5281 gattggtaac tacttcttaa ccgttgaaga gtcacgcgaa gactttgtgc gtcgcgccga
     5341 agaagcacga gcgttaggcg acgaatttgg tgcgcaaaag tttatgcaat atgctgaaag
     5401 cgaagggaaa gtatttggtt cgattgatga attaatggtt gcttatcaaa caaatagtgt
     5461 ccacttacat aaccgtatcg cgcttcctgg ccgagcattt aacaaaacat tatttacacc
     5521 agaaatgaat aagtcttatc ttgtgacaag tgttggtaaa gtcattttta atacaatttt
     5581 ccccaatgac ttcccatact taaacgaacc aaataaaggc tcattacaaa tggataaaat
     5641 tgaatacttt gtgcccgctg gtacaaatat tcgcgaacac attgcaagtt taccgctgcg
     5701 taaacctatt aaccaagaat tcttaggttt aattattaat gaagtgttta cgcgttatgg
     5761 cacggtgaaa actagtgcaa ttcttgacaa aattaaagac caaggtttcc attattcaac
     5821 aaaagctggt cttactgttt cactccacga cattacccca attgaaggta aagaagaact
     5881 cgttgaagct ggtgaagttg aagtgcggaa aattaataaa gccgcagcgc gtggattcta
     5941 tagtgaaaaa gaacgctata atgccgtcat taatgtctgg aaaggtgtta aagatgacgt
     6001 tgaagctaaa cttaaagaac agttcccact tgataaacgt aatccgctct acatcatgtt
     6061 tgatagcgga gcaagaggta aaacaacaaa cttcttacaa cttaaagggt tacggggctt
     6121 aatggcttcg ccgcgtggtg gcgaaattga aattccgatt aaatcaaact tccgtgaagg
     6181 cattagcgtg agcgaattct tcattgcttc gcacggttca cggaaaggtg gtgcggatac
     6241 cgcgattaaa actgctgact cagggtactt aacccggcgg ttagtcgacg ttgcccaaaa
     6301 tgttatcgtc accgaagaag actgtggtag tgatcacggc tttgtcgtta ccgaaattcg
     6361 tattcgtgaa aatgacaccc cagcagtgcc acttagtgac cgtattgcgg gtcgctatac
     6421 agtccatgac atcattcacc cgacaactgg tgaagtaatt attgaagcga atactcttat
     6481 taataaagac gctgcgcttt atattgaaaa agcgggtgtt aaagaagttg aaattcgttc
     6541 gctctttgga tgtgaagcgc caagcggaat ttgtgcgcac tgttacggca caaacttagc
     6601 gactggtgaa cgtgttaaag ttggtgaagc ggtcggaatt atggcggcac aatcaattgg
     6661 tgaaccagga acacaattaa caatgagaac gttccacagc ggtggtgttg cgcaagttga
     6721 agacgtcacc caagggttac cacgggttca agaattattt gaagcccgta atccaaaagg
     6781 taaagcaata atcaccgaaa ttgctggtga agttgtaagt atcacgcaag aagaagaccg
     6841 tcgctatacc gttactgtta aaaacgattt agaagaaaag agttatttaa cagagtatgg
     6901 tcaacaatta cgtgttaaag aaggcgacac acttaaacaa ggggaccgca ttactgaagg
     6961 tgctgtccac ccgcgtgaac ttctcgctgt tagcacaatt accgcggtgc aaaactattt
     7021 acttaacgaa gtacaaaaag tttaccgcat cagcgcttct gtccgcatta gtgataagca
     7081 cttagaaatt attattaacc aaatgctcaa ccgcgtttat attattgatg gtggtgatac
     7141 gagtctctta ccaggacaaa gaattacgac ccatcaattt accgcggcga ataaagaagc
     7201 acttattaac ggactccatc cagctgtagc gacaccatta attcttggta ttaaagctgc
     7261 tgcgcttgca agtgactcct tcttaagtgc cgcaagtttc caagaaacaa cacgcatctt
     7321 aactgacgcc actgttaaag gtaaacttga cccactccac ggcttaaagg aaaacgtcat
     7381 tactggacgc ttaattcctg ctggtcgcgg tatcttaacg aaagaagagc aagaggcctt
     7441 gcttgaagac tttagtgtcg ctgacacttt agacaaaatt aaagcaagtt acgacaatag
     7501 tagtaacagc taaataaatt aagggagcaa tataaaaaca tattgctctc ttttagtaac
     7561 cggtttcata taaatatatg aaccttatca tttaactagt aattagagca tgatataatg
     7621 ccaaagccat taaaaagaaa ggatggtatg attatgaaaa aaagattact attattacca
     7681 ctattagcaa tgcttctagg tgggtgtaac ggcggaagta gtgctactcc aagcatcagc
     7741 accccagaaa gcattcctag tagtgaagaa ccaagtgttg aatcaagtgc agaaagcgtc
     7801 agcgtttcaa ttgatacgac gagctatgaa atagcgacaa cgacaaatta ttcaaaccgt
     7861 gaattagatc attcaattga tatgactgca ttagactcac ttggcacaca aaagatgtta
     7921 gtcgttcctg tcgtgtttag tgacaccgcg caccgcgcaa ctgaagaagt ccatacgatg
     7981 attaataaag ctttctttgg tgcaagtgaa gaaacaggct gggaatcagt cgcctcttat
     8041 tatcacaaaa cgagttttgg aaaattaact ttaactggtg aagtactccc ttacttctat
     8101 tcacaatggt caacccatgc ttttggcgca ttagaagttt ccccgacgca agaatacggt
     8161 gaaggttatt actgggatca aacgcaccac ttaattgaag aaatttacaa tacattagat
     8221 gccgaactat tcttagaata tgaccaagat ggcgacggcc acgttgatgc tttctggatg
     8281 gtgtatattg ccgagattaa agcattaggc ggggaagacg atccgttctg ggcttataaa
     8341 ttttactgga accgttacgc taataccgaa aaaccaacac caaatactta tgcgtgggca
     8401 agttataagt ttgctgacga aggtgtcggt tataacttca atattcctga tgcgcatact
     8461 tatattcacg aaacaggaca tatgtttggc ttacctgact attacgatta cgattcaaaa
     8521 accgcaccag cgggcgcgct tgatatgatg gatcataacg ttgccgatca caatatgtat
     8581 tctaaatatc gcctcaactg gtcaaaacca tttgtcgtta ctggtaatgc ggatattcat
     8641 ctccgtccag cggaatcaac cggtgacttt atccttatta aagacggatg gaacggacat
     8701 gtgtatgatg aatatatttt aattgaatac tataccccaa ccggacttaa tgaaaaagat
     8761 agtcaaggga gggggtatga aacaaaatca agtaacgtat cgacaaaaag ctttgatatt
     8821 aatggggtga gaatgtggca cgttgattca cggctcatga cttatgtctt cgatcctgaa
     8881 tcattagaat taacaaatgc attttggact gatgaaattg ttgttggtga tccatttagt
     8941 tacactatta ttggtccaag taatacaact tcacgtagca gcacaccaga aggcgatagc
     9001 aaggttaata agttattaca cttaatggac gctaccggta caacttcacg tgttggtaac
     9061 tggttacgga caccaaccac tgcgggctca acagcgttat tccaaactgg tgatgtcgtc
     9121 gccgctgatg ggtggaagaa atatatccaa acaagcgcaa catttaacga cggcacagca
     9181 gtgggttaca gtgtcgagat tggtgaaatg gatgataacg gtgtccaaat catcattcgt
     9241 aaagcttaag ctactgctta acaaataagc gagttcattg aaaaacgcat acatttctct
     9301 taaaatataa ggatgaaatg taaagttaaa agaaacacaa agaaggtcac cttactccta
     9361 agtctcttgc tacttagtag ttgtaatttg gaggtgacct tttttcctta cacacaccat
     9421 ataagtgaca ctgatactta tacaaggatc gaaacaagtg attattataa agttggtgct
     9481 tttgcgcgca gttatcaaga tgtattaaaa agtaatttaa atggtaattc acgtgttgta
     9541 ccacttaaga gtagtggcga acaaaagatc cttgttttgc cggtgaattt ccctgattat
     9601 gacttaaatg aattagatgg taataatggg aaaaccgcgc atattcatat tcaaaatgct
     9661 ttctttggtg aaaatcacac aacacgcttt tattcagttg cggggtatta cgaagcttct
     9721 agttacggca aacttaaact tagtggtgat gtggcgccgt ggtacacttt acccgctgaa
     9781 tatagcgtta gcgcgattaa aagcagagta cggagcaaca gcgataaact taatgaaacg
     9841 gcagaaatac ttaatttagc gctcaaagaa tacgctaaaa gtaatgactt aagtgcgttt
     9901 gatttagatg aaaatggtgt gcttgacagt gcttatgtaa tatatagtta tccgtttgaa
     9961 ccaaaagatg tgaataatat cttctgggct tttgccgcga acatgaataa aaaagatcca
    10021 gaacttaccc atcaagcgaa tgcttatagt tggagtagtt attattattt agatgtagcg
    10081 cgcaataaaa aacctgatcc ccatacttat attcatgaag taggccatct ttttggttta
    10141 cctgattatt ataatacgaa ttatgacgat ccttattccc cgcttggcgg ctttgatatg
    10201 atggattata cgcttggtga tcataccggg ttatcaaaga tgttacttga ttgggcgcgg
    10261 ccttacgcct taaaaagaag tactacaatt aaacttcgac cctttagtga aacaggtgac
    10321 ttactcgttc ttaaaaataa ctggaacgag aaagcaaatg atgaatatat tttgctcgaa
    10381 tattacacac caacagtgtt aaatgagctt gattcaacgc ttaatgcttc ctttaaactg
    10441 ccaaagacaa acggagtgaa ggtgtatcac gttgacgcga gaacggtgta cgaaattaaa
    10501 aacgggagta ttgttaagta tccttacgtt aatgattatg aaggagaatt taacggtact
    10561 gaacttttag cgcatagcaa tagtactgga agttataata atgcaccaat taagacgaat
    10621 aaactttata aattacttga gccaaaagaa agcacgaaca tcagcgagtc gtttaatcaa
    10681 gcaaatcagc actctttgtt tcaaaaaggt gatgagtttg gctatgaata ttttaaaaat
    10741 ttcgcattta atgataagac accgcttaat tttcgcttta aaatagtaca attaactaac
    10801 gcttatgcga caatagaaat agaggtatat taagatgaga aaaagtacat ttatccgtcg
    10861 agcattaatt ttagtagtcc cagctctact cttaacaagc tgcgtgactt ttggtgatat
    10921 attaaagtcg tccgagtttg gtgcgactag ttcaagtgcg aatgaagtaa cttccgaagt
    10981 aactggcagc gaaaatccct atcctttaaa cgggacatac ggcgttaatg attatgaaca
    11041 cccaattacg gcgaatccag atactttaaa taattattat aaagtagaac cgcttaacaa
    11101 taattcgcga ctaattaata actacgctat ttctaatacg atgggtttag cgcaccttga
    11161 ttcagtttct aaccaaaaaa tgacggttgt acctgttaaa tttactgata cacccgcaag
    11221 ttacggggga gaggcgacta ttcagggagc gattaatcgc gctttctttg gtgaaagcag
    11281 tgatacaggt tgggaatcag tagcctctta ttactataaa tcaagttatg gtagactcag
    11341 tcttaccggt gaagtaatgc ccctttttga tatgggttat tccactactg aattgttagc
    11401 gaaaactgtt gacggttatc agtactggga taagagtcac tatgtgcttg aacaggtgta
    11461 caacacttat ctttcatcaa cattaaaaga atacgaccaa aataatgatg gctttgttga
    11521 ttccgttttc tttgtgtact tagcacctgt tgattatgaa agtgatgata cacttttttg
    11581 ggccttccaa tatttttgga atagatttgc cgataaaaac aaaccagtat ttaatgctta
    11641 cggctgggcg agttatgaat ttatggttga agcgccaggt tatacatatc ttaaaccagc
    11701 agcgcatact tatattcatg aaagtggtca tatcttaagt tttgatgatt attatgatta
    11761 tgacggcact atagcgccag cgggagcgat tgatatgatg gatcataata ttattgatca
    11821 caatatgttt tctaaattta gcgcaaactg gagcacaccg tatgtcccgg ttggtaatag
    11881 tgatataagg cttagacccg ctgaatcaag tggtgacttt atcattatta atgacgcttg
    11941 gaatggacat gcttatgacg aatacatttt gattgagtac tacacaccaa ccggtttaaa
    12001 tgcgcgtgat agcttatcag tttcaggcta taacggtgtc aaaggttaca cgattcctgg
    12061 ggtacggata tggcatgttg atgcgcggtt agtgattttg tcaaataacg gtaatactaa
    12121 aaagtggagt gatacaattc cgccgcactt agatggtagc ggtggttcat ggatggattt
    12181 tgctacttcc aatacaccaa gttataatta tgcgcataac agtttacata aattgcattt
    12241 gcttgatggt cgcggacgcg cgcaaacatg ggcaaataat ttagtaaaag cggacgactc
    12301 agcgctattt caaacaggga cagttattgc tagtaataat tggcaaaagt acttgttaaa
    12361 aagagcaacc tttaatgacg gtagcgtaat tggttatagt attgaaattg gtgaaatgaa
    12421 tagcgaaggg gttaccatta aaattcgtaa agcttaagtt gtcgttttgt attgtaattt
    12481 ttattaatca aataatgata aaataaaaca tatggctaca ttaaaagata ttgcaaaagc
    12541 tactggattt tctgttatta ctgtttcgcg tgtcataaat tcaccgcatt tagtgaaaga
    12601 aaaaacacgg aagattattg aagcagaaat agcgcggcaa aattttaagc cgaacgtttt
    12661 agctagagca ctcgtccaaa atcgcacgaa tatcatttat gtgtatattc ccgctgatat
    12721 tgatgcgatt aacccgttct ttttacatgt tgtgtctggt ataggcgaag tacttggcga
    12781 aaaaggtttt tccttactct taaaaagatc gtggtatgag ggcgaagatt gtgatggctg
    12841 tattttcatg ggacttagcg cggaagatga agcaaaggta tttgaacttt caaaaactaa
    12901 accagtgtgc gtctttggct atgtagaagg agtgtcaagt gttgacgttg ataattatca
    12961 agggctttac ttaatgacaa gtcacgtact tagtaaaggc tatcgaacac tgacatattt
    13021 aggaattgat gactatagac ggtttatctt acagcgtaaa aaaggattta gtgacgcagt
    13081 tatgagtcaa aaagataaaa ttactgtaaa atatttggcc gctaaaaata atgaaaatga
    13141 cgcttataaa gtcgtgggtc aaagtttaaa acaaggtgat actcctgaag taattgtgtg
    13201 cgctacagat gatatggcaa ttggtgctat tcgcgcaatt aaagaaaaag gtttagaagt
    13261 accgcgtgac atagcagtaa ctgggtttga tgggttaggt acccaagaag tagcgcaccc
    13321 tcgacttact accattcatc aaccaattta cgaagccgga aaagcacttg cccatcgcat
    13381 tattgccctg attgaaaata aaaatgaatt accagttaat cacgcattca ttgaaacaac
    13441 gattattcat cgcgaatcaa tctagctatt acatagaatt tcaatttaac ttgcaaaatt
    13501 agcaattata ttttaaaatg ataacgataa cattttatag aggtgcacaa aatgaaaccg
    13561 aagaaaataa caacaatctt atttagtctt tttttggctt tatcactcag tgttgtgact
    13621 ggctgtaaag atggtagtga aaatggtggg agtgatgacc caattaactt ttgggcctat
    13681 cagccttcaa aacaaacaga ccaaacagcg tataaaaatt taattgctga ctttacagcg
    13741 gaaacaggaa ttaaagttaa attactgatg gtcgttaaag atagttataa caaatcactt
    13801 aatgcggcga taagttcacg cagtaaacca gatttagctt atttcgatca accacggatt
    13861 agtgattatg cgcacgacga cacgattgct aaactagacg aatatatcgc taatagtgcg
    13921 gttattgata atactaaatt ctttgcttca ggtttagcga caacggtgta taaaggtagt
    13981 cagtacggtg tgccactgaa tatgacaaca tcagtgcttt tttacaataa agacatcgtc
    14041 acgaccccgc ccactagttg gcaagaatga cttaacaccc cagtaagtgg acataatcag
    14101 tcactatttg acgggattgg cactggagga tatgcgggat ggttcactga ccctgaatta
    14161 acgattgctt atgttccggg tctattaact gctgatttaa cactctacgg aaaatacgtt
    14221 gaatcacaag atactacttt aatcttctat gacccgaata aagttctttc aagtggtgac
    14281 gtttatcttt atacatggga cggtgatggt cgcaaaataa caggcaattg gcctggaaca
    14341 gcaatgttta atcacggatt cggctactat tcatggtttt tccatgctgc ctatcttacc
    14401 gaaaacgtta tattccacga taatgctgga aatcaaacgg gcgatatagt gtgggaaatt
    14461 ggtaaaaatg tctatcatca agaaacagga acatggactt ttgttagtga agttcatcat
    14521 aaagctaccg tatacgcgag taagattctt gaaaaaacgg caacatgtga cgcaacaggt
    14581 gcagcaaata atttaaatgc accagaatgg aatgaaactc tagcgaatca atataatgac
    14641 tcacttgatc ctacaacaaa agcgtattta aaaacagcgg ttgctgattc taatggtgac
    14701 ttattagcgc aagcaatggc gcgttatgac tatattgtga agaagtatgg aaccgcaatg
    14761 tttagtaact tccttgaccg gacaatccct gctagtggat ctccgcttgt aagtaatgaa
    14821 ggcacaaaca atgcactggt aatgggggca tttatcgtta tggcattagt tgctcttgct
    14881 ggctttaaat taatcagtaa aaaacatcgt tatcactaaa ttataaaaca acgttttatg
    14941 taactaaagg ctggggtttg actccagcct ttttaaacat taataaaaat aggttgaatt
    15001 tatcctaata ataatctata attatcctga gggtgcgata atgattatta aaacagagca
    15061 aattattaca atgactgatg caaatcaaaa tttttcaaaa gcttcaaaaa ctgcggaaaa
    15121 atatggttcc gctgtgatat ttaaaaataa caagccaaaa tacattcttg cagatattga
    15181 acaatatatt gagttaacag aagacgaaaa gatagaaata gttgcgaaaa gaatccttag
    15241 taaacatatc aacgccttta aaaatttagc aaaatgatta aatttactaa agaacgaata
    15301 ttattctata taaactcata attgaggcca caggaggagt ttttggtatt cgtgatgaga
    15361 gtctactaga atcagcaatt gaagcaccat atcaaacttt tgatggtgtt gaacttttcc
    15421 cgactaaaat tgaaaaggca gctagacttg gctttggatt tatttcaaat catccgtttg
    15481 tggatggtaa taaaagaatt ggcttatatg tgatgctttc ttttttacaa ataaatggga
    15541 ttataatttc ttttactgat gacgaaattg aacaaatagc ctttggaatc gcaagcggag
    15601 ctattaacta tgttgattta aaaagaattc tcgaacaaaa aattgataat taaacatatt
    15661 aagcattata attcacatta aaaatcatat agttaattta tattttaatt gcatattctt
    15721 aacaacggat tttactttgt caattaacaa tctcgaataa ttttcagttt tgtgttgaca
    15781 ttatttagca atgtccttat aatatcaaag tgcaatgacc taaaggtcgt ttttaaaata
    15841 aaaaccttga tacacccggt tgtgtgtcga gtagcagaaa agaagattcg aagaaaggag
    15901 aaagatggat gccaacaatt aatcaattag tgcgtcatgg ccgtcaaaag gcagtctaca
    15961 aatcaaaatc acctgccctt accaaacgct ggaactcact taaacgtcgt gaagttaatc
    16021 agtattcttc acataagcgt ggtgtgtgta cccgggttgg aacaatgaca ccaaagaaac
    16081 ctaactcagc gttacgtaag tacgctcgtg tacggttatc aaatggttat gaagttacag
    16141 cctacattgg tggagaagga cataacttac aagaacacag caccgtatta attcgtgggg
    16201 gtcgtgttaa agacttacca ggggtccgct atcacatcgt gcgcggtaca ttagacaccg
    16261 ctggtgtgga aaaaagacgt caaggtcgta gtcaatacgg aacaaaacgc ccacatactg
    16321 ctgaacatta ataaaaaaat aaacaaacta cttattatcg aaggaaggag gaaacgatat
    16381 gcctcgtaaa ggaagtgttg caaaacgtga cgtcttacca gacccaattt atcactcaaa
    16441 gctcgttacc cgcttaatta ataaattaat gtatgacggg aaacgcggaa cagcccaaac
    16501 gattctttat aacgcttttg aacgtatcga agcgaaaaca aaacaacctg caatggacgt
    16561 ttttgcaact gcaattagta atattatgcc tgaggtcgaa cttaaggttc gtcgtgtcgg
    16621 tggccaaaac tatcaagtcc cgactgatgt gagcccagag cgtaaaacaa cattaggtct
    16681 tcgttggtta gtgaactatt cacgcttacg caatgaaaga acaatggaag aaagactcgc
    16741 agcggaaatt attgacgctg ctaatggaac aggcgcgtcc gtcaaacgtc gtgatgacac
    16801 ccataagatg gcggaagcta acaaagctta tgcgcattat cggtactagg aggacccatg
    16861 gccgatttaa ctaaatttga tttgcaacat acgcgtaata tcggaattat ggcgcatatt
    16921 gacgcgggga aaacaacgac aacagagcgt attctttact acactggtcg tttacataaa
    16981 gtcggtgaaa cacacgaagg tgcatccgca atggactgga tggcccaaga aaaagaacgc
    17041 ggtattacaa ttacaagtgc ggcgacaacc gccttttgga aagaaaaccg cattaatatc
    17101 attgataccc caggacacgt cgactttaca gttgaagttg aacgttcatt acgtgtactt
    17161 gatggtgctg tcacagtatt agatggtaaa agtggtgttg aaccacaaac ggaaactgtg
    17221 tggcgtcaag gtacaaaata caatgtccca cgcatcgttt ttgtcaataa aatggatgca
    17281 actggcgctg atttcaatat ggcggttagc acgcttcata gccgacttgc cgcaaacgct
    17341 gaacctgtgc aaattcccat cggtgttgaa agtaacttcc gtggaatcat cgatattatt
    17401 gaagaaaaaa catatattta tgatgataat atcgctaatg tttcaatcga agaagttccg
    17461 gaagaatacc tagaagaaat gcacattgca aaacaaaact taatcgaaaa acttgccgcc
    17521 tttgatgacg acttcatgat tcaagtctta gaaggcgaaa ctccaagtgt ttcagaaatc
    17581 aaacgcgtta ttcgtaaagc cgtcttaact ggtgaattct tcccagtatt ctgtggtagt
    17641 gcttataaaa acaaaggtat tcaaccactg cttgacggcg ttgtcgatta cttaccaagt
    17701 ccgttagatg tcccgccggc aaaagctgaa ctacttgatg gtactgaaat tattacccca
    17761 agtgacgctg atgcaccact tgcggcctta gcctttaaaa tcgcgaccga cccatttgtg
    17821 ggacgcctag cgtatgtccg tgtctattca ggtacgatta aagctggatc ctatgtgtat
    17881 aacagtatta aaggcgttcg tgaacggatt agtcgtttag tgctcatgca cagtaatcac
    17941 cgcgaagaag taagtgaact taaagctggt gatattggtg cggttgtcgg atttaaaaac
    18001 acgacaacag gggatacaat ttgtgatgaa aagaataaat taatcttaga acgcatggaa
    18061 ttccctgaac ctgttattga acaagcaatc gaaccaaaga cccgtgctga ccaagacaaa
    18121 atgacaaatg gacttgctaa gttaatggaa gaagatccaa catttaaggt gacgactaac
    18181 cccgaaactg gacaaacaat tatcgctggt gttggtgaac ttcaccttga tgtcttagtc
    18241 gacagattac gccgtgaatt tggtgttaat gttaatgtcg gtgccccaat ggttggctac
    18301 cgcgaaacaa ttagccaaga ggcggaatgc gaaggcaaat atattaaaca atctggtggg
    18361 cgcggacaat atggtcatgt gtggattcgc tttgaaccaa acccaggcaa aggtttcgaa
    18421 tttgtcgatg caattgtcgg tggtgtggtc ccgcgcgaat acattaagcc aaccgaagaa
    18481 ggtttaaaag acgcattagt gcgcggttta gtcgctggat atcaagtaat tgatattaag
    18541 gctacactgt atgatggcag ttatcacgat gttgatagta gtgaagctgc ttacaaaatt
    18601 gctgcatcaa tggcgcttaa agaagcggga cgcctagcag aacccgtctt acttgaacca
    18661 attatgaaag ttgaaattac tgttccagaa cagtattatg gcgacgtgct tagtgactta
    18721 gcaagacgcc gcggttcaat gaaggatgaa agccaacgcg gaaacgctaa agttattgat
    18781 gctgaagtac cactttccga aatgtttggc tacgttactg atttacgtag tttcacccaa
    18841 ggtcgtggta catatacgat gacttttgat cactatggcg aatgtccacg ctacatcgca
    18901 gatgaaatcg tcaaaaagag tggaactagt gctcgctagt ccgcaaatgc tatataaata
    18961 gcacaatgat ttgataaaaa attaaaattg ctataaactg atagcgttgc aataaattaa
    19021 aacgctacaa gttaggagga aattattatg gcaaaagaaa gatttgacag aagtaaagtc
    19081 cacgttaacg ttggtaccat tggtcacgtt gaccacggta aaacaacctt aaccgcagct
    19141 attaccaaag tcctcgcatc gcaaggcggt gcaaaattaa tggcgtatga tcaaattgac
    19201 cgtgcaccag aagaaaaaga acgcggaatt acaattaata cttcacacat tgagtatcaa
    19261 accgccactc gtcactatgc tcacgttgac tgtccgggac acgctgacta tgttaaaaac
    19321 atgattaccg gtgccgcaca aatggacgga gcaattctcg ttgtggccgc tactgacggt
    19381 gttatgccac aaacacgtga acacatctta cttgcacgtc aagttggtgt tccttacgtt
    19441 gtagtcttct taaataaagt tgacttagtc gacgacccag aactcgttga catcgtcgaa
    19501 atggaagttc gtgaattact taacgaatac ggattcccag gcgatgatac cccaatcgtt
    19561 cgcggcagtg cccgtgatgc attagacggt aaagcagatg ctgaaaaagc aatcatcgaa
    19621 ttaatggaag ctgttgacag ctacattcct gacccagaac gcttaaccga tcgtccattc
    19681 cttctcccaa tcgaagacgt catgaccatc agtggtcgtg gtaccgtcgt cactggtaga
    19741 gtggaacgcg gaatggtcaa attaaacgaa ccagttgaaa tcgttggtat tcgtccaact
    19801 aaatcaaccg tcgttactgg ccttgaaatg ttccgtaaat tactcgacag cgcccaagct
    19861 ggtgacaacg ttggtgtttt actccgcggt gtcgcacgcg acgaagtcga acgtggtcaa
    19921 gtcttagcca aacctggctc agttaaacca cacacaaaat tcacaagcac cgtttacgtc
    19981 ttaacaaaag aagaaggtgg tcgtcacact gcattcttat caaactatcg tccacaattc
    20041 tacttccgga caactgacat taccggtgtt gtaacactac cagccgatgt gcaaatggtt
    20101 atgcctggcg ataccgtcac cttaaccgtt gaactcattc acccagtcgc cattgaaaat
    20161 ggaactaagt tctccattcg tgaaggtggc cgcactgttg gtgccggtac tgttaccgaa
    20221 atcttaaaat aagatttaaa gcattacaca actaactcct cttcagagga gttttttgtt
    20281 tatgattata tcgtaacaat tgaaatatat gatagttttt agtagcatag tacttagcga
    20341 aatatgaaaa aagaaaagaa aattacccaa cctaatttag atacagttcc aggtggcgct
    20401 ttaaagcgta ctggtgcagt tttaattgac cttgtcatta tgtatttatt aatgaatgta
    20461 ttatggtttg ccattcatcc aatttataac aaagtttatg atattgataa tgttgtgacc
    20521 gcacatgagc gtgataaaaa acgtagtaac ttagtcgtcc ttaatgaacc gattcttgat
    20581 gatgaaagca atattgactt agttaaagtt gaaagtaatc aattaccaga aacaaaacgc
    20641 ggggaaggtg tctttaagtt ttacacagtc tttttgcgca gtgaacaaga agcgaaatta
    20701 gcggcgggtg aaattgataa tacacctgaa aataatgcaa tgtttacatt tgattggtac
    20761 ttagaaaatg tcattaaacc tgatacggaa gaatcaattt atttacgcat tcccactgat
    20821 gaacatttag gagcagaagg cgcggttagt agtgaagaaa ctgctgaacc ggagtacgac
    20881 acttttcgtc cagcgggatt agattttaaa gcggatttaa ctgcggaaga tattaataaa
    20941 tttaataacc gcatttacaa tcaagcagtg aacgctttta atttacgacc gaatatgaca
    21001 atcattaata aggttaatat tcaggaaaat ataatcttga tggtgcttgg tagcactgtc
    21061 gtctttttag tgttcccgct tttctttaaa cacggtcaaa cactcggcaa aaaggcctta
    21121 aaattaggag taacgacgcg tcatggttac acagttactg tgccgtggtt attacttcgt
    21181 tactttgctt tcttatttat taacttatta agtaatctct taattccaat tattttaccg
    21241 tttatctcac tcacaattat gacctttagt aaagagcgtc gcagtgcgca tgactttatc
    21301 gctaatacga aagtaattga tttacaaaat tcgacagtat ataaaagtga agctgagttc
    21361 ttagaagcac aaaaaagata taatcctaga cttgaggctg aagactttaa tgaagaagta
    21421 tttagtgaac gcttcgatga agaaagtggc gcctaatgaa ccattatgtc gtaaagcaaa
    21481 cagcaggtaa acgcttcctt gcgggtttcc ttgatacgat atttgtcatt atattggcgc
    21541 tttttttata tattccggcg cgcactatag tagacaataa tggcttaatc gaagtgtttg
    21601 ttaaaatcac acaaattcaa ttaaaaagcg gactatatga cgttgaaggg attgataatg
    21661 cagtacctta catcattaat gatgttgaaa agttccctaa agcactctac acattttacg
    21721 ttgatgaaaa tcttgaaaca ggcgaaattg aacgcggtta cgctgatata ttagtggaag
    21781 gtcatgaata taacactgca gaagattatt atgtcgttat tttagggaaa ggtcgcgaag
    21841 actctttatt tacttttgac gctattaata gtgaagcgcc gtgggaagtt ccttttaaag
    21901 aagggaagca agaagcggtg aagcaatttt acactgatga aatagaaaaa gcgcatcaac
    21961 atcttgatta tcatgaagta gtagttccgc tcattattaa agctgaacgg cttatttttt
    22021 atgcaatggc gggaagttac ttaacaagtg ctttaattct tattgtcatt attccaaact
    22081 ttaggaaaga taaagtaaca tttggtaagg taattacggg gaccatagtc ttaaatcgtt
    22141 taggatataa aatgagcgtt ttgcaggcaa atatgcgcaa tttcgcgata tttatcttta
    22201 gttttgtctt tttctttgta ccctttcatc tcatttcctt tgcgatgaca ctcttttcta
    22261 aaaaacaact tagtatgtat gatcagcttg tggcaacact cgtggcggat aaaaaagcaa
    22321 cgcttgtctt taaagatgta aatgaagaaa caaagtatcg taaagagctc gcacaacgcc
    22381 taattgccgt tgataaacgc aaagccgaaa gtcgtgaaga aagattacgc gataaagaga
    22441 agcttaataa aattgattaa aaactacata atgccgataa taaaacaata gccaaaaacc
    22501 ttgatattgt gctattataa aataaacata gcagacaatc tgcaggagga aatatggaag
    22561 ttagattaga taatctgtcg aaacacttcc ctggagtcaa aggcgctccg acagtcaaag
    22621 ctgttgatca gatgaatttt actattcctg atggaaagct tgtcggcctt ttaggacctt
    22681 cgggctgtgg aaaatcaacg acactctaca tgatcgctgg attacacaaa ccgaccgctg
    22741 gtaagatttg gttcggtgat cgtgatgtca ctgaggttcc gcctgaaaat agagggatcg
    22801 gtttagtgtt tcaaaactat gcgctctatc cgcattttac agttcggcaa aacattacat
    22861 tcccactcga gaatgtcaga ccgaaactag aaaaaaccga aatggagcaa attgctcaag
    22921 aagtcgcaaa cttagtcgac attggtaact tgctcgaccg cctaccaaaa caactttcgg
    22981 gtggtcaaca acaacgggta gcgattgccc gtgcgctctc gaagcggccc caagttttat
    23041 tacttgacga accgctctcc aacctagacg cacgcttacg gcttcaaacc cgtgaagaaa
    23101 tccggcggat tcaacgtgat acaaaagtta caacaatctt tgttactcac gaccaagaag
    23161 aagcaatgag tattagtgac tttatcgttg tgatgcgtcg cggtgttgtc catcaaatcg
    23221 atgcaccgca aacggtgtat gaagaaccag tcaacttatt tgttgcgaaa ttcttaggct
    23281 caccagcgat taatattttt gatggtgaat taaagaatgg taaagtatta cttcacggca
    23341 atgtttatca tgaagggctt aaatcaaaag gtaaatttga ttactttgat aaaagaattt
    23401 atgatatcgc tgttgaaggt ggttataacg gaacgaaaac tgattacttc gctagcttag
    23461 gcgttgatta tgttgaagca gagccagaag ttaaagaaga accagaaaag aaagcattta
    23521 aagaaaaagt tagtgatttc tttgccaaat taaatccaaa gaaaaaacaa aaatttgttc
    23581 cgcgtattat tgaaggtgaa gttaacattg aaagcattta tgaagctatc gtcggtggcg
    23641 gctatcgtta tgcttacgct gattttgaag aagaggttgg taaactcatc gttgaagctg
    23701 gtgcaaatcg tgatgtgaag attggtgtgc gtcctgaagc attcaccctt gacaaaaatg
    23761 ataatccagc aattgatatt gaagtacttt atattgaaca tattggccgc gatattacga
    23821 tcgtcgctaa tgttgtggga caaaaacacc ggattcgcgt tattatccct gctgaagaac
    23881 gtcataaagt caaagacaaa gtaattaagg tatatccaaa acgcttctat ctctttgata
    23941 ttgatggttc acggatttta taggaaaacg cgatgaaaga ttcttattta cgaaaggagg
    24001 taggctatga gcactaaagc aggagtactg catcttgaaa aagggccaat gcaaacgaaa
    24061 aagacgattg caatgccaat ttggttaaaa gcaataattg ctttattacc tgcccttttc
    24121 ttcttgcttg tctttaccat tttcccgatt tttaatacag catttattgc ttttgttaaa
    24181 gacttttatt tccaaagcgg gaccggtagt tttgcacttg caagttactt ccgacaactt
    24241 gacatgcata ataaccctga ttggtggcgc gaatattttg cgattattga acctgatgtt
    24301 ccctttttac aaccggttaa accaattatt ggttttgcga actttgaata tgttttaaag
    24361 gatccgcttt ttagtaacgc gcttattaat accgctattt taacaatcat ttccgtccca
    24421 ctgacgatta tgatttcact tttacttgcc gtatcgctta attccattaa gccgttacgc
    24481 gcaacttatc aaacaatttt cttcctcccc tatgtcacta acggcattgc cttagggatg
    24541 gtctttaata cgatcttctc gcatgaggtt ggggggatta tgaaccaatt tatccagttc
    24601 tttggcggaa aaccagtatc gtggattcgt ccgttatgga gtccagaagg aacgatcccg
    24661 ttatcaaaat tctccttagg atttgcgatt actgtccaat caatttggtc aggcttagcg
    24721 tttaaaatcc ttgtctttat ggcaggttta gcaagtattg acaaacaata ctatgacgct
    24781 gcgcggattg atggctcaag tcgcagcaca atctttagac ggattacggt tccactttta
    24841 agtccgcaaa tcttatatat tacgattact tcatttatcg gggcatttaa agcttatcaa
    24901 agcgttatct cgattgtggg gggcggacca attcactttg ggggcgaaaa ccgcacgatg
    24961 tggattacgg ttgtcgggta catttacatg aaccgcaacg ttgaaaagaa cattccactt
    25021 gccgcagcag gttctgtcgt cttacttatt gtaattttat tcatcacgct tattcagatg
    25081 caagtctcga agaagcgggt gcactattaa taggagggta gcgtaatgac agataaagat
    25141 aaaaaagtca cagaagcagt cgaattaact gccgaagaag aagtgaaatt atcacacatt
    25201 gatgacgaac gctttcagcc atcctatgaa atcgcaaaag aatataaaac gcgggcgatt
    25261 gttgttaaga ttattactta cacattctta acgctttttg ctttctttat gcttttaccg
    25321 ttctggttta tgattagtgt ttcagttaaa actccacttg aatatcaacg tgaagtgtcg
    25381 actggattac gcttatttac ggcaaatcca agttttgtta actttaggat tgtcttaggg
    25441 atggatttaa aagatccact aaaagagatg aatgaagcac tcttacatat cgttgaaact
    25501 tcaccagaac gcagttttat cccgtatttt cttaatacga ttatcgtcgc ggttgtctca
    25561 acggtcttta caattgtaac aacggtgctt gcctcgtttg cgtttgctcg tcttgaattt
    25621 aaagggaaaa acttcttatt tgcgatttta ctcgcaacga tgatgattcc tggcgaaatg
    25681 atgcttatta caaatattca aacagcgaaa tggttaggat gggaaaacac ttatcgcgca
    25741 cttatttttg tccacggcgt tagtgtcttc tacattttct acttacgaca aacattccag
    25801 caaataccaa acgaattatt ccttgccgcg aaagttgacg gctatggtga atttagctac
    25861 ttatggcgcg tgatgattcc aattggcatg ccgacaatcg ttacgattat tattctcaac
    25921 gttatgggta gctggaacgc ctttatttgg ccaacactta ttgcgcaagg ctataacccc
    25981 actattcaga aattatttaa atataaccat acgatgcgac ttgttagtaa cgggttaatg
    26041 tcgacattta ctggtcaatt cgctagttac gacaccgtta aaattgccgc atcgatgatt
    26101 gttactgctc cgttactgct cgtctttgtg ttcttccgta aatacattat gagcggtgtc
    26161 tcccgcagtg gtattaaagg ttagtaatta ctaatcgtta ttatataaac aagaaaggaa
    26221 agagagatat gaaatttaga aaactcatat ttacagcggt atcggtctta gctttaacaa
    26281 cgcttgctgg ttgtttacgc ccaccgatgc caccaacatc agaaacgcca acgacatctt
    26341 caggcggtgg tggtccgact gaaaaaatcg aagtgaagtt ctggacagga tttggggcat
    26401 cagttactgg tgcattaaac ccgcttttcg cgcgttttga acaagaaaat cctgatatta
    26461 aagtacttta tgaatcaaag agtggttatc caaacctcca acaagcgatt aacggttcaa
    26521 ttagtacaaa tacttatccg catatcgcta atggttatcc tgaccattta gtcggttaca
    26581 ttaacgctaa tattttagta aatcttggta accccactaa ctacatcaat cacccagact
    26641 atggtgttga tattaatgaa tacaatagcg actacatggc cgaaattaat ggcttatcag
    26701 acgctgcaac attcggctta ccgtttaata aatcaacgga agtaatgatt gctaaccaaa
    26761 gtttctttga tgcggctaaa aaaagagacc caagtatctt tattccaaaa acatggcaag
    26821 atttagccga agttggccca aaacttaaag ccgtcgcgaa agataacggc tggtttggta
    26881 aattagtact caaaaaagat ggaacagcgg tagcaaaacc tgcggaattt gatgcagcgc
    26941 ttaaagaaca ggtcgctgtt gatatgacgc tagttaatga aaatcaattt atccccttta
    27001 gctgggatag cgctagtaac ttctttatta caattattcg gcaatggggt gcaatgtata
    27061 cggaaaaagg taacacaatt caaaatggtt acatccgttt ccaagaagaa ccgcaccgca
    27121 ctaaaacact agcggcccta tcttatttta aagagctata tgaagatgga ttaattggtc
    27181 tacctgacaa ctttggtgaa ccgctttatt cttcaacacc atttaaagaa ggaaaactcg
    27241 tacttactat tagtagtagt gccggtgttg gtgaaaattt accaggcggg acaactgaat
    27301 atccatttga attatcgatt aatccaattc cttataacgg tgacttagca gcgcaaaaat
    27361 cagttattgc ccaaggcaca aacttagcgc tctttaccgt tggtcaaggc cgtgatccaa
    27421 aagcacaagc tgaacggctc gctgcctggc gcttacttcg cttcttaact tatgaagcaa
    27481 actacgaatt tggtaaagca acttcctact tcccagtgtt agacgcttcc aaaattgatc
    27541 gcgatagtca gcgttatcaa gattacttaa tgtatagtga attcttaaat caaacagacg
    27601 ggacaccacg cgaaaaagta attcgtagta ccgctaaact tcaggctaat gtgtatatgg
    27661 atcctgaagc aggctggaat aagttcgttg acccagggtt ccttggttca agtgaaattc
    27721 gtaataccat cgaatcagtg atggctgaat tatttaaagg taaaacaccg gagcaagtgt
    27781 taaatgacac tgttactaaa cttgcgcggt atgtacaaaa ataataaagg agaaatatat
    27841 gaaaaaaaca tttgttaaaa ttggcttgtt acttgcggtt tttggcttac ttgttggttg
    27901 tgataatagc agcagcgaag tagtgccacc ctcaagcgcc agtgaagaag tcgtttcatc
    27961 aagcgaagaa gaagtaaaac taactgagca agaatatcaa attgccgtta attacattgc
    28021 tcgcagtgtt ccgcttgtcc ttgaaagagg cgttaaagtt ccaacaaacg ttgaaagggc
    28081 tgaagcggag aacccactac cgacaccaga attaaagtat ggtgacaact tattacttat
    28141 taccgctggt gaattaaagt atgaagacga aattaatggc gtctatcttt atccagaata
    28201 tactgtaacc tattcattct tagagcaaga accttatgca ggctttactt ttgaatatga
    28261 tgatattggc cgttattttg ctgttcctga atatccgcgc tatgaagcgg aatatgacaa
    28321 tggcggtaac ccaattcaca ctgtaccacc ggcaaaagca gcgcggttat atgtcacgat
    28381 tcatattgaa gaacgtttca ctacatttaa tatggacatg attttagagc cacaagccat
    28441 tattcatgat tacaaccttg ctgatgtccg tagtgtgcca actggctcag ttgttagagt
    28501 ctacggttac tttaacggct atttcagtga ctggaataat ggtgtactgt gtgatggcga
    28561 tgtagcgtta ggtctcttta aacttcaaga atttaaccat atggaacttg gtaagatgta
    28621 cgctgttgtt ggtcaattca cggtgtataa cggcttacca caattacaat ggattaagac
    28681 tgtttctgaa gttgaacctg ctgatcaccc aacggttaaa gaaccaaaag ctgttgttgt
    28741 cagcgcagac gactttgctg atcaacttga tattggtaac gaattaagcg gaccacttat
    28801 gcataaagtt ggtacgacag tcgtacttaa cttcccactt cgttataagg caatgcttga
    28861 ccgtgatggt aacgccgttg aaccaggcgc attagaactt ggtaaacacg ctgatgttgt
    28921 tttaaccggt aaagatagtg ataactttga atttgaagtt aaactttcac ttaactacca
    28981 tatgggtact gataatcaag cagcgtttag agacttcttt gtcactaatg cggatgctga
    29041 tatttacttc actggcccac ttggtgcata taacgctatc aacttagcac catttgcctt
    29101 tgaaggtaca ttatccttaa cccctcctgt cgtttcttaa aataaacgca tgaagcttgg
    29161 ccccagactc ctggggcctt tttcatttgg caatttttat aaataggcgt ataattaaaa
    29221 agcactacgg accgttagct cagttggtag agcaattgac tcttaatcaa tgggtcacag
    29281 gttcaaatcc tgtacggttc accacgctaa aagaaagcgc ccgattatgt cgggcttttc
    29341 ttgtactttt ttaaaggata ggcgtataat ttgattagga acaatattta taccgaaggg
    29401 aactaaacag aatgaaatat ttaaccgtta aagaacttag cgaaaaatgg aagatgagtg
    29461 aaagacggat acgagcactc attaaagaaa aacggattga aggggtaaaa tacgaaaata
    29521 agtatttaat accagaaaat gctaaaaagc cacttgatcg ccggattaaa gggcaaagag
    29581 cgttagaaga tgcctcacta aataggcaag actttgattt taaaaactac cgcaagcact
    29641 atccgacgag tgacggctat tttggtaggt ttggtggtag cttcttacca aagcatttac
    29701 aaaaagcaat agatgaagtg tatgacggtt acttaacgat cgctaaaagc gcaaagttta
    29761 ttgccgaact acgcgaaatt agaaaaaatt atcaaggacg accaacacct gtgtatcatt
    29821 gccttaattt atcaaatcat cttggtaaag tgcaaattta tttaaagcgc gaagatttaa
    29881 atcatggtgg ttcgcataaa cttaataatg caatgggtca agccttactt gcaaaatatt
    29941 taggcaagaa aaagttaatc gcggaaactg gagcggggag tcacggcgca gcggtggcaa
    30001 tggctgcggc ttattttgat ttagagtgcg acatttatat gggtgcagtt gacactcaaa
    30061 aacaacgacc aaatgttgac cgtattaaag tgttgggcgc taatgtgatt gcggtcaaag
    30121 aaggaacaca aacacttaaa gaagcagtag acgccgcttt taatgcctac gcaaaagaat
    30181 ataaaaacgc cttttatgta ttaggtagtg ccgttgggcc gcacccctat cctttaatgg
    30241 tgcgtgactt tcaagaaatt attgggcgcg aagctaaaga acaatttgta gcaatgactg
    30301 gtgaattacc agatgtcgtc gcagcatgtg ttggcggcgg gagtaacgca attgggatgt
    30361 ttcaagcttt tttagaagaa aaagttaata ttgttggtgt tgaagcgctt ggtcaaggcc
    30421 caacgattgg gcaaaatagc gctgcggtat cacttggtaa agaaatgatt ttgcacggtt
    30481 ttaactcatt agtgctgcaa aatgaagacg gaagtgtggg agatgcttac actatcgcga
    30541 gtggtttaga ttatccaggc gttggtccag aacacgcttt tctccatgaa ataggacgcg
    30601 tgaagtacga agcaataact gatgaagaag cagcagaagc tttctttctc ttatcgcgtc
    30661 ttgaaggtat tattcccgcg attgaatcaa gtcacgcagt agcttatgct attaaatatg
    30721 cgcgcagcgt gagtgctgga agtattttag tgtgtttaag tggtcgcggt gataaagata
    30781 tggaatttat gacgcgtgag tatccaaata tgtataaata ataatttgtg acttaaatag
    30841 tgcataagaa aaagccaggt cgccctagct ttttagaatc ttttaaaatt atttagttaa
    30901 ctaaaatcgt aataaattaa taaattatca cttacattaa tgctgtattc ttcattaaca
    30961 aaccgactat cgaagtgaat ttccattagt gtacccttac gattataaat aaatgttccg
    31021 tatgaataat ctttatcgct tgtataatct ctgaattcaa ttttaaactt acgggagttc
    31081 gtaaaatatt tgtatttttg ttgttgctca actgtaaacg atttaatagt cattacatgg
    31141 tcagagacgg attttttaat ggtgtaaccg tgcggatctt catattcttc gtccccaacc
    31201 cacacgccgt cagtcagtgt atatttttgt tcaaagtcta cgccattatg ttttccggtt
    31261 aaggtaactt ttgaatgttc tggcgtttta agtttttctg taatcccgac catccgatca
    31321 aaatcaacag ggatgtacag gatcaaatta tctaatgatc ctctaaacca aaacaatacg
    31381 caacgcggat taaaaaaatg attgtttata tgaatgaaca tttccgtgat cctaatttaa
    31441 ccattgccaa aatagcggag tcttctggtt attctttaag tcactttagt aaagcattta
    31501 aactatacat ggggcattcg cccatttcgc atcttaatcg tttacggctt tcagaagcac
    31561 gctatttatt acatacaaca catttaccat ttaaagaaat tgcgctccaa tgtggcttta
    31621 aaaatatttc tacctttacg gaaagtttta aacaaagtat aggaatattg cctaaagatt
    31681 atcgcaaaaa gtatcaaaca ccaaatagtt aatagaaaat aaaaataatt aagttagcat
    31741 ttaattgatt tatagcgata aactcgtgat aaataggtgg tttttatatg tgcgttttgt
    31801 ttttataata acgcttacat ttttggggtt tattatttat aataatagag tcattaaaac
    31861 ggaggtcaaa atatggctat taagaaaaaa tttgtctccc tagatggaaa cgccgcagcc
    31921 gcagaggtaa gttatctcta cacggatatc gcggcgattt atccgattac cccatcttca
    31981 ccgatggcgg aactcgttga tttattcgca tcacaaggga aattaaacta ctttggcagc
    32041 aaagtaaaag taacagaact tcaaagtgaa gctggcgctg ctggtgcggt gcacggtgct
    32101 ttacaagccg gaaccttagc gacaacatat accgcgtcac aaggtttact tttaatggtg
    32161 ccaaatattt acaaatgggt tggtgaactt ttaccagcag tactgcatgt tagtgcccgt
    32221 agtattgccg cgcgttcctt atccattttt ggtgatcatc aagatattta tgctgtacgg
    32281 caaactggtg tgacgatgat tgcaagtcac agtgtccaag aagtcgctga tttagcgcac
    32341 gcagcacact taacagcaat tggatcaagt gctccagtct tacacttctt tgatggtttt
    32401 agaacatcac atgaaattca aaaagttgaa gtcattgatg aaaatgaatg gaagaaatta
    32461 ttaccagaag ataaagtagc ggaattccgc gctcgtgcac tgaacccaca cgcaaatcct
    32521 gtgacccgtg gtggtgcaga aaatgatgat atttatttcc aaggtaaaga agcacaaaat
    32581 acacattacg ctcgtgtgct tgatgttgct tgtgattatt ttgctaaagt tagtaaagta
    32641 accggtcgtc actatgcacc gtttgtctat ctcggagcca aagacgctac aaaagtaatc
    32701 gtcgcgatgg gcagtgttac tgaaacaatc cgtgaagtcg ttgccgattt aaataaaaaa
    32761 ggcgaaaaag ttggccttgt taaagtgcac ttatatcgtc cgtttagtgc caaacattta
    32821 gtcgatgttt taccagaaac agtgaagaaa atcgctgtct tagatcgcac taaagaagta
    32881 ggagcacttg gtgaaccact ttatcttgat gtgttaaccg cacttaaaca acaaggacgg
    32941 aaagttgatg ttatcgttgg tggtcgctac ggaattagta gccgtgacac acaaccaaaa
    33001 catattaaag gagtatttga cttcttagat aataaaccgt ggcattcatt taccgttgga
    33061 attaatgatg atgtcactaa acttagtgtc ccatttgatc acgattatca tattgataac
    33121 ggacatacgg aatgcttatt ctacggttta ggaagtgacg gcactgtgag cgctaataag
    33181 tcaagcgtcg ccattattgg tgaacacact gataaatatg cgcaagctta ctttgcctat
    33241 gactcacgta aagctggtgg tccaacccgc agtcacttac ggtttggcac aaatacgatt
    33301 cattcaactt attacgtaga aaatgctagc tttattagtg cttcacaaga ttcttatctt
    33361 tataaatttg atatgattcg ctacttaaaa gcaggcggaa cattcttact taacacaagc
    33421 ttaaaaggtg aagaattaat taatgcatta cctaacagag ttaaatacgg tttagcaatg
    33481 aaaaatgcca agatgtatgt aattgacgca aataagattg ctagtgaaat cggtatgggt
    33541 cgccatacaa acacaatctt acaatcttca ttcttctatc taaatcaaga tattatgcct
    33601 tacgaaaaag cgttgcgctt aatgaaaaaa tacgctgaag cggcttatgg tcgtcgcggg
    33661 aaacgcattg tcgaacttaa ctatgcggca atcgatgctg gctcacacgg tatcattgaa
    33721 gttaaagttg atccagaatg gaagaaactt aaaccactac gcactgtgga acttatgggt
    33781 gataagtatt ttgatgaata tgttaaccca attgataact tagaaggtta cgacttacca
    33841 gtttcaacat tcacaaagtg ggacttactt gatggcacga tgactaatga catcaccttc
    33901 aaacaaaaac gctcaatcgc gacaatggtg ccacgctggg ttaaagaaaa ctgtatccaa
    33961 tgtaatacat gtagtctagt gtgtccgcac gccacaattc gtccgttctt acttgacgaa
    34021 aaagaagtgg caggcttacc agaaaatgtg cgtgaagaaa cagtcgcggc aatgggtgga
    34081 ccaagcgtag caggcttacg cttccgtatt caagttagtc caatgaactg tgtcggctgt
    34141 gccctttgtg ttgtcacttg tccaggacgc ggcgggaata aagcacttga aatggttgaa
    34201 gcacgtagtc aatttgacat tgaacctgct gccgactact tatataagca agttacttat
    34261 aaaaccgaca aattcccact taattcacca aaaggtgttg gtttcttaat gccttatatg
    34321 gaaattagcg gagcatgtgc gggctgtggt gaaacacctt actaccgctt aattacgcaa
    34381 ttattcggta atgacatgct agtagcaaat gcgactggct gtagctcgat ttattgtggt
    34441 agtgtcccag gtacaccatt taccacgaac aaagacggtg aaggcgtcgc ttgggcgaat
    34501 agtctgttcg aagataacgc agaatacggt tttggaatgc ggatggctaa cgattataaa
    34561 cttaaattaa tcgctgacat cttcactgcg gctttaccaa aagttgaacc agaactaaaa
    34621 gaactccttg aaaaatatct cgcaaatccg cgtgaccgga aagaagttag aagctactta
    34681 ccacgcttaa ttgaattact tgaaaaaagt aaagacaaag gcgttaaaga agcacttaaa
    34741 tataaagatg acattcttga taaatcagtg tggattattg gtggagatgg ctgggcatat
    34801 gatattggtt acggcggtct tgatcatgtc cttgctagtg gcgcaaatgt taatgtgctt
    34861 gtgttagaca ctgaagtgta tagtaacacc ggcggacaaa tgagtaaagc aacgcaaact
    34921 ggtgctattg ctaaatttgc cgcaagtggt aaaacagggg caaagaaaaa cttagcaatg
    34981 atggcgatgg cttatgggga cgtttatgtc gcccaagtcg cacttggcgc taacccagtt
    35041 gccacgatcc gtgcgcttac tgaagcggaa aattacgacg gaccatcaat tgttatcgct
    35101 tatagtccgt gtactgagca cgggattaaa ggcggactta gtaaccatat cgccgttgaa
    35161 aagaaagccg tcgctagtgg ttactggcca atcttccgtt atgacccacg gcttgaagca
    35221 gcgggtaaat caccacttac ttgggatatg aaagaaccag attacacaca attccgtgaa
    35281 tttgtgatgg gtgaaacacg gtttagtcaa ctcccaactg tgaacccaga aaacgctgaa
    35341 ttcttactta gtaagagtga agaagaagcg aaaaaacgct acttccgtct taaaaagttt
    35401 gaataacgct ctttaaatta aaagagagcc tcgggacgac cccgaggctt ttcattttgg
    35461 acctcatttt ggacctcatt tttaaatatt atatacgaca atatatacga tatctgtata
    35521 atttcgatta taaagtgtaa cgataacacc attatcagaa attaaaaata ttcgtttttt
    35581 tactttgtac tctatgttta aaaatataaa taattagcta tttattatct aaaaaatatt
    35641 gaatattaat ttctaaatct tcccacggta cacaagcgcg catattttta gcaatagtgg
    35701 aaatatgagt tccggcttct ttacccgcaa cagcagcgtc ataggctgct agtatttttg
    35761 cttcaggggc agcacatgct tcataaatcc aaagtaataa ttctggacat aatagtttag
    35821 tgtacacgcg gttttttgct gaatcagtcc aagttatacc gccttcttct agtgtacgat
    35881 agagccatga atcaaagaag tgccactttt gatcaggttc acagattgat ttatcgacga
    35941 ctttaccaag ttgacgcgcg taatctatat aaccacca
//
LOCUS       DAOSTA010000006        35651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_1318_length_35651_cov_8.598073, whole genome shotgun sequence.
ACCESSION   DAOSTA010000006 DAOSTA010000000
VERSION     DAOSTA010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 35651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35651
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1318_length_35651_cov_8.598073"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            711..1442
                     /locus_tag="PLU08_01220"
     CDS             711..1442
                     /locus_tag="PLU08_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70362.1"
                     /translation="MGNENLMLQHESKHYIFNYYAGSKAAEDILLIAEHQQACYTHIC
                     KVLGVEAKFKIKYFLCNSPEEVGKIYGDNEPCNAFTSSPDSIYAVYNDDIQCIGFHED
                     AHIISYTINRPESAAIREGLAMYFDRVWWGISNEDWTLYYLKTNKYISICNLLDNKYF
                     FSEDCSLTYPIMGAFTNYLIVHYGIKKYLVFYRYKDNIFDGFEKVYKLSVNELEMIFK
                     KEIAKLELTNDTESKIKELLMKNKT"
     gene            1692..2147
                     /locus_tag="PLU08_01225"
     CDS             1692..2147
                     /locus_tag="PLU08_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70363.1"
                     /translation="MDLNLLQPKVEELLKERGYRLVSMRFLNEEGTRFLRVIVDKYQH
                     IISLDEIVAISEAIDAILDDDEESAFTLDITTTGAEKEIALQEVNDYLGLFIKLTMKD
                     GVKGPNTVKGILEAVSENEVTISHNDKGRIKKETYAQSDIKTINRAVKC"
     gene            2162..3829
                     /gene="nusA"
                     /locus_tag="PLU08_01230"
     CDS             2162..3829
                     /gene="nusA"
                     /locus_tag="PLU08_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOQ70364.1"
                     /translation="MSKKLTKAQLRKKQERFEAMREMFREVAEKHNLKEEVVLNVFMR
                     AVQNVVARQKGGDDVHVEVVINEEMFDFEARVLKRVVAHDDDIEDDALEVCLEVARTF
                     DADAEVDALVVVERSFLGDFNSKLSNNVAHNFRHQLIELEKEQLFTLFKDKIGELVKG
                     TVEKNERITYINLLGITSVFFDERGRHAIPGEVFKPGQELNFYIVDVVSGAKGANIII
                     SRSHPNFVKRLMEREIHEIYDGTIVIHDIVREEGERVKVAVESLDPNVDPIGACIGPN
                     ASRIQKITAELGPDGRKEKIDIIPWVKNEALFIIEAFRPAVPLGLKIDEENKTAQVVF
                     SEEEKGKAFGHKGANIRLASKLTGYDLTILDLNEASEQGVEFLTKADIEYEERLAEQE
                     RTRERILRELARAEEVAQRAASQKSLLEEEVNSLNIDDLEYEDAFKDEEKVVETPKVV
                     TPEIKEEKKEPVVQKVKTTISLADLEAELAKEKEEIEQAQQKREERRSRTKQEVKQPE
                     VVEPTLPTGPKMDIYTEEELAELDAELEEVYEDDDLDFEDFDEYYDN"
     gene            3840..4097
                     /locus_tag="PLU08_01235"
     CDS             3840..4097
                     /locus_tag="PLU08_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOQ70365.1"
                     /translation="MKKIIVRRCIVTHESHEKRDLIRVVRTPDGRVVIDNTGKVNGRG
                     AYLVRNKEVILLAQKKNALSQALNIEVDQVIYEKLLEEVND"
     gene            4090..4392
                     /locus_tag="PLU08_01240"
     CDS             4090..4392
                     /locus_tag="PLU08_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70366.1"
                     /translation="MTNELNNFLHLLKRSGHYRFGADYLRESNARKIALVLTSDDLSE
                     YTKTKVNNFTLAHNIAQFDVPANIMSMLYPGKTIKVLVVTNHQSAQKIANLMKEGN"
     gene            4394..6220
                     /gene="infB"
                     /locus_tag="PLU08_01245"
     CDS             4394..6220
                     /gene="infB"
                     /locus_tag="PLU08_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006506691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOQ70367.1"
                     /translation="MSNEKKRISNVAHHSKKQSGPCVEGRKLIYQGPIKVSDLARLLK
                     LSEAQIIKNLFMKGQMVTINNVLSPETVAELCLDFNFDYDFKEETEDDCDFGLSHIVD
                     DPKDLKPRPPVVTIMGHVDHGKTTLIDTIRSSSIVSGEAGSITQAIGAYQKEFHGKKI
                     TFIDTPGHEAFTAMRSRGTKVTDIVILVVAADDGVMPQTIEAIDHARAAEVPIIVAIN
                     KMDKPGVDPEKVKNELLAQNVIAEEYGGDVIFQPISAKKGTGIDELLDAILVTAEMLE
                     LEANPKRYAYGTVLEARLERGEGPKAMLLIQNGTLTNRDFVVAGAAYGKVRRMTNEHN
                     KALKSAGPATPVAIIGLDEVPEAGDNFIAFETEKEAKELATKRKQIRIEESRGSSEGM
                     KLSDLHKQIAEGETVVVNVIIKADNSGSAEAVRGSLEKINVEGVRLNIIRASAGQITE
                     SDVLLASASNAVIFGFNIRPNNQVRDLAERLKIEIHLHRVIYALLEQTEQALKGLLKP
                     EFEEKVTGQAEVRAIWHASKVGTIAGCYTLSGVIRRNQTVRLLRDGVIIYEGEVATLK
                     RVKDDIKETRAGYECGMTLLNYNDIKEGDIIEGYAMVEVKDA"
     gene            6213..6554
                     /gene="rbfA"
                     /locus_tag="PLU08_01250"
     CDS             6213..6554
                     /gene="rbfA"
                     /locus_tag="PLU08_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOQ70368.1"
                     /translation="MPKSRNLSRNESLMLRHLSEIISYKVGDPSLGLATVSAVELTRD
                     YSYAKVYISFLGDGDVEAKMEILNNASGFIRHELSQKMNIRKTPELRFIYDDTFERVA
                     KLDELLKRTKK"
     gene            complement(6631..7827)
                     /locus_tag="PLU08_01255"
     CDS             complement(6631..7827)
                     /locus_tag="PLU08_01255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ70369.1"
                     /translation="MKHFRKLLAKIKKADEKFNLIQSGDKIVIGISGGKDSIVLFEAL
                     RTYKRYSKKDFTLYPVILDLGFSGFDASSYTAFFKEREYDLIVNDSRDVSKILSVQKE
                     KQGLSKLPCSICSRMKKAAINEVANKLGANKVAFAHHIDDALETLFMNMIGGGRFATF
                     SPQMYLERAEITFIRPLILVDEKLIIRVQKDLKLPLLPYNCPNENKTRRDDIKSLLNA
                     IYKKYPEAKTNFELMLINDKEFDLWYKDYATLINDDCVIVKVTTPAHFNDALYIRYKV
                     FVEEQNIKVEDEYDTNEKDYTSLVLYVKGNKAATIRYKYDALARRAYLGRIAVLKEYR
                     GLKLGMTLITYVEQKLSVKYRPLTFHIGGQSHLLKYYESLGYKRVGESYMDAGILHYH
                     FEKKVI"
     gene            7870..8727
                     /gene="truB"
                     /locus_tag="PLU08_01260"
     CDS             7870..8727
                     /gene="truB"
                     /locus_tag="PLU08_01260"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOQ70370.1"
                     /translation="MNKGKNGILLVNKEPGYTSRKVGNMLSRKLKIKKVGHLGTLDPF
                     ASGLLIFAVGEGTKVLPYLEDEVKTYVATLYFGLLTTTLDPDGEVIKKEKVPPFSKAE
                     IEKVIKQFTGEVTQIPPTTSAIKIDGKRAYEYARKNIEVDMPSRVVEVYTIEVLEYNH
                     PLLVIEATVSKGTYIRTLAQDIALALGTNATTIKLVRTRHGDFSLEKAKLFEEVSEED
                     IIDPSLGLKKFPIYKLSDEEVKRVRNGMTIKLNASEEIVQIHNNSGVLQALYKKKGDN
                     YIALRGFNL"
     gene            8724..9644
                     /gene="ribF"
                     /locus_tag="PLU08_01265"
     CDS             8724..9644
                     /gene="ribF"
                     /locus_tag="PLU08_01265"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOQ70371.1"
                     /translation="MIAKHLDLSRITSLAPSTALVVGTFDGVHIGHKYLVEEGFKAAD
                     NVAVLLVYTNKKLTKLHQKSGVLTSLEDRVEIFEKFGVKTVYLLNLGDELLKLEPCEF
                     INKVLVPLAPNSVIVGTDFRFGRGAKGTGEDLREFGKNVFTTRVLAPLYYENEKVSTS
                     LIKELLSKGKPKIAKSLLGRYYALTGLVTKGYGLGNKLGFPTANIALDPSYFVPAHGV
                     YFVRVKFRANYYFGMASLGYHPTVNKVNVPLLEVNIFNFNENIYHDALTVEFIDYLRP
                     EFKFESVDTLIKQLQKDRENCEFKIEKGEY"
     gene            9645..10859
                     /locus_tag="PLU08_01270"
     CDS             9645..10859
                     /locus_tag="PLU08_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOQ70372.1"
                     /translation="MQVFNVKNNVYYIGVSDRGLRVFDIMMETKHGTTYNSYLIKEEG
                     GVTIIDGVKAGFTDKWLDNIRSLVDIKDIKYLVVQHTEPDHSGAIPALLALNPNIEVY
                     GSFMAMANLKEINNAPFISNVIKPKQELKLSERQSLFFYPVPNVHWPDTIITHDPVNA
                     IAFTCDFLGQHIAPQQLLYSKEPNKKIIEEEIKLYYDAIMAPFKSDVLKSVATLKSLD
                     VSMVLNSHGYILDTKEAVDWAINNYIKWSTVNKSEKLTVTVVFASAYGYTRSMSEAVI
                     SELEKASALVHVFEVPRDDLATIVARIEESHALLVASPTFVGDGIKPVYDVLNSLNPV
                     MCFGKYFSAFGSYGWSGEAVRNYMNRASELRFKKVDEGFKVRLKPSEKELEDTRLYAR
                     NFIMKIKAELNK"
     gene            10992..11261
                     /gene="rpsO"
                     /locus_tag="PLU08_01275"
     CDS             10992..11261
                     /gene="rpsO"
                     /locus_tag="PLU08_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOQ70373.1"
                     /translation="MALTKERIAEIVKEFGANEKDTGSTPVQVALLTEQIRELTEHMK
                     VHKHDYHSQRGLLKLVGQRRGLLDYYNRVNREGYLALIAKLGLRR"
     gene            11322..13484
                     /gene="pnp"
                     /locus_tag="PLU08_01280"
     CDS             11322..13484
                     /gene="pnp"
                     /locus_tag="PLU08_01280"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOQ70374.1"
                     /translation="MKKVYEMMFHGRKIVVETGEFAKQADGAVLVRYGDTAVLSTAVA
                     GNNPTNLDYFPLSVAYQEKMYAAGKIPGSFLRREGRETEHATLIGRLIDRPIRPLFAE
                     GFRNEVQVINTVLSADYDCTPEMTAMFGSSLALTISDIPFEGPIAGVQVGRVDGKFII
                     NPTTEENAKSDLQLTVAGTKRAINMVEAGANEVSEEDMLEALMLGHEAIKELCAFQEE
                     IQRDIGVAKREFPLFVPDESLVAELKEKYTGKLTEAISVFDKLERSAAIEAVKGEMVE
                     EYEAKEYESDDDKRRVISDVKALHEVMVSDEIRRLITHEKVRPDGRKVDEIRPLDSQV
                     DLLPRTHGSALFTRGQTQVLSVVTLGPKSDEQIIDNLTDEESRRFMHHYNFPPYSVGE
                     IKRLGGPARREIGHGALGERALLAVIPSDEEFPYTIRVVSEVLESNGSSSQASICASS
                     MALMAAGVPIKDMVTGIAMGLITSGPLDGNHPYTILTDIQGLEDHFGDMDFKVAGTSK
                     GITALQMDIKVRGISKEVLAESLAQAKKAREEIRANQLSAISAPREDVAEFAPKMGKM
                     TIDVDKIREVIGTGGKVINKIIEDNDGAKIDIDDDGLVVVYHMNRATINNVLATIKAI
                     VKEAKAGEIYEGKVTRVEPYGVFVNLFGNVDALVHVSDLDWAYVDDASKLYKIGDTLK
                     VKVTGVDEKGKVKASVKVFKAKPEGYTERKSGGRRNNK"
     gene            13643..15073
                     /locus_tag="PLU08_01285"
     CDS             13643..15073
                     /locus_tag="PLU08_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOQ70375.1"
                     /translation="MYDIIIIGAGVIGALIGREASKYELNILMLEKDNDVGNGTSAAN
                     SAIVHSGYDPKPGTLKAKFNVASVPLYKDLCSDLDVEFKRIGSLVVAQGEEELPRLDE
                     LKARAELNGVEVKMLNKAEVHALEPNLHDHIVGGLFAPTAGIVNPFELTAHAIENAIE
                     NGMTLKLNEEVVAIKKLEDGFKVTTKNGDYFSKVVINAAGVFADDIINMVSKADFEIT
                     PRKGSYFVLDKLTVPLVNTVLFPLPNIHSKGILVVPTTAGNTLIGPTSEQIMSKVDVG
                     ADYASLEFIRKNANRLLKDIPFHKTIRTFSGLRATPSTGDFIIEHDKYVKGLINVAGI
                     ESPGLASAPAISLYVVNELVKDLLPLKVKANFNPRVRPLIRLQRLSTKEIQAKVKENK
                     DFATLVCNCEKVTKAEIIDVLSRALPVNSVKAMKKRLRTGFGLCQGGFCTAKVIAIMS
                     EYYGIPVDEIAYNEKESYVVKHGKKV"
     gene            15057..16310
                     /locus_tag="PLU08_01290"
     CDS             15057..16310
                     /locus_tag="PLU08_01290"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ70376.1"
                     /translation="MAKKYDLIIVGGGSAGLGAAVAAYDAGIHNILLIEKEATFGGIL
                     NQCVHDGFGVHLFKKTLTGPEYAHYLVEMLKERPFDILLSSNVTDISDKQEVTVFVAG
                     QGVMLFKTKAIILTTGSYERSAGAINLPGDRGAGIMNAGQAQYFLNLKGYEFAKDVLI
                     VGSGDIGLIMARRLSLLGIKVHAVSEIMPHSSGLKRNIVQCLDDFNIPLYLSHKVTDV
                     RGKPRIEEVDIAELDDKFEIIPGTTKTFKVDTLLLAVGLIPYVALSDNLGIPTSKSRG
                     PVVNNNYQTQLPWFFVAGNALHIHDLADDAYFEGEEAGRAASLFINGKLKAPTNTLQV
                     VPGYGINYVIPNNLELPLNTEELVLKFRPKENYENKRIVVKIDDTVIFKKFFPFLFPS
                     ELVILKVKKELLVAGEKLIVEVSDE"
     gene            16303..16650
                     /locus_tag="PLU08_01295"
     CDS             16303..16650
                     /locus_tag="PLU08_01295"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOQ70377.1"
                     /translation="MSSDKKVTCLVCPRGCIMTVSSDLKVSGNFCARGVPYALQEVKE
                     PKRNLTYVVQVEGREAPLPVRTDSMIAKHLIPDVVKLLRTVTIKPPVAFNAIIIANVF
                     DTGVNIISSAEIK"
     gene            16733..18421
                     /locus_tag="PLU08_01300"
     CDS             16733..18421
                     /locus_tag="PLU08_01300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOQ70378.1"
                     /translation="MNDEVKIFALGGLDEHGKNMYVIEINNDIFVLEAGLKYPDKSSP
                     GIDFIIPNHEYLITNKSRIKTYFISHGHDDQFGALPFIYKQAPAPIYTTKAAAMMLER
                     FTRRVGVKDVKYEIFTIEPSSQHNIAGHKFTFFQTTHSAMHSFGIALDTSQGTVVYSG
                     DFIIEYTNSARYKLDLNSIAKIAEKNVLCLLSESSGAEKAGYTSPTHRLAPNIVPIFR
                     ETKGRIFISLYDQSTYNIEELVQVVINAKRKIAFYDPNTEEYFRDFAKAGLSLIDERY
                     IISNDDLLRARDQDIVILMLGAGEKIYEKIIALSNRENPDKKIVLGKDDTFIMALPAP
                     ATLEVIATEALDSLYTTGCNVVNITRKLFTNMHAQEEDLRMLLSLLKPKYYIPVKGLY
                     RQQIANAQLALHSGLKFSHHNIFLLDNGVSVEFNDGVGRVKMSPLDLIPSGDVLVDGT
                     GIGDINRGVIEDRQRLSEDGIIIMAIAVNYDERTITAGPDVQMRGFVYVKDSEQLLKQ
                     IRQIFINTVSEALDARQFDIDGAVKETESRVEKYIFRETRRHPMILPLIRVINE"
     gene            18497..21085
                     /locus_tag="PLU08_01305"
     CDS             18497..21085
                     /locus_tag="PLU08_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOQ70379.1"
                     /translation="MSEAKTNLLMQQQRLSRGLKGIIVALLALIGILKISIGGVINVP
                     VALLFGDLFVVFFALQVLFAFYLIFFPKAKSKKRVTLVFLSIFGLLIISSAFYAGNLR
                     NYMRDGTTFKDNLDVMVTLARDTFSFTPPSNAYFYNYGGILGEGLLFVLKDLPVWLII
                     IILVLANIGFATMLLWPLLVKIYAAIKEEIRDLKKRRRVLHEAGQLHKEEAPTEEDQV
                     PVTEIMNDNEVWGSNLLHRVGPSASEAEEKLGPPPALNVVKNENSGMHRAMPSEGFSA
                     FDEHFELPTLDTNEEEFEALDPTVIRTMEKVESEEDFQELERAVDEKGSFEAVSFTPH
                     HEEPTNTLNAEPKKDENVLREALEERYEPEPLPPLPEPVVKQENEQKPRISSNFANFR
                     LPKLDLLDDAIDNGEEAQNREFAAEYEAKINAFFTDFNVDARVFSWTIGSTFTRFEIQ
                     LGPTESVKAINPLLNDISIRLNGASVRFEEVLPGKATSGLEVANKFRTNVNFKDCMHK
                     IVANKRKKYLIPLGKDVAGNVILAPFTDLPHLLVAGATGSGKSVFIHTVLTSLIMMHS
                     PLELRLILIDPKRVELSAYNNIPHLITPIIKEPSEAKVALDRLIDEMENRYKLFEKTG
                     VGKLSDYNEIIEEMGGEKLPVIVAIVDEFADLFESEKEISNLVLRLVQKSRAAGIHLL
                     IATQRPSVQVITGNIKANIPSRVAFMTASVTDSMTILGCGGAENLLGYGDMLVDSITT
                     NYRGLLRVQAPYVQVREVLRVTNYLRNNYMTDFYPEFLDLKEKVNVYGGGGPVISDDL
                     YQAVLDFTLTQDTISISRLQQNFNLGWPRARQLYDQLMQNGIIEPPDEANSAKGAVVI
                     ANKRGN"
     gene            21088..21408
                     /gene="acpS"
                     /locus_tag="PLU08_01310"
     CDS             21088..21408
                     /gene="acpS"
                     /locus_tag="PLU08_01310"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOQ70380.1"
                     /translation="MSIGIDIVYIPRFAGKEKLATRILSKNELEIYHMRPNKEEFLAG
                     RFAAKEAFLKAMKEGIGRVAFHKIDVVYKKDGRPEIHHDGNVYAVSISHDGDYAVAVV
                     QIKE"
     gene            21414..23585
                     /gene="pulA"
                     /locus_tag="PLU08_01315"
     CDS             21414..23585
                     /gene="pulA"
                     /locus_tag="PLU08_01315"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HOQ70381.1"
                     /translation="MAFGQFSAHLIAPRVIRVVLYSDKYQYEQTRVTIYDDNDKLVPF
                     SSKSKVVQYKVTAFELTLEQPFVLGQNYKVYLQNFGQVPLNVSEATTFPNFDTDYYYT
                     GPLGAEYTKEQTTFRLWAPLASRVILKYEVPGEKHFLYRKLKRGERGVYEYTLEGDHE
                     GIRYTYLVLNNGLEEEVTDPYALSSGPNGAFSYVINQNKYDDMNMHDDKLPPFTKPTE
                     AIIYETSVRDQTIDERTDIEYPATFYGMIEEGRKVKDTYPAGFDYLKDLGFTHLQLMP
                     VYDFKTVDETRKWQSYNWGYDPQQYFVLEGSYARDPLHPYNRISDFKLMVSRYHEVGI
                     RIIMDVVFNHVYDHETSVFEKIVPNYYFRKTSEGVISNGSGCGNDLATERPMVRRLVV
                     DACRYYAEKLGVDGFRFDLMGLIDIKTMEEVTKAVRQVKPDFLILGEGWEMMTPHPKE
                     KLAHHGNASKTPEIGFFNDAFREIVKGGSFADNLHERGYMLGATNYRLGFYYAYAGSV
                     LNTTFTPRYLNAGQSINYVECHDNGVLVDKMTVSNASECKETHLARLLSMNTIVMLSF
                     GVPFFHRGQEIGVSKYGDLNSYKSSDKVNQFPYTLALKRKEMVEYFKALTALRKDCKF
                     LHETDPKVIKNIIEFKDLPGGASQIVYNPDMDLSPYSHFNVYVNPTSEPIFISLEKPL
                     KTILSSGGYIAARSKEYFERTMVAPYSVIVLGLRKGDSGPK"
     gene            23605..23868
                     /locus_tag="PLU08_01320"
     CDS             23605..23868
                     /locus_tag="PLU08_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70382.1"
                     /translation="MLTSVFIVGTLGKNENDYRYLLVEKVPGLDYEDDEERAKYDYFK
                     VKHWSNTPSAFNRLAEGRKVALKGRLEEIEGETYIIAELYREF"
     gene            23878..24675
                     /locus_tag="PLU08_01325"
     CDS             23878..24675
                     /locus_tag="PLU08_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOQ70383.1"
                     /translation="MIKYIIWIVKVAIPLMWGYFTWMRPYARRPDKYPPELRMAKGQK
                     LARRVLRILKVDVEIRNMPDFKRDEIYYIVSNHTSVLDLVVLLAYLPIPVSFLSKVEN
                     KKIPFVRTLMRVYKGLYLERNNLRQEIKVMQMTRDSLANKEVSWLIFPEGTRSKDYHG
                     NLSDYKAGAFKAPLATNTTIVPVVMWGNQMVLPLRTRARRYKVIVEFLPLLKPEGTTL
                     ELANKIYEASNATASKLREEYGSVQKINKYGRKIMTLQLSENIKTSK"
     gene            24787..26094
                     /locus_tag="PLU08_01330"
     CDS             24787..26094
                     /locus_tag="PLU08_01330"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HOQ70384.1"
                     /translation="MRMYDIILKKRDGFALTEEEIKFFIKGVTDGTIPDYQTSSLLMA
                     IVWRGMNKEETAALTLAMMHSGDVIDLSAIKGVKVDKHSTGGVGDKTSLVLGPLVASF
                     GVKVAKMSGRGLGHTGGTLDKLESIPGTRIDIPNDDFIRQVNEVGCAIIGQTGHLVPA
                     DKKLYALRDVTATVEAIPLIASSIMSKKLASGSDTILLDVKFGSGAFMKTLKDAQELA
                     RAMVDIGKSLNRDTRAILTDMDQPLGLAVGNALEVKEAIATLKGEGPRDLSELCIEAA
                     CIMLEQAGVVKKAQDARKEVENKIKSGAALQKLVDMVKAQGGDESYITNPEKFAISQN
                     VFEVLATSEGYVEHIDSMAIGISSMKLGGGRETYEDEIDMSAGIVLNKKVGDKVKVGE
                     RLALVYTNKADEVSTPILVDIHHAFKLSDKHIDVPPIIHDYIK"
     gene            26094..26537
                     /gene="dtd"
                     /locus_tag="PLU08_01335"
     CDS             26094..26537
                     /gene="dtd"
                     /locus_tag="PLU08_01335"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012574408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOQ70385.1"
                     /translation="MRVVIQIVKDAKLEIDNKVYSSIDYGMLLLVSFTLSDTKEVAKK
                     MAEKIALMRIFPDENGKTNLNINQVNGEVLSVSQFTLYGEFNSRRPSFTKVLSGEESS
                     QLYDYFNSELRKMLKVKTGVFGSDMNITFTNVGPVTYVVESDEKR"
     gene            26524..27672
                     /gene="mnmA"
                     /locus_tag="PLU08_01340"
     CDS             26524..27672
                     /gene="mnmA"
                     /locus_tag="PLU08_01340"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOQ70386.1"
                     /translation="MKKDKANIKVMVGLSGGVDSAVALALLKKEGYDVEAAFMRNWDS
                     LLNNDVSGNPTLYDPTCPQEVDYNDAKAVAETLGVKLHRVDFVKEYWDNVFSYFLSEY
                     EVGRTPNPDIFCNKYIKFDAFLAWALEMGADYIATGHYAKLEEVNGELVMKKSADKNK
                     DQTYFLSQLSQAQLKRTLFPLGDIPKSKVREIAEKLDLSPAKKKDSTGICFIGERHFR
                     DFLKNYIPAQDGVIVDINTGKEIGTHTGVYYYTLGQRRGLGIGGLKDYPENKRWYVCK
                     KDVVNNILYVANDDEDEHLKSDKITLTDVNFINEELKSGTPLNVKLRYRQKDLPATYY
                     KNKDGAYLLFPKPYLAVTPGQAAVFYIDEVCLGGAIIGDLFYKGKKIN"
     gene            27902..30496
                     /gene="alaS"
                     /locus_tag="PLU08_01345"
     CDS             27902..30496
                     /gene="alaS"
                     /locus_tag="PLU08_01345"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOQ70387.1"
                     /translation="MKKLTSNDIRKMWLSFFKSKNHAVVEGASLIPVNDKTLLWINSG
                     VAALKQYFDGSKVSADKRLTNVQKSIRTNDIENVGKTNRHHTFFEMLGNFSIGDYFRT
                     EAITWGFEFLTSKDYLALPLEKLYFTYHPSDTETRDLWISLGVPKSHVIANEDNYWEI
                     GEGPCGPNTEIFFDLGEKIDARGPELLASDIDNDRFLEIWNIVFSQYNAKAGVKRENY
                     EELPSKNIDTGAGLERIASVLQEVPSNFETDLFMPIIKELEKLSGVKYSDDIVSFRVI
                     ADHIRTSTFAIADGASFSNEGRGYVLRRVLRRAVRYGQKLAMKEPFLYKLVQSVVDVM
                     KDFYPYLESKIDHVEKLIKAEEVRFLNTLNHGEELLRGALSNSKNISGEVAFKLYDTF
                     GFPIDLTLEIASENGASVDVKGFEVLLEEQRERARAARKNLNSMASQSEDLLKFDQKS
                     KFEEQKFSVTATVIGLFKDGVKVNESAEDVSAIFDVTPFYSESGGQVSDSGFVSNKEL
                     RAVVSAMSKAPHGQHLHHLTLDYGVIKVGDKVQLEIDGVARRSIMLNHSATHLLHRAL
                     NDVLGSEVSQAGSYVGPDYLRFDFNYNTRVTDEELEKIELIVNKYISDSVPLTVKYMT
                     LAEAQKLGAKALFMEKYGDLVRVVTFAGISVELCGGTHIDNTSKIGTFVIVSESSIAS
                     GVRRIVALTGLAAYEHLKTYSDTVKRISKELGLNDKKDVVPHVRTLVEKNALLNSEVT
                     MLKTAELHRTASALTPKKHGDTNIYLEYFTTSNREDLGVIADKVRSTDECALLVLVGN
                     NTNDFPLLISVSKELSKSLNANKVMRALTSAFGGRGGGRPDFANGVLLNLPEKTKALA
                     TILKVLDE"
     gene            30489..30923
                     /gene="ruvX"
                     /locus_tag="PLU08_01350"
     CDS             30489..30923
                     /gene="ruvX"
                     /locus_tag="PLU08_01350"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOQ70388.1"
                     /translation="MSKILGLDLGTKTLGIAISNVDATFAVAYENYVFPPHYYKKARA
                     HVLAICAKENISEIALGYPLNTDGTVGERAKSAERFRDDLLREGSPPIVLVNETYTTL
                     AATEELERLGYKKEQIKAQIDAVAAKYILETYLERKRSNERK"
     gene            30910..31158
                     /locus_tag="PLU08_01355"
     CDS             30910..31158
                     /locus_tag="PLU08_01355"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOQ70389.1"
                     /translation="MKENDVIVITTEEGGEEEFIILFTYENEERGTKYVFFYDENNDE
                     EVMCAKYFDDGTLEYVEDEEELAEMEEVFRAYESELEE"
     gene            31238..31717
                     /gene="greA"
                     /locus_tag="PLU08_01360"
     CDS             31238..31717
                     /gene="greA"
                     /locus_tag="PLU08_01360"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOQ70390.1"
                     /translation="MTNKYTLTQEGVDALEEELSVLVNVERPLVLKEIEEARSFGDLS
                     ENADYDAARNKQANIESRIMEIEHILAHKEIVEYSKTSKTVAVGNYVEVHDRTNDDTF
                     SLRILGQFEGKPLNGTVSVDAPIAQAILGKKVGDRVNVKAQPPYEVEILNISTEPIE"
     gene            31780..32316
                     /locus_tag="PLU08_01365"
     CDS             31780..32316
                     /locus_tag="PLU08_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HOQ70391.1"
                     /translation="MLKLMIGVVIATVIVIFAFSVINSNMSANPQGQSSMHVIDDNFL
                     SITVTGQVVKPGTYVMKKDETIGDLIMAAGGPTSNADARSYIEDTVLIAGVSYYIAPI
                     NDLDDYCGEMPLHKVNINADPKDKLMEVNGIGETLAGAIIQYRGEHGEFTYLEELMKV
                     KGIAKATFEKIKNHVTLR"
     gene            32313..34310
                     /locus_tag="PLU08_01370"
     CDS             32313..34310
                     /locus_tag="PLU08_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOQ70392.1"
                     /translation="MNLLFIFLSFCCGLLAFRLPLFILPFLLLLVRTFKNKLTFTFVI
                     VFFALGLIIMIISNFISKLPFESSFGLITKAGTNHIIVLTVRGKFYVRLENNKLVEGD
                     IILLNGYKVAYFFKRLEGEFNYAKYLENKGIKSALNLSFYELKVPSLLRLFNKFTVKK
                     NTYPPHLEHIKNLLLIKTINYDDPFIHELIRLDLLFLISLTGAHLSFLRRVVKKISSV
                     FVNEFLQEVISYLCLIPLFLLNITKFAFYRIFLTGILRLINKKHLNAYFNSVELTCIA
                     AFFFLVINPYFVFDPAFYLAYLLTFLFQMVRPSRSVKGRLQSSLIVMCVIVPYNIFTS
                     GTFNLVYIFISLLLVPLIAVWTLVYFVTLFIAPLQNVGINTLNLIYHLVVLMKKINIT
                     LYFGDLNVLSKLLLVTFISVLLYAFNAKIKPLQKDITLLITTLLLLNLLPLNHYFEYS
                     ISFINVGQGDSTLLRIKNKYVLIDTGGLTYKDVGNDILIPYLKRNKIYRLDYLIITHD
                     DFDHCGAKEALMHNFTVKNVITERQYFPLNVNGITLENLNNKYYDDVNLDSLIIYVPF
                     PKLKILLMADAGIPNEVDLIKTYPTLEVDVLKVGHHGSNTSTSDDFIRHYKPQVAIIS
                     CGYQNKYGHPHHTVLSTLQKYDVKIKRTDTLGTITLKTCII"
     gene            34313..35263
                     /gene="holA"
                     /locus_tag="PLU08_01375"
     CDS             34313..35263
                     /gene="holA"
                     /locus_tag="PLU08_01375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOQ70393.1"
                     /translation="MLYLFAANEYALVEKVIRKTVDALLPERNAFNYVRYDMRETPFS
                     EIIEDALSYAFDSSVRVIVIDHASVLTTSNEKSINKDIPDEQIDLLSRLSEDTHLIFI
                     ARSSQINPKHKIIKFIENNGKITVEKDIDKNDWQRFVRAYFAKENLPIDDEAASELLS
                     RVTNLGSFLQEAEKLAIYGKRVTLPLVEELVTPLLEENSFALVNAFISGDKAKTIKIY
                     QDFKVQNQEPVAFIAQVGNQFRTYAQIYICHEEGMTNEEIASFLKIHPYHVKLAMDQR
                     RRTSLEEVFNILLALADLDYKIKSGQIDRFYGFEMFLLNY"
     gene            complement(35260..35526)
                     /gene="rpsT"
                     /locus_tag="PLU08_01380"
     CDS             complement(35260..35526)
                     /gene="rpsT"
                     /locus_tag="PLU08_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOQ70394.1"
                     /translation="MPNIKQQIKRVKTNEKARQRNAHFKSMVRTQLKKVRVAIDAKDV
                     ALAEAEYKKAVSLLDKAAGRGVFHRNKVARHKSELARQINELSA"
BASE COUNT        11726 a         5726 c         7426 g        10773 t
ORIGIN      
        1 ataacagtat caagtttaga aatggattta ctataaaagt taaaattagt ggattatatg
       61 taaataatag tgacagatag tgattttatt ggtattcatt gtgttccaga tggtgctgta
      121 ttttgatata caaattgttg gtaatcggaa taataattat ctaaataaaa tagttagtgt
      181 aaaacttact taattgggct ttggtcgcca cagagtcgat atcactaacg aattattaag
      241 ttagattatt tgttatttca agcaaaataa aaaggcacag atgagtcgaa acttaattcg
      301 cgcattacat cgtatgccca atattaattt acctattttt ttcatttaag tcaattcaat
      361 taccaattaa tgacaaatta aatgactaat taattttaat ttactataag ttattaataa
      421 ttggaaagtg ttagaattta aaaaatgccc ttgaacagtc caagggctct ggagtgatta
      481 gctcccaata agcgtaatca gcggctatca ccagcgttat tttccttaat ttcgcaaatt
      541 aagtcaagta gtgatgttat ttaataaagt ttaaataata ctattagtta agagattgtt
      601 aactaaataa gagtaagaag cgtttatcgt agtataatta gctaagaatt aaagcactaa
      661 cttatgagca ataagatgag gcgaaaggac gccttaaaag tttacgacaa atgggaaacg
      721 aaaacttaat gttacaacat gaaagcaaac actatatctt taattactat gcgggtagta
      781 aagctgcaga agatatcctt ttaatcgcgg agcatcaaca agcttgctac acacatattt
      841 gtaaagtttt aggggttgag gctaagttta aaattaaata ctttttatgt aattcgcctg
      901 aagaagttgg gaagatatat ggtgacaatg aaccgtgtaa cgcttttact agttcgcctg
      961 actcaattta tgcggtatat aatgacgata ttcagtgtat tggttttcat gaagatgctc
     1021 atattattag ttacacaata aatcgtcccg aaagcgcggc aattcgtgaa ggactagcaa
     1081 tgtattttga ccgtgtatgg tgggggataa gtaatgaaga ctggacacta tactacttaa
     1141 aaacaaataa atatatttca atttgtaatt tacttgataa taagtacttt tttagtgaag
     1201 attgttcatt aacttatcca attatgggtg cttttactaa ttatcttata gtgcactacg
     1261 gaattaaaaa atatttagtc ttttacaggt ataaagataa tatttttgat ggttttgaaa
     1321 aagtatataa attaagtgta aatgaattag aaatgatatt taaaaaagaa attgctaaac
     1381 ttgaactaac aaatgatact gaaagtaaaa ttaaagaatt attaatgaaa aacaaaacat
     1441 gaaaatcaaa acagtactgg ttaagtaagt cttttttact attgcgacgg tattggatcg
     1501 tgtacttctt aagaaaaaat cattgtaaat ttaaggtgta aatatgaaat aatcgctaat
     1561 attatagtaa tttgacactt tttagttgca aagttgtaca aaatctacta ttatgatact
     1621 gtaagtttta cttacaaatt aattcaaact ctagagagtg ggtacgccca ctctttgttt
     1681 acaggaggat tatggattta aacttattac aaccaaaagt cgaagaacta ttaaaggaac
     1741 gcggttatcg cttagtaagt atgcgtttct tgaatgaaga aggtacacgt ttcttacgcg
     1801 taatcgttga taagtaccag cacattatta gtcttgatga aattgtcgcc atcagtgaag
     1861 caattgacgc aatactagat gatgatgaag aaagtgcctt cacccttgat attacaacga
     1921 cgggcgcgga aaaagaaata gcgctacaag aagtaaatga ttatctaggt ttattcatta
     1981 aattaacgat gaaagatggt gtcaaaggtc ctaacacagt aaaaggtatc ctagaagctg
     2041 ttagtgaaaa tgaagtgacg attagtcata acgataaagg acgaattaaa aaggaaactt
     2101 atgcgcaaag tgatattaaa acaattaatc gcgcagtgaa atgttaaaga ggaatacgaa
     2161 aatgagtaaa aaattaacaa aagcacaatt aagaaaaaag caagaacgtt tcgaagcaat
     2221 gcgtgaaatg tttcgtgaag ttgcggaaaa gcacaactta aaggaagaag ttgttcttaa
     2281 tgtttttatg cgtgctgtcc aaaatgttgt cgctcgccaa aagggtggag atgatgtgca
     2341 tgttgaagtc gtgatcaacg aagaaatgtt tgattttgaa gcacgggtct taaaaagagt
     2401 tgtcgctcat gatgacgata ttgaagatga tgcacttgaa gtatgtttag aagtagcgcg
     2461 tacatttgac gctgatgcag aagtcgatgc attagtcgtc gttgaacgct cattcttagg
     2521 cgatttcaat tcaaaattat caaataatgt cgcccataac ttccgtcatc aattaattga
     2581 acttgaaaaa gaacaactct tcactttatt taaagataaa attggtgaac ttgttaaagg
     2641 taccgtggag aaaaatgaac gaattactta tattaattta ttaggaatta caagtgtgtt
     2701 ctttgatgag agaggacggc acgcgattcc tggtgaagtt tttaaacctg gccaagaatt
     2761 aaacttctat attgttgacg tcgttagtgg cgctaaaggg gcaaatatta ttatttcccg
     2821 cagccatcct aacttcgtaa aaaggctcat ggaacgcgaa attcatgaaa tttatgatgg
     2881 tactatcgtt attcatgata tcgtgcggga agaaggtgaa cgcgtcaaag ttgcagttga
     2941 aagtcttgat ccaaatgttg atccaattgg cgcctgtatt ggtccaaatg caagccgcat
     3001 tcaaaaaatt actgctgaac ttggtcctga tggccgtaaa gaaaagattg atattatccc
     3061 atgggttaaa aacgaagcgc tcttcattat tgaagccttc cgtccggctg tgccgcttgg
     3121 cttaaaaatt gacgaagaaa ataaaacagc acaagtcgtc tttagcgaag aagaaaaagg
     3181 taaagccttc ggtcataaag gcgcgaatat tcgtttagcg agtaaattaa ctggctacga
     3241 cctaacgatt cttgatttga atgaagctag tgaacaaggt gttgaattct taacaaaagc
     3301 ggatattgaa tacgaagaac ggctcgctga acaagaacgg acacgcgaac gtattttacg
     3361 cgaacttgcg cgtgccgaag aagttgcaca acgcgccgct agtcaaaaga gtttgcttga
     3421 agaggaagtt aattccctta atattgatga cttagaatat gaagatgctt tcaaagatga
     3481 agaaaaagtc gttgaaacac caaaagtcgt cactcctgaa attaaagaag aaaagaaaga
     3541 accagttgtg caaaaagtta aaacaacaat ttcgcttgct gacttagaag cagaacttgc
     3601 caaagaaaaa gaagaaattg aacaagcaca acaaaaacgc gaagaacgtc gtagtcgcac
     3661 taaacaagaa gttaagcaac ctgaagttgt tgaaccaaca cttccgactg gaccaaagat
     3721 ggatatttat acggaagaag aacttgcaga actcgacgct gaacttgaag aagtctatga
     3781 agacgatgat ctcgactttg aagatttcga tgaatactac gataactagg agtgtgtaaa
     3841 tgaagaaaat tatcgttcgc cgctgtattg ttacccatga aagtcacgaa aaacgtgatc
     3901 ttattcgtgt tgtaagaacg ccagacggac gtgtggtaat tgataatacg ggaaaagtca
     3961 atggtcgcgg tgcttactta gtgcgcaata aagaagttat tctcttagcg caaaagaaaa
     4021 atgccttaag ccaagcctta aacattgaag ttgaccaagt tatatacgaa aaactccttg
     4081 aggaagttaa tgactaacga acttaataac ttccttcatc tcttaaagcg cagtggccat
     4141 tatcgctttg gcgctgatta cttaagagaa agtaacgctc gtaaaatcgc actagtgcta
     4201 actagtgatg acttaagcga gtacacaaag actaaggtga acaacttcac tttagcgcat
     4261 aacatcgcac aatttgatgt ccctgcgaac atcatgtcta tgctttatcc tggtaaaaca
     4321 attaaagtct tagttgtcac taaccatcaa agcgctcaaa aaatcgcaaa tctaatgaaa
     4381 gaaggtaact gaaatgagta atgaaaaaaa gcgcatcagt aatgttgcac accacagtaa
     4441 aaaacaaagt ggcccttgtg ttgaggggag aaaacttatt tatcaaggcc cgattaaagt
     4501 aagcgattta gcccggcttt taaaacttag tgaggcgcaa attattaaga atttatttat
     4561 gaaagggcaa atggtcacga ttaataatgt gttaagccct gaaacagtgg ccgaactatg
     4621 tttagacttt aactttgatt acgattttaa agaagaaaca gaagatgatt gcgactttgg
     4681 attatcgcat attgttgatg atccaaaaga ccttaaaccg cgtccaccag tagtgacgat
     4741 tatgggtcat gttgatcacg gtaaaacaac actgattgat acaataagga gctcatcaat
     4801 tgtcagtggc gaagcaggta gtattaccca agccatcggt gcttatcaaa aagaatttca
     4861 tggtaaaaag attacattta tcgacactcc tggacacgaa gcctttaccg caatgcgtag
     4921 tcgcgggact aaagtaactg atatcgtcat tttagttgtg gccgcagatg atggggtaat
     4981 gccgcaaact attgaagcaa ttgaccacgc ccgcgctgcc gaagtaccaa ttattgtcgc
     5041 gattaataaa atggataagc caggtgttga tcctgaaaaa gttaaaaatg aacttttagc
     5101 gcaaaacgtg attgctgaag aatacggagg tgacgttatt tttcaaccaa tttctgcgaa
     5161 aaaaggaacg ggtattgatg aactacttga cgcgattctt gtcactgctg aaatgcttga
     5221 attagaggct aatccaaagc gctatgccta cggcacagtt ttagaagcgc gtcttgaacg
     5281 cggtgaagga ccaaaagcta tgcttttaat acaaaacggc accttaacaa atcgtgattt
     5341 tgtcgttgcc ggagcagctt atggtaaagt acggcggatg actaatgagc ataataaggc
     5401 attaaaaagt gctggaccag cgacaccagt cgccattatt ggtcttgacg aagtacctga
     5461 agcgggtgac aatttcatcg cttttgaaac agaaaaagaa gcaaaagaac tcgctactaa
     5521 acgcaaacaa attcgcattg aagagagccg cggtagtagt gaagggatga aacttagtga
     5581 tctccataag caaattgccg aaggtgaaac tgttgtcgtt aatgtgatta ttaaggctga
     5641 taatagcggt agtgcggaag cagtacgcgg gagtcttgaa aagattaatg tcgaaggtgt
     5701 gcgccttaat attattcgtg caagtgctgg ccaaattacc gaaagtgatg tcttacttgc
     5761 aagtgcgagt aatgcagtta ttttcgggtt taatattcgt ccaaataacc aagtgcgtga
     5821 ccttgcagaa cgactaaaaa ttgaaattca tttacatcgc gtaatttatg ctttacttga
     5881 acaaactgaa caagcactaa aaggtttatt aaaacctgaa tttgaagaaa aagttacggg
     5941 ccaagccgaa gtacgagcaa tttggcacgc ttcaaaggtt ggcactattg cgggatgtta
     6001 tacgctgagc ggtgtcattc gccgtaatca aacggttcgg ttgctgcgtg atggcgtcat
     6061 tatttacgaa ggtgaagtag cgacactgaa acgcgtcaaa gatgacatta aagaaacacg
     6121 cgcgggatat gaatgcggga tgactttact taactataac gacattaaag aaggcgatat
     6181 tattgaaggc tatgcgatgg tggaggttaa agatgcctaa aagtcgtaat ttatcacgta
     6241 atgaatcatt aatgctccgt catcttagtg aaattattag ctataaagta ggtgacccaa
     6301 gtttagggct tgctactgtt agtgcggttg aacttacgcg tgactatagt tatgcaaaag
     6361 tatatattag ttttttaggg gatggcgatg ttgaggcgaa gatggaaatc ttaaataacg
     6421 caagcggatt tattcgccat gaactatcgc aaaaaatgaa tattagaaag acaccagaac
     6481 tacgctttat ttatgacgat acttttgaaa gagtagcgaa attagatgaa ttattaaagc
     6541 gtactaaaaa gtaaagttaa ttattaataa gaaaaatagg cctgattata aaggcctatt
     6601 ttatttttaa ttgacaagaa gtgtctaatt ttaaataact ttcttttcaa agtgataatg
     6661 taagataccg gcgtccatat aactttcacc aacgcgttta taccctaagc tttcgtaata
     6721 ttttaataga tgactttgac ccccgatatg gaaagtaagg gggcgatatt taacacttaa
     6781 cttttgctcg acataagtta ttaaagtcat tcctagttta agaccgcgat attcttttaa
     6841 tactgcaata cgacctaagt acgctcttct tgctagtgcg tcgtacttat aacgaattgt
     6901 ggccgcttta ttacctttaa catagagcac taaactagtg taatcttttt catttgtgtc
     6961 atattcgtct tcgactttaa tgttttgctc ttcgacgaat actttataac gaatataaag
     7021 ggcatcatta aagtgtgcag gagtagtaac tttaacaatg acacagtcat catttattag
     7081 cgttgcgtaa tctttgtacc ataaatcaaa ttctttgtcg ttaattaaca tcagttcaaa
     7141 gttagttttc gcttccggat actttttata aatcgcgttt aataaacttt taatgtcatc
     7201 gcgtcttgtt ttattttcat ttggacagtt ataaggaagg agtggtaact ttaaatcttt
     7261 ttgcacacgg attataagtt tttcatcgac aagaataagt ggacggataa aggtgatttc
     7321 cgctctttca agatacatct gtggactaaa agtcgcgaag cgtccgccac caatcatatt
     7381 cataaagagt gtttcaagcg cgtcatcaat atgatgagca aaggcgactt tattagctcc
     7441 taacttatta gcgacttcat taatcgctgc ttttttcatt cgtgaacata ttgaacacgg
     7501 cagttttgat aacccttgtt tttctttctg aacacttaag attttactga cgtcacgcga
     7561 atcattaacg attaagtcat attctctttc cttaaagaaa gcagtataac tactagcgtc
     7621 aaacccgcta aatcctaaat ctaaaatcac agggtaaagc gtaaagtctt tcttactata
     7681 acgcttataa gtccgtagtg cttcaaaaag aacaatggag tctttcccac cactgatgcc
     7741 gatgacaatt ttgtcaccac tttgaattag attaaacttc tcatcagctt tcttaatctt
     7801 tgctagtaat ttgcgaaaat gtttcattgt tttaaaacct gttgctttat attataatat
     7861 aaaagatata tgaataaagg gaaaaacggt attttattag ttaataaaga gcctggttac
     7921 acgagtcgca aagtcggtaa catgctttcg cgtaaactta agataaagaa agtcgggcat
     7981 ttagggacac ttgacccctt tgctagtggc ttactcatct ttgctgttgg tgaaggcacg
     8041 aaagtgttgc cgtatttaga agatgaagtt aaaacttatg ttgcgacatt gtactttggc
     8101 ttattaacaa cgactttaga tccggatggt gaagttatta aaaaagaaaa agttccgccc
     8161 ttttctaaag cggaaattga aaaagttatt aagcaattta ctggtgaagt aacacaaatt
     8221 ccaccgacga caagtgcgat taaaattgat gggaaaaggg cctatgaata tgcgcgtaaa
     8281 aatattgaag tcgacatgcc aagccgtgta gtagaagttt acactataga agtacttgaa
     8341 tataatcatc ctttactagt catagaggca actgttagta aaggcactta tattcgaacg
     8401 cttgctcaag atatagcgct tgccttagga acaaacgcta caacgattaa gctagtgcgc
     8461 acaaggcatg gtgactttag tcttgaaaag gcaaaacttt ttgaagaagt aagtgaagaa
     8521 gatatcattg atccaagtct agggttaaaa aagtttccga tttacaagtt aagtgatgag
     8581 gaagttaaaa gggtgcgaaa cgggatgacg attaaactta atgctagtga agaaattgta
     8641 caaattcata ataatagtgg cgttttgcaa gcattatata agaaaaaagg cgataattat
     8701 atcgcgctac gaggatttaa tttatgattg ctaaacatct tgatttaagt cgtattacat
     8761 ctttagcacc aagcactgca ctagttgttg ggacgtttga cggcgtccat atcggtcata
     8821 agtatttagt ggaagaaggt tttaaagcgg cagataatgt tgcggtttta cttgtataca
     8881 ctaataaaaa acttacaaaa cttcatcaaa aaagtggggt gctaacgagt ttagaagacc
     8941 gtgttgaaat ctttgagaag tttggagtga agactgttta tttattaaat ttaggtgatg
     9001 aattactcaa gcttgaacca tgtgaattta ttaataaagt tttagtacct ttagctccaa
     9061 attctgttat tgttggtact gattttagat ttggtcgggg cgctaaaggc actggcgaag
     9121 atttgcgaga gtttggtaaa aatgtattta cgactagagt tttagcgcca ctttactatg
     9181 aaaatgaaaa agtgagcaca tcactaatta aagagctttt aagtaaaggt aaacctaaga
     9241 tcgcaaagtc attacttggc cgctattatg cgctcactgg gttagtgact aaaggctacg
     9301 gtttaggaaa taaattagga tttccgactg cgaatattgc ccttgaccca agttactttg
     9361 taccagcgca cggtgtatat tttgttcgcg ttaagtttag agctaattat tactttggga
     9421 tggcaagttt agggtaccat ccgacggtta acaaagttaa tgtgccacta cttgaagtta
     9481 atatatttaa ttttaatgaa aatatttatc acgacgccct cactgttgaa tttattgatt
     9541 acttacgtcc agaatttaag tttgaaagtg ttgacacgtt gattaagcaa ttacaaaaag
     9601 atcgtgaaaa ttgtgaattt aagatagaga aaggagaata ttaaatgcaa gtatttaatg
     9661 taaaaaataa tgtttattat attggagtta gtgatagagg acttcgtgtg tttgatatta
     9721 tgatggagac aaaacacggc acaacatata actcttattt aattaaagaa gaaggtggag
     9781 tgacgattat tgacggagtt aaagcaggat ttactgataa gtggttagac aatatacgtt
     9841 cattagtcga tattaaagat attaaatatt tagtcgtgca acatactgaa cctgaccata
     9901 gcggcgctat tcctgccctt ttagcgctta atccaaatat tgaagtttac ggttcattta
     9961 tggcaatggc taatttaaaa gaaattaata atgcaccatt tattagtaat gtaattaaac
    10021 caaaacaaga acttaaactt agtgagcggc aaagtttatt tttctaccct gtgccaaacg
    10081 ttcattggcc ggatactatt attacccatg acccagttaa cgcgattgct tttacttgtg
    10141 actttttagg gcaacatatt gctccacaac aattacttta tagtaaagaa cctaataaaa
    10201 agattattga agaagaaatt aaactatatt acgacgcaat tatggcaccc tttaaaagtg
    10261 acgtgcttaa aagtgtggcg actttaaaaa gtttagatgt aagtatggtg cttaatagtc
    10321 acggttatat tttagacaca aaagaagctg ttgattgggc gattaataac tatattaaat
    10381 ggtcaactgt aaataaaagt gaaaaattaa cggttactgt agtctttgct agtgcttatg
    10441 ggtatacgag gagtatgagt gaagccgtta ttagtgaatt agaaaaagcg agtgccttag
    10501 ttcatgtttt tgaagtacct cgtgatgacc tagcaacaat tgtggcacgc attgaagaaa
    10561 gtcatgcgct cctagttgct tcaccaacct ttgtcgggga cgggattaaa ccagtgtatg
    10621 atgtcttgaa tagtcttaat ccggtgatgt gctttggtaa atattttagc gcttttggga
    10681 gttatggctg gagtggggaa gcggtgcgta attatatgaa ccgcgcaagt gaattacgct
    10741 ttaagaaagt tgatgaaggc tttaaagtgc gcttaaaacc tagtgaaaaa gaattagaag
    10801 atactcgact ctacgctcgt aatttcatca tgaaaataaa agcagagtta aataaataat
    10861 taagcatatt taatttggca atttaccaag taatatgtta atattatgta gcgactttag
    10921 cgctgctatg attcacccct agaattaggg cgatgctaaa gttaagaata aagagaaagg
    10981 ggaataaagt tatggcatta acaaaagaac gcattgccga aatcgttaaa gaattcggtg
    11041 cgaatgaaaa agatacagga tcaactcctg tgcaagtggc gttattaact gaacaaattc
    11101 gcgaattaac tgaacatatg aaagtccaca aacacgacta tcattcacaa cgcggattac
    11161 ttaaattagt tggtcaacgt cgtggtcttc ttgattacta taaccgtgtt aatcgtgaag
    11221 gttacttagc tttaattgca aaattaggct tacgtcgtta aaaaagatta aactgtctat
    11281 gacagttttt tcattattta tgttggagaa aagaggaaaa gatgaaaaaa gtttatgaaa
    11341 tgatgttcca tggccgtaaa attgtcgtgg aaactggcga attcgccaaa caagctgatg
    11401 gcgctgtgtt agtgcgctat ggtgatacgg cagtcttaag tactgctgtt gcgggaaata
    11461 atccgacaaa tttagattac tttccactct cagttgcgta tcaagaaaaa atgtatgcag
    11521 cgggaaaaat tcctggttca tttttacgtc gtgaaggacg tgaaacagaa cacgctactt
    11581 taatcggtcg tttaattgac cgtccgattc gtccgttatt cgcggaagga tttagaaatg
    11641 aagtacaagt tattaatact gtcttaagtg ccgattacga ctgcactcca gaaatgacag
    11701 caatgtttgg tagcagttta gcgcttacga ttagtgatat tccgtttgaa ggaccaattg
    11761 ctggtgtgca agttggtcgc gttgatggta aatttattat taacccaacg accgaagaaa
    11821 acgctaaaag tgacttacaa ctgacagttg ccggtacaaa gcgtgctatt aacatggtcg
    11881 aagctggcgc taatgaagtt agcgaagaag atatgcttga agccttaatg cttgggcacg
    11941 aagcaattaa agaactttgt gcattccaag aagaaattca gcgtgatatc ggtgtcgcta
    12001 aacgcgaatt cccactattt gtacctgatg aaagtttagt cgctgaactt aaagaaaaat
    12061 atacaggtaa actcactgaa gctatcagtg tctttgacaa attagaacgc agtgcagcaa
    12121 ttgaagctgt taaaggcgaa atggttgaag aatatgaagc taaggaatat gaaagtgatg
    12181 acgacaaacg ccgtgttata agtgatgtta aagcgttaca cgaagtaatg gtgagcgatg
    12241 aaattcgccg gcttattact cacgaaaaag ttagacctga tggtcgtaaa gtcgatgaaa
    12301 ttcgtccgct tgatagtcaa gttgacttat taccaagaac gcatggtagt gcacttttta
    12361 cccgcggaca aacacaagtc ttatcagttg tgacgcttgg gccaaagagt gacgaacaaa
    12421 ttattgataa cttaactgat gaagaatcac ggcgctttat gcaccattat aacttcccgc
    12481 cttatagcgt tggcgaaatt aaacgattag gtggtccagc acggcgtgaa attggacatg
    12541 gtgctttagg tgaaagagct ttacttgccg ttattccaag tgacgaagaa ttcccataca
    12601 ccatccgtgt tgtaagtgaa gtacttgaaa gtaatggttc aagttcacaa gctagtattt
    12661 gtgcctcaag tatggcactg atggccgctg gtgtcccaat taaagatatg gttaccggga
    12721 ttgccatggg cttaattaca agtggaccac ttgatggtaa tcatccctat accattctta
    12781 ctgatatcca aggcttagaa gatcacttcg gtgatatgga ctttaaagtt gctggtacta
    12841 gtaaaggaat tactgcatta caaatggata ttaaagtacg cggaatcagc aaagaagtct
    12901 tagccgaaag cttagcgcaa gcgaaaaaag ctcgtgaaga aattcgcgca aatcaattaa
    12961 gtgcgattag tgcaccacgt gaagatgtcg ctgaatttgc accaaagatg ggtaagatga
    13021 caattgatgt tgataaaatc cgtgaagtta tcggtactgg cggtaaagtc attaataaga
    13081 ttatcgaaga taatgatggc gcgaagattg atattgatga cgacggatta gtcgttgtct
    13141 atcacatgaa ccgtgcgacg attaataatg tgcttgccac gattaaagca atcgttaaag
    13201 aagcaaaggc tggggaaatt tacgaaggta aagtaacccg cgttgaacct tatggtgtct
    13261 ttgttaatct ctttggtaat gttgacgcac tagtgcatgt tagtgattta gactgggctt
    13321 atgttgatga tgcatcaaag ttatataaaa ttggtgatac attaaaagtt aaagttactg
    13381 gtgttgatga aaaaggtaaa gttaaagcga gtgtaaaagt ctttaaagct aaaccagaag
    13441 gatacaccga acgtaaaagt ggcggtcgcc gtaataataa atagtaatta ctattgaaaa
    13501 ttctttaagc ctcttaacca tatggtttgg aggtttttta atagatgtaa ataatttaag
    13561 gctttaattc ctaattattt ttataattag tgtatgataa atatatatag aaaagcacgg
    13621 agttgctcta aggtgaattt ttatgtatga cattattatt attggcgctg gggtaatcgg
    13681 cgctttaatt ggacgcgaag caagtaaata tgaacttaat attttaatgc tcgaaaaaga
    13741 taacgatgta ggtaatggca ctagtgctgc taatagcgca attgtgcata gtggttatga
    13801 cccaaagcca gggacattaa aagcaaaatt taatgttgct tcggtgccgc tttacaaaga
    13861 tttatgtagt gacttagatg tggaatttaa gcggattggg agtttagttg tggcacaagg
    13921 tgaagaagaa ttgccccgac ttgatgaatt aaaagcacgc gctgagctta acggcgtgga
    13981 agtcaaaatg ttaaataaag cagaagtaca tgctcttgaa ccaaatttac atgatcatat
    14041 tgtcggcggc ttatttgcac cgacagcagg gatagtaaac ccttttgaac taacggcaca
    14101 cgcgattgaa aatgcaattg aaaacgggat gactttaaag cttaatgaag aggtagtagc
    14161 gataaaaaag cttgaagacg gctttaaagt tacgacgaag aatggtgatt attttagtaa
    14221 agttgtgatt aatgctgcgg gtgtatttgc tgatgatatc attaatatgg taagtaaggc
    14281 tgattttgaa attactccgc gaaagggtag ttattttgtc cttgataaat taacggttcc
    14341 acttgtaaat acagtattat tcccgctgcc taatatacat agtaaaggga ttttagttgt
    14401 cccaacaacc gctggtaata cactgattgg accgacaagt gaacaaatta tgtcaaaagt
    14461 tgatgttggg gcggattatg cttcacttga atttatccgt aaaaacgcta atcggttatt
    14521 aaaagatatt ccgttccata aaacaattcg aactttcagc gggttacggg caacaccatc
    14581 gactggtgac tttattattg aacatgataa atatgttaaa ggtttaatta atgttgctgg
    14641 tattgaaagt cctggtttag caagtgcccc agcgatttcc ctttatgtcg ttaatgaatt
    14701 agtgaaagat ttattaccgc ttaaagtaaa agctaatttt aatccgcgcg ttagaccgct
    14761 gattcgttta caacgcttaa gcactaaaga aattcaagca aaagttaaag aaaataaaga
    14821 ctttgcgaca cttgtctgta attgtgaaaa ggtgacaaaa gccgagatta ttgatgtgct
    14881 gagccgcgct ttaccagtta acagcgttaa agcgatgaaa aagcgtttac gaacaggatt
    14941 tggtttatgt caaggcggat tttgtacggc aaaagttatt gcgattatga gtgaatatta
    15001 cgggattccc gttgatgaaa tagcgtataa cgaaaaagaa agttacgtgg tgaaacatgg
    15061 caaaaaagta tgatctaatt attgttggtg gcggtagtgc tggacttggc gctgctgttg
    15121 cggcttatga cgcaggaatt cataatattt tactaattga aaaagaagca acttttggcg
    15181 gtattttaaa tcaatgtgtg catgatgggt tcggtgtaca tttatttaag aaaacattaa
    15241 caggaccaga atatgcccat tatcttgtcg aaatgttaaa agagcgacct tttgatattt
    15301 tacttagtag taatgtcact gatattagtg acaagcaaga agtgacagta tttgtcgctg
    15361 gtcaaggtgt tatgcttttt aaaacaaaag cgattatttt aacaacaggg tcatacgaac
    15421 gaagtgcggg agcaattaat ttaccaggcg accgcggtgc tggaatcatg aacgctggtc
    15481 aagctcaata cttcctaaat ctcaaaggtt atgaatttgc caaagacgtc ttaattgttg
    15541 gctctggtga tattggctta attatggctc gtcggcttag tttactcggg attaaagtgc
    15601 acgctgttag tgaaattatg ccccattcaa gcggcttaaa acgcaatatt gtccaatgtt
    15661 tagatgactt taatatcccg ctatatttat cacataaagt cactgatgta cgcggtaagc
    15721 cacggataga ggaagttgat atcgctgagc ttgatgataa gtttgaaatc attccaggga
    15781 caactaaaac atttaaagtt gacacactgc ttctagcggt cgggcttatt ccttacgttg
    15841 cgcttagtga taatcttggg attcccacga gtaaatcacg ggggccagta gtaaataaca
    15901 attatcaaac acagttacca tggttttttg tagcgggtaa cgcactccat atccatgact
    15961 tagcggatga tgcttatttt gaaggtgaag aagctggaag agcggctagt ttatttatta
    16021 acggaaagtt aaaagcgccc actaacacgc tacaagtagt gccaggctac ggcatcaatt
    16081 atgttattcc taataatttg gaactcccct taaacactga agaattagtg cttaaattta
    16141 gaccaaaaga aaattatgaa aataaaagga ttgttgtaaa gattgatgat acggtgatat
    16201 ttaagaaatt cttcccgttt ttatttccga gcgaattagt gattttaaaa gtaaaaaaag
    16261 aactattagt cgctggtgaa aaactcatcg tggaggtaag tgatgagtag tgacaaaaaa
    16321 gtaacttgtc ttgtttgtcc gcgcggttgt attatgactg tgagcagcga cttaaaagta
    16381 agcggtaact tttgtgcgcg cggcgtcccc tatgcgctcc aagaagtaaa agaacctaaa
    16441 cgcaatttaa cttatgttgt gcaggttgaa gggcgtgaag cacctttacc cgtccgtact
    16501 gactcgatga ttgcaaagca cttaattccc gatgttgtca agttattacg cactgttacg
    16561 attaagccac cagttgcgtt taacgctatt attattgcca atgtctttga tactggtgtt
    16621 aatatcatta gtagtgccga aattaaataa agtaattact tggtagttta gctaaacttt
    16681 tgttatacta acaaagtaga gatatcacat attattaaca agaggtataa ttatgaacga
    16741 tgaagtaaaa attttcgcat taggggggct agatgaacac ggcaaaaata tgtacgttat
    16801 tgaaattaat aacgatattt ttgtgttaga agctgggtta aagtatccag ataaatcttc
    16861 acctggtatt gattttatta ttccgaatca cgaatactta attacgaata aaagtcggat
    16921 taaaacttat tttattagtc acggccacga tgatcaattt ggtgcattac cgtttatcta
    16981 taaacaagca cccgcaccga tttacacgac aaaagccgcg gcgatgatgc ttgaacgctt
    17041 cactcgtcgt gttggtgtta aagatgttaa gtatgaaata tttacgattg aaccatcaag
    17101 tcagcataat attgctggtc ataagtttac ttttttccaa acaacacact cggcgatgca
    17161 tagctttggg attgcgctag acactagtca aggcacggtt gtgtattctg gtgactttat
    17221 tattgagtat acgaacagtg cacgctataa acttgatctt aatagcattg ccaaaattgc
    17281 cgagaaaaat gtgttatgtt tacttagtga atctagtggt gccgaaaagg cgggatatac
    17341 ttcgcctaca catcgcctag caccaaacat tgtaccaatt tttcgtgaaa caaaggggcg
    17401 tatctttatt tcgctttatg atcaatcaac ttataacatc gaagaattag tgcaagttgt
    17461 gattaacgct aaacggaaaa ttgcttttta cgatccaaat actgaagaat actttagaga
    17521 ttttgcaaaa gctggtttaa gcttaattga tgagcgttat attattagta atgatgactt
    17581 attacgcgct agagaccaag atatagttat tttaatgctt ggcgctggtg aaaaaatcta
    17641 tgaaaagatt attgcccttt ccaataggga aaatcctgat aaaaagattg tacttggcaa
    17701 agatgacacc tttattatgg ctttgcctgc tccagcgaca cttgaagtta ttgccaccga
    17761 agcacttgac tccttataca caactgggtg taacgtcgtc aatattacac gcaaattatt
    17821 tacgaatatg catgcgcaag aagaagactt acggatgtta ctctcacttt taaaaccaaa
    17881 atattatatt cctgttaaag gtttataccg gcaacaaatc gctaatgctc aactcgcgct
    17941 gcatagtggc ttaaagttta gtcatcataa tatattctta ctagataacg gagtcagtgt
    18001 tgaatttaat gatggcgtcg ggcgcgtgaa gatgagtcca cttgacctta ttcctagtgg
    18061 tgatgtgtta gtggacggga caggaatcgg tgatattaat cgcggggtca ttgaagaccg
    18121 ccaacgctta agtgaagacg gaattattat tatggcaatt gccgttaatt atgatgaacg
    18181 cacaattacc gcaggccctg atgtgcagat gcgtggcttt gtgtatgtta aagatagtga
    18241 acaactatta aaacaaattc ggcaaatctt tattaatact gtaagtgaag cactagacgc
    18301 gcgccaattt gatattgatg gcgcggtaaa agaaactgaa tcacgtgttg aaaagtatat
    18361 tttccgtgaa acaagacggc acccgatgat cttaccgctg attcgggtaa ttaacgaata
    18421 acaattaatc acaaaaattt attaattttt gctctctttt tcgagtattc aagtaatatt
    18481 atgtataata ttaagtatga gtgaagcaaa aacaaattta cttatgcaac aacagcgact
    18541 tagccgtggc ttaaaaggca ttattgtcgc gcttttggca cttattggga ttttaaaaat
    18601 ttcgattggt ggcgtcatta atgtcccggt tgcactttta tttggcgact tatttgtcgt
    18661 cttttttgct ttacaagtat tatttgcttt ttatttaatc tttttcccaa aagctaaaag
    18721 taaaaagaga gttaccctag tgtttcttag tatttttggc ttattaatta ttagtagcgc
    18781 cttttacgct ggtaatttac gcaattatat gcgtgacggg acaaccttta aagataattt
    18841 agatgtaatg gtaacgcttg cccgtgatac attttctttt acgccaccgt caaatgctta
    18901 cttttataat tatggcggta ttttaggtga aggcctttta tttgtactta aagatttacc
    18961 agtgtggctc attattatta ttttagtgtt agcaaatatc ggttttgcga caatgttact
    19021 ctggccactg cttgtgaaaa tatatgcagc cattaaagaa gaaattcgtg acttaaagaa
    19081 aagaagacga gtgctccacg aagctggtca attacataaa gaagaagcgc ccacagaaga
    19141 agaccaagtc cccgttacgg aaataatgaa cgataacgaa gtatggggca gtaatttatt
    19201 gcatcgtgtt ggtcctagcg ctagtgaagc tgaagaaaaa ttagggccac cgccagcact
    19261 taatgttgta aaaaatgaaa atagcgggat gcaccgcgcg atgcctagcg aaggtttttc
    19321 cgcttttgat gaacattttg aattaccaac acttgacact aatgaagaag aatttgaagc
    19381 acttgatccg accgttattc gcacgatgga aaaagtcgaa agtgaagaag atttccaaga
    19441 acttgaacgg gctgtcgatg aaaaagggag ttttgaagct gttagtttta ccccgcatca
    19501 tgaagaacct actaatacac ttaacgctga acctaaaaag gacgaaaatg ttttacgcga
    19561 agctctagaa gagcgctatg aacctgaacc gctcccacca cttcctgaac cagtcgtgaa
    19621 gcaagagaat gaacaaaagc cgcgtatttc ttcgaatttc gcaaacttta gattacctaa
    19681 acttgatttg cttgatgacg ctattgataa tggtgaagaa gcacaaaatc gcgaatttgc
    19741 tgcggaatat gaagctaaga ttaatgcgtt ctttacggac tttaatgttg atgctcgggt
    19801 gtttagctgg acgattggct caacatttac ccgctttgaa attcagcttg gtccgacaga
    19861 aagtgttaaa gcaattaatc cgcttttaaa tgatatttca atacgactaa atggtgcttc
    19921 tgtgcgtttt gaagaagttt taccaggtaa agcaacaagt ggcttagagg tagcgaacaa
    19981 atttagaacg aatgttaatt ttaaagattg tatgcataaa attgtcgcga ataagcgtaa
    20041 gaaatattta attccgcttg gtaaagatgt cgctggtaat gttattttag cgccgtttac
    20101 cgatctcccg caccttcttg ttgccggggc aacaggcagc ggtaaaagtg tgtttattca
    20161 tactgtatta acttccctta ttatgatgca ttcaccactt gaattacgat taatattaat
    20221 tgacccaaaa cgcgttgaac tttctgctta taacaatatc ccgcatttaa tcacaccgat
    20281 tattaaagag ccaagtgaag caaaagtagc gcttgaccgg ctcattgatg agatggaaaa
    20341 tcgctataaa ctttttgaaa aaacgggggt aggtaaactt agcgattata acgaaattat
    20401 cgaagagatg ggtggcgaaa aattaccggt tatcgtcgcg atagtcgatg aatttgccga
    20461 tctttttgaa agtgaaaaag aaatatcaaa cctcgtatta cggcttgtgc aaaaaagccg
    20521 cgcggctgga attcacttat taattgcaac acaaagacca agtgtccaag ttattactgg
    20581 taatattaag gctaatattc ctagccgtgt tgcctttatg acggcaagcg tgactgatag
    20641 tatgacgatt cttggttgcg gtggagcgga aaacttactt ggctacgggg atatgttagt
    20701 tgatagtatt acgactaatt atcgcggact attacgtgta caagcgcctt atgtacaggt
    20761 gcgagaagta ctccgcgtca ctaattattt acgcaataac tatatgaccg atttttatcc
    20821 ggaattttta gatttaaaag aaaaagttaa tgtctatggt ggtggcggtc ccgttattag
    20881 tgatgacctc tatcaagcag tgcttgactt tactttaacg caagatacga tttctatcag
    20941 cagattacaa caaaacttta atttaggatg gccgcgtgcc cgtcaacttt atgatcaatt
    21001 aatgcaaaat ggcattattg aaccccctga tgaagctaat agcgctaaag gggcggttgt
    21061 tattgccaat aagagaggta attaattatg tcgattggga ttgatattgt ttatattccg
    21121 cgctttgcgg gtaaagaaaa acttgcgaca cgcatcttat cgaaaaatga attggaaata
    21181 tatcatatgc gaccaaataa agaagaattt cttgcagggc gttttgccgc taaagaggcg
    21241 tttttaaaag caatgaaaga agggataggc cgcgttgctt ttcataaaat tgatgttgtg
    21301 tataaaaaag acggacgccc tgaaattcat catgacggta atgtttatgc tgtgtcaatt
    21361 agtcacgacg gtgattatgc ggtcgcagta gtacagatta aggagtagta tttatggcat
    21421 ttggacaatt tagcgcacat ttaattgcgc cacgggtaat ccgcgtggtg ctctatagtg
    21481 ataaatatca atatgaacaa acacgtgtga caatttatga tgataacgat aaattagtgc
    21541 cgtttagttc gaagagtaaa gtcgtacaat ataaagttac tgcttttgaa ttaacactag
    21601 aacaaccttt tgtgttagga caaaattata aagtttattt acaaaatttt gggcaagtgc
    21661 cgcttaatgt aagtgaggca acaacttttc ctaattttga cactgattat tattacactg
    21721 gcccgcttgg tgcggagtac actaaagaac aaacaacatt tagactctgg gcaccgctag
    21781 cgagtcgcgt gattttaaaa tatgaagtac caggtgaaaa acatttctta tatcgcaaac
    21841 ttaaacgcgg tgaacgcgga gtgtatgaat acacgcttga aggtgatcat gaaggaattc
    21901 gttatactta cttagtgcta aataacggtt tagaagagga agtaactgac ccttacgccc
    21961 ttagcagtgg accaaatggt gcttttagtt atgtcattaa tcagaataaa tatgatgata
    22021 tgaatatgca tgacgataaa ttaccgccat ttactaaacc taccgaagca ataatttacg
    22081 aaacaagtgt gcgcgatcaa acaattgatg aacgcaccga tattgagtat ccggcgacat
    22141 tttacgggat gattgaagaa ggacgcaagg taaaagatac ttaccctgct ggatttgatt
    22201 acttaaaaga tttaggattt acacacttac aattaatgcc tgtttatgac tttaaaaccg
    22261 ttgatgaaac gcgtaaatgg caaagctata actggggcta tgacccgcag caatattttg
    22321 tgttagaagg aagttatgcg cgtgatccgc ttcatcccta taaccgtatt agtgatttta
    22381 aactaatggt cagtcgttat cacgaagtgg gaatccgtat tattatggac gttgttttta
    22441 atcatgttta tgaccatgaa acgagtgttt ttgaaaagat tgtaccaaat tactacttcc
    22501 gtaaaacaag tgaaggtgtg attagtaatg gcagtggttg cggtaatgat ttagcaacgg
    22561 aacgcccgat ggtacggcgc cttgtcgttg atgcctgtcg ctattacgcg gaaaaattag
    22621 gggttgacgg gttccgtttt gacttaatgg gtttaattga tattaaaaca atggaagaag
    22681 tcacaaaagc cgtgcgccaa gtaaaacctg atttcttaat tttaggtgaa ggctgggaga
    22741 tgatgacccc gcatcctaaa gaaaaattag cgcatcacgg taacgctagt aagacaccag
    22801 agattggctt ctttaatgat gcgttccgcg aaattgttaa aggcggtagt tttgccgata
    22861 accttcatga acgcggttat atgttaggcg ccacaaatta tcgcttaggc ttttattatg
    22921 cttacgctgg tagtgtgctt aatacaacat ttacgccccg ctatcttaac gctggacaaa
    22981 gtatcaacta tgttgaatgt catgataacg gtgttttagt tgataaaatg acggttagta
    23041 acgctagtga atgtaaggaa actcatttag cgagactcct tagtatgaat acgattgtca
    23101 tgctctcttt tggtgtccct ttcttccatc gcggacaaga aattggtgtg tcaaaatatg
    23161 gtgacttaaa ttcatataag agcagcgata aagttaacca attcccgtat actttagcct
    23221 tgaaacgtaa agaaatggtt gaatatttta aagctttaac agcgctacgc aaagattgta
    23281 aatttttaca tgaaactgat cctaaagtca ttaagaatat aattgaattt aaagatttac
    23341 caggtggcgc aagtcaaatc gtgtacaatc ccgatatgga tttaagcccg tatagtcact
    23401 ttaatgtgta tgttaatccc actagtgaac caatctttat tagtttagaa aaaccactta
    23461 aaacaatttt aagtagtggc ggatatatcg ccgctcgctc gaaagaatat ttcgaaagaa
    23521 cgatggttgc cccctatagt gtaattgtgc taggactacg gaaaggagat tctggcccaa
    23581 aataaactaa taggaggaag gattatgtta acatccgtat ttatcgttgg taccctaggt
    23641 aaaaacgaaa atgattatcg ctatttactt gtcgagaaag tgccagggtt agattatgag
    23701 gatgatgaag aaagagcaaa gtatgactac tttaaagtca aacattggtc gaatacacct
    23761 tcagccttta ataggcttgc cgaaggacgt aaagttgcac taaaaggtcg actcgaagaa
    23821 attgaaggag aaacgtatat tattgccgaa ttatatcgtg agttttaaaa agagattatg
    23881 attaagtata ttatttggat agtgaaggta gcaatcccct taatgtgggg ttattttaca
    23941 tggatgcgcc cctacgcacg gcgtcctgat aagtaccctc ccgaattgcg gatggcaaaa
    24001 ggacaaaaac ttgcacggcg tgtgctacgc attttaaaag ttgatgtgga gattcgtaat
    24061 atgccagact ttaagcgtga cgaaatttat tacatcgtta gcaatcacac gagtgtgtta
    24121 gatcttgtcg tcttacttgc gtatcttcca atccccgtgt cctttttatc aaaagtcgaa
    24181 aataagaaaa ttccgtttgt gcggacactg atgcgggtgt acaaaggttt atatcttgaa
    24241 cgcaataact tacgccaaga aattaaagta atgcaaatga ctcgtgactc tcttgctaat
    24301 aaagaagtaa gttggcttat tttccccgaa ggaacgcgca gtaaagatta tcacggtaat
    24361 ctttcagatt ataaagccgg tgcttttaaa gctcccttag cgacaaatac aacgattgtc
    24421 cccgttgtga tgtggggtaa ccaaatggtc ttaccactaa gaacaagagc gcggcgttat
    24481 aaagtaattg tcgaattttt accactttta aaacctgaag gcacaacgct tgaactcgct
    24541 aataagatat atgaagcaag taatgcgacc gctagtaaac ttcgtgaaga atacggaagt
    24601 gtccaaaaaa ttaataaata cgggcgtaaa attatgacgc tgcaacttag tgaaaatatt
    24661 aaaacatcta aataatttgc ttaatttaat gaaaaaaatg acgataaaaa gtatagttta
    24721 ctctatctga gtagagtaag cgctttttag tatggtaaaa tagttttaat aaaggagata
    24781 ttaactatgc ggatgtatga cattattttg aaaaaacgcg atggtttcgc acttactgaa
    24841 gaagaaatta aattctttat taaaggagta actgacggga ctattcctga ttatcaaacg
    24901 agtagtttac taatggcgat tgtgtggcgc ggaatgaata aagaagaaac cgccgcctta
    24961 acactagcga tgatgcatag tggtgatgtt attgatttaa gtgccattaa aggtgttaaa
    25021 gttgataaac atagcacggg tggtgttggt gataaaacaa gcttagtgct tggtccgctt
    25081 gtcgctagtt ttggggttaa agtcgccaaa atgagtgggc gcggcttagg gcataccggc
    25141 gggacacttg ataaacttga aagtattcca ggtacgcgta ttgatattcc aaatgatgac
    25201 tttattcggc aagttaatga agtaggttgt gcgattattg ggcaaacggg gcacttagta
    25261 cccgctgata agaagttata tgcgcttcgt gatgtgacag caactgttga agctattccg
    25321 cttattgcta gttccattat gtcaaagaaa cttgcttccg gcagtgacac gattttactt
    25381 gatgttaaat ttggtagcgg tgcgtttatg aagacactaa aagacgccca agaattagcg
    25441 cgagcgatgg ttgatattgg taagagttta aaccgtgata cacgggctat tttaacggat
    25501 atggatcaac cactaggcct tgcagtcggt aatgcacttg aagtcaaaga agcaatcgcc
    25561 acgcttaaag gcgaaggacc gcgtgactta agtgaattat gtatcgaagc agcgtgtatt
    25621 atgcttgaac aagcaggtgt cgtaaaaaaa gcacaagacg ctcgcaaaga agttgaaaat
    25681 aaaattaagt caggtgctgc gctccaaaaa cttgtcgata tggtgaaagc acaaggtggt
    25741 gacgagagtt atattactaa cccggaaaag tttgcgatta gtcaaaatgt gtttgaagta
    25801 ttagcgacta gtgaaggtta tgttgaacat attgatagta tggcaattgg tatttctagt
    25861 atgaaattag gtggtggccg cgaaacttat gaagatgaga ttgatatgtc cgccggaatt
    25921 gttttaaata aaaaagtagg agataaagtg aaagttggtg aacgcctagc gctcgtatat
    25981 acgaataaag ctgatgaagt ttctactcca atcttagttg atattcatca tgcctttaaa
    26041 cttagcgata agcatattga tgtcccacca attattcatg attatattaa gtaatgcgtg
    26101 ttgttattca aattgttaaa gatgctaaat tagaaataga taataaagtg tattcaagta
    26161 ttgattacgg aatgctttta cttgtatctt ttacgcttag tgacactaaa gaagtagcga
    26221 aaaaaatggc ggaaaagata gcacttatgc gtatatttcc cgatgaaaac ggaaaaacta
    26281 atttaaatat taatcaagtt aatggcgaag ttttaagtgt tagtcagttt actctttatg
    26341 gtgaatttaa tagcagaaga ccttctttta ccaaagtctt aagcggggaa gaaagtagtc
    26401 agctctacga ctattttaat agcgaactta gaaaaatgtt aaaagttaaa actggggtat
    26461 ttggcagtga tatgaatatc acattcacta atgttggccc agtgacttat gtggtggaaa
    26521 gcgatgaaaa aagataaagc taatattaag gtaatggttg gccttagtgg cggcgttgat
    26581 agtgccgttg ctttagcgct cttaaaaaaa gaaggctacg atgttgaagc ggcttttatg
    26641 cggaactggg actcactttt aaataatgat gttagcggca accccacgct ttacgaccca
    26701 acttgtccgc aagaagttga ttataatgac gcaaaagcag tagcagaaac gcttggcgtt
    26761 aaacttcatc gtgtcgactt tgttaaagaa tattgggaca atgtttttag ttatttttta
    26821 agtgaatatg aagttggtcg caccccaaat ccagacatct tttgcaataa atacatcaaa
    26881 tttgacgcgt ttttagcgtg ggcattagag atgggcgcag attatattgc aaccgggcac
    26941 tacgctaagc tagaagaagt aaacggcgaa cttgttatga aaaaaagcgc agataaaaat
    27001 aaagatcaaa cttatttctt aagtcagctc tcacaagcgc aacttaagcg tactttattc
    27061 ccacttgggg atatccctaa aagtaaagta cgggaaattg ctgaaaagct tgatttaagt
    27121 cctgctaaaa agaaagatag taccgggatc tgttttatcg gtgaaagaca tttccgtgat
    27181 tttttaaaaa attacatacc agcgcaagat ggtgtgattg tcgatattaa taccggtaaa
    27241 gagataggga cgcacacagg agtatattat tacaccttag gtcaaagacg tggtttaggg
    27301 attggtggtc ttaaagatta cccagaaaat aaacgctggt atgtatgtaa aaaagatgtg
    27361 gtaaataata ttttatatgt cgctaatgat gatgaagatg aacatttaaa aagtgataag
    27421 attactttga ctgatgttaa ttttattaat gaagaattaa agagtggaac tcccttaaat
    27481 gttaagctcc gttatcggca aaaagacttg cctgccactt attataaaaa taaagacggt
    27541 gcctacttat tattccccaa accttatcta gccgtgaccc caggtcaagc agcagttttc
    27601 tatattgacg aagtgtgtct tggcggtgct attattggtg acttatttta taaaggcaaa
    27661 aagattaact aatcgttaac tatgctataa tattatcgaa atcaataagg aaactaagtt
    27721 ttacaaactt aaaagcgaga gttagttggt gaaaggactc aaaagtaaaa cgtgccactt
    27781 ccttcgagcg actaatcatc gccggttaat taccgttaca aaattaaaat aaagcgcatc
    27841 acaacgatga attaggatgg taccgcggcg agagtcgttc ctatgttagt tgaggttttt
    27901 tatgaaaaaa ttaacaagta atgacattcg caaaatgtgg ctgtcctttt ttaaaagcaa
    27961 aaatcatgcc gtagtggaag gcgctagttt gattccggtt aatgataaga cactactttg
    28021 gattaattcg ggcgtcgcag cgcttaaaca atattttgat ggtagtaagg taagtgcgga
    28081 taaacgttta acaaatgtcc aaaaaagtat tcgcacgaat gatattgaaa atgtcggtaa
    28141 aacaaatcgt caccacacct tttttgagat gttaggtaat tttagtattg gtgactattt
    28201 ccgtacggaa gcaattacct ggggctttga atttttaacg agtaaagatt acttagcttt
    28261 accgcttgag aaactttatt ttacttatca cccaagtgac acagaaacgc gtgacttatg
    28321 gattagttta ggtgtgccga agagtcatgt tatcgctaat gaagataatt attgggaaat
    28381 tggggaagga ccgtgtggtc ccaataccga aattttcttt gatttagggg aaaaaattga
    28441 tgcgcgtggt ccagaattat tagcaagtga tattgataat gacaggttcc ttgaaatttg
    28501 gaatattgtg tttagtcaat ataacgctaa agcgggagta aagcgcgaga attacgaaga
    28561 attaccgagt aaaaacatcg atactggcgc tggtttagaa cggattgcta gtgtattaca
    28621 agaagtgcca agtaactttg agactgattt atttatgcca attattaaag aattagaaaa
    28681 gttatcgggc gttaaatata gtgatgatat cgttagtttc cgtgttattg ctgatcatat
    28741 tcgtacatca acattcgcaa tagcagatgg cgctagtttt agtaatgaag ggcggggtta
    28801 tgtcttacgc cgtgtattac gacgcgcggt gcgttatggc caaaagctcg ctatgaaaga
    28861 acccttcctt tacaaattag tgcaaagtgt agttgatgtt atgaaagact tctatccata
    28921 tttagaaagt aaaatagacc atgtcgaaaa acttattaaa gccgaagaag tacgcttttt
    28981 aaatacactt aatcacggtg aagaattatt acgcggtgcc ctatcaaata gcaagaacat
    29041 cagcggggaa gttgcattta aactctatga tacttttggg ttccccattg atttaacact
    29101 agaaatcgct agtgaaaatg gcgcgagtgt cgatgtgaaa ggctttgaag ttttgcttga
    29161 agaacaacgc gaaagagcac gtgctgcgcg taaaaattta aatagcatgg ctagtcaaag
    29221 tgaagactta ttaaagtttg atcagaagtc aaaatttgaa gaacaaaaat ttagtgttac
    29281 cgcgactgtg attggcttat ttaaagatgg tgttaaagtt aatgaaagtg cagaagatgt
    29341 gagtgccatc tttgatgtta ccccctttta cagtgaaagc ggcggacaag ttagtgatag
    29401 cggttttgta agcaacaaag aacttcgcgc tgtcgtcagt gcgatgagta aagcaccaca
    29461 cggtcaacat ttacatcatc taacgcttga ttacggcgta attaaggttg gtgataaagt
    29521 acaattagaa atcgacgggg tagcgcgtcg ctccattatg cttaaccaca gtgccacaca
    29581 cttattacac cgtgcactta acgatgtatt aggtagtgaa gtaagtcaag ctgggagtta
    29641 tgttggtcct gattatttac gctttgactt taattataat acccgtgtaa ctgacgaaga
    29701 attagaaaaa attgaattaa ttgttaataa atatattagt gactcagtcc cattaactgt
    29761 taaatatatg actttagcgg aagcacaaaa gcttggtgca aaagctttat ttatggaaaa
    29821 gtatggtgac ttagttcgtg ttgttacttt tgccggaata tctgttgaac tttgtggtgg
    29881 gacacatatt gataatacaa gtaagattgg tacttttgta attgtaagtg aaagtagtat
    29941 cgctagtggg gtgcgccgga ttgttgcttt aaccggatta gcggcctacg aacacctaaa
    30001 aacatattct gacactgtga aaagaataag taaagagctt gggcttaatg ataaaaaaga
    30061 tgtcgtgcca catgtgcgca ctctagtaga aaaaaatgcc cttttaaata gtgaagtcac
    30121 aatgcttaaa acagctgaac tccatcgtac tgcttccgct ctaacaccga aaaagcacgg
    30181 tgatacgaat atatatttag aatatttcac tacttcaaat cgtgaagact taggagttat
    30241 tgccgataaa gtgagaagca ctgatgagtg cgctttactt gtgttagttg gtaataatac
    30301 aaatgacttc ccactgctta ttagtgtgag taaagagtta tcaaagtcgc taaacgctaa
    30361 taaagtaatg cgcgcattaa caagtgcttt tggtggacgc ggtggcggcc gacctgactt
    30421 tgctaacggt gtgctcttaa acttaccaga aaaaacaaaa gcattagcga caatattaaa
    30481 ggtgcttgat gagtaaaata ttaggcttag acttaggaac taaaacgctt gggatagcga
    30541 taagtaatgt tgacgcgaca tttgcagttg cttatgaaaa ttatgtgttt cccccgcatt
    30601 actataaaaa ggcgcgtgcc catgtcttag caatatgcgc taaagaaaac attagtgaaa
    30661 ttgcgcttgg ctacccgctt aacactgatg gcactgttgg ggaaagagca aagtctgcgg
    30721 aaagattccg tgatgatctc ttgcgtgaag gttcgccgcc tattgtatta gttaatgaaa
    30781 cttacacgac gcttgcggct accgaagaac ttgaacgact cggttataaa aaggagcaaa
    30841 ttaaagcaca gattgacgct gtcgcggcaa aatacatatt agaaacttat ttagaaagga
    30901 aaagaagcaa tgaaagaaaa tgatgtgatt gttattacaa ccgaagaagg cggagaagaa
    30961 gagtttatta ttctttttac gtatgaaaat gaagaacgag gcacaaaata tgtctttttc
    31021 tatgatgaaa ataatgatga agaagtgatg tgcgcgaagt attttgatga cgggacatta
    31081 gaatatgttg aagacgaaga agaacttgcg gaaatggaag aagtattccg cgcctacgaa
    31141 agcgaattag aagagtaacc gcttcccttt tgtttttggt tgagaattat aagcattatg
    31201 ttataatgca ttagcaattt atataggaga aatgactatg actaataaat atactttaac
    31261 ccaagaaggg gtcgacgcct tagaagaaga attaagtgtt ctcgtaaatg tagaacggcc
    31321 tctagtgctc aaagaaatcg aagaagcccg tagttttggt gacttaagtg aaaacgcaga
    31381 ttacgacgct gcccgtaata aacaagccaa tatcgaaagc cggattatgg aaattgaaca
    31441 cattttagcg cataaagaaa tcgttgaata ttccaaaacg agtaaaacag ttgccgttgg
    31501 gaactatgtt gaagttcatg accgcacaaa tgatgatact ttctcgctcc gtattttagg
    31561 acaatttgaa ggtaaaccac ttaatggtac agtttctgtt gatgccccga ttgcccaagc
    31621 cattttagga aaaaaagttg gtgaccgcgt aaacgtaaaa gcccaacctc cctatgaagt
    31681 ggaaatttta aatatttcga ctgaaccaat cgaataatta atatttttgt agtaaaaaga
    31741 taaataactt cctacttatt agtaggaggt tttttattta tgttaaagct aatgattggt
    31801 gtagtgatcg ctacagttat tgtcatcttt gcctttagtg tgattaatag caatatgagc
    31861 gctaatccgc aaggacaaag ttcaatgcat gttattgatg ataacttttt atcaataaca
    31921 gtgacgggac aagtcgtaaa accaggaaca tatgtgatga aaaaagacga aacaattggt
    31981 gacttaatta tggcagcggg cggaccaaca agtaacgctg atgcgcgctc ttatattgaa
    32041 gacacagtgc ttattgctgg tgttagttat tacatcgctc cgattaatga cttagatgat
    32101 tactgtgggg agatgccgct ccataaagtt aatattaacg ccgaccccaa agataaacta
    32161 atggaagtta acggcattgg tgagacgcta gctggtgcga ttattcagta tcgcggggag
    32221 cacggcgaat ttacttattt agaagaacta atgaaggtaa aaggcattgc caaagcaaca
    32281 tttgaaaaaa ttaaaaatca tgtaacttta cgatgaattt attattcatc tttctatcat
    32341 tttgctgcgg acttttagca tttagacttc cactttttat ccttcctttc ttgttgcttc
    32401 tagtccgcac ttttaaaaat aaattgactt ttacttttgt tattgtcttt tttgctttag
    32461 gtctaattat aatgattata agtaacttta ttagtaagtt accttttgaa agtagttttg
    32521 ggctcataac taaagcgggc actaatcata ttatagtttt aacagtgcgt ggaaaatttt
    32581 atgtgcggtt agaaaataat aaacttgtag aaggggacat aattttactt aacggttata
    32641 aagtagctta cttttttaag cggcttgaag gagaatttaa ttacgcgaaa tatttagaaa
    32701 ataagggaat taaaagtgcg cttaatcttt cgttttatga actaaaagta ccgtctttat
    32761 tacgactatt taataagttt acagttaaaa aaaatactta tcctccgcat cttgaacaca
    32821 ttaaaaattt attattaatt aaaacaataa attacgatga tccctttatt catgaattaa
    32881 tacggttaga cttattattt ctaattagtc taacaggagc gcatctctcg tttttacgcc
    32941 gtgttgtaaa gaaaataagc agtgtgttcg ttaacgagtt tcttcaagaa gtcatcagtt
    33001 acttatgtct tattccgtta tttttactta atattactaa gtttgctttt tatcgtatct
    33061 ttttaacggg aattttgcgt cttattaata agaagcacct taatgcttac tttaatagtg
    33121 tagaattaac gtgtattgct gcttttttct ttttagttat taatccttat tttgtttttg
    33181 atcccgcatt ctacttagcc tacttattaa catttctttt tcaaatggtg cgtccgtctc
    33241 gttcagtaaa aggaaggtta caaagctctc ttatcgtgat gtgtgttatt gtgccttata
    33301 acatatttac aagcggaaca tttaatttag tgtatatctt tattagtctt ttattagtgc
    33361 cgcttattgc tgtgtggaca ctagtttact ttgtaacact ttttattgct cctttgcaaa
    33421 atgttggcat aaatacactt aatcttattt atcacctagt tgttttgatg aaaaaaatta
    33481 atatcacact atattttggt gatttaaatg tgctttctaa actcttatta gttactttta
    33541 tcagcgtgtt gctttatgct tttaacgcta agattaaacc attacaaaaa gatattacgc
    33601 tattaataac gacattgctt ttattaaact tattaccact taatcattat tttgaatata
    33661 gtattagttt tataaatgtc ggtcaaggtg atagtacttt actacggata aaaaataaat
    33721 atgtattaat cgatacgggg ggattaactt ataaagatgt tggcaatgat attttaatac
    33781 cctacttaaa acgcaataaa atttatcgtt tagattactt aattattaca catgatgact
    33841 ttgatcactg tggtgctaaa gaagcgttaa tgcataattt tacggtgaaa aatgttatta
    33901 ccgaaagaca atatttcccg cttaatgtta acggaataac attagagaac ttaaataata
    33961 agtattatga tgatgtaaat cttgattcat taatcatcta tgtgcctttt ccaaagttaa
    34021 aaatcctttt aatggcagac gccgggatac caaatgaagt agatttaata aagacttatc
    34081 ctactttaga ggttgacgtt ttaaaggttg gtcatcacgg ttcaaatacg agtacaagcg
    34141 acgactttat tcgtcattac aagccacaag tagcaattat tagctgcgga tatcaaaata
    34201 aatacggtca cccccatcac accgtacttt cgactttaca aaaatatgat gtgaaaataa
    34261 aaagaacaga cactcttggt acaattacct taaaaacttg tataatataa ctatgttata
    34321 tctctttgcg gctaatgaat atgcactagt ggaaaaggta attcggaaaa cggtagatgc
    34381 tttattaccc gaacgaaatg cttttaacta tgtgcgttac gacatgcgag aaacgccttt
    34441 tagtgaaatc attgaagatg cactaagtta tgcttttgat agcagtgtac gtgttattgt
    34501 tatcgaccac gccagtgtgt taacgacaag taatgaaaaa agtattaata aagatattcc
    34561 tgacgaacaa attgatttgc tttcgcgtct aagtgaagat acgcatctta ttttcatcgc
    34621 tcgttcttca caaattaacc cgaaacataa aattattaag tttattgaaa ataacgggaa
    34681 aataacggtt gaaaaagata ttgataaaaa tgattggcaa cgctttgtgc gtgcttactt
    34741 tgctaaagaa aatttaccga ttgatgatga ggctgcaagt gaattactta gccgcgtcac
    34801 taatctaggt tcttttttac aggaagcaga aaaattagcg atttatggta aaagagttac
    34861 tttgccactg gttgaagaac ttgtaacacc gcttttagaa gaaaatagtt ttgcgctagt
    34921 taatgccttt atcagcggtg acaaagcgaa gactattaaa atatatcaag attttaaagt
    34981 gcaaaaccaa gaaccagtcg cctttattgc tcaagtcggt aatcagttta gaacatatgc
    35041 acaaatttat atatgtcatg aagaagggat gacaaacgaa gaaattgcta gttttttgaa
    35101 aattcatcct taccacgtta aactagcgat ggatcaaaga cgacgcacta gtttagaaga
    35161 agtatttaat attttactcg cgcttgcgga tttagattat aaaataaaaa gcggccaaat
    35221 tgaccgtttt tatggatttg aaatgtttct tcttaattac taagcagaaa gttcattaat
    35281 ttgacgcgct aattcgctct tatgacgagc aactttattg cggtggaaca caccacgacc
    35341 tgctgctttg tctaataaag agacggcttt tttatattca gcttcggcta aggcgacatc
    35401 ttttgcatca atcgctacgc gaactttttt aagttgagtt cgcaccattg atttaaaatg
    35461 ggcgttgcgt tggcgcgctt tttcattggt cttaacacgt ttaatttgtt gcttaatatt
    35521 tggcatacta ttacctcttt caataataat cttaaatatt atataaaatt ataataattt
    35581 tagcaaataa agagtgcaat aatttttcag taattgcgcg tttctattca ttatattcat
    35641 taaatttctt c
//
LOCUS       DAOSTA010000007        34772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_1370_length_34772_cov_8.716191, whole genome shotgun sequence.
ACCESSION   DAOSTA010000007 DAOSTA010000000
VERSION     DAOSTA010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 34772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34772
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1370_length_34772_cov_8.716191"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..374
                     /locus_tag="PLU08_01385"
     CDS             141..374
                     /locus_tag="PLU08_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70395.1"
                     /translation="MSLKKEPLDTYLYREFSIAETTHKSGERKWGEGMSKNSKKGREN
                     PICIKNNYEFKYEEFKEYKGKNGRFELLVVEIQ"
     gene            805..2127
                     /locus_tag="PLU08_01390"
     CDS             805..2127
                     /locus_tag="PLU08_01390"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ70396.1"
                     /translation="MKREIMQDLISWKNKRNRKPLLLTGVRQCGKTYILRKFGEEHFD
                     NYVYLNLERDPQIAAVFNYDYDTKRILQDISNLSLLPEIIPNKTLLIIDEIQFNPKAI
                     TALKYFAEDMPELCIVGAGSLLGVSLREEGASFPVGKVDRLEMYPMSFKEFLLESKSR
                     RIVNSIENFDITKLLPDYILDNLNREYTNYLLVGGMPEAVKVWFENHNLAEVNEIQDN
                     IIAGYENDFSKHSPKNELTNIELIWRSIPEQLAEDNNKFVFSRVKKSARAKDLEKSIG
                     WLIDAGLIRVVTKVDNPQVPLSFYSNSSFYKIYLCDVGLLSRMAGFTLKSLLEKDEST
                     GGFRGSLSENFINNELVSLGYVTYFWRSKNSAEVDFLIEDDGRVIPIEVKANVNTKAK
                     SYNNFIKKYATKIGFRFSLKNSGENKVGVTKTYSLPLSLVFRLKDYLN"
     gene            2291..2791
                     /locus_tag="PLU08_01395"
     CDS             2291..2791
                     /locus_tag="PLU08_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOQ70397.1"
                     /translation="MSKITHVKNFVGIYSIVFAVLGFVALIIDKTHEPIIHILLTLFF
                     ILGVIVHVPFLLKIHLVSMIVMGFLHGSLLIMSIIVMFHDCLTGLFGFGLFILPLVVS
                     IVYFVMYSNDKKKQKNLLVAALDSNNTKESNTLNDELNALLASKNRGEITEEEYLAKK
                     KDLIGY"
     gene            complement(2788..3510)
                     /locus_tag="PLU08_01400"
     CDS             complement(2788..3510)
                     /locus_tag="PLU08_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70398.1"
                     /translation="MKKAKPIKFKQKRKRWYRALKRIIKVRYKEPKFIFLGEKPTTNA
                     IILSNHVGTDAPMSLEIYAPFHLRMWGTHEMNSGLRKMYRYQSLVYYHEKKGWNLHLA
                     RLFCLLASPLTNLFYKGLRLISTYQDLRLKKTVKESLEAFKDGDNIVIFPEVSDEGYL
                     DELKGFHGGFCILAEIAYKHGIDTYLYASYFKIKEKVYLFDKPILYSELKKIAPTRLE
                     MARYLVDKCNALGKLDLNKIKS"
     gene            3780..4370
                     /locus_tag="PLU08_01405"
     CDS             3780..4370
                     /locus_tag="PLU08_01405"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOQ70399.1"
                     /translation="MFQNFDGGYIEVVCGPMFAGKTEELIRRIKRLEYAKQSYIIFKP
                     KIDGRYGDNVIQSHNNNRREAVNIEKAREIYTHLKDKTVEVIIIDEAQFFDEELPDVV
                     DDLADQGYRVIVGGLDRNFRGEPFGTMPEILALAEVVTKLTAICPVSGKPATRTQRII
                     DGKPAHYNDPLVLIDAEESYEPRSRAQHIVPGKKKK"
     gene            complement(4405..4761)
                     /locus_tag="PLU08_01410"
     CDS             complement(4405..4761)
                     /locus_tag="PLU08_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70400.1"
                     /translation="MKHITIVYDEKLRSFGIKVIKVRPGAFKTDMQINVTHKFEKVFA
                     YTKDFKSPLTKMQKMMLTELERAKDPRKITKTFLKAIYKKRPKTVYKVNNSFKMKLLN
                     ALSVKVQDFILGLFFK"
     gene            complement(4828..5211)
                     /locus_tag="PLU08_01415"
     CDS             complement(4828..5211)
                     /locus_tag="PLU08_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ70401.1"
                     /translation="MKYALITGASGGLAHNIITAIKDDFFLILTDINPAISDKYNDLP
                     NKLIVVSDLTDKDSINNLFDKILKITDKLDLVIHFAGILEIGPIMEVKVEALETLMQV
                     NLFAVYFINQKAFPLLKKLKVALFI"
     gene            5331..6008
                     /locus_tag="PLU08_01420"
     CDS             5331..6008
                     /locus_tag="PLU08_01420"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOQ70402.1"
                     /translation="MNVFVYIFFGLFIAVTIAELIMAFNEIEKARMILKPFSMFFLAI
                     AALIAVPNHPLIYLGAFLGMVGDIFLLWPKNKKMFLAGATSFLVGHTLYISEIIHIMF
                     KGQALPWWFYAVVSFGVLLFVLAFYPMSKKISGDRYLTLVGNTYIAVLILVSVVSIIA
                     CVKGFTNFMVLGIIGGISFLASDLILVQATFVKDFKRRDYYIMLFYLLGQALIVSGLV
                     LTYVFAA"
     gene            6043..6261
                     /locus_tag="PLU08_01425"
     CDS             6043..6261
                     /locus_tag="PLU08_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70403.1"
                     /translation="MKKHNLLFITLTVVTLLSGCKKYRSRGSVLSGELLRNNITKVLK
                     KAEPLNVKKIYEQLYLVMMKFITIMTLH"
     gene            6321..6653
                     /locus_tag="PLU08_01430"
     CDS             6321..6653
                     /locus_tag="PLU08_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70404.1"
                     /translation="MRKQIIHNIFKKALVRDIYVTNNEVSGAFIRVYYDELYIFDGFG
                     HLLYQGTQEIHSISSGINNEGKHYVKLYINPVFTTPITYDEVYFYVYDALGNISKVAT
                     DPAPNTQK"
     gene            6672..6890
                     /locus_tag="PLU08_01435"
     CDS             6672..6890
                     /locus_tag="PLU08_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70405.1"
                     /translation="MSEYGLPLYSLVTNEESQEIKDATGKTIVTLKKEVGVYRSQIFA
                     GHYFYQKITKGSKSAINYDLKDIYGNTY"
     gene            6891..7655
                     /locus_tag="PLU08_01440"
     CDS             6891..7655
                     /locus_tag="PLU08_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70406.1"
                     /translation="MSTHSVNLKTGRIKTLKFPYFLAAISPLYDKNNIYNYAVAEIAT
                     FDAFNSKTANYVSEKFIINYKGHLLDQVSDMPLENFVEIQGGFYNKATNKLYDEDVKL
                     IKEFKDFTSSIVLAQQGLIIGTKDGKDGVINARGDEVIPFIYDKIFDSEKEGYMIANK
                     SNMSYRIKLATNEATSLSSVVTKAGNEKTPLYTYFDASRLYPITVLNEKEIVYQFRIE
                     GEVSTYWQPTFMTFTQSAMPGYNVVLKAQVIIDTKI"
     gene            7881..8756
                     /locus_tag="PLU08_01445"
     CDS             7881..8756
                     /locus_tag="PLU08_01445"
                     /inference="COORDINATES: protein motif:HMM:NF021471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HOQ70407.1"
                     /translation="MKKCVFYTALSGLALTIFGLIVAFFGYRLDLFVEAIPHLVGLFP
                     ALWWVILPPAVGLLVLIINIAVKAKKKNKCAIVPFALFVSILAYLLVVPRALPEWLAE
                     GVAPESLVFTTGAAVVVIVFLVLFVLAFVDMALPTRKQLRAEKELDEEVYEVVEEKPT
                     RGPHDAPVLYSDEEVEDEEGLEEVSEAERKVAEKPKAKKAKAKETEKAKEEEEEVKKE
                     DVKKEKEYDRIYHIMKRAKDDRWIVKIAQSRKAIKIFDTQKEAIAYAEILASNNNGVV
                     RVFASKGANKGRIIQ"
     gene            8825..9376
                     /locus_tag="PLU08_01450"
     CDS             8825..9376
                     /locus_tag="PLU08_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70408.1"
                     /translation="MAKIIIISGDLAAGKSVLAKKLSRHYQIPYFSKARFKEILGEKI
                     SFEGIDENKNISRSSYALLRHIGWRVAQVKGSVILEANFHTELLEQIKRDVEFTGHKV
                     ELLYLTGDLDVLFERFLYREHYENRHPVHLTHPLRSLEEFENYILKLRNEPEVLPRKM
                     LDTTNCDQEMIFEKALKLIGSLD"
     gene            9392..11458
                     /locus_tag="PLU08_01455"
     CDS             9392..11458
                     /locus_tag="PLU08_01455"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOQ70409.1"
                     /translation="MSYTTDKIRNIIFLGHQGSGKTTLVEQIASNILKQPAGSVERKN
                     TISDFTPEEKARLTSCSMSVVSLDYNGYRLNLLDAPGNDDFVADVVGVLDFVKAAVLV
                     VDTGKIEVGTVKHFNLLKKYGVPTFIFVNKLDKELVNFDEVLEKITGTLGKKGVSFTY
                     PLNDGKVFNGFVDVTTLKAVKADGSKRVEVELDEATKAKVSEMNNALVEQVALTDDTL
                     LEKFFSGEELSAEEIANGLKMAVLSGEIAPVIVGSSTENIGVNTLVDMALTYLPSPAF
                     IKEMKGKDRGNNEVVRAISENGPFSAYVFKTVYDQYKGTTNYAFVVSGSLELGSDVYN
                     AELNSTMRASQMFSLLGAKQNNIQKAIAGDIVAFAKLDGITTGHTLCDKNDVVTFPRA
                     NYPTIVYRRAISAKNSRDEEKLVQALYRVAAEDPTLEITRVPETKQLLLGGLSDSHLN
                     FVLDKVKNTFGFEANTDAPQVIYRETVKREASAVGRYVKQSGGSGFYGVVEMRFGPSG
                     SDENVFTEEIFGGTVPKNYHPAVEKGFFESVQEGQLAGFPVIGMKATLFDGKYHSVDS
                     NEQAFRMAAILAYREAYMNCAPTLLEPIMKITISVTNEFTGNVMNDLNQRRARVLSMD
                     ESGYGTQEITALVPEAELLDYAIKLRVLSQGSGSFNSEFDSYQEVPTNLLQGVIAANS
                     RLEKDK"
     gene            complement(11521..12474)
                     /locus_tag="PLU08_01460"
     CDS             complement(11521..12474)
                     /locus_tag="PLU08_01460"
                     /inference="COORDINATES: protein motif:HMM:NF023156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HOQ70410.1"
                     /translation="MEKRLRVALYLKMLVGFMISFIIAYLLKMPTSYTAGVIAVLNLW
                     YSRDNVVKTALIRVLSSLIGLATSALLFFLLGYNIFTLFFMVFIVLALLYVFKLEYGA
                     TPTLVLIGQQWAQQTSEAPLNAFVTLLIGVIPALLLNLITFRKSKILLINQQKLDTEI
                     ANIFTYFEKDEIYDFSVIDKILKDTRESLQIALDNYEVKNIMQNFHYINMRTEQINVL
                     KRIVNDLNMLEASPYKKEITNYLIKFKDRIGLEDYASDLLVKHDKLVAYYRALELPKT
                     RDEFEHRAILFAILEEIKIFLILKLDYHKIYPCQKQLETTK"
     gene            12797..14359
                     /locus_tag="PLU08_01465"
     CDS             12797..14359
                     /locus_tag="PLU08_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ70411.1"
                     /translation="MLNLFKTYKGDNVPKLTKYVYSFTGIGRDAAYTLINLFLMIYLQ
                     LTLPESDPQQYKLMIMTIMITMLVIHLWDAVNDPIIGMLIENSRFKLGKYKPWILIGG
                     VLNGLLLMVLFSFRPFGGWGYVIFFIVTYLLWEISFTLNDIGYWGMVPSLTRDPKQRN
                     QITMLMAIAINIGAFTVGGLVPSLYPGRAIQVFRTLSIIIGSVLIFSQLLLVFLCKER
                     DRSEVDSEEIIKVKDIFKVVFQNKPLLVMLGAIVLHYLGGSMFNNLGQNYMHFTFGYA
                     EGGQAFFYFLVGYAVGSIAANFIFGFLVKKYTRAQILRGAFTISAIGNSLLFVIGSTH
                     AFGWLQIPNSIYQWILPVLALIIFTPNGVFYLTLLVQITNTVEYNEWKFGERKEGVVF
                     AARPFAAKISNAITVIVVFLTLTVGGVYDITNKISDYEVLGGLPEGDARRLITEEVQD
                     LAQAAIDGANLGRARFVLILGMALLPLICQTLAYLLVSRKYPIDEKMYDQMVKEIDER
                     RETAAEEQVVEA"
     gene            14523..15851
                     /locus_tag="PLU08_01470"
     CDS             14523..15851
                     /locus_tag="PLU08_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70412.1"
                     /translation="MQKRNKFLSFLALFTFLFGVSVTKTQVARSPQKAEAAEFTVTWN
                     VGEQGYTDGEFAGSKVIDDNFEINFYSGRYLLAPRYFSEGSGYISLQGSGDWLGNGFL
                     ISPRPTINNYFINKIVITATDAAYTSKMRTQVGQTMRILFFRTNEFFSGATYTFEELS
                     KFRYFNLENYAGNEFRIKQISLTYTDVPPAAWVNLTSVTKAKDHYDIKRGVHLLIDKP
                     TIEPSNAFYDYFLTTEDTRLRINGPYVVAEDLCENAVVTINTFGRDAGGERLTTTFTV
                     SVTEAPLTIDGAVGMGYGSIDEKLYRFENVRVQDATLDSDNIVLHASATATNKINVMH
                     PGFNPLNTYTYVKGGTMSLFAKVRRNTTTGKNFLVEPEVISYTDKAILFANTIMASDE
                     VGQCVTKYATVRAIINEMTVEELAKLKNTGVVNGINFVLLERVTSHGHYI"
     gene            complement(16098..16841)
                     /locus_tag="PLU08_01475"
     CDS             complement(16098..16841)
                     /locus_tag="PLU08_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOQ70413.1"
                     /translation="MAEKNLALLIDAENISPDYMSIIRDEANKYGNLTYKRIYGDWAS
                     SNLTKWRSKCIEYNLEQIQQVSYVSGKSTSDFTLVIDAMDILYSDQVDGFVLVSSDSD
                     FTRLVSRLRQNNKFIIGMGESKTPLSLVNSVDLFIYLDKIKNIRDKNVKPKKRGRPAA
                     DKKEEESIISLDDLIDVLTNIISETSDENGWAYWSNTNNTLVRKYPGFDPRNYGFKGK
                     ALQFFLANGFEKRNEGLDVFIRPKANNEQ"
     gene            complement(16857..19175)
                     /locus_tag="PLU08_01480"
     CDS             complement(16857..19175)
                     /locus_tag="PLU08_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ70414.1"
                     /translation="MKHALNYNWSFIPKFNDEYIKSIPQGVISVDLPHNMVDFPFNYF
                     DEKDFDSVGTYFREIVFTKVLESDKFVLRFGGVNNSFTLYVNSQLVGTFTRPYMEENI
                     DITKYVVEGNNLIVVCVNGSEKVNAPPFGNTVDYFPFSGIYREVTLFVYNALDIIDVK
                     VTANHLGQIEVNPDIRNINKVDYSVNYEVYEGTKLIASSIEHNFTVENPKLWSVNNPN
                     LYNLVTTITANKDVKQYVSVFGFRSAVFLSDGFFLNGEYTPLVGLNRHETYPYVGAAA
                     TSFLQFYDVVKLKNLGVNYVRCSHYPPSRAFLDACDFLGLMVINEVPGWQYIGDEAWR
                     KIHLQNIETMINRDFNRPSIIAWSIRINESADHPFYEECHKLAKALDPVRATTGTRNF
                     TNSELFEDIYSYNDFSHDGTNKGLLAKRKVTKSKKPYIVSENNGHMYPTKPFDNPLIL
                     ESHTKRHLAVLDSFFGDKNIVGVSPWCMHDYYTHHEFGSGDKICYHGVLDIYREPKPA
                     AYVYTSNLTSDPVLFPLFRGDNGDLPESKLYKTMVLSNASYIDLYKDDEVVKRYYPRR
                     DLYKHLPHPPFIIDTFISDNFKSTIKFSKRDRKKIAKLLNYAALNNFSRLSLWQKLQL
                     GWLIVKYRLKWDNLVELWFLNVVGWGDKSSGYTLVGYNDEDIEIIRKRIENTTTTTYE
                     IELLKNELVNTDTFDTTIVTIRSLSPYRNHYDFSPVSVVVTGPLKVYGPEIQNLHAGV
                     LNVYVRSINTTGRGTITIGVGGTIKTFNVEIK"
     gene            19251..21581
                     /locus_tag="PLU08_01485"
     CDS             19251..21581
                     /locus_tag="PLU08_01485"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOQ70415.1"
                     /translation="MEYKYIHLKTNDTSYVLAINKYGHLENLYYGAKLALNEDLSILV
                     NKQTNMQGTTTVYSGEPHDTYSLDFVNLELGTIGKGDYRRPSILLEHELGFASDFVVK
                     KHGFAGREVLLVNDLPIPHGYDEYFYVILEDNISKVKVQLDYLIFKRANVIARRLVVL
                     SGKGKLTKAASLNLDLNNDHFDVFSTYGTWANEVHASRNKLVPGLFEVATSVGPSSNR
                     HNPFILLLNEKTDLHKGIGYGVNLIYSGPFKASVELTFDNRVRLQHGINDETFSKELD
                     GKPFITPFAALTFSDKGENGLSQNFHRFVIDHVVRYEYARKPRPILLNNWEATYLTFD
                     EAKIKKLMREASKLGIELMVLDDGWFINRDDDFGGLGNYEVNKKKLPRGLKGLSDYAT
                     KLGMSFGLWFEPEMVSADHPIFTEHPEWVVKIPSVKPSTGRHQYSLNLTLKEVQDYIV
                     ENVSKVLKSADIKYVKWDYNRNLSDYYSPSGEYGTFFYDYTLGLYAIMQRLTKEFPTI
                     LWEGCASGGNRFDLGILSFFDQIWVSDCSDAYERIRMHTNLAKAYPLSVMSGHVSAAP
                     NHQLLRDLPLQTRFNNAFMSGGFGYELDVNNLTPKDKKEIKAYNEIAKAFRSVIMDGK
                     YYELTLSSAKDKKAYALLSEASDNALLLISSGIHSPMPEVLKFPSIPVKQDLTYEVFA
                     LPQSTDISTFGSLLNMVLPKFIKHDGAVIKYLDKRSSAEELVKVEVIERYTVSGKALR
                     AGALTLYPEWTASGFSSTTRMTRDFTSVLYFITKKD"
     gene            21618..23129
                     /locus_tag="PLU08_01490"
     CDS             21618..23129
                     /locus_tag="PLU08_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70416.1"
                     /translation="MVEILVEKLLAYAELKLALNSEDINYYRNVLLNKFGLKKPYKGV
                     INVSEIQSMNNPQMLIDEMQHLIETKGLPAHLSFTDPEEVIIDVLGMMTPIPSVVSQR
                     FAEIHKKDSNKAIKYLYDLSIANNYIQEAKVAENIRWRAELSDKYFEMTINLAKPEID
                     NKETVKLLKEKQDDKYPSCPLCLENVGFGGNESTPPRQNLRAVPLTLDKEEWYLQFSP
                     FVYYKDHIIVISKDHNPMTITPRIFSKLVAFVDQFPDLFIGSNSDIPIVGGSILNHEH
                     FQGGAKVMPMFSAKDRFVLKHKHDRKLKISILEWDSNVIKLESRTKSLLLAKAAEITG
                     NWLEYTNEELGIIPATSGERHAAVTPIVVKEKHKYIMYLILRNNRTDEKYPTGIFHAH
                     PEYHNIKKEGIGLIEQMGTFILPARLKNEFGLIEELYKKGVTGAEMISEHPSIAKHAT
                     FIKELMLSKIKPTEFEKYARDYMTNVCENILQNTACFKNDEAGQDAFIKFLQL"
     gene            23196..24221
                     /locus_tag="PLU08_01495"
     CDS             23196..24221
                     /locus_tag="PLU08_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70417.1"
                     /translation="MGTNSKFNLGINLTGDSDLDRHNIFNYIIENFTKDKTEAEILVL
                     AATFLGKDTKTIFNEIRVNPEVLVPVCLYLMSEEKIALSRRNEIGNALLNYTDAHARK
                     IDDKATRKPLYNFTLEYEKALYTKMANSNVEDILVTFPFYRIYYTQGLIYEAMADYTK
                     AFVMYSKAHQWNPFNPKPILKILEGLKYDGRAQDLLRLSMWYLQITYSAPNISAALRY
                     AGYALYLDGKFAEAYAFYYQAIVYQDGETPARFNEEITSVLTALKKDAPYELSRAQIK
                     KLFAYSKFKPYPSEIAFDTIRPLIINHYTKGNYVDAIRYATQYVIFRPNDDKIMRILK
                     ASKLNLD"
     gene            24331..26448
                     /locus_tag="PLU08_01500"
     CDS             24331..26448
                     /locus_tag="PLU08_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ70418.1"
                     /translation="MNFNPFPNYEQDEKILEKFGRFLDEDAKLNKSDPVIGRDSEIRR
                     IIEILARKQKNNVILLGEPGVGKTAIIEGLAQRIVAGDVPKNLADKRIYELDMGALVA
                     GAKFRGEFEERLKAILKKIKESNHQIILFIDEIHLIVGAGKAEGAIDAGNMLKPMLAR
                     GEIHTIGATTLDEYRTYIEKDRALERRFQPLIVQEPTLEDTISILRGLKARYEAHHGV
                     KISDNALVSAAVLSDRYITERFLPDKALDLVDEACALIRVEVESLPAELDEIRRKMRQ
                     LEIERVALSKENDDLSKVRLQKVNEELENLKKEDEAFSKEWQHELDELNVLGKAKKEL
                     EQARFNLSVAFNSGNYEEASTLQYKVIPELEAKIETLTKETKDNKVLKETVTENEIAA
                     VVSRATGIPLKRIMESQKEKILSLYDELKKRVLGQDVALRLVSDAILRQRAGLNNPNR
                     PIGSFLFLGPTGVGKTEVAKSLAYALFDDERHIVRIDMSEYMEKFSVSRLIGAPPGYV
                     GYDEGGQLTEAVRRSPYSIILFDEIEKAHPEVFNLLLQLLDDGRLTDSQGRVVNFKNT
                     VIIMTSNLGSEFFLKNQTSKIDDLVKATFKPEFLNRIDEIVYFLPLSKDTQRQITRKM
                     LDELMAHVAKNNFKLIYDEKIVDFVLDNAYAPEFGARPLKRFIQKSLETFIAEQVVQG
                     NIDSSITYRLTVTNNEVSISPFA"
     gene            26515..27555
                     /locus_tag="PLU08_01505"
     CDS             26515..27555
                     /locus_tag="PLU08_01505"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOQ70419.1"
                     /translation="MKIRRLLLALIGVTLLASCEPPTSQSIEVPESEIPSVSDISDTP
                     SIVSETSTTEEVTSVEESSEEVSSEWVEPEPVLKPAENWNFRPLPAYTGNFWNTIDFT
                     LRGEELRQALADYMWSKFYVPGVLDNRVTYDDALEAIKVMDADPNKTGNVLSLYDLRS
                     HPHAGYPGKWNREHVFPQSKLADGDKSLRASGNTKNISSDIANLFACDAALNEHKSNH
                     SYIEWNYAEDPERWFPYLSRNTAGQLVDAIVHYGYSPTPIVRGEIARAQLYMVLMYPD
                     RCSISENFQIADMIRWDQEWAPTIERDGQRQKGIEQYQKIRNPFIDNRNLSCYIWGNT
                     NRATRKLCADIY"
     gene            27687..27760
                     /locus_tag="PLU08_01510"
     tRNA            27687..27760
                     /locus_tag="PLU08_01510"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27719..27721,aa:Thr,seq:ggt)
     gene            27837..29195
                     /locus_tag="PLU08_01515"
     CDS             27837..29195
                     /locus_tag="PLU08_01515"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOQ70420.1"
                     /translation="MRITMKEKVKICKMHIEEGLSISHISELYGGYDKTNIKYIINLY
                     KMHGEEPFLSRVRRMYKRDTKLLAIGQVLAGRTIRDVACEHGLANPGVLSDWLKKYRE
                     GGEEAIQDTKARANYLLKEDRFKAVVDRKIEAENERLRAEIAYLKKSQSLAQKLEGAT
                     VQERVRIVDELRTKYKLSILLELSGLSSSVFYYTLNANKTRQNKYVELEKEIERLFIK
                     VHKKRCGYHQIYNNLKKNGWIVGKNKVLEIMRAKGFSKPKVIKWRKYNSYEGNLGLKI
                     ENIMNQDFKTTRPYEKGGTDVTEFPMFGESVYLSPVIDFHTREVLAYSVGTDAKVYRI
                     IRMLRELQKKHGNNLMGMILQSDQGVQYQNSRYIVELRNMGIIQSMSRKGNCLDNSPT
                     ENFFGRLKQEIWDNNKRDYDSVEALITTIHEYINYYNKTRIIMKTKLTPIECRNNYLL
                     SI"
     gene            29755..31101
                     /locus_tag="PLU08_01520"
     CDS             29755..31101
                     /locus_tag="PLU08_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ70421.1"
                     /translation="MIFKRKIYSDLLNWKSEGGKTALLIEGARRVGKSTIVEEFAKQQ
                     YKSYVLIDFSIASDDVKSLFTNYASSLNDFFFFLSSLTGQKLFERETLIIFDEVQLFP
                     AARQLIKHLVKDNRYDYIQTGSLLSLKQNVAEILLPSEERTIKMYPLDFEEFCWATGH
                     NDLIPFLQKRFEERKPAKELHQLAMKLFRQYMLIGGMPQAVAMFLETNDYTRVDRAKR
                     DILQLYRNDIRKYAKNNEYKVISIFDEIPNQLTKHEKKFTLSALSKDARFRDYEEAFV
                     WLNEAMMTNPCFNASDPTVGLGLYRESLTLKMYMGDTGLLFSHAFNENEIMQTEVAQS
                     IVAGKLNINSGMFLENIVAQMLVASNKRLYFYSRNDRKNAKNTMEIDFMISDPHNPRK
                     ISLVEVKSGKNYTTSSLKKMKVKFGNKLGQSYLLHTKDLRVHEGVIYLPVYMTIFL"
     gene            31419..31883
                     /locus_tag="PLU08_01525"
     CDS             31419..31883
                     /locus_tag="PLU08_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOQ70422.1"
                     /translation="MTNEHFAIVYETYKVMVFQIAYSYLRNVEDAEDATLESFMRLLR
                     INRKFASGDDERRYLTRIVVNVSKDMLRKKRPVPISDPVIAVNDAGQNENNYIWDEVN
                     KLPNKYKDVIILRYINEHSFNEIASVLKISASAARKRHERALKMLKEELSHE"
     gene            31876..32640
                     /locus_tag="PLU08_01530"
     CDS             31876..32640
                     /locus_tag="PLU08_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70423.1"
                     /translation="MNNHEEKLKKLKNDIPEYPDGLDQKVFRAAEQAPRYKRPGFPKL
                     LIPSLLTVTAVVLSTFFVTKYVYGWNTPSNTPSNSGMPSEPSNTSAEEGEVYDYYVSA
                     SAAIPGASLAPEMLVEGKLGYFDIVYIDSQLALDATINQYADDAHPVLSTLTSVIRNN
                     AEFFSESVLIIVPFAHTSTEEGIELTQIEFGSKEQPTKLRFDIYSPEFNDMDTLVTFH
                     LVTIGEWASDLLSESAFKISVHNTFDNSNKSVFYSN"
     gene            32651..33679
                     /locus_tag="PLU08_01535"
     CDS             32651..33679
                     /locus_tag="PLU08_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70424.1"
                     /translation="MKRTQILVAGLLLILTSCRAANHQTLSEVFPMLKTEEFAEVKHT
                     ISCNSLAPLALINHYYNDDVAAVTNFVNELGKTKVRRYAKLPESKNVACPTINTYAFK
                     NEALEFTFDVTNMFVVKDDVYYELEELPNIVTTYSSYSFSGSTTITEVFNSEGKEITD
                     FSLSLPSLEFKELTELSGNNVNFTIETVGGIISVYDAVTFGLNVGEQIERFYEVISGD
                     TFANLFDTNNNFTDDTMLLANIPQQLTFRNQKYKMITSGFFEWQDIQLDLLIAYFVNE
                     RDYDVFYLENPDIEYCIDEANTLYYAAGPSKFPLYSLVGHAIEQLLGLLLPQGGGSPM
                     IFLNISCV"
     gene            33739..34389
                     /locus_tag="PLU08_01540"
     CDS             33739..34389
                     /locus_tag="PLU08_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70425.1"
                     /translation="MKKLLTSFLLGLLLLAGCENTRKYRFTIIDTDRTVLSKHKDRYS
                     AGEAIRIETVMLTDVIVSMYVNDEFYNNATTAENNVGQSVFVYDFIMPTYDVTITFVI
                     KDGMIVEPNYIDITFIQRKIVDWQNFEDYLIPVFGEEDLTITTLSFEDNIKLTLEEID
                     DLQSRSVLNYIPPETMYGYYIFYGFYFDEENTNDLHRLTPEFAVTLYNTYYYGLAG"
BASE COUNT        11658 a         5552 c         6748 g        10814 t
ORIGIN      
        1 tggttaatta caaaaggaaa atcaagtgaa cgtattggta aagttcactt gattgttgga
       61 aagatatttc aagaattgaa acatttgtaa catcagataa tacgattagt tatggatatt
      121 ttattttaat aactaatgat ttgagtttaa agaaggaacc attagacact tatttatata
      181 gagaatttag tattgctgaa accacccaca aaagtgggga aagaaaatgg ggtgaaggta
      241 tgtctaagaa ctctaaaaaa ggtcgagaaa atccaatttg tattaaaaat aattatgaat
      301 ttaaatatga agaatttaag gaatataaag gaaaaaatgg cagattcgaa ttacttgtcg
      361 ttgaaattca atagcagttg tatttaatta tctaataatg atgtaataaa atattaagtg
      421 atatcgctaa tattaatagt attgctctaa atataatcga aatgaataaa attgctgagt
      481 aatataataa ataaataaat aattattaat gtgcatcttt aacaatgaat attaatttat
      541 agttttatgc attttatgac aataatcaaa aagcgtcacc actggtgaca caattagatt
      601 tgcctaacaa ttgactaaaa ttataatgtg acttttaaca catgaataaa ggtgatgaag
      661 cgcaaaaatc atacgaataa ttctcaatat tttgcaaaat gcataggaat aatttaaaaa
      721 taactgcaaa aaccatagga ataatttaaa aataattgca aaaaggatag gaatgtttta
      781 aaattgaggc aggagatgta ctttatgaaa cgggaaatta tgcaagatct tatttcttgg
      841 aaaaataaga gaaatagaaa accactctta ttaactggag ttaggcagtg cggaaaaact
      901 tatattttaa gaaaatttgg tgaagagcat ttcgataact atgtctattt aaatcttgaa
      961 agagatccac aaattgctgc cgtctttaat tatgattatg atacaaagag aattttacaa
     1021 gatataagca atttaagttt attacctgaa atcatcccta ataaaacgct cttaattatt
     1081 gatgaaattc aatttaatcc taaagcgata accgcactta aatattttgc ggaagatatg
     1141 cctgaattat gcatagtggg agcgggttct ctactcgggg tatcgcttag agaagaaggg
     1201 gcgtcctttc ctgtaggtaa agtggaccgt ttagaaatgt atccaatgag ttttaaagaa
     1261 ttcttgcttg aatcgaaaag taggagaata gtaaatagta tcgagaactt cgatataaca
     1321 aaactcttgc cagattacat tttggacaat ttgaataggg agtataccaa ttatctttta
     1381 gtgggtggta tgcctgaagc tgttaaagtg tggtttgaaa atcataattt agcggaagtt
     1441 aatgaaatac aagacaatat aatcgcaggt tatgaaaacg atttctcgaa gcacagtcca
     1501 aagaatgaat taactaatat agaacttatt tggcggtcta tccctgagca gcttgcagaa
     1561 gataacaata aatttgtatt ttcaagagta aaaaaatcag cgagagctaa agatttggaa
     1621 aaatcaatcg gttggcttat cgatgctgga cttattcgtg tagttactaa agtcgataat
     1681 ccgcaagttc ctttatcgtt ttatagtaat tcctcgtttt acaagattta tttgtgcgat
     1741 gtcggactct tatcgagaat ggctggtttt acgcttaaat cgttactcga aaaagatgag
     1801 tcaacaggtg gatttagggg ctctttgagt gaaaacttta taaataatga gctagtttca
     1861 ttaggatatg tgacttactt ttggcgatca aaaaatagtg cagaagtcga ctttctaatt
     1921 gaggatgatg ggagagttat tccaatagaa gtaaaggcaa atgtcaatac aaaggctaaa
     1981 agttacaata attttataaa aaaatatgca accaaaattg gttttagatt ttcactaaaa
     2041 aatagcggtg aaaataaagt tggagtcaca aaaacttata gtttgccgtt atctttagta
     2101 tttagattaa aagactatct gaactaatat aatttcccgc ggaaaaacta caactactgg
     2161 aataaaattc catcaatcac tggaatacag tagtgattac aagtttaaaa gtgaggttat
     2221 tcttaactct ttagtgcata aaatatcttt ttaatcaata attaaaagaa atagaagggg
     2281 tagtagtttt atgagtaaaa ttacacatgt caaaaacttt gtgggtatct attcaattgt
     2341 ttttgctgta ttaggctttg tggctttaat catcgataaa acccatgaac cgattattca
     2401 tattttatta acactctttt ttatccttgg tgttattgtc catgtcccgt ttttattaaa
     2461 gattcattta gtgtcaatga ttgtgatggg gtttctacat ggttcattac ttatcatgag
     2521 tatcattgtt atgtttcatg actgtttaac gggtttattt ggttttggtt tatttattct
     2581 tcctttagtg gttagcattg tttattttgt tatgtacagt aacgataaga aaaagcaaaa
     2641 gaatttatta gtagctgcat tagatagtaa taacacaaaa gaatctaaca ctttaaatga
     2701 tgaattaaat gcgcttttag catctaaaaa caggggcgaa ataaccgaag aagaatatct
     2761 tgcaaaaaag aaagatttaa ttggttatta agatttaatt ttattcaaat ctaatttacc
     2821 taacgcatta catttatcaa ctaaatagcg tgccatctct aagcgggtgg gcgctatttt
     2881 tttaagttca ctatatagaa ttggtttatc aaatagataa actttttctt ttattttaaa
     2941 gtatgatgca tataagtatg tatcaatacc gtgcttataa gcaatttctg ctagaatgca
     3001 aaaaccacca tgaaatcctt ttaattcatc taaatatcct tcgtcactaa cttcggggaa
     3061 aataacgata ttgtcaccat ctttaaaagc ttctaagctt tctttaactg tctttttaag
     3121 tcgtaagtct tgataagttg aaattagtcg tagtccttta tagaagaggt tagttaaagg
     3181 acttgctaaa agacaaaaaa gtcttgctaa atgtaagttc cagccctttt tctcatgata
     3241 atacactagt gactggtaac gatacatttt acgcagccct gaattcattt catgtgtccc
     3301 ccacatccgt aagtggaatg gagcgtaaat ttcaagtgac atcggcgcat cagtgccgac
     3361 atgattactt aagataattg catttgtcgt tggtttttca ccaagaaaga taaactttgg
     3421 ttctttataa cggactttga tgatacgttt taaagcgcgg taccagcgct tacgtttttg
     3481 tttaaattta ataggtttag cttttttcat aagtaccccc acaagaaaat attaacatat
     3541 attcaataca atattatttt tttatatgtt tttgacatta tctgtcaata ttcctcatgt
     3601 aagtgttttc attttcagtg tcatttttta caattttgaa attatgctaa tgacactaaa
     3661 ttaattttaa aagttgttaa attatgtcta tctttttaat agttaagcgt ttaaaaagtt
     3721 gtgttaaatt tgttaagatt attaatgaaa atatttgaat agagaaaagg ggtaagatta
     3781 tgtttcaaaa ttttgatggt ggctatattg aagtcgtatg tggcccgatg tttgccggta
     3841 aaaccgaaga attaattaga agaattaaac gcttagaata tgcgaaacaa tcatatatta
     3901 tttttaaacc aaagattgat ggtcgctatg gcgataatgt gattcaaagt cacaataata
     3961 atcgtcgtga agcagttaat attgaaaaag cacgcgaaat ttatacgcat ttaaaagata
     4021 aaacagtcga agttattatc attgacgaag cacaattttt tgatgaagaa ctgcctgatg
     4081 ttgttgatga cctcgctgat caaggctacc gtgtgattgt cggtgggtta gaccggaact
     4141 ttagaggtga accatttggc acgatgccgg agattcttgc ccttgctgaa gtcgtcacta
     4201 aactaacggc gatatgcccg gtaagcggaa aaccagcgac gcgcacccag cggataatag
     4261 acggtaaacc tgcgcattac aatgacccac ttgtgttaat tgatgcggaa gaatcttatg
     4321 aaccgcgtag cagggcacaa catattgtgc cgggcaaaaa gaaaaagtag cgaggtgcta
     4381 cttttttctt aaatagtatt ttaattattt aaaaaatagt cctaaaataa aatcttgaac
     4441 tttaacggaa agagcattta ataatttcat cttaaacgaa ttatttactt tataaacagt
     4501 ttttggtctt ttcttatata ttgcttttaa aaatgtttta gtgattttac gggggtcttt
     4561 agctctttct aactcggtaa gcatcatctt ttgcattttt gtaagcgggc ttttaaagtc
     4621 tttagtgtag gcaaatactt tttcaaattt atgcgtaaca ttaatttgca tatctgtttt
     4681 aaaagcaccg ggtctaactt taataacctt gatgccaaaa cttcgtaatt tctcgtcgta
     4741 aactatcgtt atatgcttca atcctgcgtt ttgctatcgt gtagaaggag tggaagggta
     4801 gtgctaaaag tgtgccgtac tcactactta aatgaataat gcgaccttta gcttttttaa
     4861 gagcgggaag gctttttggt taatgaagta gaccgcaaaa agattaactt gcatcaaggt
     4921 ttcaagtgct tcaaccttta cttccattat tggaccaatc tctaaaatac cggcgaagtg
     4981 aatgactaaa tcaagcttgt ctgtaatttt aagtatctta tcaaaaagat tatttattga
     5041 gtctttatct gttaagtcgc taaccacaat aagtttattt ggtaaatcat tatacttatc
     5101 acttattgct ggattaatat cagtgagtat taaaaagaag tcatctttga tggcagtgat
     5161 aatgttgtgc gctaagccac cactagcgcc agtaataagc gcatatttca taaatttatt
     5221 ttaacactct aaatgtaaat gagtgtcaat atttggaaaa tagcgctaaa ttcactactt
     5281 tttaataata ttaaaagatg ataatataaa agggtaggag aaatatagtt atgaacgttt
     5341 ttgtgtatat cttttttggt ttattcattg cggtgacaat tgctgaacta attatggctt
     5401 ttaatgagat tgaaaaagcg cggatgattc ttaaaccttt ttcaatgttc tttttagcaa
     5461 ttgcggcact tattgctgta cctaatcacc cgcttattta tcttggagca tttttaggga
     5521 tggttggtga tatcttctta ctgtggccta aaaataagaa gatgtttctt gcaggggcaa
     5581 caagcttttt agttggacat acattatata ttagtgaaat cattcacatt atgtttaaag
     5641 gtcaagcttt accgtggtgg ttttacgctg tggtgagttt tggcgtgttg ctctttgtgt
     5701 tagcgtttta tccaatgtcc aaaaagatta gtggtgaccg gtatttaaca cttgttggta
     5761 atacatatat tgctgtttta attttagttt ctgttgtgag cattattgct tgtgttaagg
     5821 ggtttactaa ctttatggta ctaggtatca tcggtgggat tagcttctta gcgagtgatt
     5881 taatcttagt acaggcaact tttgtcaaag attttaaacg ccgcgattat tacattatgc
     5941 ttttctattt acttgggcaa gcattaattg tttctggatt agtgctcact tatgtctttg
     6001 cggcctaatt tctaatatcg atattggtaa aggaggaact tagtgaaaaa acataattta
     6061 ctttttatta ctttaaccgt tgtgacttta ctttcaggat gtaagaagta taggtcgcgt
     6121 ggtagtgtgt taagcgggga gttactgcgt aacaacatta caaaagtgct aaaaaaagca
     6181 gaaccgttaa atgttaaaaa aatatatgaa caattgtatt tagtcatgat gaaatttatc
     6241 accattatga cactgcacta attagagata taattgcgac gaaaaatggt gatgattgta
     6301 ttggttttta ctcattagcg atgcgtaaac aaatcattca taatatattt aaaaaagcct
     6361 tagtgcgtga tatttatgtt actaataatg aagtatcagg cgcctttatt cgtgtgtatt
     6421 acgacgaact ttatattttt gatggctttg gtcatttact ctatcaaggc acacaagaga
     6481 ttcatagtat aagttcaggg ataaataatg aaggaaaaca ttatgtaaaa ctatatatta
     6541 atcccgtatt cactactcca attacttatg acgaagttta tttttatgtg tatgacgccc
     6601 ttggtaatat atcaaaagtt gcgactgatc cagcgccaaa tacacaaaaa taaagtagta
     6661 acgcggaaga tttgtcagag tacggattac ctctttactc gctagttaca aatgaagaga
     6721 gtcaagaaat taaggacgct actggtaaaa cgatagtgac gctaaaaaaa gaagtaggcg
     6781 tttatcgtag ccaaatcttt gcgggtcatt atttctatca aaagatcaca aaaggaagca
     6841 aaagtgcgat taattacgat ttaaaagata tttatggcaa cacttattaa gtgtcaacac
     6901 atagtgttaa tttaaaaaca ggtcgcatta aaacgcttaa gttcccctac tttttagccg
     6961 cgataagtcc actctatgat aaaaataaca tttataatta tgctgttgct gagattgcta
     7021 cttttgatgc ttttaatagc aaaacagcaa attatgtttc cgagaaattt attattaatt
     7081 ataaaggtca cttgcttgat caagtaagtg atatgccgct tgaaaatttc gtggaaattc
     7141 agggcggatt ttataataaa gctacgaata aactttatga tgaagacgtt aaattaatta
     7201 aagaatttaa agattttaca agtagtatag ttctagcaca acaaggactt attatcggta
     7261 caaaagacgg taaagatgga gtgattaatg cgcgtggtga cgaagtaatt ccatttatct
     7321 acgacaaaat ttttgatagt gaaaaagaag gttacatgat tgctaataaa agtaatatgt
     7381 cgtatcgcat aaagttagcc actaatgaag ccactagttt aagcagtgta gtcactaaag
     7441 caggtaatga aaagacaccg ctttatacat attttgatgc aagtcgcctc tacccgatta
     7501 ctgtattaaa tgaaaaagaa atagtgtatc aatttagaat tgaaggtgaa gttagtactt
     7561 actggcaacc gacttttatg acatttacac aaagtgcaat gccgggatat aatgttgtgc
     7621 taaaagccca agttataatt gatactaaaa tttaatatta ttttgttcac tttgaatatg
     7681 tagcgcacgc ctataaaaat atggatactt atggcagtga agttttacaa ccttaaaagg
     7741 aaagtaaaga ataaagcgct tactacttta aataaaagca aaatggggca aatattcggt
     7801 attttagccc ctttttgttt acttctgagt attattttta tataatacta ttgagtttta
     7861 tattaggaaa aggagaactt atgaagaaat gtgtttttta taccgctctt agtggtctcg
     7921 ccttaacaat ctttggtctc atcgtggcct tttttggtta tcggcttgat ctctttgtag
     7981 aggcaattcc ccatttagtt ggattatttc ccgcattatg gtgggtaatt ttaccgccag
     8041 ccgtgggact tttggtgtta attattaaca tcgctgttaa agccaagaag aaaaataaat
     8101 gtgcaatcgt tccgtttgcg ctatttgtgt ctattcttgc ttacttactc gtagtgccac
     8161 gcgcattacc agaatggtta gcagaaggcg tagcaccaga aagtttagtg tttacgactg
     8221 gtgcagcagt tgtcgtcatt gtatttttag tgctctttgt cttagcgttc gttgatatgg
     8281 cactcccaac acggaaacaa cttcgcgctg aaaaagaact tgatgaagaa gtttatgaag
     8341 tagttgaaga aaagccaaca cgcggaccac atgacgctcc agtactatac agtgatgaag
     8401 aagtcgaaga cgaagaaggc ttagaagaag ttagcgaagc tgaacgcaaa gtcgcagaaa
     8461 aaccaaaagc gaaaaaagca aaagcaaaag aaacagaaaa agcaaaggaa gaggaagaag
     8521 aagtgaaaaa agaagacgta aagaaagaaa aagaatacga ccgcatttat cacatcatga
     8581 aacgggcgaa agatgatcgt tggattgtta aaattgccca aagccgcaaa gcaattaaaa
     8641 tctttgatac acaaaaagaa gcgattgcat atgctgaaat tttagcgagt aacaacaacg
     8701 gagttgttcg tgtcttcgcc tcaaaaggcg ccaataaagg gcgcattatt cagtagtaaa
     8761 aagaaggcac ttaacacaag gtgccttttt atatattgtg ctaagtagaa aggaatatta
     8821 aagcatggcc aaaatcataa ttatttcagg tgacctcgcc gctggtaaaa gtgtgttagc
     8881 aaaaaagctt tcacgtcatt atcagatccc ttatttctca aaagctcggt ttaaagaaat
     8941 actcggcgaa aaaatttcgt ttgaaggaat cgacgaaaat aaaaatattt cgcgctcctc
     9001 atatgcgctc ttacgccata ttgggtggcg ggtagcccaa gtaaagggca gtgttatttt
     9061 agaagctaat tttcacactg aactcttaga acaaattaaa cgtgacgttg aatttactgg
     9121 tcacaaagtt gagttgctct atttaacggg tgatttagat gtactatttg aaagattttt
     9181 atatcgtgaa cattacgaaa atcgccatcc agtgcactta actcatccac tacgttcatt
     9241 agaagaattt gaaaattata ttttaaaatt acgtaatgaa cctgaagtat taccacgcaa
     9301 gatgcttgat accactaatt gtgatcaaga aatgattttt gaaaaagcac tcaaactcat
     9361 tggtagttta gattaatatg aaggagaaat tatgagttat accacagata aaattagaaa
     9421 cattatcttc cttggccacc agggctcagg aaaaacgaca cttgtggaac aaatcgcaag
     9481 taatatctta aaacagcccg ctggcagtgt tgaacggaaa aataccatta gtgactttac
     9541 acctgaagaa aaagcgcgtt taacatcatg ttcaatgtcg gttgttagcc ttgattataa
     9601 tggctatcgc ttaaacttac ttgatgcacc aggcaatgat gactttgttg ctgatgttgt
     9661 tggtgtgctt gactttgtta aagcagcagt cttagtcgtc gatactggta agattgaagt
     9721 cggaacagtt aaacacttta atttattaaa gaagtatggc gttcctactt ttatttttgt
     9781 taataagtta gacaaagaat tagttaactt tgatgaagtt cttgaaaaaa ttactggtac
     9841 actcggtaaa aaaggcgtaa gcttcactta tccgcttaat gacggtaaag tatttaacgg
     9901 ttttgtcgac gtcacaacgc ttaaagctgt taaggcagat gggtcaaaac gcgttgaagt
     9961 cgaacttgat gaagcgacta aagcgaaagt cagtgaaatg aataatgcgc ttgttgaaca
    10021 agtagcgtta acggatgaca cccttttaga aaaattcttt agcggtgaag agttaagcgc
    10081 tgaagaaatc gctaacggac ttaaaatggc cgtgttaagc ggtgaaattg ctccagttat
    10141 tgttggtagc agtaccgaaa atatcggtgt taatacactt gttgatatgg cacttactta
    10201 cttaccaagc ccagctttca ttaaagagat gaaaggcaag gatcgcggta ataatgaagt
    10261 tgtgcgtgca attagtgaaa atggaccttt tagtgcttat gtctttaaaa cagtatatga
    10321 ccaatataaa ggcactacta actatgcctt tgtcgtaagt ggtagtttag aacttggtag
    10381 tgatgtgtat aatgctgaac ttaattccac gatgcgtgct tcacaaatgt ttagtctttt
    10441 aggcgctaaa caaaataaca ttcaaaaagc gattgctggt gatatcgttg cttttgcaaa
    10501 gttagacggg attaccactg gtcacacatt atgtgataaa aatgatgtcg ttacattccc
    10561 gcgcgctaat tatccgacga ttgtttatcg ccgcgctatc agtgcgaaaa atagtcgtga
    10621 cgaagaaaag ttagtgcaag cactctaccg tgttgccgca gaagacccaa cccttgaaat
    10681 tacgcgtgtt ccagaaacaa aacaactctt acttggtgga cttagtgata gtcatcttaa
    10741 ctttgtgctt gataaagtaa aaaatacatt tggctttgaa gcaaataccg atgcaccaca
    10801 agtaatttac cgtgaaacgg taaaacgcga agctagtgca gttggccgtt atgttaagca
    10861 atcaggcggc agcggcttct acggagttgt tgaaatgcgc tttggcccaa gtggtagtga
    10921 tgaaaatgtc tttaccgaag aaatcttcgg gggaacagta ccaaagaact atcaccctgc
    10981 tgtcgaaaaa ggattctttg aaagtgtgca agaaggtcaa cttgcgggct tccccgtaat
    11041 cgggatgaaa gcaacattat ttgacggtaa ataccacagc gttgatagta acgaacaagc
    11101 gttccgcatg gccgctattt tagcctatcg cgaagcttat atgaattgtg ccccgacctt
    11161 acttgaaccg attatgaaaa ttaccatcag tgtcacaaat gaatttacgg gtaacgttat
    11221 gaatgacctt aaccaacgtc gggcacgggt attaagtatg gatgaaagtg gttatggtac
    11281 ccaagaaatt accgccttag tgcctgaagc agaactatta gactacgcca ttaaacttcg
    11341 ggtcttaagc caaggcagtg gtagtttcaa tagtgaattt gatagctatc aagaagtacc
    11401 cacaaactta ttacaaggtg ttatcgctgc caattcacgg cttgaaaaag ataaataatt
    11461 agatagttta cataaagaaa accatcggtg tgcgccgatg gttttttgta tataatcgct
    11521 ttattttgta gtttctagtt gcttttgaca cggataaatt ttgtgataat caagtttaag
    11581 aattaagaag attttaattt cttctaatat cgcaaataat atagcgcggt gttcaaattc
    11641 gtctctagtt tttggtagtt ctagtgcgcg gtaataggcc actagtttat cgtgcttaac
    11701 gagtaaatca cttgcataat cttcaagtcc aatgcggtct ttgaacttaa ttaaataatt
    11761 agtgatttct tttttatatg gtgaagcttc taacatattt aaatcgttta ctattcgctt
    11821 taatacatta atttgttcgg tgcgcatatt aatataatgg aagttttgca tgatgttttt
    11881 tacttcataa ttatctagcg ctatttgcag actttcacgt gtatctttga gtattttatc
    11941 aatcacgctg aagtcataaa tttcatcttt ttcaaagtag gtaaatatat ttgcaatttc
    12001 tgtatctagt ttttgctgat taataagtaa gatctttgat ttacggaaag taattaagtt
    12061 taaaagtaaa gcagggataa caccgattaa aagcgtgaca aaggcattta atggcgcttc
    12121 tgaagtttgt tgcgcccatt gttgcccaat taagacaagg gttggggttg cgccgtattc
    12181 aagtttaaaa acatagagga gcgctaaaac gatgaatacc ataaagaaga gcgtaaaaat
    12241 gttatagcct aataaaaaga agagcagtgc actagtggca agcccaatta aacttgataa
    12301 tacgcgaata agggcagttt tgacaacatt atcacggcta taccataagt ttaaaacagc
    12361 aataacacca gcagtgtagc ttgtcggcat cttaagtaaa taggcaatga taaaactaat
    12421 cataaagccc actagcattt taagataaag cgcaactcgt aatctttttt ccatacatta
    12481 gatataataa caaaaagttt agtatagtaa ctctttaaag cattagtaag ataatagttt
    12541 gtaagcaata tataacttaa aaagtaataa aattatgttg atttgctcca taaagtgtta
    12601 tttaaggcaa ggaagtggag taaaaaaaca tttgccgaca cttgatgtaa agaagagaca
    12661 aagaatcttt tatataatag aaagcaagta ttaatgaaca taatttgacg atatttacta
    12721 aataacgaac ataatatcaa aataatgttg tatcattaaa atgtatatac tcacataaat
    12781 aagaaaggaa tcatatatgc tgaacttatt taaaacttat aagggagata atgtcccaaa
    12841 attgacgaaa tatgtctatt cttttacggg gattggacgt gatgcagcgt atacattaat
    12901 taatttattc ttaatgattt acttgcaatt aactttacca gaaagtgatc ctcaacaata
    12961 caagttgatg attatgacaa tcatgattac gatgttggtc attcacttgt gggatgcggt
    13021 taatgaccca attattggga tgttaattga aaattcacgc tttaaactcg gtaaatataa
    13081 accgtggatc ttaattggtg gggtactaaa cggtttgtta ctcatggtgc tctttagttt
    13141 tcgaccattt ggtggttggg gctatgttat tttctttatt gttacttatc tcttatggga
    13201 aattagcttt acgcttaatg atattgggta ttgggggatg gttcctagtc ttacccgtga
    13261 cccaaagcaa cgtaatcaaa ttacgatgct gatggctatt gcgattaata ttggtgcttt
    13321 taccgttggc ggactcgttc caagtttata cccaggacgc gcgattcaag tatttagaac
    13381 acttagtatt attattggta gtgtattaat tttctcccaa ttattacttg tgttcttatg
    13441 taaagagcga gatcgtagtg aagttgatag cgaagaaatt attaaagtta aagacatctt
    13501 taaagttgtc ttccaaaata aaccgctttt agtaatgctt ggggcaattg tccttcatta
    13561 cttaggtggt tcaatgttta ataaccttgg tcaaaactat atgcacttta cttttggcta
    13621 cgctgaaggg ggccaagctt tcttttattt cttagtgggg tatgctgttg ggtcaattgc
    13681 cgcgaacttt atttttggct tccttgtgaa aaaatataca agagcacaaa tcttacgcgg
    13741 cgccttcact attagcgcaa ttggtaactc actgctcttt gtgattggaa gtacacatgc
    13801 ttttggttgg ttacaaattc caaatagcat ctaccaatgg attttacctg ttttagcgct
    13861 cattattttt acgccaaacg gtgtgtttta tcttacactg ctcgtgcaaa ttacaaatac
    13921 ggttgaatat aatgaatgga aatttggcga acggaaagaa ggtgttgtct ttgccgcgcg
    13981 tccttttgcc gcgaagatta gtaacgcaat caccgttatt gttgtgtttt taacactcac
    14041 tgtcggcggt gtttacgata ttactaataa aattagtgat tacgaagtct taggcgggtt
    14101 accagaagga gacgcacggc gacttatcac tgaagaagtg caagacctcg cccaagccgc
    14161 aattgacggc gcaaatttag gcagagcacg ctttgtgtta atcttaggga tggcactttt
    14221 accacttatt tgtcaaacac ttgcctatct ccttgtcagt cgtaaatacc caattgatga
    14281 aaagatgtac gaccaaatgg tcaaagaaat cgacgaacgg cgtgaaaccg cagcggaaga
    14341 acaagtcgtt gaagcataag cgttaattag aattaccgaa aagtccaagt tatcaagact
    14401 tgggcttttt tgttaaaaat tcaatctttc accataaatt gttagttatt ttgtactttt
    14461 ttgttgtgag gacttatttt aagttattaa ataaatgagt atattataga taggagctta
    14521 ttatgcaaaa aagaaataaa tttttaagtt ttttagcact ttttactttt ctttttggtg
    14581 ttagtgttac taaaacacaa gtggcacgta gcccacaaaa agctgaggcc gctgaattta
    14641 ctgtcacttg gaatgtaggt gaacaaggtt acacggatgg tgagtttgca ggttcaaaag
    14701 taattgacga taattttgaa attaactttt attctggccg gtatttactt gcaccgcgtt
    14761 attttagtga gggcagcggt tatattagtt tacaaggttc aggtgactgg cttggtaatg
    14821 ggtttttaat tagcccgcga ccgacaatta ataattattt cataaataaa attgttatca
    14881 ccgccactga tgcagcttat acgagtaaaa tgcgtacaca agttggtcaa actatgagga
    14941 ttcttttttt ccggactaat gaattttttt caggtgccac atatacattt gaagagctaa
    15001 gtaaatttag atattttaac cttgaaaatt atgcgggtaa tgaattccgg attaaacaaa
    15061 tttcattaac ttacactgat gttccaccag ctgcttgggt aaatcttact agtgttacta
    15121 aagctaagga tcattatgat attaagcgtg gagtgcacct tttaattgat aaacccacta
    15181 ttgaacctag taatgcgttc tacgattatt ttttaacaac ggaagataca cgtttacgga
    15241 taaatggccc gtatgttgtc gccgaagacc tttgtgaaaa tgcggtcgtg acaattaata
    15301 cttttgggcg cgatgcaggt ggtgagcgcc taacaacaac attcactgtt agtgtaactg
    15361 aagcaccact gactattgat ggtgcggttg gcatgggtta cggaagtatt gatgaaaagc
    15421 tctatcgctt tgaaaatgtt cgcgtccaag atgctactct tgatagcgat aatatcgttt
    15481 tacacgctag tgctaccgca acgaataaaa taaatgtcat gcaccctggc tttaacccac
    15541 ttaacactta tacctatgta aaaggcggga cgatgagtct ctttgcgaaa gtgcggagaa
    15601 atacaacgac aggtaaaaac ttcttagtgg aaccagaagt tattagttac acagacaagg
    15661 ctattttgtt tgcaaataca ataatggcaa gtgatgaagt cggtcaatgt gtaacaaaat
    15721 acgccacagt acgggcaatt attaatgaaa tgacagtcga agaactggca aaattaaaaa
    15781 atacaggtgt agttaacgga attaattttg tcctgttaga gcgcgttacg tcgcatgggc
    15841 attacattta ggtgaaagcg atccttatgg cgacggcacc ccgatttcag gaatgccttt
    15901 agtgccagaa aattcaacta cacaaaataa caatgtcctt ttagttattt tactttcttt
    15961 cagtgttgcg gtgtttctat atttcttaac atcaaaacga aagaaacttt atcttaaaaa
    16021 ataggaatta tttgtagttt actaacaaaa aaactcggtg attacgtatc actgagcttt
    16081 tcttttacaa ataataatta ctgttcgtta tttgctttag ggcgaataaa gacatctaag
    16141 ccttcattgc gtttttcaaa gccgtttgct aagaaaaatt gtaaagcttt acctttaaag
    16201 ccgtagttac gagggtcaaa accggggtac ttacgaacaa gcgtattgtt tgtattgctc
    16261 cagtatgccc aaccattttc atcacttgtt tcactgataa tgttcgttaa aacatcgatt
    16321 aagtcatcaa gactaatgat gctttcttcc tctttcttat cagcagcggg acggccacgt
    16381 tttttcggtt taacgttttt gtcgcgaatg tttttaattt tatcgagata tataaataag
    16441 tcgacggaat ttacaagtga taagggggtc ttgctttcac ccatcccaat aataaactta
    16501 ttgttttggc gtaagcgact aacaagtctt gtaaagtcac tatcactact gacaaggaca
    16561 aagccatcaa cttggtcact atataaaatg tccattgcat caattacgag tgtaaaatca
    16621 ctagtgcttt taccactgac gtaagagact tgttgaattt gctctaagtt atattcaata
    16681 catttactgc gccatttggt cagattgcta cttgcccaat cgccataaat gcgcttataa
    16741 gttagattgc catatttatt agcttcgtca cgaataattg acatataatc aggtgatata
    16801 ttctcggcat caattaaaag tgcaagattt ttttcggcca ttactgcttc tcctttctat
    16861 ttaatttcga cgttgaaggt tttaatcgta ccgccaacac caatagttat tgtaccacgc
    16921 cccgtcgtgt taatggagcg gacatacaca ttaagtacac cagcgtgtaa gttttggatt
    16981 tctggcccgt aaacctttag gggaccagta acgacaactg aaacaggact aaagtcataa
    17041 tgattacgat aaggacttag actacggatt gtaacaattg ttgtatcaaa tgtatctgtg
    17101 ttcactaatt cattttttaa aagttcgatt tcataagtcg tagtagtggt gttttcgatt
    17161 ctttttctaa ttatttcgat atcttcatcg ttatagccca ctagtgtgta gccgcttgac
    17221 ttatcacccc agccgacaac attaaggaac cataattcga caagattatc ccatttaagg
    17281 cgatacttaa caattaacca cccgagttgt aacttttgcc agagtgagag ccgactaaaa
    17341 ttattgagag cggcataatt taatagtttt gcgatctttt tacgatcacg ctttgaaaat
    17401 ttaattgttg acttaaagtt atcactaata aaagtatcga tgataaatgg tggatgcggt
    17461 aaatgtttat ataagtcacg ccgcgggtag tagcgcttta caacttcatc gtctttgtat
    17521 aaatcaatgt aacttgcgtt acttagcacc atcgttttat ataatttact ttctggtaag
    17581 tcaccgttat caccgcgaaa aagcgggaat aaaacagggt cacttgtaag gttagaagta
    17641 tacacatacg ccgcgggttt aggttcacga tatatatcta acacaccgtg ataacaaatt
    17701 ttgtcaccac taccaaattc gtggtgcgtg tagtagtcat gcatacacca cggggaaaca
    17761 ccgacaatat ttttatcacc aaagaaacta tcaagcaccg ctaaatggcg ttttgtatga
    17821 ctttctaaaa taagcgggtt atcaaatggt ttagtggggt acatgtgccc attattttca
    17881 ctaacgatgt aaggtttttt actttttgtt actttacgct tagctaataa tcccttattc
    17941 gtgccgtcat gactaaagtc attataggag tagatgtctt caaaaagttc gctattagta
    18001 aagttacgtg ttcctgttgt agcgcgaacg gggtcaagtg cttttgctag tttatgacac
    18061 tcttcgtaga atgggtggtc agcgctttca ttaatcctaa tggaccacgc gataatactt
    18121 ggacgattaa agtcacgatt aatcatcgtt tcaatgtttt gtaaatgaat ctttcgccac
    18181 gcttcgtccc ctatatactg ccatcctgga acttcattta tgaccataag tcctaaaaaa
    18241 tcacaggcat caagaaaagc tctacttggt gggtagtgcg agcaacgcac ataattaaca
    18301 ccaaggtttt taagttttac aacatcataa aattgtagaa aactcgtggc tgctgcgcca
    18361 acatatgggt aagtttcatg acgattaagt ccaactaaag gcgtgtattc accgttaaga
    18421 aagaagccgt cactaagaaa tacggcactg cgaaaaccaa agacactaac atattgttta
    18481 acatctttat tagcggtaat agtagtaact aaattgtata aattaggatt attaacactc
    18541 caaagtttgg ggttttcgac tgtgaaatta tgctcgatac tactagcgat aagctttgtg
    18601 ccttcgtata cttcataatt tacagagtaa tcaaccttat taatgtttct aatatcgggg
    18661 tttacttcaa tttgccctaa atgattagcg gtaactttta catcaataat atctaatgcg
    18721 ttatatacaa aaagtgttac ttcacggtaa attccgctaa aggggaagta gtcaacggta
    18781 ttaccaaatg gtggagcgtt aactttttca ctgccgttaa cacatacaac aattaaatta
    18841 ttgccctcga caacatattt cgtaatatca atattttctt ccatatatgg tctagtaaat
    18901 gtaccgacca attgcgaatt tacatataat gtaaaagaat tatttacgcc accgaagcgt
    18961 aagacaaatt tatcactttc aagtaccttg gtaaaaacga tttcacggaa gtaagtacca
    19021 acactatcaa aatccttttc atcaaagtaa ttaaaaggaa agtcgaccat attatgcggg
    19081 aggtcaacac ttattacccc ttgcgggata gacttaatat attcatcatt aaattttggg
    19141 atgaatgacc aattatagtt cagtgcgtgt ttcatacata catattttaa cattaaatga
    19201 taattactta gtaaatatta aaaatagcgt atgtatgtta aaattaatct atggaataca
    19261 aatatataca ccttaaaaca aatgacacat catatgtgct tgccattaat aaatatggcc
    19321 atttagaaaa cctttactat ggcgcaaagt tagcgctaaa tgaagattta agcattttag
    19381 ttaataaaca aactaatatg caagggacga caactgttta tagcggagaa ccgcatgaca
    19441 cttattcctt agattttgtt aatttagaat tagggacgat tggcaaaggt gattaccggc
    19501 gaccaagtat tttacttgaa catgaactag gatttgcgag tgattttgtc gtaaaaaagc
    19561 acggttttgc cggaagagaa gtacttttag taaatgattt accaattccg catggctatg
    19621 atgaatactt ttatgtcatc cttgaagaca acattagtaa agtaaaagta caattagatt
    19681 acttaatttt taagcgcgct aatgtgatcg ctcggcgtct tgttgtcctt agcggaaaag
    19741 gtaaattaac aaaagcagca agtttaaact tggatttaaa taatgatcat tttgatgtgt
    19801 tttcaactta tggcacatgg gcaaatgaag tccatgcttc gcgtaataag ttagtgcctg
    19861 gtctctttga agtggcgaca agtgttggcc caagtagcaa tcgtcataat ccgtttattt
    19921 tacttttgaa cgagaaaact gatttacata aagggatagg ttatggtgtt aatttaattt
    19981 atagcggacc atttaaagct tcggtagaat taactttcga taatagggtc aggcttcagc
    20041 acggcataaa tgatgaaaca tttagtaaag aacttgatgg aaagcctttt attaccccgt
    20101 ttgccgccct tacatttagt gataaaggcg aaaatggcct tagtcaaaac ttccatcgct
    20161 ttgttataga ccatgtcgtg cgttatgaat atgcgcggaa accgcgtcca atcttactta
    20221 ataactggga agcaacttat cttacatttg atgaagcaaa aattaaaaaa ctaatgcgtg
    20281 aagcaagtaa attagggatt gaactaatgg ttttagatga cggatggttt atcaatcgtg
    20341 atgatgactt tggcggatta ggtaattacg aagtaaataa gaagaagtta ccacgcggct
    20401 taaaaggcct aagtgactac gcaacgaaat taggaatgtc ctttggttta tggtttgaac
    20461 cagaaatggt tagtgctgat cacccgatat ttaccgaaca ccctgaatgg gttgttaaaa
    20521 ttcctagtgt taaacctagt actggacgcc atcagtattc ccttaattta acgctcaaag
    20581 aagtccaaga ctatattgtc gaaaatgtga gtaaagtatt aaagagtgcc gatatcaaat
    20641 atgttaagtg ggattataat cgtaacttaa gtgattacta ttcaccatcg ggtgaatatg
    20701 gcacattctt ttatgattat acgcttggct tatatgcgat tatgcagcgg ttaaccaaag
    20761 aatttccaac gattttatgg gaaggttgtg caagtggcgg taatcgtttt gatttaggaa
    20821 ttttatcgtt ctttgatcaa atttgggtga gtgactgtag tgatgcttat gaacggatta
    20881 gaatgcatac caatctcgcg aaagcttatc cgcttagtgt catgtctggg catgttagtg
    20941 ctgcccctaa tcatcagtta ctacgtgatt taccactgca aacacgcttt aataatgcct
    21001 ttatgtctgg tggttttggc tatgaattag atgtgaataa tttaacgcca aaagacaaaa
    21061 aagaaattaa agcatataac gaaattgcta aagcattccg cagtgttatt atggacggga
    21121 agtattacga gttaacactt agtagcgcta aagataaaaa ggcgtacgca ctactaagtg
    21181 aagctagcga taatgcactc ctcttaatta gtagcggcat tcattcaccg atgccggaag
    21241 ttttaaagtt tccgtctatt cccgtgaagc aagacttaac ttacgaagta tttgccttgc
    21301 cgcaaagtac agatatctca acttttggtt cactgcttaa tatggtatta ccaaagttta
    21361 ttaagcatga cggggcagtt atcaagtacc ttgataaacg aagtagtgca gaagaattag
    21421 tgaaagtcga agtaatagaa cgctacactg taagcggaaa agcattgcgc gctggggcac
    21481 taacgcttta tcctgaatgg acggcaagtg ggttctcatc gacaacaaga atgacgcgtg
    21541 actttacgag cgtcttatac ttcattacca aaaaagatta atatttgcga taataggaag
    21601 gaaacgaggt atttactatg gtagaaattt tagtggaaaa actcttagcg tatgcggaac
    21661 ttaaactcgc tcttaatagc gaagatatca attattaccg caatgtactc ttaaataaat
    21721 ttggtctaaa aaaaccatat aaaggtgtca ttaatgttag tgaaattcaa agtatgaata
    21781 acccacaaat gttaattgat gaaatgcaac atttaattga aacaaaagga ttaccagcac
    21841 atttaagttt tacagaccct gaagaagtaa ttattgatgt gttagggatg atgacaccaa
    21901 tcccgagtgt tgttagtcaa cgctttgcgg aaattcataa gaaagatagt aataaagcaa
    21961 ttaagtattt atatgattta agtatcgcta ataactatat tcaagaagct aaagtggcgg
    22021 aaaatattcg ctggagagca gagttaagtg ataaatattt tgaaatgacg attaacttag
    22081 caaaaccaga aatagataat aaagaaacag taaaactatt aaaagaaaaa caagatgata
    22141 agtatccaag ttgtccgctt tgtttagaaa atgttggctt tggtggtaat gagtcaaccc
    22201 caccacggca aaatttaaga gcagtgccac ttactttaga taaagaagaa tggtacttac
    22261 aattttcacc atttgtgtat tataaagacc atatcatcgt tattagtaaa gatcataatc
    22321 cgatgacaat taccccacgt atcttttcaa aactcgtggc ttttgtcgat caattcccgg
    22381 atttatttat tggtagtaat agtgatattc cgattgttgg tggttcaatt cttaatcatg
    22441 aacacttcca aggtggcgcg aaagtgatgc caatgtttag tgcaaaagat cggtttgtct
    22501 taaaacataa acatgaccgt aaacttaaga ttagcattct tgaatgggat agtaatgtca
    22561 ttaaattaga atcaagaaca aagagccttc ttttagcaaa agccgcggaa attactggta
    22621 attggttaga atacacgaat gaagaattag ggattattcc cgcaactagc ggtgaacgcc
    22681 acgccgcggt cacaccaatt gttgttaaag aaaaacataa atacattatg tacttaattt
    22741 tacgtaataa tcgcactgat gagaagtatc caaccggtat attccacgct catcccgaat
    22801 accataacat taaaaaagaa gggattggcc taattgaaca aatgggaacc tttattttac
    22861 cagcgcgact aaagaatgaa tttggtttaa ttgaagaatt atataaaaaa ggtgttactg
    22921 gtgccgaaat gattagtgaa cacccaagta ttgctaaaca cgctactttt attaaagaat
    22981 taatgctcag taaaattaaa ccaactgaat ttgaaaagta tgcgcgtgat tatatgacta
    23041 atgtctgtga aaacatttta caaaataccg catgctttaa aaatgatgaa gctggacaag
    23101 acgcctttat taagttttta caactttaat aaagaattgc acgaattaaa gtgttaaaag
    23161 tggcgacaat gccacttttt tgttaacatt gagttatggg aacaaacagt aaatttaact
    23221 tagggattaa cttaactggt gatagcgatc ttgatcgtca taatattttt aattatatta
    23281 ttgaaaactt caccaaagat aaaactgaag cggagatttt agtattagcg gcgacttttt
    23341 taggtaaaga cacaaaaacg atttttaatg aaattcgtgt taaccctgaa gtacttgtcc
    23401 ctgtatgttt atatttaatg agtgaagaaa aaattgcgct atcacggcgt aatgaaattg
    23461 gtaatgcttt gcttaattac accgacgcac atgcgcggaa gattgatgat aaagcgacac
    23521 gcaaaccact ttataacttt acgcttgaat acgaaaaagc gttatacacg aaaatggcta
    23581 attcaaatgt tgaagatatt ttggtgacat tcccttttta ccgtatttat tatacgcaag
    23641 gcttgattta cgaagcaatg gccgattaca cgaaagcatt tgtgatgtat tcaaaagctc
    23701 atcaatggaa tccgtttaat cctaaaccaa ttcttaaaat ccttgaaggt ttaaagtatg
    23761 atggtcgcgc tcaagattta ttacggcttt caatgtggta cttgcaaatt acttatagcg
    23821 ccccaaacat tagtgcagca ttgcgttatg caggatatgc gctttatctt gatggtaagt
    23881 tcgccgaagc ttatgccttt tattaccaag caatcgtgta tcaagatggt gaaacaccag
    23941 cacgttttaa tgaagaaatt acaagtgtat taactgcctt gaaaaaagat gccccatacg
    24001 aattaagccg ggcacaaatt aaaaaacttt ttgcgtattc aaaatttaaa ccgtatccta
    24061 gtgaaatagc atttgatacg attagaccac taattattaa tcattataca aaaggtaatt
    24121 atgttgatgc tattcgttat gcgacacagt atgttatttt ccgtccaaat gatgataaga
    24181 taatgcgcat tttaaaagca agtaaactta atttagacta ataatattaa aaatattaca
    24241 aaaaatggca ctcggtgttg acaagtgcca attttagtat ataatagact tagcactcag
    24301 acatttaaag tgctaaggag gtgttatatt atgaatttta atccttttcc aaattatgaa
    24361 caagatgaaa aaatcttaga gaagtttgga cgctttttag atgaagacgc taaactaaat
    24421 aaaagtgacc cagtaattgg gcgtgatagt gaaattagac gcattattga aattttagcg
    24481 agaaagcaaa aaaataatgt tattttactt ggtgaaccag gggtcggtaa gaccgctatt
    24541 attgaaggac tagcgcaacg aatcgttgct ggtgatgtgc caaagaattt agcagataaa
    24601 agaatctatg aattagatat gggcgcacta gtggctggtg cgaagttccg cggtgaattt
    24661 gaagagcggc tcaaagccat tttaaagaaa attaaagaaa gtaatcatca aatcatttta
    24721 tttattgatg agattcattt aatcgtcggc gctggtaaag ccgaaggagc cattgacgct
    24781 ggtaatatgc ttaaaccaat gttagcgcgc ggtgaaattc atacgattgg ggcgacgaca
    24841 cttgacgaat atcgcactta cattgaaaaa gaccgagcgc ttgaaagaag attccaaccg
    24901 cttattgtcc aagaaccgac gcttgaagat acaatttcga tattacgcgg actaaaagcg
    24961 cgttacgaag cccatcacgg cgttaagata agcgataatg cacttgtgag tgcagcagta
    25021 cttagtgacc gttatattac cgaacgcttt ttacccgaca aggcgcttga cttagtggac
    25081 gaagcttgtg cattaattcg tgttgaagtt gaaagtttac ccgccgaact cgatgaaatt
    25141 cgccggaaga tgcggcaact tgaaattgaa cgagttgcac ttagtaaaga aaatgatgac
    25201 ttaagtaaag ttcgtttaca aaaagtaaat gaagaactgg aaaatcttaa aaaagaagat
    25261 gaagccttta gtaaagaatg gcaacatgaa ttagatgaac ttaatgtgct tggtaaggct
    25321 aaaaaagaat tagagcaagc acgctttaac ttaagtgtgg cctttaatag cggtaattac
    25381 gaagaagctt caacattaca atataaagta attcctgaac ttgaagcaaa gatcgaaact
    25441 ttaacaaaag aaactaaaga taataaagta ttaaaagaaa cagtaacgga aaatgaaatt
    25501 gctgcggtcg tctcacgcgc aacagggatt ccgttaaaac gaattatgga aagccaaaaa
    25561 gaaaagattt tatcgcttta tgatgaacta aaaaaacgcg tattagggca agatgtagcc
    25621 ctgcggcttg ttagtgatgc cattttacgg caacgcgcag gacttaataa cccaaatcgc
    25681 ccaattggta gtttcttatt cctaggccca acaggggttg gtaagaccga agtagccaaa
    25741 agcttagcct acgctctttt tgacgacgaa cgccatattg tccggattga tatgagtgaa
    25801 tatatggaaa aatttagtgt ctcgcgtctt attggtgccc caccgggata cgttggttat
    25861 gatgagggcg ggcaattaac cgaagcagtg cggcgtagtc cttattcaat tatattattt
    25921 gatgaaatcg aaaaggcgca tcctgaagtc tttaatttac tcttacaact actagatgat
    25981 ggccgcttaa ctgattctca aggaagggtt gtaaacttta aaaatactgt cattattatg
    26041 acgagtaact taggtagtga attcttcctt aaaaatcaaa catcaaagat tgatgactta
    26101 gtgaaagcga cttttaaacc cgaattctta aatcggattg atgaaattgt gtatttctta
    26161 ccgcttagta aagacacaca acgacaaatc acgcgcaaga tgcttgatga actaatggct
    26221 catgttgcca aaaataactt caagttaata tatgacgaaa agattgttga ctttgtgctt
    26281 gataatgctt acgctccaga atttggggcg cgaccattaa aacgctttat ccaaaagagt
    26341 ttagaaacat ttatcgctga acaagttgtt cagggcaata tcgatagcag tattacttat
    26401 cgcttaacgg taacaaataa cgaagttagt atttcgccct ttgcctaact ttaatttaca
    26461 aaaaaaggcc tctttgttat aattaaatca cataagaaaa gaggtctttt aattatgaaa
    26521 atacgtcgat tattattagc gctgatcgga gttacgcttt tagcgtcgtg tgaaccacct
    26581 acatcacaga gtattgaagt acctgagagt gaaattccaa gcgttagtga cattagtgat
    26641 acaccaagta tcgtcagtga aacatctaca actgaagaag ttacgagtgt tgaagaatca
    26701 agtgaagagg tgtcttctga atgggttgaa cctgaaccag tattaaaacc agctgaaaat
    26761 tggaatttta gaccactacc tgcttacact ggtaattttt ggaacacgat tgactttaca
    26821 ctccgcggtg aagagcttcg tcaagcactt gccgattaca tgtggtcgaa gttttatgtg
    26881 ccaggtgttt tagataatag agttacatac gatgatgctt tagaggcaat aaaagtaatg
    26941 gacgctgatc caaataaaac aggtaatgta ttatcactgt atgacttacg tagtcatccg
    27001 cacgctgggt accccggtaa atggaatcgt gagcacgttt tcccgcaatc caaactagca
    27061 gatggtgata aaagtttacg cgctagtggt aatactaaaa atatttcaag tgacatcgct
    27121 aatttattcg cctgtgatgc tgctttaaat gaacataaga gcaatcactc ttatattgaa
    27181 tggaattacg ccgaagatcc agagcgttgg ttcccatact tatcacgcaa taccgctgga
    27241 cagctcgtgg acgcgattgt gcactacggc tactcaccaa cgccaattgt gcgtggtgaa
    27301 attgctagag cgcaattata tatggtacta atgtacccag atcgttgtag cattagtgaa
    27361 aactttcaaa ttgccgatat gattcgctgg gaccaagaat gggctccgac tattgaacgc
    27421 gacggtcaac ggcaaaaagg gattgaacaa tatcaaaaga ttcgtaaccc atttatcgat
    27481 aatcgtaatt tatcgtgcta tatttggggt aacactaatc gcgctacccg caaactttgt
    27541 gcggatattt attagaaata attaatgcac taattaaacg gtgagtcatt ctcgccgttt
    27601 tctttatgca aaaagaggct attcattcat taaatgtatt acatttattg taatatttag
    27661 cgttatgtac tataattcaa ttcgacgccc ccttagttaa atggcataac agatccatgg
    27721 taaggatcag tcgctagttc gattctggca gggggcacca ttgaaaaatg aattagcttc
    27781 caaatcttgg agagaggtat aatgaaggta cctacccaag aagggagcta ttttttatgc
    27841 ggataacgat gaaagagaag gtcaagatat gtaaaatgca tatcgaagaa ggtctttcga
    27901 tttctcatat ttccgagctt tatgggggat atgataaaac gaacatcaag tacataataa
    27961 atttgtataa gatgcatggt gaggagccat ttttgagccg tgtgcggcga atgtataagc
    28021 gtgatacaaa gttattagca attggacagg tgttagcggg aagaacgata agagacgttg
    28081 cgtgtgagca cggtttagcg aacccgggag tattaagtga ttggttgaag aaataccgtg
    28141 aaggcggcga agaagcgatt caagatacga aagcgcgagc gaattactta ttaaaggaag
    28201 accgttttaa agcggttgtt gatcgaaaga ttgaagcaga gaacgagcgg ttgcgtgcgg
    28261 agatagcgta cttaaaaaaa tcgcagtcct tagctcaaaa actagaagga gcgacggttc
    28321 aagaaagagt aagaatcgtc gatgagttaa ggactaaata caaattaagt atccttctag
    28381 agctgtctgg tttatcgtca agcgtctttt attacacatt aaacgcgaat aaaacaaggc
    28441 aaaataagta tgttgagtta gagaaagaaa tagagcgatt attcattaaa gttcataaga
    28501 aacggtgcgg atatcatcaa atatacaata atttgaagaa aaacgggtgg attgtcggta
    28561 aaaacaaagt tttagaaatc atgcgagcga aaggttttag taaacccaaa gtcattaagt
    28621 ggcgaaagta taattcgtac gaaggaaacc ttggtttaaa aattgaaaac attatgaatc
    28681 aagattttaa aacaacaagg ccatatgaaa aaggcgggac ggatgtgaca gagtttccaa
    28741 tgtttggtga atcagtatat ttgtcacctg tgattgattt tcatacccgt gaagttctag
    28801 cgtattctgt gggaactgat gcgaaggtat atcgaatcat ccgaatgtta agagaactcc
    28861 aaaagaagca cgggaataat cttatgggga tgatacttca atctgatcaa ggcgttcaat
    28921 atcaaaactc aaggtatatt gttgaacttc gaaacatggg gataatacaa tcaatgagtc
    28981 gaaaaggaaa ttgccttgac aactctccaa cagaaaactt cttcgggcgg cttaaacaag
    29041 aaatttggga caataacaag cgtgattatg atagtgtaga agctttaata acaacgattc
    29101 atgaatatat aaattactac aataagacaa gaataataat gaaaacaaaa cttacgccta
    29161 tagagtgtag aaataattat ttgttaagta tataataatc tcgtaggttc attaagaatc
    29221 tctccaactt tataaagcca catcacgaaa aaggccgatt atatgggcct tttttctttg
    29281 cctggcgtag atttggcgta gatttggcgt agatgaacaa taagtgtcaa ttattttcgc
    29341 catctataaa cgtatatttg tcttaaatta cagcaattat aaaaagataa aggttctaaa
    29401 attccgaact ttagtatgtt ttatgagtta tatagcattg tgtcaaaatc cccaaaaaga
    29461 acaaatgaaa ttgttcgaat tatgaaagag aaatcagctg ctgatttaaa tgaaattata
    29521 attttttaat aaaacgttgt gaagtatgtc accaaaagtg taacaaacgt agcaaataaa
    29581 cgaaaataac aaagaaagcc aacttaaatg attaagaatt ggtgttttaa taaaataaac
    29641 ggcaaaaaaa ttgattacgt gtattaatcg cactataaaa actttgtttt cgtgtaaaat
    29701 aaggattgca aaaactttgt tttcgtgtaa aatggtgtta gagaggtaaa aacaatgatt
    29761 tttaaaagaa aaatatatag tgacttactt aactggaaaa gtgaaggtgg caaaacagca
    29821 cttttaattg aaggggctag gcgcgttggc aaaagcacga ttgttgaaga gtttgcaaaa
    29881 caacaatata aaagttatgt actaatcgac tttagcattg ctagcgatga tgttaaatct
    29941 ttattcacta actatgctag tagtttaaat gattttttct ttttcttatc ttcgttaact
    30001 ggtcaaaaac ttttcgaaag agaaacgtta attatatttg acgaagttca actttttccg
    30061 gctgcccgtc aactaatcaa acatttagta aaagataacc gctatgatta tattcaaact
    30121 ggatctttat tatcactcaa acagaatgtt gcggagatat tactaccttc tgaagaacgt
    30181 acaattaaaa tgtatccact tgattttgaa gaattttgtt gggcaacggg gcataatgat
    30241 cttattcctt ttttacaaaa acgatttgaa gagcggaaac cggccaaaga actacatcaa
    30301 cttgcgatga aattgtttcg ccaatatatg cttatcggtg gtatgccgca agcagtagca
    30361 atgtttttag aaacgaatga ttatactcgt gttgatcggg caaaaagaga tatcttgcaa
    30421 ctttatcgga atgatattag gaaatatgcg aaaaacaatg aatataaagt tattagtatt
    30481 tttgatgaaa tccccaatca gttaacaaaa catgaaaaaa aattcacact cagtgctctt
    30541 agtaaagatg cccgttttcg tgattatgaa gaagcctttg tttggcttaa cgaagcgatg
    30601 atgacaaatc cttgctttaa cgcaagtgat ccgaccgtag gacttgggct ttatcgtgaa
    30661 tccttaacac tcaagatgta tatgggcgat accggcttgc ttttcagcca tgcttttaat
    30721 gaaaatgaga ttatgcaaac agaagtagcc caaagtattg ttgcaggaaa attaaatatc
    30781 aattctggga tgtttttgga aaacatcgtt gcccaaatgc tcgtggccag taataaacgg
    30841 ctttattttt attcgcgcaa tgatcggaag aatgctaaaa atacaatgga aattgatttt
    30901 atgattagtg atccacataa cccacggaaa atatcacttg ttgaagtaaa atcaggtaaa
    30961 aattatacaa cctcgtcttt aaagaaaatg aaagtaaaat tcggtaataa attaggccaa
    31021 tcgtatttgt tacatactaa agacttacga gtccacgaag gcgttattta tttaccagta
    31081 tatatgacaa tatttcttta aaacgaaggt tcgttaacat aacgagagta gtattttaca
    31141 aaatcttagt tattttaagt ttcgcatcga taaaacttta aaaatcaatt tttttctagc
    31201 tataaatcac acttaaaaat aatatttatt ggaaaaagtg taaacttaag ataaggattt
    31261 ttttaagagt ttttaacaaa aaaagttttt gtcacaaaac acgctttgta atcgttatgt
    31321 attatagtag agaaataatt agtactaata gagaggagca aattgtactt aatgtcacaa
    31381 aatgacgtta agttacgttt gtattatgta agaagaaaat gactaacgaa cattttgcga
    31441 tcgtttatga aacttacaaa gtgatggttt tccaaattgc ttactcttac ttacgaaatg
    31501 tagaagatgc ggaagacgca acgcttgagt catttatgcg cctcttacga ataaaccgaa
    31561 aatttgcatc aggtgacgac gaacgacgct acttaacaag aatcgttgtt aacgtaagta
    31621 aagacatgtt acgcaagaaa agaccagtgc caattagtga tcccgtgatt gcagttaatg
    31681 acgcagggca aaatgaaaac aattatattt gggacgaagt taacaaactt cctaataaat
    31741 ataaagatgt gattatctta cgttacatca atgaacatag tttcaatgaa attgcgagtg
    31801 tgctaaaaat aagtgcaagt gccgcaagaa agcgtcatga acgagcgctt aaaatgttga
    31861 aggaggagct tagtcatgaa taaccatgaa gagaaactaa aaaagcttaa aaatgatatc
    31921 cccgagtatc ccgatggttt agaccaaaaa gttttcaggg ccgctgaaca agcaccacgc
    31981 tacaagcgac ccggctttcc taaactactt attccctcct tgctaaccgt aacagcagtc
    32041 gtgttatcaa ctttctttgt cacaaagtat gtttatggct ggaacacgcc ttcaaatacc
    32101 ccaagtaatt caggaatgcc aagtgaacca tctaatacca gtgcagaaga aggggaagtt
    32161 tatgattatt atgtttcggc aagcgcagca ataccgggtg cgtcccttgc cccagaaatg
    32221 cttgttgaag ggaaactagg ctattttgat atcgtttata ttgattcaca gcttgcgcta
    32281 gatgcaacaa ttaatcaata tgctgatgac gcacacccag tacttagtac attaacgagt
    32341 gtaattagaa ataacgcgga attctttagt gaaagcgtct tgataattgt cccgtttgcc
    32401 catacaagta cagaagaagg tattgaactt acacaaatcg aatttgggag taaggagcaa
    32461 ccaacaaaac tgcgtttcga tatatattca ccggaattta atgatatgga tacccttgtg
    32521 acgtttcatc ttgtcacgat aggtgaatgg gcaagtgatc tattaagtga aagtgcgttt
    32581 aaaataagtg tacataatac gtttgacaat agtaataaat cagtttttta tagtaattaa
    32641 tggagggaaa atgaaacgaa cgcaaatttt agtcgccggc ttactcttga tattaacatc
    32701 atgcagagcg gctaatcacc aaacacttag tgaagttttc ccaatgctta aaacagagga
    32761 atttgctgaa gttaaacata ccattagttg taatagtctt gcacctttag cgcttattaa
    32821 tcattactat aacgatgacg tagccgccgt aacgaacttt gttaatgaac tagggaaaac
    32881 taaagtgcgt cgttacgcaa aacttcctga aagtaaaaat gttgcgtgcc cgacaattaa
    32941 tacgtacgct tttaagaatg aagctctcga gtttacgttc gatgtcacaa atatgttcgt
    33001 tgtgaaagat gatgtgtatt atgaactaga agaattacca aatattgtca ctacatacag
    33061 ctcgtatagt tttagcggtt ccacaacgat tacggaagtt ttcaatagcg agggaaaaga
    33121 aataacggat ttttcgcttt ccttgccttc actagaattt aaagaactaa cggagcttag
    33181 tggaaataat gtaaacttca cgattgaaac agtaggcggg attatttctg tgtatgacgc
    33241 agttactttt ggccttaatg ttggggaaca aatagagcgg ttttatgagg ttataagtgg
    33301 tgacacattc gcaaacttat ttgacactaa caataatttt acagatgaca caatgctttt
    33361 agcgaacatc ccccaacaac ttacttttag aaatcaaaaa tacaaaatga tcaccagtgg
    33421 ttttttcgaa tggcaagaca ttcaactaga tttgctaatt gcttatttcg ttaatgaacg
    33481 tgactatgat gtgttttatt tagaaaatcc cgatatcgaa tattgtattg atgaggcgaa
    33541 tacactttat tatgctgctg gcccaagtaa atttccgctt tattcgctcg ttggtcatgc
    33601 tatcgagcaa ttacttggct tattgctccc gcaaggcgga ggttcgccaa tgatatttct
    33661 taatataagt tgcgtctagt atcgcttggt agtgtatgaa gtagaaagcg tttaagtaaa
    33721 aacaaggtga catgttgaat gaaaaagttg ttaacgagtt tcttattagg attattatta
    33781 ctagctggtt gcgaaaatac tcgcaagtat cgttttacaa ttattgacac ggatcgaaca
    33841 gttttatcaa aacataaaga tcgttattcc gctggagaag caatacggat cgagacagtt
    33901 atgttaactg acgttattgt tagcatgtat gtaaatgacg aattttataa taatgcaaca
    33961 accgcggaaa ataatgttgg tcaaagtgtc tttgtttatg actttatcat gccaacttat
    34021 gacgtaacga taacgttcgt cataaaagat gggatgattg tggaaccgaa ctatattgat
    34081 ataacgttta ttcagcgcaa gattgttgat tggcagaact ttgaagatta tttaattccc
    34141 gtatttggcg aagaagactt aacaataaca actttaagtt ttgaagataa tataaaacta
    34201 acgcttgaag aaattgatga tttacaaagt cgaagtgttt taaactatat cccacccgaa
    34261 acaatgtatg gatattacat attctacggt ttttattttg atgaagaaaa cactaatgat
    34321 ttacataggt taacaccaga gtttgcagta actctttaca acacgtacta ttacggacta
    34381 gcgggataaa ttgttctaat tttgcgtact ccactatttt ttgtttattg gaataacctt
    34441 aatatattta tataaaagaa aggcgtgttt ttggcgtaga taaaaatcta aaaagtaaaa
    34501 tcccgtaaga tgtggcaaga tgtggtaaga aaacaggttt taaaaaacgc gtttttaagc
    34561 caaaataaga tgtgataaga cgtggtaaga tgtggtaaaa tgaacggcga taaactggca
    34621 gggggcacca tatcacgaaa aaggccgatt atatgggcct tttttcttta cctggcgtag
    34681 atttggcgta gatttggcgt agatgaacaa taagtgtgta taggtaaaaa aataacacca
    34741 ttttcaaact aacatgaatt acataaactc tt
//
LOCUS       DAOSTA010000008        30842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_1626_length_30842_cov_9.059051, whole genome shotgun sequence.
ACCESSION   DAOSTA010000008 DAOSTA010000000
VERSION     DAOSTA010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 30842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30842
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1626_length_30842_cov_9.059051"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PLU08_01545"
     CDS             complement(<1..576)
                     /locus_tag="PLU08_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70426.1"
                     /translation="MKLKKLLPLSFIFLVITSCDSGIKVSKQKANKAVFAAKEKSQFH
                     NFDSVNFTYIGEVLRGKVVYNLDKTTESEVSHFSLDVKAIATDIGSDDLKMAVNYIHY
                     EPSEDDENVFEPLYKRDNYYTEEWFYTKKESIPPPTNAGVSDKLNKKLKCKMGKLPAT
                     DVLYFFNNYYGSLLIERVPRGIAEYPGFLSIV"
     gene            complement(673..1758)
                     /locus_tag="PLU08_01550"
     CDS             complement(673..1758)
                     /locus_tag="PLU08_01550"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOQ70427.1"
                     /translation="MDKKRLELLRKVSEVKGISGAERDVARLIKAELKDVADSFEYDN
                     LGSVIANLNGEEDAPRVLIAAHMDEVGFLVKQIEKGGFLRLHNVGGWWGHVIPAHEWV
                     VTTRDGKEYIGVTGAQPPHGMPAEQRNKVMELKDMYLDMGVGSDEDIKKLGIKVGDTV
                     TPLQTFRVLNDGKTLLGKAWDDRVAVAIGIEVLRRLKKEGHTANVSFAGTVQEEVGLR
                     GARTSAYKVKPDLAFALDVTMSYDLPGSPAMDTKLGAGVALSIMDGSVIGHRGLFDHV
                     ESVAKEIGVKYTYDMLTAGGTDSGEIHKVHDGVINMTLSLPCRYFHSHVSLVHSDDID
                     AAIDLLVAVIKGVDSKLIERLKATKYE"
     gene            complement(1780..2280)
                     /locus_tag="PLU08_01555"
     CDS             complement(1780..2280)
                     /locus_tag="PLU08_01555"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF020341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOQ70428.1"
                     /translation="MEINLAPLLLKQRELDADINSRHNVTHESTKTERFLALLVEIGE
                     LANATRAFKYWSLKKSESRDVLLDEFADGLHFFLSIAIVFNFAVGKVEYESLPATKEE
                     VNNAFKRVYLRITYLMRKESKRCYTVALKEFLSLGSALEFSAQDITNAYNAKLVVNYD
                     RQQHNY"
     gene            complement(2270..4684)
                     /locus_tag="PLU08_01560"
     CDS             complement(2270..4684)
                     /locus_tag="PLU08_01560"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOQ70429.1"
                     /translation="MFTTTKSFIHEFKKRLTEKYGVGLDDSHITERYDILGSMVRDYA
                     NINWRKSRDAVNKKGKKQLIYFSMEFLIGRLLTNNLINLGIYNVVNKGLKQIGMNLHD
                     LEEQESDAGLGNGGLGRLAACFLDSIASLGYAGNGHCIRYEYGFFRQKIVDGKQVELP
                     DQWLVNGNVWEVRKPKHSVNVKFYGHAETYQKRNGEFGIRTVNPTIVKAVPYDMSVVG
                     YRNDVANTLRLWSAEPSDEQLPRDESFDDYLRTLREINRGLYPDDSTEKGKMLRLRQQ
                     YFLVSAGLQSAIRGHFRRHKTLRNFHEHYVFQLNDTHPILAIPELMRILLDDYEYGWD
                     ESWDIATKTFAYTNHTIMVEALERWPVRYVHHLVPRNYMIIEEIHRRSQREFVNKGIS
                     EGDRQNMAIIRDGMIHMTNLAIYASFSVNGVAALHTEILKAQTFKEFYSLYPEKFNNK
                     TNGITHRRWFVASNPELNKYLTSLIGDSYITNPDDFEKLLPFVKDKKVQRKFLQIKRL
                     KKQQLAAYIKRANGIKIDVDSIFDVQIKRLHAYKRQLMNVFRIMMLYFRMKSDADFRI
                     HPVTFIFGAKAAPSYYFAKKVIELINGVADVVNNDPKISPFMKVVFIENYGVKTAEII
                     IPATDISEQISTAGKEASGTGNMKFMMNGALTLGTLDGANVEIHERVGDEHSFIFGLT
                     EPEVKALRESGTYNPWDVYHNDHEIRRILDSLIDGTWSQGNPHTFKAIFDEVMNKGDE
                     YLILKDLPAYFKRTDEIVTYYRKKSSWAESALYNIAKSGFFSSDRTIHQYIDEIWHID
                     KVKNGN"
     gene            complement(4721..6778)
                     /gene="glgB"
                     /locus_tag="PLU08_01565"
     CDS             complement(4721..6778)
                     /gene="glgB"
                     /locus_tag="PLU08_01565"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOQ70430.1"
                     /translation="MSFIDEFLMGFTTSAHKYFGAHFNPAKGKKKKSVTFRLYAPAAS
                     DVSVIGDFNGWDPNVHKMEKVDYRGIWEITIPELENYQNYKFRFRNAHGHYVDKADPY
                     AFFSEKAPGTASRLFDYEGFIWHDEDWMKSRCRGHDKPISIYEIHLGSWKGKENGRIL
                     SYEEIADYLIPYMRDNGFTHVEIMPITQYPFDGSWGYQATGFFSVDSRYGNPMQLMSF
                     VDRMHQAGFGVILDFVAVHFAIDHYGLIEFDGTRMYEYQDSEHTFSQWGSPQFDLGRD
                     PVRSFLISAIFYFLEYFHFDGIRIDAVSNIVYWHGNSDRGVNHGGVEFLKRLNGKVHY
                     HFPGVMMIAEDSTAFDGTTRDVEWGGLGFDYKWDMGWMNDTLRYYSKDPIYKKYDHNL
                     FTFSMHYFFSENFMLPLSHDEVVHGKGTIINKMFGNYDQKFALTRNLYTYQFAHPGKK
                     LTFMGNEIASFDEWNESKSLPWNLKSYPKHDSVSRLVRDLNLIYRSEKAMHVGEFDPR
                     NFRWLMVDNRDQSVFAFQRKYGEETLIFVFNMTGNYYEYYDIGVEEEGTYTEIFNSDK
                     DVYGGYNQYNGLPVTTHAGPGPEMRPYRLTLKLGSFAAMILKKDLPAKKEEPKVEEVK
                     AKKPAAKKAPAKKAATTKKAEPKATKTATKSTATKKATKSATAKKTTTKKATTAKTKK
                     DTK"
     gene            7146..8354
                     /locus_tag="PLU08_01570"
     CDS             7146..8354
                     /locus_tag="PLU08_01570"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOQ70431.1"
                     /translation="MSKLVLAINSGSSSLKFKLFKVPSEKVITSGLFERITQKMGAFR
                     IKTETGEVRRELPLPNHEVAVNVLTKALIEFKIVKSLDEINAVGHRVVQGGKYYNDSV
                     EFTDEVAAMVEKLSPFAPLHNPANLTGYEAFKKILPDALHVAVFDTSYHQTMPPEDYL
                     FPIPYEYYEKYDIRRYGAHGTSHKFIADEVTKINKRKPCNLIVCHLGNGASITAVKDG
                     KCVATSMGFTPLGGIMMGTRTGDLDPSVFYFVSKLEDKSPEEVYDMFNKKSGMLGVSG
                     ISSDARDVENALLQGNERAKHTFDLYSRRVADYIGQYHIRLGGTDTIVFTAGIGENVP
                     GIRKQIIDKVKDAMGIEFDDEQNSLTRGGIYGLLSTKNSKVKVYVIPTDEELMIVRDC
                     VRIAENKKKK"
     gene            8364..9353
                     /locus_tag="PLU08_01575"
     CDS             8364..9353
                     /locus_tag="PLU08_01575"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOQ70432.1"
                     /translation="MHIYSKEIELKIKEYKKTTKAIFSLVEKYDKVAIFTHLSPDFDA
                     FGSQFGLGVYLRDHYPEKDIKIIGQSLPLYNGELYPEMDEVKDEWFNDDFLAIIVDTA
                     TLDRVSDNRYEKANHLIKIDHHPNVDPYGDICFVDVTSVAVAEMIAVMLLADGGTISK
                     DVASYLFSGIVGDSGRFKYASTTSLTFGIAEILTSLGIDLNYIYNRLYIQNLDDLRCI
                     GYILNNFKITKNGFAYYVMDEATLQELNITAERAKENVNVFADIRGIKVWAAITERTD
                     TNEWRVSIRSAGIPINEFAQKWRGGGHVQASGASLLNREEIDVFVKEMDEYIG"
     gene            9401..12388
                     /locus_tag="PLU08_01580"
     CDS             9401..12388
                     /locus_tag="PLU08_01580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOQ70433.1"
                     /translation="MSFIPLNVFTNYALLQSALTLDDYLKTLKNRNIKAAGVSDPDTL
                     FSYPHFAKKSREFHIKPLYGVRLNFEGSSLVIYPQNEKGYRELLALLEVKNKRELTLQ
                     DLKENSTNLIVIIPSSAIHNLELKEQIMQKYFYNFAINTTSLYIGLERENAELTSFLR
                     EFSDKYNYALVAFPLIKYAKSEDHKTLLMIEAIREGKSFSIYDISSGPDYILSDAELN
                     NYYSENELKNTHLISDLINFDLSVKHDLNFPLPGAYDPNKKIDELTLNALKAKGLDNN
                     KKYIERLTHELSIIRNLGYVNYFLIVSDYVNFAKKAGILVGYGRGSAPGSLVSYLLGI
                     THADPLEHNLLFERFLNPERVSLPDIDIDFMDIRRGEVIDYIKKTYGEDKVANIVTFQ
                     TNATKASLRDTGNVFNIDPKFINRLSNSLGNTNYNFRDAYRNIKAFRDLVDNDTYNLE
                     IIKNATKIEGFPRQSGLHAAGIIIDGEPLARNLPTFFNNGLRVTQYEMGFLEEHGFLK
                     VDILGLSNLTFVHNIINLVKENHNIDIDFYNLKFDDPQIYDVIRNGHTLGLFQLESAG
                     MRKAIATIKPTNFDDVSALLALYRPGPMKYIDEYALRKEGKSPITYINDDLIDILKPT
                     YGIMIYQEQIMQIAATMAGFSLGQADILRRAIGRKDEKIILEQKDAFFAGAKKRGYSR
                     KDTEKVFDDILRFADYGFNKSHSVVYAMLTTALAYLKTYYPYEFYQEILRGLVSDNVK
                     FSLARQELKALNIKISRPCVLKSTTEFIRTNNSINFPLSAINGLNRESANIIIRLREQ
                     KAFSSVQDFFFRSFKAGVTAEEFIALIEAGALDSFNTNRAQLKVMFNTYLPTLDIGLF
                     DNEAALHAIIVPKIKESDDRLDLEISRLRFPLSGNPLDKLKLKDIKRVSHILELEDGY
                     IKTYGVLTSLRLIKTKKGDEMAFATASDYEEDINLVFFPRVLAEVKGMLTRNNIYIIA
                     GKLETRDGQKQLVVNTLERVQNE"
     gene            12381..14936
                     /gene="polA"
                     /locus_tag="PLU08_01585"
     CDS             12381..14936
                     /gene="polA"
                     /locus_tag="PLU08_01585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOQ70434.1"
                     /translation="MSKRLIIIDGNSLLFRAYYATAYMGTDKIMRTSTGVPTNAIFAF
                     ANMIVRIVNDLKADDHLFVAFDTGEKTFRHEEYDDYKANRQAAPDELVTQFPIAREFL
                     DALSIRHDEVVGYEADDLAGSLAKAAGKEGFKVELYTSDQDYLQLIDEDISVQLIRRG
                     LSDVRFLDPKGLYEEFGYHAYQVPDFKGLTGDASDNLKGVPGVGKVTATKLLAEYDTL
                     EKILENADNIKGKLGENIREHKATALQSKHLATILTDLPLPCPLNDFHYKGFDNSAVH
                     AFAQKYEMRQFLNNLKYTNSEVEEEIKAHEIKSFNEVKEKDYSYISFYVTASDEDYYK
                     ATPEFIYFSLGKNVYRISFNDLLLDPKLKALFSDEALLKYTYDYKRHAIMLSNHALDI
                     KGTVNDALVLTYVWDTNVAPNISAFLLTYGVNASSEEEETYAILVAKLSAIYQEGINK
                     LKKENLLQVYEDIEKPLTDVLIKMEKAGFNVDKDVLTAIGSEYKVILDNLTRTIFLLA
                     GHEFNINSPKQLATILFDELGLKANRKRSTSADVLNDLKDKHPIIPAILEYRKYQKII
                     STYIDGFIPHIHRDGKVHTTFNQALTTTGRLSSNNPNLQNISIRHEEGREIRKAFNAG
                     DKDVLAIDYSQIELRLLAAIANVPSLIDIFNTDRDIHSETAIALFPLEDLNYARRKAK
                     AVNFGIVYGISDFGLSEQLETTVKDARAIIQSFFKTYPEVWTYRQEVINELIKDGYVK
                     TLTGRKRYIPQINDPNYQTREFAKRAAMNAPLQGSAADLIKMAMIKVDEFLTKNKCKT
                     KLILQVHDELIFALDQDEHEIVDDLVNIMENVLDLPVKLKVEVKTGKTWFEAS"
     gene            14942..16315
                     /gene="mutM"
                     /locus_tag="PLU08_01590"
     CDS             14942..16315
                     /gene="mutM"
                     /locus_tag="PLU08_01590"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HOQ70435.1"
                     /translation="MPELPEVETVKKILNKVIVGAKITDVIIHKENIITGDPHTFKKT
                     LIGETFSEVKRIGKYLIFTFESDLIMLSHLRMEGKYIEVLPGTEDSRYARVIFVLNDG
                     RRLCYDDSRQFGTLELSTKDTYLQTKSMQNVGREPFDIDPIDYFNKISNRTTPIKQTL
                     LDQKLMSGLGNIYVDEVLYLSKIHPETPTNMLGLMAINLLIENSIKVLNAAIKKGGTT
                     VRSYSPAEGVHGNFQQELNAYGKAGERCPVCNFKFKKIFVGGRGTTYCPSCQKNPAVP
                     LTVAITGQKASGKTLIANYLRKNGHKVIDTDKLAKELYKDIKITTDLEKHLGVNLHDN
                     TTFNLQYLRNYLVENENEIKKLNDFIHPLVKKEIIEIIKTSNEAILYFEVPLLFSHKI
                     YELFNFMIGVEVSVEKQLKNLEKRGDTHTINADEAYMKNRHKLDLIIVNDGSEQDLIE
                     KFKAFKV"
     gene            complement(16305..17183)
                     /locus_tag="PLU08_01595"
     CDS             complement(16305..17183)
                     /locus_tag="PLU08_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70436.1"
                     /translation="MKKVELDIDTTNVEEIRQEMYDKIMSHKSFVKLLEEKGATPEII
                     KNNVGLLNDYLESYLSVEKCRKAGHCVSDEKHHALTIQIDAPFVTVERHLCPVYLESQ
                     SLAMRFIVRDFPENYLDLRVEDLPARRGFAAFIRELMKVLKGDIYFIYAYGTAGIGKL
                     KTTIPFVTKMLEDDKNKTAGVVNFPAFVRASIADYYDKKDIVDSYLQHYIDLDVLVLD
                     NFGNEEVNKIVIESIIFPLLAARVRQKKPTIILAGLSTNDLRSLYVGRSVRGHQIVDL
                     IKANIKEEIYLQGTKL"
     gene            complement(17180..18427)
                     /locus_tag="PLU08_01600"
     CDS             complement(17180..18427)
                     /locus_tag="PLU08_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70437.1"
                     /translation="MRLIKSNDFYSVKVRGYVTSLHLEVLDILYQPIMGFAATALYRY
                     LFTMRDFRKGDPLSFTLIHDHFGFSYDQISIAFSKLEGLGLMRSFYVEYNEFSEYVLE
                     LYAPKDPAAFSQDQIFMNLLNDVLGPRHTQDLLSLFTLDRETLNKEEVTSTFAEIYAD
                     RIFDIKGDDLYKGEPLENVVGNIKFEFDRAAFVNILTRERKILPSALSTEELKKAEQM
                     AGLYNLTEVATAERVGDFYDALQPFGARVDFVAMNKHLQELVKYPVLSKPLRRPVQEL
                     AGESQKIKRINLMETYSPVEFLQYLNEGTKIAPADYNILTTLSHDYNLLSPVINALVY
                     YTLYHNNNELIRALVEKNASLLARNKVTTALDALDTLERPIKKRGRAPKKAPILNDDK
                     KEEATPQVDDLDDPIWDELKKLK"
     gene            18628..19590
                     /locus_tag="PLU08_01605"
     CDS             18628..19590
                     /locus_tag="PLU08_01605"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOQ70438.1"
                     /translation="MTVKVAVLTSGGDAPGMNAAIRAVIRSGLLAGYEMFAIFEGFRG
                     IIENKIVSVDRTFVSEIIMRGGTILKTARFPEFRDSKVVEKAAENLRFYGITHLIVIG
                     GDGSYRGAYALSKHGIKIIGIPATIDNDVMGTEQTIGFDTALNTITEAVDKLRDTSSS
                     HHRCSIIEVMGNRCGDLALYSGTACGVDLIISPEYRPSEEEIIETLKNINLNRARHAL
                     VIVSEKQFEDLEKLRERIVEETGFDAKIEVLGRIQRGGSPTASDRVLAGRLGVYAIDL
                     IAKGIDNVCVGLVDGRLRHHAIAEVTKSTNPNLDELLNVIDKLK"
     gene            19605..21050
                     /gene="pyk"
                     /locus_tag="PLU08_01610"
     CDS             19605..21050
                     /gene="pyk"
                     /locus_tag="PLU08_01610"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOQ70439.1"
                     /translation="MLNISKKTKIVATIGPVTNTLEKVRELIDAGMNVMRINFSHGTY
                     ETHKTIIDISKKLIEEHIYIPIMLDTAGPEIRCHFFEDGKVEIVKDSIVTINMKEVLG
                     TATEFSVNYPKLVDDILVGDMIKIDDGKLRLDVIEVDREKRLIKVKAFNTHVISNRKG
                     INIPGERMTLPALSERDKKDIKFGIENGIDMIAASFTRCAADVIQLKNYLAELGAPEI
                     PIIAKIENKQGVDNIKEILEVADGIMVARGDMGVDIPPEVVPVMQSDIILECRKVGKP
                     VIVATQMLDSMQTNPLPTRAEVSDVATAIKEGADAVMLSAETASGLYPVESAAMQQRI
                     AITMEEFLPYTQLANFGYNYSKKKRSDAIATSVASTANLIDAKAIVCFSETGKSAICI
                     SKSRPKCPIIMVTSSPAAGMRVAPYFGIYSIVVKTLPQLIEDMEAFALIKMRQLGLKP
                     NDNIIITGGTPTGTGSTNFLRIVALNKLGDI"
     gene            21050..21892
                     /locus_tag="PLU08_01615"
     CDS             21050..21892
                     /locus_tag="PLU08_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOQ70440.1"
                     /translation="MFIIALDIGGTNIRAAIADQNGKLLEVVKEKTLRHDKEIFITQV
                     KGIITQLDLSKYDPIAISIGVPGRVRQNGFIDELPNIGITNIDLVSALQKTFKLPVSV
                     RNDAEMAAFAEAALGAGKELKSTYFVTISTGIGGCLIVDRTIKNPSPEIGHTLVPYRG
                     NFYELEKIASGDGVINLAALNEIYYANAQAFFKDVTYKVPYALKIFDEWLDLITDFFI
                     FVNEAFTPDVIAVTGGVLKSKKLFWDKFIERVPNANIQIAHFSEDAGLVGAASYGFKV
                     FASQ"
     gene            22156..24054
                     /gene="thrS"
                     /locus_tag="PLU08_01620"
     CDS             22156..24054
                     /gene="thrS"
                     /locus_tag="PLU08_01620"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOQ70441.1"
                     /translation="MKELKYNNQLVKVNDGTTGFDLLKILNIEGRSNVLVYKLNGELF
                     DLATPVEQDGEFSFVTKEDEEAFAILNHDAAHLMAQAILSIYPGAKFGIGPDISEGFY
                     YDVDFGDVVINDGDLAVIEAKMMELSRADHRIERQVVSKEKALEIFAKNEYKVELINE
                     IEERITIYSQGDFVDLCYGPHMPSTRYVQHFKLLSLAGAYWRGDASRKQLTRIYGVAF
                     FSKDALDKHLYNLEEAKKRDHRKLGRELGLFMLSEYGPGFPFWLQKGMTLYHELEHWW
                     IKKQREAGYEFVKTPQILSRELWETSGHWLNYRENMYTTLIDERDFAIKPMNCPGSIL
                     IYNNGLHSYKDLPIRMAEHGHVHRHEPSGSLNGLFRVRSFTQDDAHIYCRKDQLQSEI
                     KRLLKLYDEVYKTFNLSYHIELSTRPEKKYIGDLKTWNLSEKALAKACIASGIPFKIN
                     EGDGAFYGPKLDFKLRDSLGRIWQCGTIQLDMNLPERFDISYIDANNQKVRPIMLHRA
                     VFGSIERFIGILIEHYAGAFPLWLSPTQIAVLPVNNDAHLKHAKSVVRKLEKQGLRVE
                     LDDSNEKLGYRLRNSQINKIPYTLVIGDKERNDNLVTYRKFGDKAQITVPYREFITLI
                     KREIRDKA"
     gene            24110..24721
                     /locus_tag="PLU08_01625"
     CDS             24110..24721
                     /locus_tag="PLU08_01625"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOQ70442.1"
                     /translation="MAFTLKPLPYAYDALEPYIDAKTMEVHHSKHHATYVANLNAALA
                     KDPSFNDWSLEKILKNLDKLPESIRTAVRNNGGGVYNHDLFWTLLKVNNGKGPEGKLK
                     DALVATFGTFEAFKDAFATAAKSRFGSGWAWLVVTSDKKLKVVSTPNQDIPFDEGTPI
                     LTLDVWEHAYYLKYQNRRPEYVEAFFNVINWDEVARLYENALK"
     misc_feature    24773..24885
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            24894..25445
                     /gene="infC"
                     /locus_tag="PLU08_01630"
     CDS             24894..25445
                     /gene="infC"
                     /locus_tag="PLU08_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOQ70443.1"
                     /translation="MLIYNQNRERPERNTDLVNENIRFPEVLVIGPNGEQLGVMKTRA
                     AIDLAASEYELDLYCVNPNGKPPVCKMLNYSKHRFEQQKRAKEQKRKQKTVELKEIRM
                     SPVIDVHDQETKGRMARRFLEDGNKVKVTIRYRGRQMAHIDVGEKILESFVASLEDYA
                     IVERKPLMEGRNLSVILASKIKK"
     gene            25457..25651
                     /gene="rpmI"
                     /locus_tag="PLU08_01635"
     CDS             25457..25651
                     /gene="rpmI"
                     /locus_tag="PLU08_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOQ70444.1"
                     /translation="MPKMKSKSALTKRIKVTKSGKLMRKAAYTSHLAPRKTTKQKRQL
                     RKARLVHKSDAKRIKYLIQN"
     gene            25664..26023
                     /gene="rplT"
                     /locus_tag="PLU08_01640"
     CDS             25664..26023
                     /gene="rplT"
                     /locus_tag="PLU08_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOQ70445.1"
                     /translation="MARVKSSPVTRARRKKVLKQAKGYFGAKSLLFRTAKEQVMHSLR
                     YAYIDRRRIKRDYRKLWIKRINAGCRMCEISYSQFMHGLKLANVDINRKMLSELAIND
                     FETFKGLTETAKKALAK"
     gene            complement(26101..26427)
                     /locus_tag="PLU08_01645"
     CDS             complement(26101..26427)
                     /locus_tag="PLU08_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70446.1"
                     /translation="MNQSEIMKNLKQEIKSSYEGFLKLGIHDEQAFHEHKEAIAFSVH
                     TVKAIAKILEPIPNEILYFKGGIGDTLAAVKEYAKNLVSKFGTLNTYKLYDFMSIDLP
                     TLRDTL"
     gene            complement(26428..26694)
                     /locus_tag="PLU08_01650"
     CDS             complement(26428..26694)
                     /locus_tag="PLU08_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70447.1"
                     /translation="MGRLTTKLPNILRVFDMGDALAFKEEEHYYIIIKRRSHLPIYNW
                     RYFELVIRSVCEIKNMTLISALEIEDNPFKAYFIEKGTLIKRAI"
     gene            complement(26707..27879)
                     /gene="thiI"
                     /locus_tag="PLU08_01655"
     CDS             complement(26707..27879)
                     /gene="thiI"
                     /locus_tag="PLU08_01655"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOQ70448.1"
                     /translation="MLMIHFGELYTKGKNRRDFIRQLTNNIKEVVANFKVQIETRHDH
                     IYIKNIKEKDVAEITKRLQNVSGIHAISEVIEFPLDLEKVKQFALELILELKPKTFKV
                     ITKRKDKLFPLTSDEINRSVASHILKNAEIKVDVHNPELPLRLEIDKSRILVVGKRYD
                     GLGGYPLGAAGKGLVLLSGGIDSPVAAYLMIRRGIKIAAIHFAAPPYTSEEVLKKIEA
                     LTAKLNNYQPHIRLYVVPFTKLQLAIYEHATSGYAITLMRRTMLRISTVIAKRHGYKV
                     LVTGDSLGQVSSQTLESLVVINEVTNYPIIRPLATLDKNEIIKIAKDIETYPISILPY
                     EDCCTIFPVTNSVTSPKLEDAVLFESKFDYESLISEAIRDTKKVDIRLDDGKEDLF"
     gene            complement(27881..29014)
                     /locus_tag="PLU08_01660"
     CDS             complement(27881..29014)
                     /locus_tag="PLU08_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOQ70449.1"
                     /translation="MIYFDNASTTKIHPEVLRTYIEILEKYYGNPSGMHTFAREIARL
                     QDKSREEILRMFKFRDGKVIFTSGATEANNIALIGLYEAYKNRGNEIIISDYEHPSVR
                     AACEYLEKERGAIIHKVPIRFKGGFDFDVFKSLLNDKTVLVSVMAVNNEVGDVIDLGE
                     IRACLKKYPKVIFHTDVTQALGKLDFHPNAADAYSFSAHKIGGLKGSGALLLRKNLKP
                     KPLVYGGSQEEGIRPGTHNAPANIVLAKTVRIAEGNMDKFYDHSYRLALKLHNFLATH
                     KDLFIINSVGGSNPYIVNFSLKTIKASIVLNALESRDIYVGTTSSCSAKLHAMSKSVY
                     AISQDEERASNSIRVSFGMENTLDEVEIFCNTLTEIIKEYGRE"
     gene            complement(29011..30726)
                     /locus_tag="PLU08_01665"
     CDS             complement(29011..30726)
                     /locus_tag="PLU08_01665"
                     /inference="COORDINATES: protein motif:HMM:NF017926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005940 - septin ring [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HOQ70450.1"
                     /translation="MKVVTLLNTLDIVFIVASIILVLAVFGFILSKFAFRGTKNKKAL
                     KSLSIKYSYVRSLLLGENIQYIKRIEAISDINLLMRPAYEEFFTRFEQLRNVDDRKAS
                     RSIQILNEALAAGNKEFKKAFNEHRPIILDYERKVTALSKDLTNMIKPESEIREESVY
                     DKEKLRIIKNIYRDHKDELEICVPSFEKIFTQIELLFKEYDNALNGAYYDEARSYLEK
                     IRTFNDQLERILQILPLLVVKATVLIPEKIATLKKSYEEATAYNVPLHHLRVSAEIES
                     FNEQLEVIKEQLIRFDTRNVEQTLNQIADTIDELINSFNVEIEKQREFTAEYDKTYLH
                     VNELEQRYIRLVNRMPQVKEYYLINPEYEKKLEEANTNVNTMMMVKRSLDAFLHSATK
                     QPYSVLVEKVQQLQAESMKVEVLVREFQTYIESLRVIVEDGFAICKLLFIRFKELEVT
                     LRKLNVPAYIQNFEQDFDSGYAMISDISTIIRERPIDVSQVEVLTNELRALATRLEDV
                     ITTDVKYAHSAEEVIVSLNAFRPQFSELHSKLRDEEQRFFNGLFKHTYENSIEQLKKY
                     QNRKQ"
BASE COUNT         9888 a         5630 c         5569 g         9755 t
ORIGIN      
        1 tactattgat aaaaagccag ggtattccgc aattccgcgc ggaacacgtt ctattaataa
       61 tgaaccataa taattgttaa aaaaatataa aacatccgtc gcgggaagtt ttcccatttt
      121 acatttgagt tttttattta acttatcact tacaccagca tttgttggtg gtggtattga
      181 ttctttcttg gtgtagaacc attcttccgt gtagtaattg tcacgcttgt aaagtggctc
      241 aaaaacattt tcatcatctt cactaggttc ataatgaata taatttacgg ccatttttag
      301 gtcatcgctt cctatatcgg tcgcaattgc ttttacatct agtgaaaaat gacttacttc
      361 actttcagtc gttttatcaa ggttataaac tacttttcct cttaatactt ccccaatata
      421 tgtaaaattt acagaatcaa aattatggaa ttgtgacttt tctttcgccg caaataccgc
      481 tttattagct ttttgtttag atactttaat gccgctatcg cagctagtaa taactaaaaa
      541 gataaaagat aaaggcaata atttcttaag tttcataact catactccat aatttattgt
      601 accactattt attctataaa taaaaagtac cgaagtttta ccctcggtac tcttacactt
      661 taaattattt aactattcgt atttagtcgc ttttaagcgt tcaattaact tactatcaac
      721 gcctttaatg accgcgacga gtaaatcaat agctgcatca atgtcatcac tatgcacaag
      781 tgagacatga ctatggaaat agcggcacgg tagagatagc gtcatattaa tgacaccgtc
      841 atgcacctta tgaatttccc cactatcagt gccaccagca gttaacatat cgtaagtata
      901 tttaacgcca atttcttttg cgacactttc aacatggtca aaaagtccgc gatggccaat
      961 cacgctgccg tccataatgc taagcgctac tcctgctcct agttttgtgt ccattgctgg
     1021 tgacccaggt aagtcgtaac tcattgttac gtcaagggca aaagctaggt caggtttaac
     1081 tttgtatgca ctagtgcgtg ctccgcggag tcccacttct tcttggacag taccggcaaa
     1141 actaacattt gcggtatggc cttctttttt caggcgacgt aaaacttcaa tcccaattgc
     1201 aactgcgaca cggtcgtccc acgctttccc taaaagtgtt ttaccatcat ttaatacccg
     1261 gaaagtttgt aagggtgtaa cagtgtcacc aactttaatt ccaagttttt taatgtcttc
     1321 gtcactaccg acacccatat caagatacat atcttttagt tccatcactt tattgcgttg
     1381 ttcagcgggc atgccgtgcg gaggttgagc accagtgaca ccaatatatt ctttaccatc
     1441 acgcgttgtc acgacccatt cgtgggctgg gataacgtga ccccaccagc caccaacatt
     1501 atggagccgt aaaaagccac ctttttcaat ttgtttaaca aggaagccaa cttcatccat
     1561 atgcgcagcg attaagacac ggggtgcgtc ttcttctcca tttaaatttg cgataactga
     1621 acctaggtta tcgtattcaa aagaatctgc aacatctttt aattctgctt taataagacg
     1681 agcgacatcg cgttcagcac cgctgatgcc tttgacttca cttacttttc ttaataattc
     1741 taaacgtttt ttatccatca tgacctcttt ctattacttc tagtaattat gttgttgtct
     1801 gtcatagttt accactagtt tagcgttata ggcattagtt atgtcttggg cactaaactc
     1861 taaagcgcta ccaagactta aaaattcttt taaagcgact gtgtaacagc gtttactttc
     1921 tttccgcatt aagtacgtaa tccgtaaata aacacgctta aaggcattat ttacttcctc
     1981 tttagtcgcc ggtaaacttt cgtactctac tttaccgact gcaaaattaa agacaatcgc
     2041 aattgagagg aaaaaatgta aaccgtccgc gaattcatca agtagtacat cgcgactttc
     2101 acttttttta agcgaccaat atttaaaggc acgcgttgca ttagcgagtt cacctatttc
     2161 cacaaggagc gctaaaaagc gctccgtttt agtactttcg tgagtgacat tatgacgact
     2221 atttatatca gcgtcaagtt cacgttgttt aaggagtaat ggtgcaagat taatttccat
     2281 tttttacctt gtcaatatgc caaatttcat cgatatattg atgaattgtg cggtcactgc
     2341 taaagaaccc gctcttagca atattataga gcgcactttc cgcccagctt gatttcttcc
     2401 gataatacgt gacaatttcg tctgtgcgtt taaagtaggc tggtaaatct tttaagatta
     2461 agtactcgtc acccttattc attacttcgt caaatattgc tttgaaagta tgggggttac
     2521 cttgactcca tgtaccgtcg attaatgaat ctaatatccg acgaatttca tggtcattat
     2581 ggtaaacatc ccacggatta taagtacctg attctctaag tgctttcact tctggctcag
     2641 ttaagccaaa gataaaggaa tgttcgtccc ctacgcgctc atgaatttcc acattcgcgc
     2701 cgtctagtgt ccctaaagtt aaggcaccat tcatcataaa cttcatatta ccagtgccgc
     2761 ttgcttcttt cccagcggta ctaatttgtt cactaatatc ggtcgcagga ataataattt
     2821 cggcagtctt gacgccatag ttttcaataa agacaacttt cataaatggc gaaatttttg
     2881 gatcattgtt aacgacatca gcgacaccgt taattaattc aattactttc ttcgcaaagt
     2941 agtaacttgg tgcggctttc gcgccaaaga taaaggtgac aggatgaatt ctaaaatcag
     3001 catcactctt catccggaaa taaagcatca taatgcgaaa tacattcatt aattgccgtt
     3061 tataagcgtg taaacgttta atttgcacat caaaaatcga atcaacatca attttaatgc
     3121 cgttagcgcg cttaatatag gcggcaagtt gttgcttttt gaggcgttta atttgtaaga
     3181 atttccgttg cactttctta tcttttacaa aaggtaagag tttttcaaag tcatcgggat
     3241 tagtaatata agagtcccca ataagacttg ttaaatattt attaagttcc gggttacttg
     3301 ccacaaacca gcgacgatgc gtaatcccgt ttgttttatt attaaatttc tcaggataaa
     3361 gactgtagaa ttctttaaat gtttgcgctt ttaagatttc agtgtgtagc gctgcgacac
     3421 cgttaacact aaaactagcg taaatcgcta agtttgtcat atgaatcatc ccgtcgcgga
     3481 taattgccat attttggcga tcaccttcac tgatgccttt attaacgaat tcgcgttgac
     3541 tacgacgatg aatttcttca ataatcatgt agttgcgtgg cactaaatga tggacataac
     3601 gcactggcca gcgttcaagt gcttcgacca tgattgtatg gtttgtatag gcaaaagtct
     3661 tagtagcgat atcccacgac tcatcccagc catattcata atcatcaagt aaaatacgca
     3721 ttaattccgg aattgctaaa atggggtgcg tatcattaag ttggaacaca tagtgttcat
     3781 ggaaattacg taacgtttta tgacgacgga aatgaccgcg aatcgcactt tgcaagccag
     3841 cactgactaa gaaatattgc tgtcttaagc ggagcatttt acctttttcg gtagaatcat
     3901 caggatataa cccgcgatta atttcgcgga gtgtccgtaa ataatcatca aatgattcgt
     3961 cacgtggtaa ttgttcatca cttggttcag cgctccataa gcggagagta tttgcgacgt
     4021 cattacgata gccgacaaca ctcatgtcat agggcactgc tttaacgata gtgggattaa
     4081 cagttctaat cccaaattca ccattgcgtt tttgataagt ttcagcgtga ccgtaaaact
     4141 taacattcac cgagtgcttt ggtttccgta cttcccagac gttgccattg actaaccatt
     4201 gatcgggtaa ttcgacttgt ttcccgtcga caatcttttg ccggaagaac ccgtattcat
     4261 aacgaataca gtgaccgttt ccagcgtaac caagactcgc aattgaatca aggaaacatg
     4321 cggcaaggcg acctaaacca ccattaccaa ggccagcatc actttcttgt tcttctaaat
     4381 catgaagatt catcccaatt tgttttaggc ctttgttaac gacattataa attcctaagt
     4441 tgattaggtt gtttgtcagt aagcgaccaa ttaaaaattc cattgagaaa taaattaatt
     4501 gtttcttacc tttcttatta acagcgtcac ggcttttgcg ccagttgatg ttcgcgtagt
     4561 ctcttaccat tgaacctaaa atgtcataac gttcagtgat gtgactatca tcaaggccaa
     4621 cgccgtattt ctctgttaat cgctttttaa attcatgaat aaaacttttt gttgttgtaa
     4681 acataaatta ttccttcctt tatttattta actcactaaa ttatttagtg tctttttttg
     4741 ttttagcagt agttgctttt ttcgttgtag tctttttggc tgtagcactt tttgttgctt
     4801 ttttagtagc ggtgctcttt gtggcagttt ttgttgcctt aggttcagcc ttttttgttg
     4861 tcgctgcttt tttagcaggc gcttttttcg ctgctggttt ctttgcttta acttcttcga
     4921 cctttggttc ttctttcttt gcgggtaaat ctttctttaa aatcatcgcg gcaaatgaac
     4981 caagtttaag tgttaagcgg tatggacgca tttctggtcc tggaccagcg tgtgttgtca
     5041 ctggtaaacc gttatattgg ttataaccac catagacatc tttatcacta ttaaagattt
     5101 cagtgtaagt accttcttct tcaacaccga tatcgtaata ttcgtaataa tttccagtca
     5161 tattaaagac aaagattaat gtttcttcac cgtatttacg ttggaacgca aacacacttt
     5221 gatcacgatt atcgaccatt aaccatctaa agttacgagg gtcaaattca ccaacatgca
     5281 tcgctttttc actacgataa attaagttta agtcgcgcac taagcgacta actgaatcgt
     5341 gttttggata tgactttaag ttccacggta aactcttact ttcattccac tcatcgaagc
     5401 tagcaatttc gttacccata aaggtaagtt ttttaccagg atgcgcgaat tgataagtat
     5461 ataagttacg cgttaaggca aacttttggt catagttacc aaacatctta ttaataattg
     5521 tccctttacc gtgcactact tcgtcgtgac ttagtggtaa cataaagttt tcactaaaga
     5581 aataatgcat cgagaatgta aagaggttat ggtcatattt cttataaatt gggtctttac
     5641 tataatagcg taaggtgtca ttcatccacc ccatatccca tttatagtca aagcctaaac
     5701 caccccactc aacgtcccgc gttgtaccgt caaacgctgt tgagtcttca gcaatcatca
     5761 taacaccagg gaaatggtaa tgaactttac cgttgagtcg ttttaagaat tcaacaccgc
     5821 cgtgatttac tccgcggtca ctattaccgt gccagtagac aatgttactg acagcgtcaa
     5881 ttctaattcc gtcgaaatgg aagtattcta agaaataaaa aatcgcacta attaagaagc
     5941 tacgaactgg atcgcgccct aaatcaaatt gcggggaacc ccattgacta aatgtatgct
     6001 cactgtcttg atattcatac attcttgtcc catcaaattc aattaaacca taatggtcaa
     6061 tggcaaagtg aacagcaaca aagtctaaaa taacgccaaa accggcttgg tgcatacggt
     6121 cgacaaatga cataagttgc attgggttac cataacgact atcaacacta aagaaaccag
     6181 tagcctgata accccatgac ccgtcaaatg gatattgtgt aatcggcatg atttcgacat
     6241 gagtaaagcc attatcacgc atataaggaa ttaagtaatc agcgatttct tcgtaagata
     6301 aaatccgccc attttctttt cctttccagc tgcctaaatg gatctcgtaa atactaattg
     6361 gtttatcatg acctctacat ctacttttca tccaatcttc gtcgtgccaa ataaaacctt
     6421 cataatcaaa tagccgtgaa gcagtacctg gtgctttttc actaaagaaa gcatatgggt
     6481 cagctttatc tacataatga ccatgggcat tccggaatcg gaatttatag ttttggtaat
     6541 tttctaattc aggaatagta atttcccaaa taccacggta atcgaccttc tccatcttgt
     6601 ggacatttgg atcccaaccg ttaaaatccc caatcacact tacatcactt gccgctgggg
     6661 catagagacg aaatgttaca ctttttttct ttttaccttt cgcgggatta aaatgagcgc
     6721 caaaatattt atgtgcactc gttgtaaacc ccatcaggaa ttcatctata aaactcataa
     6781 tcaacctccc tcgttttatt attataataa aaaaggacga agaatggtag tttaaacacc
     6841 ggaattatcg caattttgac agaaaatgaa aatttaccga caaatttaga cgaataatat
     6901 tcaatttatt gaaattttat gcattatcgg tattttttaa atatttgagt tattacaatt
     6961 ttagagttgt tactagctta ttgccaaatt atttatcgca aaatattaat ccgcaaacaa
     7021 gataaaaaga taatttgctc ttattattaa taatataggg gaaataatta atcaactttt
     7081 ttgtaacaaa atcacagcac gctaacttgt ctttgtgtat aataataaaa tgagaggtga
     7141 aatatatgtc aaaattagtg ttagctatta actcgggaag cagttcctta aaattcaaac
     7201 ttttcaaagt tccaagtgaa aaagtaatta caagtggact ttttgaaaga attacacaaa
     7261 aaatgggagc gtttcgcatt aaaaccgaaa cgggtgaagt aagacgcgaa ctaccactac
     7321 caaatcacga agtagcggtt aatgttttaa cgaaggcttt aattgaattt aaaatcgtta
     7381 aatcattaga tgaaattaac gcagtgggtc accgtgttgt tcaaggcggt aaatattaca
     7441 atgatagcgt tgaatttacc gatgaagtcg cggcaatggt ggagaaactt agtccttttg
     7501 ccccacttca taaccccgca aacttaacgg gctacgaagc ttttaagaaa atcttgccag
     7561 atgcactgca tgttgctgtc tttgatactt cttaccatca aacaatgcca ccagaagatt
     7621 atttattccc cattccttat gaatattatg aaaagtatga catccgtcgc tacggagcac
     7681 acggtacttc acataaattt attgctgatg aagttacaaa aattaataaa aggaaaccgt
     7741 gtaatcttat cgtctgtcac ttagggaacg gcgcaagtat tactgctgtt aaagacggaa
     7801 aatgtgttgc tacatcaatg ggctttactc ctttaggagg catcatgatg ggtacacgaa
     7861 ctggtgacct tgatccaagc gtcttttatt ttgttagtaa attagaagat aaatcacccg
     7921 aagaagttta tgacatgttt aataaaaagt caggaatgct tggtgtcagc ggtatttcta
     7981 gtgatgcacg cgatgttgaa aatgctcttc ttcaaggcaa tgaacgcgct aaacacacct
     8041 ttgatttata tagtcggcgt gtcgctgatt atattggtca atatcatatt cgtttaggtg
     8101 gtactgacac cattgtcttt acggctggta tcggtgaaaa tgtcccaggc attcgtaaac
     8161 aaattatcga caaagttaaa gatgcgatgg gcattgaatt tgatgacgaa caaaatagct
     8221 taactagagg tggaatttat ggtttacttt ccacgaaaaa tagtaaagta aaagtatatg
     8281 tgattccgac tgatgaagaa ttaatgattg ttcgagattg tgtgcgaatc gctgaaaata
     8341 aaaagaaaaa ataggtgtaa ttaatgcaca tatattcaaa agaaattgaa cttaaaatca
     8401 aagagtataa aaaaacaacc aaagcaattt ttagtcttgt tgaaaagtat gataaggtag
     8461 ctattttcac gcacctttca ccagactttg acgccttcgg ttctcaattc ggtttaggag
     8521 tatatttacg cgaccattat cccgagaaag acatcaaaat tattgggcaa agtttaccac
     8581 tttataacgg tgaactttat cccgaaatgg atgaagtaaa ggatgaatgg tttaatgatg
     8641 attttttagc aattattgtt gataccgcga cgcttgaccg tgttagtgat aatcgttatg
     8701 aaaaagctaa ccatctcatt aaaattgacc atcatccaaa tgttgatccc tacggtgata
     8761 tatgttttgt tgatgttact agtgttgccg tcgccgaaat gattgccgtt atgcttttag
     8821 cagatggcgg cactatatca aaagatgtcg ctagttatct atttagtggt atcgttggtg
     8881 attctggacg ctttaaatat gcgtccacta cttcacttac cttcggtatt gccgaaatct
     8941 taacaagttt aggcattgat ttaaattata tatacaatcg tttatacatc caaaacttag
     9001 atgacttacg ctgtattggt tatatcttaa ataattttaa aattacgaaa aatggttttg
     9061 cctattacgt gatggatgag gctacactgc aagaactcaa tattactgct gaacgcgcaa
     9121 aagaaaatgt taatgttttc gctgatattc gcgggattaa agtctgggcc gccattaccg
     9181 aacgcactga cacaaacgag tggcgggtat caatacgcag tgctggtata ccgattaatg
     9241 aattcgccca aaaatggcga ggcggtggcc atgtgcaagc tagtggtgct tcacttttaa
     9301 accgtgaaga aattgatgta tttgtgaaag aaatggatga atatataggc taataagcct
     9361 attttcattt cttgtctctt caatatgtta aaataattat atgtctttta tccctttaaa
     9421 tgtatttaca aactatgcgc tccttcaaag tgcacttact ttggacgatt atttaaagac
     9481 acttaaaaat cgaaacatca aagcagcggg tgttagtgat cctgataccc tttttagtta
     9541 tccgcacttt gccaaaaaaa gtagggaatt tcatataaaa ccactctatg gtgttaggct
     9601 taattttgaa ggttcttcac tagttattta tcctcaaaac gaaaagggat atcgtgaact
     9661 tttagcgctc cttgaagtca aaaataaacg cgaattgacg ctgcaagatt taaaagaaaa
     9721 tagcacaaat ttaattgtta ttattccctc ttccgctatt cataatttag agttaaaaga
     9781 gcaaattatg caaaaatatt tttataattt tgccattaat acaacatcat tatacatcgg
     9841 tttagaacgg gaaaatgcag aattaacatc ctttttgcga gaatttagtg ataaatataa
     9901 ctatgcacta gtagcgttcc cgcttattaa atatgctaaa agtgaagacc ataaaacact
     9961 tttaatgatt gaggcaatta gagaaggtaa atcgttttcc atttatgata tttctagtgg
    10021 tcccgattat attttaagtg acgctgaact taataattat tatagtgaaa atgaacttaa
    10081 aaatacgcat ctaattagtg atttaattaa ctttgattta agcgttaaac acgacttaaa
    10141 tttcccactt cctggcgcat atgatcctaa taagaaaatt gatgaattga cactaaatgc
    10201 gcttaaagca aaaggcttag ataataataa aaaatacata gaacgactca cacatgaatt
    10261 aagtatcatt cgtaatttag gctatgttaa ctacttctta atcgttagtg attatgttaa
    10321 ttttgcaaaa aaagctggaa tcttagtcgg ttacggtagg ggtagtgcac cgggttcact
    10381 tgttagttat cttttaggta tcacccacgc tgatccactt gaacacaatt tactttttga
    10441 acgcttttta aatcctgaac gtgtctcttt gccagacatt gatatcgact ttatggatat
    10501 tagacgcggt gaagtaattg attatattaa aaagacttat ggtgaagata aagtcgctaa
    10561 tattgtgact ttccaaacta atgccacaaa agcatcgttg cgtgatacag gtaatgtttt
    10621 taacattgat cccaaattta ttaatcgctt aagtaactct ttaggtaaca ctaactataa
    10681 cttccgtgat gcttatcgta acattaaagc attccgtgat ttagtggaca acgatactta
    10741 taacttagaa attattaaaa atgctactaa aattgaagga tttccgcggc aaagtgggct
    10801 tcacgctgct ggtattataa ttgacggcga accgctcgcg agaaacttac caacattttt
    10861 taataacggg ttacgcgtta cacaatatga aatgggattc cttgaagaac acggattttt
    10921 aaaagttgat attttaggac tatcaaacct tacctttgtt cataacatca ttaatttagt
    10981 gaaagaaaat cataatatcg acattgattt ttataatttg aaatttgatg atccgcagat
    11041 ttatgatgta atccgtaatg gtcatacatt aggacttttc caacttgaaa gcgccggaat
    11101 gcggaaagca attgcgacaa ttaaaccgac taattttgat gatgttagcg ccttacttgc
    11161 gctttatcgt cctggtccga tgaaatatat tgatgaatac gctctgcgta aggaaggaaa
    11221 gtcaccgata acttacatca atgacgactt aattgatatc ctaaaaccaa catacggcat
    11281 tatgatttat caagaacaaa ttatgcaaat tgccgcgaca atggctggct ttagccttgg
    11341 gcaagccgat atcttacgac gggcgattgg gcgtaaagat gaaaaaatca tcctagagca
    11401 aaaagatgca tttttcgctg gtgctaaaaa gcgaggctac tcaagaaaag ataccgaaaa
    11461 agtgtttgat gatattttac gctttgctga ttacggattt aataaatcgc actctgtagt
    11521 gtacgcgatg ctcactactg ctttagcgta tcttaaaact tattatcctt atgaattcta
    11581 tcaagaaata ttacgcggtt tagtaagtga taatgttaaa ttctccttag cgagacaaga
    11641 attaaaagca cttaacatca agataagtag gccttgtgtc ttaaaatcta caactgaatt
    11701 tattcgcact aataactcga ttaatttccc acttagtgcg ataaacggat taaacaggga
    11761 atccgcgaat atcattatta gattacgtga acaaaaagct tttagtagtg tgcaagactt
    11821 cttcttccgc agttttaagg ctggtgttac cgctgaagaa tttatcgccc ttattgaagc
    11881 tggggcactt gactctttta acactaatcg cgcacagtta aaagtaatgt ttaacaccta
    11941 cttaccaact ttagacattg gcttatttga taatgaagcg gctttacatg cgattattgt
    12001 tcctaagata aaagagagtg acgaccgtct tgatttagaa ataagtaggc tgcgcttccc
    12061 gctttcaggt aatccgcttg ataaacttaa actaaaagac attaaacgcg ttagtcatat
    12121 tttagaatta gaagacggtt acattaaaac ttatggtgta ctaacatcat tacggcttat
    12181 taagacgaaa aagggtgacg agatggcttt tgcgaccgct agtgattatg aagaagacat
    12241 taatcttgtc ttctttccgc gtgtacttgc ggaagttaaa gggatgttaa cccgcaataa
    12301 tatttatatt atcgctggta aacttgaaac cagggacggc caaaagcaac ttgtagttaa
    12361 cactttagaa agggtccaaa atgagtaaac gacttattat tatagatgga aattctttgc
    12421 tttttagagc atattacgct actgcatata tgggcacaga taaaattatg cgcacaagca
    12481 ctggcgtacc gacaaacgcc atttttgctt ttgcgaatat gattgtgcgc attgttaatg
    12541 atttaaaggc tgacgatcat ttatttgttg ccttcgatac cggcgaaaaa acatttagac
    12601 atgaagaata tgatgattat aaggctaatc gccaagcagc acccgatgaa ttagtaactc
    12661 aatttccgat tgctcgtgaa tttcttgatg cgctttcgat tcgtcacgac gaagttgtgg
    12721 gatacgaagc tgatgactta gcaggaagtc ttgctaaagc agcaggtaaa gaaggtttta
    12781 aagtcgaatt atacactagt gatcaagatt atttacaatt aattgatgaa gatatcagcg
    12841 tgcagcttat tcgccgcgga ctaagtgacg tgcgcttttt agacccaaaa ggactatatg
    12901 aagaatttgg ttatcacgct tatcaagtac cagactttaa agggttaacg ggggacgcaa
    12961 gtgataactt aaaaggagtg ccaggcgttg gtaaagttac cgcaacaaaa ttacttgccg
    13021 aatatgacac tttagaaaaa atacttgaaa atgccgacaa tattaaaggt aaacttgggg
    13081 aaaacattcg agaacataaa gctactgcct tacagtcaaa acacttagcg acgattctca
    13141 ctgacttacc gctaccgtgt ccgcttaatg acttccatta taaaggcttt gacaatagtg
    13201 ctgttcacgc ctttgcgcaa aagtatgaaa tgcgccaatt tttaaataac ttaaaatata
    13261 caaatagtga agttgaagaa gaaattaaag ctcatgaaat caaaagtttt aatgaagtta
    13321 aagaaaaaga ttatagttat atttcctttt atgttaccgc tagtgatgaa gattattaca
    13381 aggcgacacc agaatttatt tatttctcac ttgggaaaaa tgtatatcga ataagcttta
    13441 acgatttact tcttgatcca aagcttaaag ctttatttag tgatgaagca cttcttaaat
    13501 atacttatga ctataaacgt catgccatta tgctaagtaa ccacgctcta gatataaagg
    13561 gcacagttaa tgacgcactt gtcttaactt atgtatggga cacgaatgtg gcacctaaca
    13621 tcagcgcttt cttattaact tacggggtta acgctagtag tgaggaagag gaaacttacg
    13681 ctattttagt tgcgaaatta agtgctattt atcaagaagg cattaataaa cttaaaaaag
    13741 aaaatttgct ccaagtttat gaagacattg aaaaaccact gactgatgta ttaataaaga
    13801 tggaaaaagc cgggtttaat gttgataaag atgtgttaac cgcaattgga agtgaatata
    13861 aagtcattct tgataactta acgcgtacaa ttttcttgct cgcaggtcat gaatttaata
    13921 ttaattcccc aaaacaacta gcgacaatcc tttttgatga attggggctc aaggctaatc
    13981 gtaaacgctc cacttccgct gatgtgttaa atgacttaaa agataagcat ccaattattc
    14041 ctgctattct tgaataccgc aaatatcaaa agattatttc aacttatatt gatggcttta
    14101 ttccccatat tcatcgtgac gggaaagttc atactacctt taaccaagcc ttaacgacaa
    14161 cgggtcgact aagcagtaat aatccaaact tacaaaatat tagcattcgc catgaagagg
    14221 gacgcgagat aagaaaagca tttaatgccg gagataagga tgttttagca attgactata
    14281 gtcaaattga gttacgctta cttgccgcta tcgctaatgt tcctagtctt attgatattt
    14341 ttaacactga ccgcgatatt catagtgaaa ccgcaatagc actatttccg cttgaagact
    14401 taaattacgc aagacggaaa gcaaaagccg ttaactttgg catagtgtat ggcattagtg
    14461 attttggctt aagcgaacaa cttgaaacaa cagtaaaaga cgcccgcgct atcattcagt
    14521 ccttcttcaa gacttatccc gaggtatgga cttatagaca agaagtaatt aatgagttaa
    14581 ttaaggatgg ttacgttaaa acattaactg gtcgtaaacg ctatatcccg caaattaatg
    14641 atcctaacta tcaaacacgc gaatttgcaa agcgtgccgc gatgaatgct ccgcttcaag
    14701 gcagcgctgc cgatttaatt aaaatggcaa tgattaaagt cgacgaattt ctaacaaaga
    14761 ataagtgtaa aacaaaacta attttacaag tccatgacga acttatcttt gcgcttgatc
    14821 aagatgaaca tgaaattgtc gacgatttag tgaatattat ggaaaatgtc cttgatttac
    14881 cagtaaaatt aaaagtggaa gttaaaaccg gaaaaacatg gtttgaagcg agttaataat
    14941 tatgccagaa ctaccagaag ttgaaacagt taaaaaaatc ttaaataaag tcattgttgg
    15001 ggcaaaaatt actgatgtta ttattcataa agaaaacatc atcactgggg acccacacac
    15061 ttttaaaaag acacttattg gtgaaacttt tagtgaagta aaacgcattg gtaaatactt
    15121 aatctttacc tttgaaagtg atttaattat gcttagtcac ttacggatgg aaggtaagta
    15181 tattgaagtt ttaccgggta ctgaagattc gcgttacgct cgtgtaattt ttgtgttaaa
    15241 cgatggcagg cgcttgtgtt atgacgattc acggcaattt ggtacgcttg aattatcaac
    15301 taaagacact tacttacaaa caaaatcaat gcaaaatgta ggaagagaac cctttgatat
    15361 tgatccgatc gattatttta ataaaataag taaccgcact acaccaatta aacaaacttt
    15421 acttgaccaa aagctgatga gcgggctcgg taatatttat gttgatgaag tattatactt
    15481 aagtaaaatt catccagaaa caccaacgaa tatgttagga cttatggcaa taaatttact
    15541 tatcgaaaat agtattaaag tacttaacgc ggccataaaa aaaggcggca caaccgtaag
    15601 aagttatagt cctgctgaag gagtgcacgg taacttccag caagaattaa atgcttatgg
    15661 taaggcgggt gaaaggtgtc ctgtttgtaa ttttaaattc aagaaaatat ttgttggtgg
    15721 gcgcggtaca acttattgtc caagttgtca aaaaaatccc gctgtgccat taactgtcgc
    15781 cattactggt caaaaagcaa gtgggaaaac acttatcgct aattacttac ggaaaaatgg
    15841 acacaaagtt attgacactg ataaactcgc taaagagctt tataaagata ttaaaattac
    15901 aactgattta gaaaaacatc tcggcgttaa tttacatgat aacactactt ttaaccttca
    15961 atatttacga aattatctag tggaaaatga aaatgaaata aaaaaactaa atgactttat
    16021 tcatccgctt gtaaaaaaag aaattattga aattattaag acgagtaatg aagcgattct
    16081 ttattttgaa gtaccgcttc ttttctcgca taaaatatat gaattattta attttatgat
    16141 tggggttgaa gtaagcgttg aaaagcaact taagaattta gaaaagcgcg gagatacgca
    16201 tacaattaac gctgatgaag catatatgaa aaaccgtcat aaactagact taatcatcgt
    16261 taatgacggt agtgaacaag atttaatcga aaagtttaaa gcttttaaag tttagtgcct
    16321 tgtaaataaa tttcttcttt aatattggct ttaattaagt caacaatttg atggccacgg
    16381 acactgcgac caacataaag acttcgtaag tcattcgtgc ttagacccgc taaaataata
    16441 gtgggctttt tttgtctgac tctcgctgct agaagtggaa aaataattga ttcaatgaca
    16501 atcttattaa cttcttcatt accgaagtta tcaagcacta aaacatctaa atcaatataa
    16561 tgttgtaaat aagaatcaac gatatctttt ttatcgtagt aatcagcaat tgatgctcta
    16621 acaaaagcag ggaaattgac aactcccgct gttttatttt tatcatcttc aagcatttta
    16681 gtcacaaacg gaatcgttgt ttttaattta ccaatccccg ctgtaccata agcatatata
    16741 aagtagatat caccttttaa aactttcata agttcacgaa taaaagctgc aaagcctcgt
    16801 ctagcgggta agtcttcgac tctcaaatcc aaataatttt cgggaaaatc acgaacaata
    16861 aagcgcatcg ctaatgattg actttctaga taaactgggc ataaatgtct ttccactgtc
    16921 acaaacggcg cgtcaatttg aatagtgagt gcatgatgct tttcatcact aacgcagtga
    16981 ccagctttac gacatttttc tacacttaag taactttcta aataatcatt taaaagtcca
    17041 acattgttct taattatttc tggagttgca cctttttctt ctaaaagttt aacgaaagat
    17101 ttatgactca taattttgtc atacatttct tggcgtattt cttcaacatt tgtggtgtca
    17161 atatctaatt ctactttttt cattttaact tcttcaattc atcccaaatt ggatcatcaa
    17221 gatcatcaac ttgcggtgtt gcttcttctt tcttgtcatc gttaaggatt ggcgcttttt
    17281 taggtgcgcg tccgcgtttt tttatcggcc gttcaagtgt atcaagggca tcaagcgcgg
    17341 tagttacttt gtttctcgct agtaaactag cgtttttctc cactaaagca cggataagtt
    17401 cattgttatt atgataaagc gtgtaataaa caagggcatt aattactggt gataataaat
    17461 tataatcatg acttagcgtc gttaaaatat tataatcagc aggagcaatt ttcgtccctt
    17521 catttaagta ttgtaaaaat tcaacgggac tatatgtttc cattaaatta atccgcttaa
    17581 ttttttgact ttcacccgca agttcttgaa ctggacggcg taatggttta cttaaaacgg
    17641 ggtacttcac aagttcttgc aagtgcttat tcatcgccac aaaatcgact ctagcgccaa
    17701 agggttgtag agcgtcataa aaatcaccga cacgctccgc tgttgctact tccgtcaaat
    17761 tatataaacc agccatttgc tcagcttttt taagctcttc agtacttagt gctgagggca
    17821 aaattttacg ttcacgcgtt aagatattaa caaaagcagc gcgatcaaat tcaaatttaa
    17881 tattaccgac aacattttct aatggttcac ccttatatag gtcatcacct ttaatatcga
    17941 aaatacgatc agcgtaaatt tcggcaaaag ttgatgtaac ttcttcttta tttaatgttt
    18001 cacgatcgag cgtaaataaa gataataagt cttgtgtatg gcgcggacca agcacatcat
    18061 ttaataaatt cataaagatt tgatcttgac taaacgctgc tgggtctttt ggtgcatata
    18121 attctaagac atattcacta aattcattat attcaacata aaatgaccgc attaaaccaa
    18181 gaccttctaa tttactaaag gcgatactaa tttggtcata actaaaccca aaatgatcat
    18241 gaattaaagt aaacgatagc ggatcacctt ttctaaaatc acgcattgta aataaatagc
    18301 gatataaggc tgtcgcggca aagcccatga ttggttgata taaaatatct aaaacttcaa
    18361 gatgtaacga tgttacataa ccgcgaactt tcacactata gaaatcatta cttttaatta
    18421 atcgcatagt aaaattatta taacacaatg aataacgcac cgttaaggta agtatgtatt
    18481 tttttacaaa gaacaaataa ttaccaaagt taacactttt ttgtagaata gaaagaaaat
    18541 attcgcactt tctttaaaat tgactattat aaataagtca aaggtattgt taacatgata
    18601 aaatataaaa tgtatggagg aagcaatatg acagttaaag tagcagtatt aacatcaggt
    18661 ggcgacgctc cagggatgaa tgccgcgatt cgggcagtta tccgtagcgg actccttgct
    18721 ggctatgaga tgtttgctat ttttgaaggc tttcgcggaa ttatcgaaaa taaaatagtt
    18781 agtgtcgacc gtactttcgt tagtgaaatt attatgcgcg gtggcactat tttaaaaact
    18841 gcgcgttttc ccgagtttcg tgattcaaaa gttgtcgaaa aagcagcgga aaacttacgc
    18901 ttctatggta ttactcatct aattgttatt ggcggtgacg gtagttatcg tggtgcatat
    18961 gctttaagta agcacggcat taaaattatc gggattcctg cgacaattga taatgatgta
    19021 atgggtactg aacaaacaat tggttttgat acagcgctta atacaattac ggaagctgtt
    19081 gacaaacttc gtgatacttc tagcagccat caccgctgtt caattattga agttatgggt
    19141 aatcgctgtg gcgacttagc gctttatagc ggaacggcat gtggggttga tttaattatt
    19201 tcccccgaat atagacctag cgaagaagaa attattgaaa cactaaaaaa cattaactta
    19261 aatcgcgcac gacacgcgct tgtgatcgtt agtgaaaaac aatttgaaga tttagaaaaa
    19321 ttgcgggagc gtatcgttga agaaacaggc tttgacgcta aaattgaagt gttagggcgc
    19381 attcaacgcg gcggctcacc gacggctagt gatcgggtac ttgccggtcg acttggtgta
    19441 tatgcgattg atttaattgc taagggtatt gacaatgttt gtgtcggctt agttgatggg
    19501 cgcttacgac atcacgccat cgctgaagtt actaaatcaa ctaatccaaa tttagatgaa
    19561 ttactaaatg taattgataa actcaaatag aaaggaacac ctttatgtta aatatttcca
    19621 agaaaactaa aattgtcgcc acaattggac cagtgacgaa tactcttgaa aaagttcgag
    19681 aattaattga tgccgggatg aatgttatgc gcatcaactt ctcacacggc acttatgaaa
    19741 cacacaaaac aattattgat atttccaaaa aactaatcga agaacatatt tatattccga
    19801 ttatgcttga tacagctgga ccagaaattc gttgtcactt ctttgaagat gggaaagttg
    19861 aaattgtcaa agactccatc gtcaccatca atatgaaaga agtattaggc accgctacag
    19921 aatttagtgt taattatcct aaattagtcg atgatattct cgtcggtgac atgattaaga
    19981 ttgatgatgg caagttaaga cttgatgtta ttgaagtcga tagagaaaag cgtttaatca
    20041 aagttaaagc atttaatacg catgttattt ccaatcgtaa aggcattaat attcctggtg
    20101 aacgaatgac tttaccagca ctaagtgaac gcgataaaaa agatattaaa tttggaattg
    20161 aaaacggcat tgacatgatt gcggcaagtt ttactaggtg tgctgctgat gttattcaac
    20221 ttaaaaatta tttagcggaa ctaggtgcac cggaaattcc gattatcgct aaaattgaaa
    20281 acaaacaagg tgttgataat attaaagaaa tcttagaagt cgctgatggg atcatggttg
    20341 cccgtggtga tatgggagtt gacattccac ctgaagtcgt tcctgtgatg caaagcgata
    20401 tcattttaga atgtcgtaag gtcggcaaac cagttatcgt tgctacacaa atgcttgact
    20461 caatgcaaac taatccgctc ccaacaaggg cggaagtaag tgacgtcgcg actgccatta
    20521 aagaaggcgc tgacgctgtg atgcttagtg cggaaacggc tagcggttta tatccggttg
    20581 aaagtgcagc aatgcaacaa cggattgcca ttactatgga agaattttta ccatatactc
    20641 aacttgcaaa ctttggctat aactattcaa agaaaaagcg cagtgacgcg attgccacaa
    20701 gcgtcgcctc aacggcgaat ttaatcgacg ctaaagcgat tgtttgtttt agtgaaacgg
    20761 gtaaaagtgc gatttgcata tctaaatcac gtcctaagtg tccgatcatt atggtcacga
    20821 gtagtcccgc ggcgggaatg cgagttgcac cttactttgg catttatagt atcgtggtaa
    20881 aaacactacc acaattaatt gaagatatgg aagcttttgc gctcattaaa atgcgacaac
    20941 ttgggttaaa accaaatgat aatatcatca ttactggtgg tacgccaact ggcactggtt
    21001 cgacaaactt cttacggatt gtcgccctta ataaattagg agatatttaa tgtttattat
    21061 tgcacttgat attggtggca caaatattcg cgcagcaatc gctgaccaaa acgggaaatt
    21121 acttgaggtt gtaaaagaaa aaacactgcg ccatgataaa gaaattttta tcacgcaagt
    21181 taaggggatt attacacaac ttgatttaag taaatatgat cctattgcta tttccattgg
    21241 tgtaccgggt cgcgttagac aaaacggatt tattgatgaa ttaccaaata ttggcattac
    21301 taatatcgat ttagttagtg ctttgcaaaa gacttttaag ctccctgttt cagtccgtaa
    21361 tgatgcagaa atggctgctt ttgctgaagc agcattagga gcgggtaaag aattaaaaag
    21421 cacttatttt gtcactatta gcactggcat tggcggctgc ctaattgttg accgcacaat
    21481 taaaaacccc agtccagaaa tcgggcatac actagtgcct tatcgcggta atttttatga
    21541 attagaaaag attgcttcag gtgacggagt aattaatctt gcagcgctaa atgagatata
    21601 ttacgcaaat gcccaagctt ttttcaaaga tgtcacttat aaagtgcctt atgccttaaa
    21661 aatctttgat gaatggttag atttgattac tgacttcttc atttttgtta atgaagcttt
    21721 tacgcctgat gtgattgctg ttacgggcgg agtattaaaa tcgaaaaaat tattttggga
    21781 caaatttatc gaacgtgtcc ctaacgctaa tattcaaatc gcgcatttta gtgaagatgc
    21841 gggattagtc ggtgcggcaa gttacggttt taaagtattt gcctcacagt aactacttct
    21901 tgcctattta ggcaaatatg tgtaaaatta tttaggcgaa gacaaagaca agagcttaat
    21961 gacttcactt atagagacct agtgggtggt gcaaactagg agggagcatt aatcttacca
    22021 ctttcgagcc tactaacgat gagttagtcg ctagtcacgt tacgactaac agagtgcatg
    22081 gcaacatgaa ttaaggtggt accgcgcaaa tatagcgtcc ttaaatgggc gtttttttat
    22141 taaaaaggag ggtatatgaa agaactcaaa tataacaatc aactagtaaa agttaatgac
    22201 ggcacaacag gttttgactt acttaaaatt ttaaacatcg aagggagaag taatgtttta
    22261 gtttataaac taaacggtga actttttgac cttgcaactc cagttgagca agatggtgaa
    22321 ttttcctttg tgacaaaaga ggacgaagaa gcttttgcga ttttaaatca tgacgctgcc
    22381 cacctcatgg cacaagcaat tttaagtatt tatcctggcg ctaaatttgg gattggtccc
    22441 gatattagtg aaggctttta ttatgatgtt gattttggtg atgttgttat aaacgatggc
    22501 gatttagcgg ttattgaagc caaaatgatg gaactctcta gagcagacca ccgtattgaa
    22561 cgccaagttg ttagtaaaga aaaagcatta gaaatctttg ctaaaaatga atataaagtt
    22621 gaacttatta atgaaattga ggagcgaatt actatttatt cgcaaggtga ctttgttgat
    22681 ttatgttacg gtccacatat gccaagtacg cgttatgtcc aacattttaa actgcttagt
    22741 ttagcaggag cctattggcg tggtgacgca agtcgtaaac aattaacgag aatttatggg
    22801 gtcgccttct tctccaaaga tgcacttgat aagcatctct ataaccttga agaagcaaaa
    22861 aaacgtgacc accgtaaatt aggtcgcgaa cttggactat ttatgcttag tgaatatggt
    22921 ccaggcttcc ccttctggct ccaaaaagga atgactcttt atcacgaact tgaacactgg
    22981 tggattaaaa aacaacgcga agcaggttac gaatttgtca aaacgccgca gatcttaagt
    23041 cgtgaactat gggaaacaag cggacactgg cttaattacc gtgaaaatat gtacacaact
    23101 cttattgatg aacgcgattt tgccattaaa ccgatgaatt gtcctggttc aatccttatt
    23161 tataataatg gtttacattc atataaagat ttgccaatcc ggatggctga acatggtcat
    23221 gtccaccgtc atgaaccaag cggttcactt aacggattat tcagagtgcg gagtttcact
    23281 caggatgatg cgcatattta ttgccgtaaa gatcaattac aaagtgaaat taaacgtttg
    23341 cttaaacttt atgacgaagt atataaaaca tttaatctta gttatcatat tgaactttca
    23401 acaagacctg aaaagaaata tattggtgac ttaaaaacat ggaatttaag cgaaaaagca
    23461 ctagcgaaag cgtgtattgc tagcggtatt ccttttaaaa ttaatgaagg agatggtgct
    23521 ttttacggcc caaaacttga ctttaaattg cgtgacagtt taggacgaat ttggcaatgc
    23581 ggaacgattc aattagatat gaatttaccc gaacgctttg atatttctta tatcgacgcg
    23641 aataaccaaa aagtacgccc aattatgctc caccgcgctg tctttggttc gattgaacgc
    23701 tttattggta tcttaattga acattacgct ggggccttcc cgctctggct atcaccaaca
    23761 caaattgctg tgttaccagt taataacgat gcccatttaa aacacgctaa aagtgttgtg
    23821 cgcaaacttg aaaaacaagg actccgtgtt gaacttgacg acagtaatga aaagttaggt
    23881 taccgtttac gtaatagtca aattaataaa attccttata cgcttgtcat tggtgacaaa
    23941 gagcgaaatg ataacttagt tacttatcgt aaatttggtg ataaagcaca aattactgta
    24001 ccttatcgtg aatttatcac ccttatcaag cgtgaaattc gtgacaaagc ataacgcaac
    24061 acaaactcct tcttctgtga gataataata gagaaaagga ggaaaaatta tggcatttac
    24121 attaaaaccg cttccatatg cttatgatgc acttgaacct tatatcgatg caaaaactat
    24181 ggaagttcac cattcaaaac accacgctac atatgttgct aatttaaatg cagcactagc
    24241 gaaagatcca tcgtttaacg attggagttt agaaaaaata cttaaaaact tagataaatt
    24301 accagaaagc attagaaccg cagttagaaa taacggaggc ggtgtctata accatgacct
    24361 cttctggact ttactcaaag ttaataacgg caaaggacca gaaggcaaac ttaaagatgc
    24421 actagtggca acctttggca cttttgaagc ttttaaagat gcttttgcta ccgcagcaaa
    24481 atcacgcttt ggctctggct gggcgtggct agtcgttact agtgacaaaa aactcaaggt
    24541 cgttagcacc ccaaaccaag atattccctt tgatgaagga accccaatct taacacttga
    24601 tgtgtgggaa cacgcttatt accttaaata tcaaaaccgt cgtccagaat atgttgaagc
    24661 tttctttaac gtcattaatt gggacgaagt cgcgcgttta tacgaaaatg cattaaaata
    24721 acaattaaat ttgacagatt atcatattaa tgatatcttt atatcgtcta aaagtggaaa
    24781 gtagaagcac ccgcttctca ccagatttac taggtaaatt ggttgatgct acgaattaat
    24841 cgttttatcg cgggtgtcta ctgacgcccg cttttattaa gtacaaggag gaaatgctta
    24901 tttacaacca aaacagagaa cggcccgaac gcaataccga tttagttaat gaaaacattc
    24961 ggtttcctga agtcttagtc attggtccta acggcgaaca actaggcgtc atgaaaacac
    25021 gcgctgctat tgacctagcg gcatctgaat acgaactaga tctctactgt gtgaacccga
    25081 acggcaaacc accagtttgc aaaatgctta attattctaa acaccgcttt gaacaacaaa
    25141 agcgcgctaa agaacaaaaa cggaaacaaa agactgtcga acttaaagaa attcgtatgt
    25201 cacccgtaat tgatgtacac gatcaagaaa caaaagggcg gatggctcgt cgtttcttag
    25261 aagatggtaa taaagtaaaa gttacaatcc gttatcgtgg acgccaaatg gcccacattg
    25321 atgttggtga aaaaatatta gaaagctttg ttgcatcact agaggattat gctattgtcg
    25381 aacgaaaacc gttaatggaa ggtcgtaact taagcgtcat cttagcgagt aaaattaaga
    25441 aataggagga agtttcatgc caaaaatgaa aagtaaaagt gctttaacca aacggattaa
    25501 agtgactaaa tcaggcaaat taatgcgtaa agctgcatat acatcgcatt tagccccccg
    25561 caaaacaaca aaacaaaaaa gacaattacg aaaagcgcgt ttagtgcata agagtgatgc
    25621 taaacgtatt aagtatttaa ttcagaatta ggaggaagga atcatggcaa gagtaaaaag
    25681 ttcaccggtc acacgtgccc gtagaaaaaa agttttaaaa caagctaaag gttactttgg
    25741 cgccaaaagc ttactcttta gaactgctaa agagcaagtt atgcactcct tacgttatgc
    25801 ttatatcgat cgtcgtcgga ttaaacgtga ttatcgtaaa ttatggatta aacgcattaa
    25861 cgccggctgt cggatgtgtg aaatcagtta tagtcaattt atgcacggtt taaaacttgc
    25921 aaatgttgat atcaatcgta aaatgttaag tgaattagca attaacgact ttgaaacatt
    25981 caaaggttta actgaaacag ctaaaaaagc actcgcaaaa taatataaat atttctttct
    26041 taataaaaat gggagtagat ttatgtttac tccctttttt aatgtttcac ttttcaataa
    26101 ttatagagtg tcacgaagag taggtaaatc tatactcata aagtcatata acttatatgt
    26161 attaagtgtg ccaaatttac taactagatt tttagcatat tctttaacag cggcaagagt
    26221 atcgcctatt ccccctttaa agtataaaat ttcattaggt atgggttcta atatcttagc
    26281 aatcgcttta actgtatgca cactaaaggc tattgcttct ttatgttcat gaaaagcttg
    26341 ttcatcatgg atacctagtt ttaaaaagcc ttcataacta ctttttattt cttgtttcaa
    26401 gtttttcatt atttcacttt gattcattta aatggccctc ttaattagtg ttcctttttc
    26461 tataaagtaa gctttaaacg gattatcttc aatttcaaga gcagaaatta gcgtcatatt
    26521 ctttatttcg caaacacttc taatcacgag ttcgaaatat cgccaattat aaattgggag
    26581 gtgagatctg cgtttaatga tgatgtaata atgctcttct tctttaaagg ctaaagcgtc
    26641 acccatatcg aacactctta agatatttgg taatttagta gttaatcttc ccatattatc
    26701 tttcttttaa aataaatctt cctttccatc atctaatcta atgtcaactt ttttcgtatc
    26761 acgaattgct tcactgatga gagattcata atcaaactta ctttcaaaaa gcactgcatc
    26821 ttctagttta ggactagtta ctgagtttgt aactgggaat attgtacagc aatcttcata
    26881 aggtaaaatt gaaattggat aagtttcaat gtctttagca atttttataa tttcattttt
    26941 atcgagcgtc gctaatgggc gaataattgg ataattagtg acttcattaa taacgacgag
    27001 tgattcaagt gtttgacttg aaacttgacc aagtgaatca ccagtcacta gtactttata
    27061 accgtgacgc ttagcaatga cagtactaat gcgaagcatc gtgcgtcgca ttaaggtaat
    27121 agcgtaacca cttgtagcgt gttcataaat cgctaattgg agtttagtaa atggtacgac
    27181 atataatcta atatgcggtt gatagttatt aagttttgcg gttagtgctt caattttctt
    27241 taatacttct tcacttgtat aaggtggagc cgcaaagtgg attgctgcaa ttttaattcc
    27301 gcgtctaatc attaaataag ccgcaactgg tgaatcaatt ccgccactaa ggagcactaa
    27361 accttttcca gcagcaccaa gcggataccc gcctaagccg tcataacgtt taccgacaac
    27421 aagaatacga cttttatcaa tttcaagacg aagtggtaat tcgggattat ggacatcaac
    27481 tttgatttca gcatttttta aaatatgact cgcgacactg cgattaattt cgtcgctcgt
    27541 taatggaaaa agtttatctt tacgtttagt aataacttta aatgtttttg gctttagttc
    27601 aaggatgagt tcaagcgcaa attgtttaac tttttctagg tctaacggga attctattac
    27661 ttcactaatt gcatggattc cgctgacatt ttgcaggcgt tttgtaattt cagcaacatc
    27721 tttttctttg atgtttttaa tataaatatg gtcatgcctt gtttcaattt gtactttgaa
    27781 attagcgaca acttctttaa tgttattagt caattgacga ataaaatcac gacggttttt
    27841 gcctttcgta taaagttcac caaaatgaat cattaacatt ttactcacgg ccatactcct
    27901 ttataatttc ggttaatgtg ttacaaaata tctcaacttc atcaagcgta ttttccattc
    27961 caaaacttac tcggattgag ttactagcgc gttcttcatc ttgcgaaatg gcatatactg
    28021 acttactcat tgcatgtaat ttagcgctac aagagctagt cgtgccaaca taaatatcgc
    28081 gactttccaa ggcgttgagc acgattgaag ctttaattgt ctttagtgaa aagttaacaa
    28141 tatacggatt acttccgccg accgaattaa taataaataa atctttatga gtagcaagaa
    28201 aattatgtaa tttaagtgct aagcgataag aatgatcata aaatttatcc atattacctt
    28261 cagcgatacg cactgttttt gctaatacaa tattcgccgg agcattatgt gttcccggcc
    28321 ttatcccttc ttcttgactg ccgccataca ctagtggctt tggctttaag tttttccgta
    28381 aaagtagtgc accacttcct tttaatccgc cgattttatg agcggaaaat gaataagcat
    28441 ctgcagcgtt gggatgaaag tcaagtttgc ctaaggcttg agtgacatca gtgtgaaata
    28501 taactttagg atattttttt agacatgctc taatctcccc taaatcaatg acatcaccaa
    28561 cctcattatt aacagccata acggagacaa gcaccgtttt atcatttaat agcgatttaa
    28621 aaacgtcaaa atcaaaacca cctttaaagc gaatcggcac tttatgaatg attgccccac
    28681 gttctttctc taaatattca catgcggcac gcacactagg gtgttcgtaa tcactaataa
    28741 ttatttcgtt accacgattt ttataagctt catataaacc aataagcgca atattatttg
    28801 cttcagtagc gccactagtg aaaataactt taccatcacg aaacttaaac attcttaaaa
    28861 tttcttcgcg tgatttatct tgtaatcttg ctatttcacg cgcaaaagtg tgcatcccac
    28921 ttggattgcc gtaatatttt tcaagaatct caatgtatgt tcttaaaact tcaggatgaa
    28981 tttttgttgt actagcgtta tcaaaataaa tcattgttta cggttttggt atttctttaa
    29041 ttgttcgatt gaattttcgt aagtatgttt aaaaagccca ttaaagaagc gttgttcttc
    29101 atcacggagt tttgaatgta attcactaaa ttgtggacgg aaagcgttaa gactaacgat
    29161 aacttcttcc gctgaatgag cgtactttac atcagttgta atcacatctt ctaagcgtgt
    29221 agcaagcgca cgtaattcat ttgttaaaac ttcgacttgg ctcacatcaa ttggtcgttc
    29281 acgaataatc gtgctgatat cactaatcat tgcgtaacca ctatcaaaat cttgttcaaa
    29341 gttttgaata tacgctggga cattaagctt acgcaaagta acttctaatt ctttaaagcg
    29401 aataaataaa agtttacaaa tcgcaaagcc atcttcaaca attacacgga gtgattcaat
    29461 atatgtttga aattctctaa cgagcacttc aactttcata ctctcagctt gcagttgttg
    29521 gactttctca actagcacac tatagggttg tttggttgca ctatggagaa aagcatctaa
    29581 gctacgctta accatcatca ttgtattaac attagtgtta gcttcttcta atttcttttc
    29641 atattcagga ttaattaaat aatactcttt cacttgcggc attcgattaa caagccgaat
    29701 atagcgttgt tctaattcat taacatgtaa atatgtttta tcgtattctg ccgtaaattc
    29761 gcgttgtttt tcaatttcaa cattgaaact atttattaat tcatcaattg tatcggcaat
    29821 ttgattaagt gtttgttcga cattgcgtgt atcaaaacga ataagttgtt ctttaatcac
    29881 ttcgagctgt tcattaaagc tttcaatttc agcactgaca cgtaaatggt gaagtggaac
    29941 attataagcc gttgcttctt cataactctt ctttaatgtc gcaatttttt cagggattaa
    30001 cactgtggcc ttcacaacta aaagcggtaa aatttgtaag atgcgttcaa gttgatcatt
    30061 aaatgtccga atcttttcta aataactacg agcttcgtca taataagcac cattaagagc
    30121 attatcatat tctttaaaaa gtaattcaat ctgtgtaaat attttttcaa agcttggtac
    30181 acaaatttcc agttcatctt tatgatcacg ataaatattt ttaataatcc gtaatttctc
    30241 tttatcgtaa acactctctt ccctaatttc tgattcgggt ttaatcatat tcgttaaatc
    30301 tttacttaac gctgtgactt tacgttcata atcaagaatg atggggcgat gttcattaaa
    30361 agctttttta aattctttat tacctgccgc gagtgcttca tttaatattt gaatactacg
    30421 acttgcttta cggtcatcga cattgcgtaa ttgttcaaag cgcgtaaaga attcttcata
    30481 agctggacgc attagtaaat taatatcact aattgcttcg atacgtttaa tatattgaat
    30541 attttcacca aggagtaggc tgcgcacata tgaatattta attgataaag attttaatgc
    30601 ctttttattt tttgtgccac ggaaggcaaa tttgcttaaa ataaatccga aaaccgctag
    30661 gacaagaatg atacttgcta caatgaagac aatatcgagt gtgtttaata atgttactac
    30721 tttcataagt cgcacctcac ttattgtata ttatacaatg tttgaaaatg ttttcatacg
    30781 ttaacatata tttggataga tgaataatta aaattaacaa gattaagtgt tttaatgatt
    30841 tt
//
LOCUS       DAOSTA010000009        29918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_1703_length_29918_cov_9.299468, whole genome shotgun sequence.
ACCESSION   DAOSTA010000009 DAOSTA010000000
VERSION     DAOSTA010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 29918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29918
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1703_length_29918_cov_9.299468"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1353
                     /locus_tag="PLU08_01670"
     CDS             136..1353
                     /locus_tag="PLU08_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70451.1"
                     /translation="MIKHILDTTEKIIDGSLPYQQFSKDLKQDNSYSQSEKHQFRTLV
                     NALLNRYFRYLYSLTEGEETTLTQFEVLALSLFSFNREKKVVPLSYEEFLAALPTDIQ
                     VKFEQEYPDESFPIRIPKHFHSENPALSLAARFSVPLALTEQLIADIGRKNLLKFLTN
                     RYRETYGLINQNVISDEDFFANMPLFIRGLKPGEYEYKGKEYIKKTPAYNNNEIVIAS
                     QTLLDVVDIIESKKPKNLLFVQHEDTSLMLVLALKYPEIQIHFYKDDNRSKYIVRHLV
                     RKFSLENVTYVDKITQTYDFVLTTLQSSNINGDIRHRDFYFRLPDNLQFFAAKGREEL
                     EELKDYVNEDGHLMFITTTVLRFETHLQAVGFLRDYPEFELILEKQHFHFIPNNETLY
                     YALLKKGAHVEEA"
     gene            1337..2401
                     /gene="rlmN"
                     /locus_tag="PLU08_01675"
     CDS             1337..2401
                     /gene="rlmN"
                     /locus_tag="PLU08_01675"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOQ70452.1"
                     /translation="MSKKLNFYDYSLPKLQDFLLNEGFKKYSATQMFEWVYLKDELDF
                     NNMSNLALVTREFMINNFTFALPTVHELQESKDGTVKLLLKLGDGSLVETVLMRYRYG
                     NSVCVSTQVGCNMGCRFCASGLLKKKRDLTPGEMLGQVLMINEYLKGRGELKLTTHVV
                     LMGTGEPFDNFENILDFIDLLNNPKSLAIGARHITVSTVGLVPKIKEFADLKLQVKLA
                     ISLHAATDEKRNQIMPINNAYPLKEVLSAVKYYESKTNKRVTYEYIMLNDFNDTLEDA
                     RALVNLVKGTNGFVNLIAYNPIAEFDYEPSTRDHVYKFANYLKSMGVQTTVRKEFGSD
                     IDAACGQLRAKVEKKRGNNS"
     gene            2385..3137
                     /locus_tag="PLU08_01680"
     CDS             2385..3137
                     /locus_tag="PLU08_01680"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOQ70453.1"
                     /translation="MATTVKGTFAAKSDRGLVRLHNEDRAAVLLNQKGDLLFVVCDGL
                     GGHNKGDFASKLAIDMLMTAFANNRGFLTTNSIKRFFNKNIRAINKAIFEESILSPDM
                     NNMSTTLNAVVLTRSKLCNVNIGDSRFYLCTDDEMYLMSEDQTVVGYLVRTNQITPEE
                     ALVHPKRHILTNALGTMPTVTFDFRKFQYRGERILVCSDGLYNMLDSEDILGILRTTQ
                     TAEFKVDALVALANHRGGADNIGVALWEPDND"
     gene            3130..4017
                     /locus_tag="PLU08_01685"
     CDS             3130..4017
                     /locus_tag="PLU08_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOQ70454.1"
                     /translation="MIRIGDLIDNRYRIVSLLGEGGMGTVYEAVDIITKRNVALKFLK
                     ANVARDKVNLARFEIEAKATATLNHPNIVRVISVGKYENLPYMVNELIVGNNLEDELQ
                     TRGRYTFLEAADIIDQLCNAVSLAHKNGVIHRDIKPSNIYITKEGLVKLGDFGIARYS
                     DSKRVTSNTNIVGSAHYLAPEVCRGGEASPRSDIYALGINFFELITGHVPFDGDSNVA
                     IAVKQIKEKFPSPRKYVKNTPKAIEKIIKKCCKKNPRERYPDVESLQQDILQLKTNPK
                     LLRPRQSIWVRWFGFVSED"
     gene            4018..4878
                     /gene="rsgA"
                     /locus_tag="PLU08_01690"
     CDS             4018..4878
                     /gene="rsgA"
                     /locus_tag="PLU08_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOQ70455.1"
                     /translation="MAGLVINKQKSSFIVEKNGETYVTKPSGNLIKSRQTIYVGDYVE
                     IDTENNLISELLPRRNFLVRPPIANVDIVLIVMSMVQPDFSLFLVEKFLSYANFAEIK
                     PLVIITKQDKLDTDDLLKEVQDNLDKLHVKNYVVNNKSGEGLDAVRKYLHGKVVALMG
                     QSGVGKSSFINALTDYDREVGKFSISINRGRHVTKEVIMLPFNAGYLVDTPGFSSFEL
                     PLTKGDLAENFPGFKKYIGTCKFNNCLHLSEIGCTVKADVEAGLISEESYNNYVMISD
                     ELKLYSEDYK"
     gene            4875..5540
                     /gene="rpe"
                     /locus_tag="PLU08_01695"
     CDS             4875..5540
                     /gene="rpe"
                     /locus_tag="PLU08_01695"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOQ70456.1"
                     /translation="MKQKVYVAPSLLAADFKNLHRELLILQQNKVKYLHFDVMDGHFV
                     PNLSFGIPILEALKPHYKFIYDVHLMVTNPEFIAPKFVEAGADIVTFHLEAMKTGNDV
                     VTLIKELRLMGVKVGISIKPDTPVEDLAPILKDIDLVLVMSVEPGFGGQSFLRSALHK
                     IEWLDEERKKYKYNYLIEVDGGINAETGALCAEAGADILVAGSYLFGKEDINERIKGL
                     RNG"
     gene            5533..6186
                     /locus_tag="PLU08_01700"
     CDS             5533..6186
                     /locus_tag="PLU08_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70457.1"
                     /translation="MDKSIVHIAFFSSLSLFVITLIFQLSLYRTKQNRKFSFRNELPF
                     ELVQGADIKFINYHYVLLFLVTIANLLFAFKYLDHIYNWYEYLLVGSLVLSAIMLYLI
                     FFIKVFEIKKHIIVVILQALSVVTSYLSFGLFAHISPFGKQNIVFGIFGYLFALIGML
                     VLLNPRLRKWPIMDKVLQQDGTVLILRPRYFMLALYEWGFIAAQFLLMIVMYAYLYV"
     gene            complement(6236..6427)
                     /gene="rpmB"
                     /locus_tag="PLU08_01705"
     CDS             complement(6236..6427)
                     /gene="rpmB"
                     /locus_tag="PLU08_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOQ70458.1"
                     /translation="MARHCPVTGKGPLSGNKRSHSLRATRRRWGVNLQNYKVEVDGKV
                     QTVRMSARAYRSLNKSAKV"
     gene            6594..7655
                     /locus_tag="PLU08_01710"
     CDS             6594..7655
                     /locus_tag="PLU08_01710"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HOQ70459.1"
                     /translation="MDLINKNIGEKIKSRRLRLGYSQLMLSELAHVSRDQISRIESGK
                     INFTLATLFKICRALRIKPHKLLKDAEQEEYIKSGDLRPFVKWAGGKTQILDELMQRI
                     PNEFNNYFEPFVGGGAFFLKLEHPRSVINDINSELMTAYSVIKDKEKFIALKKLLLQY
                     EEKHNEEFYYEVRARDRDPSFSTLSDVEIAARFIYLNKNCFNGLYRVNSKGYFNVPFN
                     KSEKVKTFDEDNINKLHKYLSLPSIKICCEDYKNMTNKAKKGDFVYFDPPYDTLKDDT
                     FTAYDKEGFGRNEQIELSEEFKKLHKKGVKVMLSNHNTPLINELYKEFNITIIHARRA
                     INSRGSGRGKIEEVIITNY"
     gene            7657..8448
                     /locus_tag="PLU08_01715"
     CDS             7657..8448
                     /locus_tag="PLU08_01715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOQ70460.1"
                     /translation="MNIINVGKSALKINDDDFILVQGNALRVLKKIAPKSVDVIFADP
                     PYFLSNDGITCVNGKMVSVNKALWDKSISATDKLNFNRKWIRYCRDALKDDGTIWISG
                     TMHNIYSIGVALELEGFSIINNITWRKPNPPPNIACRAFTHSTETVLWARKIINNRKK
                     GKHFFNYALMKEINGGKQMKDVWELKGALKKEKEHGKHPTQKPFELLERIILASSNEG
                     DIILDPFAGSGTTGIAAKRLKRRFIGIEIEKEYIGIIKKRYKAIQ"
     gene            8480..9340
                     /locus_tag="PLU08_01720"
     CDS             8480..9340
                     /locus_tag="PLU08_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HOQ70461.1"
                     /translation="MSRNFEEWLSTFRETIATWDYYTDFNKVYRNVDAVKVELNILNS
                     LIGSDDIRNEFISLVESYPHVLQVVPLLLAKRENTIKINTSETMYDFDFKNTNYSIEE
                     YANFMDKTGLFNLLKNRILRSLLDYVTGIEVGMDTNARKNRMGKAMEDLVEEHLKKKG
                     FVRDVTYFKEMSRSEIQSKFGLDLSNITNEGKAEKRFDFVIKTKDHVYAIETNFYSSS
                     GSKLNETARSYELLARESATVQGFTFIWITDGLGWRSARNNLRQTFEVTRHIYNIADL
                     ENDVLAVVLK"
     gene            9408..10103
                     /locus_tag="PLU08_01725"
     CDS             9408..10103
                     /locus_tag="PLU08_01725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOQ70462.1"
                     /translation="MKTLKANSVDLIFADPPYFLSNDGLSIRSGKVVSVNKGEWDKRD
                     NYDSTYMFTYSWLKECHRILKPTSSIWVSGTHHNIFEIKKVMDDLGFRIINLVIWQKT
                     DPPPLIYKNKLKFSHEILIWAKKGRSHFFNYDLAYSLRNSELGDVWKFSAVQNHEKKF
                     GYHPTQKPERLLERIIRLTTNKNDVVLDPFMGSGTTCFVAKKLQRQYIGIELEEEYFS
                     IAKRRIDSIKANT"
     gene            complement(10093..10542)
                     /locus_tag="PLU08_01730"
     CDS             complement(10093..10542)
                     /locus_tag="PLU08_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70463.1"
                     /translation="MKDKRIYTSFRGLLLIYLPIYLLMSIIFYLIVGIKFPPTLNHYL
                     TVGGWTIITALYLIFGYKNSYYELTKHEIVHHKGSNILYYKFDDIIYIDKVYSGKKLS
                     IRFFTRIGDERYLAHDPKKKVYNTMLERCTNLIDKEELLRRFPKIKY"
     gene            complement(10539..11606)
                     /locus_tag="PLU08_01735"
     CDS             complement(10539..11606)
                     /locus_tag="PLU08_01735"
                     /inference="COORDINATES: protein motif:HMM:NF039499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HOQ70464.1"
                     /translation="MANYELSRNTVSDLLSDNIQISPNTNEKLDYFRKAIKNTYPDYQ
                     KKFRHRARSFAVFAEIIIKRHNHTIKNNSIEHQKTYFKNDAYIYHIIEDFILAEEAKQ
                     NPEHTFTRDEYVDPTILQFENLIDHRYQNLLRYDFQKIKDPKLTLYNLTSRFFQELVS
                     GIMLLEREFYNDSFIIWRSLLETTTTLLILYENDNLVGKFNERRNIALMRVKVVDASR
                     QTLKSKAKETKQQLGFKGVPDYVAERYGWAGDLFKSRDYSLRTLLERINMVDLYSHYA
                     FASLFVHEYLISPEDLRLEIDFEKYLLSLYFKLYEAVRIKINDFTNDLDEVKKLEQGV
                     RKEVNNFKAQFNDFSTRIQTT"
     gene            11733..13148
                     /locus_tag="PLU08_01740"
     CDS             11733..13148
                     /locus_tag="PLU08_01740"
                     /inference="COORDINATES: protein motif:HMM:NF039513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HOQ70465.1"
                     /translation="MSLFTIIPSNFFSILASKNKEIYAEALLLLHHSIQDNELLIKKR
                     DFTRNLRDRLNELALSVDFDAEEEGDEDEIVNHSDKAAFIVRRLEECGWIDVEVNPDN
                     FEEYIALPSYSINVISLLNDLVKESTAEYVSLVHATYSELKLEDDMRDEFMYATLVRA
                     FENTKRLNVELITLAHSIRIFQNRLGKVFSTNNILSDYFDLYKTRVSDRYYHPLKTFD
                     SVAKFKRPILQILQKWLYDDDIRQQLVTQSLMWSRNKDAKAAEADIINKINYIADMYE
                     RVNETIDQIDQKHYEYTKASASKILYLNAADKSIKGHLDYIFKTYAETLSKGESVAPI
                     LNGINQSITFQQHGYITPDSLTLPIVRQLRAESIPMSLTDFDDVRDMLMQGFLDETSS
                     QFSNDAIFEFMDIAFDEESEVHISDIALPSFDAFLLLILATIKTSDEQCFYIIETEEG
                     SVSSYGYRLPNLTFVRKEITL"
     gene            13150..13788
                     /locus_tag="PLU08_01745"
     CDS             13150..13788
                     /locus_tag="PLU08_01745"
                     /inference="COORDINATES: protein motif:HMM:NF025205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HOQ70466.1"
                     /translation="MFEEAFLQLTKADQDAFAKAINELLLNSFIVRDEFDRREKTIKI
                     NPTYRFIERYYDLINAYLSFAGWRVDKDLILGVFSLVNEYNHNRLRFDREVSLVLFVL
                     RLIYEEERKEGGHVGDSVYLTTPLVMKVMHDRGIGFPNKRLSGRLIGRCLKEIAAHNI
                     IARVSGSYDEGNVTFYLLPSITFAVDNEKIVAISEALEKLALRDAGTEGGNV"
     gene            13785..17159
                     /locus_tag="PLU08_01750"
     CDS             13785..17159
                     /locus_tag="PLU08_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOQ70467.1"
                     /translation="MKLLKTVKIINWHYFWNQTLNFEQIVFLTGVNASGKSTLIDAMQ
                     VVLLGDTSGRHFNKAAAEKSNRTLRGYLRGELGDSLEGGFHYLRNGRFTSYIVLEFYD
                     DVNDDHFNLGIVFDVFDDGGVDHRFFMLEAPMPENEFIIDNVPMEYKDLNLFFQEHYP
                     NKYKFFDTNRQYQDALKRKLGNLKDKYFSLLKKAVSFSPITDITTFITEYVTEAQGNI
                     DIAPMQENILQYKKLEDEAALMRFKVERLNEIAKNYEVYKGHKEQLTLFEYVLEKATL
                     QNDLNRLATYDNQYLGYQKRLTDIESELADLDVDLAELNKRRVKLIQDSSSNDTYRIT
                     DEINEHKKVAEEKIKSLKFLNMQVRSDLENYVLTFDRLANEVLEKVSAMDEKRLKKDG
                     LYAREELLESAETIIKENHKITSVLNKDINELSETMLNDWRSAQAKFKQHLGAFNLSL
                     GRLINDYQVNEAILKQQELDLRGGGKPYEYSLVQIRNELQNKLSAKYGHKVEVSIFSD
                     LIDIRDAKWTNAIEGYLGGQRFHLFVEGEYFLDAYEFLKGLLQQYNFYGTNLVDVDAI
                     IERRFSAMPNSVAEEILTDHIGARAYADFLLGRLQKCNTIQEARNSGNGITPDTDLYR
                     NFTFGRINPRLYQQKYIGRRVEEADVNAKKAELAAHAETLRSYQELLQALLPGNDVEI
                     VNTNEIAALGSQVKRIQELPSLEQSIVFYDEELGRHDLTLIEGIHRRIGLVEADIKGI
                     EDQKSSLVEERGKISTNMQILKNERMPETKAIIRERQSKLEENYDAFFINDVAEPFFQ
                     NLIAEGKNPLDINAEYRQAFSNALYLSNSVFNQVKKLRKEYVVDYRLTYDVETLHNDD
                     FQMELNDLLEVKLPEYEEKIADAYRKAVKQFKDDFISKLRNAIETVEDQIAELNEALS
                     MSTFGEDSYQFTVKPSTVYRQYYDMIKDDLLLKVTDDDSEFFQKYDDVMKDLFAQIVL
                     AGDGDKNAAVLANVEKFTDYRNYLDFDLIVRNKDGHEQRLSRVLMKKSGGETQTPFYI
                     SVLASFAYLYRVNEPGELSNSARLIIFDEAFSKMDSGRIKESIRLLRRFGLQAIISAP
                     SDKVADISPLVDETLVVLRGKNHSTVQLFAREEDLT"
     gene            17179..18036
                     /locus_tag="PLU08_01755"
     CDS             17179..18036
                     /locus_tag="PLU08_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70468.1"
                     /translation="MEAKFVDGIRAYHMRLRRRYLVELFVTISVFLAFCIALIVLDLL
                     SDFNMREPLFVPLLASATSISLLLFILTYFPIRFKRLQKALNLEFFQRAKTVKLELIP
                     NPLGGTKFDYYTLFNEAGMNVKSGAIKGVAPYEFNKRLFHVVYVYDDEKYSAIIHLPS
                     IDSPYYIQIHNGNFAPPEAYADNLIVKIAYVSPYKLQYYASSSATNAKIYLRKENETR
                     FVKFIKIYQATYQYVTTFTDEYLKVEGLTSPGRLRLGEKYDNKHYHELLTRLRLLQAL
                     MSAMLERGK"
     gene            18041..19171
                     /locus_tag="PLU08_01760"
     CDS             18041..19171
                     /locus_tag="PLU08_01760"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015880913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOQ70469.1"
                     /translation="MAKELIAMILAGGKGTRLYELTRKDAKPAVHFGGKCRIIDYALS
                     NCAHSGIDTVGVLAQYESISLSRYIGNGEKWGLNGIRSSTVTIAPRQTDSGSNWFIGT
                     ADAIYQNLEFIDSYNPENVLILSGDHIYRMDYSKMLETHLENNADLTISVIEVTLEEA
                     KRFGILSADEDGRITEFVEKPPKPKSTLASMGIYIFNYKVLREALVKDAADENSSHDF
                     GKNIIPTLLANNNRLFVHRFDGYWKDVGTIDSLWEANMDLIEMMDEDFYQADKHYFRL
                     FSEDTNSVPQYIGKKAKVTNSIVNQGAIILGEVEGSIISNEVFIEVGAVVKDSVIMPG
                     VTVGRNAYVNKAIVANDLELPEHSKYNEAGEKVLLIHEGVVQ"
     gene            19168..20283
                     /gene="glgD"
                     /locus_tag="PLU08_01765"
     CDS             19168..20283
                     /gene="glgD"
                     /locus_tag="PLU08_01765"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HOQ70470.1"
                     /translation="MKVLAYLNLHHAPRLGPLTTRRSTAVVSFLGRYAIMDFMLSNFS
                     NSGFDRIATLVDNHPDSVLKHFGSRNTFNINTKLGLEIIAYNEGGSKAGPRYNTDIAN
                     IVFNDWILEQADPNYVVVAPSYFILPIDFRPILKQHIAKKSKVTAVYQRVNNARNEYI
                     GRAIFDLNKDGHVINISRNKGEKDARNIGLEIYIFNRETFDFLIRDAQNISSFYSLSD
                     IVAYHALKDIRVDTYEYVGYMRCIDSYQHYYDYSFELLNYRIRRQLFLHNWPIYTVTH
                     DTPPAKYEATADVKNAFIANGSLISGMIKNSIISRNVKIEEGAVVENSIIFTDSIVRR
                     NKVISHAIIDKKAEVAVAAELAGTPEDILYVRKGDRV"
     gene            20283..21716
                     /locus_tag="PLU08_01770"
     CDS             20283..21716
                     /locus_tag="PLU08_01770"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOQ70471.1"
                     /translation="MKILMVASESSPLAKTGGLADVVYALSKELAILGEEVSVVMPFY
                     GNIKRSPDQEIRFVANVQVPLGWRFQNAKVYKTFIDGITFYLLENEYYFGREGFYGYD
                     DDTERFAFFSVAVRNMMQALKIKPDVVHVHDYHGGMVPALIKIQNSRTKFFNKMKFVV
                     TIHNPAFQGEFHPDLLPDFYDIRKEYFDDGTLRYRDKVNALKAAIVFADKINTVSPTH
                     AEELLTPEGSKGLDGILKLRKNDFSGILNGIDYDEFNPQTDKHLPFRYGKTRPGIKTK
                     NKLVLFKEFGIKGDVDTPLFSMVSRLTWQKGIDLVLPAIVEVLEKGGNVVVVGSGEHG
                     YEQALEELRARYPDRMAIYIGYNNPLAHRVYAASDFFFMPSLFEPCGIGQMIAQRYGT
                     LPIVRTTGGLKDTVIGYDGTNEDVADGFVFDNYDEYWMKLTTNYALEQYKNQKVFKKL
                     RRNAMEKNNDWSEAGKKYLALYKSIVK"
     gene            21891..24290
                     /gene="leuS"
                     /locus_tag="PLU08_01775"
     CDS             21891..24290
                     /gene="leuS"
                     /locus_tag="PLU08_01775"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOQ70472.1"
                     /translation="MAYDFRAAEKKWQKYWEENNTFYTDMYDFSKPKYYVLDMFPYPS
                     GDGLHVGHVEGYTATDIVARFKRMQGYNVLHPIGFDAFGLPAEQYAIRTNNHPAIFTN
                     ENIANFRRQLKSLGFSYDFSKELSTSEPKYYKWTQWIFTELFKSDLAYVDNIPVNFCP
                     ELGTVLANEEVIDGKSEVGGFPVIRKPMRQWLLRITEYAERLLSDLDGLDWPASTLDM
                     QRNWIGKSEGTTITFKVANTNYEFDVFTTRVDTLYGATYACLAPEHPLVLKVVSDTQK
                     EEVKRYIESASHKSDLERTELNKDKSGVYLGVNAINPLNNKEIPLYVADYVLGSYGEG
                     AVMAVPAHDERDYEFAKRHGLPMVQVLAGDISENAFIDDAPHINSPLINGLNIEESKK
                     VLNEHLTNKGVGRVTTNYKLRDWLFSRQRYWGEPIPIMIKEDNTFIPVPLSDLPLTLP
                     PLKEYKPSGTGESPLALATSWVNTTYEGEKVKRETNTMPQWAGSCWYYIRYLDPHNDE
                     KIADPELIKHWLPVDLYIGGAEHAVLHLLYARFWHKFLYDKGVVNTKEPFTKLFHQGM
                     ILGENGEKMSKSRGNVVKPDDIIAEYSVDALRLYEMFMGPLEASLPWSNTGLEGALRF
                     LNRVARINSEDFPGKLVDTNNGELDYSYHYTVKKVTEDYEKLAFNTAIAQMMILVNDM
                     YKAREVYIPYYVGLIKLLYPIAPHLGEELYSQFVDVDTITYEAWPTYDEAKLVVNEVE
                     IAVQVNGKLRATIKAKLNESKEELEKAALTNENVQRFIDGLTIRRVIVVPNKIVNIVA
                     S"
     gene            24348..25130
                     /locus_tag="PLU08_01780"
     CDS             24348..25130
                     /locus_tag="PLU08_01780"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOQ70473.1"
                     /translation="MYLTVDIGNSVISIALFNAGEIVDVFNRETPKALKETAQANFDT
                     VRPIINEFKKQYAHSTFKKALISSVVPKLNYPMSFVIKEILGVDAEIVLPNPKTSVRL
                     DVDVPESVGSDIVACAVGAYKFGKGPFIVIDLGTANKYFYVDDKHVFHGVAISPGVEV
                     SFKGLVKNAAQLFNVRLTLPAKVLGKNTPDSLNSGLLYGLLGEMNGFINLIKKEVGDN
                     PIVLLTGGNARYIKGAVDESVNYVPHLVHYGLYDMLVNGNEK"
     gene            25120..27060
                     /locus_tag="PLU08_01785"
     CDS             25120..27060
                     /locus_tag="PLU08_01785"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HOQ70474.1"
                     /translation="MKNKKLVSDIAIDGLFIALILVLSLVPYLGFIQIGGISATILPI
                     PVILGAALLGPRRGVLYGAAFGFSSFLIAVIRGTAGDALFVDPLISIVPRILFGFCTA
                     IFSAVSFNERTSFKLKRFLIFPYSAIMMLLHSFFVLLAMYLRYVNAFMEYIFPILTPL
                     VLLEALVATVIVPVLYNVLYIPFEKYKDKFTTKNKSIYGTITSVYFADALNSLKEFVS
                     IKSVYDEKTVTKKTPYGKGVNEALEYMKNLATNDGFEAKIIDGRVVEIFVGEKYNKNI
                     AVFAHADVVPATGEWDTPPFTADIREGKLYGRGTSDDKGPAIAAYYAIKTLNNNNLLI
                     NYSVRLVIGGDEERGSSCMHYYFNEYNAPAPVHGFTPDAEFPLIYGEKGITNFTATKM
                     IDLGPISTITGGEAANSVIDKVVIRLLKDEDFIKYLTDNKVEHTVKMLSKNMDVTIFG
                     KSAHGSLPELGVNAGVLAFKHLGAFYKLPFLTHLAEKFKNPNGKTMDAYVATSLLGAT
                     TYNIGLLNYENGKLSFVVNFRYPENVEVETHLAKLAQTIDVELEIGRSSKHLLFDPKS
                     EFIQTLLKAYRDETGDTQSKPLAIGGGTYAKECPNTVAFGSAFPSRSGDIHSANEHIY
                     LDDFYTQMAIYARAIHYLGKKV"
     gene            27057..27677
                     /gene="trmB"
                     /locus_tag="PLU08_01790"
     CDS             27057..27677
                     /gene="trmB"
                     /locus_tag="PLU08_01790"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOQ70475.1"
                     /translation="MRVKFKPGAIDYLKSLAFHTHVDDFALSKIKDINKPLVLEIGVG
                     KGDYLLEMATNYPEHFFIGIELNASVLSVAAQKLADANLNNILILTGDVKYLLPKLSA
                     LSLNYIILNHSDPWPKKRHEKRRLTYSTLVNEYYKLLEEGGTLIFKTDNDDFADYSYI
                     SLSSVPFKELIFIKDYKGDYDFDAKTEYERKFLNKGVKIKMIIGKK"
     gene            27686..28756
                     /locus_tag="PLU08_01795"
     CDS             27686..28756
                     /locus_tag="PLU08_01795"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOQ70476.1"
                     /translation="MLTKRELKLLEKVTQIDAIPGLEQELAKFVKGELEKYGFTIVTD
                     NLGSVFGVKKSKVTQAPRIMVAGHLDEVGFSVREITDKGFIRVDARGGINANTMLASR
                     VRLTLDDGTKLMGSVGATAPHYGGEGNTTIDKLDFDFGFISKEDAVNAGVRVGQMIVA
                     DGPFEVLNGGKRLLAKAFDNRYGVFMALEMARYFENVDLPYDLYVGATVQEEVGLRGA
                     TTIANVINPDLVIILDCSPARDNISSSEQGQLGEGILIRYLDRSMIAFPELLTFQEKS
                     AEALKVPYQYFHSPGGTDAGAIHLHAVGVLTLTHCVCARSIHTSASVVDTTDIKNAKK
                     VLIRMLRTLNRDKINKFKEARR"
     gene            28761..29861
                     /locus_tag="PLU08_01800"
     CDS             28761..29861
                     /locus_tag="PLU08_01800"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOQ70477.1"
                     /translation="MTEIEMKNKDRKFRLTNKTFKFPSAIKDHFYTANYFVKTMEIAE
                     KHLADTVVTMQFFQRRDNVMACGVDESLALLQEFARYPEKLKIEACHDGDIVNANEPV
                     LKITGLYKDFAYLESLIDGILTRRTTAATNSYEVLQAAYPTEVFSMADRQDDYLTQAG
                     DGYASYIAGIKKFSTDAQGAWVNVKGMGTMPHALIAMTNGDLIKACELYHKTFPNEKI
                     SALVDYHNDTVTEALKVARHFGAKLGSIRLDNSKALLDEYFVRQGLNDPSLKGVSPEL
                     VFAVREALDKEGFNYVNIVVSSGFTPEKIKYFKAKNAPVNLYGVGSFIVNNKEVGFTG
                     DVVVVNGKPQAKVGRQEYFSDKLEKVTLKKRM"
BASE COUNT         9752 a         4698 c         5959 g         9509 t
ORIGIN      
        1 aaaatttaaa agtttcctaa tctttatttg taaagataag tatgtcatct aacagacgaa
       61 gacaaagacg actaattttc taattaatta gaaacacctt taaaaagagt gatatactaa
      121 tataggtagg tacatatgat aaaacacatt ttagacacaa cagaaaaaat cattgatggg
      181 agtctccctt atcaacaatt tagtaaggac ttaaagcaag ataatagtta ctcacaaagt
      241 gagaagcatc agtttcgtac tttagttaat gctcttttaa acagatactt ccgttatttg
      301 tattctttaa ctgaaggcga agaaacaact ctaactcaat ttgaagtttt agcattaagt
      361 ctctttagtt ttaatcgtga gaaaaaagtt gtgcctttat cttatgaaga gtttcttgct
      421 gcgcttccta ctgatataca agttaaattt gaacaagaat atcctgatga aagttttcca
      481 attcgaattc caaaacattt tcatagtgaa aacccagcgc tttctttagc cgctcgcttt
      541 agtgtaccat tagcacttac cgaacaatta attgcagata taggacggaa aaacctcctt
      601 aaattcctta caaatagata tcgtgaaaca tatggtctta ttaatcaaaa tgtaattagt
      661 gatgaagatt tctttgctaa tatgccactt tttattcgtg gcttaaaacc tggtgaatat
      721 gaatataaag ggaaagagta tatcaagaaa acaccagcgt acaataacaa tgaaattgta
      781 attgctagtc aaacattatt agatgttgtc gatattatcg aaagcaaaaa acctaaaaat
      841 ttgttatttg tgcaacacga agatacatcc ttaatgcttg tacttgcgct taaatatcct
      901 gaaattcaaa ttcattttta taaagatgat aatcgctcaa aatatatcgt gcgccattta
      961 gtacggaagt tttcattaga aaatgttact tatgtagata aaattaccca aacttatgac
     1021 tttgtgctca caacactgca aagttcaaat attaatggtg atattcgcca tcgtgatttt
     1081 tacttccggt taccagacaa cttacaattc tttgctgcaa agggccggga agagttagaa
     1141 gagctaaaag attatgtaaa tgaagatgga catttaatgt ttatcacgac taccgtattg
     1201 cggtttgaaa ctcacttgca agcagtgggt tttttgcgtg attatccaga gtttgaatta
     1261 atacttgaaa aacaacattt ccactttata cctaataatg aaacgctcta ttatgccctt
     1321 ttaaagaaag gtgcccatgt cgaagaagct taatttttat gactattctt tacctaaact
     1381 ccaagacttt ttattaaatg aggggtttaa aaaatatagt gcaactcaaa tgtttgagtg
     1441 ggtctattta aaagacgaat tagattttaa taatatgagt aatttagcgt tagttacacg
     1501 cgaatttatg attaataatt ttacttttgc attaccaaca gttcatgaac tacaagaaag
     1561 taaagacggt actgttaaac ttcttttaaa gcttggtgat ggatcactcg tcgaaacagt
     1621 tttgatgcgc tatcgttatg gcaatagcgt ctgtgtttca acgcaagtcg gatgcaatat
     1681 ggggtgtcgc ttttgtgcgt cagggctttt aaagaaaaag cgcgacttaa cacctggtga
     1741 aatgcttggt caagtattaa tgattaatga atatctaaaa ggtcgtggtg aattaaaact
     1801 tacaacgcat gtcgtactta tggggactgg ggagccgttt gataactttg aaaatatttt
     1861 agattttatt gacttattaa ataacccaaa atcacttgcg attggtgctc gtcatattac
     1921 agtttcaacg gtgggtttag tccctaaaat taaagaattc gccgatttaa aattacaagt
     1981 aaagttagcc atttctttac atgcggctac tgatgaaaag cggaatcaaa ttatgccaat
     2041 taacaatgct tatcctttaa aagaagtact ttctgccgtg aaatactatg aaagtaaaac
     2101 taataagcgc gtgacttatg aatatattat gcttaatgat tttaatgata ctttggaaga
     2161 cgcaagagcc ttagttaacc tagttaaagg cacaaatggt tttgttaatt taatcgcgta
     2221 taacccaatt gcggaatttg attatgaacc atcaactcgt gatcatgttt ataaatttgc
     2281 taattatcta aaaagcatgg gagtgcaaac aactgtacgt aaagagtttg gtagtgatat
     2341 agatgcggcc tgcggacaat tacgggcaaa agtggagaag aaacgtggca acaacagtta
     2401 aaggaacatt cgctgctaaa agtgatcgtg gcttagttag acttcacaat gaagatcgtg
     2461 ccgcagtcct tttaaaccaa aagggtgatt tactctttgt tgtttgtgat ggtcttggcg
     2521 gtcataataa aggtgacttt gcatccaaat tagcgattga catgctgatg actgcttttg
     2581 ctaataaccg tggtttttta acgacaaata gcatcaaacg tttctttaat aaaaatattc
     2641 gcgctattaa taaagccatc tttgaagaaa gtattttatc cccagatatg aacaatatgt
     2701 caacaacatt aaatgctgta gtattaacgc ggtcaaaatt gtgcaatgtt aatattggtg
     2761 atagtcgttt ttatttatgt acggatgatg aaatgtattt aatgagtgaa gaccaaacag
     2821 ttgtcggtta tttagtgcgc acaaatcaaa ttacccccga agaagcacta gtgcacccca
     2881 aacgtcacat cttaacaaat gcgcttggta cgatgccaac tgttactttt gattttagga
     2941 agtttcaata tcggggtgaa cgaattttag tgtgtagtga tggtctttac aacatgcttg
     3001 atagcgaaga tattttagga atattacgca caacacaaac agcggaattt aaagtagacg
     3061 cactcgtcgc tctcgccaat catcgcggtg gagccgacaa tataggagta gcattatggg
     3121 agccagataa tgattagaat aggtgattta attgacaatc gatatcggat tgtaagtttg
     3181 cttggtgaag gcggaatggg aacagtttat gaagcagtcg acatcattac caaacgtaat
     3241 gtcgcgctga agtttttaaa ggctaatgtc gctcgtgata aagtgaatct tgcccgtttt
     3301 gaaattgaag ccaaagcaac tgcgacttta aatcatccaa atattgttcg tgtcattagt
     3361 gtaggcaagt atgaaaattt accttacatg gtgaatgaat taatagtcgg taataactta
     3421 gaagatgaac tgcaaacgcg cggtcgctat acatttttag aagctgctga tattattgat
     3481 cagctgtgta acgctgttag tttagcgcat aaaaacgggg taattcatcg tgatataaaa
     3541 ccaagtaata tttatattac taaagaagga cttgttaaac ttggggactt tggtattgct
     3601 cgttatagtg actcaaagcg tgtcacaagt aacacaaata ttgttggtag cgcccattac
     3661 ttagcgcccg aagtgtgtcg tggtggcgaa gcttcaccac gcagtgatat ttatgcttta
     3721 ggtattaatt tctttgaatt aattacaggt catgttccct ttgacggtga ttcgaatgtg
     3781 gcaattgcgg taaaacaaat taaagagaag tttccaagtc cgcgtaagta tgttaaaaat
     3841 accccgaaag ctattgagaa aattattaaa aaatgttgta agaaaaatcc acgggaacgc
     3901 tatcctgatg ttgaatcatt acaacaagat atattacaac ttaagactaa cccaaaactt
     3961 ttacggccac ggcaatcaat ttgggtgcgc tggtttgggt ttgtctctga ggactagatg
     4021 gctggattag ttattaataa acaaaaatca agttttatcg tagagaaaaa tggtgaaact
     4081 tatgtaacta aaccaagcgg taatttaatt aaatcgcgcc agactattta tgtcggggat
     4141 tatgttgaaa ttgatacgga aaataattta attagtgagc ttctaccacg gcgaaacttt
     4201 ttagtaagac caccaattgc caatgttgat attgtgttaa ttgtcatgtc aatggtgcaa
     4261 cctgatttca gtctattttt agtcgaaaag tttcttagtt acgctaattt tgcggaaatt
     4321 aagccactag tcatcattac aaagcaagat aaacttgaca ctgatgactt gttaaaggaa
     4381 gtacaagata atcttgataa actacacgtt aaaaattatg ttgtcaataa taaaagtggt
     4441 gaaggacttg atgctgtgcg caaatatttg catggcaaag ttgtggcact tatggggcaa
     4501 tctggtgtcg gaaaatcaag ttttataaac gctttaacag attatgaccg cgaagttggt
     4561 aagttctcaa ttagcattaa ccgtggtcga catgtcacta aagaagtaat aatgcttccg
     4621 tttaacgccg gatatttagt tgatacacca ggcttttctt cctttgaatt accgctaacg
     4681 aaaggcgacc ttgccgaaaa cttcccggga tttaaaaagt atattggtac ttgtaaattt
     4741 aataattgtc tccatcttag tgaaataggc tgtaccgtta aggccgatgt tgaagctggg
     4801 cttatcagtg aagaaagtta taataattat gttatgataa gtgatgagtt aaagttatat
     4861 agtgaggatt ataaatgaaa cagaaagttt atgttgcccc atctttatta gctgcggatt
     4921 tcaaaaattt acatcgcgaa ctattaattt tgcagcaaaa caaggttaaa tatttacatt
     4981 ttgatgttat ggatggacat tttgttccaa atttatcatt tgggattcca attttagaag
     5041 cacttaaacc tcattataaa tttatttatg atgtacattt aatggttact aatcctgaat
     5101 ttattgctcc aaaatttgtg gaagctggag cggatatcgt tacttttcac cttgaagcaa
     5161 tgaaaactgg taatgatgtt gttacactaa ttaaagaatt acgactgatg ggtgtaaagg
     5221 taggtattag tattaaacct gatacacctg ttgaagactt agcgccgatt ttaaaagata
     5281 tcgatttagt gcttgtgatg tcagttgaac caggctttgg tggacaaagt ttcttacgta
     5341 gtgctttaca taaaattgaa tggcttgatg aagagcgcaa aaagtataaa tataattact
     5401 tgatcgaagt tgatggcggt atcaatgctg aaactggggc actttgtgct gaagcaggcg
     5461 ctgatattct tgtcgcgggt tcatacttat ttggtaaaga agacattaat gaaaggataa
     5521 agggattacg taatggataa gagtattgtt catattgctt ttttttcatc attatcactg
     5581 tttgtgatta ctttaatttt tcaattatca ctttatcgca ctaaacaaaa tcggaagttt
     5641 tcgtttcgta atgagctgcc gtttgaactt gtacaaggcg ccgatatcaa atttatcaat
     5701 tatcactatg tattattgtt tcttgtgacg atcgctaatt tattatttgc ctttaaatat
     5761 ttagatcata tttataattg gtacgaatat ttattagtcg gttcactcgt attaagtgcg
     5821 ataatgctct atcttatttt ctttattaaa gtatttgaaa ttaaaaaaca tattatcgtt
     5881 gttatcttac aagctttatc tgttgtcaca agttatcttt catttggttt atttgcgcac
     5941 attagtccat ttggtaaaca aaatatcgtc tttggtattt ttggttactt atttgcgctt
     6001 attgggatgt tagtgttact taatccacga cttcgcaaat ggccgattat ggataaagtt
     6061 ttacagcaag atggaacggt acttattttg cgaccgcgct attttatgct agcgctttat
     6121 gaatggggtt ttattgccgc acagttttta ttaatgattg taatgtacgc ttatctttat
     6181 gtttaagtag aaagaagaag ataataaaaa aaactcactt tgttgtgagc tttttttata
     6241 ctttggcgga cttgttaagt gagcgatatg ctcttgcaga catccggacg gtttggactt
     6301 tgccatcaac ttcaacttta tagttttgta agtttacgcc ccagcgccgt ctagttgcgc
     6361 gaagagagtg actgcgttta ttgccactta agggtccttt gcctgttact ggacaatgtc
     6421 tagccataat ttcctcccca ttcaataaat gctacattaa gataacatta atgttataaa
     6481 tttgcaaata taaaaagtta aattttgaaa attattgcaa atttgcacat attgttaata
     6541 aatacctttg ttattaatgt gtattttagt ccacattatg ttatactctc tctatggatt
     6601 taattaataa gaatatcggt gaaaaaataa aaagcagaag attgcgttta ggctactcac
     6661 aattaatgtt atcggaactt gctcatgtat cccgtgacca aatttcacgg attgaaagcg
     6721 ggaagataaa ttttacatta gcgacactat ttaaaatttg tcgggcgctt agaattaaac
     6781 ctcataaatt gcttaaagat gctgaacaag aagaatatat taaaagtggg gaccttcgtc
     6841 cttttgttaa atgggcgggt ggtaaaacgc aaatattaga cgaacttatg caaagaattc
     6901 ctaatgagtt caataattac tttgaacctt ttgtgggggg tggcgcgttc tttttaaaac
     6961 tcgaacaccc gcgttccgtc attaacgata ttaacagtga actaatgact gcttatagtg
     7021 ttattaaaga taaagaaaaa tttattgcac tgaagaaact attactacaa tatgaagaaa
     7081 aacataatga ggaattttac tatgaggttc gcgcgagaga ccgtgaccct tcattttcga
     7141 ctttaagtga tgttgaaatt gccgctaggt ttatatactt aaataagaat tgttttaatg
     7201 gtttgtaccg agtgaattcc aaaggttatt ttaatgtgcc atttaataaa agtgaaaaag
     7261 taaaaacttt cgatgaagat aatataaata aactacataa atatctgtca ttacctagta
     7321 ttaaaatatg ttgtgaagat tacaagaata tgactaacaa agctaaaaaa ggtgattttg
     7381 tttactttga tccaccttat gacacgctta aggatgatac attcactgcc tatgataaag
     7441 aaggctttgg tcgtaatgaa caaattgaac tttcggaaga atttaaaaaa ttacataaaa
     7501 aaggtgtgaa ggttatgctt tcaaatcata acacaccact gattaatgaa ctttataagg
     7561 agtttaatat tacgattatt catgctcgtc gcgctattaa ttcacgtggt agtgggcgcg
     7621 gtaaaattga agaagtgatt attactaatt actaaaatga atatcattaa tgttggaaaa
     7681 agcgcactta agataaacga tgatgatttt attttagtgc aaggtaatgc tttgcgcgtt
     7741 ttaaagaaaa tagccccaaa atcagttgat gttatttttg ctgacccacc ttactttcta
     7801 tcaaatgacg gtattacatg tgtaaacggt aaaatggttt ctgttaataa ggctttgtgg
     7861 gataaaagta tcagcgcgac agataaatta aatttcaacc gtaaatggat tagatattgt
     7921 cgcgatgctt taaaggatga tggaacaatc tggattagtg gcacaatgca taatatttat
     7981 tcaatcgggg tagcacttga attagaagga ttttctatta ttaacaacat tacatggcgc
     8041 aaacctaatc caccaccgaa tattgcttgt cgcgctttta cccattcaac cgagaccgtg
     8101 ctttgggctc gtaaaatcat taataaccga aaaaagggca aacatttctt taattatgca
     8161 ctaatgaaag aaattaacgg cggtaagcaa atgaaggacg tgtgggaatt aaaaggtgcg
     8221 ctaaaaaaag aaaaagaaca cggtaagcat cctacgcaaa aaccgtttga actactagaa
     8281 cgaattattc ttgcatcaag taatgaaggt gatattatac ttgatccctt tgctggaagt
     8341 ggcactactg gtattgccgc aaaaagatta aaaagacgtt tcattgggat agaaattgaa
     8401 aaagaatata ttggaattat taaaaaacgt tataaagcaa tacaataagt ttgtattatt
     8461 aaaataggag gcaatgatta tgagtcgaaa ctttgaagag tggttaagca cttttagaga
     8521 aactattgct acatgggatt attatactga ttttaataag gtttatcgta atgtagatgc
     8581 agtcaaagtc gaattaaata ttcttaatag tcttattggc agtgatgata ttcgcaatga
     8641 atttattagt cttgttgaaa gttatccgca tgttttacaa gtagtgcctt tacttttagc
     8701 caaaagagaa aacacaatta agattaatac ttcggaaaca atgtatgact ttgattttaa
     8761 aaatacgaac tattccattg aagaatacgc aaactttatg gataaaaccg gattatttaa
     8821 cttgctgaaa aatcgtattc tgcgtagctt gctcgattat gttactggaa tagaagtcgg
     8881 catggatacg aacgcccgta agaaccgtat gggcaaagca atggaagact tagtggaaga
     8941 acatcttaaa aagaagggct ttgtgcgcga tgttacttat tttaaagaaa tgagtcgaag
     9001 tgaaatccaa agtaaatttg gattagattt atctaatatt actaacgagg gaaaagctga
     9061 aaagcgtttt gattttgtaa taaaaacaaa agaccatgtc tatgcgattg aaacgaattt
     9121 ttattcttct tcaggctcaa aacttaatga aaccgccaga agttatgaac ttttagcaag
     9181 agaaagtgca acagttcaag gttttacctt tatttggata acagacggct tagggtggcg
     9241 ttcggctaga aataatctcc gtcaaacatt tgaagttact cgtcatattt ataatattgc
     9301 cgatttagaa aatgatgttt tagcagttgt cctaaaataa atgagaaata ttagtaatta
     9361 catttgtaat tattagtatg ttgtatttag gtgattgcac gaaagtaatg aaaacactaa
     9421 aagcaaatag tgtagattta atttttgctg atcctcccta ctttctttcc aacgacggtt
     9481 taagtattag aagtggtaag gttgtgtcag taaataaagg tgaatgggac aaaagagata
     9541 attacgatag tacatacatg ttcacttata gttggttaaa agaatgtcac aggattttaa
     9601 aaccaacaag ttcaatctgg gtaagtggca ctcatcataa tatttttgaa attaaaaagg
     9661 tgatggatga tcttggtttt cggataatta atttagtaat ctggcaaaaa acagacccac
     9721 caccattaat ttataaaaac aaattaaagt tttcgcatga aatattaatt tgggctaaaa
     9781 aaggaaggtc acactttttt aattatgacc tagcctatag tttgcgtaat agtgaacttg
     9841 gcgatgtttg gaaattttcg gcagtacaaa atcatgagaa aaaatttggt tatcatccca
     9901 cacaaaagcc tgaaagatta ttagaaagaa tcattcgatt aaccacaaac aaaaatgatg
     9961 tggtactaga tccatttatg ggtagtggca ctacttgttt tgtcgctaaa aaacttcagc
    10021 gtcaatatat tggtattgaa ttagaagaag aatatttttc aattgcaaaa agaaggattg
    10081 atagtataaa agctaatact taattttggg gaatctgcgt aataattcct ctttatcgat
    10141 aagattagtg cagcgttcaa gcatcgtgtt atacactttt ttctttggat catgcgctaa
    10201 atagcgctca tcgccaattc gcgtaaagaa gcggatacta agtttcttcc cggaatacac
    10261 tttatcaata taaatgatgt catcaaattt ataataaagg atattgctac ctttatggtg
    10321 cactatttca tgttttgtta gttcataata actattttta taaccaaata ttaaatataa
    10381 cgcagtaata attgtccagc cacccactgt tagatagtga tttaaggttg gtggaaactt
    10441 aataccaact atgagataga agatgatact cattaataaa tatattggta aataaattaa
    10501 aagtaatcct ctaaaacttg tgtaaattcg tttgtctttc atgttgtttg aattcgcgta
    10561 ctaaagtcat taaattgtgc tttaaaatta ttaacttctt tccgcactcc ttgttctaat
    10621 tttttaactt catcaaggtc atttgtgaag tcatttattt taatccgcac ggcttcataa
    10681 agtttaaagt aaagtgataa taaatatttt tcaaaatcta tttcgagccg taaatcttct
    10741 ggacttatta aatattcgtg gacgaataac gaggcaaagg cataatgcga ataaaggtca
    10801 accatgttaa ttctttcaag tagtgtccgt aaggaatagt cgcgcgattt aaataaatca
    10861 cccgcccaac cgtaacgttc tgcaacataa tctggcacac ctttaaagcc tagttgttgt
    10921 ttagtttctt ttgctttgct ttttagtgtt tgccgactcg catctacgac cttaacacgc
    10981 atcagcgcaa tgttgcgtct ctcattaaat ttaccaacta gattatcgtt ttcgtaaagt
    11041 attaacaagg tcgttgttgt ttctaagaga ctgcgccaaa tgatgaagga gtcattataa
    11101 aattcgcgtt ctaagagcat tattcctgac actaattctt gaaagaaccg gcttgttaag
    11161 ttgtaaagag ttagttttgg atcttttatt ttttgaaaat catatcttaa taaattttga
    11221 taacgatgat caattaagtt ttcaaattgt aagattgtcg ggtcaacata ttcatcacgt
    11281 gtaaaagtgt gttcaggatt ttgtttagcc tcctccgcta ggataaaatc ttcaatgata
    11341 tggtaaatat aagcatcatt tttaaaatat gttttttgat gttcaatgct gttattttta
    11401 atcgtatgat tatgtctttt gataatgatt tccgcaaata ctgcaaatga acgagcacga
    11461 tgtctaaact ttttttggta atcaggataa gtatttttaa ttgctttacg gaagtaatca
    11521 agtttctcat tcgtgtttgg tgaaatttga atattatcac ttaaaaggtc actaacagta
    11581 ttgcgactaa gttcataatt tgccatatgt taccgccttt ctattattat tttaaacgaa
    11641 attaaatatt tctcttttat tatacaagtt taataccaat tcattttttt atatgttaac
    11701 ataaaacata taaccgaagg agattatttt ttttgagtct atttacaatc atacctagta
    11761 acttcttctc gattcttgcg agtaagaata aagagatata tgctgaagca cttcttttac
    11821 tacaccattc aattcaagac aatgaattat taataaagaa gcgcgatttt actcgtaatt
    11881 tgcgtgaccg tttaaatgaa ctagcgctta gtgttgattt tgacgcggaa gaagaaggcg
    11941 atgaagacga aatcgttaat catagcgata aagcggcatt tatcgttcgt cgtttagaag
    12001 aatgcggctg gattgatgtt gaagttaatc ctgataattt tgaagaatac attgcgcttc
    12061 caagttatag tattaatgtt atttccttat taaacgattt agtgaaagaa agcactgctg
    12121 aatacgtttc gcttgtgcac gcgacttata gcgaacttaa gttagaagat gatatgcgcg
    12181 atgaatttat gtatgcgaca ttagtgcggg catttgaaaa tacaaaacgc cttaatgtcg
    12241 aacttatcac cttagcgcac agcatccgca ttttccaaaa tcgcttagga aaagtattta
    12301 gcactaataa cattttaagt gattattttg acttatataa aacgcgcgtg agtgaccgtt
    12361 attatcaccc gcttaaaaca tttgatagtg tcgctaaatt taagcgacca attttacaaa
    12421 ttttacaaaa gtggttgtat gacgatgata tccgtcaaca actagtgaca cagtcattaa
    12481 tgtggagccg caacaaagac gcaaaagcgg ccgaagccga tattattaat aaaattaact
    12541 acattgctga tatgtatgaa agagttaatg agacaattga ccaaattgat caaaaacact
    12601 atgaatacac gaaagctagt gcttcaaaga ttttatattt aaatgctgcg gataaatcga
    12661 ttaaaggtca ccttgattat atttttaaga cttatgctga aacattaagt aaaggtgaaa
    12721 gtgtcgcgcc gattcttaac gggattaatc aatcaattac attccaacaa catggttaca
    12781 ttacccctga ttcattaacc ttaccaattg ttcgtcaatt acgcgctgaa agtattccga
    12841 tgtcacttac tgactttgat gatgtgcgtg atatgttaat gcaagggttc cttgatgaga
    12901 caagtagcca attttctaat gatgcaatct ttgaatttat ggatatcgcg tttgatgaag
    12961 aaagtgaagt acatattagt gatattgctt taccgtcatt tgacgcattc ttacttttaa
    13021 ttttagcaac aattaaaacg agtgatgaac aatgttttta cataatcgaa accgaggaag
    13081 gtagtgttag tagctatggt taccgattac cgaacttaac attcgtacga aaggagatta
    13141 ccttataata tgtttgaaga agccttttta caattaacaa aagcagatca agacgccttt
    13201 gctaaagcaa ttaatgaatt attacttaat agttttatcg tgcgtgatga atttgatcgt
    13261 cgcgaaaaaa caattaaaat taatccgact tatcggttta ttgaacgcta ttacgactta
    13321 attaatgcct atttaagttt tgcaggctgg cgtgttgata aagatttaat attaggtgta
    13381 tttagcctcg ttaatgaata taatcacaac cgcttacgct ttgatcgtga agtaagctta
    13441 gtgttatttg tcttgcgcct aatttatgaa gaagaacgga aagaaggggg acatgttggc
    13501 gattctgtgt acttaacaac accacttgtg atgaaagtaa tgcatgatcg tggcattggt
    13561 tttccaaata aacgcctaag tgggcgctta attggtcgtt gtttaaaaga aatcgcggca
    13621 cataatatta ttgctagagt ctcgggaagt tatgatgagg gtaatgttac tttctactta
    13681 ttaccatcga ttacttttgc cgttgataat gaaaaaattg tcgcaattag cgaagctctc
    13741 gaaaaattag ccttacgcga tgcaggcacc gagggaggta atgtatgaaa ttattaaaaa
    13801 cagtcaaaat tattaattgg cactattttt ggaatcaaac cttaaatttt gaacaaatcg
    13861 tcttcctgac tggggtgaac gctagcggga agtcaacact tattgacgca atgcaagttg
    13921 tgctccttgg tgatacttcc ggccgccact ttaataaagc cgccgcggaa aaatcaaacc
    13981 ggacattacg cggatattta cgcggggaac ttggcgactc gttagaaggc ggattccact
    14041 atttacggaa cggacgcttt acttcttaca ttgttttaga gttttatgat gatgttaatg
    14101 atgatcactt taacttaggg attgtctttg atgtgtttga tgacggcggg gttgatcatc
    14161 gcttctttat gttagaagca ccaatgccgg aaaacgaatt tatcatcgat aatgtcccga
    14221 tggaatataa agatttaaat ttatttttcc aagaacatta tccaaataaa tacaaattct
    14281 ttgacacgaa ccgtcaatat caggatgccc ttaaacggaa acttggtaat ttaaaagata
    14341 agtattttag tctcttaaag aaagctgtta gtttctcacc cattacggat attacgactt
    14401 ttattactga atatgtcact gaagcacaag gcaatattga tattgcacca atgcaagaaa
    14461 atattttgca atataaaaag cttgaagacg aagcagcgct gatgcgtttt aaagttgaac
    14521 gcttaaatga aatagctaaa aactatgaag tatacaaagg tcataaagaa caattaacac
    14581 tttttgaata tgtcttagaa aaagcaacac tacaaaatga cttaaatcga ctagcaactt
    14641 atgataatca atatctaggt tatcaaaaac gcttaacaga tattgaaagt gaacttgctg
    14701 atttagatgt tgaccttgct gaacttaata aacgccgtgt taaattaatt caagatagtt
    14761 caagtaatga tacttatcgc atcactgatg aaattaatga acataaaaaa gtcgcagaag
    14821 aaaaaattaa aagtcttaaa ttcttaaata tgcaagtccg tagtgactta gaaaattatg
    14881 tcctaacatt tgatagactc gctaatgaag ttttagaaaa agttagtgcg atggatgaaa
    14941 aacggctaaa gaaagacgga ttatatgcgc gcgaagaact acttgaaagc gccgaaacaa
    15001 ttattaaaga aaatcataaa atcactagtg ttttaaataa agacattaat gaacttagtg
    15061 aaacaatgct taatgattgg cgcagtgctc aagcgaaatt taagcaacat cttggggctt
    15121 ttaacttaag tcttggtcgt ttaattaatg actatcaagt aaatgaagca attttaaaac
    15181 aacaagaact agatttacgc ggtggtggta aaccatatga atatagtctt gtgcaaattc
    15241 gcaatgaatt acaaaataaa ctttcggcaa aatatggtca taaagtcgaa gtaagtattt
    15301 ttagtgactt aattgatatt cgcgatgcga agtggacaaa tgcgattgaa ggttacttag
    15361 gtggccaacg cttccatctt tttgtcgaag gtgaatattt ccttgatgct tatgaatttt
    15421 taaaaggact tttacaacaa tataatttct atggcacaaa tttagtggac gttgacgcga
    15481 taattgagcg ccgctttagt gcaatgccaa attctgttgc tgaagaaata ttaactgacc
    15541 acattggggc aagagcatac gctgatttct tgcttggtcg tttacaaaaa tgtaatacaa
    15601 ttcaagaagc acgcaatagt ggtaatggta ttacgcctga tactgatctt tatcggaact
    15661 ttacttttgg gcgtattaat ccgcgcttat accaacaaaa atatattgga cgccgcgtag
    15721 aagaggcgga tgttaacgcg aagaaagctg aacttgctgc tcacgctgaa acattacggt
    15781 cttatcaaga gttactgcaa gcactcttac caggcaatga tgtcgaaatt gttaacacta
    15841 acgaaatcgc cgcccttggc agtcaagtta agcgtattca agaattacca agtcttgaac
    15901 aaagcattgt cttttatgat gaagaacttg gccgtcatga cttaacacta attgaaggga
    15961 ttcatcgccg gattggctta gtcgaagcag atattaaagg tattgaagac caaaaatcaa
    16021 gtttagtgga agagcgcggt aagatttcca caaacatgca aattttaaag aatgaaagaa
    16081 tgccggaaac aaaagcaatc attagagaac ggcaaagtaa acttgaagaa aattatgatg
    16141 ctttctttat taacgatgtc gccgaaccat tcttccaaaa cttaattgcc gaaggtaaga
    16201 atccgcttga tattaatgct gaatatcggc aagcatttag caatgcgctc tacctctcaa
    16261 atagtgtctt caatcaagtg aagaaactgc gtaaagagta tgttgttgat tatcgcttaa
    16321 catatgacgt tgaaacttta cacaatgacg acttccaaat ggaattaaat gacttgttag
    16381 aagttaaatt accagagtac gaagaaaaaa ttgcggatgc atatcgtaaa gcggttaaac
    16441 aatttaaaga tgacttcatt tcgaaattgc ggaatgcaat tgaaacggtt gaagatcaaa
    16501 ttgctgaact taacgaagca cttagtatgt ctacatttgg tgaagactcg tatcaattca
    16561 cggttaaacc aagtacagta tatcggcaat attacgatat gattaaagac gatttattac
    16621 ttaaagtaac ggatgacgat agtgagttct tccaaaaata tgacgatgtg atgaaagatt
    16681 tattcgcgca aatcgtgtta gcgggagacg gtgataaaaa tgcggcagtg ctcgctaatg
    16741 ttgaaaaatt caccgattac cgtaattatt tagactttga cttaattgtc cgcaataaag
    16801 atggccacga acaacgctta tcacgcgtat taatgaagaa gtctggtggg gaaacacaaa
    16861 ccccgttcta cattagtgta cttgctagtt ttgcttatct ctatcgcgtt aatgaaccag
    16921 gtgaacttag caacagcgcc cgtcttatta tctttgacga agcgtttagt aaaatggata
    16981 gtgggcggat taaagaatca attcgcttat tacgccgctt tggcttacaa gcaattatta
    17041 gtgccccaag tgataaagtg gctgatatta gccccttagt cgatgaaaca cttgttgtat
    17101 tacgcggtaa aaatcatagc acagtgcaat tatttgcgag agaagaggac ttaacttaac
    17161 cgcaaagaaa ggagaattat ggaagcaaag tttgttgacg gaataagagc atatcacatg
    17221 cgcttacgtc gccgctactt agtggaacta tttgttacca ttagcgtttt tctcgccttt
    17281 tgcatcgcct taattgtcct tgatctcttg agtgacttta atatgcgtga accacttttt
    17341 gttccgctat tagcgagtgc taccagtatt tccttactct tatttattct tacttatttc
    17401 ccaattcgct ttaaaaggct gcaaaaagcg cttaatttag agtttttcca aagagcaaag
    17461 acagtaaaac ttgaacttat tcccaatccg ttaggcggca cgaagtttga ttattataca
    17521 ctttttaatg aagcgggcat gaatgttaaa agtggggcga ttaaaggtgt tgcgccttac
    17581 gaatttaata agcggctttt tcatgttgta tatgtgtatg acgatgaaaa atatagtgcg
    17641 attattcact tgccaagtat tgattcacct tattacatcc aaatccataa cggtaatttt
    17701 gctccacctg aagcttacgc tgataattta attgtaaaga ttgcgtatgt ttctccctac
    17761 aaactacaat actatgcctc aagtagtgcc acaaatgcta aaatatattt acgaaaagaa
    17821 aatgaaacgc gctttgttaa atttatcaaa atttatcagg cgacttatca atatgtgaca
    17881 accttcactg atgaatattt aaaagttgaa gggctaactt caccgggtcg attacggcta
    17941 ggtgaaaaat atgataataa acattaccat gagcttttaa cgagattacg cttgttacaa
    18001 gccttaatgt cagcgatgct tgaaaggggg aagtaaaagg atggcaaaag agcttatcgc
    18061 aatgatttta gcgggaggta aagggacacg tctctacgag ctaacccgca aagacgctaa
    18121 accagcggtg cattttggcg gaaaatgccg catcattgac tatgccttaa gtaactgtgc
    18181 gcatagtggc atcgatacag ttggtgtctt agcccaatat gaatcgatta gtcttagtcg
    18241 ttatataggt aacggcgaaa aatggggatt aaacgggatt agaagtagta cagtaacaat
    18301 tgcgccccgg caaactgata gcggcagtaa ttggtttatt ggtactgctg atgcgattta
    18361 tcaaaacttg gaatttattg attcgtataa tcccgaaaat gttttaattt taagtggtga
    18421 ccatatttat cggatggatt attcgaaaat gcttgaaaca catttagaaa ataacgctga
    18481 tttaacaatt tctgtcattg aagtcacact tgaagaagct aagcgctttg ggattcttag
    18541 cgctgatgaa gacggacgga ttactgaatt tgtggaaaaa ccaccaaaac caaaatcaac
    18601 actagcgagt atgggtattt atatctttaa ctataaagta ttacgcgaag cactcgttaa
    18661 agatgccgct gatgaaaata gttcccatga cttcggtaaa aatatcattc caactttatt
    18721 agcgaataat aatcgcttat ttgtccatcg ctttgatggg tattggaaag atgttggtac
    18781 gattgattca ttatgggaag caaatatgga tttaattgaa atgatggatg aagatttcta
    18841 tcaagccgat aaacattact tcaggctttt tagtgaagac acaaatagtg tgccgcaata
    18901 cattggtaaa aaggcgaaag tcacgaatag tattgtcaat caaggcgcca ttattttagg
    18961 tgaagttgaa ggatcaatta ttagtaatga agtttttatt gaagtagggg cagttgtaaa
    19021 agacagtgtc attatgcctg gcgttactgt cgggcgtaat gcttatgtta ataaagcaat
    19081 tgtcgctaat gatttagaac ttccagaaca ttctaaatat aacgaagcgg gtgagaaagt
    19141 attactcatc catgaaggag tagtgcaatg aaagttttag cttatcttaa tttacaccat
    19201 gccccacgcc ttggaccatt aacgacgcgg cgttcgactg ctgttgttag ttttttaggg
    19261 cgttatgcga ttatggactt tatgcttagt aactttagca atagcggatt tgaccggatt
    19321 gcaacactag tcgataatca tcctgattca gtattaaaac attttggtag tcgtaatact
    19381 tttaacatta acactaagtt agggttagag attattgctt acaacgaagg cggttcgaaa
    19441 gctggtccgc gttataacac ggatatcgct aatatcgtct ttaatgactg gattttagag
    19501 caagctgatc caaattatgt tgtcgttgct ccaagttact ttatattacc aattgatttt
    19561 aggccgattt taaagcaaca tattgctaaa aaatcaaaag taacagcggt atatcaacgc
    19621 gttaataatg cgcgaaatga atatattgga cgcgctatct ttgatttaaa taaggacggt
    19681 catgttatta atatttcacg aaataaaggt gaaaaagatg cacgcaatat cggacttgaa
    19741 atttatattt tcaatcgtga aacatttgat ttcctcattc gtgatgccca aaacattagc
    19801 tcattttatt ccttaagtga tattgttgct taccatgcct taaaagatat aagggtcgat
    19861 acttatgagt atgtcgggta tatgcgctgt attgatagtt atcagcatta ttatgattat
    19921 agttttgaat tacttaacta tcgtatccgt cgtcaactct tcttacataa ttggccgatt
    19981 tacaccgtaa cgcatgatac cccaccagcg aagtatgaag caacggctga tgtcaaaaat
    20041 gcgtttatcg ccaacggatc attaatcagc gggatgatta aaaattcgat tatttcacgg
    20101 aatgtcaaga ttgaagaagg ggcggttgtg gaaaattcca ttatctttac cgattcaatc
    20161 gttagacgca ataaagtgat tagtcatgct attattgata agaaggcgga agtggcggtg
    20221 gctgctgaac tagcgggtac gccggaagat atactatatg ttagaaaagg agatcgagtg
    20281 taatgaaaat attaatggta gcaagtgaaa gtagtccact tgcaaaaact ggtggcttag
    20341 ccgatgtcgt ctatgcctta agtaaggaac ttgcgattct tggggaagaa gtaagtgtag
    20401 tgatgccatt ttacggcaat attaaacgga gtcctgatca agaaatccgc tttgtcgcga
    20461 atgtccaagt ccctttaggc tggcgctttc aaaacgctaa agtgtataaa acatttattg
    20521 acggtattac attttattta ttagaaaatg aatactattt tggccgtgaa gggttttatg
    20581 ggtatgacga tgatacggaa cgctttgcgt tttttagtgt cgctgttcgc aatatgatgc
    20641 aagcattaaa aattaaacca gatgtcgttc atgtccacga ttatcatggc ggcatggtac
    20701 cagcgttaat caaaattcaa aattcacgca caaaattctt taataaaatg aaatttgttg
    20761 tcacaattca taatccagca ttccaaggtg aattccatcc ggatttactc cctgattttt
    20821 atgatattcg gaaggagtat tttgatgacg gaacattacg ttatcgtgac aaggttaatg
    20881 cgcttaaagc tgcgattgtc ttcgctgata aaattaatac cgttagtcct acgcacgcgg
    20941 aagagctttt aacccctgaa gggtcaaaag ggttagacgg tattttaaaa ttacggaaaa
    21001 atgacttcag cggtattctt aacggcatcg attacgatga atttaatcca cagactgata
    21061 aacatttacc gtttagatat ggtaaaacta gaccaggaat taagacgaaa aacaaacttg
    21121 tactatttaa agaatttggt attaaaggtg atgttgatac accgctattt tcgatggtta
    21181 gccgtttaac gtggcaaaaa ggcattgatt tagtgcttcc cgctattgtt gaagtattag
    21241 aaaaaggtgg caatgttgtc gttgtcggta gcggggaaca tgggtatgaa caagcgcttg
    21301 aagaactgcg cgctcgctat ccagaccgta tggcaattta tatcggttac aataatccac
    21361 ttgcgcaccg cgtttacgca gcgagtgatt tcttcttcat gccaagttta tttgagccgt
    21421 gcggtattgg gcaaatgatt gcccagcgct acgggacgct gccaattgtg cgtactaccg
    21481 gtggtttaaa agatacggta attgggtatg atggcactaa tgaagatgtc gctgatggtt
    21541 ttgtctttga taattatgac gaatactgga tgaaactcac gacgaattat gcgcttgaac
    21601 aatacaaaaa tcaaaaggtc tttaaaaagc ttcgccgcaa tgcgatggag aaaaataatg
    21661 actggtctga agctggtaaa aaatacctag cgctctataa aagtatcgtt aaataattat
    21721 tgtgatataa ttttatcgcg agtgaagaat aaataactta tattaagtta tgtctcacct
    21781 tcagttaact cgctgtgccc tagcattaat aggaaatgaa gttataaaat aaggtggtac
    21841 cgcggacttt ccgccctttg gtaacacctt ttatttttgg aggtaacatt atggcttatg
    21901 attttcgtgc cgcagaaaag aagtggcaaa aatattggga agaaaataac acattttata
    21961 cagatatgta tgactttagt aaaccaaagt attatgtgtt agatatgttt ccctatcctt
    22021 caggcgacgg actgcatgtt ggtcacgttg aagggtatac cgcgactgat atcgtcgctc
    22081 gctttaaacg gatgcaaggc tataatgtct tacacccgat tggatttgac gcctttggtt
    22141 taccagcgga acaatatgcg attcgcacga ataatcatcc ggctattttt actaacgaaa
    22201 atatcgctaa ttttagacgg caattaaagt cgcttggctt tagttatgat tttagtaaag
    22261 aactttctac tagtgaacca aagtattata aatggacaca gtggattttc actgaacttt
    22321 ttaaaagcga cttagcctat gtcgataata tccctgttaa cttttgtcct gaactaggaa
    22381 cagtactcgc aaatgaagaa gtaattgacg gtaaaagtga agttggcgga ttcccggtga
    22441 ttcgtaaacc gatgcgccag tggttattac gcattacgga gtacgcggag cggttattaa
    22501 gtgatttaga cggtttagat tggcccgctt ctacgcttga tatgcaacgt aactggattg
    22561 ggaagagcga aggcacaacg attactttta aagtagcgaa tacaaattac gagtttgatg
    22621 tctttacaac gcgtgttgat acgctttatg gggcgactta tgcatgttta gcgcctgaac
    22681 atccgcttgt gttaaaagtt gttagtgaca cgcaaaaaga agaagtaaag cgttatattg
    22741 aaagcgctag tcataagagc gacttagaac ggacagaact taataaagat aaatcagggg
    22801 tgtatttagg agttaacgct attaatccgc ttaataacaa agaaatcccg ctttatgttg
    22861 ctgattatgt tttagggtca tatggtgaag gcgcagtaat ggcggttcca gcgcatgacg
    22921 aacgtgatta cgaatttgct aagcgccatg gcttaccgat ggttcaagtg ttagcaggcg
    22981 acattagtga aaatgctttt attgatgacg ccccccatat taatagtccg cttattaacg
    23041 ggcttaatat tgaagagagt aaaaaagtac ttaatgaaca cttaacgaat aaaggagtag
    23101 gtagagtaac gactaattat aaattacggg actggttatt ctcacgtcaa cgctactggg
    23161 gtgaaccaat tccaattatg attaaagaag ataacacttt tatccctgta cctttaagtg
    23221 atttaccgct tactttaccg ccgttaaagg aatataaacc tagcggtact ggtgagtcgc
    23281 cacttgcgct tgcaactagt tgggttaata caacttatga gggtgaaaaa gttaaacgcg
    23341 aaacaaatac gatgccgcaa tgggcggggt catgctggta ttatattcgt tatttagatc
    23401 cgcataatga tgaaaaaatt gctgatccag aacttattaa gcactggctc cctgttgatc
    23461 tttatattgg tggggcggaa cacgcggtct tgcatttact ttatgcgcgt ttttggcata
    23521 agtttttata tgataaaggt gttgtaaata caaaagaacc atttactaaa ttattccatc
    23581 aagggatgat tttaggggaa aatggtgaaa aaatgtcgaa gtcacgtggt aatgtcgtta
    23641 aacctgatga tattattgct gaatatagtg ttgatgcttt gcggctttat gaaatgttta
    23701 tgggtccact tgaagcaagc ttaccgtggt caaatacagg gttagaaggt gcgctccgtt
    23761 tcttaaatcg tgtcgcacga attaatagtg aagatttccc tggaaaactt gtggatacga
    23821 ataatggtga gcttgattac tcgtatcact acactgttaa aaaagtgact gaagactacg
    23881 aaaaattagc gtttaatacc gcgattgcgc agatgatgat tttagttaat gatatgtata
    23941 aagcgcgtga agtttatatt ccttattatg tcggtttaat taaattactt tatccaatcg
    24001 ctccacattt aggcgaagaa ctttactcac aattcgttga tgttgacaca ataacttatg
    24061 aagcgtggcc aacttacgat gaagcaaagt tagtcgtaaa tgaagttgaa attgctgtgc
    24121 aagttaatgg taaattacgc gcgacaatta aagctaaact taatgaaagt aaagaagaac
    24181 ttgaaaaggc cgcgctcact aatgaaaatg tccagcgttt tattgatggt ttaacgatta
    24241 gacgggtcat tgtcgtccca aataaaatcg taaatatcgt tgcttcttag gaattatata
    24301 ttataatata ttacgcactt atttagtaaa agtggtgggg agagcttatg tatttaacag
    24361 ttgatatcgg aaatagtgta attagtattg cgctatttaa cgctggtgaa attgttgatg
    24421 tttttaatcg tgaaacgccg aaagcgctaa aagaaacggc acaagcaaac tttgataccg
    24481 ttcgtcccat cattaacgaa tttaaaaagc aatatgcgca cagtacattt aagaaagcgc
    24541 ttatttctag tgttgtcccg aagttaaatt atccaatgtc atttgtgatt aaagaaattt
    24601 taggagtcga tgctgagatt gttttaccaa atcctaaaac tagtgtgaga ctagatgttg
    24661 atgttcccga aagcgttggt agtgatattg tggcttgcgc tgttggtgct tataaatttg
    24721 ggaaaggtcc ttttattgtt attgatttag ggacggctaa taaatatttt tatgttgatg
    24781 ataaacatgt ttttcacgga gtggcaatta gtcctggtgt ggaagtatcg tttaaaggtt
    24841 tagtaaaaaa tgcagcacaa ttatttaatg tccgccttac tttacccgcg aaagtactcg
    24901 gtaaaaatac acccgactcg cttaatagcg gtttattata tggtttatta ggggagatga
    24961 acgggtttat taatttaata aaaaaagaag taggagataa tccaattgtg ttattaactg
    25021 gtggtaatgc acgctatatt aaaggtgctg tggatgaaag tgtaaattat gttccacatt
    25081 tagtgcatta cgggttatat gacatgttgg taaatggcaa tgaaaaataa aaagttagta
    25141 agtgatattg caattgatgg gttatttatt gcccttattt tagtgttaag tcttgtcccg
    25201 tatttaggct ttattcaaat cggggggatt tcggcgacaa tcttaccaat tcctgtaatt
    25261 ttaggagcgg cacttttagg acctagacgc ggtgtgcttt atggcgctgc ttttggtttt
    25321 tcgtcctttt taattgctgt tattcgtggt actgctgggg acgcattatt tgttgatcct
    25381 ttaattagta ttgtgccgcg gatattattt ggattttgta ccgcaatatt ttcagcggtt
    25441 tcatttaatg aacgcactag ttttaaatta aaacgtttcc tgatattccc ttattcagca
    25501 attatgatgc tgttacattc attttttgtc ttacttgcaa tgtatttgcg ttatgtaaat
    25561 gcttttatgg aatatatttt cccgattctt actccgcttg tattacttga agctttagtg
    25621 gcaactgtta ttgttcctgt tttatacaat gttttataca ttccttttga aaaatataag
    25681 gataaattca ctacgaaaaa taaatcaatt tatggaacga ttaccagtgt ttattttgcc
    25741 gatgctttaa attctttaaa agaatttgtg agtattaaat cggtatatga cgaaaaaaca
    25801 gttacgaaaa agacaccata tggtaaaggg gttaacgaag cattagagta tatgaaaaat
    25861 cttgccacaa atgacggatt tgaagcgaaa attattgatg gccgtgtcgt tgaaatattc
    25921 gttggtgaaa agtataacaa aaatattgct gtttttgctc acgctgatgt cgtgccagca
    25981 actggcgaat gggatacacc accatttacc gctgatattc gtgaaggaaa actctacggc
    26041 cgtggcacaa gtgatgacaa aggaccagcg attgctgctt attacgcaat taaaactttg
    26101 aataataata acttattaat taattatagt gtccgtcttg taattggtgg cgacgaagaa
    26161 cggggtagca gttgtatgca ctattacttt aatgaatata atgcgcctgc accggtacac
    26221 ggttttacac ctgatgcgga gtttccgcta atatatggtg aaaaaggtat tactaatttc
    26281 accgcgacaa agatgattga tttaggtcca ataagcacga ttactggtgg tgaagctgct
    26341 aatagtgtga ttgacaaagt cgtcattcgg cttttaaaag atgaagattt tattaaatat
    26401 ttaacggata ataaggttga gcatacagta aaaatgttat cgaaaaatat ggatgtaaca
    26461 atttttggta aaagtgcgca tggtagttta cctgaacttg gtgtgaacgc cggggtactt
    26521 gcttttaaac atttaggtgc tttctataaa cttccatttc ttacccattt agcggaaaaa
    26581 tttaaaaatc caaacggtaa aactatggat gcatatgtcg caacctcact gcttggtgct
    26641 acgacatata atatcggatt actaaattat gaaaacggga aattaagttt tgttgttaat
    26701 ttccgttatc cggaaaatgt ggaagtggaa acgcatttag caaaattagc gcaaacaatc
    26761 gatgtcgaat tagaaattgg tagaagtagt aaacatttat tatttgatcc aaagagtgaa
    26821 tttattcaaa cactcttaaa agcatatcgc gatgaaacag gcgacacgca aagtaagcca
    26881 cttgctatcg ggggcggaac ttatgctaaa gaatgtccta atactgttgc atttggtagt
    26941 gcttttccta gtagaagcgg ggatattcat tccgctaacg aacatattta tttagatgat
    27001 ttttatacac agatggcaat ttacgctcgt gctattcatt acttaggtaa gaaggtatga
    27061 gggtaaaatt taaaccaggt gctattgatt acttaaaatc attagcgttt cacactcacg
    27121 ttgatgattt cgcattgtca aaaattaaag atattaataa accgttagtg ctagaaattg
    27181 gtgttggtaa gggcgattat cttttagaaa tggctactaa ctatcccgaa cactttttta
    27241 ttggtattga attaaatgca agcgtcctta gtgttgccgc gcaaaaactc gcggacgcaa
    27301 acttaaataa tatcctaatc ttaacaggtg atgttaaata tttattacca aaattaagtg
    27361 cactttcact taattacatt attttaaatc atagtgatcc gtggccaaaa aagcgccatg
    27421 aaaagcgccg tttaacttac tcgacactcg ttaatgagta ttataagctt ttagaagaag
    27481 gtggcacact aatttttaaa acggataatg atgacttcgc ggattattct tacatctcgt
    27541 taagtagtgt tccctttaaa gagcttattt ttataaaaga ttataagggt gattatgatt
    27601 ttgatgctaa aacggaatac gaacgcaaat tccttaataa aggtgttaaa attaaaatga
    27661 ttattggaaa gaaataggtg tttatatgtt aacaaaacgt gaactcaaat tacttgaaaa
    27721 agtaacgcag atcgatgcaa ttcctggctt agaacaagaa ttagcaaagt ttgtcaaagg
    27781 cgaattagaa aaatacggtt ttacaatcgt tacggataat ctcgggtcgg tttttggtgt
    27841 aaagaaaagt aaagtaactc aggcaccgcg cattatggtt gctggtcatt tagatgaagt
    27901 tggttttagt gtccgtgaaa ttactgataa aggttttatt cgtgttgatg ctaggggcgg
    27961 tattaacgct aacacgatgt tagcgagtcg cgtacgtctt acgcttgatg atggcactaa
    28021 attaatggga agcgttggtg cgaccgcgcc gcattatggc ggagaaggta atacaacgat
    28081 tgataaactt gattttgact tcggctttat tagtaaggaa gacgcagtta atgctggtgt
    28141 tcgtgttgga cagatgattg ttgcagatgg tccttttgaa gtattaaatg gtggcaagcg
    28201 cttactcgct aaagcttttg ataaccgcta cggggtattt atggcgcttg aaatggcacg
    28261 gtactttgag aatgttgatt taccatatga cttatatgtc ggtgccaccg tgcaagaaga
    28321 agtaggatta cgcggggcaa caactattgc caatgtaatt aatccagatt tagtgatcat
    28381 tttagattgt tcgcccgcgc gcgataatat tagtagcagt gaacaaggac aactcggcga
    28441 aggaatttta attcgttatt tagatcgctc gatgattgct tttccggaat tattaacatt
    28501 ccaagaaaaa agtgctgaag cactaaaagt tccctatcaa tattttcata gtccaggtgg
    28561 gactgatgcg ggggccattc atttacacgc tgttggtgtt ttaacactga cgcactgtgt
    28621 ttgtgcgcgc tctattcaca cttccgctag tgttgttgat acgactgata ttaaaaatgc
    28681 taaaaaagta ttaattcgca tgttaagaac attaaatcgc gataagatta ataaatttaa
    28741 agaagctagg agataagaaa atgacagaaa ttgaaatgaa aaacaaagat cggaagtttc
    28801 gtttaacaaa caaaaccttt aaatttccaa gtgcgattaa agatcatttt tataccgcga
    28861 attactttgt aaaaacaatg gaaattgctg aaaaacattt agcggacaca gtcgtgacga
    28921 tgcaattttt tcaacggcgg gataatgtga tggcgtgtgg tgtagatgaa tcactagctt
    28981 tactacaaga atttgcgcgt tatcctgaaa aattaaagat tgaagcgtgt catgatggtg
    29041 atattgttaa cgcgaatgaa ccagtgttaa aaattacagg tttatacaaa gattttgcgt
    29101 atttagaaag tcttattgat ggcattttaa cgcggagaac gacagcagca accaatagtt
    29161 atgaagtgtt gcaagctgct tatccaactg aagtttttag tatggcagac cgccaagatg
    29221 attatttaac acaggcggga gatggctacg ctagttatat tgcggggatt aaaaagtttt
    29281 ccactgatgc gcaaggtgcg tgggtaaatg ttaaagggat gggaacaatg ccacatgctt
    29341 taattgcgat gactaatggt gacttaatta aggcttgtga actttatcac aaaactttcc
    29401 caaatgaaaa gatcagtgcg cttgttgact atcataatga tacagtgaca gaggccttaa
    29461 aagtagcgcg tcattttggt gcgaaattag gctcgattcg tcttgataat agtaaagcat
    29521 tacttgatga atattttgtg cgtcaaggtt taaatgatcc aagtctaaaa ggtgttagtc
    29581 ctgaactagt cttcgccgtg cgtgaagcac ttgataaaga aggttttaat tatgtaaata
    29641 tcgtcgtaag ttctggcttt actcctgaaa aaatcaaata ttttaaagcc aaaaatgctc
    29701 ctgttaatct ctatggtgta ggctctttta ttgtgaacaa taaagaagtc ggatttactg
    29761 gtgatgttgt cgttgttaac ggcaaaccgc aggcaaaagt tggacggcaa gaatacttca
    29821 gtgataagct tgaaaaagta acacttaaaa agcgaatgta agggctttct cttctacatt
    29881 gacaatgaaa atattttcaa gtaaaattgg tatgaaag
//
LOCUS       DAOSTA010000010        28847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_1789_length_28847_cov_10.263545, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000010 DAOSTA010000000
VERSION     DAOSTA010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 28847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28847
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1789_length_28847_cov_10.263545"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..3448
                     /locus_tag="PLU08_01805"
     CDS             89..3448
                     /locus_tag="PLU08_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOQ70478.1"
                     /translation="MKQRHKITILTLLALPLILGFTKWEKREKDPFITARAMPFVYPS
                     LNGEPKFSSQFHFNHINRGTVLNNYRGAGVTVAIIDSGLNTTHEDFLDGATTNISNKS
                     AYVEETGTYYSNIQIQSVEEHGRSIIEDTHPEAAGHGTNVASTVGAMVNGVGTAGISA
                     NVTLLVLKTNYMFTEINAAIRYAADNGADIINMSLGAYEESFIDGHGKQQTGIEGAST
                     YFQTAINYAYNKGVTVIAAAGNEKTDHYSYPASNNNVIGVGALGRNTSTTIASYSNFG
                     LHNVDIVSPGSVYVADIGGVSAYTETQGTSFAAPIVASAAAIYKGKYPTATPAKIEQR
                     LKETAYDLGAAGPDATFGYGRLDLSALLVDEVPVTGVEINPTSLTLRVGDTSQVSATI
                     LPENASNKNHIFISENDAIATVNEDTGVVTAHSVGTTRIGVLTDDLFEAYTDVTVVAA
                     GTPLEITGLDIVDTTINLGVGATKTLDINLTPNNANYNDLNFVSNNESVATISSAGVV
                     TALKSGIATITASAKVGTKSDTVTINVLNITNATETYYFKDQYWGATPANWTSVKDAF
                     GYDTNRVQVTEGASGASATSPKSFTNIQGITFGVSSNASKGAGTVKVYLKETATSTTK
                     TEVGSFTISSTGGTTRRESPTIVPSINNSGFIQFEVICSTNSVYIHDIKIDYQEVVPD
                     PVAVTGVTINPKTLSLTVGENSQATFTIAPSNATNKEVTFSSLNTSVATVTSDGLITA
                     VSEGSTTIRVTTTDGNKTDDLSLTVSPAPVYEPYLNVDFSAFKKNYIFGEALALSSIM
                     AEHRASNGTITNLTGTDLTLVSGSTKELGVVPLNFSYDGLTASVNITVTNVGSYTGAV
                     AKDPLTIKGDSFAPAISTTTGTNYSSLSASCYTFAGVGLRNLDSYIMVYTSGHFRNVS
                     ALETIKSLKLDYKTGGSSASVQAFRFGTTEITSASGAAHTTLTSSAGGTSATVNAPAG
                     TTHFRIDVSSKNLQANITITFESGSTYTFTAAEQAAAYEDYFMAVTHEECLVTDVKLT
                     TWNHLKTEFDAMLPEAQNLIRTTASVEFNARYNIIVAGYGYTNFLVSGGTGLGRDLKN
                     FENKLPILIMGLGLPLSVCFYIALKKRKKTL"
     gene            3462..4556
                     /locus_tag="PLU08_01810"
     CDS             3462..4556
                     /locus_tag="PLU08_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOQ70479.1"
                     /translation="MRKRNSIKLLPVLASLLISGCRTELSSSEKSISEGTSISSEIVS
                     SETTSVTTSSEEVSATSEKSSSENGEESSITTSSEDSSSIASSEESSVSVSSEPGVPY
                     NVTFVTNCETMLDAIYTDVIYDMPLIFREGYTLAGWYLEATFINIVSFPYYVTSDVTL
                     YAKWTEGETAHFTFELHPNNNGYIVTSYGGNAINVVVPTNYNDKPIVALGEKLFYQNG
                     SIKSVVLPATLTYIGMQAFKDAVSLESIVIPSNVTELATDAFAGTISLAQVTLSSKLK
                     IIGNNAFENSAFAEVTIPSLVTEISSRAFAGNTNLLSVNLISTTPPLRFANSFEGTVS
                     TMKYYVPSSAYNSYINSPYWQAYSAQIVVK"
     gene            4591..5628
                     /locus_tag="PLU08_01815"
     CDS             4591..5628
                     /locus_tag="PLU08_01815"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOQ70480.1"
                     /translation="MKKKAITLVVLLAMLVGCSRNESSSAPSVSSEGEVSTSEVNSET
                     TSEESSVASSEELPSSEELSSEEPSSEEPPSSSENPTNYPDTPAEIEVPQSVFEYYAS
                     VDFNKTGNNLKLELYNKIKGHVSTSYNELNATMAITDRNWDLSPDPNDSDPYMVLIYA
                     SYNDTNPKKYSTRNNDVWDKEHIWAKSHGNFDNTPPAGSDMHHLRASDKRNNGSRGNL
                     DFGKANSVKSTINDYDGKPSGKTGTQTGKSGTVYEPLPQYRGDVARAMFYMATRYHND
                     TQTQLTLVDNTTSSSTGVGKFGILSTLLEWHLQDPVDEFEFRRNGLVQGYQNNRNPYI
                     DFPSLAHDVFG"
     gene            5744..7213
                     /locus_tag="PLU08_01820"
     CDS             5744..7213
                     /locus_tag="PLU08_01820"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOQ70481.1"
                     /translation="MSKVRERANLFIYFLATILIVGLTFFFLEQSIITDVTPGYYFDP
                     LTLSNQMMVTGIIVTLVSIFSFFVIRKIRLSVNGIILCIVLTIVFTMALVNLLIMPKE
                     QLLSIRFLDGTYHDINFVLPTRMRLTAIMTLVVNFSFIFFVVLVLPNHVSFLKLVLFF
                     TIAIVLIAFFAIGYSLATEWEKYVAIVKEGPGSTYQVPASFFNNRNPYASFLLNAQVM
                     LFFLYYKNLDKKRRFFYILLQIPLVLAINFTYSKTNLLLANILLIIAFLFHLVRLLGR
                     KKFIRFGIEVFLAFLLVIGFIVFRYSPKLETTNISLFLHRTLPDEMFERVPGSFDSRK
                     KLWYYAVSLMVANPIIFVFGHGPHLSRYFYHLRMQIEIEGVMTPGFGDYHNGLVEVMH
                     TFGASGLIIYALVFLFVTILLIRRARTNRSLTFFVLLSMGVFILRSQMESLAVLLFKS
                     EGIMASLTFVLPALYLLRKSHECGFSKELKVKKTRPEEA"
     gene            complement(7242..8066)
                     /gene="nagB"
                     /locus_tag="PLU08_01825"
     CDS             complement(7242..8066)
                     /gene="nagB"
                     /locus_tag="PLU08_01825"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOQ70482.1"
                     /translation="MTIFIRDNYNEMSKAAAKIIAKQIKEKPDSVIGLATGFTPIGTY
                     RELIRMHKEEGLSFKGVTAFTLYEFLGEGFDIIKPYGLDKSSARYMHEEFFKHVDIDK
                     KNIHYFDGFTDDPRRTCQEYEEKIKAAGGIDLQLLGIGRDGHWGYNEPGSSLGSRSHV
                     VALTKQTIDDNYEDFYRKVGITKDEMPHFALTMGVGTILDARKLILVASGAKKAEIVQ
                     KALEGPITAQITSTAIQLYAGEATIILDADAASMLSNIEHIKHTEAMSKKYGFRAE"
     gene            8186..9115
                     /locus_tag="PLU08_01830"
     CDS             8186..9115
                     /locus_tag="PLU08_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOQ70483.1"
                     /translation="MNILMILTPKSEVIYLSTKMRVRDAMEVLKKARFNSVPLLDESG
                     HYVGTVTEGDILWHLDQNGYEDSLDMRLKNVKRIRDYAPVSVDEEIDELIHKALGQNF
                     IPILDDRKYFIGIVTRKNLISTYIKKVDDNKEEVVHKNEVLDNLYKRRSIRRFKEGIT
                     IPQEIIDEIVKVSLVTPTAMNRRPHEIMLVDDPQVIKTLSELHQRGGQFEKAPYLILI
                     LNDDEKEPNAYLANSNCSALLISLLLAVESFDNLGGFWVSTRYPEYNRKMLNAVGVPE
                     RFSLYGMVAFGVKGEHKPANEPLNPEKIHRNKW"
     gene            9186..9740
                     /locus_tag="PLU08_01835"
     CDS             9186..9740
                     /locus_tag="PLU08_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOQ70484.1"
                     /translation="MAKNIMVVTGSVRKTGNSIMLANAFVKGARNNGNAIQKVEASHR
                     NFSPFRDKENAWRKGKPSLVEDDFNVLAPYLHVSDVLVLASPVYFGGFSGAIKVFIDN
                     LYAYVVEHRKRNLRFTHAILLAVARDDNPDTFVGITHEFKELCRQLGIEKMDIITVPG
                     VAERGDIVGHEALDRARDLGKTIS"
     gene            9752..10753
                     /gene="galE"
                     /locus_tag="PLU08_01840"
     CDS             9752..10753
                     /gene="galE"
                     /locus_tag="PLU08_01840"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOQ70485.1"
                     /translation="MKILVTGGTGFIGSHTVVALLEEGHEVVIADNLVNSQRNVVTKI
                     KRITGVKPFFYKVDVADKKSMRKVFSEHKFDAVIHFAGLKAVGESVQKPLLYYRNNLD
                     STLTLLELMKEFDVKNILFSSSATVYGTPENVPLKEEDPIGGTTNPYGTTKLIIEQIL
                     MDFVHANEGFKAVSLRYFNPIGAHPSKLLGESPKDIPNNLMPYISRVANGKLSHLNVF
                     GDDYETPDGTGIRDYVHVLDLANGHVLALKAFSFNHAYNVYNLGTGKGTSVLELVHLY
                     EKANKLKIPYVIGPRRDGDIAASYADITKAKNELGFVPHYSVKDACRHAYLFEKKQK"
     gene            10871..11776
                     /locus_tag="PLU08_01845"
     CDS             10871..11776
                     /locus_tag="PLU08_01845"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOQ70486.1"
                     /translation="MENYSHKYKISPELTLYGAYWGVKKPRANVLVITGMQEHAMRYT
                     EFAKFLNEAEFSVYSIDYFGQGENVTQGGHTLGVLPNHGFEKFVTALGTFVENLAKDK
                     LPLYVLGHSMGSFLAQRFVQRYPLLSKKVVIVGSNGPSALFGMGKAIAALTVNNRNRD
                     KESKLLASMAIGAYSKAVKNAHTPADWLSYNEDNVNTYLAHPLDGKPSSKGFYLELLR
                     GTSNLYKKKHYVDVPRDLPIFIIAGAEDPVGANGKGIIKLGKFYAKLGFTNLEVKIYE
                     GMRHEILNETDRRDVYADVLSFLMR"
     gene            11794..12276
                     /gene="smpB"
                     /locus_tag="PLU08_01850"
     CDS             11794..12276
                     /gene="smpB"
                     /locus_tag="PLU08_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOQ70487.1"
                     /translation="MAKQKKTNNVITENRRASHDYFLSDFLEVGIVLKGTEIKSLRHS
                     GASLKDTYIVFRNGEAFIEGLYIAPYAFGNIFNHEPRRTRKLLMHKREIARYSANVQI
                     ASMTCVATKMYFVRGRVKLTIALGKGKKLHDKRDAIREREDKLAMAKALKQERSSRDD
                     "
     gene            12269..13489
                     /locus_tag="PLU08_01855"
     CDS             12269..13489
                     /locus_tag="PLU08_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOQ70488.1"
                     /translation="MTKEQIKEYLTNTPTRYDKRAYDSFLKEHGIPKLPPAIHITGTN
                     GKGSTAYYLALSLQNSGYNVGLYTSPSFKNVNELACINGEEISDEYISECIKKYDEDF
                     KKYELSLFEIMTFISFNYFADNNLDYVVIEVGMGGLLDATNVFTPLISIITNIGLDHL
                     DTLGPAIMDVAEHKAGVIKKGVPVLLGDAVRRAEKVVLDTALKLNAPVYRTHNNALVT
                     RHDLERINFTYNGEHYTLYSGALYEVNNAVAALDAIEILKSLGVSIKKSAIKAAFKTK
                     PFEGRFNVIQKEPAIIVDGLHNPHAIRALLSDIKVYGKKLKVVFAAFNDKDYFQEIDL
                     LILAGATITLTTFDHPRAVRDFGSTQLPYIGEHKVAIKEAVKSLKKNDMLLIVGSLYF
                     ARLVIDEFKRGEYA"
     gene            13482..14294
                     /locus_tag="PLU08_01860"
     CDS             13482..14294
                     /locus_tag="PLU08_01860"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOQ70489.1"
                     /translation="MLKTKRLVTALTLSAFLIAISVVIQRFLVIPFGMPSLYRLSLGN
                     IPIIMASLYLGSVFGAIVGAASDLIGATLFPVGTLIIWPVISSTLYGVVPWLILRLVM
                     YLDRKIKVPLFYVFLAIIFIGLETYIFVKPSIRHPFNSTLDPIMFTTTFRIVFTLVLL
                     LIFSGLIITFNVLVKKYKEGAYEKYTGAPTSLAFTLMLMTFFVDILYSSWWKMFQFKV
                     DFFVSVFFHTLIMFILLPFQVVLLLILSNVYAKSRVAELLALPPKEHIDTDD"
     gene            14494..14622
                     /locus_tag="PLU08_01865"
     CDS             14494..14622
                     /locus_tag="PLU08_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70490.1"
                     /translation="MFEELLFTYDPRTKKEEQREDDDTLEMAQALALYEKHLEDNN"
     gene            14709..16340
                     /locus_tag="PLU08_01870"
     CDS             14709..16340
                     /locus_tag="PLU08_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOQ70491.1"
                     /translation="MNNKWWHERIFYQIYPRSFKDTNGDGVGDIPGIISKLPYLKSLN
                     VGAVWISPLYASPNFDNGYDISDYKAIHPEYGTMEDMERLLAQAKKLDIKIIMDLVIN
                     HTSFQHDWFLKSKDPKSEYHNYYFWRKGKISKKGKKLPPNNWQSMFTGPAWTYEPSND
                     MWYLHLFTREQPDLNYENPKVLEEVKSILRFWLDKGVAGFRCDVINLISKTSLKDGKK
                     RVYKTGKEHYLNAPGCHLILKELNDEILEPYAAYTVGETMDITIDAAKDYLDGELTQV
                     FAFDQVQVDYFLLPTFKRKYKPHRMVNALKKWQLKIPWNTVFYENHDLPRSVSRFGSK
                     KYYKESVTALLASVLFLRGTPYIYQGQEIGMTNVDFGGIENIEDVSSRNVYNLLRKFK
                     VPRKLAWRFVNNFTRDHGRSPVQWNDKKHAGFTTGKPWLMVNPNYTEINVEAQEIDQD
                     SVLHHYRKMATLRQKTKAFKNGDLEFKKTHRDILAFTRTDDLNEYLVVINLASKARTH
                     KLNLDGEVLYSNYQEFSDKAKKLRPFEVQIIKRLD"
     gene            16407..16886
                     /locus_tag="PLU08_01875"
     CDS             16407..16886
                     /locus_tag="PLU08_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOQ70492.1"
                     /translation="MKRKDYINWDEYFMGIAILSSKRSKDPSTQVGACIADDENKVVS
                     LGYNGMPRGIDDDVIPWEKGEDINNKYFYVCHAELNAILNTRSGASLKGCRLYVTLFP
                     CNECAKACIQVGIKEIVYLDDKYHEQVNMRASRHMLKLAGVKLTPFKGKVLDPKFNE"
     gene            16879..17694
                     /locus_tag="PLU08_01880"
     CDS             16879..17694
                     /locus_tag="PLU08_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOQ70493.1"
                     /translation="MNKYALEIMNLNYSYDKNVEVLNGVTFNVKAGTYVSVIGHNGSG
                     KSTLAKLIAGILDVGKGSVIKVFGKPLSSYDDERPDIGIVFQNPDNQFIGATVRDDIA
                     FGLENKRVAHAEMDDIINEYAAKVKMSEFLDKEPSNLSGGQKQRVAIAGVLAMKPRIL
                     LLDEATAMLDPRGKREIKEIVTELRKEVPDLTIISITHDLEETLHSDEIIVMNEGKIV
                     LQGTPEDIYKNHETLTSIRLDLPFIFRIKLELEKQGIKVKSKDLESLAEELWP"
     gene            17685..18644
                     /locus_tag="PLU08_01885"
     CDS             17685..18644
                     /locus_tag="PLU08_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOQ70494.1"
                     /translation="MAIKFHHIYFSYAPKSPFQYDALHDINLEIKQNSFTAVIGHTGS
                     GKSTLVQHINALLVPTSGAVKVEDFLILPCKKKKYLRQLNRKLKSKKVSAEQKQRLAF
                     LKSIVESGAAYKVKELRKKVGVVFQFPEYQLFEETVIKDVAFGPKNFGVSNEEALALA
                     KNALTLVGIDESFYSRSPFELSGGEKRRVAIAGILALKPEILVLDEPTAGLDPLSAHE
                     MMEVFANIHRAGTTIVLVTHDMDLVLRYASDVVVMKDGKIVEETTPQKLFADVKEEYS
                     LEVPAFYAFMQALVQRGCPLDITKINTVSDLAKMVKNLKEQAK"
     gene            18641..19525
                     /locus_tag="PLU08_01890"
     CDS             18641..19525
                     /locus_tag="PLU08_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOQ70495.1"
                     /translation="MKNISLGRYMPADSFMHKADPRIKFFGLVVFMVLIFLKFANPAM
                     DFLMYGIYAVVVFILMKVTKLKIFSLFKQLKSLWFMMIFLLIINIFTFRNSEGNILFM
                     IGNFAIKDAALYQTLYIFIRLVLMIALTSVLTASTRPLDLTYAIEWYMSPLKVIRFPV
                     HEIAMTLSIALRFIPTLLDETGRIMRAQESRGVDFKGGKVKEKLRGVVALIVPLFVSA
                     LQRSDELANAMIARGYDPQAKRTRYRILRFTWRDLIVLLLAVSFLTLVILLRVYHIDL
                     ANMLYVNIQNLLSLWGLK"
     gene            19525..20250
                     /gene="truA"
                     /locus_tag="PLU08_01895"
     CDS             19525..20250
                     /gene="truA"
                     /locus_tag="PLU08_01895"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOQ70496.1"
                     /translation="MRYVAIVKYLGTNYFGWQRQPFEISVQQVIEDKLSTIFNTEIVI
                     YASGRTDARVHAYGQVFHFDAPPFPVSKLKYALNKMLPGDIEIVKLQKAKDDFHARYA
                     AKSKTYEYLIYLGAKDPFLFNRVLFYPFALDEKKLRKDAKLFVGTHNFQNFTSKKEDE
                     ANFVRNISAVKIQKSGKMLKIVFIGEGFMRGQIRMMVGALLAQNAGKEDANFIKDNLN
                     SKERKIISHKVIGDGLYLRKVKY"
     gene            20286..21002
                     /locus_tag="PLU08_01900"
     CDS             20286..21002
                     /locus_tag="PLU08_01900"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ70497.1"
                     /translation="MGRAFQVRAASMASTAAKKSALNMRASKEIFMVAKSGGTDPNSN
                     LALRAALDKYRAMGATKDVMERAIKKAEGDDAFAYIPGRYEVMGPGGSQLVIDTLTDN
                     VNRAISDIKAVLKKTDSILQTVAYNFTETGIFLFAGNNLEEITETLILNDVDVWDINE
                     EDDVIEVLVNPTSYGKTRDTLYSLGIKEFNISEIQMIPNDKVTLNAEDTEVFKRIIDM
                     LEDVQDVQEVYHNVNVPTEE"
     gene            21041..21799
                     /locus_tag="PLU08_01905"
     CDS             21041..21799
                     /locus_tag="PLU08_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOQ70498.1"
                     /translation="MRRNANPIFRNMNNDMNQAMTSSVPVDVTVSTASWSGIALKTLL
                     LVLIMIAAGLSTWFLPFPVVVGFLIFGGIASFFFVFFAMRSVKTAPVLSVLYAITQGI
                     MYGALTFIVETYFPGVGVMALVATFGIVGVMAFLHSIKAVRATPGLIRFVFAALLMVM
                     ISSLVLFIISRVAQDLYLSYVDNYPLMVIVSAFLVVLGAIMLILDFNNAETIVQSGAP
                     KIYEWQVSLGFLVTLVWIYLQVIRFLLVLARNRE"
     gene            21936..22877
                     /locus_tag="PLU08_01910"
     CDS             21936..22877
                     /locus_tag="PLU08_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ70499.1"
                     /translation="MINMIKVKNLHKTYKKGVKAVNGLNFKVKAGEIFGLLGPNGSGK
                     TTTINAILDLVDYETGEIILFDNKYKTKVEAKKHIGLITQEVGVFNELTVRENIDYFC
                     GLYINDEKLRAQYVEEAMDFVQIKDFAKFKPKKLSGGLLRRLHIACGIAHKPTLIFFD
                     EPTVGIDPQSRNHILEQIKVLNKNGATIVYTSHYMEEIEMLCDYILIMDKGQVIAEGN
                     YDALIDQIDLAEYIEIEYKGEMPLKVVTEINELPGILEVIHTKEKLVIKTSEDDKAFI
                     KIIHILENNNVDYSRANANKPSLNDVFLELTGKELRD"
     gene            22879..24045
                     /locus_tag="PLU08_01915"
     CDS             22879..24045
                     /locus_tag="PLU08_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ70500.1"
                     /translation="MKSLFILNLKKLLKNKEIVFWALLFPIILSTIFHIVFDGIRNRD
                     NFETLKVNYVSTNFTAEDKAFLVEEMLTNKAEYIKNEGTDKAQTVKIFALSEKTKAEA
                     EKEFKKDKTIYFYKEDGAFKIKASSQNISTAILQTFIDDYQNFIKVTTLLVAESNGTL
                     SFQDAMNKHVASYAYTYDFDSGDKKIDYVNNYHYTVLAMAIIYGAFLATEQAKIFQPN
                     SFAFAKRVTVSGASRAKLLVSALLSSLVVQTAIIALYIAILVFIFKVTFANIGLALLA
                     ALIGVILVNINGFFIGIVFNKLSQNAVIAIIILAGTIGGFLSGMMISSMKFFVGTKMP
                     LLSYININALISDSFVSLDFGNMTRYWNNMLAMTVIGVILFGVTLIKFNRESNK"
     gene            24047..25282
                     /locus_tag="PLU08_01920"
     CDS             24047..25282
                     /locus_tag="PLU08_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ70501.1"
                     /translation="MGFKLFFKIVKANFVSFLAYVLILVVSGLMFGLSDSGAANEDFK
                     ILAPVYYANITNETVDDLPSDNTLIFDYLMDNNLISEESDDPFSQGFYTYTLNFVNVK
                     KNIKPSEVNDAIYAGVVRGALIIPKDLSTLTRENVLFIFHTNSQDLATITPTKAALNK
                     FINTYHDLKEVSATAGNNLSATELTNKTLIALEKETEIVYENPNRTKLSMLSHFFSFS
                     TYIMSAVIILLVSIVMYEIKRREILMRISITPYSIPRLIVGLIFGAITLSLLLVGLVY
                     VLAIISYPKIALSITGLYLTLNIMLFTLPLTALAMVIGLVISKAEIVSIISTVFALAQ
                     AFFSGAFLPTFLLPKSIINVGKVFPASHTVTINNLLAEGLNTNGPSILLNIGILLAYF
                     AVFLTIAIILSKKITKREG"
     gene            25434..25889
                     /gene="rplM"
                     /locus_tag="PLU08_01925"
     CDS             25434..25889
                     /gene="rplM"
                     /locus_tag="PLU08_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOQ70502.1"
                     /translation="MLRQTTHANAATVERKWYVVDATDLPLGRLASEIAMTLMGKNKP
                     EYTPHVDCGDYVIVVNADKVSLSGNKLKTKKYYNTSQRPGGLRVRTAGEMLANYPVEM
                     MERAVWGMVPKGRLGRQIIKKLFVYAGPEHKHSAQQPVELPLRYIGGSK"
     gene            25889..26287
                     /gene="rpsI"
                     /locus_tag="PLU08_01930"
     CDS             25889..26287
                     /gene="rpsI"
                     /locus_tag="PLU08_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOQ70503.1"
                     /translation="MAKRKAETYLGTGRRKSSVARIILKPGKGSIIVNDQDVNDYMPY
                     QTLVMDLKQPLTLLDVADKYDVIANVHGGGFTGQAGAIRLGIARALLEAGLDRGTLKR
                     AGMLTRDARAKERKKYGLKGARRAPQFSKR"
     gene            26417..26493
                     /locus_tag="PLU08_01935"
     tRNA            26417..26493
                     /locus_tag="PLU08_01935"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26451..26453,aa:Ile,seq:gat)
     gene            26599..28500
                     /locus_tag="PLU08_01940"
     CDS             26599..28500
                     /locus_tag="PLU08_01940"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HOQ70504.1"
                     /translation="MKQELIKNNYYEAVNKEWIDAAVIPGDQPMMSAFLELHLDIEKL
                     LMDLAKKWDKDQTGLNNHLQEFVKLYKMTNDFATREKLGAEPLKPVIKKILNLKSLKD
                     LEKNMKDFVLDGVDLPFSFMVFQDFKDSNNEVLYFTAANLFLPDTSYYENEETKQQLI
                     GLFTMTTMQVLSLYGLEEEAAQDLLNKALAFDAMLVPVTMSSVERADMIKMYNPLLKD
                     EFASKTKTFNLLKVAEALVGQDVDKVVAMNLDFIDKFEEIVNDANFELIKAWMVVSNI
                     LTFSAELTEELRVAGGAFGRALQGIAEAQSQEKAAFYQAYDRLSQAVGLYYGLTYFGP
                     EAKKDVEDMVNTIIGVYKDRINKNTWLNAETKAKAILKLNTMKVHIGYPRELPKYYDK
                     YKVGSYAEGSNLVKEKLKYSRLVQKENFKKYNKKPNRNLWAMPASMVNAYYQPMNNQI
                     VFPAAILQEPYYSLSQGRSANYGGIGAVIAHEISHAFDNNGAKFDEYGSLNNWWTDED
                     LKTFEEKAQKMIALFDGVESGYGPCNGTLTVSENIADSGGIRCALEASKKEKDHNYEE
                     FFKNWARVWRQKSSPEFMQLLLTVDVHSPSNLRANMQLKNLVEFQEFYNLTEQDEMYL
                     AKEQMVEIW"
     gene            28595..28670
                     /locus_tag="PLU08_01945"
     tRNA            28595..28670
                     /locus_tag="PLU08_01945"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28628..28630,aa:Val,seq:cac)
     gene            28681..28756
                     /locus_tag="PLU08_01950"
     tRNA            28681..28756
                     /locus_tag="PLU08_01950"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28714..28716,aa:Thr,seq:tgt)
     gene            28763..28847
                     /locus_tag="PLU08_01955"
     tRNA            28763..28847
                     /locus_tag="PLU08_01955"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28797..28799,aa:Leu,seq:tag)
BASE COUNT         9456 a         4678 c         5663 g         9050 t
ORIGIN      
        1 aaaataatgt tttgttacac aaaaaactta ttatttatat attttgaata gtttcatgtt
       61 attatcttaa ttactggaga tttttactat gaaacaacgt cacaaaataa caatcctaac
      121 tttgttagcc ttaccactta ttcttggttt tactaaatgg gaaaaaagag aaaaagatcc
      181 gtttattaca gcacgggcta tgccttttgt gtatccgtct ttaaatggtg aaccaaaatt
      241 ttctagtcaa ttccatttta atcatattaa tcgcggcact gtacttaata actatcgtgg
      301 tgcaggagtc acagtagcaa ttattgactc aggactaaat acaacacatg aagatttttt
      361 agatggcgct actacgaata tttctaataa atcagcgtat gttgaagaaa caggaactta
      421 ttattcaaat atacaaattc aatctgtaga agagcatgga cgaagtatca ttgaagatac
      481 tcatcccgaa gctgcggggc acggcacgaa tgtcgctagt acagttggtg cgatggttaa
      541 tggtgttgga acagcgggta tttcagcaaa tgtaacactc ttggtgttaa agacaaatta
      601 tatgtttact gaaattaatg cggcaattcg ttacgctgct gataatggtg ccgatattat
      661 taatatgtca ctaggcgcat atgaagaatc attcattgat ggccacggga aacaacaaac
      721 tgggatcgaa ggtgcttcta cctacttcca aacagcgatt aattatgctt acaataaagg
      781 tgtaacagta attgcggcag cgggaaatga aaagactgat cattattctt atcctgcgag
      841 taataataat gttattggtg ttggtgcact tggtcgtaat acatcaacaa ccatcgcttc
      901 ttattcaaac tttggcttac ataatgttga tatagttagt ccaggaagtg tttacgtcgc
      961 tgatattggt ggcgttagtg cttacacgga aacacaaggc acatcatttg cagccccaat
     1021 tgtggcaagt gcagcggcta tttacaaggg aaaatatccg actgcgactc ctgctaaaat
     1081 tgaacaaaga ttaaaagaaa cggcctatga cctaggtgca gcgggaccag atgctacttt
     1141 tggttacggt cgtcttgatt taagtgcctt acttgttgat gaagtaccag ttactggtgt
     1201 tgaaattaat ccaacaagtt taacacttag agttggtgac actagtcagg taagtgcaac
     1261 aattcttcct gaaaacgcta gtaataaaaa ccatatcttt attagtgaaa atgatgctat
     1321 tgcaaccgtt aacgaagata ctggagtagt aaccgctcat agtgtaggaa caacaagaat
     1381 tggtgtactt actgatgact tattcgaagc ttacactgat gttactgttg ttgctgctgg
     1441 gacaccgcta gaaataacag gtctagatat tgtcgataca acaattaatt taggagttgg
     1501 tgcgactaaa acactagata ttaatttaac tccaaacaat gcaaattata atgacttaaa
     1561 ttttgttagt aataatgaaa gcgttgcaac tataagtagt gctggtgttg tcaccgccct
     1621 taaaagcgga atcgctacga ttaccgctag tgctaaagtt ggaacaaaga gtgatactgt
     1681 tactattaat gttttaaata taaccaatgc gacagaaact tattatttta aagatcaata
     1741 ttggggcgct actcccgcta attggacaag tgttaaagat gcttttggtt atgatacaaa
     1801 tcgtgtacaa gtaactgaag gcgctagtgg agcaagcgcg acatcaccaa aaagctttac
     1861 aaatattcag ggtattactt ttggagtgtc gtctaacgct agcaaaggcg caggtactgt
     1921 taaagtgtat ttaaaagaaa cagctacttc cactacaaaa acagaagtag gtagttttac
     1981 gataagtagt actggcggaa caactaggcg tgaaagtcca actattgtcc caagtataaa
     2041 taatagcggt tttattcaat tcgaagtgat ttgttcgaca aatagcgtgt atattcatga
     2101 tattaagatt gattatcaag aagtagtacc tgatccagtc gctgtcactg gtgtgacgat
     2161 taatccaaaa acattgtcat taactgttgg cgaaaatagt caagcaacat ttacgattgc
     2221 accaagtaat gctacgaata aagaagtaac attttcaagc ttaaacacta gtgttgctac
     2281 tgttacgagt gatggtctaa taactgctgt aagcgaaggg agtacgacaa ttcgcgttac
     2341 tactacagat ggtaataaga ctgatgactt aagtttaaca gtttcaccag caccagttta
     2401 tgaaccatat ttaaatgttg attttagtgc cttcaaaaag aattatattt tcggtgaagc
     2461 tttagcgctt agttcaatta tggcagaaca tcgcgcaagt aatggcacga ttacaaactt
     2521 aacaggtaca gatttaacac ttgttagtgg ttccactaaa gaattaggtg ttgtgccact
     2581 taactttagt tatgatggcc ttactgcaag tgttaatata actgtgacta atgtcggaag
     2641 ttatactgga gccgttgcaa aagatccgct aacaattaaa ggtgattcat ttgctccagc
     2701 aatttcaaca acaacgggca caaattatag tagtttatca gcatcctgtt atacttttgc
     2761 tggtgttgga ttacgtaatt tagattcgta tattatggtc tacactagtg gtcacttccg
     2821 caatgtttca gcacttgaaa cgattaaatc acttaaatta gactataaaa ctggtggaag
     2881 tagtgcttca gtacaagctt ttagatttgg tacaactgaa atcacaagtg caagtggtgc
     2941 agcacacacg actttaactt catcagctgg aggcacaagc gcaacggtaa acgctccagc
     3001 gggtacaaca cactttagaa ttgatgtaag tagcaaaaac ttacaagcaa atataacgat
     3061 tacctttgaa tcaggtagta catacacatt tacagctgca gagcaagcag cagcatacga
     3121 agattatttc atggctgtaa ctcacgaaga gtgtcttgtc actgatgtaa aacttacaac
     3181 atggaatcat ttaaaaactg aatttgatgc gatgctacct gaagctcaaa atttaattag
     3241 aacgaccgct agcgtggagt ttaatgctcg ttataacatt attgttgcag gttatgggta
     3301 cactaatttc ttagttagtg gtggtacggg actaggtcgc gatcttaaaa atttcgaaaa
     3361 caagctacca attctaataa tgggattagg attaccatta tcagtttgtt tttatattgc
     3421 acttaaaaaa agaaaaaaaa cattataatt taagtaaggt tatgagaaaa agaaattcta
     3481 taaaattatt acctgtttta gcgtcactac ttattagtgg ttgtcgaact gagttaagta
     3541 gtagcgaaaa aagtataagc gaaggaacaa gtattagttc tgaaattgtt tcaagtgaaa
     3601 ctacaagtgt gacgacttct agcgaagaag taagcgcaac ttcagaaaag tcgtctagtg
     3661 aaaacggcga agaatcgtca attacgacga gtagcgaaga ctcttcaagc atcgcaagta
     3721 gtgaagaatc ttctgtttct gtaagcagcg aaccaggagt gccctataat gttacatttg
     3781 ttacaaattg tgaaacaatg cttgatgcaa tctacaccga tgtaatttat gatatgcctc
     3841 ttatttttag agaaggctac actcttgcag gatggtactt agaagcaaca tttattaata
     3901 tcgtcagttt tccttattat gtgactagtg atgtgacatt gtacgcgaaa tggaccgaag
     3961 gtgaaacagc acactttacc tttgaattac atcctaataa taatggttat attgttacat
     4021 cttacggtgg taatgcgatt aatgttgttg tgcccacaaa ttataatgat aaacctatcg
     4081 tggcactagg tgaaaagctt ttctatcaaa atggtagtat taagtctgtc gttttacctg
     4141 ccacattaac ttatattggg atgcaagctt ttaaagatgc agttagttta gaaagtattg
     4201 ttattccaag caatgttact gagttagcaa cggatgcttt tgccggaaca ataagcctag
     4261 cgcaagtaac actttcgagc aaattaaaaa ttattgggaa taacgctttt gaaaatagcg
     4321 cttttgcaga agtgactatt cctagtttag ttactgaaat tagttcacgt gcttttgctg
     4381 gtaacactaa tttactaagt gttaatttaa tatcaaccac accaccatta cgtttcgcta
     4441 attcatttga aggcactgtt tcgacgatga aatattatgt gccttcctca gcatataact
     4501 catatataaa tagcccttat tggcaagcat attccgcaca aattgtggtt aaataaatag
     4561 aaaggtctaa aaataaatag gtaaaaaagt atgaaaaaga aagcaattac acttgtagta
     4621 ctattagcaa tgttagtggg ctgttcgcgt aatgaatcaa gtagcgcacc ttctgtaagc
     4681 agtgaaggag aagtgtccac tagtgaagtt aatagtgaaa cgacaagtga agaatcaagt
     4741 gttgcgagta gcgaagagct gccaagtagt gaggaacttt caagcgaaga accttcgagt
     4801 gaagaaccgc ctagtagcag tgaaaacccg actaattatc ctgatacacc agcggaaatt
     4861 gaagtccccc aaagtgtgtt tgaatattac gcaagtgtag actttaataa aacaggcaac
     4921 aatttaaaac tagaactata taacaaaatt aaagggcatg taagtacaag ttataacgaa
     4981 ttaaacgcaa caatggctat tactgatcgt aactgggact tatcaccgga ccctaatgat
     5041 agtgatccat atatggtgct aatttacgct agttataatg atactaatcc taaaaaatat
     5101 tctacccgta ataatgatgt atgggataaa gaacatattt gggcgaagtc tcacggtaac
     5161 tttgataata ccccaccagc gggaagtgac atgcatcact tacgcgctag tgataagcgc
     5221 aataatggta gccgtggtaa tttagacttt ggtaaggcaa atagtgttaa atctacgatt
     5281 aatgattatg atggtaagcc ttctggtaaa acgggaacgc aaaccgggaa aagtggaacc
     5341 gtttatgaac cactaccaca atatcgcggt gatgttgcgc gtgcgatgtt ttatatggca
     5401 acacgttacc ataatgatac gcaaacgcaa ttaacacttg tcgataatac aacaagtagt
     5461 tcaactggag taggtaaatt tggcatttta tctactcttt tagaatggca cttacaagac
     5521 ccagttgatg aatttgaatt taggagaaac ggattagtgc aaggttatca aaacaaccgt
     5581 aatccgtata ttgacttccc atctttagcg catgatgtat tcggttaata aagaaaatta
     5641 tagcaaaaaa acacaaataa aatacacaca taaaatgtgt gttattttaa ccgcacaatt
     5701 atctgcatta acatttagga acaaatagtt gtataattaa aaaatgagta aagtcaggga
     5761 aagagcaaat ctgtttatat actttttggc cacaattcta attgtgggct taactttctt
     5821 ctttttagaa cagtcaataa ttacggatgt tactcctgga tattactttg atccgctaac
     5881 actttctaat caaatgatgg taacaggaat cattgtaaca ctcgttagta ttttttcttt
     5941 tttcgttatc cgtaagattc gtcttagtgt aaacggtatt atcctgtgta ttgtacttac
     6001 tatagtattt actatggcac ttgttaactt gctaataatg ccaaaagaac aattgctttc
     6061 aattcgcttt ttagatggaa catatcacga tataaacttt gtgttaccaa caaggatgcg
     6121 tttaacagcg attatgacgc ttgttgtgaa ttttagtttt attttctttg tcgtgcttgt
     6181 gttaccaaat catgtgtcat ttttaaaatt agtgcttttc tttactatag caattgtctt
     6241 aatcgctttt tttgctattg ggtacagtct tgctactgaa tgggaaaaat atgttgcaat
     6301 tgtaaaagag ggtccaggga gcacttacca agtgcccgct agttttttta ataacagaaa
     6361 tccttatgcg tcgtttttgc tgaatgcgca ggtaatgctc ttttttctct attataaaaa
     6421 tcttgataaa aaacgacgtt tcttttatat tttactgcaa attccgcttg ttttagctat
     6481 taacttcact tattctaaaa caaatttatt attagctaat atactgctta ttattgcttt
     6541 tttatttcat ctggttaggt tattaggtcg caagaagttt attcgctttg gtattgaagt
     6601 gtttttagca tttttactcg taattggatt tattgtcttt cgttattcgc caaaattaga
     6661 aacgacgaat atttctttat ttttacatcg gacactccct gatgaaatgt ttgaaagggt
     6721 gcctggctct tttgattcac ggaagaaact ttggtattac gcagttagtc ttatggtcgc
     6781 taatccgata atatttgtct ttggtcacgg tccacatctt tcgcgctatt tctatcactt
     6841 acgaatgcaa attgaaatag aaggagtaat gacaccgggc tttggtgatt atcataatgg
     6901 cttagtcgaa gtaatgcata cttttggcgc aagcggatta ataatatatg cccttgtttt
     6961 tctctttgta acgatattac taattagaag agcaaggaca aatcgcagtt taactttctt
     7021 tgtcttgctt agtatgggcg tctttatttt acggagtcaa atggaatctt tagcagttct
     7081 cctttttaaa agcgaaggta ttatggcttc actaacattc gtattacctg cgctttattt
     7141 attaagaaag tcgcatgaat gtggctttag taaagaatta aaagttaaaa aaactaggcc
     7201 agaagaggcc tagtcatttc ttaaaatatg attaataagt attattcggc acggaagccg
     7261 tattttttac tcattgcttc ggtatgttta atgtgttcaa tatttgataa catacttgcg
     7321 gcatcagcgt ctaaaataat tgttgcttcg ccagcatata actgaatagc agtagaagta
     7381 atttgggcgg tgattggtcc ttcaagtgct ttttgtacaa tttctgcttt tttagctccg
     7441 ctagcgacaa ggattaattt tcttgcatct aaaattgtgc caacgcccat tgttaaggca
     7501 aagtgcggca tttcatcttt agtaattcct actttccgat aaaagtcttc atagttatca
     7561 tcaattgttt gtttagttaa agcaacaaca tggctacgac tacctaaact tgagcctggt
     7621 tcgttataac cccagtggcc atcacggccg atacctaaga gttgtaagtc aattccgcct
     7681 gctgctttaa ttttttcttc atattcttga caggtgcgac gtggatcatc ggtaaatccg
     7741 tcaaaataat ggatattttt cttatcgatg tcgacatgtt taaaaaattc ttcatgcatg
     7801 taacgagcgc ttgatttatc taatccgtaa ggcttaataa tatcaaaacc ttcacctaag
     7861 aattcataaa gggtaaaagc tgttactcct ttaaaactta aaccttcttc tttatgcatc
     7921 cgaattagtt cacgataagt gccaattggt gtaaagcctg tcgcaaggcc aataactgaa
     7981 tcaggttttt ctttaatttg cttagcaatt atttttgctg ctgcttttga catctcgtta
     8041 taattgtcac gtataaaaat agtcataata ttctcctcat aaatagtcta acatatagtt
     8101 acaccatata atacaacaaa gttccttttt ttaccacagt gaaagcggtt ttattatata
     8161 attatagtat agaaagaggt ggagcatgaa cattttaatg atactaaccc cgaagagtga
     8221 agtcatatat ttatcaacta aaatgcgtgt aagagatgcg atggaagtgt taaagaaagc
     8281 acgatttaat agtgtacctt tacttgatga aagtggtcat tatgttggca ctgtcactga
     8341 aggtgacatc ttatggcatc ttgatcaaaa tggttatgaa gattcacttg atatgcgctt
     8401 aaaaaacgtt aaacgcatta gggattacgc tcctgtatca gtggacgaag agattgatga
     8461 attaattcat aaagcactcg gtcaaaactt tattccaatt cttgatgatc gaaagtactt
     8521 tattggtatt gtgacacgga agaacttaat tagcacttat attaaaaagg ttgatgataa
     8581 taaagaagaa gttgttcata aaaatgaagt gctcgacaat ttatataaaa gaagaagtat
     8641 tagacgcttt aaagaaggca taactattcc acaagaaata atcgatgaaa ttgttaaagt
     8701 tagtttagta accccaacgg caatgaatcg acgcccacat gaaataatgt tagtcgatga
     8761 tccacaagtt atcaaaacac ttagcgaatt acatcaaaga ggcggccaat ttgaaaaagc
     8821 tccatattta atattaatat taaatgatga tgaaaaagaa ccaaatgctt atcttgcaaa
     8881 tagtaactgc agtgctttac ttattagctt attactcgct gttgaaagtt ttgataattt
     8941 aggtgggttc tgggttagta cccgttatcc agaatataac aggaagatgc taaacgctgt
     9001 tggtgtacca gaacgcttta gtctctacgg gatggttgcc tttggagtca agggtgaaca
     9061 taaaccggca aatgaaccac ttaacccaga aaaaattcat cgtaacaagt ggtaaaaact
     9121 taataaaagg cacgatttta agcacaatat aacaatttta tgatataatt attgatggag
     9181 gcaatatggc aaaaaacatt atggtagtta caggcagcgt ccggaaaaca ggcaactcaa
     9241 ttatgcttgc aaatgctttt gttaaaggcg cgcgaaataa tggtaatgcg attcaaaagg
     9301 ttgaagcttc gcatcgtaat ttttcaccat ttcgcgataa agaaaatgca tggcggaaag
     9361 gtaaaccaag tttagtcgaa gacgatttta atgtccttgc cccatattta catgtcagtg
     9421 atgttttagt gcttgcttca cctgtttact ttggtggctt tagcggcgca atcaaagtat
     9481 tcattgataa tctttatgct tatgttgtcg aacatcgtaa acgcaactta cgctttacgc
     9541 atgcaatact ccttgcggta gcgcgtgacg ataatcccga tacatttgtt ggtattaccc
     9601 atgaatttaa agaattatgt cggcagttag gtattgaaaa aatggatatt attactgtac
     9661 ctggtgtagc tgaacgtggt gatattgttg ggcatgaagc acttgaccgt gcgcgggatt
     9721 taggaaaaac aattagttag gaaatagttt tatgaaaata ttagttactg gaggcactgg
     9781 cttcatcggt tcacataccg ttgtggcact actagaagaa ggtcatgaag tcgttattgc
     9841 tgataattta gtgaactcac aaagaaatgt cgtcactaaa attaaacgga ttacaggagt
     9901 aaagccgttt ttttataaag tcgatgttgc tgataaaaaa agcatgcgaa aggttttcag
     9961 cgaacataaa tttgacgctg tgattcattt tgctgggctt aaagcggttg gtgaaagtgt
    10021 gcaaaaaccg ctcctttatt acagaaacaa cttagattca acattaacac tgctggaact
    10081 gatgaaagaa tttgatgtga aaaacattct tttttccagt agtgcaacgg tatatggcac
    10141 ccctgaaaac gtcccgctta aagaagaaga cccaatcggc ggtactacca atccctatgg
    10201 cactacaaag ttaattattg agcaaatctt aatggatttt gtgcacgcta atgaaggttt
    10261 taaagcagtt agtttacgct attttaatcc aattggtgct catccttcaa aattacttgg
    10321 tgaatcacca aaggatattc ccaataactt aatgccttac attagccgtg tcgctaacgg
    10381 gaaattaagt cacttaaatg tgtttggtga tgattatgag acgcctgatg ggacaggaat
    10441 tagagattat gtccatgtcc ttgatttagc taatggtcat gttttagcgc ttaaagcctt
    10501 tagtttcaat catgcttata atgtttataa cttagggaca ggcaaaggca ctagcgtcct
    10561 cgaattagtg catctttatg aaaaagcaaa taaacttaaa attccgtatg tcattggtcc
    10621 acgtcgtgat ggtgatattg ccgcaagcta cgctgatatt acaaaagcta aaaatgaatt
    10681 aggctttgtt ccgcattatt cagtaaaaga tgcttgtcgc cacgcttatc tatttgagaa
    10741 aaaacaaaag taataatttt tatatttata tcaaaaaatt aaggagtaat ggttaaccat
    10801 aaatattttc attttactta tgtaagcaat atgtaaataa atggtaaaat taaaagaagg
    10861 aggacgtaat atggaaaatt acagccacaa atataaaatc tcccccgaac ttacacttta
    10921 tggtgcttat tggggtgtta aaaaaccgcg ggcgaatgtc ttagtcatca cagggatgca
    10981 agaacatgca atgcgctaca ctgaatttgc aaaattctta aatgaagcgg aatttagtgt
    11041 atatagtatc gactattttg gacaaggaga aaatgtcacg caaggtggtc atactttagg
    11101 tgttttacct aatcacggct ttgaaaaatt tgtgacggcg cttggtactt ttgtagaaaa
    11161 cctagcaaaa gataaattac cactttatgt tttagggcat tcaatgggta gtttcttagc
    11221 gcaacgcttc gtgcaacgtt atccgcttct ttctaagaaa gtggtaattg ttggatcaaa
    11281 cggtccttct gcactttttg gaatggggaa agctattgcc gctctcaccg ttaataatcg
    11341 taaccgtgat aaagaaagta agttacttgc tagtatggca attggtgctt actcaaaagc
    11401 tgttaaaaat gcgcatactc ccgctgattg gcttagttat aacgaagaca atgtcaatac
    11461 ttatctcgcg catccgcttg atgggaaacc gtcatcaaaa ggtttttatt tagaattatt
    11521 acgcggcacg agtaatttat ataagaaaaa gcattatgtt gatgtcccgc gtgacttacc
    11581 aatctttatt attgctggtg ctgaagaccc agtaggcgct aacgggaaag gtattattaa
    11641 actaggtaaa ttttatgcaa aacttggttt tactaattta gaagtgaaga tttacgaagg
    11701 aatgcgccac gaaatattaa atgaaacaga tcggcgtgat gtttacgctg atgtcttaag
    11761 tttcttaatg agataaaaga aagatacact atagtggcga agcaaaaaaa gacgaataat
    11821 gttataacag aaaaccgccg tgcaagtcac gattattttc ttagtgactt tttagaggtg
    11881 ggaattgttt taaaaggcac agaaattaag tcgcttcgcc acagtggtgc ctcgcttaaa
    11941 gatacttata ttgttttccg taacggagaa gctttcattg aagggctata tattgctcca
    12001 tatgcttttg gtaatatttt taatcacgaa ccacgcagga cacgtaaact tttaatgcat
    12061 aaacgcgaaa tagcacgcta tagcgcaaat gtgcaaatag cgtcaatgac atgcgtcgcc
    12121 acaaaaatgt attttgttcg tggtcgtgtc aagcttacaa ttgcgcttgg taaaggcaaa
    12181 aaacttcatg acaagcgtga tgcaattaga gagcgcgaag ataaattagc gatggcaaag
    12241 gctttaaagc aagaaagaag ttcgcgtgat gactaaagag caaattaaag agtatttaac
    12301 taataccccg actcgttacg acaaaagagc gtatgactca tttttaaagg aacacggaat
    12361 cccaaaatta ccgcctgcaa ttcatataac agggactaac gggaagggaa gtactgccta
    12421 ttaccttgcc ttaagtttgc aaaatagtgg gtataatgtc ggcttatata catccccatc
    12481 ttttaaaaat gttaacgaat tagcgtgcat taatggtgaa gaaattagtg atgaatatat
    12541 tagtgaatgt attaaaaagt atgacgaaga ctttaaaaaa tatgaattat cactttttga
    12601 aataatgaca tttattagct ttaattattt tgcggataat aatcttgatt atgtcgttat
    12661 tgaagtggga atgggcggtt tacttgacgc tacgaatgtt tttaccccgc ttataagcat
    12721 cattactaat attggacttg accatcttga tacacttgga ccagcaatta tggatgttgc
    12781 tgaacataag gcgggcgtta taaaaaaagg tgtgccggtc ttacttggtg atgctgttag
    12841 acgcgctgaa aaggttgtcc ttgacactgc tttaaaactt aacgcgccag tgtatcgcac
    12901 gcataataat gcacttgtta caagacatga tttagagcgg attaatttta catacaatgg
    12961 tgaacattac acgctttata gcggagcact ttatgaagta aataatgcag tagcggcact
    13021 tgacgccatt gagatattaa aaagtttagg tgttagtatt aaaaaaagtg cgattaaagc
    13081 tgcatttaaa actaaaccgt ttgaaggtcg ttttaatgtt attcaaaaag aaccagcaat
    13141 tattgttgat ggtttacata acccacatgc aattcgtgct ttattaagtg atattaaagt
    13201 atatggtaaa aagctcaaag tagtgtttgc tgcttttaat gataaagatt attttcaaga
    13261 aattgattta ttaattttag caggggccac aattacttta acaacttttg atcatccgcg
    13321 ggctgtcaga gattttggat caacacaact tccgtatatt ggtgagcaca aagtggcaat
    13381 taaagaagca gtgaaatcat taaagaaaaa tgacatgctt ttaattgttg gttcgcttta
    13441 ttttgcgcgc cttgttattg atgaatttaa aagaggtgaa tatgcttaaa acgaaacgac
    13501 tcgtgacagc attaacacta agtgcttttt taattgctat cagtgtcgtt attcaacgct
    13561 ttttagtaat tccttttgga atgccttcac tttaccgctt atcacttggt aatattccga
    13621 ttattatggc ttcactttat cttggctccg tatttggtgc gattgtgggc gcggcaagtg
    13681 atttaattgg ggcgacactt ttcccggtcg gaacccttat tatttggcca gtgattagtt
    13741 caacacttta tggtgttgtg ccgtggctta ttttacgcct agtgatgtat ttagatagaa
    13801 aaatcaaagt accgcttttc tatgtatttt tagctattat tttcattggc ttagaaacat
    13861 atatctttgt taagccttca attagacacc cgtttaatag cacgcttgac ccgattatgt
    13921 ttacgacaac ttttagaatt gtttttacgc ttgttttact ccttatcttt agcggactca
    13981 ttattacttt taatgtgctt gttaaaaaat ataaagaagg tgcttatgaa aaatatactg
    14041 gtgcaccaac ttcactagcg tttacactga tgctgatgac tttctttgtc gatattcttt
    14101 attcgtcatg gtggaagatg ttccaattta aagttgattt tttcgtcagt gtctttttcc
    14161 acactttaat catgtttatt ttattaccat tccaagttgt gcttttatta attttaagta
    14221 atgtatacgc caaaagcaga gtagcagaac ttttagcgct acccccaaaa gaacatatag
    14281 atacagacga ttaagccttt ttataatgtt ttaaacctta agttaggcgt gaattaacgc
    14341 cttttttaaa aactactaaa aaactcctaa acgcattaga cacaatatgt caaaattaat
    14401 acttatagta ttaattatta gtgtcttaaa tacctttgaa ttttaggggc tattatctta
    14461 caatagtcgt agttaaaaaa ggagtaaatg cttatgtttg aagaactttt atttacgtac
    14521 gatccgcgaa caaaaaaaga agaacaacgc gaagatgatg acacgctgga aatggcacaa
    14581 gcgcttgctc tttatgaaaa gcatttagaa gataataact aataaaagtt attgtgactt
    14641 ttaataaatt acgccttagg gcgttttttt attgcttttt tgatacacta aaagagaggt
    14701 acaaaattat gaataataaa tggtggcatg aacgtatttt ctatcaaatt tatccacgga
    14761 gttttaaaga cacaaatggt gatggtgtcg gtgatattcc tgggattatt tctaaattac
    14821 catatttaaa atcactaaat gttggggcag tatggatttc tccgctttac gcttccccaa
    14881 attttgataa tgggtacgac attagtgatt ataaagcaat ccatccagaa tatggcacga
    14941 tggaagacat ggagcgatta cttgcgcaag ctaagaaact tgacattaaa attattatgg
    15001 acttagttat taatcatact tccttccagc atgattggtt tttaaagagc aaggatccca
    15061 aaagtgaata tcataactat tacttttggc gtaaaggtaa aattagtaaa aaaggtaaaa
    15121 aattaccgcc gaataattgg caatcaatgt ttactggacc agcctggact tatgaaccaa
    15181 gtaatgacat gtggtactta catcttttta cacgagaaca acctgattta aattatgaaa
    15241 acccaaaagt actagaagaa gttaaaagta ttttacgctt ttggcttgat aaaggtgtcg
    15301 caggtttccg gtgtgatgtt attaatttaa ttagtaaaac ttcacttaaa gacgggaaga
    15361 agcgcgtgta taaaacagga aaagagcatt acttaaatgc acctgggtgt cacttaatct
    15421 taaaagaact taatgatgaa atactagaac cttacgcggc ttacacggtt ggcgaaacaa
    15481 tggatattac tattgacgct gctaaagatt atttagatgg tgaattaacg caagtgtttg
    15541 cctttgatca agtgcaagtc gattactttt tattaccaac atttaaacgg aaatataaac
    15601 cgcatcggat ggttaacgcc ctaaagaaat ggcaacttaa aataccttgg aatacagtct
    15661 tttatgaaaa tcatgacttg ccacgtagtg tgagtcgctt tggtagtaaa aagtattata
    15721 aggaaagtgt tacggcactt ttagcgtcag tcttattctt acgcggaaca ccgtatattt
    15781 atcaaggaca agaaattgga atgacaaatg ttgattttgg cggcattgaa aatattgaag
    15841 atgtaagttc gcgtaatgtt tataacttac tccgtaaatt taaagtaccg cgtaaacttg
    15901 cgtggcgctt tgtgaataac tttacgcgcg atcatggccg cagtccggta cagtggaatg
    15961 ataaaaagca cgctggtttt actactggta aaccgtggtt aatggtgaat ccaaactata
    16021 cagaaattaa tgttgaagct caggaaatag atcaagactc agtattacat cattatcgaa
    16081 aaatggcgac tttacggcaa aaaacaaaag cttttaaaaa tggcgaccta gaatttaaga
    16141 aaacacatcg tgacatttta gcgtttacgc gcacagatga tttaaacgaa tatttagtcg
    16201 tcattaattt agcaagtaaa gcacgcactc ataagttaaa tcttgatggt gaagtacttt
    16261 attccaacta tcaagagttt agtgataagg ctaaaaagtt gcgcccattt gaagtgcaaa
    16321 ttataaaacg cttagattaa gactcttaac tagcccttaa cctatagcat ttatgctata
    16381 attagcgctg ttaagggagg ctaatcatga aacgtaaaga ttatattaat tgggatgaat
    16441 attttatggg aattgcgatt ctttcttcga aaagaagtaa agacccaagt acccaagttg
    16501 gtgcatgtat tgctgacgat gaaaataaag tcgtcagttt aggttataac gggatgcctc
    16561 gcggaattga tgatgatgta attccgtggg aaaaaggtga agatatcaat aataaatatt
    16621 tttacgtatg tcacgctgaa cttaatgcca ttttaaatac aagaagtgga gcctcactta
    16681 aaggatgtcg cttgtatgta acacttttcc catgtaacga atgcgctaaa gcttgcattc
    16741 aagtcgggat taaagaaata gtgtatcttg atgacaagta tcatgaacaa gtgaatatgc
    16801 gcgctagtcg ccatatgctt aaattagcag gagtgaaatt aacaccattt aaaggtaaag
    16861 tgttagaccc caaatttaat gaataaatat gccttagaaa taatgaactt aaattactct
    16921 tacgacaaaa atgtcgaagt tttaaacggc gtcactttta atgttaaagc gggcacttat
    16981 gttagtgtta tcggtcataa cggcagtggg aaatcaacat tagcgaaact tattgccggt
    17041 attttagatg tcggcaaagg gagtgtcatt aaagtatttg gtaagccctt atctagttat
    17101 gatgatgaac gaccagatat cgggattgtt tttcaaaacc ccgataacca atttattggt
    17161 gcaacggtgc gtgatgatat tgcttttggt ctcgaaaata aaagagtagc ccatgccgag
    17221 atggatgaca taattaatga atacgccgct aaagtaaaaa tgagtgagtt cttagataaa
    17281 gaaccaagca acttaagtgg tggacaaaag caacgcgtgg cgattgctgg tgtgttagcg
    17341 atgaaaccac gcattttact actagatgaa gcgaccgcga tgcttgatcc acgtgggaaa
    17401 cgcgagatta aagaaattgt gacagagtta agaaaagaag taccagactt aacaatcatc
    17461 agtattacgc atgacttaga agaaacatta catagtgatg aaattattgt gatgaatgaa
    17521 ggcaaaattg tgctgcaagg tacaccggaa gacatttata agaatcacga aacattaacg
    17581 agtattcgcc ttgatttacc ctttattttc cggattaaac ttgaacttga aaaacaagga
    17641 attaaagtta agagtaaaga tttagaaagt cttgcggagg aattatggcc ataaagtttc
    17701 accatattta ttttagttat gcgccaaagt ctccttttca atatgatgca ttgcatgata
    17761 ttaatttaga aattaagcaa aatagtttta cagcggttat tggtcacact ggtagcggaa
    17821 aatcaacgct tgttcaacat attaatgcac ttttagtccc aacttctggt gccgttaaag
    17881 ttgaagattt tcttattttg ccttgtaaaa agaaaaagta cttacgacaa ttaaaccgta
    17941 agcttaaaag taaaaaagta agtgccgaac aaaagcaaag actagcgttt ttaaaaagta
    18001 ttgttgaaag tggcgctgct tataaagtta aagaattacg aaaaaaagtt ggcgttgtct
    18061 ttcagtttcc tgaatatcaa ctatttgaag agaccgtcat taaagatgtt gcttttggcc
    18121 cgaaaaattt tggggtgagt aatgaagaag cactagcgtt agcgaaaaac gctttaacat
    18181 tagtcgggat tgatgaatcc ttttatagtc gttccccatt tgaactttcc ggtggtgaaa
    18241 aacgccgggt ggcaattgct ggtattttag cgcttaagcc tgaaatttta gtgcttgatg
    18301 aaccaactgc gggtctagat cctttaagcg cccatgaaat gatggaagtt ttcgctaata
    18361 ttcacagagc ggggacaaca atcgtgttag tcacacatga tatggattta gtgctgcgtt
    18421 acgctagtga tgtggtggtg atgaaagacg ggaagattgt cgaagaaaca acaccacaaa
    18481 aacttttcgc tgatgtgaaa gaagaatata gtttagaagt accagcattc tatgctttta
    18541 tgcaagcgtt agtgcagcgc ggctgtccac ttgatattac taaaataaat actgtaagtg
    18601 atttagcaaa aatggtgaaa aaccttaagg agcaagcaaa gtgaaaaata tctccttagg
    18661 gcgttatatg cctgctgatt catttatgca taaagctgat ccacggatta aattttttgg
    18721 tttagtcgta tttatggttt taatcttcct aaagtttgct aatcctgcca tggacttttt
    18781 gatgtacggg atttacgctg ttgttgtttt tattttaatg aaagtaacaa aattaaagat
    18841 tttttcactc tttaaacaac taaaaagctt gtggtttatg atgattttct tactaataat
    18901 taatatattt acatttagaa atagtgaagg taatatctta ttcatgattg gtaactttgc
    18961 gattaaagat gcagcgcttt atcaaacact atacattttc attcgccttg tgttaatgat
    19021 tgctttaaca tcggttttaa ccgcaagtac gagaccgcta gacttaactt atgcgattga
    19081 gtggtatatg agtccgctta aagttattcg ttttccagtt catgaaatag cgatgacact
    19141 gtcaattgca ctccggttta ttccgacttt acttgatgaa acgggacgaa ttatgcgagc
    19201 gcaagaaagt cgtggtgttg attttaaagg cgggaaggta aaagaaaaat tacgcggtgt
    19261 tgtcgcactt attgttccgc tttttgttag tgccctccaa cgcagcgatg agctcgctaa
    19321 tgcgatgatt gcgcgcgggt atgacccaca ggctaaaaga acgcgttatc ggattttacg
    19381 gtttacttgg cgtgatttaa ttgttttatt actagcggtt agttttttaa cgcttgttat
    19441 tttactacgt gtctatcata ttgatttagc aaatatgctc tatgttaata tccaaaattt
    19501 actatctctt tgggggctta agtaatgcgc tacgtcgcaa tagtaaagta cttaggcaca
    19561 aattactttg ggtggcaaag gcaacccttt gaaattagtg tccaacaagt aatagaagat
    19621 aagttatcaa caatttttaa tacggaaatt gtaatttatg cttcaggtag aacggacgct
    19681 agagttcatg cttacgggca agtgtttcat tttgacgctc cgccttttcc tgttagtaaa
    19741 ttaaagtatg ccttaaataa aatgctacct ggtgatattg aaattgttaa attacaaaaa
    19801 gcaaaagatg actttcatgc gcggtatgcc gctaaaagta agacatatga atatctaatt
    19861 tacttaggtg caaaagatcc gtttttattt aatagagtgc ttttttatcc ttttgcatta
    19921 gatgagaaaa agttacggaa agacgctaaa ttatttgtcg gtacacataa cttccaaaac
    19981 ttcacaagta agaaagaaga tgaggctaat tttgtccgta atattagtgc agtgaaaata
    20041 caaaagagcg gcaaaatgct aaagattgtg tttattggtg aaggatttat gcgtggccaa
    20101 attaggatga tggttggcgc attactcgcg caaaatgcgg gtaaagaaga tgcaaacttt
    20161 attaaagaca acttaaattc aaaagaacga aaaatcatta gtcataaagt aattggtgat
    20221 gggctttatt tacgcaaagt aaagtattag gcttataata aaatagtagt taaaggagca
    20281 aagttatggg aagagcattt caagttagag cggcaagtat ggcaagtacc gcagcgaaaa
    20341 agagtgcact taatatgcgg gcctcaaaag aaatttttat ggtggcaaaa agcggaggca
    20401 ctgatcctaa ttccaactta gccttacgcg cagcgcttga taaatatcga gcaatgggtg
    20461 cgacaaaaga tgtcatggaa cgggcgatta aaaaggcgga aggagatgac gcttttgctt
    20521 atattccagg gcgttacgaa gtgatgggcc caggtggcag tcaacttgta attgatactt
    20581 taactgacaa tgttaatcgc gcgattagtg atattaaagc tgtgcttaaa aagacggact
    20641 cgatcttaca aacagttgct tataacttta ccgaaacagg tatcttttta ttcgcaggca
    20701 ataatcttga agaaataaca gagacattaa ttcttaatga tgttgatgta tgggatatta
    20761 atgaagaaga tgatgtgatt gaagttcttg ttaatccgac ttcatacggt aaaacacgcg
    20821 atacattata ttctttaggg attaaggaat ttaacattag tgaaattcaa atgattccta
    20881 acgataaagt aacactaaat gcagaagaca ctgaagtatt taaacgcatt attgatatgc
    20941 tagaagatgt ccaagatgtc caagaagtgt atcataatgt taatgtgccc actgaagagt
    21001 gatataatat tagtggcaaa aatataagga ggaactttat atgcgtagaa atgcaaatcc
    21061 aatattccgt aatatgaata atgatatgaa tcaagcaatg acaagtagtg tgccagttga
    21121 tgtgacagtt agcacagcat catggagcgg cattgccctt aaaacattat tactcgtact
    21181 cattatgatt gctgcgggac ttagtacatg gtttttacca ttccctgtcg tcgtcggttt
    21241 tttaatcttt ggtggtattg cttcattctt cttcgtgttc ttcgcaatgc gaagtgtgaa
    21301 aactgctcct gttttaagtg tgttatatgc aataactcag gggataatgt atggcgcact
    21361 gacatttatt gttgaaacct acttccctgg tgttggtgta atggcacttg tggcgacttt
    21421 tggcattgtt ggtgtgatgg ctttcttaca tagtattaaa gctgtacgcg ctacaccagg
    21481 cttaattcgt tttgtttttg cagccttatt aatggtaatg attagtagtt tagtactttt
    21541 cattattagc cgtgttgccc aagatttata tttaagttat gttgataatt acccgctgat
    21601 ggtcattgtc agtgcattct tagtcgtgct tggggcgatt atgctcattc ttgactttaa
    21661 taatgctgaa acaatcgtcc aaagcggagc gccaaaaatt tatgaatggc aagtaagttt
    21721 agggttctta gttacccttg tgtggattta tttacaagtc attcgctttc ttcttgttct
    21781 cgctcggaat cgagaataag gaatatatgt tagcaaaaag acggccacta catgtggtcg
    21841 ttttttcttt attgtcgcgc tttactttgt aaagaaacct ttacgaattg cttatactgt
    21901 taaaattcac ataaatgaag ttataatgct tataggtgat taatatgata aaagtaaaaa
    21961 acttacacaa aacatataaa aaaggtgtta aggctgtaaa tggtcttaat tttaaagtta
    22021 aagctggaga aatttttggc ttattaggtc ctaatggtag cggtaaaacg acaacgatca
    22081 acgctattct tgatttagtg gattatgaaa caggtgaaat tattttattt gataataaat
    22141 ataagactaa agttgaagcc aaaaagcata tcggacttat tacccaagaa gttggtgtct
    22201 ttaatgaatt aacagttaga gaaaatattg attatttctg cggactttat ataaatgatg
    22261 aaaaactccg tgcacaatat gttgaagaag cgatggactt tgtgcaaatt aaagattttg
    22321 caaagtttaa accaaagaaa ctttcaggcg gattactccg gagattacat atagcgtgcg
    22381 gcattgcgca taaaccaacg cttattttct ttgatgaacc aaccgttggg attgacccac
    22441 agagtcgtaa tcacatctta gaacaaatta aagtattaaa taaaaacggg gcgacaattg
    22501 tttatacttc gcattacatg gaagaaattg aaatgctatg tgactatatt ttaattatgg
    22561 acaaaggcca agttattgcc gaaggaaatt atgatgcctt aattgatcaa atcgatttag
    22621 cggaatacat tgaaattgaa tataaaggcg aaatgccgct aaaagttgtg acagaaataa
    22681 atgaattacc aggtattctt gaagtgatcc acactaagga gaagttagta attaagacga
    22741 gtgaagacga taaagcattt attaaaatta ttcatatttt agaaaataac aatgttgatt
    22801 attcgcgggc taatgcgaat aaaccaagtt taaatgatgt atttttggag ttaactggta
    22861 aagagttacg ggactaagat gaaaagttta tttattttaa atttaaagaa attacttaaa
    22921 aataaagaaa ttgttttttg ggcgttatta tttccaatta tattaagtac tatttttcat
    22981 attgtgtttg atggtattcg caatcgtgat aactttgaaa cacttaaagt aaattatgtt
    23041 tccacaaact ttactgccga ggacaaagcg tttttagttg aagaaatgct cactaacaaa
    23101 gctgaataca ttaaaaacga aggcacagat aaagcacaaa cagtaaagat ctttgcactt
    23161 agcgaaaaaa ctaaggctga agccgaaaaa gaatttaaaa aagataagac catttatttt
    23221 tataaggaag atggggcatt taaaattaaa gcatcttcac aaaatataag taccgcgata
    23281 ttacaaacat ttattgatga ctatcaaaac tttattaagg ttacgacact cttagtcgca
    23341 gaaagtaatg gtacattatc atttcaagac gccatgaata aacatgttgc aagctatgct
    23401 tatacatatg actttgattc tggtgataaa aaaatagatt atgttaataa ctatcactat
    23461 acagtacttg cgatggcgat aatttatggg gcatttcttg cgacagaaca agctaaaatc
    23521 tttcaaccaa atagttttgc ttttgctaaa cgcgttacag ttagtggcgc tagtcgcgct
    23581 aaattattag tgagtgcctt acttagttca ctcgttgtgc aaacagcaat tattgcgctt
    23641 tacattgcaa tattagtttt catttttaaa gtgacatttg ctaatattgg tttagcgctt
    23701 ttagctgcat taattggtgt tattttagtg aatataaatg gctttttcat tgggattgtc
    23761 tttaataaac ttagtcaaaa tgcagtgatt gctatcatta ttttagcggg tactatcggc
    23821 gggttcctct cagggatgat gatatcatca atgaagtttt ttgttggcac taaaatgccg
    23881 cttttgtctt atataaatat caacgcatta attagtgatt catttgttag tcttgatttt
    23941 ggtaatatga ctcgttactg gaataatatg ctagcgatga ccgttatcgg tgtcattctc
    24001 tttggtgtaa cgcttattaa atttaatcgg gagagcaata aataagatgg ggtttaaatt
    24061 atttttcaaa attgttaaag caaacttcgt ttcatttctc gcttatgttt taattttagt
    24121 tgttagtggt cttatgtttg ggttaagcga tagtggcgcc gctaatgaag actttaaaat
    24181 tttagcgcca gtctactacg caaatattac aaatgaaaca gtcgatgact taccaagtga
    24241 taacacttta attttcgatt atttaatgga caataattta attagtgaag aaagtgatga
    24301 tccgttttcc caaggctttt atacatacac tttaaacttt gtgaatgtaa agaaaaatat
    24361 taagccaagt gaagtaaatg acgctattta tgccggagtt gtgcgtggcg cactcattat
    24421 tccgaaggat ttatcaactt taacgcggga aaatgtatta tttatttttc atacgaattc
    24481 gcaagattta gcgacaataa ccccaacaaa agccgccctt aataagttta ttaatactta
    24541 ccacgactta aaagaagtta gtgctacagc cgggaataac ttatccgcta ctgagttaac
    24601 aaacaaaaca ttaattgcgc ttgaaaaaga aacggagata gtttatgaaa atccaaatcg
    24661 cacaaaactg tcgatgttaa gccatttctt ttcattttca acttacatta tgtcagcagt
    24721 tattatcttg cttgttagta ttgtaatgta tgaaattaaa cgccgtgaaa tattaatgcg
    24781 tatttcgata acgccctaca gcatcccgcg cttaatcgtg ggccttatct ttggagcaat
    24841 aaccctttcg cttctgttag taggcctagt ttatgtctta gcaattattt cttatccaaa
    24901 aattgcttta agcataactg gattatactt aacacttaat attatgctat ttactttacc
    24961 acttacggca ctagcgatgg tcattggcct tgtaattagt aaagctgaaa tcgtctccat
    25021 cattagtaca gtctttgctt tagcgcaagc ctttttctct ggagcttttt taccaacatt
    25081 cctcttacct aagtcgatta ttaatgttgg gaaagtcttt cccgctagtc atacagtgac
    25141 aattaataac ttgcttgccg aaggattaaa tacaaacgga ccaagcatct tattaaatat
    25201 cggtatttta ctagcctact ttgcagtatt ccttacaatt gccatcattc ttagcaagaa
    25261 aattacgaaa cgcgaaggtt aatttcatct ttatgtcggc ttatcaatta tattgataaa
    25321 taaaataacg aattaagttg acaatatatt tataatatat tattattaat cacggcatta
    25381 tctgcctttt gtcgtcccgg taagacgaaa attggtaaag gagaaataca aatatgctac
    25441 gtcaaacaac acatgcgaat gctgcaaccg ttgaacgtaa atggtatgtc gttgacgcta
    25501 ctgatcttcc actcggtcgg ttagcgagcg aaatcgccat gacgctcatg ggcaaaaaca
    25561 aaccagaata taccccacat gttgattgtg gagattatgt tatcgttgtt aacgctgaca
    25621 aggttagttt aagcggcaat aagctcaaaa caaagaaata ttacaacacc agccaacgtc
    25681 ctggcggttt acgcgtgcgg acagcaggtg aaatgctcgc aaattatcct gtcgaaatga
    25741 tggaaagagc agtgtggggc atggttccaa aaggtcgtct tggccgtcaa attattaaga
    25801 aattatttgt ctatgctgga ccagaacaca aacatagtgc acaacaacca gtggaactac
    25861 ccttacgtta tattggagga agtaaataat ggcaaagaga aaagcagaaa cttacttagg
    25921 tactggccgt cgtaaatcaa gcgtcgcacg cattattttg aaacctggta aaggttcaat
    25981 tattgttaat gatcaagatg ttaatgatta catgccttat caaaccttag ttatggactt
    26041 aaaacaacca ttaactttac ttgatgttgc cgacaaatat gatgtcatcg ctaacgttca
    26101 tggtggtggc tttaccggtc aagcaggggc aatccgttta ggaattgcgc gtgctttact
    26161 tgaagctggc cttgatcgcg gtacacttaa acgcgcggga atgttaaccc gtgatgctcg
    26221 tgccaaagaa cggaagaaat acggtcttaa aggcgctcgt cgtgcaccac aattctccaa
    26281 acgttaatat aactacacga gaacaaacga aagtctggtt caaaatgcca gactttttct
    26341 tatattaaaa acttttaatg ttaaaaaagt tgcaatatga ttaatgttgt tatattatat
    26401 attagcgtcc gcagatgggc ccttagctca gttggttaga gcgcacccct gataagggtg
    26461 aggtcgatag ttcaaatcta ttagggccca ccatttgcaa gtaaagtcac tactttgtag
    26521 tgattttttt gtaaagaata agtaaacttc ctctatttac catacataaa tgttaaaata
    26581 cgatggtgag gtgaaaatat gaaacaagaa ctaattaaaa acaattatta cgaagctgtt
    26641 aataaagaat ggattgatgc cgcagtaatt cctggtgatc aaccaatgat gtcagcattt
    26701 ttagaactac atttagatat tgaaaagtta ttaatggatt tagcgaaaaa atgggataaa
    26761 gaccaaacag gattaaataa tcatctgcaa gaattcgtta agctttacaa aatgacgaat
    26821 gattttgcta caagagaaaa gctcggtgct gaaccactta aaccagtaat taagaaaatc
    26881 ttaaatttaa agtcacttaa agacttagaa aaaaatatga aagattttgt gttagatgga
    26941 gtggatttac catttagctt tatggtgttc caagatttta aagattcaaa taacgaggtg
    27001 ctatatttta ccgctgcgaa tttattctta ccagatactt catattatga aaacgaagaa
    27061 acgaaacaac aacttattgg gttatttaca atgacgacaa tgcaagtgct aagtttatat
    27121 ggtttagaag aagaagcggc acaagactta ttaaataaag cgttagcgtt tgacgcgatg
    27181 ctagtgccag tgacgatgtc gagtgttgaa agagccgaca tgattaaaat gtataaccca
    27241 ttattaaaag atgaatttgc cagtaaaaca aaaacattta atttactcaa agttgcggaa
    27301 gcattagtcg ggcaagatgt tgataaagta gtagcaatga atttagactt tattgataag
    27361 tttgaagaaa ttgttaatga cgctaatttt gaactaatta aagcgtggat ggttgtctct
    27421 aacatcctta cattcagcgc tgaattaacg gaagaattac gggttgctgg tggtgctttt
    27481 ggccgcgctt tacaaggtat tgcggaagca caatcacaag aaaaagcagc gttttatcaa
    27541 gcttatgacc gtctttcaca agctgttggg ctctattacg gcttaactta ctttgggcca
    27601 gaagctaaaa aagatgttga agatatggtt aatactatta ttggtgttta taaagaccgg
    27661 attaataaaa acacatggct taacgctgaa acaaaagcaa aagcaattct taaacttaac
    27721 accatgaaag tacatattgg ttatccgcgt gaattaccaa aatattatga taaatacaaa
    27781 gttggcagtt acgctgaagg gtcaaattta gtgaaagaaa aacttaaata tagccgttta
    27841 gtgcaaaaag aaaactttaa gaaatataat aaaaaaccaa atcgtaattt atgggcaatg
    27901 ccagcttcaa tggttaatgc ttactatcaa ccaatgaaca atcaaatcgt tttccctgct
    27961 gcgattttac aagaaccata ttatagttta agtcaaggtc gtagcgctaa ttacggcgga
    28021 attggtgcag ttattgccca tgaaatctcg cacgcctttg ataataatgg cgcaaagttt
    28081 gatgaatacg gctcacttaa taactggtgg acagatgaag atcttaaaac atttgaagaa
    28141 aaagcacaaa aaatgattgc attatttgat ggtgttgaat caggatacgg gccgtgtaat
    28201 ggtactttaa ccgtttccga aaacatcgct gatagtggcg gaatccgctg tgccttagaa
    28261 gcaagcaaaa aagaaaaaga tcataattat gaagaattct ttaaaaattg ggcgcgtgtc
    28321 tggcggcaaa aatctagtcc agaatttatg caattactct taactgttga tgtgcatagt
    28381 ccatcaaact tacgtgcaaa tatgcaactt aaaaacctcg ttgaattcca agagttttat
    28441 aatttaacgg aacaagacga aatgtattta gcaaaagagc aaatggttga aatttggtaa
    28501 acaatcaaat cttgtaaact aagagggctt gtccctcttt tttaattgct atttataaat
    28561 gattttgcta ttatattaca aggtcgtccc ttccggacac ttagctcaga tgggagagcg
    28621 tttccttcac acggaagagg tcgggggttc gatcccctca gtgtccacca tttatttaac
    28681 gccgacgtgg ctcaattggc agagcaactg atttgtaatc agtaggttgt aggttcgagt
    28741 cctatcgtcg gcaccattac ttgcaaacgt ggcggaactg gcagacgcgc tagacttagg
    28801 atctagtggg gaaacccatg caggttcaat tcctgtcgtt tgcacca
//
LOCUS       DAOSTA010000011        28714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_1803_length_28714_cov_11.968596, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000011 DAOSTA010000000
VERSION     DAOSTA010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 28714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28714
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1803_length_28714_cov_11.968596"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..440)
                     /locus_tag="PLU08_01960"
     CDS             complement(150..440)
                     /locus_tag="PLU08_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70505.1"
                     /translation="MHDRKTAFNFATWLFIYDFGKCDIRNPYPFLALLFKKMELDKDI
                     QDNLNNRFDTPYDRFLDLYVDVLISSKIINANEADYLIPDKDTMLLDELEKL"
     gene            complement(741..1514)
                     /gene="trmD"
                     /locus_tag="PLU08_01965"
     CDS             complement(741..1514)
                     /gene="trmD"
                     /locus_tag="PLU08_01965"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOQ70506.1"
                     /translation="MKITILTLFPEMFTNFLETSIIKKARLQGHVTFDIVNIRDFADN
                     KWNRVDTPPVGGGAGMILQAEPIVKALESVDSGHKLLLSPRGNVLTNMKATALSQKEH
                     LIFICGHYEGIDERVNNFIDEEISIGDYILTGGELGAMVISDAAIRLLDEVISTESLD
                     IESFDYHLLEYPQYTEPYDFRGYTVPTILYSGNHAAIRKWRLKESLRITRERRPDLFK
                     KITKDNEIKKLLKEIDDDVIGEWEKDAIEKGSRFTKNKT"
     gene            complement(1511..2026)
                     /gene="rimM"
                     /locus_tag="PLU08_01970"
     CDS             complement(1511..2026)
                     /gene="rimM"
                     /locus_tag="PLU08_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOQ70507.1"
                     /translation="MNKDYLKIGYIVETHALKGALKIKPTTSFAEERFEVGNKVLVKN
                     NTTSKTETLTIQSVREQKGLLIVTFAEIDNINIAENYLKGFLFIAKKDITLEEGYFFF
                     DDLLGMKVVLEDETLVGEVIEILEYAAYHTLRVKREDGSNILIPYIEEFIVSTDLASK
                     TIVFRPIEGML"
     gene            complement(2067..2321)
                     /locus_tag="PLU08_01975"
     CDS             complement(2067..2321)
                     /locus_tag="PLU08_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOQ70508.1"
                     /translation="MRDYEALVRTIITPLVKNEQALLIRQTNEGHRIIINIFAESDDI
                     AVLIGRRGVVANALREVVSVAGKLDEEHVFLRFEALDENK"
     gene            complement(2324..2599)
                     /gene="rpsP"
                     /locus_tag="PLU08_01980"
     CDS             complement(2324..2599)
                     /gene="rpsP"
                     /locus_tag="PLU08_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012094939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOQ70509.1"
                     /translation="MAVKIRLTRVGRTHDPHYRIVAADSRYARDGRIIEQIGHYDPKK
                     DLKEAVIDRELALKWLGNGAQPSATVKALFRAQGINEEFAKSKKSGK"
     gene            2753..4159
                     /locus_tag="PLU08_01985"
     CDS             2753..4159
                     /locus_tag="PLU08_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOQ70510.1"
                     /translation="MKNLLNKLFKFEERKANLTSEILGGLVTFLAMIYILPTNAGILS
                     DMGMDKSGVFVATAIVSAIVSLVMGLYGNFPIVLSAGMGVNAYLAYVVYGALGSWQAA
                     LLVMFFAGIIFAVISLSPLRKMMIDAIPNDIKYIISAGLGAFIAFVGFKSSGFIVDSP
                     ATLVSLGNLRDPMVLLSLFGVLLVVIFMFSSNKRLNQLAIPIAMIIVVVVSVAVWNIF
                     ALPEATDAGRPIAFHFTNIKDMFSNPTSWGGQGFEKVAFKIFDKETWGTALSNPSTYA
                     FIFSLVLVNLFDTTATILAVGRGAGIIDADGKMVGGHKVVAADSFGAVICAPLGTSTV
                     TSFSESGIAVEMGAKTGLAAVVAGFMFLLSAFIYPIFYIFTEPAVTSMALISVGGLIF
                     VGNLKDVNFDDRIIGFTAFFTIMLILLTYSISDGLGLGLIMYVIMMFVSKRGKEVKIP
                     MYVVAGVFVIYFILKALV"
     gene            4341..4688
                     /gene="rplS"
                     /locus_tag="PLU08_01990"
     CDS             4341..4688
                     /gene="rplS"
                     /locus_tag="PLU08_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOQ70511.1"
                     /translation="MNKNLVNEINASQLRTDLPEFSSGDTVKVAVKIREGDKERIQFF
                     EGVVIAMQGAGVNHTFTVRKMSSGIGVERVFFTHSPVIDSVEVLRRGKVRRNKIYYIR
                     KLSGRKARIKEKK"
     gene            4811..5542
                     /gene="lepB"
                     /locus_tag="PLU08_01995"
     CDS             4811..5542
                     /gene="lepB"
                     /locus_tag="PLU08_01995"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOQ70512.1"
                     /translation="MVIEEVLINEEISEAEINRKNKPRLRKALSVTWTILLVTAIVVS
                     LFVTYVDLTYTPVYVDGPSMQPTLNDFHHRYYEFGLMDERPKARNNIKRGDIVVFRRN
                     GINIIKRVIALPLETIQIIDNPTGDEVFITLPEATEKFKLDEPYLSTSNRYTTATYLN
                     DSSPKYENGLKEPLTLEEKTYYLMGDNRANSSDSRAIGPVHFDNISGKLVVIQGYTEN
                     VEVSESGKVKFIGQHYYVLWKWRYY"
     gene            5542..6369
                     /gene="ylqF"
                     /locus_tag="PLU08_02000"
     CDS             5542..6369
                     /gene="ylqF"
                     /locus_tag="PLU08_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOQ70513.1"
                     /translation="MSKQIGWFPGHMQKATRKLIETLKMVDIIVELGDARIPHSSRAT
                     YLTAIIERKPNLYVLTKADLADPKRVSKHTEKLNVLAGNLNERKFVRQILAKIKEIGQ
                     PIIERDVRRGLKPRPLAVMIVGIPNVGKSTLINKLAGRKAASVQNKPGHTRANQYIRV
                     SEEIALIDTPGILSPNYDEEDTSMKLALIGTIRQEILPTHTMAEYLVNYLKTYYFEEM
                     VAFYDIDIKEDSNYQTVISAIATRRGIIGEDFDVSDRTEILILNEFKNGLIGRISLE"
     gene            complement(6440..7276)
                     /locus_tag="PLU08_02005"
     CDS             complement(6440..7276)
                     /locus_tag="PLU08_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70514.1"
                     /translation="MKKRKFLLIPLLLGLLLTGCFGGDGPEDIIDGDDKSSQKTTNDV
                     AEEDILENMDYEKREGWPTQKLATFLSSERVNETVPALTNIATTYYHENNDANNKYFA
                     IVVPGANREADYKAILEGAGYTYSKLSWTSIAVNTEDTINIAYQYMQDHPVLDYAMYI
                     VISALKADSFRFDKLESDIGWPANQLQTFLSKYGITERVPSLTGGNYFAELTERNEFI
                     VCAPGVNFVDSYFGSLENEGYTVNGSNAVSTSGKVRINVTANDYPFFFFDATKIVIKP
                     VV"
     gene            7461..8087
                     /locus_tag="PLU08_02010"
     CDS             7461..8087
                     /locus_tag="PLU08_02010"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOQ70515.1"
                     /translation="MTYFEFDNDVRKHGHLYLVGVDEAGRGPLAGPVFAAAVILPPFF
                     NDERIKDSKQLTATELLELSDLIKEKALDYQIVHVDAPVIDQVNIYQATILAMQKAVT
                     GLSHEFHFVLTDAMPLSNLEVGYEAIIQGDATSLSVAAASILAKVARDAYMNKLAKLY
                     PQYDFENNKGYPTRKHLQALDTYGPIKDVHRYSYKPVQDALNKQIKLF"
     gene            8137..8919
                     /gene="dprA"
                     /locus_tag="PLU08_02015"
     CDS             8137..8919
                     /gene="dprA"
                     /locus_tag="PLU08_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOQ70516.1"
                     /translation="MNKKARDVLLYFASKYEGNYHAIINALRSKEPVDDALFNKYKED
                     WEKVDTITIIDEDYPALFKTVNNPPIVLFLKGNKELLFNLEKSIAVIGAREYSEYGKN
                     MTYEIVEGLVKEKFTIVSGLARGIDGFSHDACLRAGGNTIAVLGSGINYPYPKSNKKL
                     YDEVSKHGLLITEYPNDTKPEPDYFKFRNRLVAALTKAVLIIEAKYRSGTIITVGYAL
                     EKGSDVFCVPERAGRNSGCNRLIKDGAYLVESASDIIKLWPN"
     gene            9028..10983
                     /gene="topA"
                     /locus_tag="PLU08_02020"
     CDS             9028..10983
                     /gene="topA"
                     /locus_tag="PLU08_02020"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOQ70517.1"
                     /translation="MKLVIVESPSKIKTIERYLSDEYKVLASYGHIVDLSTRGRKNLG
                     INLETFEGDYVLDKSRQKVADTLKKAKNESSEVYLATDPDREGEAIAVHIAKLLNLDL
                     NTTKRLEFHEITRDGITNALAAPRLLNEDLFNSQEARRMLDRIVGFELSSLLNQKINV
                     KSAGRVQSAVLKLIVDKENEITAFVPEEYWTLKIDTKYDDALFSVNFNKSYAGDTKIV
                     NEAMKNDIVSKLAPKIVVTDVKSEIKHTNPKPPFTTSTLQQEASSKFKYSASRTSRII
                     QKLYEGIQINGIETGLVTYIRTDSTRLAPSFVANASQRIVKEYGAEYLGRTQARGKTL
                     QNVQDAHEAIRPTNLNFTPDSIKDSLTREEYNLYSLIYTRALASLMVGSKEQVTRYTF
                     NSNGTIFNATSTILIFPGFRKIYGKDEETVDDSALPQMHVGSELPIIDVITEQKFTEP
                     PARYSEGRLIKTMEELGIGRPSTYAPTIQILQKRKYVSSLKGTLKPTDIGFRQIDALS
                     EFFPSIISSDYTASMENELDKIANGEGDKVEFLSDFYSDFSQKIDDARKGMKKLPPEK
                     VGRDCPRCGSNLVYREGPYGRFIGCETFPKCKYTEFIVEYTGENCPKCNEPLTYRYDS
                     RNRKYIRCSNYKSCDYSATIKSTRRKK"
     gene            11029..12321
                     /gene="trmFO"
                     /locus_tag="PLU08_02025"
     CDS             11029..12321
                     /gene="trmFO"
                     /locus_tag="PLU08_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOQ70518.1"
                     /translation="MEQVVIIGGGLAGVEAAFFLAEKGIKVKLYEMRPGVMTPAHHSA
                     KLGELVCSNSLKSKELTNACGLLKKEMEIYNSLMTSVAARVSVPGGNALCVDRELYSE
                     EVTRIITTHPLIKVVNEEVVELPSHVPVIIASGPLTSKGLENTISKLIDTAFLHFFDA
                     SAPIIEKDSINMDIAYYKSRYDQGDDAYINCPFTKEEYYRFYDELINAKLAPIHEMDT
                     KYFDACMPLEVIAKRGPKTLRFGPLKPRGLGKDEHHKPYAVLQLRQDNVIGSLYNLVG
                     FQTNLTYAEQRRVFRLIPGLENAEFVRYGLMHRNTFVNAPRVLNLDKSLKVNNDIYIA
                     GQLSGVEGYVESAASGLYVALNVYAKIKGVKLEYPCETMLGSLMHYLNNANPENFAPM
                     NANFGIVRGATKQNRMEIAEKSLKITQKYKEYIESLNE"
     gene            12314..13228
                     /locus_tag="PLU08_02030"
     CDS             12314..13228
                     /locus_tag="PLU08_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOQ70519.1"
                     /translation="MNRPLQEFQDYLRYTRRFSENTVIAYISDIETFYLFLANQNMRD
                     DEVDKPLIRLYLKDQIDKYVSRRSIQRRLASLRHYYSFLQDKRYVKDNPFLTVQAPKA
                     TKALPRVLYEEEIRLILLANEKRTDYLRDRDQLIIELMFATGLRASEVINLTLQSFNL
                     NERFLMVLGKGKKERMVPFTDRVQVLLRNYLNGLRKELLAKRKSSQPTNILFLNAQGN
                     KLTIQGLRYILLSIEKKTGEYVNLHPHLLRHSFATYLLEKGANLRVIQELLGHESINT
                     TQIYTHVSEEKITKEYYTAHPRAKKKID"
     gene            13400..14539
                     /gene="rpsB"
                     /locus_tag="PLU08_02035"
     CDS             13400..14539
                     /gene="rpsB"
                     /locus_tag="PLU08_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOQ70520.1"
                     /translation="MSEEEKVVLTEEEVTPAMENVLPDENLPVITMKKLLEAGAHFGH
                     QTKFWNPKMKPYIYGSRNNVHIIDLEKTIVKIEEAYAALREIVTRGGKVLFVGTRKQH
                     AEHVKSEAERSGSFYMNNRWLGGTLTNFRTISTRVKYLRDLEMQEAEGAFDRLPKKEA
                     ILIRKEMKRLNDNFEGIKEMRKVPEAVFVVSTIDEANAIREARNLGIPVFALVDTNCN
                     PDVVDYIIPSNDDATKTVQLMLTLFADAIVEAKGGIPVLAYTRDAEPETETLEEALKH
                     NEPRARRTDSEERRQVRKAQTQHSDKPTRVRKTEAKEEGEAAPEVAEKPAAAAKPKKE
                     KEVKEPKAEKAEEPKVKAEPKAKAPKAPKVEEVKTEEVKTEKEGE"
     gene            14543..15403
                     /gene="tsf"
                     /locus_tag="PLU08_02040"
     CDS             14543..15403
                     /gene="tsf"
                     /locus_tag="PLU08_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013489060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOQ70521.1"
                     /translation="MASLIDKIKELRELTGAGMMDCKKALVENGEDVDKAKDWLREQG
                     ITTAAKKSGRIAAEGLALAKIEDGYAAIVEVNCETDFVSRGDDFKALVNTVLDLLLKH
                     KPANKEAADAIINPLLVEATVKIGEKLSFRRFELVKVEDRGATYIHMGGKIATVVLLD
                     KKNDELAHGLTMHIAANSPSYITTDDITEEDYNHERGIQLEITKNDPRLADKPEKMLK
                     NIVEGKIRKYFSDLVLAEQTYLMDDTKKVGQALKDLDNKVLVMFRYQVGEGIEKRQED
                     FAAEVFGQIK"
     gene            15454..16173
                     /gene="pyrH"
                     /locus_tag="PLU08_02045"
     CDS             15454..16173
                     /gene="pyrH"
                     /locus_tag="PLU08_02045"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOQ70522.1"
                     /translation="MVYKRVLLKLSGEALAGDKKSGIYDPKVLSAILNFVKSFRHHQK
                     WEIGIVVGAGNIWRGKNAELIGFDPAAADYMGMTATFLNSLALKDYFTQNDLPAVVLN
                     AFAVEGLAEKTSPELAKKHLAKGEVVIFGGGTGKPFYTTDTGAALRAIDMNADLIIFV
                     KDGVDAVYTKNPHQYKDAQKITQVTYQELIDLKVEAMDLSALEVLNKTNITSFVIGMD
                     EVLNFEPLEEHMEIGTIITKR"
     gene            16179..16724
                     /gene="frr"
                     /locus_tag="PLU08_02050"
     CDS             16179..16724
                     /gene="frr"
                     /locus_tag="PLU08_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOQ70523.1"
                     /translation="MDEYALLAEERMEKSILNLQSEFSTLRTGRASAALLDRIECDYY
                     GEKMPINQISSISVPEPRQLLIKPYDKGDMRSILAAINASTLGINPINDGDTIRLILP
                     PLTEERRRDLVKQAKKFTEEAKIAIRNIRRDANTSIKNDKDLSEDMSKRAEEAVQKVT
                     DEYVKKADDLLKEKEEEILTV"
     gene            17087..17806
                     /gene="uppS"
                     /locus_tag="PLU08_02055"
     CDS             17087..17806
                     /gene="uppS"
                     /locus_tag="PLU08_02055"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOQ70524.1"
                     /translation="MSKTKITNYENYGIKHIAFILDGNGRWAKARKRPRTYGHEKGFS
                     SLFDVGIAVSKRNIPFMSVYAFSTENWSRPKKEVDFLFRTLEKRLHSVLKKVQKHNIK
                     IVTSGDLKRLPESTQQAINYAKEESKNNTGTTLNICINYGGKDEIVRAIKRLAADESV
                     NIQALTPDTFENYLDTSGMPPVDLLVRTSGEMRLSNYMLWQLAYAEFIFTPTYWPDYD
                     EECLVRDLEEYAKRTRRFGGL"
     gene            17809..18861
                     /locus_tag="PLU08_02060"
     CDS             17809..18861
                     /locus_tag="PLU08_02060"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOQ70525.1"
                     /translation="METNEIKPKTKRTSTIKISLNEISEATKKSMKTRTITSIILAVI
                     AVPALLLGGWYFLALVFFVSIIAVLEIMRGTKISKRYWCVYILAIVSTVALIFWIFIK
                     NNIMANIKAGYSWWDISKWVVENGFESIQVSSFLLATILIGLFILIVFDPHLGFDIAA
                     YLFLLVLFAGFGFQAFLFLRLYPQYIADRPDITFNTNIITSAFLIIYVILGTIMNDIG
                     AYFIGVLFGKNKMVPRISPNKTWEGFAGGVFFSIVVSFLFALIVSWIGYPVLPFLSHK
                     EWYYLLLISILMPLAANIGDLFFSATKRHLAIKDYGSILVGHGGILDRVDSLLMVSIV
                     TAMLIILINNGWSILV"
     gene            18874..20130
                     /locus_tag="PLU08_02065"
     CDS             18874..20130
                     /locus_tag="PLU08_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOQ70526.1"
                     /translation="MEFLSTILNIILFLVMLGVLITIHELGHFIAAKIFRVYVFEFAI
                     GFGPKIFRKKKGETYYSLRALPLGGYVAMLGEEDAVPDEFVNEEIPKERSLIGINRGK
                     RALIMSAGIIMNLVLGYAIFLFANGVLPQTGLSIRLEVKEDSPVYAMGIRSDDRIDFK
                     ETKIDGYTIQYFGEGKLDAAALEPEYYVLFQPKSLDDLTFGGESLVLLKIGATDLASE
                     NSFYTDLKDTDVLTFTATFLRGETYETAERIEKVLTLSTEPKNPDKLTDGYKFVSAGF
                     NLTKFKYRNNFSETFTAANKDFIRSVTAVARGLKSLFSDGLKNVSGPVGIFNISSQTL
                     KNHGLAPYLFLWGLISINLALFNLLPFPPLDGWHLLVVTVEAITRQEISPKFKQIASM
                     IGAILLIGLTLVILAKDIFGFVGVIL"
     gene            20131..24555
                     /locus_tag="PLU08_02070"
     CDS             20131..24555
                     /locus_tag="PLU08_02070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF001688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HOQ70527.1"
                     /translation="MGNVSARFFASINYENDKKIGLTFLEWKKVDEVWHYTLLKEKPW
                     NFEDYLDFSDALMSIPYAYKVSYAYTKLPRIEDALALFKPWHLHTYLSESNYVIMVVG
                     NDTLRFTFTDEVSALDAKAVLYEFSLLLKELSYNFALETVVISSAQEEDEEDEFSLGS
                     ERPFIEDDDDDEEYFDEEDDTGVADCDPLDPNENCERSLEYHDGLEDKLKENYEKMVK
                     ERQKRRHAKGAFNLTEIKDLEHKEVDEGVDFDGEIYSYDRRPTKHGVTYTLGIGKDAH
                     GISVRFYINKRGAKQDEVLKALKTGRFVRVQGYIAIDNFRGQTKIVRGRDIDEIAAPP
                     LPTDDYEGRKRIELHLHTKMSALDAVSTIDEYCELAKNMGHEAIALTDHGVVQAFPEA
                     QKAGKKYDIKIIYGSELYVIDETLKVVNNPAPIILNNARYVIFDLETTGLSARYNEII
                     EFGAVRVEHGLVTARLDILINPGIVVPEHITRITKITNAMLRGKPKLDDVLDEILEFL
                     GDDILVSHNAEFDLGFLNANLRKRGRNPLNNPTIDTLALSRYLFPESRGHRLGVLARN
                     LEVFYDEDGAHRADYDAEVLNSIWQAMLTKLTEDDQYLTHAALAELKLTQAALQYLWP
                     AHATVYVKNKDGLRDLYKLVSVSHTEFSTDIPKTPRSMLLSHRDNLLIGSGCFNGEVF
                     ETAARFDEDRLLKVMEFYDFIEIQPYSNYSYLINMGELDSEEHLKKVIGDIKSAAQKL
                     GKPLVATGDVHYCLPSQKVFRDVYIVAKGLKNVNHPLNPYDRDKRPHFENPDQHYRST
                     TEMFEEMRKFGVFTEEELADMIINNPHRINDLVENITPFTKELYKPSIENVDELLENL
                     VFETAYEIYGNPLPTLISDRIHRELTGIINNGYAVIYYLAHLIVKKANDEGFIVGSRG
                     SVGSSVVAYFAKVSEVNPLPPHYFCPHCKFSNFRNEQRVLSGYDLPLAKCPECGHDLV
                     GEGQNIPFETFLGFEADKIPDIDLNFPEDFQKEAHNFTKELVGENNAFRAGTIQTVAE
                     RTAFGHVLNYYEKQGIYRDELNNENVAYLAYHIAGVKRTTGQHPGGIVVIPSEYEVYD
                     FTPVQYPAGNTESDWLTTHFDYDSIHDNLLKLDLLGHKDPVTLKMLCDLTGIKFEDVP
                     TNDRATISLFSSHEALNLKSNPLGEVTGALGLPEFGTNFVRQLLTEAKPKTFNDLVIV
                     SGLSHGTGVWRFNADELIRSEQATLGDVIGCRDDIMTFLIRQNLHEKDAFNIMESVRR
                     GRGVEEGLETKMREAGVPHYYIESCKKIEYLFPRAHAAAYVIMAVKVAWFKLNYPLEY
                     YATFFTIRGDNFDLKTMISSEEVILKELQKFEEQRKTSELNPRDSNIVENLQLTIEML
                     NRGFKISNIDLYKSEATRFKVDHENNQIIPPFIVIRALGEGTAESVVEARKNGEFISI
                     EDLVERTRLNTSNIENLKELGALEGLPESNQISLFDFM"
     gene            complement(24557..25465)
                     /locus_tag="PLU08_02075"
     CDS             complement(24557..25465)
                     /locus_tag="PLU08_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70528.1"
                     /translation="MNKESGYRKTIISEYLNNLLHALNAYEGYSDGFRKLNGDFINYL
                     QARWVDLNIEVGDAIRVKFQRDRDYLSHPFLGIVIRLLEGARIHVPQREETLALLKKH
                     TNYPLHNFITFAFNFNRNDKDAPFEILSDDFIKIIFYLTNKKGYPHLENFDSFLEYKL
                     RISGVSIINLVYARRSALVEKNIDKVTEHLSLAAIQSGQDHRKFLGQKYYELATLFKK
                     HNDFAGKLLQKAVDFKNYEALLPLVKYYEEASDDKDDYTAIAFYYLVIAHHLEIPGAG
                     EKLKTYYEKGGVRPPCNKMLSKFFED"
     gene            25579..25655
                     /locus_tag="PLU08_02080"
     tRNA            25579..25655
                     /locus_tag="PLU08_02080"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25613..25615,aa:Pro,seq:cgg)
     gene            25784..25870
                     /locus_tag="PLU08_02085"
     tRNA            25784..25870
                     /locus_tag="PLU08_02085"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25818..25820,aa:Leu,seq:caa)
     gene            26066..26455
                     /locus_tag="PLU08_02090"
     CDS             26066..26455
                     /locus_tag="PLU08_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70529.1"
                     /translation="MNKTNLKNKISKRIIISEIIYVVVTAIISLLMFLPIYKDRATLP
                     GYDEEGNQIVVNLFYEKTPYQRLKAINIEWLLYLGLSLFLICIVILIISYTTNHKLDK
                     YKKVIFIISFGLILVLLLIAAIQITMY"
     gene            26751..28037
                     /locus_tag="PLU08_02095"
     CDS             26751..28037
                     /locus_tag="PLU08_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOQ70530.1"
                     /translation="MNLKPEKYKKRIIDQQIEKYLNIFGAVCVEGPKWCGKTWSSLTH
                     SKSVIYIGDPEGNFQNRTLAEIDPKGVLNGDSPRLIDEWQEVPYLWDAVRAEVDFRGE
                     KGQFILTGSSTPKRKGVLHSGIGRIHTLRMKTMSLYESLDSSGEVSLLSLFDNEIKTN
                     LTKQFSLKELIELVVRGGWPMSLEQDKDFYGEIPKSYVNTLLNTDIHEIDNISRNKDK
                     FEALLKSLARNESTLAGNRVLINDINKYEKESIDDKTLVEYLNVLERLFIVENQNAFD
                     PNYRSSKRVARTPKRHFIDPSLAVAALGMSKEMLLNDLEYFGFLFEALVIRDLKIYAE
                     SIGGKVYHYKHHDTNHELDAVVELNDGRWIAIEVKLGASQIDDAAENLLKINNYFVNN
                     ENTSKPSALVVICGLSKAYYKRKDGVIVVPITALKP"
     gene            28053..28271
                     /locus_tag="PLU08_02100"
     CDS             28053..28271
                     /locus_tag="PLU08_02100"
                     /inference="COORDINATES: protein motif:HMM:NF040441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PF20097 family protein"
                     /protein_id="HOQ70531.1"
                     /translation="MKINNCPYCNKDLKTGKLVYRYAYRAPKIIFNDGSEKKIDKLKL
                     FKDAVINKVFYCESCNIMLADLDSDSQK"
BASE COUNT         9515 a         4651 c         5535 g         9013 t
ORIGIN      
        1 gtaatcgttc aatcatacaa ccccctagat tattatcatt cagacagagg actttcgatg
       61 taagaatagc ggcgtgcaaa gctataattt ttgttgctat tttcttgcta atttagtatg
      121 caatttatat agattatgaa agtttatgtt tataattttt caagttcatc taaaagcata
      181 gtgtctttgt caggaatcaa ataatctgct tcgtttgcat taattatttt agaactaata
      241 agaacatcaa catataaatc taaaaaccgg tcatatggtg tatcaaatct attattgaga
      301 ttatcttgaa tgtctttatc taattccatt tttttgaaaa gaagtgctaa aaaaggatag
      361 ggattacgaa tatcgcattt accaaaatca taaataaaca accacgtagc aaaattaaat
      421 gctgtttttc tatcatgcac ctcatgtttt aaatacttca ttcactccgc tactaaggtt
      481 tcccagccaa atgtagaaac tagttggtaa acagtatttt taattttttg tatatttggt
      541 atttcttcac catcaataca attttgtgaa aataattcta tgtttagaat attttttact
      601 atgctcttat catttaactt gatttattaa tagttaaatt tccttaatcc tatttctcat
      661 caaatttatt ataggcattt attccgtgtt tttcagaagt attattgcct attttgtaaa
      721 tttataaatt atcttttttt ctaagttttg ttttttgtga agcgtgaccc tttttcaata
      781 gcgtctttct cccattcacc aataacgtcg tcatcaattt ctttaagcag ttttttaatt
      841 tcgttatctt ttgtaatctt tttaaaaaga tcaggccgac gttcgcgagt aatacgtaaa
      901 ctctctttta gccgccactt acgaatcgct gcgtgattac cagaatacaa aatagtagga
      961 acagtatatc ctctaaagtc atatggctcc gtatattgcg gatattcaag taaatggtaa
     1021 tcaaagcttt caatatctaa actttcggtg ctaattactt catcaagtaa acgaatagca
     1081 gcgtcactaa tgaccatcgc gccaagttca ccgccagtta agatgtagtc gccgattgat
     1141 atttcttcat cgataaagtt attcactctt tcgtcaattc cttcataatg gccacagata
     1201 aagattaaat gttctttttg acttagcgct gttgctttca tattagtaag gacattgccg
     1261 cgcggtgaaa gtaacagctt atgaccagag tcaacacttt caagtgcttt aacaattggt
     1321 tcagcttgaa gaatcatccc tgctccacca ccgacaggtg gagtatcaac tctattccat
     1381 ttgttatcag cgaagtcacg gatattaaca atatcaaaag ttacatggcc ttgaagtctc
     1441 gcttttttaa taattgatgt ttcaaggaaa ttggtaaaca tttcgggaaa taatgttaat
     1501 attgtaatct tcataacata ccctcaattg gtcgaaatac tattgttttg ctcgctaaat
     1561 cagtggaaac gatgaattct tcgatatatg gaattagaat attactaccg tcttcccttt
     1621 ttactcttaa agtgtggtaa gcagcgtatt caagtatttc aataacttca cccacaagtg
     1681 tttcatcttc taaaacgact ttcatcccta aaaggtcatc aaagaaaaag taaccttctt
     1741 caagggtgat gtctttctta gcgatgaaaa gaaatccttt tagataattc tcggcaatat
     1801 tgatgttatc aatttcggca aaggtaacta ttaatagtcc tttttgttca cgaacacttt
     1861 gaatggttaa tgtttcagtt ttagaagtag tattattttt tactaatact ttattaccaa
     1921 cttcaaaacg ttcctccgca aaagaagtag tcggtttaat tttaagtgca ccctttaagg
     1981 catgcgtttc aacaatataa cctattttta aataatcttt gttcataatt ttattatatc
     2041 aatttaaaaa aactctcgag tgagagttat ttattttcgt ctagtgcttc aaaacgtaag
     2101 aagacatgtt cttcgtcaag tttaccggcg acagacacta cttcacgtaa tgcattagcg
     2161 acaacaccgc gacgtccaat taagacggca atatcatcac tttcggcaaa gatgttaata
     2221 ataatacgat ggccttcgtt agtttgacga ataagtaaag cttgttcgtt tttcactaat
     2281 ggggtaataa tcgtgcgcac taatgcttcg tagtcacgca taattattta cctgactttt
     2341 ttgattttgc gaattcttcg ttaatgcctt gggcgcggaa taaagcttta acggttgcac
     2401 ttggttgtgc cccatttcct aaccatttaa gcgctaattc gcgatcgata acggcttctt
     2461 taagatcttt ctttggatca tagtgtccaa tttgttcgat aatacggcca tcacgggcat
     2521 agcgactatc agcggcaaca atccgataat gtggatcatg agtgcggcca acacgggtta
     2581 atctaatttt aactgccata taagtaatcc tcctttttat accgtgataa gtttagcgtg
     2641 actcgtttac ttagtcaagt aattttactt aactttagtt aaattagtat gtatatcttt
     2701 tatttttctt aaaattgact aaaataggta tattaaaaac aaggagcaat ttatgaaaaa
     2761 tctactaaat aagctgttca agtttgaaga aagaaaagca aacttgacaa gtgaaatttt
     2821 aggaggatta gtcacctttt tagcgatgat atatattctc ccaacaaacg ctggtatttt
     2881 aagtgatatg ggcatggaca aaagcggagt cttcgttgct actgccatcg ttagtgctat
     2941 tgttagttta gtgatgggac tttacggtaa tttcccgatc gtcctaagtg caggcatggg
     3001 ggttaacgct tacctagctt acgtagttta tggagcgcta ggttcatggc aagcagcctt
     3061 gcttgtgatg ttttttgccg gtattatttt tgcggtgatt agtctttcac cattacggaa
     3121 gatgatgatt gatgcgattc ctaatgacat caaatatatt atcagcgcag ggctaggtgc
     3181 cttcattgct tttgtcggct ttaaaagcag tggttttatt gttgatagtc ctgcgacttt
     3241 agtgtcactt gggaacttac gtgatccaat ggtcttactt tctttattcg gtgttttact
     3301 tgttgttatc tttatgtttt ccagcaataa gcggttaaat caactcgcaa taccaattgc
     3361 aatgatcatt gttgttgttg tttctgtcgc ggtgtggaat atctttgcat taccagaagc
     3421 aactgacgct gggcgtccaa ttgctttcca ttttactaat attaaagata tgtttagtaa
     3481 tccaacttca tggggtggac aaggttttga aaaagtcgct tttaagattt tcgataaaga
     3541 aacatggggc actgctttaa gcaatccttc aacatacgcc tttatcttct cactagtact
     3601 cgttaatctt tttgatacga cagcgacaat tttagctgtg ggacgtggcg ctggtattat
     3661 cgacgctgat gggaagatgg ttggtggtca taaagtagtg gccgctgatt cttttggtgc
     3721 cgttatttgt gcaccgcttg gtacaagtac agtaacttca ttttccgaaa gtgggattgc
     3781 tgttgaaatg ggtgctaaga ctggtttagc ggcggttgta gcgggattta tgttcttact
     3841 tagtgctttt atttacccaa tcttttatat ttttaccgaa ccagcggtta caagtatggc
     3901 acttatcagt gttggtggct taatctttgt tggtaactta aaagatgtta actttgatga
     3961 tcgcattatt ggttttacag ctttctttac aattatgctt attttactta cttactcgat
     4021 tagtgatggc ttaggtttag gacttataat gtatgtcatt atgatgtttg ttagtaagcg
     4081 tggtaaagaa gttaaaatac cgatgtatgt tgtagcggga gtatttgtta tctactttat
     4141 ccttaaagct ttagtttaaa cataagttta ataaacgcta atagcccact ttacgaaaga
     4201 gtgggctttt ttaaaatttt agtaattact ctttacataa gagtagtctt ttggtatatt
     4261 aatggagcgc aaagtcgctg tggtccgctg ccaaaaggta agaacacgaa aataatattg
     4321 caggaaggag cgagttattc atgaataaaa atttagttaa tgaaattaac gcttctcaac
     4381 ttcgtaccga tcttcccgaa ttcagtagtg gtgatactgt caaggtcgct gttaaaattc
     4441 gtgaaggtga caaagaacgg attcaatttt ttgaaggcgt ggtcattgct atgcaaggcg
     4501 caggcgtcaa ccacacattc actgtccgca agatgtcaag tggtattggt gttgagcgtg
     4561 ttttcttcac tcattcacca gtcattgaca gtgtggaagt tctccgtcgc gggaaagttc
     4621 gtcggaacaa aatttattac attcgtaaat tatctggtcg taaagcccga attaaagaga
     4681 agaaataagt taaaatcatt tatgagagaa agccagaaaa ctggcttttt ttcttataaa
     4741 acgagtattt atttgtgata ataagtagga aaatgtacat caaatgtaca tttcgttata
     4801 taattactat atggtcatag aagaagtact tattaatgaa gaaataagtg aagcagagat
     4861 aaatcggaaa aataaaccca gactacgtaa agcgcttagt gtgacgtgga ctattttact
     4921 cgtgaccgct attgttgttt cgctttttgt gacttatgtt gatttaactt acaccccagt
     4981 ttatgttgat ggaccaagta tgcaaccaac actcaatgac tttcatcacc gctattatga
     5041 atttggctta atggacgaaa gaccaaaagc ccgcaacaat attaagcgtg gtgatatcgt
     5101 tgtatttcgg cgtaacggta ttaacattat taagcgtgtg attgctttgc cactagaaac
     5161 tattcaaatt attgataacc cgacagggga cgaagttttt attacactgc ctgaagcaac
     5221 cgagaaattt aaactcgatg agccctactt atcaacaagt aatcgttata caaccgcaac
     5281 atatttaaac gatagttctc caaaatatga aaacggactt aaagaaccgt taactttaga
     5341 agagaaaact tattacttaa tgggtgataa ccgtgcaaat tccagtgatt cacgggcaat
     5401 tggtcctgtt cattttgaca atatcagcgg caaattagtc gtaattcaag gttatacgga
     5461 aaatgttgaa gttagtgaat caggtaaagt taaatttatt ggtcagcatt attatgtgct
     5521 gtggaaatgg cggtactact aatgagtaaa caaattggtt ggttcccagg tcacatgcaa
     5581 aaagcgacac gcaagctcat tgaaacgcta aagatggtcg atattatcgt tgaattaggc
     5641 gatgcacgga tcccccatag tagtcgcgct acttacttaa cggcgattat tgaacgaaag
     5701 ccaaatcttt atgttttaac taaggccgac ttagctgatc ctaaaagagt aagtaaacat
     5761 acagaaaaat taaatgtttt agcgggtaat ttaaatgaac ggaaatttgt gcgacaaata
     5821 ttagcaaaga ttaaagaaat cgggcaacca ataattgaac gtgatgtgcg tcgcggatta
     5881 aaaccacgac ctttagcggt aatgattgtc gggattccta atgttggtaa atcaacactt
     5941 attaataaac tagcgggacg caaagccgca agtgtgcaaa ataaaccagg gcatactcgc
     6001 gctaatcagt atattagagt gagtgaagaa attgccttaa ttgatacacc aggaatttta
     6061 tcacctaatt atgatgaaga agatacaagc atgaaattag cgcttatagg tacaattaga
     6121 caagagattt taccaacgca tacaatggca gagtatttag taaattactt aaaaacatat
     6181 tactttgaag aaatggtagc tttttacgat attgatatta aagaagatag taactaccaa
     6241 accgtgatta gtgctattgc tacgcgtcgc gggattattg gtgaagattt tgatgttagt
     6301 gatcgcaccg aaatcttaat cttaaatgaa tttaaaaatg ggctaattgg ccgaattagt
     6361 ctagagtagt aaattagtga aatataaaaa acgaccgcta gtggataacg gtcgcttttt
     6421 aataggcgag accttagttt tagaccacag gttttatgac aatttttgtc gcatcaaaga
     6481 agaagaacgg ataatcgtta gcggtcacat taatccgcac tttgcctgaa gtacttacag
     6541 cgtttgaccc atttactgta taaccttcat tttcaagact gccgaaatag ctatcaacaa
     6601 aattaacacc aggcgcacaa acgataaatt cattacgctc tgttaattca gcgaaatagt
     6661 taccaccagt aagactagga actctttcgg taataccgta tttacttaag aaagtttgaa
     6721 gttgattggc cggccatccg atatcacttt caagcttatc aaagcgaaat gaatcagcct
     6781 taagcgcact aataacaata tacatagcgt aatcaagaac tggatgatct tgcatgtatt
     6841 ggtaggcaat attaattgtg tcttcggtgt taacagcaat actagtccaa ctaagtttac
     6901 tgtatgtgta gccagctcct tcaagtatgg ctttataatc tgcttcgcga ttagcaccag
     6961 gtactacaat cgcaaagtac ttattattag cgtcattatt ttcatgataa taagtagtgg
     7021 cgatatttgt taatgctgga acggtttcat taactctttc gcttgataag aaagtagcaa
     7081 gcttttgcgt tggccagcct tctctttttt cgtaatccat attttcaaga atatcttctt
     7141 ccgctacatc gttagtagtt ttttgactgc ttttatcgtc gccatcaata atatcttcag
     7201 gaccgtctcc accaaagcat ccagttaaga gcaatcctaa aagtagtggg attagtaaaa
     7261 attttcgttt tttcataatc ttctcctttc actaatctat attttcattt taatttttaa
     7321 aaatgaaatc tcaatactag cttaggcatt ttaggaacaa aatcacaaat atttgaaata
     7381 ttaaaaatag aaatagttag tttctataat ataataatgt tagtaattga attactagct
     7441 tgtaagtttg gagtgataat atgacatatt ttgaatttga taatgatgta agaaaacacg
     7501 gacaccttta tttagtggga gttgatgaag cgggacgcgg ccctttagca ggacctgtgt
     7561 ttgctgccgc ggttattttg ccgccctttt ttaacgatga gcgaattaaa gactcgaaac
     7621 aattaacagc aacggagtta cttgaattaa gtgacttaat taaagagaag gctcttgatt
     7681 accaaattgt gcatgttgac gctcccgtta ttgatcaagt taatatttat caagcaacaa
     7741 ttttagcaat gcaaaaagca gttactggct taagtcatga atttcatttt gtgttaactg
     7801 atgcgatgcc gctatctaat ttagaagtgg ggtacgaagc aattattcaa ggagatgcga
     7861 cttcgcttag tgtggccgcc gcaagtattc ttgctaaagt agcgcgtgat gcttatatga
     7921 ataaactcgc gaaactctat ccgcaatatg attttgaaaa taataaaggt tatccaacac
     7981 ggaaacattt acaagcactt gatacttatg gcccaattaa agatgtgcat cgctactctt
     8041 ataaaccagt gcaagatgcg ctcaataaac aaattaaatt attttaatgt gataaaaaat
     8101 taagttcttc cttatttata taatgaggaa ctacaaatga ataaaaaagc acgcgatgtt
     8161 ttactttact ttgccagtaa atatgaaggc aattaccacg caattattaa tgcactccgc
     8221 tccaaagaac cagttgatga cgctttattt aataaatata aagaagactg ggaaaaagtt
     8281 gataccataa cgattattga tgaagactac ccagcgcttt ttaaaacagt taataatccg
     8341 ccaattgttt tatttttaaa aggtaacaaa gaattacttt ttaatttgga aaaatctatc
     8401 gctgtgattg gtgctcgaga gtatagtgaa tacggtaaaa acatgactta tgaaattgtc
     8461 gaaggactcg ttaaagaaaa atttacgatt gttagtggct tagcgcgcgg tatagacggg
     8521 tttagtcatg atgcgtgttt acgggcaggt ggcaacacaa tagcggtcct aggcagcgga
     8581 attaattatc cttatcctaa atcaaataaa aaactatatg acgaagttag taagcacggt
     8641 cttttaataa ctgagtatcc taatgataca aagccagaac cagattactt taaatttcgc
     8701 aatagacttg tagcggcgct cacaaaagcc gttttaatca tcgaagctaa atatcgctca
     8761 ggcacaataa taacggttgg ttacgcctta gaaaaaggga gtgatgtttt ctgtgttcct
     8821 gagcgcgctg gccgtaatag tggctgtaat agactgataa aagatggtgc ttatcttgtc
     8881 gagagcgcaa gtgacattat aaaactctgg ccaaactaag aaaataaaaa atgttttatt
     8941 tattataaat aaagagaaaa ttcacaaagt tttacatttg acaagcgcaa aaaaaccctc
     9001 tatattttat aaccgatagg aggctttgtg aaactagtaa ttgttgaatc cccaagcaaa
     9061 attaaaacga tcgaacgcta cttaagtgac gagtacaaag tactcgctag ctatggtcat
     9121 atcgtcgacc tttcaacacg tggtcgcaag aatttaggta ttaatcttga aacttttgaa
     9181 ggtgactatg tattagataa gagtcggcaa aaagttgcag atacgctaaa aaaagctaaa
     9241 aatgaaagta gtgaagtcta ccttgccact gaccctgatc gtgaaggtga agcaattgcg
     9301 gtgcacatcg ctaaactttt aaaccttgat ttaaatacga ctaagcgtct tgagtttcat
     9361 gagattacgc gggacgggat tactaatgcc ctcgctgcgc cacgcttgct taatgaagac
     9421 ctttttaata gccaagaagc tagaagaatg cttgaccgta ttgttggctt tgaactgtct
     9481 agtctcttaa atcaaaaaat aaatgttaaa agcgctgggc gagttcaaag tgcggtttta
     9541 aaactaatcg ttgataagga aaacgaaatc actgcttttg tccctgaaga atattggaca
     9601 ctaaaaattg acactaaata tgacgatgct ctttttagtg tgaattttaa taagagttac
     9661 gctggtgata ctaagattgt taacgaagcg atgaaaaatg atatcgttag taaactagcg
     9721 cctaaaattg ttgtgactga tgttaaaagt gaaattaaac acactaatcc taagccgcct
     9781 tttaccacat caaccttgca acaagaagca agtagtaaat ttaaatatag tgcttcccgc
     9841 acttcacgga ttatccaaaa actctatgaa ggtatacaaa ttaacgggat tgaaacgggg
     9901 ttagtaactt atattcgtac tgactcgaca agactcgcgc caagctttgt cgctaatgca
     9961 tcacaacgca tcgttaaaga atatggggcc gaatatcttg gtcgtactca agcgcgtggt
    10021 aaaacactac aaaatgtcca agatgctcac gaagcgattc gtcccacgaa cttaaacttt
    10081 actcctgatt caattaaaga ttcattaacg agagaagaat ataatttgta ttcacttatt
    10141 tacacacgag cacttgcaag tttaatggtc ggtagtaaag aacaagttac gcgttataca
    10201 tttaattcaa atggtactat tttcaatgcg acatcaacaa tcttaatatt cccgggcttt
    10261 agaaaaattt atgggaaaga tgaagaaaca gtagatgact cagcgcttcc gcaaatgcat
    10321 gttggtagtg aattaccaat tattgatgtt ataactgaac aaaaatttac tgaaccacct
    10381 gcgcgttaca gtgaaggccg cttaattaaa acgatggaag aactcggtat tggtcgacca
    10441 tcaacttatg caccaacgat tcaaatatta caaaaacgta aatatgtatc tagccttaaa
    10501 ggtacgctta agccaacgga tattggcttt agacaaattg atgctcttag cgaatttttc
    10561 ccaagtatta ttagtagtga ttacactgct tcaatggaaa atgaattaga taaaatagca
    10621 aatggcgaag gcgataaagt tgaatttctt agtgatttct acagtgactt tagtcaaaaa
    10681 attgatgatg cgcgcaaagg aatgaagaaa ctaccgccag aaaaggtcgg tcgcgattgt
    10741 ccaaggtgtg gctcaaactt agtgtaccgc gaaggaccat atggtcgctt tattggctgc
    10801 gaaactttcc cgaaatgtaa atacaccgaa ttcattgttg aatacactgg tgaaaattgt
    10861 ccaaaatgta atgagccttt aacttatcgt tatgattcac gtaatcgtaa atatattcgt
    10921 tgctcaaatt ataaaagttg tgattattcc gcaactatta aaagtactag acgtaaaaaa
    10981 taacttattt ttgtagggaa attgccgttt tcttgctata atctcattgt ggaacaagta
    11041 gtaattattg gtggtggatt agcaggagta gaagcggctt tttttcttgc ggaaaaaggg
    11101 attaaagtta agttatatga aatgcgtcct ggtgtcatga cgcctgctca tcatagcgcg
    11161 aagcttggtg aacttgtgtg ttctaattca ttaaagagta aagaacttac aaacgcatgc
    11221 ggtttactta agaaagaaat ggaaatatat aattcactaa tgacaagtgt tgctgcgcgt
    11281 gttagtgtcc ctggcggtaa tgctctttgc gttgatcgtg aactttatag cgaagaagta
    11341 acaaggatta ttactactca tccgcttatt aaagtagtga acgaagaagt agtcgaactt
    11401 ccttctcatg ttcctgttat tattgccagc ggacctttaa ctagtaaagg cttagagaac
    11461 acgattagta aacttatcga tacagcgttt ctacattttt ttgacgctag tgcaccaatc
    11521 attgaaaaag attcaattaa tatggatatt gcttattata aatcgcgcta tgatcaaggt
    11581 gacgatgctt atattaattg tccatttaca aaagaagaat attatcgatt ttatgatgaa
    11641 ttaattaacg ctaaactagc gccaattcat gaaatggata cgaaatactt tgatgcctgt
    11701 atgccgttag aagtaatagc gaagagagga cctaaaacct tacgctttgg accacttaaa
    11761 ccacgcggac ttggtaaaga tgagcatcat aaaccttatg ctgtccttca acttcggcaa
    11821 gataatgtaa ttggctcgct ttataactta gtcggctttc aaacaaactt aacttatgct
    11881 gaacagcgcc gtgtatttag acttataccc ggtttagaaa acgctgagtt tgttcgttac
    11941 ggattaatgc accgcaatac atttgttaat gcaccgcgtg tcctaaattt agataagtca
    12001 cttaaagtta acaatgatat ttatattgct ggtcaactta gtggcgttga aggttatgtt
    12061 gaaagtgccg ctagcgggct ttatgttgcc cttaatgttt acgctaaaat aaaaggtgta
    12121 aaattagagt atccgtgtga gacaatgctt ggtagtttaa tgcattactt aaataacgcc
    12181 aatccagaaa actttgctcc aatgaacgct aattttggaa tagttcgagg tgctactaaa
    12241 caaaaccgca tggaaattgc cgagaaatca ttaaaaataa cgcaaaaata taaggagtac
    12301 attgagtcat taaatgaata gacctttaca agagtttcaa gattacttac gttacactag
    12361 acgcttttca gaaaacacag tcattgctta tataagtgac attgaaactt tttatttatt
    12421 tctcgctaat cagaatatgc gtgacgacga ggttgataaa ccattaatcc ggttatattt
    12481 aaaagatcaa attgataaat atgtatcgcg gcgcagcatc cagcgccgac tagcaagctt
    12541 acgccattac tattcatttt tacaagataa gcgctatgtg aaagataatc cctttttaac
    12601 agtacaagca ccaaaagcaa caaaagcatt gcctcgcgtg ctttatgaag aagagattcg
    12661 cttaatctta ctcgctaatg aaaaacgcac tgattattta agggatcgtg atcaattaat
    12721 tattgaatta atgtttgcaa cagggttgcg tgctagtgaa gttattaatt taacacttca
    12781 atcttttaac ttaaatgaac gctttttaat ggtacttggc aaaggtaaga aagaacgaat
    12841 ggttcctttt acagaccgag tacaagtctt attacgcaat tatttaaatg gtttacgcaa
    12901 agaactttta gcgaaaagaa aaagtagtca accgacaaac attttatttc ttaatgccca
    12961 aggtaataaa ttaacaatcc aagggttacg ctatatattg ctttcgattg aaaagaagac
    13021 gggggagtat gttaacttac atccgcactt actccgccac tcttttgcga cttatttatt
    13081 agaaaaaggc gctaatttac gggtcatcca agaattatta gggcatgagt caataaacac
    13141 aacacaaatt tatacgcatg ttagcgaaga aaaaattaca aaagagtact atactgccca
    13201 tccgcgcgct aaaaagaaaa tagactaatc acttcattta actttataag ttaactagtt
    13261 gcctaaacct tttacctatg gtaatatact aatggcccaa aaaagggcta atacgcacac
    13321 attgaattcc tcgtccggtt ctacaggtgt agggacaagg ttcgaaaatg tggaggaaat
    13381 aacaaaagga ggctcaaaca tgagtgaaga agaaaaagtc gtattaactg aggaggaagt
    13441 aactcccgca atggaaaatg tcttaccaga tgaaaatcta ccagtcatta ccatgaaaaa
    13501 attacttgaa gctggggcac attttggtca ccaaaccaaa ttctggaacc caaaaatgaa
    13561 accatatatt tatggttcac gcaacaatgt gcatatcatt gacttagaaa aaacaattgt
    13621 caaaatcgaa gaagcttacg ctgcattacg cgaaattgtt actcgcggtg gtaaagtctt
    13681 attcgtcggc acccgtaaac aacatgcaga acacgttaaa agtgaagcgg aacgaagtgg
    13741 ttcattctat atgaataatc gttggttagg tggtacatta acaaacttta gaacaatttc
    13801 aactcgcgta aaatatttac gtgatttaga aatgcaagaa gctgaaggtg cttttgatcg
    13861 tctaccgaag aaagaagcaa tcttaattcg taaagaaatg aaacgcttaa atgataactt
    13921 tgaaggcatt aaagaaatgc gcaaagttcc agaagcagtg tttgtcgtta gcacgattga
    13981 cgaagcaaac gcaattagag aagcacgtaa cttaggtatt ccagtgttcg cacttgtcga
    14041 tacaaactgt aatccagatg tcgttgatta cattattcca agtaatgatg acgctactaa
    14101 aaccgtgcaa ttaatgctta ctttatttgc tgacgcgatt gttgaagcaa aaggtggtat
    14161 ccctgtttta gcatatacac gcgatgctga acctgaaact gaaacattag aagaagcgtt
    14221 aaaacataac gaaccacgtg cccgtcgcac tgactccgaa gaacggcgtc aagttcgcaa
    14281 agcgcagacc caacacagcg ataaaccaac acgtgttcgt aaaactgaag ctaaagaaga
    14341 aggtgaagct gctccagaag tagcggaaaa acctgctgct gcagctaaac caaaaaaaga
    14401 aaaagaagtt aaagaaccga aagctgaaaa agctgaagag ccaaaagtga aagctgaacc
    14461 aaaagctaaa gctccaaaag ctccaaaagt tgaagaagtg aaaactgaag aagtaaaaac
    14521 tgagaaagaa ggagaataat taatggcaag tttaatcgat aaaattaaag aattgcgtga
    14581 acttaccggc gcaggaatga tggactgtaa aaaagccctc gttgaaaacg gtgaagatgt
    14641 tgataaagct aaagattggc ttcgtgagca aggtatcacg accgctgcga agaaaagcgg
    14701 ccggattgct gctgaaggct tagcgcttgc aaaaatagaa gatggctacg ctgctattgt
    14761 tgaagttaac tgtgaaactg actttgttag tcgcggtgat gactttaaag cgttagtgaa
    14821 cactgttctt gatttattgc tcaaacataa acctgctaat aaagaagcag cggatgcaat
    14881 tattaatcca cttttagttg aagcaacagt aaagattggt gaaaaattat cattccgtcg
    14941 ttttgaactc gttaaggttg aagaccgtgg tgcaacttac attcatatgg gtggtaaaat
    15001 tgctactgtc gtactcttag ataagaaaaa tgacgaatta gcgcatggct taacaatgca
    15061 cattgctgcg aatagtccaa gctatattac cactgatgat attaccgaag aagattacaa
    15121 tcatgaacgc ggtattcaac ttgaaattac taaaaacgat ccacgtttag cagacaaacc
    15181 agaaaaaatg cttaaaaaca tcgttgaagg taaaattaga aaatacttta gtgatttagt
    15241 cttagccgaa caaacatatt taatggacga tacaaagaaa gttggtcaag ccctaaaaga
    15301 tcttgacaat aaagtcttag tgatgttccg ttaccaagtc ggtgaaggaa ttgaaaaacg
    15361 tcaagaagat ttcgccgctg aagtctttgg tcaaattaaa taataaacca caaaaagaaa
    15421 acaccttcgg gtgttttttt ggaggaaaaa agtatggtat ataaaagagt gctcttaaaa
    15481 ctaagtggag aagcccttgc cggtgataaa aaaagcggaa tttatgatcc aaaagtatta
    15541 tccgcgattt taaattttgt caaatcattc cgccatcatc aaaaatggga aattggtatc
    15601 gttgttggcg ctggtaatat ttggcggggg aaaaacgctg aacttattgg ttttgaccct
    15661 gctgctgctg attatatggg gatgactgcg acatttttga actcactagc gctgaaagat
    15721 tattttacgc aaaatgattt acctgctgtt gtcttaaacg cttttgcggt tgaaggactt
    15781 gcggaaaaaa ctagtccaga attagcaaaa aaacatttag cgaagggaga agttgtcatt
    15841 tttggtggtg gtacaggtaa accgttctac acgactgata caggtgcagc actccgcgct
    15901 attgatatga atgctgacct aattattttc gtaaaagacg gtgttgacgc tgtttacact
    15961 aaaaatccgc atcaatataa agatgcacaa aaaataaccc aagtcactta ccaagaacta
    16021 attgatctta aagttgaagc gatggattta agcgccctag aagtactaaa taaaacgaac
    16081 atcacaagtt ttgtcattgg tatggatgaa gttttaaact tcgaaccact tgaagaacat
    16141 atggaaattg gtacaattat aacgaagagg taaaaaatat ggatgaatat gcattactcg
    16201 ctgaagagcg aatggaaaaa tcaattttga atttacaaag tgaattttca acactccgca
    16261 ccggacgggc aagtgcggcg ttacttgacc ggattgaatg tgattattac ggtgaaaaaa
    16321 tgccaattaa tcaaattagt tcaattagtg tccctgaacc gcgtcaactt ttaattaagc
    16381 cttatgataa aggtgatatg cgttcaattt tagcggcaat taacgcctca acactgggga
    16441 ttaacccgat taatgatggc gatacaattc gcttaatctt acccccactt accgaagagc
    16501 ggcgccgtga tttagtgaaa caagctaaaa agtttacgga agaagcaaaa attgccattc
    16561 gtaatattcg gcgtgacgct aatacgagta ttaaaaatga taaagattta agtgaagaca
    16621 tgagcaaaag agcagaagaa gctgtgcaaa aagttactga cgaatatgtc aaaaaagctg
    16681 atgatttact taaagaaaaa gaagaagaaa tcttaaccgt ttaatgtaaa ttaacaacta
    16741 tgaattaagc cttttacaag gcttttttct tttgtaacac actagtgtgt tatcctaaaa
    16801 ttaatataag agccaaaagt aataagtaaa tcgcggaaaa cattattatt ctagcgaaga
    16861 agacctgcgg cttatgtggg ttaagaagcg aaaagtctat actgatacaa tcataaaatt
    16921 taatgaaaaa gtagcgatct ctttcccatc ttttgataaa gattatccgt tgtatcctta
    16981 ttaaagctat tagtgaaata aattattttt acaaacgctt ttctttttta acattttaaa
    17041 aatgttattt aatgaaaata gttattctta ttgtataatt taacatatga gtaagacgaa
    17101 gattacaaac tatgaaaatt atggcattaa acatattgct tttattttag atggtaatgg
    17161 tagatgggca aaggcgcgta aaagaccaag aacttatggt catgaaaaag gtttttccag
    17221 tctttttgat gttggtattg ctgttagtaa acgtaatatc ccctttatga gtgtatacgc
    17281 tttttctaca gaaaactggt cacgaccaaa aaaagaagtc gattttttat ttagaacact
    17341 cgaaaaaaga ttacatagtg tgttaaagaa agtacaaaaa cataatatta aaattgtgac
    17401 ttctggtgac ttaaaaagat tacctgaatc aacacaacaa gcgattaatt acgcaaaaga
    17461 agaatcaaaa aataatacgg gcaccacatt aaatatttgt ataaattatg gtggtaaaga
    17521 tgaaatcgtt cgtgcgatta aaagactagc agcagatgaa agtgttaata ttcaagcatt
    17581 aacacctgat acttttgaaa attatttaga tacaagcgga atgccgccgg ttgatctttt
    17641 agtgcggacg agtggcgaga tgcgccttag taattatatg ctgtggcaac tagcctacgc
    17701 tgaatttata tttaccccaa cttattggcc ggattatgac gaagagtgtc tcgtccgtga
    17761 tttagaagaa tacgcaaaaa gaacgcgcag gtttggagga ttatagtgat ggaaactaat
    17821 gaaattaaac caaaaaccaa acggacttca acgattaaaa tatcactaaa tgaaattagt
    17881 gaagcaacaa agaagtcaat gaagacccgc acaattactt cgattatttt agcggtaatc
    17941 gctgttcctg ccttgctttt aggtgggtgg tactttttag cgcttgtgtt ctttgtgtca
    18001 attattgctg tgcttgaaat tatgcgtggc acgaaaatat caaaacgcta ctggtgtgtg
    18061 tatatcttag cgattgtttc aactgttgcc cttatctttt ggatttttat taaaaataat
    18121 ataatggcta atattaaggc cggatactcg tggtgggata tttcaaagtg ggtggtggaa
    18181 aacggttttg aatcaattca agtatcaagt ttcttacttg cgaccatttt aattggtcta
    18241 tttatcttaa ttgtgtttga tccgcatctt ggttttgata tcgcggcgta tttattcttg
    18301 ttagtgctct ttgccgggtt tgggttccaa gctttcttat tcttaaggct ttatccgcaa
    18361 tatattgctg atcgtcctga cattactttt aacacgaata ttattacgag cgcattcctt
    18421 atcatttatg taattttagg aacaattatg aatgatattg gtgcttattt tatcggtgtg
    18481 ttatttggta aaaataagat ggtaccacgg atttccccaa ataaaacatg ggaaggcttt
    18541 gctggtggtg ttttcttttc gattgttgtt agttttttat tcgccttaat tgtgtcgtgg
    18601 attggttatc cggttttacc atttttgagt cataaagagt ggtattattt attactcatc
    18661 tcaattttaa tgccgcttgc ggcaaatatt ggtgacttat tctttagcgc taccaaaaga
    18721 catttagcga ttaaggatta tggttcgatt ttagttggtc atggcggtat tttagaccgt
    18781 gttgattcac ttttaatggt tagtattgtg acggcaatgc taatcatctt aataaataac
    18841 ggatggagta tactcgtcta gaaggaagtt agtatggaat ttttaagtac aatattaaac
    18901 attattttat ttttagtaat gcttggtgta ttaatcacca ttcacgaatt aggccacttt
    18961 attgctgcga agatatttag agtgtatgtc tttgaatttg cgattgggtt tggtcctaaa
    19021 attttccgca agaaaaaagg agaaacttat tattcattac gcgcattgcc gcttggggga
    19081 tatgttgcga tgctcggcga agaagatgct gtgcctgatg aatttgtaaa tgaagaaatt
    19141 cccaaagaac ggagtttaat cggcattaat cgtggcaaaa gggcattaat tatgtcagcg
    19201 ggtattatta tgaacttagt gttaggctac gctatattct tattcgctaa tggggtttta
    19261 ccgcaaaccg ggttatcaat tcgtttagaa gttaaagaag atagtcctgt atatgcaatg
    19321 gggatacgca gtgatgaccg cattgatttt aaagaaacga agattgatgg ttatacgatt
    19381 cagtacttcg gtgaagggaa gcttgacgct gcagcacttg aaccagaata ttatgtttta
    19441 tttcaaccaa agagtttaga tgacttaact tttggtggtg agtcgcttgt ccttttaaaa
    19501 attggcgcga ctgatttagc gagtgaaaat tcgttttaca ctgatttaaa agatacagat
    19561 gtgttaacct ttaccgcaac tttcttacgc ggtgaaactt atgaaactgc ggagcgtatt
    19621 gaaaaagtat taacacttag tactgaacca aaaaatcccg ataaattaac agacgggtat
    19681 aaatttgtgt ccgctggttt taatttaacg aaatttaaat atcgcaataa ctttagtgaa
    19741 acattcaccg ccgcaaataa agattttatc cgtagtgtta cggcagtagc gcgcggcctc
    19801 aagtcattat ttagcgatgg tcttaaaaat gttagtggac cagtggggat ttttaatatt
    19861 tctagtcaaa cacttaagaa tcatggttta gcaccttatt tattcttatg gggtttaatt
    19921 agtattaact tagcgctctt taacttatta ccgtttccac cacttgatgg atggcactta
    19981 ttagtggtaa cagttgaagc gattactcgt caagaaatta gtcctaaatt taaacaaatt
    20041 gcctcaatga ttggtgcaat tcttttgatt ggcttaacac ttgttatttt agcgaaagat
    20101 atttttggct tcgtaggagt tattttatag atgggaaacg ttagtgctcg tttttttgcg
    20161 tcaattaatt atgaaaacga caagaaaatt gggttaacgt ttttagaatg gaaaaaagtt
    20221 gatgaagtgt ggcactacac acttttaaaa gaaaaaccgt ggaattttga agactatcta
    20281 gattttagtg atgcgttaat gagtattcct tatgcatata aagtgagtta tgcttacact
    20341 aaattaccgc gtattgaaga tgctttagcg ctttttaaac cgtggcattt acatacttat
    20401 cttagtgaaa gcaactatgt tattatggtc gttggcaatg atacattacg ctttactttt
    20461 accgatgaag ttagtgcgct tgacgctaaa gcggtgcttt atgaattttc tttactctta
    20521 aaagaactat cttataactt cgccttagaa acagttgtca ttagtagtgc acaggaagaa
    20581 gacgaggaag acgaattttc acttgggagt gaaagaccct ttatcgaaga tgatgatgac
    20641 gatgaagaat attttgatga agaagatgac acgggtgtag cagattgcga tcctctagat
    20701 ccaaatgaaa actgtgaaag aagtcttgaa tatcacgacg ggttagaaga taaactaaaa
    20761 gaaaattacg aaaaaatggt taaagagcgg caaaaaagaa ggcacgctaa aggagctttt
    20821 aatcttaccg aaattaaaga tttagaacat aaagaagttg atgaaggtgt ggactttgac
    20881 ggtgaaatat atagttatga taggcgtccg acaaagcacg gtgtcactta tacactcggt
    20941 attggcaaag acgctcacgg tatttcagta cgattttata ttaataaacg tggcgcaaag
    21001 caagatgaag tgttaaaagc actaaagacg ggacgctttg tgcgtgtaca aggttatatc
    21061 gctattgata atttccgcgg tcaaactaaa attgttagag ggcgcgacat tgatgaaatt
    21121 gctgcaccac cattaccgac agatgattac gaaggtcgga aacgcatcga attacatcta
    21181 cacactaaaa tgtcagcgct tgatgccgta agtacgattg atgaatattg tgaacttgct
    21241 aaaaatatgg gccatgaagc gattgcactt actgatcacg gtgttgtgca agcctttcct
    21301 gaagcccaaa aagccgggaa gaaatatgat attaaaataa tttatggtag tgaactatat
    21361 gttatcgatg aaacattaaa agtcgttaat aaccctgcac caattatttt aaataatgcc
    21421 cgatatgtaa tttttgactt agaaacaaca gggctaagtg ctcgctataa cgaaattatt
    21481 gaatttggcg ctgtgcgtgt ggagcacggc ctagtgacag cgcggcttga tattttaatt
    21541 aatccaggta ttgtcgttcc tgaacatatt acacgcatta caaaaattac aaatgcgatg
    21601 ctgcgcggta aacctaaact agatgatgtc cttgatgaga ttttagagtt tttaggtgat
    21661 gacattttag tgtcacataa tgccgagttt gacttaggat ttttaaacgc taatttgcgt
    21721 aaaagaggac ggaacccgct taacaatcca acaattgaca ctttagcgtt atcgcgctat
    21781 ttattcccag aatcacgtgg tcaccgatta ggtgttttag cccgcaattt agaggtattt
    21841 tatgatgaag acggggcgca ccgtgcggat tacgatgcgg aagttttaaa tagtatttgg
    21901 caagcaatgc ttacaaaatt aacggaagac gatcaatatt taacacatgc cgcccttgcg
    21961 gagttaaaac taacacaagc tgcgctccaa tacttatggc cagcgcacgc tacggtgtat
    22021 gtgaaaaata aagacggact tcgtgattta tataaattag tgagtgtgtc tcataccgaa
    22081 ttctccaccg atattccaaa aacgccgcgc tccatgcttt taagccatcg tgataattta
    22141 ctaatcgggt caggatgttt taacggtgaa gtatttgaaa ctgcagcgcg ctttgatgaa
    22201 gaccgcttgc ttaaagtaat ggaattttat gattttattg aaattcaacc atacagcaat
    22261 tactcttact taattaatat gggtgaatta gatagtgaag agcatcttaa aaaagttatt
    22321 ggcgatatta aaagcgctgc gcaaaaatta gggaaaccgc ttgtggcaac gggtgatgtt
    22381 cactattgct taccgagtca aaaagttttc cgtgatgttt acattgtcgc aaaaggatta
    22441 aagaatgtta atcatccact taatccttat gatcgcgaca agcgtccaca ttttgaaaac
    22501 cctgatcaac attatcgttc tacaactgag atgtttgaag aaatgcgtaa gtttggtgta
    22561 tttaccgaag aagaattagc agatatgatt attaataatc cgcaccggat taatgattta
    22621 gtggaaaata ttacgccatt tacaaaagaa ttatataaac catcaattga aaatgttgat
    22681 gaacttttag aaaacttagt gtttgaaaca gcttatgaaa tttacggtaa tccattacca
    22741 actttaatta gtgaccgtat tcatcgtgaa ttaacaggga ttataaataa tggctatgca
    22801 gttatttact acttagcgca tctaattgtt aaaaaggcaa atgacgaagg atttattgtt
    22861 ggtagccgcg gtagtgttgg ctcaagcgtc gttgcttatt tcgctaaagt ctcagaagtt
    22921 aatccgctcc caccgcatta cttctgccct cattgtaaat ttagtaattt ccgtaatgaa
    22981 caaagagtac tctcgggtta tgacttacca ctcgctaaat gtcctgaatg cggacatgat
    23041 ttagttggtg aagggcaaaa tattcctttt gaaacattcc ttggttttga agctgataaa
    23101 attccggata ttgaccttaa cttccctgaa gactttcaaa aagaagcaca taacttcact
    23161 aaagaacttg ttggtgaaaa taatgctttt agagcaggaa cgattcaaac tgtggccgaa
    23221 cggacagcgt ttggtcatgt cttaaattat tacgaaaaac aagggatata tcgtgatgaa
    23281 ttaaataatg aaaatgttgc ttatcttgct tatcacatcg ctggtgttaa acgtacaaca
    23341 gggcaacacc cgggtgggat tgttgtaatt ccaagtgaat atgaagtata tgactttaca
    23401 cccgtacaat atccagcggg gaacacggaa agcgactggt taacaacgca ctttgactat
    23461 gattcaattc atgataactt attaaaacta gatttattag gtcacaaaga tccagtaaca
    23521 cttaagatgc tttgtgattt aaccggtatt aagtttgaag atgtgcccac taatgaccgt
    23581 gcaacaattt cgttattctc aagtcatgaa gcacttaact taaaaagtaa tccgcttggc
    23641 gaagtaacag gtgctttagg tttacctgaa tttggcacta actttgttcg tcaattatta
    23701 acggaagcaa aaccaaaaac atttaatgat cttgtgattg tttcaggttt aagccacgga
    23761 actggcgtgt ggcgctttaa cgctgatgaa ttaattaggt cagagcaagc aacacttgga
    23821 gatgttattg ggtgccgtga tgatattatg acatttttaa ttcgccaaaa tttacatgaa
    23881 aaagatgcct ttaacatcat ggaatcagtg cgaaggggac gcggtgttga agagggtctt
    23941 gaaacgaaga tgcgtgaagc tggtgttccg cactattaca ttgaatcatg taagaaaatt
    24001 gaatatctct tcccacgggc gcacgctgca gcttatgtta ttatggcagt gaaagtagct
    24061 tggtttaaac ttaattaccc gcttgagtat tatgcaacat tctttacaat ccgcggtgat
    24121 aactttgatc ttaaaacaat gataagtagc gaagaagtta tcttaaaaga gttgcaaaaa
    24181 tttgaggaac agcgtaaaac atcggaatta aatccgcgcg acagtaatat tgttgaaaac
    24241 ttacagttaa caattgagat gcttaatcgt ggctttaaaa tttcgaatat tgatctatat
    24301 aagagtgagg ctactcggtt taaagttgat catgaaaata atcaaatcat tccgcccttt
    24361 atcgtcatta gagcacttgg cgaaggcact gctgaaagcg tggttgaagc acggaagaat
    24421 ggtgaattta tttcgattga agatttagta gaaagaacac gtttaaacac ttcaaacatt
    24481 gaaaacttaa aagaactagg agcgctagag ggtctcccag aaagtaatca aattagctta
    24541 tttgacttta tgtaaattag tcttcaaaaa atttacttaa catcttgtta cacggaggcc
    24601 ttacgcctcc tttttcatag taagtcttta atttttctcc ggcaccaggt atttcaaggt
    24661 gatgagcaat tacaagataa taaaatgcaa tagcggtata gtcatcctta tcgtcacttg
    24721 cttcttcata atatttaaca agtggtaata gtgcctcata atttttaaag tcaacggcct
    24781 tttgcaacaa ttttccggca aaatcgttgt gttttttaaa taatgtcgct agttcatagt
    24841 acttttgacc taagaattta cggtggtctt gacctgactg tattgctgct agggataagt
    24901 gttctgttac tttgtcgata tttttttcga caagcgcact gcgtctagca tatacgaggt
    24961 taattatact aaccccgcta atacgtaatt tatattctaa gaaactatca aagttttcta
    25021 aatgcggata acctttttta tttgttaagt aaaagataat cttaataaaa tcatcgctta
    25081 atatttcaaa aggagcatct ttgtcattgc gattaaaatt aaaggcaaag gtaataaaat
    25141 tatgaagcgg ataattcgtg tgttttttta gtaacgctag cgtttcttcg cgctgcggga
    25201 catgaatcct agctccttca agtaaacgaa taacaatacc taaaaaggga tgcgataaat
    25261 aatcacggtc acgctgaaac ttaacccgaa tagcatcacc gacttcgata tttagatcga
    25321 cccagcgagc ttgtaaataa ttaataaaat caccgtttag tttacggaaa ccatcactat
    25381 atccttcata ggcattaagt gcatgtaaaa gattatttaa atactcgctt attatagttt
    25441 tccgataccc tgattcttta ttcatataaa tattttatca aattttaagg ttatcattgc
    25501 gtgtatagat aaaatcaaca gtgtttatga cacaaaagtt tggtaatttt taaaaacagc
    25561 gtataatatg tgtgcagtcg ggatgtagca cagcttggta gtgcgtctgg ttcgggacca
    25621 gaaggtcgca ggttcgaatc ctgtcattcc gaccattttt attttaaaga aatgatgttc
    25681 attgaaaaaa agtgtgacta atcacataat ttctcagcga aggttttaat gaatttagct
    25741 ttataaaaaa atttatgttg ttaatgtttt ataattatct tgtgcctccg tggtggaagt
    25801 ggtagacgcg atggactcaa aatccattag tagagatact gtatcggttc aagtccgatc
    25861 ggaggcacca aaaaattctg aaacaccggt tttaactatt aaaactgaga aataagtagc
    25921 tattgattaa tttcatggtt acttttttta ttttaagtat ttctttaaat gtacatatta
    25981 gaattttctt gtatcatttc ttgtatcgtt tcatgtatca tttcaaaatc aagtgtgata
    26041 agattatact aagaagtgga ggcatatgaa taaaacaaat ttaaaaaata aaataagcaa
    26101 aaggataatt attagcgaaa ttatttatgt tgtagttaca gcaataattt ctttgctcat
    26161 gttcttgcca atatataaag accgagctac tttgccagga tatgatgaag aaggtaacca
    26221 aattgttgtt aatttatttt atgaaaaaac tccttatcaa agactcaaag caatcaatat
    26281 cgagtggtta ttgtatttag gactttcact atttcttatt tgcatagtta tattaatcat
    26341 ttcttatacc actaatcata aactagataa atacaaaaaa gtgattttta taatatcatt
    26401 tggtcttatt cttgttttat tgctgattgc tgccatccaa ataacaatgt attgagatta
    26461 ttttctaaat atatagacca ttatattcac tttttaaagg caaaggagta aaacattgtg
    26521 ccatattgcg gtaaaccttt gtatggacaa cacaaaaaac atcgaaagat ttgattttat
    26581 gaatcagatc ttttttcttt ataaaaggac attgtaaatg attttcagca acccactaaa
    26641 aaacgggaac agccaaggct aaaaacacgg aataaaatta acaaaaaaac aggaataaaa
    26701 ttgacaaaaa tagatgtttg atgtatattt atattagagg tgatgtgaat atgaacttaa
    26761 aacctgaaaa atataaaaaa agaataattg atcaacaaat tgaaaagtat ctaaatattt
    26821 ttggagctgt ttgtgttgag ggcccaaaat ggtgcggaaa aacttggtca tctttaacac
    26881 attcaaaaag tgttatttat attggtgatc cagaaggtaa ttttcaaaat agaaccttag
    26941 cagaaattga tccaaaagga gtattaaatg gagattcacc tagattaatt gatgaatggc
    27001 aagaagttcc ttatttgtgg gatgcagtta gagccgaggt tgattttaga ggagaaaaag
    27061 gtcaatttat cttaacgggt tcatcaacac ctaagagaaa aggcgtgtta catagtggta
    27121 ttggaagaat tcatacttta agaatgaaaa caatgtctct ttatgagtct ttagattcat
    27181 caggtgaagt ttcattactt agtttatttg ataatgaaat taaaacgaac ttaacgaaac
    27241 aattttcatt aaaagaacta attgaattag ttgttagagg tggatggccg atgtctttag
    27301 aacaagataa agatttttat ggtgaaatcc ctaaatcata cgttaataca ttattaaata
    27361 cagatataca tgaaatcgat aatatttcta gaaataaaga taagtttgaa gcgttattaa
    27421 aatcactagc aagaaatgaa agtacacttg caggtaatcg agttttgatt aatgatatta
    27481 ataaatatga gaaagaaagt attgacgata aaactttagt ggaatacttg aatgtattag
    27541 aaaggttgtt tattgttgaa aatcaaaatg catttgaccc caattataga tctagtaaaa
    27601 gagttgcaag aactcctaaa aggcatttta ttgatccatc actagctgtt gctgctttag
    27661 gtatgtctaa agaaatgtta ttgaatgatt tagaatattt tggtttttta tttgaagctt
    27721 tagtaattag agatttaaaa atatatgcag aaagtattgg tgggaaagtt tatcattata
    27781 aacatcatga tacaaatcac gagttagatg ctgttgttga actaaatgat ggtaggtgga
    27841 ttgctattga agttaaatta ggagcaagtc aaattgatga tgccgcagaa aatcttttaa
    27901 aaattaacaa ctactttgtt aataatgaaa atacaagtaa gccgagtgca ttagtcgtaa
    27961 tatgcggttt gtcaaaggct tattataaaa gaaaagatgg tgtaatagta gtaccaatta
    28021 cggcacttaa gccataagag aagaggtaaa aaatgaaaat taataattgt ccatattgta
    28081 ataaagactt aaaaacgggt aaattagttt atcgttatgc atatagagca cctaaaatta
    28141 tttttaatga tggctcagaa aaaaagattg ataaacttaa actgtttaaa gatgccgtaa
    28201 ttaataaagt tttttattgt gaatcatgca atatcatgtt ggccgactta gatagcgatt
    28261 cacaaaaata gtttattaaa ataaaaacat ttaaatgata tttagtttta attggttgaa
    28321 aactacaaat tagggtctga acttttacaa aaatagggta taaatatagt aaggggtaaa
    28381 actttgtgcc atattggggt aaaactttgt acattatgtg attttagcgg gtaaaacttt
    28441 gtacggacaa cacatattga aacgtaaggt ttgatacaat ttcaaacctt ttttcttggc
    28501 taaaacacga taattgactt aatatcgtca tatttttatg tttgggacat ttgattttta
    28561 gatttgggtt gaaaaatgca tagttgcaca attttactcg ttaaggatac atagagggta
    28621 ttcactcgtt aattataata atcccactgg ttgtaaagat gtaaatgatg tgagacctaa
    28681 ttcttcaaaa aaagaagggt gatttgttaa tatc
//
LOCUS       DAOSTA010000012        27020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_1976_length_27020_cov_8.795735, whole genome shotgun sequence.
ACCESSION   DAOSTA010000012 DAOSTA010000000
VERSION     DAOSTA010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 27020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27020
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1976_length_27020_cov_8.795735"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..100
                     /locus_tag="PLU08_02105"
     tRNA            25..100
                     /locus_tag="PLU08_02105"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Asp,seq:gtc)
     gene            105..180
                     /locus_tag="PLU08_02110"
     tRNA            105..180
                     /locus_tag="PLU08_02110"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:138..140,aa:Phe,seq:gaa)
     gene            309..1094
                     /locus_tag="PLU08_02115"
     CDS             309..1094
                     /locus_tag="PLU08_02115"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HOQ70532.1"
                     /translation="MKLSIFNLICLIVIGVLLFLCKKFIKERRTHDLILKTTSILVIL
                     IHYSAVYVEFFQNQGVAYASASYILPVYPCHIMMWLLVIVAFTRHKKSRFFEGLAEFV
                     FVVGTLAALIGALFNMNFLNSSNPTFTNYFLFKGVFGHMVMFFGTTYLFVFEYVKPRV
                     EKTMISVIVGLLFFVADGIIINTLYAKFDIPPVNAMFLLEPPFEDYPFINFFTIGSIG
                     LILCFIGLLIYEHITLPKAEQWITKISTKSNKQDEDLTTDKTN"
     gene            1148..1948
                     /locus_tag="PLU08_02120"
     CDS             1148..1948
                     /locus_tag="PLU08_02120"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HOQ70533.1"
                     /translation="MFNLMHELFKDKDTGVFTFSAFSLCHIIYLLLIIGGIILVIFLY
                     KNKTQEAKTKLITTTVTIAFALYIADFFLMPFATGTIDIGKLPFHLCTLMSVMCFVVR
                     HNKKMEVFKTAFTLLGLIGGLMYITYPAGVAQADGYSYRIVQTVIYHGLMIAQGVFAL
                     VFDDLDLSWKNFKYDVIAVLAVAVWAYIGNTLYSGSNFWLSYEHDFNWFFVKHDGLYL
                     IDDEKDAFIAPLMSVSSVLAISALLRLIAIKLKSLFVKEQVLEAEKEA"
     gene            2064..4493
                     /locus_tag="PLU08_02125"
     CDS             2064..4493
                     /locus_tag="PLU08_02125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HOQ70534.1"
                     /translation="MNNKTKSRRLRFRNILLLWLVGLAGQLCWNLENQWFNTFVYAEV
                     GPFPQIVNAMVAVSAIATTFATFFFGTLSDRAGKRKPFISIGYIIWGIFTIAFGLTGL
                     LTKFTMNIWALATVIVLADTVMSFFGSMGNDAGFNAWTADLISDDNKGAIGTALAVQP
                     VIGTIIGTVLGGILIEAFGYIAFFSMMGGFVSLIGVLSLFLLKDSPKLEPSKRGSFWR
                     QFAEAFNFKYFFSQKELVLVNVVAAIYFIGFNVYFVHIGNLFISNYGFNAGDAGIIQG
                     IGLIIAILFTIPGSRLINRGKSPRVALISILMTIVGLIYLGLIGGYHDPANLFGAKNL
                     PLLLGVIVVGAGYILIMQTLGVWSKVLYPVGSKGQFEGLRIIFFVLIPMVLGSSISQP
                     IIRHFGIETVVEVLEDGTKVMGYIPTEALFYFASIIMVLALIPLIFLRKGYYKRMRNT
                     KISEAVSTAKEELGVDELYYTTPTPVFDKQKRHINFGYATDMSFVFDKKLVKKPLRLK
                     EWDFYQIQHGRYVVQLTLGHIAYAQNIAINLIDLEGEETYSASVVKLFKGAKSMTMPT
                     DPSVDNEIHYTDTKKKVDLHYVTKGRIRHLYGSMVNEKGVTFKVDVKLERLENHDAMV
                     INTPFEDSLYHFYLNYKIGNLRASGTFSMDDLAFTFDPKVDFGLLDWGRGVWPFHEVW
                     YWGQVNDYLADGRMINLNLGYGFGNLEAASENMMIIDGKAYKLGRLYIENDVTLDYMA
                     DWIIKSDDGKIDLVMKTIYDRYSDNDFKVIFMKCHQVYGRYYGTITLDDGTVVELTGQ
                     QGFFERSHNKW"
     gene            complement(4592..5098)
                     /locus_tag="PLU08_02130"
     CDS             complement(4592..5098)
                     /locus_tag="PLU08_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70535.1"
                     /translation="MKTLKLRVLNPRMHNVIYMFDGKALKPKGDNMGHYVFNIETPAD
                     KVDILIIRRSPLRSRLWLVWQFLFFIVSLLGILDLQSKKLNKEAIYRATLYLSGEDEV
                     DLKFDTDNSSNAFVELTTTLQVEERENKTLSDPLIVRRAKVLKILKIITYIVLLITLI
                     IILILIKK"
     gene            complement(5095..5601)
                     /locus_tag="PLU08_02135"
     CDS             complement(5095..5601)
                     /locus_tag="PLU08_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70536.1"
                     /translation="MQDTFNYNDYQNDQIVKKYDGRINRHHLGILFWRLGVAFSIIFI
                     VLLLARTLISITSIIIFMIGLVILSFIFLITLGTILIIDKNHGRHWKNLINILENRET
                     ALLVIDKILTFVPLICSVIIAFNIFALILLYTSKLPVRRFRLVTAIIIIILNIVALIY
                     ALAGGNFT"
     gene            complement(5747..6751)
                     /locus_tag="PLU08_02140"
     CDS             complement(5747..6751)
                     /locus_tag="PLU08_02140"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ70537.1"
                     /translation="MKFERTLAICLDMNGCPNRCLHCWLGDLPNHKLNDKTATKIVKL
                     FKPYFENITFYSWLREPDFTKDYKARWLKDNKLSTIKPKRFELASYFTIIRDPEYVKW
                     LKAVGTKKVQLTFFGLEAMTDKYVGRKGAYKELIAATKILQDNDIDVRWQLFINEENK
                     DEVSHLLDVAAENKVTDIFVHEGSCDGNNRKLYDIRINKDNIPLKVKPYYLNYENLLS
                     ESECIELLKDDDSYFIPKHKDEIVIYITSDLNVYFNFTNPSAAWQIGNIEDDDISEIV
                     RKIVAGDVPALKFARSILLSELVKLYGDSNSDKVFSLEDYKMYLLNNYVDDKYNSKLQ
                     "
     gene            complement(6925..7941)
                     /locus_tag="PLU08_02145"
     CDS             complement(6925..7941)
                     /locus_tag="PLU08_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70538.1"
                     /translation="MRKSKYKPLADYLSASNKNSVSLTFNQIEKILGFKLPPSSRKYR
                     ANWSNNKMEALAWGWLPVGYESFDVRMSDEIANFRKVGNKLKSEKSKKNNRKQNSDLK
                     RQIENATDLIFLDVPNNLLKLEVDEYYMVKLTKTNSEIVEHELANDISYPQKAKKICE
                     ELIKRNNDFSSETFNIIIHRIAIENSTRSSKKTIDLLTDFALNSENQFLQRVKKGDLT
                     LVDDITDYLFKNNNRRDKSLASKVCRYLNEWLFDKDDFTINDSVVRKVLPYYLAYYKI
                     EKHYWANKNLDKLTYVEFFAIFEKIKEKLPELTRHELDHLLWYSYKNDNIRSTIAASL
                     AKHL"
     gene            complement(8219..8581)
                     /locus_tag="PLU08_02150"
     CDS             complement(8219..8581)
                     /locus_tag="PLU08_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70539.1"
                     /translation="MEFKTVNYDSKIIKEGISHKGRRKSDDPVKIFSQQLKDQNVLYF
                     FYKDDECLYIGQTGICLWDRIIRHEDPEKDSKWFEEANKICLIILDKKVDVISRRNLE
                     STFIVNYLRAGHKLYNKE"
     gene            complement(9024..9098)
                     /locus_tag="PLU08_02155"
     tRNA            complement(9024..9098)
                     /locus_tag="PLU08_02155"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9064..9066),aa:Met,seq:cat)
     gene            complement(9151..9813)
                     /locus_tag="PLU08_02160"
     CDS             complement(9151..9813)
                     /locus_tag="PLU08_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOQ70540.1"
                     /translation="MPNYNELKGNNLNFSIEKNKPNILKGKTLLFLGASFTYGHASFG
                     ESFVEYIEVRNDCTCIKEAVSGTTLVEHIEDSYITRLKKVPLGKKYDALLCQLSSNDV
                     RLKQEFGVIKESDYDTKTICGAIQYIAKYARDVLKCPVIFYTCPYFDKERYQKLVSVL
                     NEIATKMQFSVIDMYNDKNFNNISAETYALYMADPVHPTKAGYYYWLTPYIESHLFAF
                     FH"
     gene            complement(9816..11087)
                     /locus_tag="PLU08_02165"
     CDS             complement(9816..11087)
                     /locus_tag="PLU08_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOQ70541.1"
                     /translation="MKIRFINALILTMEENRPLFYGEVHVEGNRITYVGPSGDFNEPF
                     DRIINAEGNLLMPGFKNAHTHSPMTFLRSYADDLPLYDWLHKAVFPLEDKLEPGDQYV
                     LSKIAFAEYLTSGVTACFDMYYSPLETAQAAIDVGFRAALVGTVTKYKESVCEMKAAY
                     HTINKMHPLVSYHLGFHAEYTSTPEILKELAKAAHELKVPIWSHISETETEVKECIER
                     HGYTPAVYFDKLGLYNYGGGGFHGVHFSDEDFAVYKKRGLSIVTNPSSNTKLASGIAP
                     INKFLKEGINVAIGTDGPASNNALDFFREMWLVTGLAKLREKDAAVVDAYDVLKMATV
                     NGAEAMGLKDAKYLSVGSLADIIMLDMQQPNMQPVNNIEKNIVYAGSKVNVLLTMIDG
                     KILYEDGEFNLDEPISEIYRKAQAITERLKS"
     gene            complement(11187..11309)
                     /locus_tag="PLU08_02170"
     CDS             complement(11187..11309)
                     /locus_tag="PLU08_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70542.1"
                     /translation="MVLGSEKKYLCLLCGMIIDNPDYCEYCGATSENIVPYEEE"
     gene            complement(11456..11659)
                     /locus_tag="PLU08_02175"
     CDS             complement(11456..11659)
                     /locus_tag="PLU08_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOQ70543.1"
                     /translation="MTRTYIVEGMKNERSVRRIERALFRVDNISYEINLEKNTVVIHF
                     SNRVDDDKVIGAISRAGYRAMRL"
     gene            complement(11662..13500)
                     /locus_tag="PLU08_02180"
     CDS             complement(11662..13500)
                     /locus_tag="PLU08_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ70544.1"
                     /translation="MSADVFREEEAVKKVTFGVWKQILKIILRSKKELSLLLIFAVFM
                     SLAEASTIPLNAYAISNLIETYNESALLPFIVLSVTQIVVFAFGVFGFIYFGSKLETK
                     VRYTLRKESFKNLQKLPFSYYDSTKQGWIMARMTSDTNRLARIVSWGILDLTWSGFFM
                     FFTIVVLFISEWRLALVFVSFLPILLFIAIIFRKKVLILNRKSRFHNSQLTGLFNESF
                     LGAKTTKSLAIESELSDEFTKEARVMKKVTLKAILYGAIFSSFLLAGSYTIVAFVMAL
                     GGYFALQGLVTMPILFMFIRAAMNTFEPILNLTNFINELQVAQASAERVLQLVNEKPS
                     IDDTQEVKDVYGDLFDKKKENWEEIKGDVEFKDVTFYYNPKEIILDKFNLKVKAGQTV
                     ALVGHTGSGKTTIVNLFARFYEPRAGEILIDGKDYRKRSLSWLHSRLGYVLQMPELFS
                     TSIKENIRYGNLEASDEEVIAASKAVGLDEFVNTLEKGYDTHVGEAGNLLSVGQKQLI
                     SFARAIIADPRILILDEATSSIDSEAEAKIQKATDELLKGRTSFVVAHRLSTIVNSDL
                     IVVLENGKIIEQGTHAELLGNRGHYFELYRNQFIAERREEKYLEID"
     gene            complement(13504..15330)
                     /locus_tag="PLU08_02185"
     CDS             complement(13504..15330)
                     /locus_tag="PLU08_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ70545.1"
                     /translation="MKRKKAKKHSILHLLKGHFWGMFFVTLLVIFHRVSYSFVPLFTQ
                     YILKYLQNNGVVTGVNLPLFVIEIFEKGQNVFEVSLYVALTLLLYQLFRFSLMVVEQR
                     LKGQVQESIALKLRVKLFDHIQSLSYHYHNNSDTGDLIQRVTTDVEVTTNFVVMRVMD
                     AVGLIFMVLSGAYQMYFLSPLLMYISLAVIPLYGISSYFYFKNIWKRYNDIEEKEAEI
                     LTVIQENVHNAKVVKAFANEKFETEKLEVKNKAHKNAVIKTNKIVGMYWAAMDFFALS
                     QYFVITLISVFLARNGQMDAGNAAAALMLVGLLIWPIRGLGRLINDVSHANVAYNRIT
                     TVLDEKSEFEINGDLLPEIAGKIEFKNVSFKFPGDSSYTLENLNFTIEKGQTVAIVGR
                     TGSGKTTITNLLMRMYEYEGSILIDDIELRDIEKHHLRKNMGTVLQNPFLYSRTIRDN
                     INITARKAPMEKIINASEIATIAHDIRDFEKGYDTMVGERGTTLSGGQKQRVAIARVL
                     ISEKPILIFDDALSAVDSRTDTLIRQALKQKEQTSTTLIITHRITTAKEADQIIVINN
                     KTVEDIGTHDTLAKAGGLYQVLWDIQGDLETEFLELVAKECV"
     gene            complement(15485..17332)
                     /locus_tag="PLU08_02190"
     CDS             complement(15485..17332)
                     /locus_tag="PLU08_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ70546.1"
                     /translation="MKKQSKPKHMKQTMRRLFSYLLKRPVPLVFIVLLVVWSGFTNGY
                     GIYVMREIIDKSILDFDINGLNKSLIKLAILFGSGALSALIYNPMMVKYSQTVIADIR
                     NELFAHSQKLPLSYFDRTPRGEIMNYFTNDVDTLNQALNTGFTTIIYSVVTGIGTIVF
                     LFLINFYLSLIVVFFIVVMFFMVLRNSQKMRVGFASVQKDTAHLNSYIEEMMLGLKVS
                     AMFSHQKEDMKEFKRRNDALTKSGIYANTLVLRKTPMIVSLSYFNYAISAIAGALFVL
                     NGWLSLGALGSYLIYVRQSSNPFNNFMTELNALYNGLSGAERIFAFLDLEQEIDEGKV
                     RLVSAVEIDGNIVETEHPTNSLAWKIPTANGEFTYEIFKGRVDFTNVVFGYKQDERVL
                     NNISLHAEKGEKVALVGSTGAGKTTIINLITRFYEIDSGTINYDGIDIRDIKKSDLRQ
                     SVSVVIQDTHLFTGTIFENIAYPSPGATIAEVERAAKIAHAHDFIVKLPQGYETMLYD
                     NGANLSSGQRQLIAIARAAILQPPVLILDEATSSIDTRSERLIEKAMDELMEDKTVFV
                     IAHRLSTIRNAKAILVMEKGSIIERGSHEDLILEGKVYYQLYTGQFEWT"
     gene            complement(17329..19059)
                     /locus_tag="PLU08_02195"
     CDS             complement(17329..19059)
                     /locus_tag="PLU08_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ70547.1"
                     /translation="MKKGLFKYFKVVWKEGILAIFFILFETVLETLVPFMASRVVDVG
                     IANNDLPYIYKWGALMVCSAFLAFFIGIISVRYVARTGQGFGAELRRAQFAKLQSLDQ
                     EARDKFAVSSLITRLTNDTYTIQNAIIIGMRGIFRAPFMITMVLMLSFNMNSKVAIVF
                     ALTLPVIAFAMLVIVFRVRPLYERMQRNFDELNRTLQENFQAIRVVKAYVTEEQEEAK
                     FKAINKMYRKVASNAFMTVSLNSPIMQLGLYSTIIGLLLVGGNLINTGEAKIGEIQGL
                     LTFVLQLLNAMLMLAQILIMFGRTSASKSRISEVLNTDTSLEYLINDLSVKAKPSLIV
                     KDVSFKYPGTSDDNYALKNINFTLKAGETLGIIGQTGSGKSTLVHLLARLYDVTDGEI
                     ILDDHNVKDYSADNLLDAINIVFQNNMLFSGTIRDNLLWGNKDASDEEINRALEASLS
                     DEIVNKLANGLNSEVGQAGSLLSGGQKQRIALARALLKRPKILVLDDSLSAIDTISEG
                     KIRTKLRKLYPELTLIIVAQRVSAIKDADHILVLHEGEIHGAGTHNSLVENDEIYHDI
                     YTTQLKGAKA"
     gene            complement(19103..19957)
                     /locus_tag="PLU08_02200"
     CDS             complement(19103..19957)
                     /locus_tag="PLU08_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOQ70548.1"
                     /translation="MKKIALVLGGGGAKGSYQIGVLKALHETKILKRVRVASGTSIGA
                     IHTLLLMHDLSIEEMINVWKEFTNEAIYGRNKFKETWNISGFYNTKGILEKILPFTNE
                     EAFRNTKIEGYVTLAKVPTKGLFAKLNMKNYEKEVVFLNESEHPLKAVLASAAIPLVF
                     GEKEIEGTRYVDGGLVDNFPFTPALIANANIIISIGLDPKLLPTKPLPGRLHIDFTPL
                     EELGPFPKVSLDFSPEKTQQYFDLGYENAKALIAYLKREKFFWFGGRLKLRKGRLVTL
                     ETLTAKTK"
     gene            complement(19961..20716)
                     /locus_tag="PLU08_02205"
     CDS             complement(19961..20716)
                     /locus_tag="PLU08_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70549.1"
                     /translation="MKTLANLLDNDTVTIVLFFAFLLLAVVIYLIVSKKEPSKEKMRP
                     EERALKMVEAPKTEVKTEVTPEKPVTEEVKTKKVEREAQKVKVKKEDTDDDQFAAIKY
                     SYSYTAQVSLAPEASRERYLKIKQEILSYTSVNNTISWREERFTLSGKTIVKFKLIGN
                     VLRMYIALTLEELSDVNIKIDDVSRYKEHELTPSLLRISGNTGVKRALQAIDILMQKL
                     ELKKDPQYKLGASDIPAAQTIDELLEAGLIRKI"
     gene            complement(20786..21496)
                     /locus_tag="PLU08_02210"
     CDS             complement(20786..21496)
                     /locus_tag="PLU08_02210"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOQ70550.1"
                     /translation="MVKQQNNLFLLNNLFNVRDLGEIDTKYGTKTRRYKYIRGTAKGT
                     MTEIEKEHFYNLGVRVVVDLRYTNEIEKTPSPLRGYKDIKYYHVDMMGEFWQMREQGY
                     KDMTDLYIDLLDDSQEKLLTIFKIFLKYPRDGIYFHCTAGKDRTGVVTMLLLDLVGVP
                     HSVIVENYSESYTNNLSRPGYKFIKPEWRRFILSEPEYMERTLDHLYTKYGGSRAYLS
                     HIGLRDDEINNLAATIVE"
     gene            21753..22529
                     /locus_tag="PLU08_02215"
     CDS             21753..22529
                     /locus_tag="PLU08_02215"
                     /inference="COORDINATES: protein motif:HMM:NF015159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOQ70551.1"
                     /translation="MKKIVIPLVMLLSLVGCKTEKLIRVATRGCIHEEILNAAKTSLK
                     EMGYRLNLETIDNALLANSSLSSGGIDANIIQRTDDLIAYNNRVSKRAELTSAGRVYL
                     SALGVYSGTKTTLEDYNPGDKIVISDRAEDYERIMSFIEEVFTVTVFTEQEKTPNYRQ
                     TPLDFLTIEYMNPDLIPEAQKHPEEAFYIMTGSQAYNSGLSYEHILATETSNDYAFIV
                     GIRRGEENNDRIRALMKVLYSDEIGDFIVEHFRGLIIPYR"
     gene            complement(22531..23205)
                     /locus_tag="PLU08_02220"
     CDS             complement(22531..23205)
                     /locus_tag="PLU08_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOQ70552.1"
                     /translation="MSPLEIVLVSVLGALILIVAIYLFVVHTNAVNRLEIFEEVNKEA
                     KSGKIAFLGDSLTDFFPIQDFFPGVKIYNRGIAGDTTKNVLKRLDNVVALAPRKLFLQ
                     IGTNDLAKGSSPRKIVANIKKIITTFKKEVPNIEVICISLYPVSRTKKFLSTFIVGLR
                     TNKKIKKTNELLINFCAEEGLKYLDFYTLLADKKGRIDRRYTVEGLHISGKGYAVIAK
                     ALAPYL"
     gene            23911..24885
                     /locus_tag="PLU08_02225"
     CDS             23911..24885
                     /locus_tag="PLU08_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70553.1"
                     /translation="MKNAICRIGELMAIAMGSLELVSMRIKEEPFDTSNTITRYTRET
                     SSTIRDSFFTTIDFKAAISDNTIIKGATLTADDSDMISLLNNDLYGIGYISYAALESS
                     ELKALIIEGVRVSEKSIIDDSYKLSRNFNYITKAPSDLSEKEYALITGFIAFSKSREG
                     LSIIKAKDGIVTQNIEEAESFTTLLAKSENSALAKMLYNTNEAPIHIKLGGSKSVESI
                     SRALSSAFTSLVPAFKAVHNYTSNEMAYEATQWSNKDSAEPLHIGFLARDLDINETEP
                     ALAGSYGVISKDAIVAVVSNKNHVLSDITRDQLKQIYKSETITWSALE"
     gene            complement(25005..25403)
                     /locus_tag="PLU08_02230"
     CDS             complement(25005..25403)
                     /locus_tag="PLU08_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HOQ70554.1"
                     /translation="MTPVHDAATSYAINFLYETRNCDATGAVNNISSHKREELGNLFN
                     ELAPDVQADLKNATATLSGDLVSQAMARYVYILQKYGTVTFTNFIDRTLPPSGIPAVR
                     GDNKQNFYVLGIMAFIAVAGFFFLNRKRRY"
     gene            complement(25410..26507)
                     /locus_tag="PLU08_02235"
     CDS             complement(25410..26507)
                     /locus_tag="PLU08_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOQ70555.1"
                     /translation="MEDLQFDTTPSLSRDYIYRYEDSPDANFITIYPGEIKVQVFNPD
                     NAGGSPAGARRMKVEYTSLLGATPSTPTGTSSYALRGPAVGDWTTHDINNCLVGISGY
                     DGRYLSLTRAFNADDFKIFVYKQTLNEPYQRGFIDFDDHKSNASDVIMPGDPSSGYHE
                     SFHVNTAGNYMVVYDLKMQKIYFHNAGTTYHKVTKYYGELLIEEAAAFTGLNYTPAPF
                     YQPEKKFVGWYEDALLETAYVPKDLTSDITLYAKFVDSPSVNLRFYDRNLDIKVDNGG
                     FNEYVVFAYGKNTDGTEPLGGWPGRMMQKVVDMGHELSYGYYALNISGQDRPEKVTFN
                     NGCQPFEPEHVSTPELVLPADKNLYEHDVAA"
     gene            complement(26610..26777)
                     /locus_tag="PLU08_02240"
     CDS             complement(26610..26777)
                     /locus_tag="PLU08_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70556.1"
                     /translation="MNKNRKWLARSAVISLLISAIITPAFFRKDAVKTKAATTIPVLV
                     GDSIGWEPSVS"
BASE COUNT         8481 a         5013 c         4312 g         9214 t
ORIGIN      
        1 gaactttatt atagtatata ggctggacca gtagtgtagt ggttaacatg tctgcctgtc
       61 acgcagaaga tcgcgggttc gagtcccgtc tggtccgcca gtaaggcccg atagctcaga
      121 tggtagagca gtggactgaa aatccacgtg tcgccggttc aattccggct tgggccacca
      181 ttattgcgcg aaaacggact tgaacaagtc cgtttttttc ttttattaca aattagtaat
      241 atttattaaa tttcatttga tataatgaaa tggttgtaat aggagatgaa tagtttatga
      301 cagctttcat gaaattatca atatttaatc tcatatgtct aatagtgatt ggagtattgc
      361 tttttttatg caagaaattc atcaaagaac gtcgaactca tgatcttatt ttaaaaacta
      421 cctcaatctt agttattctt attcactata gtgcagttta tgttgaattc tttcaaaatc
      481 aaggtgtagc atatgcatcc gcttcttata ttttaccggt atatccatgt cacattatga
      541 tgtggttgct cgtaatcgtt gcttttacgc gtcataaaaa gtcccgcttc tttgaaggac
      601 tagcagaatt tgtattcgtt gttggcactc ttgcggcttt aattggagca ctctttaata
      661 tgaatttttt gaatagtagt aatccaacat tcaccaacta ttttcttttt aaaggtgtat
      721 ttggtcatat ggtgatgttt tttggtacaa catacttatt tgtttttgag tatgtaaaac
      781 cacgcgttga aaaaacaatg attagtgtta tagttggact tctctttttt gtcgctgatg
      841 gtattattat taatacactt tatgcaaaat ttgatatccc acctgttaat gcgatgtttc
      901 ttttagaacc accgtttgaa gattatccgt ttattaattt cttcactatc ggttctattg
      961 ggctaatttt gtgctttatt ggactgctaa tttacgagca catcaccctg cccaaagctg
     1021 aacaatggat aactaaaata tcgactaaat caaataaaca agatgaggat ttaactactg
     1081 ataaaactaa ttaagtcatt tttagaaagg aataattatt cactactaat aattaaaaag
     1141 atattatatg tttaatctaa tgcatgaatt atttaaggat aaagatacgg gtgtctttac
     1201 tttctcggct tttagtttat gccatattat ttatttatta ttaattattg gtggaatcat
     1261 tttagtaatt ttcttataca aaaataaaac acaagaagca aagacaaaac ttattactac
     1321 cactgtgaca attgccttcg cactttatat tgctgacttt ttcctcatgc cttttgctac
     1381 cggcacaatt gatatcggta agttaccgtt tcacttatgt accttaatga gtgttatgtg
     1441 ttttgtagtg cggcacaata aaaaaatgga agtatttaaa acagccttta cccttcttgg
     1501 gctaatcggc ggattaatgt acattactta tcccgcaggt gtggcacaag ctgatggcta
     1561 tagttaccgt attgtgcaaa cagtaattta ccacggtctt atgatcgcac aaggggtttt
     1621 tgcgctagta tttgatgatt tagatttgag ttggaaaaac tttaaatatg atgttattgc
     1681 cgtcttagcg gtagcagtat gggcctatat tggtaatacg ctctatagcg gaagtaactt
     1741 ctggctttct tatgaacatg actttaattg gttttttgta aagcatgatg ggttatattt
     1801 aattgatgac gaaaaagatg catttattgc cccattaatg tcggtgtcat ctgtccttgc
     1861 tattagtgcc ttacttcgtt taattgctat taagcttaaa agcctctttg ttaaagagca
     1921 agttctcgaa gcagaaaaag aagcctaaaa ttcacttata tttcgtattt ttccgtttat
     1981 tagagagctt ttttatttat ttattaaagt agtttaacat ctagcaaaaa taagataaaa
     2041 tacatttaat aggagaaaaa attatgaata ataaaacgaa aagtcgtcgt ttgcgttttc
     2101 gcaacatttt actattgtgg cttgttggtc ttgctggaca attatgttgg aacttagaaa
     2161 accaatggtt taatacattt gtttacgctg aagttggtcc gtttccgcaa attgtcaatg
     2221 caatggtcgc tgtttcggcg attgctacaa cttttgccac attcttcttt gggacactta
     2281 gtgaccgggc tggtaaaagg aaaccattca tcagcattgg ctatatcatt tggggtatct
     2341 tcacgattgc ttttggttta actggtctac ttacaaaatt tacgatgaac atttgggcac
     2401 tagcgactgt tattgtcctt gccgatacag ttatgtcatt tttcggatca atgggaaatg
     2461 atgccgggtt taatgcctgg accgctgact taattagtga tgataataaa ggggcaatcg
     2521 gtaccgcact tgcggttcaa cccgtcatcg gcacgattat tggtacagta ctaggcggaa
     2581 ttttaattga agcttttggc tatattgcct tcttctcaat gatgggcggc tttgtgagct
     2641 taattggtgt cctttcgctt ttcttactta aagattcacc aaaattagag ccaagtaaac
     2701 gcggtagttt ttggcgtcaa ttcgccgaag cttttaactt taaatatttc tttagtcaaa
     2761 aagaacttgt actcgttaat gtcgtcgctg ctatttactt tattggtttt aatgtttatt
     2821 ttgtccatat tggtaattta tttatttcaa actatgggtt taacgctggg gatgctggta
     2881 ttattcaagg aatcggctta attattgcga tactctttac catacctggc agtcgcttaa
     2941 ttaatcgcgg caagagtccg cgcgttgcgt taatttcgat tttaatgacg attgttggcc
     3001 tcatttattt aggattaatt ggtggttatc atgatccagc gaatttattt ggcgccaaaa
     3061 atctcccgct tctactcggt gttattgttg ttggtgcagg ttatatttta attatgcaaa
     3121 cgctcggtgt ctggagcaaa gtgctctacc ctgttggtag taaaggacaa tttgaaggat
     3181 tacgcattat cttctttgtg ttaataccga tggtgttagg ttcaagtatt agtcaaccaa
     3241 tcattcgcca tttcgggatt gaaacagtcg ttgaagtttt agaagacggc acaaaagtga
     3301 tgggctatat tcccactgaa gcacttttct atttcgcttc tattattatg gtactagcgc
     3361 ttattccgct tatcttctta cgcaaaggat attataaacg gatgcgtaat acaaaaatta
     3421 gtgaagcagt cagtaccgct aaagaggaat taggtgttga tgaactatat tacacaactc
     3481 ctaccccagt atttgataaa caaaaacgac atattaattt cggttacgcg actgatatgt
     3541 catttgtatt tgataaaaaa ctagtgaaaa agccactccg ccttaaagag tgggacttct
     3601 atcaaattca acatgggcgt tatgttgtcc agctcacact tggccatatt gcatatgcac
     3661 aaaatatcgc gattaattta attgatttag aaggtgaaga aacttattcc gcaagtgtgg
     3721 ttaaactctt caagggtgct aagagcatga ctatgccaac tgacccatca gtcgataatg
     3781 aaattcatta caccgacacg aagaaaaaag ttgaccttca ttatgttacc aaagggcgaa
     3841 ttcgtcattt atacggctca atggttaatg aaaaaggtgt gacatttaaa gttgatgtta
     3901 aacttgaacg cttagagaat cacgatgcaa tggttattaa tacaccattt gaagattctt
     3961 tgtatcattt ctatctcaat tataaaattg gtaatttacg cgcaagtgga acgtttagca
     4021 tggatgactt agcgtttact tttgacccga aagttgactt tggcttactt gactggggtc
     4081 gtggggtgtg gccattccat gaagtatggt actggggtca agtaaatgac tacctcgcag
     4141 atggacgaat gattaatcta aacttaggct acggttttgg taatttagag gccgctagtg
     4201 aaaatatgat gattattgat ggtaaagctt ataagcttgg tcgcctctat attgaaaacg
     4261 atgtaacgct cgattacatg gctgactgga ttattaagtc cgatgatggt aagatagatt
     4321 tggtcatgaa aactatttat gaccgctata gtgataacga cttcaaagtt atctttatga
     4381 aatgccacca agtttatggt cgctactacg gtacaattac attagatgac ggtactgtgg
     4441 ttgaattaac tggccaacaa ggcttcttcg agcgctccca taacaagtgg taatttaatt
     4501 agtgtttcct gccataagtg ctgctcaata agtcctgcct aaatgaaaag tcacattgca
     4561 aaatgtggct ttttattttt ggatatttaa attatttttt aattaaaatt aagatgataa
     4621 ttagagtaat taaaagcaca atatatgtga ttatttttag aatttttagc actttagcgc
     4681 gccgcacaat aagtgggtcg ctaagtgttt tattttctct ttcttcaact tgtaaggttg
     4741 ttgttagttc cacaaaagcg ttactcgaat tatcagtgtc aaattttaag tcaacttcat
     4801 cttcaccact taaataaagt gtggctctat atattgcttc tttattaagt tttttactct
     4861 gtaaatccaa aataccaagg agtgaaacaa taaagaacaa aaattgccac actaaccata
     4921 atctgctgcg aagtggagag cggcgaataa taaggatatc gactttatcc gctggggttt
     4981 caatattaaa aacatagtgg cccatattat caccttttgg ctttagcgct ttaccgtcaa
     5041 acatataaat aacattatgc atacgtgggt taagcactct taactttaaa gtcttcatgt
     5101 aaagtttcct cccgctaagg cataaattaa tgcgacgata tttaaaataa taatgataat
     5161 agcagttact aaacgaaagc gacgcactgg taacttacta gtgtagagta agataagcgc
     5221 aaagatatta aaagcaatta ttacactaca tattagcgga acaaaagtta gtattttatc
     5281 aattactaaa agcgcagtct ccctgttttc gagtatatta attaagtttt tccagtgccg
     5341 accatgattt ttatcaataa ttaaaatcgt tccaagcgta attaaaaaga taaaagaaag
     5401 aatgacaagc ccgatcataa aaatgataat gctagtaatc gaaatgagtg ttcttgctaa
     5461 gagtaaaaca ataaagataa tactaaaagc aacacctaac cgccaaaata atatacctaa
     5521 atgatgacga ttaatacggc cgtcatattt ttttactatt tgatcgtttt gataatcgtt
     5581 gtaattaaaa gtgtcttgca atttcctttc tccttgcttt taaatgaagt atttttctaa
     5641 ttgtatctta aatttagact ttatcctaaa aatacctaat tgtaattaaa tagtctaact
     5701 aatgttttaa gtctaaaaaa ctggcaaaaa tgcactattt tgctctttat tgtaatttac
     5761 tattatattt atcatcaaca taattattga gtaaatacat tttataatct tcaagtgaaa
     5821 agactttatc ggagtttgaa tcaccgtata attttacaag ttcactaagc aaaatacttc
     5881 tggcaaattt taaagccgga acatcacctg cgacaatttt ccgcacaatt tcactaatgt
     5941 cgtcatcttc aatatttccg atttgccaag cagcactagg attagtgaaa ttaaaataaa
     6001 catttaaatc actcgttata taaatgacaa tttcatcttt gtgtttcggg ataaaatatg
     6061 aatcgtcatc ctttaaaagt tcaatacatt cactctcaga cagaagattt tcataattta
     6121 agtaataagg tttaacttta agaggaatat tgtctttatt aatacgaata tcataaagtt
     6181 tacgattatt accgtcacaa cttccttcat gcacaaatat gtcagtgacc ttattttctg
     6241 ccgctacatc taataaatgt gacacttcat ccttattttc ttcattgata aataattgcc
     6301 atcgtacatc gatatcgtta tcctgcaaga ttttagttgc agcgattaat tctttatatg
     6361 ctcccttacg tcctacatac ttatcagtca tagcttctaa accaaaaaaa gttaattgca
     6421 ctttttttgt gcccactgct tttaaccatt taacatattc aggatctctg ataattgtaa
     6481 agtagcttgc cagttcaaag cgttttggtt taatagttga aagtttatta tcttttaacc
     6541 atctagcttt ataatctttg gtgaaatctg gttcccttaa ccaactataa aaagtaatat
     6601 tttcaaaata tggtttaaaa agttttacaa ttttcgtcgc cgttttatca ttaagcttat
     6661 gattaggtaa gtcacctaac cagcaatgta ggcaacgatt tgggcagccg ttcatatcaa
     6721 gacaaattgc tagtgttctt tcaaatttca tctttaaatc ctcatttata taatttataa
     6781 cgctgattac gacttgttct tttaagcgga tattcacgaa tcactaaatt gtattttaac
     6841 gctggatcaa gataattttt acgcagtgtt tctttacttt ttagattgaa taatatttgt
     6901 atctgacttg ctttgtatta gaaattataa atgtttagca agcgaagcag caattgttga
     6961 gcgtatatta tcatttttat aagaatacca aagcaggtga tccaattcat ggcgggttag
     7021 ttcaggtaac ttttctttta ttttttcaaa aatagcaaaa aattcaacat aggttaattt
     7081 atcaagattc ttattagccc aatagtgctt ttcgatttta tagtaggcta aatagtacgg
     7141 tagaaccttg cgtacaacag aatcattgat agtgaagtca tctttatcga ataaccattc
     7201 atttagataa cggcaaactt tacttgctaa actcttatct cttctattat tatttttaaa
     7261 tagataatca gtgatatcat ctactaaagt taaatcaccc ttttttactc tttgtaaaaa
     7321 ttgattttca gaattaagtg cgaaatcagt taataaatct atcgtttttt tggagctcct
     7381 ggttgaattt tcaatcgcta ttctatgaat aataatgtta aatgtttcgc tggaaaaatc
     7441 attatttcgc ttgattagtt cttcacatat tttcttagct ttttgaggat aagaaatgtc
     7501 attagctaat tcatgctcaa caatctccga atttgttttt gttagtttta ccatgtaata
     7561 ttcatcaact tctaatttaa gtaaattatt tggaacatct agaaatatga gatctgttgc
     7621 attttcaatt tgtcgtttta ggtctgaatt ctgcttgcga ttattctttt tacttttttc
     7681 cgattttaat ttattaccaa ctttcctaaa gtttgcaatt tcgtcactca ttctcacatc
     7741 aaaggattca tatcctactg gaagccaacc ccatgccaat gcctccattt tattatttga
     7801 ccagttagca cgatattttc gagatgacgg cggcaattta aaacctagga ttttttctat
     7861 ttgattaaat gttaaactta cagagttttt attagatgct gacaaataat ctgccaatgg
     7921 tttatatttc gattttcgca tataacatca cttctctttg tccatattat ataacaaaaa
     7981 attaaggctt attttatact gagttttttt catttgttaa ctacttttaa atttatgctc
     8041 tttattttct gcttttacaa cttgagatta ttatttatta aaagacataa tctaatcttg
     8101 agaacctata aaaattacat ttaaacattt ataaatggca acattttgta attggcttga
     8161 ataaactctt tttatacaat agctaatact tttatattca aaagtcttaa aaatgaaatt
     8221 actctttatt atataatttg tggccagctc gtaaataatt aactatgaat gttgattcta
     8281 agtttcttct agatataaca tcaacttttt tatcaaggat aattaaacat attttatttg
     8341 cttcttcaaa ccattttgaa tctttttctg ggtcctcatg gcgaataatc ctatcccaaa
     8401 gacaaatgcc agtctgacca atatacaagc attcatcatc tttgtagaag aaatataaaa
     8461 cattttgatc ttttaattgt tgagaaaata tcttcacagg gtcatcactt ttgcgtcttc
     8521 ctttatgaga aatcccttct tttattattt ttgaatcata attaacagtt ttaaattcca
     8581 taattcctcc tttagattat ctctcaaatt agattgttcc tttttctgta aagcgatgat
     8641 ttaagtatga agtcgaacaa ccatgccttt acaaggtttt atcgatttac aattaaagaa
     8701 atttcatgtt cgtctacttc aaaatcaaga aaccgattgt aaagccgtaa tccgtgaaaa
     8761 tgttcctttg aatttttttc atatgtttta aaaattttat catatatatt ttcaattttg
     8821 ggcttatttc aaaaggtttt tgactttctt ttttaagaaa aagcactaaa ttttatttaa
     8881 taggcgaaca agctaacata ataaacatct cgtatttctt gctcagagtt aaaactttta
     8941 atatgttaag gcaaaaatat gtgttttgta cgaaaaaagg tttgatacat tgtttctatt
     9001 gtatcaaacc tttgctgtta acttggttgc ggagggagga tttgaacctc cgacctccgg
     9061 attatgggcc cgacgagcta ccactgctct actccgcgat gtattagtat tataacatga
     9121 ttattaatta atgcaaatta tctaattaat ttagtgaaaa aaggcaaata agtggctttc
     9181 tatgtaagga gtaagccaat aataatatcc cgctttagtt ggatgaacag gatcagccat
     9241 atataaagcg tatgtctccg cggaaatatt attgaagttt ttgtcattat acatatcaat
     9301 gacactaaac tgcattttcg tagcaatttc gttaagtaca cttactaatt tttgataccg
     9361 ctccttatca aaataaggac aagtgtaaaa aataacagga cacttaagca catcacgagc
     9421 gtactttgca atatattgaa tagcgccaca tattgttttc gtatcgtaat cactttcttt
     9481 aataacacca aattcttgtt taagccgcac atcattactt gagagctgac aaagtaatgc
     9541 gtcatacttc ttgcctaacg gtactttttt tagcctagta atataactat cttcaatatg
     9601 ctccacaagt gttgtgccgc tcacggcttc tttaatacaa gtacaatcat tccgcacttc
     9661 aatatattcg acaaacgatt caccaaacga agcgtgacca taagtaaaag acgcaccaag
     9721 aaataataat gttttacctt ttaaaatatt tggcttattt ttctcaatac taaaatttaa
     9781 attattgcct ttaagttcat tataatttgg catatttaac tttttaaccg ttcagtaata
     9841 gcttgcgctt tacgataaat ttcactaata ggttcatcaa gattaaattc accgtcttca
     9901 tataaaatct tgccatcaat catcgttaat aatacattaa ccttactacc agcgtacaca
     9961 atattttttt caatattatt aacaggttgc atattcggtt gctgcatatc aagcataatg
    10021 atgtccgcta gactgccaac acttaaatat ttagcgtctt ttaatcccat tgcttcagca
    10081 ccatttacag tagccatttt aaggacatca taagcatcaa caaccgccgc gtctttttca
    10141 cgtaacttcg ctaaaccagt aacaagccac atttcacgga agaaatcaag cgcgttatta
    10201 ctagcgggcc catcagtgcc aattgcaaca ttaatccctt ctttaaggaa tttgttaatc
    10261 ggggcaattc cgcttgcaag tttagtattt gaactaggat tggtcacaat acttaaaccg
    10321 cgctttttat acacggcaaa gtcttcatca ctaaagtgca ctccgtggaa accgccacca
    10381 ccgtaattat ataaacctaa tttatcaaaa tacactgctg gggtgtagcc gtggcgttca
    10441 atacattctt ttacttccgt ttctgtttca ctaatatgac tccaaattgg gacttttaat
    10501 tcgtgagcag ctttcgctaa ttcttttaat atttcgggcg tgcttgtata ttcagcgtgg
    10561 aacccaagat gataactaac aagcggatgc attttattaa tcgtgtgata tgctgctttc
    10621 atttcgcaaa cactttcttt gtattttgta acagtgccca caagtgccgc tctaaaacca
    10681 acatcaattg ccgcttgcgc tgtttcaagt ggtgaataat acatatcaaa acatgccgtc
    10741 acgccacttg ttaagtattc cgcaaaagca attttactta agacatattg atcgccaggc
    10801 tcaagtttat cttcaagcgg aaatacggcc ttatgtaacc agtcataaag cggtaagtca
    10861 tcagcgtaac tccgtaaaaa tgtcatcggt gaatgcgtat gggcgttttt aaagccaggc
    10921 atgagtaaat taccttcagc gttaataata cggtcaaacg gttcattaaa gtcaccactt
    10981 ggcccaacat aagttattct attaccttca acatggactt caccataaaa aagcggacga
    11041 ttttcttcca ttgttagaat aagtgcgtta ataaaacgta ttttcataat gttttcctca
    11101 tttattagat acttaatttt agcatgtttt tattaataat aaaaaacatg ggttgcccca
    11161 tgctttttta tatgtttatt taatatttat tcttcttcgt aaggaacgat gttttctgat
    11221 gttgcaccac agtattcaca ataatctggg ttatcaataa tcatcccaca taataaacat
    11281 aaatatttct tttcagaacc taataccata tttatttcct cttcctccca taccctatta
    11341 tatcttataa ttattagaag taaacccgct gatgcattaa tttacaacta attttcttta
    11401 tttataaatg tgtttaaatc aagtttttta ttacaaaatt ttaaattttc attaattata
    11461 atcgcattgc ccgataccca gcgcgactaa ttgcaccgat aactttgtca tcatctactc
    11521 tattactaaa gtggatcaca acagtatttt tttctaaatt tatttcgtaa cttatattat
    11581 ccactctaaa taacgcgcgt tcaatacgac gtacggatcg ttcgtttttc atcccttcaa
    11641 caatataagt ccgtgtcact attaatcaat ctccagatat ttttcttcac ggcgttccgc
    11701 gataaattga tttcgatata attcaaagta atgcccgcga ttacctagta attcagcgtg
    11761 agtaccttgc tcaataatct taccgttttc taaaacaacg attaaatcac tattaacaat
    11821 cgtgcttaag cggtgcgcaa caacaaaact tgttcggcct tttaagagtt catcagtggc
    11881 tttttggatt tttgcttcag cttcgctatc aatcgaacta gtcgcttcgt caagaattaa
    11941 aattcgtgga tcagcaataa ttgctcgcgc aaaagagatg agttgctttt ggcccactga
    12001 taaaaggtta ccagcttcac caacatgcgt gtcgtatccc ttttctaagg tattcacaaa
    12061 ttcatcaagg ccgactgctt tactcgcagc aatgacttct tcatcacttg cctctaaatt
    12121 accatagcga atgttttctt taatcgatgt ggagaaaagc tcgggcattt gtaaaacata
    12181 tcctaagcgg ctatggagcc atgatagact acgctttcga taatctttac catcaattaa
    12241 tatttcacca gcgcgcggct cgtagaatct tgcgaaaaga ttaacaatcg ttgttttccc
    12301 gctgccggta tgaccaacaa gtgctactgt ttgcccagct ttaactttta aattaaattt
    12361 atcgagaata atttcttttg gattgtaata gaatgtcaca tctttaaatt caacatcacc
    12421 tttaatttct tcccaatttt cttttttctt atcaaataaa tcaccataca catctttaac
    12481 ttcttgcgta tcatcaatcg acggcttttc gttgactaat tgtaaaacac gttcagcact
    12541 agcttgcgcg acttgtagtt cattaataaa gttcgttaag tttaagatag gttcaaaagt
    12601 attcattgcc gcacgaataa acataaagag aatcggcatc gtcactaagc cttggagggc
    12661 aaagtaccca ccaagcgcca tgacaaaggc aacaattgtg taactgcccg ctaaaaggaa
    12721 actcgagaaa atagcgccat aaagaatcgc ctttaacgtc acttttttca ttacgcgcgc
    12781 ttctttcgta aattcgtcac ttaattcact ttcaattgct aagctcttag ttgttttagc
    12841 gcctaaaaat gattcattaa aaaggccagt taactgacta ttatggaaac gtgatttacg
    12901 atttaagatt aaaactttct tacggaaaat aatcgcaata aaaagtaaga ttggtaaaaa
    12961 tgacacaaac actaaagcta gccgccattc actaataaat aagacaacaa tcgtgaaaaa
    13021 cataaagaaa cctgaccaag ttaaatctaa gattccccat gagacaatgc gcgctaaacg
    13081 gtttgtatca cttgtcatcc tggccataat ccagccctgt tttgtcgagt cataatagct
    13141 aaatggtaat ttttgtaaat ttttaaatga ctctttgcgt aatgtatatc gtacttttgt
    13201 ttcaagtttt gaaccaaagt aaataaagcc aaagacacca aaggcaaaga caacaatttg
    13261 ggtaacactt aatacaataa aggggagtaa ggcactttca ttatatgttt caataagatt
    13321 actaattgca taagcattaa gcggaattgt tgaagcttcc gcgagtgaca taaacaccgc
    13381 aaaaattaag agtaacgata gttctttttt acttcttaaa ataatcttta agatttgttt
    13441 ccaaactccg aaagttactt ttttaactgc ttcttcttcg cgaaaaacat ctgcactcat
    13501 actctaaaca cactccttag caacaagttc taagaactct gtttctaaat ctccttgaat
    13561 atcccataag acttgatata acccgcccgc tttcgctagg gtgtcatggg ttccaatatc
    13621 ttcaacagtc ttgttattaa ttacaataat ttgatcagcc tctttagcgg tagttatccg
    13681 gtgagtaata ataagcgttg tcgatgtttg ttctttttgt tttaatgctt gacgaataag
    13741 ggtatcggtg cgactatcaa ccgcgcttaa agcatcatca aatattaaaa ttggtttttc
    13801 actaataagc acgcgcgcaa tcgctacccg ctgtttttgc ccgccagaaa gcgttgttcc
    13861 tctttcgcca accatcgtat cataaccttt ttcaaaatca cgaatatcat gggcaattgt
    13921 ggcaatttca ctggcattaa taatcttttc catcggggcc ttacgcgcag taatattaat
    13981 attgtcacga atagtgcggg agtaaagaaa cggattttgt aaaaccgtac ccatattttt
    14041 ccgtaagtga tgtttttcaa tgtcgcgaag ttcaatatca tcaattaaaa ttgacccttc
    14101 atattcatac atccgcatta ataaattagt aatcgttgtt ttcccgctgc cagtgcgccc
    14161 aacgatcgct acagtttggc ccttctcaat tgtaaagttc aagttttcta aggtataact
    14221 actatcacca ggaaacttaa aggacacatt tttaaattca atttttcccg caatctccgg
    14281 taataaatca ccattaattt caaattcact cttttcatcg agtactgtcg taatacggtt
    14341 atatgctaca ttagcgtgcg agacatcatt aattaatcgg cctaaaccac gaattggcca
    14401 gattaaaagg ccaacaagca ttaacgctgc ggctgcgtta ccagcatcca tttgtccgtt
    14461 tctagcaaga aacacactaa tgagggtaat tacaaaatat tgactaagag caaagaaatc
    14521 cattgctgcc caatacatac caacaatttt attagtttta atcactgcat ttttatgtgc
    14581 tttattttta acttctaact tttctgtttc aaacttttca ttagcaaaag ccttcacaac
    14641 tttagcgtta tggacatttt cttgaataac ggttaaaatt tccgcttctt tttcttcaat
    14701 atcgttataa cgcttccaaa tatttttaaa atagaaataa gaagaaatac cataaagcgg
    14761 aatgaccgcc agcgaaatat acattaaaag cggacttaaa aaatacattt gatacgcccc
    14821 gcttaacacc atgaatatta aaccgaccgc atccattacg cgcattacga caaagtttgt
    14881 tgtcacttcc acatcagtcg ttactctttg aatgaggtca ccagtgtcac tattattatg
    14941 ataatgatag ctaagtgact gaatatgatc aaaaagttta acgcgtaatt ttaaagcaat
    15001 gctctcttgc acttggcctt taagtcgttg ctcaacaacc attaaactaa aacgaaataa
    15061 ttgatataaa aggagcgtaa gggcaacata aagtgaaacc tcaaaaacat tttgaccctt
    15121 ttcaaatatt tcaataacaa ataacggtaa attaacaccg gtcactactc cattattttg
    15181 taagtatttt aaaatgtact gtgtaaatag tggtacaaag gaatacgata cacgatggaa
    15241 gataactaaa agcgtaacaa aaaacatccc ccaaaagtgg ccttttagta agtgtaagat
    15301 tgaatgtttc tttgcttttt ttctcttcat atctatcctt cttttaatag attctattaa
    15361 aacaatctct actattatac attaaatgga ataatttata acaacgataa aattaaaaaa
    15421 ggtacatatt tacatacttt acacaaattg tacctagtaa agtgctttca taaggaagtt
    15481 tattctatgt ccattcaaat tgaccagtgt ataactgata gtaaacttta ccttctaaaa
    15541 ttaaatcctc atgacttccg cgttcaataa ttgagccttt ttccattact aatattgctt
    15601 tagcgtttcg aatagtgctc aaacggtgag caataacaaa taccgtttta tcttccatta
    15661 actcatccat cgctttttca atgagtcttt cactacgggt atcaattgag ctagtagctt
    15721 catcaaggat taagactggt ggttgtaaaa tagcagcgcg cgcaatagca ataagttgcc
    15781 gctgtccact tgaaagattt gccccgttat cgtaaagcat cgtttcataa ccttgtggta
    15841 atttaacaat aaaatcatga gcgtgcgcaa ttttagcggc gcgttctact tcggcaattg
    15901 ttgcgccagg actaggataa gcaatatttt caaaaatagt accagtaaat aaatgtgtat
    15961 cttgaataac gacacttact gattggcgta aatcactttt tttaatatcg cgaatatcaa
    16021 tcccatcata attaatcgtg ccgctatcaa tttcataaaa gcgagtaata agattgataa
    16081 ttgttgtttt accggcacca gttgaaccaa caagcgcaac tttttcacct ttttcggcat
    16141 gtaaactaat attatttaag acgcgttcat cttgcttata accaaaaaca acattagtaa
    16201 aatcgactcg ccctttaaat atttcatagg tgaattcccc gttagccgtc ggaatcttcc
    16261 acgctaaact attagtcggg tgttcagttt caacgatatt accatcaatt tcaacagcgc
    16321 tgacaagccg gactttacct tcatctattt cttgttctaa gtcaagaaaa gcaaaaatcc
    16381 gttctgcccc tgaaagcccg ttatagagag catttaattc agtcataaag ttattaaacg
    16441 gatttgaact ttggcggaca taaattaagt atgatcctag tgctcctaaa cttagccagc
    16501 catttaatac aaaaagcgca ccagcaatcg cgctaatcgc ataattaaaa tacgacaaac
    16561 taacaatcat tggtgtcttt cgtaacacta aagtattcgc gtatattccg cttttcgtta
    16621 atgcatcatt gcgccgctta aattctttca tgtcttcttt ttggtgtgaa aacattgctg
    16681 atactttaag ccctaacatc atttcttcaa tataactatt taaatgcgca gtatcttttt
    16741 gaacactagc aaaaccaacc cgcatttttt gactattacg cagtaccata aaaaacatta
    16801 caacgatgaa aaagacaaca atcagtgaca aataaaaatt aataaggaaa agaaatacaa
    16861 ttgtgccaat gcctgttact actgaataaa ttatcgttgt aaacccagta ttaagtgctt
    16921 gattaagtgt gtcaacatca ttagtgaaat agttcataat ttcaccgcgc ggtgtcctgt
    16981 caaaataaga tagcggtaat ttttgactat gagcgaaaag ttcattgcga atgtcggcga
    17041 taacagtttg tgaatattta accatcatcg ggttataaat aagtgcactt aaagcacctg
    17101 aaccgaaaag gatcgctaat ttaataagtg acttatttaa accatttata tcaaagtcta
    17161 atatgctttt atcgatgatt tcacgcatta cataaatacc gtaaccatta gtaaacccgc
    17221 tccacacaac aaggagtaca ataaaaacta gcggcacagg tcgttttaat aaataactaa
    17281 agagccggcg cattgtttgc ttcatatgtt taggcttact ttgctttttc atgcttttgc
    17341 tccttttagt tgggtcgtgt aaatatcgtg ataaatctca tcattttcca caagtgagtt
    17401 atgcgtacct gccccgtgaa tttccccttc atgcaacact aaaatatgat cagcgtcttt
    17461 aatagcactc acacgttgcg caacaataat taaagtaagt tccggataaa gtttccgtaa
    17521 tttagtgcga attttccctt cactaatcgt atcaatcgct gataaagaat catcaagcac
    17581 taatattttt ggtcttttta gtaatgctct tgctaatgct attcgttgtt tttgaccacc
    17641 tgaaagcaaa gagcctgctt gcccgacttc actatttaaa ccatttgcta atttattcac
    17701 aatttcgtca cttagacttg cttctaatgc ccgattaatt tcttcgtcac ttgcatcttt
    17761 attaccccat agcaagttat cacgaattgt tccgctaaag agcatattat tttgaaagac
    17821 aatgttaata gcatctagaa gattatccgc actataatct ttaacattat ggtcgtctaa
    17881 aataatctca ccatcagtta catcataaag ccttgctaaa agatggacga gtgttgattt
    17941 accgcttcca gtttgaccaa taatcccaag tgtttcacca gccttaagcg tgaaattaat
    18001 gttttttaaa gcataattat catcacttgt gcccggatac ttaaaactta catctttcac
    18061 aattaaactc ggttttgctt taacacttaa atcattaatc aagtattcaa gactagtgtc
    18121 agtgttaagt acttcactaa ttctactttt tgaagcgcta gtgcgaccaa acataattaa
    18181 tatttgcgca agcatcagca tcgcatttaa gagttgtaaa acaaaggtta gtaacccttg
    18241 aatttcacca attttagctt cgccagtatt aattaaatta ccaccgacaa gcagaagacc
    18301 aataatcgtt gaataaagtc caagttgcat aatcggacta ttgagtgata ctgtcataaa
    18361 cgcattacta gcgactttac gatacatctt attaatcgcc ttaaactttg cttcttcttg
    18421 ctcttccgta acataagctt taacaacacg gatcgcttga aagttttctt gtaaagtacg
    18481 gtttaattca tcaaaattac gttgcatccg ttcataaagt ggtctaactc gaaaaacaat
    18541 gactagcatc gcaaacgcaa taacaggaag cgtaagtgca aatacaattg ctactttaga
    18601 attcatattg aaactaagca ttaataccat cgtaatcata aatggagcac ggaatatccc
    18661 gcgcatccca ataatgatgg cattttgaat agtgtaagta tcatttgtaa gccgagtaat
    18721 taaggaagac actgcgaatt tatcgcgcgc ttcttggtct aaactttgta gtttcgcaaa
    18781 ttgagcacga cgtaattccg ccccaaatcc ttgaccagta cgagcgacat aacggacact
    18841 aatgatgcca ataaagaaag caagaaaagc cgaacatacc ataagggcac cccatttata
    18901 gatataaggt aaatcattat tagcgatacc aacatcaaca acgcgacttg ccataaatgg
    18961 cactagtgtt tctaaaacag tttcaaataa aataaaaaag atggcaagaa tcccttcttt
    19021 ccatactact ttaaaatatt tgaataatcc ttttttcata gcattattat gccagtaaaa
    19081 tctctttttc tctatatata agctacttag tttttgctgt taatgtttct aacgttacta
    19141 ggcgtccttt tcttagcttt agtcttccac caaaccagaa aaacttttcg cgttttaagt
    19201 acgctatcaa cgccttagca ttctcatacc ctaaatcaaa atattgctgt gttttttcag
    19261 gactaaaatc aagcgacact ttaggaaatg gtcctaactc ttctagcggg gtaaaatcaa
    19321 tatgtaagcg tccgggtaat ggttttgttg gtaaaagttt tggatctaga ccaattgaaa
    19381 taattatatt agcgttagcg ataagcgctg gtgtaaacgg gaaattatcg actaaaccgc
    19441 catccacata acgagtgcct tcaatttctt tttccccaaa gacaagcgga atagcagcac
    19501 ttgctaaaac agcttttagc gggtgttcac tctcatttaa aaagacaact tctttttcgt
    19561 aatttttcat atttaatttt gcaaataaac ctttagtggg tacttttgct aaagtgacat
    19621 agccttcaat ctttgtattt ctaaatgctt cttcattagt gaacggtaat attttttcta
    19681 agataccttt agtgttataa aagccgctta tattccatgt ttctttgaat ttgtttctac
    19741 cataaatcgc ttcatttgtg aattctttcc acacattaat catctcttca atacttaaat
    19801 catgcattaa taaaagggtg tggatggccc caatcgaggt accgctagcg accctaacgc
    19861 gttttaatat tttagtctcg tgcagcgctt ttaatacacc aatttgatag gagcctttcg
    19921 cgccaccacc acctaaaact aaggctatct tcttcataaa ttaaattttc cgaataagtc
    19981 cagcttctaa gagttcatcg attgtttgcg ctgctggtat atcacttgcc cctagtttat
    20041 attgtgggtc tttttttagt tcaagtttct gcattaaaat atcaatcgct tggagcgcac
    20101 gcttgacacc agtattacca ctgatacgta ataaacttgg tgttaattcg tgttctttat
    20161 aacgactaac atcatcaatc ttaatattta catcacttaa ttcttcaagc gttaaggcaa
    20221 tatacatccg taaaacatta ccaatcaatt taaatttaac aattgttttc ccgcttaatg
    20281 taaagcgttc ttcacgccat gaaatagtat tattaacact agtgtaagag agaatttctt
    20341 gttttatttt taaatagcgt tcgcgacttg cttctggcgc taaactaact tgtgcagtat
    20401 aagaataact atatttaatg gcggcgaatt gatcgtcatc agtgtcttct tttttaactt
    20461 taactttttg tgcttctctt tctacttttt ttgttttaac ttcttcagta accggttttt
    20521 ctggcgttac ttctgtttta acttctgttt taggcgcttc aaccattttt agcgctcttt
    20581 cttcaggccg cattttttct ttacttggtt cttttttgga aacaataaga taaataacga
    20641 ccgctaagag taggaaagca aaaaataata caatcgttac tgtatcatta tcgagtaagt
    20701 ttgctaaggt tttcatatgt atctcctttt gcttatagcg gtgtttacac aataattgta
    20761 cttattttag gtgctttttg caatgttatt cgacaattgt ggccgcgaga ttattaattt
    20821 catcatcacg taagccaata tgcgataagt aagctctgct cccaccatac tttgtgtata
    20881 aatgatctaa tgttcgttcc atgtattcag gttcactaag aatgaagcgt cgccattctg
    20941 gtttaataaa tttatagcct ggccgcgata agttattagt gtacgattca ctataatttt
    21001 caacgattac agaatgcgga acacccacta agtctaggag taacattgtg acaactccgg
    21061 tccgatcttt accagcggta cagtggaaat aaataccgtc ccgcggatac tttaagaaga
    21121 ttttaaagat cgttagcagt ttctcttgcg agtcatcaag caaatcaatg tataagtcgg
    21181 tcatatcttt ataaccttgt tcacgcattt gccaaaattc gcccatcata tcgacatgat
    21241 aatacttgat atctttataa cctcgtaacg gacttggtgt tttttcgatt tcattcgtat
    21301 agcgtaaatc cactactact cttactccta agttataaaa gtgctctttt tcgatttcag
    21361 tcattgtgcc tttggcagta cctctaatat atttataacg ccgtgtctta gtaccatact
    21421 ttgtatcaat ctcacctaag tcacgcacat tgaataagtt gtttaagaga aataaattat
    21481 tttgttgttt gaccataact acctcctgct tacactatga tatcacatta cactacttct
    21541 ttctttagta aagaaaacgc attatttact aagaaaagtc gctaaattgg ctaaaaaatt
    21601 gttatcgtta tcattttttt cgtattttta ggtgtttttg gttgtaattc cctgcaaata
    21661 tgctttaatt aagatgaaat accttttaat aaaggtagat tataagcgaa agtaatcatt
    21721 aaaatgtatc tcgcacacta accttaaagc ttatgaaaaa aattgttatc ccgttagtaa
    21781 tgctcttatc cttagtcggt tgtaagactg agaaactaat tcgtgtcgca acgagaggtt
    21841 gcattcatga agagatttta aacgcggcta aaacttcact taaggaaatg ggttatcgct
    21901 taaatcttga gacaattgat aatgctttgc tcgctaattc ttcattatca agtggcggga
    21961 ttgacgcaaa tattattcaa agaacagatg atttaattgc ctataataac cgggtatcaa
    22021 aacgcgcaga gcttactagt gctggtcgcg tctatctttc agcacttggt gtatattcag
    22081 gaacgaaaac aacactcgag gattataatc ctggcgacaa aattgtcatt agtgatcgcg
    22141 ctgaagatta tgagcggatt atgagtttta tcgaggaagt ttttactgtt actgtattta
    22201 ccgaacaaga aaaaacacca aattacagac aaacaccact tgactttttg accattgaat
    22261 atatgaatcc agatttaatt ccggaagccc aaaagcatcc tgaagaagct ttttacatta
    22321 tgactggttc acaagcttat aacagcggac ttagttatga acatattctc gctactgaaa
    22381 ctagtaatga ttatgcattt attgtcggta ttcgtcgcgg cgaagaaaat aatgatagaa
    22441 tacgcgcact aatgaaagta ttatatagtg atgaaatcgg tgactttatc gttgaacatt
    22501 tccgcggatt aattattcct tatcgttaat ttataaataa ggggctaagg cctttgcaat
    22561 taccgcatag cccttacctg aaatatgcaa cccttcaact gtataacggc ggtcaatccg
    22621 ccctttttta tccgctaaaa gagtgtagaa atctaaatat ttaagtcctt cttcagcaca
    22681 aaaattaatt aagagttcat ttgtcttttt aattttctta ttcgttctta atccaacaat
    22741 aaatgtggat aaaaactttt tagtgcgtga tacgggataa agtgaaatgc aaattacttc
    22801 aatatttggt acttcttttt tgaatgttgt aataatcttt tttatattag cgactatttt
    22861 acgaggacta cttcctttgg ctaagtcatt cgtgccaatt tgtaaaaata acttgcgcgg
    22921 cgctagtgct actacattat caagcctttt taatacattt ttggtcgtat caccagcaat
    22981 accgcggtta tatatcttta cacccggaaa gaaatcttga atcggaaaaa agtctgttaa
    23041 actatcacct aaaaaagcaa tctttccgct tttggcttct ttgttaactt cttcaaatat
    23101 ttctaagcga ttaactgcat ttgtgtgtac gacaaataaa taaatggcaa caattaaaat
    23161 aagtgcgcct aagacactta ctaacacaat ttctagtgga ctcataataa gtagctcctc
    23221 ttttcaatta tctttatttt agcaaatata atataagact ttttataaat taaagacttt
    23281 tttacttttt gattataatc tcttccactt caaacattat aaaactattt ttgtactttg
    23341 tttatgcctt aagagcacaa atggttttca cataaaatta tgcgttacac gattgtttta
    23401 aaaagtaatt actatatttt gtggtgtttt gttgtttact tttgtggtat tttgccagtt
    23461 atatttgtgg taaaaaatgc agctaataaa taaagtatcg taactgtaca agtatcttat
    23521 tacttataat caacaattaa catatcatct gcaaagtaaa cattaaatat acatgcgcga
    23581 ctatcaggtg aaggtttatc aataggagta tgaactgaat caaaaaatag atttcgcttt
    23641 attgtttttt atatctttaa ctattaattt gtcacttttt tgtgagaaag gcacacttaa
    23701 aacaaaccta aattaactgt aaaattaaaa attttgcggg aaaatttaag ttttttagta
    23761 ataaaactat tactttaatg atattttaac caaaagcatc agcgaaaaac taccactttt
    23821 acaacttctt tacaactttt tacagcttta tgatatatct agctctccgt ttgctttaaa
    23881 gtttatgtgt ctgaaaggag gtgttcagat atgaaaaatg caatttgcag aatcggagaa
    23941 cttatggcga ttgctatggg tagtctcgag cttgtatcca tgcggattaa ggaagagcca
    24001 tttgatacaa gtaacactat tacccgttat acacgggaaa cttcttccac catacgtgac
    24061 agttttttta ccacgattga ttttaaagcc gctattagtg ataacactat tattaaagga
    24121 gctacactaa ctgcggatga tagcgatatg atctctttac tcaataacga tttatacggt
    24181 attggatata tttcctacgc cgcacttgaa tcttccgagc ttaaagcttt aataattgaa
    24241 ggggttcgcg tctccgaaaa atcaattatt gatgattcgt acaaattatc acgaaacttt
    24301 aactacatca caaaagctcc tagtgactta agtgaaaaag aatatgccct aattacaggg
    24361 ttcatcgcct ttagtaagtc acgcgaaggc ttaagtatta ttaaggccaa agacggaatc
    24421 gttacccaaa atattgaaga ggctgaaagt tttacaacac tcttagcgaa aagcgaaaat
    24481 agcgctctag cgaaaatgct ttataacact aatgaagcac caatccacat taaacttggt
    24541 ggttcaaaat ctgtcgaaag tattagtcgt gccttaagta gtgcttttac ttctttagtg
    24601 cccgccttta aagcagttca taactacact agtaatgaaa tggcttatga agcaacacaa
    24661 tggagtaata aagatagcgc tgaaccactt catattggtt ttttagcccg ggatttagat
    24721 attaatgaaa ctgaacccgc acttgctggt agctacggcg taattagtaa ggacgctatc
    24781 gttgctgtcg tcagcaataa aaaccatgtc cttagtgaca tcacacgcga tcaattaaaa
    24841 caaatttaca aatctgaaac aattacgtgg tccgcacttg aatagcgcat cacttgttat
    24901 ctaactgtca cgataacatt cttaaaggag cggtacatct tcataaaatg atgataaccg
    24961 ctctttttta tatttttaaa tttatacaat aactattaaa cttattagta acgacgctta
    25021 cgatttagga agaaaaaacc cgccacagcg ataaaggcca ttatgcctag tacatagaaa
    25081 ttttgtttat tatcaccacg aactgcgggt ataccgcttg gtggtagtgt acggtcaatg
    25141 aagtttgtaa atgtaaccgt tccatatttt tgtaaaatat atacataacg cgccatagct
    25201 tgtgacacta agtcaccact taatgttgca gtcgcatttt ttaagtccgc ttgcacatct
    25261 ggcgctaatt cattaaataa atttccgagt tcctcccgtt tatgacttga gatattatta
    25321 accgccccag ttgcgtcaca gtttcttgtt tcatagagaa aattgatggc gtaacttgtt
    25381 gcagcgtcat gaactggagt cattacactc tacgctgcta catcgtgttc atagagattc
    25441 ttatcagcgg gtaacacaag ttccggggtt gaaacatgct ctggttcgaa aggttgacat
    25501 ccattattaa aagtaacttt ttctggtcta tcttgtcctg aaatattaag tgcataatag
    25561 ccgtaagata attcatgtcc catatctact actttttgca tcatccggcc tggccaacct
    25621 cctaatggtt cagttccgtc tgtattctta ccataagcaa atacaacata ctcgttaaat
    25681 ccgccgttat ccactttaat atctaaatta cgatcgtaaa accttaaatt aacactcggt
    25741 gaatccacaa atttagcgta caatgtaatg tcacttgtta aatcttttgg aacatatgcc
    25801 gtttctaata aagcgtcttc ataccaacca acgaattttt tctctggttg ataaaatggt
    25861 gccggggtat aatttaaacc agtaaaggct gctgcttctt caattaaaag ttcgccataa
    25921 tattttgtta ctttatgata cgttgtacca gcattgtgaa agtaaatttt ctgcatttta
    25981 agatcataaa cgaccatata atttccagca gtatttacat gaaaactctc atgataacct
    26041 gaagaaggat caccaggcat aattacatca cttgcattcg acttatgatc atcaaaatca
    26101 ataaatccgc gttgataagg ttcatttaat gtttgtttgt acacaaagat tttaaaatca
    26161 tcagcgttaa aggctctagt aagtgaaaga taacggccat cataaccact gataccaact
    26221 aaacaattat taatatcgtg cgttgtccag tcaccaacag cagggccccg caatgcatac
    26281 gaagatgtac ctgttggtgt tgatggtgtt gccccgagta aacttgtata ctcgactttc
    26341 atccgccttg cgcccgcagg actaccccca gcgttatcag gattaaaaac ttgaacctta
    26401 atttctccgg gataaattgt aataaagtta gcatcgggcg aatcttcata tcgataaata
    26461 taatcgcgtg aaagcgatgg tgtggtatca aattgaaggt cttccattgg tgccccagta
    26521 ttatagtcat atattttgta atatccagcg tgcgactgcg taaaggtata tacataaata
    26581 tcagaaccgc cgggatattc cgtgaatttt tatgacactg atggttccca tccaattgaa
    26641 tcgccgacta agaccggtat cgtcgttgcc gcttttgttt ttaccgcgtc cttacgaaaa
    26701 aacgctggtg tgataattgc actaattaat agcgaaataa cggcgctcct agctaaccat
    26761 tttcgatttt tgttcatctt tttgattccc cctttttatc aaactaatgc gaacacggtg
    26821 attaatatta ctaaaataat aaatattttc aatttcactt ttctatttta attcaccatt
    26881 aacaagatag caaatatctt tatgcccata ggaacagcat aataaatgcc aaaattttgc
    26941 caaaaaaaga tataaaacaa ccctatttac atttctatta acaaaaaaag gtctgatatt
    27001 tcatcaaacc tcttgttata
//
LOCUS       DAOSTA010000013        24478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_2230_length_24478_cov_11.858412, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000013 DAOSTA010000000
VERSION     DAOSTA010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 24478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24478
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2230_length_24478_cov_11.858412"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(23..127)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(167..823)
                     /locus_tag="PLU08_02245"
     CDS             complement(167..823)
                     /locus_tag="PLU08_02245"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF003589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOQ70557.1"
                     /translation="MNEIKTWEDFWEREKDSLYLQNLKVFLNAQDPLTTYPPRDKIFN
                     AFKLTPLEDVKVVIIGQDPYHQPGQAMGLAFSVPSNVPLPPSLRNIFIEMADDVGGPV
                     RTDGDLTYLAKQGVFLINTILTVERSKPLSHNIPEYKTLIVKVLEELNKRPQPIVFIL
                     WGSYARSFKKYINGPNKLVIESNHPSPLSANRGGFFGTRPFSRANNFLAKHGSNVINW
                     "
     gene            889..1977
                     /locus_tag="PLU08_02250"
     CDS             889..1977
                     /locus_tag="PLU08_02250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOQ70558.1"
                     /translation="MKILIVFNHPAPYKVALFNGLSAKHDVHVIFERFKASNRNQSFY
                     NESNYKFTLHKIKGVSLGDENHLSFGVVRHLKKHKYDLIIMNGYSTLTEMLALRYLKR
                     KKIPYAFYINGGFANEKECKLKKKIKTYFISGAEAYFSPAKKANEYLTYYGAPADRIY
                     NYPYSTVYEKDVLEVKTPTATKVLYWKEKGIEATNFTICITSFIKRKNNEALIKAWKE
                     IPKDYALILVGEGYEEPMYREIIANEKLTNVLLLPFAPKHLVFEYLSHSDNAVYISNY
                     DIYGHVINEALAHGLNVLTNTKMVAAIDLIRDGQNGLIAYPEDDLVSRILLLLATDFF
                     NEAIATAKENTIEESVRVHLDILEKICE"
     gene            1968..3068
                     /locus_tag="PLU08_02255"
     CDS             1968..3068
                     /locus_tag="PLU08_02255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ70559.1"
                     /translation="MRIMYLTTARHPHDYEMFLKKYKKAPNPSNQNFHNKLINILRSK
                     FPLGAFSTRPMEKNIFLTQSKSDYFYYPGYINTPVLKRLGIITAGLQYYEARTPKVIF
                     VDTLNVTLLELARRLKKRHNVKVIGIVTDNPINITGASKNYSQDVFKKSLVCDGFISL
                     TQGLLRLFNPKQKPAIIVPGFIEEAESFTNKKEKYAFFAGALYPKYGVDALIKTFRKL
                     NPPFKLVIAGHGPLADDLLENKHPNIEFLGQVCPPVAFRLAQRAYVNINPRPIDEQVD
                     LYSVPSKVIDYINSGSVTISTENTEIRNLVGDNIYFIKDAEPHTILEALNAINKNYNY
                     YKQRAAEAKKALVKALDNKVLINKIEDLINKI"
     gene            3111..4604
                     /locus_tag="PLU08_02260"
     CDS             3111..4604
                     /locus_tag="PLU08_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOQ70560.1"
                     /translation="MQRKEMRAPVVFTLIRNVTIALLSFISFPFASQALGTAAMGVYN
                     WANTFVYYFLIISRLGIPLIAIRECAKVKDDKDKLSEKVQSFFVLQLITTALSFVLLS
                     TIMFIGKDTPMFKGDTWELIFLLSLNFLVGVFSFEWVYIALDRVFYMSVRSLATTILA
                     TLLIIVFVQNDHDLFIYAFINLIATFVTVTLNLLLLKREGVKLSFRRKVNLKHLTKSL
                     LFVFGITFLITAYNQNDSLLLGYLDDSFRTSGAYSVGIRGIEIVITIITSLSGIFVIR
                     ATSALKNDDMPAFHRVISYSTNITFFIGVPAVLFMIGLSPEIVGFIVKDSPYWDEAAI
                     NNAILAVVLLSSLMLTYSIHDGIYQQVLVPLKKEKIYFFTMLFVLILNIVASVLLALF
                     VFKDNPLFAVALVTLLSEFVVLIILFITAKEYVIRHIFSLNNLKIILSAGVSLGLVML
                     IKHFLPAMAYLATVGLCIFIGGIIYIVMLLLLKEDLVYAQVKGVKNG"
     gene            4597..5517
                     /locus_tag="PLU08_02265"
     CDS             4597..5517
                     /locus_tag="PLU08_02265"
                     /inference="COORDINATES: protein motif:HMM:NF025663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HOQ70561.1"
                     /translation="MANPNFKENKLKRLIAKSIQPLLRIFSKKTYVSWQYRYITGHKL
                     DWKNLKRYTEKLQYLRLYYYPRNASVVKAASRDGARERVKSKGLEKLLIPIVGIYDKV
                     EDIPFEALPKRFVVKATHACALNHICLDKDTLDVNALKNKMRRWLKMDYGKKTVEPHY
                     SKIKPRLIVEHYVGKSDSLPTEYKMHVFNGKARYLYVVSGRGKDIRYDNLYVDFTPFP
                     ESQFNHWSSSDEVPRKPKNYEELVKHAEMLASDLLFCRVDFFIVDDHIYFNEFTFTPA
                     KGTLIFDDDKADFIISEWFDISSAKKNKAA"
     gene            complement(5489..6469)
                     /locus_tag="PLU08_02270"
     CDS             complement(5489..6469)
                     /locus_tag="PLU08_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70562.1"
                     /translation="MRKNKWIALLISVLTLTGCKAVTETFEKGAFHTQDFSENYYTNI
                     PERYLTSNYEETVYEISDRININQPLDNAFDNAIRNDLVNQNLSLEEAIKLYGSAETK
                     AIKKEDFRNEIEYMEAIFNSLSSAQNDKASWINYAANNNLVKGKYSKAIQESFKRGIF
                     SKLTDGIIKCDGTGPKVRMQISEQGMGQTFEHELISYNRFVLSARGGTSVDYGSYGKA
                     RVTKAKVKINISFFIEKSTTNSASKHTVTFIVPEMHSDNEAILFTSIISFDLREVLGA
                     ETLKRVNGLMISYELLEHEFLKPGGVLDTSLNEEFALMLYEVMLPYSSWR"
     gene            complement(6456..7844)
                     /locus_tag="PLU08_02275"
     CDS             complement(6456..7844)
                     /locus_tag="PLU08_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70563.1"
                     /translation="MKDKIRESKLALISLFLALEVVLFIALDFGNIGPVFKALAFLLT
                     AILAPFFFEEMKVDLGQGLYVLLMPILFYALVTLVAPAYGSYPEMYSNEVFMQLPFIT
                     RLINLLGIVGLLLLGYFIGKSEVFATKHIFIVILAGLAAPVLISLFATLINYGFFHPL
                     VYKSLVTYYAGHEYEVSSQASLLYGFRIMVGDLKALASMAVIITSAGFGLLFVNEKKD
                     KFTIISLVVISAVGVLTLIFTASFAALIYLLPALIFALLLKFDLLKKLKNKVTLGVVI
                     ALVGLGAIIFLLTAYNIFNLQAVWQTNRVTRKLFFNGYMQRFYIIFKEAFGFRNLYGH
                     FAMTVEGYKVFPSGNFLFDAMWIDGLIGFVALLAFFVMFVIRLVKYFNKDKDERVLKV
                     VLISVLLTAFFRFMLRYPFKIFTYQEFSDINNFPLINSPLFLVTVFIAGYVFVLKEDK
                     KIEEEEVTHAEK"
     gene            complement(7844..8785)
                     /locus_tag="PLU08_02280"
     CDS             complement(7844..8785)
                     /locus_tag="PLU08_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70564.1"
                     /translation="MKRTRRSYIWEFLKSAIYVFRYKILRIKPPTNRIGKYKEITMQK
                     SNNAIEEAIRKGEPFAAIRFGAVEISALNNYEKIRLGFRRKYKKSVVYSIKNNAGVFP
                     PTQEIIDFYAKYLETNLKDTDYLGVSGVHMENYFFKHLTPDATPVQNWALDPLLGKWS
                     HLLAGKRVLVISPFAEQIEEQYEKRHLLFQAEPEILPEFTLVTIPAILTSGKAASKEF
                     DNWFEVLDYLKLEILKKDFDIALVGAGAYGTPLCLYIKSLGKMAIQSGGATQLLFGLM
                     GKRWEKREYVARHVNPHWSRPTVKPYGHKNIEGGCYW"
     gene            complement(8800..9486)
                     /locus_tag="PLU08_02285"
     CDS             complement(8800..9486)
                     /locus_tag="PLU08_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOQ70565.1"
                     /translation="MENEKYKRLLQIISEEDLIKIQNKRIIIFGLGGVGGNALEALVR
                     SGFRRFTIVDNDVVDITNLNRQLIATTKTIGMPKVKAAKKRMLEIEPNCNINAVFEAV
                     TPQNVANFNLEFYDYIVDAIDSFEDKMALIIYALKHNLPIISAMGAGNKIDPTKVIIT
                     KIEKTEGCPLAKKVRYRLRQEKLKGLMVVTSKELPLTNAGNKPGSSAFVPPAFGLAMA
                     SYILRNVISK"
     gene            complement(9446..10423)
                     /locus_tag="PLU08_02290"
     CDS             complement(9446..10423)
                     /locus_tag="PLU08_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ70566.1"
                     /translation="MKWVFLGLAILIFLVISLYFWTVMITFRMLFRRPKLPKKLVFPK
                     AFFGGVFDKHIPEIEANHKKNAKKACEIVTISSSAGKLKGRFYENENSNKIVIFIHGY
                     YSSGMRDIGYFGSLHKELNVSLLVIDQRANGESDGVYTSFGALERYDIREWIFYLNAR
                     YKGEKDIYLHGISMGAATSLLVTALAALPPSFKGVIADCGFSRTNGVIFFAGKRMLKI
                     KPKLTFWGLNLIAKLAGGFDLNKVNVSRELHKNMKIPILFIHGTADLFVPYNMSVKNY
                     KATSGPKKLVAFPDAGHCESYLSNKALYTEEMRKFIYGKRKVQKTTTNN"
     gene            complement(10491..11699)
                     /locus_tag="PLU08_02295"
     CDS             complement(10491..11699)
                     /locus_tag="PLU08_02295"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOQ70567.1"
                     /translation="MKKSLVYFQSGGPTAIINTSMYGVIKEAKRQTEYIDGIYGSLHG
                     IEGLLNDNLIDLRQEDEAQIELLKQTPAAALGTTRYKLSGDINHEDYQKILNNVKKHN
                     IGYMLVNGGNDSMDTCYKLSTFFRKMDFDCKVIGIPKTIDNDLAFTDHCLGYPSAARY
                     VVQSIQDIALDNKVYPKSKFVIVEVMGRHAGWLTAAASVIQDPELKPDYIYLPEEKFD
                     LEEFLARAKEVYERKKRALFVVSEGIETQMPNLDKSVDAFGHAQLGGFAAKLAGIVEE
                     RLCYSTRPIEFSLLQRAGTLAITETDQKEAIKVSTVAVKRIVAGKTGQMVIIDRKPGP
                     KYKVSYKLKNLRGIANVEHKMTPELIHDVNNGGKLFGEYLFPLIDKPIKLQLENGVAK
                     YTKLKKVKVK"
     gene            complement(11744..12952)
                     /gene="glyA"
                     /locus_tag="PLU08_02300"
     CDS             complement(11744..12952)
                     /gene="glyA"
                     /locus_tag="PLU08_02300"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006585103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOQ70568.1"
                     /translation="MEKLFELVALETKRQNEGLELIASENFTSKKVRALAGSVLTNKY
                     AEGYPGRRYYGGCHYIDEIEGTAIELAKKLFNAKYANVQPHSGSSANMIAYASVLELG
                     DSILTLSLDEGGHLTHGSPVNFSSKHYKVTHYGLKDDGTIDYEALREIALKLKPKLIL
                     AGFSAYPFIIDFARIGKIAKEVGAYFMVDMAHIAGLIAGGYHPSPFPYADIVTSTTHK
                     TLRGPRGGIILTNSEEIIKKVNFTCFPGMQGGPLENIIGAKAQSFYEALQPEFKEYIK
                     RVVENTKVASETLRALGAEASETETHLFLLNTKKSYGITGKDAEALLETIKITTNKNM
                     LPKDDERPNTTSGLRLGFAAVTTRGLTKVNAAETARIIHEFLSKKITKEEAMIRVNKI
                     VDTLQAIDSL"
     gene            13049..13786
                     /locus_tag="PLU08_02305"
     CDS             13049..13786
                     /locus_tag="PLU08_02305"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOQ70569.1"
                     /translation="MRIKEHPIFKVPLAHRGLHNETIDENSMPAFEAAVKAGFGIELD
                     VHITADNKIVVIHDNTTTRTTGVEKVVNDTTYKDLKKLRLLHTKTEIPLLKDVLKMVK
                     GKVPVLVEVKAENKVPPALVTEVLKVIEKYPYKDKLAMQSFNPYVARDLKKGSTGIPV
                     GQLMSDVLYGQSKLVHFMYRSLLVLKISKPDFFNYEVKYIHKRNIQRKRKRLPLITWT
                     IDDYDKEKTAIALADNYIFEHIEIKER"
     gene            13788..15272
                     /locus_tag="PLU08_02310"
     CDS             13788..15272
                     /locus_tag="PLU08_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ70570.1"
                     /translation="MELKLNMKRTFLVGLAFMSIMLFWTMYDTLVAKMLVDNFGLNQT
                     FSGFIMALDNILALFLLPLFGRISDKTRTKLGKRTPFIIIGTLAASILFVSIAFFDLY
                     QQIAVTKHGFSLIESIGTAENPLYQFTLGTEVYTNNVRSALATERAQLIFQQVTKQQP
                     GLLIGFIVVLFFVLVAMGIYRSPAVSLMPDVTPKPLRSKANAVINFMGTMGGAFAYVL
                     VFLLASVPIFDKTGYVATFATTSLLMLLLIAIFVATVKENAWYKEMVEITEKYHLDSL
                     DIDDEGLDVSQHIDDEKMPKDVRRSFILILSAVVLWFMGYNAATTKFSVYAFNHLGLE
                     QFSLPPLIGIITAALAFIPIGYISQKIGRRRMILFGIVVLTIALGSAVFITGETKVLM
                     YIIMGLVGISWASINVNSYPMVVEMSRHGNIGVYTGYYYSASMSAMIITPILSGALMD
                     ITGWYPVLFIYSAVFMGLAFIPMYFVKHGEPAPVVLKEKAQKDE"
     gene            15438..15722
                     /locus_tag="PLU08_02315"
     CDS             15438..15722
                     /locus_tag="PLU08_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70571.1"
                     /translation="MKKKKTMVRLWGDHIVISSLFIGLAISLAFVALTFVTIEVLAKH
                     VAIIAEKKKDFIYGFGTLAILVAFVINNFWIKPQRNIVKSSDLNKKEREG"
     gene            15726..17117
                     /locus_tag="PLU08_02320"
     CDS             15726..17117
                     /locus_tag="PLU08_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOQ70572.1"
                     /translation="MVITFSLILQMVGIALLMTALYLIIGIIPGTDETSVLVPMTLIL
                     VTLKLNPLVIMAGFIAAVVTLNIADSIPTAITSIPGGVMSTPLVGHSQTLKKHGLTSY
                     SVQWMMLGSLIGVLVSFPIALAFWGIFEFISVQTGLEISNFIKRYNEYIFLGGAILMS
                     LTSKEKVVSLLSIVPFGFATAFLRNPGLIINNTLLNTLIGGRTLSMTPFFLSITTGPL
                     VFALFELFFPSVFKNQKVEGKMTVILDDDVKKTKHGHKKIIAKTGIASALGSLLFFMS
                     PVGVTMMLGEFATKDEKDEVQQALQGVSAVNGLSSATYLAGIMISLFVFAFPISPAAL
                     GPGSVIFSPGGPIILEGNAGLAVPKGYLLIAIVIGLVIGLSLTVWLGMKYSHKMTELV
                     FKYISQETVLIFLFSIIALLSFAEAGLYGLIIVVSVGLLSGWLNKKGAGYGVQFMALY
                     AGGLFITILKALF"
     gene            17131..18021
                     /gene="mvk"
                     /locus_tag="PLU08_02325"
     CDS             17131..18021
                     /gene="mvk"
                     /locus_tag="PLU08_02325"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HOQ70573.1"
                     /translation="MDGYGLAKIILIGEHSVVYGHPAIAIPFYAMRSEVVLTPHEEFI
                     LDTKYYTGPLNNAVDELHGLVALITHMAEIYKKIKPVIISVRSSLPEKSGLGSSASVA
                     KAVIDAFNNHFHLELTHIDYFELLKISENIYHHNASGIDASTVIYEKPIIYQRPHIKP
                     LKFNIDGYLAVFYSNTNSATKSAVAHVSTHPERDIHIDALGSLTANAKWALENNDITL
                     LATMFNAAHTHLRALGISTPTLEELRTKLLDAGALGVKITGGGMGGCLIALFNDEEKA
                     LATKEKLKSFPSWMIDLRNM"
     gene            18021..18974
                     /gene="mvaD"
                     /locus_tag="PLU08_02330"
     CDS             18021..18974
                     /gene="mvaD"
                     /locus_tag="PLU08_02330"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HOQ70574.1"
                     /translation="MKYKKANVNIAILKYWGKVNALINIPYQTSISVTADNFYTLTNV
                     ELDPKATKDIVILDGTALSGISYTRVAHHLDALRKHFKRKEYCIVNSINRVFTKSGYA
                     SSASGFAALTAAYIDAIGAKVSKKEMSKLARLGSGSATRSIHGGFVIWHRGNSHDTSY
                     AEKLQVKWPEFRMLFTIIDPSVKKISSRVGMQITIEKAKSYADYVKQSNESVEPLITA
                     LKENDIDAVGRIAERAAELMRNVMLEAGLEYHTPKTQQLIQKVKAIRSKHKIPVYYTF
                     DAGPNLILFTLDEYVNEVIRLLPEVKISVSGVGGGITSARS"
     gene            18961..19938
                     /locus_tag="PLU08_02335"
     CDS             18961..19938
                     /locus_tag="PLU08_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70575.1"
                     /translation="MRVHEKVPGKLYVIGEYNVLKSGHGAIVAPVNLFLQGTFVANHK
                     TTIIEDGVAYPYQTLLENGCPHQLKNTVTTITFFNEYLALKNIPLQQFEYTIENNLIS
                     DEQVKFGLGSSAASVILTLKLLNRLYRTNLSPLVLFKMAVLIQRELNALTSGGDLACT
                     IFDMPLYYVRYDLNWLLNEKRTFELLEVAWPLLEIKLLTKLPTFLVGWTKETITPDTN
                     GTIDSAFFKKANKLVHHYLVTYDDIYIKQYQNLLDKLGLTRQGMITPRLEALVKTARS
                     LGVAAKISGAGYGDCGIAQINDQSVVEPLTNLWENEGIIVLDIWRKDNE"
     gene            19931..20908
                     /gene="fni"
                     /locus_tag="PLU08_02340"
     CDS             19931..20908
                     /gene="fni"
                     /locus_tag="PLU08_02340"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOQ70576.1"
                     /translation="MSRRRKDEHIAIAKMAKIRKNDFSHIRFIHHSIPSLSLKDIDLS
                     TSFLGHKFNYPFYINAMTGGAKKGDKINAKLAILAQHFGLPLFVGSQSLAFKNKESRL
                     AFETLRFNFPDLFIVANINPNFTAEMASDAVEILGANALAIHVNSVQELVMGEGDRDF
                     TKWKDNIANILKTVKVPVIVKEVGYGMHEPTLLLLQELGVKHIDISGMGGINFSLIEL
                     KRQKKKDSPLLDFGISTVDALLLARNYPSFEVLASGGINNASSIIKSLAMGAKAAGMA
                     KYFLDLCNLPVPEMIKKVDELIIDLKKILVLLNVKSVHELNLDHLMFDL"
     gene            complement(20903..22264)
                     /locus_tag="PLU08_02345"
     CDS             complement(20903..22264)
                     /locus_tag="PLU08_02345"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ70577.1"
                     /translation="MKVKLVAQLRFLLSLFELLFGELQEIPGSTLGDILVLLNTIVLS
                     FLSVLYFYQVLLSIFSVVVRAKKYPETDVFPTYTFVTCARNEETVIAQLITTIRALDY
                     PQEKINIHVVADNCTDNTAIIAAQLKAKVYTRNDLTKIGKSYALEYYFNEQKKVVPAE
                     DFAGYIVIDTDNVLDAQFLKEVNKVYQATNAKVVATYRASTNAGDSLWAFGTGYSFLR
                     ECCLMHKGREVLGLSSYVSGTGFFVSYEKMQEVEGWNHHLLIEDIEFSAAHVMEKGRT
                     HYAHNAIFYDEQAVNFKSSWHQRMRWVKGLFQVSKVYTWSLFKSIFARKRTLKERFSS
                     YEAFLFSTPFPAYIIWWFILYGIGAAINLAITQDINYFIQTYLFTLFDFLLGFYLFTT
                     LMSLLISVSNWKRIKMHPLKKIMLPFVAFFFLLSYVPMLLIAPFVKVTWRPVKHYGLK
                     KPL"
     gene            22368..22877
                     /locus_tag="PLU08_02350"
     CDS             22368..22877
                     /locus_tag="PLU08_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ70578.1"
                     /translation="MLKTNLLRAFSRRCFLLYISVFFLTSLGFGTDKLGLIAALSPFA
                     ALLGNIIFQRIAKNLEVNRILMFIFSIIETSIALIFFIFPKQPFIFYAIIITCVSTIN
                     GPFYSLLDGYSGTYISERNKQYSSMRVMGTLSYVIGPLIGGFLVDNMVLDFKHYFSCH
                     QCFTSLLPF"
     gene            22841..23572
                     /locus_tag="PLU08_02355"
     CDS             22841..23572
                     /locus_tag="PLU08_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70579.1"
                     /translation="MSSVFYIIASFLIYYLPKQKIELHIIVPPEEKKRLKNIGKPELI
                     AYLLFNFFVIGVTVVTDNFFGIYLRTELGVTTTQFGYIVAIAIAIEAGVMFLLALRKN
                     NSFRVPAFSFFFIGTFLLIRPLVIVLNLSPTLTMILSVFRGIAWGYYIVFNVRFLARV
                     IPLKHLTKALLTGSIATTSSRIITSLIVGESLKHHSYQTVFFFSMFSVVFGMTISIII
                     AEIDRRKQKKVDMIVPFIGSDKDLN"
     gene            complement(23569..24054)
                     /locus_tag="PLU08_02360"
     CDS             complement(23569..24054)
                     /locus_tag="PLU08_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HOQ70580.1"
                     /translation="MEIRDVTNVLFIKNNKVLLGYKKRGFGKKKYNGFGGKLKSGETI
                     EMAAIREAKEEAGLTMKTYYKAAEIDFAESYPLRMHLYVCTEWSGEITESDEMVPRWF
                     GFNEVPVDEMWDDDKYWLHLALSGQKFRASFVFENDDDVTGTAENKVISYSIKLVDTL
                     D"
     gene            complement(24191..24394)
                     /locus_tag="PLU08_02365"
     CDS             complement(24191..24394)
                     /locus_tag="PLU08_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70581.1"
                     /translation="MVKEFGLPWIAHLLLQFFIGPIWGAVIRLVRGRDLWAVIYLLTG
                     GFFVIGWIYDLVMLSIHRDYKLA"
BASE COUNT         7661 a         4354 c         4246 g         8217 t
ORIGIN      
        1 aaaaagtctc cacgctgacg tgaagactct tatgttacat ccctacgcca gcattatctg
       61 tatcaggtgc ggtcgaacgt tcattgttcc tctcagccag gcacacctag ctcccgtgag
      121 ttcattatat caaatcgcaa actttgtagt gtttttttgt cttatcttac caattaatta
      181 catttgaccc gtgttttgca aggaaattat tagcacgcga gaaaggtcgt gttccaaaaa
      241 agccacctct attcgcggat aatggcgaag ggtgatttga ctcaatcact aatttattag
      301 gaccatttat atatttctta aatgaccttg cataactccc ccataagata aagacaatcg
      361 gttggggtct cttatttaat tcttcaagta ctttaacaat taatgtttta tattccggaa
      421 tgttatgtga aagtggttta cttctttcaa cagttaaaat ggtattaata agaaatacgc
      481 cttgtttagc taaataagtt aaatctccgt ccgtgcgaac tggtccaccc acatcatcag
      541 ccatttcaat aaatatattt cttaagcttg gcgggagcgg cacatttgaa ggcacactaa
      601 aagctagtcc cattgcttgg cctggttggt gataaggatc ttgcccaata atcacaactt
      661 tgacatcttc tagcggtgtt aatttgaaag cgttaaaaat tttatcgcgc ggtggatatg
      721 ttgttagggg gtcttgggca ttaagaaaaa cttttaaatt ttgtaaataa agtgagtctt
      781 tttcgcgttc ccaaaagtct tcccaagttt taatttcatt cataaaataa ttatatcatc
      841 attgctttgc aatgtttctt ggcttaagtt gttgtataat ttatacatat gaaaatatta
      901 attgtgttta atcatcctgc cccatataaa gtcgcactct ttaatggttt aagtgcaaaa
      961 catgatgtgc atgttatttt tgaacgcttt aaagcaagca atcgtaatca aagtttttac
     1021 aatgaatcaa attataaatt taccctccat aaaataaagg gtgtctctct tggcgacgaa
     1081 aatcatcttt cgtttggtgt agtgcgtcac ttaaagaagc ataaatacga cttaattatt
     1141 atgaatggtt actcaacatt aacagaaatg cttgcacttc gttatttaaa gcgtaaaaaa
     1201 attccttatg ctttttatat taatggcggt ttcgctaatg agaaagaatg taagcttaaa
     1261 aagaaaatta aaacttattt tattagcggc gcagaagcct actttagtcc tgctaaaaag
     1321 gcaaatgagt acttaactta ttacggagcg cccgctgata ggatttataa ttatccttac
     1381 tccacagttt atgaaaaaga tgttttagaa gttaaaacac caaccgctac taaagttttg
     1441 tattggaaag aaaaaggaat tgaagcaact aattttacga tttgtataac tagttttatt
     1501 aaacgtaaaa ataatgaagc gcttattaaa gcgtggaaag aaataccaaa agactatgcc
     1561 ctcattctag taggtgaagg ctatgaagaa ccgatgtatc gcgaaattat tgcaaatgag
     1621 aaattaacaa atgtacttct gcttccgttt gcgcctaaac accttgtatt tgaatattta
     1681 tcacatagtg ataatgctgt ttatattagt aattacgata tttatggtca tgtcattaat
     1741 gaagcactag cgcacgggct taatgtttta acaaatacaa aaatggtggc cgcaattgac
     1801 ttaattcgtg acggacaaaa cggtttaatt gcttatccag aagacgattt agtaagcaga
     1861 atattactac tgcttgcgac tgacttcttt aatgaagcaa tcgctaccgc aaaagaaaac
     1921 acaattgaag aatcagtgcg agtgcactta gatatattgg agaaaatatg cgaataatgt
     1981 atttaacaac tgctcgtcat ccgcatgatt acgagatgtt tctaaaaaaa tataaaaaag
     2041 ctcctaatcc aagtaatcaa aactttcata ataaattaat taatatttta cggagtaaat
     2101 ttccgcttgg tgcctttagt acacgcccga tggaaaagaa tattttctta acgcaaagta
     2161 aaagtgatta tttttattat cctggttata ttaatactcc cgttcttaaa cggcttggca
     2221 ttattactgc tggattacaa tactatgaag cacgaacacc aaaagttata tttgttgata
     2281 cattaaatgt gacgcttctt gaattagcgc gtagattaaa gaaaagacat aatgttaaag
     2341 ttattgggat cgttactgat aacccaataa atattactgg tgcaagtaaa aactatagcc
     2401 aagatgtatt taagaagagt ctcgtgtgtg atggcttcat tagtttaact caaggactct
     2461 tacgcctttt taatccgaaa caaaaaccag caattattgt tccgggcttt attgaagagg
     2521 ctgaaagttt caccaataaa aaggaaaaat acgccttttt tgctggagca ttatacccaa
     2581 aatacggagt tgatgcttta attaaaacat tccgtaaact taatccgcct tttaaattag
     2641 tcatagcggg acacggcccg cttgcggatg atttacttga aaataagcat cctaatattg
     2701 agttccttgg tcaagtttgt ccgcctgttg cctttcgttt agcgcaaagg gcgtatgtca
     2761 atattaatcc gcggccaata gatgaacagg ttgatctcta ttcagtacca agtaaagtta
     2821 ttgattacat caatagcgga agtgttacta tttccactga aaacacggaa attagaaact
     2881 tggtagggga taatatttac tttataaaag acgctgaacc acacacaatt cttgaagcac
     2941 ttaacgctat taacaaaaat tataactatt ataaacaaag ggccgctgaa gctaaaaaag
     3001 ctttagtaaa agcacttgat aacaaagttc ttattaataa aattgaagat ttaattaata
     3061 aaatctagta aatccgccat tattcattaa aaatatcata taatttactt atgcaacgaa
     3121 aagagatgcg cgcccctgtt gtttttacac taattcgtaa tgtaacaatt gcgctccttt
     3181 cttttatttc ttttcccttt gcctcacaag cacttggtac tgcggcgatg ggggtatata
     3241 actgggcaaa tacttttgta tattactttt taattatctc gcggctcggg attccgctta
     3301 ttgcgattag ggaatgcgca aaggtgaaag atgataaaga taaactaagc gaaaaagttc
     3361 agtctttctt tgtgctgcaa ttaattacga cagcactttc tttcgtgctt ttatccacaa
     3421 ttatgtttat tggtaaagat acaccgatgt ttaaaggtga tacttgggag ttaatctttt
     3481 tattatcact aaacttcctc gtgggcgtct tctcctttga atgggtatat attgctctag
     3541 atcgagtatt ttatatgtct gttcgttctt tagcgacaac aattttagct acattactga
     3601 ttattgtttt cgtacaaaat gaccacgatt tatttattta tgcttttatt aacttaatcg
     3661 ctacttttgt aactgtgact ttaaaccttc tcttattaaa acgcgaaggt gtaaaacttt
     3721 cctttagacg aaaggttaat ttaaaacact taacaaagtc gcttctcttt gtatttggta
     3781 ttactttctt aattaccgct tataaccaaa atgattcttt actccttggt tacttagatg
     3841 attcttttag aacatctggt gcttattctg ttggaattcg cggtattgaa attgttatta
     3901 ccattattac ttctttaagt ggcatctttg tgatccgcgc tacttctgca cttaaaaatg
     3961 atgatatgcc tgctttccat cgtgttataa gttacagcac taatattacc ttttttattg
     4021 gtgtacctgc ggtcttattt atgattggct tatcaccgga aattgtcggc tttattgtta
     4081 aagatagtcc ttattgggat gaagcagcga ttaataacgc cattttagcg gttgttctgc
     4141 tttcatcttt aatgctaact tacagtattc atgacggtat atatcagcaa gtgcttgttc
     4201 cgcttaaaaa agaaaaaata tacttcttta ctatgttatt tgtgcttatt ttaaatatcg
     4261 ttgcaagtgt attacttgcc ttattcgtgt ttaaagataa tccgcttttt gcggttgcgc
     4321 ttgttacact gctttccgaa tttgtagtat taattatttt atttattacc gcaaaagaat
     4381 atgttattcg tcatatattt agtcttaata atctgaagat tattttatct gcaggtgtta
     4441 gtcttggttt agtaatgtta ataaaacact tcttaccagc aatggcttac ttagcgactg
     4501 tggggctttg tatattcatc ggcggaatca tttatatcgt tatgttacta ttattaaaag
     4561 aagatttagt gtacgctcaa gttaaaggag taaaaaatgg ctaatcctaa ttttaaagaa
     4621 aataagttaa aacgcttgat tgctaaaagt attcaaccgc ttttacgtat attttccaag
     4681 aaaacatatg tgtcgtggca gtatcgttat attactggtc ataaattaga ctggaaaaat
     4741 ttaaagcgtt atactgaaaa acttcaatac ttacggctct actactatcc gcgtaacgca
     4801 agtgttgtta aagctgcttc gcgtgacggg gcacgggaaa gagtaaaaag taaggggctt
     4861 gaaaaacttt taattccaat tgtggggatt tacgataaag ttgaagatat cccgtttgag
     4921 gcattaccaa agcgctttgt cgttaaagcg acgcacgctt gtgctttaaa tcatatttgt
     4981 ttagataaag acacacttga tgtaaacgca ctaaagaaca aaatgcgtag atggttaaaa
     5041 atggattacg gtaaaaaaac agtggaacca cattactcaa aaataaagcc gcgcttaata
     5101 gtggagcatt atgttgggaa aagtgatagt ttaccaactg aatataaaat gcatgtcttt
     5161 aacggtaagg cccgctactt atatgtcgtt agtggacgcg gtaaagatat acgttatgat
     5221 aatttatatg tcgattttac accttttcct gaatcgcaat ttaatcactg gtcaagtagt
     5281 gatgaagtgc caaggaaacc taaaaactat gaagagcttg taaagcatgc ggaaatgctc
     5341 gctagtgatt tattattctg tcgcgttgat ttttttattg ttgatgatca tatttacttt
     5401 aatgaattta cttttacacc tgcaaaagga accttaattt ttgatgatga taaggctgac
     5461 tttatcatta gtgaatggtt tgatatttct agcgccaaga agaataaggc agcataactt
     5521 catagagcat taatgcgaac tcttcattaa gtgaagtatc aagtactcca cctggtttca
     5581 gaaattcatg ttctaataat tcgtaactta tcattagtcc gtttactctt ttaagtgttt
     5641 ccgcacctaa tacttcgcgg agatcaaaac taataatact cgtaaataaa attgcttcat
     5701 tatcactgtg catctcaggg acaataaatg taacggtgtg tttagaagca ctgtttgtcg
     5761 tgcttttttc aataaagaaa ctaatattaa tttttacttt agctttagtg acgcgcgctt
     5821 taccgtaact tccataatca acacttgtac caccgcgagc gcttaacaca aaacggttat
     5881 aactaataag ttcatgttca aaagtttgac ccatgccttg ttcactaatt tgcatcctaa
     5941 cttttggacc agtgccgtca cacttaataa taccgtctgt taacttcgaa aaaattccgc
     6001 gtttaaatga ttcttgaatt gctttactat attttccttt aacaagatta ttattagcgg
     6061 cgtaattaat ccagctcgcc ttatcatttt gtgcactgct caaactatta aagattgctt
     6121 ccatatattc aatttcatta cggaagtctt ctttttttat cgctttagtt tcagcactac
     6181 cgtataactt aattgcctct tcaagtgata aattttggtt aactaagtca tttctaattg
     6241 cattatcaaa agcattatca agtggttgat taatatttat acgatcacta atttcataaa
     6301 ctgtttcttc gtaattactt gttaaataac gttctgggat atttgtataa taattttcac
     6361 taaagtcttg agtgtgaaat gcaccttttt caaaggtttc cgtaactgct ttacaacctg
     6421 taagtgttaa gacactaata aggagtgcta tccatttatt tttccgcatg agttacttcc
     6481 tcctcttcta tttttttatc ttcttttaag acaaaaacat accccgcaat aaagactgtc
     6541 actaaaaata gtggtgaatt aatcagcggg aaattattaa tgtcactaaa ttcttgataa
     6601 gtaaagattt taaaaggata acggagcata aagcggaaga atgctgtgag taacacgcta
     6661 atgagtacaa cctttaatac tctctcatcc ttatctttat tgaaatactt cactaaacga
     6721 atcacaaaca tcacaaagaa tgcaagtaga gcaacaaacc caattaaacc gtcaatccac
     6781 attgcatcaa ataagaagtt acctgaaggg aatactttat aaccttcaac tgtcatcgca
     6841 aagtgtccat ataaattacg gaatccaaat gcttctttga aaataatata gaagcgttgc
     6901 atgtagccgt taaagaataa tttacgcgta acgcgattag tttgccacac tgcttgtaag
     6961 ttaaagatat tataagcagt tagtaagaaa ataatagcgc caagtcccac tagggcaata
     7021 acgacaccaa gcgtcacttt gtttttaagt ttctttaaga ggtcaaactt aagtaacaaa
     7081 gcaaaaataa gcgctggtaa taaataaata agagccgcaa acgaggcggt gaatattaag
     7141 gtaaggacac caacagcact aataactacg agactaatga ttgtaaattt atctttcttt
     7201 tcatttacaa acaataagcc aaagcctgcg ctagtaataa taaccgccat cgatgccagt
     7261 gcttttaagt caccaaccat tattctaaag ccatacaata atgacgcttg actactaact
     7321 tcatattcat gtcctgcgta ataagttact aaacttttgt acactaacgg gtgaaaaaat
     7381 ccgtaattaa taagtgtagc aaaaagcgaa attaaaactg gtgctgctag tccggcaaga
     7441 ataacaataa atatatgttt tgtggcaaat acctcacttt tgccgataaa atagcccaat
     7501 aataaaagtc caacaattcc taacaaattt attaatcgcg taataaatgg tagttgcata
     7561 aatacttcat ttgaatacat ttcaggataa gaaccgtatg ctggtgcgac tagcgtcact
     7621 aacgcataaa aaagaatcgg cattaatagc acatataaac cttggcctaa atcaactttc
     7681 atttcttcaa aaaagaaagg cgcaagaatc gctgttaaca aaaacgctaa tgctttaaat
     7741 accggaccaa tattaccaaa atcaagcgca ataaaaagca ctacttctaa tgctagaaat
     7801 aatgaaataa gcgccaattt actttctcta attttatctt tcattaccag taacaccctc
     7861 cttcaatgtt tttatgacca tatggtttaa cagttggccg tgaccaatgt ggattaacgt
     7921 gacgcgcaac atattcgcgc ttttcccatc ttttacccat aagtccaaaa agtaattgtg
     7981 ttgcaccgcc actttgaatt gccattttgc ctaagctttt aatatataga caaagcggag
     8041 tgccgtaagc ccccgccccg acaagagcaa tatcaaaatc cttctttaaa atctctagtt
     8101 ttaaataatc aagtacttca aaccaattat caaattcttt acttgctgct ttaccagaag
     8161 ttaaaatcgc tggaatcgtc actagtgtaa attccggtaa tatttctggt tctgcttgaa
     8221 aaagcaggtg tcttttttca tattgttctt cgatttgttc ggcaaatggt gatataacaa
     8281 gtacgcgttt gcctgctaaa agatgtgacc atttaccaag aagcggatct agtgcccaat
     8341 tttgcacggg agtagcatct ggtgttaagt gtttaaagaa ataattttcc atatggacac
     8401 cgctaactcc taaataatcc gtatctttta agttagtctc aagatattta gcgtaaaaat
     8461 caataatttc ttgcgtaggc ggaaataccc cagcgttatt tttaatgctg tatacaacgg
     8521 actttttata tttccgtctg aaaccgagac ggattttctc ataattatta agtgcactta
     8581 tttctacagc gccaaatcga atcgccgcga aaggttcgcc tttccggatt gcttcttcaa
     8641 tcgcgttgtt acttttttgc atcgtgattt ctttatattt accgatgcga tttgttggtg
     8701 gcttaattct taaaatttta tagcgaaaaa catatattgc gctttttaag aattcccata
     8761 tatatgagcg acgtgttctt ttcatacatt tatcatacac tatttagata ttacatttct
     8821 caaaatgtaa cttgccatcg ctaatccaaa ggctggtggg acaaaggcac tacttcctgg
     8881 tttattacca gcattagtta atggtaattc ttttgaagta acaaccatta atcctttcag
     8941 tttttcttga cgtaagcgat aacgcacttt tttcgctagt ggacaccctt ctgttttttc
     9001 tattttagta ataattactt tagttggatc aatcttgttg cccgcaccca ttgcactaat
     9061 aataggcaaa ttatgtttta aagcataaat aataagtgcc atcttatctt caaaactatc
     9121 aatagcgtca acaatataat cataaaattc taaattaaaa ttagcgacat tttgcggagt
     9181 aaccgcttca aaaacggcat taatattgca atttggctct atttctaaca tacgcttctt
     9241 tgccgctttt acttttggca taccaatcgt ttttgttgta gcaattaatt gtcgattaag
     9301 attagtaata tcaacgacat cattatcaac aattgtaaag cgtctaaaac cggagcggac
     9361 aagcgcttca agcgcattac caccaacgcc gccaagtcca aaaataatga tgcgtttatt
     9421 ttggatttta ataaggtctt cttcactaat tatttgtagt agtcttttgt acttttcgtt
     9481 ttccatatat aaactttctc atttcttcgg tgtagagtgc tttatttgat aaatatgatt
     9541 cacaatgacc tgcatcagga aacgccacta gttttttggg accacttgtt gccttataat
     9601 ttttaactga catattataa ggaacaaata aatcagcggt accatgaata aataaaattg
     9661 gtatcttcat gtttttatgt aattcgcgac ttacattaac tttattaaga tcaaagccgc
     9721 ccgctaattt agctattagg ttgagtcccc aaaaagtaag tttcggttta atcttaagca
     9781 tgcgcttacc ggcaaaaaag attacaccat tagtgcgcga aaatccgcaa tcagcgataa
     9841 cacctttaaa gctcggtggt aatgccgcta gtgctgtaac gagtaagctt gtcgcagccc
     9901 ccattgaaat tccgtgaaga taaatatctt tttcgccttt ataacgagca tttaaataaa
     9961 agatccattc cctaatatca taacgctcaa gcgccccaaa tgatgtataa acaccatcac
    10021 tctccccgtt agcgcgttgg tcaatgacaa gtagtgacac atttaactct ttatgtaatg
    10081 aaccaaaata acctatatca cgcattccag aagaataata accgtgaata aaaataacta
    10141 tcttattact attctcgttt tcatagaatc gtccttttaa tttacccgca cttgaactaa
    10201 tagttactat ttcacatgcc tttttagcat ttttcttatg gttagcctct atttccggaa
    10261 tatgtttatc aaaaacacca ccaaaaaaag cttttgggaa cactagtttc ttaggaagtt
    10321 ttggccgacg aaagagcatc cggaatgtaa tcatgacagt ccaaaaatac agtgatatta
    10381 cgagaaatat taagattgct agccctaaaa atacccattt catacattta ttataacttt
    10441 taataaagaa aaagcggagt ttatactccg ctcactattt tttttactaa ttatttaact
    10501 ttaacttttt taagtttagt gtatttagcg acaccatttt ctaattgaag tttgattggt
    10561 ttatcgatta gtgggaataa atattcacca aataacttac cgccattatt aacatcgtga
    10621 atgagttctg gtgtcatttt atgttcaaca ttagcaatac cgcgtaagtt tttaagttta
    10681 taacttactt tatattttgg tcctggttta cggtcgataa taaccatttg tcctgtttta
    10741 ccagcaacaa tacgtttgac cgcaacagtt gaaactttaa ttgcttcttt ttggtctgtt
    10801 tcggtaattg ctaaagtacc tgctctttgt aaaagactaa attcaattgg gcgcgttgaa
    10861 tagcataagc gttcttcaac gatacctgct aatttggcag caaagccacc aagttgagca
    10921 tgaccaaatg cgtcgactga tttatcaagg ttaggcattt gtgtttcaat accttcacta
    10981 acgacaaata atgcccgttt tttgcgttca taaacttctt tagcgcgcgc taagaattct
    11041 tctaagtcaa atttttcttc aggtaaataa atatagtctg gtttaagttc tggatcttgg
    11101 ataacacttg cggccgcagt taaccatcca gcgtggcgcc ccatcacttc gacgataacg
    11161 aattttgatt ttggatacac tttattatct aaagcgatgt cttgaattga ttggacaaca
    11221 taacgggctg cacttggata acctaagcag tgatcagtaa acgctaagtc gttgtcaatt
    11281 gtctttggaa taccgattac tttacaatcg aagtccattt tacggaagaa agttgataat
    11341 ttgtaacaag tatccattga atcgttacca ccattaacta acatgtaacc aatgttatgt
    11401 tttttaacgt tgtttaatat tttttggtaa tcttcatgat taatatcacc agaaagttta
    11461 tagcgcgttg tccctaacgc cgcagcaggg gtttgtttta ataattcaat ttgcgcttca
    11521 tcttcttgtc gtaaatcaat aagattgtcg tttaataaac cttcaatacc gtgtaaacta
    11581 ccataaattc catcgatata ttcggtttgc cgtttggctt ctttgatgac accatacatt
    11641 gaggtgttaa tgattgcggt tggtccgcct gattggaaat acactaatga ttttttcata
    11701 tttttactcc ttttctcacc cgagattata ccatgagtga ctattaaaga ctatcgatag
    11761 cctgtaaagt atcaacaatt ttattaactc taatcatcgc ttcttctttc gtaatcttct
    11821 tacttaagaa ttcatgaatg attcttgcgg tttcggcagc attaacctta gttaatccgc
    11881 gtgttgttac cgccgcaaag cctaggcgaa gaccagatgt cgtatttggt ctttcatcat
    11941 ccttaggtaa catattttta ttcgttgtaa ttttaattgt ttcaagtagt gcttcagcgt
    12001 cttttccagt aataccatac gactttttag tgttaagtaa aaagaggtgt gtttctgttt
    12061 cactcgcttc ggcaccaagg gcacgaagtg tttcactagc gactttagtg ttttctacaa
    12121 cacgcttaat atattcttta aattcgggtt gcagcgcttc ataaaatgat tgcgcctttg
    12181 cgccaataat attttcaagt ggtccgcctt gcataccagg gaaacaagta aaattaactt
    12241 ttttaataat ttcttcacta ttggtaagga taataccacc gcgcggtcct cttaatgttt
    12301 tatgggttgt acttgttact atatcagcgt aagggaaagg actagggtga tagccaccag
    12361 cgattaatcc tgcaatatga gccatatcaa ccataaaata cgccccaact tctttagcaa
    12421 ttttaccaat acgcgcaaaa tcaataataa aaggataagc agaaaatccc gcaagaataa
    12481 gttttggttt aagttttaaa gcaatttcgc gtaatgcttc gtaatcaatt gtgccgtcat
    12541 cctttaaacc gtaatgcgtc actttataat gttttgatga gaagttaaca gggctcccgt
    12601 gtgttaagtg cccgccttca tcaagtgaga gggttaagat gctatcacct aactctaata
    12661 cactagcgta agcaatcata tttgcactac taccactgtg tggttgtaca tttgcgtact
    12721 tagcgttaaa tagttttttt gctaattcaa tagcggtgcc ttcaatttca tcaatataat
    12781 gacatccgcc gtaataccta cgccctggat aaccttcagc gtacttattt gttaatactg
    12841 atcccgctaa tgcacgaact tttttacttg taaagttttc actagcgatt aattccaatc
    12901 cctcattttg tctttttgtt tctagcgcta ctaattcaaa taatttttcc ataacttaga
    12961 ccatccttac agtattagat tatagactaa tctagtctaa aaagtgctgt taattgactt
    13021 tttatggtat tttcgtgaaa ataaatacat gcgtattaaa gaacatccaa tattcaaagt
    13081 cccacttgca catcgcggac ttcataatga gacaattgac gaaaactcga tgcctgcttt
    13141 tgaggcggcg gtaaaagctg gttttggcat tgaacttgat gtgcatatta ccgctgataa
    13201 taagattgtt gtcattcacg acaatacgac tactagaaca acgggagtag aaaaagtagt
    13261 aaatgacact acttacaaag atttaaagaa attaagacta ctacatacaa agactgaaat
    13321 accattatta aaagatgttt taaagatggt taaaggtaaa gttcctgttt tagtggaagt
    13381 taaagccgaa aataaagtcc cacctgcact tgtcaccgaa gtgttaaaag ttatcgaaaa
    13441 gtatccgtat aaagataaac tagcgatgca gtctttcaac ccatatgttg cgcgtgactt
    13501 aaaaaagggg agcaccggta ttcctgttgg gcaactaatg agtgatgtac tgtacggtca
    13561 atcaaaatta gtgcacttta tgtatcgttc acttctagtc cttaaaatat ctaaaccaga
    13621 cttcttcaat tatgaagtaa aatatattca taagcgaaac attcagagaa aacgcaaacg
    13681 cttaccactt ataacatgga caattgatga ctacgataaa gaaaaaacag ctattgccct
    13741 agctgataac tatatttttg aacatattga aataaaggag agataatatg gaactaaaac
    13801 ttaacatgaa gcgcacattc ctcgtcggat tagcgtttat gtcaattatg ctgttctgga
    13861 cgatgtatga cacactcgtg gccaaaatgc ttgttgacaa ctttgggctc aatcaaacct
    13921 tctcagggtt tattatggca cttgataaca tcttagcgtt attcttgcta ccactatttg
    13981 ggcgaatcag cgacaaaacg cggaccaaat taggcaaaag aacaccgttt attattatcg
    14041 gtactttagc cgcttctatt ctttttgtta gcattgcctt ctttgattta tatcaacaaa
    14101 ttgccgttac taaacacggt ttctcattaa ttgaatcaat tggcactgct gaaaatccgt
    14161 tataccaatt cacacttgga actgaagtat atacaaacaa tgtacgttct gctcttgcaa
    14221 ccgagcgcgc acaattaatt ttccaacaag taacaaaaca acaacctggt ttacttattg
    14281 gctttattgt tgttttattc tttgtcttag tggcaatggg aatttaccgc agtcctgctg
    14341 ttagcttaat gccagacgtt acacctaaac cactccgtag taaagctaat gctgtcatta
    14401 actttatggg gacaatgggt ggtgcgtttg cctatgtcct agtattctta cttgctagcg
    14461 ttcctatctt tgataaaacg ggctatgtcg ctacttttgc aacaactagt ttactaatgc
    14521 ttttattaat tgctatcttt gttgcgactg ttaaagaaaa cgcatggtat aaagaaatgg
    14581 tcgaaatcac tgaaaagtat caccttgatt cattagatat tgatgacgaa ggtttagatg
    14641 tatcacaaca tattgatgac gaaaaaatgc caaaagatgt acgccgtagt tttattctca
    14701 tcctaagtgc tgttgtatta tggtttatgg gttataacgc tgcgactaca aagttttctg
    14761 tttatgcctt taaccattta ggacttgaac aatttagttt accaccactt attggcatta
    14821 ttaccgctgc gttagcgttt attccaattg gttacatttc acaaaaaatt ggtcgtcgcc
    14881 gtatgattct cttcggtatt gttgtgttaa caattgcttt aggtagtgcc gtctttatta
    14941 ctggtgaaac taaagtatta atgtacatta tcatgggtct tgtcggcatt agttgggctt
    15001 caattaatgt taatagttat ccgatggttg tggaaatgtc gcgtcacggt aatatcggtg
    15061 tttacactgg ttactactat agcgctagta tgagtgcaat gattattaca ccgattcttt
    15121 ctggggcatt aatggatatt accggttggt acccagtact ctttatttat agtgcggtct
    15181 ttatgggcct agcgttcatt ccaatgtact ttgttaagca cggtgaacct gcgccagtag
    15241 tgttaaaaga aaaagcacaa aaagacgaat aattcgctaa atacactatc gaaagatgac
    15301 cctagtgggg tcatcttttt tattattatc attattactt ttcaattatt tcttactgaa
    15361 taattgattt ttgcttatgt gttttgggat taattactta aaatgctata atgatctttc
    15421 aagcgaggtt tgatgttatg aaaaagaaga aaacgatggt gcgcttgtgg ggagatcaca
    15481 ttgtaatatc atcactgttt atagggcttg ctattagcct agccttcgtg gcattaacat
    15541 ttgttactat tgaagtatta gcaaaacatg ttgcgatcat tgccgaaaaa aagaaagatt
    15601 ttatttatgg gtttgggaca ttagcgattc ttgttgcttt tgtcattaat aatttttgga
    15661 ttaagcccca acgcaatatt gttaaatcaa gtgatttaaa caagaaagaa agagagggct
    15721 aatttatggt tattactttc tctttaattt tacaaatggt cgggattgct ctccttatga
    15781 ccgctttata tctgattatc ggtattatcc ctggcactga tgaaacttca gtcttagtgc
    15841 caatgacact tattctagtt actttaaaac ttaacccact tgtgattatg gctggcttta
    15901 ttgctgcagt tgttactctt aatattgctg actcgatacc gacagcaata acgtcgatcc
    15961 caggcggggt aatgtcaaca ccgcttgttg gtcatagtca aacactaaag aaacacggac
    16021 ttacttctta tagtgtacag tggatgatgc ttggttcttt aatcggtgtc ttagttagtt
    16081 tccccattgc cctagcgttt tggggtattt ttgaatttat ttctgtacaa actggattag
    16141 aaataagtaa ctttattaag cgttacaatg aatacatctt ccttggtggc gctattttga
    16201 tgagtttaac aagtaaggaa aaagttgttt cacttttatc aatcgttccg tttggatttg
    16261 ccactgcttt cttacgtaat ccaggtctta ttattaataa tacgctctta aatacgctta
    16321 tcggtggccg gactttatca atgacacctt tctttttaag tattacgact ggtccacttg
    16381 tgtttgcgtt atttgaactt ttcttcccca gtgtatttaa aaaccaaaag gttgaaggaa
    16441 agatgactgt tattcttgat gatgatgtaa agaaaacaaa gcatggtcat aaaaagatca
    16501 ttgctaaaac tggaatcgct agtgcgcttg gtagtttgct cttcttcatg tcaccggttg
    16561 gtgtcacaat gatgcttggt gaatttgcga cgaaagatga aaaagacgaa gtacaacaag
    16621 ctttacaagg tgtttctgct gttaacggtt tatcaagcgc tacttattta gcaggaatta
    16681 tgatttcact tttcgtcttc gctttcccta tttcaccagc ggcacttggt ccaggctccg
    16741 ttatttttag tcctggcggt ccaattattc ttgaaggtaa tgctgggtta gcggtaccaa
    16801 aaggttattt attaatcgcc attgttattg gtttagtaat tggacttagc ttaacagtgt
    16861 ggcttggtat gaaatattct cataaaatga ctgaacttgt atttaagtat atttcacaag
    16921 aaacagtgct tatctttttg ttctcaatta tcgcgcttct ttcttttgcc gaagctggtt
    16981 tatatggttt aataattgta gttagtgttg gccttctaag tggttggcta aataaaaaag
    17041 gagcgggtta cggtgtacag tttatggcac tttacgcagg tgggttattt attacaatcc
    17101 tcaaagcgct cttctaatta gggactaatt atggatggat acggtttagc aaaaattata
    17161 ttaattggtg agcattccgt cgtttacggt catcccgcta ttgcgattcc tttttatgca
    17221 atgcgaagcg aagttgtttt aactccgcac gaagagttta ttcttgatac gaagtattac
    17281 actggcccac ttaataatgc cgtagatgaa ttacacggac ttgttgcttt aataacacat
    17341 atggctgaaa tttataaaaa gattaagcct gtcattataa gtgttcgttc aagtttacca
    17401 gagaaaagcg gcttaggctc aagcgctagt gttgctaaag cggtaattga tgcttttaac
    17461 aatcattttc atcttgaatt aacacatatt gattactttg aattattaaa aatctctgaa
    17521 aatatatatc atcataacgc aagcggaatt gacgcttcta ctgttattta tgaaaagcca
    17581 attatatatc aaagaccgca cataaagcca ctgaaattta atattgatgg ctatttagcg
    17641 gtgttttatt caaatacaaa cagtgcgaca aaaagtgctg tcgcacatgt aagcactcat
    17701 cctgaacgcg acattcatat tgatgcttta ggttcattaa ctgcaaacgc taaatgggca
    17761 ttagaaaata atgacattac gcttcttgca acgatgttta acgctgctca cacacattta
    17821 agagcgctag gtatttcaac gcctacactt gaagaattac gcactaagct attagacgca
    17881 ggtgcgcttg gtgttaaaat tacgggtggg ggaatgggcg gatgtttaat tgccctattt
    17941 aatgatgaag aaaaagcact tgccactaaa gaaaaattaa agagctttcc atcatggatg
    18001 attgatttga ggaacatgtg atgaaatata aaaaagcaaa tgtaaatatc gctattctta
    18061 aatattgggg gaaagttaat gctttaatta atattcctta tcaaacaagt atttccgtta
    18121 ccgcggataa tttttatact ctcactaatg ttgaattaga tccaaaagcc actaaagata
    18181 ttgttattct tgatggtaca gcattaagcg gtatttctta tacaagagta gcccatcatt
    18241 tagacgcact ccgtaaacat tttaaacgta aagagtattg tattgttaat tcaatcaata
    18301 gagtttttac taaatcaggc tatgcttcaa gtgcgagcgg ttttgccgcg cttactgctg
    18361 cttatattga tgcaatcggt gcgaaagtaa gtaaaaaaga aatgtcgaaa ttagctaggc
    18421 ttggttcagg ctccgcgact cgttcaattc acggcggatt tgttatttgg catcgcggta
    18481 atagtcatga cacaagttat gctgaaaagt tacaagttaa atggccagaa tttagaatgc
    18541 tttttactat tattgatccg agtgttaaga aaatatcaag tagagttgga atgcaaatca
    18601 ccattgaaaa agctaagtct tacgctgatt atgtcaagca atcaaatgaa tctgtcgaac
    18661 cgcttattac tgcgcttaaa gaaaatgata ttgatgctgt tggtcgcatt gcggaacgcg
    18721 ctgctgaact tatgcgtaat gtaatgcttg aagcggggct tgaatatcat acaccaaaaa
    18781 cacaacaatt aattcaaaaa gttaaagcga ttcgctctaa acataaaatt cctgtttatt
    18841 atacgtttga cgctggtcca aacttaattc tttttacact tgatgaatat gttaatgaag
    18901 ttattaggct tttaccagaa gttaaaattt cagtaagtgg tgttggaggc ggtattacta
    18961 gtgcgcgttc atgaaaaagt accaggtaaa ctttatgtta tcggggaata caatgtttta
    19021 aaaagcggac atggtgcaat tgtagcgccc gttaatcttt ttttacaagg aacatttgtc
    19081 gctaatcata agacaacaat tattgaagat ggtgtcgctt atccctatca aacgttacta
    19141 gaaaatggct gtcctcatca attaaaaaat actgttacta cgattacatt ttttaatgaa
    19201 tatcttgctt taaaaaatat tcctttacag caatttgaat atactattga aaataattta
    19261 attagtgatg aacaagttaa gtttggttta ggatcaagcg cagcgagtgt tatcctaaca
    19321 cttaagttat taaatcgttt atatcgcact aatttgtcac cacttgtgct ttttaaaatg
    19381 gcagtcttaa tccagcgtga actaaatgcg cttacttctg gtggcgactt agcgtgtact
    19441 atttttgata tgccgcttta ttatgttcgt tatgatttaa attggttact taatgaaaaa
    19501 cgaacatttg aattacttga agttgcgtgg ccgcttttag aaattaaatt attaacaaaa
    19561 cttcctacct tccttgtggg ttggacaaaa gaaacaataa caccagacac taacggcact
    19621 attgattccg ctttctttaa aaaagcaaat aagcttgttc accattattt agttacttac
    19681 gatgatattt atataaaaca atatcaaaac ctacttgata aattaggatt aacgcgacaa
    19741 ggaatgatta ctccgcgttt agaagcatta gtgaaaactg cccgctcttt aggagtggcc
    19801 gctaaaatat caggggcagg atatggtgat tgcgggattg cccaaattaa tgaccagagt
    19861 gttgttgaac cattaacaaa tttatgggaa aatgaaggta tcatcgtact tgatatttgg
    19921 aggaaagata atgagtagac gccgtaaaga tgaacatatt gcgattgcaa aaatggctaa
    19981 gataagaaaa aatgatttta gtcacattcg ttttatccat catagtattc cttctttatc
    20041 attaaaagat atcgatcttt cgacttcatt tttaggacat aaatttaatt atccttttta
    20101 cattaatgca atgacaggcg gcgctaaaaa aggtgataaa ataaatgcta aattggcgat
    20161 attagcccag cattttggcc ttcctttgtt tgttggctca caaagtttag cgtttaaaaa
    20221 caaagaatca cgacttgcat ttgaaacact tagatttaac ttccctgatc tatttattgt
    20281 cgcaaatatt aaccccaatt tcactgcaga aatggctagt gatgcagtgg aaatcttggg
    20341 cgctaatgcg ttagcgattc atgttaatag tgtccaagaa ttagtgatgg gtgaagggga
    20401 tcgtgatttt acaaaatgga aagataatat tgctaacatt ttaaaaaccg ttaaagtacc
    20461 ggtaattgtt aaggaagtag gatacggaat gcatgaaccg actctcttac tcctccaaga
    20521 acttggtgtt aagcatattg atataagcgg aatgggtggt attaatttct ccttgattga
    20581 attaaaaaga caaaagaaaa aagattcacc acttcttgat tttggtattt ccactgttga
    20641 tgcattactc cttgcccgta attatccaag ctttgaagtg cttgcaagtg gcggtattaa
    20701 taacgctagt tcgattatta aatcactagc gatgggtgct aaagcggcag gtatggctaa
    20761 atatttcctc gatttatgta acttaccagt tcctgaaatg attaaaaaag ttgatgaatt
    20821 aattatcgac ttaaagaaaa ttttagtgtt attaaatgtt aagagtgttc atgaattaaa
    20881 tcttgatcac ttgatgtttg atttataatg gtttctttaa accataatgc ttaactggac
    20941 gccaggtaac tttcacgaat ggcgcaatta gtaacattgg tacatatgaa agcaagaaaa
    21001 agaatgctac aaacggtagc attatttttt taagtggatg cattttaatc cgtttccaat
    21061 ttgaaacact aattaataac gacattagtg tagtaaaaag ataaaaccct aataagaaat
    21121 caaataatgt aaaaagataa gtttgtataa aataattaat gtcttgagtg atcgctaagt
    21181 taatagcagc acctatccca tataatataa accaccaaat gatgtaagct ggaaacggag
    21241 tcgaaaaaag aaacgcttca taagaagaaa aacgctcttt tagtgtcctt ttgcgggcaa
    21301 aaatggactt aaataatgac caagtgtaaa cttttgaaac ttgaaataac ccttttaccc
    21361 aacgcatccg ttgatgccaa cttgatttaa agttaacagc ttgttcgtca tagaaaatcg
    21421 cgttatgtgc ataatgagtc cggccttttt ccatgacatg cgctgctgaa aactcaatgt
    21481 cttcaattaa taaatgatgg ttccagcctt caacttcttg cattttttcg taactaacaa
    21541 aaaaacctgt gcctgataca taagaactaa gacctaacac ctcacgacct ttatgcatta
    21601 agcaacattc acgtaaaaaa gagtaccccg ttccaaatgc ccaaagtgaa tcaccagcgt
    21661 ttgtactagc gcgataagtc gcgacaactt tagcgtttgt tgcttgatag actttattca
    21721 cttcttttag aaattgcgca tcaagcacat tatcggtatc aatcacaatg tagccggcaa
    21781 agtcttctgc gggaactact tttttttgct cattgaaata atattcaagt gcatagcttt
    21841 taccaatttt tgttaaatcg ttccgtgtat aaactttcgc ttttaactgc gcagcaataa
    21901 ttgcggtatt atcagtacaa ttatcggcga caacatggat gtttattttt tcttggggat
    21961 aatcaagcgc tctaattgtt gtgataagtt gcgcaatcac tgtttcttca ttccgcgcac
    22021 atgtcacgaa tgtatatgta ggaaatacat cggtttctgg atattttttg gcgcgaacaa
    22081 caacagaaaa gatacttagt agcacttgat agaaatataa aacactaaga aaggataaga
    22141 caattgtgtt taataaaact aatatatctc caagtgttga gcccggaatt tcttgtaatt
    22201 ccccaaataa tagttcgaaa aggctaagaa gaaaacgcaa ctgcgcaact aattttactt
    22261 tcataaaatt attatatctc ttttggggta aatactatcg aaaaatgata acatttagtt
    22321 gatattatga aagcaatgag aagactcgct aaagagcgca cgtatgaata cttaagacta
    22381 acttacttcg tgcgttttct cggcgatgct ttcttttata catttctgta ttttttttaa
    22441 catctcttgg ctttggcact gataaacttg gacttattgc tgctctttct ccatttgccg
    22501 cccttcttgg taatattatt tttcaacgaa tagcgaaaaa tttagaagtt aatcgtattt
    22561 taatgtttat cttttcaatc attgaaacga gcattgcgct tatcttcttt atttttccaa
    22621 agcaaccctt tatattttat gcaattatca ttacatgtgt aagtacgatt aatggtcctt
    22681 tttattcact gcttgacggt tatagcggaa catatatttc agagcgtaat aaacaatact
    22741 cttctatgcg ggtaatgggt actttatcat atgtcatcgg tccacttatt ggcgggtttt
    22801 tagttgataa catggtgctg gatttcaaac attatttctc atgtcatcag tgttttacat
    22861 cattgcttcc tttctaattt actatttacc aaagcaaaaa attgaattac atattattgt
    22921 tccgcctgaa gaaaaaaaga gacttaaaaa tattggaaaa cccgaactaa ttgcttattt
    22981 actctttaac ttcttcgtca ttggggttac tgttgttacc gataactttt ttggtattta
    23041 tttaaggact gaattaggag ttacgacaac acagtttgga tatattgtgg cgattgcaat
    23101 agcaattgaa gcaggcgtga tgtttttact tgcactacga aaaaataata gtttccgagt
    23161 gccagccttt tcgtttttct ttatcggaac atttttgctt attcgtccgt tagtgattgt
    23221 tcttaattta tcgccgacat tgacaatgat attgtcagtc tttcgcggga ttgcatgggg
    23281 atattacatc gtttttaatg tgcgcttttt ggctcgtgtt atccccctaa agcacttaac
    23341 aaaggcactt cttactggat caattgccac aacttcaagt cgcattatta ctagtttaat
    23401 cgttggcgaa agtcttaaac atcattctta tcaaacggtg tttttctttt caatgttctc
    23461 tgtagttttt gggatgacaa ttagtattat tattgccgaa attgatcggc gaaaacaaaa
    23521 aaaggtcgat atgatcgtac cttttattgg ttcagataaa gatttaaatt aatctagggt
    23581 atccactaat ttgatggagt aactgatgac tttgttctcg gcagtaccgg taacatcgtc
    23641 atcattttca aaaacaaaac tggcgcggaa cttttgaccg ctaagtgcta gatgcaacca
    23701 atatttatca tcatcccaca tttcatcaac tggcacttca ttaaaaccaa accatcttgg
    23761 taccatttca tcagattcgg tgatttcacc gctccattca gtacatacat ataaatgcat
    23821 ccgtaatgga tagctttccg caaaatcaat ttcagcggct ttataatatg ttttcattgt
    23881 aagacccgct tcttctttag cttctctaat cgcagccatt tcaattgttt caccactctt
    23941 taatttacca ccaaagccgt tatatttctt tttaccaaaa ccgcgttttt tataaccaag
    24001 taaaaccttg ttgtttttta taaataatac gtttgtaaca tctctaattt ccatattttt
    24061 ttgtctcttt tcaataatag tgtgttaagc acattgctta atatacgcta ataaggggga
    24121 aataacaaga aaaaggaata aaaaaaacac accagaggtg tgttccttta acttcactaa
    24181 ttttaattaa ttaggcaagt ttgtagtctc tgtggatgct aagcatcact aagtcgtaaa
    24241 tccagccaat aacgaaaaat ccgccagtga gtagataaat gacggcccaa aggtcacgtc
    24301 cgcgaactaa gcgaataact gctccccaaa taggtccaat gaaaaattgg aggagtaagt
    24361 gagcaatcca tggtaatcca aattctttaa ccattttgta cctcctatgg tttatgattt
    24421 tatgatatat caattaatat aaaatttcaa tctaataaat gtaaataata gaagaaaa
//
LOCUS       DAOSTA010000014        24090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_2272_length_24090_cov_8.601706, whole genome shotgun sequence.
ACCESSION   DAOSTA010000014 DAOSTA010000000
VERSION     DAOSTA010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 24090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24090
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2272_length_24090_cov_8.601706"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..163)
                     /locus_tag="PLU08_02370"
     tRNA            complement(90..163)
                     /locus_tag="PLU08_02370"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(129..131),aa:Gly,seq:tcc)
     gene            complement(173..248)
                     /locus_tag="PLU08_02375"
     tRNA            complement(173..248)
                     /locus_tag="PLU08_02375"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(213..215),aa:Asp,seq:gtc)
     gene            complement(353..1015)
                     /locus_tag="PLU08_02380"
     CDS             complement(353..1015)
                     /locus_tag="PLU08_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOQ70582.1"
                     /translation="MANELDELTGPVNEEGQDINVISKQLKVKVGWGSTLFEVLLWFP
                     LIIPGVIFLFKKIQAKKHFQQLQQRIQHHASQIDNYLEQRVVILNNVVGLVNRAVALD
                     KDTFTTIAAARSGASLDENRNVVAGQVGDVTNKLNIAFENYPELKAHQAIQDAMQQNS
                     YLQREITAARELYNDVVNRWNSDVYAWPTKMIVAAREGYTTRIPFSASAEIKAEARKT
                     FF"
     gene            complement(1062..2909)
                     /locus_tag="PLU08_02385"
     CDS             complement(1062..2909)
                     /locus_tag="PLU08_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70583.1"
                     /translation="MSKLIFDPLEEYRDKFKGLHDQNVNRFFDELVQKSGINIPENDL
                     TVKEIDNLNIKIDNEEKVLRRVRRIRGFLLFLFIAALIVFILFIVAAVQGEESSLTMS
                     VGARVGIAIGVIVFGIAMLIIVIKVLRPRIKDSQARLSDLISKRDEKIRLAWAQVNPL
                     NELFDANMTPMLVEKTIPLLTMDPFFDSKRLDYLVRKYELSDFTPDNESAEFVQSGEI
                     QGNPFILARTLVMNMGTKTYTGTLTVSYTVTVYVNGKAQRQTRTETLRASVNKPCPYY
                     NNDTYIIYGNEAAPNLIFSRYPQLPLDWTEKSLRCFVEKEEKKMKKLSEKALKQGKQF
                     TPLGNTEFEALFNAYDRNNELEYRLLFTALGQSEILDLIKDKKVGFGDNFQFFKNKNL
                     NLVRSKHSQAFDYSGNPRNYYHYDNKEIRKRFVDYNNNYLHRFYFNIAPVLAIPLYQQ
                     HKPHEFIYQTEYKSHLSFYEHESTVNQFPSSAFAHPDSHTQNILKTKVVQKGKDFDTV
                     SVTSYGFTTVERVDYVPKMAGNGTIHNVPVHWTEYIPVEKESHATIKVLDEESVKKTQ
                     NESFFDKIGEYLSKYTPDQKPVTGRHVMAFLVASKFADDDNEGLDKLFK"
     gene            complement(2970..3278)
                     /gene="trxA"
                     /locus_tag="PLU08_02390"
     CDS             complement(2970..3278)
                     /gene="trxA"
                     /locus_tag="PLU08_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009872735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOQ70584.1"
                     /translation="MIKYLENAADFKEIISKGTVLVDFYADWCGPCRMLGPVLEKLSK
                     ERTDVTVLKVDVDTFGALSQQFSVFSIPTMILFKDGKPVGKQVGYKAFSQLVDFIDNN
                     "
     gene            complement(3288..4118)
                     /locus_tag="PLU08_02395"
     CDS             complement(3288..4118)
                     /locus_tag="PLU08_02395"
                     /inference="COORDINATES: protein motif:HMM:NF017655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HOQ70585.1"
                     /translation="MQANRHLILNSFTLKIIAMSAVLIDHVALLFINPTLTIYILMRI
                     IGRIAFVLYAFFISEGVIHTKNTNKYLLRILYLYLIIQALIIGVLIYDPVIELRNIFA
                     TLGAGASLLIYFEKKEWKKVYYLLPFIIVSTINLLAYFTKDSWFIAFSGDNGFFGLAL
                     IIAFYVARKFSIYLLKKYPPSDNTAGEDAEKSAQHVYNVASCVSLLFVASIWYILSVY
                     NSEIPHVALQSFSLISIPLLLLYNGKLGHNSRSWRIIYYSFYPVHVVMLALLFFIIKL
                     "
     gene            complement(4105..4929)
                     /locus_tag="PLU08_02400"
     CDS             complement(4105..4929)
                     /locus_tag="PLU08_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOQ70586.1"
                     /translation="MEEQEKRVDEVNQSEVEVMLEPLNYGSNHHEESRIKSPGFGYGL
                     FFSSLILLFLASFTIDFIVDALVGEGNEVTAFAYKNFGTYVVTFSLITVFLFLTKTLL
                     PLVKKFTKIRPYIHGLFYGFIVIIISVITTLLMQILFGQAEENLNQQIINLILSKHPV
                     SSFFWIVLLGPLVEELTYRVGLFNAISSRNRIAAYVVSALVFGFLHFNIPLNADGAID
                     QAKLIEEFINIPSYIVSGLVFGYIYEKEGFAGSSVAHITNNLISYILSIISIYASK"
     gene            5006..5608
                     /locus_tag="PLU08_02405"
     CDS             5006..5608
                     /locus_tag="PLU08_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOQ70587.1"
                     /translation="MGYVINKSYINEIVVEKSRFIALLCPLTNENDFDEYFKESKTTY
                     KDARHYTYAFRYGNLERASDDGEPQNTAGLPLLNLLKNHDLVNTALIVIRYFGGKKLG
                     AGRLLRTYVDAGNRVINIADKFLEIPAFKYEITIPISDFERTRHYLEKDGFMIDDTDF
                     SSLNVKVLVRVSKDQSESFENLYNVVNKSESVMLKECNDE"
     gene            5601..6365
                     /locus_tag="PLU08_02410"
     CDS             5601..6365
                     /locus_tag="PLU08_02410"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOQ70588.1"
                     /translation="MNKIKKENIKHIIGIVSGKGGVGKSYVTGSIAVELAKQGYRVGI
                     LDGDVTGPSIPYMFNITSGVYGDEEIIYPATTEKLGIKIISSALLLAHGEDPIMWRGP
                     LIGSLVTQFYTTVNWGELDFLLIDFPPGTSDVALSAFQTIPVDGIVLVTTPQQMVQQI
                     VKKTYKMGEEMKVKLLGVIENMAYVKLPNSDEKYYLYGQNKLEDLQNTFPINILGSLP
                     FLPQNTKLVDEGKIEVTDTTEFSAIVEKLIKELQNE"
     gene            6358..7569
                     /locus_tag="PLU08_02415"
     CDS             6358..7569
                     /locus_tag="PLU08_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HOQ70589.1"
                     /translation="MNKYSNRRQKLYKELANNSALILFSGCEIKKSADGFYDFDVNKN
                     FFYLTGIKQEGSILVVTKIKNIVKETILTSPYDAHKEIWTGRRLKPENVREITGINEY
                     KTTDEFNALLQTLKGNVKTVYLDLEPGIFTHTGLLVSNFREEIINTVNVKVENVYPLI
                     VRLRMVKDEEEIELIRESIALTKKALDFAISKVKPGAYEYEVAAAFEYKIKAAHGVLG
                     FSTIAATGDNAVILHYPDLNTKLEAGELLLLDLGGHNNMYTADISRTVPVNGKFTPLQ
                     ITIYEIVLACNKAVIDFIKPGKTIADLQNYTREFLKERLVKAKLMDKDEDISNYYYHG
                     VSHHLGLDTHDPADRALPLEKGNVITVEPGLYFAKYGIGVRIEDNVLVTEKGSENLSI
                     SIPKEIKEIIS"
     gene            7569..9053
                     /gene="glpK"
                     /locus_tag="PLU08_02420"
     CDS             7569..9053
                     /gene="glpK"
                     /locus_tag="PLU08_02420"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOQ70590.1"
                     /translation="MAQYILTIDQGTTSTRAMLFDKNAEIAFIAQEEIKCYYPKPGYV
                     EQDAIELWLSVLSVINQVLIKANLTFHNITAMGITNQRETTIVWDKNTGMPVAPAIVW
                     QSRQSADICNALSKHQNMIHKKTGLLINPYFSASKIRYILDNLPNGQERADAGELMFG
                     TVDSWLIYKLTNETRHVTDITNASRTMLFNINTLEWDDDILKLFNIPRSMLPEVLASA
                     DYFGETSYFHNTIPITGVAGDQQAALFGQMCLKPGDAKNTYGTGCFMLMNIGDKVQLS
                     KNGLLTTVAWKINGKVTYALEGSVFMGGASVQWLRDQLKLFKNAADTENMAYKAQQND
                     SIYFVPAFVGLGTPYWDDNARGAVFGLTREAGRHELARATLEAIAYQSRDVFGVMIKE
                     SKQDLKVLSVDGGASVNNYLMQFQADILNTTIRVPKIYETTSLGVCYLAGLHTGFFSS
                     IEAITKIHEYTRTYEPKMEEKVRERKYAGWKKAVRAVLTFSEEE"
     gene            9183..11510
                     /locus_tag="PLU08_02425"
     CDS             9183..11510
                     /locus_tag="PLU08_02425"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOQ70591.1"
                     /translation="MNDLVKRLKGYDYVIVDYATKLELLKLRIRNPLLTFKIFTVEEL
                     ITKFLGSASDLSLFALLEKYNNLTYEHAKIINNTIVFPNVSKSSLKVNTLSTYQKALK
                     ALNLLESDFLIAHSLHNKKILLPKYLASNYLLINLLNSVHANFEFTEDMHNFIHEITV
                     FHTGVDEVYAALNRIAALLANGVKPSFIKILKPNSAYEELLRKLAPQFNIVLGPRLFP
                     LNAFSDVVAFVEQFKGQRIDISDPLIQTLRMSTSAPLIALYNLIIAIDFNSVSNKHHY
                     AILKDKIKSESIALEDNDGIEFINEIPLFSANDTYYFYLNFANGAAPNIQSLNPYLNN
                     EERKQLGLLDDETIALINENKLISTLNNNKNIYISFAKIFGQENYELSTLADKLEYEC
                     IEHQLAHKIYSQKYFNYLTGVSYDKLIFYKKHDENFDELFIANDYFDDYGSFDYKFKG
                     INHYHEMVYLSPTKIDLYQRLPFDYFVQHLLNIVDDRPSIHLHFGEFVHSVLENSTSE
                     ETFKVCFSHYLDQMNFNTKDRFFVENERDLIAEAFRFNQRYLNTVMPTKIHNETLIYA
                     PFAAKCVLNGRLDRILLFEKDEKKYIFVVDFKTGNATTNPKNYEYGLDLQLPLYGLLL
                     SLTEDYKDYEVSALVLNSIKLTSTYSAGSRETFTEAIINDIKYTGILIDDADSLKILN
                     AEADSERPFFKIGGRGKVKKLTDLIRREEYKNYMEVTRNKVEETYVGILNNDFRIEDK
                     KINNESSAKYSRYKEISFLPRNSVIEEADYEEGNE"
     gene            11491..14616
                     /locus_tag="PLU08_02430"
     CDS             11491..14616
                     /locus_tag="PLU08_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOQ70592.1"
                     /translation="MKKETNKNTITFSPKQEEALSLNKENILVSAGAGSGKTAVLVER
                     IIRILIDKEVPLERILVLTFTRPGAQEFKNRIKKSLLENKTHAHKAFAVDNADITTFD
                     AYSLKILRKYGYLHPTMQSDITNLDANFEMVLMKQYFDEIMAEYYENPPLSLRYFATR
                     FLSKDDRQLFELIKSLDQQVNLQIDPEAYLDNYLDSFYSDTKFEEIYKELDAYVVTTI
                     RKVANAIKHFECERHLEAYETFLDHYGAILSFKDYMDNLDKFTYQRRPSGSLKEHPED
                     LPHAEEVKIYLNDLKEVQVFESKDRMYDVFSENKGHAAFVIEIYSQIRAKLAAYKKEK
                     QAYTFSDIARGAFELVSIPEIQHELKNQYDYILIDEYQDTSDIQEQFINKIANNNVYM
                     VGDIKQSIYGFRNANCDIFREKYARYRKNEGGKKIDLNDNYRSRREVLQSINAILSEI
                     MTEEYGGANYKKEHVINSGNLKYDEFKEERQKVGLEVINYVVPKKNKKNRDNNVLIRD
                     KIDYELDYIINDIEHKIVNKYQVYENKVGMRDVRYGDFTILIAQGTKFNRIEERFIRA
                     GIPVYVDRDEEANENNINIAINKLLTIYVAYTKSDEPKGFEFRLAVASFLRGFVNKYS
                     DQQLFDLLKDDNWDFSGDPILARIKKLAQTTKLLPVTEIIERLITEFDVYGALYNIRD
                     LTQNTMKLSSKLEEIENLAALGLDLEAIVNYFKEREIAGVNAALKRVKLEHDAVTIMT
                     IHRSKGLEFPIVYYMDLESNFYQVSRSQVMKVCPKYGFILPEPSYKSQKTFIGFLSNI
                     YTSKETLSEKIRLLYVALTRAKELMVVIHSTNEDGEPYKTFEKSSNLKQLLLQSTYYD
                     QKRLVVEPEYTQQRLSESEHSKEVKYIYSLHEHFYKFNKKPKTYTVSEIVDVDERLLA
                     RGTLLHRYFEEYDFTTKDLNYISDERDRQHMLNLLSLPFFANANANNIFREYEFIDDG
                     TNKTYAIDCFIIQDDKIMLFDFKLSNIYHPSYDVQVRNYAHYLQKVFKKSVEGYLISI
                     TDLKVRKVSNE"
     gene            14609..15697
                     /locus_tag="PLU08_02435"
     CDS             14609..15697
                     /locus_tag="PLU08_02435"
                     /inference="COORDINATES: protein motif:HMM:NF039499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HOQ70593.1"
                     /translation="MNDKKTYTPISNENFLRLLRFYKIPESAEDEVLYNLYIETVELL
                     TLHHQTFENIPYINLDHQRLILQLIHDYDFRMRGLNFEERRSLLKDELFHNKLINVVV
                     DKYGSSAIFKYDSGTYLTPFSMEISTINVYLNFIMLKLGSIPRHNKATELYAELLTSA
                     FSYVLTITELLVRGFEKEALATWRSLHELEATLLLIQDEKVLAQYNQHILYALAFNKL
                     IAQAESDKVFIEIKAKMKDLKLKSKDTKRFIEYGWLLAHNDFDLNIHKFNFRDGVQTL
                     AGLNHKRDIYQIASEVTHSSPLTLFTKRHYFLSIALENLYSSFLTIEVLFAAFYIKNT
                     TKNEAEFYEVTRSIYLEDINFVKDRITK"
     gene            complement(15748..17406)
                     /locus_tag="PLU08_02440"
     CDS             complement(15748..17406)
                     /locus_tag="PLU08_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOQ70594.1"
                     /translation="MENKKTKTFTVVYNGKTTTHDEPVRILDIVGPDKNLICAYVNGR
                     VRELTFLLNRDAILETKTLDNGEGIRIYQTSLRFVAAMAMKRIRPELNVRFSYNVSRS
                     IFIQITNKGVTVDRLLVEKLQREMRNIVEADLPFNRTKITIEEAEKLYEELGYKDKLE
                     ILKYRPEPTVHIYECDGYENYMYGYMVPSSGYLKNFIIRPYSPGMLLQYPRSETKGEI
                     PEFKDAPMFGKALKASHNWAKLTNLDSVAHINHYIENGHIVELINIAEAKHYQMLSEL
                     GQRILEDSEDIRLICIAGPSSSGKTTFANRLRIELMSRGLNAIRLSIDDYYLPRELAP
                     RDEYGNYDLESIYALDIELFNKNMLDLINGEEVELPKFDFKKGQRVPGRKLKVAANQP
                     IIIEGIHALNEQLTSYIPKHQKYKIYIAPQAQINIDEHNPISLTDLRLLRRIVRDKKY
                     RNASAKETMAMWPSVRAGEFKWIYDTQEEADYVYNSLLSYELAVMKKHALPLLLEFDY
                     LSEFFPIAERLIRMLKYFLDMDEKWVPSNSLLREFIGGSCFEDV"
     gene            complement(17498..18256)
                     /locus_tag="PLU08_02445"
     CDS             complement(17498..18256)
                     /locus_tag="PLU08_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOQ70595.1"
                     /translation="MKKEKILQLLLFIYIFSSFLSGCKVSNPNQILVPKRLETTITSE
                     NYTYNMTKAYHEAFTKGKKSFVLYFGSHRCQYCAEIEPLLLNYVQKTKIEIYYIDLYS
                     QEYGDNREYYIETTNVMGAPNILIYKEGEMVHRERGASNLSTQLEVNKFFKEHVRTNN
                     YYTTDKKDNIKPDFKKYVTFTYNFNNATHHELINTKFFPYFEKKGYVNYLVNEDTPLD
                     TPTLSYGSSNKEISLPNDDTELETIISNYESYFS"
     gene            18323..18868
                     /locus_tag="PLU08_02450"
     CDS             18323..18868
                     /locus_tag="PLU08_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOQ70596.1"
                     /translation="MNIWHDMDPRRITTERFDAVVEISRGQQSKYELDKETGLIRLDR
                     VLYTSTHYPQNYGFIPRTYAGDDDPLDVLILCSQPLVPMTIVTCTPIGAVRMLDGGEL
                     DEKIIAIAANDPTYSSYASVDEIPQHVLQEIRHFFQVYKTLEGKETVVHHIQGRDKAI
                     DIIEKSIEMYNEVFNIKQAEE"
     gene            18868..19371
                     /locus_tag="PLU08_02455"
     CDS             18868..19371
                     /locus_tag="PLU08_02455"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009289280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOQ70597.1"
                     /translation="MLNIILYQPEMPANVGNIMRTAVAIDATLHIIGPTPIKLDDKSL
                     KRAGMDYVQDLSFFIYDSIDKFMEKHHDKNIFFVTRYSKNNYTEVDYGRFDAGIYVMF
                     GNESYGLPMELLRKHKENTLRIPMVATARSLNLSNAVAVVSYEILRQQGFYNLATSEV
                     IKGDDYL"
     gene            complement(19362..20738)
                     /locus_tag="PLU08_02460"
     CDS             complement(19362..20738)
                     /locus_tag="PLU08_02460"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HOQ70598.1"
                     /translation="MQRLFPNDLQQLINANKHFEGLFLVRIRQLSLVDFVFEFSHGNN
                     LFVSLNSPTPFIVFNADLSDFPEGGELHSFAINLRQFLGGNLVAMNQPNDDMILSLVF
                     EQRNNVLELEEVTLFIELIPRHPQAVLVNNKGRILAAYRYHQERAKDGRFIRGGSKYS
                     LPEKGAFSPQISNAKTDYLQAYLKENRERIKKQNFRDVYVYLETNIKRSKRLIRNYEQ
                     DLEKLKELPDLYHKANMLLTYKPAISYYFVQIEGETIEVDPRFDALFNAEILFKKAKK
                     IKKSENILMSKIAETKEKIKYLESIENHLKSLESAGEIFQIYEELGLLKRRDKRQIIS
                     KLNPYVITYKDIDILFGKNNKQNDFLTFKIAKKNDTFMHIQNRPGAHIIIRQENPAKD
                     VLEFAGMLCLFLAKQIDGEVMYSPIYNIKKGAFPGQVLTRKYNTFFLRFNDDLKPFFE
                     NNIKRFYK"
     gene            20827..21399
                     /gene="gmk"
                     /locus_tag="PLU08_02465"
     CDS             20827..21399
                     /gene="gmk"
                     /locus_tag="PLU08_02465"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOQ70599.1"
                     /translation="MRQGFIVLISGPSGVGKGTLIHSLLADQTLNLSFSVSMTTRKPR
                     PNEKDGIHYFFVSHETFLKNIEENNFLEHAQFVGNRYGTPRDYVEKLLNEGKNVLIEI
                     EVDGARQLLKNVDSEKVLSLYIMPPSVQELVRRLRLRGSEDEATIQRRAERFKEEIKY
                     KDQYQHIIINDELETCINDVKALIREKMNI"
     gene            21402..23591
                     /gene="priA"
                     /locus_tag="PLU08_02470"
     CDS             21402..23591
                     /gene="priA"
                     /locus_tag="PLU08_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOQ70600.1"
                     /translation="MQLIEVFTQYSKLNRPFTYYYDGDDLEVGVRVLIDFNNREIVGY
                     VAAVKSTKETIETASKRLGFELAEITKILDEKPLLTNELMELSENVTSYYLTNRISVL
                     NAMLPPSLRPSKGSLSAPKIAMQKKVSFNPEFTDFDKLTDKQSSLLSEIMTHGSVLQT
                     SKNRTQIMQLLNKGAVIVEEIEKYRLRYEDYPREDPHELTNLQKEAVKSILEDYKETF
                     LLHGITGSGKTEIYLALAAHYLAQNKQVIMLVPEIALTGTMLANFYRRFGKKIAILHS
                     ELTPAEKYDEYRRISAGEVDIVVGARSAIFAPLQNIGLIILDEEHAETYKQDVAPFYH
                     ALTVAQMRAKSFNAKVLVGSATPSIETMARARKEVYGYYYLGERINLQPLPETTVVNM
                     LDYKNVTTTSPYISKLLATEIRKAIDAKTQVILLVNRRGYATFVSCRECGESKKCPAC
                     AISLTYHKVGNNLKCHHCGLSFPKNDPCIKCGSTNFSYTGFATQKAEEDIQKMFPDAR
                     ILRLDSDVSRKDKTPQVVLNKFMNHEADILIGTQMVSKGHDFPLVSLVGVIGTDANLS
                     IPSFRSSERTFQLITQAIGRSGREKIKGKAIIQTMMPEHYVIQHAKQQDYFGFYEVEI
                     RHRYLTSNPPFYFLLSITMSHQNERTVKDTILTLKLNLIKALGEDATVIGPTATFYPA
                     SGKTFNETLIVRYKNYFKIKPALLTTLSPFREQSSLYVHINVDPYDV"
     gene            23606..24046
                     /locus_tag="PLU08_02475"
     CDS             23606..24046
                     /locus_tag="PLU08_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70601.1"
                     /translation="MVIIKIYSQDIYVAAEISVKYADAIAKTLGLTKEDVFLLGSDGA
                     LIAEGVDQVSWFTYLEVTIPANLKHKQDALSKTLLEIFNNYGVHLMIKYDYVTKDNIV
                     KILNPDFKVFNETMVEVLPTGNYYDEDDVDWDDDWDEDEDKEVR"
BASE COUNT         8251 a         3808 c         4238 g         7793 t
ORIGIN      
        1 gcctcagaaa cacgaattct tctaaggctt ataaatttct ataatcgctt tgttggctta
       61 ataaagccct tactaaagta agattttttt ggagcaggtg acgggaatcg aacccgcgta
      121 tttagcttgg aaggctaatg ttctaccatt gaactacacc tgcatacgtc actggcggac
      181 cagacgggac tcgaacccgc gatcttctgc gtgacaggca gacatgttaa ccactacact
      241 actggtccat tgctttacta ttatattaaa attacgatta atatcaagtc ttattattta
      301 gaaaaagaaa aaccaacggt taggttggtt tttcctatct acaagattct ttctagaaga
      361 aagttttacg agcttcagct ttaatttctg cagatgctga gaaaggaata cgagttgtat
      421 aaccttcgcg cgcggcaaca atcatctttg ttggccatgc gtaaacatca ctattccagc
      481 gattgacgac atcgttataa agttcacgag cggctgtaat ttcgcgttgg agatagctgt
      541 tttgttgcat tgcgtcttga attgcttgat gggctttgag ttctggatag ttttcaaaag
      601 cgatgttaag tttattagtg acatcaccaa cttgtccggc gacaacatta cgattttcat
      661 caaggctagc accactacga gcagcggcaa ttgtggtaaa ggtatctttg tcaagtgcga
      721 cagcgcgatt aactaaacca acaacgttat taagaataac gacgcgttgt tctaagtagt
      781 tgtcgatttg actagcgtga tgttgaatgc gttgttgtaa ttgttggaag tgttttttgg
      841 cttggatttt cttaaataag aagataactc caggaataat aagtgggaac caaagtaaca
      901 cttcaaagag tgttgaaccc catccaactt ttacttttag ttgtttacta attacattaa
      961 tatcttgacc ttcttcatta actggtccag ttagttcatc taattcattt gccatagtat
     1021 ttttctcctt tctttaatta tactattaat aaataaactt attacttaaa taatttgtca
     1081 aggccttcat tatcgtcatc agcgaattta cttgccacaa ggaaagccat aacatggcgg
     1141 ccggtgaccg gtttttgatc aggggtgtat ttacttaaat attcaccaat cttatcaaag
     1201 aatgattcat tttgtgtttt cttcacgctc tcttcatcaa gtactttaat cgttgcgtgt
     1261 gattcttttt caactgggat atattcagtc caatggacag ggacattatg aatagtgcca
     1321 tttcctgcca ttttgggaac atagtcaacg cgttctacag tagtaaaacc gtagctagtc
     1381 acactgacgg tatcgaagtc tttacctttt tgcacaactt ttgtttttaa gatgttttgt
     1441 gtatgactat cagggtgcgc gaaagcacta cttgggaact ggttcaccgt tgattcatgt
     1501 tcataaaatg aaaggtgcga tttatattct gtttgataaa taaattcatg tggtttatgt
     1561 tgttgataaa gcgggattgc aaggacagga gcgatattaa aatagaaacg atgcaaataa
     1621 ttattgttat aatcgacaaa gcgttttcta atttctttat tatcatagtg ataataatta
     1681 cgtggattcc cactataatc aaaggcttgt gaatgtttag aacgaacaag atttaagttt
     1741 ttgtttttaa agaattggaa gttatcacca aacccaactt tcttatcctt aattaaatct
     1801 aatatttcac tttgacctaa agcagtaaat aataagcgat attctaattc attgttgcgg
     1861 tcataagcgt taaagagtgc ttcaaattca gtattaccaa gtggtgtaaa ttgtttacct
     1921 tgttttagtg ctttttcact aagttttttc atcttttttt cttctttttc gacaaagcat
     1981 cgtaaactct tttctgtcca atcaagtggg agttgcggat aacgactaaa gattaaatta
     2041 ggcgctgctt cgttaccata aataatataa gtatcattgt tgtagtatgg acacggttta
     2101 ttcacacttg cgcgcaatgt ttctgtgcgt gtttgtcgtt gcgctttacc gttaacatag
     2161 acggtaaccg tataagaaac agtaagtgtc ccagtataag ttttagtacc catattcatc
     2221 acaagcgttc ttgctaaaat aaatggatta ccttgaattt cacccgattg cacaaattcc
     2281 gcggattcat tatcaggagt aaagtcactt aattcgtatt tacgcactaa gtaatcaagc
     2341 cgcttggaat caaaaaatgg atccattgta agcagcggaa ttgttttttc cactaacatt
     2401 ggtgtcatat ttgcgtcaaa cagttcatta agcggattaa cttgtgccca tgctaggcga
     2461 attttttcgt cacgcttact aattaagtca cttagtctag cttgtgaatc tttaatccgt
     2521 ggtcgtaata ctttgatgac aattatgagc atagcgatac caaatacgat tacgccaatg
     2581 gcaatcccta ctcgtgcacc aacgctcatc gttagggaac tctcttcacc ttgcactgcg
     2641 gcaacaataa ataaaataaa aactattaaa gcggcgataa ataaaaataa gagaaacccg
     2701 cgtatacgcc gtacgcgacg gagtactttc tcttcattat cgattttgat atttaaatta
     2761 tcgatttctt taacggttaa atcgttctca ggaatattaa tgccgctttt ttgcactagt
     2821 tcatcaaaaa aacggtttac attttggtca tgtaaccctt taaacttgtc acgatattct
     2881 tcaagtgggt caaaaattaa tttgctcata gattgattat aacaaagatt aaaaaattgt
     2941 aatttatttt atgataaaaa tttcaaattt tagttattat caataaagtc cactaattgt
     3001 gaaaaggctt tataaccaac ttgtttacca accggtttgc catctttaaa aagaatcatt
     3061 gttggaatcg aaaaaacact aaattgttgg gagagtgctc caaaagtgtc aacatcaacc
     3121 tttaaaacag tgacatcagt gcgttcctta cttaattttt caaggactgg accaagcatt
     3181 cggcacggac cacaccaatc agcgtaaaag tcgactaaga ctgttccttt actaataatt
     3241 tctttaaaat ctgctgcatt ttccaagtat ttaatcataa gtgcctccta gagtttaata
     3301 atgaagaata gtagtgcaag cattactaca tgaacgggat agaaagaata atagataatt
     3361 cgccaactac gactattatg gcctaattta ccgttataga gtaacaagag cggaatactt
     3421 ataagtgaga aactttgcaa tgcaacatgc ggtatttcgc tattgtacac gcttaaaata
     3481 taccaaatgg acgcaacgaa taaaagagaa acgcaacttg cgacattata cacatgctgt
     3541 gcgctttttt cagcatcctc tccagcggta ttatcactag gcggatactt cttcagtaaa
     3601 tagatactga atttacgcgc gacatagaaa gcgatgatta aggctaaacc aaagaagccg
     3661 ttatccccgg aaaaagcaat aaaccacgaa tctttagtaa aataggctaa taaattaatt
     3721 gtcgaaacaa taataaacgg taataagtaa tacactttct tccattcttt tttctcgaaa
     3781 tagatgagta aacttgcacc agcgccaagc gtagcgaaaa tatttcttag ttcgataact
     3841 gggtcgtaaa ttaaaacgcc aatgataagt gcttggatta ttaaatataa atataaaatg
     3901 cgcaataagt atttattggt atttttagta tgtatcactc cttcacttat aaaaaaagcg
     3961 taaagtacaa atgcaatgcg cccaataatg cgcattagga tataaattgt taatgtggga
     4021 ttaataaaaa gaagcgcaac atggtcaatt aaaactgcac tcattgcgat aattttaagc
     4081 gtgaatgaat ttaatattaa atgtctattt gcttgcataa atactaataa ttgaaagaat
     4141 atacgatatt aaattgttag tgatatgggc aacacttgac ccagcaaacc cttctttttc
     4201 ataaatatag ccaaagacga gtccgctaac aatgtaacta ggaatattaa taaattcttc
     4261 aattagttta gcttgatcaa tagcgccatc agcgttaagt ggaatattaa agtgtaagaa
     4321 accaaacaca agtgcgctaa cgacgtaggc tgcgatacga ttccgtgacg atatcgcatt
     4381 aaataaccca acgcgatacg ttaactcttc cactagtggg ccaagtaaga caatccagaa
     4441 aaaggaactg acaggatgct tcgataagat taaattaatt atttgttgat ttaagttttc
     4501 ttcggcttga ccgaatagaa tttgcattaa aagtgttgtg attactgaaa taattatcac
     4561 aataaatcca taaaataaac cgtgtatgta tgggcgtatt ttagtaaact tcttaacaag
     4621 aggcaaaagt gttttagtta agaataaaaa aactgtaatt agcgaaaatg taacaacata
     4681 ggtaccaaaa ttcttataag caaacgctgt tacctcattt ccttcaccga ctaaggcatc
     4741 gacaataaaa tcgatagtaa aactagcgag gaataaaagt ataaggctac taaaaaataa
     4801 tccataacca aaacccggtg atttgattcg tgattcttcg tggtgattac ttccataatt
     4861 aagtggttca agcatgactt caacttcact ttgatttact tcatcgactc ttttttcttg
     4921 ttcttccata cataaagatt ataacattta cttatttttt gcgttagtaa agcataaata
     4981 tcataattct tgatagaatt aaactatggg ttatgtcata aataaaagct atattaacga
     5041 aatcgttgtc gagaaatcac gcttcattgc gcttttatgt ccgctgacta atgagaatga
     5101 ttttgatgaa tactttaaag aatcaaaaac aacatataaa gatgctagac attatactta
     5161 tgcttttagg tatgggaatt tagagcgcgc tagtgatgat ggcgaaccac aaaacacagc
     5221 aggattaccg ctgctcaact tactaaagaa tcatgattta gtgaatacag cgcttattgt
     5281 aatccgttat ttcggtggta aaaaacttgg agcaggtcgt ttactgcgca catatgttga
     5341 tgctgggaat cgcgtgatta atattgctga caagttttta gaaattcctg cttttaagta
     5401 tgaaataact ataccaatta gtgactttga aaggacacgt cattatttag aaaaagacgg
     5461 gtttatgatt gatgacactg attttagtag cttaaatgta aaagtgctag ttcgtgttag
     5521 taaagaccaa agtgaatcgt ttgaaaatct ttataatgtc gttaacaaaa gtgaaagtgt
     5581 tatgttaaag gagtgtaatg atgaataaga ttaaaaaaga aaatattaaa catatcatcg
     5641 gtattgtttc tggtaaaggt ggtgtaggta agagctatgt aacgggttca attgctgttg
     5701 aactcgcgaa gcaaggctat cgtgttggaa ttttagatgg tgacgttaca ggtccctcaa
     5761 tcccttatat gtttaacatc actagcgggg tttatggtga cgaagaaatt atttatcctg
     5821 ctacgactga aaaattagga attaagatta tttcaagcgc tttgcttctt gcgcatggtg
     5881 aagatccaat tatgtggcgc ggaccactga ttggtagtct agttacacaa ttttacacaa
     5941 ctgttaattg gggtgaatta gactttttac tcattgattt cccaccagga acaagtgacg
     6001 tcgcacttag tgcttttcaa actattccag ttgacgggat tgtgcttgtg acaacaccac
     6061 aacaaatggt ccaacaaatt gtgaagaaaa catataagat gggggaagaa atgaaagtca
     6121 aacttctcgg tgttatcgaa aatatggcat atgttaaatt accaaatagt gatgaaaagt
     6181 attatttata tggtcaaaat aaattagaag acttacaaaa cacattcccg attaatattt
     6241 taggatcact tcctttctta ccacaaaata caaaacttgt tgatgaaggt aaaatagagg
     6301 taacagatac gactgaattc agtgcaattg tcgaaaaatt aataaaggag ttgcaaaatg
     6361 aataaatata gtaatagacg ccaaaaatta tataaagaac ttgcaaataa tagtgcgctc
     6421 attcttttta gtggttgtga gattaagaaa agcgccgatg ggttttatga tttcgatgta
     6481 aataagaact tcttttactt aactggtatt aaacaagaag gttcaatttt agtcgtaacg
     6541 aaaattaaaa acatcgtgaa agaaacaatt ttaacgagtc catatgatgc ccataaagaa
     6601 atatggacag gtcgccggtt aaaaccagaa aatgtaagag aaataactgg gattaacgaa
     6661 tataaaacaa cagatgaatt taatgcattg ctacaaacat taaaaggtaa tgtaaaaaca
     6721 gtttatcttg atttagaacc tggcattttc acgcatactg gtttattagt aagcaatttc
     6781 agagaagaaa tcattaatac cgttaatgta aaagttgaaa atgtctaccc gttaatcgtt
     6841 cgtttgcgca tggtaaaaga tgaagaggaa attgaactta ttcgcgaaag catcgcttta
     6901 actaaaaagg cacttgattt tgccataagt aaagtaaagc ctggcgctta tgaatatgaa
     6961 gttgccgctg cttttgaata taaaattaaa gcggcgcatg gtgttttggg ctttagtact
     7021 atcgccgcta caggtgataa cgcggtaatt cttcattacc ctgacttaaa cactaaactt
     7081 gaagcagggg aattattact ccttgattta ggtggtcata ataatatgta caccgccgat
     7141 atatcgcgga ctgtgcccgt taacggtaaa tttactccgt tacaaatcac catttacgaa
     7201 atcgttttag catgtaataa agcagtgatt gattttatta aacctggtaa aactatcgct
     7261 gacttacaaa actatacacg cgaattttta aaagagcgct tagtgaaagc aaaattaatg
     7321 gataaagacg aagatatcag taactactac tatcacggtg ttagtcatca tttaggactc
     7381 gatacgcatg atcctgctga tcgcgcttta ccacttgaaa agggtaatgt aattaccgtt
     7441 gaaccaggac tttacttcgc aaagtacggt attggtgtcc gcattgagga taatgtatta
     7501 gtgacagaaa aagggtcaga aaatttatcg attagtattc caaaagaaat aaaggagatt
     7561 attagttaat ggcacaatat attttaacta ttgaccaagg aacaacatca acgagagcga
     7621 tgctctttga taagaatgcg gaaattgctt ttatcgccca agaagaaatt aagtgctact
     7681 atccaaaacc aggatatgtt gaacaagatg cgattgaatt atggcttagt gtccttagtg
     7741 ttattaacca agtcttaatt aaagcgaatt taacatttca taacattacg gcgatgggaa
     7801 ttacaaatca aagggaaacg acgattgtat gggacaaaaa cactggaatg cctgtagcgc
     7861 cagccattgt gtggcaaagt cggcaaagcg ctgatatatg taatgcttta agtaaacatc
     7921 aaaatatgat tcataaaaaa acaggcttac ttattaaccc ttattttagc gctagtaaga
     7981 tccgttatat tcttgataat ttaccaaacg gacaagaacg cgctgatgct ggtgaactta
     8041 tgtttggtac tgttgactca tggttaatat acaaattaac gaatgaaaca cggcatgtta
     8101 ctgatattac taatgcctca cggacgatgt tattcaatat taatacttta gaatgggatg
     8161 atgacatctt aaagttattt aatattccgc gtagtatgtt acctgaagtc ttagctagtg
     8221 ctgactattt cggtgaaaca agttattttc ataatacaat cccgattact ggcgtagcgg
     8281 gtgaccaaca agcagcatta tttgggcaaa tgtgtttaaa gccaggtgat gccaaaaata
     8341 catatggaac agggtgcttt atgctgatga atattgggga taaagtgcaa ctttctaaaa
     8401 acggattact cacaacagtt gcgtggaaga taaatgggaa agtgacttat gcactagaag
     8461 gtagtgtctt tatgggtggc gctagtgtgc agtggctacg tgatcaacta aaacttttta
     8521 aaaatgccgc tgatactgaa aatatggctt acaaagcaca gcaaaatgat agcatttact
     8581 ttgttccagc ttttgttggg ttagggactc catattggga cgataatgct cgtggtgccg
     8641 tttttggctt aacgcgcgaa gcaggacgtc acgaattagc gcgcgcaaca cttgaagcga
     8701 ttgcctatca gtctcgtgat gtgtttggtg tgatgattaa agaatcaaaa caagacttaa
     8761 aagttttaag tgtcgatggt ggtgcgtctg ttaataatta cttaatgcaa ttccaggctg
     8821 atatattaaa cacaacaatt cgtgttccaa agatttacga aacaacttca cttggtgttt
     8881 gctatctagc gggtctacac accggcttct tcagttcaat cgaagcaatt acaaagattc
     8941 atgagtacac acgaacttat gaaccaaaaa tggaggagaa agttcgggaa cgaaaatatg
     9001 ctggatggaa aaaggctgta cgcgctgtat taaccttcag cgaagaagag taacattaaa
     9061 ctattaatta cgctggttaa taagccagcg ttttttattt cttctaagtt atattaacaa
     9121 aagttaataa aactgtagaa aaagtattgg gggtcttaac ttactttgct ataatcaaat
     9181 atatgaatga tttggttaag cgtttaaaag gctatgacta tgttattgtt gattatgcga
     9241 caaaacttga acttttaaag ctgcgtataa gaaatccact tttaactttt aaaatattta
     9301 cagttgaaga attaattacg aaatttttag ggagtgctag tgatctaagt cttttcgccc
     9361 tacttgaaaa atataataat ctaacttatg aacacgcaaa gataattaat aacacgattg
     9421 tttttcctaa tgtaagtaaa agtagtttaa aagttaatac attatcaaca tatcaaaaag
     9481 ccttgaaagc tttaaattta ttagaaagtg acttcttaat cgctcatagt ttacataata
     9541 agaaaatttt attaccaaaa tatttagcaa gtaactactt actaattaat ttgttaaaca
     9601 gcgttcacgc taattttgaa tttactgagg acatgcataa ttttattcat gaaatcacag
     9661 tttttcatac cggtgttgat gaagtttacg ctgctttaaa taggattgca gctttactag
     9721 caaatggagt gaagccttcg tttattaaga ttttgaaacc aaatagtgca tatgaagaat
     9781 tattacgaaa attagcgcca cagtttaata ttgtgcttgg accccggcta tttccactta
     9841 atgccttttc tgatgtagtt gcattcgttg aacaatttaa aggacagcga atagatatta
     9901 gtgacccact tattcaaaca ctgcggatga gtacaagtgc accattaatt gctctctaca
     9961 atttaatcat tgctattgat tttaatagtg ttagtaataa gcatcattac gctattctaa
    10021 aagataagat taaaagtgag tcaattgctt tagaggacaa tgacgggatt gaatttatta
    10081 atgaaatacc gttatttagc gctaacgaca cctattattt ctatcttaat ttcgctaatg
    10141 gtgctgcgcc aaatattcaa agcttaaatc catatttaaa taatgaagaa cgcaaacaat
    10201 taggtttatt agatgacgaa acaattgctt taattaatga aaataaatta ataagtactc
    10261 taaacaataa taagaatatt tatatatcat ttgctaagat atttggtcaa gaaaattatg
    10321 aattaagtac attagccgat aaactagaat atgagtgcat tgaacaccaa ttagcgcata
    10381 aaatttatag tcaaaagtat tttaattatt taacgggtgt ttcttacgat aaattaatct
    10441 tttataaaaa acatgacgaa aactttgatg aattatttat cgctaatgat tattttgatg
    10501 attatggaag ttttgattat aagtttaaag ggattaatca ttaccatgaa atggtatatt
    10561 tatcacccac taaaatcgat ttatatcaac gattaccatt tgattatttt gtacaacatt
    10621 tacttaatat cgtagatgac cggccaagca ttcatcttca ttttggagaa tttgtccata
    10681 gtgtattaga aaatagtacg agtgaagaaa catttaaagt atgttttagt cactatcttg
    10741 accaaatgaa ttttaatact aaagatcgtt ttttcgtaga aaatgagcga gatctaattg
    10801 ccgaagcttt tagatttaat caacggtatt taaatacagt tatgcccact aaaattcata
    10861 atgaaacact aatttatgcc ccttttgctg caaaatgtgt attaaatggt cgattagatc
    10921 gtattttatt atttgaaaaa gatgaaaaga aatatatatt tgttgttgac tttaaaactg
    10981 ggaatgcaac cactaatcct aaaaattatg aatatggttt agacttacaa ttaccgcttt
    11041 acggactact tctttcttta actgaagatt acaaggatta tgaagttagt gctcttgtat
    11101 taaactccat taaacttact agtacatata gtgcgggtag tcgagaaaca tttacagaag
    11161 caattattaa tgacatcaaa tacactggta ttttaattga tgacgctgat agcctaaaaa
    11221 tattaaacgc tgaagcagat agtgagcgac cattttttaa aataggtgga cgcgggaaag
    11281 ttaaaaaact tacggactta atccgtagag aagaatataa aaattatatg gaagtgacgc
    11341 gtaataaagt cgaagaaact tatgtgggta tattaaataa tgattttaga attgaagata
    11401 aaaagataaa taacgaaagt agcgcgaaat attcacgcta taaagaaatt agtttcttac
    11461 cgaggaacag tgttattgaa gaggcagatt atgaagaagg aaacgaataa gaacacaata
    11521 acatttagtc cgaaacaaga ggaagctctt agtttaaaca aagaaaatat tcttgtttct
    11581 gctggtgctg gtagtggtaa aactgctgtt ttagtcgaac gtattattcg tattttaatt
    11641 gataaagagg ttcctttaga acggattctt gttcttacct ttactagacc tggagcgcaa
    11701 gagtttaaaa atcggataaa aaaatccttg cttgaaaata aaacgcatgc acataaagct
    11761 tttgcggtcg ataatgcaga tattacaaca tttgatgctt actccttaaa aatattacgt
    11821 aaatatggtt atctccaccc cacaatgcaa agtgatataa ctaatttaga cgctaatttt
    11881 gaaatggtgt taatgaaaca atattttgat gaaattatgg ctgaatatta tgaaaatccc
    11941 ccgctttcat tacgctattt tgcgactagg tttcttagta aggatgatcg acaattattt
    12001 gaactaatta aaagtttaga ccaacaagtt aatttacaaa tagatccaga agcttacctt
    12061 gataattatt tagactcctt ctattcagat actaaatttg aagaaattta taaagaacta
    12121 gatgcttatg ttgtaactac gatacgtaaa gtcgctaatg ccattaagca ttttgaatgt
    12181 gaaagacatt tagaagctta tgaaacattt ctagaccatt atggtgcgat attatcgttt
    12241 aaagattata tggataacct cgataaattt acttatcagc gacggccaag tggctcatta
    12301 aaagagcatc ctgaagattt accgcacgct gaagaagtta agatatacct taatgactta
    12361 aaggaagtac aagtttttga atcaaaagat agaatgtatg atgtctttag tgaaaataag
    12421 ggtcatgcag cgtttgttat tgaaatatat tcgcaaatta gagcaaaact tgcagcgtat
    12481 aaaaaggaaa aacaagctta tacttttagt gatatcgctc gcggtgcttt tgagttagtg
    12541 agtattcctg aaattcaaca tgaacttaaa aatcaatatg attatatttt gattgatgaa
    12601 tatcaagata caagcgatat tcaagaacaa tttataaata aaattgccaa taataatgtt
    12661 tatatggttg gtgatataaa acagagtatt tatggtttta gaaacgctaa ttgtgatata
    12721 ttccgtgaaa aatatgctag atatcgcaaa aacgaaggcg gtaaaaagat tgatttaaat
    12781 gataattatc gaagtcgtag agaagtctta caatcaatta acgctatttt aagtgaaata
    12841 atgactgaag aatatggggg cgctaactat aaaaaggaac atgtgataaa ttcgggcaat
    12901 cttaaatatg atgaattcaa agaagaaaga caaaaagtag gattagaagt aattaattat
    12961 gttgtgccta aaaaaaataa gaaaaatcgt gataataatg ttttaatccg tgacaagata
    13021 gattatgaac ttgattacat cattaatgac atcgaacata aaatagttaa taaatatcag
    13081 gtttatgaaa ataaagtcgg tatgcgtgat gtgcgttatg gtgactttac aattttgatt
    13141 gcgcaaggaa cgaaatttaa tcgaatagaa gaacgcttta tccgcgcagg aattcctgtt
    13201 tatgttgatc gtgatgaaga agcaaatgaa aataatatta atatcgcgat aaataaatta
    13261 ctaactattt atgttgctta cacaaaaagt gatgagccga aaggttttga attccgttta
    13321 gcggtcgcta gtttcttacg cggatttgtt aataaatata gcgaccaaca actttttgat
    13381 ttattaaaag atgataattg ggattttagt ggtgacccaa tactagcacg cattaaaaag
    13441 ctagcacaaa ccactaaact cttacccgta acagaaatta ttgagcgcct cattactgaa
    13501 tttgatgtat atggtgcact atacaatatt cgagatttaa cgcaaaatac tatgaaattg
    13561 tcgagtaaat tagaagaaat agaaaattta gcggcattag gtttagattt agaagctatt
    13621 gtaaattact ttaaagaacg tgagattgcg ggagtcaatg ctgccttaaa acgtgtaaaa
    13681 ttagagcatg atgctgtaac gattatgaca atccaccgtt caaagggtct agaatttccg
    13741 attgtctatt atatggacct tgaatctaat ttttatcaag taagtaggag tcaagtgatg
    13801 aaggtatgtc caaagtacgg ttttatttta cccgaaccgt cctataaatc ccaaaagact
    13861 ttcattggtt tcttgtcaaa tatatataca tctaaagaaa cattatccga gaaaattaga
    13921 ttactttatg ttgccctaac acgtgccaaa gagttgatgg ttgtcattca ttctactaat
    13981 gaagatggag aaccttataa aacttttgaa aagtctagta accttaagca actccttctt
    14041 caatcaactt actatgatca aaagcgttta gtagttgagc ctgaatacac acaacagcga
    14101 cttagcgaga gtgaacatag taaagaagtt aaatacattt attcgcttca tgaacatttt
    14161 tataaattta ataaaaagcc taaaacttac acggttagtg aaatagttga tgttgatgag
    14221 aggcttttag cgcgtggtac tttgctccat cgttattttg aagaatatga ttttactacg
    14281 aaagatctta attatattag tgatgaaaga gatcgacaac atatgcttaa cctgttaagt
    14341 ttacctttct tcgctaatgc taatgcgaat aatatattcc gtgaatatga atttattgat
    14401 gatggaacaa ataaaaccta tgcgattgac tgctttatca ttcaagatga taagataatg
    14461 ctttttgatt ttaaactttc aaatatttat catccaagtt atgatgttca agtaagaaac
    14521 tatgcacatt atttacaaaa ggtttttaag aaaagtgttg aaggctattt aattagtatt
    14581 actgacttaa aagtgaggaa agtatcaaat gaatgataaa aaaacatata caccgataag
    14641 taatgaaaac tttttgcggt tgcttcggtt ttataaaatt ccagaatcag cggaagatga
    14701 agtcttatac aatttataca tagaaaccgt tgaacttctc acattacatc atcagacttt
    14761 tgaaaatata ccgtatatta atcttgatca tcagcgatta atattacaac ttattcatga
    14821 ttatgatttt cggatgcgtg gtcttaactt tgaagaacgt cggtcactac ttaaagatga
    14881 actttttcat aacaaactca ttaatgttgt tgttgataaa tatgggagca gcgctatttt
    14941 taagtatgat tctggcactt atttaactcc gttttctatg gaaatatcga caattaatgt
    15001 ttatttaaat ttcataatgc ttaaattagg atcgattcca cgccataata aagcgacaga
    15061 actatatgcg gaattactga caagtgcttt ttcctatgtc cttacgatta cagaattact
    15121 cgtgcgcggt tttgaaaaag aagcgcttgc tacatggcgc tcactgcatg aattagaagc
    15181 aacactactc ttaattcaag atgaaaaggt actcgctcaa tataatcaac atattcttta
    15241 cgccttagcg tttaataaac ttatcgcaca agcagaaagc gataaagttt ttattgaaat
    15301 taaagcgaag atgaaagact taaaacttaa aagtaaagat acgaaaagat ttattgaata
    15361 cggctggctt ttagcgcaca acgattttga tttaaacatt cataagttta attttcgtga
    15421 cggggtgcag acgctcgctg gactaaatca taaacgcgat atttatcaaa ttgctagtga
    15481 agttacacat agttcgccac ttacattatt tactaaacga cattactttt taagtattgc
    15541 ccttgaaaat ctatacagct catttttaac aattgaagtg ctttttgcag cgttttacat
    15601 caaaaacacc acaaaaaatg aagcggaatt ttatgaagtt acgcgttcta tttatctaga
    15661 agatattaac tttgtaaaag atcgcataac aaaataaaaa agagccactc ggttaaaagt
    15721 ggctcaagag ttagcaatta ttaatgatta tacatcttca aaacagctgc cgccgataaa
    15781 ttcacggagt aatgaattag aaggtaccca cttttcatcc atatcaagga aatatttaag
    15841 catccggatt aagcgttccg caataggaaa gaattcactt aaataatcaa attcaagcaa
    15901 gagcggtaac gcatgctttt tcataaccgc taattcgtaa gagagcaatg aattatatac
    15961 gtaatcggct tcttcttgtg tgtcataaat ccatttaaat tcaccagcgc gaacacttgg
    16021 ccacattgcc attgtttctt tagcactagc gttacgatat ttcttatcgc gaacaatacg
    16081 acgtaataat cttaaatcag taagactaat tgggttatgt tcatcaatgt taatttgtgc
    16141 ttgtggtgca atataaattt tatatttttg gtgttttgga atataactag ttaattgctc
    16201 attaagtgcg tgaattcctt cgatgatgat tggttgatta gccgctactt ttagttttcg
    16261 tcctggtacg cgttgaccct ttttaaagtc aaactttggt aattcaactt cttcaccatt
    16321 aattaagtcg agcatgtttt tattaaataa ttcgatatca agggcataaa ttgattctaa
    16381 atcatagttg ccatattcat cacgcggtgc taattcgcgt ggtaagtagt agtcgtcaat
    16441 tgataaacga atagcgttaa gtccgcgact cattagttca atgcgtaatc tgtttgcaaa
    16501 tgttgtctta ccagagctac ttggacctgc aatacaaatt aagcgaatat cttcggaatc
    16561 ttctaaaata cgttgtccta attcacttaa catttggtaa tgtttagctt cggcgatatt
    16621 tattaattcg acgatatggc cattttcaat atagtggtta atatgggcga cactatctaa
    16681 atttgttaat ttagcccagt tgtgactagc ttttaatgct ttgccaaaca ttggtgcatc
    16741 tttaaattca ggaatttcac cttttgtttc gctacgggga tattgaagta gcattccagg
    16801 tgaataaggc ctaattataa agttttttaa ataacctgaa gaaggaacca tatatccata
    16861 catatagttt tcatagccat cacattcata tatatgcaca gtcggttctg gacgatattt
    16921 caatatttcg agtttatctt tatatccaag ttcttcatat aacttttctg cttcttctat
    16981 tgttatttta gtgcgattaa atggtaagtc cgcttcaaca atattacgca tttcccgttg
    17041 caatttttca acaagcaatc gatcaacagt gacgccttta tttgttattt gaatgaagat
    17101 tgagcgcgac acattatatg aaaaacgcac atttagttcg ggtctaattc ttttcattgc
    17161 catcgctgct acaaagcgaa gcgatgtttg ataaatgcga ataccttcgc cattatctag
    17221 tgtctttgtt tcaagtatcg catctcgatt caaaagaaat gttaattctc ggacgcgtcc
    17281 attgacataa gcacaaatta agttcttatc cggacctaca atatctaaga tacgtactgg
    17341 ctcgtcatga gttgtagttt ttccattgta aactacagta aaagtttttg tttttttatt
    17401 ttccatagat tctcctttta tataaagctt aaatagtaag ttaattatat acaacaacaa
    17461 gtttttttcc taaagaaata acaatagcaa ttattaatta gctaaagtac gattcgtaat
    17521 tagatatgat cgtttcaagt tcggtgtcgt cattaggtaa tgatatttcc ttattactac
    17581 tcccgtaact caaagtaggt gtatctaaag gggtgtcctc atttactaag taatttacat
    17641 aacctttctt ttcaaagtaa ggaaaaaact ttgtattaat taattcgtga tgagtagcgt
    17701 tattaaaatt atatgtaaat gtcacatact ttttaaaatc aggtttaatg ttgtcttttt
    17761 tatctgttgt gtaatagtta tttgtgcgaa catgttcttt aaaaaattta ttaacttcca
    17821 attgtgtact taaattacta gcgccacgtt cgcgatggac catctcacct tctttatata
    17881 tcaaaatatt cggtgcaccc atcacattag ttgtttcaat ataatattcg cgattatcgc
    17941 catattcttg actataaagg tcaatataat aaatttcgat tttggttttt tgcacgtaat
    18001 ttagtaataa tggttcaatc tctgcacaat attgacagcg atgactacca aaatatagca
    18061 cgaaagattt tttccctttt gtaaaagctt catgatatgc ttttgtcata ttatatgtat
    18121 aattttcact tgttatagta gtttctaatc gttttggtac gagtatttga ttagggttag
    18181 aaactttaca tccgcttaaa aatgagctaa aaatgtaaat aaatagtaaa agttgtaata
    18241 tcttttcttt tttcatatta taattaaacc acaatcataa ttagaaatca tttttcaaga
    18301 ttattacatt taaggagata ttatgaatat ttggcatgac atggacccac gacgtattac
    18361 aactgaacga ttcgatgcag ttgtagaaat tagcaggggt caacaatcaa agtacgaact
    18421 tgataaagaa acaggattaa ttagacttga tcgcgttctt tacacctcaa cccactatcc
    18481 acaaaactat ggatttattc cgcggactta tgcaggagac gacgacccat tggatgtgct
    18541 cattttatgt tcacaacctt tagtaccgat gacaattgtc acatgtaccc ctattggtgc
    18601 ggttagaatg cttgatggtg gtgaacttga cgaaaaaatt attgccattg cggcaaatga
    18661 tccaacttat tcttcgtatg catcggttga tgaaatcccg caacatgtgt tacaagaaat
    18721 tcgtcacttt ttccaagtgt ataaaacact tgaaggtaaa gaaacagtcg tacatcatat
    18781 tcaaggccgt gataaagcta ttgatataat agagaaatct attgaaatgt ataacgaagt
    18841 atttaatata aaacaagcgg aagaataatg ctaaatatta ttctttatca accagagatg
    18901 ccggctaatg tcggcaatat aatgcgtacg gcagtggcaa ttgatgctac gctacatatt
    18961 atagggccga ccccaattaa gcttgatgat aagagtttaa aacgtgcagg gatggactat
    19021 gtccaagact tatccttttt tatttatgac tcaatagaca aatttatgga aaaacatcac
    19081 gacaaaaata tttttttcgt gacgcggtat agtaaaaata attatacaga agttgattat
    19141 ggtcgttttg atgctggtat atatgtgatg tttggtaatg aatcatatgg tttaccaatg
    19201 gaattattac gcaagcataa agaaaacacc ttgcgaatac caatggtagc gactgcacgt
    19261 agtttaaatc ttagtaatgc tgtggcagtt gttagttatg aaatattacg ccaacaagga
    19321 ttctataacc ttgccacaag tgaagtaatt aaaggtgatg attatttata aaagcgcttg
    19381 atattgtttt caaaaaacgg ttttaaatca tcattaaagc gtaaaaagaa tgtattatat
    19441 ttacgggtta gtacttgtcc agggaacgcc ccctttttaa tattataaat cggtgaatac
    19501 ataacttcac catcaatttg ttttgctaaa aataagcata acattccagc aaactctaac
    19561 acatcttttg ctggattttc ttgcctaata attatatgcg ctccaggccg attttgaata
    19621 tgcataaaag tatcattttt cttagcaatt ttaaaggtta aaaagtcgtt ttgtttatta
    19681 ttttttccaa ataaaatatc aatatcttta taagttatta catacggatt taatttagaa
    19741 attatttgcc gtttatcacg tctttttaat agtccaagtt cttcataaat ttggaatatt
    19801 tctccagcac tctctagact ttttaaatga ttttcaatac tttccagata ctttatcttc
    19861 tctttagttt cagcaatttt tgacatcaat atattttcgg attttttaat ttttttagct
    19921 tttttgaaaa gaatctcagc gttaaaaagt gcatcaaaac gcggatcaac ttctatagtt
    19981 tcaccttcaa tttgcacaaa atagtacgaa attgctggtt tatatgttaa taacatattt
    20041 gctttgtggt ataaatccgg aagttctttt aacttttcta agtcttgctc ataattcctt
    20101 attagtcgtt ttgagcgttt gatatttgtt tctaaatata cataaacatc acgaaagttt
    20161 tgctttttaa ttcgttcacg attctcttta agatatgcct gtaaataatc tgttttagcg
    20221 ttagaaattt gcggcgaaaa agcacctttt tcaggtagtg aatatttact gccaccacga
    20281 ataaatcgac catcttttgc gcgttcttga tgatagcgat acgctgctaa aatacgtcct
    20341 ttattattca ctaatacagc ttggggatga cgcggaatta attcaataaa aagtgtgact
    20401 tcttcaagtt ccaaaacatt gtttctttgc tcgaaaacta gtgataaaat catatcatca
    20461 ttaggttgat tcatagccac taagttacca cctaaaaact gtcttaaatt tatggcaaaa
    20521 ctatgtaatt ctccgccctc ggggaaatca cttaaatcag cgttaaacac aataaaaggc
    20581 gtcggactat ttaatgagac aaataagtta ttaccatgac taaattcaaa tacaaaatca
    20641 accaaactta attgacgaat tcgcactaga aataaacctt caaaatgttt attagcgtta
    20701 attaattgtt gtaaatcatt tggaaaaagt ctttgcatgc tcttattcta caacaagaat
    20761 gtgacaagtt tgttatttat taataaataa gatacaattt attaaggaaa atcaagggga
    20821 aaatatatga gacaaggctt tattgtttta ataagtggtc catcaggtgt aggcaaagga
    20881 acacttattc atagtttact tgccgatcaa acattgaact taagttttag cgtttcaatg
    20941 actacaagaa aaccacgtcc taatgaaaaa gatgggattc attatttttt tgttagtcat
    21001 gaaacatttt tgaaaaacat cgaggaaaac aattttttag agcatgctca gtttgttgga
    21061 aatcgctatg gaacgcctcg cgactatgtt gaaaaattat tgaatgaagg caaaaatgtt
    21121 ttaattgaaa ttgaagttga tggtgcccgt cagttattaa aaaatgttga tagtgaaaaa
    21181 gttctttccc tttatataat gcccccttca gttcaagaat tagtaagacg cttacgctta
    21241 cgtggctcgg aagacgaagc aacaatacag cgtcgcgctg aaagatttaa agaagaaatt
    21301 aaatataaag accaatatca gcacattatc ataaatgatg agttagaaac gtgtattaat
    21361 gatgttaagg cattaattcg ggagaagatg aatatttaaa tatgcaactc attgaagttt
    21421 ttacacaata ttcaaaatta aatcgtcctt ttacatatta ttatgatggc gatgatttag
    21481 aagttggagt aagagttctc atcgatttta ataatcgtga aattgttggt tatgtagcgg
    21541 ccgtcaaaag caccaaagag acaattgaaa cggcaagtaa acgcctaggc tttgagcttg
    21601 ctgaaataac aaagattctc gatgaaaaac cactcttaac taacgaatta atggaattga
    21661 gtgaaaatgt tactagttat tacttaacaa atcggattag tgttttaaat gcgatgttac
    21721 caccctcact acggcctagt aaaggtagtt taagtgcgcc aaagattgca atgcaaaaaa
    21781 aagtaagttt taatcctgaa tttactgatt ttgataagtt aacagataaa caaagttcat
    21841 tattaagtga aataatgacg catggtagtg ttttacaaac ttcaaaaaat agaacgcaaa
    21901 taatgcagct attaaataaa ggtgctgtaa ttgtggaaga gattgaaaaa tacagattac
    21961 gctacgaaga ttatccgcgt gaagatccac atgaattaac caatttgcaa aaagaagcag
    22021 taaaatccat tttagaagat tataaagaga catttttgct ccatggtatt accggaagtg
    22081 gtaaaacaga aatatatctt gcccttgccg ctcactattt agcacaaaat aaacaagtta
    22141 taatgctcgt accagaaatt gctttaactg gaacaatgct tgctaatttt tatcgaagat
    22201 ttggtaagaa aattgctatc cttcatagtg aacttacacc agctgaaaaa tacgatgaat
    22261 accgtcgcat cagcgctggt gaagttgata ttgttgttgg tgccaggagt gctatttttg
    22321 ccccattaca aaatattggg cttattatat tagatgaaga gcatgctgaa acttataagc
    22381 aagatgttgc acctttttac cacgccttaa ctgtagcgca aatgcgggcg aaaagcttta
    22441 acgctaaagt attagttggt agtgcaacac caagcattga aacgatggcg cgcgctagaa
    22501 aagaagttta tggttattat tacctaggtg aaagaattaa cttgcagccc ttaccagaaa
    22561 cgacagttgt taatatgtta gactataaaa atgttaccac tacttcacca tatatttcta
    22621 agttattagc gaccgaaatt agaaaagcta ttgatgccaa gacacaagtg attttattag
    22681 taaaccggcg ggggtacgcc acttttgtaa gttgtcgtga atgtggcgaa tctaaaaagt
    22741 gccctgcctg tgcgatatca ctcacctatc ataaagtagg aaataaccta aaatgccatc
    22801 actgtggcct ttctttccct aaaaatgatc cttgcatcaa atgtggtagc actaatttct
    22861 catatactgg atttgccaca caaaaagctg aagaagatat tcaaaaaatg tttcctgatg
    22921 cacggatctt gcggcttgat agtgatgtga gtcgtaaaga taaaacacca caagtagtgt
    22981 taaacaaatt tatgaaccac gaagcagata ttttaattgg tacgcaaatg gtttcgaaag
    23041 gtcatgattt cccattagtt agtttagtgg gagtaattgg cactgatgct aatttatcaa
    23101 taccctcatt tagaagtagt gaacgaacat ttcagctaat cacccaagcg attggtagat
    23161 caggtcgcga aaaaataaaa gggaaggcga ttattcaaac aatgatgcca gaacattatg
    23221 ttattcaaca tgcaaaacaa caagattatt ttggatttta tgaagttgaa attagacatc
    23281 gttatttaac aagtaatccg cctttttatt ttttattatc aattacgatg agccatcaaa
    23341 acgaaagaac ggtaaaagat actatattaa cattaaaact aaatcttatt aaagcactgg
    23401 gtgaagatgc gactgtgatt ggtccaaccg ctacttttta cccagcaagt ggtaaaacct
    23461 ttaacgaaac acttattgtg cggtataaaa attattttaa aattaaacct gcacttttaa
    23521 ctacattaag tccatttaga gaacaatctt ctctttatgt acatataaat gttgatccat
    23581 atgatgtata gaaaggaaaa atattatggt cattattaaa atttattctc aagacattta
    23641 tgtcgctgct gaaatcagtg ttaaatacgc tgatgctatt gctaaaacac taggtttaac
    23701 aaaagaagat gtattcttac tcggtagtga tggtgcttta atcgcggaag gtgttgatca
    23761 agtaagttgg tttacatatt tagaagttac tattcctgca aatttaaagc acaaacaaga
    23821 tgcattaagc aaaacgcttt tagaaatatt caataattat ggtgtccacc ttatgattaa
    23881 atatgactat gtaacaaaag ataatattgt taaaatttta aaccctgatt ttaaagtttt
    23941 taatgaaaca atggttgaag ttttgcctac tggcaattac tacgatgaag atgatgtcga
    24001 ctgggatgat gattgggacg aagacgaaga caaagaggtt agataacata cttatcttta
    24061 caaataaaga ttaaattttt aaaaacaaac
//
LOCUS       DAOSTA010000015        24045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_2276_length_24045_cov_8.892414, whole genome shotgun sequence.
ACCESSION   DAOSTA010000015 DAOSTA010000000
VERSION     DAOSTA010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 24045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24045
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2276_length_24045_cov_8.892414"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..858)
                     /locus_tag="PLU08_02480"
                     /pseudo
     CDS             complement(1..858)
                     /locus_tag="PLU08_02480"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
     gene            complement(1126..2103)
                     /locus_tag="PLU08_02485"
     CDS             complement(1126..2103)
                     /locus_tag="PLU08_02485"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOQ70602.1"
                     /translation="MDWVEKLKHKYNVDVLYDVFLRENDSLFFLIEESHDICDKPLIK
                     LFGTCEGVGLPYCVDTIDELLSHIDVKSQICDIRGANEYRDELHREINSDQTKFVLYL
                     PLDFKGTKRYLQLEIIKMFKRNISIVMLNQIDIKKVNVETLFFELYKDSLTNLFNFST
                     LLMHIDKTEDSHYFGFLDLDYFKVVNDTYGHKVGDEILAKVGWKLIEIADETVIMYRK
                     SGDEFIFMTLGLDLNETKKLVKRIQKAIRSVELPDIKIDCSIGVAEYRHNNGFYDAED
                     AVNIADVAMYVSKSTGRGRVTYFNEEQARKVMASGPLEKTLELLESRAR"
     gene            complement(2540..2752)
                     /locus_tag="PLU08_02490"
     CDS             complement(2540..2752)
                     /locus_tag="PLU08_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70603.1"
                     /translation="MVDTNEIRRIFNELERQAKTLLNAANSFENALRDVDNSVDTRNF
                     LEEYKTFYGILDLKTSGLQLVRWKGV"
     gene            complement(2946..5309)
                     /locus_tag="PLU08_02495"
     CDS             complement(2946..5309)
                     /locus_tag="PLU08_02495"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOQ70604.1"
                     /translation="MIYTLKNYQRDAVDELKGYIELGFRSKNRKEVVFKAPTGSGKTF
                     MAASLFEELAGEHSDINFCILWACPGKGELHKQSHDAVKTYLGGNPVCSLLEEDFFGS
                     RKFIKNKEIVFINWEKLIQKDKGTGAWANNLMKDQEGMNFIDVIDQTKKNGTKVVLVV
                     DESHIGSSAKARIQEFINTIIIPSILLEMSATPLNDHIDVEVNPEDVIKEGMIKEDVI
                     VNEGIKKEDKSLEEKDSELLILEKGFAKREEIVKKYAELNINVNPLVLIQIPNVDEGE
                     AKKLVIKDFLREKGITEANGKLKLWCDNHDPFDKKEIRKNNDITEYLIFKTAVATGWD
                     CPRAHILVKFREGKSETFETQTIGRILRTAEAKSYDDPLLDNAYIFTNIWTFETKQDS
                     YNPNKIKTEWTKMREGYTQLRVWAQTKLISFYRSRQEDYNSADSRFGEYLFKSFMKRM
                     GLTEEDRNNVLFNDPNRLEDAGLKLETNTGDVILEETNLGMENIAEERTITGNAVNVQ
                     MSENDVQAQYNAIIRDNLNGLAYVRSKSPINTAIMDVLSTFIKGFPRAEKVKRYMQIV
                     VNNRELFAEVISEATAEFRQMLSVNAGKKGVRYDFKIEESRAYSSETHTTLLGIKSLY
                     NPLFVLKNASGQVNYLEKNFLQYLDTQNVVEWYWENGSELMRINFGISYNNGMNTFQP
                     DFIIKFKDGSVGIFDTKPNDETNLADTTVKNEALRDYLYDINQNRGYDPKVVGGIVCQ
                     SGTQFYLFEGRDYHDYNSNHDGWVNFSEFLRKIEDAYDIEKALKKLK"
     gene            complement(5312..7006)
                     /locus_tag="PLU08_02500"
     CDS             complement(5312..7006)
                     /locus_tag="PLU08_02500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOQ70605.1"
                     /translation="MADFDIEQLLKQIKELESELKASKKYGLVWDKENTKEEVVLLCE
                     KYIPTLSPIKSLNIKKGGLSHCLIEGDNFHSLTCLNYVLQDSFDLIYIDPPYNTGSED
                     FLYNDNYINSDDTFLHSKWLNFMQKRLTLARNLLKQDGVIFISIDDNEQANLKILCDQ
                     VFGPQNFVNCVVVKMSEMSGVKMSHVEKKLPKLKEYCLVYSKNRFYYSMNPIKNNRLD
                     NLDDFAKYAKYYSKIIVDKSKPVEEWEILPLDKYLKRIGKSNLTEREILQFKLDNAER
                     MIYRTNNKSFEKIQCDKPVQKIISATGIEYIWWEGKQMLFLSDYTTTFEGDLWTDIST
                     INLNKEGGVDYQNGKKPLKLLSKIISTVKNKDALCLDFFAGSGSFGEAVLMANAEDGG
                     KRRFILCQSAEENDIAKTITYPRLKTVITGIKKDGSKYSDGLSGSLYYYKTDFIKNEA
                     NTEQAKYNLVEKVDALLCLAENIFDEVERNDYSSHFKSGDKRLFIYNDYYNAQKFEEF
                     KSRVLASQGEKIVYVYSSDNNVDNSLIEGMDVVLKPIPSKIYEIYKEIVEDIKRDE"
     gene            7135..7416
                     /locus_tag="PLU08_02505"
     CDS             7135..7416
                     /locus_tag="PLU08_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ70606.1"
                     /translation="MRLRELRNKSGLTQNEIANKLGVSGQTILNWENGIYEPKINQLI
                     ELADLFDVTVDYLIERKTSYKSVDAICKELERIPKENVINFIKEELQKL"
     gene            complement(7411..7629)
                     /locus_tag="PLU08_02510"
     CDS             complement(7411..7629)
                     /locus_tag="PLU08_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70607.1"
                     /translation="MEGINHEYVTRVHNYIVAMVYFEGLLDKGEITIEEYIEIERRIA
                     FKYKLEESIFRMEKRACDYGSTIEKIEL"
     gene            complement(7847..7923)
                     /locus_tag="PLU08_02515"
     tRNA            complement(7847..7923)
                     /locus_tag="PLU08_02515"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7887..7889),aa:Glu,seq:ctc)
     gene            complement(8015..8091)
                     /locus_tag="PLU08_02520"
     tRNA            complement(8015..8091)
                     /locus_tag="PLU08_02520"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8055..8057),aa:Glu,seq:ttc)
     gene            complement(8095..8169)
                     /locus_tag="PLU08_02525"
     tRNA            complement(8095..8169)
                     /locus_tag="PLU08_02525"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8135..8137),aa:Asn,seq:gtt)
     gene            complement(8236..8460)
                     /locus_tag="PLU08_02530"
     CDS             complement(8236..8460)
                     /locus_tag="PLU08_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOQ70608.1"
                     /translation="MTNLERIEQIFTELLKVEKIEPEMELKALGLDSLDLVEVMMRLE
                     EEFGIEFSNDEMLGFSTVADVVAEIERKTK"
     gene            complement(8562..8699)
                     /locus_tag="PLU08_02535"
     CDS             complement(8562..8699)
                     /locus_tag="PLU08_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70609.1"
                     /translation="MSELKGQILGLLLVVVIFATIAAAFKASVNNQLSEIASHESALV
                     I"
     gene            complement(8716..9054)
                     /locus_tag="PLU08_02540"
     CDS             complement(8716..9054)
                     /locus_tag="PLU08_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70610.1"
                     /translation="MSNIMSLVYSFILIFPTILLGVDFFIIGKIIVELETRATIVSYI
                     ISEEGGIRPSLVNELAEEGITITCEGECSYISVGETIKYQLIKTYTPIIIKNEDINIY
                     VTRTTIVGYL"
     gene            complement(9051..9656)
                     /locus_tag="PLU08_02545"
     CDS             complement(9051..9656)
                     /locus_tag="PLU08_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70611.1"
                     /translation="MLVKWLERSAGLLLIIVGAILYFWKKNLTLALIFAGVGFTIVVI
                     TFIPQKQKSNITLADFINVFTHFRVALDNNLNVYRALNITLTTTSGAMSALLESLINA
                     LENDHSVTPFINFSLPFKHPLVSQIMINVYMLVNHGVDPKRLWQFNYLFETLVKEHND
                     EEIALHESSYERFNISLFIGTGIMIMTLMISIMGMIGEYIV"
     gene            complement(9646..10335)
                     /locus_tag="PLU08_02550"
     CDS             complement(9646..10335)
                     /locus_tag="PLU08_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70612.1"
                     /translation="MIIIYGGAFFLVIVIAIFSALVLGGIKITIINALIALVVAAFLT
                     YRVTNYRKDIEKRRFMFNFMEYFILNFDIQKTVEATLTTIYPLLDVKKSRVYLTMNED
                     GMLLLEKLRLTFAHQYYESFLEMVKLINEHGGEMLKVAEVLLFSISNSETQLIKLTRI
                     DNAYLIKFIFNWFFIMLVAIVFRLALDGFLKFETLPLIYVAGMEVFIAIFLTSIVLVF
                     ENRIRRTRRVS"
     gene            complement(10332..11258)
                     /locus_tag="PLU08_02555"
     CDS             complement(10332..11258)
                     /locus_tag="PLU08_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOQ70613.1"
                     /translation="MEKVIKVIEESFLAPLLSDKNITDISYNGHKLFYFSNESGRKLY
                     GEINHKTVASFLRNVANFANERFSYADTFLDISIGKYRLSAMHYACSRFEFEESLSFA
                     LRINHNFLASNPHYLNNDVVDVLKYVMKENRSIIISGTTGVGKTTLQKYLLMLLPANT
                     RVLVIDNVLELSEVTTLNKDLDLTLWQSLTNKEEEIKSFIQKALRFHPDYLIIAESRG
                     GEMKEILQGAISGHPNIVTLHADSTALAYERVIYLAKHDDRHSLLQAFPFVIHLAKKY
                     KADGSIYRYVEKITEYNVDTDEIEILYHEVIP"
     gene            complement(11261..11626)
                     /locus_tag="PLU08_02560"
     CDS             complement(11261..11626)
                     /locus_tag="PLU08_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70614.1"
                     /translation="MAWSNFAFLFLFTYSTLLAINIRYHHVNEVLSSVTHDHIYHVTT
                     MDLVTYELYFEKPLVRVVVANHFNLNMKRENYEYTITFYTNGVVSEESERSRYFTINL
                     VCPLGFGVTFDKTFAYYVS"
     gene            complement(11628..12572)
                     /locus_tag="PLU08_02565"
     CDS             complement(11628..12572)
                     /locus_tag="PLU08_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70615.1"
                     /translation="MSVFIPLVPESNFQTSALGSYRNNPGDITQSYEVAIPFIASYTT
                     TLTYRYTVYNSANEIVYTQPSTSTRALRNVRVLITYRATAGKFTVGQNRIILYYKTSS
                     EAERRHYSYFYGYQKGVNVNLNNGSAARQLLEAEVCYLYDSTNRSDIFRKMSFSSANL
                     TPIKHFNHDLYFDFSGLFLSIGVLYDNENFYSSAALYCSNPALFPYLANVGGRAMQYL
                     NLTRAYNRLVTSVKTPLFVRADTLQIARSKYSAEYLNTDTYFFPKYHFDELQNTKFTL
                     EIKDFSYSKFNLKYEFTIQIDHPYFGTSGEHEVIINRY"
     gene            12697..13239
                     /locus_tag="PLU08_02570"
     CDS             12697..13239
                     /locus_tag="PLU08_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOQ70616.1"
                     /translation="MIVLMGASASGKTEVAKMLGKLFSIKKVVTHTTREMRVNEVNGR
                     DYHFVTKEEFLRLKAEDYFVETVEYNDNFYGTSKAEIAMNKVLIIEPIGLKSIRALNS
                     EHIVVFFMQASRAIRRKRMINRGDNPKIATERILIDDAKFNPKNIEGIDYTIDSENQS
                     IKEIAIEVYEKYALKLVNFK"
     gene            complement(13232..14137)
                     /locus_tag="PLU08_02575"
     CDS             complement(13232..14137)
                     /locus_tag="PLU08_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ70617.1"
                     /translation="MYVEKFLERIPSLLNKTYVVTGANSGLGFALSTLLVSKGARVIM
                     ANRNPKRSEDAINKIRELYPAASVMVLPFDQSKKDSIEAFVNSLISHNIKPEGFVLNA
                     GIYSPAKDAKTEDGVALTFGINYLGNFIFTQTLANHGLISAETRLIYISSPAYSRKFS
                     SQILARAISGQKITRPKQYAVAKTAQKIFVLGLMTKSPLIPFENKGKIYLYHPGIAVS
                     NIARFKWKFFNKIVHRFMSVFFHPPEKAVLGALVALTTDKDLNGKILVPRGFLEINGI
                     PRIKKIDEKLLSHLPYLITETLKLI"
     gene            complement(14137..15408)
                     /locus_tag="PLU08_02580"
     CDS             complement(14137..15408)
                     /locus_tag="PLU08_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOQ70618.1"
                     /translation="MGNVIRFAIVILLFALSFFFSSSESAFSTINRLRVERDVEDGKK
                     RSKTLLRIITNYENTVQTILLGNNFVNIAVTALVTGMALAYDNAHGATGQYVAIFSVV
                     LFIVLLTIGEIVPKTLAVKLNYQMSYAYANIFMFFYYLFTPLLFPFRLISARRKKKNG
                     NEEVNGDEIYTEDELQIIVDEIEEQGLIDEETSDLVRSALEFTETEAYEIMTPRVDVF
                     SYDIEDDIKELMKEEDIFKYSRIPVYEDTIDNIIGILRTKDLLKLSLSDKKINVKSLI
                     KPPLFVHHTKNVSQILTEFKQTKNHIAIVIDEYGGTEGIITMEDILEEIIGEIWDEAD
                     EVDEPIVKLEDNHYIIDGGLNIEDFFELFELSEDEDNDYDTVAGWCIEMLDRFAKAGD
                     EFTYEKITVKIIEVEGFVVEKVEVIVESEED"
     gene            complement(15401..16417)
                     /locus_tag="PLU08_02585"
     CDS             complement(15401..16417)
                     /locus_tag="PLU08_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOQ70619.1"
                     /translation="MIDDPQTWVLLILYFVASMYFSSSETAFMTANRFKIRAKAEEGS
                     VSAKLAAWVINRLENSIIAVVVFNNLAHLLFTALLTVVLVNQYGNDTGELLATLISVP
                     LMFLFAETIPKVLGRRYSEQYALISAYLLVPFIIISYPVALFFRGIVWVVKKLFKATE
                     DNIFTADDFEQVIETIEEHGIINEDASDIIISSLEFSETVVKDVITPKNAIVAYDISK
                     FNKDSFKDFVLSTHFSRIPLYDGSLDKIVGVILVREYIKHYQKKPNINPRTIMARPYY
                     VNYKITLTKMVEGFKKNNTHIAFVVDDKKKLLGMVTMEDVLEELVGQIAETSGTVKGA
                     SVNG"
     gene            complement(16508..18811)
                     /locus_tag="PLU08_02590"
     CDS             complement(16508..18811)
                     /locus_tag="PLU08_02590"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOQ70620.1"
                     /translation="MKKIFNFIVRFKVIFFGFWIALTIASIFMIPHVKINYDTTKYLP
                     EDTRVKESLRVMEEEFGITGQASVMIEKVETLQEVFVYKDEIISIPTVMEVVWIDSFV
                     ERDTINDIQRIAEENGMSGVFDFANIPGLNAFYKDKSALLQIVFTEEDHSRAVGKAIE
                     QIRSYLEGIKRPYAMRGTAVNAYYTQKLTESEVLRVTVIVVPIILFILLVFTRSWAEP
                     IIFLIAVGVAVLVNMGTNLFFPSVSFLTNSTASLLQLAISMDYSIFLLHAYQKERAEG
                     LDKKDAMVMAMRKSFVSINSSMLTTAAGFLALMFMRYTIGIDLAAVMIKGILLSIIST
                     FTFMPGLILLTDKIVEKTKHKRFFPLLGGLTKWIVKLRYVVPAILLLVIVPSYLAQTN
                     NSFVYGEAAMSVSEGTPAAIEKNRIEAKFGKENLMAILVPRDEADAYIAQEKAMIKQL
                     YREFERANQDFVVQITSLSNLSDIETYIPNYDDLDQTIKNFLDDIFADIMDNLITEEY
                     KAQFLSEHYSRIIITVNTESESPRAEIAVDIVETVVARQPFARDAHIIGVSPSVKEIR
                     NVVETDYVIVDLISIGFVFIILLVSFRSISLPFILVLVIQISIWINMAIPYLTSQPMI
                     FIGYMIVSSVQLGATIDYAILFTGFYTEGRKTMIRRDAIKYALDSGGHSILTSCLILT
                     SAGYSLKFLSSIEGVSSLGELIGRGAALSGILVLVLLPQLLYIFDKVVEKTTWKMRFY
                     KGGTKVGRVIIEDEGYSNSDQDPTLLS"
     gene            complement(18808..19374)
                     /locus_tag="PLU08_02595"
     CDS             complement(18808..19374)
                     /locus_tag="PLU08_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ70621.1"
                     /translation="MQLLKPELNSLIQKEALDLFYKNGYQATLMRDLAAAVGMSVGNT
                     YRYYKSKHDLFTTLVQPVHEKLVELLEATHDNPEAHFNELCLTTIMTRFTAICSKYHK
                     ESVIFLEYYLAQEDDPVIQNFRGLIYRTVKAHLPQITMPMFDLLYHFIATGTVFVLRK
                     SAPENIVSNLKLLYVFLFKDVKDRINAL"
     gene            complement(19482..20486)
                     /locus_tag="PLU08_02600"
     CDS             complement(19482..20486)
                     /locus_tag="PLU08_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70622.1"
                     /translation="MKLKKFMLAIGVIGLLLTGCDDPNTSSSQTSGSETSEITEAERF
                     SAAGYSPLKGWPVESIRMHYTREGIDAAWVFTNVLTPTGFGQNTDLYVRSYEDELGID
                     VFKSFEVVRVTADATTFTNYVALYDGKTDYTVIVDEEHEFGIEAFSGKVRIGVSFVEE
                     NEAVPSATYFRFTVQEKEEFPGAPKVDRSAHRSEITFSNDFKVTTRSKEIVEWAFSDL
                     YKFKVEVGESSTNVGNIPNNSGVGYLTPQLRIYKDQVLTLNMEQGTMKQVIFHGVNYT
                     EDGVDVKSIDAFPVVEGAQQFKTANAVLYHFDADNTTSLSLVVTANARLLDVTVIYFL
                     "
     gene            complement(20631..>21299)
                     /locus_tag="PLU08_02605"
     CDS             complement(20631..>21299)
                     /locus_tag="PLU08_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70623.1"
                     /translation="EEEEELIVELDLEDPKLEALIRKLANEEIDKRPVPEKEVVVREV
                     EKVVEVKPEPKPEPKPEPKPEPKPEPKPVVVAKPKAEAKPKIERVSFPDRMQMVEDSA
                     KANYNLLKNYLLSYGLNSRVSNVADSFRLGRVLYARLTNSGNSGLKLYLPLDLEDYKD
                     SKIPLKSAEGIKQYEDVPVFLYVRSDLSVKRAQELIDDVMLKHGIARKHDMKEVDHVK
                     ELVK"
     assembly_gap    21300..21399
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21400..22013)
                     /locus_tag="PLU08_02610"
     CDS             complement(<21400..22013)
                     /locus_tag="PLU08_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70624.1"
                     /translation="MQKKRTSAVLTLILAILMLTFFGLAHIVSTVYLVDGAPIGVLGM
                     LVKFKEVLLSLFVPNFGDLMSLAGFVATVVVLGVALVLALVWAIKLLYYRRRGWNLLS
                     PLLFLIAIFLGVLLVGSHTDVDLAFRQPTAQVATAYFFVVGVLMVSLSLHLVVVGLKT
                     AGTKKVASLLEEDLDDEYVVIDEQVEERVVEEKEEEPVVEEKVE"
     gene            complement(22123..23790)
                     /gene="gltX"
                     /locus_tag="PLU08_02615"
     CDS             complement(22123..23790)
                     /gene="gltX"
                     /locus_tag="PLU08_02615"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOQ70625.1"
                     /translation="MSREKLANIIFPEITTTIADLEERYPHRSLKDGAEVTRFAPSPT
                     GFLHTGSLFAALVSRTVAHRSGGVFFVRLEDTDTKREIAGAGENLLNEMKIFKVYPDE
                     GYLGDSELGDYGPYKQSERALIYKTVIKQMIKDDLAYPCFCTTDDLALLRATQEASKV
                     VSGYYGHYATCSKLSADEAIAKIEAGVPYVIRFRSKGNHENRVSFDDEVKGTIEIAEN
                     DQHIVILKSDGLPTYHFAHVCDDHFMRTTLVSRGEEWISSVPLHLEMFAAVKFNQPRY
                     AHLPTIDKLDDGNKRKLSKRRDPEAAVTYFLELGYPVDAVLTYLMSIANSNFEEWWAE
                     NREKPLSEFPFSISKMSVVGALFDIEKVNFFAREYIASLTAKEVVDEYLAYALYNKNE
                     KLVKRIKDNYDLFVQILNIEREGEKPRKDYHNYSMVYPSIKFFFSDEFAAMHEDFDIG
                     EFKESLSKDTLIGLLESFKTELTYGEGNSVWFDSLKKVAEKHGFAINNKAYRTNPELY
                     NGNVADCAEVLRLAIVLSKKSLNLYEVLSILGPKEVEKRLTAMQNKL"
     gene            23910..23983
                     /locus_tag="PLU08_02620"
     tRNA            23910..23983
                     /locus_tag="PLU08_02620"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23942..23944,aa:Gly,seq:gcc)
BASE COUNT         7776 a         4602 c         3684 g         7883 t
ORIGIN      
        1 aagttcaaat tgactaattg ttaaagttga ataatagcta ttttccgtaa ataaccagtt
       61 agagtcacat agatatagat cagtttgtga aacatacact tgcttatatc cataattagc
      121 acctaaaaat cctttagcgt taaaagtaat cgcctgcggg tcagcggcta atttaagtcc
      181 aattatcttt gtcattccgt gcaccggtgt atcttcaaaa taataaatat cgttatatcc
      241 aatattagtt tcagtgccat ctaataaact tatttccgga cgcggatctt cttcatcaat
      301 cgtaatatat tcataaccaa caaagaataa gttattatca attacacggt ggtcgataat
      361 aatattgtcc ttaatattta gttcatacac tacttgatga ttcgtccgtt caataacagt
      421 aattgctgtc gtggcatacc caaaccatcc tggcagccaa aactcttcat attctggaag
      481 taaaacatat tcatactttt cactgataac tacgacctat tccggtagca aatacatccc
      541 aataccgtac gaatccgcaa aagtgagtga tttacttaat gtaatgcttt tatcatcagc
      601 aacatcaaat acttgaagcg cgccgcgttc acgcgctaaa taataaattt gatagccatc
      661 agttttaata atgtcagctt cactaacacc tgccacttgt tcgtttgtac cagtgaaatc
      721 gcgacctttc gtcccagcgc ccgtatcaga ttctggaaca gcatcacccc cagcgattgt
      781 atcgtaatat tcattcgtgg acttgttacc ttctttgact aattcattaa atttttgttt
      841 cgtttttact tgttgcattt tcgttttggt aattaagtcg ctattagttt aacaactcgc
      901 taaacttgtt aacattccca aaaagaatag tgatactata aaccgtgaaa gttttggttt
      961 aaacgatttt ttcatattat tttcctttca gtaattaagt ggtatacact taactcgcgt
     1021 ttaaaacgga ttactatcta ctattatttt gcatcgtttt tgaattttcg tcaatattat
     1081 taaaaaagac actcgaagtg tctttaatta ctagttaaca gattattatc gcgcgcgtga
     1141 ttcgagtaat tctaaagtct tttctaaagg tccagaggcc ataacttttc gcgcttgttc
     1201 ttcattaaaa tatgtcacac ggccgcgacc agtggatttt gatacataca tggcaacatc
     1261 agcaatattg acagcatctt cagcgtcata aaacccgtta ttatgacgat attccgcaac
     1321 accaattgag caatctattt taatgtccgg taattcaact gagcgaatcg ctttttgaat
     1381 acgttttaca agtttttttg tttcatttaa atcaagccct agtgtcataa aaataaattc
     1441 atcaccactt ttgcgataca tgataactgt ttcatccgcg atttcaatta atttccatcc
     1501 tactttagcg agaatctcat cgccaacttt atgaccatat gtatcattta caactttaaa
     1561 gtaatctaaa tctaagaatc cgaagtaatg actatcttcc gtcttatcaa tatgcattag
     1621 taatgtgctg aaattaaata aattcgttaa actatcttta tacaactcaa aaaagagtgt
     1681 ttcaacatta acttttttaa tatcgatttg attaagcatc acaattgata tattacgttt
     1741 aaacatctta ataatttcaa gttgtaaata tcttttagtg cctttaaaat caagtggaag
     1801 atagagcaca aatttagttt gatcgctatt aatttcacgg tgtaattcat cacgatactc
     1861 attagcgccg cgaatatcac atatttgact tttaacatca atatgactta ataattcatc
     1921 aatcgtatca acgcaataag gaagaccgac accttcacat gtaccaaaca gtttaattaa
     1981 aggtttatca caaatgtcat ggctttcttc aataaggaaa aaaagtgaat cattttccct
     2041 taaaaacaca tcatataaaa catctacatt atatttatgt tttagttttt caacccaatc
     2101 cactatgaaa tcacctctta ttaataaatt agtaatttat tattgcacga ttttatgcaa
     2161 aattccataa gtatatatga ataattgaac attttttaca tttgttcaag ttgatgtgtc
     2221 aacatagaca ttttggtatg gggccggagt catatttttt aatgatgaat ggtatcattt
     2281 attattgtgc caatatacat aactaggtaa aattgtataa gcactatcga ttgcttttat
     2341 attaatattt gcataaccgt tataaatttt tattttaact aaaattatct ataagtatca
     2401 cttccaccat aaatagatat tccatcaaat gcatctataa aatcctcaca gtttttaaca
     2461 aaagttcggc agttatcaac atatctataa aaactataat taaaatcagg accaaaacgc
     2521 cccgaataat agtggttatc taaactcctt tccaacgaac gagctgtaaa cctgatgttt
     2581 ttaaatccaa aattccataa aatgtcttgt attcctctaa aaaatttctt gtgtcaactg
     2641 agttatcaac atcccttaga gcattttcaa aagaattcgc agcatttaat aaagtctttg
     2701 cttgtctttc tagctcgtta aaaattcttc taatctcatt tgtatctacc ataactttta
     2761 cctcttttca ttaatcttta aactaattgt aagccaaata aactttaaaa cccaaattta
     2821 atataaaaaa acggcacgca aactgtgccg ttacacgaaa gtttggccta aatggacagt
     2881 tttgatacag tggaacaaaa ccgaacgctt tggaacactg accgaacact attatcatag
     2941 aggcattatt tcagcttttt aagagctttt tctatatcat acgcatcttc aatcttacgg
     3001 aggaattcac tgaagttcac ccatccatca tgatttgagt tatagtcatg atagtctctt
     3061 ccttcaaaga gatagaattg ggttcctgat tgacaaacaa taccaccaac tacttttgga
     3121 tcataaccac gattttggtt tatatcgtat aaataatctc ttaatgcttc gttcttaacc
     3181 gtggtatcag ctaaattggt ttcgtcgttt ggttttgtat caaagatacc gacactacca
     3241 tctttaaact taatgataaa gtctggttgg aaagtattca ttccattgtt ataggaaata
     3301 ccaaagttga ttctcattaa ttcacttccg ttttcccaat accactcaac gacgttttga
     3361 gtatctaaat attggaggaa attcttttcg agatagttaa cttgtccgct agcattcttc
     3421 aaaacgaaca atgggttata taaagatttg attcctaaaa gagttgtgtg ggtttcagac
     3481 gaataagccc tgctctcctc aatcttaaag tcatatctaa cacctttttt accagcgttt
     3541 acagaaagca tttgtctaaa ttctgcagtt gcttcagata taacttcagc gaataattct
     3601 ctgttattaa caacgatttg catgtatctt ttgacttttt ctgctctcgg gaatcctttg
     3661 atgaatgtgc ttaaaacatc cataatcgct gtatttattg gtgacttaga tctaacatag
     3721 gccaagccat ttagattgtc tctaataatt gcattatact gagcttgaac atcattttca
     3781 gacatttgaa cattaacagc gttgccggtg atggttcttt cttccgcgat gttttccata
     3841 cctaaattag tttcttcaag gataacatca ccagtatttg tttctaattt gagaccagca
     3901 tcttctaatc tatttggatc gttgaataat acgttgtttc tatcttcttc agtcaaaccc
     3961 attctcttca taaaagattt gaataaatat tcaccaaatc tagagtcggc agagttatag
     4021 tcctcttgtc tggatctata gaatgaaatc aatttagttt gagcccatac tcttagctga
     4081 gtgtaacctt ctctcatttt ggtccactcc gttttaattt tgtttgggtt atatgaatct
     4141 tgcttggttt cgaatgtcca aatatttgtg aaaatatatg cattatcaag caatggatca
     4201 tcataagatt tggcttctgc agttcttaaa atacgaccaa ttgtttgagt ttcaaatgtt
     4261 tcagatttgc cttcacggaa cttaactaaa atatgtgctc ttggacaatc ccatcctgtt
     4321 gctaccgcag ttttgaatat taaatattca gtgatgtcgt tgttctttct aatctctttc
     4381 ttatcaaaag gatcatgatt gtcgcaccat agctttaatt tgccgtttgc ttctgtgatt
     4441 cctttttctc ttaagaaatc tttgataacg agtttctttg cttcgccctc atctacatta
     4501 gggatttgga taaggactag cggattaaca tttatattta actcagcata tttttttact
     4561 atttcttctc ttttagcaaa gcctttttcg agaataagaa gttcactatc tttctcttct
     4621 aaagatttat cttccttttt aatgccctcg ttaacgatga catcttcttt aatcatccct
     4681 tccttaataa catcttcagg attaacttct acatcaatgt gatcgttaag tggggtcgca
     4741 gacatttcta gtaaaatgct tggaataatg attgtattga tgaactcttg aattctggct
     4801 ttcgcagaag aaccaatatg agattcatca acgaccaaaa caaccttagt accattcttc
     4861 ttggtttgat caataacatc aatgaagttc ataccttctt ggtctttcat taagttattg
     4921 gcccatgcac cagttccctt atccttttgg ataagtttct cccagttgat gaacacaatt
     4981 tctttatttt taatgaattt tctggagcca aagaagtctt cttcaagcaa agagcaaaca
     5041 ggattaccac ctaagtatgt tttaactgcg tcatgagatt gcttgtgaag ttcacccttt
     5101 ccaggacaag cccaaagaat gcagaaatta atgtcgctgt gttcgccagc taattcctcg
     5161 aacaatgatg cagccataaa ggttttacca cttcctgttg gtgctttaaa cactacttct
     5221 tttctatttt ttgatcgaaa gcctaattcg atgtagccct ttaattcatc tactgcatct
     5281 ctttgataat tcttaagtgt ataaatcata actattcatc cctttttata tcttcaacaa
     5341 tctctttgta tatttcatat atctttgatg gaattggttt tagaacaacg tccattccct
     5401 caattagaga gttatcaaca ttgttatcgc ttgagtaaac ataaacgatt ttttctcctt
     5461 gagatgccaa aaccctgctc ttaaactctt caaatttttg agcattgtaa taatcgttat
     5521 aaatgaataa gcgcttgtca ccactcttaa aatgagatga ataatcattt ctctcaactt
     5581 cgtcaaaaat gttttcagcc aaacataata aggcgtcaac tttttctaca agattatatt
     5641 ttgcttgttc ggtattggcc tcgtttttaa taaaatcagt cttatagtaa taaagacttc
     5701 cagacaaacc atcgctgtat tttgaaccgt cttttttaat acccgtaata acggttttaa
     5761 gtcttggata agttatagtt tttgcaatat cgttttcttc agctgattga caaagaataa
     5821 agcgtctctt tccgccatct tctgcatttg ccattaaaac agcttctccg aatgaaccag
     5881 aaccagcgaa aaagtccaaa cataaagcat ctttgttttt gactgtagat attatcttag
     5941 ataataattt aagtggcttt ttaccatttt ggtaatccac accaccctct ttatttaagt
     6001 tgatggtgct tatgtcggtc catagatctc cttcgaaagt tgttgtgtag tctgacagga
     6061 aaagcatttg tttaccttcc caccaaatgt attcaattcc tgtagcacta atgatttttt
     6121 gaaccggctt atcacattgg attttttcaa agctcttatt gtttgttcta taaatcatcc
     6181 tttcggcatt atctaattta aattggagaa tttctctttc ggttaaattt gatttaccaa
     6241 ttctcttaag atacttgtca agaggaagaa tctcccattc ttcgacaggt ttgcttttat
     6301 cgacaataat tttactgtag tacttcgcat atttagcgaa atcgtctaaa ttatctagtc
     6361 tgttattttt tataggattc attgaataat agaacctatt ttttgaataa accaaacaat
     6421 attcttttag cttaggaagt ttcttttcaa cgtgtgacat tttcactcca gacatttctg
     6481 acattttcac aacgacacaa ttaacaaaat tttgtggtcc aaagacttgg tcacacagaa
     6541 tctttaagtt agcttgttcg ttatcatcaa tagaaataaa aatgacgcca tcttgtttca
     6601 aaagattcct agcaagagtc aatcgttttt gcatgaaatt tagccatttg ctatgcaaaa
     6661 aagtatcgtc gctatttatg taattatcgt tatataaaaa gtcttcacta ccggtgttat
     6721 atggcggatc aatgtaaatc aaatcaaacg aatcttgaag aacataattt aagcaagtga
     6781 gagaatgaaa gttgtcaccc tcaatcaaac agtgagatag gccgcctttt ttaatgttca
     6841 ggcttttgat tggcgagagt gttggaatgt atttttcaca gagcaacaca acttcttctt
     6901 ttgtgttttc tttatcccaa acaagaccgt attttttaga cgcctttaat tcggattcta
     6961 actctttaat ttgctttaat aattgctcaa tatcaaaatc agccatagtt caacctcata
     7021 gattagtaaa atcaaggttt ccttgaattc acactatatt ctacaaagtt taacttgatt
     7081 tgtctatatt caaatcaagg tttctttgat tatttgtcta agaggaaaag ataaatgaga
     7141 ttgagagaac taagaaataa gagcggtcta acgcaaaacg aaatagctaa taagcttggt
     7201 gtttctgggc aaactatact taattgggaa aatggtattt atgaaccaaa gattaaccaa
     7261 ttaatagaat tagctgattt atttgatgta actgtcgatt accttatcga aaggaaaaca
     7321 agctataaaa gcgttgatgc tatttgtaaa gaattagaac gaattcccaa agaaaacgtc
     7381 attaacttta ttaaagaaga gcttcaaaaa ttatagttca atcttctcaa ttgttgagcc
     7441 ataatcacag gctctttttt ccattctaaa aatgctttct tcaagcttgt atttaaaggc
     7501 aattcttctt tcaatctcta tgtactcttc gattgtgatc tcacccttat ctaacaagcc
     7561 ctcaaaataa accatagcta caatgtaatt atgtactcta gttacatatt catgatttat
     7621 gccttccatc ttagtggacc tcctattatt tccactaatc gctctaaatc taaaaaatgt
     7681 gaagaaaaac attcctaatg gtgaatagtt gtattttttt gtatcaaaat actgtgaata
     7741 actaccctac aggcgaaata tattgcggaa aaatatctca aaaaagtgcg atgtaatcaa
     7801 agaaaaaagg ctaataccga tatatattaa ccttcaattt ttaagttggt gacccgtacg
     7861 ggattcgaac ccatgaatgc atgcgtgaga ggcatgagag ttaaaccgct tctccaacgg
     7921 gccataatcg cgattattat tttagcactt acaaaataag tgtgtgcaat gaaaacaaaa
     7981 aaacggcact ttatagtacc gtttgattta cctatggcga cccgtacggg attcgaaccc
     8041 atgaatgcat gcgtgaaagg catgtgagtt aaaccgcttc tccaacgggc cagctggcgc
     8101 ctagtgtagg atttgaacct acgaccgatc ggttaacagc cgattgctct gccgctgagc
     8161 taactaggca tgttagacct aggcagtcat aagaatatca cacttcctaa gtcttggcaa
     8221 taataattat tattattatt tagttttacg ctcaatttca gcgacaacat cagcgactgt
     8281 ggaaaatcca agcatttcgt cgttactaaa ttcaatacca aactcttctt cgagtctcat
     8341 catgacttcc actaaatcaa ggctgtcaag tcctaatgct tttaattcca tttcaggttc
     8401 aattttttca accttgagca attcggtgaa tatttgttca atacgttcta aatttgtcat
     8461 agtattctcc ttagaattca aattttaaca aatagcacgc attatttcaa ataaaaacga
     8521 ttccaaggtt tcttcactgc taagcgacga ataaaagtta attaaattac taacgcgctt
     8581 tcgtgactag caatttctga aagttgatta ttaactgatg ctttaaaggc cgcagcaatt
     8641 gtggcaaaaa taacaacaac aagtaataac cctaaaattt gtccttttag ttctgacata
     8701 aaattacctc cttaattata aataaccaac aattgttgtt cttgtcacat aaatgttaat
     8761 gtcttcattt ttaataataa tcggagtgta tgttttaatt aattgatatt taattgtttc
     8821 ccctacactg atgtaagagc attcgccttc acaagtaatc gttatgcctt cttccgctag
     8881 ttcgttaact agacttggcc taatgccccc ttcttcacta ataatatagc taacaattgt
     8941 tgcccttgtt tctagttcaa caatgatttt cccaatgatg aaaaagtcaa caccaagcaa
     9001 aattgtcggg aaaattagga tgaatgaata aacaaggctc ataatattac tcatactata
     9061 tattctccaa tcatccccat aatacttatc atcagtgtca taatcataat tcctgtacca
     9121 ataaataaac taatgttaaa gcgctcataa ctactttcat gtagcgctat ttcttcatca
     9181 ttatgttctt tcactagtgt ttcaaataaa taattaaatt gccacaacct ttttggatca
     9241 acaccgtgat taacaagcat ataaacatta atcataattt gcgaaacaag cggatgctta
     9301 aagggtaagg aaaaattaat aaaaggggta acactatgat cattttctaa agcattaatc
     9361 aatgattcta aaagagcgga catcgcacca cttgtcgtcg ttaatgtaat atttaaagcg
     9421 cgatatacat ttaagttatt atcaagcgct acacgaaaat gggtgaatac attaataaaa
     9481 tccgctaaag tgatattact cttttgcttc tgggggataa aggtaataac aacaattgta
     9541 aaaccaacac cagcaaatat cagtgcaagt gttaagtttt tcttccagaa atataaaata
     9601 gcgccaacga tgattaataa taacccggca ctacgttcta accatttaac taacacggcg
     9661 tgttctcctg attctatttt caaatactaa aacaatgctt gttaaaaata tagcgataaa
     9721 gacttccata cccgcaacat aaattagtgg taatgtttca aattttaaaa agccatcaag
     9781 cgctaggcgg aagacgattg ctactaacat aataaaaaac caattaaaaa taaatttaat
     9841 aagataagca ttatcaattc tagttaattt aataagttgc gtttcactat tagaaatact
     9901 aaaaagcagt acttccgcta ctttaagcat ttcaccaccg tgttcattaa taagtttcac
     9961 catctcaaga aatgattcat aatattgatg agcaaaagta agtcgcagtt tctcaagtaa
    10021 aagcatcccg tcttcattca ttgttaaata tactcttgat ttttttacgt caagcagtgg
    10081 atatattgta gttagtgttg cttctactgt tttttgaatg tcaaagttaa gaataaaata
    10141 ttccataaaa ttaaacataa agcgtctttt ttcaatgtct ttgcgataat tagtaacgcg
    10201 atatgtaaga aaagcagcga caactagtgc aattagtgca ttgataatcg ttatttttat
    10261 accgcctagc actagggcac taaatattgc tattactata accaaaaaga aagcaccacc
    10321 ataaataata atcacggaat cacctcgtga taaagtattt caatttcatc agtgtcaaca
    10381 ttgtattcag tgattttttc gacataacga taaatcgagc catctgcctt atattttttt
    10441 gctaaatgaa taacaaacgg aaaagcttgt aataaactat gacgatcatc atgctttgct
    10501 aaatagatga cgcgttcgta tgctaaagct gtactatcag cgtggagtgt tacaatattt
    10561 ggatgaccgc ttattgctcc ttgcagtatt tctttcatct caccaccccg actctcggca
    10621 ataattaaat aatcgggatg aaaccgtaaa gccttttgga taaaactttt aatttcttct
    10681 tctttatttg ttaatgattg ccaaagtgtt aaatctaaat ctttatttaa tgttgtgact
    10741 tcacttaatt caaggacatt atcaatcact aacacccgcg tattagcggg taaaagcatt
    10801 aataaatact tttgtaaagt cgttttaccg actccagttg tgccgctaat gatgatggag
    10861 cgattttctt tcatcacata ctttagaaca tcaacaacat cattatttaa ataatgcgga
    10921 ttacttgcga gaaaattatg attaatgcgc aaggcaaaac ttaaactttc ttcaaattca
    10981 aatctactac atgcataatg catcgcacta aggcgatact ttccaattga tatatcaaga
    11041 aatgtatcag cgtaagaaaa tctctcatta gcaaaattag cgacattacg taaaaagctt
    11101 gcgacagtct tatgattaat ttcgccatat aacttgcgac cactctcatt tgaaaagtaa
    11161 aataatttgt gaccgttata actaatatct gttatgtttt tatcactaag gagcggggct
    11221 aaaaaactct cttcaattac tttaattact ttttccattg ttaactcaca taataggcaa
    11281 atgttttatc aaaagtcaca ccaaagccta gtggacacac gaggttaatt gtaaaatatc
    11341 gacttctttc actttcttca ctaacgactc catttgtgta aaatgtaatc gtatattcgt
    11401 aattttctct tttcatatta aggttaaaat gattagcgac tacaactctt acaagcggtt
    11461 tttcaaagta gagttcataa gtaactaaat ccattgttgt cacatgataa atatgatcat
    11521 gagttacaga actaagtact tcattaacat ggtgatagcg aatatttatc gctagcagtg
    11581 tgctataagt aaataaaaat aaaaaagcaa aattactcca tgccatacta gtacctatta
    11641 attattactt catgttcacc gcttgtgcca aagtaagggt gatctatttg aatggtaaat
    11701 tcgtatttta gattaaattt actataacta aaatctttga tctctaatgt aaacttcgtg
    11761 ttttgtaact catcaaaatg atacttagga aagaaatatg tatcggtatt tagatattcg
    11821 gcactatatt tactacgcgc aatttgtagt gtatctgctc tcacaaacaa tggtgttttc
    11881 acactcgtta ctaaacgatt ataagcacgt gttaagttta aatactgcat tgctcttccg
    11941 ccaacattag caagataagg aaagagagct gggttgctac aatataaagc cgcactgcta
    12001 taaaaatttt cgttatcata aagcacccca attgataaaa aaagcccact aaaatcaaaa
    12061 tataagtcat gattaaaatg tttaataggc gttaaattag cggagctaaa actcatcttc
    12121 cgaaatatat cactgcgatt agtactatca tataaataac atacttcagc ttctaataat
    12181 tgccgtgccg cactaccatt atttaaatta acattgacac ctttttgata gccataaaaa
    12241 tatgaataat gacgacgctc tgcttcactt gatgttttgt aatataaaat aatacgattt
    12301 tgaccaaccg taaactttcc tgctgtagca cgatatgtga ttagcacccg aacattacgc
    12361 agtgcccttg tcgatgttga tggttgagta taaacaatct cattagcgct attgtatacg
    12421 gtatagcggt atgttaatgt tgtcgtgtaa gaagcaataa aaggaatcgc cacttcataa
    12481 ctctgtgtaa tatctccagg attattacga taactaccaa gcgctgaagt ttgaaaatta
    12541 ctttcaggta caagcggaat aaacacgctc ataaaaatcg ttaaccacaa tgttttaaag
    12601 tatctcatgg taataaataa aagagtattt tgaacttttt cacatttacg actgattctt
    12661 cgtgcaatta acaaaatttt attttattat ttatgtatga ttgtactaat gggagcaagc
    12721 gcaagcggaa agacggaagt tgccaaaatg cttggcaaac tttttagtat taaaaaagtt
    12781 gtgacgcaca caactcgtga aatgcgtgtt aacgaagtta atggtcgcga ttatcacttt
    12841 gtcacaaaag aagaattttt acgacttaaa gctgaagact actttgtaga gactgtagag
    12901 tataatgata acttctacgg aacttcaaaa gctgaaatag ccatgaataa agtattaatt
    12961 attgaaccta ttggtttaaa atcaataaga gccttaaata gcgaacatat cgtcgtcttt
    13021 ttcatgcaag ctagtagagc tattcgccgt aaacggatga ttaatcgcgg tgataatcca
    13081 aaaattgcca ctgaacgtat tttaatcgat gacgctaaat ttaatccaaa aaacatcgaa
    13141 ggtatcgatt atacgattga tagtgaaaat caaagcataa aagaaattgc gattgaagta
    13201 tatgaaaaat atgccttaaa actcgttaat tttaaataag ctttaatgtt tctgttatta
    13261 agtaaggtag atgagataaa agtttctcgt ctattttttt aatacgcgga atcccgttaa
    13321 tttcaaggaa cccacgcggc actaaaattt tcccatttaa atctttatct gtcgttagcg
    13381 ccacaagggc accaaggacg gctttttcgg gaggatgaaa aaacacactc ataaatctgt
    13441 ggacaatctt gttaaagaat ttccatttga agcgtgcgat attactaaca gctattcctg
    13501 ggtgataaag ataaatttta cctttatttt caaacggaat tagcggagac ttagtcatta
    13561 agccaagcac aaatattttt tgggcagttt tcgcaacagc atattgtttc ggccgcgtga
    13621 tcttttgtcc gcttattgcc cgtgcaagta tttgcgagct aaacttacgc gaatatgctg
    13681 gactagaaat ataaattaac cttgtttctg cactaattaa cccgtgattt gcaagtgttt
    13741 gtgtaaaaat aaagtttcct aagtaattaa taccgaatgt aagcgcaaca ccatcttcag
    13801 tcttagcatc tttagcaggc gaataaatgc cagcattaag cacaaagccc tcaggcttaa
    13861 tattgtgact aattaaacta ttaacaaagg cttcaataga atccttttta ctttgatcaa
    13921 aagggagtac cattacactc gcagcaggat ataattcgcg tatcttattt attgcgtctt
    13981 cactccgttt aggattacga ttagccataa taacgcgtgc ccctttactt acaaggagtg
    14041 tgctaagggc aaaacctaaa ccactattag cgccagtaac gacataagtt ttatttaata
    14101 aagacgggat tcgttctaaa aacttttcaa cgtacattaa tcttcttcac tttcaacgat
    14161 aacttcaact ttttcaacaa caaagccttc aacttcaatg attttgacag ttattttttc
    14221 ataagtgaat tcgtcgcccg ccttagcaaa acgatcgagc atttcaatac accaaccagc
    14281 gaccgtatca taatcattat cttcatcttc gcttaattca aataattcaa agaagtcttc
    14341 aatatttaag ccaccatcaa taatgtaatg attatcttca agcttaacaa tcggctcatc
    14401 aacttcatca gcctcatccc aaatttcacc aataatttct tcaaggatgt cttccattgt
    14461 aataataccc tcggtgccac catactcatc aatgacaatg gcaatatggt ttttcgtttg
    14521 cttgaattcg gttaaaattt gactaacatt tttcgtatga tggacgaaaa gtggcggttt
    14581 aattaagctt ttcacattaa ttttcttatc ggaaagtgaa agtttaagta agtcttttgt
    14641 ccgtaaaata ccaataatat tatcgattgt atcttcgtaa acggggatac gactatattt
    14701 aaaaatatct tcttctttca ttaactcttt aatgtcatct tcaatatcat aactaaagac
    14761 atcaacacgc ggcgtcataa tttcgtaagc ctctgtttcc gtaaattcaa gcgcactgcg
    14821 cactaagtca cttgtctctt cgtcaattaa gccttgctct tcaatttcat cgacaataat
    14881 ttgtaattcg tcttccgtat aaatttcatc gccatttact tcttcattac cattcttttt
    14941 cttccggcgt gcactaatta gtctaaacgg aaataaaagc ggcgtaaata aataataaaa
    15001 gaacataaag atattcgcgt aagcatacga catttgataa tttaatttaa cagcgagtgt
    15061 ttttggcaca atttcaccaa tcgttaaaag gacaataaat aaaacaacag agaaaatagc
    15121 tacatattgt ccagtagcgc catgcgcatt atcataggct agcgccatcc ctgtcacaag
    15181 ggcggtaact gcaatattta caaaattgtt accaagtaat attgtttgga cagtattctc
    15241 ataattcgta attattctca gcagtgtttt gctacgcttt ttaccgtctt ccacatcacg
    15301 ttccacacga agccgattaa tcgtggaaaa agcactctct gaactcgaga aaaagaaact
    15361 aagcgcaaat aaaagaataa caattgcaaa tctaattaca ttacccatta acgcttgctc
    15421 ctttaacagt accacttgtt tccgcaattt ggccgactaa ttcttcaagg acgtcttcca
    15481 ttgtaaccat accaagtagt ttctttttat catcgacgac aaaagcaata tgcgtgttgt
    15541 tcttcttaaa gccttcaacc attttagtaa gcgtgatctt ataattaaca taatatgggc
    15601 gtgccataat tgtgcgtggg ttaatatttg gtttcttttg ataatgttta atatattccc
    15661 gcactaaaat aacgccaaca attttatcaa gtgaaccgtc atacagcgga attcttgaaa
    15721 aatgtgttga tagcacgaaa tctttaaatg aatctttatt aaatttacta atgtcatagg
    15781 caacaatcgc gtttttcggt gtgataacat cttttaccac tgtttcacta aattcaaggg
    15841 aggagataat aatatcacta gcgtcttcat taataatccc gtgctcttca attgtttcaa
    15901 tgacttgctc aaaatcatca gcggtgaaaa tattatcttc tgtggctttg aaaagttttt
    15961 taacaaccca gacaatacca cggaaaaaaa gcgcgacagg atagctaata ataataaatg
    16021 gcactaaaag ataagcgcta attaatgcgt attgttcact atagcgacgt cctaatactt
    16081 ttggaatcgt ttccgcaaat aaaaacatca gcggcacact aattaaagtt gcaagtaatt
    16141 caccagtgtc attgccgtac tgattaacaa ggacaacagt taaaagcgca gtaaaaagta
    16201 agtgcgctaa attattaaac acaacgacag caataattga attttctagt ctatttataa
    16261 cccaagcagc aagtttagcg ctcacgctac cttcttctgc tttggctctt attttaaagc
    16321 ggttcgcagt cataaacgcc gtttcagatg aactaaagta catcgaagcg acaaaatata
    16381 atattaagag aacccacgtc tgagggtcat caatcattta gaaaccacac tccttcttta
    16441 taaatgtgca tattcatatg caacgatagc atgttatcac agcgccgaaa tttgtgcaac
    16501 gctaaagtta actaagcaag gttgggtctt gatcactatt actataacct tcatcttcaa
    16561 taataacccg tccaactttc gtcccgccct tataaaagcg catcttccat gttgtttttt
    16621 ccacaacttt atcgaagata tatagcaact gtggtaataa cacaagcaca aggataccac
    16681 taagtgctgc accacggcct attagttcac caagcgagga cacgccttca atacttgaga
    16741 ggaattttag actataacca gcagaggtca aaattaagca cgatgttaaa atactatgac
    16801 ccccgctatc aagggcatac ttaattgcat cacgtctaat catcgttttt cgtccctcag
    16861 tgtaaaatcc agtaaaaagt atagcgtaat caatcgtcgc cccgagttgt accgaactta
    16921 caatcatata accaataaaa atcatcggtt gactcgttaa atacggaatt gccatattaa
    16981 tccaaatcga aatttgaata acaagcacta aaataaatgg caatgagata ctgcgaaaac
    17041 taacaagtaa tatgataaat acaaagccaa ttgaaattaa gtctacaatc acatagtcag
    17101 tctcgacaac attacgaatt tctttaactg atggcgagac accaataata tgtgcatcac
    17161 gggcaaaagg ttgacgggcg acaacagttt caacaatatc aacagcaatt tccgcccgcg
    17221 gtgactcact ttcagtgtta acagtaataa taatgcgtga ataatgttca cttaaaaact
    17281 gtgccttata ttcttcggta ataagattat ccataatatc ggcaaaaata tcatcaagga
    17341 aatttttaat cgtttgatct aaatcatcgt agttaggaat ataagtctca atatcactta
    17401 agtttgaaag actcgtaatt tgtacaacaa aatcttgatt agcgcgctca aactcgcgat
    17461 aaagctgttt aatcatcgct ttttcttggg caatataagc atctgcttca tcgcgtggta
    17521 caagaatagc cattaagttt tctttaccaa acttcgcttc aatccgattt ttctcaattg
    17581 ccgctggtgt gccttcgctc acactcatcg ctgcttcacc atacacaaaa ctattattag
    17641 tttgtgctaa gtaagaaggc acaattacga gtaaaagtat cgcaggcaca acatagcgta
    17701 attttacaat ccatttagtg agtccaccaa gaagtgggaa aaagcgttta tgttttgttt
    17761 tttccacaat tttatcagtg agcaagatta aaccaggcat aaaggtaaag gttgagataa
    17821 tactaagcaa aatcccttta atcatcaccg ccgcgaggtc aatcccgatt gtgtaacgca
    17881 taaacatcag tgctaaaaat ccggctgcag ttgttaacat gctcgagtta attgacacaa
    17941 atgacttgcg cattgccatc accattgcgt cttttttatc aagtccctct gccctttctt
    18001 tttgataagc atgcaagaga aatatggaat aatccatcga gattgctaat tgcaagagtg
    18061 aggctgtcga attcgttaaa aaggatacag acgggaaaaa taagtttgta cccatattta
    18121 ccaaaacagc gaccccgacg gcaattaaaa agataatagg ctcggcccaa cttctggtaa
    18181 acactaataa aataaataag atgattggga caacaattac cgttacccgc aatacttccg
    18241 attcagttaa cttctgcgtg taataagcat taactgctgt tccgcgcatt gcataaggtc
    18301 gcttgatacc ctcaagatag gagcggattt gttcaatggc cttgccgaca gcgcgactat
    18361 gatcttcttc cgtaaacaca atttgcagca aagcactttt atctttataa aaagcgttaa
    18421 gccccggaat atttgcgaag tcaaagacac cggacatccc attttcttca gcaatgcgtt
    18481 gaatatcatt aatcgtatcg cgttcgacga aactatcaat ccacacaact tccataaccg
    18541 ttggaatact tataatttca tctttataca cgaacacttc ttgtaatgtt tcaacttttt
    18601 caatcattac actagcttga ccagtaatgc caaactcttc ttccatcaca cgtaaagatt
    18661 ctttaacacg cgtatcttct ggcaaatatt ttgtcgtgtc gtaatttatt ttgacatggg
    18721 gaatcataaa aatactagcg attgttaacg ctatccagaa accgaaaaag attactttga
    18781 agcggacgat gaaattaaat atttttttca taatgcatta atcctatctt tgacatcttt
    18841 aaataagaac acataaagta attttaaatt agatacaata ttctctggag cagattttcg
    18901 caaaacaaaa acagtgccag tcgcgataaa gtgataaagt aggtcaaaca ttggcattgt
    18961 aatctgcggc aagtgtgctt ttaccgtgcg gtaaataaga ccacgaaagt tttgaataac
    19021 tggatcatct tcttgcgcta aatagtattc aagaaaaata accgactcct tgtgatactt
    19081 agaacatatc gcggtgaacc gcgtcataat cgtcgttaaa caaagttcat tgaaatgcgc
    19141 ttccggatta tcgtgcgttg cttcaagtaa ctcaacaagc ttttcatgta ctggttgtac
    19201 aagcgtagta aataagtcat gtttgctttt gtagtagcga taagtgttcc caacactcat
    19261 ccccactgcc gctgctaaat cacgcattaa agttgcttga tacccgttct tgtaaaagag
    19321 atcaagcgcc tctttttgga ttaatgaatt aagttctggt tttaaaagtt gcataatgta
    19381 aacacctgtt cactaatatt gtatagcaat gctaaaatcg ttgcaagaaa aaaataaaaa
    19441 gcccatcaaa tatgggcttt ataatgtaaa taattaatat tttataagaa ataaataaca
    19501 gtaacatcga gtagtcttgc attagccgta acgacaagtg aaagactagt agtgttatca
    19561 gcgtcaaagt gatataaaac agcgttagcc gttttaaatt gttgagcacc ttcgacaact
    19621 gggaaagcgt caattgactt aacatctaca ccatcttctg tataattaac accatggaaa
    19681 ataacttgtt tcattgttcc ttgttccata tttaaagtta agacttgatc tttataaata
    19741 cgaagttgtg gggttaagta gccgacacca ctattattcg gaatatttcc aacatttgta
    19801 cttgattcac caacctcaac tttaaacttg taaagatcgc taaatgccca ttcaacaatt
    19861 tcttttgatc ttgttgttac tttaaaatca ttagagaaag tgatttcaga gcgatgcgca
    19921 ctacggtcaa cttttggtgc gccagggaat tcttcttttt cttgaacagt aaagcggaaa
    19981 taagtagcgc tcggaacagc ttcattttct tctacaaaac taacaccaat ccgtacttta
    20041 ccgctgaaag cttcaatacc aaattcgtgt tcttcatcaa cgataactgt gtaatcggtt
    20101 ttaccatcat agagcgcaac ataattagta aaagttgtgg catcagcagt gacgcgcact
    20161 acttcaaatg atttaaaaac atcaatccca agttcatctt cataactgcg aacataaaga
    20221 tcagtatttt gaccaaatcc tgttggtgtt aaaacattcg tgaataccca cgctgcatct
    20281 atcccttcac gagtgtagtg cattcttatt gattcaactg gccagccctt aagcggtgaa
    20341 taaccagcag cgctaaatct ttcagcttct gtaatttcgc ttgtttcact accagaagtt
    20401 tgactgctcg atgtattagg atcatcacat cctgttaaga gtaagccaat tactccaatt
    20461 gctaacataa atttcttgag tttcatacat aacctccgct atcaagttat attatcatac
    20521 ttcatttaat attttaaatg atagagtaag aaagcggata cattattaca ctttatatta
    20581 aaaaagccta tgcaagcata ggctaattta tgtttattta ctaattaatt ttatttaact
    20641 agttctttaa cgtggtcaac ttctttcatg tcatgcttac gggcaatgcc gtgtttaagc
    20701 attacatcat caattaattc ttgagcacgt ttaactgata agtcactgcg gacatataag
    20761 aagactggta catcttcata ttgcttaata ccttcagcgg atttaagcgg aatcttacta
    20821 tctttatagt cttctaaatc aagtggtaag taaagtttaa gtccactgtt tccggagttt
    20881 gttaagcgtg catataggac acgacctaag cggaatgaat ccgcgacatt agaaacacgt
    20941 gaatttaagc cataacttaa taaatagttt ttaagtaagt tatagttagc ttttgcggaa
    21001 tcttcaacca tttgcatccg atctgggaag gaaacacgtt caattttcgg ttttgcttca
    21061 gcttttggct tagcaacgac gaccggtttt ggctctggtt tcggttctgg ctttggttca
    21121 ggtttcggct ctggttttgg ttcaggttta acttccacaa ccttttcaac ttcgcgcacc
    21181 acaacttctt tttctggaac tggacgcttg tcgatttctt cattcgctaa tttacgaatt
    21241 aaagcttcga gtttcggatc ttctaaatca agctcaacaa taagttcttc ttcctcttcn
    21301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cttcaacttt ttcttcgaca
    21421 actggttctt cttctttttc ttcaacaacg cgttcttcaa cttgttcatc aattacaaca
    21481 tattcatcgt ctaagtcttc ttctaataat gaagcaactt tcttcgtccc agcagtttta
    21541 agaccgacaa ctactaagtg gagtgaaaga ctaaccatta agacgccaac gacaaagaag
    21601 taagcagttg caacttgcgc tgttggttga cggaatgcaa ggtcaacatc ggtgtgactt
    21661 ccaactaata atactcctaa aaagatagca ataaggaata ggagtggcga taataagttc
    21721 caaccacgac gacgatagta aagtaattta atcgcccaaa caagtgctaa aacaagagca
    21781 acaccaagga cgactactgt agcgacaaag cccgctaaag acataaggtc accaaagttt
    21841 ggaacaaata atgataatag tacttcttta aatttcacta acattcctaa aacaccaatc
    21901 ggggcaccat caaccaagta tactgtcgaa acaatatgcg ctaatccgaa aaaggttaac
    21961 attaagattg caagaataag agtaagtact gcactagtgc gttttttctg cataataaaa
    22021 ttgtcctttc attaatttta attactttca taataataaa aacacacgca aattgcaata
    22081 gaaagtatgc aaaaatgata tattttgttg aaaaatcagc tattatagct tattttgcat
    22141 tgctgtaaga cgtttttcaa cttcctttgg acctaagatt gaaagcacct catacaaatt
    22201 taggctcttt ttgcttagta ctattgctag acgtaacact tctgcacaat cagcaacatt
    22261 accattatat aattctggat ttgtgcgata tgctttgtta ttaatagcaa aaccatgctt
    22321 ttctgctact tttttaagcg aatcaaacca cacactatta ccctcaccat atgtaagttc
    22381 agttttaaat gactcaagta aaccaattag tgtgtctttc gataacgact ctttaaattc
    22441 accaatatca aaatcttcat gcatagcggc gaattcatca ctaaagaaaa acttaatgga
    22501 aggataaacc attgaataat tgtgataatc ttttcgcggt ttttcgcctt cgcgttcaat
    22561 attcaaaatc tgcacaaata aatcataatt gtctttaatg cgtttcacta atttttcatt
    22621 tttattatat agagcgtatg cgagatattc atccactact tcttttgcag ttaatgaagc
    22681 aatatattca cgggcaaaga aatttacttt ttcaatgtcg aagagcgcac caacaacact
    22741 catcttacta atgctaaagg ggaattcact aagtggtttt tcacgatttt ctgcccacca
    22801 ctcttcaaag tttgaattag cgatgctcat caaataagta agaacagcgt caactggata
    22861 tcctaattct aagaaatatg taacagcggc ttctggatca cgccttttac ttaatttccg
    22921 tttattacca tcatctaatt tatcgattgt tggtaagtgt gcataacgcg gttgattaaa
    22981 tttaactgct gcaaacattt ctaaatgtaa tgggacacta ctaatccatt cttcaccacg
    23041 tgagacgagt gttgtacgca taaagtgatc atcacaaaca tgggcaaagt gatatgtcgg
    23101 taatccgtca ctctttaaaa taacgatatg ttgatcgttt tccgcaattt caatcgtacc
    23161 ttttacttca tcgtcaaatg aaacacgatt ttcatgattt cctttgcttc taaaacgaat
    23221 aacataagga acgcctgctt caattttagc gatcgcttca tctgcactta atttactaca
    23281 tgttgcgtaa tgtccgtaat aaccagagac tactttacta gcttcttgtg ttgcacgtaa
    23341 aagcgctaaa tcatcagtag tgcaaaaaca agggtaagct aaatcgtcct taatcatttg
    23401 tttaattact gttttataaa taagagcacg ttcactttgt ttatatgggc cgtagtctcc
    23461 taattcacta tcacctaagt agccttcatc agggtatact ttaaatattt tcatttcatt
    23521 aagtaaattt tcaccagcgc ctgcaatttc acgttttgta tcagtgtctt caagtcttac
    23581 aaagaacacc ccaccactgc ggtgcgcaac agtgcgagaa actaaagccg caaataaact
    23641 accagtgtgt aaaaatcctg taggtgaggg ggcaaatcgt gtaacttcgg caccatcttt
    23701 taaactacga tgcggatatc tttcttctaa atcggcaatt gttgtagtaa tttctggaaa
    23761 tataatattc gctaattttt ctctgctcat aattaacctc ttttaacaca tataaatata
    23821 ccatactatg tatatttttc atcaatttta ctaatttata aggacattaa ttgaataaat
    23881 ttcataagag tgataatata atcaagcacg caggtgtagt tcaatggtag aacactacct
    23941 tgccaaggta gatacgcggg ttcgattccc gtcacctgct ccatctaact atgaattagc
    24001 ttccaaatct tggagagagg tataatagaa atacccaccc aagaa
//
LOCUS       DAOSTA010000016        18920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_3064_length_18920_cov_10.558760, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000016 DAOSTA010000000
VERSION     DAOSTA010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 18920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18920
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3064_length_18920_cov_10.558760"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            366..1904
                     /locus_tag="PLU08_02625"
     CDS             366..1904
                     /locus_tag="PLU08_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOQ70626.1"
                     /translation="MKFLKNLKEALISSLPLLAVIILVAGIIFPVKTNTEIIQLVVGY
                     TGVVIGQAIFLTGLDYSVLPIGNLIGSSIVKLNKLVFVLMFAFLFSLLAAVAEPGITV
                     LAAQVGGIHPRINSTLFIWVTSFGMGIFTALAVYRLLKGVSMKKIFFFFYLAVIIIIF
                     FVPSQFQALGFDASGAATGAISVPFILALGVGVSHTVSRNKSAEDSFGIVGIAAIGPI
                     ITVYLYGLIVGNSDTLNVYTPGENSDLLAVMLGNLLPVALAVFPLMLIFLIFQAFFIK
                     LPRRKIGQIIIASLVVFVGLFIFIAATDFGLAFAGKYIGEQFMMSEKLWWLLLPLAFV
                     LGFAITLSEPSVVVFCEQVDELTSGSISKNTIKMALGISVGLATFFAVIKTILDINIL
                     WFIVPFYVITIGLIYFTPTLFVGLAFDGGTVSAGAITSAFLTPVTLGASQALGHTNIL
                     TSGLGILAFISVMPRIVVQILGIIYNYRLKKATPPVYEDISYDATTLSETETEIQEEA
                     TNDA"
     gene            1894..2202
                     /locus_tag="PLU08_02630"
     CDS             1894..2202
                     /locus_tag="PLU08_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70627.1"
                     /translation="MTHKEEWVVMVIITRRKYKAELVKMLYDHEAAMVHQTYAKGSAK
                     ASFLRALGLTQEQNKLFLLGFVKTDQVPAIFSVLEYKYKFKEPNSGFAFTFPLEKISC
                     "
     gene            2215..2523
                     /locus_tag="PLU08_02635"
     CDS             2215..2523
                     /locus_tag="PLU08_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOQ70628.1"
                     /translation="MTESMKALFIIVNAGFSEQIVEVVQNHGARGATIIPARGTGKKF
                     VKVLGIQYEPEREILLSVVEEDIALKITADVKENYGKNTPTNGLCFILPVADYMFKNE
                     "
     gene            2575..6150
                     /locus_tag="PLU08_02640"
     CDS             2575..6150
                     /locus_tag="PLU08_02640"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOQ70629.1"
                     /translation="MGNFIRKHIDTLLLVLNVLVFVLFTLVYPFVTFKHTFLNALFYI
                     LLSAPLITFLVLRFLKYDRKTEESIFYYRRGFNLLALINNYLFMMSAESIFTLIKGHR
                     APLFIIYLIFFALMWLINIFALILELGYLDQLLPLREYATDTAKEETIALAKAAKFKR
                     FEEKVSTMYFFIALVAVLAVIFFIYPLVTEYFTIPLGGDFTQQQIPFYTNGYDDWWRF
                     LKTGEFPLWDSNTFLGVNNIGSNSFYYSMNPFFLPILIFPRELIPQGISILMIGKFVL
                     AAVTMRLYLKYMGVNEKEARFFAVIYAFSGWNTYYLWFNHFMEVAVIFPLIFLGIEKL
                     IKEKVMWVLILSLGLMGFANYFFLMTVGVIGVLYAGFRYFQQLPKVEPSIIGLGIIAF
                     ALGLLLSSVVLFPSLVIALGSDRATDATYLEKLLDLFKNVNSISLKEQRRQIWHLITK
                     WEPYTMEGATPSTQYSYKKFYPLISYYFPVLSNRSVALLNTSNYDNTISSLFSFSPVI
                     ILFIPSLVVSAKKKKVSHFVALAFFLFALFTPFFYHAFHGFTKDYGRWQLIVTFSLIT
                     YVAMSLPEVKAEKPYLLDISIIFNLIMMGITAKVAFSFENKSGFSYMYQREYVIYYQA
                     IMLFISYLVLRLARNTKHEYYLRNAFVFFEIAVMGTITMIGHGFISYKDSVSGGYQNY
                     KDDLTTIREIKALDGSYYRIFNTRAYRGNDNLPMRENYNGLSAFHSLYNFELMPFNKW
                     SKINYNHKGWSLGVFEKRSMLNDFLQVKYYMLNNTNHVIYWSNAAPSVAPYRNVPNTY
                     EFMEELSTDKRFVYKMKEGFTLGFGVDNVISYENRLSDGTYFDLIEKDRALSPIQNEE
                     LYLTSALLSYEDHYEVTRTYSDLVSHDINAFPHQHYTRLSSTNGGLKVKYYEFTGRRY
                     PYEAQTRTLGEVVLPTPQWVSENGNEIISSLVPTELNGAHHVIEYTKKDGTNFVNGVN
                     ELGEQIGGEIIMRLPLSYYNSSTLYYNFRYRVFLYDENYELITFDDHHTLPSSWTDWK
                     TLRSFHTPKAVSKIALVPIGSRVTPPYVHELFTYSQDALESIRQTSITNSLTDVKVTT
                     NTATFKTNYPTRKFVVTTIPYDKGWRVTNENNIKIPVYRVQGGFVGFVSDRGEMSYTM
                     SFRPQYFNMGLVLTFSAGIATLLLELNVYFFKKYKKKKAEENINV"
     gene            complement(6140..6982)
                     /gene="rsmI"
                     /locus_tag="PLU08_02645"
     CDS             complement(6140..6982)
                     /gene="rsmI"
                     /locus_tag="PLU08_02645"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOQ70630.1"
                     /translation="MKRNKSFSGTPFYLVATPIGNLEEFSPRAVNVLRSVDFICAEDT
                     RTSGVLLNHFGIKKELISVHKFNEQSKLELILNKLKNGEKGAFISDAGYPLISDPGHP
                     LVKALIANDFSVSVINGPSALLPALIGSALDTETFTFIGFLKGNNKDVLNTLKRYKSY
                     KDTLIIYEAPHRVVKTIATIYTVFAERKLVIARELSKLHEEYIYTSLAEFSANPPTDL
                     KGEIVLVIEGNKDEENNDLADLLDKVELLISNENMSVKKATSVVALLYNVSKNKLYNA
                     YLKR"
     gene            complement(6969..7802)
                     /gene="ricT"
                     /locus_tag="PLU08_02650"
     CDS             complement(6969..7802)
                     /gene="ricT"
                     /locus_tag="PLU08_02650"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOQ70631.1"
                     /translation="MNDNNERYFISIIYNDGGKSYCFGTNESSLDIGDYVLVESSQGI
                     AVAKVVATPQTMDKYSSPLELKDIVRRANEHEIRQHQDNLKDAELAMAFCQKQVERLG
                     LKMNLLTSEYTFDRSKINITYSAEERVDFRELVKILGSHLKTRVELRQIGSRDRAKLI
                     GGLGQCGFPLCCHTFLTEFDGISINRAKNQMLPLNIPKLSGHCGKLLCCLKYEDDYYT
                     EAKKEFPNVGSTYTRGNTLYQLTSFNIVNRTLRLDYEDGFVIVPLDELKRDYKVKHEK
                     K"
     gene            complement(7795..8781)
                     /locus_tag="PLU08_02655"
     CDS             complement(7795..8781)
                     /locus_tag="PLU08_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70632.1"
                     /translation="MTIYNYLKNYQPILFQTFGNALTTNNLSHAYLLDGEPGTPLKEV
                     AFYLAKSLVCDDPNPFACDKCLACHRFEEGNYTDFYFFDGSESSIKKDSVLELEKAFS
                     MTSVEHKGVLIYVIHNVENMTIEAINSLLKFLEEPAGKVYAFLTTNNLHNVIPTIKSR
                     SQILSLQLIKRDVLLKLCESIALPQDDIEMLVPFYNIPSLIEEEAKSERYPEYKRAVI
                     DTLETFSDNPKRGYLKAHELIVPLRERFVIKRVFTYFALFFKDVVNYNIGHERLFKSY
                     DPLIKKLAATIKNPLPLIVATYEISKKVDDYVNTGLLVDELLIKMVKETTDE"
     gene            complement(8778..9401)
                     /gene="tmk"
                     /locus_tag="PLU08_02660"
     CDS             complement(8778..9401)
                     /gene="tmk"
                     /locus_tag="PLU08_02660"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOQ70633.1"
                     /translation="MFITFEGPEGSGKTSIANRIIKTLIDKGYDIVHTREPGGTPIAE
                     QIRDVTLDKKNTALDARAEALLIAASRRQHVVEKIRPALAEGKIVICDRYLDSSLAYQ
                     GAARGLGIDTVLAINLFAVEDTLPNLTLLFDLEPAIGFERIKANKNREVNRLDLEERN
                     FYIKVRNAYLELSKKYKDRYRIIDASQSFEKVYEDTLKVILEYLPQK"
     gene            complement(9411..10010)
                     /gene="recR"
                     /locus_tag="PLU08_02665"
     CDS             complement(9411..10010)
                     /gene="recR"
                     /locus_tag="PLU08_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOQ70634.1"
                     /translation="MDDLPTLNALIEHLKRLPSVGQKSAERMAQAFLTFDDETLKDFG
                     TVITKLKEKVHLCPNCGLYTEAELCSFCVDPNRNNKTLIVVSYPKDVLGFSKLEDYHG
                     RFHILGGVISPSSNKGVELLSIPELEKRIVRDNVEEIILATNPTVEGELTALFVSKKL
                     ADLPVKITRLAYGLPMGGQVDYADAMTLFKALEGRKDLK"
     gene            complement(10010..10315)
                     /locus_tag="PLU08_02670"
     CDS             complement(10010..10315)
                     /locus_tag="PLU08_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOQ70635.1"
                     /translation="MSSSMQKLMQQAQKMKREMQKAQEALNAKEFSVTKGGGVTVAML
                     GSKEVVEVTIDEDFFDKDNKEMVETLIIMAFNECIEQINAEEAAINEAITGSATGLF"
     gene            complement(10308..12083)
                     /gene="dnaX"
                     /locus_tag="PLU08_02675"
     CDS             complement(10308..12083)
                     /gene="dnaX"
                     /locus_tag="PLU08_02675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOQ70636.1"
                     /translation="MSYKALYRTYRPLTFGEIAGQRAIVRTLQNALNQNKIAHAYLFS
                     GPRGTGKTTMAKLFAKALNCEEGLGHQCNRCTNCNSVNDGSHPDVYEIDAASNNGVED
                     VRQLIENINYAPIRGRYKVYIIDEVHMMTQSAFNALLKTLEEPPQNVIFILATTEPHK
                     VIPTILSRCQRYNFSKVSDRDITKRLKTVLETEVIPYEEEALKLLVSLADGGVRDALS
                     MLDQVLAYSRQQLKLEDVLALFALTSKLEQLNLLRAVASSNVEEVFTILSGLIDKGVD
                     VRRLTTDLLNLLKDALIYYRTENTDLLNYLRKEEVQELNMMFEIENLNMMIENILKAQ
                     ADYRFASDVKNIFEITLLKIITSLNQDTMVEKELIIQKPKVKTAPSVPKKDEKHAIVT
                     ETEKETIVEEQELPPFMTQSREKLEKRVTETKSEIVKDEVKTAQPVPEKVQYANPLHN
                     EGQVIELDDDTIIKILTLADRDEKLSLRNNRWDDLALMAMDSVVGEHANVLLNGAPYA
                     LAPEIILVEFNFDQYARYINILENQKIMQDIIERLVGRRIPVYGLTHGQAVSFARKYR
                     SMSQIQKLPRVKSVKEELEGKFDYV"
     gene            12363..13322
                     /locus_tag="PLU08_02680"
     CDS             12363..13322
                     /locus_tag="PLU08_02680"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ70637.1"
                     /translation="MFNFQLSSNKPQINYDELYDFIALGLGPAGLNAGLYAHRKGLKT
                     LIVGHRVGGQLLNTADIDNYLGFSNIPAQKLIDEFRNHIENSKVPMLTDVLVTNIEKS
                     ADIFIITLNNGKTLQSKTVLYALGGSPRKLKVPGEDRLAGKGVAYCATCDGPFFQDKD
                     VIIAGGGNSAIDATKDLARIAKSVTIIEKFTISADKTSVEQVAKLPNVTMFEETDVTE
                     IIGDKYIEGIRVFDRKANKERIINTEGLFVEIGSIPNTALLKDLVELNKWGEVIVNNK
                     QETSLKGLYAAGDVTNNWIRQVIVAAADGAKAALEANNYITKK"
     gene            13333..13977
                     /locus_tag="PLU08_02685"
     CDS             13333..13977
                     /locus_tag="PLU08_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOQ70638.1"
                     /translation="MGKLFNEEVASQIKGVLAQMKNEITIKLFIDGKCDTCLETKQLL
                     EETKTLSDKINLVVHEINESKEEAEKYDITLTPTFIILDDKGTYSGVRFSGFPGGYEI
                     NSFVSSLLEMSGLRHEFPQEVVDRVAKIKEPIDIKVFVTLGCPHCPGAVETAHRLAML
                     NSNIKATMIESQTFMELARTHKVSSVPKIVFNDALDLLGNQPVEAFLDEIETLL"
     gene            14092..15165
                     /gene="prfA"
                     /locus_tag="PLU08_02690"
     CDS             14092..15165
                     /gene="prfA"
                     /locus_tag="PLU08_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOQ70639.1"
                     /translation="MEDVLRNRLETTKKRLSEIDEELLKPDITSDLDYFRDISVERSQ
                     IEEVVEKYNEYLKLEEDLESSLILMDDKDEEIAEFAKEQHKEIANRLPELINEIRVLL
                     IPKDPYDDKNIVVEIRGAVGGDEANIFAGDLFRMYVKYAERQGWQMQILDENSSAVGG
                     FSQISFMIKGKGVYSKLKYESGGHRVQRVPKTEASGRIHTSIATVLVMPEAEEVDVEI
                     RNEDLEIDTFRSQGAGGQNVNKVESAVRITHKPTGISVACQVEKSQIQNREIAMRLLR
                     ARVLAMKVAEQEEKIGAERKLKVGTGERSEKIRTYNYPQNRVTDHRIGFSTLKLDLVL
                     EGNLEEIIEALVHYDEQQKMVNS"
     gene            15165..15995
                     /gene="prmC"
                     /locus_tag="PLU08_02695"
     CDS             15165..15995
                     /gene="prmC"
                     /locus_tag="PLU08_02695"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOQ70640.1"
                     /translation="MLTLYSLKQNTLKLHNFLDKNDVKLALMHVYNIDSYTDYVLKLH
                     TPIEEKQEFYAIINRLVKGEPIQYILGKAYFYDRYFFVNKNVLIPRVETEELVDIVLK
                     DVKENTGNLLDLGTGSGAIALTLKAHSKLSVFASDISKKALEVAQKNDCENSVTFVHG
                     DLLAPFINKEIAFDYIVANLPYIKREEEVEQKVKDYEPESALYNSDPNIFERLFKEVK
                     MLNFPKTGLSIYLEIGTNQDDEVSNLARRYLGENVKINVVNDMHGKKRFVIIKDIYGN
                     "
     gene            15985..16599
                     /locus_tag="PLU08_02700"
     CDS             15985..16599
                     /locus_tag="PLU08_02700"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOQ70641.1"
                     /translation="METKIFKANEIHLLANIAKEGSIIAFPTETVFGLGVVYDNEEAF
                     NNLVKVKERKTDKPFTLMVGYTRDIENYAVLNTKTKNLIDVFMPGEITLIIEAKPNLP
                     KHVTLGSKYIGIRVSANYEVANLLNLIGKPLLVPSANKRDEKPALTVEEVYKVFNGDI
                     AGILEGETTSHIPSTIVKVDDKIELIREGSIPFNLIKEVWEEEL"
     gene            16596..17039
                     /gene="rpiB"
                     /locus_tag="PLU08_02705"
     CDS             16596..17039
                     /gene="rpiB"
                     /locus_tag="PLU08_02705"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOQ70642.1"
                     /translation="MKVAIGSDHGGFIYKEAVKKHLEDKGITVIDVGSYHEDSVDYPL
                     YGIAVGEKVRDKAADLGIVICTSGEGITIAANKVKGVRAGIGYNDDVSRLIREHNNAN
                     VIGFGGSQMELKDVLRRVDIFLNARFESGGRHSRRVGIITEYENK"
     gene            17080..18249
                     /locus_tag="PLU08_02710"
     CDS             17080..18249
                     /locus_tag="PLU08_02710"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOQ70643.1"
                     /translation="MQDFFICPTCGNNDLNKIGVRNGQKYCRACLPFQGQNAPKITNI
                     PLHIPITLSYELTTEQRRIAEEINANYINKQNSLVYAVCGAGKTELVYLAMQTALARG
                     HQIGFTVPRREVVIELAMRIKKTFPQARVISVFGDHHDALTGNIIVLTTHQLFRYEDY
                     FDLLIFDEIDAFPYVGDLVLESFFKRSVKGVYVLLSATPSTKLLKEFPKVGKVLTLFT
                     RFHNHKIPVPIVKRGFLLFNFMLLLKHLESFIKRKKPVLIFVPTIRLANLIYKLLRID
                     YKNGNVVHSKVKDNSAIIDDFKKGHYAYLVTTTVLERGVTIKGLQVIIYGADHAVYDT
                     ANLVQISGRVGRKKEESDGEVIFIVTKKNAAIKAAISDIEEKNAYLQSVLQRKAT"
     gene            18224..18814
                     /locus_tag="PLU08_02715"
     CDS             18224..18814
                     /locus_tag="PLU08_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOQ70644.1"
                     /translation="MCFKEKQPNFNDLIRDTNVCNKCFRDLDVKFNVSKIAGVTYISV
                     FNYDEHFKTLLFHLKGLKDIVLAPLFLERFIPYFKVRFINYYLVPAPSTIESDKERGF
                     NHVIEIFKPLQRPFLRLITKKVAFKQSDLNYALRQKVKDKLVINNGNKVKGKNILIVD
                     DIKTTGATIMAMINLIKPYNPRKIAILTLAETSLSE"
BASE COUNT         6163 a         3104 c         3410 g         6243 t
ORIGIN      
        1 aaaaaaacga aaacaattta aagtttcttt gtgtgtttta attatcgcta tataaccacc
       61 cccttttacg tcaccaaaaa ggttcgtaaa atattagtat tcactacaaa tgcagtaaat
      121 agggggctaa aagtcttgaa taacaacttt tagaagtctc ttttataata aagagaataa
      181 cgctaaaagt caaatataac cggaattact tttcaatatt aaaatcgtaa agtaatgata
      241 gtacgcatta tttaattatt aatgtaaaaa agttgtaagg agggcagtta taagttaatg
      301 ccatcttaaa atagtcgtaa tatgttatac ttacaaagta taaaaaagag gaagggtgta
      361 aatatatgaa gttcttaaaa aaccttaaag aagcacttat ctcaagttta cccctgcttg
      421 ccgttattat cttagtagcg ggaattattt tccctgtcaa aactaatact gagataattc
      481 aactagtggt aggatatacc ggtgttgtta ttggacaagc gatttttctt actggtctag
      541 attatagtgt cttaccaatt gggaacttga taggtagttc tatcgttaaa ctaaataaat
      601 tagtgttcgt cttgatgttt gcgtttttat ttagtttact cgctgctgtg gcggaaccag
      661 gcattactgt tttagctgca caagttggtg gtattcaccc acgtataaat agcacactct
      721 ttatttgggt aactagtttt ggtatgggaa tatttacggc cttagcggta tatcgtttgt
      781 taaaaggtgt aagtatgaaa aaaatcttct ttttctttta tttagcagtt atcattatta
      841 ttttctttgt tccaagtcaa ttccaagccc ttggctttga cgctagtggg gcggcaacag
      901 gtgcgattag tgttcccttt attttagcgt taggtgtagg tgtttcccat actgttagtc
      961 gcaataaaag tgcagaagat agttttggga ttgttggtat tgcggcaatt ggtccaatta
     1021 ttactgttta cttatatgga ttaatcgttg gtaatagtga cacacttaat gtatatactc
     1081 ccggtgaaaa tagtgattta ctagcggtaa tgcttgggaa tttattgcca gtcgcccttg
     1141 cggtctttcc gcttatgtta atttttctta ttttccaagc attctttatt aagttgccgc
     1201 gaagaaaaat tggtcaaata ataattgcat cacttgttgt ttttgttggg ttatttattt
     1261 ttattgcagc gacagacttt gggttagcgt ttgctggtaa atatattggc gaacaattta
     1321 tgatgagtga aaaactgtgg tggctacttt taccactagc gtttgtactt ggctttgcaa
     1381 ttactttaag cgaaccaagt gttgttgtat tctgtgagca ggttgatgaa ttaactagcg
     1441 gaagcatttc taaaaataca attaaaatgg ctttaggcat ttccgttggc ttagcgacat
     1501 tctttgcagt tattaaaaca attttagata ttaatatctt atggtttatc gtaccttttt
     1561 atgtaattac aattggttta atttacttta caccaacatt attcgttggt ttagcctttg
     1621 atggcggtac ggtaagtgcg ggcgcaatta cgagtgcctt tttaaccccg gtgacactcg
     1681 gggctagtca agctttaggg cacactaata tattaacctc aggcttaggt attttagcct
     1741 ttatcagtgt gatgccgcgt atcgttgtgc aaatacttgg cataatttat aactatcgtt
     1801 tgaagaaagc aacaccacca gtgtatgaag atattagtta tgatgcgaca acgttaagcg
     1861 aaacagaaac agaaatacaa gaggaggcca caaatgacgc ataaagaaga atgggttgtg
     1921 atggttatta ttactcgtcg caaatataaa gcggagctcg taaaaatgct ctatgaccat
     1981 gaagcagcaa tggttcatca aacatatgcg aaggggagcg ctaaagctag tttcttacgg
     2041 gcacttggcc taactcaaga acaaaataag ctctttttgc taggatttgt caagactgat
     2101 caagttccag caatctttag tgtattagag tataaataca aatttaaaga accaaattct
     2161 gggtttgctt ttacattccc acttgaaaag ataagttgct aggaggacat tcttatgact
     2221 gaaagtatga aagctttatt tattatagtt aacgcgggct ttagtgaaca aatcgttgaa
     2281 gtcgtgcaaa atcatggcgc tagaggtgct actattatcc ccgcgcgtgg cactggcaag
     2341 aagtttgtca aagtacttgg tattcaatat gaaccagagc gcgaaatctt acttagtgtc
     2401 gtggaagaag atatcgcgct taagattact gctgatgtga aagaaaacta cggtaaaaac
     2461 acaccaacta atgggttatg ctttattttg cctgttgctg attacatgtt taaaaatgag
     2521 taaataatag tttgataacc attaatcttc aatttatttt ataatgataa tactatggga
     2581 aactttattc gtaaacacat tgatacttta ctcctagttt taaatgtctt agtctttgtt
     2641 ttatttacgc ttgtttaccc attcgtgacc ttcaaacata ccttcttaaa tgcactattc
     2701 tatattttac ttagtgcacc gcttattact ttcttagtgc ttaggttcct taaatatgac
     2761 cgcaagactg aagaaagtat tttttattac aggcgtggct tcaacctttt agcgcttatt
     2821 aataactatt tatttatgat gagtgctgag agtattttta cgcttattaa aggtcataga
     2881 gcgccgttat ttattattta tttaatattc ttcgctttaa tgtggttaat taatatcttt
     2941 gcactaatcc ttgaacttgg ttacttagat caacttttac cgcttcgaga atatgcgact
     3001 gacaccgcga aagaagaaac gatcgcatta gcgaaagctg caaagtttaa acgctttgaa
     3061 gaaaaagttt caacaatgta cttctttatt gcgcttgtgg cagtactcgc ggttattttc
     3121 tttatttatc cactggtgac tgaatacttt acgattcctt taggtggtga ctttacacag
     3181 caacaaatcc ctttttacac gaatgggtat gatgactggt ggcgcttctt aaagaccggt
     3241 gaatttccgc tgtgggatag taataccttt ttaggtgtta ataacattgg ttcaaatagt
     3301 ttttactatt caatgaatcc cttcttctta ccaattctta ttttcccgcg tgaacttatc
     3361 ccacaaggga ttagtatttt aatgattggt aagtttgtac tagcggcagt aacaatgcgt
     3421 ttatatttaa aatatatggg cgttaatgaa aaagaagcac gattctttgc tgtaatctat
     3481 gcatttagtg gctggaatac atattactta tggtttaacc acttcatgga agttgccgtt
     3541 attttcccgc ttattttcct tggtattgaa aaattaatta aagaaaaggt tatgtgggtc
     3601 ctaattttgt cattaggctt aatgggtttt gctaattact tcttcttaat gactgttggg
     3661 gtcattgggg tgctctacgc tggttttcgc tactttcaac aattaccaaa ggttgaacca
     3721 agtattattg gattaggtat tattgctttt gctttaggat tactattaag tagtgtagtg
     3781 ttattcccga gtttagtcat tgctttaggt agtgaccgag cgacagacgc cacttattta
     3841 gaaaaattac tagacttatt taaaaatgtt aatagtatca gtcttaaaga acagcgccgc
     3901 caaatttggc atctaattac taaatgggag ccttacacca tggaaggcgc tactccgagt
     3961 acacagtata gttataaaaa gttttatccg cttatttctt attacttccc agtattaagt
     4021 aataggagtg tcgctctact taatacatca aattacgata acacgatttc aagtttgttt
     4081 tcgtttagcc cagttattat tttatttatc ccttcattag tcgtatccgc gaaaaagaaa
     4141 aaggttagtc actttgtagc acttgcgttt ttcttgtttg ctttatttac gcccttcttc
     4201 tatcacgctt tccacgggtt taccaaggat tatggtagat ggcaactaat tgtaactttt
     4261 agtttaataa cttatgttgc aatgtcgctt cctgaagtga aggcagaaaa gccttatctt
     4321 ttagatataa gtattatctt taacttaatt atgatgggaa taaccgctaa agtagcgttc
     4381 tcttttgaaa ataagagcgg ctttagttat atgtatcaaa gggaatatgt aatttactac
     4441 caagcaataa tgctttttat tagttattta gtccttcgtt tagcgcgtaa cacaaaacac
     4501 gaatattatt tacgcaatgc ttttgtgttt tttgaaatag cggtaatggg gacgataaca
     4561 atgattgggc acggctttat tagctataaa gatagtgtgt caggtggtta ccaaaattat
     4621 aaagatgatt taacaacgat tagagaaatt aaagccttag atggttctta ttaccgaatt
     4681 tttaacactc gtgcctatcg tggtaatgat aatttaccaa tgcgtgagaa ttataacggg
     4741 ttaagtgcct tccatagtct ttataacttt gaactgatgc catttaacaa atggtcaaaa
     4801 attaactata accataaggg ctggagttta ggtgtctttg aaaaacgtag tatgctaaat
     4861 gacttcttac aagttaagta ctatatgctt aataatacga accatgttat atattggagt
     4921 aatgctgcgc caagcgtagc gccttaccgt aatgttccaa acacttatga atttatggaa
     4981 gagttaagca ctgataaacg ctttgtctac aagatgaaag aaggttttac gcttggtttt
     5041 ggagttgata atgttataag ttatgaaaat aggctgagtg acggtactta ttttgactta
     5101 attgaaaaag atcgcgccct ttcccctatt caaaatgaag agctctattt aacaagtgcg
     5161 cttcttagtt acgaagatca ttatgaagta acaaggactt atagtgattt agtgagtcat
     5221 gatattaacg ctttcccgca tcaacattac acgcggctta gtagcacaaa cgggggctta
     5281 aaggtaaagt attacgaatt tactggccgc cgctatccct atgaagcaca aacgagaaca
     5341 ttaggtgaag tcgtgctacc gacaccccaa tgggtcagtg aaaacgggaa cgaaataata
     5401 agtagcttag tgccaacaga attaaatggg gcacatcatg tcattgaata tacgaagaaa
     5461 gatggcacaa attttgtgaa tggtgtaaat gaactaggcg aacaaattgg cggcgaaata
     5521 attatgcgat tacctctttc ttattacaac agcagtacac tttactataa ctttcgttat
     5581 cgggtgtttt tatatgatga aaattatgag ttaattactt ttgatgatca tcatacttta
     5641 ccgagttcat ggacagattg gaaaacttta cggagtttcc acacaccaaa agcggtatct
     5701 aaaattgcgc tagtgccaat tggcagtcgt gttactccgc catatgttca tgaattattt
     5761 acctattcac aagatgcact agaatctata agacaaacat caatcactaa ttcactaact
     5821 gatgttaagg tcacaacgaa taccgcgaca tttaaaacta attatccgac gcgcaagttt
     5881 gttgttacca caattcctta cgataaggga tggcgtgtca ctaatgaaaa caatataaaa
     5941 atacccgttt atcgggtaca aggtggattt gttggttttg ttagcgatcg gggtgaaatg
     6001 agttatacaa tgtcgtttag accgcaatac tttaatatgg gtttagtgct tactttctct
     6061 gcaggtattg ctacattatt actagaacta aatgtgtact tctttaagaa atataagaag
     6121 aaaaaagccg aagaaaatat taacgtttaa gataagcgtt atatagttta tttttagaaa
     6181 cattatagag caaggcgacg acactagtcg cctttttaac actcatattt tcattactaa
     6241 ttaatagttc tactttatct aaaaggtccg ctaagtcatt attttcttca tctttattgc
     6301 cttcgatgac taaaacgatt tcacctttta aatccgtcgg tggattagca ctaaattccg
     6361 ctagtgatgt ataaatatat tcttcatgca atttacttaa ttcacgcgca attacaagct
     6421 ttctttcggc aaaaaccgtg taaatcgtcg ctattgtctt aacaacacga tgcggagctt
     6481 cgtaaattat taatgtgtct ttgtaagatt tataacgctt taaagtgttg agcacatctt
     6541 tattattacc ttttaaaaag ccgatgaatg taaatgtttc agtatctaag gcgctaccaa
     6601 tcagcgctgg taaaagtgca ctaggaccat taataacact aacactaaaa tcgttagcga
     6661 ttaacgcttt aactaacggg tgtcctggat cactaataag cggataaccg gcatcactaa
     6721 taaaggcgcc tttttctccg ttttttaatt tatttaagat taactctaat ttactttgct
     6781 cattaaattt atggacacta ataagttcct ttttaatacc gaaatgattt aaaagaacac
     6841 cgcttgttct ggtatcttca gcgcaaataa aatcaacgct tcttagcaca ttaacagcgc
     6901 gcggcgaaaa ctcttctaaa ttgccaattg gcgtagccac taaataaaat ggtgtaccgc
     6961 taaaagattt atttcttttc atgtttaact ttataatccc tttttaattc atctaagggc
     7021 acaatcacaa aaccatcttc ataatcaaga cgaagagtac ggttaacgat attaaatgat
     7081 gttagttgat acaaagtgtt accgcgagtg taagtcgaac caacattagg gaattctttt
     7141 tttgcttccg tataataatc atcttcatac tttaaacaac aaagtaattt accacagtgg
     7201 ccacttaatt ttggaatgtt aagcggtaac atttggtttt tagcacgatt aatcgaaata
     7261 ccatcaaact ctgttaagaa agtatggcaa caaagcggga agccgcattg cccaagacca
     7321 ccaattagtt tagcgcggtc tctactacca atttggcgta gttctactct agtttttaag
     7381 tgtgaaccaa gaattttcac aagctctcta aagtcaacgc gttcttcagc ggagtaagta
     7441 atattaatct tactacgatc aaaagtatat tccgaagtaa gtaaattcat tttaagacca
     7501 agccgttcga cttgcttttg acaaaaggcc attgcaagtt cagcatcttt tagattatct
     7561 tgatgttgac ggatttcatg ctcattagcg cgtctaacaa tatcctttaa ctctaatggg
     7621 ctactatact tatccattgt ttggggcgtt gccactactt tagcaacggc tataccttga
     7681 ctgctttcta ctaaaacata atcgcctata tctaaagaac tttcatttgt tccaaaacaa
     7741 taagacttgc caccatcgtt atatataata cttataaagt aacgttcatt gttatcattc
     7801 atctgttgtt tcctttacca ttttgattaa taattcatcc acgagtaagc cagtattaac
     7861 ataatcatca acctttttac ttatttcata agtagcaaca attaatggca gtggattctt
     7921 tatcgtcgct gctaactttt taataagcgg atcataactc ttaaaaagtc gctcgtgacc
     7981 tatattatag tttacaacat ctttgaagaa aagggcaaaa tacgtaaata cacgcttaat
     8041 tacaaagcgt tcacgcagcg gaacgattaa ttcgtgggcc tttaagtagc cgcgcttagg
     8101 attgtcgcta aaagtctcta aagtatcaat tacagctctt ttatactctg gatatcgttc
     8161 tgattttgct tcttcttcaa ttaaactagg aatattataa aaaggcacaa gcatttctat
     8221 atcatcttgc ggtaacgcta ttgattcgca aagttttaag agtacatcgc gtttaattag
     8281 ttgtaatgaa agtatttgac ttcgactctt aatagtcggg ataacattat gtaagttatt
     8341 tgtagttaaa aaagcatata cttttcccgc tggctcttcg agaaacttta aaagtgaatt
     8401 aattgcttca atagtcatat tttcaacatt atgaatcaca taaattaaaa cacctttatg
     8461 ttcaacacta gtcatgctga aggctttttc aagttctaat acactatctt ttttaatact
     8521 tgattctgag ccgtcaaaaa aatagaaatc agtgtaatta ccttcttcaa aacgatgaca
     8581 cgctaagcat ttatcacacg cgaaaggatt aggatcatca cacactaaac tttttgctaa
     8641 ataaaaagca acttctttaa gtggtgttcc aggttcacca tcaagcaaat aagcgtgact
     8701 taaattattt gtagttaaag catttccaaa tgtttggaat aaaataggct gataattttt
     8761 taaataatta taaattgtca tttttgtggg agatattcta aaataacttt tagtgtatcc
     8821 tcataaactt tctcaaaact ttggcttgcg tcaattattc gataacggtc tttatatttt
     8881 ttacttagtt caaggtaggc attgcgcacc ttaatgtaaa aattgcgttc ttctaagtct
     8941 aggcgattaa cttcacgatt tttattagct ttaattcgtt cgaaaccaat tgctggctct
     9001 aaatcaaata aaagtgtcaa atttggcaag gtgtcttcaa ctgcaaaaag gttaattgcc
     9061 aggactgtgt cgattcctaa acccctagcc gctccttgat aggcgagcga agaatctaaa
     9121 taacggtcac aaataacaat ttttccctcg gctagtgctg gacgaatttt ctcaacgaca
     9181 tgttgccttc tacttgctgc aattagaagt gcttctgccc gagcatcaag cgccgtgttt
     9241 ttcttgtcta aagttacatc acgaatttgt tcggcaatag gagtgccacc aggttcacgt
     9301 gtatgaacaa tatcataacc tttatcaata agtgttttta taattctatt agcaatacta
     9361 gtttttccgc ttccttctgg tccttcaaat gtaataaaca taacaccctc ctatttaaga
     9421 tcttttcgtc cttctaatgc tttaaataaa gtcatagcat cagcgtaatc tacttgcccg
     9481 cccattggca gtccataagc caatcgtgta atttttacag gtaaatccgc aagtttttta
     9541 ctaacaaaga gtgctgttag ttcaccttct actgtcggat ttgtggcaag aataatttct
     9601 tcaacattgt cacgtacaat tcttttttct aactctggga ttgaaagaag ttcaacgcct
     9661 ttatttgaag aaggactaat aacgcctcct aatatatgga agcgcccatg gtagtcttct
     9721 aatttactaa aacccaaaac atccttagga taactaacga cgattaaagt tttattgtta
     9781 cgatttgggt caacgcaaaa agaacataat tccgcttctg tgtaaagacc acaattagga
     9841 cacaggtgca ccttttcttt taatttagtg ataactgtgc cgaaatcttt taatgtctca
     9901 tcatcaaatg ttaaaaaagc ttgtgccata cgctcagcac ttttttgacc aacacttggt
     9961 aaacgtttca agtgttcaat taatgcgttt agtgttggta aatcatccat taaaatagtc
    10021 ctgttgcact tcctgtgatt gcttcattaa tagcggcttc ttctgcattt atttgttcaa
    10081 tacattcgtt aaaagccata atgataagcg tttcaaccat ttctttatta tctttatcaa
    10141 aaaaatcttc gtcaattgtc acttcgacaa cttctttact tccaagcata gcaacagtta
    10201 cacccccacc tttagtaacg gagaactcct ttgcgtttaa tgcttcttgt gctttttgca
    10261 tttcgcgctt cattttttgc gcttgttgca ttaatttttg catactacta gacataatca
    10321 aatttccctt ctaattcttc ttttacgctc ttaacacgcg gtaatttttg aatttggctc
    10381 atcgagcgat attttcttgc aaaactcaca gcttgaccat gggtaagccc atagactggt
    10441 atgcgacgcc ccactaacct ttctataata tcttgcataa ttttttgatt ttctaaaata
    10501 ttaatataac gagcatactg atcaaaatta aattcgacga gaatgatttc tggggctagg
    10561 gcataaggag caccattaag caatacatta gcgtgttcac caactacact atccatagcc
    10621 atcagcgcta aatcatccca acgattattt ctaagtgaca acttttcatc gcggtcagct
    10681 agtgtaagta ttttgataat agtgtcatcg tctaattcaa taacctgacc ctcattatgt
    10741 aaaggatttg catattggac cttttcaggc acaggctggg cagttttcac ttcgtcctta
    10801 actatttctg atttagtttc ggtgacgcgc ttttctagtt tttcccttga ctgtgtcata
    10861 aaaggtggta attcttgctc ttcaactata gtttctttct cagtttcagt aactatagcg
    10921 tgtttttcat cctttttcgg cacagatggg gcagttttta cttttggttt ctgaataatt
    10981 aactcttttt cgaccattgt gtcttgattt aatgatgtga taatttttaa aagtgtaatt
    11041 tcaaagatat ttttgacatc acttgcaaac cgataatctg cttgagcctt caatatgttt
    11101 tcaatcatca tgtttaagtt ttcaatctca aacatcatat ttaattcttg cacttcttct
    11161 ttgcgcaagt aatttaataa atcagtattt tctgtccgat aataaataag tgcatctttt
    11221 aataaattaa gtaaatctgt tgttaaacgt cttacatcaa cacctttatc aattaaacct
    11281 gatagtattg taaaaacttc ttcgacatta ctactagcaa ctgctcttaa taagttaagt
    11341 tgttctagtt tactagttag ggcgaaaagc gctaatacat cttcaagttt taattgttga
    11401 cgagagtaag ctaaaacttg gtctaacatg cttaaagcat cacggacgcc tccgtctgcc
    11461 aacgacacta ataattttag tgcttcttct tcataaggaa taacttcagt ttctaaaact
    11521 gtttttaaac gtttagtaat gtcgcgatca cttacttttg agaagttata gcgttgacag
    11581 cgacttaaaa tcgttggaat taccttgtgt ggctcagttg ttgccagaat aaaaataaca
    11641 ttttgtggtg gctcttcaag tgttttaagc aaagcattga atgcactttg tgtcatcata
    11701 tgaacttcgt caatgatata tactttataa cgacctctaa tcggcgcata atttatattt
    11761 tcaataagtt gacgaacatc ctcaacgcca ttattactgg cggcgtcaat ttcatataca
    11821 tcaggatggc tgccatcgtt aacactatta caattagtgc aacgattaca ttgatgacca
    11881 agaccctctt cacaattaag ggctttagca aagagtttag ccatcgttgt tttaccagta
    11941 ccgcgcggtc cactaaaaag ataagcatgt gcaatcttat tttgatttag ggcattttgt
    12001 aaagtgcgga caattgctct ttgtccagct atttcgccaa aagtaagtgg ccgatatgtt
    12061 cgatatagtg ctttataact catgtagtaa ttatacaata attaagacta cattgtagga
    12121 ataagaacac aaaacttaaa ttgttttgat aatatgcagg atgatgactt tttattgctt
    12181 cataatgtgt ttaattctgc aattagttta atgtttcttt atattaataa ttgttataaa
    12241 ttttattgtc taaaaacata aaaacacgca ttttatgtag cgacactttt tgcattaagc
    12301 aaattgtttt tatttaagtt aatcgaccta tataatatcg gcagaaagga gaaaactata
    12361 ttatgttcaa tttccaacta agctcaaata aacctcaaat taattatgat gaactctatg
    12421 attttattgc tcttggatta ggtccagctg gtctaaatgc tggattatac gctcatcgca
    12481 agggcttaaa aactctaata gttggtcatc gtgttggcgg tcaattatta aatactgctg
    12541 atattgataa ttatctaggt ttctcaaata ttcccgcaca aaaattaatt gatgaattta
    12601 gaaatcatat tgaaaattca aaagtaccaa tgctcactga tgtgttagtt actaacattg
    12661 aaaaaagtgc tgatatattc attattacac tcaataacgg caagacttta caaagtaaaa
    12721 ccgtgttata tgctcttggg ggtagtccgc gcaagctaaa agtgcctggt gaagatagac
    12781 ttgctggtaa aggagtcgct tactgcgcta cgtgtgacgg accattcttt caagataagg
    12841 atgtaataat cgcaggtggg ggtaatagtg cgatagacgc cacgaaagac ttagcgcgta
    12901 ttgcaaaaag cgtgacaatt attgaaaaat tcacgataag cgctgataaa actagtgtag
    12961 aacaagtggc aaaattacca aatgtaacga tgtttgaaga aactgatgtt acagaaatca
    13021 ttggtgataa atatattgaa ggaatcagag tgttcgacag aaaggcaaat aaagaaagaa
    13081 ttattaacac tgaaggctta tttgtcgaaa ttgggagtat tccaaatacc gctctattaa
    13141 aagaccttgt tgaattaaac aagtggggcg aagtaattgt taataacaaa caagaaacaa
    13201 gtcttaaagg tctatatgca gcaggagatg taactaataa ctggattcgt caagtcatcg
    13261 ttgctgcagc cgatggagcc aaagctgctt tagaagcaaa caactatatt acaaaaaagt
    13321 aaaggagaaa ttatggggaa attatttaac gaagaagttg caagccagat taaaggcgtg
    13381 cttgcacaaa tgaaaaatga aattacaatc aaattattta ttgatggcaa atgtgacact
    13441 tgtttagaaa caaaacaact attagaagaa actaaaacat taagcgacaa aatcaatctt
    13501 gttgttcacg aaattaacga atcaaaagaa gaagcggaaa aatatgatat tacgcttact
    13561 ccaacattta taattctcga tgataaagga acatatagcg gagttagatt cagtggtttt
    13621 ccaggaggtt atgaaattaa ttcattcgtt agttcacttt tagaaatgtc tggattacgc
    13681 cacgaattcc cacaagaagt agtggaccgt gttgctaaaa ttaaagagcc aattgacatt
    13741 aaagtattcg tgactctagg ttgtccgcac tgtccaggtg ctgttgaaac cgctcatcgt
    13801 ttagcaatgt taaatagtaa cattaaagca acaatgattg aatcacaaac atttatggag
    13861 ttagcgcgta cacataaagt ttctagcgtt ccaaaaattg tgttcaatga tgcacttgac
    13921 ttacttggta atcaaccagt ggaagctttc ttagacgaaa tcgaaacttt attatagaaa
    13981 cacttacatt aaggctccta ctactattag gggccttttt cttatataaa taagaagata
    14041 taatgaaaaa gcattaaaaa tataatataa tacgaccgca aggataaata tatggaagat
    14101 gtattaagaa atagattaga aacaacaaaa aagcgtttat cagagattga tgaagagtta
    14161 cttaaaccgg atattacaag tgaccttgat tattttcgtg atatttccgt tgaacgctca
    14221 caaatagaag aagttgtgga aaagtacaac gaatatttaa aattagaaga agatttagaa
    14281 tcttccttaa tcttaatgga tgataaagac gaagaaatag cagaatttgc taaagaacaa
    14341 cataaggaaa tcgctaacag attaccagaa ttaatcaatg agattagggt gcttttaatt
    14401 ccaaaagatc cttatgatga caaaaacatt gttgttgaaa ttagaggcgc tgttggggga
    14461 gacgaggcaa atatttttgc gggtgatcta tttagaatgt atgttaaata tgctgaaaga
    14521 caaggttggc aaatgcaaat ccttgatgaa aatagtagcg ccgttggtgg cttttcgcaa
    14581 atctccttta tgattaaagg taaaggtgtt tattcaaaac ttaaatatga aagtggtggg
    14641 catcgtgtgc agcgtgtacc aaaaactgaa gcaagcggaa gaatccatac ctcaatcgct
    14701 actgtgttag tgatgcctga agcagaagaa gttgatgttg aaattcgtaa tgaagattta
    14761 gaaattgata cttttagaag tcaaggcgct ggtggacaaa atgttaacaa agttgaatcc
    14821 gctgtacgta ttacgcataa accaactggt atcagtgttg cttgtcaagt ggagaaaagc
    14881 caaattcaaa atcgtgaaat agcaatgcgt ttattacgcg cccgtgtctt agcgatgaaa
    14941 gttgctgaac aagaggaaaa aatcggtgct gaacgtaaac ttaaagtcgg cactggcgaa
    15001 cgaagtgaaa agattagaac ttataactat ccgcaaaata gagttactga tcaccgtatt
    15061 ggctttagca cattaaaatt agatttagtg cttgaaggaa atttagaaga aattattgaa
    15121 gcgctcgtgc attatgatga acaacaaaaa atggtaaata gttaatgtta actttatact
    15181 ctttaaaaca aaatacatta aaactacata attttctcga caaaaatgat gtaaaattag
    15241 cattaatgca tgtttataat attgattcgt atacagatta tgttttaaaa ttgcatactc
    15301 ccattgaaga aaagcaagag ttttatgcaa ttattaatcg tcttgttaaa ggcgaaccta
    15361 ttcaatatat tttaggtaaa gcttattttt atgatcggta ttttttcgta aataaaaatg
    15421 tgttaatacc gcgtgttgaa actgaagaat tagtggatat tgtgcttaaa gatgtaaaag
    15481 aaaatacagg taatttactt gatttaggta caggaagcgg tgcgattgca ttaactttaa
    15541 aagctcattc caaattaagt gttttcgcta gtgatataag taaaaaagca ttagaagtcg
    15601 cccaaaaaaa cgattgtgaa aatagtgtaa cttttgttca tggcgactta ttagcgccat
    15661 ttattaataa agaaatcgct tttgattata ttgttgcgaa tttaccttat ataaagcgcg
    15721 aagaggaagt tgaacaaaaa gtaaaagatt atgaacccga aagcgcactt tataatagtg
    15781 atccaaatat ttttgaaaga ctatttaaag aagttaaaat gttaaacttt cccaaaacag
    15841 gattatcaat ttatttagag attggcacta atcaagacga cgaagtaagt aatttagcgc
    15901 gccgctactt aggagaaaat gttaaaatta atgttgtgaa tgatatgcac ggtaaaaagc
    15961 gctttgtcat aattaaggat atttatggaa actaaaatat ttaaagcaaa tgaaattcat
    16021 ttattagcaa acattgcgaa agaagggagt attattgctt ttccgactga gacagtattt
    16081 ggtcttggtg ttgtttatga taatgaggaa gcctttaaca atttagtgaa agttaaagaa
    16141 cgtaaaactg ataaaccgtt taccttaatg gttggttata cacgagatat tgaaaattat
    16201 gcggttttaa acactaaaac taaaaattta attgatgtat ttatgcctgg agaaataact
    16261 ttaattattg aagcaaaacc taatttaccg aaacacgtca ccctaggttc taaatatatt
    16321 ggcataagag taagcgctaa ttatgaagtt gctaatcttt taaatttaat tgggaaacca
    16381 ctacttgtac ctagcgctaa taagcgggat gaaaaacctg ctttaacagt tgaagaagta
    16441 tataaagtct ttaatggtga tattgccggc attcttgaag gcgagacgac atcgcatatt
    16501 ccgtccacaa tcgttaaggt ggatgataaa atagaattaa ttagagaagg aagcattccc
    16561 tttaatttaa ttaaagaagt ttgggaggaa gaactatgaa agtagcaatt ggcagtgatc
    16621 atggtggctt tatttataaa gaagcagtaa aaaaacattt agaagataaa gggattaccg
    16681 taattgatgt cggttcatat catgaagata gtgtagatta tccgctttat gggatagctg
    16741 ttggtgaaaa agttcgtgat aaagctgctg atttaggaat agttatttgt acatcaggcg
    16801 aaggcattac tattgccgca aataaggtta aaggggtacg cgctggtatt ggttataatg
    16861 atgatgtttc gcgtctcatt cgtgaacata ataatgccaa tgtaattggt tttggtggtt
    16921 cacaaatgga attaaaggat gttttacgcc gtgtcgatat ttttcttaat gctagatttg
    16981 aaagtggtgg tcggcattcg cggcgtgtcg gtatcatcac tgagtacgaa aataaataat
    17041 tactgataaa taaagggtct tgcttacata cttataaata tgcaagactt ttttatttgc
    17101 ccgacttgcg ggaataacga tcttaataaa ataggagtaa gaaacggcca aaaatattgc
    17161 cgagcatgtt tacccttcca agggcaaaac gcgcccaaaa taactaatat tccactgcat
    17221 ataccaataa cactaagtta tgaattaaca acagaacaaa ggcgaattgc cgaagaaatc
    17281 aatgcaaatt acataaataa acaaaattca ttagtgtacg ctgtatgtgg cgctggtaaa
    17341 actgaattag tgtatttagc aatgcaaact gcactagcgc gtggccatca aataggtttt
    17401 acagttccgc ggcgtgaagt cgtaattgag ttagcgatgc gcattaaaaa aacttttccg
    17461 caagcgagag taatcagtgt ttttggtgac catcacgatg ctttaactgg caatattatt
    17521 gtacttacta ctcatcaact ttttagatat gaagattatt ttgatttatt aatttttgat
    17581 gaaattgatg cttttcctta tgttggcgac ttagtgcttg aatcattttt taaacgcagt
    17641 gtcaagggtg tatatgtttt actaagtgct actccgagta caaagctatt aaaagagttt
    17701 ccaaaagttg gtaaagtgtt aactcttttt acaagatttc ataatcataa aatcccagtg
    17761 ccaatagtga aacgcgggtt tttactattt aattttatgt tgttattaaa acatttagaa
    17821 tctttcatta aaagaaagaa accagtttta atttttgtac caacgataag actcgctaat
    17881 ttaatataca aactattacg catcgattat aaaaatggta atgtagtgca ttcaaaagta
    17941 aaagataatt cagcgataat tgatgatttc aaaaaaggac attacgccta cttagtaacg
    18001 acaacagtct tagaaagagg ggtaacgatt aaagggttac aagtaataat ttatggcgca
    18061 gatcacgcag tgtacgacac tgctaattta gtgcaaattt ctggaagagt cggacgaaag
    18121 aaggaggaga gtgatggtga agttattttc atcgttacga aaaagaatgc ggcaatcaaa
    18181 gcagcaatta gtgatattga agaaaaaaac gcctatttgc aaagtgtgct tcaaagaaaa
    18241 gcaacctaac tttaatgatt tgattcgtga cactaatgtt tgtaataaat gttttcgtga
    18301 tttagatgtt aagtttaatg tttcaaagat tgcaggagtt acttatatta gtgtttttaa
    18361 ttatgacgaa cattttaaaa cactactttt ccatctaaaa ggtttaaaag acattgtgct
    18421 agcgccactt tttcttgaaa ggtttattcc ttactttaaa gtacggttta ttaattacta
    18481 tttagtgcca gcgccttcaa caatagagag tgataaagaa cgcggattta atcatgtcat
    18541 cgaaatattt aaaccgctac aaagaccttt tttaaggctt attacaaaga aagttgcctt
    18601 caaacaaagt gacttaaact atgcactccg gcaaaaagta aaagataaat tagtaattaa
    18661 taacggaaat aaagtaaaag gtaaaaatat tttaatcgtt gatgatatta aaacaacagg
    18721 tgccacaatt atggcaatga ttaacttaat aaaaccctat aatccgcgga aaattgcaat
    18781 attaacacta gcggaaacat cgctaagcga gtaactatca tatttttata gaaaatatgg
    18841 taaactttta tagatacatg atacgaagga gattaactat tcttaatcaa gttaaatagt
    18901 aaatatatat caaaaagatt
//
LOCUS       DAOSTA010000017        18901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_3067_length_18901_cov_9.974530, whole genome shotgun sequence.
ACCESSION   DAOSTA010000017 DAOSTA010000000
VERSION     DAOSTA010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 18901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18901
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3067_length_18901_cov_9.974530"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..2125
                     /locus_tag="PLU08_02720"
     CDS             383..2125
                     /locus_tag="PLU08_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HOQ70645.1"
                     /translation="MINRKLLKLIDNHKHIIFTVILMVCGLITNIVITGSLVYLIKEV
                     ANHSDDSSVWTYLWPALIAIISIIIRYLLSISVGYLTDIIGRQAKKDLRQKVYNKIVR
                     LGVRTTDGMKVAGLTQVSLEGVEQLDLYYSTYIPQFFYALLAPIILFFVTVWFDWRFS
                     LVLLAAVPLIPLSIIAISKYAKRIFAKYWGKYTAMGDKFLDSIQGLRELKIFKADAYQ
                     QTKMNEASEEFRVITMKVLVMQLASTTIMDIVAYTGAGIGVLIAIISVLKWGLSPFSA
                     LFLILVAVEFFLPLRALGSAFHVAMNGASAGQKILSLLAEEETPWGTKEVTNANIEIK
                     DVTFAYNGHANVLNDVSMTFKEQGMVSIVGESGSGKSTIVNLITGALRPQFGSVSVGG
                     ELLETLSRESYYSNLAVISYNTYIFNDTIRNNFKLANKDVTDEEIYAALTKVNLADFV
                     EANGGLDKVINEDALNISGGERQRLAVAINLVTNKAIYIFDEATNNIDIESEAIIMAN
                     IKELSKTKAVIVISHRLANVVPSDYIYYMENGELLESGNHDTLMANNSGYAKLFTTQK
                     ALEEGYMECNYEKA"
     gene            2112..3806
                     /locus_tag="PLU08_02725"
     CDS             2112..3806
                     /locus_tag="PLU08_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ70646.1"
                     /translation="MKRPNVIRRSGAKIMASLVVLLGSLSYIMVLAIITGSLGFIFAM
                     GVTLLGAVAIAKFLGEAIALSYPLIFSLLIGFGVLRGILRYIEQYTNHYIAFRLLAVL
                     RDKIFSALRTLFPSKIESKQKGSIIAMITSDIEILEVFYAHTISPIFIAIVVSISVIL
                     FVGFSSSWLLAVNALISYIVIGILLPLITSKKLKVPGVRYRQNFAAFNSFFLDSIKGI
                     KDIVLNNGGPKREVEVNERSDMLLNETKKLKKDTTRAHATTELFVSIFIFITLVLGAI
                     LVIRDNLTIGRMIIGVAAIFGSFGPVIAISSLPSNLTQTFAAGDRVLDLLNEKPLITK
                     IDNGSDFEFENVTVSNVSFAYAENEEILNDVSVHANKGEIVGIVGPSGCGKSTLLKLL
                     LRFYKKDEGTILYNGIAIENINSDNLLDNVTMVSQDTYLFDDTILENLLIAKRDATLK
                     EVEIACKKASIYDFIVSLPQGFNSRVGVFGDNISAGEKQRIGLARAFLRGSKLILLDE
                     PTSNVDSINEGIILRALKKHKEDRGIILVSHRESTMAIADRVYRFNGEIVEEVEDK"
     gene            3807..4253
                     /locus_tag="PLU08_02730"
     CDS             3807..4253
                     /locus_tag="PLU08_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOQ70647.1"
                     /translation="MKAKKIFWIILGCVSLALGAIGAALPILPTVPFLLLAAFSFAKS
                     SKRLHNWFINTKLYKKNLASYVKGKGMTWPAKIKMMITVTLVMLLGFIPMMIKKVYIP
                     SIILGVVWIFHIIWFIFGIKTYKKNSEDSLSTEVETPSETEEKNIE"
     gene            4372..5094
                     /locus_tag="PLU08_02735"
     CDS             4372..5094
                     /locus_tag="PLU08_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOQ70648.1"
                     /translation="MKVENIYKQKYVLTTNYYDPSNNIGVQGTLDLFQNIAARHAYLL
                     GAGREHLEKINLVWVIARTEIEYVTYPETPLEVEVVTWPRPATRFYFDRYYKIVNPAT
                     NEIIAYSRSRWVLIDIDSRKIVGADRYDYPLDFYYEEEPFNHTFPRPSVSDNVVGNYT
                     VRPSDIDENGHLNNSKYGNIIYDYLDLQKGQKIKSIVINYNNEALQGETLTISRSRDD
                     AKVVIFGHKGDTIVFSSEVNIQ"
     gene            5094..5915
                     /locus_tag="PLU08_02740"
     CDS             5094..5915
                     /locus_tag="PLU08_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOQ70649.1"
                     /translation="MLTTLELWIIIIGMAIPFIATALGAAAVFFFRGPISERVNSILL
                     GFAAGVMIAASVWSLIMPSLEFAENSGWGKLAIIPVAVGFALGVLFLWFIDRIVPHMH
                     QRSEDEEGLPTKKLSLDSKLFLAMTIHNFPEGIAVGFAFAVAYTGIVNPGNASISLYS
                     ALALSIGMAIQNIPEGAAVSLPMARRLNNKGKAFGFGVASAIVEPIGAVLAFLFATVF
                     QTLLPWFLSFAAGAMMFVVVEDLIPEANLSGTEHQGTFAVMLGFIIMMIIDIVLG"
     gene            5921..5997
                     /locus_tag="PLU08_02745"
     tRNA            5921..5997
                     /locus_tag="PLU08_02745"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5955..5957,aa:Arg,seq:ccg)
     gene            complement(6055..6864)
                     /locus_tag="PLU08_02750"
     CDS             complement(6055..6864)
                     /locus_tag="PLU08_02750"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOQ70650.1"
                     /translation="MNKPKDIKIIILLGQSNMEGHSWTSYLDRHFPPELVNYYTSPFK
                     TEIAYNCDNGNNESYDEFVPVRVGQGYDSTRFGPEIGIAAHLEKAQHSDEFKIIKFAI
                     GGTSLEVFWRSPSSGIPGLCYVFATNYAHRMLTKLKRDGYNPTVSAILYMQGENDSVD
                     GGHLQYEKYLQNLVNDLRNEFKDYAPKAGIYFIDGLINDAEFWTHHEIINTAKRNVAK
                     SDPKNIIVDTLTPQLSFSNEPDFEIDWAHYDSDGTIKLGHLFGEAVTEHIK"
     gene            complement(6870..7397)
                     /locus_tag="PLU08_02755"
     CDS             complement(6870..7397)
                     /locus_tag="PLU08_02755"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOQ70651.1"
                     /translation="MAKKRLLIVVDYQNDFVNGALGFSDAHEIEQKIIDTIEDHLKTK
                     DDIVFTKDSHFDNYLETEEGRNLPFKHCIKGTPGHDIHGKVAAYLPKAKKVYEKLTFG
                     SLELANDIVNKGYEEITLVGLVTNICILSNALLAKAALPEAKVIVLKDGVNSYDKDLH
                     AKTLDVLQGTQIIVK"
     gene            complement(7414..7506)
                     /locus_tag="PLU08_02760"
     tRNA            complement(7414..7506)
                     /locus_tag="PLU08_02760"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7468..7470),aa:Ser,seq:cga)
     gene            7605..9353
                     /locus_tag="PLU08_02765"
     CDS             7605..9353
                     /locus_tag="PLU08_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70652.1"
                     /translation="MQDIINFILENLPRVILLSGTIVMLVIAVLLIIHGNRKDLKDQN
                     IILGNITTPRIFLLDLGEGKVTYFNRGSFGERKNGLLPQFFQQFTAKEVDRLDKWVNA
                     LIDPKSEASKFYKVDVFVRDMRRSYASILEVTGVDYEKKIIHLESYLYRYLKANDQMN
                     LTVKKKENIGYLTEEELVKVYRRAHKRGRGVYGAISIAVTASGAQTHRAEIPPHLWYE
                     LQNQVAVYRKRSRVIRFVDNHTVGIYFPRSSDVDTAIDVFQSIKKRMSGYLQKNGYNE
                     YSLGIGLTRSRQFSEMNMFFKTAEELSLYALKEDGRLLIHDPNKEIKDMDLSLYRSEL
                     TQLVARKGVDTLFQPIVNAKTVEILGYLTTFSISGSLFGSFNEAAQFARENKQNEQLV
                     KMVLRKAAARYYNMRRNNKQLLFVPLGLGGVDFISDAFSTQPFANDLKMVFMLEEQDF
                     ANTFVNIEDMLKLIKKIKKQKYEVCLIISDTELTLPDKIYGEVDYFMVNEKILTHTVN
                     DERERLSLLSSLGKLLRHQKPIMISDLPKWSEIEYYIRAGVDYVASDEISKKDNSLQT
                     IDKKKALRIINFSKRK"
     gene            9356..10789
                     /gene="proS"
                     /locus_tag="PLU08_02770"
     CDS             9356..10789
                     /gene="proS"
                     /locus_tag="PLU08_02770"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOQ70653.1"
                     /translation="MSKIKIDAITTQSEDFAQWYTDVCRKAELMDYSEVKGFIIYRPY
                     GYAIWENIQKYLDEKFKEKGHDNVYMPLVIPTSLFQKEKDHIEGFAPETLVATQGGNQ
                     KIEDPLIIRPTSEVLFCDHYAKIVSSYRDLPKKYNQWCSVVRWEKTTRPFLRGSEFLW
                     QEGHTIHETEVEARTHALDMLKVYENMGKELLAIPFLTGTKTEKEKFAGAEQTFTIEA
                     LMPDGKALQCGTTHYFGDGFARVFNIQFQDRNNTLQYVHQTSFGVSTRLIGSVIMAHG
                     DDNGLSLPPRLAPVQVIIVPIQQNKPGIMPKALELVSRLKAIGVRVKIDDSDKSPGWK
                     FSEHEMKGIPLRLEIGPRDLKAEVLSGSIRHSHEKISFADKDLEEVVPALLEKIHEDM
                     YQKKLTDLLNHTIEVRSIEELEDALNNKLAYAKMMWSGDQEDLDVIKEKYQATARALP
                     FNQTPFAKKCLISGKPAKYVVIIARAY"
     gene            10833..10923
                     /locus_tag="PLU08_02775"
     tRNA            10833..10923
                     /locus_tag="PLU08_02775"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10867..10869,aa:Ser,seq:gct)
     gene            11204..11773
                     /locus_tag="PLU08_02780"
     CDS             11204..11773
                     /locus_tag="PLU08_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70654.1"
                     /translation="MILNELFKQHSILLLLFAVLVWTIFKFPKIVKRQKANTNNSRMY
                     FAKKHYNHSGKTRKQVLGKDNDLKVNELPLFNHPNKKECFVITKAMHDRIGKHKFIPL
                     YKSGDNIAGVILTSKSSYGKKKNFQIKGGYLGHELHHCIAKINLTKKDLRYSNNISYD
                     ESNYLQFLKANKKNLEKYNEFINKKQLSK"
     gene            12186..12932
                     /locus_tag="PLU08_02785"
     CDS             12186..12932
                     /locus_tag="PLU08_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70655.1"
                     /translation="MFLQNLLETLIKLDVKTMMGVLICGNLVLSLLTLRYYIYHDSNY
                     DKTIIQRFGIAKILQAIAWLFLFFRGEISDVISVYFGNILLFVSFYLDSLIVLRLNDK
                     WTKGWVITQSVLLAVAVILFLVFEITLSAAHIRVGIASLAVFLVFAAPNVLSILNKES
                     TKFQKVIAAISLLFLIFLLLRAFNSLFDNEIVLFTNNFFQIGTFLALVLLMLTNGVGF
                     LLIMYDRSYALVKESSNLDPLTRIYNRRFL"
     gene            12929..13375
                     /locus_tag="PLU08_02790"
     CDS             12929..13375
                     /locus_tag="PLU08_02790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOQ70656.1"
                     /translation="MNKAESYFKRAQQEDGYLSFLFIDIDFFKKVNDTYGHLFGDEVL
                     KVVAKTIAENIRPLDLCCRFGGEEFLIYLHDTSKEEAITIGNRIREVVQLLEFKQVKE
                     YKCTISVGVYSAKPNTETNLQQFIDNVDQALYKAKASGRNCVMSFD"
     gene            13398..14279
                     /locus_tag="PLU08_02795"
     CDS             13398..14279
                     /locus_tag="PLU08_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOQ70657.1"
                     /translation="MKTAMMTGHVTRFGDLEFALRLIKELGFEAVDLTMYDTFGGVAV
                     RNRELFTGDIIANAKRIKSIAEEINLPIVQAHTPFHVYRKDDEEYNAYMLETQKLCLQ
                     IAGIIGIPYVVIHPVMNADNEFNKKFFDELLPLAKKMNVVIATENMWEWQVGAIHCDY
                     STCSTPESFLSLLEYINHPNLKACVDVGHAQMFNGFDKSVSPKLMIEKLAHKYCVALH
                     LHDNDCVNDYHKIPFDGVINWEEVYDALTKINYQGHLVTETSLPREFNLEESKEFWLK
                     QREAADKIKSELLKRRA"
     gene            14492..15265
                     /locus_tag="PLU08_02800"
     CDS             14492..15265
                     /locus_tag="PLU08_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HOQ70658.1"
                     /translation="MFLILSEMLSELQIQVIQLLVVFVFIVTLDVLLLITLKRIFPKV
                     TLIVLSVVLLVLGLFDLTYAFYLVALIYMLCAMTFIFGNIVEFRWLISNAATPKDRRF
                     QRDFRKIYDQEDLVNQLVSAVFSLSKTKTGALITIERTNQLTELIKNGSPIDAPVSAP
                     LLTTIFYKGTALHDGAVVIRDDTILAASVYFTPSTKALAGKVGSRHRAALGISEISDA
                     VTIVVSEETGRISIAYRAALQPVSPEDFRKVLVDHLLTK"
     gene            15286..16599
                     /gene="glmM"
                     /locus_tag="PLU08_02805"
     CDS             15286..16599
                     /gene="glmM"
                     /locus_tag="PLU08_02805"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOQ70659.1"
                     /translation="MKLFGTDGIRGPANVLLTPGLSFDIGAFLGQYQKKNKILIAYDT
                     RRSGIVIFASLVAGILSSGGEASSIGVAPTPALAYLCKKYNYDYGIMISASHNPFQDN
                     GIKIFDTNGFKIAESIEEEIEKYIASDTRLKLKTGGEIGKFTDKSELIYEYVDYAKKA
                     FRGGLKLNLLIDGANGSASSVIHHIVAGTSLNATIINVEPDGVNINADCGSTHLDKLQ
                     DTIKKSPGKYDLGVAFDGDADRVLFVGPKGEVLDGDHVLYLLGKHYKKIGTLTKDTVV
                     ATVMANYGLGSALSKLGIKLVATSVGDKHVQREMIEHDYLIGGEQSGHTIFNGYIKTG
                     DGIYTMMKVLDVLAAEKTTFSEYVKEFIKFPQCLLNIRVKDKKMAMTDKTLLAEIKKS
                     EAKLGGHGRILVRASGTEELVRVMVEAETEAEAKATAEHLASFVK"
     gene            16670..16936
                     /locus_tag="PLU08_02810"
     CDS             16670..16936
                     /locus_tag="PLU08_02810"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70660.1"
                     /translation="MKGGMSMELNEKIIARLIEPADIAFLNQKQPFDLAVLYGELDEE
                     SQAYLLSLLTDESRADLLSFLNPEDAAEFLEEMEIEEQQNILSY"
     gene            complement(16938..17165)
                     /locus_tag="PLU08_02815"
     CDS             complement(16938..17165)
                     /locus_tag="PLU08_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70661.1"
                     /translation="MSITSLIGVVERAAIKSLMVVAPTYLLFSSWKKITSISSLSAAL
                     AIVLYVAFLTVVVFGKEIKCGELTAAALSAS"
     gene            17179..17394
                     /locus_tag="PLU08_02820"
     CDS             17179..17394
                     /locus_tag="PLU08_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOQ70662.1"
                     /translation="MYEDEKFTRALKMIRDYDILMLPVLTRDGEMIGFVSETDALNLM
                     AESHETMMRQLVKVAEDDDNLPPHKTP"
     gene            17484..17909
                     /locus_tag="PLU08_02825"
     CDS             17484..17909
                     /locus_tag="PLU08_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOQ70663.1"
                     /translation="MGLVLFQPMILGMAGNIGTQSMGVTILSLHSGEIKIRKHFGREL
                     LIGLLNSFASGIVGFGIVFLFLTILGQDNALMYAAVVGLSLMCAMIISSLAGVLTPII
                     LLAVKLDEKAASGPLITTINDFFALQIYFLIATLLFTVL"
     gene            complement(17977..18795)
                     /locus_tag="PLU08_02830"
     CDS             complement(17977..18795)
                     /locus_tag="PLU08_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70664.1"
                     /translation="MKKILGAIGGFFVAIWRWIKETAWVQPLLIVGIIFGIIFAIPSV
                     VDGIRKIDERNNSAEKYYQQFQVSLAGAENSAADKLLDEIKQNSEGGSESLKGQKFFV
                     VFVQKDEACSACLDAREGFEYLADDGKALLDDGRKIELKTIFVDQELKRKDKEDWKKE
                     DSDPVDNYAETAFEAFLLRNAARFEEYAGDALNTHYYINDGITEQQVEDIESADVKRF
                     QTPTILQIDFTDTAPQPGVTNVFIGVQGAKKLDRAKYIADAWNYKGQFGPNYTV"
BASE COUNT         6202 a         2944 c         3612 g         6143 t
ORIGIN      
        1 atctttttga tatatattta ctatttaact tgattaagaa tagttaatct ccttaaatat
       61 aaattctcta cattcaaaat aacataacac tactgaaaaa tcaatgatgt gaacttcaat
      121 aattctaata aagtagtgta ttctacatta gtgacttaat aatagtatct ttttataatg
      181 ctcgtatcat tacttttatt gtaatttaca gacaaaactc aaggatgaat attatagtta
      241 tcattgacaa ttgacaagat ggaatgtata gttagtacga agttagcggc gactaacaag
      301 aatatcgccg ccttcttttt taccataaag gttagcgttc gctaactaat aagcttaagt
      361 aattaggagt tataaagcat aaatgattaa tcgaaagtta ctgaaattaa ttgataatca
      421 caaacatatt attttcactg tgatactcat ggtatgcgga ttaattacaa atattgtaat
      481 aactggatcg cttgtttatt taataaaaga agtcgctaat catagtgatg atagtagtgt
      541 atggacttat ttatggcctg cattaattgc tattatttca attattattc gttatttact
      601 ttcaattagt gttggttact tgacagatat tattggtcgt caagcaaaaa aagacctgcg
      661 tcaaaaagtg tataacaaga ttgtaagact tggcgtgaga actactgacg ggatgaaggt
      721 ggctggttta acgcaagttt cacttgaagg cgtggagcaa ttagatttat attactcaac
      781 ttatattcca caatttttct acgcactatt agcgccaata attttgtttt ttgtaacggt
      841 atggtttgat tggcggtttt cactcgtgct actagcagcg gtacccctaa ttccactttc
      901 cattatcgct atatctaaat acgcaaaacg gatctttgct aaatactggg gaaaatacac
      961 agcaatgggt gataaatttc tcgatagtat ccaaggatta cgcgaactta aaatctttaa
     1021 ggccgatgct tatcagcaaa caaagatgaa tgaagcaagt gaagaatttc gtgtgattac
     1081 tatgaaagtg ttagtcatgc aattagcgag tacaacgatt atggatattg tcgcttatac
     1141 tggcgctgga attggtgtat taatagcaat aattagtgtt ttaaaatggg gattatcacc
     1201 atttagtgcg ttatttttaa ttttagtcgc agtcgagttt ttcttaccgc ttcgtgcgct
     1261 tggctcggcg tttcacgttg caatgaacgg ggcaagtgct ggtcaaaaga tattatcact
     1321 gcttgcagaa gaagaaacgc catggggtac aaaagaagta acgaatgcaa atatcgaaat
     1381 taaagatgta acttttgcgt ataacggtca cgctaacgtg ttaaatgatg tatcaatgac
     1441 ttttaaagag caaggcatgg tttcgattgt tggtgaatca ggaagtggaa aatcaacaat
     1501 tgttaatctt attacaggtg cacttagacc gcaatttggg agtgtgagtg ttggtggcga
     1561 attattagaa actctgtcgc gtgaaagtta ttatagtaat cttgcggtca ttagttacaa
     1621 tacttatatt tttaatgata caatccgcaa taattttaaa ttagcaaata aagatgtaac
     1681 cgacgaagag atatatgcgg ctttaacaaa agttaatctt gctgattttg ttgaagcaaa
     1741 tggtgggtta gataaagtta ttaatgaaga tgcgctaaat atatcgggag gagaaaggca
     1801 acgccttgct gtggcaatta atttagtcac taacaaagcg atatatatat ttgatgaagc
     1861 aactaacaat attgatatag aaagcgaagc cataattatg gctaatatta aagaattatc
     1921 gaaaacgaaa gcggtaattg ttatttctca tcgtctcgct aatgtagtgc ctagtgatta
     1981 tatttattat atggaaaatg gtgaattatt agaaagtggt aaccatgata ctttaatggc
     2041 gaataatagt ggctacgcta aattatttac gacccaaaaa gcattagaag aaggttacat
     2101 ggagtgtaat tatgaaaagg cctaatgtaa tacgaagaag tggggcaaaa attatggcaa
     2161 gtcttgtcgt acttttaggt agcttatctt atattatggt tttagcaatt attactggct
     2221 cattaggatt tattttcgcg atgggtgtca cacttttagg agcagtagcg atagctaagt
     2281 ttttaggtga agcaattgca ctttcatatc cgctaatttt cagtttgtta attggatttg
     2341 gtgttttacg cggaatcctt agatacatag aacaatacac aaatcattat attgcttttc
     2401 ggcttttagc ggtgctacgc gataagatat ttagtgcttt acgaacatta tttccttcaa
     2461 agatagagag taaacaaaaa ggatcaatta ttgcgatgat aacttcggat attgaaatac
     2521 tcgaggtgtt ttatgctcat acaatctccc ctatttttat tgctatagtt gtgtcgatta
     2581 gtgttatttt atttgttggt ttttcttcaa gttggttatt agcggtaaat gccctaatta
     2641 gttatattgt catcggtatt ttactaccac ttattacatc aaagaaatta aaagttccgg
     2701 gtgttcgtta tcgccaaaac tttgccgcct ttaactcatt tttccttgat agtattaaag
     2761 gaattaaaga tattgtttta aacaatggtg gaccaaaacg tgaagtagaa gttaatgaac
     2821 gttctgatat gttgcttaac gaaacaaaaa agttgaaaaa agatacgact agggcacacg
     2881 ctactactga actatttgtt tccatcttta tctttattac attagtgctc ggggcgatac
     2941 ttgttattcg tgataattta actattgggc gtatgattat tggcgttgct gctatcttcg
     3001 gttcattcgg tcctgttatc gctatttctt ctttaccaag taatttaacg caaacatttg
     3061 cagcaggcga tcgtgtactt gatttattaa atgaaaaacc actgattact aagattgata
     3121 acggaagtga ttttgaattt gaaaatgtaa cagttagtaa tgtgtctttt gcctatgctg
     3181 aaaatgaaga aatacttaat gatgttagtg tacacgctaa taagggtgag atagttggaa
     3241 ttgttggtcc ttcgggatgc ggcaaatcaa cattactaaa attgttatta agattttaca
     3301 aaaaagatga aggtacgatt ttatataacg gtattgcaat agaaaatatt aacagtgata
     3361 accttttaga taatgtaaca atggtcagcc aagacactta tctttttgac gacactattc
     3421 ttgagaattt attaatcgct aaacgtgatg caacattaaa agaggtggaa attgcttgca
     3481 aaaaggcatc aatttacgat tttattgttt cacttccgca aggttttaat tcacgtgtag
     3541 gagtgtttgg cgataatata tccgctggtg aaaaacagcg tattggtcta gctcgtgcgt
     3601 tcttgcgtgg tagcaaatta atattacttg atgagccaac tagtaatgtt gatagtataa
     3661 acgaaggcat tatcctaaga gcattaaaaa aacataaaga agatcgcggc atcattcttg
     3721 tatcacaccg tgagtcaaca atggcaatcg ctgatcgtgt ctatcgtttt aatggtgaaa
     3781 tagtagagga agttgaggat aaataaatga aagcaaaaaa gatattttgg attattttag
     3841 gttgtgtcag tctcgcactt ggtgcaatcg gagcagcact tcctatttta ccgacggttc
     3901 catttctttt gctcgccgct tttagttttg ccaaaagttc gaaacgttta cataattggt
     3961 ttattaatac aaaactatat aaaaagaatc tagcaagtta tgttaagggc aaaggcatga
     4021 cttggccggc aaaaataaag atgatgatta cagtgacact tgtgatgtta cttggattta
     4081 tcccgatgat gattaaaaaa gtatatattc ctagcattat tttaggagtc gtgtggattt
     4141 ttcatatcat ttggtttatt tttggtatta aaacttataa gaaaaatagc gaagactcgc
     4201 tttcaaccga agtagaaaca ccaagcgaaa ccgaagaaaa aaacattgaa taaaatttaa
     4261 aaagtaagta aaaaatataa ataaaatagt ataaatagcc tttattttca cacaaatggt
     4321 gtaatattat aatgttgcct taatctaaag attaaaggag gaagagcgac gatgaaagta
     4381 gaaaatattt ataaacaaaa atatgtgctc accactaatt attatgatcc aagtaataat
     4441 attggtgtac aaggcacact tgatttattc caaaatattg ccgcccgtca cgcttattta
     4501 ttaggtgccg gtcgtgaaca cttagaaaaa attaacttag tgtgggttat agcgcgcaca
     4561 gaaatagagt atgttactta tcctgaaact ccgctagaag ttgaggttgt gacttggcca
     4621 cgtcctgcaa caaggtttta ttttgatcgc tattataaaa ttgtaaaccc agcaacgaat
     4681 gaaataattg cttactccag aagtagatgg gttttaattg atattgattc gcgtaaaata
     4741 gtaggcgctg atcgttatga ttatccgctt gatttctatt atgaagaaga acctttcaat
     4801 cacaccttcc cgcgacccag tgttagtgat aatgttgtag gtaattacac cgtacgccca
     4861 agtgatattg atgaaaacgg acacttaaat aattcaaaat acggaaatat aatctatgat
     4921 tatcttgatt tacaaaaagg tcaaaagatt aaaagtattg taattaatta taataatgaa
     4981 gctttacaag gtgaaacctt aacaattagt cgcagcaggg acgatgcaaa agtcgtaatt
     5041 tttgggcata aaggtgatac aattgtgttc agtagcgagg tcaatattca gtaatgttaa
     5101 caacacttga actatggatt attattatcg ggatggctat accattcatt gcgactgcgc
     5161 ttggtgcagc agcggtattc tttttccgcg ggcccataag tgagcgagtt aatagtattt
     5221 tactaggatt tgctgcgggt gtaatgattg ctgcttcagt gtggtcatta ataatgccaa
     5281 gtttagaatt tgctgaaaat tcaggctggg gtaaattagc gattattcca gttgcggtcg
     5341 gatttgcatt aggagtactc tttttatggt ttattgaccg tattgtccct catatgcatc
     5401 aacgtagtga agatgaagaa ggattaccaa ctaaaaaact aagtcttgat tcaaaattat
     5461 ttttagcaat gacaattcat aactttcctg aaggtattgc ggtcggcttt gcttttgctg
     5521 tggcttatac aggtattgtt aatcctggaa atgcttctat ttcgctctat agtgccctag
     5581 ccctctcaat tgggatggca attcaaaata ttcctgaagg agcggctgtt agtttaccga
     5641 tggcgcggcg tcttaataat aaaggtaaag cattcggatt tggtgttgct agtgcaattg
     5701 ttgaaccaat tggtgcagtt ttggcgtttc tatttgccac ggtgtttcaa acactcttac
     5761 cgtggttttt aagttttgcc gcaggagcga tgatgtttgt tgttgtggaa gatttaattc
     5821 ctgaagcaaa tttaagtgga actgagcacc aaggcacatt tgctgtaatg ttaggcttta
     5881 ttattatgat gattatagat atagtattag ggtaataaat gcgctcgtag ctcaggtgga
     5941 tagagtacaa ccctccgaag gttggggtca cacgttcgag tcgtgtcggg cgcgccataa
     6001 agaaagagaa aagcacccag tcgcgggtgc ttttttgttt attgttatta gtaactattt
     6061 aatatgttca gtaacagctt caccaaataa gtgaccaagt ttaatagtac catcactatc
     6121 ataatgtgcc cagtctattt caaaatccgg ttcattgcta aaagaaagtt gtggggtaag
     6181 tgtatcgaca atgatgtttt ttggatcact ttttgctaca ttacgcttag cggtattaat
     6241 tatttcgtga tgtgtccaaa attcagcatc attaataagg ccgtcaataa agtaaatgcc
     6301 tgctttaggt gcgtaatctt taaattcgtt ccgtaaatca ttaacaagat tttgtaaata
     6361 cttttcgtat tgaagatgcc caccatcaac tgaatcattt tcaccttgca tataaagaat
     6421 agcgctgaca gttggattat agccatcacg ctttaatttt gtaagcatac ggtgcgcata
     6481 attagtggca aacacataac atagtccagg gatcccactt gatggtgagc gccaaaatac
     6541 ttcaaggcta gtgccaccaa tcgcgaactt aataatttta aattcatcac tgtgctgagc
     6601 tttttctaag tgcgcagcaa tcccaatttc aggaccaaaa cgcgtagagt catatccttg
     6661 tccaactctg acagggacaa attcatcata tgattcatta ttaccattat cacagttata
     6721 ggcaatttcc gttttgaaag gggaagtgta gtaattaact aattcaggcg ggaaatgccg
     6781 gtctaaataa ctagtccacg aatggccttc catatttgat tggcctaaaa gaatgatgat
     6841 tttaatatct ttaggtttgt tcataaagtt tatttaacga taatttgtgt gccttgtaac
     6901 acatctagtg tcttagcgtg taagtctttg tcatagctat tgacaccgtc tttaaggacg
     6961 ataacttttg cttcaggtaa agcggcttta gcaagtaaag cattacttaa aatacaaata
     7021 tttgtcacta aaccaacaag cgtaatttct tcataacctt tatttacaat atcgttagcg
     7081 agttcaagtg aaccaaaagt taacttttca taaactttct ttgcttttgg taagtaggct
     7141 gctactttac catgaatatc atgacccggt gtacctttga tacagtgttt gaaaggtaaa
     7201 ttacgccctt cttcagtttc taaatagtta tcaaaatggc tgtctttagt gaagacaatg
     7261 tcatcttttg tttttaagtg gtcttcaata gtatcaatga ttttttgttc gatttcatgg
     7321 gcatcactaa agccaagtgc tccattaacg aagtcatttt gatagtccac aacaattaat
     7381 aaacgttttt tcgccatttg ttttcctcct tcatggcgga gaaagaggga ttcgaaccct
     7441 cgcgaagatt gctcctccta tcggttttcg agaccgaccc cttcagccag gcttgggtat
     7501 ttctccgtaa tgctaattat aacaaagatt ttatagtatc tataaaatat tcgcttctac
     7561 gcaatgataa aattattttt atgtataata tacataggtg atttatgcag gacattatta
     7621 atttcatatt agaaaactta ccaagggtaa tactacttag cggcacaata gtgatgttag
     7681 ttattgctgt gcttttaatt attcacggta atcgtaaaga tcttaaagac caaaacatta
     7741 ttttaggaaa tataacaaca ccgcggattt ttcttcttga cttaggtgaa ggaaaagtca
     7801 cttactttaa tcgcggtagt tttggcgaac gcaaaaatgg tttattacca caattttttc
     7861 aacaatttac cgcgaaagaa gttgatcgac ttgataaatg ggttaacgct ttaattgatc
     7921 caaaaagtga agcaagtaaa ttttataaag ttgatgtttt tgtgcgagat atgcgtcggt
     7981 catacgcttc aattttagaa gtaactggtg tcgattatga aaaaaagatt attcaccttg
     8041 aatcttattt gtatcgatat ttaaaggcta atgaccaaat gaatttaacg gtgaagaaaa
     8101 aagagaatat aggttactta actgaagaag aattagtaaa ggtatacaga cgcgcccata
     8161 agcgcgggcg tggcgtatat ggtgcgatta gtattgcagt gacagcaagc ggagcacaaa
     8221 cgcaccgcgc agaaataccg ccgcatttat ggtatgagtt acaaaaccaa gtcgctgttt
     8281 atcggaaacg aagtcgcgtc attcgttttg ttgataatca tactgttggc atatacttcc
     8341 cgcgttcaag tgatgttgat accgctattg atgtgttcca atcaataaag aaacgcatga
     8401 gcggttatct ccaaaaaaat ggctacaacg aatattcact cgggattggg ttaacgcggt
     8461 ctcgtcaatt ttcggagatg aatatgtttt ttaaaacagc ggaagaatta agtctttatg
     8521 cgcttaaaga agacggtcgg ctcttaattc atgatccaaa taaagaaatt aaagacatgg
     8581 acttaagttt atatcgcagt gaactaacac aacttgtggc tcgcaaggga gtcgatactt
     8641 tatttcaacc aattgttaat gctaaaacag ttgaaatatt agggtactta acgaccttca
     8701 gcatcagcgg ttcattattc gggagtttca atgaagctgc gcaatttgcg cgagaaaata
     8761 agcaaaacga acagcttgta aagatggtgc tccggaaggc agcggcccgt tattacaata
     8821 tgcgccgcaa taataagcaa ttactatttg tcccccttgg gttaggtgga gtcgacttta
     8881 ttagtgatgc ttttagtacg cagccatttg cgaatgatct caaaatggtc tttatgttag
     8941 aagaacaaga tttcgctaac acatttgtga acattgaaga tatgttaaaa ctgattaaga
     9001 aaattaaaaa acaaaagtat gaagtgtgtt taatcattag tgatactgaa ttaacactgc
     9061 cagacaaaat ttatggtgaa gttgattact ttatggtgaa tgaaaaaatc ttaacccata
     9121 cagttaatga tgaacgggaa cgcttatcct tactttcatc attaggtaaa ttattacgtc
     9181 atcaaaaacc aataatgatt agtgatttgc caaagtggag tgaaatcgag tattatattc
     9241 gggcaggtgt agattatgtc gctagtgatg aaattagcaa gaaagacaac agcttgcaaa
     9301 ctattgataa aaagaaagca ctaagaatta ttaattttag taagaggaaa taaagatgag
     9361 taagattaaa attgatgcaa ttacaaccca aagtgaagat tttgctcaat ggtacactga
     9421 tgtgtgtcgc aaggcggaat taatggatta tagcgaagtt aaaggtttta ttatttaccg
     9481 accttatggt tatgcgattt gggaaaatat tcaaaaatat ttagatgaaa agtttaaaga
     9541 aaaaggtcat gataatgtgt acatgcccct tgttattccc acaagtcttt ttcaaaaaga
     9601 aaaagatcat atcgaaggtt ttgcccccga aacattagtc gcaacacaag gtggtaatca
     9661 aaagattgaa gatccactta ttattcgtcc gacaagtgaa gtattatttt gtgatcacta
     9721 cgctaaaata gttagctcat atcgtgattt accaaagaaa tataatcagt ggtgttctgt
     9781 cgttcgctgg gagaagacaa cgcgtccttt tttacgcggt agtgaattct tatggcaaga
     9841 agggcacacg attcatgaaa cggaagtaga agcacggacc catgcgcttg atatgcttaa
     9901 agtttatgaa aacatgggta aagaattact cgcgattccg tttttaaccg gtactaaaac
     9961 cgagaaagaa aaatttgccg gcgctgaaca aacttttaca attgaagcac ttatgccaga
    10021 tggtaaagct ttacaatgtg gtacaactca ctactttggt gatggttttg cccgtgtttt
    10081 taatattcaa ttccaagatc gtaataacac tttacaatat gtgcatcaaa ctagttttgg
    10141 tgtttcaacg cgtttaatcg gcagtgttat tatggcgcat ggcgatgata atgggcttag
    10201 cctaccacca cggcttgctc ctgttcaagt tatcattgtc cctatccaac aaaataaacc
    10261 aggaattatg ccaaaagcct tagaacttgt cagtcgctta aaagcaattg gtgttagagt
    10321 taaaattgat gactccgata agtcaccagg ctggaaattt agtgaacatg aaatgaaagg
    10381 tatcccgctg cgcttagaaa ttggtccgcg cgacctaaag gcggaagttt taagtggttc
    10441 aattcgtcat agccatgaaa aaattagttt tgccgataaa gatttagaag aagtagtgcc
    10501 ggcactctta gaaaagattc atgaagatat gtatcaaaag aaacttactg atttacttaa
    10561 tcacaccatt gaagtaagaa gtattgaaga acttgaagat gcccttaaca acaaattagc
    10621 gtacgctaag atgatgtgga gtggtgatca agaagactta gatgtgatta aagaaaaata
    10681 tcaagcaacc gctagagcct taccttttaa tcaaactcca tttgctaaaa agtgtttaat
    10741 cagtgggaaa cccgctaaat atgttgtaat catcgctcgt gcctattaat taatttacaa
    10801 aatattaagc atgtgatatt atttagcaag acggagtagt acccaagttg gtgaaggggc
    10861 ttccctgcta aggaagtagg tcgggtaact ggcgcgtgag ttcgagtctc acctactccg
    10921 ccattagtta atcagcagat ttgatgaaaa tctgcttttc tttattttga atttaaacca
    10981 caaacaaaaa tgtgataaat atgttttaaa taaacatttt aacaaaaata aaaattaaga
    11041 attatagcgt ctttattatt attattatta tatattggat aagatttcac tgaatttctt
    11101 caaaaaacaa tatgaaaaga gattaaacaa tattgaataa tcgaaaaatg cgaaattgaa
    11161 ggaaaacaaa aaaaacatta agggtaataa ataggagtta aagatgatat taaacgaatt
    11221 attcaaacaa cactcaatat tacttttgtt atttgctgtt ttagtatgga ccattttcaa
    11281 gtttcccaaa attgtaaagc gccaaaaagc taatacaaat aatagtagaa tgtacttcgc
    11341 aaaaaaacat tataatcata gtgggaaaac tagaaaacag gtattaggta aggataatga
    11401 tttaaaagtt aatgagctac cattattcaa tcacccaaat aagaaagagt gttttgtaat
    11461 aactaaagca atgcatgata gaattggaaa acataaattt ataccattat ataaaagcgg
    11521 agataatatt gctggagtaa tacttacaag taaaagttct tatggcaaga aaaaaaactt
    11581 tcaaataaaa ggtggttatt taggacatga acttcatcac tgtattgcaa aaattaattt
    11641 aacaaaaaaa gatttacgtt attctaataa tatttcatat gacgaatcta actatttaca
    11701 atttttaaaa gcgaataaaa agaacttaga aaaatataat gagttcataa ataaaaagca
    11761 gttatccaaa taactgctgg aatgatgcga tcagcccgct tacccgcaaa taatgagggg
    11821 cggtttaaat acatattgta aatgctgata ctgatccctt taatacggcc ctaggtttac
    11881 gtgaatgcat cactctcact acttagtggg taagaccgcc aagattcctc ttgcatcttc
    11941 attattagat attattagtt aaatgtcaag ttttagaata aataatattt ttttgtaata
    12001 aaacaacatg taaaagttga ttaaataatt ataaaaatat catttaattg taaaaaaata
    12061 tcactatttt gtacctttct aaggcgcgag atattaattt ttgtataata tatacaaagc
    12121 cttgattatg cattaaaaaa aggatgcata ttgatcagag ttaaaaagaa agaggccaat
    12181 agaagatgtt cttacaaaac ttattagaaa cccttattaa actagatgta aaaacaatga
    12241 tgggtgtttt aatttgtggc aatttagtgt tatcactttt aacacttcgt tattacatat
    12301 atcacgattc aaattatgat aaaacaataa tccaaagatt tggtattgct aaaattttac
    12361 aagcaatcgc gtggttattt ttatttttta gaggggaaat aagtgatgtt atttctgttt
    12421 actttggtaa tatattgctc tttgtcagtt tttatttaga ttcgttaatt gttttacggc
    12481 taaatgataa gtggacaaag ggctgggtga taacacaaag tgttttatta gcggtagcag
    12541 ttatcttatt tttagttttt gaaattacgc ttagcgctgc gcatattagg gttgggattg
    12601 cttctttagc ggtattttta gtttttgccg cccctaatgt tttaagtatt ttaaataaag
    12661 agagcacgaa gtttcaaaaa gtcattgccg caataagtct gctcttcctt atcttcctct
    12721 tattacgagc atttaattcc ctatttgata atgagattgt acttttcact aataatttct
    12781 tccaaattgg tacattcctt gccttagtgt tattaatgtt aactaatggc gtaggatttt
    12841 tacttataat gtatgaccgc tcttatgcat tagtgaaaga aagttctaat ttagatccgt
    12901 taacacgcat ttataatcgg cgctttttat gaataaagct gagtcctatt ttaagcgggc
    12961 acagcaagaa gatggttact tatcatttct ttttattgat attgattttt tcaagaaagt
    13021 aaatgatact tatggacact tatttgggga cgaagtatta aaggttgtag caaagacaat
    13081 cgcagaaaac attcgaccat tagatctttg ctgcagattt ggcggggaag agtttttaat
    13141 ttatttacat gacactagta aagaagaagc gatcacaatc ggtaatagaa ttcgggaagt
    13201 agtgcaacta ttagaattta aacaagttaa agaatataaa tgtacaatta gtgtcggtgt
    13261 atattccgct aagccaaaca ccgaaacaaa cctccaacag tttattgata atgttgacca
    13321 agctttatat aaagcaaaag cttctggtcg taattgtgta atgagttttg attaaataaa
    13381 tatagaggag caaagttatg aaaacagcaa tgatgacagg ccatgtgaca cgctttggtg
    13441 atttagagtt tgctttacga cttattaagg agttaggttt tgaagctgtt gatttaacaa
    13501 tgtatgacac ctttggtggt gttgctgttc gtaatcgtga gttatttact ggtgacataa
    13561 ttgcaaacgc gaaacgcatt aaaagtatcg ctgaagagat taatcttccg attgtgcaag
    13621 cacatacccc gtttcatgtt tatcgtaagg acgatgaaga atataatgca tacatgttag
    13681 aaacacaaaa attatgttta caaatagcgg gcatcattgg cattccttat gttgttattc
    13741 atccagtgat gaacgctgat aatgagttta ataaaaagtt ttttgatgaa ctacttccgc
    13801 tagcgaaaaa aatgaatgtt gtcattgcaa ccgagaatat gtgggaatgg caagtaggag
    13861 caattcattg tgattactca acttgttcaa ctcctgaatc atttttaagc cttttagaat
    13921 atattaatca tcctaactta aaggcgtgtg ttgatgttgg tcatgcgcaa atgtttaatg
    13981 gttttgataa aagtgtatca ccgaaattaa tgattgaaaa acttgctcat aaatattgtg
    14041 ttgcactgca tcttcatgat aatgattgtg ttaatgatta tcataaaatt ccgtttgatg
    14101 gtgtaattaa ttgggaagaa gtttatgatg ctttaacaaa gattaactat caaggtcatc
    14161 ttgttacaga aacgagttta ccccgtgaat ttaacctcga agaaagtaaa gagttttggc
    14221 ttaaacaaag agaagctgcg gacaaaatta aaagtgaatt acttaaaaga cgcgcttaat
    14281 gctctgatat tttaaataac attcgaacat aatgtttagt attatattgc cgaaataatt
    14341 ataatgttta ttgcgataac cttgcaaaaa ggcactcttt ttaatttggg attagttttc
    14401 gttaaaaaat agcgacaatt tacacatttt aaattgaaaa tggttgtgtt tacatgctaa
    14461 aatatttata caaatagacg ggggatttat tatgttttta atactttcag aaatgcttag
    14521 tgaattgcaa attcaggtta tacaactctt agttgttttt gtttttatag ttacacttga
    14581 tgtactttta ttaattacgc ttaaacgtat attccccaaa gttacgctta tcgtactttc
    14641 agttgtcttg ttagtgctcg gtttatttga tttaacatat gccttttact tagttgcttt
    14701 aatttatatg ttatgcgcta tgacgtttat ttttggtaat attgttgaat ttagatggtt
    14761 aatctccaat gccgccacac caaaagaccg tcgcttccag cgtgattttc gtaagattta
    14821 cgaccaagag gatttagtaa atcaacttgt atctgcggta ttttcgttat caaaaactaa
    14881 aactggtgcg cttattacta ttgaacgcac taatcaatta acggaattaa ttaaaaatgg
    14941 cagtccgatt gatgcaccgg ttagtgcacc tttattaacg actattttct ataaggggac
    15001 ggcacttcat gacggtgccg ttgtaattcg tgacgatacc attttagcgg ctagtgtcta
    15061 ctttacacca tcaaccaaag cacttgcagg aaaggttggc tcacggcacc gcgcagcatt
    15121 aggaattagc gaaattagtg atgctgttac gattgttgtt agtgaagaaa ctggtcgtat
    15181 ttccattgct tatcgcgctg ctttgcaacc tgtgagtcct gaagacttta gaaaagtatt
    15241 agtcgatcat ttattaacga aataaattat ttaaagaggt tatatatgaa attatttgga
    15301 acagacggaa ttagaggacc ggcaaatgtt ttactgacgc caggcttaag ttttgatatt
    15361 ggagcttttt taggtcaata ccaaaagaaa aataagattt taattgctta tgacacgcgc
    15421 agaagtggaa ttgttatttt tgctagctta gttgcgggta ttttaagcag tgggggtgag
    15481 gctagttcca ttggtgttgc gccaacacct gccttagcgt atttatgtaa aaaatacaat
    15541 tatgattacg gcatcatgat tagtgcatcg cataatcctt tccaagataa cggcattaaa
    15601 atatttgaca caaatggttt taaaattgct gaatcaatcg aagaggaaat tgaaaagtat
    15661 attgcaagtg acacacgact taaacttaaa acaggcggtg aaattggcaa atttaccgac
    15721 aaatccgagt taatttatga atatgttgat tacgctaaga aggcatttcg cggtggactc
    15781 aagcttaatt tattaattga tggcgctaat gggagcgcta gcagtgttat tcatcatatc
    15841 gttgcaggca ctagtttaaa tgccacaatt attaatgttg aaccagacgg tgttaatatc
    15901 aatgcagact gcggttcaac ccatttagat aaactacaag acacaattaa aaagtcacct
    15961 ggtaaatatg acctcggtgt tgcttttgat ggtgacgctg atcgtgtttt atttgttgga
    16021 cccaaaggtg aagtacttga tggtgaccat gtattatatc ttttagggaa acattataaa
    16081 aaaatcggca cattgacaaa agatactgtc gttgccacag tgatggcaaa ttacggactt
    16141 ggttcagcat taagtaaatt aggtattaaa ttagtcgcca ctagtgttgg cgataaacac
    16201 gttcaacggg aaatgattga acacgattac ttaattggtg gtgaacaatc aggtcataca
    16261 atatttaatg gttatattaa gacgggtgac ggcatttata cgatgatgaa agtactagat
    16321 gtcttagcgg cagaaaaaac aacatttagt gaatatgtta aagaatttat caaatttcca
    16381 caatgtttat taaatattag agtcaaagat aaaaagatgg caatgactga taaaacattg
    16441 cttgcggaaa ttaaaaagag tgaagctaaa cttggtggtc atggtcgtat tttagttcgc
    16501 gctagcggaa ctgaagaatt agtaagagtg atggttgaag ctgaaaccga agcggaagca
    16561 aaagcaacag cagaacactt agcgtctttt gtaaaataga tgtaagttgt aaattaaaaa
    16621 attataatta tttaattcac atcctaaata atggggtata attttattta tgaaaggggg
    16681 catgtcaatg gaattaaatg aaaagataat tgcacgttta attgaacccg ctgacattgc
    16741 ttttcttaat caaaaacaac catttgactt agcggttctt tacggtgaat tagacgaaga
    16801 gagccaagca tatttactta gtttattaac tgatgaatca cgcgccgatc ttttaagctt
    16861 ccttaatcca gaagacgctg ctgaattttt ggaagaaatg gagattgaag aacaacaaaa
    16921 tattttaagt tactaagtta cgacgctgat agtgcagctg cggttagctc gccacacttt
    16981 atttctttcc caaatacgac aacagttaaa aaagcaacgt ataatacgat tgctaatgcc
    17041 gctgataatg atgaaattga tgttattttc ttccatgatg aaaataataa atatgtaggg
    17101 gctactacca ttaaagactt aattgccgct cgttcaacaa ctccaattag tgaagttatc
    17161 gacactaatt tcccgttcgt gtatgaagac gaaaaattta ctagggcatt aaaaatgatt
    17221 cgtgattatg acatattaat gcttcctgta ttaacgcgtg atggtgaaat gattgggttc
    17281 gtttctgaaa ctgacgcatt aaatttaatg gcggaatcgc atgaaacaat gatgcgccaa
    17341 ttagtgaaag ttgccgaaga tgatgataat cttccaccac ataaaacgcc ttaacacggt
    17401 taccgtggcc gctcgtcgcg gtcgtactta acttactaat cgctggcaca cttacatcat
    17461 acgaaggact tttaagtact tttgtgggct tagtcttatt ccaaccaatg attcttggga
    17521 tggctggtaa tattggtaca caaagtatgg gggtcacaat tttaagctta catagtggtg
    17581 aaattaaaat tagaaaacat tttgggcgtg aacttttaat tggattatta aatagttttg
    17641 cttccggtat cgtcgggttt ggtattgttt tcttattcct tactatttta ggtcaagata
    17701 atgctttgat gtatgctgca gtagtaggct tatcacttat gtgcgcaatg attattagct
    17761 cattagcagg tgtgttaacg ccaattattt tgctcgctgt taagctagac gaaaaagcag
    17821 cgtctggtcc gcttattaca acgattaatg acttctttgc cttacaaatt tatttcttaa
    17881 tcgcgacact tttgtttaca gtactttaat aatttagtag aagaagtaaa aagaaaagcc
    17941 ggcttaacgc cggcattttt caacttttta ataatgttag acagtataat ttggtccaaa
    18001 ttgaccttta tagttccatg catcagcaat gtatttagcg cgatcaagtt ttttcgctcc
    18061 ttgaacaccg ataaagacat ttgtaacacc aggttgtggt gctgtgtcag taaagtcaat
    18121 ttgtaatatg gttggggttt ggaaacgttt gacatccgct gattcaatat cttcaacttg
    18181 ttgctcagta ataccgtcat taatataata gtgggtatta agtgcatccc cagcatactc
    18241 ttcaaaacga gcagcgttac gaagtaagaa agcttcaaaa gcggtttcag cataattatc
    18301 aactggatcg ctatcttctt tcttccaatc ttctttatct ttacgtttta attcttggtc
    18361 cacaaagatt gttttaagtt caatcttccg gccatcatca agtaaagctt taccatcatc
    18421 cgctaaatat tcaaagcctt cacgagcatc aagacatgca ctgcatgctt catctttttg
    18481 cacaaaaact acgaagaatt tttgtccttt taagctttca ctaccacctt ctgaattttg
    18541 cttaatttca tcaagtaatt tatcagcggc actattttcc gcaccagcaa gtgagacttg
    18601 gaattgttga tagtattttt ccgcactatt gttacgttca tcaattttac gaatgccatc
    18661 gacgacactt ggaattgcaa aaataattcc aaagataatt ccaacaatta aaagtggttg
    18721 gacccaggct gtttctttaa tccagcgcca gatagctaca aagaaaccac cgatagcacc
    18781 taagattttt ttcatgaaaa gttcctcctt ctattaaata atagagcacg tataataata
    18841 acactctcaa catatatttt caaacaaaag caataataaa aaaagcaatt tactaaagtt
    18901 t
//
LOCUS       DAOSTA010000018        18786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_3100_length_18786_cov_7.096845, whole genome shotgun sequence.
ACCESSION   DAOSTA010000018 DAOSTA010000000
VERSION     DAOSTA010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 18786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18786
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3100_length_18786_cov_7.096845"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            341..2926
                     /locus_tag="PLU08_02835"
     CDS             341..2926
                     /locus_tag="PLU08_02835"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOQ70665.1"
                     /translation="MKLDTHYNHLKVEKGRYEHWKKSGYFKSGDTSKPKFSVVIPPPN
                     VTGVLHLGHAWDNTFQDIIARYKKAQGFDVLYLPGMDHAGIATQARIDGRLRERGLSR
                     YDLGREKFLEEAWKWKEEYASTIRSQWAKLGLMLDYDHERFTLDKGLNDAVKHTFVEL
                     YNRGYLYLGERIINWDPVFQTALSNIEVIHKDVEGSFYYFKYKLEGSDFYLSVATTRP
                     ETMFADVALVINPRDERYTKYLGKNFVNPVNNELIPLIADDYVDLEFGTGVMKCTPAH
                     DANDFKIGERHNLSRPICLNKDATLNERGLELAGLDRFDAREKLVAKMKELDLLIKVE
                     KIVHNVGHSERSDAIIEPYLSKQWFLRMDHFAKKSIEGQKGDDPIRFFPKRFNKTYLN
                     WMEIAEDWCVSRQIWWGHRIPAYYHKETGEIVVSVTPPKDLENYRQDEDVLDTWFSSA
                     LWPFSTLDWPNTDSELFKRYYPTDVMVTGYDIIFFWVSRMIFMGLELTNEKPFKDVVI
                     HGLIRDEQGRKMSKSLGNGVDPIEVIAKYGVDSLRYFLAGASTPGQDARYSEARIQAS
                     VNYLNKIWNAARFLLLNLPEDFKPGKINHKKLHPVDAFILSRYYETVRKVTSLMDKYE
                     LGMASNYLYAFVYDDYCSNYIEMVKVILNSDGDHTNTYNVMYHTLHGILMMIYPYSPF
                     ISEEIYLALPNHLDSIMLASYPKASRLRFKREAEEVNTIINLIKEIRNFKSTNNLAPN
                     TKMNLYVADVSFDLTPYLDIIRRLGFINEFKYSDVPIEVTTVSLPKATFGLELDVDRS
                     VLISQLEKEIETLTKEVERSTKLLNNPGFKNKAPASKVAEEEEKARHYAESLALAVDK
                     LTKLR"
     gene            2969..3145
                     /locus_tag="PLU08_02840"
     CDS             2969..3145
                     /locus_tag="PLU08_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70666.1"
                     /translation="MTLLTNAEMANIKGGEPITLAAVMTILVIAIVTVIVYKLFTSNA
                     GSTTIPGGFKFEWK"
     gene            3132..3818
                     /gene="radC"
                     /locus_tag="PLU08_02845"
     CDS             3132..3818
                     /gene="radC"
                     /locus_tag="PLU08_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOQ70667.1"
                     /translation="MNGSKRIKELPHLKRPREKALYYGIGSLSDAEVLALLIGKGTTK
                     DNALSLAKKLLNKHISLQNMYRIDDPKLLLMPGIGEVKALQILAAFELNKRLLFLEGS
                     HDRPIYREDEIVRSFQRRIGRLQKETLYLLALTNRNYIISEKQLYLGTEKGFSLDPKE
                     VVRELLIANADRYILIHNHPSGHVEPSANDLETTIELATISAQFGVTLYDHLIIGANA
                     YFSLRKLGKL"
     gene            complement(3880..4716)
                     /locus_tag="PLU08_02850"
     CDS             complement(3880..4716)
                     /locus_tag="PLU08_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOQ70668.1"
                     /translation="MINITSIKAKTRNLASQIGLSANIILRMYFFERFLMRLSKSLYK
                     DKFIIKGGFLISSIIGITNRTTIDIDTNIKDMNLEQEMITNVIKEIINIDINDGVEFI
                     FTNIEIIREIDYYDNFRISLVAMLENTRIPLKLDITTGDPITPSKIVYEYSCLLTSEK
                     IRLYAYNIETIIAEKYETIIVRNIETTRMKDFYDVYMIFKLKHNAINFKTLKRAIFNT
                     SNKRKTTKIIKDSKEILNNMQNNSKLINEWEKFMQKNSYAADIKFFDTIEILKTIADI
                     IV"
     gene            complement(4713..5300)
                     /locus_tag="PLU08_02855"
     CDS             complement(4713..5300)
                     /locus_tag="PLU08_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOQ70669.1"
                     /translation="MTSLQNYLNERPVITNKDAARIGVNRQLLSVLVKEGKLERLRPG
                     VYQKAGEVIDDFMLVSANSNQVCFSHETALILHDLSDRMPDRLYITVPQGYNTSHIKK
                     RYINLKTRYVKRKHHKIGIITMKTYFGNKILVYDMERTICDIIADRENIEKEVFVDAI
                     TRYFRRKDKDIKKLIKYSRQFKIENEILRYIEILS"
     gene            5653..5961
                     /gene="rplU"
                     /locus_tag="PLU08_02860"
     CDS             5653..5961
                     /gene="rplU"
                     /locus_tag="PLU08_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011247445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOQ70670.1"
                     /translation="MYAIIETGGKQVKVATGDVIYVEKLDVAEGEDFVFDKVLLVGGK
                     KAKVGTPYVKGATVSAKVVKHGLNRKIKVFTYKNKSNVRRMLGHRQPYTCLEVVEIKG
                     "
     gene            5964..6287
                     /locus_tag="PLU08_02865"
     CDS             5964..6287
                     /locus_tag="PLU08_02865"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOQ70671.1"
                     /translation="MINVVITTIDEEILKIRIKGHAYSGEPGHDLVCAGVSAVSIGAL
                     NSLHEVDTAFKVTVEDGLVEIVPLYRPSYENKIVLQVLITSLKTIAESYGKYVQIREE
                     RRAEA"
     gene            6284..6568
                     /gene="rpmA"
                     /locus_tag="PLU08_02870"
     CDS             6284..6568
                     /gene="rpmA"
                     /locus_tag="PLU08_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016338754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOQ70672.1"
                     /translation="MKFILNLQLFASKKGVGSTRNGRDSTGRRLGAKRADGQLAKAGA
                     ILYRQRGTRIYPGLNARRGSDDTIYATVEGIVRFERFGKTRKRVSVYPVN"
     gene            6657..7619
                     /locus_tag="PLU08_02875"
     CDS             6657..7619
                     /locus_tag="PLU08_02875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ70673.1"
                     /translation="MAKKLVTVVVPMYNEEPMVPHFLTKIAEIIKENDGYNFEVVAVN
                     DGSRDNTLALLYDFQKKYKFLRVVNLSRNFGHEAAVAAGLSVAKGDAIIPIDADLQDP
                     PELIKPMLELFEEGYDVVNARRASRKEDTWIKRYTAGKFYRIIDKLSGKVRIPQNVGH
                     YRLISRRVLDEVLKLPESNRVFRVEVPYVGFKTAEVAFVRPKREHGKTHYNMKSMIDL
                     AINAITITSTKPLNFVFKFAMWLGIIFGLSAVTQLVLFILEMTKVLVLPFLNHGVWLL
                     INVSLFLTTVILLALTIISIYLGKTFNETQKRPFYVIESVKENK"
     gene            7623..8906
                     /gene="obgE"
                     /locus_tag="PLU08_02880"
     CDS             7623..8906
                     /gene="obgE"
                     /locus_tag="PLU08_02880"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOQ70674.1"
                     /translation="MFIDQAKITLKAGNGGNGMVSFRREKYVPKGGPDGGNGGRGGSI
                     YFVATSNISTLTDFRFGLTIKANNGGNGATKNQYGKSGEDLYVKVPIGTVVYLEPSRE
                     VIADFKLEGETMLIAKGGKGGRGNAAFKSSTNRVPRIAEHGVPGEELLVTLELKLLAD
                     IGFIGLPNAGKSTLLTVISEARPQIDNYPFTTLRPELGVVKANSGNRFVAADLPGIIK
                     DAHLGRGLGLRFLRHAQRCKVLAFIIDGENSEDYYKEYKLLRNEVKEYGYKLDERPFF
                     VAVSKDETVDTHKKVKALAKKLKDIKVLGFSSILHENIDTLINTMEEYIVNAPEIEMV
                     DEEGVAVKYREYDLTATIDPDLFTIEKVRAGVYRIVGEKVEERYHKYILNTEEAILSL
                     LNYLREIGVEDALDKMGVNDGDTVILVDFEFEYFR"
     gene            8916..9644
                     /gene="nadE"
                     /locus_tag="PLU08_02885"
     CDS             8916..9644
                     /gene="nadE"
                     /locus_tag="PLU08_02885"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOQ70675.1"
                     /translation="MGQESLQNYLQYLVRYLKRRARGFNGYILGLSGGLDSAVVALLA
                     QKAVKDDLLCVIIDIESEKCDMDDALELCKLHNLNYYYIDLTAEYRAVVKKLEESKKL
                     TELSKINTKVRLRMVTLYALGQTHSKLVLGTDNKAELFTGYFTKHGDGAADLFVLSSL
                     TKGEVFAAARLLGVTPNIINKIPSAGLYVGQTDEAEMGIKYEDLDAFISGKEVSPSVK
                     ARAIELHRISNHKRRPIPRPREKR"
     gene            9742..10299
                     /gene="ruvA"
                     /locus_tag="PLU08_02890"
     CDS             9742..10299
                     /gene="ruvA"
                     /locus_tag="PLU08_02890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOQ70676.1"
                     /translation="MIYGLKGIVTKQTATTIVLDVRGVYYEVKVPSVPQQQQFSELFL
                     YTYQVIREDEHYLVGFLSLEEKSLFTELLNVSGIGPKTALGIMTATTPERFLQAVTSE
                     ELSYLKKLPGVGPKGASQIILDLKGKLQTDTSKGLNPYEQEVTLGLQSLGFKKSEITK
                     VLKKVSNKSLDANELLRLALSELRR"
     gene            10299..11306
                     /gene="ruvB"
                     /locus_tag="PLU08_02895"
     CDS             10299..11306
                     /gene="ruvB"
                     /locus_tag="PLU08_02895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009361711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOQ70677.1"
                     /translation="MSNRLVSDKLDQSEASVELSLRPLTLNEYVGQTALKHNLNIFIT
                     AAKARNEVLDHTLLFGPPGLGKTTLAHIIANEMGANIKVINGPSIEKAGDLAAILTTL
                     EAGDVLFIDEIHRLPRVVEEVLYSAMEDFKLQVMITLDGNVKNLQVDLPPFTLVGATT
                     RAGDLSSPLRARFGISEKIDYYEESDIFNIIKRTSRVFELPINDDAALEIARRSRRTP
                     RIANRLFRRIRDFATFASKRVIEYELCLEALKHLRVDTLGLDEVDIAYLTTLVERFRG
                     GPVGLETLANAIGEEVTNLEDVYEPYLLQIGMIDKTPRGRVATEICYAHLKKPRQTHL
                     F"
     gene            11426..14035
                     /locus_tag="PLU08_02900"
     CDS             11426..14035
                     /locus_tag="PLU08_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70678.1"
                     /translation="MRRLTAYLTLTFTLIFGAALNMFSPLRNLVSNHEYQTGREFVYR
                     ISDKDPDKDLDPKAIDEIVTIMDRRMQNYGVSEYNIAKEGKNIVRVSTTLQSTEEYNR
                     LQVYLNYNANFTMKIGDDDNTEAILSADQIFADKKARVEYRGPYPFIVFPLSDPTLFE
                     TTIVDVAQKIQEEKGSQETAEGEDKPALVKEATIVLWSDYDPDKDSYMSPVGDTQGKI
                     FLSFDYRSMWWNEDKTEIGVASPLEGMSENHKYTTAQIKKASETARYMMNVFNAGTLD
                     YKVDFLYANKAMAPTVEPLINLGTRDNIALSATFFASLVTMVVVLVILFAIYRLPVLN
                     ALSSSLLTFLGTMIIFNAIQVEFSTSAIIGVLIVTFVSILSTILYAAKFRNEVYKGRT
                     FKKAHTEAITRSSFLTIDILVLLLVTGIFTYFFGGAALTGFATTLIFGSIVMVASVLI
                     HNSLLLPLLAHNRVTSKSYTYYGINESKVPDTLKDEKQTYFGRFVNHKPENRAKLSMI
                     LTGSLSLITAVLLIVFSAVNINPYNLVKHDENITRVYFQVSENSDINPLSPTASPVTL
                     LNYIELDGEALTFEKKEGAPVYETQVYKTMELDIEDEIEVTYNFYVYNLIGVYTGEEE
                     VNYKVTADAPMQSATLSEALDIIVKNIDDKAVISFNAVEKEAITPHFGRIAWVSLVIL
                     AVTSFYLTIRFGLTRGITNFVESFVAASLVFLFLMVTRLAIPPLATMGAFVAILAVSF
                     LNVVIFTNIKDVQEETASKGLTHREHFELGLRYSLSVIYITVLMFSLGFLVFTLIGPL
                     AVSSVYLASAVAMVAALLVVVKYSTAFLSGSLNVLDRLPKLERKQKTVTGKQRTSIRS
                     NEPEEAIFIGIND"
     gene            14086..15636
                     /locus_tag="PLU08_02905"
     CDS             14086..15636
                     /locus_tag="PLU08_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOQ70679.1"
                     /translation="MNKFLDKLLKYYDLSLRDYEKLTAEVTKENLPTAEAFFNFESFI
                     SRIEKAIASDENVVIYGDYDADGILATSILKYAFLKRGKDVFTMIPSRYKDGYGLNTN
                     VVEKFSKRAINLIITVDNGINQHDAIDLANELGIDVLVSDHHELSETLPNAIAILHPE
                     LSNLVRLNSCGAYMALVISYGMLGYYDDYLISLAAIATLADMMPLIDRNRTLVRLAIK
                     ATNDHQYFPLVKLNDSTFFDETSMSMKIAPKINALGRLSQNYESNVLVEYFTTTDVKR
                     HYDISDYIEKVYKKRKAMSRGEELSSEELKNPVIALITDELEGVLGLIAQNYMLEHNK
                     PAFIFTESSEDPSLLKGSVRSKSGFNVVDAFSHLEHLILTSGGHKEAGGLTIRKQDFI
                     VFKEALNKYAKNYPLITQDEKYLDVTLDEITKENYALIKNLSPFGHKFRAPLLRLTSL
                     TNVKYLFSRDGKHIITPLESGVKLIGFSLAEESKNALNIGFTATFAESEFRGRRSIDL
                     VIKEFFTA"
     gene            15723..>18786
                     /locus_tag="PLU08_02910"
     CDS             15723..>18786
                     /locus_tag="PLU08_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOQ70680.1"
                     /translation="MKRRKLLLLPVFLLLLAGCKDTSSSVSVEDSQPTVTSTSEVEPS
                     SEEPQVTSVEDTEDLPSVPTSEAPVFEHKTIEEIRAMTEDGASATPDQKQLFYETSGV
                     VTAKFRGANIDGVGPAWNIAIQDGEHALLLYAVTEEAYAADFADVPIGSKLTVTGNLA
                     AYKGLRELSTIKYVSHEAASPVEPEVLASIEQADLAGKDSKLVKIEGLKLHAKPQLEA
                     YVDGDRNRVNINMDLRKDGVKIGTYMHYNIPLADAQATVEKLQTVTSNSTVTWTGILG
                     MHEKVFQLTNANADEWEIEVGAPVVLETIAFRDETKELAPGATYQTTLVSTPFDAVVG
                     EVTYASDAAAVATVDAEGVVTAVAVGTANITATASGKVATMVITVADAAEGVYDITVV
                     DSYTTNSEIKTEEEFLAAVKGYPVGMTVPEVVKVYGIAPTMGSNQHNDYTATTGFKFS
                     SSKDNAALTLKMPAGVEIAKVKVEGRAWSNETATLAINEGEAQTFMVRDDADPNLLET
                     REFVLATPSETLSFVFTKRVIASKITLEVAGAVTPPEEIEGTIAHFLAQPLDYVGTLE
                     AVITNLGPYNSFGMEDATGAVAFRKSGINAANAPFAVGDKVSGSFKKVDYNGLMQAEL
                     QDAAPTVEAGPHALTLPRVNLNEVGLTAEALGPYQSRLVTGELLVKSPATVEGNGTIT
                     LPLTNGTNDISVRLDYRLPKIADFDYLKTLVAGDSVVLKGAVIGWYNGPQLAADDAAQ
                     LEVIRQMSIEAFLALEIGALGKLSGVITNMGPHNSFGMEDATAAVAFRKYNIDSTTTL
                     FAVGDKVSGTFKKTDFKGLIQAELQEDAPTVEAGPHALTLPRVNLNEVGLTAEALGPY
                     QSRLVTGELLVKSPATVEGNGTITLPLTNGTNDISVRLDYRLPKIADFDYLKTLVAGD
                     SVVLKGAVIGWYNGPQLAADDAAQLEVIRQMSIEAFLALEIGALGKLSGVITNMGPHN
                     SFGMEDATAAVAFRKYNIDSTTTLFAVGDKVSGTFKKADYKGLIQAELQ"
BASE COUNT         6002 a         3228 c         3780 g         5776 t
ORIGIN      
        1 atttaaaagt ttcctaatct ttatttgtaa agataagtat gtcatctaac aaatttaacc
       61 ttttcttcta ttagcataag taacttgttg caattaagtg cttaagagtg ataaaataaa
      121 caggcattgt cattacagaa gtaagattac gaggcgacac agagagtcga gcaaggtgaa
      181 agtcgacacg cagccactta ctgggtcata gtgactaaag atgtccgggt aatgtccgtt
      241 atcacatcta atgaagtgcg tatacatata tacgaaataa ggtggtacca cggagcaatt
      301 cgtccttaaa gtggtacttt tttatttatt ggagggaaat atgaaacttg atacccatta
      361 taaccatcta aaggtggaaa aaggtcgtta tgaacactgg aaaaaaagcg gctattttaa
      421 aagtggtgat acatctaaac caaaattcag tgtcgtgatc ccacccccga atgttactgg
      481 tgttttacac ttaggacatg cttgggacaa tacattccaa gatattattg ctcgctataa
      541 aaaagcgcaa gggtttgatg ttttatattt accaggaatg gatcacgctg gtattgctac
      601 acaagcgcgg attgatggac ggcttcggga acgcggctta tcacgttatg acttaggacg
      661 cgaaaaattc ttagaagaag catggaaatg gaaagaagaa tacgcgagta cgatccgtag
      721 tcaatgggca aagctcgggc tcatgcttga ttatgaccat gaacgtttta cgcttgataa
      781 gggactaaat gacgctgtta agcatacatt tgtggaactt tataaccgcg gttatctcta
      841 tcttggtgaa cggattatta attgggaccc cgttttccaa acagcgctta gtaatattga
      901 agtcattcat aaagatgttg aaggctcatt ttattacttt aaatataaac ttgaaggcag
      961 tgacttttat ttaagtgtcg cgacaacgcg accggaaaca atgtttgccg acgtcgccct
     1021 tgtgattaac ccacgcgacg agcgatacac aaaatactta ggtaaaaact tcgtaaatcc
     1081 agtaaataat gaattaattc cgttaatcgc tgatgattat gttgatttag aatttggtac
     1141 tggtgtgatg aaatgtacgc ccgcgcacga cgcaaatgac tttaaaatcg gtgaaagaca
     1201 taacttaagt cgcccgattt gtttaaataa agacgcaaca cttaacgaac gcggtcttga
     1261 actagcgggt ctagatcgct tcgatgcacg cgaaaaactc gtcgccaaga tgaaagaact
     1321 tgatttactt attaaagtgg agaaaatcgt gcataatgtc ggtcatagtg aacgaagtga
     1381 tgccattatt gaaccgtatt tatcaaaaca gtggttttta cgcatggatc actttgctaa
     1441 aaaaagtatc gaagggcaaa aaggtgatga ccccattcgc tttttcccga aacgctttaa
     1501 taaaacttat ttaaattgga tggagattgc tgaagattgg tgcgtatcac gccaaatttg
     1561 gtggggtcac cgtatcccgg cttattatca taaagaaact ggtgaaatag tagtaagtgt
     1621 tacaccacct aaagacttag aaaattatcg gcaagatgaa gatgtccttg atacatggtt
     1681 tagcagtgca ctttggcctt tttcaacgct agactggcca aatacggata gcgaactctt
     1741 taaacgctat tacccgaccg atgtaatggt gacaggctac gatattattt tcttctgggt
     1801 atcacggatg attttcatgg ggttagagtt aactaatgaa aaaccattta aagacgtcgt
     1861 tattcacggt ttaattcgtg atgaacaggg ccgaaaaatg tcaaaatcac tcggcaacgg
     1921 ggttgatcca attgaagtga ttgcgaagta tggtgttgat agcctccgtt atttcttagc
     1981 aggcgcaagt accccaggcc aagatgcgcg ctatagtgaa gcgcgtatcc aagcaagtgt
     2041 caattactta aataagattt ggaacgcagc taggttctta cttttaaact taccagaaga
     2101 ctttaaacca ggtaagatta atcataagaa actgcatcct gttgatgctt ttatcttaag
     2161 tcgttattac gaaacagtgc gtaaagtcac aagtttaatg gataagtatg agcttggtat
     2221 ggctagtaat tatctctatg cttttgttta tgatgattac tgctcaaatt atattgaaat
     2281 ggttaaagtt attttaaata gtgatggtga tcatacaaat acatataatg ttatgtatca
     2341 cactttacac ggaatcttaa tgatgattta tccttattca ccttttatta gtgaagaaat
     2401 ttatttagcg ctcccaaatc atctcgattc tataatgtta gcgagttatc caaaagctag
     2461 tcgtttacgc tttaagcgcg aagcggaaga agtaaatacg attattaact taattaaaga
     2521 aattcgtaac tttaagagca ctaataactt agcgcccaat actaaaatga atttatatgt
     2581 cgctgatgtt agctttgatt taacgcctta tcttgatatt attcgccgtt taggttttat
     2641 taatgaattt aagtatagtg atgttcctat tgaagtaacg acagttagtc ttccaaaagc
     2701 tacttttggc ttagagttag atgttgatcg cagtgtgctg attagtcaac ttgaaaaaga
     2761 aatcgaaact ttaacgaaag aagttgaaag gagcactaag cttttaaata accctggttt
     2821 taaaaataaa gctcccgcaa gcaaagttgc ggaagaagaa gaaaaagccc gccattacgc
     2881 tgaaagtctg gcgctcgcgg ttgataaact aactaaattg cgataaaaca ttaactttcc
     2941 ttttacttaa tagtaggagg aaattattat gactttatta actaatgccg aaatggcaaa
     3001 tattaaaggt ggtgaaccaa tcactttagc agcggtaatg acaattttag tcatcgctat
     3061 tgtaacggtg attgtttata aattatttac ttcaaacgcc ggttctacga caataccggg
     3121 aggttttaaa tttgaatgga agtaaacgca ttaaggagtt accccattta aaaagaccgc
     3181 gtgaaaaggc gctttattac gggattggta gtttaagtga cgccgaagta cttgcgctgt
     3241 taattgggaa aggcacaacg aaagataatg ccttaagtct tgctaagaag cttttaaata
     3301 aacatatatc cttacaaaat atgtatcgga ttgatgaccc gaagctcctt ttaatgccag
     3361 gaattggtga agttaaggca ctgcaaattt tagcggcatt tgaattaaat aaaaggcttt
     3421 tgtttcttga aggaagtcac gaccgcccaa tatatcgcga agacgaaatt gtgcgttcct
     3481 ttcaaaggcg gattggacgc ttacaaaaag aaacgctcta ccttttagcg ctcacgaatc
     3541 gtaattatat tattagtgaa aaacaactat atttagggac ggaaaaaggc tttagccttg
     3601 accctaaaga agtagtaaga gaattattaa tcgctaacgc tgatcgttat attttgattc
     3661 ataatcatcc tagtggtcat gttgaaccta gcgcaaatga cttagaaaca acgatcgagt
     3721 tagcaacaat aagtgcccaa tttggtgtta ctctttatga ccatttaatt attggtgcaa
     3781 atgcttattt ttcattaaga aagcttggta agttgtaaaa tggtattatt ttagtagttg
     3841 tttagtagat aataagtttt agcattatta tttatatctc tatactatta tgtctgcgat
     3901 tgtttttaat atctcaattg tatcaaagaa tttaatatcg gctgcataac tatttttttg
     3961 catgaacttt tcccactcat taattagctt actattgttt tgcatattat ttaatatttc
     4021 tttgctatcc ttaataatct ttgttgtctt tctcttgttg gaagtattaa aaatagctct
     4081 ttttaaagtt ttaaaattaa ttgcattatg tttaagttta aaaatcatat aaacatcgta
     4141 aaaatctttc attcttgttg tttctatatt gcgaacaatt attgtttcat atttttctgc
     4201 gatgattgtt tcaatattat atgcatataa cctgattttt tctgatgtta agagacacga
     4261 atattcatat actattttgc ttggtgtaat tggatcacca gttgttatat ctagtttaag
     4321 aggaattcta gtattttcta acattgctac aagcgatata cgaaagttat catagtaatc
     4381 gatttcccta ataatttcga tgtttgtaaa aataaattct acgccatcat ttatgtctat
     4441 attgataatt tctttgatga cattagttat catttcttgt tctaaattca tgtctttaat
     4501 atttgtatct atatcaatag ttgtcctatt agtgattccg ataattgaag atattaaaaa
     4561 cccaccttta ataatgaatt tgtctttata gagtgatttt gaaagccgca tcaaaaacct
     4621 ctcaaagaag tacatcctta aaattatatt tgcacttaaa ccgatttgtg aagcaagatt
     4681 tcgtgtttta gctttaatac ttgtaatatt aatcatgaaa gaatctcaat atagcgtaaa
     4741 atttcatttt caatcttaaa ttgacgacta tatttgatta actttttgat atctttatct
     4801 tttcgacgaa aatatcttgt aattgcatca acaaaaactt ctttttcgat attttctcga
     4861 tcagcgatga tatcacaaat ggttctttcc atatcataaa ctaaaatttt gttgccaaaa
     4921 tatgttttca tcgtaataat accgatttta tgatgttttc ttttgacata tcttgttttt
     4981 aaatttatat atcttttctt aatatgtgaa gtgttatatc cttgtggtac tgtgatgtag
     5041 agtctatctg gcattcgatc tgataaatca tggagaataa gagcggtctc atgcgaaaaa
     5101 caaacctgat tactattagc agaaactaac atgaaatcat caataacttc gccggccttt
     5161 tgatagacac caggtcttag tctctcaagt tttccctctt tcactaatac tgaaagtagt
     5221 tggcgattaa caccaattct agcagcgtct ttattagtga ttactggcct ttcatttaag
     5281 tagttttgta aacttgtcat agcatccttt cttacacttt cgctttaatt atacgaaatt
     5341 aaagagtaaa tgtaaatata tgtaagaagc acttccacat tacttaatag tcaagaggta
     5401 gtgctttctc aatataaaat acaatcgaaa taagtatttt tactacaaat aaatgattta
     5461 tatgacagtt taatgtgaaa aaatcattgt tttatatttt aacccttgcc taaacctatg
     5521 gtttatgata ttttacatat gttatcgcct cacaagcttt aaccgcctag aaatggttag
     5581 tgtgcaaaga agaacaatta acttctacca taatagcgag tccaagtgga aatatataaa
     5641 ggaggttagc acatgtacgc aattattgaa acaggtggca aacaagttaa agtagccact
     5701 ggtgatgtaa tttatgtcga aaaactcgat gttgctgaag gagaagattt cgtctttgat
     5761 aaagtcttac tcgtcggtgg taaaaaagct aaagttggaa ccccatatgt taaaggtgcc
     5821 accgttagcg caaaagtagt gaaacacggc ttaaatcgca aaattaaagt attcacctac
     5881 aagaacaaaa gtaatgttag acgcatgctt ggacatcgtc aaccatatac ttgccttgaa
     5941 gttgtggaaa ttaaaggtta agtatgatta atgtcgttat tactacaatt gatgaagaaa
     6001 tcttaaagat tcgcattaaa ggtcacgctt atagcggaga accaggtcat gatctcgtgt
     6061 gtgctggtgt aagtgccgta agtattggtg cacttaatag cttacatgaa gttgatactg
     6121 ccttcaaagt tactgttgaa gatggtctag tggaaatagt cccattatat agaccaagtt
     6181 acgaaaacaa gattgtgctg caagtattaa taacgagctt aaaaactatt gcagaatcat
     6241 acggaaagta tgtacaaatc cgtgaagaaa ggagagcaga agcatgaaat tcattctaaa
     6301 cttacaatta tttgcctcga aaaaaggtgt tggatcaacg cgcaacggcc gtgactccac
     6361 tggtcgtcgc ttaggtgcta aacgcgctga tggtcaacta gcgaaagctg gggcaatttt
     6421 atatcgccaa agaggtactc ggatttatcc aggacttaac gccagaagag gtagtgatga
     6481 tacaatctac gcgactgttg aaggtattgt ccgttttgaa cgctttggta aaacacgcaa
     6541 acgtgttagc gtttatcctg ttaactaatt agagaaacaa aatgaccacc gctcacttaa
     6601 gtcggtggtc tttttttcat atataatata aaatatatta ttgaaaagag gtacacatgg
     6661 ctaaaaaatt agtgacggtt gtcgtcccaa tgtataacga agaaccgatg gttccccatt
     6721 ttttaacaaa aatcgccgaa attattaaag aaaatgatgg ctacaacttt gaagtagtag
     6781 cggttaacga cgggagtcgt gataatacgc tggccttact ttatgatttt cagaaaaaat
     6841 ataaattttt acgtgtcgta aatttaagtc gtaactttgg ccacgaagca gcagtggcgg
     6901 caggactaag tgttgccaag ggtgacgcta ttattccaat tgacgctgat ttacaagatc
     6961 caccggagct tattaaaccg atgcttgaac tttttgaaga aggatatgat gtcgttaatg
     7021 cgcgacgtgc ttcgcgtaaa gaagatacgt ggattaagcg ttacactgct ggtaaatttt
     7081 acagaataat tgataagtta tcagggaaag tacggattcc gcaaaatgtt ggtcattatc
     7141 ggcttatatc aagacgcgtg cttgatgaag ttttaaaatt accggaaagc aatcgcgtct
     7201 ttagagtaga agttccttat gttggcttta aaacagcgga agtagcgttt gttagaccca
     7261 aaagagaaca tggtaaaacg cattacaata tgaagtcaat gattgattta gcaattaacg
     7321 ctattacaat tacgagtact aaaccactta actttgtctt taagtttgca atgtggctgg
     7381 ggattatctt tggtttaagt gccgtgacgc aacttgtgct atttatttta gaaatgacta
     7441 aggtattagt gctgcctttt cttaatcacg gtgtgtggtt attgataaat gttagtttat
     7501 ttttaacaac agttatcctt ttagcgctta caataatctc aatttatctt ggcaaaacat
     7561 ttaatgaaac gcaaaaacgc ccattttatg ttattgaatc agtaaaggaa aataaataaa
     7621 gaatgtttat cgaccaagct aaaataacac ttaaagcggg taatggcggt aatgggatgg
     7681 tctcttttcg ccgtgaaaag tatgtgccaa aaggggggcc agatggcggg aatgggggtc
     7741 gcggcggttc catttacttt gtcgctacaa gtaatatttc aacacttact gattttcgct
     7801 ttggtttaac gattaaggct aataacggcg gtaatggcgc aactaaaaat cagtacggta
     7861 aaagtggtga agatctctat gtcaaggtac caatcggcac tgttgtttat cttgaaccaa
     7921 gccgcgaagt aattgccgac tttaaacttg agggcgaaac aatgttaatc gctaaaggtg
     7981 ggaaaggtgg tcgcgggaat gctgctttta aaagtagcac gaatcgcgtg ccgcgtattg
     8041 ctgagcacgg tgtacctggt gaagaactac ttgtgacact tgaattaaaa ttacttgcgg
     8101 atattggttt tattggctta cctaacgctg gtaaatcaac acttttaacc gtgatcagtg
     8161 aagcacgacc acaaattgat aattatcctt ttacaacact tagaccagaa ctaggagttg
     8221 taaaagctaa tagcggtaac cgttttgtgg ctgcggactt accaggcatt attaaagatg
     8281 cacatttagg acgcggactt ggcttacgct ttttacggca tgcgcagcgt tgtaaggtgc
     8341 tcgctttcat cattgacggt gaaaatagtg aagattacta taaagaatat aagttactgc
     8401 gtaatgaagt gaaggaatat gggtataaat tagatgaaag acccttcttt gtggctgtaa
     8461 gtaaagatga aacagtagat actcataaaa aagtaaaagc actagcgaaa aaactaaaag
     8521 atattaaagt gcttggtttt agtagtatct tacatgaaaa tatcgatact ttaattaata
     8581 cgatggaaga atatattgtt aacgctcccg aaattgaaat ggttgatgaa gaaggcgttg
     8641 cggttaagta tcgagagtac gacttaacgg cgactattga ccctgattta tttacgattg
     8701 agaaagttag agccggagtg taccgtattg ttggggaaaa agttgaggaa agatatcata
     8761 aatacatctt aaatacagaa gaagcgattt tatcactttt aaattatctc cgtgaaattg
     8821 gtgtcgaaga tgcccttgat aaaatgggag ttaatgatgg tgatactgtg attctcgttg
     8881 actttgaatt tgagtatttc cgctagggag gaattatggg acaagaaagt ttacaaaatt
     8941 atttacagta cttagtgcgt tacttaaagc gtagagcacg cggatttaat ggttatattt
     9001 tagggttaag tggtggtctc gatagtgcag ttgtggcact tcttgcccaa aaagccgtaa
     9061 aggacgactt attatgcgtc attatcgata ttgaaagcga aaagtgtgat atggatgatg
     9121 ccttagaact ttgtaaacta cataatttaa attactatta cattgattta actgcagaat
     9181 accgcgcggt tgtgaaaaaa cttgaagaaa gtaaaaagtt aaccgaactt agtaaaatta
     9241 atacgaaagt tcgtttacgt atggtgacgc tttatgcttt agggcaaaca catagcaaat
     9301 tagtgctcgg cactgataat aaggcggaac tctttactgg ttactttaca aagcacgggg
     9361 atggggctgc tgatttattc gtcctttcaa gcttaacaaa gggtgaagtc tttgccgcag
     9421 cgcgcttact tggtgttacg ccaaacatta ttaataaaat tccaagtgct ggactttatg
     9481 tcggacagac tgacgaagcc gagatgggca ttaaatacga agatcttgac gcctttatca
     9541 gcggtaaaga agtatcgcca agcgttaaag cacgcgcaat agaactgcac cgcatcagca
     9601 atcacaaacg acgtccaatc cctcgaccac gggagaaaag atagtgaaaa agcaaaaatt
     9661 tgtgagtaac acgattatta gtggcctagc tgccgtactt ttattcgtcg tgttacttat
     9721 atttataagg atggtgggag tatgatttat ggattaaaag gcattgttac aaaacaaacg
     9781 gcgacaacga ttgtcttaga tgtgcgtggt gtctattacg aagttaaagt gccgtccgtg
     9841 ccacagcaac aacagtttag tgaactattt ttatatacat atcaagtaat tagagaagat
     9901 gagcattatc ttgttggctt tttaagtctt gaagaaaaaa gcttatttac cgagctttta
     9961 aatgtttcag gcattggtcc aaagactgct ttagggatca tgacggccac aacaccagag
    10021 cgcttcttac aggcagtcac gagtgaagaa cttagttact taaagaaatt accgggcgtt
    10081 ggtccaaaag gcgcgagtca aattattctc gacttaaagg gtaaattaca aactgacact
    10141 tcaaaaggct taaatcctta tgaacaagaa gtaacgcttg gattacaaag cttagggttt
    10201 aagaaaagtg aaattactaa agttttaaag aaagttagta ataagtcgct tgacgctaat
    10261 gagttattac gtctagcgct tagtgagtta agacggtaat gagtaacaga ttagtgagtg
    10321 ataaattaga tcaaagtgaa gcaagtgtgg aattaagtct ccgcccgtta acattgaatg
    10381 aatatgtcgg gcaaactgcg cttaaacata atttaaatat ttttatcact gccgctaaag
    10441 cacggaatga agtacttgat catactttac tttttggacc gccaggactt ggaaaaacaa
    10501 cattagcgca cattatcgct aatgaaatgg gcgcaaatat taaagtaatc aatggtccaa
    10561 gtattgaaaa agccggtgac ttagcagcga ttttaacgac tttagaagca ggtgatgtgt
    10621 tatttatcga tgaaattcac cgtttaccgc gtgtcgttga agaagtctta tatagtgcaa
    10681 tggaagactt taagttacaa gtgatgatta cgcttgatgg taatgttaaa aatttacaag
    10741 ttgatttacc accctttacg cttgttggtg caacaacaag agccggtgac ttaagtagtc
    10801 cgctccgggc acgctttggc attagtgaaa aaattgatta ttacgaagaa agtgatattt
    10861 ttaacattat taaacggaca agtcgtgtgt ttgaattacc gattaatgat gacgctgcgc
    10921 ttgaaattgc acgcagaagc agacgaacac cgcgcattgc taatcggctc tttcgccgca
    10981 ttcgtgactt tgcgactttc gctagtaagc gagtgattga atacgaacta tgtcttgaag
    11041 ctttaaaaca tttacgtgtt gatacgcttg ggctagatga agttgatatt gcttatttaa
    11101 caacactagt agaacgcttt cgtggtggac cagttgggct tgaaacccta gcgaacgcga
    11161 ttggtgaaga agtgacgaac ttagaagatg tttatgaacc atatctctta caaattggga
    11221 tgattgataa aacaccgcgt ggtcgtgtgg ccacagaaat ttgttatgca caccttaaaa
    11281 aaccacgaca aacacattta ttttaaaaaa atgccgatat tttcactaat tagcgttatt
    11341 ttggttgtga aaaaagtcat ttaatagtaa tctattaaag cgattatgtg tatcgaagta
    11401 tttttagtat gacggaggaa ttcttatgcg tagactaaca gcatatctaa cattaacatt
    11461 tacactgatt tttggagccg cgctaaatat gtttagcccg ctccgtaatt tagtgagtaa
    11521 ccacgaatac caaactggtc gtgagtttgt ttatcggatt agtgataaag atcccgataa
    11581 agatttagat ccaaaagcaa ttgatgagat tgtgacgatt atggaccgcc ggatgcaaaa
    11641 ttatggcgtt agtgaataca atattgctaa agaaggcaaa aacattgtcc gtgtctccac
    11701 aaccttacaa tcaaccgaag aatataaccg tcttcaagtt tatcttaact ataacgctaa
    11761 tttcacgatg aaaattggtg atgatgataa tacggaagcg attttaagcg ctgatcaaat
    11821 ctttgccgat aaaaaagccc gtgttgaata tcgtggtcct tatcctttta ttgtcttccc
    11881 gttaagtgac cccactttat ttgaaacgac aattgttgat gtcgcgcaaa aaattcaaga
    11941 agaaaaaggt tcacaagaaa cagcagaagg cgaagataag ccagctttag tcaaagaagc
    12001 aacaatcgtc ttatggagtg attatgaccc tgataaagat agttatatga gcccagttgg
    12061 cgatacacaa ggtaaaattt tcctctcctt tgactatcgc tcgatgtggt ggaatgaaga
    12121 caagaccgaa atcggtgtcg ctagtccgct tgaagggatg tcggaaaacc ataaatatac
    12181 aacggctcaa attaaaaaag ctagtgaaac cgcccgttat atgatgaatg tctttaacgc
    12241 tggtacactt gattataaag ttgatttctt atatgccaat aaagcaatgg cgccaacggt
    12301 tgaaccactt attaatttag gtacacgcga taatattgcc ttaagtgcaa cattctttgc
    12361 tagtttagta acaatggttg tcgtccttgt tattttattt gcgatttacc gtttaccagt
    12421 cttaaatgca ctaagtagta gtttattaac attcttaggc acaatgatta ttttcaacgc
    12481 gattcaagtc gaatttagta caagtgccat tattggtgta ctgattgtga cctttgtaag
    12541 cattttaagt acgattttat acgctgctaa gtttagaaat gaagtgtata aaggccgcac
    12601 ctttaaaaag gcccacactg aagctattac ccgttcaagt ttcttaacaa ttgatatttt
    12661 agtgttatta ctcgtcacgg gtatctttac atatttcttt ggtggtgcag cactcactgg
    12721 gtttgctaca accttaatct ttggtagtat cgtgatggtg gcaagtgttt taatccataa
    12781 cagtttactc ttaccacttt tagcccataa ccgtgtaact agtaaaagtt atacttatta
    12841 cggcattaat gaaagcaaag tacctgatac tttaaaagat gaaaaacaaa catactttgg
    12901 gcgttttgtt aatcataaac ccgaaaatcg cgcgaagctt agcatgattt taactggtag
    12961 tttaagcctt attactgctg ttttacttat cgtcttcagc gctgttaata ttaacccata
    13021 caacttagtg aaacacgacg aaaatattac gcgtgtttac ttccaagtca gtgaaaatag
    13081 tgatattaac ccactttcac caaccgcaag tcctgttact ttacttaatt atattgagtt
    13141 agacggagaa gccttaacct ttgaaaagaa agaaggtgcg ccagtttacg aaacgcaagt
    13201 ttataaaaca atggaacttg atattgaaga cgaaattgaa gttacttaca atttctatgt
    13261 ttataactta attggtgttt acactggtga agaagaagtt aactataaag tgactgctga
    13321 cgcgccaatg caaagtgcaa cgttaagtga agcgcttgat attattgtta aaaatattga
    13381 cgataaagcc gttatttcct ttaacgctgt cgaaaaagaa gcaattacac cgcactttgg
    13441 ccgcattgct tgggttagtt tagtaatcct tgctgttact tctttctact taacaattcg
    13501 ctttggactt acgcgcggta ttacaaactt tgttgaaagc tttgtcgctg ctagtttggt
    13561 gttcctattc ttaatggtaa cgcgtctagc aatcccgcca ctagcgacaa tgggagcctt
    13621 cgttgcgatt ttagccgtta gtttcttaaa tgttgtgatc tttacaaaca tcaaagatgt
    13681 ccaagaagaa accgctagta aagggcttac gcaccgtgaa cacttcgaac ttggacttcg
    13741 ttatagttta agtgtcattt atattactgt cttaatgttt agtcttgggt tcttagtctt
    13801 taccctgatt ggtccgcttg ctgttagcag tgtctacctt gctagtgctg tagcgatggt
    13861 tgcggcactc ctcgtcgttg ttaaatatag tacggccttc ttaagcggtt cgttaaatgt
    13921 ccttgatcgc ttaccaaaac ttgaacgtaa acaaaaaaca gtcactggta aacaacggac
    13981 aagtattaga agtaatgaac cagaagaagc catctttatt ggcattaatg actaatatta
    14041 attaaataaa tacaaaaagt actacccgtg tggtagtatt tttttatgaa taaattcctc
    14101 gataaattat taaagtatta cgacttatca ctaagagatt acgaaaaatt aacggcagaa
    14161 gtcactaaag aaaatttacc aacggccgaa gcttttttca actttgaatc ttttattagt
    14221 cggattgaaa aagcgatcgc tagtgatgaa aatgtcgtta tttatggtga ttatgatgct
    14281 gacggcattc ttgctacgag tattttaaaa tatgcctttc ttaaacgtgg taaagacgtt
    14341 tttacgatga ttcccagccg ttacaaagac gggtacgggt taaacactaa tgtggtcgaa
    14401 aagtttagta aacgcgctat taatttaatt attaccgttg ataacggcat taatcagcat
    14461 gacgcaattg atctcgctaa tgagctcggt attgatgtac ttgtgagtga tcaccatgag
    14521 ttaagtgaaa cgctcccgaa tgctattgcg attttgcatc ctgaactaag taatttggta
    14581 cgtcttaata gttgtggcgc gtatatggcg ctagttataa gttacgggat gctaggatat
    14641 tatgatgatt atttaattag tttagcggcg attgcaacac tggcggatat gatgccacta
    14701 atagaccgta atcgtactct tgtccgtcta gcgataaagg ccactaatga ccaccaatat
    14761 ttcccgcttg ttaaattaaa tgacagtacc ttttttgatg aaacaagtat gagtatgaaa
    14821 atagcgccaa aaattaatgc attagggcgc ttaagtcaaa attatgaaag taatgtgtta
    14881 gttgaatact ttacgacgac tgatgttaaa cgccattatg atataagtga ctacattgaa
    14941 aaagtatata agaagcgtaa agcgatgagt cgcggagaag aattaagcag tgaagagtta
    15001 aaaaatccag taattgcttt aattactgat gaattagaag gcgtacttgg acttattgcc
    15061 caaaattata tgcttgaaca taataaacca gcctttatct ttaccgaaag ttcagaagac
    15121 ccttctttac ttaaaggtag cgtaagaagt aaaagcggtt ttaatgttgt tgatgctttt
    15181 agtcatttgg aacatttaat actcacaagt ggtggccaca aagaagctgg gggcttaacg
    15241 attagaaaac aagattttat agtttttaaa gaagcactga ataaatacgc gaaaaattat
    15301 ccgcttataa cgcaagacga aaaatattta gatgtcacgc ttgacgaaat tacaaaagaa
    15361 aattatgcac tcattaaaaa tctttcacca tttggtcata agtttagagc gccgctattg
    15421 cgcctcactt cccttacaaa tgtaaaatac ttgttttcgc gtgatgggaa acacattatt
    15481 accccacttg aaagcggtgt taaactaatt ggcttctctt tagcggaaga aagtaaaaat
    15541 gcacttaaca tcggctttac tgctactttt gccgaaagtg aattccgtgg ccgaagaagt
    15601 attgatttag ttatcaaaga atttttcact gcctaaatag taataattaa caagtaatta
    15661 ttttttgtcc tttttttttg attatgttat gattaatatg tagtaattaa aggaggaaaa
    15721 taatgaaaag aagaaaatta cttttactac cagttttctt gctgctacta gccggatgta
    15781 aagatacatc atctagtgtc agtgttgaag actcccaacc cactgtcaca agtacaagtg
    15841 aggttgaacc aagtagtgaa gagccacaag ttacttcagt tgaagatacc gaagatctac
    15901 caagtgttcc tactagtgaa gcaccagtat ttgaacacaa aacaattgaa gaaattcggg
    15961 caatgaccga agacggtgct tccgcaacac ctgatcaaaa gcaattgttc tacgaaactt
    16021 caggtgtcgt caccgcaaaa ttccgcggtg ctaacatcga tggtgtcgga ccagcttgga
    16081 acattgcaat tcaagatggt gaacacgcat tattactata tgctgtaacc gaagaagctt
    16141 acgctgcgga ctttgctgat gtaccaattg gttcaaaatt aactgttact ggtaacttag
    16201 ccgcctataa aggccttcgt gaacttagca ccattaaata tgttagtcat gaagctgcat
    16261 ctccagtaga accagaagtc ctcgcaagca tcgaacaagc tgacttagcc ggaaaagact
    16321 ccaaattagt taaaattgaa ggacttaaac tccacgctaa accacaatta gaagcttatg
    16381 ttgatggcga ccgtaaccgt gttaatatta atatggactt acggaaagat ggtgtcaaaa
    16441 ttggaacata tatgcactat aatattccac ttgctgatgc ccaagccact gtcgaaaaat
    16501 tacaaaccgt gacaagtaat agtactgtta cttggactgg tattcttggt atgcacgaaa
    16561 aggttttcca actcacaaac gcaaatgccg atgaatggga aatcgaagtt ggcgcaccag
    16621 ttgtgcttga aaccattgct ttcagagatg aaacaaaaga acttgcacca ggcgcaacat
    16681 atcaaacaac attagtgtca acaccatttg acgcagtcgt tggtgaagtt acttacgcta
    16741 gtgatgccgc agcagttgct actgttgacg ctgaaggtgt cgtaactgcc gttgctgttg
    16801 gtactgcaaa cattaccgct accgctagtg gtaaagtagc gacaatggtt attactgttg
    16861 ctgacgctgc tgaaggagtt tacgatatta ctgtcgttga ttcatatacc acaaattccg
    16921 aaattaaaac cgaagaagaa ttcctcgccg cggttaaagg ttatccagtt ggcatgactg
    16981 taccagaagt tgttaaagtt tatggtattg ctccaacaat gggttctaat caacataatg
    17041 attacaccgc tacgactgga tttaaattca gtagtagtaa agataatgct gctttaacct
    17101 taaagatgcc tgctggtgtc gaaattgcta aagtaaaagt tgaaggtcgt gcctggagta
    17161 atgagactgc aacacttgca attaacgaag gtgaagcaca aacattcatg gttcgtgatg
    17221 atgctgatcc aaacttactt gaaacacgcg aattcgtttt agctacacca agtgaaacct
    17281 tatcatttgt gtttactaaa cgtgttatcg cttcaaaaat tacgcttgaa gtcgctggcg
    17341 cagttactcc tccagaagaa attgaaggaa caattgccca cttcttagcg caaccacttg
    17401 attatgttgg tactttagaa gcagttatta ctaacttagg tccatataac tcattcggga
    17461 tggaagatgc aactggtgcc gttgcgttcc gcaaatctgg cattaacgct gcaaatgcac
    17521 catttgccgt tggtgacaaa gttagcggct cctttaaaaa ggttgattat aatggcttaa
    17581 tgcaagctga actccaagac gctgcaccaa cagtcgaagc cgggccacat gctttaactt
    17641 taccacgcgt taacttaaat gaagttggtc ttaccgctga agctttagga ccatatcaat
    17701 cacggttagt tactggtgaa ttattagtga agagccccgc aactgtcgaa ggtaacggaa
    17761 caattacgct tccattaaca aatggcacaa acgacattag tgtccgtctt gattatcgtt
    17821 taccaaaaat tgctgatttt gattacttaa aaaccttagt cgctggtgat agcgttgtct
    17881 taaaaggcgc tgttattggt tggtataatg gtccacaatt agccgctgat gatgctgcac
    17941 aactcgaagt cattcgtcaa atgtccattg aagcattctt agcgttagaa attggtgcac
    18001 ttggtaaatt aagtggtgtt atcacgaata tgggtccaca taactcattc ggtatggaag
    18061 atgcgactgc tgcagttgcc ttccgtaaat ataacatcga ctccacaacg actttatttg
    18121 ccgttggtga taaagttagt ggcaccttta aaaagactga tttcaaaggc ttaatccaag
    18181 ctgaacttca agaagatgca ccaacagtcg aagccgggcc acatgcttta actttaccac
    18241 gcgttaactt aaatgaagtt ggtcttaccg ctgaagcttt aggaccatat caatcacggt
    18301 tagttactgg tgaattatta gtgaagagcc ccgcaactgt cgaaggtaac ggaacaatta
    18361 cgcttccatt aacaaatggc acaaacgaca ttagtgtccg tcttgattat cgtttaccaa
    18421 aaattgctga ttttgattac ttaaaaacct tagtcgctgg tgatagcgtt gtcttaaaag
    18481 gcgctgttat tggttggtat aatggtccac aattagccgc tgatgatgct gcacaactcg
    18541 aagtcattcg tcaaatgtcc attgaagcat tcttagcgtt agaaattggt gcacttggta
    18601 aattaagtgg tgttatcacg aatatgggtc cacataactc attcggtatg gaagatgcga
    18661 ctgctgcagt tgccttccgt aaatataaca tcgactccac aacgacttta tttgccgttg
    18721 gtgataaagt tagtggcacc tttaaaaagg ctgattacaa aggcttaatc caagctgaac
    18781 ttcaag
//
LOCUS       DAOSTA010000019        15935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_3778_length_15935_cov_8.384131, whole genome shotgun sequence.
ACCESSION   DAOSTA010000019 DAOSTA010000000
VERSION     DAOSTA010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 15935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15935
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3778_length_15935_cov_8.384131"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..2125
                     /locus_tag="PLU08_02915"
     CDS             326..2125
                     /locus_tag="PLU08_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70681.1"
                     /translation="MKIKTRLDFIVIDGTELVVNEALKTLAKLPVSDLVEFLKGPKLY
                     SRRDHANILKNVMRDEVWKFKLVSRVAEKGLSNDFFSRLDYFEDSPVTVLEGLFERMV
                     KIQPELMDYYLEDLWLFAIQVVSKDKELAKLYKKAQKSSTSPVVLKELFPTLEGLFGD
                     EEGDIDGVLARTFVEKSLHSATIKEIREIAEKNDVLLPTNITKQDIVDGIIAVLPKVA
                     KAKDVPHLTEDVKDMTVAQLKEFVNKHKLDVPTELKKQDVGELLVKNYVNKNAANVVS
                     DLQYELPERVEEATDFSDENPKVIRLRERVKELEKELEERPVENETKVDESELLKEQE
                     KVEALSKELKQTKAELSEKPKEVVVEVPGPTVIRTVISKKELQKQKEHYEAIIAEQEK
                     ERSMLISRTEYDEVLAEYGRVKALLDEKDIEKLEEKPVEPKPAPQQVPQYEEPYYGSL
                     HAKVDRLTDKVHELQLEILRKELANQKATPAPQEEQPETEVNIYFDSNLAKRMSDNKN
                     AIDVGKAVKGKKRKKKSVGRTIFWIIFILLLLAIVVFMGAWTLEHFNITPLAGPPWLA
                     DRFNAAVLWLHNAVQSVFDLFKGLFGKVRVLGR"
     gene            2144..2599
                     /locus_tag="PLU08_02920"
     CDS             2144..2599
                     /locus_tag="PLU08_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70682.1"
                     /translation="MSKKRRIKELEAQVAALEAKVEGKEGQQTPKKAAKNSVNVYFDS
                     NFVNSLVSNQMKYGRRSRRDDLNVDPADKKAVRRARARRFFRFLWRLIVVLVIAALVA
                     AVVAAILMVIVWALVNFNIMTPTSNPFVGMVWEWIKWFFALFGMEVPIF"
     gene            2763..4022
                     /locus_tag="PLU08_02925"
     CDS             2763..4022
                     /locus_tag="PLU08_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOQ70683.1"
                     /translation="MSKLGDRIFQKVLRTFSPILPWREPEILKAYDELVDVLIKQNKS
                     RIFIVMDEGLVQLKLHQPLLDALKNKQLNYRVFSDITANPTTAQVEEGLLAYIIYGGD
                     AIIGFGGGSPMDVAKAIAARVARPRKNLKQLGGLLKVRKKTPLLIAVPTTSGTGSEAT
                     VASIVVDERTKHKYAINDPVLIPSYALLVPTLTFKLPKFVTATTGMDALTHAIEAYTN
                     KGGRKFPNEKAINATRLVFENLKVAYDNPSDLEARKNMQLAAYDAGVAFTRNYVGYVH
                     ALSHPLSAYYGLAHGYVNALILPFMLEKYGRRIYRKLARLARLSGVVEEGLSNGETAK
                     LFIAKIRALNTYFEIPTTIPEIKEEDIPQLAVYANKEARPLYPTPRIYKTSELEEFYR
                     ELKGEIFNVETNKNAKRSNEKKLSLKL"
     gene            3961..5304
                     /locus_tag="PLU08_02930"
     CDS             3961..5304
                     /locus_tag="PLU08_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOQ70684.1"
                     /translation="MLKQIKTQREAMKKNFPLSYEKRRELLINLQKAVKLYEKDIYEA
                     LYKDLHKDEWEVFVTELGIVYEEMHFAIKNLKKWMRRKKIKSFLVQFPAKNFLVPEPY
                     GQVLIMSPWNYPLQLTLLPLIGAIAAGNYAVVKPSAYAPEIAKVMQQIVATAFPNKEA
                     ILFLGGRKVNEEILQGVYDFIFFTGSPGVGQTVMRKASENLTPVVLELGGKSPLIIAE
                     DADIPLTIRRLKFAKLINCGQTCVAPDYVLLDKKLIDKVDDIRQAFSVEGEEKARLPK
                     IINEKHYLRLKGVLTEVGSDNFDEETQTINPTVVLNPPLDSELMREEIFGPILPIITY
                     ETLDEAIAFINERPKPLALYLFTKSKEIMNKVLRETTSGSVAINDAVVQLANPHFPFG
                     GVGNSGMGSYHGYHTFKTFSHMKAVLHKSRAFDFRFRYKPGPKTVGIFKKLTRNK"
     gene            5414..5887
                     /locus_tag="PLU08_02935"
     CDS             5414..5887
                     /locus_tag="PLU08_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70685.1"
                     /translation="MKLFQTVGRRKIPGYKSMSLERELVVLDPKKVEYVYFPLVAANN
                     VPLEVFVKEGDKVKVGTKIALKQQFSVPLFSSVSGTVRAIEKRLNVTTGRPCDHLVIK
                     NDFKMTKEKPLKKVELDASKEDIVAAIKEAVLSVLVALIFQHGLNTTMSKIFTLS"
     gene            5872..6714
                     /locus_tag="PLU08_02940"
     CDS             5872..6714
                     /locus_tag="PLU08_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOQ70686.1"
                     /translation="MHTILINAVECEPYLTTDYVSCSLNAAKVISGARYLQKAAGASK
                     VIVAIKVKKEQACNCLKEAAAPYPDIEVREVPDRYPMGWERTLIQTIFKKQYDKLPSE
                     IGVIVNNSTTAIAVHDALVEGSPIVSRIVTVSGNAVKNAANIVVPVGILAETVLNEFE
                     VEEKEVVLLVGGPMTSNPAKDVSFALTVCHGALTLLEKVVYRPVACLRCGSCTAHCPA
                     GLQPIEILRAFEGRDYERLEKLETLRCVDCGLCSYVCPSKIEVTDMMKKAKTLLRVHQ
                     SRKK"
     gene            6726..8564
                     /locus_tag="PLU08_02945"
     CDS             6726..8564
                     /locus_tag="PLU08_02945"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOQ70687.1"
                     /translation="MKIVSGNAPFLRSKRTTSQIMMELLIGLAIIFVAAVIYNFTLGV
                     RYGLKTICIMTVAILFTFISDIIAGSLRYNKEKDGKFDEYLIHFIKSNYSVVTAVIFA
                     LTLPIGTPVYVVIIGSMFATLVVKHAFGGFGSNVFNPAALSRIFLALTFGSQLKAYLP
                     GGENIGGLVAGATVTSQYAADGVKFLTGSLANTNVSMLDLYLGNHSGAIGETFTLLIL
                     IVGIVLAAREVINWRTPVFFLGTVALSSVFVSLVAKVNMIEYLLVQFGVGGMVFGAVF
                     MFTDPVTSPTSNYGKALIGIIGALINVLIRIAGVYPEGTAFSIAFVNIISPMIDRLIT
                     GRTNNKVWVPATVSGVMVLGSVGLITGVSAAKMPRVAPEPPPIVVEPFVTYLGSYTSP
                     TPSEYAEEFTTNVEVGLSDAYHIVKIEYDVIANPDNYKKVTDGIDAVIDYYTSISVAE
                     FKALPVPALSEGTTGSTVAGQTGEHIIPGAIYSSRALYEAINDALKDLNVYSADVEDY
                     PYDSGGYSDETLVMSVDVYVDTVTDLIKVVDIKDGASTGTYADRWETVKEAVLSKYHD
                     LDVATFLGYTNVAELYGEEVATYAGVTYSARRLFNAVQKALEGYGG"
     gene            8566..9123
                     /locus_tag="PLU08_02950"
     CDS             8566..9123
                     /locus_tag="PLU08_02950"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOQ70688.1"
                     /translation="MSKKKLNNFILLPLFLGAVTLVAAGALTGVHLLTKPKIEENIKQ
                     KQLSGYKAVLDLAQSDVIDLKKETVSDELAGIGVTNKFSVTIDTTLVGVVYDAEITGY
                     NSGLIFQVGFKGTKYAGFNVISSNETPGYGADYLETVHEQIKGVEIGDPILQDPSKTG
                     KTITANALRKALEACATDYLEGRAG"
     gene            9126..9815
                     /gene="rsxE"
                     /locus_tag="PLU08_02955"
     CDS             9126..9815
                     /gene="rsxE"
                     /locus_tag="PLU08_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HOQ70689.1"
                     /translation="MKKRKIFIDGIFANNAAFMLFLGMCPALATTSSFMSALGMGIAV
                     LATLILTNVVISAIRRWVPNEIRIPVLIVIIATTVTIIEMLMQAYMFDLSQTLGVYLS
                     LIVVNCIILGRAEAFAMKNNVLDSLIDAVGTGIGFMLGLLALAFAREFLGTGGIAFTS
                     LLTGQPIWGFQIIPDNYTIGLFQESSGAFIMFGLIVALINFVVAKISENKKKKEAAAK
                     AAAKLAAEGGK"
     gene            9818..10408
                     /locus_tag="PLU08_02960"
     CDS             9818..10408
                     /locus_tag="PLU08_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOQ70690.1"
                     /translation="MQFLLMGIGAVLINNIILTRFLGICPLLGVSKSNSSAIGMSLAV
                     IFVVTMSAILSYSLYMYVLVPLKIEYLRLVSFILIIASFVQFVEMFLKRFVPALYKSL
                     GIYLPLITTNCVVLAVSLDVVATNGGVPVYNFLNMVGYAFFTGVGYAVILILFSFIQE
                     RLINNKNMPKSFQGSGIALVTIGLLVMAFLGFAGMI"
     gene            10410..10592
                     /locus_tag="PLU08_02965"
     CDS             10410..10592
                     /locus_tag="PLU08_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70691.1"
                     /translation="MSDVARIFVAIGVMAILIALYIGSYVLNKRTKKPEGCKDFASSC
                     SGCAVTSCSHNPLKEE"
     gene            10596..10910
                     /locus_tag="PLU08_02970"
     CDS             10596..10910
                     /locus_tag="PLU08_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOQ70692.1"
                     /translation="MQYFWGVVILLVIGALLGLLLAVASKAFAVKEDPRLEPITEMMP
                     GINCGTCGYPGCKELVVAMLKGEVKNLGQCRPLRPDAKAKIKEYLANHPDEEGNILTV
                     QG"
     gene            10934..11347
                     /locus_tag="PLU08_02975"
     CDS             10934..11347
                     /locus_tag="PLU08_02975"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOQ70693.1"
                     /translation="MKARKVKLNKTLFDTIIFSIVLVLTSATTLYLKRAFSTTEALYV
                     YVYVTDRLIDKQPLAIDNTFIYNEPEFLAPITLEIYNKKVRVEKEESPLNLCSIKGFT
                     DKVGDPVLCLPNSFYFVIMGFNYKEAPSPYVPSVE"
     gene            11328..11882
                     /locus_tag="PLU08_02980"
     CDS             11328..11882
                     /locus_tag="PLU08_02980"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOQ70694.1"
                     /translation="MSQVSNNGNKRKLSTQKLVRLAVFLALGVVFNIVENYITFIPAL
                     PGVKLGLANSIGLILLALYGPLEFFTIGLLRVLLSGTFSGFGTSFILSLSGFLLSSAF
                     VLLAYLFNKLSIYGLSLLSAVMHGVGQVVMIAIIYQNALMLNYLIIMTISGIISGLLI
                     ALLSKIVILRLKMFKEEECNYEIN"
     gene            11869..13179
                     /locus_tag="PLU08_02985"
     CDS             11869..13179
                     /locus_tag="PLU08_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOQ70695.1"
                     /translation="MKLIKKLFALFSPLELVILMVSITISTLLVVPAFSKDTQKKTPI
                     LLFPTEKVYNTDGGRPIEVFDTSFFVVYEEVEEQTQNDIKAILNEYLISYHKLFDRHN
                     DYYAVEPHDNIHPTAEEKATLPRIKNLKYINEHKGEEIEIHQALYDLLVASKSYSINT
                     PNNAFSMFIGELYDYWKPYTSPGADPTKDPVRDDLKQAEIARLTSYIPQSEDDINNTL
                     KLWTNDNKYYVKFNEFNHSGDDLSISVGAVAKGMMTDILAEAMRAKGLTKGLIYGGQS
                     SFTFLEDGFMNKAYKITMEPIGPGEKTFTMSRADQYQMSTSGIYTGFKFNYGGAQIIR
                     SHIINPLTGYPAHNSHHMVNITSDTLSGLTLDYLTTALTVLSVQDGLTFLSEEYPSDD
                     INVIYSGYFNDEWFVAFTNGYPGGKTPSFQLAKEYREINLEFLL"
     gene            13252..14268
                     /locus_tag="PLU08_02990"
     CDS             13252..14268
                     /locus_tag="PLU08_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70696.1"
                     /translation="MKKRNLLLALVGSALLLTGCKGGSSEKPVSETPAVQKSFGFGSL
                     AKYAATRADNEFDIETTVDYVAVVLEADVIKNLRLDTVQLYAKIAEEAVVLKTETNGD
                     VKSKWELLDGYGMKVASPIEKEWYEQAEAFENWAVGKTVAEVKAGMTGTKLTDGATIG
                     NTIHVNAWVAALEDAVLNKVTFEGEVKGLGVGGVNAVSKDGATGAVEGQDWTIGGAVF
                     TEGKKVLAARIDTFQVRYENSEGKAVIKSGLKQVDYDNKRVKGKQELKEEYGMKVASP
                     IEKEWYEQAASLVEYIKGKTVSEALGTAAKLANGAEVGCTMNIGGYRTALLEAEHTAF
                     NSRK"
     gene            14348..15436
                     /locus_tag="PLU08_02995"
     CDS             14348..15436
                     /locus_tag="PLU08_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70697.1"
                     /translation="MGKKKKKTFKKFSATLGVIITLLAIVMYIPRTARIGYKNDKYAN
                     ILKDANIAADTRVVDIAMLGAHDAFSSNINLLSAPDPAESGIVKNKAVNAVFKGGLVR
                     VLKAQQDGAKVLLNRGVRYFDVRISYVEGEWYTKHGLLDTNLFYYLKDIYDFLENRPQ
                     EFVILDVQHIYTGESNINEFLNYLIDLRFVGNPLGLNDFIHHTSAIPLNDLLYFDVLG
                     PTHGGIVLLLKDDGSADATQKTLFYERNDAIRSKWHNKRYVRDIVPLINEEAAYLKTL
                     ADSDFFRVNQAQLTPDYLKDPLGTLGRWSLINIAATSNAKLLNNKAFDEWLSVMPIFM
                     VDFANSRYARFNTRVNEKIIEYNRNLGA"
     gene            15441..15917
                     /locus_tag="PLU08_03000"
     CDS             15441..15917
                     /locus_tag="PLU08_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70698.1"
                     /translation="MLKKYKFNLEIPAIILFAVILIPNLVWAIIPPAFDPLRVESVTK
                     GVDIATTIIQVLMIASLTLLTNEEASNVKQSPLLVTTLVMVLLYIISWVMYYASLFNI
                     VILLGLVLCPCVAIILYAIDRKNIFGLVLASLFTIGHFIFAFANFLCIVIPGACVY"
BASE COUNT         5100 a         2624 c         3309 g         4902 t
ORIGIN      
        1 aacccattaa aataagtcac ccagcagttg aagaatatgt ttacccgatt aatcgttccg
       61 tgttaattga aggagtgctc tatatttttt caacttatca cgttagtact tacgacttag
      121 cgacaaagat tagcactgct ccagtctaca tcgcttaact aattttataa gcactattta
      181 ccacgaaaat cactaagtaa gtagtgcttt ttgttcattt ttaacgacaa aacacgccta
      241 aattcaaaaa aatgagaaaa atatttcaac tcattgactc atcatcactt ttcaatgata
      301 taatgttagc aaaggggaaa acaacatgaa aattaaaacc agattagatt tcatcgtaat
      361 tgatggaacc gagctcgttg ttaatgaagc tctcaaaaca ctggcaaaat taccagtaag
      421 cgacttagtc gaatttttaa agggccctaa attatattca cgtcgagatc atgcaaacat
      481 tttaaagaat gttatgcgtg atgaagtctg gaagtttaaa cttgtctcac gcgttgcgga
      541 gaaaggactt tcaaatgatt tctttagtcg acttgattat tttgaagata gccctgtcac
      601 cgttcttgaa ggattatttg aacggatggt caagattcaa cccgaactaa tggattatta
      661 tcttgaagac ttatggctct ttgccattca agttgtaagc aaggacaaag aacttgctaa
      721 gctttataag aaagcacaaa aaagtagcac aagcccagtt gttcttaagg aattattccc
      781 gacacttgaa gggcttttcg gtgatgaaga aggcgatatc gacggtgtat tagcgcgcac
      841 atttgtggaa aagagcttac acagtgccac gattaaagaa atccgtgaaa ttgccgaaaa
      901 aaatgatgtt ttactaccaa caaatatcac aaaacaggac atagtggacg gaattattgc
      961 tgttttacca aaagtagcga aagcaaaaga tgtcccgcat ttaacggaag atgttaagga
     1021 tatgactgtt gcacaactga aagaatttgt gaacaagcat aaacttgatg ttccaactga
     1081 acttaaaaaa caagatgttg gtgaattact cgttaaaaac tatgtaaaca aaaatgcggc
     1141 aaatgtcgtt agtgatttac aatatgaatt accagaacgt gtcgaagaag ctactgattt
     1201 tagtgatgaa aatccaaaag ttatcaggtt gcgcgaacgc gttaaggaac tcgagaaaga
     1261 attagaagaa agacctgtcg aaaatgaaac aaaggttgat gaaagcgaac ttttaaaaga
     1321 acaagaaaaa gttgaagcac tttctaaaga attaaagcaa acaaaggctg agctttcaga
     1381 aaaaccaaaa gaagtcgtcg ttgaagtgcc aggaccaaca gtaattcgga cagtaattag
     1441 caaaaaagaa ttacaaaagc aaaaagaaca ctacgaagca attattgctg aacaagaaaa
     1501 agaacggtcc atgcttatta gccgtactga atacgatgaa gttttagcgg aatatggtcg
     1561 tgttaaagca ttacttgatg aaaaagacat tgagaagctt gaagaaaaac cagttgaacc
     1621 aaagcctgca ccgcaacaag tgccgcaata tgaagaacca tattacggtt cacttcacgc
     1681 taaagttgat cgcttaacgg ataaagttca tgaattgcaa ctagaaatct tacgtaaaga
     1741 attagctaat caaaaagcta ctccggcacc gcaagaagaa caacccgaaa cagaagttaa
     1801 tatttatttt gatagtaacc ttgctaaacg gatgtcggac aataaaaacg cgattgatgt
     1861 tggcaaagca gttaaaggta aaaaacgtaa aaagaagagt gttggacgga caatattctg
     1921 gattatcttt attctcttgc tcttagcaat agtcgtgttc atgggtgcat ggacattaga
     1981 gcatttcaat attacccctc ttgcaggacc accatggctc gctgatcgtt tcaacgctgc
     2041 ggtattatgg ctccacaacg ctgtccaaag tgtctttgat ttatttaaag gactatttgg
     2101 taaagtccgg gtattaggac gatagaaagg agagatttga gttatgagta aaaaacgtag
     2161 aattaaagaa ttagaagctc aagtagcagc gctcgaagca aaggttgaag gcaaagaagg
     2221 ccaacaaacg ccaaagaaag ccgctaaaaa ctccgtaaat gtctattttg atagtaactt
     2281 tgttaatagc ctagtatcaa atcaaatgaa gtatggacgg cgttcacgtc gtgatgattt
     2341 aaatgttgat cccgctgata aaaaggccgt cagaagagca cgcgctcgtc gtttcttccg
     2401 tttcttatgg agattaattg tagtgcttgt tattgccgct ttagtcgcag cggtcgtcgc
     2461 agcgatatta atggttatcg tctgggcact tgttaacttt aatattatga ccccaacatc
     2521 aaatccattt gtcggcatgg tctgggagtg gattaagtgg ttctttgccc tcttcgggat
     2581 ggaagtacca atcttctaga ttgacaccat ttagataaaa gcaaatcaaa gggaccccaa
     2641 gtcccttttt tgctataata aaaatgagaa gaatcacaaa tattgttata agtaaattaa
     2701 acccataagt tcaattaaaa aatatcatta caaaaacact atagcggaaa ggagtgcatg
     2761 cgatgtcaaa attaggcgac cgtatatttc aaaaagtatt gcgtaccttt agcccgattt
     2821 taccatggcg tgaaccagaa attttaaagg cgtatgatga attagttgat gttttaatta
     2881 agcaaaataa aagccgaatt tttattgtaa tggatgaagg tttagtccaa cttaaattac
     2941 atcaaccctt acttgacgcc cttaaaaata aacaactaaa ttatcgtgta tttagtgata
     3001 taaccgctaa tccgactaca gcccaagttg aagaaggact acttgcttac attatttatg
     3061 gtggtgacgc tattattggt tttggtggcg gcagtccaat ggatgttgct aaagccattg
     3121 ccgcccgtgt agcgcgacca cggaagaatt taaaacaatt aggcgggctc ttaaaagtac
     3181 ggaagaaaac accgctttta attgctgttc ccacaacttc aggaacagga agtgaagcga
     3241 cagtagcgtc gattgttgtt gacgagagaa caaagcacaa atatgcgatt aatgacccag
     3301 ttttaattcc aagttatgca ttattagtgc ctacactcac atttaaatta ccaaaatttg
     3361 tgacagcgac gacagggatg gatgccttaa cacacgctat tgaagcctac acaaataagg
     3421 gtggtcgtaa atttccaaat gaaaaagcga ttaacgctac acgattagtg tttgaaaatc
     3481 tcaaagtcgc atatgataat cctagtgacc ttgaggcacg aaaaaacatg caactagcag
     3541 cttatgatgc tggggttgct tttacgcgta attatgttgg ttatgttcat gccttaagtc
     3601 atccgcttag tgcatattac gggttagcgc atggctatgt taacgcttta atattaccgt
     3661 ttatgctaga gaaatatgga cgaagaattt accgaaaatt agcgcgactc gcgcggctga
     3721 gtggggttgt tgaagaagga ttaagtaacg gtgaaacagc taaacttttt atagcgaaaa
     3781 tccgtgcttt aaatacttac tttgaaatac cgacaacgat tccagaaatt aaagaagaag
     3841 acattccgca actagcagtg tatgctaaca aagaagcgcg ccccctctat cccactccgc
     3901 gtatttataa gacgagtgag ttagaagaat tttatcgaga attaaaagga gaaatattta
     3961 atgttgaaac aaataaaaac gcaaagagaa gcaatgaaaa aaaactttcc cttaagttat
     4021 gaaaaacggc gagaactatt aataaacctg caaaaagcag ttaaattata tgaaaaagac
     4081 atatatgaag ccctctacaa agatctccat aaagatgaat gggaagtatt tgtcactgaa
     4141 cttggtattg tgtatgaaga aatgcatttt gccataaaaa atctaaaaaa atggatgaga
     4201 agaaaaaaga ttaagtcatt tcttgtgcaa tttcccgcta aaaattttct tgtacctgag
     4261 ccctacggac aagtcttaat tatgagtcca tggaactacc cattacaact aacactttta
     4321 ccactgattg gagcgattgc ggcaggcaat tatgcggttg taaaaccaag tgcttacgca
     4381 ccagaaattg cgaaagtaat gcagcaaatt gttgcgactg cttttccaaa taaagaagct
     4441 attttatttc ttggtggtcg taaagtaaat gaagaaattt tacaaggtgt gtatgatttc
     4501 attttcttta ctggtagtcc tggagtcgga caaacggtga tgcgtaaagc aagtgaaaat
     4561 ttaacaccag tcgtccttga attaggcgga aagtcaccgt taataattgc agaagacgct
     4621 gatatcccct taacgattcg gcgccttaaa tttgctaaac ttattaattg cggacaaaca
     4681 tgtgtagcgc ctgactatgt gttacttgat aaaaaactta ttgataaagt tgatgatatt
     4741 cgtcaagcat ttagtgttga aggtgaagaa aaagcaaggt tacctaaaat tattaatgaa
     4801 aagcattact tgcgacttaa aggtgtttta actgaagtag gtagtgataa ttttgatgaa
     4861 gaaacacaaa ctattaaccc aacagttgtg ttaaatccac cacttgatag cgaactaatg
     4921 cgtgaagaaa tatttggtcc aattttaccg attattactt atgaaacact tgatgaagcg
     4981 attgctttta ttaatgaacg tcctaaaccg ctagcgcttt atcttttcac taaatcgaaa
     5041 gaaataatga ataaagttct aagagagaca acaagtggtt ctgtcgcgat taatgatgcg
     5101 gttgtgcaac tagcaaatcc gcacttcccc tttggtggtg ttggcaatag cgggatgggg
     5161 agttatcacg ggtatcatac ttttaaaaca tttagtcata tgaaggctgt tttacataag
     5221 tcccgtgctt ttgattttcg ttttagatat aaacctggtc caaaaacagt tggaattttt
     5281 aagaaattaa ctagaaataa ataaacatac attcattatt tatggtcgtt tcacttaaaa
     5341 gtgaactatt tacacattta ctttactttt tgctctttac gcgtgtataa tatgcgcgat
     5401 ggagggaaaa gtgatgaagt tattccaaac tgtaggtcgg cggaaaattc ctggctataa
     5461 aagtatgagt cttgaacgcg aacttgttgt tctcgaccct aaaaaagttg aatatgttta
     5521 ttttccgctt gtagccgcta ataatgtgcc gcttgaagtt ttcgtcaaag aaggcgataa
     5581 agtaaaagta ggaacaaaaa ttgcacttaa acagcaattt agtgttccgc tttttagtag
     5641 tgtctcggga actgttcgag caatcgaaaa acgcttgaat gttactacgg gacgtccttg
     5701 tgatcattta gtcattaaaa atgatttcaa aatgactaaa gaaaaaccat taaagaaagt
     5761 tgaattagat gcatcaaaag aagatattgt tgcagcaatt aaagaagctg tattgtcggt
     5821 cttggtggcg ctaattttcc aacatgggtt aaatacaaca atgtcaaaga tattcacact
     5881 atcttaatta atgctgttga atgtgaaccg tatttaacaa cggactacgt gtcttgtagt
     5941 cttaatgccg ctaaagttat tagtggtgct cgttatttac aaaaagccgc gggagcaagt
     6001 aaagttattg tggcaattaa agtaaaaaaa gaacaagctt gcaattgctt aaaggaagcg
     6061 gcagcccctt accctgacat tgaagtgcgt gaagtaccag atcgttatcc aatgggctgg
     6121 gagcgcacac ttattcaaac gatatttaaa aaacaatatg ataaattacc aagtgaaatc
     6181 ggtgttatcg ttaataacag cactaccgct attgcggtcc atgatgcgct tgttgaaggt
     6241 tcaccgattg tgtcacggat tgtgacagta agtggtaacg ccgtgaaaaa tgccgcaaat
     6301 attgtcgtgc ctgtcggtat tttagccgaa acagtgctta atgaatttga ggttgaagaa
     6361 aaagaagtgg tgctattagt cggtggtcca atgacaagta atcctgcaaa agatgtaagt
     6421 tttgccctta ctgtgtgtca tggtgccttg acactactag aaaaagtcgt ttatcgccca
     6481 gtagcatgtt tgcgctgtgg cagttgtaca gcacactgtc cagccggact ccaaccaatc
     6541 gaaatcttgc gcgcctttga aggtcgtgat tatgagcgac ttgaaaaact tgaaacatta
     6601 cgctgtgttg attgcggact ttgtagttat gtgtgtccga gtaaaatcga agttaccgat
     6661 atgatgaaaa aagctaagac cttacttcgc gttcatcaat cgaggaagaa atagaggtgt
     6721 ctaatatgaa aattgttagt ggaaatgccc cgttcttacg cagtaaacgc actactagcc
     6781 aaataatgat ggaactctta atcgggttag cgattatttt tgtcgccgcc gtcatttata
     6841 actttacgct tggagtacgt tacggtctta aaacaatttg tattatgact gttgcaatcc
     6901 ttttcacttt tattagtgat attattgctg gtagtttacg ttataacaaa gaaaaagacg
     6961 ggaagtttga tgaatattta attcatttca tcaaatcaaa ttatagtgtt gttacagcag
     7021 ttatctttgc tttaactcta ccaattggca ccccagtata tgtcgtgatt attgggagta
     7081 tgtttgccac tttagtcgta aaacatgcct ttggtgggtt tggtagtaat gtctttaacc
     7141 ctgctgcttt gtcgcgcatc tttttggcat tgacttttgg tagccaactt aaagcttatt
     7201 tacctggtgg tgaaaatatt ggtggcttag tagcgggtgc gactgtgact agtcaatacg
     7261 ctgctgatgg cgttaaattc ttaactggta gtttagcaaa cactaatgtg tcaatgcttg
     7321 acttatattt aggtaatcat agcggcgcga ttggtgaaac atttacctta cttattttaa
     7381 ttgtcggtat tgtcttagcc gcccgtgaag taattaactg gcgcactcca gtcttcttct
     7441 taggcacagt tgcacttagc agtgtctttg ttagcctcgt agcgaaagtg aatatgattg
     7501 aatatttatt agtgcaattt ggtgttggcg ggatggtatt tggtgctgtc ttcatgttta
     7561 ctgatccagt tacttcacca acgagcaact acggtaaagc gctgattgga attattgggg
     7621 cattaattaa tgtcttaatt cggattgctg gtgtttatcc agaaggtaca gcttttagta
     7681 ttgcatttgt taacatcatt tcaccaatga ttgaccgttt aattacgggc cggacaaata
     7741 ataaagtatg ggttcctgct actgttagtg gggtgatggt attaggtagt gttggcttaa
     7801 ttaccggcgt tagtgcggca aagatgccac gcgtcgcgcc agaaccaccg ccgatcgttg
     7861 ttgaaccgtt tgtgacttat ttaggaagtt atacaagtcc gacaccaagt gaatacgctg
     7921 aggaatttac gacaaatgtc gaagttggat taagtgatgc ttatcatatt gtaaaaattg
     7981 aatatgatgt gattgcgaat ccggataatt acaaaaaagt aactgatggg attgatgcgg
     8041 tcattgatta ctatacctca attagtgtcg ccgaatttaa agccttacca gtcccagctt
     8101 taagcgaagg tacaaccggc tccactgttg ctggtcaaac aggcgagcat attatcccag
     8161 gagcgattta ttcttctcgc gcactttacg aagcaattaa tgatgcactt aaagacttaa
     8221 atgtgtatag tgctgatgtt gaagactacc cttatgatag cggtggttat agtgatgaaa
     8281 cattagtgat gagtgttgat gtttatgttg acactgtgac tgatttaata aaagttgtgg
     8341 atattaaaga tggcgctagt actggtactt acgctgatcg ttgggaaacg gtaaaagaag
     8401 cagtactaag caagtatcat gatttagatg ttgccacatt tttaggttac acaaatgttg
     8461 ctgaacttta tggtgaagaa gtagcaactt acgctggtgt aacttattcg gcaagacggc
     8521 tctttaatgc cgtgcaaaaa gcactagaag gatatggtgg ctaatatgag taagaaaaaa
     8581 ttaaataact ttattttatt gccgctcttt ttaggagcag taactttagt agcagccgga
     8641 gcattaaccg gagtgcattt attaactaaa ccgaaaattg aagaaaatat taagcaaaaa
     8701 caactttcgg gttataaggc tgtgcttgac ttagcgcaaa gtgatgttat tgatttaaaa
     8761 aaagaaacag tcagtgacga attagcgggt attggtgtca ctaataaatt tagtgtgaca
     8821 attgatacca cgctagttgg tgttgtttat gatgctgaaa ttactggtta taattcaggc
     8881 ttaattttcc aagtcggctt taaaggtaca aaatacgctg ggttcaatgt tatttcatca
     8941 aatgaaacac ctggatatgg cgctgattat ttagagacag ttcatgagca aattaaaggt
     9001 gtggaaattg gtgatccaat tttacaagat ccaagtaaga caggtaaaac aataaccgcg
     9061 aatgcactgc gtaaagcact tgaagcctgt gctacagatt acttagaagg gagagcgggt
     9121 taattatgaa aaaacgtaaa atctttatcg atggtatttt cgctaataat gctgcattta
     9181 tgctatttct aggtatgtgt cctgccttag cgactacaag ttcatttatg agcgcgctcg
     9241 ggatggggat tgccgtatta gcaacactta tcttaacaaa tgtagtgatt tctgcgatta
     9301 gacgctgggt ccctaatgaa attagaattc ccgtattaat tgttatcatt gctacaaccg
     9361 taacgattat tgaaatgctg atgcaagctt atatgtttga tctttcacaa acacttggtg
     9421 tgtatttatc actgattgtt gttaactgta ttatcttagg tagagccgaa gcctttgcaa
     9481 tgaaaaataa cgtattagat agtcttattg acgcggttgg aacgggcatt ggctttatgt
     9541 taggattgct agcgttagcg tttgcccgtg aatttttagg aacaggcggg attgccttta
     9601 cttcactttt aacagggcaa ccaatttggg gtttccaaat tattcctgat aactacacaa
     9661 ttggtttatt ccaagaatct tctggtgcct ttattatgtt tgggctcatt gttgccttaa
     9721 ttaattttgt ggtggcaaag attagtgaaa ataagaagaa gaaggaagcc gcagcgaaag
     9781 ctgccgctaa acttgccgca gaagggggta aataattatg caatttcttt taatgggtat
     9841 tggcgctgtt ttaattaata atattatttt aacgcgcttc ttaggtattt gtccgctact
     9901 tggtgtttcg aaaagtaata gtagtgctat tggcatgagt ttagctgtta tctttgttgt
     9961 tactatgagc gcaattctta gttactcact ttatatgtat gtgttagtgc cacttaagat
    10021 tgagtactta cgactcgtgt cctttatttt aatcattgct tcattcgtac aatttgtaga
    10081 aatgttctta aaacgttttg tgccagcttt atataagagt cttggcattt atttgccact
    10141 tattacgaca aactgtgtcg tgttagcggt tagtcttgat gttgtagcca caaatggcgg
    10201 agtcccagtt tataatttct taaatatggt tggctatgct ttctttactg gtgttggcta
    10261 cgctgtcatt ttaattctct ttagttttat tcaagaacgc ttaattaata ataaaaatat
    10321 gccgaagagt ttccaaggta gcgggattgc tttagtaaca attggtttac tagtgatggc
    10381 cttccttgga tttgcaggga tgatttaaga tgagtgatgt tgcgcgtatt tttgtggcaa
    10441 tcggtgtgat ggcgatctta attgcccttt atattggaag ttatgtgtta aataaacgca
    10501 caaaaaaacc agagggttgt aaagatttcg ctagtagttg tagcggatgt gcggtgacat
    10561 catgtagtca caatccacta aaggaggaat aatttatgca atatttttgg ggagtagtta
    10621 tccttttagt tattggagcc ttattaggac ttttacttgc cgtcgcgagt aaagcttttg
    10681 cggtcaagga agacccacgc cttgagccaa ttacggagat gatgcctggt attaattgcg
    10741 ggacttgtgg ttatccggga tgtaaagaac tagtagtggc gatgcttaag ggtgaagtga
    10801 aaaatttagg tcaatgtcgg ccgttgcgtc ctgatgcaaa agcaaagatt aaagaatatt
    10861 tagcaaatca tccggatgaa gaagggaaca tccttaccgt tcaaggataa aaagttaggt
    10921 tactcttatt tacatgaagg cgcgtaaagt taagttaaac aaaacacttt ttgacaccat
    10981 tatctttagt attgtcttgg tgctaacaag tgcgacaacg ttgtacttaa aacgcgcctt
    11041 ttctactact gaagcactgt atgtttatgt ctatgttacc gaccgtctaa ttgataaaca
    11101 accgttagcg attgataata cattcattta caatgaacct gaatttttag caccaattac
    11161 acttgaaatt tacaataaga aagtgcgggt tgaaaaagaa gaaagtccgc ttaatttatg
    11221 ctcaattaaa ggttttacgg ataaagtagg tgatcccgta ctttgtctac ctaattcttt
    11281 ttattttgtg attatgggat ttaattataa ggaggcacct tcaccgtatg tcccaagtgt
    11341 cgaataacgg aaataaaaga aaattatcga cacagaagct cgtgcggctt gctgtctttt
    11401 tagcgctagg tgtggtattt aatattgttg aaaattacat tacatttatc ccggctttac
    11461 cgggtgttaa attaggcctt gctaattcga ttggcttaat tcttttagca ctttatggtc
    11521 cgcttgaatt tttcacgatt ggcttacttc gagttttatt aagcgggact tttagtgggt
    11581 ttggcactag ttttatttta tcgcttagtg gttttttact ttcaagcgct tttgtactat
    11641 tagcctatct ttttaataaa ttatcaatct acggactctc actccttagt gcggtgatgc
    11701 acggtgttgg ccaggtcgtc atgattgcga taatttatca aaacgcttta atgcttaatt
    11761 atttaataat tatgacaata agtgggatta taagcggatt acttattgct ctcttatcaa
    11821 aaattgtgat tttgagactt aaaatgttta aggaggaaga gtgtaattat gaaattaatt
    11881 aaaaaactct ttgcgctttt ttccccgctt gaactagtta ttttaatggt ttcaattaca
    11941 atctcaaccc ttttagttgt tccagcgttt agcaaagata cgcaaaaaaa gacaccgatt
    12001 ttattatttc cgacagagaa agtttataac actgatggtg ggcgtcccat tgaagtattc
    12061 gacactagtt ttttcgtcgt gtatgaagaa gtggaagaac aaacacaaaa tgacattaaa
    12121 gctattctaa atgaatactt aattagttac cataaacttt ttgaccgtca taatgattat
    12181 tatgcagttg aaccccacga caatattcat ccgacagcgg aagaaaaagc gacactgcca
    12241 cgcattaaaa acttaaagta tattaatgaa cataaaggcg aagaaattga aattcatcaa
    12301 gctctttatg acttacttgt tgcgtcaaaa agttattcta ttaacacacc aaataatgct
    12361 tttagtatgt ttattggtga actttacgat tactggaaac cttacactag tccgggggct
    12421 gacccgacta aagatccagt gcgtgacgat ttaaaacaag cggaaattgc acggttaacg
    12481 agttatatcc cgcaaagtga agacgatatt aataacactt taaagttatg gactaacgat
    12541 aataaatact atgttaaatt taatgaattt aatcatagcg gtgacgatct ttcgatttct
    12601 gttggtgcgg tagctaaagg gatgatgact gatattctag ccgaagctat gcgtgctaaa
    12661 ggactaacta aaggtttgat ttacggcgga caatcgtcat ttacattttt agaagacgga
    12721 tttatgaata aagcttataa gattacgatg gaacctattg gaccaggcga aaaaacattt
    12781 acaatgagcc gcgctgatca ataccaaatg tcgacaagtg ggatatatac gggctttaaa
    12841 tttaattatg gtggtgcgca aattattcgt tcgcatatta ttaatccgct aactggttat
    12901 cctgcgcata actctcatca tatggttaac attacaagtg acacgctaag cgggttaacg
    12961 cttgattacc taacaacagc attaaccgta cttagtgttc aagatggatt aacattttta
    13021 agtgaagagt atcctagtga tgacataaat gtgatttata gtggttactt taatgacgaa
    13081 tggtttgtgg cctttacaaa cggttatcca ggcgggaaaa caccatcatt tcagttagcg
    13141 aaagaatacc gcgaaataaa tctcgaattc ttactataaa ggttgctttt cttaataata
    13201 tttattaaaa ttatatgcgc agtaagaaga aaatagagga gaatgatata tatgaaaaaa
    13261 agaaacttat tactagcatt agtcggtagt gcattgctat taactggctg taaaggcgga
    13321 agtagtgaaa agccagtttc agaaacacca gcggtgcaaa aatcatttgg ttttggcagt
    13381 cttgcaaaat atgccgcaac acgcgctgat aatgaatttg atattgaaac aactgttgat
    13441 tatgtcgctg ttgtcttaga agcagatgtt attaaaaact tacggcttga cactgtccaa
    13501 ctttatgcaa aaattgctga agaagcagtt gtacttaaaa ccgaaactaa tggtgatgtt
    13561 aaatctaaat gggaactttt agatggttac ggcatgaaag ttgcgagccc aatcgaaaaa
    13621 gaatggtacg aacaagctga agcatttgaa aattgggccg ttggtaaaac agttgccgaa
    13681 gttaaagctg gcatgactgg tacgaaatta actgatggtg caacaattgg taatacaatc
    13741 catgttaacg cttgggttgc cgcccttgaa gacgctgtcc ttaataaagt tacttttgaa
    13801 ggtgaagtta aaggattagg tgttggtggt gttaacgctg ttagcaaaga cggcgcaact
    13861 ggtgctgttg aaggacaaga ctggaccatt ggtggtgctg tctttaccga aggcaaaaaa
    13921 gtcttagcgg cacgcattga tactttccaa gttcgctatg aaaatagtga aggtaaagca
    13981 gttattaaat ctggcttaaa acaagttgat tatgataata aacgcgtaaa aggtaaacaa
    14041 gaacttaaag aagaatatgg catgaaagtt gcaagcccaa tcgaaaaaga atggtacgaa
    14101 caagctgcaa gcttagtcga atacattaaa ggtaaaaccg ttagcgaagc tttaggaact
    14161 gctgctaaat tagcaaatgg tgctgaagtt ggttgtacta tgaatattgg tggctatcgc
    14221 actgctttac ttgaagctga acacacagcc tttaatagtc gtaaataatt aattagttac
    14281 gaagaaaaga tacacaaggg actacttcaa agtagttcct tttttgttaa tatgcgataa
    14341 tattactatg ggaaagaaaa agaaaaaaac atttaaaaaa tttagtgcga cgcttggagt
    14401 tatcattaca ttactcgcaa tagtaatgta tataccgcgt actgcgcgta tcggttataa
    14461 gaatgacaaa tatgcaaata ttttaaaaga cgctaatatt gccgctgata cgcgtgtagt
    14521 ggatattgcc atgctcggag cgcatgatgc ttttagcagc aatattaatc tacttagtgc
    14581 ccctgatcca gccgaatcag gaatagtgaa aaataaggcc gtaaacgccg tttttaaagg
    14641 cgggttagtg cgagtgttaa aagcacagca agacggcgct aaggtgctac ttaatcgtgg
    14701 tgtccgctat tttgatgtgc ggattagtta tgttgaaggt gaatggtata caaagcacgg
    14761 tttacttgat acgaatctct tttattattt aaaagatatt tatgactttt tagaaaatcg
    14821 tccacaagaa tttgtgattt tagatgttca acatatttat acaggcgaaa gtaatattaa
    14881 tgaattcctt aattatttaa ttgaccttcg ttttgttggt aatccgttag ggctaaacga
    14941 ttttatccat cacacaagcg caataccgct taatgattta ctttactttg atgttttagg
    15001 accgactcac ggcggtatag tattattact gaaagatgac gggagcgctg atgcaacgca
    15061 gaaaacacta ttttacgaac gaaatgatgc gattcgttca aagtggcata acaagcgtta
    15121 tgtaagagat attgtgccac ttattaatga agaagcagct tatcttaaaa ctttagcgga
    15181 tagtgatttc tttagagtca atcaagcaca actcacacct gattatttaa aagatccact
    15241 agggacatta ggtaggtggt cacttattaa tattgccgcg actagtaacg ctaaattatt
    15301 aaataataaa gcttttgatg agtggctaag tgtgatgcca atattcatgg ttgatttcgc
    15361 taatagtcgc tacgctagat tcaatacacg cgttaacgaa aagataattg aatataatcg
    15421 taatttagga gcgtaaagtt atgcttaaaa aatataaatt taatttagaa ataccagcaa
    15481 ttatcctttt tgctgttatt ttaattccta atttagtgtg ggctataata cctcccgctt
    15541 ttgacccttt aagagttgaa tcagtgacaa agggtgttga tatagcgaca actataatac
    15601 aagtactaat gatcgcatct ttaactctgt taacaaatga agaagctagt aatgtaaaac
    15661 aaagcccgct tttagtgaca acacttgtaa tggtcttact atacataatt agttgggtaa
    15721 tgtattacgc tagtttattt aatattgtaa ttcttttagg actagtgtta tgtccgtgtg
    15781 ttgctattat cctttatgct attgaccgta aaaacatatt tggtttagtg cttgcttctc
    15841 tttttactat cggtcacttt atttttgcgt tcgctaattt tttgtgcatt gttatccctg
    15901 gagcgtgtgt ttattaactt aaaagttaca attta
//
LOCUS       DAOSTA010000020        15598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_3888_length_15598_cov_9.915396, whole genome shotgun sequence.
ACCESSION   DAOSTA010000020 DAOSTA010000000
VERSION     DAOSTA010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 15598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15598
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3888_length_15598_cov_9.915396"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1045
                     /locus_tag="PLU08_03005"
     CDS             122..1045
                     /locus_tag="PLU08_03005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ70699.1"
                     /translation="MKKKISLIIPCYNEEEMIDILYDEINKVLVKLPKYDFIFLFVDD
                     GSKDQTLSLIKAKAKKDHRVKYISFSRNFGKESSMLAGLEAAQKLDVDAALLMDADMQ
                     DPPSLMLEFFKYYEEGYKYIFTRNKTRKGQALLKRFFANAFYVIYAWLTGDKNSVHSA
                     RDFALLDRDVINAFLTYKDKHRYSKGISSQIGFKKKCIEYDFQERQAGTTKWSFRKLF
                     KYAMTGIKQFSHVYILVPNLISIFLSIALILQIIFGHIYKYDYRYIYIAITAGVLLLN
                     ITLKYVMKLGYDIRDQLLNRPHYLTAETNIE"
     gene            1148..1816
                     /locus_tag="PLU08_03010"
     CDS             1148..1816
                     /locus_tag="PLU08_03010"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOQ70700.1"
                     /translation="MSKEKKQISKNLVYRVPVYLTVSRRLLKDGARYITAPQISAITG
                     INVETVKKDLSQICKTPGNPKLGREADVLVSEIIEFGVYTKLTNAVIIGAGHLGSALL
                     NYDGFNEWGLQIIAAIDNNPALINTTINDKQIHGVDDLDKIIKEYNVQIAIITVPKEY
                     AQELVDHAVAAGVKAIWNFAPTHISVPKDVVLQNENMASSLSLLNYNLNLKTSKQKRK
                     TKRK"
     gene            complement(1864..3567)
                     /locus_tag="PLU08_03015"
     CDS             complement(1864..3567)
                     /locus_tag="PLU08_03015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOQ70701.1"
                     /translation="MKFSQYPFNVPDVPKIQKQLTKYIEQFKNAKDTNAASRVMRKIS
                     KYVDDFVTDAVIISVKFSQDSRNEEYVKAQEYVDHHFPYLSALMNEYNKLLVASPFRA
                     ELEKKWGSYLFKMIETQLKTFDPSIIELLQKESQLTNEYSKLIASAQIKFDGEVYNIP
                     QMGKFTQDKNRDVRKRASAAVVKFFEENNDELGRIYHELVQVRHAMAVKLGYKNFIEL
                     GYLKLGRLDYTAKEVAGYRDQIYREVVPLAHKLYRTSLRRAGIKSPRHYDLNQSFSDG
                     NPVPLGDSKYLVKAATKMYDELSPETGEFFRFMVDKELLDLDARPGKRGGGYMTYFPR
                     YQAPFIFANFNGTAGDVDTLTHEVGHAYQGYESRHIKVSEYRQPTLEACEIHSMSMEF
                     FAWPWMESMFDDADKYRLSHLEGTIKFLPYGVAVDEFQEGVYANPEMTHEERCAMWRE
                     IEKKYLPIRNYKGAPFLEKGTFWLRQSHIFSSPFYYIDYTLAQVVAFQFATEMWKNRD
                     RAWNKYVKLCRLGGRYPFVELLAKAKLRNPFEDGNVRRVITPLKRKLKELTQVLESKN
                     D"
     gene            complement(3582..5183)
                     /gene="gpmI"
                     /locus_tag="PLU08_03020"
     CDS             complement(3582..5183)
                     /gene="gpmI"
                     /locus_tag="PLU08_03020"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOQ70702.1"
                     /translation="MNKRPIILTILDGYGYSEETKGNAVKKAKTPNINAFYKNNPTVL
                     IEASGEAVGLPAGQMGNSEVGHQNIGAGRIVYQSLTYINQLIKDGRFFENEVFVNAVK
                     HTLKYNSKLHLMGLVSDGGVHSHINHIKAFLDLAKMHGAKEVYLHALMDGRDVAPQAG
                     PEYIQELVDYMAKINFGKLATIGGRYYGMDRDKNLNRTNVHYDAIVNRKGLFFSDFRE
                     YFKEQYETLPKNGFDPSDEFLVPGINKNVNGQLGDNDAVIFFNFRPDRAIQFGTIVTN
                     PYFYQNPPLKDGKPEYKAFVPSPILKNIYYACTMKYADSVHGHIALSLPKLDNGLGVF
                     LADKGYTQLRIAETEKYAHVTFFLDGTVNYDGVEKPELKGARRILVPSPKVATYDLQP
                     EMSAPGVTDALLKELDKGDLDVVILNYANPDMVGHTAIESAVIKALETVDACVGRILE
                     WVEKHDATIMITADHGNSEKLLDEEGKPFTAHTSNPVRFGINRKDIKLMKEGGKLSNI
                     APTIIELLGEVPPSEMTEPSLIIKN"
     gene            complement(5255..5728)
                     /locus_tag="PLU08_03025"
     CDS             complement(5255..5728)
                     /locus_tag="PLU08_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70703.1"
                     /translation="MQIVRIIILVLVVIYLLLAFVFMHISLDYTRQLKKSKETIHSLF
                     AGQIALFAMIGKELESPNSEAQVMNELLEKREFTELNKLAAEKERAYQELAAKKKDTT
                     PQTAQLLQGLSENVVLIRNEIYRHNKLVDNINVNVDSVIFSLFVVILRLKRLTKI"
     gene            5913..6581
                     /locus_tag="PLU08_03030"
     CDS             5913..6581
                     /locus_tag="PLU08_03030"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOQ70704.1"
                     /translation="MENIVRMPILGERFPELDTMTTFGKKKLPNDYEGKWFILFSHPG
                     DFTPVCTTEFIAFNEEIEEFRKMNAELIGLSIDQLQSHMKWTEFIGKITGKEIGFPII
                     ADELGRVANALGMVHPGKGTNTVRAVFIVDPKGVLRLMMYYPQEVGRAVYEVKRALNA
                     LQVADEHKVAAPENYPNNAFLGKDMVILPPPGTEKQKADRLALLERGEAKSYDWWFSY
                     KKLK"
     gene            6735..7568
                     /locus_tag="PLU08_03035"
     CDS             6735..7568
                     /locus_tag="PLU08_03035"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOQ70705.1"
                     /translation="MGTAKPIIAIIYDFDKTLTTTDMQNYNFIPALGMTPEEFWGETG
                     KFSDKTGVERILSYMYMMIVKAREKGINLTKEFLYEQGKNMEYFDGVLTWFDRINEYG
                     EKKSIKIEHYLISSGTKEIIEGSEIAKHFKAIFGCEFYFDPQTKLPVWPKLAINYTAK
                     TQFYFRITKGILDVSEDNALNRKLVDHRIPHRNIIYIGDGMTDVPVMLLVKQNGGHSI
                     AVYPKGEREKVEYLYTDGRVNFVARADYTEKSDINSIVRLIIDQVSIAEQIEKKKQLL
                     N"
     gene            7571..7942
                     /locus_tag="PLU08_03040"
     CDS             7571..7942
                     /locus_tag="PLU08_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOQ70706.1"
                     /translation="MNIYELNEIVEGTITEIRPYGAIITFPENRTGLLHIKQISDAYI
                     SNINTYLQLDAQIKVRIIEIDEHNGFLKLSLKSVPYNERIIFERDKKATNINPAQTDF
                     SALEKALPLWIEKAIKEAQND"
     gene            7935..9212
                     /locus_tag="PLU08_03045"
     CDS             7935..9212
                     /locus_tag="PLU08_03045"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOQ70707.1"
                     /translation="MIRLNYDNVLTTIDFEKYRSKLEKINEGINQKKGAGNDYLGWAD
                     WPLHYDKKEFNLIKETALMIRETFDTLVVTGIGGSYLGARAAIEAINGLLPQKGPEII
                     FLGHTFSPTLTAQTLEYLKTKNFAINVISKSGTTTETSIAFRLLKQLLIEKVGEANAN
                     KAIFATTDAKTGALRKLATQNGYTTFTLPSDIGGRYSVLTAVGLLPLAVAGIDIDDLM
                     AGALKARSESVDNLDHDLYKYAVIRDTLYRQNYKSELYVVYEPQYAMFNEWLKQLYGE
                     SEGKEKKGLFPTSVTFSTDLHSLGQFIQDGSPILFETTLFVENPNQDVVIPSEKENLD
                     ELNYLAGKNLALVNKQAFKGTLAAHVEDGKVPNLVFEIATLDAHTLGYMFYFFMRSCA
                     VSAYLLDINPFNQPGVEVYKKNMFALLGKPSRK"
     gene            9298..9373
                     /locus_tag="PLU08_03050"
     tRNA            9298..9373
                     /locus_tag="PLU08_03050"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9331..9333,aa:Val,seq:tac)
     gene            9394..9477
                     /locus_tag="PLU08_03055"
     tRNA            9394..9477
                     /locus_tag="PLU08_03055"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9428..9430,aa:Tyr,seq:gta)
     gene            9487..9562
                     /locus_tag="PLU08_03060"
     tRNA            9487..9562
                     /locus_tag="PLU08_03060"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9519..9521,aa:Gln,seq:ttg)
     gene            9566..9641
                     /locus_tag="PLU08_03065"
     tRNA            9566..9641
                     /locus_tag="PLU08_03065"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9599..9601,aa:Lys,seq:ttt)
     gene            9644..9731
                     /locus_tag="PLU08_03070"
     tRNA            9644..9731
                     /locus_tag="PLU08_03070"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9678..9680,aa:Leu,seq:taa)
     gene            9823..10062
                     /locus_tag="PLU08_03075"
     CDS             9823..10062
                     /locus_tag="PLU08_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOQ70708.1"
                     /translation="MEDLKQTENKIKVLLNRIRPYIQSHGGDFEFVRFEEGFVYLKML
                     GACEGCPLVNQTLEYGIEEILMEEVPGVLGVLLDE"
     gene            complement(10239..10889)
                     /locus_tag="PLU08_03080"
     CDS             complement(10239..10889)
                     /locus_tag="PLU08_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70709.1"
                     /translation="MKRKGSLLILIIPMLFSLTACNSGLNEYRENAKAEIARFVTDWQ
                     HIFNDHQEALNIAVQKGEEAINKARNKKEIDSIVKNTKADIDALLINDDYEFSISTEM
                     VVKKSGEDFSVEVKLKNNSGADIEMSYFWSPFWPFAPRWQYPLFIEPSEFPETVTFLN
                     NETYTETWTLGANFEVGKHDLRFKALFYPNYYEDDTNCVVIWSPHIEIAVDYYLGK"
     gene            complement(11028..12554)
                     /locus_tag="PLU08_03085"
     CDS             complement(11028..12554)
                     /locus_tag="PLU08_03085"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOQ70710.1"
                     /translation="MKKKTIILMIPLLIFTLVVSGCERGSESLSDNSRPTLGSEEPTS
                     TTTTSTTSTTITSTEGRTGQKELSFYATNDFHGSIVETNSEPGIFKLGSYLKSEFAAK
                     RNKTVYVDAGDIWQGSADSNINRGKFLVEAMNVLKLDAQTIGNHEFDWYDTVIETNKE
                     LANYPFLGANIMVRETGELATNLVSYDETFKASTMVTRNDVNIGIIGTIGMLEGSILA
                     GAIAPYTFKPVTTYIEREAENLRAQGADMIVLSTHDSLANSLGAYAPIIENKVVDIIF
                     SGHHHVRDNQFINDVPIMQTEGNGKQVMEVHGYYDFNLKEFKVTSREFTEAADIKRDY
                     TEDTELLNLFNEKYAEEIERVKGEVIGSLTNYINKSTLVKLANKVMYNFASITHNPDT
                     IIAVHNYGGVRVDGIDAGEVTYGDIYKAFPFDNEIKMFEDVLGSTASSFIGSNASYPI
                     GKFDIQNDQEYTIVTIDYVSDSKYSNIRDYPHTVTGEYVRELIAEYFRTNGEIDPYTI
                     "
     gene            complement(12551..13333)
                     /locus_tag="PLU08_03090"
     CDS             complement(12551..13333)
                     /locus_tag="PLU08_03090"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HOQ70711.1"
                     /translation="MKYKKELNAAIEAGKIARDIVLKIYESDFDVEIKDDNSPVTLAD
                     KMADKAMREYLGPLFPDYAFLTEESDDDLSRLTTDYVWIIDPVDGTKDFIHRDGEFTV
                     CIALTYKGEVVVGVIVVPVLNEIYYATKGGGAYELFADGSTKKLSVNDKTSNLTATRS
                     RHHFKPADNEYIEKHKDLISNVITYGSTLKAIKIAKGEIELFYRSGPGTKEWDIAAAD
                     IIVTEAGGIFIGPDGKKFTYNKADIHNRNGYLIVNRKENIRL"
     gene            complement(13323..14264)
                     /locus_tag="PLU08_03095"
     CDS             complement(13323..14264)
                     /locus_tag="PLU08_03095"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOQ70712.1"
                     /translation="MFKNLRKFIEEHDKIAIFSHIYPDGDAVGSIIGLRELIKTSYPH
                     KEVFGLGSNVQPFVSIVGELDEVSDDFIKESAAIIVDVANGERVEDQRFKLAKASFKI
                     DHHIFVEKFTDEELVLTKRIATAEIIGELMMKEKLRTNKLGATALALGIITDSGRFRY
                     DLTSGNTFSVMARLTNIGADLALINENLAKRSLADFKKRGHFLSNYETYENVIYIIVP
                     YLKLQELDILPSEGSNYVNTYSNLDDYPLWATFFIEKDGRVFVELRSKNYNVQKVATL
                     FGGGGHLKASGCRLENEARIADVLQQLTKAEELKDEV"
     gene            complement(14265..15122)
                     /gene="fba"
                     /locus_tag="PLU08_03100"
     CDS             complement(14265..15122)
                     /gene="fba"
                     /locus_tag="PLU08_03100"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOQ70713.1"
                     /translation="MPLVSMKEMLIKANKEKYAVGQFNINNLEWTQAILDEVQELQAP
                     VILGVSEGAAKYMGGYNVVVQMVKALIKDRGYTIPIAIHLDHGSSEEVCKKAIDAGFT
                     SVMIDASHHPFKENLEITKRVVDYAHARGVSVESELGSIGGQEEHVIAETLYADPNEC
                     LEMVQKTKIDCLAPALGSVHGPYKGEPKLGFDEMDHINKLVGVPLVLHGGSGIPDFQI
                     QMAIERGTAKINVNTEIQQEWTKIVRETLANNDKVYDPRKIIGPGMKGIRKVVNEKCR
                     VFGCIGKAA"
     gene            complement(15152..15538)
                     /gene="rpoE"
                     /locus_tag="PLU08_03105"
     CDS             complement(15152..15538)
                     /gene="rpoE"
                     /locus_tag="PLU08_03105"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR04567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HOQ70714.1"
                     /translation="MKKRTNIEIAYTLLKEHGESLSFYDLWQGVVNEHEYSEAEGHDL
                     ISRFYTALTMDGRFVNLGDNMWALRENVLFDDIALPLNEVYTEVDEAGDEEEEDEGPE
                     ILYFDDETGQADEAKVRSTLDEEDEE"
BASE COUNT         4904 a         2995 c         2630 g         5069 t
ORIGIN      
        1 aaaaaatgat tctttcagtt ttttatcttg tcaatttaac cttttcttct attatgaaaa
       61 taataaattt ttacttacta tccataaaat gatatcattt tattaattaa gggagttatt
      121 tatgaaaaag aaaataagcc tcataattcc ttgttataac gaggaagaaa tgattgatat
      181 tctctatgat gaaattaata aagttttagt aaaattacca aaatatgatt ttatctttct
      241 ttttgttgat gacggatcaa aagatcaaac actgtcatta atcaaagcaa aagcaaagaa
      301 agatcatcgc gttaaatata tttctttttc gcgaaacttt ggcaaagaat cttcaatgct
      361 tgccggcctt gaagccgcac aaaagcttga tgttgatgca gcgttactta tggacgctga
      421 tatgcaagat ccgccatctc ttatgcttga attttttaaa tattatgaag aaggctataa
      481 atatatcttt acccgtaata agacacgcaa aggacaagcg cttttaaaac gcttcttcgc
      541 taacgcattt tatgtgattt atgcttggct tacaggggac aaaaatagtg tgcatagtgc
      601 ccgcgatttt gcccttcttg accgtgatgt tataaatgct ttcctgactt ataaagataa
      661 acatcgttat tctaaaggaa tctcttcaca aattggtttt aaaaagaaat gcattgaata
      721 tgatttccaa gaacggcaag ctggcactac taaatggtcg tttagaaagc tatttaaata
      781 tgcgatgacg gggattaaac agttttcaca tgtttatatt ttagtgccta acctcatcag
      841 catcttctta agcattgctt taatcttaca aattatattc ggccatattt ataagtatga
      901 ctatcgttat atttatatag caattaccgc tggggtttta ttgctgaaca ttacgcttaa
      961 atatgtcatg aagttaggat acgatattcg tgaccaactc ttaaatcggc cacattactt
     1021 aactgctgaa acaaatatcg agtaaaaata ccgatatgct tatatcaatg ttgttatttt
     1081 taaatgtttt cctttataat aaggctgacg gaaaattttc ccgtaatgca gaaaggtttc
     1141 tttttttatg agtaaagaga aaaaacaaat atccaaaaac ctcgtatacc gtgtccctgt
     1201 atatttaacg gtctcgcgcc gtcttttaaa agatggtgcc cgctacataa ctgcaccgca
     1261 aattagtgcg attaccggga ttaatgtcga aacagttaaa aaagatttat cgcaaatttg
     1321 taaaactcca ggtaacccaa aacttggccg tgaagctgat gttttagtat ctgaaattat
     1381 tgaatttggt gtgtacacaa aattaactaa tgccgttatt attggtgctg gtcatcttgg
     1441 tagtgccttg cttaattatg atggctttaa tgaatggggt ctgcaaatta ttgccgcaat
     1501 tgataataat ccagcattaa ttaacactac aattaatgat aaacaaattc atggagttga
     1561 tgatctagat aaaatcatta aagagtacaa cgtacaaatc gctattatca ccgtaccaaa
     1621 agaatatgct caagagttag tcgatcacgc tgttgcggcg ggtgttaaag caatttggaa
     1681 ctttgctcct acacatataa gtgtgccaaa agatgttgtc ttacaaaatg agaatatggc
     1741 aagtagtctc agtcttttaa attacaattt aaatttaaaa acatcaaaac aaaaaaggaa
     1801 aacaaaacgg aaataataag gaaaagggct gcaaattgca gcccttttaa taaaatttaa
     1861 gctttaatcg tttttgcttt ctaacacttg tgttaactct tttaatttac gtttaagtgg
     1921 agttataaca cgccgtacat tcccatcttc aaacggatta cgaagtttag ctttcgctag
     1981 taattccaca aatggataac gaccaccaag gcggcaaagt ttcacatact tattccatgc
     2041 acgatcacga ttcttccaca tttctgttgc aaattggaaa gcaacgactt gtgctaaagt
     2101 gtagtcaata tagtaaaacg gactactaaa gatatgactt tgacggagcc agaatgtacc
     2161 tttttctagg aaaggagcgc ccttgtaatt acgaattggt aaatatttct tttcaatttc
     2221 ccgccacatg gcacaacgct cttcatgagt catctctgga ttagcgtata cgccttcttg
     2281 gaattcatca acggcaacgc catagggcaa gaatttaatc gtaccttcta agtgtgacaa
     2341 gcgatattta tcagcgtcat cgaacatact ttccatccat ggccaagcaa aaaattccat
     2401 gctcatgcta tgaatttcgc aagcttcgag tgttggttga cgatattcac taactttaat
     2461 atgacgactt tcgtacccct gataagcgtg tcctacttca tgcgttaaag tgtcgacgtc
     2521 accagctgtt ccgttaaaat tagcaaagat aaatggtgct tggtaacgcg ggaagtaagt
     2581 catgtaacca ccacctcttt taccagggcg agcatctaaa tctaaaagtt ctttatcaac
     2641 cataaaccgg aagaattctc cggtttcagg actaagttca tcatacattt tagttgcggc
     2701 tttcactaaa tatttactgt cacctagtgg cactgggtta ccatcgctaa agctttggtt
     2761 taagtcataa tgacgtggtg acttaatacc tgcgcggcgt aaacttgtgc gatataattt
     2821 atgtgcaagt gggacgactt cacgataaat ttggtcacga tagcctgcga cttctttagc
     2881 ggtatagtca agccggccta attttaagta accaagttca ataaagtttt tataaccaag
     2941 tttaactgcc attgcatggc gaacttgcac aagttcgtga taaatgcgac caagttcatc
     3001 attgttttct tcaaagaatt taacaacagc ggcactagcg cgttttctta catcacgatt
     3061 cttatcttga gtaaatttac ccatctgcgg gatattgtat acttcaccat caaacttaat
     3121 ttgtgcactg gcaataagct ttgaatattc attcgtgagt tgtgattctt tttgtaataa
     3181 ttcaatgatt gaaggatcaa atgttttaag ttgagtttca atcatcttga ataagtaact
     3241 tccccacttc ttttcaagtt cagcgcggaa gggagaagca acgagcagtt tattgtactc
     3301 attcattaat gcacttaaat acgggaaatg atgatcgaca tattcttggg ctttcacata
     3361 ttcttcattg cgtgaatctt gcgagaattt aacactaata ataacagcat cagtgacaaa
     3421 gtcatcaaca tacttagaaa tcttgcgcat aacacgagat gccgcatttg tgtctttagc
     3481 atttttaaat tgttcaatat actttgttaa ttgcttttga atttttggta cgtcagggac
     3541 gttaaatgga tattgactaa atttcatatt tgttttcctt tctaattttt aattattaaa
     3601 cttggttcag tcatttcact aggcgggact tccccaagta attcaatgat tgttggggca
     3661 atattactaa gtttgccacc ttctttcata agtttgatat ctttacgatt aatcccaaag
     3721 cgtactggat tactcgtatg agcggtaaat ggtttacctt cttcgtcaag taatttttcc
     3781 gagttaccgt ggtcagctgt aatcataatc gttgcatcgt gtttttccac ccattcaagg
     3841 atgcgcccaa cacatgcgtc cacagtttca agtgctttaa tcactgcact ttcaattgca
     3901 gtatgaccga ccatatcagg gtttgcataa tttaatataa caacatcaag atcaccttta
     3961 tctaattctt taagtaaagc atccgtgact cctggcgctg acatttctgg ttgtaagtca
     4021 taagtcgcta ctttggggga cggcactagg atacgacgcg cacctttaag ttcaggcttt
     4081 tcaacaccgt cataattaac cgtaccatct aagaaaaatg taacatgcgc atatttttct
     4141 gtttcagcga ttcgtagttg tgtgtaacct ttatccgcta aaaagacacc taagccatta
     4201 tcaagtttag gtagtgataa agcgatatgt ccatgaactg aatcggcata tttcatcgta
     4261 catgcgtaat aaatattttt taagattggg cttggcacaa atgccttata ttctggtttt
     4321 ccatctttta gtggtgggtt ttgatagaaa tatggattag tgacaatcgt tccaaattga
     4381 atcgcacgat ctggacggaa attaaagaaa ataacagcgt cattatctcc taattggcca
     4441 ttgacatttt tattaattcc gggaactaaa aattcatcac ttggatcaaa gccgttttta
     4501 ggtaatgttt cgtattgttc tttgaaatat tctctaaaat cactaaagaa taagccctta
     4561 cgattcacaa tcgcatcgta atggacattc gtacgattta aattcttatc acgatccatt
     4621 ccgtaataac ggccaccgat ggtagcgagt ttaccaaaat taattttcgc catataatcc
     4681 actaattctt ggatatattc tgggccagct tggggcgcta catcgcgtcc atccattaaa
     4741 gcgtgtaaat aaacctcttt agcgccatgc atttttgcta aatctaaaaa ggctttaatg
     4801 tgattaatat gtgaatgaac accgccatca ctaactaaac ccattaaatg taatttagaa
     4861 ttgtatttaa gtgtatgttt aacggcatta acaaatactt cattttcaaa aaagcgacca
     4921 tctttaatta gttgattaat ataagttagt gattgataaa caatgcggcc tgcaccaata
     4981 ttttggtgac cgacttcact atttcccatt tgcccagcgg gtaaacctac cgcttcgcct
     5041 gaggcttcaa ttaggacggt cggattattt ttatagaatg cgttaatgtt tggggttttt
     5101 gcttttttaa cggcattccc ttttgtttct tcgctatagc cgtagccatc taagattgta
     5161 agtataatag gacgtttatt cataataaaa aacctctttc cgatagtaat tatattacta
     5221 taaagggtaa ttgtaagcaa aaatgcttat ttaactagat tttagtaagt ctttttaatc
     5281 gtaaaataac gacaaacaat gaaaatataa cagaatcaac attaacatta atgttatcca
     5341 ctagtttgtt atgacgatat atttcattac gaataagtac aacattctca ctgagacctt
     5401 gtagtagttg cgctgtttgt ggagttgtat ctttcttttt cgctgctaac tcttgataag
     5461 cgcgctcttt ttccgccgct agcttattaa gttcggtaaa ttcacgtttt tccaaaagtt
     5521 cattcattac ttgtgcttca ctatttggtg actctaattc tttaccaatc attgcaaaaa
     5581 gggcaatttg acctgcaaaa aggctgtgaa ttgtttcttt gctctttttt aattgtcgtg
     5641 tgtagtcgag tgaaatatgc ataaaaacaa atgctaagag caagtatata acgactagaa
     5701 ccaaaataat aatacgcacg atttgcatag tttaattata acatacaacg attattgtaa
     5761 attagtgatt ttatttaaga ttaattatga aagcgttata ttaacttttt tcaagaataa
     5821 aaatagcaac ttaccaaaag aaagcatatt taaagaagtt tccattaaaa aaagataaaa
     5881 tatagctgta tacaaaagga ggaaattaat atatggaaaa tattgttaga atgccaattc
     5941 ttggcgaacg cttccccgag ttagatacaa tgacaacttt cgggaaaaag aaattaccta
     6001 atgattacga agggaaatgg tttattctct tctcacaccc aggtgacttc acaccagtct
     6061 gtacgactga atttattgct tttaatgaag aaattgaaga atttagaaaa atgaatgcgg
     6121 aacttatcgg actttcaatt gaccaacttc aatcacatat gaagtggact gaatttatcg
     6181 gtaagattac tggcaaagaa attggattcc caattatcgc tgacgaacta ggccgtgtcg
     6241 ctaatgcgct tggtatggtt caccctggta aaggcacaaa tactgttcgt gctgtcttca
     6301 tcgttgatcc aaaaggtgtt ttacgcttaa tgatgtacta cccacaagaa gttggccgtg
     6361 cagtttacga agtaaaaaga gcacttaatg cgctccaagt cgctgatgaa cataaagttg
     6421 ctgctccaga aaactaccca aataatgctt tcttaggcaa agacatggtc atcttaccgc
     6481 caccaggaac cgaaaaacaa aaagcagatc gtcttgcgtt acttgaacgc ggtgaagcaa
     6541 agagttatga ctggtggttc tcttataaaa aattaaaata agaattccta taactactaa
     6601 acaaggtgct acaaaacgca ccttgttttt gtttataata cttttcaacg atttagtggc
     6661 tatttataag tccttattcg ttttgacaat cactctattg agtgatatta tgttataaat
     6721 ggaggttttt caacatgggt acagctaaac caattattgc tattatctat gactttgaca
     6781 aaacattaac caccacagat atgcaaaact ataattttat tcctgcgctt ggtatgacac
     6841 cagaagaatt ttggggtgaa acaggaaaat ttagtgataa aactggcgtt gaacggattt
     6901 tatcatatat gtatatgatg attgtcaaag ctcgtgaaaa aggtattaat ttaacgaaag
     6961 aatttcttta tgaacaaggc aaaaacatgg aatacttcga tggggtctta acttggtttg
     7021 accgcattaa tgaatatggt gagaaaaaaa gcattaaaat tgaacactat ctcattagca
     7081 gcggcacaaa agaaatcatt gaaggttcag aaattgctaa acactttaaa gccatttttg
     7141 gttgtgaatt ttactttgat ccacaaacta aattaccagt atggccaaaa ctagcgatta
     7201 actacaccgc taaaacacag ttctacttcc gcattactaa aggtatctta gatgtcagcg
     7261 aagataatgc cttaaaccgt aaactagtag accatcgcat cccacatcgc aacattattt
     7321 atatcggcga cgggatgaca gatgttccgg taatgctcct tgttaaacaa aatggcggtc
     7381 actcaattgc tgtttatcca aagggtgaac gcgaaaaagt tgagtactta tacactgatg
     7441 gccgggtaaa ctttgttgct cgcgctgatt atacagaaaa aagtgatatt aattccattg
     7501 ttcgcttaat tattgaccaa gtgtctatcg ccgaacaaat tgaaaagaag aaacaacttc
     7561 ttaactagtt atgaacattt acgaactaaa tgaaattgtc gaaggcacta ttactgaaat
     7621 tagaccatat ggtgccatta ttacctttcc tgaaaatagg actgggcttt tacatataaa
     7681 acaaattagt gatgcctaca ttagtaacat caacacatac ttgcaacttg atgcacaaat
     7741 taaagtgcgg ataattgaaa ttgatgaaca taatggtttc ttaaaacttt cacttaaatc
     7801 cgttccttac aatgaacgca ttatttttga acgcgataaa aaagcaacaa acattaatcc
     7861 ggcacaaact gattttagtg cacttgaaaa agcattgccg ctttggattg aaaaggctat
     7921 taaggaggcg caaaatgatt agattaaatt atgacaatgt attaactacg attgattttg
     7981 aaaaataccg ctctaaactt gaaaaaatta atgaaggcat taatcaaaaa aagggagcag
     8041 gaaatgatta tcttggttgg gctgattggc cattacatta cgataaaaaa gaatttaatt
     8101 taattaaaga aacagcctta atgattagag aaacctttga tactttagtc gttactggta
     8161 ttggtggttc ttacttgggg gcaagagctg caattgaagc aattaacgga ttattgccgc
     8221 aaaaaggacc tgaaattatc tttttgggtc atactttttc tccgacctta acagcgcaaa
     8281 cgctcgaata cttaaaaaca aaaaactttg cgattaatgt tatttctaaa agtggtacca
     8341 ctactgaaac aagtattgct tttcgcttat taaagcaact attaatcgaa aaagtcggtg
     8401 aagcaaatgc taataaggca atttttgcga ctacagacgc taaaactggt gcgctccgta
     8461 aactagcgac acaaaatggc tacactactt ttacattgcc aagcgatatc ggtggtcgtt
     8521 atagtgtttt aaccgctgtt ggacttctac cacttgctgt tgcgggaatt gatattgatg
     8581 acttaatggc gggagctctt aaagcacgca gcgaaagcgt cgataattta gatcatgacc
     8641 tttataaata tgcagttatt cgcgacacac tctatcgtca aaattacaag agcgagctct
     8701 atgttgttta cgaaccacaa tacgctatgt ttaatgagtg gttaaaacaa ctatatggcg
     8761 aaagcgaagg taaagaaaag aaaggtttat tcccaacttc agttaccttc tccactgatt
     8821 tacatagctt aggacaattt attcaagacg gatctcctat tttatttgag acaacgctct
     8881 ttgttgaaaa tccaaaccaa gatgtcgtca ttcctagtga aaaagaaaac ttagatgaat
     8941 taaattacct tgcgggaaaa aaccttgccc ttgtcaataa gcaagctttt aaaggcacac
     9001 tcgccgccca tgttgaagat ggtaaggttc ctaatcttgt ctttgaaatt gctacacttg
     9061 atgcccacac tcttggttat atgttctact tctttatgcg ctcatgtgcc gtaagtgctt
     9121 atctacttga tataaatcct tttaatcaac caggcgtcga agtttataaa aagaatatgt
     9181 ttgccctact tggtaaacct agcagaaaat aataacaggc cgttttgata gtattatttg
     9241 ttgactaaat aactatctaa tgatatatta agtaagcata aattagcgac cagtcccgga
     9301 tggttagctc agctgggaga gcatcgcctt tacacggcgg aggtcggggg ttcaaacccc
     9361 tcaccatcca ccatttattt tatgccaaaa gtcgggagag tagcgaagtg gtcaaacgcg
     9421 gctgactgta aatcagttcc tgagggttcg gtggttcaaa tccgccctct cccaccatct
     9481 tttgtttggg gtatagccaa gcggtaaggc aacagacttt gactctgtca tctccctagt
     9541 tcgaatctag gtaccccagc cattcctccc gttagctcag gcggtagagc atttgacttt
     9601 taatcaaagg gtcgtaagtt cgattcttac acgggagacc atcgcccggg tggcggaaca
     9661 ggtagacgca agggacttaa aatccctcgg ttgttgagac cgtgccggtt caagtccggc
     9721 cccgggcacc agttccaaaa aaattgtcct actacggtag ggctttttta ttcataagca
     9781 ttctttttag taaatataat ttcaatttgt tatactattt gcatggaaga tttaaaacaa
     9841 actgaaaaca agattaaagt actacttaac cgcatcagac cttatattca aagtcacggt
     9901 ggtgattttg aatttgtaag atttgaagaa ggttttgttt atttaaagat gcttggcgct
     9961 tgcgaaggct gcccacttgt taatcaaacg ctagaatatg gtatcgaaga aatattgatg
    10021 gaagaggtac caggcgttct tggcgtctta cttgatgaat aaaagctagt acaaaaagaa
    10081 aaggtgcact taagatgcac cttttttaca ttattcctta ctatttataa taaagttttg
    10141 tgtcacttgt cttaaaatct actgctatca gtgtagtcta acagccactt aaggagaaaa
    10201 gtatccctac aaatagtacg cgttttataa agcctctttt acttgcctaa ataataatcc
    10261 actgctattt caatatgcgg cgaccatatc accacacaat tggtatcatc ttcataataa
    10321 ttaggataga aaagcgcctt gaatcgtaag tcatgctttc cgacttcaaa attagcgcct
    10381 aaagtccatg tttccgtgta tgtttcatta tttaagaaag taactgtttc aggaaattca
    10441 ctaggctcaa taaaaagtgg gtattgccat ctaggggcaa agggccaaaa tggtgaccaa
    10501 aaatatgaca tttcaatatc tgcgccacta ttatttttta atttaacttc aacactaaaa
    10561 tcttcaccgc tttttttaac aaccatctct gttgatatac taaattcata atcatcattt
    10621 attaaaagag catcaatatc cgcttttgta ttttttacaa tactgtcaat ttctttttta
    10681 ttccttgctt tattaatagc ttcttcccct ttttgtacag ctatatttaa agcttcttga
    10741 tgatcattaa agatatgctg ccaatcagtg acgaaacgcg ctatttcagc tttagcattt
    10801 tcgcgatatt cgttaagacc gctattacac gcagttagac taaaaagcat tggaattata
    10861 agaataagta aacttccttt tcgtttcata agatgtcaca gccttttatt ttagttataa
    10921 tttgcggcaa tattaaagtt ttatctctac ccttatttta ttacaaggtg gggaatttac
    10981 aatacgaaaa aagctataaa gtattctaat aaaattaatt tatctattta aatagtgtaa
    11041 gggtctattt caccgttagt gcggaaatat tcagcaatta attcacgaac atattcacca
    11101 gtaacagtat gtgggtaatc acgaatattt gaatacttgc tatcactaac ataatcgata
    11161 gtaacgattg tatattcttg atcattttga atatcaaatt taccgattgg ataagaagca
    11221 ttgctaccaa tgaaacttga agcagttgag cctaaaacat cttcaaacat cttaatttca
    11281 ttatcaaacg ggaaggcttt ataaatatcg ccatatgtta cttctcctgc atcaatcccg
    11341 tctactcgca caccaccata attatggaca gcaataattg tatcagggtt atgcgtaata
    11401 ctcgcgaagt tatacatcac cttattagcg agttttacaa gtgtactctt attaatataa
    11461 tttgtcagtg aacctataac ttcacctttt actctttcaa tttcttcagc atacttttca
    11521 ttaaataagt ttaataattc agtgtcttct gtgtaatctc ttttaatgtc cgctgcttcg
    11581 gtaaattcac ggctagtaac tttaaattct tttaagttaa agtcataata gccatgcact
    11641 tccattactt gtttaccatt accttccgtt tgcataattg gcacatcatt aataaactga
    11701 ttatcacgca catgatggtg accggaaaaa ataatatcca cgactttatt ttcgataata
    11761 ggcgcataag caccaagact attagcgagt gagtcatgcg ttgataagac aatcatatct
    11821 gcgccttgtg ctcgtaaatt ttctgcttcg cgttcaatat aagttgttac tggtttaaat
    11881 gtgtaagggg caatagcacc tgctaaaatg gagccttcaa gcatcccaat cgtcccaata
    11941 atcccaatgt taacatcgtt tcttgttacc atcgtactag ctttaaaagt ttcatcgtat
    12001 gacactaaat tcgtggcaag ctcaccggtt tcacgcacca tgatattagc gcctaaaaac
    12061 ggataattag cgagttcctt attcgtttca ataacagtgt cataccaatc aaattcgtgg
    12121 ttaccaatag tttgcgcatc aagctttaaa acattcattg cttcaactaa aaacttacca
    12181 cgattaatat ttgaatcagc actgccttgc cagatgtcac cagcatcaac atacactgtt
    12241 ttatttcttt tagcagcgaa ttcacttttt aaatatgaac caagtttaaa aataccaggc
    12301 tcactatttg tttcaacaat tgacccatga aaatcattag tagcgtaaaa acttaattcc
    12361 ttttgcccag ttctaccttc agtgcttgta attgtagtgc tagttgtact agtggtagtc
    12421 gtgctagtag gttcttcact acctaaggta ggtctagaat tgtcacttag agattcactt
    12481 cctctttcac atccgcttac tacaagcgta aaaataagga gcggtatcat taaaataatt
    12541 gtttttttct tcataatcgt atattttcct tccgattaac gattaaataa ccgttacgat
    12601 tgtgtatatc agctttatta taagtaaact tcttaccatc aggtccaata aaaataccgc
    12661 cagcttcagt cacaatgata tccgctgcag cgatatccca ttctttagtg cctggaccac
    12721 tacgataaaa taattcaatt tcacctttag caatcttaat cgctttaagt gtggagccgt
    12781 atgttattac attactaatt aaatctttat gtttttcgat atattcatta tcagcaggtt
    12841 taaaatgatg acgagaacga gtcgcggtta agttactagt tttatcatta acacttaact
    12901 tctttgtcga accatcagcg aatagttcgt aggctcctcc acctttagta gcgtaatata
    12961 tttcgttaag taccggcaca acaatgacac cgacaacaac ttcaccttta taagttaaag
    13021 cgatacacac agtaaattca ccatcacgat gaataaaatc ttttgtgccg tcgaccggat
    13081 caataatcca cacataatcc gtggtaagcc gagataaatc atcatcactt tcttcagtta
    13141 aaaaagcata atcaggaaaa agtggtccaa gatactcacg cattgcttta tccgccattt
    13201 tatcggctaa agtaacagga gaattatcat ctttaatttc aacatcaaag tcactttcat
    13261 aaatttttaa aacaatatca cgcgctattt tcccggcttc aatcgccgca tttaattcct
    13321 ttttatactt catcttttaa ctcctcagct ttagtaagtt gttgcaaaac gtcggcaatt
    13381 cgtgcttcat tttctaagcg acagccactt gcttttaagt gaccaccgcc accaaaaagc
    13441 gtagcgacct tttgcacatt ataattttta ctacgtaatt caacaaacac acgcccgtct
    13501 ttttcgataa aaaaagttgc ccataacgga tagtcatcga gatttgaata agtattgaca
    13561 taattactac cttcacttgg taatatgtct aattcttgta gttttaagta tgggacaata
    13621 atataaatta cattttcata agtttcataa tttgataaaa agtgtccgcg ttttttaaag
    13681 tcagcgagtg aacgcttcgc taaattctca ttaattaacg ctaaatccgc accaatatta
    13741 gtgagtcgcg ccatcacact aaaagtgtta ccgcttgtta agtcgtatct aaaacgacct
    13801 gaatcggtaa taatcccaag cgctaaggca gtcgcaccaa gtttatttgt gcgaagcttt
    13861 tctttcatca ttaattcacc aataatttcg gcagtagcaa tacgctttgt taacactaac
    13921 tcttcatcag taaacttttc cacgaaaata tgatgatcaa ttttaaatga tgcttttgct
    13981 aatttaaagc gttgatcctc aactctctca ccgttagcga catcgacaat aatcgccgcg
    14041 ctttctttaa tgaagtcgtc acttacttca tctaactcgc ccacaataga aacaaagggt
    14101 tggacattac tgcccaaccc aaaaacttct ttatgcggat aacttgtttt tattaattca
    14161 cgcaagccaa taatactccc aactgcatcg ccatcaggat aaatgtgtga aaagattgca
    14221 atcttatcat gctcttcaat gaacttacgt aaatttttaa acacttatgc agctttccct
    14281 atacaaccaa agacgcggca cttttcgtta acaactttac gaatgccttt cattcccgga
    14341 ccaataattt tacgtggatc ataaacttta tcattattcg ctaaagtttc acggacaatc
    14401 tttgtccatt cttgttgaat ttcagtgttg acattaattt tagcggtacc acgttcaatc
    14461 gccatttgaa tttggaaatc aggaatacca ctgccaccat gtagcacaag tgggactccg
    14521 actaatttat taatatgatc catttcatca aaaccaagtt ttggttcgcc tttatacgga
    14581 ccatgtacac taccaagcgc tggcgctaag cagtcaattt ttgttttttg caccatttct
    14641 aaacattcgt ttggatcagc atataatgtt tcagcaataa cgtgttcttc ttgaccaccg
    14701 atgctaccaa gttctgattc aacactaacg ccgcgtgcat gtgcgtaatc aactacgcgt
    14761 ttagtaattt ctaaattttc tttaaatggg tgatgacttg cgtcaatcat cacggaagta
    14821 aagccagcat caattgcctt tttacatact tcttcactgc tgccgtgatc taagtggatg
    14881 gcaattggaa tcgtatagcc acgatcttta attaaggctt ttaccatttg cacgacgaca
    14941 ttataaccgc ccatgtattt agcggctcct tcactaactc ctaaaataac tggagcttga
    15001 agctcttgta cctcatcgag aattgcttgg gtccattcta agttattaat gttgaattga
    15061 ccaacagcgt acttctcttt gtttgctttg ataagcattt ctttcatact cactaatggc
    15121 ataaaaattc tcctttatct tttaaaatta tttattcttc gtcttcttcg tcaagtgtgc
    15181 ttcttacttt cgcttcatct gcttgacctg tttcatcatc aaagtaaagg atttctggtc
    15241 cttcgtcttc ttcttcctca tcacctgctt catcaacttc agtgtaaact tcgtttagtg
    15301 gtaaagcaat gtcatcaaat aagacatttt cacgtaatgc ccacatattg tcgcctaaat
    15361 taacaaagcg accatccatc gttaaggcag tgtagaagcg tgaaattaaa tcatgacctt
    15421 cagcttcact atattcatgt tcgttaacaa caccttgcca taaatcataa aatgatagtg
    15481 attcaccgtg ttccttaagt agggtgtaag caatttcaat gtttgtcctt ttcttcatat
    15541 atcttcttgt attatctaat catgccccgg tacttcgatt aggtaataca tctccttt
//
LOCUS       DAOSTA010000021        14931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_4094_length_14931_cov_11.918056, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000021 DAOSTA010000000
VERSION     DAOSTA010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 14931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14931
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4094_length_14931_cov_11.918056"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1442
                     /locus_tag="PLU08_03110"
     CDS             192..1442
                     /locus_tag="PLU08_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage repair protein"
                     /protein_id="HOQ70715.1"
                     /translation="MEKVYAVIDLKSFYACVECAERGLDPFTTPLVVCDERRGDGTII
                     MAVTPYLKAKGLSNVMRKFELPRGIPYIFAKPRMELYVQKSTEFNALLLNYFAKEDWY
                     PYSIDETFIHLTPYLELYKKTPEELIEFIIKEIYDHLKLYVTVGIAPNMFLAKCALDL
                     EAKKDPRYYARWTMEDVKTKLWQIKPLHKIWGIGKRYERRLHKLGLFTMGDIAKCPKA
                     KLKEEFGIMGEELWEHANGIDNTDLSEKYVPLDKSLTSGQVLFRDYEVNEAPVLIREM
                     TDELCMRMRKNNLKAKKVGLSIMTSKPESSTYSKSMSLLLPTCSNTELFRAFMEVFNK
                     FPLEGAKIRSLHLSVSGLSSDPYEQLSLIEDYVTQNTKEQRMWETLDELHARYGKDKI
                     MRANALTRASTYLRRTKQIGGHNK"
     gene            1439..1744
                     /locus_tag="PLU08_03115"
     CDS             1439..1744
                     /locus_tag="PLU08_03115"
                     /inference="COORDINATES: protein motif:HMM:NF020438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HOQ70716.1"
                     /translation="MSRGMKKFLPFASLKEQALFLAEMQQQKKRVEKPTLSEDQCAEI
                     NRVLSEYTGETVLIYYYNCGFIKTHQAKIKKIDTLYKRLVFTDLTVSFTNLLHIEII"
     gene            complement(1745..3082)
                     /locus_tag="PLU08_03120"
     CDS             complement(1745..3082)
                     /locus_tag="PLU08_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70717.1"
                     /translation="MAKNKRKCRPLCFILKAVFVLLGVVVLVLGGYIGYVSLSYYRIG
                     DTSLQVLNNQTAKLSVADIGTKEFKITTYNVGFGAYDRDYSFFMDTSVFKDEYIHSQG
                     NKSNTGDRARGTSKAQVLRNINGAYDTIAALGDLDFMLYQEVDTSSTKSYKVNQYEIG
                     NKKHVDYATIYGINYHSAYLMYPLHEPIGKSNSGISTYSKYHIAKAERREFEITDAFF
                     GKFFDLDRAFTISEIPIEGSVKKLYVFNVHMSAYDKGGVIRKVQLEQLKAAIRVARDY
                     DGDNNYVVVGGDFNHDLVLDNPLVNEEYKANIFNKQETEILEANWYNYFRLDSGKHGT
                     RVPNIITGLEEDYTHDFYDMALSSHGPVNIATCRDASIKFIDKNNNGIVDNAMVTIDG
                     FLVSDNIAVAEIKTIGSGPGLQKENLPESDPRHGLGFVYSDHNPVLMSFKLQA"
     gene            complement(3082..4308)
                     /locus_tag="PLU08_03125"
     CDS             complement(3082..4308)
                     /locus_tag="PLU08_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOQ70718.1"
                     /translation="MKKSDLKKYARLIVRVGANVQKGQDVIITAELEQREFVLMLIEE
                     AYRAKANRVTLDWIDDDISKLHNKYRTVKSMSHFEKWELERYQYYVDKNPVRIYITSE
                     DPDGEKGINQEKRAQVMQNIYPVIKPYRDEMENKYQWTIAGAAGTKWAKKLFPDLTAK
                     QARNKLWDAILTTSRAYGDPIANWNEHNRSMSEKSRKLNELGLISLHYTSKNGTNFKV
                     GLIPNALFLAGGETTLGSNIFYNPNIPTEECFTTPKRGEAEGKVVATMPLSYQGELID
                     GFWFEFKDGKVVDFGAKQNEELLKQMLDMDEGARYLGEVALVPKESPINQLGLLFYST
                     LYDENASCHLALGLGFSNCIRDFEKYSLEELREMGINNSMIHVDFMIGVDDLRIVGTT
                     KYGQEVVIFNEGTWAI"
     gene            complement(4313..4909)
                     /locus_tag="PLU08_03130"
     CDS             complement(4313..4909)
                     /locus_tag="PLU08_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70719.1"
                     /translation="MEFKIENSVVYGLERAVKASGNPMRTEIEGGALHEKDIKRAVLL
                     GTTKGGEGHDNFLKGILVHVDITAPFYWWKEAQRYHWFDFISSQSTMHMLLKFKIDER
                     CVPETDKRVFPILEELVNDYNAISEEDKDAKVKAWRKIVATLPSGFCLAATMTTNYQQ
                     LKTMYFQRRNHKLKEWHTFCDWCLTLPHFKEFVIKEGK"
     gene            complement(4899..5696)
                     /locus_tag="PLU08_03135"
     CDS             complement(4899..5696)
                     /locus_tag="PLU08_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOQ70720.1"
                     /translation="MDIKTYVLKRKQNLKERILKDGGTPSLVIIQVNDDFASNKYVAG
                     KINDAREVGISAKLIKMPETTRLDELLKKIKKLNKDPHVNGIIVQLPLPKHIDENIVK
                     EAINPAKDVDGFHPLSRFKACTPLGIINYLKDEGVEIRGKNVVIIGRSNIVGKPAAML
                     FLEEDGNVTVLHSKTSREDLKNYVANADIMVVAVGRKHFINDEHTLKKSAVLVDVGIN
                     RDEEGKLTGDIKPGRDVYLQTPVPGGVGLLTRLTLLENVMEAYLHGI"
     gene            complement(5853..6284)
                     /locus_tag="PLU08_03140"
     CDS             complement(5853..6284)
                     /locus_tag="PLU08_03140"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco47II family restriction endonuclease"
                     /protein_id="HOQ70721.1"
                     /translation="MNSKSAEQTYRMMHNHIKENPNDKCFLVEVIAQQSRNIVWSVKL
                     DNEPCYDERIRRVSIDEFYKIVTGEENAFYKFCVQLPKTIKKLLANRKDNVKGTNTVI
                     TELQNRGGNLQKAIFSLAFANYCGFTTVEDQIDDLLNGNHH"
     gene            complement(6281..6667)
                     /locus_tag="PLU08_03145"
     CDS             complement(6281..6667)
                     /locus_tag="PLU08_03145"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco47II family restriction endonuclease"
                     /protein_id="HOQ70722.1"
                     /translation="MTIDEKVNDDYTLTFITQENFEKHVNELIDKYYEILSDYDLKRF
                     NSNLIDPIKLSIDKYLLDRTWKEIIDTEINRQRDKTITNALGDFHQNIFKYIDRCEVP
                     KTGFDIIYTNEAGQKIYVELKINIIQ"
     gene            complement(6667..7890)
                     /gene="dcm"
                     /locus_tag="PLU08_03150"
     CDS             complement(6667..7890)
                     /gene="dcm"
                     /locus_tag="PLU08_03150"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HOQ70723.1"
                     /translation="MIQIDYPKSLHKLRRTLNVSQQQLANILNVSFSSVNRWENGHYE
                     PTIIAKVKLDELFDQVKIKPIAKQEKYKSIELFAGAGGLALGLEQAGFDHIGLVEFEK
                     NAVDTLKINRPEWNVLLEDIREVCERDLEQEFNIKKGELDLISGGAPCQSFSYAGNRL
                     GLEDVRGTMFYYYATFLRKLQPKLFLFENVRGLLSHDKGRTYKTILDIFEEQGYITLY
                     EVLNAWDYSVPQKRERLITIGIRKDLFEEGIFEFPKKHKYKPTIKDVKLDVNPDKEHC
                     KRYSKYKEEIFKLVPPGGYWRDIEPEIAKEYMKSCWDMGGGRTGILRRLSLDEPSLTV
                     LTSPFMKQTDRCHPLEVRPFSYRENARFQTFPDDWVFSGTLSSKYKQIGNAVPVNLAK
                     EIGLELIKTLERINE"
     gene            complement(8094..8321)
                     /gene="secG"
                     /locus_tag="PLU08_03155"
     CDS             complement(8094..8321)
                     /gene="secG"
                     /locus_tag="PLU08_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOQ70724.1"
                     /translation="MGTLDIIFIIVSILLIIISLMQGGKSEGASGAITGGGLNIFVKT
                     KERGSEKVISYITYGLAILFFLLAILVKALG"
     gene            complement(8399..10387)
                     /gene="uvrB"
                     /locus_tag="PLU08_03160"
     CDS             complement(8399..10387)
                     /gene="uvrB"
                     /locus_tag="PLU08_03160"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOQ70725.1"
                     /translation="MKKTKHKFVLETTFTPTGDQPTAIKELVAGLERGDKHQVLLGAT
                     GTGKTFTIANVIEKVQKPTLILVHNKTLAGQLYAEFKALFPNNRVEYFVSNFDFYQPE
                     AYVPSRDMYIEKEAVQNAEIEMLRSAAINSLLERNDTIIVASVAAIYGLSDPEEYSKL
                     SFNIVEGEVINRKEFLIKLVDAQYTRNDYELRAGTFRVRGDVIEIMLPISEDYLIQVD
                     TFDDTIEKIREIDVLTGEVRKTFNTYPIFPAYAYASSRERINAIVPKILSDLASRLSY
                     FADEGKLLEAERLEFRTRQDVDSLQEFGICPGIENYARYIDGRKAGEKPYTLMDYFPK
                     DYLLVIDESHVTLPQVRGMFNGDRSRKQNLVDYGFRLPSALDNRPLQFNEFEEMINQV
                     IYTSATPGDYELDKTGGVVVEQLIRPTGLVDPEVFIKPSVGQIDDLMYELTKVIERGE
                     RAMVVTLTIKMAEDLTHYLKEHDFKVVYLHNETKTLERSQIIYELRKGKYDVLVGINL
                     LREGLDIPEVSLVAIIDADKEGFLRSERSLIQVMGRASRNVNGKVIMYANNMTGSMER
                     AIAETKRRRDLQIAYNKKHGIVPTTIIKPLGEPLHNFGTERELERINKGLMSRKQLEK
                     YIREIRAKMNAAAKAYDFEKAIEYREILFELEGKSKKT"
     gene            complement(10368..11339)
                     /locus_tag="PLU08_03165"
     CDS             complement(10368..11339)
                     /locus_tag="PLU08_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOQ70726.1"
                     /translation="MAKQRIKDIFKEDWRTLLKNYSLIVVGSILLAFGTGVFLLPATL
                     NTGGLSGVGIIGEGLFGFDADLVVLVMTWAFFFISLLFLGWRFTIKSLVSSFVYPLAL
                     ILLMRLPAIATETVKLFGEGADVATKLIAGVFGGVFNGVGVALTFRGGGSTGGVDILV
                     VILNKYLRLSYSVMSFVIDGLIIAVGLFVLKDVVLSLIGILSAFVFAITLEFVFVGKK
                     HAMTASIISREKSAEINDFIQNEIGQGTTLVPVLGGYQKDEYMMVVVHFDRSNYARLI
                     NGIAKIDKKAFVSIEGSTVVLGGNFESLNPVKEGLVNIKKKDEKDKA"
     gene            complement(11332..12442)
                     /gene="prfB"
                     /locus_tag="PLU08_03170"
     CDS             complement(join(11332..12369,12371..12442))
                     /gene="prfB"
                     /locus_tag="PLU08_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_132025324.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOQ70727.1"
                     /translation="MEYKVALSKANELKRLLGKLSDSLDVASLKETIDKLTIEINNPD
                     FWNNQNKAKTIIKKLNAAKEKINIYSLVETTVESAFELLKDDTFAEDSEYLSDLAFEI
                     DNAQKEGELLINLLLFSGEYDENDATVSIHAGAGGTESHDWADMLYHMYARFCERQGF
                     SLKVVDYQAGNEAGIKSVTLIIEGNNAYGHLRSEKGVHRLVRISPFDANKRRHTSFAS
                     VNVVPIFDETGDIEINDSDLRVDTYRSGGAGGQNVNKVETAVRITHVPTGIVVSCQME
                     RSQLMNREIAMRMLKSELYQLELERQKAKVSAIEGEKKMIEWGSQIRSYTFAPYTLIK
                     DHRTNYEVGDIARVMDGDIAEFMYKYLEMEARSNG"
     gene            complement(12446..14872)
                     /gene="secA"
                     /locus_tag="PLU08_03175"
     CDS             complement(12446..14872)
                     /gene="secA"
                     /locus_tag="PLU08_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOQ70728.1"
                     /translation="MSFIEKIFRFEQRRLKKIEKTAKKVIAYEKTMEALTDDELKAKT
                     PEFRERLTNGETLDDLLPEAFAVAREAARRTLGQFPYPVQIMGAIVLHHGDVAEMRTG
                     EGKTLTATMAVYLNALSGKGVHVVTVNEYLAARDADWMGQIYRFLGLTVGVNLREKTA
                     REKREEYLCDITYTTNSEVGFDYLRDNMASSVDGRVLRGLEYAIVDEADSILIDESRT
                     PLIISGGAKVQASSYNVADRFVKTLRRDKHFVVDIKTKTCNLTDEGNKLAERMFGVNN
                     IYDPQYSDLVHRIHNALKANYVMSRDVEYMVDANREIQLIDQFTGRIMPGREYSDGLH
                     QAIQAKENVTIKEETVTMATITYQNFFRLYTKLSGMTGTAKTEEEEFRKIYNMRVIQI
                     PTNRPVIREDAVDRVYARKEDKLNALVEEVKERHAKGQPILIGTSSVESSEEVSELLT
                     ANGLKHEVLNAKNHAREADIVALAGNKGQITIATNMAGRGTDIKINDEVKALGGLAVL
                     GTERHEARRIDNQLRGRSGRQGDPGFSRFYVSFDDLLMRRFSSDMMLKLVEGMGNEAL
                     ENKMFTNAITSAQKRIEGQNFDIRKSLLDYDNVLAKQRSIVYKKRDSIIYAGDIKDLV
                     QEQFTLAGLYLAKKAVPETGKDTLVNGVMLKQIMEPNYLPEGSINPSAYDESPPEEIG
                     EDLMLLMYRRYQLRRREWSEELADRVEREITLRAIDRNWTRHIDTMAHLREGIHLRSY
                     ANTNPLQDYVNEGYKLFGEAMDIIAVETVLNLLNAKINVAPPVEEGQEGTEENKETAE
                     KENIKKEDKK"
BASE COUNT         4519 a         3061 c         2329 g         5022 t
ORIGIN      
        1 ctcgctacct catcgttgcg aacgatgcgc tctcccagat gagctacggc cccaagtcta
       61 actaaaagtt tatcaaacta gtaaataatt gcaagggcta tcgcttatca attttaatgg
      121 tgaaaatctt ttactaactt tgttcatcct tctgtctaaa aattaaagtg tcctcttgta
      181 taattatggt aatggaaaaa gtatatgccg ttatcgattt aaaaagtttc tacgcatgcg
      241 tagaatgtgc agagcgtggt cttgatccct ttaccactcc tttagtggtg tgcgatgagc
      301 ggcgcggaga cggcactatt attatggctg ttaccccgta cttaaaagca aaaggtttaa
      361 gtaatgtaat gcggaagttt gaacttccga gaggtatccc ttatatcttt gctaaaccgc
      421 gtatggaact atatgtacaa aaatcaactg aatttaatgc cctcctactt aattattttg
      481 caaaggaaga ctggtatcct tattcgattg atgaaacttt tattcattta acaccgtact
      541 tagaacttta taagaaaaca ccagaagaac ttattgaatt tattattaaa gaaatatatg
      601 atcacttaaa actctatgtc accgtcggta tagcgccaaa tatgttttta gctaaatgcg
      661 cacttgattt agaagccaaa aaagacccgc gctattacgc aagatggaca atggaagatg
      721 ttaaaactaa attgtggcaa attaaaccac tgcataaaat atggggtatt ggtaaacgct
      781 atgaaagaag actccataaa ttagggcttt ttacaatggg ggacattgct aagtgtccga
      841 aagcgaagtt aaaagaagaa tttgggatta tgggtgaaga gctttgggaa cacgctaacg
      901 ggattgataa caccgactta agtgaaaagt atgtcccact tgataagtca ctaacaagtg
      961 gacaagtgtt attccgtgat tacgaagtta acgaagcacc cgttttaatt cgtgaaatga
     1021 ctgatgaatt atgtatgcgc atgcgtaaaa ataatttaaa agctaaaaaa gttggtttaa
     1081 gtattatgac tagtaagcca gaaagtagta catacagtaa atcaatgtca cttcttcttc
     1141 caacatgctc caatactgaa ctatttagag ctttcatgga agtatttaat aagtttcctt
     1201 tagaaggtgc taaaatacgc tctcttcatc ttagcgtcag cggactttca agcgaccctt
     1261 atgagcaact tagtctcatt gaggattatg taacacaaaa cactaaagag caaagaatgt
     1321 gggaaacact tgatgaactt cacgcgcgtt acgggaaaga taaaataatg cgcgcgaacg
     1381 cactcacgcg tgcgagcact tatttacgac gcactaaaca aattggaggt cacaataaat
     1441 gagtcgcggg atgaaaaagt ttttaccgtt tgcaagtcta aaagaacagg cacttttcct
     1501 agcggaaatg cagcagcaaa aaaagcgtgt tgaaaagccc acactcagtg aagaccaatg
     1561 cgcagaaatt aatcgtgttt taagcgaata caccggcgaa accgtattaa tttattatta
     1621 taactgcggt tttattaaga cgcatcaagc caaaataaaa aagattgata ctttatataa
     1681 acgcttagtg tttactgacc taaccgtttc atttaccaat cttttacata ttgaaattat
     1741 ttaattacgc ttgtaattta aaactcataa gcacagggtt atgatcacta taaacaaacc
     1801 ctaacccgtg acgcgggtcg ctttcgggta agttttcttt ttggagacca gggccactac
     1861 caatcgtttt aatttcagct accgcgatat tatcactaac aaggaaacca tcaattgtga
     1921 ccattgcatt atcaacaatc ccgttattat ttttatcaat aaatttaatt gaggcgtcgc
     1981 gacatgtggc aatatttacc gggccgtgac tagatagcgc catatcataa aagtcatgcg
     2041 tataatcttc ttctaaaccg gtaataatgt ttgggactct tgttccgtgc tttcctgagt
     2101 caaggcgaaa atagttatac caattcgctt caagaatttc tgtttcttgt ttattaaaga
     2161 tatttgcttt atattcttcg ttaacaagcg ggttatcaag cactaagtca tgattaaagt
     2221 cgccaccaac gacaacatag ttattatctc catcataatc acgcgctact ctaattgccg
     2281 ctttaagttg ttcaagttgt actttacgaa tgacaccacc tttatcataa gcgctcatat
     2341 gcacattaaa tacataaagc tttttaactg aaccttcaat tggtatttca ctaatagtga
     2401 aagcacgatc taaatcaaag aatttaccaa agaaggcatc cgtaatttca aattcacggc
     2461 gttcagcttt agcgatatga tatttagagt atgtcgaaat acctgaattt gatttaccaa
     2521 ttggttcatg cagtgggtac attaaataag cagagtgata gttaataccg taaattgttg
     2581 cgtaatcaac gtgtttttta ttaccaattt catattgatt aactttataa gactttgttg
     2641 aacttgtgtc aacttcttga taaagcataa agtctaaatc acctaaagct gcaattgtgt
     2701 cataagcacc gttaatattc cttagcactt gtgctttact tgtaccacgc gcacgatcac
     2761 cagtgttact tttattacct tgcgaatgaa tatattcatc tttaaaaaca ctcgtgtcca
     2821 taaagaaact gtaatctctg tcatatgccc caaaaccaac attatatgtt gtaatcttaa
     2881 attcttttgt accaatatca gcgacactta atttcgctgt ttgattgttt aatacttgaa
     2941 gactagtgtc accaatgcgg tagtaagaga ggctaacata acctatataa ccacctaaga
     3001 caagcaccac gacacctaaa agcacaaaca ctgcctttaa gataaagcat aacggtctac
     3061 attttctttt attttttgcc attaaattgc ccaggttcct tcgttaaaga ttactacttc
     3121 ttgaccgtat tttgttgtac cgacaatccg taaatcatca acgccaatca taaaatcaac
     3181 atgaatcatt gagttattaa ttcccatttc acgaagttct tcaagagagt acttttcaaa
     3241 atcacgaata caattactaa acccaagacc tagcgctaag tggcacgaag cattttcatc
     3301 ataaagcgtt gaataaaata agagccctaa ttgattaatt ggtgattctt tcgggacgag
     3361 cgctacttca ccaagataac gagcgccttc atccatatct aacatttgtt ttaataattc
     3421 ttcattttgt ttagcgccaa agtcgacaac tttaccgtct ttaaactcaa accaaaagcc
     3481 atcaattaat tcaccttgat aactaagcgg catcgtcgct acgaccttgc cttcagcttc
     3541 tccgcgcttt ggtgttgtaa aacattcttc agtcggaata ttaggattat agaaaatatt
     3601 actacctaaa gttgtttcac cacccgctaa aaagagtgca ttaggaataa ggccaacttt
     3661 aaaattagtg ccgtttttgc tagtgtaatg tagtgaaatg agtcctaatt cgttaagctt
     3721 gcgagacttt tcgctcatac ttcgattatg ttcattccaa ttagcaatcg ggtcgccata
     3781 agcacggctc gtcgttaata ttgcgtccca taatttattg cgggcttgtt ttgcggttaa
     3841 gtctggaaat aatttcttcg cccactttgt accagcagca ccggcaattg tccattgata
     3901 cttattttcc atctcatcac gataaggttt aataacgggg taaatatttt gcattacttg
     3961 cgcacgcttt tcttgattaa tccctttttc accatcaggg tcttcgcttg taatataaat
     4021 tctaacggga ttcttatcaa cataatattg atagcgttct aactcccact tttcaaaatg
     4081 tgacattgat ttaacagtgc ggtatttatt atggagttta ctaatgtcgt catcaatcca
     4141 gtcaagcgtc acgcggtttg ctttagcgcg ataagcctct tcaataagca ttaacacaaa
     4201 ttcgcgttgt tcaagttccg cagttataat aacatcttgt cctttttgta cattcgctcc
     4261 gactctaaca ataagccgcg catatttttt aagatcgctt tttttcatac atttattttc
     4321 cttctttaat tacaaactct ttaaagtgtg gcaaagttaa acaccaatca caaaaagtat
     4381 gccactcttt aagtttgtga ttacgacgtt ggaagtacat cgttttaagt tgttgatagt
     4441 tagttgtcat tgtcgccgca agacaaaaac cgcttggtaa agtcgcaaca atcttacgcc
     4501 acgccttaac ttttgcatct ttatcttctt cacttatggc gttataatca tttactagtt
     4561 cttctaaaat agggaaaaca cgcttatcag tttcggggac acagcgttca tcaatcttaa
     4621 attttaacag catatgcatt gttgattgac tactaataaa gtcaaaccag tggtagcgtt
     4681 gtgcttcttt ccaccagtaa aaaggagcgg taatatcaac atgcactaag atccctttta
     4741 aaaagttatc atgaccttcg ccacctttag tcgttccgag tagcactgct cgcttgatat
     4801 ctttttcatg tagcgcgcca ccttcaattt cggtccgcat cggatttccg ctcgctttaa
     4861 cggcgcgctc taaaccgtag acaacactgt tttcaatttt aaattccatg taaataagcc
     4921 tccattacat tttctaataa agttaatcta gttaataacc caacgccacc tggcactgga
     4981 gtttgtaaat aaacatcacg ccctggttta atgtcaccag ttaattttcc ctcttcatca
     5041 cgattaatcc caacatctac aagaactgca ctctttttaa gtgtatgttc gtcattaata
     5101 aagtgtttac gccctacggc gacaaccata atatccgcat tagcgacata atttttaagg
     5161 tcttctctac ttgtctttga atgtaatact gttacattac cgtcttcttc taaaaagagc
     5221 atagcagcag gtttaccaac aatattgctg cgcccaataa tcaccacatt cttcccgcgg
     5281 atttcaacac cttcatcttt taaataatta atgataccta gtggcgtaca cgctttaaag
     5341 cgagaaagcg gatgaaagcc gtccacatct ttagcgggat taatagcttc tttaacgata
     5401 ttttcatcaa tatgttttgg cagcggtaat tgcacaataa tgccgttaac atgtggatct
     5461 ttattaagtt tttttatttt cttaagcaat tcatcaagtc ttgttgtttc gggcatttta
     5521 ataagctttg cgctgatgcc tacttcacgg gcgtcattaa tcttacctgc aacatattta
     5581 ttgctcgcaa aatcatcatt tacttgaatg ataactaaac ttggggtgcc cccatctttt
     5641 agtattcgtt cttttaaatt ttgtttacgt tttaaaacat aagttttaat atccataaat
     5701 gcacctcata aaataaatta taacacgaga gcaaaagcct agatatctaa gacaataaaa
     5761 aagatgagtt tgcacgatta agctaattct aaagactcta aaatttaata gtcgcttagt
     5821 aaattagttt taaattgttt gttacatatt tttcaatgat gattaccatt taataaatca
     5881 tcaatctgat cttcaactgt tgtaaatccg caatagtttg caaatgctaa ggaaaatatt
     5941 gctttttgta aatttccgcc tctattttgt aattctgtta tcaccgtgtt cgttcctttt
     6001 acattatctt tcctatttgc aagtaatttt tttattgttt ttggtagttg cacacaaaat
     6061 ttataaaagg cattttcttc acccgtaaca attttgtaaa attcgtcaat tgaaactcga
     6121 cgtatacgct catcatagca gggttcatta tctaatttaa cactccatac aatatttcta
     6181 ctttgttgtg ctataacttc aactaaaaaa catttatcat ttgggttttc ttttatgtgg
     6241 ttatgcatca ttcggtaagt ttgttccgct gattttgaat tcattgtatt atgtttattt
     6301 ttagttctac atatattttt tgtcccgctt cgttagtgta aataatatca aacccagttt
     6361 taggaacttc acaacggtca atatatttaa aaatattttg atgaaaatca ccaagagcat
     6421 tcgtaattgt tttatcacgt tgtctattaa tttcagtgtc gataatttct ttccaagttc
     6481 tgtctaataa atacttgtca attgataatt ttattggatc tataaggttt gaattgaaac
     6541 gttttaaatc ataatcacta agtatttcat agtatttatc aattaattca tttacatgtt
     6601 tttcaaaatt ttcttgagta ataaaagtta gagtatagtc atcattcacc ttttcgtcga
     6661 tagtcattat tcatttatcc tttcaagcgt tttaattaat tctaaaccta tttctttcgc
     6721 taagttgact ggtacagcat ttccaatttg tttatatttt gaactaagcg tgccagaaaa
     6781 aacccaatcg tcaggaaaag tttgaaatct agcattttca cgataggaaa aaggtctaac
     6841 ttctaaagga tgacatctgt cggtttgttt cataaatgga cttgtgagta cagtgagtga
     6901 tggctcatct aaacttagtc tacgtaatat tcctgttctt cctccaccca tatcccaaca
     6961 cgacttcata tactctttgg caatttctgg ttcaatatct cgccaatagc caccaggagg
     7021 aacaagttta aatatctctt ccttgtattt tgaatagcgt ttacaatgtt ctttatcagg
     7081 atttacatca agtttaacat ctttaatggt cggcttatat ttatgctttt tcgggaactc
     7141 gaaaattcct tcttcaaata aatcttttct aatcccaatc gtaattaatc tttctctttt
     7201 ttgtggaaca ctataatccc aagcatttaa cacctcataa agtgtaatat atccttgttc
     7261 ttcaaaaata tcgagaatag ttttatatgt tcttccttta tcatgggata ataaaccacg
     7321 tacattttca aataaaaata attttggttg taattttcgc agaaatgtcg cgtaataata
     7381 aaacatcgtt ccgcgtacat cttctaaccc aagtcgatta ccagcgtagc taaatgattg
     7441 acatggtgcc cctccggaaa ttaaatcaag ttcacctttt tttatattaa attcttgctc
     7501 taaatcacgt tcacacacct cgcgaatatc ttcaagaaga acattccatt ctggtctatt
     7561 aatttttaga gtatcaacag catttttctc aaattccact agaccaatat gatcaaaacc
     7621 tgcttgttca agcccaagcg ctaatccacc cgctcctgcg aataattcaa ttgacttata
     7681 tttttcttgt tttgcaattg gtttaatttt aacttgatca aataattcat caagttttac
     7741 ttttgcgata attgttggct cataatgacc attttcccac cggttaaccg atgaaaaaga
     7801 aacatttaaa atatttgcta attgttgttg tgaaacatta agtgttcttc ttaatttatg
     7861 aaggcttttt gggtaatcaa tttgtatcat ataacttatc tcgcttccaa aaataatata
     7921 gcactttatt tcacttttat aattttaata ctttccttat ttatttataa ataagttttt
     7981 gctgtggttt attacaacta ctaataaata caataaattt aataaaaact tctcaatatt
     8041 atacataata aaaaaacacc ctgccgggtg ttaatttaat agttttaata atcttaacct
     8101 aaagctttaa caaggattgc aaggagaaag aacaaaatag caaggccgta agtaatataa
     8161 gaaattactt tttcacttcc tctttctttt gttttgacga aaatattaag gccgccacca
     8221 gtgatagcgc cagatgcccc ttcactttta ccaccttgca taagcgagat aataataagt
     8281 aagattgata cgataatgaa gataatatct aatgttccca tagtcttcct ccagttcttt
     8341 attaatatac actatttata aattaattca agaaaacatt atttaaatat acatatattt
     8401 atgttttttt actctttccc tctaattcaa agagaatttc acgatattct atcgcttttt
     8461 caaagtcata ggctttagcg gcagcattca tcttagcgcg aatttcccga atatattttt
     8521 ctaattgttt acggctcatt aagcctttat taatccgctc aagttcacgc tctgtcccga
     8581 aattatggag tggttcgcca agtggtttga taattgtggt gggaacaatt ccgtgctttt
     8641 tattataagc aatttgtaaa tctcttcttc tttttgtttc cgcaatagcg cgttccattg
     8701 accccgtcat attgttcgca tacattatta ctttcccgtt aacatttcga ctcgcgcggc
     8761 ccattacttg aataagtgat cgctcactac gtaaaaatcc ctctttatcc gcgtcaataa
     8821 tcgccacaag tgaaacttca ggaatatcta gtccttcacg cagtaaatta atcccgacaa
     8881 gcacatcata tttccctttg cgtaattcat aaattatttg actgcgttcc agtgtctttg
     8941 tttcattatg gagatagaca actttaaagt catgctcttt taagtaatgg gttaggtctt
     9001 ccgccatttt aattgtaagc gtgacaacca ttgctctttc accacgttca ataactttcg
     9061 ttaattcata cattaaatca tcaatttgtc caacactcgg tttaataaat acttctggat
     9121 ccactaaacc tgttggccgg attagttgtt caacgactac tccacctgtt ttatctaact
     9181 cataatcacc aggtgttgcg cttgtgtaaa ttacttgatt aatcatctct tcaaattcat
     9241 taaattgtag cgggcgatta tctaaagcac tcggtaatct aaaaccgtag tccactagat
     9301 tttgtttacg actgcgatca ccattaaaca tccctcttac ctgaggtaaa gtaacatgac
     9361 tctcatcaat cacaagtaaa taatctttag gaaaataatc cattaaggta taaggctttt
     9421 ctccagcttt acgcccatca atataacgcg cataattttc aatcccagga caaatcccaa
     9481 attcttggag tgaatcaaca tcttgtcttg ttctaaattc cagccgttcg gcttcaagga
     9541 gtttaccttc atcggcaaag taacttaatc tacttgctaa gtcacttaat atttttggca
     9601 caatggcatt tatccgttca cgacttgaag cataggcata tgcgggaaaa attggataag
     9661 tgttaaatgt tttgcgcact tcaccagtta aaacatctat ttctctaatc ttttcaatag
     9721 tgtcgtcaaa tgtatcaact tgaattaaat agtcttcact gatagggagc attatttcaa
     9781 ttacgtcacc ccgtactcta aaagtaccag cgcgaagttc gtaatcatta cgtgtgtatt
     9841 gcgcatccac gagtttaatt aaaaactctt tgcgattaat cacttcacct tccacaatat
     9901 taaaggatag tttactatat tcttcagggt cacttaatcc ataaattgcc gcgacactag
     9961 cgacaataat tgtgtcatta cgctcgagta aactattaat agcagcagag cgaagcattt
    10021 caatttcggc attttgcact gcttcttttt caatatacat atcgcgtgat ggcacataag
    10081 cttctggttg ataaaaatca aagttagaca caaaatattc cactctatta ttaggaaaca
    10141 atgctttaaa ttcagcgtaa agttgccccg ctagtgtttt attatgcact aagatcagcg
    10201 ttggtttttg cactttttca ataacattag cgatggtaaa agttttacca gtgcctgttg
    10261 ctcctaaaag tacttgatgc ttatcaccgc gctctagccc agctactaac tctttaatcg
    10321 ccgttggttg atcaccagtg ggcgtaaaag tcgtttctaa aacaaattta tgctttgtct
    10381 ttttcatctt tctttttaat atttactaag ccttctttaa ctggatttaa actttcaaaa
    10441 ttaccgccta acactactgt tgacccttca atactcacaa aagctttttt atcgatttta
    10501 gcaataccgt ttattaaacg agcgtaatta ctacgatcaa aatggacaac aaccatcatg
    10561 tactcatctt tttgataacc acctaaaact ggcactaagg ttgtaccttg accaatttcg
    10621 ttttgaataa aatcattaat ttctgcactt ttttcacgac ttataatcga agcagtcatc
    10681 gcatgctttt taccaacgaa gacaaattca agagtaatcg caaacacaaa tgcactaaga
    10741 ataccgatta aagagagtac aacatctttt agcacgaata acccaacggc aataattaag
    10801 ccatcaatca caaaagacat aacactataa cttagtctta aatatttatt taaaattact
    10861 actaaaatat ccactccgcc cgttgaaccg ccacctctaa aagttaatgc cacaccaaca
    10921 ccattaaaca caccaccaaa gacaccggca ataagtttag tggcaacatc agcgccctca
    10981 ccaaacaatt taacggtttc agttgcaatt gctggtaaac gcatgagtaa aataagcgct
    11041 aaaggataaa caaatgaact aacaagcgat ttaattgtaa agcgccaacc taagaataat
    11101 aaactaataa agaagaaagc ccaagtcatc acaagtacga ctaaatcagc gtcaaaacca
    11161 aataagcctt caccaataat gccgacacca cttaaccccc cagtattaag tgttgcgggt
    11221 aaaagaaaaa caccagtacc aaacgctagt aaaattgatc caacaacaat taaactatag
    11281 tttttaagta aagttctcca atcctcttta aatatatctt ttattctttg tttagccatt
    11341 gcttcttgcc tccatttcaa ggtatttata cataaattcc gctatgtcac cgtccatcac
    11401 ccgtgcaata tcaccgactt cataatttgt gcggtgatct ttaataaggg tatagggtgc
    11461 aaaagtataa gatcttattt ggctacccca ttcaatcatc ttcttttcac cttcaatcgc
    11521 gcttaccttt gctttttgtc gttctaattc tagttgatat agttcacttt taagcatccg
    11581 catcgcaatt tcacgattca ttaattgact gcgttccatt tgacacgata caacgatacc
    11641 cgtcgggaca tgagtaatac gcactgcagt ctcaactttg ttaacatttt gaccgccagc
    11701 accaccactg cgataagtgt caacccttaa atcactatca ttaatttcaa tatcaccagt
    11761 ttcatcaaaa attggcacaa cattaacact cgcaaatgaa gtatgtcttc ttttattcgc
    11821 gtcaaaaggt gagatacgca ctaagcggtg tactcctttt tcactacgca aatgaccata
    11881 agcattatta ccttcaataa taagtgttac actttttatg ccggcttcgt tccctgcttg
    11941 gtaatcgaca actttaagcg aaaaaccttg tctttcacaa aagcgtgcat acatatgata
    12001 gagcatatcg gcccagtcgt gcgactccgt cccgcctgca ccagcgtgga tacttacggt
    12061 tgcatcattt tcatcatatt ccccgctgaa aagcagaaga tttataagta attcaccttc
    12121 tttttgcgca ttatcaattt caaatgctaa atcacttaaa tactcactat cctcggcaaa
    12181 agtgtcgtct tttagtagtt caaaggcact ttccactgtt gtttcaacta gtgaatatat
    12241 attaattttc tctttcgcag catttaattt tttaatgatt gttttagcct tattttgatt
    12301 attccaaaag tcaggattat ttatttcaat cgttaattta tcaattgttt cttttaaact
    12361 agcgacatca aagagagtca ctaagcttac ctagtaatct tttaagttca ttagctttac
    12421 ttaaggcaac tttgtactcc atcgtttatt ttttatcttc cttttttata ttttcctttt
    12481 cggctgtttc tttattttct tcagtgcctt cttgaccttc ttcaacaggt ggggcaacat
    12541 taatcttcgc gtttaataaa tttaaaactg tttcaacagc aataatatcc atcgcttcac
    12601 cgaatagctt ataaccttca ttaacatagt cttgaagtgg gttagtattt gcgtaactac
    12661 gtaagtgaat accttcgcgt aagtgcgcca ttgtgtcaat atgccgtgtc cagttgcggt
    12721 caattgcccg taaagtaatt tcacgttcaa cgcggtcggc aagttcttca ctccattcac
    12781 gtcttctaag ttgataacga cgatacatta aaagcattaa gtcttcacca atttcttctg
    12841 gtggtgattc gtcatacgct gaagggttaa tcgaaccttc tggtaaatag tttggttcca
    12901 taatttgttt taacattacc ccattaacaa gcgtatcttt accagtttct ggaacagctt
    12961 tttttgctaa ataaagaccc gcaagagtaa actgttcttg cactaagtct ttaatatcac
    13021 cagcgtatat tatgctgtca cgtttcttat aaacgataga gcgttgtttt gctaaaacat
    13081 tgtcataatc taataaactt ttacgaatat caaagttttg tccttcaatt cgtttttggg
    13141 cactagtaat agcgttagtg aacattttat tttctagtgc ttcgtttccc atcccttcga
    13201 cgagtttaag catcatatca ctagagaaac gacgcatgag taagtcatca aatgagacat
    13261 agaagcgtga aaacccaggg tcaccttgcc gtccactccg gccgcgtaat tggttatcaa
    13321 tacggcgagc ctcatgtcgc tctgtcccta ataccgcaag gccgccaagt gctttaactt
    13381 catcattaat tttaatgtca gtaccacgac cagccatgtt cgttgcaatt gtaatttggc
    13441 ctttattacc cgcaagcgca acaatgtctg cttcacgcgc gtggttttta gcgttaagta
    13501 cctcgtgttt gagtccgttt gctgttaaaa gttcactaac ttcttcactt gattcaacgc
    13561 tgcttgtccc aataagaatt ggttgtcctt tagcgtgacg ttctttaact tcttcgacaa
    13621 gtgcattgag tttatcttct ttacgtgcat atactcgatc gacagcgtct tcacgaataa
    13681 cagggcgatt cgtcgggatt tgaataacac gcatattgta aattttgcgg aattcttctt
    13741 cttcggtttt agcagtacct gtcatcccgc ttagtttagt gtaaaggcgg aagaagtttt
    13801 gataggtaat cgtcgccatc gtcactgttt cttctttaat cgtgacattt tctttcgctt
    13861 gaattgcttg atgcaaccca tcactatatt cacgaccggg cataatccgt ccagtaaatt
    13921 ggtcaataag ttgaatttca cgatttgcgt caaccatata ttcaacatca cgactcatga
    13981 cataatttgc tttaagcgca ttatgaatcc ggtgcactaa gtcactatat tgtgggtcat
    14041 aaatattatt aacaccaaac attctttccg cgagtttatt accttcgtcc gttaagttac
    14101 atgtttttgt tttaatatca acgacaaagt gcttatctct gcgtaatgtt ttaacgaaac
    14161 gatcggcgac attataacta gatgcttgta ctttagcgcc accagaaata atgagcggtg
    14221 tgcgtgattc gtcaattaaa atcgagtcag cttcgtcaac aatagcgtac tccaaacctc
    14281 gtaatacacg accatcaaca ctgctagcca tattgtcacg aagatagtca aaaccaactt
    14341 cactatttgt agtgtaagta atatcacata aatattcttc acgtttttca cgcgctgttt
    14401 tttcacgtag gttaacaccc acagttaaac caagaaagcg ataaatttga cccatccaat
    14461 cggcgtcacg agcggcgaga tattcgttaa cagtaacaac atgcacacct ttgcctgata
    14521 atgcattaag gtaaacggcc attgtcgcag ttaaagtttt accttcacca gttctcatct
    14581 ccgcaacatc accatgatgt aagacgattg cccccataat ttggacggga taagggaatt
    14641 gtcctaaagt tctacgtgct gcttcgcgtg ccacagcgaa ggcttctggt aataagtcat
    14701 caagcgtttc cccattagtg agtcgttctc taaattcggg tgttttagcc tttagttcat
    14761 catcagttaa agcttccatc gttttttcat aagcaataac ttttttggct gttttttcta
    14821 tttttttgag gcgcctttgt tcaaagcgga atattttttc tataaaactc atatacactt
    14881 gtattatcta atcatgcccc ggtacttcga ttaggtaata catctccttt c
//
LOCUS       DAOSTA010000022        13550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_4631_length_13550_cov_14.621119, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000022 DAOSTA010000000
VERSION     DAOSTA010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 13550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13550
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4631_length_13550_cov_14.621119"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1944
                     /locus_tag="PLU08_03180"
     CDS             <1..1944
                     /locus_tag="PLU08_03180"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOQ70729.1"
                     /translation="KIKYFGNEELSIPMEQIHLIRKYSGREGVAPRLHKIGSPVWQRT
                     KVEISKKIEDIAEELIAIQAARYQRPGFAFPPDDEFQKEFELGFPYELTYDQEIALRE
                     IKNDMESPLIMDRLLSGDVGFGKTEIALRAAFKAISAGKQVMFLCPTTLLARQHYEVS
                     LERFANFGVKIAMISRLVTQKEKDEIVKDFQAGNIHLLIGTHALFNIDVESVDLGLLI
                     VDEEQRFGVEQKEIIKKMKTRVDVLTLSATPIPRTLQMSLVGIRGLSQINTAPQERMP
                     IQTYVIKRDDYVIKELVERELGRGGQVFYLYNRIATIYHVANKLSRLVPQAKIGVVHG
                     KMDRADIEDVMTSFYNNEINVLVATSIIENGIDVPNANVIIVENADTFGLSQLYQIKG
                     RVGRGNRIAYAYLLYQGNKIMNDDAKKRLQAIQEFTDLGSGYKIAQRDLMIRGAGDIL
                     GAEQSGFIDSIGIDMYLKMLEDAINEKKYKLTSDPIEKTINISGLEAYIPDDFASKSE
                     KFDIYLEIKKSKKMKELKDFEEKVIDMYGKLPRSLEILFLKRQVELVVNSRANFIEDI
                     KDEDVFIDIIPGRAFLDIPGSVVMLNNEIGTTYQNIRFITQDDKIKLRIIKRGDWFHA
                     YASIVKAIVMIVRETMKNKDETR"
     gene            1982..2191
                     /locus_tag="PLU08_03185"
     CDS             1982..2191
                     /locus_tag="PLU08_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70730.1"
                     /translation="MAKDISTAGKVIVDKRTVKPSYTVKVDDVLELYLGAHIVVVKVL
                     TVDEKEIKSNPEKGYELLSRTKNAN"
     gene            2181..3407
                     /gene="tilS"
                     /locus_tag="PLU08_03190"
     CDS             2181..3407
                     /gene="tilS"
                     /locus_tag="PLU08_03190"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOQ70731.1"
                     /translation="MQINLDHNKKYLLACSYGPDSMALFSLLLKGNYNFAVAHINYGL
                     RGEESEKETNDLALYCKEHNIAFFVKWVNGKKIKGNFQNEARKVRYEFFEEIIKEHDF
                     DALLTAHHADDVIETYLMQQNSKRETFYYGIREEIVLNNFLVIRPLLNYYKNDLVFYC
                     VEHKVPFAVDSSNLELTYTRNKIRHNLVKKMSVEQKDKLLLDIKGRNKDLKAMTNKLE
                     LLLENSTISITEFIKLNNREQQHLLYMLFHNKGIADKFSKGRNNQIKDVVLSDKNSAM
                     IKVIDSYYLVKYEDKFSLININNYQPYKYIVETPETIKTPQFVAFFGKNNVFPNIPAS
                     EYPLTIATQGKNQKYKIKGYEKTINRLFIDMKMPRHYRLIWPVVTNKNGEVIYVPRYR
                     ANYIPKATDLFKIYIK"
     gene            3510..5495
                     /gene="ftsH"
                     /locus_tag="PLU08_03195"
     CDS             3510..5495
                     /gene="ftsH"
                     /locus_tag="PLU08_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOQ70732.1"
                     /translation="MSNPNPRPNPRSVPPRRSLRTLLPYLLLFAVIGVVIWLAFFTGN
                     AKTQALLQTELAAYLQTNAGVIDTITIVTTETTTKITGILKDGSKFTALVDRTFYENI
                     LQPTLFAADGTALVDFKMVIVNAYQRTFWNTIGPSLLYLVLFVVAMIFLYSRMAGAVS
                     SSNTKAMEFNRSRARLETKVKKTFDDVAGADAEKQEMMEIVDYLRNPKKYTKFGAKLP
                     KGVLLVGPPGTGKTLLAKAVAGEANVPFYSISGSDFVEMFVGVGAGRVRDMFQQAKKT
                     APCLIFIDELDAVGRQRGAGLGGGNDEREQTLNQLLIEMDGFSDNSGIIVLAATNRDD
                     VLDPALLRAGRFDRKITVSLPDRAGRTAIFGVHARGKIIDESIDFDAISRRTVGFSGA
                     DIESVLNDAAILAIRDNADKIMLQHIDEAIDRRIAGPARTSRQLNEHERKVIAYHEAG
                     HAVVGLNHPFSDKVQKITIIPRGQTGGHVLMTPEEDRFLMTKNQLIARIVGYLGGRTS
                     EEVFFDDVSTGAQNDIEVATRIARLMVTEFGMSDLGPIQYERDTGSVFLGRDYTSSQK
                     NFSQQVALEIDKEVRKIIEQAHDKAREIIEKHKDDVILIAETLLDRETITAEQIDRLL
                     KDRKLDEPKQAVIGEVVLTEKPKVEPTPTPVPEDDKK"
     gene            5534..6514
                     /gene="dusB"
                     /locus_tag="PLU08_03200"
     CDS             5534..6514
                     /gene="dusB"
                     /locus_tag="PLU08_03200"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOQ70733.1"
                     /translation="MWKIGNIEIKGKTILAPMAGITTFAYREFMRSFGVALCYTEMIS
                     DSGLIFNNEKTYEYLPRPEEKRPTSVQLFGGETTKLLVAIEKIEKHTDNYDFLDLNLG
                     CPVPKVTRNNGGSAWLKHPNELIAMVEKVVKRSTKPVTVKIRIGWDEKHINFKELLPK
                     LEEAGVSLIAIHLRTTKQLYGGKARYDLAQNIQNLVSIPLVVSGDIYTLDDAIKALEI
                     TGAQAVMLARGQLGNPFLATQIETYFKTGERLKDPSLYQQLDYLWTLANMMVEEKGEH
                     MGILVMRGIAHHFIKGFPFTKTYKVKLTKMSTLNDLELIIKEIKNQALLI"
     gene            6555..8039
                     /gene="lysS"
                     /locus_tag="PLU08_03205"
     CDS             6555..8039
                     /gene="lysS"
                     /locus_tag="PLU08_03205"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOQ70734.1"
                     /translation="MPREFSDQELVRRAKAEELSAQGIDPFGKKYERTDTSLSIKEKA
                     GEFTKEELEEKAIKVKTAGRIMLMRIMGKASFFTIQDQEGRIQAFISKQGVGEEVYDL
                     FKSADIGDIVGVEGTIMRTKTGEVTVKVEVYTHIVKALRPLPEKFHGLTDVEERYRRR
                     YVDLIMNESSKHVALTRPKIIRAIQKFLDDRGFVEVETSMLNSVLGGASAKPFITHHN
                     TLNRDFYLRIATELPLKRLLVGGIEKVYEIGRLFRNEGMSTRHNPEFTTVEAYQAYAD
                     LDDMYELCESLVRELAIKVTGDTKLPWGDEIIDVGAPFRKVSMVDLVKEKTGVDFRKI
                     KTFEEAKAIADKHEIHVEKHWTGSGHILNAFFEEFCEADLIQPTFVYGHPLEISPLAK
                     KAEDPRFTERFELFIKANELANAFTELNDPRDQYERFKAQLKARELGDEEASEMDEDF
                     VEALEYGMPPAGGIGIGIDRLVMFLLNQTSIREVILFPTMRDNK"
     gene            8188..8475
                     /gene="rpsF"
                     /locus_tag="PLU08_03210"
     CDS             8188..8475
                     /gene="rpsF"
                     /locus_tag="PLU08_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOQ70735.1"
                     /translation="MKKYEIMYILLADLSEEERKAEMESLHQMITNLGGKIVDVDTEN
                     WGLRDFAYPINDLLKGYYVVINVESDAAAIAEFERLSKLNKNVIRHLVIAK"
     gene            8494..8907
                     /gene="ssb"
                     /locus_tag="PLU08_03215"
     CDS             8494..8907
                     /gene="ssb"
                     /locus_tag="PLU08_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014645360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOQ70736.1"
                     /translation="MINRVVLVGRLTHDPELRKTSSGASVTNFSIAVNNRFARDQENS
                     TSFFRVSCWNQTADFVAQYARKGNLVGVDGRIEQRSYVNNAGQKVNTVDIIADSVQLL
                     ESKSESSEGTGYQSDAEPATDLGEGIDISDDDLPF"
     gene            8932..9159
                     /gene="rpsR"
                     /locus_tag="PLU08_03220"
     CDS             8932..9159
                     /gene="rpsR"
                     /locus_tag="PLU08_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOQ70737.1"
                     /translation="MSYRKSRPRRKVCYFKQNKITEIDYKNVELLKRFVTPNGKITPR
                     RVTGTSAKYQRQLAVAIKNARFMGLLPYYVS"
     gene            9214..10167
                     /locus_tag="PLU08_03225"
     CDS             9214..10167
                     /locus_tag="PLU08_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70738.1"
                     /translation="MKKTKFYYFELGLVILITLGLYVFSTNFLLLHYIGFLGPVPLAV
                     FIIRHKIKDGIFPAIFMILMGTLIGHFLPVGQGSFMRGFLFMIYAMTVGFLHGTISKT
                     KINPLIEILIIIGVEIFYGFLLVIVFYVQKDPHFAYDIQFGQYLELFIKLFKIDETSN
                     YAQNVGVIIKNGVIAYTIVFSFISVLLTHILIHLTRKFIYREKMEHGPLHGLEFTISR
                     VAAITYLVGCVVVLVLSFLLTKELNLLGISSINAIFTVFFSVTIIFVFQGILAVNLRL
                     EAKGCNNLSTLVFISGILLSPIFAIIGAINPLLPRKEKAVV"
     gene            10249..10845
                     /locus_tag="PLU08_03230"
     CDS             10249..10845
                     /locus_tag="PLU08_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70739.1"
                     /translation="MARKKKNDVALYDDSSYEEDDLKYAADEEVYAESLEDDEGKAKK
                     KRKKKKKEPKDPKERKDGSETRGGKTLTDRYHYRGRYYLLPERELRKQLRFYQKVFNY
                     RFLGIIAGVIALIFSILSLTFNAYFAVFTLLIVITGLIYGLLGMLKLRGLNWLFVLIG
                     IGLNIMAIVLAMKPLTFVFKHLGEIMALINEYMSSAGS"
     gene            10967..13054
                     /locus_tag="PLU08_03235"
     CDS             10967..13054
                     /locus_tag="PLU08_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOQ70740.1"
                     /translation="MIKAITKLRKRTIILVIVEGLLLGLISLFYYLNLFNFQEIFVPP
                     VIFSVAIGVVFFNLFVFIFITARINKLRKKNTFELVNLLGGDVKEAYNFGMLGLVVVD
                     NDFNVTWASELFKERRLLIIDKNILAWKPELIRLVELSEVLADNVEDTIKVEIESRNY
                     EVKYLSKAGIFLFKDITEFEYLFDSNQATAPVLGVVVIDNFVDIVVKGEHSNPIFGTV
                     NSLILEYFSNYGVLLKQYREDAYLLILNKENFDKLYADNFSILQKIKDKTSNQDYQIS
                     LSMAFAYNFPDVLKLNELVLNAIDTAMARGGDQVVIARYGEESEFIGGATSQAEKRNK
                     TAIRLYADTIFRHIREAKNVLIMTHTDTDFDALGASLGLKAMADYVKKTQKGTKVQFQ
                     PARIVYEHNLTELKTRMAVNRMFTKQEMNDMFISPEEIDDQKGTASLVNSGTLLIIAD
                     VSCPQLTLHPKLLERVDKVIVIDHHRRTDEFIDQRLYDYIETSASSTCEIVTELLHYG
                     NTPNFVLPPKYATFMLAGIYLDTNYFRTKTTGSRTFLASMVLEDWGADVMTADDFLKD
                     NVEEYALITKIMNSMTTLSVGIVFLRADETDIVTPETLSKVANQSMRIKGIQASFVIG
                     YTAPKVVKVSGRSNGEINVQFLLEKVGGGGHQASAAAVFTDKTLDDVQEILEDVINQN
                     LSQAKVVGGKKGG"
     gene            13056..13517
                     /gene="rplI"
                     /locus_tag="PLU08_03240"
     CDS             13056..13517
                     /gene="rplI"
                     /locus_tag="PLU08_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOQ70741.1"
                     /translation="MKVILLKDVKKVGKKDEIVDVSDGYATNFLIPRKLAVPYNERTI
                     SQLKANQARALAEEKAHKEEALVNKKKLEEKEISFILAIGEKGKAFGAITAKKVEEKI
                     KEEFKINVDRRKFVNFQPLNQLGPTIVEIELFKDVIAKVKVLVKDKEINYS"
BASE COUNT         4732 a         2136 c         2617 g         4065 t
ORIGIN      
        1 aaaattaaat attttggcaa tgaagaatta tcaattccaa tggaacaaat tcatttaatt
       61 cgcaaatata gtggccgaga aggcgttgca ccgcgactgc ataaaattgg ttcaccagta
      121 tggcagagaa caaaggttga aataagcaaa aaaattgaag acattgctga agaacttatt
      181 gctattcaag cagcgcgtta tcaaagacca ggttttgctt ttccgcctga tgacgaattt
      241 caaaaagaat ttgaacttgg tttcccatat gaattgacct acgaccaaga aatagcgctt
      301 cgggaaatta aaaacgatat ggaatcacca cttattatgg accgacttct ttcgggtgat
      361 gtaggctttg gtaaaacaga aatagcactt cgcgccgcct ttaaagcaat aagcgctggt
      421 aaacaagtaa tgtttttatg ccccacaacg ttacttgcaa gacagcacta tgaggtgtca
      481 ctagagagat ttgctaattt cggtgtaaaa atagcaatga tttctcgtct tgttactcaa
      541 aaagaaaagg atgaaatcgt aaaagacttt caagctggca atattcattt gcttattggt
      601 acacatgctt tgtttaatat agatgttgaa agcgttgatc ttggattatt aattgtcgat
      661 gaagaacaaa ggtttggcgt tgaacaaaaa gagattatta aaaaaatgaa aacaagagtc
      721 gatgttttaa cattgagcgc cactcctatt ccgcgtacat tacaaatgtc attagttggc
      781 atccgcggct taagtcaaat taatactgct ccccaagaaa gaatgccaat tcaaacttat
      841 gtcattaaaa gagatgatta tgtaatcaaa gaattagtgg agcgcgagtt agggcgtggc
      901 ggacaagtat tttatttata taatcgtatt gcaacgatat accatgtcgc aaataagtta
      961 agcaggctag tgccacaagc taaaattggc gttgtacatg ggaaaatgga tcgcgctgat
     1021 attgaagatg taatgacaag tttctataat aacgaaatta atgttttagt cgctacttct
     1081 attattgaaa acgggattga tgtgccaaat gcaaatgtaa tcatcgttga aaatgctgat
     1141 acttttggac tatcgcaact ttatcaaata aaaggaaggg ttggaagagg aaaccgcatt
     1201 gcatacgcat atttacttta tcaaggcaac aaaataatga atgatgatgc gaaaaaaaga
     1261 cttcaagcaa ttcaagagtt tacagattta ggcagtggct acaaaatagc acaacgcgat
     1321 ttaatgattc gtggtgccgg tgatatttta ggtgcggaac aatctgggtt tattgatagt
     1381 atcggtattg atatgtattt aaagatgctt gaagatgcaa taaatgagaa aaaatataaa
     1441 ctaacaagtg atccaataga aaaaacaatc aatattagcg gattagaagc ttatattcct
     1501 gatgattttg ctagtaagtc agaaaaattt gatatttatc tcgaaattaa aaaaagtaaa
     1561 aagatgaaag aactaaaaga ctttgaagaa aaagtaatcg atatgtatgg taaattacca
     1621 cgttcattag aaatcttatt tttaaaacgc caagttgaac tagtggtaaa tagtcgcgct
     1681 aactttattg aagacattaa agatgaagat gtatttattg atattattcc aggaagagca
     1741 tttttagata tccctggctc agtagtaatg ttaaataatg aaatagggac aacataccag
     1801 aacattcgtt ttataaccca agatgataaa attaagttaa ggataattaa aagaggagat
     1861 tggtttcacg catacgcgtc aattgttaaa gcaattgtca tgattgttcg tgaaacaatg
     1921 aaaaataaag atgagactag ataaatttct aaaattgacg cgattaatta aacgaagaac
     1981 ggtggcaaaa gatataagta cggccggaaa agttatcgtt gataaaagaa ctgttaaacc
     2041 atcatacact gttaaagttg atgatgtttt agaattatat ttaggagcac atatcgttgt
     2101 cgtaaaagta cttacggttg atgaaaaaga aataaaaagt aatccagaaa aaggatatga
     2161 attattaagt agaacaaaaa atgcaaatta atcttgatca taacaaaaaa tacttattag
     2221 cgtgcagcta tggtcctgat tctatggcgc ttttttcact attgctaaaa ggaaactata
     2281 actttgcagt tgcgcatatt aattacggat taagaggcga agagagtgaa aaagagacta
     2341 acgatctcgc actttattgc aaagaacata atattgcttt ttttgtaaag tgggttaacg
     2401 gtaaaaaaat caaaggtaac ttccaaaacg aagcaagaaa agttcgttat gagttttttg
     2461 aagagattat taaagaacac gattttgatg cactattaac agcgcatcac gctgatgacg
     2521 tgattgaaac atatttaatg cagcaaaata gtaaacgtga aacattttac tatggaatta
     2581 gagaagaaat tgttctaaac aactttcttg ttattcgtcc gttactaaac tattacaaaa
     2641 atgatctagt gttttattgt gtcgaacaca aagttccttt tgcagtagac agttcaaatt
     2701 tagagttaac atacaccaga aataaaattc gccacaactt agttaaaaaa atgtctgttg
     2761 aacaaaaaga caaactactc ctagacatta aagggcgcaa taaagattta aaagcaatga
     2821 caaataaact tgaattattg ttagaaaata gtactatttc cataacggaa tttattaaac
     2881 taaataatag ggagcaacag catcttttat atatgctttt tcataataaa ggaattgcag
     2941 ataagtttag taagggcaga aataaccaga ttaaagatgt tgtgttaagt gataaaaatt
     3001 cagcaatgat taaagtaatt gattcatatt atttagtgaa gtatgaagat aaattttcat
     3061 taattaatat caataattat cagccttaca aatatattgt tgaaacacca gaaacaatta
     3121 aaaccccgca attcgtggct ttttttggca aaaacaatgt atttcctaat attcctgcta
     3181 gcgaatatcc tttgacaatt gctacacaag gaaaaaatca aaagtataaa atcaaaggtt
     3241 atgaaaagac aataaatcgt ttatttattg atatgaagat gccgcgtcac tatcgactta
     3301 tttggccggt tgttacaaat aaaaatggtg aagtaattta tgtcccgcgc tatcgcgcta
     3361 actacattcc aaaagcaacg gatttattta aaatttatat taaataaaac gcaaatgttg
     3421 aaatctaaga ttatactctg tataataact ttcagtgttt atttatgtta cacatattcg
     3481 aaattaatct ataacccaag gaggtcttaa tgagcaatcc aaatccacgt ccaaacccta
     3541 gaagcgttcc accgcgtcgc tcgctgcgaa cccttttacc ttatttactt ttatttgctg
     3601 taatcggtgt tgttatctgg ttagcatttt ttacgggaaa cgctaaaact caagcactac
     3661 ttcaaaccga actagcagca tacttacaaa caaatgctgg tgttattgac acaattacta
     3721 ttgtaactac cgaaaccacc acgaaaatta ctggtatttt aaaagacggt agtaagttta
     3781 ccgctttagt agaccgaaca ttttatgaaa acatcttaca accaacatta ttcgccgctg
     3841 atggaacggc gcttgttgac tttaagatgg ttattgttaa tgcttatcaa cgcacatttt
     3901 ggaacactat tggaccaagc ttactttatc ttgtcctatt tgtagtggca atgatattcc
     3961 tttactcaag aatggctggt gcagttagca gttcaaatac taaagccatg gaatttaatc
     4021 gttcacgcgc tcgcttagaa acaaaagtta aaaagacgtt tgacgatgtt gcaggcgctg
     4081 atgctgaaaa acaagagatg atggaaattg ttgattattt acgtaatccc aaaaaatata
     4141 caaaattcgg tgcaaaacta ccaaaaggtg tacttcttgt ggggccgcca ggcaccggta
     4201 aaacactttt agctaaagca gtagcaggtg aggctaatgt gcctttctac tcaatttcag
     4261 gttctgactt tgttgaaatg tttgttggtg ttggtgcagg tcgcgttaga gacatgttcc
     4321 aacaagcaaa gaaaacagca ccttgcttaa ttttcattga tgaattagac gctgttggtc
     4381 gtcaacgtgg tgccggactt ggtggtggaa atgacgaacg cgaacaaacg cttaaccaac
     4441 ttttaattga gatggatggt tttagtgata actcaggaat tattgttctt gcggcgacaa
     4501 accgcgatga cgttttagac ccagccttat tacgtgcagg acggtttgac cgtaaaatta
     4561 ccgttagtct accggatcgc gctggtagaa cagcaatctt tggtgttcat gctcgcggta
     4621 aaattattga tgaatcaatt gactttgatg ccatttcacg ccggactgtt ggattttcag
     4681 gcgcagatat tgaaagcgtg ttaaatgatg cagcgatctt agcaattcgc gataatgctg
     4741 acaaaattat gttacagcat attgatgaag ctattgatcg tcgtattgct ggtcctgccc
     4801 gcacaagccg acaacttaat gaacacgaac gcaaagttat tgcttatcac gaagcgggtc
     4861 acgctgttgt ggggctaaat cacccattta gtgataaagt gcaaaaaatt acaattattc
     4921 cacgtggtca aactggcggt catgtactta tgactcctga agaagaccgc ttcttaatga
     4981 ctaaaaacca attaattgct cgtatcgtcg gttatttagg cggtcgtaca agtgaagaag
     5041 tattctttga tgatgtatca acaggcgcac aaaatgatat tgaagtcgct acaagaattg
     5101 ctcgactgat ggttactgaa tttggtatga gtgatttagg tccgattcaa tatgaaagag
     5161 atacaggcag tgtcttttta ggacgcgatt acacttcaag tcaaaagaac tttagccaac
     5221 aagttgcgct tgaaattgac aaagaagtac gcaagattat tgaacaagcc catgataaag
     5281 ctcgggaaat tattgaaaaa cataaagatg atgttattct tattgccgaa acattacttg
     5341 accgcgaaac aattacggca gaacaaattg atcgtttgtt aaaagatcgt aaactcgatg
     5401 aaccaaaaca agcagtgatt ggtgaagttg ttttaactga aaaaccaaaa gttgaaccaa
     5461 caccaacacc tgttccggaa gacgataaaa aataagaaat cgaaagccga agcaattcgg
     5521 ctttttaata acaatgtgga aaataggaaa catagaaatt aaaggtaaaa ccattcttgc
     5581 tccaatggcg ggcataacga cttttgctta tcgcgaattt atgcgctcct tcggtgtagc
     5641 gctttgttat accgaaatga taagtgatag cggccttatt tttaataatg aaaaaacata
     5701 tgaatattta ccacgaccag aagaaaaaag accaacatcg gtgcaacttt ttggcggtga
     5761 aacaactaag cttttagtgg caattgaaaa gattgaaaag cacacggata attatgattt
     5821 tcttgattta aatttaggat gtcccgtacc taaggtaaca cgaaataatg gcggttctgc
     5881 atggttaaag catccaaacg agcttattgc tatggttgaa aaagttgtta aaagaagcac
     5941 aaaaccagtg actgttaaaa ttagaattgg gtgggatgaa aagcatatta atttcaaaga
     6001 actattacca aaattagaag aagcaggcgt gagtttaatt gccattcact tgcgaacaac
     6061 aaaacaactt tatggcggaa aagctcgcta cgatttagcg caaaatattc aaaatttagt
     6121 aagtataccg ctcgttgtaa gcggggatat ttatacactt gatgatgcga taaaggcgct
     6181 tgaaataact ggcgcacaag cagtgatgct agcgcgcggt caacttggaa atccgttttt
     6241 agcaacacag attgagacat actttaaaac gggagagaga ttaaaagatc cctcactata
     6301 ccagcaatta gattatttgt ggacactggc aaatatgatg gtcgaagaaa aaggagaaca
     6361 catgggaatc ttagtcatgc gcggaatcgc tcatcacttt attaaagggt ttccgtttac
     6421 caaaacatac aaagttaaac taactaaaat gtcgacttta aacgacttag aattaataat
     6481 taaggagatt aagaaccagg ctttgcttat ataagcaaaa agtggtaaaa tgtccacatc
     6541 taaaggagtt atttatgcca agagaattta gtgatcaaga attagtgaga cgcgctaaag
     6601 cggaagaact tagtgcgcaa ggtatcgatc catttggaaa aaagtatgaa cgaacagata
     6661 ctagtctttc aattaaagaa aaagctggtg aatttactaa agaagaatta gaagaaaaag
     6721 ctattaaagt taaaacagcg ggacgcataa tgctaatgcg tattatgggt aaagctagtt
     6781 tttttacgat ccaagaccaa gaaggaagaa ttcaagcctt tattagtaaa caaggtgttg
     6841 gtgaagaagt ttacgactta tttaaaagtg ctgatattgg tgacatcgtt ggtgttgaag
     6901 gaacaattat gcgtaccaaa acgggcgaag tcacagtgaa ggttgaagtt tatacccata
     6961 ttgtaaaagc attacgacca cttccggaaa agtttcacgg attaactgat gttgaagagc
     7021 gttatcgtcg ccgttatgtt gatttaataa tgaatgaatc atcaaaacat gttgccttaa
     7081 ctcgtccgaa aattattcgt gcgattcaaa aattcttaga tgatcgtgga tttgttgaag
     7141 ttgaaacaag tatgttaaat agtgtacttg gtggcgcgag cgctaaacca tttattactc
     7201 atcataatac gcttaatcgt gacttttacc tgcgaattgc cacggaatta ccattaaaac
     7261 gcttattagt cggtggcatt gaaaaagtat atgaaattgg tcgtctcttc cgtaacgaag
     7321 ggatgagcac gcgtcataat ccagaattta caacggttga agcttatcaa gcttacgctg
     7381 atttagatga tatgtatgaa ctttgcgaaa gcctagtaag agaacttgcg attaaagtca
     7441 caggtgatac taaattacca tggggcgatg aaattattga tgttggtgcg ccgttccgca
     7501 aagtatcaat ggttgatttg gtcaaagaaa agacaggtgt tgactttaga aaaattaaaa
     7561 catttgaaga agcaaaagca attgcggata aacatgaaat tcatgttgaa aagcactgga
     7621 ctggctcggg tcatattctt aatgccttct ttgaagaatt ttgtgaagca gacttaattc
     7681 aaccaacatt tgtatatggg catccacttg aaatttcccc actagcgaaa aaagccgaag
     7741 atccgcgttt tacagaacgc tttgaattat ttattaaagc aaacgaacta gcgaacgcct
     7801 ttactgaact taatgatccg cgcgatcaat atgaacgctt taaagcacaa ttaaaagcgc
     7861 gagaactagg tgatgaagaa gctagtgaaa tggacgaaga cttcgttgaa gcattagaat
     7921 acgggatgcc accggcaggc gggattggaa tcggtattga tcgtcttgta atgtttttat
     7981 taaatcaaac atcaattcgg gaagttattc ttttcccaac aatgcgtgat aataaataaa
     8041 ataaaattgt aattattact agcgaacaag gtgtgccttt acaaacgcta gtaatagtaa
     8101 tataattaca acgctatatt actgcttgta atatactcca tgctgttggt aagcaacagc
     8161 cgaaatagac caaagggagg tgtgcttatg aaaaagtacg aaatcatgta tattcttctt
     8221 gcagacttaa gcgaagaaga acgtaaagcc gaaatggaaa gtctccatca aatgattacc
     8281 aatttaggcg gtaaaattgt tgatgtagat accgaaaatt ggggcttacg tgattttgcg
     8341 tatccgatta atgatttact taaaggatat tatgtagtca ttaatgtcga aagtgatgct
     8401 gcggcaatcg ctgaattcga acggttatca aaattaaata aaaatgtaat tcgtcatctt
     8461 gttatcgcta aataaaaacg aggaggaata acaatgatta atcgagtagt cttagtggga
     8521 agacttaccc acgacccaga attacgtaaa acatcgtcag gtgcatccgt taccaacttc
     8581 agcatcgcag taaacaatcg tttcgctcgc gaccaagaaa atagtacaag tttctttaga
     8641 gtctcatgct ggaaccaaac cgcagacttt gtcgctcaat acgcacgcaa aggaaactta
     8701 gtgggagttg atggacgcat tgaacaacgt tcttatgtaa ataatgctgg acaaaaagtt
     8761 aataccgtag acattattgc cgattcggtt caattacttg agtccaaatc tgaaagtagt
     8821 gaaggaaccg gttatcaaag tgatgctgaa cccgctactg acttaggaga aggaatcgac
     8881 atctctgatg atgatcttcc attctaaatt attaaaggag aatagtttat tatgtcatat
     8941 cgcaaaagta gaccacgacg taaagtttgc tatttcaaac aaaacaaaat tactgaaata
     9001 gattataaaa atgttgagct tttaaaacgt tttgttactc caaacggaaa aattactccg
     9061 cgtagagtta ctggaacatc tgcaaagtat caacgtcaac ttgcagtcgc aattaaaaac
     9121 gcacgcttta tgggattact tccatattac gtgagctaaa aaaagaatga agagagagcc
     9181 tcacgacagt gaggttcttt attttagtta attatgaaga aaacaaaatt ttattatttt
     9241 gaattaggct tagtaatatt aattacatta gggctttatg ttttttccac taatttttta
     9301 ttactacatt atattggatt tctaggtcca gtaccgcttg cggtttttat tattcgtcat
     9361 aaaattaaag acggaatatt tcccgccatt tttatgatat taatgggaac attaattgga
     9421 cactttttac ctgtgggcca aggtagtttt atgcgtggtt tcttatttat gatttacgca
     9481 atgacagtag gatttttaca cggcacgatt agcaaaacga aaattaatcc cttgatcgaa
     9541 atattaatta tcattggcgt agaaatattt tatggctttt tattagtcat cgttttctat
     9601 gttcaaaaag atccgcactt tgcttatgat atccaatttg gtcagtatct agaattattt
     9661 attaaactat ttaagataga tgaaacatca aattacgcgc aaaatgtcgg tgttattatt
     9721 aaaaatggtg ttattgctta cacaattgta tttagtttta taagtgtttt attaacgcat
     9781 attctcattc acttaacacg aaaatttatc tacagagaaa aaatggaaca cggcccatta
     9841 cacggtttag agtttactat ttcgcgtgtc gcggcaataa cttatttagt tggttgtgta
     9901 gtagttttag tactatcttt ccttttaaca aaggaattaa atctgctagg aatttctagt
     9961 atcaacgcga tctttactgt tttcttttct gtcacaatta tttttgtgtt ccaaggtatt
    10021 ttagcggtta atcttcgcct tgaggctaag ggttgcaata atcttagtac gctagtattc
    10081 atcagtggta tattactttc tcccatcttt gcaataattg gagcgattaa tccgctctta
    10141 ccccgcaaag aaaaagcggt tgtataatca ttaaaataaa atcgctaata aacaaattgt
    10201 ttattcataa tcaaatttat gctaaattgt atatatagga attaaattat ggccagaaag
    10261 aaaaagaatg atgttgcttt atatgatgac tcttcatacg aagaagatga tttaaagtac
    10321 gctgctgatg aagaagttta tgctgaatca ttagaagatg atgaaggcaa agcaaaaaag
    10381 aaacgcaaga aaaagaaaaa agaaccaaaa gaccccaaag agcggaagga cggaagcgaa
    10441 acgcgtggcg gtaaaacttt aacagaccgc tatcattaca gaggtcgata ttatctttta
    10501 cctgaacgcg aattacgtaa acaactgcgc ttctatcaaa aagtatttaa ttatcggttt
    10561 ttaggaatca ttgcaggtgt gattgcctta atcttttcta ttctttcgct gacatttaat
    10621 gcttattttg cagtctttac actacttatt gttatcacgg gactaatcta tggtctttta
    10681 ggaatgctta agttacgtgg cctaaattgg ctttttgtct taattggaat tggtttaaat
    10741 atcatggcga ttgtattagc gatgaaacca ttaacatttg tgtttaaaca tcttggtgaa
    10801 attatggcgc tcattaatga atacatgagt tctgccgggt cttaattaca ctagttatca
    10861 acattaaagt aataattttc aacaaaatat atattatggg acatgaaagg tattaaagat
    10921 acctttttta ttgtataatt actctaaaga aaaacgaggt attgctatga tcaaagctat
    10981 tactaaatta cgcaaacgaa cgattatcct tgttatagtt gaaggacttt tactaggttt
    11041 aattagtctc ttctattatt taaacttatt taattttcaa gaaatctttg taccgccagt
    11101 tattttttct gtcgcgatag gtgtcgtttt ctttaatttg ttcgttttta ttttcattac
    11161 cgcacgaata aataaattac ggaaaaagaa tacatttgaa cttgtaaact tattaggtgg
    11221 cgatgttaaa gaggcttaca actttggaat gctaggtctt gttgtcgtcg ataatgattt
    11281 taatgtcaca tgggccagtg aattatttaa agaacgtcgt cttttaatta ttgataaaaa
    11341 cattcttgct tggaagcctg aattaattcg gcttgttgaa ctaagtgaag tgcttgcaga
    11401 taatgttgaa gacacaatta aagttgaaat cgaatcacgg aactacgaag ttaaatattt
    11461 aagcaaggct ggtattttct tgtttaaaga tattactgaa tttgaatatc tctttgatag
    11521 taatcaagca acagcacctg ttttaggcgt agtggttatt gataactttg tggatattgt
    11581 tgttaaaggt gaacactcta atccaatttt tgggacagtt aacagtttaa ttctcgaata
    11641 cttttcaaat tatggggtgc tgcttaaaca atatcgtgaa gatgcttatc tacttatttt
    11701 aaacaaagag aactttgata agttatacgc tgataatttt tcaattttac aaaaaatcaa
    11761 agataaaaca tcgaatcaag attatcaaat ttcgttatca atggcgtttg cgtataattt
    11821 ccctgatgtt ttaaaactaa atgaactcgt ccttaatgcg attgatacag caatggcgcg
    11881 tggtggtgac caagttgtta ttgcccgtta cggtgaagaa agtgaattta tcggtggcgc
    11941 aacttcacaa gctgaaaaac gtaataaaac agcgattagg ttatatgcgg atacaatttt
    12001 ccggcatata agagaagcaa aaaatgtttt aatcatgaca cacaccgata ctgactttga
    12061 cgctttgggt gcgtcgcttg gtttaaaagc tatggccgac tatgtaaaga aaacacaaaa
    12121 aggcacaaag gttcaatttc aacctgcacg catagtttat gaacacaatt taactgaatt
    12181 aaaaacaaga atggctgtaa acagaatgtt tacgaaacaa gaaatgaatg atatgtttat
    12241 ttcaccagaa gaaattgacg atcaaaaagg aaccgcgagt ctagttaatt ccggcacact
    12301 tttaattatt gctgatgttt catgtccaca attaacgctt catccaaaac tccttgaacg
    12361 cgtggataag gtaattgtga ttgaccatca tcgtcgcaca gacgaattta ttgatcaaag
    12421 actttatgac tatattgaaa caagcgcaag ttcaacttgt gaaattgtga ctgaactact
    12481 acattacggt aatactccta acttcgtatt accaccaaaa tatgcgacct ttatgcttgc
    12541 tggaatttat ttagacacaa actactttag aacaaaaaca acaggatcac gtactttctt
    12601 agcaagtatg gtgttagaag attggggcgc tgatgttatg acagcggacg atttcttaaa
    12661 ggataatgtt gaagaatatg cacttattac caaaattatg aatagcatga caacacttag
    12721 tgttggtatt gtcttcttaa gagcagatga aacagacatt gttaccccag aaactttatc
    12781 gaaagttgca aaccaaagca tgcgcattaa aggtatacaa gcttcgtttg ttattggtta
    12841 cacggcgcct aaggtcgtta aagtcagcgg tagaagtaac ggtgaaatta atgttcaatt
    12901 cttacttgag aaagttggag gcggtggtca ccaagcaagc gctgcagctg tttttacgga
    12961 taagacatta gatgatgtcc aagaaatttt agaagatgta attaatcaaa atctaagtca
    13021 agccaaagtc gttggcggca agaagggagg ataaaatgaa agtaatttta ttaaaagacg
    13081 ttaaaaaggt cggaaaaaag gatgaaattg ttgatgttag tgatggctac gccacaaact
    13141 ttttaatccc acgcaaactt gctgttccgt ataatgaacg gactatttcc caattaaaag
    13201 caaatcaagc gcgtgcttta gccgaagaaa aagcacataa agaagaagcg cttgtcaaca
    13261 agaaaaaact agaagaaaaa gaaattagtt ttattctcgc tattggtgaa aaaggtaaag
    13321 cttttggggc gattaccgct aaaaaagttg aagaaaagat taaagaagaa tttaaaatta
    13381 atgttgatcg ccgtaagttt gtgaactttc agccactaaa tcaattagga cccacaatag
    13441 tggaaattga actttttaaa gatgtcattg ctaaagtaaa agttctagtc aaagataagg
    13501 agattaacta ttcttaatca agttaaatag taaatatata tcaaaaagat
//
LOCUS       DAOSTA010000023        13324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_4713_length_13324_cov_8.522873, whole genome shotgun sequence.
ACCESSION   DAOSTA010000023 DAOSTA010000000
VERSION     DAOSTA010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 13324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13324
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4713_length_13324_cov_8.522873"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1279)
                     /locus_tag="PLU08_03245"
     CDS             complement(71..1279)
                     /locus_tag="PLU08_03245"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOQ70742.1"
                     /translation="MKNKKFKRVFVVVMDSVGVGAMPDAHLYGDAGSHTLKHTAESKK
                     NGLHVPNLEKYGLGNLDDIKGVKKVKNHPNSYVMKMLEASTGKDTMTGHWEFMGIHTE
                     KPFKTFTDTGFPQALIDELSAKTGYKIIGNYSASGTEIIKELGEEQMRDNSLIVYTSA
                     DSVLQIAAHEEVTGLDELYRCCEIAREICLRPEYLLGRIIARPYIGSDPSNFKRTANR
                     HDYALSPPEKTALNILDDNGITVSAIGKINDIYNGFGITQAQKTISNEDGMDKTIAQA
                     KANEFNGLIFTNLVEFDSEYGHRRNPVGYADAIERFDKRLGELMEVVTEDDLVLLTAD
                     HGNDPTWHGTDHTREMVPLIAISKAFTKGRVLEHRKSFADIGATILANYNLKLTPTLI
                     GEVIDELLTE"
     gene            complement(1339..2121)
                     /locus_tag="PLU08_03250"
     CDS             complement(1339..2121)
                     /locus_tag="PLU08_03250"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOQ70743.1"
                     /translation="MKRRGFLISLITFMMISLTSCFKLAVQHKIHKFGYYEYIITGVH
                     KRLPRGGPAVVRIIGLTQSGLQQSVLDIPRTINDLPVVALGYGPMKSGGFPSPPYTFS
                     SKNLRKLYIHDNLVNIERGAFSTPENQNEFVPFNIMICAYRFEQIGEFNLNQLNKRFI
                     YRYIFENRDIDSNIKWGNSAIPANVEFLNNYSDEVNGGYYRLDNIEDGELIPIPPDPF
                     REGYIFDGWYSESECTNKFDFDIAPTIAENTELKIYAKWISA"
     gene            complement(2638..3510)
                     /locus_tag="PLU08_03255"
     CDS             complement(2638..3510)
                     /locus_tag="PLU08_03255"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOQ70744.1"
                     /translation="MHIDEAVRRYTNFLLLEKNLANTTVSAYLDDINLFHTTFNFTVT
                     EEISPMHVSDFINLESQKGLRASTIMRRLSSIYNYLLFLKDEGDYKYELKRIPGPAVT
                     KTLPTYLTNDEVDRLLSMPNLNTHRGVRDKAMLEVMYASGLRVSELLSLKREEVNFNR
                     GIIKIRGKGGKERIIPIGEFALEYLADYINNVRFHYEKSDKRTIFLNTNGKPLSRQYF
                     FKMIKAYAQEAEITANVSPHTLRHAFATHLLENGADLRAVQAMLGHTNIATTQIYTHV
                     STKRIISAYDKIMK"
     gene            complement(3513..4682)
                     /locus_tag="PLU08_03260"
     CDS             complement(3513..4682)
                     /locus_tag="PLU08_03260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOQ70745.1"
                     /translation="MSKVILHIDLNAFFAACEVLRDPTLKGKPLIVGGANRRGIVSTA
                     SYEARKYGIHSAMPTYKALRLCPNLIIKEPDFKLYHHYSSLFFNYLKDHVSPLLEVAS
                     IDECYIDATERLKNNRNPIGFIKELQQTLLQEIGLGCSIGVAPTKFLAKMASNYKKPL
                     GITIYRRRELANTLWKLPINEMYGIGKATAPRLLALDIKTIGDLATTEDPEVKKLLGK
                     SFFTFKEWANGRGSDEVIAEEEDPKSIGHSSTFMFDTENYEEISALFKEMSNSVSERA
                     KREGKVGLTIQIVMRYYNFENVNRSVTLNKPTNNAITIFYEAMKLFDKNYKDEPLRLV
                     GVTLQNLSDEGSLVEQLSLFDDFNEKSKTEVIIELLNEEYDKPPFLRLSDLKRDD"
     gene            complement(4841..5032)
                     /gene="xseB"
                     /locus_tag="PLU08_03265"
     CDS             complement(4841..5032)
                     /gene="xseB"
                     /locus_tag="PLU08_03265"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOQ70746.1"
                     /translation="MSKEKLSFEQKLARLNAIVSELESGKLSLDASLKLFEEGNALSK
                     ELATELNEAKLKIEELQGK"
     gene            complement(5025..6281)
                     /gene="xseA"
                     /locus_tag="PLU08_03270"
     CDS             complement(5025..6281)
                     /gene="xseA"
                     /locus_tag="PLU08_03270"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOQ70747.1"
                     /translation="MRSILSISELNAYVKNVIQRDAFLSYVEVRGEIINLKRYNTAIY
                     FTLKEEDKARLSVVSFNTKAFPTTLKDGDEVIATGRITVYEKSGNYQLVATEVSYYGL
                     GAKLLELAKLKVKLDKMGLFDQEKKRPLPKFPKVIGIITPKDSAAQSDLVTNINRRYP
                     LVTLKIVNATFQGESAPSSLISAFEVLQNEHIDVLIIARGGGSSDDLWAFNDESLIMA
                     LSAKKVPLISAIGHEIDTTLVDYLADVRASTPTAAAELVVPDQNELRQTLTDYSVRLE
                     NAMSTVLIQREKELNHLIIRPVLVNFGAIVENLTTKLLTLSTKLGARSAENIARAEII
                     LHSLKERLNKAITRTYNVINETLVAKSTRLEALNPTNVLNRGYAFISKNEEIIKSAKK
                     LKVKDEVKITFSDGSVLAEVKGGEDE"
     gene            complement(6281..6691)
                     /locus_tag="PLU08_03275"
     CDS             complement(6281..6691)
                     /locus_tag="PLU08_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOQ70748.1"
                     /translation="MISRNKSHEYGVQSVYAMLVFIKEDIAFDLPAVVSGVTGAEYSE
                     LPVFLKETLINIVKNYSEIVTNLTPYLRDWKFERLNLVTQAILIYSYAVYFYTEDKGK
                     SKAVIINVAIKLSKLFNPTEDYKFINAVLDGVLI"
     gene            complement(6675..7157)
                     /locus_tag="PLU08_03280"
     CDS             complement(6675..7157)
                     /locus_tag="PLU08_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70749.1"
                     /translation="MKEKSRINPKKIVFKLINFFLLLIIFSNLIGNFFGISSDYTYYH
                     WGLNSIAIFSRYIENSVLEIGMRIFLLGLTTMVSSALLFLLYHFARKGKIVPIVLAII
                     IYVIDYSLVFSPWYRESTFSYIWSSFIHLIMIALFMAAGLFLLLLNPGGNKEYYDFTE
                     "
     gene            complement(7169..7492)
                     /locus_tag="PLU08_03285"
     CDS             complement(7169..7492)
                     /locus_tag="PLU08_03285"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOQ70750.1"
                     /translation="MAEYVYIQKYNKQGIMGISYQVFDKIAEIATNEVSGATVSRKRY
                     SLSKPVRCTIRSGTVNVKISVKVSPNTNAEEVANEVRENVALSLTQMTELIPFDIDVE
                     VIDVG"
     gene            complement(7709..9955)
                     /gene="tkt"
                     /locus_tag="PLU08_03290"
     CDS             complement(7709..9955)
                     /gene="tkt"
                     /locus_tag="PLU08_03290"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOQ70751.1"
                     /translation="MCLRKRNTYDALSVAAIRATVIDITNTANSGHPGMAIGSAPLVY
                     NLYKNHLKIDPRNPEWIARDRFVLSAGHASALLYTVLHLAGYAIEIDDLKAFRQLNSI
                     TPGHPEYKMTPGVDATTGPLGQGLANAVGMALAEASLTARNPKYAHLLSHYTYCLVGD
                     GCLQEGISQEAVSFAGHQKLNKLIVFYDANDVTLDGPLSDSFSENVEARFKASGWNVL
                     KVDNGNNLKQIDKAIKKAKRSDKPTLVIMKTIIGQGSRQQGTHKVHGSPLGELDGTFA
                     KGTYHYPNAPFEIPLPVYESMRSQIARNGQKAYDTWAKSYTLFEADSDVEAEDLKIAL
                     NDDTESSLAMMKLTFEDELSEATRNTSGNVLAAVNRHLPHIMGGSADVASSVMTLING
                     ETNFTAKNRRGRNINFGIREFAMGAISNGMLLHGGLRTYVGTFLVFSDYLKNSMRLAA
                     LSHLPNIYLFSHDSVNLGEDGPTHQPVEHLAMMRAMPNFDLWRPGDARETFVAWKVAL
                     ESKTRPHALILTRQKLPLMAGSDKEKLIRRGAYVVSPENKRKKLDFIIIATGSEVNMA
                     LKAQNTLLIHGYNVRVVSMPSMEVFRRQDDAYKAEVLPLGKDKRIAVEILSSFGWHEW
                     ADHTMCLDRFGFSAPQKDILEHLKYTPEMLARLVEGIIDPKTLKKGEELYSGEAPNPI
                     PPVLSDLDTPAEGEELLTLEDVELKVSEKVEPEVTASETKEKQEATEAKAEVKTEAKP
                     KTKKTTKK"
     gene            complement(9959..11698)
                     /gene="aspS"
                     /locus_tag="PLU08_03295"
     CDS             complement(9959..11698)
                     /gene="aspS"
                     /locus_tag="PLU08_03295"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOQ70752.1"
                     /translation="MYRTIENGNINEKLVGQTVKIIGWVAKKRDLGGLTFVDLRDRSG
                     IVQVLINEGVARPDIRNEYIIQIEGVVTLKEVPNKNLKTGKVEVVASKVTLINKAKNP
                     PLIIADETDALEDVRLKYRYLDLRRKVLQDKLIARAKITKVTREFLDNEGFIEIETPI
                     LTKSTPGGARDYLIPSRLNPGTFYALAQSPQIYKQLLMIGGLEKYYQIARCFRDEDLR
                     SDRQPDFTQIDIETSFFTYEEVLDLNERLLQKIFKEVKGYDLKLPLPRLTYHEAVENY
                     GSDKPDLRFDLKIRDLSKVLSNEEYFKAKEDVRGLIINDPTNILSRKTLDELNLIVKQ
                     HGVNFLGVLKYDGTAFSGSLAKNITSSHTEVAEALGLAANDVVLLGFDAPLARILSAL
                     GAVRKELGVRFNLIDENAYSCLWVDSFPLFSYDPETRRFASEHHPFTQPKEEDIAKLT
                     TAPEEVLSYAYDIVINGYEAGGGSMRIYDQDLQNRIFEILGLSEKEIEYLFGFFIEAL
                     KYGTPPHGGIAFGLDRLTMILTGTDDIRDVIAFPKNLKGASLMSGEPSKVSNLQLDEL
                     YIKINTGKDGGEE"
     gene            complement(11703..13265)
                     /gene="hisS"
                     /locus_tag="PLU08_03300"
     CDS             complement(11703..13265)
                     /gene="hisS"
                     /locus_tag="PLU08_03300"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOQ70753.1"
                     /translation="MAIITSPKGTHDLIGNEDRAYAYISDTCGIVARLYGFDPLTTPI
                     FEANELFARGVGDATDIVRKEMYVFTDKGNRLMALRPEGTAGVMRSIIANKLYATNDL
                     PLRYYYSGPMFRYERPQLGRYRQFHQFGVESVGSTSIIADLEVILLAIHSLQALGLEN
                     ITTKINSLGKQEARDAYREALKKYYEPHLKDVCPDCKVRYEQNPLRMLDCKVPSDVEL
                     AKAAPKVTDFLTEEDKKDLEFIKTSLEEYGVNVEVDYGLVRGLDYYSGIIFEFVYIPP
                     SGKDYGAIGGGGRYDKLVEELGGPSLEGVGFAFGLERLYHILNEEGKLEHTAVKDDFI
                     FVAIGEEARTGGYSLLTALRANGLRGIMNFEDKSFKSLFKIAENRGARVALILGEAEI
                     KNEVVSVRNLETQEQVEVPFAEVINYILDLCEEYHACNCGCDHEDCDCDNHDHKCKCG
                     CEHEDCDCDHEECECDSHKENGECCGEHHHDEKHECCRKHEHEKNHECTGKGKGRGVN
                     CKKHEEKEEEKA"
BASE COUNT         3909 a         2731 c         2239 g         4445 t
ORIGIN      
        1 atcatgtggc cacatgattt tattgtgtgt ctacatttag ggtatcagta caaagtgagg
       61 ataatatgag ttattcggtt agtaattcgt cgattacttc accaattaag gttggggtta
      121 actttaaatt gtaattagcg aggattgtcg caccaatgtc ggcaaagctt ttacggtgtt
      181 caagcacacg ccctttagta aatgctttgc taatagcaat aagcggtacc atttcacggg
      241 tatggtcagt gccgtgccat gttggatcgt taccgtggtc agcagttaaa agcactaaat
      301 cgtcttcagt aacgacttcc attaattcac cgagacgttt atcaaagcgt tcaatggcat
      361 ctgcatagcc tactggattg cgtctatgac cgtattcact atcaaattca acgagattag
      421 tgaaaattaa accgttaaat tcattagcct tcgcttgggc aatcgtttta tccattccgt
      481 cttcattact tatagtcttt tgtgcttgtg taatgccaaa accgttataa atatcattaa
      541 ttttaccgat tgcactgacg gttatgccgt tatcatcaag aatattaagt gccgtttttt
      601 ccggtggaga aagtgcataa tcatgccgat tagcggtccg tttaaagttg cttgggtcac
      661 taccgatata aggacgagca ataattctac caagaaggta ttcaggccgt aaacaaattt
      721 cacgggcaat ttcacaacag cgatataatt cgtcgagtcc tgttacttct tcatgcgctg
      781 ctatttgtaa aacactatcc gcggatgtat aaacaattaa gctattatca cgcatttgtt
      841 cttcaccgag ttctttaata atctccgtac cacttgctga ataattacca atgatcttat
      901 aacctgtttt cgcacttaat tcatcaatta gtgcttgcgg gaaacctgta tcggtaaatg
      961 ttttaaacgg tttttccgta tgaatcccca taaattccca gtggccagtc attgtatctt
     1021 taccagtgct agcttctaac attttcatga catatgaatt agggtggttt ttaacctttt
     1081 taacaccttt aatatcatca agattaccta agccatattt ttctaaatta ggaacatgta
     1141 atccattctt tttactttcc gctgtatgtt ttaaggtatg actaccagcg tcaccatata
     1201 aatgtgcatc aggcatagca ccaacaccga cactatccat aacgacaaca aagacgcgtt
     1261 taaacttctt atttttcata atttaatcct ttcgatttca ggtttattat aacaaataat
     1321 aaaacattat atatatggtt aagcactaat ccatttagcg tatattttaa gttcagtgtt
     1381 ttcggcaatt gtgggcgcta tgtcaaaatc aaatttattg gtacattcac tttccgaata
     1441 ccaaccatca aatatataac cttctcgaaa aggatcaggt gggattggaa tgagttcacc
     1501 atcttcaata ttatcaaggc gataataacc accatttact tcatcagaat aattattgag
     1561 aaattcaaca ttcgccggaa tcgcactatt cccccatttt atgtttgaat caatatcacg
     1621 gttttcaaaa atgtatcgat atatgaatct tttattgagt tgatttaagt taaattcacc
     1681 gatttgttca aaacgataag cacatatcat aatattaaat ggtacaaatt cattttgatt
     1741 ttctggagtc gaaaacgcac ctcgttcgat atttacaaga ttatcgtgaa tatacaattt
     1801 tcgcagattt ttgctactga atgtataagg tgggctagga aaaccccctg atttcattgg
     1861 accatagcca agtgctacca ctggtaaatc atttatagta cgcggtatat ctagcacact
     1921 ttgttgcaaa ccactttgtg ttaacccgat aattctaact accgcaggtc ctcctcttgg
     1981 taaacgctta tggactcctg taattatata ttcatagtag ccaaatttat gaatcttgtg
     2041 ttgaaccgca agtttaaaac atgatgttaa tgaaatcatc ataaatgtaa ttaatgatat
     2101 taagaatccc cttcttttca tctaatgaaa ctcctttttt gaatgtctat aataatcttc
     2161 aataaatcca agcgttccat tttggaaagc ttctacatca tcaccaacaa gaacaagaat
     2221 accattaggc agacaataac taccattttc tgtacaaacc gtgatgttat cattattttt
     2281 cgccccgaaa acgaaaccca aattagcagg accaccacat actaaacatt gataatgtcg
     2341 tccaggtaat agatagttat taccatcaat tacatgacca aacgttccgt cttgtccaca
     2401 ttcacaagct gcatagtgtt cgcgctaggt tcgctaaagt gtcaactttg tcataggctt
     2461 ctttgccaat ggctcttttc tcttctttac tgtacatgtt ttcctcctga atgtatttta
     2521 gcaaaatcat gtggccacat gattttattg tgtgtctaca tttagggtat cagtacattt
     2581 cgatttcttt tttattataa caaataatag tttgtgcttt attaatttaa caccttatta
     2641 tttcataatc ttgtcatatg cactgatgat ccgtttagtg gacacatgtg tatatatttg
     2701 cgttgtagca atattagtgt ggccgagcat tgcttgtacg gctcttaaat cggcgccatt
     2761 ttcgagtaag tgtgtggcaa aagcgtggcg taatgtatgc ggtgatacat tagcggttat
     2821 ttcagcttct tgcgcataag ctttaatcat tttaaagaaa tattgacgac ttaatggttt
     2881 accattagtg tttaagaaaa tagtgcgttt atcacttttt tcataatgaa agcgaacatt
     2941 atttatataa tccgctaagt attcgagggc gaattcacca attgggataa tccgctcttt
     3001 cccacctttc ccgcgaattt taatgatgcc acgattaaaa tttacttctt cacgctttaa
     3061 acttaaaagc tcgctaactc gtaatccgct agcatacatt acttcaagca tcgctttatc
     3121 gcgaacaccg cgatgagtgt ttaagtttgg catacttaaa agtcgatcaa cttcatcatt
     3181 agtgagatat gttggcaatg ttttagtgac tgctggtcca ggaatgcgtt taagttcata
     3241 tttataatca ccttcatctt ttaaaaagag caaataatta taaatgcttg atagtctccg
     3301 cattattgtt gatgccctaa gtcctttttg actttctaaa ttaataaagt cactaacatg
     3361 cattggcgaa atttcttcgg taactgtgaa gttaaaggtc gtatgaaaga gattaatatc
     3421 atctaaataa gcacttaccg tcgtattagc aaggtttttc tcaagtaata agaaattagt
     3481 gtagcgtctc actgcttcat caatatgcat ctttaatcat ccctttttaa gtcacttagc
     3541 cgtaaaaacg gtggtttatc gtattcttca tttaataatt caataataac ttctgttttt
     3601 gatttctcat taaagtcgtc aaataatgaa agttgctcga caagactccc ttcgtcacta
     3661 agattttgta gtgttacccc tacaagccgg agcggttcat ctttataatt tttatcaaaa
     3721 agcttcatcg cttcataaaa tatcgtaatg gcattatttg tgggtttatt aagcgtgaca
     3781 ctgcgattta cattttcaaa attataatag cgcatgacaa tttgaatcgt taagccgacc
     3841 tttccttcac gctttgctct ttcactaaca ctattagaca tttctttaaa taaagcactt
     3901 atctcttcat aattttcagt gtcaaacata aaggttgatg agtgaccaat actttttgga
     3961 tcttcctctt cggcaattac ttcgtcactc cctctgccgt ttgcccactc tttgaaggta
     4021 aaaaagcttt taccaagcaa ctttttcact tccgggtctt cagttgtagc aaggtctcca
     4081 attgttttaa tatcgagcgc taaaagacgg ggtgcggtcg ctttaccaat cccgtacatt
     4141 tcattaattg gtaatttcca taaagtgttc gctaattcac ggcgtcgata aatagtgata
     4201 ccaagtggtt ttttatagtt acttgccatc tttgctaaaa atttagtggg agcaacaccg
     4261 atcgagcagc caagtcctat ttcttgtagt aaagtttgtt gtaactcttt aataaaaccg
     4321 attggattac ggttattttt aagccgttcg gtcgcatcga tatagcattc atcaatacta
     4381 gcaacttcaa gaaggggact aacatgatct tttaaataat taaaaaagag cgaagaataa
     4441 tgatgataaa gcttaaaatc aggttcttta ataattaaat ttggacacaa gcgcagcgct
     4501 ttatatgtcg gcatggccga atgaataccg tatttccgag cttcgtaact cgccgtgcta
     4561 acgattccgc gcctgttagc cccaccaaca attaaaggtt taccctttag ggtagggtca
     4621 cgcagtactt cgcacgcggc aaagaaagcg ttaaggtcaa tgtgtaagat aactttactc
     4681 ataaatatat tataactcac tattttggtt tacataataa ttatgtaaat aataagttcc
     4741 taataaaaaa taggaacgaa tgtcctattt tataacacta taaagcaact aaaagactac
     4801 taactaatta ctaataacat actaaagggt cgttttagcg ttattttcct tgtaattctt
     4861 caattttcag ttttgcttca tttaattctg tcgctagttc tttacttaaa gcgttgcctt
     4921 cttcaaagag ttttaaacta gcgtctaaac taagtttccc gctttctagt tcactaacaa
     4981 tagcatttaa gcgtgcgagt ttttgttcaa atgataattt ttctttactc atcttctcct
     5041 cctttaactt cggctagtac actgccgtca ctaaatgtaa tcttaacttc gtcttttact
     5101 tttaacttct tcgctgattt aataatttct tcattcttac taataaaagc atagcctcta
     5161 tttaatacat tcgtcggatt aagtgcttca agtcgtgtac tcttagcgac aagcgtttca
     5221 ttaataacat tatacgtacg agttatcgcc ttatttaagc gttcttttaa tgaatgcaag
     5281 ataatttccg ctcgagcgat gttttcggcg ctacgggctc ctaatttagt gctcagtgtc
     5341 aaaagctttg tcgttaagtt ttccacaatt gcgccaaagt ttacaagtac tggtcttatt
     5401 attaagtgat tcagttcttt ttcgcgttgt atgagcacag tactcatagc gttttcaaga
     5461 cgaacactat aatcagtaag cgtttgccgt aattcatttt gatcaggaac aacaagttcg
     5521 gcggcagcag ttggggtact agcgcgcaca tcggcaagat aatcgactaa tgttgtatcg
     5581 atttcatgtc ctatcgcact aataagtggg actttctttg cacttaaggc cataatgaga
     5641 ctttcatcat taaatgccca taagtcgtca ctacttccac cgccacgggc aatgattaag
     5701 acatcaatat gttcattttg taaaacttca aaggcactaa tgagtgaaga aggcgcactt
     5761 tccccttgaa aagtagcgtt aacaatcttt aaagtcacaa gcggatagcg acggttaata
     5821 ttcgtcacta aatcactctg tgccgcgcta tcttttgggg taataatacc aataactttg
     5881 ggaaatttag gtaatgggcg cttcttttct tgatcaaaaa gccccatctt gtctaacttt
     5941 actttaagtt tagcaagttc aagcaacttc gcaccaagtc cgtaataact aacttcagtg
     6001 gccacgagtt ggtagttacc acttttttca taaaccgtaa tacgacctgt ggcaattact
     6061 tcgtcaccat cttttagcgt cgtaggaaac gctttagtgt taaaacttac aacactaagg
     6121 cgtgccttat cttcttcttt taaggtgaag taaatcgcag tattatagcg ttttaagtta
     6181 ataatttctc cgcgtacttc aacataactt aaaaatgcgt cacgctgaat aacattttta
     6241 acgtatgcat taagttcact aattgataaa atacttcgca ttaaattaaa accccatcaa
     6301 gcacagcatt aataaattta taatcttcgg tcggattaaa aagttttgat aatttaatag
     6361 cgacattaat aatcactgct ttacttttac ctttatcttc agtataaaaa taaacagcgt
     6421 aactataaat taagatggct tgggttacta aatttaaacg ttcaaacttc cagtcgcgta
     6481 aataaggtgt taagttcgtc acaatttcac tatagttctt aacgatgtta attaatgttt
     6541 ctttaagaaa tacggggagt tcactatatt ctgcaccagt tacaccgctt acaaccgcag
     6601 gtaaatcaaa agcaatgtct tctttaatga aaacgagcat tgcatagaca ctttgcacac
     6661 catactcatg acttttattc cgtgaaatca tagtattcct tatttcctcc tggatttagt
     6721 agtaataaga atagtccggc agccataaat aatgcgatca taattaaatg aataaagctc
     6781 gaccatatat aactaaaggt actttcacgg taccacggac taaacactag tgagtaatca
     6841 attacataaa tgataattgc taacactatc ggtacaattt taccttttct cgcaaaatga
     6901 tacaaaagaa ataaaagagc gctgcttacc attgtcgtta agcctaataa aaagatgcgc
     6961 atcccaattt ctaagacact attttcaatg taacgcgaaa aaatcgcaat actgtttaat
     7021 ccccagtggt aatacgtata atcactactg atgccaaaga aattaccaat taagttacta
     7081 aaaatgatta ataataaaaa aaagttaatt aacttaaaaa cgatcttttt cggatttatt
     7141 cgtgacttct ctttcatcaa tataatgctt aaccgacatc gattacttca acatcaatgt
     7201 caaacggaat taattcggtc atttgcgtta aactaagcgc gacattttca cggacttcat
     7261 ttgcaacttc ttcagcattt gtattcggac taacttttac ggaaatcttc acattaacag
     7321 taccactacg gatcgtacaa cgtactggtt tagaaagcga ataacgttta cgtgaaaccg
     7381 ttgccccact aacttcatta gtagcaatct cggcaatttt atcgaatact tgatagctaa
     7441 tacccattat gccttgctta ttgtattttt gaatgtaaac atactctgcc ataaataacc
     7501 tctggcttta attataacga aacaaacacc tttttacaat atttgttgct tttgaccaat
     7561 aatttattat tgaatgataa ttgaataagt taatttaaca atttaaaaaa taaattttat
     7621 atagtgagaa ttataaaatg gcgcatattt gcaatgcgcc attctaagta atccaattaa
     7681 aattaactac taataaacta ctaattgatt attttttagt tgttttcttt gtttttggtt
     7741 ttgcttcagt ttttacttcg gcctttgctt cagttgcttc ttgtttttct tttgtttcac
     7801 tagcggttac ttcaggttca actttttctg aaactttaag ttcaacatct tctagtgtta
     7861 aaagttcttc accttccgct ggggtgtcaa gatcacttaa aacgggcggg attggattag
     7921 gtgcttcacc ggaatagagt tcttcacctt ttttaagtgt ctttggatca ataatccctt
     7981 caacaaggcg tgcgagcatt tctggtgtat attttaagtg ctctaaaata tctttttgcg
     8041 gggcactaaa gccgaagcga tctaagcaca tcgtatgatc tgcccattcg tgccacccaa
     8101 agctacttaa gatttcaaca gcgatgcgtt tatctttgcc aagtggtaat acttcagctt
     8161 tataagcgtc atcttgacga cggaacactt ccatgctcgg catcgagaca acacggacat
     8221 tataaccgtg gatgagtaaa gtattttgcg cttttaaggc catatttact tcactacccg
     8281 ttgcgataat aataaaatca agtttctttc gtttattttc agggcttaca acataagcac
     8341 cacgacggat tagcttttct ttatcactac cagccataag tggtaatttt tgccgtgtta
     8401 agattaaggc gtgtggccgc gttttgctct ctagggctac tttccacgcc acaaatgttt
     8461 cgcgtgcgtc acccggccgc cataagtcaa agtttggcat tgcgcgcatc atcgctaagt
     8521 gttcaaccgg ttgatgggtt ggtccgtctt ctcctaaatt cacactatca tgactaaata
     8581 aataaatatt tggtaagtga cttagtgcag caagacgcat tgagttttta aggtaatcac
     8641 taaagacgag gaatgtccct acataagtgc gtagtccgcc gtgaaggagc atcccgttac
     8701 taatcgctcc catcgcgaat tcacgaatcc cgaaattgat attccgtcct ctacggtttt
     8761 tagccgtaaa gtttgtttca ccgttaatta gtgtcattac actactagca acgtcagcac
     8821 ttccgcccat aatatgcggt aaatgacgat taacagcagc gagtacatta ccactagtgt
     8881 tgcgtgttgc ttcacttaat tcgtcttcaa atgtaagttt catcattgct agactacttt
     8941 cagtatcatc gtttaaggcg atttttaagt cttcggcttc aacatcactg tcggcttcaa
     9001 ataatgtata actctttgcc caggtgtcat acgccttttg gccgttacgc gcgatttggc
     9061 ttcgcatact ttcatatact ggaagcggga tttcaaatgg cgcatttgga tagtgataag
     9121 tacctttagc aaaagtacca tctaattcac caagtggtga gccgtggact ttatgtgtac
     9181 cttgttgccg actaccttga ccgataatcg ttttcataat tacaagtgtt ggtttatcac
     9241 tgcgtttggc ttttttaatt gctttatcaa tttgtttaag attattaccg ttatcaactt
     9301 ttaaaacatt ccagccactt gctttaaagc gagcttcaac attttcacta aatgaatcgc
     9361 tcagtggtcc gtcaagcgtc acatcgttag cgtcataaaa cacaattaat ttattaagtt
     9421 tttgatgacc agcaaacgag actgcttctt gactaattcc ttcttgtaaa cagccatcgc
     9481 ccactaaaca gtacgtataa tggcttaaaa gatgcgcata ctttggatta cgcgctgtta
     9541 aactagcttc cgctagcgcc atccccacgg cattcgcaag tccttgaccg agtggtcctg
     9601 ttgtggcatc gactcctggt gtcattttat attcagggtg gcctggggta atcgagttaa
     9661 gttgtctaaa ggcttttaag tcatcaattt caatggcgta acctgctaaa tggagcacag
     9721 tataaagtaa ggcactcgca tgtcccgcac taagcacaaa acggtcacgg gcaatccatt
     9781 ctggattccg cggatcaatt ttcaaatgat ttttataaag attataaact aatggggcac
     9841 tcccaatcgc catcccaggg tgtcccgaat tagcggtatt agtaatgtca ataacagtcg
     9901 cacgaatcgc cgcgacactt aaggcatcgt aagtattacg ttttcttaaa cacataaatt
     9961 attcctctcc tccatctttt cctgtattta ttttaatata taattcatct aattgtaaat
    10021 tagaaacttt tgaaggttca ccgctcatta aacttgcgcc ctttaaattt ttggggaagg
    10081 caattacatc acgaatatca tcagtacccg ttaaaatcat cgtcagtcta tctaacccaa
    10141 aagcaatccc gccgtgcggt ggggtgccgt attttagtgc ttcgataaag aaaccgaata
    10201 aatactcaat ttctttttca cttaagccta agatttcaaa gatacgattt tgtaaatctt
    10261 ggtcataaat ccgcatactt ccgcccccag cttcataccc attaataacg atgtcataag
    10321 cataacttaa aacttcttct ggtgctgtcg ttaatttagc aatatcttct tcttttggtt
    10381 gcgtaaaggg atggtgctca ctagcgaagc gacgagtttc aggatcatag ctaaaaagtg
    10441 ggaaggaatc gacccataaa caggagtaag cattttcatc aattaagtta aagcgaacgc
    10501 ctaattcttt tctaactgcg cctagtgcac ttaaaatacg cgcgagcggg gcatcaaacc
    10561 caagtaagac gacatcattt gccgctaaac ctaaagcctc tgctacttca gtgtggctgc
    10621 tagtaatgtt ttttgcaaga ctaccgctaa aagcggtgcc gtcatacttt aaaacaccta
    10681 agaaattaac cccgtgttgc ttaacgatta agttaagttc atcaagtgtt ttacgcgata
    10741 agatgtttgt cgggtcatta ataatgagac cacgaacatc ttcttttgct ttaaaatatt
    10801 cttcattact taaaacttta cttaaatctc taatttttaa atcaaaacgt aaatcaggtt
    10861 tatcactacc ataattttct acagcttcgt gataagttaa gcgtggtaag ggcaatttta
    10921 aatcatagcc tttcacttct ttaaagattt tttgtaaaag tctttcattt aaatcaagta
    10981 cttcttcgta agtaaagaaa cttgtctcaa tatcaatttg ggtaaaatca ggttggcggt
    11041 cactacgtaa gtcttcgtct ctaaagcagc gggcgatttg ataatacttt tctagcccac
    11101 caatcattaa aagttgttta taaatttgtg gtgactgcgc tagggcgtag aaagtgcctg
    11161 ggtttaagcg tgaggggatt aaatagtcac gagcaccacc aggtgtactt ttagttaaaa
    11221 ttggtgtttc aatttcgata aacccttcat tatctaaaaa ttcacgcgtt actttcgtta
    11281 ttttagcgcg ggcgataagt ttatcttgta atactttacg ccgtaaatct aaataacgat
    11341 attttagccg cacatcttct aaagcgtctg tctcatcggc aataataagc ggcgggttct
    11401 tagctttatt aataagtgtg actttacttg ccacgacttc aactttccct gtctttaagt
    11461 ttttatttgg cacttcttta agcgtgacaa cgccttcgat ttgaataatg tattcattgc
    11521 gaatatcagg gcgcgctacg ccttcgttta ttaacacttg gacaatcccg cttcggtcac
    11581 gtaaatccac aaatgttaag ccgcctaaat cacgtttttt agcgacccaa ccaatgattt
    11641 taactgtttg tccaactagt ttctcattaa tatttccgtt ttcaattgtt cgatacataa
    11701 gtttatgcct tctcttcttc tttttcttca tgttttttgc agttcacacc acgaccttta
    11761 cctttgccag tgcattcatg atttttttca tgttcatgtt tacggcagca ttcatgcttt
    11821 tcatcgtgat gatgttcgcc gcagcattca ccgttttctt tatgagagtc gcattcacac
    11881 tcttcatggt cacagtcgca atcctcatgt tcacagccgc atttacattt atgatcatga
    11941 ttatcacaat cgcaatcttc atggtcacac ccacaattac aggcgtgata ttcttcacaa
    12001 aggtcaagta tataattaat cacttcggca aacggtactt caacttgctc ttgtgtttct
    12061 aagttccgta cactgacaac ttcattttta atttcagctt cgccgagaat taaagcaaca
    12121 cgtgctccac gattttcggc gattttaaag agtgatttaa atgatttatc ttcaaaattc
    12181 atgattccgc gtagcccgtt cgcgcgtaaa gcagttagta aactataacc accagtccgt
    12241 gcttcttcac cgatcgcgac aaaaataaag tcatctttaa ctgcggtgtg ctcaagtttt
    12301 ccttcttcat ttaaaatgtg atagagccgt tcaaggccaa aagcaaaacc aacaccttct
    12361 aaacttggac cgcctaactc ttccacaagt ttatcatagc ggccaccgcc accaatcgct
    12421 ccgtaatctt tacctgaagg cgggatatag acaaattcaa agataatgcc tgaataataa
    12481 tcaagtccgc gcactaatcc gtaatcaact tccacattaa caccatattc ttccagtgaa
    12541 gttttaatga attcaaggtc ttttttatct tcttcagtta agaaatcagt aacttttggt
    12601 gccgctttgg caagttcgac gtcacttggt actttacaat caagcatccg tagcggattt
    12661 tgttcataac gcactttaca atcaggacat acatccttta aatgtggttc ataatatttt
    12721 ttaagtgctt cgcgataggc gtcacgcgct tcttgcttcc caagactatt aattttagtt
    12781 gtaatatttt caagtcctaa agcttgtaaa gaatggattg caagtaaaat tacttcaaga
    12841 tcagcaataa tgctcgtact accaacactt tcaaccccaa attgatggaa ttgtcgataa
    12901 cgcccaagct gtggtctttc atagcggaac attggtccac tgtagtaata acgcagcggt
    12961 aaatcattcg tagcgtaaag cttattagca ataatgctac gcattactcc tgcggtacct
    13021 tcaggacgca gtgccattaa gcggttacct ttatcggtaa atacatacat ttctttccgt
    13081 acaatgtctg tcgcatcacc gacaccacgg gcaaatagtt cattagcctc gaaaatcggt
    13141 gttgtcagtg gatcaaaacc ataaagtcgg gcaacgatgc cgcatgtatc acttatataa
    13201 gcataagcac ggtcttcgtt tccaattaag tcatgtgtgc ccttagggct cgtaataata
    13261 gccatatatc cttgtattat ctaatcatgc cccggtactt cgattaggta atacatctcc
    13321 tttc
//
LOCUS       DAOSTA010000024        13209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_4743_length_13209_cov_12.574958, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000024 DAOSTA010000000
VERSION     DAOSTA010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 13209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13209
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4743_length_13209_cov_12.574958"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..419
                     /gene="rpsH"
                     /locus_tag="PLU08_03305"
     CDS             24..419
                     /gene="rpsH"
                     /locus_tag="PLU08_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOQ70754.1"
                     /translation="MSMTDPIADMLTRIRNANALSHEAVAMPSSKNKVNIARILKEEG
                     YITDYVVSGDVKKVLTLVLKYGPNGEKVISGLKRISKPGLRVTTTSDKLPRVLRGLGI
                     AIISTSQGIMTDKKARALGVGGEVVAYVW"
     gene            430..978
                     /gene="rplF"
                     /locus_tag="PLU08_03310"
     CDS             430..978
                     /gene="rplF"
                     /locus_tag="PLU08_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOQ70755.1"
                     /translation="MSRIGLKVIKVPAGVEVKLEGDLITVKGPKGTNHVTLPLHITLD
                     QSHEGEIHVKRDNELKQTKQSHGTTRALLHNAIVGAHEGFTIRLQIVGIGYRAIQEGE
                     SVRLEVGYSHPTVIAPCVGAKITVENPNNVVVTGYDKQAVGQTAALIRGVRRPEPYKG
                     KGIKYSDEVIIRKEGKRAGAKK"
     gene            989..1345
                     /gene="rplR"
                     /locus_tag="PLU08_03315"
     CDS             989..1345
                     /gene="rplR"
                     /locus_tag="PLU08_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOQ70756.1"
                     /translation="MVKQIKRNEIRKRRHARIRRKISGTHEVPRLVVFRSNKHIAVQL
                     IDDTKGVTLGSASSLAMKLVHGDNVESAAKVGTEIANVAKNLGITKVVFDRGGYLYHG
                     RIKALADAARKAGLEF"
     gene            1359..2003
                     /gene="rpsE"
                     /locus_tag="PLU08_03320"
     CDS             1359..2003
                     /gene="rpsE"
                     /locus_tag="PLU08_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016340481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOQ70757.1"
                     /translation="MDKEKKNLVEDTEEVETEEVETEEKDTEQTPQGDAPRRSNRGGR
                     REGGPRRGGRRAPARRPKEFEERVVAINRVSKTTKGGRRLRFTALVVIGYGKGKYGYG
                     NAKAAEVPDAIRKAIEVAKKNTFTVELVKGDTIAHEIVGRFGATRVFLKPAPEGTGVI
                     AGGSVRAILELAGIRNVYSKVYGSRTALNVLKATDNGLRGLKRASDIKRLRGTL"
     gene            2014..2454
                     /gene="rplO"
                     /locus_tag="PLU08_03325"
     CDS             2014..2454
                     /gene="rplO"
                     /locus_tag="PLU08_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOQ70758.1"
                     /translation="MKLHELTYTEGAKRARKRLGRGIGSGTGKTSGKGEKGQKSRSSG
                     GTRLGFEGGQMPLYRRIPKRGFTNMNTRKFAVVNLEELNVFEDGTVVTPTLLIEKGII
                     KQELGGVKVLARGELTKKLTVKAHRFSKQASDAIAKLGGTAEVI"
     gene            2456..3802
                     /gene="secY"
                     /locus_tag="PLU08_03330"
     CDS             2456..3802
                     /gene="secY"
                     /locus_tag="PLU08_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOQ70759.1"
                     /translation="MKKIAAIFKNKEIVDRIFFTIMIMFVFRIGAAITVPGVTLSDFS
                     FQNNDAFGLMNLLGGGTLQQFSVFALGVSPYITASIIVQLLSMDVLPALTDLANQGDY
                     GRKKMEMTNRYLTLMLGAVQAYGVIVTMENQSYITLKESNFWTYAYIITVMLAGTMLV
                     MWLGDQISSKGIGNGISMIIFAGIVSSLPQQIIRAYQNWLSDKVGQGSQQLFIGLLQF
                     GVYVLSFLAIIGFITFIETSKRKIPVQHAGKSGQTRKMAQASFLPIKINSAGVIPVIF
                     ASSIMMAPSIIASFISGSNLDAEWLKIFNSSATVTMPWFNDKTWQMPWGLLIYLFLIV
                     VFAFFYAKLQINPEKLAENFQKNGSYIPGVRPGAETERYVSKVLNRITVIGAFSLAVI
                     AALPIILVLLKVVPDQSLALGGTGLIIVVGVAIEINNQIDGLLAGKSYEEGSVLGG"
     gene            3807..4466
                     /locus_tag="PLU08_03335"
     CDS             3807..4466
                     /locus_tag="PLU08_03335"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOQ70760.1"
                     /translation="MCNIILLGPPGAGKGTLAKQLISVYNIPHISTGDMFREAIKEGT
                     PLGRLAASYINEGHLVPDEVTISLVRERLSQGDCANGYLLDGFPRTLPQAEALDTIGK
                     DLGRPINVVINITADETELVRRISGRRVCRKCGAPYHIDTMKPKKDGVCDACGGELYQ
                     RKDDNKDNLLVRLNHYFADTKPLEDFYAEHGVLYAVDSTAGAKKSLKAAQKIIEGAKC
                     D"
     gene            4459..5217
                     /gene="map"
                     /locus_tag="PLU08_03340"
     CDS             4459..5217
                     /gene="map"
                     /locus_tag="PLU08_03340"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOQ70761.1"
                     /translation="MISQKSPREVRLMKESGRIINLVFKEVEAKLRPGMSTADVDKLA
                     LEVITREGGTPTFKGYGGFSGNICVSVNDTLIHGIPSHREIIKDGDIVSVDVGVTKDG
                     YVADAARTFLVGNVSENAKRLVEVTEESFWYAVDNFAKPGNYIGDISHAIYEYASSRG
                     YTLTRDYTGHGVGRKLHEAPAVPNVGTPKTGPRLKVGMTLAIEPMLNEGLVDLIVLKD
                     GWTVKTKDGKLASHYENTIVITEDGYEVLTMEEK"
     gene            5217..5435
                     /gene="infA"
                     /locus_tag="PLU08_03345"
     CDS             5217..5435
                     /gene="infA"
                     /locus_tag="PLU08_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOQ70762.1"
                     /translation="MSKSNVITVEAKVIECLPGVNFRVQLDNGHIILAHVSGKIRRYN
                     IRILPGDRVAVEISPYDLTRGRITYRYR"
     gene            5458..5571
                     /gene="rpmJ"
                     /locus_tag="PLU08_03350"
     CDS             5458..5571
                     /gene="rpmJ"
                     /locus_tag="PLU08_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOQ70763.1"
                     /translation="MKVRASVKPICDKCRVIRRKGNVMVICENPKHKQRQG"
     gene            5583..5960
                     /gene="rpsM"
                     /locus_tag="PLU08_03355"
     CDS             5583..5960
                     /gene="rpsM"
                     /locus_tag="PLU08_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOQ70764.1"
                     /translation="MARIVGVDIPNDKRVVVSLQYIYGIGLTTAKKILAAVKIDENVR
                     VKDLTEEELGAIRQEITKYRVEGDARRQVALNIKRLTEIGSYRGIRHRRGLPVRGQRT
                     KTNARTRKGPKKTIANKKQASQG"
     gene            5981..6379
                     /gene="rpsK"
                     /locus_tag="PLU08_03360"
     CDS             5981..6379
                     /gene="rpsK"
                     /locus_tag="PLU08_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOQ70765.1"
                     /translation="MSKKARRSTTRRRRIRRSYTACIAHIHSTFNNTIVTITDEDGNA
                     ISWSSAGALGFKGSKKSTAWAAQLAGEAAAKVAVEQGLKTVDVRVKGPGQGREAAIRA
                     LALAGLTITGIEDTTPIPHNGCRPPKRPRG"
     gene            6401..7366
                     /locus_tag="PLU08_03365"
     CDS             6401..7366
                     /locus_tag="PLU08_03365"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003641267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOQ70766.1"
                     /translation="MIPFNPTVFKEKNYYATDNYGKFVLEPLERGFGQTLGNSFRRLL
                     LSALPGASVFAITIDGARHEFATLPGVVEDVTMITLNLKQLILKIEDKDTTIKRLEVD
                     VVGPAVVTGADITLPYGVEVISKDLVIANVAEGGHLKMTIFARNGRGYVTSESNKAER
                     QKVGGAIGVIATDSNYSPVTKVNYTVSNTRVGHDENYDRLTLEVWTNGSITPQAAVAL
                     SAKILIEHYKPFLDLEEATVDVEVFAEQEAQAPAQMEDKKIEELDISVRSYNCLKRAG
                     IQTVLELTSKTEEEILKVRALGKKSFKEIKDKLKELGLNFRGSVE"
     gene            7376..7777
                     /gene="rplQ"
                     /locus_tag="PLU08_03370"
     CDS             7376..7777
                     /gene="rplQ"
                     /locus_tag="PLU08_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002818480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOQ70767.1"
                     /translation="MPAQGRKNVRGKAGVRFKAAYTKSKDKAMIRNVTSELIVYGKVT
                     LIAKVGKQVQREVERLITLAKRGDLHARRLAAARLREGIKRADGVKALDVLFNELGPR
                     FKDREGGYTRILKLGPRRGDNAPMVILEFVE"
     gene            7918..8460
                     /locus_tag="PLU08_03375"
     CDS             7918..8460
                     /locus_tag="PLU08_03375"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOQ70768.1"
                     /translation="MRISINTKVSFGVTVFTSVALIIIGAMIVKYNTNLAASLFYLIG
                     IIIALVGFINFLNYISGRKQKVTVSEYLLSILLMIAGILVAIFSESIQAYGLLVIGIL
                     FVMLGIYDIVLYFRSKATITLIVGILRIVIGTAFIISGFSEVLLANDEFSRKLWLIIG
                     YASIVLGVTFLVLDTFQVTE"
     gene            8463..9125
                     /locus_tag="PLU08_03380"
     CDS             8463..9125
                     /locus_tag="PLU08_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOQ70769.1"
                     /translation="MNYKHIIFDLDGTLLDTIPDLLYNLNTTFSELGLPGKFSTAEMA
                     TFLGSGKDEQIRRALSARRLSHEKYFAKVNALLSVYYAKNTNTYTEIFPGIKETLAEL
                     KARCVKLYVATNKPDHVAKDVVRHYFGDNVFIKVRGDKGDGIIKPNPKFLNAIIKNIN
                     DPLETIMFVGDSYVDYMSAKNAGVKCAIVPHGYDQKVLNIKDKGVIFLKKVTDIISLD
                     IN"
     gene            9172..10506
                     /gene="cysS"
                     /locus_tag="PLU08_03385"
     CDS             9172..10506
                     /gene="cysS"
                     /locus_tag="PLU08_03385"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOQ70770.1"
                     /translation="MKILLYNSLTKQKEEFIPLRKGEVSMYVCGPTVYDYVHVGNMRP
                     VVVFDTLRRFFNHAGYQVIYVSNYTDVDDRIIEKAQKTNKKETEISEFYIEAFAESAT
                     KINALKPTFTPKATQHIESMIAFIKVLIANGAAYENDGEVFFRVSKVETYGELSNVTQ
                     DELQVGARIDANPKKEHPNDFLLWKKTDDAGIKWDSPWGAGRPGWHTECVVMINDIFG
                     KPLIDIHGGGFDLKFPHHENEIAQARAYANTGLANYWMHNGFINLNNQKMAKSTGNFV
                     TAKDFIEEYGATSLRLLLLSTHYRSPVNLSAEIIENTNNEVLRLQRTKQALVRHFALH
                     PLKVTDDSLLLKFEEALADDLNTPNALSELYAVVKDLNILLRNPLKEEEKLAKLANAF
                     EVMLDVLGLDLGVKNLNEEDTKLLKDYENARKKKDFAKSDALREELQKRGIF"
     gene            10506..11237
                     /gene="rlmB"
                     /locus_tag="PLU08_03390"
     CDS             10506..11237
                     /gene="rlmB"
                     /locus_tag="PLU08_03390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOQ70771.1"
                     /translation="MNNNNQRTQLAYGRNNVLACLDAGIVIKIIFQEGFSDEKIIKLA
                     TKQNLEITYAKKKDLNNMVAGNHQGIVAVVKSFKFNILEELIIAANNVDNPIILVLDE
                     IKDPHNLGAIIRSAEAFGVTGIVMKKDRQVSITPAVMKVATGAQNNILIAQVTNLSQA
                     LEKLKKTGFWVVSTDLKAEQNVSDLKYDFKTALIMGNEHKGVSPLLTKNSDFVVKIPM
                     LGKTNSLNVSVATGVMLALIRQIQL"
     gene            11286..11897
                     /locus_tag="PLU08_03395"
     CDS             11286..11897
                     /locus_tag="PLU08_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70772.1"
                     /translation="MSLKFSTSNESAIRAYIAGDKEAINLILANFQYFIRSRAGLLAS
                     LHDKSGIDYFDLELIGQSALITAVRTYRADATPFAPFATVVINNAMSNYIKQQTSLTN
                     SLSRYAISLDTTLYDDNDNFMISDSIAEVVADNDLGIYSPAKIGFFEDLLPASLTPLE
                     IVIVKGKLAGYTYQELQKMLKLPKRKIDATIVSIKEKWRALLL"
     gene            11998..13077
                     /locus_tag="PLU08_03400"
     CDS             11998..13077
                     /locus_tag="PLU08_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOQ70773.1"
                     /translation="MKKDPFIEYLKENNPTEKQKKYSWYTAIGLQEVDGLETSDYLKE
                     TAIKNIKGEISLDETKRLIFAYYEHATEREKQNEEADKVSIQIAEILSKKAFTFSVVE
                     FLKTHKFLFLDIYEHAGEIRKFNISKKEWILNGASVTYGDFSSLKDMLEYDISQEKSV
                     DYSKFSRDERIKHIAKFIADLWQIHPFAEGNTRTTAVFLIKYLRSLGFDVTNDLFAKN
                     SWYFRNALVRANYNNVSKEIFETTEYLELFLRNLLYNENNKLSNRQMHVEYMKKPDID
                     KEKPDIDKEKPDIALKRMSLTVKTKANIMRLNDSIKNDIFGRKEIMHILGLSASRSSE
                     LIKQMLDCDIIVAVKGQGKGKYIFK"
BASE COUNT         4334 a         2327 c         2850 g         3698 t
ORIGIN      
        1 gctggtaagg aggagcaact agtatgagta tgacagatcc aattgcagat atgctaaccc
       61 gcattcggaa cgctaatgct ttaagtcacg aagcagttgc gatgccgagc agcaaaaaca
      121 aagttaatat tgcccgtatc ttaaaagaag aaggttatat tactgattac gtcgttagtg
      181 gcgacgttaa aaaagtttta accttagtgc ttaaatacgg accaaatggt gaaaaagtaa
      241 tttctggttt aaaacgtatt tctaagccag gcttaagagt caccactacc agcgataaat
      301 taccacgcgt tttacgcggt ttaggtatcg ctattatttc cacatcacaa ggaatcatga
      361 ctgacaagaa agcgcgcgcc ttaggcgtcg gtggcgaagt agtcgcctat gtgtggtagg
      421 aggtaagtta tgtcaagaat tggtttaaaa gtaattaaag tgccagcagg cgtagaagtg
      481 aaacttgaag gtgacttaat caccgttaaa ggaccaaaag ggacaaatca cgttacctta
      541 ccactccata tcacccttga tcaaagtcat gaaggtgaaa ttcatgttaa acgcgacaat
      601 gaattaaaac aaacaaaaca aagtcatggt acaacccgtg ctttactcca caatgcaatt
      661 gttggagcgc atgaaggttt tactattcgc ttacaaatcg tcggtatcgg ttaccgcgct
      721 attcaagaag gcgaaagtgt ccgcttagaa gtcggttaca gccatccaac tgtaattgct
      781 ccatgtgttg gtgctaaaat tacagtggaa aacccaaaca acgttgttgt gacgggttat
      841 gacaaacaag ctgttggtca aaccgcagcg ctcattcgcg gtgtccgtcg tccagaacct
      901 tataaaggta aaggaattaa atatagcgac gaagttatta ttcgtaagga aggaaagcgc
      961 gcaggcgcca agaaataggt gaaaagttat ggttaaacaa attaaacgta atgaaattag
     1021 aaaacggcgt catgcccgta ttcgccgcaa aattagcggt acacacgaag ttccgcgcct
     1081 tgtcgtcttc cgttccaaca aacacattgc tgtgcaatta attgatgaca ctaaaggtgt
     1141 taccttaggt agcgctagtt cattagcgat gaaattagtg catggcgaca atgttgaatc
     1201 agcggcaaaa gtcggtactg aaattgctaa tgttgcgaaa aacttaggca ttactaaggt
     1261 tgtgttcgat cgcggagggt atttatacca tggtcgaatc aaagccttag ccgatgctgc
     1321 ccgtaaggcc gggttagaat tctagaagga gtaaagttat ggataaagaa aagaaaaact
     1381 tagtagaaga tactgaagaa gttgaaactg aagaagtaga aactgaagaa aaagataccg
     1441 aacaaactcc gcaaggtgat gccccgcgcc gtagtaatag aggtggccgt cgtgaaggtg
     1501 gtccgcgtcg tggtggccgt cgtgccccag cgcgtcgccc aaaagaattt gaagaacgcg
     1561 tcgtcgcgat taaccgcgtt agtaaaacaa caaaaggtgg ccgtcgctta cgctttactg
     1621 ccttagtcgt tattggctat ggtaaaggga agtatggcta cggaaacgct aaagcagcag
     1681 aagtacctga tgcgattcgt aaagcaattg aagttgcaaa gaaaaacacc ttcacagttg
     1741 aacttgttaa gggtgacaca attgctcacg aaatcgttgg tcgcttcggt gcaacccgcg
     1801 tattcttaaa accagcacca gaaggtactg gagttatcgc cggtggttca gtgcgggcca
     1861 tcttagaact tgcaggtatt cgcaacgtct attcaaaagt ttatggttcg cgtaccgccc
     1921 ttaatgtgct taaagcaaca gataacggat tacgcggttt aaaacgagct tcggatatta
     1981 aacgtctccg gggaacattg taggaggtag agtatgaaat tacacgaact tacttataca
     2041 gaaggagcaa aaagagctcg taaacgtcta ggccggggga ttggctctgg cacaggtaaa
     2101 acaagtggaa aaggtgaaaa aggtcaaaag tcacgctcaa gcggtggcac gcgtctcggc
     2161 tttgaaggtg gacaaatgcc tttatatcgc cgtattccca aacgtggctt tacaaacatg
     2221 aatacacgaa agtttgccgt cgttaattta gaagaattaa atgtctttga agacggaact
     2281 gttgtgaccc caacactctt aattgaaaaa gggatcatta aacaagaact cggtggcgtt
     2341 aaagtcttag cgcgcggaga attaacaaag aaattaacag ttaaagccca cagattttcc
     2401 aaacaagcta gtgacgccat tgctaaacta ggtggaaccg cagaggtaat ttaatatgaa
     2461 aaagatcgcc gcgattttca agaataaaga aattgtcgat cgtatcttct ttacgatcat
     2521 gattatgttt gtcttccgaa ttggcgccgc gattacggtt ccaggcgtca cgcttagcga
     2581 cttttcattc caaaataacg atgcctttgg gcttatgaat ctccttggtg ggggcacact
     2641 gcaacaattt agtgtgttcg cacttggggt tagtccttat attaccgcat caattatcgt
     2701 ccaactctta tcaatggacg tccttcctgc gcttactgac cttgctaatc aaggggatta
     2761 tgggcggaag aaaatggaaa tgacaaatcg ttacttaacg ctgatgctcg gcgcagtgca
     2821 agcttatggc gttattgtta cgatggaaaa tcaaagttat attacgctga aagaatcaaa
     2881 cttttggaca tatgcttaca ttattaccgt catgcttgcg ggcacgatgc ttgtaatgtg
     2941 gcttggtgac caaattagta gtaaagggat tggtaatggt atctcgatga ttatcttcgc
     3001 aggtatcgtt agcagccttc cccaacaaat tattcgtgcc taccaaaatt ggcttagtga
     3061 taaagttgga caaggcagtc aacaattatt tatcggtctc ctccaatttg gagtttatgt
     3121 acttagtttc ctcgccatta ttggttttat tacctttatt gaaacgagta aacgtaaaat
     3181 tcccgtgcaa cacgcgggta aaagcgggca aacccgcaag atggcgcaag caagtttctt
     3241 accaattaag attaatagtg ccggggttat ccctgtcatc tttgcttcaa gtattatgat
     3301 ggcgccgtca atcattgctt cttttatcag cggctcaaat ttagacgccg aatggttaaa
     3361 aatctttaac tcaagcgcga cagtaacaat gccgtggttt aatgataaga cttggcaaat
     3421 gccgtgggga ttactcattt acttattcct tattgttgtg tttgccttct tctacgctaa
     3481 actacaaatt aatccggaaa agctcgcgga aaacttccaa aagaatggta gttatattcc
     3541 tggtgttcgt ccgggtgctg aaacggaacg ctatgtgtct aaagtactta accgtattac
     3601 cgtcattggt gccttcagtt tagcggttat tgcggcccta ccaattatct tagtcttact
     3661 taaagtcgtt cctgaccaaa gcttagcctt agggggaaca ggactaatca ttgtcgttgg
     3721 ggtggcaatt gaaattaata accaaattga tgggcttctc gctggtaagt cttacgagga
     3781 aggaagcgta ttaggaggtt aggcgtatgt gtaatattat cttattagga cccccagggg
     3841 ctggtaaagg aacacttgcc aaacaactaa ttagtgtcta taacattccg catatctcta
     3901 caggtgacat gttccgtgaa gcaattaaag agggcactcc acttggcaga cttgcggcaa
     3961 gttatataaa tgaagggcac ttagtgccag atgaagtaac aattagtctc gtaagagaac
     4021 gcttatcgca aggagattgc gcaaatggtt acttgttaga cgggttccca cgaaccctcc
     4081 cccaagccga agcactcgac acaattggca aagacttagg tcggccaatt aatgtagtaa
     4141 ttaatattac tgctgatgaa accgaattag tgcggcgcat cagtgggcgc agagtatgtc
     4201 gtaaatgcgg cgctccatac cacattgata cgatgaagcc gaaaaaagac ggtgtgtgtg
     4261 atgcttgtgg tggtgaatta tatcaacgca aagatgataa taaagacaat ctcttagtgc
     4321 gcttaaatca ctattttgct gatacaaagc cacttgaaga cttctatgca gagcatggtg
     4381 tcctctacgc tgttgatagt actgctgggg cgaaaaaatc actaaaagct gcacaaaaaa
     4441 ttattgaagg agctaaatgt gattagtcaa aaatcacccc gcgaagtgcg cttaatgaaa
     4501 gaatcaggtc ggattattaa cttagtcttt aaagaagtag aggctaagtt aagacctggg
     4561 atgtcaactg ctgatgttga taaactagcg cttgaagtca ttactcggga aggtggcacc
     4621 ccaaccttca agggttatgg cggatttagt ggcaatatct gtgtttcggt taatgataca
     4681 ttaatccacg gaatcccaag ccaccgcgaa attattaaag acggcgacat tgtgagtgtt
     4741 gacgtcgggg tgacaaaaga cgggtatgtt gcggatgcag cacggacatt cttagtcgga
     4801 aatgttagcg aaaacgcaaa aagactcgtc gaagttaccg aagagagctt ctggtatgca
     4861 gtcgacaact ttgcaaaacc agggaactac atcggagata tcagtcacgc aatttatgaa
     4921 tatgcgtcaa gtcgtgggta tacacttacg cgagattaca cgggtcacgg tgtggggcgt
     4981 aaattacatg aagcacccgc tgtaccaaac gttggaacgc caaagactgg tccccgctta
     5041 aaagtgggta tgaccttagc gattgaacca atgttaaacg aagggttggt cgaccttatc
     5101 gtcttaaaag acggctggac cgtcaaaaca aaagatggta aattagcgag ccattatgaa
     5161 aacaccatcg ttattaccga agatggctac gaagtattaa ctatggagga gaagtaatga
     5221 gtaaatcgaa cgtaattacc gtcgaggcga aagtcattga atgtttaccg ggagttaatt
     5281 tccgcgttca attagacaac ggacacatta ttttggccca cgtttctggt aaaatcagaa
     5341 ggtataacat tcgcattcta ccaggtgatc gtgtcgcagt tgaaatttcg ccctatgatt
     5401 taacacgggg ccgcattaca tatcggtatc gataaaagaa attggaggaa attcattatg
     5461 aaagtaagag cctcagtcaa accaatttgc gataaatgcc gggttattcg ccgcaaaggc
     5521 aatgtgatgg tcatttgcga aaaccctaag cataagcaaa gacaaggata ggaggaaaga
     5581 atatggcacg gattgttggt gtagatattc caaacgataa gcgggtcgta gtgagcttac
     5641 aatacattta tggtattggc ttaacaaccg ctaaaaaaat attagccgct gttaaaattg
     5701 atgaaaatgt tcgtgttaaa gatttaaccg aagaagaact tggtgcaatt cgtcaagaaa
     5761 ttacgaagta ccgcgtcgaa ggtgacgcca gacggcaagt agcattaaat attaaacgat
     5821 taacagaaat cggttcatac cgcgggattc gtcatcgtcg cggattacca gttcgtggtc
     5881 aacgcacaaa aacaaatgca agaacacgta aaggaccgaa gaaaacgatt gctaataaaa
     5941 agcaagcgtc acaaggttaa ggaaggagtg aaagttagtt atgtcaaaaa aagcacgcag
     6001 aagtacgaca agaagacgca gaatacgtcg ttcttatacc gcttgtatcg cgcatattca
     6061 ctcaaccttt aataacacta tcgttacgat tactgatgaa gacggcaacg ccatcagctg
     6121 gagtagcgct ggtgcattag gtttcaaagg tagtaaaaaa agcactgcat gggccgcgca
     6181 attagccggt gaagctgcag cgaaagtcgc agtggaacaa ggacttaaaa cggtcgatgt
     6241 tagagttaaa ggtccaggac aaggccgaga agccgcgatt cgcgcactcg ctttagcggg
     6301 acttacgatt accggaattg aagatacaac cccaattcca cataacgggt gtcggccccc
     6361 gaaacggcca cgcggataag gaggcaacat gagtaatatt atcatcccat tcaatcccac
     6421 cgtttttaag gaaaaaaatt attacgcaac agataactat ggcaagtttg tgcttgaacc
     6481 actcgaaaga ggctttggac aaacgctcgg taattcattc cgtcgtttac tcttaagtgc
     6541 ccttcctggt gcaagtgtct tcgctattac gattgacggc gcacgtcatg aatttgcaac
     6601 acttcctggt gttgtcgaag acgtgacgat gattacactt aaccttaaac aactcattct
     6661 caagattgaa gataaagata cgacaattaa acgtttggaa gttgatgttg tagggccagc
     6721 agtggtcact ggagcggata ttactcttcc ttacggtgtc gaagttatta gtaaagattt
     6781 agtgatcgct aatgtcgctg aaggtggtca cttaaaaatg acaatctttg ctcgcaacgg
     6841 cagaggttat gtcactagcg aaagtaataa agccgaacgg caaaaagttg gtggtgcaat
     6901 tggtgtcatc gccactgatt caaattattc accagtgaca aaagttaatt acaccgtgtc
     6961 aaacactcgt gtcggacacg atgaaaatta tgaccgctta acgcttgaag tgtggactaa
     7021 tggtagtatt accccacaag ctgcggtcgc cctcagtgcc aaaatcttaa ttgagcacta
     7081 taaacctttc cttgatttag aagaagcaac tgttgacgtt gaagtcttcg ctgaacaaga
     7141 agcacaagcg ccagcacaaa tggaagataa gaagattgaa gaactcgaca tcagtgtccg
     7201 ttcttacaac tgcttgaagc gcgctggaat tcaaacagta cttgaactta caagtaaaac
     7261 cgaagaagaa attcttaaag ttcgtgcttt aggtaaaaaa tcatttaagg aaattaaaga
     7321 caaacttaaa gaactcggcc ttaacttccg aggcagtgta gaatagaggt acaatatgcc
     7381 agcacaaggt agaaaaaatg tccggggtaa agccggagtc cgctttaaag cggcgtatac
     7441 aaaaagtaaa gacaaagcga tgattcgtaa tgttacgagt gaacttatag tttacggtaa
     7501 agtaacttta atcgctaagg tcgggaaaca agttcaacgc gaagttgaac gcttaatcac
     7561 acttgctaaa cgcggtgact tacatgcacg cagacttgcc gccgcgcggt tacgtgaagg
     7621 aattaaaaga gcagacggtg tcaaagcact tgatgtactc tttaatgaac ttggaccacg
     7681 ctttaaagat cgtgaaggcg gttatacccg cattcttaag ttaggcccac gtcgtggtga
     7741 caatgccccg atggtcatct tagaattcgt cgaataagac gcgtcttact aaaattcatt
     7801 aagcctagcc attaaggtta ggcttttttg tttaaaaagg catatcactt aaagtgataa
     7861 tactttgcaa aaagcgtgtg tttaatataa tattaaagag gaataaagga gaaaagtatg
     7921 cgcatatcaa taaatacaaa ggttagtttt ggagtaacag tcttcactag tgtcgcacta
     7981 attattattg gagcgatgat cgttaaatat aacactaatt tagcggcaag tcttttttac
     8041 ttaattggga ttattattgc ccttgttggc tttattaatt tcttgaatta tatttccggg
     8101 cgtaaacaga aagttacggt ctcggaatat ttacttagta tcttactaat gattgccgga
     8161 attttagtcg ctatttttag tgaaagtatt caagcttacg gcttacttgt cattgggatt
     8221 ttatttgtga tgttaggaat atatgatatt gttttatatt tccgctctaa agcgacaata
     8281 acactgattg ttggcatttt acggattgtt attggtacgg cctttattat ttctgggttt
     8341 agtgaagtat tgcttgctaa tgatgaattt tcacgaaaac tgtggcttat tattggttat
     8401 gcgtcaattg tattaggcgt gactttcctt gtgcttgata cattccaagt tactgaatag
     8461 ctatgaatta taaacatatt atttttgatt tagatggaac gcttttagat acgattcctg
     8521 atttactcta taacttaaac actactttta gtgaattagg tttaccgggt aaatttagca
     8581 ccgcggagat ggcgactttt ttagggagcg gtaaagacga acaaataaga agagcgctca
     8641 gtgcaagacg gcttagtcac gaaaagtatt ttgcgaaagt taatgccctt ttgtcggtgt
     8701 attacgccaa aaacacgaat acttataccg aaatattccc cggcataaaa gaaacactag
     8761 cggaattaaa agcgcgctgt gttaagttat atgttgccac taataagcca gatcacgtcg
     8821 ctaaagatgt agtgagacat tattttggtg ataatgtttt tattaaagta cgtggcgata
     8881 aaggtgacgg gattattaaa cctaatccaa aatttttaaa tgcgattatt aaaaacatta
     8941 atgacccgct tgaaacaatt atgttcgtgg gcgatagtta tgttgattat atgtcagcga
     9001 aaaatgccgg tgtaaaatgc gctattgtac cgcacggtta cgatcaaaaa gtgttaaaca
     9061 ttaaagataa aggtgtaatc tttttaaaga aagtaacaga cattatttct ttagacatta
     9121 attaggaaca tagagtgcgt taattacctg tgttcgtgat aaaatattac tatgaaaata
     9181 cttttataca actcacttac aaaacaaaaa gaagaattca ttccgctgcg taaaggcgaa
     9241 gtatcaatgt atgtatgcgg tccaacagtc tacgactatg tgcatgttgg taatatgcgt
     9301 cctgttgtcg tctttgatac attacggcgc ttttttaatc acgcaggtta tcaagtgatt
     9361 tatgtttcta actacactga tgttgatgat agaatcattg aaaaagcgca aaaaactaat
     9421 aaaaaagaaa ctgaaattag tgaattttat attgaagctt ttgcagagtc ggcaactaaa
     9481 attaatgcct taaaacctac atttacccca aaagcaacgc agcacattga aagtatgatt
     9541 gcttttataa aagtgttaat cgctaatggc gctgcttatg aaaatgatgg tgaagtattt
     9601 ttccgtgtgt caaaagttga aacttatggt gaattgtcaa atgtcacaca agacgaactt
     9661 caagttggtg cgcggattga cgctaacccg aaaaaagaac atccgaatga tttcttatta
     9721 tggaagaaaa cggatgatgc aggaattaaa tgggattcac cgtggggcgc aggtaggcca
     9781 gggtggcaca cagaatgcgt tgtgatgatt aatgatatat ttgggaagcc tttaattgat
     9841 attcatggtg gcggatttga tcttaagttt ccacatcacg aaaacgaaat cgcgcaagcg
     9901 agggcttacg ctaatacggg gcttgctaat tactggatgc ataacggctt cattaattta
     9961 aacaatcaaa agatggcaaa gtccactggt aactttgtaa ctgctaaaga ttttattgaa
    10021 gagtatggcg ctacatcatt acgcttatta cttttatcta cgcattatcg gagtccagta
    10081 aatcttagtg cagagataat tgaaaacact aataatgaag tgctgcgcct acaaagaact
    10141 aagcaagcac ttgtgcgtca ttttgcgcta cacccactca aagttacaga tgattcacta
    10201 ctcttaaaat ttgaagaagc tttagccgac gacttaaata caccaaacgc tttaagtgaa
    10261 ttatatgcag ttgttaaaga cttaaatatc ttacttcgaa acccgttaaa agaagaagaa
    10321 aaactcgcta aattagcaaa cgcatttgaa gtaatgcttg atgttttagg acttgattta
    10381 ggtgttaaaa atctaaatga agaagacact aaattgctaa aagactacga aaatgcacgg
    10441 aaaaaaaagg attttgcgaa aagtgacgct ttacgcgaag agttgcaaaa acggggtatt
    10501 ttttaatgaa taataataac caaagaacac aattagccta cggacgaaat aacgttttag
    10561 cgtgtttaga tgcggggatt gtaattaaaa ttatttttca agaaggtttt agtgatgaaa
    10621 agataattaa acttgccact aagcaaaatc ttgaaattac ttatgcaaag aaaaaagatt
    10681 taaataatat ggttgcgggc aatcatcaag gtattgtcgc cgttgttaaa tcttttaaat
    10741 ttaatatttt ggaagaactt attattgctg cgaataatgt agataatcca attattttag
    10801 tattagatga aattaaagat ccacataatc ttggggcgat aattagaagt gctgaagctt
    10861 ttggtgtcac aggaatagtg atgaaaaaag atcggcaagt ctcaattact ccagcggtaa
    10921 tgaaagttgc tactggagcg caaaataata ttttaattgc gcaagtgact aatctttcgc
    10981 aagcattaga gaaattaaaa aagactgggt tctgggttgt aagtacagac ttaaaagctg
    11041 aacaaaatgt tagcgattta aaatacgatt tcaaaactgc actgataatg ggtaatgagc
    11101 ataaaggcgt ttcaccttta ttaacgaaaa attctgattt tgttgttaag attccgatgc
    11161 ttggaaaaac taattcttta aatgtaagtg tcgcaacagg cgtaatgctt gcactaattc
    11221 gtcaaattca attgtagtta ttaatagtaa cttaaaataa attacttaat agaaaggttc
    11281 ctgccatgtc tttaaaattt tcaacatcaa acgaaagcgc tattcgtgct tatatcgctg
    11341 gtgataaaga agcaattaat ttaattcttg ctaattttca atatttcata agaagtcgcg
    11401 ctggtttact agcgtcactt catgataaaa gtggtattga ttattttgat ttagaactaa
    11461 tcggtcaaag tgcacttatt actgcggtta gaacttatag agctgatgca actccctttg
    11521 ctccttttgc caccgttgtc attaataatg cgatgtctaa ttatattaaa caacaaacaa
    11581 gcctcactaa tagcttgtcg cgttacgcta ttagtttaga tacaactttg tatgatgata
    11641 atgataattt tatgatttca gactcaattg ctgaagtcgt agccgacaat gatttaggta
    11701 tatattcacc cgctaagatt gggttctttg aagacttgct gcctgcatca ttaactccgc
    11761 ttgaaatagt aattgttaaa ggtaaactag cgggatatac ttatcaagaa ctgcaaaaga
    11821 tgttaaaact tcctaagcga aaaatagacg caacaattgt ttcaattaaa gaaaagtggc
    11881 gagcactact tttataaaat ttagaatttt gttttactcc atataatcgg caaaaattat
    11941 taattacaaa gcttaataaa tcgctttcca tatggtataa taaataagga ataatttatg
    12001 aaaaaagacc cattcattga atatttaaaa gaaaataacc ctaccgaaaa gcaaaagaaa
    12061 tactcttggt ataccgcgat aggtttacaa gaagttgatg gattagaaac ctcggattat
    12121 ttgaaagaaa ccgcaatcaa gaatataaag ggcgaaatat ctttagatga aactaaaaga
    12181 ttaattttcg catattatga acacgctact gaaagagaaa aacagaacga agaagctgac
    12241 aaagtttcaa ttcaaatcgc agaaatttta tccaaaaagg cctttacttt ttctgttgtt
    12301 gagttcttaa aaactcacaa atttctattt ttggacattt atgaacatgc aggtgaaatt
    12361 cgtaaattca atattagtaa aaaggaatgg attttaaatg gagcaagtgt tacttatgga
    12421 gacttttctt ccttaaaaga catgttggag tacgatataa gtcaagaaaa aagtgtagat
    12481 tattctaagt tctcgagaga tgagagaatt aaacacatag ctaaatttat tgctgattta
    12541 tggcaaatcc acccttttgc tgagggcaat acaagaacga cggcagtatt tttaataaag
    12601 tatttgcgtt ctttggggtt tgatgtcacc aatgatttat ttgctaaaaa ctcatggtat
    12661 tttcggaacg ctttagtaag agcaaattat aataatgttt caaaagaaat atttgaaact
    12721 acagagtatt tggagttatt tctaaggaac cttttgtata acgaaaacaa taaactttca
    12781 aatcgacaaa tgcatgtgga atatatgaaa aaaccggaca ttgataaaga aaaaccggac
    12841 attgataaag aaaaaccgga cattgcctta aaaagaatgt ctctgactgt aaaaactaag
    12901 gctaatatta tgagactgaa tgactcaatt aagaatgata tttttgggag aaaagaaata
    12961 atgcacattt tgggtttgtc tgcatctaga tcctcggaac ttataaaaca aatgttagat
    13021 tgtgacatta ttgttgcagt aaaaggtcaa ggtaaaggta aatatatttt caagtgagat
    13081 aaaccttcgt ggaattatgc tatactcttt gacaggaagt aatggctgtg ctattattcc
    13141 tttactatac acttaaaagg agtataatat atgcggaaaa agattctttt aatatgcgaa
    13201 gtttgcttg
//
LOCUS       DAOSTA010000025        12891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_4875_length_12891_cov_14.250156, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000025 DAOSTA010000000
VERSION     DAOSTA010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 12891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12891
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4875_length_12891_cov_14.250156"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..671)
                     /gene="rplD"
                     /locus_tag="PLU08_03405"
     CDS             complement(27..671)
                     /gene="rplD"
                     /locus_tag="PLU08_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOQ70774.1"
                     /translation="MSKAVKKTITYPVVNTKGEKVGTMRLNGEIFGIEPHQDVVHQAV
                     VTYLANRRQATAKTKTRAEVRGGGKKPWRQKGTGRARAGSRRSPIWVGGGVTFGPTGE
                     QNYKLSMNKKAHALALRSALSIKAAEKAFIIIDDTIEIEGKTKAAAELLKNVGATGKV
                     LLINDDNKSLLLATRNLPNVQSVGQDNVAVYDLLNADQIVVEKATLVAYVGGLE"
     gene            complement(689..1366)
                     /gene="rplC"
                     /locus_tag="PLU08_03410"
     CDS             complement(689..1366)
                     /gene="rplC"
                     /locus_tag="PLU08_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOQ70775.1"
                     /translation="MKAIIGRKMGMTQVFAEDGTIYPVTVVEVLPNVVTQVKTVEKDG
                     YAAIQVGYQDKKPSRVNKPLKGIYEKAGVTPKYGLYELKGEEMLSYEVGSEIKVDIFK
                     KGDIVDVTGTSKGKGYTGVIKRYGFKLGNAAHGSGYHRGIGSQASVGRIQRVIPGTKM
                     PGHHGAQRTTVLNLLVVDVLPEKNALLIKGAIPGPKYGILKVRSAVKKQLRKREVKDL
                     VDRTAKE"
     gene            complement(1379..1690)
                     /gene="rpsJ"
                     /locus_tag="PLU08_03415"
     CDS             complement(1379..1690)
                     /gene="rpsJ"
                     /locus_tag="PLU08_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOQ70776.1"
                     /translation="MAGKQKIRVRLQSYDHALLDEAVAKIVEQAVESGAKVVGPIPLP
                     TQREVYTVLRAVHKYKDAREQFEIRTHKRLIDIVDPNKRTVEFLRKFELPSGVDIDIK
                     L"
     gene            complement(2018..2680)
                     /locus_tag="PLU08_03420"
     CDS             complement(2018..2680)
                     /locus_tag="PLU08_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOQ70777.1"
                     /translation="MEKINYYNISIAEVNNFKDQGLRPKLALHACCAPCSSHTLEFLT
                     PFFAVTIVYQNSNIYPKAEFKKRRDEVERFVREFNIKNNQDVKVVFLPYDHEAFMKEL
                     RVFASEKEGGKRCHLCYEKRIVEALKYGSENGFDYATTTLTISRQKNSQVINAIAKKI
                     APRYPNIKYFYSDFKKKAGLLRVREMKKEYNLYQQEYCGCEYSYRDTVLARNNKKDTE
                     NR"
     gene            complement(2667..3701)
                     /gene="queA"
                     /locus_tag="PLU08_03425"
     CDS             complement(2667..3701)
                     /gene="queA"
                     /locus_tag="PLU08_03425"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004801206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOQ70778.1"
                     /translation="MDVNLFDFDLPKELIAQYPSLLRQNARLLAFNKDKNEIKDEHFY
                     DITKYFKHGDVLVINNTKVIPARLRGIKVPTGAKIEVVLLKALKDDEYEVLIGNAKAV
                     KVGTVVSFGDGELLMECREKKGEGIHIVKFKYEGVFLEVLERVGTTPLPPYIEDKSEK
                     YARYQTVYAKAPGSAAAPTAGFHFTPEILDELKTKGVIVVEITLHIGLGTFKPVSVSD
                     TKDHKMHYETYEISEEAAATINKAIEEKRRIISVGTTATRTLEANFNKYQKIVARSEA
                     TNLFITPGYKFKVISALITNFHLPKSTLIMLVSAFAGRENTLAAYCHAIKERYRFFSF
                     GDVMYIYGED"
     gene            complement(3701..4834)
                     /gene="tgt"
                     /locus_tag="PLU08_03430"
     CDS             complement(3701..4834)
                     /gene="tgt"
                     /locus_tag="PLU08_03430"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006526259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOQ70779.1"
                     /translation="MAKKPPVYLEIIHECTQSGARYGFLHTPHGKVEVPMFMPVGTLA
                     NVKTITPEEIVALGSGVILANTYHLSLRPGTDILKKAGGIHSFMNHHGPILTDSGGFQ
                     VFSLADNRKISEEGVTFKSHLDGRELFFSPEEAIRMQEEIGADIIMSFDECIHYPADY
                     EYVKASTERTLRWAKRGKAAHKREDQALFGIVQGGEYPDLRKYSAMETVKIGFPGYAI
                     GGTSIGEPKDVFIKMVESSVPYLPKDKPRYLMGIGSIDMILQGVARGVDMFDCVLPTR
                     IARHGSLYTRKGRLNIRRAEFKDDFRPIEEECDCYTCKNYTRAYLRHLYVSEEALAQR
                     LLSIHNLRFLIKLMEDVRVAIKENRFLDFKSDVLKKFGDKRGF"
     gene            complement(4827..5333)
                     /locus_tag="PLU08_03435"
     CDS             complement(4827..5333)
                     /locus_tag="PLU08_03435"
                     /inference="COORDINATES: protein motif:HMM:NF017941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOQ70780.1"
                     /translation="MKKKKIIRQITDNALIAAIYYVLTIILGGFAFNDVQFRISEILL
                     FLIFFRKDFVIGVTLGCLLANLHSLLQPWDVIFGTLATLLSGVLIAFSKRLYWGVIWP
                     TIINAVVVGLMLHIILDLPLFATMGLVAIGEFVVLLLGHAVFSLLAKQPAFLNLIMAE
                     QRKEEKDG"
     gene            complement(5391..6818)
                     /gene="gatB"
                     /locus_tag="PLU08_03440"
     CDS             complement(5391..6818)
                     /gene="gatB"
                     /locus_tag="PLU08_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOQ70781.1"
                     /translation="MKYEVIIGLEIHIETKTNTKMFSSASSLFDAPANSNVSVVDMAF
                     PGTLPTVNKKAVQNAIMLGHALNMEIDDELHFDRKNYFYSDLPKGYQITQQRRPIGSN
                     GYLDIYVNDTKKRIRIERIHMEEDTAKQIHLGEVTLIDYNRAGNPLVELVTEPDLRSA
                     DEAMAFVEAVREIVTFLEISDGKMEEGSLRCDVNISLRPVGVSKFGNKVEIKNMNSIS
                     NIGKAIETEIKRQSELLDKGLEVESETRRYDDALKDTITMRKKDESVDYRYIPEGNIL
                     PISLSKEFIDATIREMKELPSVKRERYFEEFKLNAHEVNILLQDKDLTSYFDEAARDV
                     KNYKTLINWVIGEFSSYLNKEGKNAKSVALSPQNTSKLVNLIDNGTISNSQARELFAH
                     IIETNEDPETKAKALNLMQVSDTYFILSIINEVINENLNVIDDIKAGKDRAMGFLVGQ
                     VMKKTKGKVNPALASKLMKQEIDKR"
     gene            complement(6815..8257)
                     /locus_tag="PLU08_03445"
     CDS             complement(6815..8257)
                     /locus_tag="PLU08_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOQ70782.1"
                     /translation="MRYLGLTMAELHELYVSGKATPLDVVNEVIEALTKDKNNILEKT
                     MYDEARSMAQSLTKVEKDNLLWGIPFLVKDNIAMKGIETTGSSNILNGFIPLCNAEVI
                     DKLLAAKAIPVAKTTLDEFGMGGRGETGHKGITYNPYGKERTRIVGGSSSGSASGVAS
                     GYVPFAVGSDTGDSIRKPASFAGLVGFKPTWGEISRFGLFAFMPTLDHVGFFTRSVSE
                     ARVLFEKTRGYDKKDATSVMNVPFKTEKDATLKGKKVAVLEPIFALMKNKLLIKEFHN
                     LVDKLRQAGAVVDFVPFDKDLLAAIFPTYFILSCAEANVSNACLTGIGFGNKEEGASY
                     EEIVANTRGKGFSSRIKYRLMLGQYALKKENRNEYYDRAQKARRLIVEAVDKVLENYD
                     VIMLPAAPNHAPTVDKQSEPHSEIENNFMAIANFGGNPSLTLPYFLDEGLPLGVNLTG
                     KPFDDFNVLNIALKIEEITGLYNLHAGLKS"
     gene            complement(8259..8546)
                     /locus_tag="PLU08_03450"
     CDS             complement(8259..8546)
                     /locus_tag="PLU08_03450"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70783.1"
                     /translation="MNKEEIRKLAAEILITLTPEEEATFAAEFAYFKELAKLLEALPN
                     IDEKEPMVFPFKVTQTYLKEDTPEESPGAKEILKNSKNAKDTFVVIPKVVK"
     gene            complement(8569..10560)
                     /gene="ligA"
                     /locus_tag="PLU08_03455"
     CDS             complement(8569..10560)
                     /gene="ligA"
                     /locus_tag="PLU08_03455"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOQ70784.1"
                     /translation="MDYKARILYLREVLNKYNYEYYVLNNSSVSDAEYDSLMQELIAL
                     EKAHPDTFDKTSPSQRVGGFVASEFNKITHSSMMLSLANAFSADDLRDFDRRVRGVIK
                     KDVIDYVVELKIDGLAMSLMYIDGKLSYAATRGDGEVGEDVTSNIVTIKSIPLTLKEE
                     VSFEVRGEVFMPKKSWEELNAIREETGEALFANPRNAAAGSIRQLDSKIAASRKLDAF
                     WYFLVDPLSLNIKTHYDALDYLRTLGFKVNDKIKLCKGIEEVIRHIEKVTSQRHDFPY
                     EIDGIVVKVNDVTKYDELGYTAKTPRWATAYKFPPEEVETILEDIFFTVGRTGKITPN
                     AALKPVFVAGSKIQNATLHNAAFITDRDLKIGDHVFIRKAGDIIPEVLRAVPEKRTGA
                     EKDFVMIENCPVCLEPLTFKDPLHFCLNASCPARYEEGLIHFASRGAMDIEGLGERIV
                     EILFSQRLITNIADIYTLHTKREELMQIEGFGEKSVDNLLNAIEKSKEKSLEKLVFGL
                     GIKELGAKTSKILAEKYMTLDALSCATEAELLTIRDIGPVAASSIVSYFSNEQNLALI
                     TKLKEFGLNTDYLGSVKKDNTFFSGKTIVITGKFENYSRDELTALLENLGAKVAGSVS
                     KKTSLVVAGEDAGSKLTKAQELNIDILNEEQLLKLISEA"
     gene            complement(10563..12764)
                     /locus_tag="PLU08_03460"
     CDS             complement(10563..12764)
                     /locus_tag="PLU08_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOQ70785.1"
                     /translation="MNLYENLNSKQLEAVTTSGQYVRVIAGAGSGKTRVLTTRIAYLV
                     QEIGTPEDRIVGFTFTNKAAGEMTNRLKKYLERDNVRVRLSTFHSFGARLLREDIDVL
                     GYSRSFTIYDEEDTSSLIRNISVERGHERRGELTRDANNFIGAVKEKGLDAFTYKIRP
                     HGFVNEKEFVEIWKEYERRLKASNCLDFSDLISKSITILEEYRAIREKWQHRFDYILV
                     DEFQDTNDLQYKLIKLLMHQNTSLYVVGDPDQTIYTWRGANERIIMNFDSDFKNAETI
                     ILEENYRSTKYILDAANKLIAHNKNRVEKNLFTAKEKGEPVIVQYTESDSREVNWIYE
                     QIISLRLNVKDFSFRDVAILMRANYLTLPFERHFLHKNVPYRIYGGVRFYQRREIKDV
                     LAYLRILVNEKDDISFSRIVNVPRRGMGNVAMENLKIAALKNEYSLFETVHYVEDIGL
                     SSASQEQLKALMNAVDETRTKLQNDKLELSTVINDYIQKVGYYEHIKTEKDEDKQADM
                     RANVQVLIADIKQFMNDYPESTFNDYLENASLHSAQDEVEEGDYITLMTVHMAKGLEY
                     DYIFVSGLVEGVFPNNRALLDAGRAGEEEERRLCYVAFTRARKRLYLTMSSQYNFAIA
                     SGGRPSRFISEAGLGPPKIKEYHPKYGTPLKEGGVKQVKKEAPRYRANYAEYDYASDF
                     PYAVNDIVEHKTFGSGIVTAIFPTQTTIEVKFDTGEVKRLIASPKYFKEKK"
BASE COUNT         3717 a         2704 c         2102 g         4368 t
ORIGIN      
        1 tgcttttttg cgtggacgtt tactcattat tcaagtcctc ctacatacgc aactaaggtt
       61 gctttttcga cgacgatttg gtcagcgttt aataagtcat aaacggcgac attgtcttga
      121 ccaacgcttt ggacgtttgg taagttgcgg gtggcaagta gtaagctctt attgtcgtca
      181 ttaattaata agactttacc agtggcccca acatttttaa gtaattccgc agcggctttt
      241 gttttgcctt caatttcgat ggtgtcatcg ataatgatga atgctttttc cgcagctttg
      301 attgagagcg cacttcttaa tgcgagcgcg tgcgctttct tattcattga taatttgtag
      361 ttttgttctc cggttggacc aaaggtgact ccaccgccaa cccagatggg tgaacgccga
      421 ctaccagcgc gggcacggcc tgttcctttt tgccgccatg gtttcttacc accaccgcgt
      481 acttctgcgc gtgtttttgt tttcgcggta gcttgacgcc ggttagcaag gtaagtcacg
      541 actgcttgat ggacgacatc ttgatgcggc tcaataccga agatttcgcc attaagtcgc
      601 attgtgccaa ctttttcccc tttagtgtta acaactggat aagtaatagt tttctttact
      661 gcttttgaca taactataac ctccattctt attctttagc ggtgcgatcg actaagtctt
      721 taacttcgcg tttgcgtaat tgctttttaa ccgcacttct aactttcaat attccgtatt
      781 taggacctgg aatggcacct ttaattaaga gtgcattctt ttctggtaag acatcaacaa
      841 cgagtaagtt taagactgtt gttctttgcg ctccatgatg tccaggcatt tttgttccgg
      901 gaattacgcg ttgaatacga ccaacgctag cttgcgaacc aattccgcgg tgataaccag
      961 aaccgtgggc ggcatttcca agtttgaagc catagcgttt aatgacacca gtgtagcctt
     1021 tccctttgct tgttccagtt acgtcgacga tatctccttt tttgaagatg tcgactttaa
     1081 tttcgctacc aacttcgtag ctaagcattt cttcaccctt taattcgtat aaaccgtatt
     1141 ttggagtgac acctgctttt tcgtatatgc ctttaagtgg tttatttacc cggctcggtt
     1201 ttttatcttg atagcctact tggatggcgg catagccatc tttttccact gttttcactt
     1261 gagtgacaac atttggtaag acttcaacaa ctgtaacggg gtagattgtg ccatcttcgg
     1321 cgaatacttg ggtcataccc attttgcggc caataattgc tttcatattt ctacctcctt
     1381 atagtttaat atcgatgtcg acaccacttg ggagttcaaa cttccgtaag aattccactg
     1441 tgcgtttgtt tggatcgacg atatcgataa gccgtttgtg tgttctaatt tcgaattgct
     1501 cacgcgcatc tttgtacttg tggaccgctc gtaacaccgt gtacacctca cgttgtgttg
     1561 ggagcgggat ggggccaact accttggcgc cactttcgac tgcttgttca acgatcttcg
     1621 ctactgcttc atcaagtaat gcatgatcat aactttgaag tcgaaccctt atcttttgtt
     1681 ttcctgccat gaattatttt cctccttaca tctacaggcg ctaatggtcc tcagctacga
     1741 attccccgat tacacttcat gacaacgctt gtaggtgtat cggcaacctc gcatttcaac
     1801 gcgaccatta acttgttaaa taatactaaa agcataatat aatgtcaacc ataaacgata
     1861 aaaaatatat atttttatat ataaggtcac aaaatcttgt gaaaaaccaa atttcaaact
     1921 tttgaacttt ttaccgaaaa tcgtgaaaaa aaccactttt gcaagcggtt ccattttttc
     1981 ttaaatactg ctgtttttgt ccaaaaatcg tgaaaaatca acggttttcg gtgtcttttt
     2041 tattatttct tgctaaaacg gtgtcgcgat aagagtattc acaaccacaa tattcttgct
     2101 gataaaggtt atattctttt ttcatttcac ggacacgcaa taatccggcc tttttcttaa
     2161 aatcactata aaaatattta atatttgggt agcgaggcgc aatcttttta gcaattgcat
     2221 tgatgacttg tgagttcttt tgtcgcgaga tagtaagcgt cgtagtggcg taatcaaatc
     2281 cgttttcact accatacttt aaagcttcta caatacgttt ttcataacaa agatgacagc
     2341 gtttaccgcc ttctttttca ctagcgaaca ctcttaattc cttcataaag gcttcgtgat
     2401 catatggtaa aaacacgact ttgacatctt gattattttt aatattaaat tctcttacga
     2461 atctttcaac ttcgtcgcgt cttttcttaa actcagcttt gggatagatg ttgctatttt
     2521 gatacacaat tgtcacagca aaaaatggtg ttaaaaattc tagggtgtga ctagaacacg
     2581 gcgcacagca agcatggagc gctagttttg gtctaagccc ttgatctttg aaattattaa
     2641 cttctgcaat cgaaatgttg taataattaa tcttctccat atatatacat tacatcacca
     2701 aaagagaaaa agcgatagcg ttctttaatt gcgtggcaat aagccgcgag tgtattttca
     2761 cgccccgcaa aagcactaac taacataatt agtgtagatt taggtaaatg aaaattcgta
     2821 attaacgcgc tgatgacttt aaatttataa cctggagtaa taaataaatt tgttgcttca
     2881 ctcctcgcta caatcttttg atatttatta aaattagctt ctagtgttct tgttgcggtt
     2941 gtgccaacac tgatgattct tcgtttttct tcaatcgctt tatttattgt tgcggccgct
     3001 tcttcgctaa tttcataagt ttcgtaatgc atcttatgat ctttagtatc actaacactg
     3061 accggtttaa aagtacctaa gccaatatgc agtgtaattt cgacgactat cactccttta
     3121 gtttttaact catctaatat ttccggcgta aagtgaaagc ctgctgttgg tgctgccgca
     3181 ctaccaggcg ctttagcgta caccgtttga taacgcgcat atttttcact tttatcttca
     3241 atataaggcg ggagcggggt tgtcccaacc ctttctaaga cttctaaaaa gacaccctca
     3301 tacttaaact ttacaatgtg aatgccttct ccttttttct cgcggcattc cattagaagc
     3361 tcgccgtcac caaaagaaac aacagttcca actttaacgg ctttagcatt accaattaac
     3421 acttcatatt catcgtcttt tagtgctttt aataacacaa cctcaatctt tgctcctgtt
     3481 ggaactttaa tgcctctaag tctagcggga ataacttttg tattattaat aactaacaca
     3541 tcaccatgct taaaatactt agtaatatca taaaaatgtt catcttttat ttcattttta
     3601 tctttgttaa acgctaaaag acgtgcattt tgccgtaaaa gagaaggata ttgcgcaatt
     3661 agttcttttg gtaaatcaaa atcaaaaaga ttaacatcca ttagaaacct cttttatcgc
     3721 caaacttttt taagacatca ctcttaaaat ctaaaaagcg attttcttta atcgccacac
     3781 gcacatcttc cataagttta attaaaaagc gtaagttatg aattgagagt aagcgctgcg
     3841 ctaaagcttc ttcactaaca tataaatggc gtaaataagc acgcgtgtaa ttcttacatg
     3901 tatagcaatc gcactcttct tcaattgggc ggaagtcatc tttaaattca gcgcgccgga
     3961 tattaagtct gccttttctt gtatagagcg aaccgtgacg cgcaatacgc gttggtagta
     4021 cacaatcaaa catatcaaca ccgcgcgcta caccttgtaa gatcatatca attgaaccaa
     4081 tccccattaa gtaacgcggt ttgtctttag gtaaataagg gacggaactt tccaccatct
     4141 ttataaagac atcttttggt tcaccgatgc ttgttccacc aattgcgtaa ccaggaaacc
     4201 caatttttac tgtttccatc gcactatatt tgcgtaagtc ggggtattct ccgccttgta
     4261 caataccaaa taaagcttgg tcttcacgct tatgcgctgc ttttccgcgc ttagcccagc
     4321 gtaaagtacg ttcagtactt gcttttacat attcataatc tgcgggataa tgaatacatt
     4381 catcaaaact cataataata tccgcaccaa tttcttcttg catcctaatt gcttcttctg
     4441 gtgagaagaa aagttcgcgt ccatctaaat gtgatttaaa ggtcacacct tcttcgctaa
     4501 tcttacgatt atcggctaaa gagaatactt gaaagccgcc tgaatcagta agaattggtc
     4561 cgtgatgatt cataaaagag tggattccac cagccttttt taaaatatca gtgcctggtc
     4621 taagtgataa gtgataagta ttcgccaaga tgactccgct gcctaaggca acaatttctt
     4681 caggagtaat tgttttaaca ttagcaagcg tacctaccgg cataaacatc ggcacttcaa
     4741 ctttgccgtg cggtgtatgt aaaaaaccgt atcgcgcacc gctttgtgtg cattcatgaa
     4801 taatttctag ataaacgggt ggttttttag ccatcttttt cctcttttct ttgttcagcc
     4861 ataatgagat ttagaaatgc aggttgcttc gctaggagcg aaaacacagc atgccctaat
     4921 aagagtacaa caaattcacc aattgcgaca agtcccattg tagcaaataa tggtaaatcg
     4981 agaataatgt gtaacattag tccgacgaca acagcattaa ttattgtcgg ccatattaca
     5041 ccccaatata gtcttttact aaaagcaatt aacactccac ttaataaagt agcaagcgtc
     5101 ccaaagataa catcccacgg ttgaagcaga ctatgcaaat tagcgagcaa acacccgagt
     5161 gtaacaccaa tgacaaagtc ttttctaaaa aatattaaaa ataataatat ttctgagatc
     5221 cgaaattgca catcattaaa agcaaatccg ccaagaataa ttgttaatac ataataaatg
     5281 gcagcaatca gcgcattatc tgtaatctgc cgaataatct ttttcttctt catattttct
     5341 ccttgttttg atggtggtgg ttaggcaagg aacacctcaa ctccgcttgc ttagcgctta
     5401 tcaatttctt gcttcatcag tttgctagca agagcgggat taacttttcc ttttgtcttt
     5461 ttcataactt gtccgactaa gaaccccata gcccgatctt tacctgcttt aatgtcatca
     5521 attacattta aattttcatt aatcacttcg ttaataatac taaggataaa gtaagtatca
     5581 ctaacttgca ttaaattaag tgctttcgct tttgtttcgg ggtcttcatt agtttcaata
     5641 atgtgcgcaa ataattcacg cgcttggcta tttgaaatcg tgccattatc tattaaattc
     5701 actaatttag aggtgttttg cggggataat gctacacttt tagcgttttt cccttcttta
     5761 tttaagtacg aactaaattc accaataacc caattaatta gtgtcttata gtttttaaca
     5821 tccctcgctg cttcatcaaa ataacttgtt aaatctttat cttgtagcaa gatatttact
     5881 tcatgagcgt taagtttaaa ttcttcaaaa tagcgttcac gctttacgct cggtaactct
     5941 ttcatttccc taattgttgc atcaataaat tccttgctta aagaaattgg taaaatattt
     6001 ccttcaggaa tatatcgata atcaacgctt tcatcttttt tacgcattgt aattgtatct
     6061 ttaagggcat cgtcataccg ccgtgtctca ctttcaactt caagcccttt atcaagtaat
     6121 tcactttgcc gtttaatttc tgtttcaatt gctttgccaa tattagaaat tgagttcata
     6181 tttttaattt ctactttatt accaaacttt gaaactccca cagggcgaag tgaaatatta
     6241 acatcacagc gtagtgatcc ttcttccatt tttccgtcac ttatttcgag gaatgtgacg
     6301 atttcacgca ctgcttccac aaacgccatt gcttcgtcag cacttcttaa gtctggttct
     6361 gtgacaagtt ccacaagcgg attaccagcg cggttatagt caataagtgt aacttcacca
     6421 aggtgaatct gtttagcggt atcttcttcc atgtggatgc gttcaatccg aatgcgtttt
     6481 ttagtgtcat taacataaat atctaaataa ccattactac caattggccg tctttgttgg
     6541 gtgatttgat aaccttttgg taaatcacta taaaaataat ttttgcgatc aaaatgtaat
     6601 tcatcatcaa tttccatatt taaagcgtga cctaacataa ttgcattttg cactgccttt
     6661 ttatttaccg ttggtaatgt gccaggaaaa gccatatcaa caacactaac attgctgttc
     6721 gcaggtgcat caaaaagact agaagcgcta gaaaacatct ttgtgtttgt ttttgtttca
     6781 atatgaattt ctaaaccaat aattacttcg tatttcatga ttttaacccc gcgtgtaagt
     6841 tgtaaagccc ggttatttct tcgattttaa gtgcaatatt taacacatta aagtcatcaa
     6901 acggtttacc agttaaatta acaccaagcg gcaaaccttc atctaaaaag taaggaaggg
     6961 taagtgaagg attaccacca aaattagcaa tagccataaa gttattttcg atttcactat
     7021 gtggttcact ttgtttatct actgttggag cgtgattcgg tgcagcgggg agcatgatga
     7081 catcataatt ttctaatact ttatccactg cttcaacaat taagcgacgt gctttttggg
     7141 cacggtcata atattcattc ctattttctt tcttcagcgc atattgtcct aacattaaac
     7201 ggtatttaat ccgtgaagaa aagcctttgc cccttgtatt agcaacgatc tcttcataac
     7261 ttgcgccttc ttctttatta ccaaagccaa tccctgttaa acatgcgtta cttacattag
     7321 cttcggcaca gcttaagata aagtaagttg gaaatatcgc cgctagcagg tctttatcaa
     7381 acggaacaaa gtccacaact gctcccgctt ggcgaagttt atcgactaga ttatgaaatt
     7441 ctttaattaa taatttattt ttcattaacg caaaaatagg ttctaatact gcaacttttt
     7501 tgccctttaa agttgcatct ttctctgttt taaagggaac attcattact gaagttgcat
     7561 cttttttatc gtaacccctc gtcttttcaa aaagtacacg cgcttcacta acactgcggg
     7621 taaaaaatcc aacatggtct aaagttggca taaatgcgaa tagaccaaaa cgcgaaattt
     7681 cgccccatgt tggtttaaag ccaacgagtc cagcaaagct tgcgggctta cggattgagt
     7741 ctcccgtatc actaccaacc gcaaaaggaa catacccact agcgacccca ctagcgctac
     7801 cgctactaga accaccgaca attcgtgttc tttctttacc atacggatta tatgttatcc
     7861 ctttgtgacc agtttcaccg cggccaccca tcccaaattc atcaagtgtt gtttttgcaa
     7921 caggaattgc tttagcagct aaaagtttat caataacttc agcattgcaa agcggtataa
     7981 agccgttaag tatattacta ctgcctgttg tttcaatgcc tttcattgcg atattatctt
     8041 tgactaaaaa aggaattccc catagtaaat tatctttttc aactttagta agactttgtg
     8101 ccatactgcg tgcttcgtca tacattgttt tttctaaaat gttgttttta tcttttgtta
     8161 aggcttcaat tacttcatta acaacatcta atggtgttgc tttacccgac acatataatt
     8221 catgaagttc cgccattgtt aaacctaggt agcgcatatt atttcaccac ctttgggata
     8281 acgacaaaag tgtcttttgc gtttttacta ttttttaata tctctttagc gccaggactt
     8341 tcttccggcg tgtcttcttt taaatatgtt tgtgttactt taaaagggaa caccattggt
     8401 tctttttcat caatattagg taaagcttct aaaagcttcg ctaattcttt aaagtaagca
     8461 aattctgcgg caaaagtggc ttcttcttca ggagtaagcg ttattaatat ctctgctgct
     8521 aactttctta tttcttcttt attcataatt atcctcgcta gtaatatatc atgcttcgct
     8581 aattaatttt agcaattgtt cttcgtttaa tatatcaata ttaagttctt gcgcttttgt
     8641 taatttgcta ccagcgtctt cgccagcaac aacaagtgaa gtttttttac taacactgcc
     8701 cgctacttta gcgcctaaat tttctaagag agcggttaat tcgtcacgtg aataattttc
     8761 aaacttaccc gtaatgacaa ttgtcttacc agagaagaaa gtattatctt ttttaacgga
     8821 ccctagataa tccgtgttta agccgaactc ttttagttta gtaattaacg ctaaattttg
     8881 ttcattacta aagtaagaca caattgaaga agcagcaact ggaccgatat ctctaattgt
     8941 taaaagttct gcttcagtag cgcaactaag tgcgtctaaa gtcatatact tctcggctag
     9001 aatcttactt gttttcgctc ctaattcttt aatccctaaa ccaaacacta atttttctaa
     9061 agacttttct ttactttttt caatagcgtt taataagtta tcaactgatt tttcaccaaa
     9121 gccttcaatc tgcattaatt cttctctttt agtgtgcagt gtataaatat cagcaatatt
     9181 agtaattaat ctttgtgaga aaagaatttc tacaattctt tcgccgagcc cctcaatatc
     9241 cattgcgccg cgactcgcaa aatgaattaa gccttcttca taacgcgcag gacacgacgc
     9301 atttaaacaa aaatgcagcg ggtctttaaa ggtaagcggt tctaaacaaa ccggacaatt
     9361 ttcaatcatt acaaaatctt tttcggcacc cgttcttttt tctggtacag cgcgcaatac
     9421 ttctggaata atatcaccag ctttacgaat aaaaacatgg tcacctattt ttaagtcacg
     9481 atcagtgata aacgctgcat tatgtaaagt agcgttttga atctttgaac ccgcgacaaa
     9541 tactggctta agtgcagcgt ttggtgttat tttacctgtt cttccaaccg taaagaaaat
     9601 atcttcaagt attgtttcaa cttcttcggg cgggaattta taagcagtag cccaccgtgg
     9661 cgttttagcg gtataaccaa gttcgtcata ctttgtaacg tcattaactt tcacgacaat
     9721 accgtcgatt tcataaggga aatcatgtcg ttgcgatgtt actttttcaa tatgccgaat
     9781 tacttcttca atccctttac ataatttaat tttgtcattt accttaaatc ctaaagtgcg
     9841 taaataatca agagcatcat aatgcgtttt tatatttagt gaaagcggat caactaagaa
     9901 ataccaaaaa gcatcaagct tccgactagc agcaatttta gaatcgagtt gacgaattga
     9961 gcccgcggcg gcgttacgcg gattagcaaa aagtgcttca cccgtttctt cacgaatagc
    10021 atttaattct tcccaagatt ttttaggcat aaatacttcg ccacgcactt caaaactaac
    10081 ttcttcttta agtgttaaag gaatactttt aattgtgaca atatttgaag taacatcttc
    10141 cccaacttca ccgtctcctc tagttgccgc ataacttaac tttccgtcga tatacattaa
    10201 gctcatcgct aagccgtcaa tctttaactc tacgacataa tcaatgacat cttttttaat
    10261 aacaccgcgg acacgacgat caaaatcacg taagtcatca gcactaaagg cattcgcaag
    10321 tgaaagcatc attgaactat gtgtaatctt attaaattca cttgcaacaa agccaccaac
    10381 tctttggctc ggacttgttt tatcaaaagt atcgggatgc gctttttcaa gggcaattaa
    10441 ttcttgcatt aaactatcat attcagcatc actaacactc gaattattaa ggacataata
    10501 ttcatagtta tacttattta aaacttcgcg taaataaaga attcttgctt tataatccat
    10561 ctttattttt tctccttaaa gtattttggg ctagcgatta gtcgctttac ttcgccagta
    10621 tcaaacttaa cttcaattgt tgtttgcgtt ggaaaaatgg cagtaacaat tcctgatcca
    10681 aatgttttat gttccacaat atcgttaact gcataaggaa aatcactagc gtaatcgtat
    10741 tctgcataat tagcgcgata acgcggcgct tcttttttaa cttgtttaac accaccttct
    10801 ttgagcggtg tcccgtattt tggatgatat tctttaatct tcggtggtcc taaccccgct
    10861 tcactaataa agcgtgatgg tctcccgcca gaagcaatcg caaagttata ttgcgaactc
    10921 attgttaaat aaagccgttt acgcgctcta gtaaaagcaa cgtaacatag tcttctttct
    10981 tcctcttcac cagcgcgacc agcatcaagc aacgctcggt tatttggaaa gacaccttca
    11041 acaagtcctg aaacaaaaat ataatcatac tcaagacctt ttgccatatg cacagtcata
    11101 agcgtaatat aatcaccttc ttcaacttca tcttgggcgc tatgtaaact agcgttctct
    11161 aaataatcat taaaggttga ttcaggataa tcattcataa attgtttgat gtcggcaatt
    11221 aagacttgga cgttagcacg catatctgct tgtttatctt catctttttc ggttttaata
    11281 tgttcgtagt aaccaacttt ttgaatatag tcattaataa cagtacttag ttcaagtttg
    11341 tcattttgta attttgttcg tgtctcatcc acagcattca ttaacgcttt taattgttct
    11401 tgtgaagcac ttgataagcc aatatcttca acataatgca ctgtttcaaa tagtgaatat
    11461 tcattcttta acgcagcaat tttaaggttt tccatcgcta cattacccat gccacggcgc
    11521 gggacattaa cgattctgct aaaagaaata tcatcttttt cattaactaa aatccgcaga
    11581 tacgctaaaa catctttaat ctcgcgtctt tgatagaaac gcacgccacc ataaatacgg
    11641 tacggaacat tcttatgtaa aaagtgccgt tcaaatggta gtgttaagta attagcgcgc
    11701 ataagaattg cgacatcacg aaacgaaaaa tctttgacgt ttaagcgcaa tgaaataatt
    11761 tgttcataaa tccaattaac ttcgcgtgaa tcgctttctg tatattgaac aatcactggt
    11821 tcaccttttt ctttagcggt aaaaagattt ttctccactc tatttttgtt atgagcaata
    11881 agtttatttg ctgcatccaa aatatattta gtggaacgat agttttcttc tagaataatc
    11941 gtttcggcat ttttaaaatc actatcaaaa ttcataatga ttcgctcgtt agcgccacgc
    12001 catgtataaa ttgtttgatc gggatcaccc acgacataga gtgatgtatt ttggtgcatt
    12061 aaaagtttaa tgagtttata ttgcaaatca ttagtgtctt gaaattcatc aacgagaata
    12121 taatcaaagc gatgttgcca tttttctcta attgctctat attcctctaa aatcgtaatt
    12181 gatttactaa ttagatcact aaaatcaaga cagtttgacg ctttaagtct tcgttcatat
    12241 tctttccata tttcaacgaa ttctttttca ttaacaaagc cgtgcggtct aattttataa
    12301 gtaaaagcat ctaagccttt ttctttaacc gccccaataa aattatttgc gtcacgcgtt
    12361 aattcgccgc ggcgttcatg tcctctttct acacttatat tgcgaattaa ggaacttgta
    12421 tcttcttcgt catatattgt aaagctgcgt gaatagccta aaacatcaat gtcttcacgc
    12481 agtagtcttg caccaaaaga gtgaaatgtt gaaagacgca cacgaacatt atcgcgttct
    12541 aaatactttt ttaagcgatt tgtcatttcg ccagcggctt tatttgtaaa agtaaaaccg
    12601 acaatgcggt cttccggagt acctatttct tgcactaaat aagcaatacg agtagtgagt
    12661 actcgcgttt taccacttcc cgcaccagca ataacacgca catattgccc gcttgtagta
    12721 acagcttcta actgttttga attaagattt tcatacaaat tcatatctaa aatattataa
    12781 cataatataa ggtgaaatta ctcccgtagt gaagcccttt tttatgttaa aatatgacta
    12841 tgaaaatgaa ctcattatca cgcacacatc aactcgtttt aggcgccctc a
//
LOCUS       DAOSTA010000026        12787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_4928_length_12787_cov_8.993402, whole genome shotgun sequence.
ACCESSION   DAOSTA010000026 DAOSTA010000000
VERSION     DAOSTA010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 12787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12787
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4928_length_12787_cov_8.993402"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..775
                     /gene="deoD"
                     /locus_tag="PLU08_03465"
     CDS             62..775
                     /gene="deoD"
                     /locus_tag="PLU08_03465"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOQ70786.1"
                     /translation="MGTPHINAKKGDFAKTVLMPGDPKRAEWIANTFLTDVKRVTDVR
                     NIFGYTGYYKGKRVSVMASGMGLPSIGIYCHELYTYYGVETIMRVGTCGTYQPEVDLF
                     DIIVAIGSCTDSNWAAQFNLLGGTFSAIGDFDLAVKAKEVANASGINVHVGNILSADI
                     FYDNDPNSWKKWANLGVLGVEMESYALYTTAAKLKKKALCLLTVTDSFIDHSKKATVE
                     ERQTGLEKMIRVALEIAEE"
     gene            778..1437
                     /locus_tag="PLU08_03470"
     CDS             778..1437
                     /locus_tag="PLU08_03470"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HOQ70787.1"
                     /translation="MLFAVFGVKEYVILALAVVLVLFALVNIFLFFRKKILQRFALLR
                     YFYPVVRKTAVYYDYYLINKLEIQLVSNESLFIDHMLFGNKYIYVINDYLFKGVLTGN
                     AQDSKWILKDKKGQEIMVDSPLLKNKKLVEKLSLRTSLDHATFIAITLVSKDTKITDL
                     EVNDSSNFVIDTRDFMKLILKIESRDIAPLNPEDLLKRVHEFDELNLRKKRGRKWRKN
                     R"
     gene            1419..2537
                     /locus_tag="PLU08_03475"
     CDS             1419..2537
                     /locus_tag="PLU08_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70788.1"
                     /translation="MAQKSVTFRTVFKRYKHERRSIKSYLLLTLAFLAFSFVTVYLNS
                     TELALLGAILLVFVIFPLIVVFSEYTALIESGAPAPRNSRAYFDLYKNSYRSGRLRHI
                     FNLRNVVVFLIYFLVGMFIAGLGMTLFIYFFDKPVYDQMFNLMLEFQGTSTPDALNVL
                     VTKIELLLEPYIPSQILAYNLVATLGLIFIIVKGVFNIYLSIFIEHRPTTSFIVLRNT
                     FVGDAETKSSLRRVQTGIVLIVFSLYTLFNVGGYFLLQAINPHGPLFLQAELLGLVVL
                     TIALPFTTRFTFYLYHSVMEKKREQILRFAIVELREIIKNPNLPEQTKTYITQVLKVR
                     EQEYAALTAVEAEQKVVDEKAPASNDDTTNEEIDSDNK"
     gene            2763..5231
                     /locus_tag="PLU08_03480"
     CDS             2763..5231
                     /locus_tag="PLU08_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ70789.1"
                     /translation="MKKKKLLIVGLLGLVALSGCKKPSSSVEAPSEVSSEDVLVETVP
                     AVSGVRTFAGENPAAIKDRKDILTLLESYALDTFIGGIPYRDNSAVVLYSSDLKMPAD
                     DYVIGYGFGVGEAEILAPLTGSQVAPERAKYYHTWTSQDPGTINYMDSQESVTGDLHA
                     LISSGYWGTKFNADKTGYEWYPLLANDVERPIPLNFDEATGTATKWRVPLKVGGDLKY
                     NTLGVHSEFAGLEVKLEDYLVPFKLMVETGWFRGTDLASKDSGFVGVAEELQKPVGER
                     SIERVQGIKLNVAEQAMEFEFNSAKSPFYAMYNLASGLFMPIPQSFIDKIGVENYGKA
                     DIDNTLSLGVYNLEIWEKDKQVVFKKNPLWIEADRYTHEGYVFTILDDSNTAFQQFLA
                     NNLHSAGVPASYLKEYRNDERARQTKGDTTWKLQVNAANQERWNELFGEEGSIAPGGE
                     WELKPVMSNLDFLNGVYYSLNRDHLADTTGSRPAMGFFSEAYMIDPESGVSWRVSEEG
                     QAVLEGRSTETNGFNKEAAGQLFNKAMNDLVAEGKYTRGTAENPTVISPRIHFQSVQQ
                     TVDEGGPLKRMIEAAFNDAVEGFKLDIELFATQNWMDAYYAMMFGEFDFAFGSISGNT
                     LDPLSFMLTLCSDNRSGFTLSWGHDTGHTYNSYEVGGLVFKGERYSYDALLESTLGLT
                     IIEEGKTVPSFSLKGLGFGFNEADPTKYDFVAQGTYYVDPAGEYVITPDPNGTKEETG
                     GFLALVFYNKEGEFQSIVPLPATTFENGAWTVVAEGISVPEDAHYMDIYISTIHTYKG
                     IPEYRANLYELLWSWFAPEAPAGE"
     gene            5363..6397
                     /locus_tag="PLU08_03485"
     CDS             5363..6397
                     /locus_tag="PLU08_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ70790.1"
                     /translation="MGKYILKRVGLVFVTAFIILSLTFILMKLLPMDRPIGLAPDQLR
                     YYNTQVRLGYMVQVQGPLMPGQTVDITVNDGGKLYYYNQVPVMRQYLSWLRNVITRWD
                     WGVSSRIERNVPAIEIIARKLPVSMKLNIIALFVSVPLGFLFGIIAALQKNKLPDHII
                     STGVMIFISIPSFVFITFLMLFFAYKLEWLPSQWPPEGLSWQMQAKAYVIPVMALSFG
                     TIAGFTRYTRAELTEIMSSEFLLLARTKGLTKRQTVIRHALRNSLVPIVPMIIGQFIG
                     ILSGSMILEALYGIPGIGSLFVTAITNKDYNVVMVDMALYTTIGLFAGLLVDLSYGIV
                     DPRIRMGARK"
     gene            6397..8364
                     /locus_tag="PLU08_03490"
     CDS             6397..8364
                     /locus_tag="PLU08_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ70791.1"
                     /translation="MSEKKQLITEKDFVFVQQDEKIHDLKFDTKPTTYFKDAMRRFVK
                     NKSSVVAGVIIGILILLALFVPIFNKNDIVTSQEDYRFLPPRWPGFQNASVLNGRRYV
                     ENIIYDATDEDETKHHPVGYDNEAIVGEVKLTEGTTNFASPYASGGKVLISSDRSSFA
                     TGIQTNKITADFALNDYYLEIVTDTELNRDNNYEIYVLVYYNGPNSDATLVVLQEQSM
                     KYGTFTTNLSDVVLSSRPAAILEDEFTVQIGVYVPATNSPDGTRAQLYLNKLAVTTTA
                     EENPFELHNYTDANELLLRRSDKEKIPYRYQTVISGNQMLYEGRVIRASFEYDYYKAV
                     YGEVNRIIGISDLLNARRDGKVKFDLDPAHSDHTKPDFNYQIVMDTFEVVNDKTSQYR
                     EITFAHPRQTVKAGMTTIYVYNYDVVVSRYREMGYTREPFFLFGTNQRGQDFFKIVFN
                     GLRTSLILGFSAAAINIFIGLVWGAISGYYGGTVDILMERFTEILGGVPWIIIMTLTI
                     LHLGNNFGVFLLALILTGWIGTASVTRSQFYRYKRREYVLASRTLGAKDSRLIFKHIL
                     PNSIGPIVTGAVMMIPGIIFSEATISYLGLGLQGLTSLGVALSEVQAYIYTDPYLIVT
                     GSLIIALLMISFNLFGNGLRDAFNPSLKGVE"
     gene            8364..8576
                     /locus_tag="PLU08_03495"
                     /pseudo
     CDS             8364..8576
                     /locus_tag="PLU08_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            8757..9914
                     /locus_tag="PLU08_03500"
     CDS             8757..9914
                     /locus_tag="PLU08_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002481942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ70792.1"
                     /translation="MYEDLIYEENVAYRNAVTKFRDEIDTSAFKLRNLKVGNKFKAQD
                     IRADKALTKEEKRVKLDALIEEEKAADLEHRALVRRVKAESKPVLTELKAKLKVAQKK
                     AKAEVKAYHKEIKAEYKKERDTLRAELRATKNEERRKELKEALIAAKRNYIDKTRVTK
                     QVAHDMAVKVMEEVGIPNAARRFRQYPFEFSGGMRQRIVIAIALIQNPDILICDEPTT
                     ALDVTIQAQILELILRLKRERQLSVIFITHDLGVVANMADRVAVMYAGRIVEYGKVEE
                     IFYNPKHPYTWALLSSIPDVDTKERLEAIPGTPPNMVYPPKGDAFAPRSKYAMAVDFI
                     EEPPYFKVSETHYARTWLLDPRAPKVEMPKIVSERIRISLERAGKGGEFDA"
     gene            9907..10284
                     /locus_tag="PLU08_03505"
                     /pseudo
     CDS             9907..10284
                     /locus_tag="PLU08_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            10822..11553
                     /locus_tag="PLU08_03510"
                     /pseudo
     CDS             10822..11553
                     /locus_tag="PLU08_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010169069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            11721..12104
                     /locus_tag="PLU08_03515"
     CDS             11721..12104
                     /locus_tag="PLU08_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HOQ70793.1"
                     /translation="MDKTKRNRLIMWLVLSGVTLIAATLILVLRKDYSTRGFSDATFI
                     PGVVVLFLFLLRLVRDAGTFDLVSYSISRIFQGTKEKSLDGMKTASEYVEGKRQERLK
                     KDRYYLPYIIIAVAFIVAGVILAYI"
     gene            complement(12162..12680)
                     /locus_tag="PLU08_03520"
     CDS             complement(12162..12680)
                     /locus_tag="PLU08_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009331709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOQ70794.1"
                     /translation="MNDEKKYVVIVERLARRGVNEMDIAKITYKLQTKYIPDLEMDIC
                     LDHVKRVLKKREVQNAILTGLELDILAEKGMLSEPLQTIIASDYGLYGIDEILALSIV
                     NVYGSIGSTNFGYVDKVKPGIIGELDKKTNGKVVNTFLDDIVGAIAAAAASSIAHKYA
                     DADFDTELYKKQ"
BASE COUNT         4111 a         2327 c         2504 g         3845 t
ORIGIN      
        1 ctcgtaccac gtggagcgca taaatgctat aataaaaaat aaaattaaag gagataatta
       61 tatgggcaca cctcacatta atgcaaaaaa gggagatttt gctaaaactg tattaatgcc
      121 aggcgatcca aagcgggctg aatggattgc aaacactttt cttactgatg ttaaaagagt
      181 aactgatgtt cgtaatattt ttggttatac cggatattat aaaggaaaaa gagtatcagt
      241 tatggcaagt gggatgggtt taccttccat tggcatttat tgtcatgaac tctacacata
      301 ttatggtgtt gaaactatca tgcgtgttgg aacatgcggc acttatcaac cggaagttga
      361 tttatttgat attatcgtcg caattggcag ttgtactgac tctaattggg ctgctcaatt
      421 caatcttttg ggtggaactt ttagtgcgat tggcgatttt gatttagcag ttaaggctaa
      481 agaagttgca aacgcttctg gcattaatgt tcatgtcggc aatattttaa gcgctgacat
      541 attttatgat aatgacccaa attcatggaa aaaatgggcg aaccttggtg tattaggtgt
      601 ggaaatggaa tcatatgcgc tctatacaac cgctgctaaa cttaaaaaga aagcactgtg
      661 ccttttaaca gttactgata gctttattga tcattccaaa aaagcaactg ttgaagagcg
      721 gcaaaccggt ttagaaaaaa tgattcgtgt tgccctagaa attgctgaag aataattatg
      781 ttatttgctg tttttggcgt taaggaatat gtgatattag cgctagcggt tgtgctagtg
      841 ctatttgcac tagtcaatat ttttcttttc ttcagaaaaa agattttaca acgctttgca
      901 ttattgcgat atttttatcc tgtagtgcgt aaaacagcgg tgtattacga ttattatttg
      961 attaataaac tagagattca acttgtttca aatgaatctt tatttattga tcacatgctt
     1021 tttggtaata agtatattta tgtcattaat gattacttat ttaagggtgt tttaactgga
     1081 aacgctcaag attccaagtg gattttaaaa gataaaaagg gacaagaaat tatggttgat
     1141 agtccgcttt taaaaaataa aaaattagtg gaaaaactaa gccttagaac atcgcttgat
     1201 catgccactt ttattgctat tacactcgtg agtaaagata cgaaaatcac tgatttggaa
     1261 gttaatgata gttcaaactt tgtcatagat actcgtgatt ttatgaaact tattttaaaa
     1321 attgagagta gagatattgc cccgcttaat ccagaagatt tgttgaagcg ggttcatgaa
     1381 tttgatgaac taaatctacg caaaaaaaga ggacgaaaat ggcgcaaaaa tcggtaacat
     1441 ttcgtacagt atttaaacgc tataaacacg aacggcgttc tattaaaagc tatttattat
     1501 taacgttagc ctttttagca tttagctttg taacagtata tttaaattcc accgaattag
     1561 cgctacttgg agcgattttg ctagtgttcg ttatctttcc actaattgtt gtattttcag
     1621 aatacacagc acttattgag tctggtgcac cagcgccacg taatagtcga gcgtattttg
     1681 atttgtataa aaactcttac cgcagcggaa gattacgcca tatatttaat ttacgaaatg
     1741 ttgtcgtctt ccttatttac tttttggtcg gaatgtttat agctggatta ggaatgacac
     1801 tcttcattta tttctttgat aagcctgtgt acgatcaaat gtttaactta atgctagagt
     1861 ttcaaggaac atctacacca gacgcattaa atgttttagt aacaaaaata gaattattgc
     1921 tcgaacctta tataccaagt caaatacttg catataacct tgtggcaaca ctcggcctta
     1981 tctttatcat agtaaaaggt gtttttaata tttacctttc gatatttatt gaacatcgtc
     2041 caacaacgag ctttattgtg cttcgcaata cttttgtagg tgacgctgaa actaaaagta
     2101 gtttacgccg tgttcaaaca gggattgttt taattgtctt ttcgctatat actttattta
     2161 atgttggcgg atatttctta ctacaagcca ttaatccgca tggtccgctt ttcttacaag
     2221 ctgaattact tggtttagtt gtgttaacta tcgctttacc atttactacg cgttttacct
     2281 tttatttata tcattcagtt atggagaaaa aacgcgaaca aattcttcgt tttgccattg
     2341 ttgaattacg tgaaattatt aaaaatccaa atcttcccga acaaacaaaa acttacatta
     2401 cacaagtttt aaaagttcgt gaacaagaat acgctgcact tactgccgtg gaagcagaac
     2461 aaaaagtcgt tgatgaaaaa gcgcctgcta gtaatgacga caccactaat gaagaaatcg
     2521 attcagataa taaataaaaa ctgttgactt catcgattta tccaacaatt agtattaatt
     2581 gtccctattt aaacggggct tttattttat aaatattcac taaatttggt aaaaaacact
     2641 tattttttca aatttggaac aagtgtaagc gttatcatga aaatattttc atcctatttc
     2701 ttggcaatct aaatgtacta tgtataataa acctataaaa ctataagaaa ggagaaagtt
     2761 ttatgaaaaa gaaaaaactg ttaatcgttg gacttctcgg attagtcgca ttaagcggtt
     2821 gtaaaaagcc aagctcatct gtagaagcac catcagaagt tagcagcgaa gacgtcttag
     2881 tcgaaactgt tccagccgtt agtggcgtta gaacattcgc tggggaaaat cctgctgcta
     2941 tcaaagatcg taaggatatt ttaacacttc ttgaaagtta cgcccttgat acattcatcg
     3001 gtggtattcc ttatcgtgac aacagtgctg tcgtgcttta ctcaagcgac ctcaaaatgc
     3061 cagcagacga ctatgtcatt ggttatggat tcggggttgg tgaagccgaa attcttgcac
     3121 cacttacagg atcacaagtt gctccagaac gtgcaaagta ctaccatact tggactagtc
     3181 aagatccagg taccattaac tatatggact cacaagaatc cgttactggt gacctccacg
     3241 ctcttatctc ttcaggatat tggggtacaa aatttaatgc ggataagaca ggatatgaat
     3301 ggtatccatt actcgcaaat gacgtcgaac gtccaattcc tttaaacttt gacgaagcca
     3361 ctggtactgc taccaagtgg agagttcctc ttaaagttgg tggagactta aaatataaca
     3421 ctttaggtgt ccacagtgaa tttgctggct tagaagtcaa attagaagac tacttagtcc
     3481 cattcaaact catggttgaa accggatggt tccgcggaac tgacttagcg tcaaaagaca
     3541 gcggattcgt tggtgtcgct gaagaattac aaaaaccagt tggcgaacgt agcattgaac
     3601 gcgtacaagg tattaaactt aatgttgccg aacaagctat ggaatttgaa ttcaattcag
     3661 ccaaatcacc attctatgcg atgtacaact tagcatcagg tttattcatg cctatcccac
     3721 aatcattcat cgacaaaatc ggtgttgaaa actatggtaa ggctgatatc gacaacacct
     3781 tatcacttgg tgtttacaat cttgaaatct gggaaaaaga caaacaagtc gtctttaaga
     3841 aaaacccact ttggattgaa gcagaccgct atacccacga aggttatgtc tttacaatat
     3901 tagatgatag caacactgct ttccaacaat tcttagcaaa caacttacat agcgcgggtg
     3961 ttcctgcaag ttacttaaaa gaataccgta atgatgaacg cgcacgtcaa accaaaggtg
     4021 acactacttg gaaacttcaa gttaacgctg ccaaccaaga acgttggaat gaattatttg
     4081 gtgaagaagg ctcaatcgct ccaggtggcg aatgggaact caaaccagtc atgtccaact
     4141 tagacttctt aaatggtgtc tattattcat taaatcgtga ccatcttgct gataccactg
     4201 gtagccgtcc agcaatgggc ttcttcagtg aagcatacat gattgaccca gaaagtggtg
     4261 tgtcatggcg tgttagtgaa gaaggtcaag ccgtccttga aggccgttca actgaaacaa
     4321 atggctttaa caaagaagct gctggccaac tcttcaacaa agcaatgaac gatttagtcg
     4381 cggaaggtaa atatacacgt ggaaccgctg aaaacccaac cgttattagc ccacgtattc
     4441 acttccaaag tgtccaacaa accgttgacg aaggcggtcc actcaaacgg atgatcgaag
     4501 ctgcctttaa cgacgcagtc gaaggattca aattagacat tgaattattt gcaacccaaa
     4561 actggatgga cgcatactat gcaatgatgt ttggtgaatt tgacttcgcc ttcggaagta
     4621 tcagtggtaa caccttagat ccattatcat tcatgttaac tctttgtagt gacaaccgta
     4681 gtggctttac cctctcatgg ggtcatgaca ctggtcatac atacaattca tacgaagttg
     4741 gtggactcgt cttcaaagga gaacgttatt catatgacgc actcttagaa agtacacttg
     4801 gcttaaccat cattgaagaa ggtaaaaccg ttccatcatt ctcactcaaa ggtttaggct
     4861 ttggcttcaa cgaagctgac ccaaccaaat atgactttgt tgctcaaggt acatattatg
     4921 ttgacccagc tggtgaatat gtcattacac ctgacccgaa tggtactaaa gaagaaactg
     4981 gtggcttctt agccttagtc ttctataaca aagagggtga attccaaagc atcgttccac
     5041 tcccagctac aacatttgaa aatggtgcgt ggactgtagt ggcggaaggc attagcgttc
     5101 cagaagatgc tcactatatg gatatctaca tttcaaccat tcacacctat aaaggtatac
     5161 cagaatatcg tgctaacctt tacgaattac tctggtcatg gtttgcacca gaagcacctg
     5221 ctggtgaata attaattggt acaattatta tcagttaaaa atacaaacta atattagttt
     5281 gctataatta ctaaacatgg acattgccag ccaacgctgg caatgtttct atgtttaatc
     5341 ctatataaga ggaggttaaa ttgtgggaaa atatatacta aaacgtgtcg gtcttgtgtt
     5401 tgtcacagcg ttcattattt taagcttaac gtttatttta atgaaactct taccgatgga
     5461 tcgaccgatt ggattagcac cagaccaact caggtattac aacacccaag tacgccttgg
     5521 ctacatggta caagttcaag gaccattaat gccaggccaa acagtcgata ttactgttaa
     5581 cgacggtggc aaattatatt attacaacca agtgcccgtg atgcgccaat acttaagttg
     5641 gcttcgtaat gtcattacac gctgggactg gggtgtatct tcacggattg aacggaatgt
     5701 gccagcgatt gaaatcatcg cacgtaaact tccagtgtca atgaagttaa atataattgc
     5761 tctctttgtt tcggttccgt taggattttt gtttggtatt attgccgctt tacaaaaaaa
     5821 taaacttccg gaccacatta tctcgactgg agttatgatt tttatctcaa ttccgtcttt
     5881 cgtgtttatt acattcctga tgctattctt tgcttacaaa ttagaatggt tgccatcaca
     5941 atggccgcca gaaggcttat cgtggcaaat gcaagctaaa gcatatgtca ttccagtaat
     6001 ggcgttatcc tttggtacta tcgctggctt tactcgttat acacgtgctg aattaacgga
     6061 aattatgtct agtgaattct tacttttagc aaggacgaaa ggtttaacaa aacgccaaac
     6121 cgttattcgt cacgctttac gtaatagttt agtgccaatt gtgccaatga ttattggtca
     6181 atttattgga attttatcag gatcaatgat tcttgaagca ttatacggaa ttcccgggat
     6241 tggtagctta tttgtaacag caattacaaa taaagactat aacgttgtca tggttgacat
     6301 ggcactttat acaacaattg gtctttttgc cggattacta gtggacttaa gttacggcat
     6361 tgttgaccca agaattagaa tgggggcacg aaaataatga gtgaaaagaa acaattaatt
     6421 actgaaaaag atttcgtgtt tgttcaacaa gacgaaaaga ttcatgactt aaaatttgac
     6481 actaaaccga caacatattt taaagacgca atgaggcgtt ttgttaaaaa caaatcaagt
     6541 gttgtcgctg gtgtgattat tggcatttta attcttttag cgttatttgt gccaatcttt
     6601 aataaaaacg atatcgttac ttcgcaagaa gattatcgct tcttaccacc aaggtggcct
     6661 ggttttcaaa atgcttcagt attaaacggt cgccgctatg ttgaaaacat catttatgat
     6721 gcgactgatg aagatgaaac taaacatcat ccagtaggtt acgataacga agcaattgtt
     6781 ggtgaagtaa aattaaccga gggtaccact aacttcgctt cgccatacgc atcaggcgga
     6841 aaagtgctta tttctagtga tagaagcagt ttcgctactg gtattcaaac gaataaaatt
     6901 actgcagact ttgctcttaa tgattattat ttagagattg taactgatac tgaacttaat
     6961 cgtgataata actacgaaat ttatgtgctt gtgtattata acggtcctaa cagtgatgcg
     7021 acactagtcg tattacaaga acaaagtatg aaatatggta cttttactac taatttaagt
     7081 gatgtagtat tatctagtcg tcctgcagcg atattagaag atgaattcac tgtacaaatt
     7141 ggtgtttatg tgcccgcaac taatagtcca gatggtacgc gcgcgcaatt atatttaaat
     7201 aaattagcgg taacgactac tgctgaagaa aatccttttg aactacataa ctacactgat
     7261 gcgaatgaat tattactacg ccgaagtgat aaagaaaaaa taccatatcg ctatcaaaca
     7321 gttatttctg gtaatcaaat gctctatgaa ggtcgtgtca ttcgcgctag ttttgaatac
     7381 gattactata aagcagttta tggtgaagtt aatcgtatca ttgggattag tgatttacta
     7441 aatgcccgtc gtgatggcaa agttaaattt gaccttgatc cggcccatag cgatcataca
     7501 aaaccagatt ttaactatca aatcgtgatg gatacatttg aagttgtaaa cgacaaaaca
     7561 tcacaatatc gcgaaattac ttttgcccat ccacggcaaa cagttaaagc tgggatgaca
     7621 acaatttatg tatataacta tgatgtcgtt gtttctcgtt atcgggaaat gggttacaca
     7681 cgtgaaccat tcttcctttt tggcacgaat caacgtggtc aagatttctt taagattgtc
     7741 tttaacggtt tacgcacatc cttaatttta ggctttagtg ctgccgctat taatattttt
     7801 atcggtttag tctggggtgc catcagtggt tactacggcg gaacagttga tattttaatg
     7861 gaacgcttta cggaaatctt aggtggggta ccgtggatca ttattatgac cttaacaatc
     7921 ttacacttag ggaataactt tggcgtcttc cttttagcgc ttattctcac aggctggatc
     7981 ggcacggcaa gtgtaacgcg tagtcaattc tatcgttata aacgccgaga atatgtttta
     8041 gcaagtagga cacttggggc gaaagatagt cgcttaattt tcaagcacat cttacctaac
     8101 tccattggtc caattgtgac cggtgccgtc atgatgattc caggtatcat ctttagtgaa
     8161 gcgacgatta gttacttagg gcttgggtta caaggcttaa caagtcttgg ggtcgcgctc
     8221 agtgaagtcc aagcatacat ttatactgat ccatacttaa ttgttaccgg ttcacttatt
     8281 atcgctttac tcatgatttc ctttaacctc tttggtaacg gacttcgtga tgcatttaac
     8341 ccatcattga aaggagtcga ataatgaaac atacagataa agaagtagtc atcaaagttg
     8401 aagacttatc gatttccttt agtactgact atggccggtt gcacgctgtt cgtggggtaa
     8461 gttttgaact ctatcgtggt gaagttctcg ccatcgttgg tgaaagtggc tcaggtaaga
     8521 gcgtatctgc taaaacgatc gtgggcattt tagcggttaa tggtcgtgtt gaaggtggca
     8581 gaataatgta tggtggtgaa gacttaacga aagtcagtga agaagaattc caccgtattc
     8641 gtgggaaaaa gattggaatg attttccaag acccactctc aagcttaaat cctttgatgc
     8701 gtattggtaa acaaattact gaagcgatta ttattaatgg taataagctt aaattaatgt
     8761 atgaagactt gatttatgaa gaaaatgttg cttatcgtaa cgctgtcaca aaattccgtg
     8821 atgaaatcga tactagtgcc tttaaacttc gtaacttaaa agtcggtaat aaatttaaag
     8881 cacaagatat tcgcgctgac aaagcattaa cgaaagaaga aaaacgcgtt aagttagacg
     8941 ctttaattga agaagaaaaa gctgctgatt tagaacatcg tgcgcttgtt agacgcgtga
     9001 aagcagaaag taaaccagtt ttaaccgaac ttaaagctaa actcaaagtt gcgcaaaaga
     9061 aagctaaagc agaagtaaaa gcttatcaca aagaaattaa agctgagtat aaaaaagaac
     9121 gcgatacatt acgcgctgaa ctccgcgcta cgaaaaacga agagcgtcgt aaagaattaa
     9181 aagaggcgtt aattgctgca aaacgtaact atatcgataa aacaagagtg acaaaacaag
     9241 tcgcacacga tatggccgtt aaagttatgg aagaggtcgg tattcctaat gctgcccgac
     9301 gcttccgtca gtacccattt gaatttagtg gtgggatgcg gcaacggatt gttattgcga
     9361 ttgccttaat tcaaaatcct gacatcttaa tttgtgacga gcccacaaca gcgcttgacg
     9421 ttacaattca agcgcaaatc ttagaactta ttttaagact taaacgcgaa cggcaattat
     9481 ccgttatctt tattacgcac gacctcggtg ttgtcgccaa tatggcggac cgcgtcgccg
     9541 tgatgtatgc gggtcgaatt gtcgaatatg gtaaagttga agaaatcttc tacaatccaa
     9601 aacatccgta tacttgggca ttactctcaa gtattcctga tgttgataca aaagaacggc
     9661 ttgaagcaat tcctggtaca ccaccgaaca tggtttaccc acctaagggt gatgcttttg
     9721 ccccacgttc taaatacgca atggcagtcg actttattga agaaccaccg tacttcaaag
     9781 taagtgaaac gcactacgca cggacatggt tacttgatcc acgtgcacca aaagtcgaaa
     9841 tgccaaaaat cgtttccgaa cgaattcgca tttcacttga aagagcgggg aaaggaggag
     9901 agtttgatgc ttaaaaaacg taaaagacgt gaagtaacat ttaaacaaga acttgtcgat
     9961 caagatatta ttcttagtgt acggcattta aaacaattct ttacttcggg acgcggtgct
    10021 aataaattac gcacaaaagc tgttcatgat atttcctttg atattaaaaa aggtgaagtc
    10081 tttggtttag tcggggaatc tggctgtggt aaaacaacaa ccggacgttc gattattaaa
    10141 ctatatgacg tcacaagtgg gagcatttat tttaaaggct accgtattaa cgctggttca
    10201 cgctggaacg aaaaagaaat taaatggaaa acaattcgca ctagacgtga aattaaacgg
    10261 ctgcgtaaag tattacgcgc tgatattaag cgggaaaaag ctgaacttaa acaacaaatt
    10321 aaagatgctg aacttgatat tttagcaaga agttcaccag aactagccgc tgctaagcgt
    10381 gagtttgctg cccttttagt gacaattaga gaacgcaaaa atacatttgc cactgatgaa
    10441 tataactata aaactaaagt tcgtgactta aaacaagcac ttaaagaaga actccgtgac
    10501 cgtagtgacg atagtcaaga tgataatgat attatcgttg cggaaattaa taaaaagtat
    10561 gaaggtcaac ttaaacgtga aacaaataag tttgaaataa ttaaaaaagc ttttgttaag
    10621 gaacatgata aagatagtaa acgtgtttta gaattaaaag cagttattaa agctggtgat
    10681 aaaaatcttt caccaacagt caaagaagaa cttgcggcag agacaaaagt atttgccgtt
    10741 aaagcaaaag aagcttatga cttgcgtgtt agtgaactta aaacagcaac aaacgaagtt
    10801 attgctgact taaaggcaaa acttgcggat atcgtctcga cacaaaaaga aaagattgca
    10861 caaattaact atgatatgaa acatgttgat acaagtatca tgaataagat gcaaatgatt
    10921 ttccaagatc caattgatag tcttaaccct cggatgactg ttgaagatat tattcaagaa
    10981 ggtctccaaa ttcaagggat gaaagaccgc gctgaaaact cgaagaaagt tatcgaatcc
    11041 ttagaaaagg ttggattaat tagcgactat gcttcgcggt acccgcacga atttagtggt
    11101 ggtcaacggc aacggattgg gattgctaga gcgctgatta tgaatcctga attcattatt
    11161 gccgacgaac cagtcagtgc gcttgacgtt tcgattcgcg cacaaattat taacttactt
    11221 aacgacttac gtgatgaact tggcttaacg attctcttta tcgctcacga cttaagtgtt
    11281 gttaaatatt tctgtgaccg gattgcagtt atgtattatg gtaatattgt tgaattaaca
    11341 acgagtgatg agttatttaa acacccatta catccatata caaaagcact cctaagcgcc
    11401 attccaaaac cagatcctta tagtgaaaaa acccgtgaac ggattttata taacccgttt
    11461 accgctcacg attatcgtga agaaaaacca acactacaag aaatcttacc aggtcacttt
    11521 gtcttagcaa atagtgagga aatggaacgt tatcgcgaag aaattaaaga gttagataaa
    11581 aaagcagaag cagaagccaa aaaagaagcg gctagagccg ctaaaaagaa ggcggagcca
    11641 aaggctgaac ccaaaaaagc agcgaagccc aaagcgaaaa gcactgctaa agctaaagcc
    11701 gacaagccga aaaaataacg atggataaaa caaaacgcaa tcgcttaatt atgtggctag
    11761 ttttaagcgg agttacatta attgccgcga ctttaattct tgtgttacgc aaggattatt
    11821 caactcgcgg atttagcgat gcaacgttta ttccaggagt tgttgtctta ttcttatttc
    11881 tcttaagatt agtaagagat gctggcactt ttgacttagt gtcctacagt atctcgcgca
    11941 tcttccaagg tactaaagaa aagtcacttg atggcatgaa aaccgctagt gaatatgttg
    12001 aaggtaaacg ccaagaacga cttaaaaaag atcgttatta cttaccatat atcattatag
    12061 ctgttgcttt tattgtcgca ggagtaatct tagcgtacat ttaacttgga gttaatgaaa
    12121 agcccgattg aaccgggctt ttttcgtgtt ttgttttgtg attattgctt tttataaagt
    12181 tcagtatcaa agtccgcatc agcgtattta tgcgcaatcg aactagcggc cgcagccgca
    12241 atagcgccaa cgatatcatc aaggaatgta ttaacgactt taccatttgt tttcttatct
    12301 aattcaccaa taatgcctgg ctttacttta tcgacataac caaagttagt ggaaccaatt
    12361 gaaccataga cattaacgat tgataacgct aagatttcat caatgccata aagcccataa
    12421 tcgctagcaa taatcgtttg taaaggttca ctaagcatgc ccttctccgc aagtatatca
    12481 agctctaaac cagttaaaat agcgttttgt acttcacgct tttttaatac acgcttaacg
    12541 tgatcgagac atatgtccat ttctaagtct gggatatatt tggtttgtaa tttataagta
    12601 attttagcga tatccatttc attgacgcca cgacgcgcta ggcgttctac aattacaaca
    12661 tattttttct catcgttcat tcgatttctt gtttcgtaat ccatattttt ttacctccta
    12721 ataattatat acatataacg aaataaagtg attcctaatt aaaaaaataa ttagtgattt
    12781 actttat
//
LOCUS       DAOSTA010000027        12612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_5014_length_12612_cov_11.591304, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000027 DAOSTA010000000
VERSION     DAOSTA010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 12612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12612
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5014_length_12612_cov_11.591304"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="PLU08_03525"
     rRNA            <1..145
                     /locus_tag="PLU08_03525"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            239..345
                     /gene="rrf"
                     /locus_tag="PLU08_03530"
     rRNA            239..345
                     /gene="rrf"
                     /locus_tag="PLU08_03530"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            554..904
                     /locus_tag="PLU08_03535"
     CDS             554..904
                     /locus_tag="PLU08_03535"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOQ70795.1"
                     /translation="METKNKTPFGGITISEEAIASAAVDAATSCYGVIGIANQPPKSW
                     LREIFNVDPEPGVVVKKVSGSWHVSLYLNVMYGLKLTEIITSVQDQVKYSLEKTFDIK
                     FKAINIYIVGVEER"
     gene            920..2596
                     /locus_tag="PLU08_03540"
     CDS             920..2596
                     /locus_tag="PLU08_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOQ70796.1"
                     /translation="MKEINGITLKEMFISGANHLYNNYPAIDALNVFPVPDGDTGLNM
                     NLTVSSGAKEISNLNDTNVYRIARAFSKGLLMGARGNSGVITSQIFRGFANGLEGKET
                     MNAVQFGEAMLVGKDVAYKAVMRPVEGTMLTVIREAAEVLNEKAKPDWSIVKALEVMV
                     AEAKASLERTPDLLPVLKEVGVVDSGGAGVVAILEGMLAAITTGIIQKESATATEVVP
                     QYEMAGARITDEEEYGYCTEFILRIGAGEEVKKTFNEERFASVLNSQGNSIVMVRDED
                     ILKVHIHTLRPGTTLTYAQQFGEFVTLKIENMTEQHTHLSDHGKDYFNTNPQVTSASM
                     EMKDYALISVASGEGLSDLFKEYRVDYVVKGGQTMNPSTEDFLAAIKKVHAKTVFILP
                     NNGNIILAATQAAEVTNEEGVVNVHVIPSKTIPQGLVAAMNFNPEVDTEHNIEIMTEA
                     LDAVETGQITFAIKDTSIDGVEVKKDYYMGLQGKKILCSLPDKFDALRELLAGMIDEL
                     TAIVTIIYGEDVEESAAEELLAELEEKYSAVDFELVNGGQPVYSFIVGLE"
     gene            2605..4569
                     /locus_tag="PLU08_03545"
     CDS             2605..4569
                     /locus_tag="PLU08_03545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOQ70797.1"
                     /translation="MAKISNSSRIINALAKYDIHTFRDVIFHLPRRYLDMSYTDEFKM
                     NTNEKVTLKLKVTSAPLLIKPQRVAIIRFTAVSESGATYKIVAYNQRYILNAVKIGDL
                     ITVYGTINLRLLELTVIKLFPRVLSEEEQLSPLYSLPSEVGNHEFIRIVQKALKIESV
                     TSRVPRPLADKNGLISLNDALKRVHNPRDLNDVFEGLKALKYEEGLEYFIKLLLIKRA
                     NSELTNSKKTIVNLKDANEFVKRLPFKLTKDQLTAVREIGYDMNKETLMYRLLQGDVG
                     SGKTVVSFVALYINYLRGAQGVLLAPTESLAKQHYETLLSFFKDFDLRIELLIGALTP
                     SVKTKLKEDIQHGLVDIVVGTHALFSEDVFYPKLELVVIDEQHKFGVNQRNTLLNKGE
                     KADLLLLSATPIPRTLANVIYGDMDISTIESFPHTGRQVNTIITTSASKNVIPLIDKT
                     LSENRQVYIVAPKISFGDKTNVLALYERFSARYPHLVGMLHGELASEEKDAALLNFKS
                     GVTPILVSTTVVEVGIDVKSAGLMIIYEPHSFGLSSLHQLRGRIGRDGKRATLCLVSE
                     EEDNYKLEEFAREDNGFKIAELDLALRGPGELTGEKQSGMPSFMYLNVIKDLKLITII
                     KEDALEVINNPTNPHYFPLLKVINDALEKD"
     gene            4820..5806
                     /gene="plsX"
                     /locus_tag="PLU08_03550"
     CDS             4820..5806
                     /gene="plsX"
                     /locus_tag="PLU08_03550"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOQ70798.1"
                     /translation="MKLVIEMMGGDYGFESTIPALIQFKKDYPEVELFAVGRKEDLAP
                     VADYATIVDARDVVKIDATPLEVLRAKESSLLKAINTVIEEKADALISAGGTGPYLSA
                     ATLKLKLIEGVERAAILAPFPTKIKGKQVAILDIGASNENTASHLYQFALMGQQYARA
                     VFGVEAPKTYLLSNGTEDTKGSPLVLEAFKLYKEKELPYFMGQMEARDALNGEADVVI
                     ADGFTGNVLLKSIEGTAKVLMDSVKGAFKRNLFSKLGYLFARKGFKNMAETFDYKNTG
                     GAILIGVNNVVVKAHGNSDPRGFYSAILVGYKMAKADIVNKVREGLKNVETK"
     gene            5790..6470
                     /gene="rnc"
                     /locus_tag="PLU08_03555"
     CDS             5790..6470
                     /gene="rnc"
                     /locus_tag="PLU08_03555"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOQ70799.1"
                     /translation="MSKLNSLFTKLGITPSNEGLFEEALTHSSYKTKDKDAHDYERLE
                     FLGDAILNMLIASAIYKSHPDKKQGELTNMRKNFVRTQTLATLGRKLELERYIRTGKS
                     ITPQDLYSNDKYFEDVFEALIGAMYLDQGLKVTEDFILRMLGSAIEQYDFNQIFDYIS
                     TLQEFMQADKRGLPQYITEPSGYDNKEEAFISTVTYDGIILGCGYGANSKEAEQNAAK
                     NALEKVAR"
     gene            6472..7452
                     /gene="ftsY"
                     /locus_tag="PLU08_03560"
     CDS             6472..7452
                     /gene="ftsY"
                     /locus_tag="PLU08_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOQ70800.1"
                     /translation="MGLISFLKKKFGGKKNETTDKYVVGLEKSKANFSSKLKRLAKRY
                     EKVTEEYYEHLLEILIGADVGVTLTYEIIEATKEEALLNNLTDPEAINDLLVDKMFIG
                     YLKQGEDIVNEIQFADNGPTVLLVVGVNGSGKTTSIAKLAHRYKKEGKKVLLVAGDTF
                     RAGAVAQLEVWAKRLDVPIVTGKPNADPASVAYEGVAEGKKINADLIIIDTAGRLQTK
                     VNLMAELSKIRRVIGDQIENAPHEVFLIIDATTGQNGVLQAKAFAEATGLTGVVITKM
                     DGTSKGGIILAIRDEIGVPVRFIGLGEKMEDLDEFDLEKYLYGLCLGEDE"
     gene            7452..7730
                     /locus_tag="PLU08_03565"
     CDS             7452..7730
                     /locus_tag="PLU08_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70801.1"
                     /translation="MVVISLLSLVVSFLVFMGVRFAVYSLNPSNYLFADILIIIIGSF
                     IVSLIFAIRQFRYMGRDKWRSQFFHSFVLRTFLINFAIIFIISLLWSW"
     gene            7736..8062
                     /locus_tag="PLU08_03570"
     CDS             7736..8062
                     /locus_tag="PLU08_03570"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70802.1"
                     /translation="MDELKEYMYINELLLIYGKTLTKRQLKIMESYYKYNLGVQEIAE
                     ELNITKAAVSDAIKVSTKHLENLESIVGHYAYRQQVEALHEEIKNTSTLQEVKELLSK
                     EEDDGI"
     gene            8049..9395
                     /gene="ffh"
                     /locus_tag="PLU08_03575"
     CDS             8049..9395
                     /gene="ffh"
                     /locus_tag="PLU08_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOQ70803.1"
                     /translation="MMAFESLTERFSKILKKVRGQSRLTEKNMDEMLQEVRIALFEAD
                     VNFKVVKEFVNNIKEKALGTKVYDTLNPGQMVVKIVNDELVELLGSTDTELIFEKNKP
                     TIIMLVGLQGTGKTTTAGKLALHFKKKMNKRVLLAAADVYRPAAIDQLDTLAKQVGVD
                     IVNLGDKVSPVEIAKAAKKKAYDDRYDVLIIDTAGRLTIDETLMNELKEMKAAVTPTE
                     ILLLVDAMSGQDAVNTALSFNEKLGLTGAIMSKLDSDARGGAALSIKHLTGVPIKFAG
                     VGETLEDLDIFYPDRMADRILGMGDVLTLVEKAQQQLDEKTVKKTTRKIMEGEFDLND
                     MLEQMKQVQKLGKLGGILKLIPGMPKITPEQQELAEREMKLFEVIINSMTPEERSNPA
                     ILRNSRKVRIAKGSGTSNQEINKVIKKYEQMKQMMKQMKGYKKGGPMPPGFPGMGR"
     gene            complement(9680..10036)
                     /locus_tag="PLU08_03580"
     CDS             complement(9680..10036)
                     /locus_tag="PLU08_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70804.1"
                     /translation="MVKTKIYEVSGLCFKGVIPSQMIADKLSVISIDKVFRRIKDLVD
                     LYYLSHVCDFNSEDVLSMLKNNGKELGKFDGFLNRTKDLKHSYEKFRFAGGVSKPTFE
                     EVYLAVKTFIVDVLPQ"
     gene            complement(10093..10380)
                     /locus_tag="PLU08_03585"
     CDS             complement(10093..10380)
                     /locus_tag="PLU08_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70805.1"
                     /translation="MNISNEEKLMYKVMKAIYDSGIPVSFKGSLVLKAFLLESGYTKD
                     TRHTVDIDANWNGKTTPTMEQITESLQKALDKAKINLDVTYFRTIGLLDLN"
     gene            complement(10392..10766)
                     /locus_tag="PLU08_03590"
     CDS             complement(10392..10766)
                     /locus_tag="PLU08_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ70806.1"
                     /translation="MTISEKIKKLRKAQGLTQAELAKGVNVSRTLINKYENGAATPTD
                     GNFISPYAVVSKNGLKYTDLSRTITDAFANEEILDMQGITEAISRYYFTNNEKLDGIA
                     VAPEYQERFERLVSDAIEYHEE"
     gene            complement(10991..12295)
                     /locus_tag="PLU08_03595"
     CDS             complement(10991..12295)
                     /locus_tag="PLU08_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005364223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ70807.1"
                     /translation="MIKRDFYINKLINSKGNGKVKVITGLRRCGKSYLLFKLYKEYLL
                     SQGISEDQIIAYSLEGYENIHLRNINVLYASIKARLKDDNKKYYLFIDEIQNVHPMQN
                     PYVESKDIITFVDLMLEFVKLDNLDIYITGSNSKMLSKDILTQFRGRTHQIRLLPLSF
                     NELYNHFGNTPDLLDNYFMYGGMPEIYTKFRNEEAKRDYLKYLFEETYIKDVLERNNI
                     QNDTFILGTLLDFISSSVGSLISPSKISNRFLSEQNIKISSATVDRYLEYFDDAFVLS
                     KANRYDISGARYYKSNNKYYYTDVGLRNARLNFPLKDQGHIMENIIYIDLIKRGYKVD
                     VGIVHDNYRVDGRLTQKQLEVDFMVTGANERYYIQSVLTLDDLKTTERETRPLNKIKD
                     NFIKIVVIKNYTGSFYDKDGIFYINLKDFLLNENLAEITKRW"
BASE COUNT         4261 a         2030 c         2450 g         3871 t
ORIGIN      
        1 atctaagcgt gaagctaacc ttaagatgag acttcccatt cgcaagaagt aagaccccta
       61 gaaagcgact aggttgatag gtcggaaatg taagtacagc gatgtattca gttgaccgat
      121 actaataggt cgaggactta atatttctat gttaatgaat tgcgccaatt aaatattgct
      181 aaaacgaaag acatgtacat cgaaaaatag tattcagttt tcagagagta atctctggcc
      241 tggtgacgat agcgcagtgg acacacctgt tcccatctcg aacacagaag ttaagcactg
      301 catcggcgat ggtacccgta tggggagagt agcaagttgc caggtttttt tatgccccct
      361 tgcaggtaaa gtagtgatga tacatgaaca atttgactta ctgttcacta aaaaccgtta
      421 tatttttgaa aaaaatataa aaaaatgtta cttaataata ggttattttg ttgagtgaac
      481 gttcatgttg tagtataata tcactacgag gcgaacctca tgtccttttt cgaaagcctc
      541 gtaaacaaat attatggaaa caaaaaacaa aactccgttt ggaggaatta ctattagtga
      601 agaagcaatc gcgagcgcag ctgttgacgc ggcgacgtcg tgttatggcg taattgggat
      661 tgcaaatcaa ccgccgaagt catggcttcg cgagattttt aatgttgatc cagagcctgg
      721 tgtcgttgtt aaaaaagtaa gtggcagctg gcatgtttcc ttatatttaa atgtaatgta
      781 cggattaaaa ttaacagaaa ttattacatc ggtacaagac caagtcaaat actcattaga
      841 gaagactttt gacatcaaat ttaaagcgat taatatctat attgtcggcg ttgaggaacg
      901 ctaaagagga gtttgtggaa tgaaagaaat taatggaatt acactgaagg aaatgttcat
      961 ctccggggca aaccacttat ataacaatta cccggcgatt gatgctttaa acgttttccc
     1021 tgtgcctgat ggtgacacag gacttaatat gaatttaact gtctcaagtg gggcaaaaga
     1081 aatatcaaac ttaaatgata cgaatgtcta tcgtatcgcc cgcgccttta gtaaagggct
     1141 cttaatgggg gcacgcggta atagtggtgt gattactagc caaattttcc gcgggttcgc
     1201 taatgggctt gaaggtaaag aaacaatgaa cgctgtccaa tttggtgaag caatgttagt
     1261 cggtaaagat gtcgcttaca aagcggttat gcgtcctgtt gaaggcacaa tgctcaccgt
     1321 tattcgtgaa gcggctgaag tgttaaacga aaaagctaag cctgattggt caattgttaa
     1381 agcgcttgaa gtaatggttg cggaagctaa agcaagttta gagcgtaccc ctgatttatt
     1441 accagtttta aaagaagttg gcgtcgtcga tagtggtggc gctggggttg tggccatttt
     1501 agaaggaatg cttgcggcca ttactaccgg tattattcaa aaagaaagtg caaccgcaac
     1561 ggaagttgta ccgcaatatg aaatggcggg agcgcggatc actgatgaag aggaatacgg
     1621 ttactgtact gaatttatct tacgaattgg cgctggtgaa gaagtaaaga aaacatttaa
     1681 tgaagagcgt tttgcaagcg tcttaaattc acaaggtaac tcaattgtga tggtacgcga
     1741 cgaagatatc ttaaaagtgc acattcatac tttaagacca gggacaactt taacttatgc
     1801 gcaacaattt ggggaatttg tcacgcttaa aattgaaaat atgacggaac aacatacgca
     1861 cttaagtgat cacggcaaag attattttaa cactaatccg caagtgacta gtgcaagcat
     1921 ggaaatgaaa gactacgctt taatttctgt cgctagtggt gaaggattaa gtgatttatt
     1981 taaagaatat cgtgtagatt atgtagttaa gggcggacaa acaatgaatc cgtcaaccga
     2041 agacttttta gcagcaatca aaaaggtgca tgctaagact gttttcatct tacctaataa
     2101 cggtaacatc attttagcgg caacccaagc tgctgaagtc actaatgaag aaggtgttgt
     2161 taatgttcac gttattccaa gtaaaacaat cccgcaaggc ttagtcgcag cgatgaactt
     2221 taaccctgaa gttgatactg aacataacat tgaaataatg actgaagcac ttgacgctgt
     2281 tgaaactgga caaattacct ttgcaattaa agatacatca attgatggtg ttgaagttaa
     2341 aaaagattat tatatgggtt tacaaggtaa aaagattctc tgttccttac ctgataaatt
     2401 tgatgccctt cgcgaactcc ttgctggcat gattgatgaa ttaacagcaa ttgtgacgat
     2461 aatttacggt gaagatgttg aagagagcgc cgcagaagaa ttacttgcgg aacttgaaga
     2521 aaaatattcg gcagttgact ttgaattagt aaatggcggc caacctgtat attcgtttat
     2581 cgtcggtctc gagtagaggt agcgatggcg aaaataagta attcatcacg aattattaat
     2641 gcattagcaa aatatgacat ccacacattt agggatgtca tttttcattt accgcgtcgt
     2701 tatttagata tgtcgtacac tgatgaattt aagatgaaca cgaatgaaaa agtgacactt
     2761 aaattaaaag taacgagtgc accactttta attaaaccgc aaagagtggc gattattcgt
     2821 ttcaccgctg ttagtgaaag tggcgcaact tataaaattg ttgcctacaa ccaacgctat
     2881 atattaaacg ccgtcaaaat aggtgactta atcacggtat acggcacaat taatttgcga
     2941 cttttagaac taacagttat taaactcttt ccgcgggtgc taagcgaaga agaacagctg
     3001 agtccactct atagtttacc aagtgaagta ggcaatcatg agtttataag aatcgtgcaa
     3061 aaagcactta aaatagaaag tgtaacttcg cgtgttccgc gccctttagc cgacaaaaac
     3121 ggtttaattt cgctaaatga tgccttaaaa cgtgtgcata atccacgtga cttaaatgat
     3181 gtctttgaag gacttaaagc acttaaatac gaagaaggat tagaatattt tattaagctt
     3241 ttacttatta aaagggcaaa tagcgaatta actaattcaa agaaaacaat tgttaattta
     3301 aaagacgcaa atgaatttgt taaaagacta ccatttaagt taacgaaaga tcaactaact
     3361 gccgtgcgag aaattggtta cgatatgaat aaagaaacac tgatgtatcg tttgcttcag
     3421 ggtgatgttg ggagcggtaa aacagttgtg agttttgtcg cactctatat taattacttg
     3481 cgtggagccc aaggtgtctt acttgcgcca acggaaagtt tagcgaaaca gcattatgaa
     3541 acgctccttt cattctttaa ggattttgat ttacgaatcg aattactaat tggggcttta
     3601 acgccaagtg ttaaaaccaa attaaaagaa gatatccaac atgggttagt cgatattgtc
     3661 gttggtaccc acgctttatt tagtgaagat gtcttttatc ctaaacttga actcgtggtc
     3721 attgatgaac aacataaatt tggcgttaac cagcggaata cactattaaa caaaggcgaa
     3781 aaggctgatt tactcttact tagcgcgaca ccgataccgc gcacacttgc aaatgtaatt
     3841 tatggcgata tggatatttc aacaattgaa agttttccgc atactgggcg acaagtgaat
     3901 accattatta cgacaagtgc gtctaaaaat gtgataccgc ttattgacaa aacactctcg
     3961 gaaaatagac aagtttatat agtcgcaccg aagattagtt tcggtgataa aacaaatgtt
     4021 ttagcgcttt acgagcgttt ttcagcgcgc tacccacatt tagtgggtat gcttcacggc
     4081 gagcttgcaa gcgaagaaaa agacgcggcg ctcttaaact ttaaaagtgg tgtaacacca
     4141 atcttagtgt caacgacagt cgttgaagtc gggattgatg tgaagagtgc gggacttatg
     4201 ataatttacg aaccacatag ttttggactt agttcattgc atcaacttcg aggcagaatt
     4261 ggccgcgacg ggaaaagggc aacactttgt ttagtgagtg aggaagaaga taattataaa
     4321 cttgaagagt ttgcaagaga agataacggc ttcaaaatag cagaacttga tttagcactc
     4381 cgcgggcctg gtgaattaac gggagagaag caaagcggga tgcctagctt tatgtatctt
     4441 aatgttatta aggatttaaa actaattact atcattaaag aagatgcgtt ggaagttatt
     4501 aataacccga caaatccgca ttattttccg cttttaaaag taattaatga tgctttagaa
     4561 aaagactaat ataaatatct gtctaatatt tttgcaggaa catacacttt taatcaatag
     4621 gaattatagt tactttttca ttgttttaag tcacaaaata cgaaattgtc gtctcattat
     4681 aaaggttatt tttaatttga ctttcaagat ttaaaaaatt gaaagagttg aaagtgcaga
     4741 gctttaaacg gcttagttta tataaaaagg tgattatggt aaaataactt tggttatttt
     4801 taataaggag ataacgacta tgaaacttgt aattgaaatg atgggtggcg attacggatt
     4861 tgaaagcaca atcccagcgc ttatccagtt taaaaaagat tatccagaag ttgaactttt
     4921 tgctgtgggt cgcaaagaag atttagcgcc agttgcggat tacgcaacga ttgttgatgc
     4981 gcgtgatgtt gttaaaattg atgcgacacc gcttgaagtg ttacgcgcaa aagaaagttc
     5041 gcttttaaaa gcaattaata cggtaataga agaaaaggct gatgcgctta ttagtgcagg
     5101 cggaacaggc ccatatttaa gtgcggcaac gcttaaactt aaattaattg aaggtgttga
     5161 acgggccgcc attttagcgc ccttcccaac gaaaataaaa ggcaaacaag tcgcaattct
     5221 tgatattggg gcaagtaacg aaaatactgc gagtcactta tatcaatttg ccctaatggg
     5281 acaacaatac gctagagcgg tatttggagt agaagcacca aaaacttatt tattaagtaa
     5341 cggaactgaa gacaccaaag gttcaccttt agtgcttgaa gcttttaaac tttataaaga
     5401 aaaagaatta ccttacttta tgggtcaaat ggaagcacga gacgcgttaa atggcgaggc
     5461 tgatgttgtg atagcagacg gctttactgg aaatgtgtta cttaaatcaa ttgaaggaac
     5521 cgcaaaagtc ttaatggaca gtgtaaaagg cgcttttaaa cgaaacttat tttctaaatt
     5581 aggatatctc tttgcaagaa aaggtttcaa aaacatggca gaaacctttg attataaaaa
     5641 tacaggtgga gcgattttaa ttggtgttaa taatgttgtc gttaaagcac atggtaatag
     5701 tgatccacgc ggcttttata gcgctattct tgttggttac aaaatggcca aagctgatat
     5761 tgtgaataaa gtacgtgaag ggttaaaaaa tgtcgaaact aaatagtctt tttactaaat
     5821 tagggattac gccaagtaat gagggactct ttgaagaagc gttaacgcat tctagttata
     5881 aaacaaaaga caaagacgcg catgactatg aaaggcttga gtttttaggt gacgctattt
     5941 taaatatgct gattgctagt gcgatttata aaagtcatcc tgataaaaaa caaggtgaat
     6001 taacgaatat gcggaaaaac tttgtgcgca cacaaacgct agctacttta ggacgcaaat
     6061 tagaattaga acgctatatt agaactggta aatcaattac accgcaagac ttatatagta
     6121 acgataaata ttttgaagac gtttttgagg cattaatcgg tgcgatgtat cttgatcaag
     6181 ggctaaaagt aacggaagac tttattttaa gaatgttagg cagtgcgatt gagcaatatg
     6241 actttaatca aatttttgat tacattagta cgctacaaga attcatgcag gctgataaaa
     6301 ggggattacc acaatacatc actgaacctt cgggttacga taataaagaa gaagccttta
     6361 tttcaacagt gacatatgac gggattattc ttgggtgtgg ctacggcgca aatagtaaag
     6421 aagccgaaca aaatgcagca aagaatgctt tagaaaaggt ggcgcggtaa gatgggatta
     6481 atttcatttt taaaaaagaa gtttggtggg aagaaaaacg aaaccaccga taaatatgtt
     6541 gtcggtctag aaaaaagtaa ggcgaacttt tctagtaaac taaaacgctt agcaaaacgc
     6601 tatgaaaaag taacggaaga atattatgaa cacttacttg aaatcttaat tggcgcagat
     6661 gttggtgtta ctttaactta tgaaattatt gaagcaacaa aagaagaagc actgcttaat
     6721 aatttaactg accctgaagc tattaatgac cttttagtcg ataagatgtt tattggttac
     6781 ttaaagcagg gggaagacat agttaacgaa attcagtttg cagacaatgg tccaaccgtg
     6841 ttacttgtgg ttggtgttaa cggcagcggg aaaacaacat caattgctaa actagcgcat
     6901 cgctataaaa aagaaggaaa aaaagtttta ttagttgcag gcgatacctt ccgcgctggt
     6961 gcggttgcac aacttgaagt atgggcaaag cgccttgatg tcccgattgt gacaggaaaa
     7021 ccaaacgctg atcccgcaag tgtcgcttat gagggagtcg cagaagggaa gaaaattaac
     7081 gctgatttaa ttattattga tactgcgggt cgcttacaaa ctaaggtgaa cttaatggca
     7141 gaattaagca agattcgccg ggttattggt gaccaaatag aaaatgctcc gcatgaagta
     7201 tttttgatta ttgatgcgac aactggacaa aacggagtgc tgcaagctaa agcatttgcc
     7261 gaagcaactg ggcttactgg tgttgttatt acaaagatgg atggcactag taagggcgga
     7321 ataattttag cgattagaga tgaaattggt gtgccagtac gctttattgg cttaggcgaa
     7381 aaaatggaag acttagatga atttgattta gaaaaatatt tatatggctt atgcctaggg
     7441 gaggacgaat aatggttgtt atttccttac ttagtttagt agtgtctttt cttgtgttta
     7501 tgggagtgcg ctttgcggta tattcgctta atccttcaaa ctatttgttc gcggatattt
     7561 taattattat cattggtagt tttattgtta gtcttatctt tgccattcgc caatttagat
     7621 atatgggaag agataaatgg cgctcacaat tctttcacag ttttgtgctt cgtacattct
     7681 taattaattt tgcgattatc tttataattt cgttactctg gtcttggtga taaaaatgga
     7741 tgaattaaaa gagtatatgt atattaatga gttattacta atttatggta aaacactcac
     7801 aaaacgacaa ctaaagatta tggaaagcta ttacaaatat aatcttggtg tccaagaaat
     7861 agcagaagaa ttaaacatca ctaaagcagc ggtgagcgat gcgattaaag tatccactaa
     7921 acacttagaa aatttagaaa gtattgtggg gcattacgct tatcgccaac aagtggaagc
     7981 actgcacgaa gaaataaaaa acacttcaac attacaagaa gttaaagaac tcttaagtaa
     8041 ggaggaagat gatggcattt gaatcattaa ccgaacgatt tagtaaaata ttaaaaaaag
     8101 tccgcggtca atcgcgtctt accgaaaaaa atatggacga gatgctccaa gaagtgcgga
     8161 ttgccctttt tgaagcggat gttaatttta aagttgtaaa agaattcgtt aataatatta
     8221 aagaaaaagc actaggcacg aaagtttatg acacacttaa ccctggtcaa atggtcgtaa
     8281 aaattgttaa cgatgaatta gtggaacttt tagggagcac tgatactgaa ctaatttttg
     8341 aaaaaaataa accaacaatt attatgcttg ttggtcttca aggaactggt aaaacaacga
     8401 ccgctggtaa attagcgctg cattttaaaa agaaaatgaa taaaagagtg ttacttgctg
     8461 cagcagatgt ctatcgacca gcggcaattg atcaacttga tacactagcg aaacaagtcg
     8521 gtgttgatat tgttaattta ggtgataaag ttagtcctgt tgaaattgcg aaagccgcga
     8581 agaaaaaagc atatgacgat cgatacgatg tcttaattat tgacactgcg ggacgcttaa
     8641 caattgatga aacattaatg aatgaattaa aagagatgaa agcagcagtg acaccaacgg
     8701 aaattttatt acttgttgac gccatgagcg gacaagacgc ggtaaatact gctttatcct
     8761 ttaatgagaa gttagggctt acgggcgcta ttatgtctaa acttgatagc gacgctcgtg
     8821 gtggggcggc tttatcaatt aaacatttaa ctggtgtacc aattaaattc gctggtgttg
     8881 gtgaaacgct agaagactta gatattttct atcctgaccg gatggcggac cgtatccttg
     8941 ggatggggga cgttttaacg ctcgttgaaa aagcacagca acaacttgat gaaaagacag
     9001 tgaaaaaaac aacgcgcaag attatggaag gcgagtttga tttaaatgat atgctcgaac
     9061 aaatgaaaca agtacaaaaa ttaggaaaac tcggggggat tttaaaatta atcccaggca
     9121 tgcctaaaat tactcctgaa caacaagaac ttgccgaacg cgaaatgaag ctctttgaag
     9181 ttattattaa ctcaatgacg ccagaagaaa gaagtaaccc cgctattttg cggaatagtc
     9241 gtaaagtaag aatcgctaaa ggcagtggca cgagtaatca agagattaat aaagtgatta
     9301 agaaatatga acaaatgaaa caaatgatga aacaaatgaa gggatataaa aaaggcggac
     9361 caatgccacc gggattccct ggaatgggaa ggtaaaaaaa gaacacaaat actgacacca
     9421 atagaggtga ttgaagttaa cgttcaatta gaatatgcag aagaacttat cgtggaaaaa
     9481 gatacattta cgattaaaat ttagttttaa caatggaaat ttgtgaaaaa atgaataatt
     9541 gtatttaatg atgtaatggc ctttgctgtc attaacgcat cctttacaat atgtgcaata
     9601 tttttttgct gaaacaaaaa gtcttgattt aatttattaa tagttaaatc ctttgattag
     9661 ttcataatac tagcgattgt tattgtggga ggacatctac aataaaagtc tttactgcca
     9721 aatatacttc ttcaaatgtc ggcttactca ctccacctgc aaaacgaaac ttttcgtaag
     9781 aatgttttaa atcttttgtt cggtttagaa atccatcaaa tttacctaac tctttcccgt
     9841 tatttttgag catacttaaa acatcttcac tattaaaatc acaaacatga gaaagataat
     9901 ataaatccac aagatcttta attctccgaa agaccttgtc tatagaaata acagataatt
     9961 tatccgcgat catttgcgac gggataacgc ctttaaaaca tagtcctgaa acctcataaa
    10021 tctttgtctt aaccattaga cgattaacat ctatgtccat ggtaaaaagt agttctcgag
    10081 atgacctgtc tcttaattca aatccagcag acctattgtc ctaaaataag taacatcgag
    10141 gttaatcttt gctttatcaa gcgctttttg taaggattca gtaatttgtt ccattgtagg
    10201 tgtagttttt ccattccaat ttgcgtctat atcgacagtg tgacgagtat ctttggtata
    10261 tccagactca agtagaaatg ctttgagcac caacgagcct ttgaaactta ctgggatacc
    10321 gctgtcgtat atagctttca taactttata cattaatttt tcttcatttg aaatgttcat
    10381 tactttatcc tctattcttc gtgatattca attgcgtcac taacgagcct ttcaaacctt
    10441 tcttgatatt ctggagcaac agcaatccca tcaagtttct cgttatttgt gaagtaatag
    10501 cgactaatag cctcagtaat accttgcata tctaaaatct cttcattagc gaaagcatca
    10561 gtaatagttc tacttaaatc tgtgtacttt aaaccatttt tacttactac agcataagga
    10621 cttataaagt taccatcggt aggagtagcg gcaccatttt catatttatt tataagtgtt
    10681 cgcgaaacat ttactccctt agcaagctca gcttgggtaa gaccttgcgc ttttcttaat
    10741 tttttgattt tttctgaaat tgtcactatg cttctctctt aataaaaatc actttactag
    10801 attttaaata aatattcatg tgaaactata gtgaaaaaag tgaaagatga ttgctagatg
    10861 taatagtcct tttatctaaa cgctattttc tgaatgatta gaagtgtaga tacctacatt
    10921 ttagaccgcc tttttcctac aaatttatta tgataattac attaacaaca ctatcgtttc
    10981 attataatga ttaccatctt ttagttattt ccgctaaatt ttcatttaat agaaaatctt
    11041 ttaaattaat ataaaatatt ccatccttat cataaaatga accagtgtag tttttgataa
    11101 ctactatttt aataaagtta tcttttattt tattgagtgg tcttgtttct ctttctgttg
    11161 tttttaaatc atctaaagtt agaacagatt ggatatagta tcgttcattt gcccctgtta
    11221 ccataaaatc aacttccaac tgtttttgtg taagtctccc atctactcga taattatcat
    11281 gtactattcc aacatctact ttataccctc tttttattaa atctatatat attatatttt
    11341 ccatgatgtg tccttgatct tttagtggaa aattaagcct tgcatttctt aacccgacat
    11401 cagtgtaata gtatttgtta ttgctcttat aataccttgc tccacttatg tcgtatcgat
    11461 tagcttttga taatacaaat gcatcatcaa aatattctaa atatcgatcg actgtagcgc
    11521 tagatatttt tatattttgc tcagataaaa atcgattaga aattttagaa ggactaatta
    11581 aagaaccaac gctagacgat ataaaatcga gaagtgtgcc taatataaat gtatcatttt
    11641 gaatattgtt tctttctaaa acgtctttga tataagtttc ctcaaaaagg tatttaagat
    11701 aatctctttt agcttcttca ttacgaaact tagtgtatat ttctggcata ccaccataca
    11761 taaaataatt atctaataaa tccggagtgt ttccaaaatg gttatatagt tcattaaaag
    11821 atagtggaag taatcttatt tgatgggttc tgcctctaaa ttgggttaat atatctttag
    11881 ataacatttt agagttcgaa ccagtaatat atatatctaa attatctaat tttacaaact
    11941 ctaacataag gtcaacaaaa gttattatgt ctttagattc aacataagga ttttgcattg
    12001 gatgaacgtt ttgaatctca tcaatgaaaa gataatattt tttattatca tcttttagtc
    12061 ttgctttaat gctcgcatat aaaacattta tattccttaa atgtatattt tcataccctt
    12121 ccaaactata tgcaataatt tgatcttcac ttataccttg gcttaacaaa tattctttat
    12181 atagtttaaa taatagataa gatttaccac atcttcgtaa accagttatc actttaactt
    12241 ttccgttgcc tttactattt attaacttat taatatagaa atctcttttt atcatattac
    12301 taactcctaa aataaagtca taatgttgac tgtttttcac gtgtatttta acattgacgt
    12361 caaaaatagt caacaagttg attgttttta acatcatttc gatacaagaa aagcaagaat
    12421 cctttttagt accttcgctg ctaattgctc aattaattta attttatata ataattacat
    12481 agtgattcca cttcgtttag cagcttaaaa aatggtaatt ttagttcaat tatcgcgaaa
    12541 aaccatattt tcagtctttt cataattaaa aaatgcatta ataattgtat caaaacagac
    12601 taagctagat gg
//
LOCUS       DAOSTA010000028        12604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_5021_length_12604_cov_13.748267, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000028 DAOSTA010000000
VERSION     DAOSTA010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 12604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12604
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5021_length_12604_cov_13.748267"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1904)
                     /gene="ppdK"
                     /locus_tag="PLU08_03600"
     CDS             complement(<1..1904)
                     /gene="ppdK"
                     /locus_tag="PLU08_03600"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOQ70808.1"
                     /translation="MAEKRYVFAFKCKEGEKLGRNYLGGKGANLAEMTRIGVPIPQGF
                     TVTTEACNLYYNEGKKLPQFVIDQIYNAVTELEKETGKTFGGKKNPLLVSVRSGARVS
                     MPGMMDTVLNLGLNDEVAEVLANETGNAAFAFDSYRRFILMYFNVVKGQPRAEMEKML
                     EDMKVKAGVKLDSELKAEHLRELVKQFKAHYKKVLGEEFPSDARVQLIEAVTAVFRSW
                     DNERANIYRRMNHIPYEWGTAVNVQSMVYGNKGDTSGTGVAFTRSPSTGDNHIFAEYL
                     PNAQGEDVVAGIRTPLPISWLEENMPAVYKQFVETLDLLEKHYRDMQDIEFTVEDGKL
                     YFLQTRSGKRTAAAALKIVCDLIDEGLITEEEAVLRVDPKALDSLLHKGFDQAVLKTK
                     KVIATGLPASPGAATGRLSFDAADAAARKEKGETVLLVRSETSPEDIAGMVSAAGILT
                     SRGGMTSHAAVVARGMGTTCIVGAHDVIIDEVGHTMKVGDKVYTTKDVISIDGTTGNI
                     YEGEIPLVEPQLTGYFGRFMELADKYRRLEVRVNADTPLDATHGRDFGATGIGLCRTE
                     HMFFDVDRVFFMRKMILSATKEEREAALAQILPYQENDFYEIFKVMKGLPVTVRLLDP
                     PLHEFLPTEEA"
     gene            2072..3613
                     /locus_tag="PLU08_03605"
     CDS             2072..3613
                     /locus_tag="PLU08_03605"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOQ70809.1"
                     /translation="MEKKEKLVLAVDFGTQSLRVSIVNQFGEIKAIIKNKYNEAYFSS
                     EPCFAEQNPNYYWYELCNATYKLKEKHDDLLQKVLAMSVTTFRDSPVFLDKNYEPTRP
                     MVLWLDQRQAALHKKPPFLRRLIFAIVGMSETVTLNMKRTPAIWVQENEKDVWEKTAY
                     YVNFSTYINYKLIGELIDSVANQTGHFPIHFKKGKWYGDNALKNIYRVPRTKLAKIVK
                     QGTVMGVISEAASKATGLPQGLKIIAAGTDKGSEAFGTGAYRKDIASISYGTASSISV
                     PNKKYIEPELFLPAYSAPIDGLYQMEVQIYRGYWMIGWFIKQFGTHETLEAKIETMAT
                     EEVLNNKMLEIPPGSQGLVLQPYWGPGLKRPEARGAIVGFSDYHTRIHVYRAIIEGIA
                     FGLKEGLLGIEKRQHKKVKEIRVSGGGSQSDAICQITADIFGVPVSRTQTYETATIGT
                     ALVTFYYLGVFDSLETATKAMVHIEKTFIPNPKNVKQYNKLFKDVYAKLYPKLKGIYK
                     KLKYF"
     gene            complement(3671..5506)
                     /locus_tag="PLU08_03610"
     CDS             complement(3671..5506)
                     /locus_tag="PLU08_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ70810.1"
                     /translation="MARHGRGRKMEKARNSKLAWRGLLREFKPYRWRIIIIAVLLTIA
                     NVANTLIPLLLRNILGAFSPENVRYGRYFLDVTNLALGLDWTKVFIDFGLMIGVIVAA
                     ALLGTIAQLIAIKISGAYAYNLRNKIRAKIDRLPLSYFDKHPHGELLSIGTNDVDAIA
                     QTLNNTLITVVSSITLFVAILIGMLLVNWRLALVALGMLPFVVIITLVITKNSQNQFI
                     TFQKKTGELEGVVEENFSGLQVIQLFNQQDKQLETFTNLNDSMAKSNFWSQWLSSFIF
                     PSIRFINNLGFVGVLLVAGVINDVISLAPFIFFLNNFMQPFLNLGQISANIQTTLAGA
                     ERVFNLLNAAEQEKEPEDALTTNDHVVGEFCFKDVDFSYNPDVELIKNMNLHVKPGDT
                     IAIVGPTGAGKTTLVNLIMRFYEINGGSITLDGTDIRNYSRSALRRSVGMVLQDTWLF
                     KGSIKNNIRYGNRDATDEEVIEAAKAARAHHFITTFPGEYEFILNEDGTNISQGQRQL
                     ITIARAIISKPKILILDEATSSVDTRTEYAIQSVMNQIMKDRTTFIIAHRLSTIKNAK
                     KILVMNKGNIIETGTHEELLAANGFYAGLYNAQFLGIDDSQETVD"
     gene            complement(5506..7329)
                     /locus_tag="PLU08_03615"
     CDS             complement(5506..7329)
                     /locus_tag="PLU08_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ70811.1"
                     /translation="MFRLYKLVKRYWWQLAIIVVLVATQTLLQLQLPDYITTTQNIVN
                     NIGETGITRAEINEMLKQSGIMLLISLGIFALAAGQGSLNSKFVATLGKEIRAEVFKK
                     VENFSLSDVNEFGTASLLTRLINDSRAVQEFAFMVNRVFIMSPIFLIIGSVKLLTLDP
                     MYFYVVIVIIPLIIIVLIITFAVASPLFTQIQERVDYVTLLLREGLTGVRVIRAFNQE
                     ERENRFFDEGNRKMTDIIIKVSNVMQIISPIINILFNLVFFGIYLIGFVLFDKMLLGV
                     IPFNMNAFSQTITAMMYAQHIMMSFMMLSFTFIMFPRVKVSAKRINAVLNVKPNIVDP
                     EAPLDKELIDTGIVKFENVTFTFSDLEHPTLKNINFTAEPGTTTAIVGSTGSGKSSII
                     NLLPRFFDASVGKVTFDGYDVRDFKLNDLRDKIGFVPQTAVLFKGTIRDNLRFGNENA
                     TDEEMWEALRVAQAAGFVSKLELGLDAPVAQGGKNFSGGQRQRLAIARALVRKPKIYV
                     FDDSFSALDFKTDIRLRSALKDYAKDATIFVVAQRVNSILDAHQILVLDNGEVAGIGK
                     HADLLNSCKVYQEIVTSQLDPEEIKKSRDMFKDFTTEGGKA"
     gene            complement(7330..7938)
                     /locus_tag="PLU08_03620"
     CDS             complement(7330..7938)
                     /locus_tag="PLU08_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ70812.1"
                     /translation="MARRTTSRTKSTTKTRRDDILEAGIRLFARVPFSELTIAAVARE
                     ANCGHSLVYHYFTNINRLYDECVNHVVARYKPFIEHFKSLTIPAELALAGSIALIVEM
                     LKREKLAPYCLSLLLYTYKQAPRNKAILTVREDWSKLFLNLISEGQKNERIISTLSAN
                     EILNVLKSILRGLISDQIVSGPSPANILTASEIYLPIMQGIN"
     assembly_gap    7996..8095
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8096..8774)
                     /locus_tag="PLU08_03625"
     CDS             complement(<8096..8774)
                     /locus_tag="PLU08_03625"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOQ70813.1"
                     /translation="MRLDRFLAKTLGETRTTVKKYIRTKKVTVNGEVIISDGFQVDTI
                     NDVVAFDGEVLQYEEFQYYLINKPEGYVCANEDNVNPTIISFAPEFMMYKLHTVGRLD
                     KDTTGALLLTNNGKLTHQLISPKKNVEKVYLATTDLPIKGELVAHFARGIKIDEDFTT
                     LPATLEIVESNVGRVTVVEGKFHQIKRMFQAFGLNVVKLHREQFAGLRVDDLEIGEYR
                     KLEHDEIN"
     gene            complement(8771..9445)
                     /locus_tag="PLU08_03630"
     CDS             complement(8771..9445)
                     /locus_tag="PLU08_03630"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOQ70814.1"
                     /translation="MEWEITFLRWVVENTQTPFITAIFKFLTLSGELAVLYFIIAFIM
                     IGFKKTRKAGLIIVFGLILVSGFNLWIFKPIVNRPRPFDVPEFSADALWLNNYMYNVF
                     APQSKLNDFLVPGSKAFMSGHTLSSFIFGFTILFHHRKWSVPALILSVIMSYTRIYFG
                     FHYPTDVFAGIITALATVLLFTWLANKHETWFINLWRKFISLFKKKSKVDDTTAIETE
                     HQEEAQ"
     gene            complement(9470..10399)
                     /locus_tag="PLU08_03635"
     CDS             complement(9470..10399)
                     /locus_tag="PLU08_03635"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOQ70815.1"
                     /translation="MNRFLKRFLQGLLLGFALITAIGGGTMAVLIGVYDELINAVSDF
                     RKDPKKSLSLLGPMALGGIVSMGALFYPIKLALEHFPFLSISFFAGLTLGGLRIFTGT
                     IKGNVTWKNLLLTLAGFLFVFGLGTFTWFTTLHADLVNINFLQVILLFFVAFLSMGGH
                     VSAGISGTFILIAFGYYEELIDFLIRLAKFDFVNVGFDLAGLLSFGIGSILGLIVIAK
                     TYKYFFEKDRVKTYFTIFGFIIGSIVIAFFNGKVRPEYAKVTGFDPLMFTLCVVTVIV
                     GAALSYFLLGLANKKEKQQTVEEVAINSNNKED"
     gene            10446..11027
                     /gene="pth"
                     /locus_tag="PLU08_03640"
     CDS             10446..11027
                     /gene="pth"
                     /locus_tag="PLU08_03640"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006506439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOQ70816.1"
                     /translation="MKLFVGLGNPGKKYENTRHNAGFLALDKFAEIAKVDIDKSDFKG
                     LFTSFTYNAEKIYLLKPQTYMNLSGQSVVSLMNFFKIEVDELYVIYDDMDISPGQIRL
                     KEDGSSGGHKGMQNIIELLGTTNIKRIRIGIGKPEHDGVDHVLQKLTDEDKKIFMEGV
                     TKASVAVRDLLNEDFNTVMNKNNRKEPKPPPSD"
     gene            11020..>12604
                     /locus_tag="PLU08_03645"
     CDS             11020..>12604
                     /locus_tag="PLU08_03645"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOQ70817.1"
                     /translation="MIKLFETLFPKNTPPRFEKKRVFVTSEIIGTTLLVAEAFKKKRE
                     NYVVLTNNLYNAQKIISTLQTFVSQDDILFFPNDDLLFTEVLTATKELEAQRIYTLFS
                     SLEKDKKYIYVANQSALLKSTLSPTSFAKQFFELKVGNTVKMSALTTKLVELGYKNVT
                     NVEQTMQFAKRGDIIDIYTVNEDFPTRIEFFDDEIESIRDFNVSTQVSIKTRDKVTML
                     PASENLLFPNEQKEFLKCLQEFFNVQKKDMTSAEEEMFATNLNASISSYLEGDNNALN
                     KYLLIYSLFQNSLLSYIDNPKVVFANEPQLLATYEIISNHAEHYVRDQARRFNLLPKT
                     QLYQPYNTLDHYSKYVTREFSEDGNEGELRISPVADLRSRDDFNLVVQKYYDDNYLIY
                     FSVNNNTQLEQVKNLLKELQLDFDLDDKSSDKRILITYDYFPHGFVINSCKTAVFTSK
                     ELFNITRNEMRFNARFKAGTVIRSHEQLTPGDYVVHEYHGIGQFMEIESLKVFGVVSD
                     YIKIKYFGNEELSIPMEQIH"
BASE COUNT         4309 a         2275 c         2152 g         3768 t
ORIGIN      
        1 gcttcttcgg ttggtaagaa ttcgtgtaac ggtgggtcaa gtaaacgaac agtgactggt
       61 aatcccttca tcaccttgaa gatttcatag aagtcatttt cttggtatgg aaggatttgc
      121 gctaaggctg cttcacgttc ttcttttgta gcggaaagaa tcattttacg cataaagaat
      181 acacgatcaa catcaaagaa catatgttcg gtacggcata agccgatacc agttgcacca
      241 aagtcgcgac catgggtggc gtcaagtggt gtatcagcgt taacacggac ttcaagacga
      301 cgatatttgt cggctaattc catgaaacga ccaaagtaac cagtaagttg tggttcgact
      361 agtgggattt caccttcata gatgttccct gtagtaccat caatactaat tacatcttta
      421 gtagtgtaaa ctttgtcacc aactttcatt gtgtggccga cttcatcaat gatgacatcg
      481 tgcgcaccaa cgatacatgt tgtacccatt ccgcgcgcaa caacagcagc gtgactagtc
      541 ataccaccgc gtgaggtgag aataccagca gcggaaacca taccagcaat gtcttctggt
      601 gaagtttcac tacgaactaa gaggacagtt tcgccttttt ctttacgggc agcagcatca
      661 gcggcatcaa agcttaaacg gccggtggca gcgcctggag aagctggtaa accagtagcg
      721 ataactttct ttgtttttaa gactgcttga tcaaagcctt tgtgaagtaa actgtcaagt
      781 gcttttggat caacgcgtaa gacggcttct tcttcagtaa ttaagccttc atcaattaag
      841 tcacacacga ttttaagtgc agcagcggcg gttcttttac cactgcgtgt ttgtaagaag
      901 tataatttac catcttcaac agtaaattcg atatcttgca tatcgcgata gtgtttttct
      961 aataaatcta aagtttctac aaattgtttg tagacggcag gcatgttttc ttctaaccaa
     1021 ctaattggta gcggtgtacg aataccagca acaacgtctt caccttgtgc atttggtaag
     1081 tattccgcga aaatgtgatt gtcaccagtg gatggtgaac gggtaaaggc aacaccggtt
     1141 ccacttgtat cacccttgtt accataaacc attgattgca cgtttacagc agtaccccat
     1201 tcgtaaggaa tgtggttcat tctacgatag atatttgctc tttcgttatc ccagctacgg
     1261 aagactgcgg taacagcttc aattaattgg acacgagcgt cacttgggaa ttcttcacca
     1321 agaacttttt tataatgagc tttaaattgt ttaactaatt ctcttaaatg ttcagccttt
     1381 aattcactat caagcttaac accagcttta actttcatgt cttcaagcat tttttccatt
     1441 tcggcacgtg gttggccttt aacaacgttg aaatacatta aaataaaacg acgatagctg
     1501 tcaaacgcaa atgctgcgtt accagtttca tttgctaaaa cttcggcaac ttcatcattg
     1561 agtcctaagt ttaaaacagt gtccatcatc ccaggcattg atacacgagc accagaacga
     1621 acacttacga gtagtggatt tttcttacca ccaaatgttt taccagtttc tttttctaat
     1681 tctgtaactg cattgtaaat ttgatcaatc acaaattgtg gtaatttttt accttcattg
     1741 taataaaggt tgcaagcttc agttgtgacg gtgaaccctt gtggaatagg aacgccaatg
     1801 cgtgtcattt cagcaaggtt agcaccttta ccgcccaagt aattccggcc taatttttcg
     1861 ccttctttac acttgaacgc aaagacatat cttttttctg ccatattttt cctcctttta
     1921 ataagcagta tttaacatac catcgctaat tataatatca tagatattaa ggtttattac
     1981 aataaatcac tcaaaaacag cgaaaattga agagaaaaca ctataatagt aataatttgc
     2041 taaaatagta atgctaaagg aaaaatcgaa tatggaaaag aaagaaaaac ttgtactagc
     2101 agtcgacttt ggtacccaat ctctaagagt ctcgattgta aaccaatttg gagaaatcaa
     2161 agcgatcatt aaaaataaat ataacgaggc ttattttagt agtgagcctt gttttgctga
     2221 gcaaaatcca aactactatt ggtatgaatt atgtaacgcc acatataaat taaaagaaaa
     2281 gcatgatgac cttttacaga aagtactagc gatgagtgtc acgactttcc gtgatagccc
     2341 agtatttctt gataaaaact atgaaccaac tagaccaatg gtgttgtggc ttgatcaacg
     2401 tcaagcagcg ctccataaga aaccaccatt tttaagacgc ttaattttcg ctattgttgg
     2461 gatgagtgaa acggtcactt taaatatgaa acgcacacca gcgatatggg tgcaagaaaa
     2521 tgaaaaagat gtttgggaaa aaacagcgta ttatgttaat ttctctactt atattaacta
     2581 taaattaatt ggtgaactta ttgatagcgt cgctaatcaa acaggtcact tcccaatcca
     2641 ttttaaaaaa gggaaatggt atggtgataa tgcattaaag aatatttatc gtgttccgcg
     2701 cactaaatta gcgaaaattg taaaacaagg aacggtaatg ggtgttataa gcgaagccgc
     2761 aagtaaagca acaggacttc cgcaaggatt aaaaatcatt gcagcgggaa ctgataaagg
     2821 aagcgaagca tttggaacag gtgcgtaccg caaagatatt gcttcaattt cctatggcac
     2881 tgcaagttca atatctgttc cgaataaaaa atacattgaa cccgaattat ttttaccagc
     2941 ctatagcgca ccaattgatg gtctttatca aatggaagtg caaatatatc gtggctactg
     3001 gatgattggc tggtttatta aacaatttgg tacgcatgaa acacttgaag ccaaaattga
     3061 aacaatggcc actgaagaag tgttaaataa taaaatgctt gaaataccac caggctcaca
     3121 aggactcgtc ttacaaccat attggggtcc aggacttaaa cgcccggaag ctcgtggtgc
     3181 gattgttgga tttagtgatt atcacacacg cattcatgtt tatcgggcga ttattgaagg
     3241 gattgctttt ggtttaaaag aagggttact cggtattgaa aaaagacaac ataaaaaagt
     3301 aaaagaaata agagtatcag gaggcggttc gcaaagtgat gcgatatgtc aaattaccgc
     3361 tgatatattt ggtgtcccag ttagtcggac acaaacttat gaaaccgcaa caattggtac
     3421 agcgcttgtt actttctatt atttaggtgt gtttgattca cttgaaacag ccacaaaagc
     3481 gatggtccac attgaaaaaa catttatacc aaatcctaaa aatgttaaac agtataataa
     3541 attatttaaa gatgtatacg ctaaacttta tccaaaatta aaaggcatat ataagaaatt
     3601 aaaatacttc tagaagaaaa aataaaaggg ttaatcgaca aattaaccct tttttgctgt
     3661 aaataattga ttaatccaca gtttcttggc tgtcatcaat accaaggaat tgtgcattat
     3721 aaagaccagc gtaaaatccg tttgctgcta aaagttcctc gtgagttccc gtttcaataa
     3781 tgttaccttt attcattacc aaaatcttct tagcattttt aatggtgctt aagcggtggg
     3841 caataataaa tgtagtgcgg tctttcataa tttgattcat aacactttga atcgcgtatt
     3901 ccgtacgcgt atcaacacta ctagtagctt catcaagaat aaggatttta ggtttagaaa
     3961 taattgcacg agcaatcgta attaactggc gttgcccttg cgaaatatta gtgccatctt
     4021 cattaagtat aaactcgtat tcacccggaa aagtagttat aaagtggtga gcgcgcgctg
     4081 ctttagcagc ttcgataact tcttcgtcag tagcgtcacg attaccataa cgaatattat
     4141 ttttaatcga acctttgaag agccaagtat cttgtaaaac catcccaaca ctacggcgta
     4201 atgcacttcg tgaataatta cgaatatcag taccatctaa tgtaattgaa ccaccattta
     4261 tttcatagaa tcgcataatt aaattaacta gtgttgtttt gcccgcgcca gttggaccaa
     4321 caatagcgat tgtatcccct ggtttaacat gtaaattcat atttttaata agttcaacat
     4381 cagggttata actaaaatca acatctttga agcagaattc ccccacaaca tgatcgtttg
     4441 tagttaatgc atcctctggt tctttttctt gttcagcggc attaagtaaa ttaaacactc
     4501 tttctgcacc agcaagtgtt gtttgaatat ttgcagatat ttgccctaag ttaaggaatg
     4561 gttgcataaa gttatttaag aaaaaaataa atggcgcgag actaataaca tcatttataa
     4621 cacccgctac aagtaaaaca ccaacgaagc caagattatt aataaagcga attgatggga
     4681 aaataaatga actcagccat tgtgaccaga aattagattt agccatagaa tcattaagat
     4741 ttgtaaaagt ttctagttgt ttatcttgtt gattaaatag ttgaatgact tggagtccgg
     4801 aaaagttttc ttccactacc ccttctaatt cacctgtttt cttttggaaa gtaataaact
     4861 gattttgtga atttttcgta ataacaagcg taataattac tacaaacgga agcatgccaa
     4921 gtgctactaa ggcaagccgc caatttacaa gtaacatgcc aattaaaatt gcaacaaaaa
     4981 gagtgattga tgaaacaaca gtgataagcg tgttatttaa tgtttgggca atagcatcaa
     5041 catcatttgt gccaattgaa agtaattcac catgcggatg tttatcaaaa taagaaagtg
     5101 gtaaacgatc aattttcgcg cgtattttat tacgaagatt ataagcgtac gctccagaaa
     5161 ttttgatagc aatcagttgt gcgatagtac caagtaatgc agcggcaaca ataacaccaa
     5221 tcattaatcc aaaatcaatg aagacctttg tccaatctaa ccctaacgct agattagtaa
     5281 catctaaaaa ataacgacca taacgaacat tttcaggcga gaaagcaccc agaatattac
     5341 gcaataatag cgggataagc gtattagcta catttgcgat agtaagaagc acagcaataa
     5401 ttattatccg ccaacgataa ggtttaaatt cgcgcaaaag accacgccaa gctaattttg
     5461 aattgcgcgc tttttccatt ttgcgtccgc gtccgtggcg tgccattatg ctttaccccc
     5521 ttcagttgta aagtctttaa acatatcacg cgacttttta atttcttcag gatctagttg
     5581 tgatgtaacg atttcttgat ataccttaca cgaattaagt aagtcagcat gtttgccaat
     5641 accagcaacc tcgccattat caaggactaa gatttgatgg gcatcaagaa tagaattaac
     5701 acgctgtgct acaacaaaaa tagttgcgtc ttttgcgtaa tcttttaatg cgcttcttag
     5761 tctaatatca gtcttaaagt ctagtgcact aaaagaatca tcaaacacat aaattttagg
     5821 ttttctaaca agtgcacgcg ctatcgctag gcgttgtctt tgaccaccac tgaaattttt
     5881 gccaccttgt gcaactggtg catcaagtcc aagttcgagt ttagaaacaa aacccgcagc
     5941 ttgcgccacg cgtaaagctt cccacatttc ttcatcagta gcgttttcat taccgaagcg
     6001 taaattatca cgaattgtac ctttaaataa cactgcagtt tgtggaacga agcctatttt
     6061 gtcgcgtaaa tcatttaatt taaagtcacg cacatcatag ccgtcaaaag ttactttacc
     6121 aacactagca tcaaaaaatc gtggtaataa attaatgatt gaactcttgc cagaaccagt
     6181 agaaccaaca attgctgttg tagttcctgg ctctgctgta aaattaatat tctttaatgt
     6241 tgggtgttca agatcgctga aagtaaaagt tacattttca aacttaacaa taccagtatc
     6301 aattaattct ttatcaagcg gtgcctccgg atcaacaata tttggtttaa catttagcac
     6361 agcgttaatt cgctttgcgc taactttaac gcgcgggaac ataataaagg taaagctaag
     6421 catcatgaaa ctcatcataa tatgttgagc gtacatcatc gctgttattg tttgactaaa
     6481 agcattcata ttaaaaggaa taacaccaag gagcatttta tcgaacaaga caaaaccaat
     6541 aagatatatt ccaaaaaaca ctaagttaaa taagatatta ataatcggac ttataatttg
     6601 cataacatta ctaactttaa taataatgtc agtcatttta cgattgcctt cgtcaaagaa
     6661 gcggttttca cgttcttctt gattaaaggc acgaatgaca cgaacaccag ttagtccttc
     6721 gcgtaataat agcgtcacat aatccactcg ttcttgaatt tgggtgaaaa gcgggcttgc
     6781 tacggcaaaa gtaataatca atacaattat aatcaaagga ataataacga taacgacata
     6841 gaaatacatt ggatctaatg ttagtaattt aacagaacca ataattaaaa agattggtga
     6901 cataataaaa acacgattta ccataaaagc aaattcttgc acagcgcgtg aatcgttaat
     6961 tagcctagtt aaaagactag cggttccaaa ttcattaaca tcacttaatg aaaagttttc
     7021 aacttttttg aacacttccg cgcgaatttc tttaccaagg gtagcaacaa atttagagtt
     7081 taatgagcct tggcctgctg ctaaagcaaa aataccaagg gaaattaaaa gcataatacc
     7141 gctttgtttt aacatttcat ttatttcagc gcgagttatc ccagtttcac caatattatt
     7201 aacaatgttt tgagttgttg taatataatc aggtaattga agttgaagca atgtctgagt
     7261 agcgacgagg acaacaataa tcgctaattg ccaccaataa cgtttaacta atttataaag
     7321 tctaaacatt tagtttatcc cctgcataat tggtaagtaa atttcagacg ctgttaagat
     7381 attagcgggc gacggaccag aaacaatttg gtcactaatt aaaccgcgta atattgattt
     7441 taaaacattt aatatctcat tagcgcttaa tgtactgatt atacgctcgt ttttttggcc
     7501 ttcactaatg agatttaaaa aaagttttga ccaatcttcc cgcactgtta atattgcttt
     7561 attccgtggt gcttgtttgt aagtatatag caacagactt aaacaataag gagctagttt
     7621 ctcccttttt aacatttcta caatcaaagc aattgaaccc gcgagcgcta gttcagcggg
     7681 aattgttaat gatttaaagt gttcaataaa aggcttataa cgagcaacaa catgattgac
     7741 acattcatca tataagcgat taatatttgt gaaataatga taaacaagac tatgaccaca
     7801 attagcttcg cgcgcaacag cggcaattgt taattcagaa aaagggacac gagcaaaaag
     7861 tcgtattcct gcttcgagaa tatcgtcgcg tcgagttttt gtggtacttt tagttctact
     7921 tgttgttcgt cttgccatag ttataccatt gcaaaaatta taacaaagat gaacctaaat
     7981 agatattgaa ttaatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaatta
     8101 atttcgtcat gttctaattt tcgatattca ccaatttcta aatcatcaac acgcagtcct
     8161 gcaaattgtt cgcgatgaag ttttacaaca tttaacccaa acgcttgaaa catccgtttt
     8221 atttgatgaa atttaccttc cacaacagta actctaccta cattagattc cactatttct
     8281 aaagttgcag gaagtgtcgt aaaatcttca tcaattttaa ttccacgcgc aaaatgcgcg
     8341 actaattcac ctttaatagg taaatcggtg gtagcaagat aaaccttttc aacattcttt
     8401 tttggactta taagttggtg tgtaagttta ccgttgtttg ttaatagtaa ggcgcctgtc
     8461 gtatccttat ctaaacgacc aacagtatgt aatttataca tcataaattc tggcgcaaaa
     8521 gaaataattg tagggttaac attatcttcg tttgcacaca catatccttc tggcttatta
     8581 attaaataat attgaaattc ttcatattgt aaaacttcac catcaaaagc gacgacatca
     8641 ttaatagtgt ccacttgaaa accgtctgaa ataataactt caccattgac agtcactttt
     8701 ttagtgcgaa tgtatttttt aacagtggtt ctagtttcac cgagagtttt tgctagaaaa
     8761 cgatctaatc tcattgtgct tcttcttgat gctcagtttc aattgcggta gtatcgtcta
     8821 ctttagactt tttcttgaag agactaataa atttacgcca aagattgata aaccaagttt
     8881 cgtgtttatt tgctaaccaa gtgaaaagaa ggacagtggc aagagctgtt atgatacccg
     8941 caaagacatc agtaggataa tggaatccaa aataaatccg ggtgtatgac attattacac
     9001 ttaaaattaa agcgggcact gaccatttcc gatgatggaa taaaatagta aatccaaaaa
     9061 taaaagatga aagcgtatgt ccagacataa atgcttttga accaggcact aaaaagtcat
     9121 taagcttact ttgtggcgca aagacgttat acatatagtt gtttaaccat aaagcatccg
     9181 cagaaaattc aggaacatca aaaggtcgcg gccgattaac aatcggttta aaaatccaaa
     9241 gattaaaacc agatacaaga attaaaccaa aaacaatgat taatcctgct tttctcgtct
     9301 ttttaaatcc aatcataata aaagcaatta taaaatagag aactgcaagt tcgccagaga
     9361 gtgttaaaaa cttaaaaata gcggtaataa acggtgtttg tgtattttct acaacccagc
     9421 gtaaaaaagt aatttcccat tccatagtta agtactcctt ttttatattc taatcttctt
     9481 tattattact atttatagca acttcttcaa ctgtttgttg tttttctttt ttatttgcta
     9541 aacctaaaag gaagtaagaa agagccgcgc ctacaataac agtaacaaca caaagagtaa
     9601 acattaaggg gtcaaaacca gtaactttag cgtactctgg ccgtacctta ccgttaaaga
     9661 aagcaataac aattgagcca ataataaaac caaagatcgt gaaataagtt ttaacgcggt
     9721 ctttttcaaa gaaatattta tatgtcttag caataacaat taaccctaaa attgaaccga
     9781 taccaaacga taagagtccc gctaagtcaa aaccaacatt cacaaaatca aatttagcaa
     9841 gtctaattaa gaaatcaatt aattcttcat aatatccaaa agcaatgaga ataaatgtac
     9901 ctgatatacc tgcagaaaca tgaccgccca ttgaaagaaa ggccacaaag aaaagcaaga
     9961 tgacttgtaa gaaattaata tttactaaat cagcgtgtaa agtcgtaaac caagtaaagg
    10021 ttcctaagcc aaatacaaaa aggaaacccg caagtgttaa aagtaagttt ttccaagtga
    10081 cattgccttt aatagtacca gtaaatattc gtaaaccgcc gagtgttaaa ccagcaaaaa
    10141 atgaaattga aagaaaagga aagtgttcaa gcgctagttt gattggataa aaaagcgcgc
    10201 ccatacttac aataccgcca agcgccattg gtccgagtaa actaagcgac ttttttggat
    10261 ctttcctaaa atcagaaaca gcattaatta actcatcata aaccccaatt aaaacagcca
    10321 tagtcccgcc gccaatcgcg gtaattaagg caaatcctaa gagcaaacct tgtaaaaaac
    10381 gttttaagaa tctattcata tcataaataa tagcacaata aatgttataa tttatcagta
    10441 aaaatatgaa actatttgtc ggtttaggta atccaggaaa gaaatatgaa aacacgcgcc
    10501 acaacgctgg gtttttagcg cttgataaat ttgccgaaat cgctaaggtt gatattgata
    10561 aaagtgattt caaagggctt ttcacgagtt ttacttacaa cgctgaaaaa atatatttat
    10621 taaaaccaca aacatatatg aatctttctg gacaatcagt agtgtcacta atgaacttct
    10681 ttaaaattga agttgatgaa ctttatgtaa tttatgatga tatggatatt tctccaggac
    10741 aaatccgttt aaaggaagac gggtcatctg gcggacacaa aggaatgcaa aatattattg
    10801 aactcttagg aaccacaaat attaaacgga ttcgtattgg cattggtaaa ccagaacacg
    10861 acggagtgga tcatgtttta caaaaactta ctgatgaaga taaaaagatt tttatggaag
    10921 gcgtcactaa agcaagcgtt gccgttcgtg atttgttaaa tgaagatttc aatactgtaa
    10981 tgaataaaaa caaccgaaag gagccaaaac ccccaccgag tgattaaact ttttgaaaca
    11041 ttgtttccta aaaatacacc ccctcgcttt gaaaaaaagc gagtttttgt cacaagcgaa
    11101 atcataggga caacgctatt agtcgccgag gcttttaaga aaaagcgaga aaactatgtt
    11161 gttttaacga acaatttata taatgcacaa aagataattt caaccttaca aacatttgtt
    11221 agtcaagacg atattttatt tttcccaaat gacgatttat tatttacgga agtattgacc
    11281 gcaactaaag aactagaagc acaaagaatt tacacattat tttcatcact tgaaaaagat
    11341 aaaaaatata tttatgttgc taaccaatca gcgctgctta aatcaacgct ttctccaaca
    11401 tcttttgcca aacaattttt cgaactaaaa gtaggaaata ctgttaaaat gtcagcgtta
    11461 accaccaaat tggtggaatt aggatataaa aatgtcacta atgtcgaaca gacgatgcag
    11521 tttgcaaaac gcggtgatat tatcgatata tatacagtaa acgaagactt tccgacgcgt
    11581 attgaattct tcgatgatga gattgaatca atccgtgact ttaatgtaag cacacaagta
    11641 tcaattaaaa ctcgtgataa agtaactatg cttcccgcaa gtgaaaactt attattcccc
    11701 aatgaacaaa aagaatttct taagtgcttg caagaatttt tcaatgtaca gaaaaaagat
    11761 atgactagcg cggaagaaga aatgtttgct actaacttga atgcaagtat tagtagttat
    11821 ttagaaggtg ataataacgc tttaaataaa tatctattaa tttattcatt gtttcaaaat
    11881 agtttgctct cttatattga taatccaaaa gttgtattcg ctaatgaacc acaattactt
    11941 gctacatatg agattatttc taatcacgca gagcattatg ttagagacca agcaagacga
    12001 tttaacttgt taccaaagac acaactttat caaccttata acactttaga ccactattca
    12061 aaatatgtaa cacgagaatt tagcgaggac ggcaatgaag gtgaactccg tataagtccg
    12121 gtagcggatc ttcgaagtcg tgatgatttt aatttagtag tgcaaaaata ttatgatgat
    12181 aattatctta tttatttctc agttaacaat aacacacaac tagagcaagt taaaaattta
    12241 ttaaaagaat tacaattaga ttttgattta gatgataaaa gtagcgataa aagaatatta
    12301 attacctatg actattttcc gcatggattt gtcattaatt cctgcaaaac agctgtattt
    12361 actagtaaag agctatttaa tattacgcgt aatgagatgc ggtttaatgc caggtttaaa
    12421 gccggaactg tgattcgtag tcatgaacaa cttaccccgg gtgattatgt agttcatgaa
    12481 tatcacggta ttggtcaatt tatggaaatt gagtccttaa aagtatttgg tgttgttagt
    12541 gactatatta aaattaaata ttttggcaat gaagaattat caattccaat ggaacaaatt
    12601 catt
//
LOCUS       DAOSTA010000029        12226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_5201_length_12226_cov_8.152247, whole genome shotgun sequence.
ACCESSION   DAOSTA010000029 DAOSTA010000000
VERSION     DAOSTA010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5201_length_12226_cov_8.152247"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..441
                     /locus_tag="PLU08_03650"
     CDS             112..441
                     /locus_tag="PLU08_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70818.1"
                     /translation="MLRFWLYVFAYWYTAAIVLVLFLAFALIGLTIFERYPFYHDLLA
                     ERSKGEEKRSLTIAFLVFIMAINIFWRIYGEAYKYIILAGVLGWGIGDALAALDGKQK
                     ISIVIET"
     gene            442..684
                     /locus_tag="PLU08_03655"
     CDS             442..684
                     /locus_tag="PLU08_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70819.1"
                     /translation="MLSTKKTIQGIFAMFLGSTIVIFPVLFLVGSKDWIYSLITALVA
                     GIIGTFVELYTKDGWDTLTLPSALMVILYVASIIVQ"
     gene            818..3550
                     /locus_tag="PLU08_03660"
     CDS             818..3550
                     /locus_tag="PLU08_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70820.1"
                     /translation="MNNIISEHITVEKPTLVKFWQKYKAKIFSASVTTLIVVLLFLFS
                     GNAYVEFAISKRLEALLLLSLLGISGILFLLNISFVKVKNYVLSLKSNLKNKKIILTL
                     VFSAVFIGLAAATLIINIVSNRGNFTGILRMISVYATIVMLVVTYKKERIIDTFLLSV
                     RILIIASLAVFILSLFLPSHYVTEIYVTKANTLVYNYFHLSFILRSWGYISAGIPRFS
                     SIFWEPSIAAGILSIALFVRIFYKKHSMFDTFLYVIAVVFTLSTGGIFLFGLLFIYYI
                     MTVLDKREKIVFGSIILVGSIFAFIFRESIFSALATLSPQLFGKLSTQSTPFLTRLNS
                     LPTFIKIFGEKPIFGHGPFGAITRYYEVIAGSSLDAATSTFGFLIAAFGSMGVFLVAL
                     IITAPLFLQEKWTNRIFASLFFIVLLNLQNVPFLALFMLFPVIFILEGVFNKKTSLTE
                     NNPVNERVYAYFFMSKTEGSAVVKNINGALIIKGLSMIVGIIAIPVYRTFFISEEYYG
                     LWMAIISILTWILFFDFGFGIGLRAKLGPLLDAGEEKDAQEKISSTYVGSIFIALGLL
                     VVFAVLIWSLDLNGILNTPTSLVSEMQLKISMSILAAGFCIEFVLKNVLFVLYSMRKS
                     AIASFVTFLSMLSLLLFFIIFKDFNITNRLLVSSIVYVVCINVPLIVASFVLYARKKA
                     LLPTFKGFSLASMKSVMSIGLVFFLIQIGFLFITQTDGLLITSMFSTNANAQYTYYSR
                     IFVFFVGLMGAVVQQPIWSAISAAREQGDTKMINKYIKVTFGIALFLFVICLFIGVGL
                     PFIFELWLGDHAPTVNSFFVIVFLLYSFIFLFSNALIIVANGLTILKSQAVITLIVAA
                     LKIPVLIGLMYLLPQLEWSVVVLVTTLCYVPYLIFLPIYIHKMIKKVRHEEKIA"
     gene            3531..4640
                     /locus_tag="PLU08_03665"
     CDS             3531..4640
                     /locus_tag="PLU08_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70821.1"
                     /translation="MKKKSLKYILFMLSALIAPALLSVGVALADVNFNYFVMSNAAKY
                     IKEGEEKMLVRLDNKDEINEDWRSFATTYNRRHQTYSSIFRHYSDVVVSTAGSERNVH
                     LADINLRSSNVVDTIEGIKIIDMGHSFQDLLTADHSLVISTLYATTLMNEKGVSEMKD
                     LLNVEVNVTRDNLTIVHKVRAIYDVNVKDEYANNKYYETFGDLFFVIRENTPLFDNPE
                     YYLTLGRDQDHNITIYDDLQYLKTKWAANVAKTSIDSEAQTLINKYDELIKDASFNLL
                     RASFIIVGALVTIFNIVVITLKVKQGKKDWALKKFDGWTIALLIVMGAPLLLMLVTSN
                     IPLFGLILNRLTPLGIALYVLSALSVYLAYKALCT"
     gene            4680..5888
                     /locus_tag="PLU08_03670"
     CDS             4680..5888
                     /locus_tag="PLU08_03670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOQ70822.1"
                     /translation="MKKTCVIVSTMYCPTGSASGLRISSVVDVLITLGYDCHVIGLSD
                     YDVFKTHEVKPGYTVHSIYNIKRKNNAFEKLKVFLYPKKRFKTIIEKIKQERKIDLLI
                     IYQQLPLQSSRYILNVAKKEKIKLLYDFVEWQVLGQQNLVSYFTFYRPNQKYIKKYSK
                     YGLNIAISTFLNEYFLSQNIDSLYVPFFFDVKSKVYVEDNKPFKPNQDKIYLGYFGKP
                     FGGRDTIINAIKGILLLPKEKQAKFEFIMAGPNDNDLIKLGLTPAELEAAQPFLKVLG
                     RIPHEQLVDIYKYIDYTILLKPAEKRFSKAGFPTKVSESLSYAVPSICNLTGDLELFL
                     EHEKNAVVLASDSPEDFAKGMEEVLAIDEKQYLVMRKEARVSAETKLDISVYSEKIKE
                     LLTKKSPTEE"
     gene            5899..7068
                     /locus_tag="PLU08_03675"
     CDS             5899..7068
                     /locus_tag="PLU08_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013858318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOQ70823.1"
                     /translation="MKKIVVIGLGYVGLANAVLLSLNDEVVAVEINEQRIDLVNKRIA
                     PIQDAEIEHYFANHKLNLVATSDLAWALKDAEFVVIATPTDYDTNKNFFDTSSVDSSI
                     EKIHKINKNVIVIIKSTIPVGYSENIQKKYSDMEIIFSPEFLREGKALYDNLYPSRIV
                     IGSHSPQAHAFAELLKKNTLKEDVQVLFTGPTEAEAIKLFSNTYLALRVAFFNELDTY
                     AEIKGIDTKQVILGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYKGVPQNIIGA
                     IVDANRTRKDHVADMIVAKNPKVVGVYRLVMKNNSDNFRSSSIQGVMKRISEKGIKVV
                     VYEPALAAKDFFGSEVIRDFDEFVKMSDLIVANRHYKELAEVSDKVYTRDIFNRD"
     gene            7086..8120
                     /locus_tag="PLU08_03680"
     CDS             7086..8120
                     /locus_tag="PLU08_03680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ70824.1"
                     /translation="MAKNNIGVIGFYKKGEQFNSGQIVKTVSVVDALKELPDTKVYVM
                     NTYKWKYRGIFLFVKIFFSFFKCKNIILITAQKGIRVFTPLLSFFNKIFRWRIHYIVI
                     GGWIGKKVAKQKALKRIGKFYQVYVETTKIQEELASLGITNTTILNNFKDLPFASPED
                     LKKEHAVPYNICYFSRVEPEKGVEDIINVLANINKDKLLYKLTIYGVIRPNYKEDFLA
                     FVEKHKDYVEYAGVIKYDQSVNTIKDYFLQIFPTQYPTEGIPGSIIDSYYAGVPVVAA
                     RWNSFHEIINEGETGLGYSLGNLEELEKTLLKVIAEPNIITNMKGNCQQKAHEYSKEH
                     FLATFPQHFL"
     gene            8133..9047
                     /locus_tag="PLU08_03685"
     CDS             8133..9047
                     /locus_tag="PLU08_03685"
                     /inference="COORDINATES: protein motif:HMM:NF018378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HOQ70825.1"
                     /translation="MKLRNFTFRNIRKWFRMVTGRSILHVNQGPGKHYSLTEVKGYYN
                     DLTEKITKDKRAAENYIPKQKNAKNDEFAFSIEIFQYGLAAYDLFLETNEEIYKEKFL
                     LMVEWAMNNQKDNGGWNTFVERHPEHPYSAMAQGEGISLLVRAYVLTQDDLYLAAAEK
                     AFNFMKLPVEKGGTTLYTDDLVLLQEYTHLPTVLNGWIFALFGVFDYYLMTKDEDVNK
                     FFDYNVRSIASALPQFDNGYWSKYDIDQKIASPFYHQVHIAQLRVLSELTNNKTFTEF
                     ADKWEGYASKRKNRRKAFWRKARQKLSE"
     gene            9096..9857
                     /locus_tag="PLU08_03690"
     CDS             9096..9857
                     /locus_tag="PLU08_03690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ70826.1"
                     /translation="MNTTTPPLVSIITPLYNCEDIIRETIGSVLAQTYPHWELIIVDD
                     VSTDNSREVVKEYVAKDKRIKLIELSENGGAAIARNKGIEVAEGRFIAFLDSDDLWKE
                     TKLEKQVNFMLENDYAFTCTDYEQLVDDTKKIKLIIKARVKADYRIVIRYNPIGNSTV
                     MYDTLKMGKVHIPEVRKRNDYALWLKILRMEKYVYGINEVLATYRIRKKSLSRNKVKL
                     IKHQWYVYRKLEKINFFKSLYILWFVIITKIFRIK"
     gene            complement(9928..11103)
                     /locus_tag="PLU08_03695"
     CDS             complement(9928..11103)
                     /locus_tag="PLU08_03695"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOQ70827.1"
                     /translation="MYNKEEIVNIGKQAYDKVDSVANLAKKYGVAVASIYNWKRLYAQ
                     KQGLVKLHEETNYETMNEAQLKLELMKKDIEIARLKKRLRGERGWGKEGIRYYTRLEY
                     QIVAELSYKYEVKKLCRAMNVSRSGFYIWQERVNRPSEKVLQRARDIQLFRKYHSRHQ
                     SHGYRWLNHKIRLDLGIFMSDQYAHKCCKIAGIKSKARHNRYKRPGNEGKTYPNLILS
                     NMNINRPLEVIVSDMTAMYIKGKYHELTLYMDLYNNELIGHSLSDKRGDPNTYHDGLK
                     QVLNKKEEQRNLDMILHSDQGSVYSSKSYNELLPNYNIKRTMSRAGTPTDNGAMEAIN
                     GWLKEELFLDFKINEADNIEKAINDYIHYFNYERPACALNYLTPIQYKVKYLLNSNQ"
     gene            complement(11278..12111)
                     /locus_tag="PLU08_03700"
     CDS             complement(11278..12111)
                     /locus_tag="PLU08_03700"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HOQ70828.1"
                     /translation="MIVFRKKDKIKQLDMLNFKLERELQNFIEENLKEIFQFEFLATE
                     YPINNFRFDTLAYDRENNTFTIIEYKRGKNESLVDQGLAYLSTLLDRRSDIVLLFNKV
                     FEANKQIEDFEWDLVRVYFVSTQFTEFQRAATSFKDMPFLLFEVKRFEGDLYVISEVL
                     TKSKNTTPKVGNGPVVPADIKVYTEDDHKQNIPARVVELYDEVRDRLLELDDVEIKPL
                     KIYIAFKTLNKNFCDVKIQKDSLLVYLNLPHGRLDDAYNKTRDVSKIGHHGNGDYTFK
                     L"
BASE COUNT         3700 a         2183 c         2265 g         4078 t
ORIGIN      
        1 taggcgtcct gtaattcagc gatgaatgat atcttttatt gttataccac caaacataac
       61 tgaatacttc ttattctaat tcctttatat ttttaaatac ttttcctcct aatgcttcga
      121 ttttggcttt acgtctttgc ctattggtat acggccgcta tcgtcctcgt actatttctt
      181 gcttttgccc ttattgggct tacgattttt gaaagatatc ctttctacca tgacttacta
      241 gcagaacgca gtaaaggcga agaaaaaaga agccttacca ttgcctttct cgtctttatt
      301 atggcaataa atatattttg gaggatatac ggtgaagcct ataaatatat tatccttgcc
      361 ggcgttttag ggtggggcat cggtgatgcg cttgcggctt tagatggaaa acaaaaaatt
      421 agtattgtta ttgaaacata aatgctttct accaaaaaga caattcaggg catttttgca
      481 atgtttttag gttcaaccat cgtcattttc cctgtccttt tccttgttgg cagcaaagat
      541 tggatatata gtttgattac tgctcttgtc gccggaatta tcggcacttt tgtcgagtta
      601 tacacaaaag acgggtggga tacacttact ttgccatcag cgttaatggt tattctttat
      661 gtcgcaagta ttatagttca atagtcaacc ataacattta aacacagagg aaatatgtac
      721 aaaaacaggg caaaattaac ctctttttca tttaaaggtt attatttctt aatataataa
      781 tagacaaaga tgcggaacat gatataatat gttatctatg aataacataa ttagcgagca
      841 tatcacagta gaaaaaccaa cacttgtaaa gttttggcag aagtataaag caaagatttt
      901 ttcagcatca gtaacgacgc ttattgtcgt tttacttttt ttatttagcg gtaatgctta
      961 tgtcgaattt gctatatcga agcggctaga agccttatta cttctttcac tattaggcat
     1021 ttccggcatt ttattcttgt taaacattag ttttgtcaag gttaaaaact atgttttaag
     1081 ccttaagagc aatctaaaaa acaaaaagat aattctgacg cttgttttta gtgcggtctt
     1141 tattgggtta gcagcggcta ctttaattat taacattgtg tcgaatcgtg gcaattttac
     1201 cggtattttg cgaatgattt cggtatacgc aacgattgtg atgctggtcg ttacttataa
     1261 gaaagagcgc ataatcgata catttctatt atcggtgcgc attttaatta ttgcttcctt
     1321 agccgtattt attctttcgc tttttttacc atcacactat gttaccgaaa tttatgttac
     1381 gaaggctaat acgctcgtct ataactattt ccacctaagt tttattttgc gcagttgggg
     1441 ttacattagc gcaggtattc cgcggtttag ttccatattc tgggaaccta gtatcgccgc
     1501 tggtatattg tcaattgcct tatttgtgcg tatcttttac aagaagcata gtatgtttga
     1561 tactttttta tatgtgatag ccgttgtatt tacattatcg accggcggta tttttctttt
     1621 tggccttctc tttatttatt acattatgac ggtattagat aagcgggaga agattgtttt
     1681 tggctcaatt attttagtag gatcaatttt tgcctttatc tttagggaat caatcttttc
     1741 ggctttagcg acattatctc cgcagttatt tgggaagtta tcaacgcaga gtacaccgtt
     1801 tttaacgcgc ttaaattcat taccaacctt tattaaaatt tttggtgaaa agcctatctt
     1861 tggtcacgga ccttttggcg cgattactcg ttactatgaa gttattgccg gttcgtccct
     1921 tgatgctgca acttcaacct ttggcttctt aattgcggcc tttggttcta tgggtgtctt
     1981 tctcgtcgcg cttattatta ccgcgccatt atttttacag gagaaatgga ctaaccgtat
     2041 tttcgcttcg ctctttttca tcgttttact taacttacaa aacgtcccct tcctcgctct
     2101 ctttatgctt ttcccggtca tctttattct tgaaggtgtc tttaataaaa agacttcgtt
     2161 aaccgagaat aatcccgtta atgaaagagt gtacgcttat ttctttatgt cgaagacgga
     2221 aggttcagcg gtcgttaaaa atattaatgg tgctttaatt atcaaaggct tatcaatgat
     2281 cgtaggtatt attgctatcc cagtatatcg aacgtttttt attagtgaag aatattatgg
     2341 cctttggatg gcgattatct ctattcttac gtggatctta ttctttgact ttggttttgg
     2401 gattggccta cgggctaagt taggaccact cttagatgcg ggcgaagaaa aagatgcgca
     2461 agaaaaaata tcatcaacct atgttggctc aatctttatt gccttaggat tattagtcgt
     2521 ctttgcggtt ttaatatggt cacttgattt aaatggcatc ttaaatacac cgacaagtct
     2581 tgtttctgaa atgcaattaa aaatatcgat gtctatttta gcggccggct tttgtattga
     2641 gtttgtcctt aaaaatgttt tgtttgtgct ctactcaatg cgtaaaagtg cgattgcatc
     2701 gtttgtcacg ttcctaagca tgctaagtct tttacttttc tttatcattt tcaaagactt
     2761 taatatcact aatcgcttac tcgtttcgtc aattgtctat gtcgtttgta ttaatgtccc
     2821 ccttattgtg gcttcatttg tcttatacgc ccggaaaaaa gccttattac caacgtttaa
     2881 aggatttagc ctagcgtcaa tgaaaagtgt catgtcgatt ggtctcgtct ttttccttat
     2941 tcaaattggt ttcttgttta ttacccaaac tgatggtctt ttaattacaa gcatgttctc
     3001 tactaacgcg aacgcgcaat atacttatta ttcgcgaata ttcgttttct ttgttggcct
     3061 tatgggcgcg gtcgttcaac aaccgatttg gtccgcgatt tcggcggctc gggaacaagg
     3121 cgacacgaaa atgataaata aatatattaa agtgacattt ggcattgcct tattcctttt
     3181 tgttatttgt ctattcattg gcgtgggcct gccctttatc tttgagttat ggcttggtga
     3241 tcatgccccg acggttaata gtttctttgt tattgtattt ttgctttact cctttatttt
     3301 cttattctca aatgcgctta ttattgttgc taacgggcta acaatattaa agagccaagc
     3361 agtcatcacg ttaattgtcg cggctcttaa aatacctgtt ttaattggtt taatgtattt
     3421 attgccccag cttgaatgga gcgtggtcgt ccttgttacg acgctttgct atgttcctta
     3481 tcttattttc ctccccatat atattcataa aatgattaag aaggtgcgtc atgaagaaaa
     3541 aatcgcttaa atacattttg tttatgctta gtgccctaat cgcgcctgcg cttttatcgg
     3601 tcggtgttgc ccttgctgac gttaatttta attattttgt gatgagcaat gcggcaaagt
     3661 atattaaaga aggcgaagaa aagatgcttg ttcgccttga taataaagac gaaattaatg
     3721 aagattggcg cagttttgcc acaacataca accgccgcca tcaaacgtat tcatcgatct
     3781 ttagacatta tagcgatgtt gttgtttcaa ctgctggtag tgagcgaaat gttcatcttg
     3841 ctgatattaa tttgcggagc tcaaatgtgg ttgatactat tgaaggcatc aaaattattg
     3901 atatgggtca tagtttccaa gacttactta ccgctgatca ttcactcgtt atttcaacat
     3961 tgtacgccac gaccttaatg aatgaaaaag gcgtaagtga aatgaaagat ctccttaatg
     4021 ttgaagttaa cgtaacccgt gataatttaa cgattgttca taaagtgcgg gcgatatacg
     4081 atgtgaatgt taaagatgaa tacgctaata ataagtatta tgaaacattt ggtgatttat
     4141 tctttgttat tcgggagaat actccacttt ttgataatcc tgagtattac ttaacccttg
     4201 gtcgcgatca agaccataac attaccattt atgatgactt acaatattta aaaactaagt
     4261 gggcggctaa tgtcgctaaa acatcaattg atagcgaggc gcaaaccctc attaataagt
     4321 atgatgagct tattaaagat gcttcattta atttacttag ggcttccttt attattgtgg
     4381 gcgcactagt gaccatcttt aatattgtcg taattacttt aaaagtaaaa caagggaaaa
     4441 aagactgggc gttaaagaaa tttgatggtt ggaccattgc cctattaatc gttatggggg
     4501 ccccgctttt actaatgctc gtgacaagta acataccctt atttggatta atacttaatc
     4561 gtcttacccc gttaggtatc gcgctttacg tactttcagc cttaagtgtt tacttagcct
     4621 acaaagcact atgtacgtag caagaagcgg aagtgagcaa ggaaaaggag aactaagata
     4681 tgaaaaaaac atgtgtaatt gtatcgacaa tgtattgtcc aaccggatcg gcaagcgggt
     4741 tacgtatttc ttcagtggtg gatgttttaa tcactctagg atatgattgc catgttattg
     4801 gtttatctga ctatgacgtc tttaagaccc acgaagtaaa acccggttac accgtgcatt
     4861 caatttacaa tattaaacgg aaaaataatg cttttgaaaa attaaaggtg tttttgtatc
     4921 ccaaaaaacg ctttaaaacc attattgaaa agattaaaca ggaacggaag attgaccttt
     4981 taattatata tcaacaatta ccgctgcaat catcacgtta tattttaaat gtggcgaaga
     5041 aagaaaaaat aaaactatta tacgattttg tcgagtggca agttttaggc caacaaaatt
     5101 tagtttccta ttttactttc tatcgtccta atcaaaaata tattaaaaag tattcaaagt
     5161 acggcttaaa tattgccatc tccaccttct taaatgagta ctttctatca caaaacattg
     5221 attcactgta tgttccgttt ttctttgatg ttaaaagtaa agtctatgtc gaagataata
     5281 aaccatttaa acctaatcaa gacaagatat atcttggcta ctttggtaaa ccttttgggg
     5341 ggcgcgacac aattattaat gcgattaaag gcatcttgtt attgccaaag gaaaaacaag
     5401 caaaatttga attcattatg gcgggtccaa atgataacga tttaattaaa ctaggattaa
     5461 cgccagctga acttgaagcc gcgcaaccat ttcttaaagt cttaggccgc attccccatg
     5521 agcaattagt tgatatatat aaatatatag actatactat tttgctcaag cctgccgaaa
     5581 aaagattttc caaggctggg tttccgacca aggtttcgga aagcttatcg tatgcggtgc
     5641 cgtctatttg taacttaacc ggtgacttag aattattctt agagcacgaa aaaaacgcgg
     5701 ttgtccttgc ttctgattcc cccgaagact ttgctaaagg gatggaagaa gtattagcga
     5761 tagatgaaaa gcagtattta gtgatgcgta aagaggctcg cgtcagtgcg gaaacaaaac
     5821 tagacatcag tgtctatagt gaaaaaataa aagaattact cacaaagaaa tccccaacgg
     5881 aagagtaagg aggagaaaat gaaaaaaata gtagtgattg gtcttggcta tgttggcctt
     5941 gcaaatgcgg tcttactttc attaaacgat gaagttgttg ccgtggaaat taatgaacaa
     6001 agaattgact tagtaaataa gcgtattgct ccgattcaag atgcggaaat cgaacattat
     6061 tttgcaaacc ataagttaaa tttagtggcg acaagtgatc ttgcctgggc gctaaaagat
     6121 gccgagtttg tcgttattgc caccccaact gactatgaca cgaacaaaaa cttctttgat
     6181 acatcttcag tggatagttc gattgaaaaa atacataaga ttaataagaa cgtgatcgtt
     6241 attattaaat ctacaattcc tgttggttac tctgaaaaca ttcagaagaa atatagtgat
     6301 atggaaatca ttttctcgcc tgaattttta cgggaaggta aagccttata cgataactta
     6361 tatccttcac gaattgtaat tggcagtcac tcaccacagg cgcatgcttt tgctgaactt
     6421 ttaaaaaaga acacgcttaa agaagatgtg caagtattat ttaccggacc aaccgaagcc
     6481 gaagcgatta aactattctc taatacttac ttagcgctcc gtgtcgcatt ctttaatgaa
     6541 ctagatacgt atgcagaaat aaaaggtatc gatacgaagc aagtaatttt aggtgtcggc
     6601 cttgatcccc gcattggcag tcattacaat aacccttcat ttggctatgg cggctactgc
     6661 ttaccaaaag acacgaaaca attattagcg aattataaag gcgttcccca aaacattatc
     6721 ggcgcgattg tcgacgcaaa ccggacacgc aaagatcatg tcgcggatat gattgtcgct
     6781 aaaaatccta aagtagttgg tgtctatcgc ttagtaatga aaaataattc tgataacttc
     6841 cgttcatcct ccattcaagg agttatgaaa cggattagtg aaaaaggtat taaggttgtt
     6901 gtctatgaac cagcgcttgc ggccaaagat ttctttggct ccgaagttat tcgtgacttt
     6961 gatgaatttg tgaagatgag tgacttaatt gtcgctaacc gtcattataa agaacttgcc
     7021 gaagtaagcg ataaagtata tacccgcgac atctttaatc gcgattaata aggaaagata
     7081 aagacatggc aaaaaacaat attggtgtta ttggcttcta taaaaaaggt gaacaattta
     7141 atagtggcca aattgtcaaa accgtcagtg ttgttgatgc cttaaaagaa ttaccggaca
     7201 cgaaagttta tgtgatgaat acatataaat ggaagtatcg cggtattttc cttttcgtta
     7261 aaatattttt ctcctttttt aaatgtaaaa atattatttt aattactgcc caaaaaggca
     7321 tccgtgtttt caccccatta ttatcatttt tcaataaaat atttagatgg cgtatccatt
     7381 acatcgtcat cgggggatgg attggcaaaa aagtcgccaa acaaaaagca ctaaagcgga
     7441 ttggtaaatt ttaccaagta tatgttgaaa cgacgaagat tcaagaagaa cttgcaagtc
     7501 ttggtattac gaacacaaca attcttaata actttaaaga cttaccgttt gcgagtccgg
     7561 aagacttaaa gaaagaacac gctgtgcctt ataatatttg ttatttctcg cgggtcgaac
     7621 cggaaaaagg tgttgaagat attattaatg ttcttgcaaa tattaataaa gacaaacttt
     7681 tatataaatt aacaatatat ggcgttattc gccctaatta caaggaagac tttttagcgt
     7741 ttgtcgaaaa gcataaggac tatgttgaat acgcaggtgt tattaaatat gatcaaagcg
     7801 ttaacactat taaagattac ttcctacaaa tcttccccac acaatatcca accgaaggca
     7861 ttccgggctc aattattgat agctattacg ctggggttcc ggtggtagcc gcgcggtgga
     7921 atagttttca tgaaattatt aatgaagggg aaaccggact tggctactcc ttaggtaatc
     7981 tcgaggaatt agaaaagaca cttttaaaag taatagcaga accaaatatt attactaaca
     8041 tgaaggggaa ttgtcagcaa aaagcccatg aatattcaaa agaacacttt ttagcaacgt
     8101 ttcctcaaca ttttttatga ggcaataagg atatgaaact acgaaatttt acttttagaa
     8161 atattcgcaa atggtttagg atggtaaccg gccgtagtat tttacacgtc aatcaaggac
     8221 ccggtaaaca ttattcacta accgaggtaa aaggttacta taatgactta accgagaaaa
     8281 tcacgaaaga taaaagagcc gcagaaaatt acattcctaa acaaaaaaac gctaaaaatg
     8341 atgaattcgc ctttagcatt gaaatctttc aatatggcct tgctgcctac gatttattct
     8401 tagaaacaaa cgaagaaata tataaagaga agtttctttt aatggtggag tgggcgatga
     8461 ataatcaaaa agataatggg ggatggaata cctttgtcga gcgtcacccc gaacaccctt
     8521 attcagccat ggctcaaggc gaaggcatat cgcttttagt aagagcctac gttctcaccc
     8581 aagatgactt gtatctagcg gcagccgaaa aagcctttaa ctttatgaaa ttaccagttg
     8641 aaaaaggggg tacaacgtta tacaccgatg acttagtgtt acttcaagaa tatacacact
     8701 taccaactgt gcttaatggt tggatattcg ccttatttgg cgtatttgat tactatttaa
     8761 tgacaaagga tgaagatgta aataaattct ttgattataa tgtgcgttca attgcaagcg
     8821 cccttcccca atttgataat ggttattggt caaaatatga tattgatcaa aagattgcaa
     8881 gtccttttta tcatcaagtt catattgcgc aattacgagt attaagcgaa ctaactaata
     8941 ataaaacctt tactgagttt gccgataaat gggaaggtta tgcttcaaaa cggaaaaatc
     9001 gtcgtaaagc tttttggcgc aaagcccggc agaagttaag tgagtaatac cattatgaaa
     9061 atgaagatat ataaaacttt aagaggtaaa agactatgaa taccaccacg ccccctttag
     9121 tgtcaattat tactccgctt tacaattgtg aagatattat aagagaaaca attggttccg
     9181 ttttagcgca aacttatccg cactgggaac ttattattgt tgatgatgtt tcgaccgata
     9241 actctagaga agtcgttaaa gaatacgtag cgaaagataa acgtattaaa cttattgaac
     9301 taagtgaaaa tggtggagct gcgattgccc gtaataaagg tattgaagtt gccgaagggc
     9361 gctttattgc ttttttagat agcgatgact tgtggaaaga aacgaagtta gaaaaacaag
     9421 ttaacttcat gttagaaaat gattatgctt ttacatgcac tgattacgaa caactagtcg
     9481 atgacacaaa gaaaataaaa ctgattatta aagcaagagt gaaagctgat taccgcattg
     9541 ttattagata taacccaatc ggaaactcaa cggtcatgta tgatacttta aaaatgggaa
     9601 aagtacatat tcctgaagtg cgtaagcgta atgattatgc tttatggcta aagatattaa
     9661 gaatggaaaa atatgtatat ggtattaacg aagtcttagc aacctaccgc attcgaaaaa
     9721 aatcattatc ccgcaataaa gtgaaactaa taaaacacca atggtatgta tatcgtaaac
     9781 ttgaaaaaat aaatttcttt aaatctttat atatcttgtg gtttgttatt attactaaaa
     9841 tatttagaat taaatagcca attgcgctta aataatagaa tctttgcacc agtcaagcaa
     9901 aagtagacac gatttttaaa taattagtca ctgatttgaa tttagtaggt atttaacttt
     9961 atattggatg ggtgttaaat aatttaaggc acacgctggt ctctcataat tgaagtaatg
    10021 gatgtaatca ttaattgctt tttcaatatt atctgcttca tttattttga aatccaggaa
    10081 taattcttct ttcaaccatc cattgattgc ttccatcgcc ccgttgtcag ttggagttcc
    10141 cgctcgagac attgttcgtt ttatattata attagggaga agctcgttgt agctttttga
    10201 agagtatacg gacccttgat cagagtgtag aatcatgtcg aggtttcgtt gctcttcttt
    10261 tttattgagg acttgtttta ggccatcgtg ataggtattt gggtctccgc gtttatcact
    10321 aagactatgg cctatcagtt cattattgta taaatccatg taaagggtta gttcatgata
    10381 tttccccttt atatacatcg ctgtcatgtc acttacaatg acttccagag gcctatttat
    10441 attcatatta ctaagaatga ggtttgggta tgttttccct tcatttcctg gtcgtttata
    10501 tcgattatgc ctggcttttg acttaattcc cgctatttta cagcatttat gcgcatattg
    10561 atcactcatg aagattccca aatctaatcg gattttgtga ttgagccacc gatatccatg
    10621 gctttgatgc ctactatggt acttacggaa taattgaata tcccgcgctc gctgaagtac
    10681 tttttcgctc ggacgattta ctcgttcttg ccatatatag aatccactgc gagaaacatt
    10741 cattgcccga cataacttct taacttcata tttgtaggat agttcggcga caatttgata
    10801 ttctagtcgg gtatagtatc gtattccttc tttcccccac cccctttcac cacgtaacct
    10861 tttttttaat cgggcaatct caatatcttt tttcattagt tcaagtttta attgggcctc
    10921 attcatcgtt tcatagtttg tttcttcatg cagctttact aacccttgtt tttgagcata
    10981 taaccgtttc cagttataaa ttgatgccac tgccacgcca tactttttgg ccaggttcgc
    11041 gacactatca actttgtcat aggcttgttt gccaatgttc actatttctt ctttgttgta
    11101 catgtttcct ccggtacatc catcttatca aaactgggtg cacccagttt tttataccgt
    11161 gtctacattt agcttaccgt tcagtttggt ggtataacaa taaaagatat cattcatcgc
    11221 tgaattacag gacgcctatt gaatataaaa gtgtctgtct caataagtgt tgacatctca
    11281 cagtttaaaa gtataatctc cgttaccatg gtgaccaatt ttacttacat cgcgagtttt
    11341 attatacgca tcatctaatc taccatgcgg aaggttaaga taaacaagca aactatcttt
    11401 ttgaattttc acatcacaaa agtttttatt taaggttttg aacgctatgt agattttaag
    11461 tggtttaatt tcaacatcat caagttcaag taaacgatct cgcacctcat catataactc
    11521 gacgactcgg gccggtatgt tttgtttatg atcgtcctcg gtatagactt taatatccgc
    11581 ggggacaaca ggaccattcc cgacttttgg agttgtgttc ttactttttg taagtacttc
    11641 actaatcacg tagaggtcgc cttcaaagcg tttgacttca aataagagga acggcatatc
    11701 tttaaagctc gtcgcggcac gttggaactc cgtaaactga gtggaaacaa aataaacccg
    11761 gacgaggtcc cattcaaagt cctcaatttg tttgttcgct tcaaacactt tattaaaaag
    11821 gaggacaata tccgagcggc ggtctaagag cgtgctgagg taggctaaac cttgatcgac
    11881 gagactttca ttttttccgc gtttatattc aatgatcgta aaggtgttat tttcccggtc
    11941 ataggcgagc gtgtcaaagc ggaaattatt aatagggtat tcggttgcta aaaactcaaa
    12001 ctgaaagatc tcttttaggt tctcttcaat aaagttttga agctctcgtt cgagcttaaa
    12061 gttcagcata tctaattgtt ttattttgtc tttctttcta aaaacgatca tcgtgcgccc
    12121 tcatttctct aaatgtatat attcttactt cgcaagtgaa ctaacacttt aataatagca
    12181 aatatggggg agaaaaaccc gatttttggt atttccacct atcaac
//
LOCUS       DAOSTA010000030        11986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_5298_length_11986_cov_9.458386, whole genome shotgun sequence.
ACCESSION   DAOSTA010000030 DAOSTA010000000
VERSION     DAOSTA010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 11986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11986
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5298_length_11986_cov_9.458386"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1805)
                     /locus_tag="PLU08_03705"
     CDS             complement(171..1805)
                     /locus_tag="PLU08_03705"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOQ70829.1"
                     /translation="MKKLFKGLFTTALAVGLLAGCKGGASSSEDTRAKHSVVLKNYDG
                     TYIDTLQIREGDKATKPADPAEFKPGDDFTFRGWHKEARLQLGEDYEAYDWDTPVTSE
                     VKLLALWEGSDVYVTFYVNELAVIHEEIKVKAGHILLEMPEDPVRDGYEFLGWYSDEA
                     LKKPFKPTDFIMEDTDVYPKYEKLYVPDTRTFHVVGSLANPDFSYINWNAVGEEGDDW
                     DVRSYLTKAEDSNLYSIELVIGFRGEFKIKIPNAEWDSELQFSYGKIREEDRNDENFL
                     AGGFDNVQIVNAGAYLIEIETTYEWLRVTRTGDLPEGSPATPNPEEGAIDNWGVVGEL
                     NNWGNDGDLDIELTYNDDNLEAPFYYIPFVKLPVGDFKLRANNDWGLNFGSHPDNVLP
                     AGFTQPTELVEEVETIKTGENITVTEGHEGWYRIYFDTTKIVITEINFALRGDSLADG
                     WYKDSAPLAVAGEVAGEAGAVTLTLEGTYTFTDGKFKVKLGALAVGETELYSGWSLSF
                     GDSEGQDIPVTAGDHKVTLTLEIAADYTFTGAVTLA"
     gene            complement(1995..4835)
                     /gene="pulA"
                     /locus_tag="PLU08_03710"
     CDS             complement(1995..4835)
                     /gene="pulA"
                     /locus_tag="PLU08_03710"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HOQ70830.1"
                     /translation="MKKLFTILLASLMVFGSTDKSAPKRAVGPKPTLEVEKVMTPEQA
                     YAEPHIVFHYFRNDANYARYAMWIWGQDEDGSEYNFTETDAYGKYLHVPVANFTDQTQ
                     INFLLKTKGSWEWQTPDVNIQYSDFTFDTVTNAYHFYLVNGLITVFASAEEAKANQVT
                     ESIFASKNTVRVSTNNEPTTFVIKADGTNVTSGAAEHTYNNNTWSYVFTVNLGAAFLP
                     DFSKAYTVEVAFTAGSAVISEIGLSGLFDDPYFIENMTYNGQDLGVTYSPTKSTFKAW
                     APTASELKLRIYESGTPVALNADLGNDTYTEHDFVRTEKGVWSAELAGDYHGKYYTFV
                     AKHPTGYVELIDPYAKSAGINGVRGMIVDFSKTNPTGWDEVDYPVKSHTEIIPYELHV
                     ADLTADESWNGSEENRKRFLGLIEEGTTYTAGGVTVKTGFDHIKELGINALQIIPFYD
                     QANDEREPSFNWGYNPQNYDVLEGSYSSNPYDGLVRIREFKQVVKAYAAEDIRIIMDV
                     VYNHVANLGGHSFNKLVPGYYFRYNADGSPTSASGCGNDTASERIMMENFMRDSTAFW
                     TKEYKIGGFRFDLMGLHTVKSMNTVRNRLVEIRPDIFIIGEAWDMGGGSVSANGANQN
                     NIHKVPGVGAFNDKLRDSVKGDTNGKARGFVQQAASEIYQGTKNSIKDGMLGKISGTQ
                     SSPVQTVNYVACHDNLALYDKLVNAGGHLFGLDQLAQQNIQAEAISLLSQGVSFIHAG
                     SEILRSKPLGDGKFDHNSYQSPYSVNSIKWNEKVTNLNVFERYKELIAIKRDTPGFHY
                     TTSAEISANVSVKFGSEIGYQDTLIKLVVVDGETTYTAYFLGAGTRVRINDLEGQYVL
                     FDSTNTMEKWSVLGKSVSLSSNATIVTVSGEGVTPPATSTPDLPSSETLPSEEGEEAN
                     LTWLYITIPSLTVVGVGVGLILLFFRKKKLSV"
     gene            complement(4852..6315)
                     /gene="malQ"
                     /locus_tag="PLU08_03715"
     CDS             complement(4852..6315)
                     /gene="malQ"
                     /locus_tag="PLU08_03715"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOQ70831.1"
                     /translation="MRQAGILLAVSSLPGKHGIGDFGPSGRTFIRKASKTGFKLWQIL
                     PVNPLGYGNSPYQPFGSKPFDEIYISLELLTKEGLLPEVPNYFARSKKVRYDKVRKFK
                     LRHLKRAFNNFKKQGRRGFKKWVKDNKWVKDYAIFITFKRDNNLVQWHYWKEAQREWS
                     KTRRGVNLKPFAKAIEFEMWLQYIAYKQWMDLRAYAHRYGMQIVGDIPIYVGIDSVDV
                     WTNRKAFLLDENDEQTFVGGVPGDYFNADGQLWGNPLYDWDYLAETGFEFWVDRLRYN
                     ARLFDIIRIDHFRGFDSYWKIPAGSSRANDGHWAYPPGYALFDKIYEELPDIKIIAED
                     LGDLRPEVLELRDHYNLPGMEITSFTFNPFEKRDENAWLENRCIYPGTHDNSPIRGYF
                     KWKDKKFMKAANKLLDDLGFNEFDNFYDNFMAMNFGSDARYAIFQMQDLLRLGDEAKM
                     NSPSTIGSPNWEWKMQDFKTFDEESSLYAYLIEKYNR"
     gene            complement(6317..7924)
                     /locus_tag="PLU08_03720"
     CDS             complement(6317..7924)
                     /locus_tag="PLU08_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOQ70832.1"
                     /translation="MKAKQSLRALMLIFPLLFMASCKDLPSESVSSEEILQAPTDYEI
                     DESITNEKGGVYYQIFVRSFADTNADGHGDLKGITDKIPYLQELGVKGIWLTPINPSP
                     SYHGYDVTDYKAIHPDFGTLDDFDTLISVAAEHNIDVIIDLVINHTARTHPWFLEGRR
                     RFNTPEYNPNDPNDKGNWYDFYIENGEVKVKIGYFGDHMPELLLNNTAVREEITSISK
                     FWLDRGVKGFRLDAAGHFFETHPENIDFLGWFSDMVKSYKPDSYIVAEAWENFNVQKY
                     YYAGVESLFNFDGANATGFIIDSIMARSGPQLSHYISNAFSDAYAINENALMAMFLTN
                     HDMDRSSQMFTGDQLERQKLGASVYILTPGVPFMYYGEEIGLKGSRQGAMTDANRRLP
                     MIWKRVGDKHRTNPPIGTTYNMANQVKYGAEDLLSEPFSLLNHYRKVINVRNFHPWIK
                     NARAYNLRLNNNGVTGLTLKEHNGQRELHVIHNLLAEEVSLNLTRFISEYEVEIIHDI
                     YTTGTRAKYVDGSLTLAPYSSVVLRRK"
     gene            complement(7927..8751)
                     /locus_tag="PLU08_03725"
     CDS             complement(7927..8751)
                     /locus_tag="PLU08_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010772099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ70833.1"
                     /translation="MCVAWIFPIIWLIANALRIESTGPVSYLFPREIGFDNFRRLFTT
                     AEMPYLKWFTNTLIVATFSSIISTTIVLLTSYTFSRLRFKARKGLMKFLMILGMFPGF
                     MSMIAIFHILKAIGLLPTETRPEVPVSNYLISLILVGTSGAAMGYLVSKGYFDTISKS
                     IDEAAEIDGANKAQIFWHIILPLSKPILIYTALGAFMGPWGDYIFVATITRGKREAWN
                     IARGLYDMINGGLEKLGKYFTLFCAGAVLISIPITILFVFMQRYYVSGVTGGAVKG"
     gene            complement(8915..10570)
                     /locus_tag="PLU08_03730"
     CDS             complement(8915..10570)
                     /locus_tag="PLU08_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ70834.1"
                     /translation="METSDKKVKSAHSRTAGTWFKDFLSTIGGFFVRLGKGIVNFFID
                     TGIAIKNTFVNLWKMIKNFFVSVYTHFVKGGWRTKLSHFIMGSGQMSMGRPIKGIIYL
                     LIQVAYFIFMFVPGGGLAWLIKFRTLGEVAVEYMAECTWPPGAPIEDCDPDFITYEKP
                     IFKDNSLLIMLFSVATILITVFFIVMYFVQVRGTFKAEKAYNEAKAKYELAKSEGSEE
                     AFKNPLPKFTDELKSLLNERFHFTTLSLPAITLTMFTILPLVFMILLAFTNYNRDTAP
                     PNKLFTWVGLDTFKNLFSTKGASATNATFGSAMVRILKWTFTWAFFATFTNYIFGLIL
                     AMIINRKSIKLKKLWRTIFVISIAVPQFVTLLLMNQLLKEYGPLNETLLQWGWIRQRI
                     KFLDDPVLAKITVIVVNIWVGVPYTMLLTSGILMNIPTELYESAKIDGAGPVRQFFQI
                     TMPYILFVTGPYLITQFIGNINNFNVIFFLTGGGPSTGIPGVPYGHTDILITWLYALT
                     VGGAHEEYAIGSALGIFIFLISATITLVLYSRTSAAQSEGDFA"
     gene            complement(10672..>11986)
                     /locus_tag="PLU08_03735"
     CDS             complement(10672..>11986)
                     /locus_tag="PLU08_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ70835.1"
                     /translation="EFMFKQKRIALLVAGLLLLAGCNGGSSADVSGDVSQGTTSQTTT
                     TSGGGSKERVRLSVWGSSAEEPLYHKHATEFQEKHPEINFSISYADVGEADAATNVLQ
                     DVSTAADVFFFADDQLPTMQAREVIAKLPKGYANKVKERDVASAAENASSAVDDELYA
                     FPVTNDNGYMLIYNKALLTETDVETLEGIMAKTTAEHQFVVDMGNGYYATSFIQYLCD
                     ITYNPITRLHTTNFNSAEAVDALQGVSNLLKPRVDNGFKSVDFNGAALDDLSDANGNK
                     VIAAVTGVWNTELLKEKLGENLGATKLPTFKSANGTTVQWGSFAGSKLVGVKSSSNNL
                     GWALAFADYITDEAAQRYRFETNGWGPSNKNLLADEELLADHVTLKALGAQAPYAISQ
                     AKSVGSKFWDNAAIVGNFLWKGPASEESPQTLQATLDAFVDALTK"
BASE COUNT         3460 a         2477 c         2324 g         3725 t
ORIGIN      
        1 acgcccgact tacagtttcg aagactgttg ctctatccaa ctgaactacc gggacacata
       61 tttagattaa tctaaataat actaaatttc aaggtgacta ttaaaaaagc ccgctttttg
      121 ttaagcgggc taaaccattt aggattggta atttatttta gttatgccta ctaggcaagt
      181 gtgactgcgc cggtaaaggt gtagtcagca gcaatctcta aagttaaggt aactttatga
      241 tcaccagcgg ttactggtat gtcttgacct tcggaatcac caaagcttaa ggaccaacca
      301 ctatagagtt cggtttctcc aactgcgagg gcaccaagtt tgaccttgaa ttttccgtca
      361 gtgaaggtat aggtaccttc taaggttaag gtgactgcgc ctgcttcgcc ggcaacttcg
      421 cctgctactg caagtggagc actgtcttta taccaaccat ctgctaatga gtcaccgcga
      481 agggcaaagt taatttcagt aataacaatc ttggttgtat cgaagtaaat tctataccaa
      541 ccttcgtgtc cttcagtaac tgtaatgttt tcaccagttt taatcgtttc aacttcttca
      601 acaagttctg ttggttgggt aaatcctgct ggtaagacgt tgtctgggtg tgaaccgaag
      661 tttagacccc agtcgttatt tgcacggagt ttgaagtcgc caactggaag tttcacaaat
      721 gggatgtaat agaatggtgc ttctaaatta tcatcattgt atgttaattc aatgtctaag
      781 tcaccatcat taccccagtt gtttaattca ccgacgacgc cccagttatc aattgcacct
      841 tcttcagggt tcggagttgc aggtgaacct tctggtaagt caccagtacg ggtaactctg
      901 agccattcat aagttgtttc aatttcgatt aagtaggcac cagcgttaac gatttggacg
      961 ttatcaaatc cgccagcaag gaagttttca tcgtttctgt cttcttcgcg gatttttccg
     1021 tatgagaatt gtaattcact atcccattct gcatttggaa tcttgatttt gaattcacca
     1081 cggaatccga taacgagttc gattgagtat aagttgctgt cttcagcttt tgttaagtag
     1141 gaacggacgt cccaatcatc accttcttca ccaactgcgt tccagttgat atagctgaag
     1201 tcagggttcg ctaatgaacc gacaacgtgg aatgtacgtg tgtctggtac atataatttt
     1261 tcatattttg gatagacatc agtatcttcc ataatgaagt ctgttggttt aaatggcttc
     1321 tttaaagctt catcgctata ccagccaagg aattcgtatc catcacgaac aggatcttct
     1381 ggcatctcga gtaagatatg tcctgctttt actttaattt cttcgtgaat gacagcaagt
     1441 tcattaacgt agaatgtaac gtaaacatct gaaccttccc aaagggctag aagtttaact
     1501 tcgctggtaa ctggggtatc ccagtcataa gcttcataat cttcaccaag ttgtaatcta
     1561 gcttctttat gccagccacg gaatgtgaaa tcatcgcctg gcttgaattc ggctggatct
     1621 gctggtttag tagccttatc gccttctcta atttggaggg tatcaatgta agtaccatca
     1681 tagtttttta agacgacact atgctttgct ctcgtatctt ctgatgaaga tgctccgcct
     1741 ttacatcctg cgagtaagcc tacggctaaa gcagtggtga aaagaccttt aaatagtttt
     1801 ttcattattt tctccttttc tatttttttg cctaaatggt ttctttggct tattcattat
     1861 acaacttttc acccttaaca gtcaagaata tgctaagggc taaagctgtt taataacata
     1921 gttataatgc ataatattcg aagtatttgc ctaaatgagt ggaaattgcc gaaatattag
     1981 ttataaacgg ctaattatac tgataatttc tttttgcgga agaataataa tattaagcca
     2041 acaccaacac caacaacggt gagtgatggg atagtaatat agagccaagt aagattagcc
     2101 tcttcccctt cttcacttgg aagcgtttca ctacttggta agtccggagt acttgttgcc
     2161 ggcggagtaa caccttcgcc agaaacagtc acgattgtcg cattgctact taaggaaaca
     2221 ctcttaccga gcactgacca tttttccatc gtgtttgtcg aatcaaagag tacatattgt
     2281 ccttctaaat cgtttatacg aacacgcgta cctgcaccta ggaagtaagc agtgtaagta
     2341 gtttcgccat caacgaccac tagtttgata agtgtatctt ggtacccgat ttctgaacca
     2401 aatttgacac taacattggc gctgatttcc gcacttgtgg tgtagtggaa tccaggggta
     2461 tcacgtttaa tggcaattaa ttctttatag cgttcaaaga catttaaatt agtaactttt
     2521 tcattccact taatgctatt aacactatac gggctttggt aagagttatg atcaaactta
     2581 ccgtcaccaa gtggtttaga acgtaagatt tcactaccag cgtgaataaa ggaaacacct
     2641 tgtgagagta gtgaaattgc ttcagcttgg atattttgtt gcgctagttg gtctagtcca
     2701 aataaatggc caccagcgtt gactaacttg tcatatagtg caaggttatc gtgacaagcc
     2761 acatagttta ctgtttgcac aggactactt tgtgtgccac taatcttgcc aagcatcccg
     2821 tcttttatac tattttttgt tccttgataa atttcactag cggcttgttg gacaaaaccg
     2881 cgtgctttac catttgtatc acctttaaca gagtcacgta atttatcatt aaatgctccg
     2941 actcctggaa ctttatgaat gttattttga ttagcgccat tagcacttac actaccgcca
     3001 cccatatccc acgcttcacc gataatgaag atgtcaggac gaatttccac gagacgatta
     3061 cgtactgtat tcattgattt aacagtgtgt aagcccatta agtcaaagcg gaagccaccg
     3121 attttatatt ctttcgtcca gaaagcagtg gaatcacgca tgaagttttc catcatgatg
     3181 cgttcacttg ccgtgtcatt accgcatcca ctagcgcttg ttggtgaacc gtcagcatta
     3241 taacggaaat agtagccagg cactaattta ttaaaggagt gaccaccaag gtttgcaacg
     3301 tgattataaa cgacatccat gataatacga atatcttcgg cagcgtaagc tttaacgact
     3361 tgtttaaatt cacgaatacg cacgagaccg tcatacgggt tacttgagta agaaccttct
     3421 aaaacatcat agttttgtgg gttataaccc cagttaaagg atggttcgcg ttcatcatta
     3481 gcttgatcgt agaacggaat gatttgtagg gcattaattc ctaattcttt aatgtgatca
     3541 aagccggttt tcactgtcac accaccagcg gtgtaagttg tgccttcttc gattaaacca
     3601 aggaagcgtt tgcgattttc ttcgctccca ttccaactct catccgcagt aaggtcggcg
     3661 acatggagtt cgtaaggaat aatttcggtg tgtgatttta ctgggtaatc aacttcatcc
     3721 caccctgtcg gatttgtttt actaaaatca acgatcatcc cgcggacacc attaatccca
     3781 gcacttttag cgtatggatc aattaattca acatagcctg ttgggtgttt agcgacaaaa
     3841 gtgtagtatt tgccgtgata atcacccgca agttcagcgc tccatacacc tttttcagtg
     3901 cgcacgaaat cgtgttctgt atatgtgtca ttaccaagat cagcatttag agcgaccggt
     3961 gtaccacttt cataaatccg taattttaat tcagaagcag ttggtgccca tgctttaaat
     4021 gttgatttag tgggtgaata agtaacccct aagtcttggc cattataagt catgttttca
     4081 ataaaataag gatcatcaaa taatcccgat aaaccaattt cactaatgac agcactgcct
     4141 gcggtaaaag cgacttcgac agtgtaagct ttactaaagt caggaaggaa tgctgcgcct
     4201 aaattaacgg taaacacata tgaccatgtg ttgttattat aagtatgctc tgccgctcca
     4261 cttgtgacat ttgtgccatc agccttaatc acaaaagttg ttggctcatt attagtggaa
     4321 acacgaactg tatttttact tgcaaagata ctttcagtta cttggtttgc ttttgcttct
     4381 tcagcactag caaatacagt aattaaaccg tttactaaat aaaagtgata ggcgttagta
     4441 acggtatcaa atgtaaaatc actatactga atatttacat caggtgtttg ccattcccag
     4501 cttccctttg tttttaagag gaagttaatt tgtgtttgat cggtaaaatt cgccactggc
     4561 acatgtaaat attttccgta tgcatcagtt tcggtgaagt tatattctga tccatcttca
     4621 tcttgacccc aaatccacat cgcataacgc gcataattag cgtcatttct aaaatagtga
     4681 aagacgatat gcggttcagc gtaagcctgc tctggcgtca ttactttttc cacttctaaa
     4741 gtaggttttg gacctactgc tcttttcggt gcacttttat cagtactgcc aaacaccatc
     4801 aaagaggcga gaagtattgt gaaaagtttt ttcataaatt tctctccttt cttaacgatt
     4861 atatttctca attaaatatg cgtataaaga actttcttca tcaaaagtct taaagtcttg
     4921 cattttccat tcccagtttg ggctaccaat tgtacttgga gaattcatct tggcttcatc
     4981 ccctaaacgc aataaatcct gcatttggaa aatagcgtaa cgcgcgtcac taccaaaatt
     5041 catcgccata aagttgtcgt agaaattatc aaattcatta aagcctaaat catctaataa
     5101 tttattcgct gctttcataa actttttatc tttccacttg aaatagccgc gaattggcga
     5161 gttatcatgt gtacctggat aaatacaacg attttctaac catgcatttt catcgcgctt
     5221 ttcaaaggga ttaaaagtga aagatgtaat ttccattcct ggtaaattat aatggtcacg
     5281 gagttctaat acttctgggc gtaagtcacc taagtcttca gcgataattt tgatatctgg
     5341 taattcttcg tatattttat caaagagtgc gtagcctggc ggataagccc agtgtccatc
     5401 attagcgcga ctgctaccgg cgggaatttt ccaataggaa tcaaaaccgc ggaagtggtc
     5461 aatgcggata atatcaaaaa gtctagcgtt ataacgcagt ctatcaaccc aaaattcaaa
     5521 gccggtttcc gctaagtagt cccagtcata aagcgggtta ccccacagtt gaccatcagc
     5581 gttaaagtag tcgcctggca caccaccaac gaatgtttgc tcatcatttt cgtcaagcaa
     5641 gaatgcttta cgattagtcc aaacgtcaac actatcaata cccacgtaaa tcgggatatc
     5701 gccaactatt tgcatcccgt aacgatgagc gtaagcgcgg agatccatcc attgtttata
     5761 agcaatgtat tgtaaccaca tttcaaattc aatggctttc gcaaatggtt ttaagttcac
     5821 accacggcgc gttttactcc attcacgttg cgcttctttc cagtaatgcc attgcactaa
     5881 gttgttatca cgtttaaagg taataaagat cgcgtaatct ttaacccatt tattatcttt
     5941 aacccatttt ttaaacccgc gtcggccttg ttttttaaaa ttattaaagg cgcgttttaa
     6001 atgacgcagt ttaaatttac gcactttgtc atagcgaact tttttactgc gcgcaaagta
     6061 gttaggaact tccggtagta atccttcttt tgttaaaagt tcgagactaa tataaatttc
     6121 atcaaatggt ttcgagccaa atggttgata aggcgaatta ccgtaaccaa gcgggttaac
     6181 aggtaagatt tgccataatt taaagccagt tttgctagct ttacgaataa aagtgcggcc
     6241 acttggacca aagtccccaa taccgtgttt gcccggtaaa ctactgaccg ctagtaaaat
     6301 gcctgcttga cgcattttat ttcctcctta atacgacact gctataaggg gctagtgtta
     6361 atgagccatc aacatacttt gcccttgtgc cggtagtgta aatgtcatga ataatttcta
     6421 cttcatattc actaatgaag cgtgtaaggt tcagcgaaac ttcttcggca agtaagttgt
     6481 gaattacatg cagttcacgc tggccattat gttcttttag tgttaaacca gtaacaccgt
     6541 tattattaag tcttaaatta taagcgcgag cgtttttaat ccatgggtga aagtttctta
     6601 cattaattac cttgcggtaa tggttaagta aactaaacgg ttcactaagt aaatcttctg
     6661 ccccgtactt tacttgattt gccatattgt aagtagtacc aattggtgga ttagtgcgat
     6721 gtttatcacc gactcttttc caaatcattg gtaagcggcg atttgcatca gtcatcgcgc
     6781 cttgtctact accttttaac ccaatttctt ccccataata cataaaggga acaccaggcg
     6841 ttaagatgta gacgcttgcg ccgagttttt gtcgttctaa ttgatcacct gtgaacattt
     6901 gactagaacg gtccatatca tggttagtta agaacattgc cattagtgca ttttcattaa
     6961 ttgcatatgc gtcactaaag gcgttgctaa tgtaatgcga aagttgcgga ccagagcgcg
     7021 ccataatgct gtcaattata aacccagtcg cgttagcgcc atcaaaatta aagagtgatt
     7081 caaccccggc gtagtaatac ttttgcacat taaagttttc ccatgcttca gcgacgatat
     7141 aactatcggg tttataactt ttaaccatat cgctaaacca ccctaaaaag tcgatgtttt
     7201 ccggatgagt ttcaaagaaa tgacccgcag cgtcaagtct aaacccttta accccgcgat
     7261 caagccaaaa tttactaatg ctcgtaattt cttcacgtac ggctgtattg ttgagtagta
     7321 gttcaggcat atggtcgcca aagtaaccaa ttttaacttt tacttcgcca ttttcaatgt
     7381 aaaagtcata ccagttacct ttatcatttg ggtcgttagg attgtattct ggtgtattaa
     7441 accgtcttct cccctcaaga aaccacgggt gcgtgcgcgc agtgtgatta ataacgaggt
     7501 caataataac atcaatatta tgttctgctg cgacactgat taaagtatca aagtcatcaa
     7561 gcgtaccaaa gtcaggatga atcgctttat aatcggttac atcatagcca tgataactag
     7621 gtgaagggtt aatcggggtt aaccaaatcc ccttcactcc tagttcttgt aaatatggaa
     7681 ttttatcagt gatacctttt aagtcgccat gaccatccgc atttgtgtca gcgaatgagc
     7741 gcacgaaaat ttgatagtat acgccgcctt tttcattagt gattgactca tcaatttcgt
     7801 aatccgtcgg cgcttgtagt atttcttcac tcgaaacact ttcgcttggt aaatctttac
     7861 aacttgccat aaatagtaag gggaagataa gcattaaagc acgaaggctt tgttttgctt
     7921 tcatggttat cccttaactg ctccacctgt tacaccgctg acataatagc gttgcatgaa
     7981 gacgaagaga attgtaattg gaattgaaat gagtactgct cctgcacaga agagcgtgaa
     8041 gtacttacca agtttttcta gaccgccatt aatcatgtca taaagtccgc gggcaatgtt
     8101 ccacgcttca cgtttaccac gtgtaatcgt ggcaacgaag atatagtcac cccacgggcc
     8161 cataaatgca cctaaggcgg tgtaaattag aattggtttt gaaagtggca aaataatatg
     8221 ccagaagatt tgtgctttat tagcaccgtc aatttccgct gcttcatcga ttgatttact
     8281 aatcgtatca aagtagcctt tagagacgag ataccccatc gctgctccgc ttgtaccaac
     8341 aaggataagt gagatgaggt aattagaaac aggaacttct ggtcgtgttt cggttggtaa
     8401 taatccaatc gcttttaaga tgtggaaaat tgcaatcatt gacatgaagc cagggaacat
     8461 tccgagaatc attaaaaatt tcataagacc tttgcgcgct ttaaaacgaa gccggctaaa
     8521 ggtataactt gtaagtaaga caatagttgt ggaaatgata ctactaaatg tcgccacaat
     8581 tagcgtattt gtaaaccatt ttaagtacgg catttctgct gtagtgaaaa gtcgtctaaa
     8641 gttatcaaag ccgatttctc gcgggaaaag gtaagaaaca ggtccagttg actcaatgcg
     8701 gagtgcgtta gcgattaacc aaataatcgg gaaaatccac gccacacaca ttgccgctag
     8761 taagacataa aggaaagcat cgacaacacc gcgtatcaca cgttgatttt gccaaaactt
     8821 aactttaccc tttggaattc gtgaggaggc cgcactagcg tgtaagcctg ttggttgttc
     8881 agtaacaaca tcttttttaa catttttact catattatgc aaagtctcct tccgattgtg
     8941 ctgcggatgt ccgcgaatag agcactaaag taatagtcgc actgataagg aagatgaaga
     9001 tccctagtgc gctaccaatc gcgtactctt catgtgctcc tccaacggta agcgcgtata
     9061 gccacgtaat taaaatatca gtgtgaccat acggtacgcc aggaatacca gtacttggtc
     9121 ccccacctgt caggaagaat ataacgttaa agttattaat gttcccaatg aattgggtaa
     9181 ttaagtacgg acctgtaaca aacaagatgt aaggcatcgt aatttggaag aattgtcgga
     9241 caggtccagc gccgtcaatt ttcgctgatt catataattc agtcggaata ttcattaaga
     9301 tacccgacgt taatagcatt gtgtaaggaa ccccaaccca aatatttacg acaatgaccg
     9361 taattttagc taggactgga tcatcgagga atttaatccg ttgcctaatc catccccact
     9421 gtaatagtgt ttcattaagc ggtccgtatt ctttaagtaa ttggttcatt aagagcaagg
     9481 taacaaattg gggaacggca atggaaataa caaagatggt tcgccacagt ttctttaatt
     9541 taatgctctt gcgattaata atcattgcga gaattaatcc gaaaatatag ttagtaaaag
     9601 tcgcaaagaa tgcccaggta aatgtccatt tcaagattct taccatcgca ctaccaaatg
     9661 tcgcattagt agcggaagcg ccttttgtac taaataaatt tttaaatgtg tctaacccaa
     9721 cccaagtaaa taatttattc ggcggggcag tgtcgcggtt atagttagta aacgcgagca
     9781 aaatcataaa gacaagcggt aatatcgtaa acatcgttaa tgttattgcc ggaagtgaga
     9841 gcgtcgtgaa gtggaacctt tcatttaata atgacttaag ttcatcagta aattttggaa
     9901 gtgggttctt aaatgcttct tcactacctt cacttttcgc aagttcatat tttgcttttg
     9961 cttcgttata agctttttct gctttaaaag tgccgcgaac ttgaacaaag tacataacga
    10021 tgaaaaagac ggtaattaaa attgttgcga ccgagaataa cataattaag agcgagttat
    10081 ctttaaagat aggtttctca tatgtaataa aatctgggtc acaatcttca ataggagcac
    10141 ctggtggcca agtacattcc gccatatatt caaccgcaac ttctccgagt gttctaaatt
    10201 taattaacca tgctagtcct cctcctggga cgaacatgaa aatgaaatac gctacttgga
    10261 ttaaaagata aataatgcct tttattggac ggcccattga catttgtcca cttcccatta
    10321 tgaagtgcga gagtttagtg cgccatcctc ctttgacaaa gtgtgtgtaa actgatacaa
    10381 aaaagttttt aatcattttc cataaattaa cgaaagtgtt tttaatggca attcctgtat
    10441 caataaaaaa attgacgatt cctttgccta aacgcacaaa gaaaccacca attgtggaca
    10501 agaaatcttt aaaccaagtg ccagccgtac gtgagtgggc cgatttgact tttttatcac
    10561 ttgtttccat attttatggc ctcatttcta gaaagatagg agagccagcg atgaactggc
    10621 tcccaaaatt aattaaaaat taatcgatta tatgtgttta ataattttat attattttgt
    10681 taaggcatcc acaaaggcat caagtgttgc ttgaagtgtt tgtggtgatt cttcacttgc
    10741 tggacccttc cataagaagt taccaacgat agcagcattg tcccagaatt tggagccaac
    10801 tgattttgct tgtgaaatag catatggcgc ttgggcacca agtgctttaa gggtaacatg
    10861 gtcggctaag agttcttcgt ccgcaagtaa gtttttgttt gatggacccc atccgtttgt
    10921 ttcaaaacga tagcgttggg cggcttcgtc agtaatgtag tcggcaaagg caagtgccca
    10981 tccgaggtta ttactagaag atttaacacc aactaatttg gaaccagcaa aggaacccca
    11041 ttgtacggtt gttccgtttg cactcttaaa tgttgggagc ttagtggcac caaggttttc
    11101 acctaatttt tcttttaaga gttcggtatt ccacacacca gtaacagcag cgataacttt
    11161 gttaccattg gcatcactta agtcgtcaag tgcagcaccg ttaaagtcaa ctgatttaaa
    11221 tccgttgtct actcttggtt ttaagagatt gctaacacct tgtaaggcgt caactgcttc
    11281 agcactgttg aagttagtgg tatgaagtct tgtaattgga ttataagtga tatcacagag
    11341 atattgaatg aaggaagttg cataataccc attacccata tcaacgacaa attgatgttc
    11401 ggcagtagtt ttcgccataa ttccttctaa agtttcgacg tcggtttctg ttaagagtgc
    11461 cttattgtaa ataagcatat agccgttgtc atttgtaaca gggaatgcat aaagttcatc
    11521 gtccactgct gaacttgcgt tttcggcagc actagcaacg tcacgctctt taactttatt
    11581 cgcataacct tttggtaatt ttgcgataac ttcacgagct tgcattgttg gaagttggtc
    11641 atctgcgaag aaaaatacgt cagcagcagt acttacgtct tgaaggacgt ttgttgcggc
    11701 atcagcttct ccaacgtcag cgtaactaat tgagaagtta atttctggat gcttctcttg
    11761 gaattcagta gcgtgtttgt gatagagtgg ttcttcagca cttgagcccc acactgatag
    11821 acggacacgc tcctttgatc cgcctccact cgttgttgtt gtttggcttg ttgttccttg
    11881 actaacatca ccactgacat cagcgctact gccaccatta cagccggcaa gtaaaagcaa
    11941 acctgcaaca agtaaagcga tacgtttttg tttaaacata aattct
//
LOCUS       DAOSTA010000031        11686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_5466_length_11686_cov_6.925888, whole genome shotgun sequence.
ACCESSION   DAOSTA010000031 DAOSTA010000000
VERSION     DAOSTA010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 11686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11686
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5466_length_11686_cov_6.925888"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLU08_03740"
     CDS             complement(<1..575)
                     /locus_tag="PLU08_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ70836.1"
                     /translation="MEKNNKERVTLKDIAERAGVTVNSVSRALRGHKSISEPTKERVR
                     AIAKELGYIPDAQARLMRGAKLRVIAVVYDNIVNPYYAIMNNHLNNELTKANYRPMVF
                     FESSQDGSLSLEVAREIISMRIVGVITYLVPSKEVIELFEDHNVPIVLLGGTGDALGI
                     DSVASNDYRGGKLAGYKLFDLGGKNFAYIGVT"
     gene            711..1958
                     /locus_tag="PLU08_03745"
     CDS             711..1958
                     /locus_tag="PLU08_03745"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOQ70837.1"
                     /translation="MKIKRHVRHLMVLVFMMVISSCALFPTSSEKDSLPIASSSSETF
                     SSANESSEELSTSEVPSSESEVQSSEPEPSSKELSSSEEPSISVELEMKRSTYTNSVY
                     FYDFPDPSGVYDPIQRMWFLYATGGQYLRSSDGVTWGQKGWVFGHLPDWGTKGAGLWA
                     PDVQYINGQYVMYYSLSTWGDPNPGIGYATAPKPNGPWTDHGKLFLSQEIGVNNSIDA
                     NAFVDPNTNRVYLVWGSMRGNYLVELTRDGLGFKAGSVEQAALSKIRVAGLDTVHGWD
                     AETYEAAYIRYWDGYYYLFLSMGTCCEGLSSTYKVIVGRALSPYGPYYDDTGTDMRNR
                     SAGKLVLQKNDTFVGTGHNSVIDDVNGEPWIYYHAYHVDKQSARVLLQDKLLFDENGW
                     PYVEGFTPSTTEQIGPYYHSVVE"
     gene            1972..3111
                     /locus_tag="PLU08_03750"
     CDS             1972..3111
                     /locus_tag="PLU08_03750"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOQ70838.1"
                     /translation="MKRYRILLPLLCVALLVSCNQTSSREEPITGSEVVSEISDDSLS
                     ETIASSEKPRELTTVFFQNPIYNADFPDPSIVRHTDGQFYTFATGGRIIKSANLVEWE
                     ALSNAIARPTWGTPGANIWAPDVQYINGQYVMYYSLSRWDDPNPGIGIATATHPAGPW
                     TDHGKFFLSQEIGVNNSIDPMVFVDEDERVYMFWGSFRGIYAIELTADGLGFKDGSVE
                     QAANNKVHIAGFETNRNFDISTFEGVYIIKKDNYYYMFLSTGTCCSGDYTYNVVVGRS
                     ESILGEYFDSYNRSMKNANVGHSVVHQNDFFIGVGHNSILRDDAGTYWILYHGFDATV
                     ETRNARKLLIDKLIWNADNWPSTLGNVPSNNYRPGPELYLSDENN"
     gene            3343..5361
                     /locus_tag="PLU08_03755"
     CDS             3343..5361
                     /locus_tag="PLU08_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOQ70839.1"
                     /translation="MKKHRYLVLALLSLVLLGSCKGDGSESGKTSEPQVSTTSTVGSE
                     EGERTITWYGTDDIVITIGDEFTLLDGVRAVDSVDGELEVVVADDDYFDCYYVSQYTI
                     KYEATNSVGTKSTKYRMVQVVKGCNVFNGNFAMGKTYWRFDTPGGQGTFNVINEEAVF
                     SFTDTGSEAWSLQLYQQPGITFSAGRTYEMTFIAKSDKGRSISAGFENYNGFAMLIPG
                     YPAMVLTSEFQTYSAIYTADADYPSVKPVLYLGRGLDIDGTASKANPLDLVVDDIKVR
                     EIVLPEESKRPVFSNAGPVTVFTGDQFEALPPVTAVDYKGNDISDRIERIGAIPDSIA
                     AVTRMLVSYRVTDTEGNFNFINRSVSYQIARDNPYNLINGDFNNGLQGWVRDVNQTNG
                     TGAANFIDNEDGTITIDITNGSNDNWHIQFYQSNVSIQAGKIYRVTLIAKASIERKAT
                     IEISNPSQSYSRVATKLISLTTEYQTFLLEFKSTVTCLAKVSLLLGAQGANQVTLDKY
                     DNDMINADEATTIDLRDYEDYEVINGDFRYGYYGWTQEMTHGAEVDFIADQANEQIKI
                     DVISPGTANWHVQINQDGKTFTEGKTYIMKVSASALAATTIAMEVTNNNGETVLARED
                     ITLSATLTEYTMEFTPSQTFTLGKCALLLGTSEVTVITVKSVAIGIKP"
     gene            5361..6506
                     /locus_tag="PLU08_03760"
     CDS             5361..6506
                     /locus_tag="PLU08_03760"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOQ70840.1"
                     /translation="MKRKIGFTLILCTLLVGCTPPVSSETLPPTSEDVVTSQSLEEVP
                     TTYENPVWEPVLADPAVIRADDGTYYAYGTQDNALWGDYFGVRYTPILSSTNLVDWEY
                     RDQAFKPATMPTWSETSGAGLWAPDIVKIGNTYNLYYSLSAWGDPNPGIGVATGPSPL
                     GPFTDQGKIFDSNEIGVYNSIDQAVFVDTDNRVYMFWGSFSGIYAVELTADGLSVKGT
                     IAEANANKVLVAGVDNKRWDIGNYEGAYIIRERGYYYMFLSTGTCCEGHHSTYEVRVC
                     RSESILGPYYDKAGNLMTYRQNLGTLVVRGNDDFAGVGHNSIVKDDAGESYIVYHGFD
                     KFEDPYYGTTNRRALLIDKLVWDKEYWPSVEGLGPSRGAERPYIAPR"
     gene            6535..7860
                     /locus_tag="PLU08_03765"
     CDS             6535..7860
                     /locus_tag="PLU08_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ70841.1"
                     /translation="MKKKTIPLLLLALVLTGCGQTSEKPSQPGGGGEGSVEVSLPTFE
                     NVALTYGNPITGADGTAMRRLVREFNEEYQGQITVTESFMPEVDFYESLVATIPMKRS
                     FDIALIHSYRVPSFANKNLLFPLDNLTASTGVDITRENYIEDVYDAMIWDNKQYGVPL
                     DVHSIMLYYNKDLLAQHGCEVPTNRAELIAAAKKMPQTNAGGWGLPLSTTWPSEYIFT
                     TALYQNGGKEIDKNTLMPAFNNTAGEKALKAVADLIHIEKISPLNVNVDADLLMFQQG
                     KAMFHINGNWMLNSLVDSGINFGVTSLADMFVDTPTADSKKVASRSHTFVLPQGRNET
                     IKQQAALVFIKYVTENAYLWAESGGHIPASNIARNTEEYLALPYHQTYGDVNMYTLNE
                     PSPYYYEAFAPVFSRVTTAMSDATYDAMALLNAAAEEGEQAVLIAQLEG"
     gene            7880..8851
                     /locus_tag="PLU08_03770"
     CDS             7880..8851
                     /locus_tag="PLU08_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ70842.1"
                     /translation="MDAKQKLKLKEGIKRYLTVAFFLLPYIALFATFFIFPLFFGVYI
                     SFFRYNVFDPSASKFIGFDNYKLAISGLTIGTTNVTYYAKMFWGSLKNTLLFVCVSVP
                     LLIIIPIFIAILIDIQPRFYKVFRVIFFLPTILSISAVGIIFKWQFDTQYGFVNGLIR
                     VFGGKEVAWLNSQPYAWIAIVVTTIWWTIGTNTVIIGAGLKEVDKQMYEAAEVDGASY
                     PRVIWSIALPSIKNQIFIIVFTTIIASFNIYGQPDILTGGGPAPSGTVAAGTPGTTTV
                     VMMVIRGLMTGVNRQPGLAAAMSLIFGSFILVVSLGQAAYNKYREKY"
     gene            8851..9717
                     /locus_tag="PLU08_03775"
     CDS             8851..9717
                     /locus_tag="PLU08_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ70843.1"
                     /translation="MAIKSGVKRRKRVAFIILLVLSILWVLPLLWALGTSFKSANDMR
                     TNVITIIPREFTFGNYKNLFDNSAVYPIFKWLRNSALIAIIHTLLYLIIASMAAYAFG
                     VMRWKGRNIVFGFLLATMAIPTVINLTPLFTMVIRFGWLDSEAAWKSIFALIVPGLGG
                     TFGLFIMRQFFMNIPYDLVESAQIEGLGHFGVYRKIILPLGKSAVMVAGLFAFMGSWN
                     DYLWPTLTAAVHFDQNWYTLQTGLATMQSQSNYDPGQVMAAAIISIIPVLIVYMFVQD
                     KIIEGVSHTGIK"
     gene            9735..11471
                     /locus_tag="PLU08_03780"
     CDS             9735..11471
                     /locus_tag="PLU08_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012863473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ70844.1"
                     /translation="MYRKEYPRPALVRKEWLNLNGIWDFAFDDDNVGLNENWVEKGKF
                     PLKINVPFAFQTPLSGINDPSFHDYVWYRRFFSVKKEEGKRFILHFGAVDYECKIYLN
                     KKHIGDHIGGQVPFSFDVTDFLNFNEEELVVWVSDPSTELSMPRGKQYWKEKSEVIWY
                     NRTTGIWQTVWLEIVPEAYVKSMRITPLFDEGKVHFSLSFSQVGRELILEDENGKIKY
                     AKVPNKLTHEFTVQTFLDEQDFITKAWSPENPHLFPFKLRYGDDLISSYFGMRKVHAD
                     KGITYLNNKPYYMKLVLDQGYWPQSLVTPPSKEALEYDIKMAKAMGFNGARKHQKCED
                     PYFAYYADKLGFLVWGEMASSIRFSEKSAWRDKHEWPQIIARDYNHPSIVAWVPLNES
                     WGVDDIGHDKRQQQHALELYYLIKSLDNTRFVVGNDGWEQIITDICAIHNYNHGRHDD
                     KDAIAAFNKSISTKEELLKSRPANRDIYAEGYSYRGEPILLTEFGGISYQMNEDKGWG
                     YTAVGDAEHLLSEYKRIILAIKESTALVGYCYTQLTDVEQEINGLLTYDRKPKLPVEK
                     VKEVNDLIQKYK"
BASE COUNT         3629 a         1969 c         2540 g         3548 t
ORIGIN      
        1 gtcacaccaa tgtaggcaaa gtttttacca cctaaatcaa ataatttata acctgcgagt
       61 ttaccaccac gataatcgtt tgacgcaacg ctatcaatcc cgagtgcatc cccagtgcct
      121 cctaggagta caatggggac attgtggtct tcaaaaagct caataacttc tttacttggc
      181 actaaataag taataacacc gacaatgcgc attgagatga tttcacgggc aacttcaagt
      241 gataaagaac cgtcttgact actttcaaaa aataccattg gccggtaatt ggcctttgtt
      301 aattcattat taagatgatt attcataatt gcatagtaag gattaacaat attatcgtag
      361 actacagcaa taactcttaa ttttgcccca cgcatcagcc tagcttgcgc atcagggata
      421 tagcctaatt ctttagcaat cgctcttact ctttcttttg tcggttcaga aatactttta
      481 tgaccacgta atgcacgtga aacactatta acagttacac ctgcacgttc cgcaatatct
      541 tttaatgtta cgcgctcttt attattcttt tccataaaat aattatggca tatattcggt
      601 gcaaaataaa ttagattaac aattagtgcg acgctttgtt tacaaaaaac ttttttcgtt
      661 ttataattag attaacgata acctttgaaa aactataata gggggcgctt atgaaaataa
      721 aacgacatgt tcgccattta atggtcttag tatttatgat ggtaatttct agttgtgcac
      781 ttttccctac aagtagtgaa aaagattcgc tcccaattgc ttcaagtagt agtgaaacgt
      841 tttcgagtgc aaatgaaagt agtgaagagc tatcaacaag cgaagtaccc tcaagtgaaa
      901 gcgaagtgca aagcagtgaa cctgaaccaa gtagtaaaga gttatcaagt agtgaggagc
      961 ctagtattag cgtggaactt gaaatgaaaa gaagtacata cacgaactct gtgtattttt
     1021 atgatttccc tgacccttca ggagtatatg acccaattca acgcatgtgg tttttatatg
     1081 caacaggtgg ccaatattta cgctcgagcg atggcgtaac ttggggtcaa aaaggttggg
     1141 tctttggtca tttaccagat tgggggacaa aaggagcagg actttgggca ccagatgtgc
     1201 aatatataaa cggacaatat gtaatgtatt actcactttc aacatggggc gatcctaatc
     1261 cggggattgg ttacgctact gcgccaaaac caaatgggcc gtggactgat cacggtaaat
     1321 tatttttatc acaagaaatt ggtgtgaata atagtattga cgcaaatgct tttgttgatc
     1381 ctaacactaa ccgtgtttat ttagtgtggg gttcaatgcg tggtaactat ttagtggagc
     1441 ttacgcgtga cggtcttggg tttaaagcgg gaagcgttga acaggcggca cttagtaaaa
     1501 taagagtcgc gggcttagat acggtgcacg gctgggacgc tgaaacttac gaagcagcct
     1561 atattcgtta ttgggatggc tactattatt tattcttatc aatgggtaca tgttgtgaag
     1621 gacttagctc tacttacaaa gtaatagtcg ggcgcgccct ttcaccatat ggtccttatt
     1681 atgatgatac aggcacggat atgcgcaatc gttcagcggg caaactagta ttacagaaaa
     1741 atgatacatt tgtcggcact ggtcataact cagtaattga tgatgttaac ggcgaaccgt
     1801 ggatttacta tcacgcttat catgttgaca aacaaagtgc gcgagtttta ttacaagaca
     1861 aattattatt tgatgaaaat ggatggccat atgttgaagg ttttacgcct tcgacgacgg
     1921 agcaaattgg accatactac catagtgtag tagaatagaa gggggaagag tatgaaacgc
     1981 tatcggatat tgttgccgct actttgtgta gcgctacttg ttagttgtaa ccaaacttct
     2041 agcagggaag aaccgattac tggaagtgaa gttgtaagtg aaataagtga tgattcactt
     2101 agtgaaacga ttgcttcaag tgaaaaaccg cgtgaactta caacagtctt tttccaaaat
     2161 ccaatttata atgctgattt tcctgatccg agtattgtcc gtcacacaga tggacaattt
     2221 tatacatttg ccactggtgg acgcattatt aaaagcgcta atctagtgga atgggaagca
     2281 ctaagtaatg ccatagcgcg tccaacttgg ggcacacctg gtgcaaatat ctgggcacct
     2341 gatgtccaat acatcaacgg acaatatgta atgtattatt cattatcaag gtgggatgat
     2401 cctaaccccg gaattgggat agcgactgcg acgcacccag caggaccctg gactgatcac
     2461 ggtaaattct ttttatcaca agaaattggt gtaaataata gtattgaccc aatggtgttt
     2521 gtcgatgaag atgaacgtgt ttatatgttt tggggtagtt ttagaggtat atacgcaatt
     2581 gaattaacag cggatggcct tggttttaaa gacggaagtg ttgaacaagc agcgaataat
     2641 aaggtgcata tcgctggttt tgaaacaaat cgtaactttg atatatcgac cttcgaaggt
     2701 gtatacatta ttaaaaaaga taattactac tatatgtttt tatcgactgg tacatgttgt
     2761 tctggtgatt atacttataa tgttgtcgtt gggcgaagtg aaagtatttt aggggaatac
     2821 tttgattcat ataatcgtag tatgaaaaac gctaatgttg gacattccgt cgtccatcaa
     2881 aatgatttct ttattggtgt tggtcataat tcaatcttgc gtgatgatgc tggaacatat
     2941 tggattcttt atcacggatt tgatgcaaca gtggaaacgc gtaatgcccg caagttatta
     3001 attgataaac tcatatggaa tgctgataat tggccgagta cattaggtaa tgtgcctagt
     3061 aataattatc ggccaggacc tgaattatac ttaagtgatg aaaataatta atgacgaatt
     3121 aattagtttg tccttgacta tttttgctac gctttataaa ataaaggtgt aagtaagatt
     3181 aacgataatc taaaaaacaa aatcatacca ataattcaat aaaaatagat cgaaattagt
     3241 gccccacaac ttaaacttca gaaactacca tgatagtttc gtatccttat gtttaattaa
     3301 ataattgatg gtaaataaat caaaggaaaa ggagacttga ttatgaaaaa acatcgctat
     3361 ctagtgttgg cactactttc attagtgtta cttggctctt gtaaaggtga tggtagtgaa
     3421 agcggaaaga caagcgaacc gcaagtaagt acaacatcta cagttggttc agaagaaggt
     3481 gaacgaacaa ttacgtggta cggcactgat gatattgtaa ttacgattgg agacgaattt
     3541 acattacttg atggggttcg cgccgtcgac agtgttgatg gtgaactaga agttgtcgtc
     3601 gctgatgatg actattttga ttgttactat gtatcgcaat acacaattaa atacgaagcg
     3661 acaaatagtg ttggcactaa aagtacaaag tatcggatgg tgcaagttgt caaaggctgt
     3721 aatgtgttta acggtaactt tgcgatgggt aaaacttatt ggcgttttga tacaccaggt
     3781 gggcagggga catttaatgt cattaacgaa gaggccgtct ttagctttac cgatacaggg
     3841 agcgaagcgt ggtccttaca gctttaccaa caaccgggga ttacctttag tgctgggcgt
     3901 acttacgaaa tgacttttat cgctaaaagt gataaagggc gttcaatttc tgccgggttt
     3961 gaaaattata acggttttgc tatgttaatt cctggttatc cagcaatggt actaactagt
     4021 gaattccaaa cttattcagc tatttacacg gcagatgctg attatccgtc agttaaacca
     4081 gtgctttatt taggacgcgg tttagatatt gatggtacag caagtaaggc aaatccactt
     4141 gatttagttg tcgatgatat taaggttaga gaaattgttt taccggaaga aagtaaacgt
     4201 ccagtgttta gtaatgctgg accagttact gtattcactg gtgaccaatt tgaagcatta
     4261 ccgccagtta ccgctgtgga ttataaaggt aacgacatta gtgaccgcat tgaaagaatt
     4321 ggtgcaattc ctgattcaat cgcggctgtc acacggatgc ttgttagtta tcgggttacg
     4381 gacaccgaag gtaattttaa ctttattaat cgctctgtta gttatcaaat tgcgcgtgat
     4441 aacccgtata acttaattaa tggtgacttt aataatggac tccaagggtg ggtacgagat
     4501 gttaatcaaa ctaatggcac tggtgccgca aactttattg ataatgaaga cggtacgata
     4561 acgattgata taacaaatgg tagtaatgat aactggcaca ttcagtttta tcaaagcaat
     4621 gtgagtattc aagcaggaaa gatttatcgt gtaactttaa ttgctaaagc tagtattgaa
     4681 cgtaaggcca caattgaaat tagtaatcct tcccaaagtt attcacgagt cgcaactaaa
     4741 ctaatttcgt taacgacgga atatcaaaca ttcttacttg aatttaagtc aactgttact
     4801 tgtttagcga aagttagcct tttacttggg gcgcaaggcg caaatcaagt cacacttgat
     4861 aaatatgata atgatatgat taatgctgac gaagcaacaa caattgatct tcgtgattat
     4921 gaagattatg aagtaattaa tggtgacttt agatatggtt actatggctg gacacaagag
     4981 atgacgcatg gtgcggaagt tgatttcatc gctgatcaag cgaatgagca aattaagatt
     5041 gatgtcatta gtccaggaac ggcaaattgg catgtgcaaa ttaatcaaga tgggaaaaca
     5101 tttacggaag gcaagactta cattatgaaa gttagcgcaa gtgcattagc ggcaacgacg
     5161 attgcgatgg aagtaacgaa taataatggt gaaacagttt tagcccgtga agatattact
     5221 ttaagtgcaa ccttaacaga gtacacgatg gaatttacac catcacaaac atttacactc
     5281 ggaaaatgtg cgctcttact tggcactagc gaagtcacag taattacagt aaaaagcgta
     5341 gcgataggaa ttaaaccata atgaaaagaa agataggatt tactcttatt ttatgcacct
     5401 tgctagtggg atgtactcct ccagtttcta gtgaaacact acctcccact agtgaagatg
     5461 tagtaactag ccaaagtttg gaagaagtac ccacaactta tgaaaatcca gtgtgggaac
     5521 ccgttttagc tgaccctgct gtgattcgcg cggatgatgg gacttattat gcctatggta
     5581 cgcaagataa tgcgttatgg ggtgattact ttggtgttcg gtatacacca atcttaagta
     5641 gcaccaatct tgttgattgg gaatatcgtg atcaagcctt taaacccgca acaatgccaa
     5701 catggagtga aacttcaggt gcggggttat gggcaccaga tatcgttaaa attggtaata
     5761 catataactt atattattcg ctttcagcat ggggcgatcc taatcctggg attggtgtag
     5821 caacagggcc ttcaccctta ggcccattta ctgaccaagg caaaatcttt gatagtaatg
     5881 aaattggtgt ttataattca attgaccaag cagtatttgt tgatacagat aatcgtgttt
     5941 acatgttttg gggcagtttt agcggcattt acgctgtgga attaactgct gatgggctta
     6001 gcgtcaaagg tacaatcgct gaagcaaacg ctaataaagt acttgtcgct ggtgttgata
     6061 acaagcgatg ggacattgga aattacgaag gtgcttatat cattcgtgag cgcggttatt
     6121 actatatgtt cctttcaacg gggacatgtt gcgaaggtca ccactcaact tatgaagtgc
     6181 gtgtgtgtcg cagtgaaagt atcttaggac cttattacga caaagctggg aacttaatga
     6241 cttatcggca aaatctaggc acgctagttg tgcgtggtaa tgatgacttt gctggggttg
     6301 ggcataactc aattgtcaaa gatgacgctg gtgaaagtta tattgtttat cacggttttg
     6361 ataaatttga agatccttat tacgggacta caaatcgccg tgcattatta attgataaat
     6421 tagtgtggga caaggaatat tggccaagtg ttgaaggctt aggaccttca cgtggggccg
     6481 agagacctta tattgcacca cgctaacaat atacatttag gaaaggtaaa aattatgaaa
     6541 aagaaaacga ttccattatt actacttgct ctcgtattaa cgggctgcgg acaaacaagt
     6601 gaaaaaccta gccaacccgg tggaggtggt gaaggtagtg ttgaagtatc attaccgaca
     6661 tttgagaatg tcgccttaac ttacggtaac ccaattacgg gtgcagatgg aacagcaatg
     6721 cgtcgccttg tccgtgaatt taatgaagaa tatcaaggac aaattacggt gactgaatca
     6781 tttatgccgg aagtagattt ctatgaatca cttgttgcga caattccaat gaaacgttca
     6841 tttgatattg ctttaattca tagttatcgt gtaccatcat tcgctaataa aaacttatta
     6901 ttcccgctcg ataatttaac agcatcgacg ggagttgata taacgcgtga aaattatatt
     6961 gaagatgtgt atgatgcgat gatttgggac aataagcaat atggtgtccc gcttgatgta
     7021 catagcatta tgctttatta caataaagat ttacttgcgc aacatggctg cgaagtcccg
     7081 actaatcgcg cagaattaat cgctgcggcg aaaaagatgc cacaaactaa tgctggtggc
     7141 tggggcttac cgctttcaac aacatggcca agtgaatata tttttacaac agctttatat
     7201 caaaatggcg ggaaagaaat tgataaaaat actttaatgc cagcctttaa taacactgct
     7261 ggggaaaaag cactgaaagc agtggcggat ttaattcata ttgaaaagat ttcaccactt
     7321 aatgtgaatg ttgatgctga tttactcatg ttccaacaag ggaaagcaat gttccacatt
     7381 aacgggaact ggatgcttaa tagtcttgtt gattcaggaa ttaattttgg tgttacttcc
     7441 ttagcggata tgtttgttga tacaccaact gcggatagca aaaaagttgc aagccgtagt
     7501 cacacctttg ttttaccaca agggcgaaat gaaacaatca agcaacaagc tgcattagtg
     7561 tttattaaat atgttactga aaatgcatat ttatgggcag aaagtggcgg acatattccc
     7621 gcaagtaata ttgcgcgtaa taccgaggaa tatttagcgc tcccatatca tcaaacttat
     7681 ggtgatgtta atatgtatac acttaatgaa ccttctccat attattacga agcttttgca
     7741 ccagtcttta gccgtgtaac aacggccatg agtgacgcta catatgatgc gatggcttta
     7801 cttaacgctg cggcggaaga aggtgaacaa gccgtcttaa ttgcgcaact tgaaggttaa
     7861 aggaaagttg atataaatca tggatgccaa acaaaaatta aagctgaaag aaggaattaa
     7921 acgctactta acagtggcct tcttcctatt gccttatatc gccttatttg cgacattctt
     7981 tatctttccg cttttctttg gtgtttatat ttcctttttc cgctataacg tctttgatcc
     8041 aagtgctagt aagtttattg gctttgataa ctacaaatta gcgatctcag gcttaacaat
     8101 tggtacaacg aatgtaacgt attacgcaaa gatgttttgg ggttcactaa agaacacgct
     8161 acttttcgtc tgtgtttctg ttcccttatt aattattatt ccgatcttta ttgcaatctt
     8221 aattgatatc caaccacgct tttacaaagt atttcgagta atctttttct taccgacgat
     8281 tctttcaatt tccgcggttg ggatcatttt caaatggcaa tttgatacgc aatatggctt
     8341 tgtaaatggg ttaattcgcg tgttcggtgg taaagaagtt gcatggctta attcgcaacc
     8401 ttatgcatgg attgcgattg tagtaacgac aatttggtgg acaattggga caaatacagt
     8461 cattattggc gccggcttaa aagaagtcga taaacagatg tacgaagctg cggaagttga
     8521 tggcgctagt tatccgcggg tgatttggag tattgctttg cctagtatta aaaatcaaat
     8581 ctttatcatt gtgtttacga caattatcgc tagttttaat atttatggtc agcccgatat
     8641 cttaacgggt ggtggaccag caccaagcgg gactgtcgcg gctggtacac caggcacgac
     8701 aactgttgta atgatggtaa ttcgcgggtt aatgactggt gttaatcgtc aacctggttt
     8761 agcagcggcg atgtcactaa tttttggttc atttatctta gttgttagtc ttgggcaagc
     8821 tgcatacaac aaatataggg agaaatacta atggcaatta aaagtggagt gaagcgccgg
     8881 aaacgtgtcg catttattat tctcttagtg ctgagcatct tatgggtact accgctttta
     8941 tgggcgcttg gtacttcgtt taaaagcgca aacgatatgc gcacgaatgt aattacgatt
     9001 attccgcgtg aatttacctt tggtaattat aaaaatttat ttgataatag cgcggtatat
     9061 cctattttta aatggttacg caatagtgcg cttattgcga taattcatac gcttttatat
     9121 ttaattattg cttccatggc agcctatgct tttggggtaa tgcgctggaa gggtcgtaat
     9181 attgtttttg gttttttact agcgacaatg gcgattccga cggtgattaa cttaacgccg
     9241 ctatttacga tggtcattcg ttttggctgg cttgatagtg aagcagcatg gaaatcaatt
     9301 tttgcgctta ttgtgccagg actaggcgga acatttgggc tctttattat gcggcaattc
     9361 tttatgaata taccatatga cttagtcgaa agtgcgcaaa ttgaaggctt aggccatttt
     9421 ggtgtgtatc gtaaaattat tttaccctta gggaaatccg cggtgatggt tgcgggacta
     9481 tttgctttta tgggaagttg gaatgactac ttatggccga ctttaaccgc tgcagtgcac
     9541 tttgatcaaa attggtatac attacaaact gggttagcga caatgcaaag tcaaagtaat
     9601 tatgaccccg gtcaagtaat ggcagcagcg attatttcga ttattccagt attaatcgtt
     9661 tatatgtttg ttcaagataa gattattgaa ggtgtcagtc acaccggtat taaatagaag
     9721 ggagcattaa ctatatgtat cgcaaagaat atccgcgtcc cgctcttgtg cgcaaagagt
     9781 ggctaaatct aaacggaata tgggattttg cttttgatga tgataatgtt gggttaaacg
     9841 aaaattgggt tgaaaaggga aagtttccct taaaaataaa tgtaccgttt gcgttccaaa
     9901 cgccattaag tgggattaat gaccctagtt ttcatgatta tgtgtggtat cggcgtttct
     9961 ttagtgttaa aaaagaagaa ggtaaacgct ttatcttaca ttttggtgcg gttgattatg
    10021 aatgtaaaat atatttaaat aaaaaacata ttggtgatca tattggtggc caagttccat
    10081 ttagttttga tgtcactgat tttttaaatt ttaatgaaga agaactagta gtgtgggtta
    10141 gtgacccaag tacagaatta agtatgccgc gtggtaaaca atactggaaa gaaaaatcag
    10201 aagtaatctg gtataaccgc acgaccggaa tttggcaaac agtgtggtta gaaatcgttc
    10261 cagaggcata tgttaaaagt atgcgcatta ccccactttt tgatgaaggt aaggttcatt
    10321 tttccttaag ttttagtcaa gttggtagag aacttattct tgaagatgaa aatggcaaaa
    10381 ttaaatatgc taaagtacct aataaattaa cacatgaatt tactgttcaa acttttttag
    10441 acgagcaaga ttttataaca aaagcgtggt caccagaaaa cccgcattta ttcccgttta
    10501 aattacggta tggtgatgat ttgatttcaa gttacttcgg tatgcgcaag gttcacgctg
    10561 ataaggggat tacatattta aataataagc cctattatat gaaacttgtg ctcgaccaag
    10621 gttattggcc acaaagctta gtgacgccac cgagtaaaga agcgctagaa tatgatataa
    10681 aaatggcgaa agcaatgggc tttaacgggg cacgtaagca tcaaaaatgt gaagatcctt
    10741 attttgctta ttacgctgat aaattaggtt tcttagtgtg gggtgaaatg gctagttcga
    10801 ttcgctttag tgaaaaatca gcgtggcgtg ataaacatga atggccacaa attattgctc
    10861 gcgactataa tcatccgtca atcgtcgcgt gggtcccact taatgaaagt tggggtgttg
    10921 atgatattgg tcacgataaa cggcaacaac aacatgcact tgaattgtat tatttaatta
    10981 aaagtttaga taatacgcgc ttcgttgttg ggaatgacgg atgggaacaa ataattaccg
    11041 acatttgtgc gattcataat tataatcatg gtcgtcatga tgataaagac gccattgctg
    11101 cttttaataa atcaattagc acaaaagaag aattactgaa gtcaagacca gcgaatcgcg
    11161 atatatatgc agagggttat agttatcgcg gtgaaccgat tcttttaact gaatttgggg
    11221 gaattagtta tcaaatgaat gaagataagg gttggggcta tactgcggtt ggtgacgcag
    11281 agcatttact aagtgaatat aaacgcatta ttttagcaat taaagaaagc actgctcttg
    11341 tcggatattg ctatacgcaa ttaactgatg ttgaacaaga aattaatgga ctattaacat
    11401 atgaccggaa gccaaaactt ccagttgaaa aagtaaagga ggtgaatgac ttaattcaaa
    11461 aatataagta gttagcacat cttttcgcca ctgtaggaac gagcactacc cataattaag
    11521 aggcgaaaaa aagattacta ataaagtttg atgaaaggag aaaatatgaa aaaaagaaca
    11581 tcaaaattaa tcatgttatt acttggtctc tctttacttg cgggttgttt taaaacacct
    11641 tcaagtgaaa aaccgccagt aagttcggaa cagccacctg taacaa
//
LOCUS       DAOSTA010000032        10612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_6095_length_10612_cov_7.098986, whole genome shotgun sequence.
ACCESSION   DAOSTA010000032 DAOSTA010000000
VERSION     DAOSTA010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 10612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10612
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6095_length_10612_cov_7.098986"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..657)
                     /gene="yihA"
                     /locus_tag="PLU08_03785"
     CDS             complement(64..657)
                     /gene="yihA"
                     /locus_tag="PLU08_03785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOQ70845.1"
                     /translation="MINFQNTLFVGSFTNKNEGAIEALPEIIFIGRSNVGKSSLINAI
                     INRKNLAYTSKKPGFTKLLNYYNVDDTFYLVDAPGYGFAKREKDLSASFATMMNNYFN
                     KNTNLSMIYLLIDSRRTLTADDEAFLDFVSHHKFPLTLIFTKSDKLNQSMRAKALNFY
                     KKTFKGEVKAIFTSSHNKENISKVHEHISDTLKNLKA"
     gene            complement(665..3013)
                     /gene="lon"
                     /locus_tag="PLU08_03790"
     CDS             complement(665..3013)
                     /gene="lon"
                     /locus_tag="PLU08_03790"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOQ70846.1"
                     /translation="MANKNTAKRYELPLIITRNQVYFPFNQSDDLDAGRPATVEAIKY
                     ANNSDDKLLIVATQKVFTVNNPSMNDIYEVGVLARLTKVRERPHHITVQLEPLARVFI
                     NELDESGPFIIADATVTFHEPLDPDANPERLKNALFDFFNDKLELTRRLHIDLDALKA
                     ADMNLEELIYFLANKTLNSTEQRMAVLAENSLERRIDILLKFLEDDVANDAVGAITQI
                     VRNRPQSGEGLDEVERDGLPLEDDEDEDEDEEFDTGEEILAQLKKRYFPKNFVTRIKK
                     EAKKMGRVEPSERSRIIEYLDWLMKLPYDQETIDNLDLENVRKVLDKDHFGLEEPKKR
                     IVEFMAVKRLAKENRATVLCFYGPPGTGKTSLATSIATAMGRKLVKASLGGVDDESKL
                     RGFLRTYVGAQPGIIISSMRKAGTINPIFVLDEVDKLGKSNRGDPSSALLEILDPEQN
                     KGFIDHYIDEPYDLSKVVFIATANYYWDIPRPLRDRMEMIELKGYTEEEKVQIAEKHL
                     IPNGIESHGLKGYDIKFTDEALRFIIGGYTWEAGVRMLNQKISAIFRKLSVEVLSDVE
                     SNFTITPEEVQKHLGKPRNFFTKKEPEPIVGVVTGISVAGNIGDIMKIEVTTFKGKGV
                     VNVTGNLEKMLKESGEIAASHVRSYAEKYGINPDVFLDTDINVHFPQASTKDGNSAGV
                     AMTIGIISVLTDRKINPNVAMTGEVTLTGRVLAIGGLYEKILGCIRAGIETVLFPEEN
                     VVDLDEIPQEVKDKVKLVPIKNIDEAVAIALLPKKVKKKATA"
     gene            complement(3057..4331)
                     /gene="tig"
                     /locus_tag="PLU08_03795"
     CDS             complement(3057..4331)
                     /gene="tig"
                     /locus_tag="PLU08_03795"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOQ70847.1"
                     /translation="MKLKVTKLETSKYELEVEVSEAKWSAAREKAFKKLAKDVTVQGF
                     RKGKAPEHLVRAKIDPVKELDEAVHSLLDEAFQFAIKESGHYPVIQPTYDVVKVSDTE
                     LVLKFIIVAAPVVTLGQYEGLKIGRGEANVEDSAVEVRLNSILEQNAELILKEDAAAL
                     GDTVVLDFEGFVDGEAFEGGKATNHELELGSGQFIPGFEDALVGVKAGESRDVNVTFP
                     ENYHEQLAGKKAVFKTTVHEVKEKRLPALTDDFAASLNMPNVTDVASLRVHVLFELTK
                     QAEAAERNEYVEKILAKLRETSTFELANDIIDREVEHMDERLKNQVTQQGMEWKQYLE
                     LTKQTEEEIKERFVSEATVNLQNFLVLNEIAKAKEIEVTDAEVDFEIAMMAQQYNMEE
                     KRIRELLGDNINNLRNDVRQKKIINFLLENNE"
     gene            complement(4475..5392)
                     /locus_tag="PLU08_03800"
     CDS             complement(4475..5392)
                     /locus_tag="PLU08_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70848.1"
                     /translation="MMDKLIDYFERGEIDKVIALSKGSKDPEIQFFYLAALRYLGEYQ
                     IALSFISENQMQLYTNNAPQLIDWHIDILLELDDLDQALNTLKIYETFPYFSLETNEL
                     IAKLGDKVQQKRKEKNRQHKFDLFELERRLLCRNVELTFSAVSYMVSNFHEAYIALFK
                     RALLDAPINNVKSIIIFALKELKHNETVQVNKFGKLIKVNPATAPDPFKTKGGAKLIK
                     KMQEVAALDDYNQFSEVADSLITGHAMYIYPLTYEVKDVDGLGDAYLYYIYRALGRVN
                     NVNEYIEEHGLNAETLFAIFTKYHFTYFD"
     gene            complement(5441..6805)
                     /locus_tag="PLU08_03805"
     CDS             complement(5441..6805)
                     /locus_tag="PLU08_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004462370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOQ70849.1"
                     /translation="MVPFLLSSPFKDTSVPTVLMALSIMLLVGFLLTRLTKPLKLPNV
                     TAYIFAGIILGPLAQLILGGKPLFPDELRKGMEFITDLALAFIAFGVVRYFKFDILKK
                     SGPKILIITLFESLMAVAFSFGAMMLMNLIPGVKMDWELAILLAAIASATAPASTMMT
                     IRQTGAKGDFVNTILQVVSLDNAIALILFSVAMAIITGKESGGINFMQLAKPILLNLA
                     LIVAGYLMGLLLYWVTPPKRTKDNRLIIAVAFMLGFSGVASLLDISPLLGVMAMGMAY
                     TNIAPDESLFRQIDAFSPPILTIFFVLSGMRLQLHMLASIGVVGVVYFIVRIIGKYTG
                     ATLGSIVARSTENNKKYLGLALIPQAGVSLGLAALGSRILMGYGHNDYADMLTTIIVA
                     AGVLYEIFGPPAAKYSLSITKSYTTEKTVEMIGPKPSEIPVSAEPKEVPVEESIAEIK
                     AETK"
     gene            6927..7805
                     /locus_tag="PLU08_03810"
     CDS             6927..7805
                     /locus_tag="PLU08_03810"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOQ70850.1"
                     /translation="MIDDKLKTLIILAKVKNYTQTAELCNLSQPAVTQHIKALESRYG
                     IEIFRRNGRNLTLTYEGKSLVDSAKKLIALERNIQKEIKKQKGQPKKLDIGITLTAGG
                     YFIPEIMEVFKDKYPNLRFNFHTDIAENILERMRLNELDFAIIDGTSQTDEFNIELLF
                     RDELIIIGPKDHRLADKKNVSIEELKKEKFILRHEKANTRLAFENYLSNHFDNINNFD
                     VILEIDNTAMIKQLIIAGHGLSVMSKAICEINMQVGTLKQIDLRDFHLERGIYLVYPK
                     ELENSEIIDSILALKE"
     gene            complement(7852..8013)
                     /locus_tag="PLU08_03815"
     CDS             complement(7852..8013)
                     /locus_tag="PLU08_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70851.1"
                     /translation="MEKLVFETIESLLEIKKLRIEERGPVLSRLTDDEIVLLIDNLVE
                     DLQSEYLVQ"
     gene            complement(8168..8968)
                     /locus_tag="PLU08_03820"
     CDS             complement(8168..8968)
                     /locus_tag="PLU08_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70852.1"
                     /translation="MIKRRPEFDPFRDKKVFRCKFCNFPITNPGLYPGKKIMINRYCP
                     ACHSNNTFALLNKQDQTFLRMGFFSNPLYPISPHHLKGVPLKFSLIVFPLSVFFNFVA
                     NIVYMPFSLIYNNIQIKKALKEFYASYKDQDTNLILIDMLEKKAGPKNQYASYKTGIA
                     YLEGIHVPYDLEKAESYFKIAKEEHEGGLVKLAETLIRSENPNYDKAFTILNLVKDNP
                     LASYYLAYLYFYGYGVTPSLRIATIYAGTAHTHNIKDYYDIYPKILQI"
     gene            complement(9116..10594)
                     /locus_tag="PLU08_03825"
     CDS             complement(9116..10594)
                     /locus_tag="PLU08_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70853.1"
                     /translation="MRFSKKVTRKGLIVTFTAAIIAAGSTFLSITKQPVATKAVPTSV
                     SFSTTAQGWTNNQVIDEFDIDTNWNVRLSQKGGTTPPTYYNNGTALRMYGSGVGDGTE
                     LLVQPIATNSDQVLRKFVITSSGTSYTPAIKVWTGETEDSLVEVVPAAAWSGTSYTWT
                     GTTNIRFIKLKMVANATTQIRLRNIDLEYDTGTLTYDPITNLDASSMNNYALKVGERF
                     RVAPTISPATAYKGVTLGVSDATAVSVSGMDIIGLKPATGIIITVTSVAQTSGGTNLV
                     DTFTVNVDYETVTVAEALLLPSGTGQIYKVIEAKIEPNYTVTDREILLVDKLDETKTM
                     LIYQYGINPPAVKRYIAEGLISFTATIGTYGGKNQFTSPTITAYTDEVEEFATSILTG
                     DTAGQCNTRFADYKTQVLAFSAAEKDKIQNGTDTNIVNARARYLAWAAALGERPWEAG
                     LVAVNQTVNNESNNINIIFIIIAIFGVALYGLVSFRKRKYNN"
BASE COUNT         3267 a         2048 c         1765 g         3532 t
ORIGIN      
        1 atctattttt aaaactttat tttctatttt gtcactttat ctttttcatg taacacaagt
       61 tacttatgct ttaaggtttt tgagtgtatc actaatatgc tcatgcactt tacttatatt
      121 ttctttattg tgactactag taaaaatcgc tttaacttca cctttaaatg tctttttata
      181 aaagtttaag gctttcgcgc gcatactttg atttaattta tcagactttg taaaaataag
      241 ggtcagcgga aatttatgat gactaacaaa gtctaaaaaa gcttcgtcat ccgcggttag
      301 tgttcgtcta ctatcaatta gtaaataaat catggataaa ttagtgtttt tattaaagta
      361 attattcatc atagttgcaa aactagcgct taaatctttt tcacgctttg caaacccgta
      421 gccaggagcg tcgactaaat aaaatgtatc atcaacatta taatagttta ataatttcgt
      481 aaatccaggt tttttggaag tatacgctaa gtttttacga ttaataatcg cgttaattaa
      541 actactttta ccaacattac tacgcccaat gaaaataatc tcgggcaaag cttcgattgc
      601 cccttcgttt ttgtttgtaa aactaccaac gaacaatgta ttttgaaaat taatcatagt
      661 acttttaggc agtcgctttt ttctttactt ttttcggtaa aagcgcaatg gctactgctt
      721 catcaatatt tttaatcggc acaagtttaa ctttatcttt cacttcttgc gggatttcat
      781 caaggtcaac aacattttct tcagggaata atactgtttc aatccccgct ctaatacagc
      841 ctaaaatctt ttcataaagc ccgccgatgg caagtactcg gccagttaaa gttacttcac
      901 cagtcatcgc cacatttgga ttaatcttac gatcagttaa tacactaata atgccaatcg
      961 tcattgcgac acccgcacta ttgccatctt tcgtacttgc ttgtgggaag tgtacattta
     1021 tatcagtgtc aaggaagaca tcaggattaa tcccatactt ttcagcataa cttctaacat
     1081 ggcttgcagc aatttcccct gattctttaa gcattttttc aaggttccct gttacattaa
     1141 caacaccttt acctttaaat gttgttactt caatcttcat gatgtcacca atattaccag
     1201 cgacactgat gccagtgaca acaccgacga ttggttctgg ttcttttttg gtaaaaaagt
     1261 tacgcggttt acctaaatgc ttttgcactt cttcaggggt aatcgtaaag ttactttcca
     1321 cgtcacttag tacttcaaca cttagcttac ggaaaatggc gctgattttt tgattaagca
     1381 tccgtacacc agcttcccaa gtatagccac cgataataaa gcgtaaggct tcatcagtaa
     1441 acttaatgtc atagccctta agcccgtgac tttcaatccc gttaggtatt aagtgctttt
     1501 cagcaatttg tactttttct tcttcggtat acccttttaa ttcaatcatt tccatccggt
     1561 cacgaagtgg acgcgggata tcccagtaat agttagcggt ggcaataaag acgactttac
     1621 ttaagtcata aggttcatca atatagtgat cgataaaacc tttattttgt tcagggtcaa
     1681 gaatttctaa aagtgcactg cttgggtcac cacgattgct cttccctaat ttatcgactt
     1741 cgtcaaggac gaaaattgga ttaatcgttc ctgctttacg cattgaggaa ataataatgc
     1801 cgggttgcgc accaacgtaa gttcgtaaaa agccacgtaa cttactttcg tcatcaaccc
     1861 cacctaaact agctttgaca agtttacggc ccatcgcggt tgcaatactt gtcgctagac
     1921 tagttttacc agtgccaggt ggtccataaa aacataaaac cgttgctcta ttttctttag
     1981 caaggcgttt tacggccata aattcgacaa tgcgtttttt tggttcttct aaaccaaagt
     2041 gatctttatc taagacctta cggacatttt caaggtcaag attatcaatt gtttcttggt
     2101 cataaggtaa cttcattaac caatctaaat attcaattat ccgtgagcgt tcacttggtt
     2161 caacgcggcc catctttttc gcttcttttt taatgcgcgt tacgaagttt ttggggaagt
     2221 agcgcttttt aagttgcgct agaatttctt caccagtatc aaattcttcg tcttcgtctt
     2281 catcttcatc gtcttctagc ggtaagccgt cgcgttcaac ttcgtcaagt ccttcaccgc
     2341 tttgtgggcg gttacgcaca atttgtgtaa tcgctccaac agcatcatta gcgacatcgt
     2401 cttcaagaaa cttgagtaaa atatcaatgc gtcgctctag actattttcg gcaaggacag
     2461 ccattctttg ttcggtgcta ttgagcgttt tattcgctaa gaagtaaata agttcttcta
     2521 aattcatatc ggcagccttt aaggcatcta aatcgatatg taaccgtctt gttaactcta
     2581 atttgtcatt aaagaaatca aataaggcat tttttaagcg ttcaggatta gcgtcagggt
     2641 caagtggctc atgaaaagta acagtagcgt cagcaataat aaagggacca ctttcatcaa
     2701 gttcattaat gaaaacacgg gcaagtggtt caagttgtac ggtaatgtga tgcggtcttt
     2761 cacgcacttt tgttagtcgt gcgagtacgc cgacttcata aatatcattc atcgatgggt
     2821 tattaaccgt aaaaaccttt tgcgttgcta caattagtaa cttatcatca ctattattag
     2881 cgtatttaat cgcttcaaca gtcgccggtc gtcccgcatc taagtcatca ctttgattaa
     2941 acggaaaata cacttgattg cgtgtaataa taagcggtaa ctcatagcgt tttgctgtat
     3001 ttttgttagc catgtaacct ctccttttct ttatgtaaaa cttaactaat taataattat
     3061 tcattatttt ctaataagaa attaataatt ttcttttggc ggacatcatt acgtaaattg
     3121 ttgatgttat cgccaagtaa ttcacggatg cgtttttctt ccatgttata ttgttgcgcc
     3181 atcatcgcaa tttcaaaatc gacttccgca tcagtaactt caatttcttt agctttggca
     3241 atttcattaa gcactaagaa gttttgtaag ttaacggttg cttcactcac aaagcgttct
     3301 ttaatttctt cttcagtttg ttttgttaat tctaaatatt gtttccattc cattccttgt
     3361 tgcgtgactt gatttttaag tctttcatcc atatgttcga cttcacggtc aataatgtca
     3421 ttcgcaagtt caaaggttga tgtttcgcgt aatttcgcta agattttttc aacatattca
     3481 ttgcgttcag cggcttcggc ttgtttagta agttcaaata agacatggac gcgtaaacta
     3541 gcaacatccg taacatttgg catattaagt gaggctgcaa agtcatcagt taacgctggt
     3601 aaacgttttt ctttaacttc atggactgtt gttttaaaga cagctttctt accagcgagt
     3661 tgttcatgat aattttcggg gaaagtaacg ttaacatcac ggctttcacc tgcttttaca
     3721 ccgacaagtg catcttcaaa acctgggata aattgaccac tgccaagttc taattcgtga
     3781 ttagtggctt taccaccttc aaaagcttcg ccatcgacaa acccttcaaa gtcaagcaca
     3841 acagtgtcac ctaaagcagc agcgtcttct tttaagataa gttcggcatt ttgctctaag
     3901 attgagttaa ggcgtacttc aacggcactg tcttcaacat tagcctcacc acgaccgatt
     3961 ttaagccctt catattggcc aagtgtaaca actggtgcag caacaataat aaatttaagg
     4021 acaagttcag tatcacttac tttaacgacg tcataagtag gttgaattac tggataatgt
     4081 ccgctttctt taatcgcaaa ttggaaagct tcatcgagta aactgtgcac tgcttcatct
     4141 aactctttaa cgggatcaat tttagcgcgc actaagtgtt ctggtgcttt acctttacgg
     4201 aagccttgga cagtgacgtc ttttgctaat ttcttaaagg ctttctcacg tgccgcactc
     4261 catttagctt cactaacttc gacttctaat tcgtatttac ttgtttcaag tttagttact
     4321 tttaatttca tatataaaaa ttcctccatt tataacctta gtaagtatac taaaatatcg
     4381 ctcattttag taataaagtg gcttatttat gtaatatttc cgtgattaag gacaatttaa
     4441 tcattttatt tcgttaaatc ataaaaagac atgattaatc aaagtaggta aagtgatact
     4501 ttgtaaatat cgcaaatagt gtttcagcgt ttagaccgtg ttcttcaata tattcattaa
     4561 cattattaac acgccctaac gcacgataaa tgtaataaag gtaggcgtcc cctagtccgt
     4621 caacatcttt tacttcgtaa gtaagcgggt aaatatacat cgcatgacct gtaattaaag
     4681 aatcagcgac ttcactaaat tgattatagt catcaagcgc cgctacttct tgcatttttt
     4741 taattaattt agccccgcct ttcgttttaa aaggatcagg cgctgttgcg gggtttactt
     4801 taattaattt cccaaactta ttcacttgca cagtttcatt atgttttaat tcttttaagg
     4861 caaaaattat tattgattta acattgttaa ttggtgcatc aagcagcgct cttttaaata
     4921 gggcaatata agcttcatga aagttactta ccatataact aaccgcacta aaagtaagtt
     4981 caacattacg gcacaatagt cttcgttcta attcaaaaag gtcaaattta tgttgcctgt
     5041 tcttttcttt acgtttttgt tgaactttat cgcctaattt agcgataagt tcatttgttt
     5101 ctagactgaa gtatgggaaa gtttcataga ttttaagtgt gtttaaagct tgatctaaat
     5161 catctaattc aagcaatata tcaatatgcc aatcaataag ttgcggagca ttatttgtgt
     5221 ataattgcat ttgattttca ctgataaatg atagagcaat ttggtactcc cctaaataac
     5281 ggagcgctgc taaataaaag aactggattt caggatcttt actcccttta cttagcgcga
     5341 taactttatc aatttcaccg cgttcaaaat agtcaattaa tttatccatc ataataaggt
     5401 tatattagca taatatatgc tattaagtta ttaaatataa ttattttgtt tccgctttaa
     5461 tttcggcgat actttcttcg actggtactt ctttaggttc agccgaaaca gggatttcgc
     5521 ttggttttgg tccaatcatt tcgaccgtct tttcggtcgt ataagacttc gtaattgaga
     5581 gtgagtactt cgctgctggt ggtccaaaga tttcataaag cacaccggcg gcaacgataa
     5641 tagtcgtcaa catatcagcg taatcattat ggccatatcc cattaaaatc cgtgaaccaa
     5701 gcgcggcgag ccctagacta acacccgctt gcgggattaa agcaagacct aagtacttct
     5761 tattattctc ggtgctacgc gccacgatac taccaagcgt cgcacctgta tacttaccaa
     5821 taatacggac gataaagtaa acaacaccta caacaccgat actagcaagc atatgtaatt
     5881 gtaaccgcat cccacttaat acgaagaaaa tcgtcagaat cggtggtgaa aaggcatcaa
     5941 tctgccggaa aagtgattcg tctggtgcaa tattagtgta agccatcccc attgccataa
     6001 cgcctaaaag tgggctgatg tctaagagtg aagcgacccc gctaaagcca agcataaagg
     6061 caacagcgat aataagccgg ttgtctttcg ttctttttgg cggtgttacc caatataaaa
     6121 gtaaacccat taagtaccca gcgacaatta aagcaaggtt taaaagaata ggtttagcaa
     6181 gttgcataaa gttaatgccc ccactttctt taccagtaat aattgccatc gccacactaa
     6241 aaagaattaa agcaatggcg ttatcaagcg acactacttg taagattgta ttaacaaagt
     6301 cccctttagc gcctgtttgt ctaattgtca tcatcgtgga agcaggtgct gtagcgctag
     6361 caatcgctgc gagtaaaatc gctaattccc agtccatttt aactccggga attaagttca
     6421 tcagcatcat cgctccaaag ctaaaagcaa cagccattaa gctctcaaat aatgtaatta
     6481 tgagaatttt tggtccgctt ttctttaata tgtcgaattt aaagtaacgc acaacaccaa
     6541 aagcaataaa cgctaaagca aggtcagtaa taaactccat tcctttacgg agttcatcgg
     6601 ggaataaggg tttaccacct aaaataagtt gcgcaagtgg tccaagaata ataccagcaa
     6661 aaatataagc cgtcacgttt ggtaacttta ggggttttgt gagccgcgtt aaaagaaagc
     6721 caacaagtaa cataatgctt aaagccatca gtactgttgg tacacttgtg tctttaaacg
     6781 gacttgaaag taaaaacggg accataactt gcctccttat atagtgttgt attatgatta
     6841 tacacactta ttactaatat aacaacttat aatttgttat aattatataa gaataactta
     6901 taattagaaa cagaggtaaa tttacgatga ttgatgataa attaaaaaca cttataatct
     6961 tagcaaaagt aaaaaactat acacagaccg ccgagctctg taatctttca caacccgcag
     7021 taacgcagca cattaaagca cttgaaagtc gttatggcat cgagatattt cgccgtaatg
     7081 gccgtaatct tactttaact tatgaaggga aaagccttgt cgatagtgct aaaaagctta
     7141 ttgcgcttga acgtaatatt caaaaagaaa ttaaaaagca aaaaggtcaa cctaaaaaac
     7201 ttgatattgg catcacttta acagcagggg gctacttcat tcccgaaatc atggaggtat
     7261 ttaaagataa atatccaaat ttacgcttta attttcatac ggatatcgcc gaaaatattt
     7321 tagagagaat gcgcttaaat gaattagatt ttgcgataat cgacggcaca tcacagactg
     7381 atgaatttaa tattgaactg ctcttccgtg atgagttaat tattattgga cctaaagatc
     7441 atcggctcgc tgataagaaa aatgtaagta ttgaagaatt aaaaaaagaa aagtttattc
     7501 ttcgtcacga aaaagctaat acacgcctag cgtttgaaaa ttatttaagt aaccacttcg
     7561 ataatattaa taactttgat gttattttag aaatcgataa tacggcaatg attaagcaac
     7621 ttattattgc cggtcacggc ttaagtgtaa tgagtaaagc aatttgtgaa ataaatatgc
     7681 aagttggtac acttaaacaa atcgatttac gcgacttcca cttagaacgg ggaatttacc
     7741 ttgtttatcc taaagagtta gaaaatagcg agattattga tagcattcta gcgcttaaag
     7801 aataaaaaaa cacggccata taccgtgctt cactaaaaaa atgaatactt attattgcac
     7861 taagtattca ctttgtaagt cttccactaa gttgtcgatt aataagacga tttcatcatc
     7921 cgttaatcta cttaatactg gaccacgttc ttcaatccgt aactttttaa tttctaataa
     7981 actttcaatt gtttcaaaca ctaatttttc catactcttt tactccttct ccccactaga
     8041 taagcttttt tgctacaaac gcattatagc ataagactac ttactcacca aagtaatgag
     8101 gagtgttaaa tttgtaaaaa agtctttaca attagtaaag caaacataga atgtatgtta
     8161 ttttatttta gatttgtaag atttttggat aaatgtcgta ataatcttta atattatgcg
     8221 tgtgcgcagt accagcgtaa attgtcgcaa tacggaggct aggcgttaca ccgtagccat
     8281 aaaaataaag ataagcaaga taataactcg ctagcggatt atcttttact aaattaagaa
     8341 tcgtaaaagc tttgtcgtaa tttgggtttt cactgcgaat cagtgtttct gcaagtttaa
     8401 ctaagccgcc ttcatgttct tcttttgcta ttttaaagta agattccgcc ttttctaagt
     8461 cataaggaac atgaatgcct tctaagtacg ctatacctgt cttataacta gcgtactgat
     8521 tctttggacc agctttcttc tctaacatat caattaagat taaattagtg tcttggtctt
     8581 tataagaagc gtaaaactct ttcagtgcct tcttaatttg aatattgtta taaattaagc
     8641 taaacggcat atacacaata ttcgcgacaa agttaaagaa cacacttaag gggaacacga
     8701 ttaaactaaa cttcagtggc acgcctttta agtgatgtgg gctaattgga taaagcgggt
     8761 tactaaagaa acccatccgt aaaaatgttt gatcttgttt atttaaaagc gcaaaagtgt
     8821 tattgctatg acacgctgga caatagcgat taatcatgat cttcttccca ggataaagtc
     8881 cagggttagt aattgggaag ttacaaaact tacaacggaa tactttttta tcgcgaaacg
     8941 gatcaaattc aggccttctt ttaatcataa gttactctta cctccactta gtatattata
     9001 gtaaatttaa ataattatta tttaaaaaag gataatatta tcataacttc ctttcataag
     9061 aaaaagtcag gaatttgaca tcctgactct ttcgttattg gtaagctaca ctttactaat
     9121 tattgtattt gcgtttacgg aaagaaacta aaccatataa agctacacca aagatagcaa
     9181 ttataataaa tattatgttt atattgttac tttcattatt aactgtttgg ttaacagcaa
     9241 caagtcctgc ttcccatggt ctttcgccta acgctgctgc ccacgcaaga taacgagcac
     9301 gagcattaac aatattagtg tctgtaccat tttgtatttt atctttttcc gctgcactaa
     9361 atgctaacac ttgagtttta taatctgcaa aacgagtatt acattgacca gcagtatcac
     9421 cagttaaaat acttgtggca aattcttcaa cttcatcagt gtaagcagta atagttgggc
     9481 tagtaaattg atttttaccc ccataagttc caatggttgc agtaaaggaa atcaaacctt
     9541 ctgcaatgta acgtttaact gcaggtggat taatcccata ttgataaatt aacattgtct
     9601 tagtttcatc aagcttatca accaataaaa tttctcggtc agtaacagta taatttggtt
     9661 caatctttgc ttctatcact ttataaattt gaccggttcc agatggtaaa agtaatgctt
     9721 cggcgacagt tacagtttca tagtctacat taacagtaaa tgtatcaact aaattagtac
     9781 cacctgaagt ttgggctact gaagtaacgg taataatgat gccggtcgcg ggtttaagac
     9841 caatgatatc cattccgctt acactaacgg cagttgcatc actaactcca agagtaactc
     9901 ctttataagc cgttgcaggt gaaatagtag gagcaactct aaatctttca ccgaccttaa
     9961 gtgcataatt attcatcgaa cttgcatcaa ggttagtaat tggatcataa gttaatgtac
    10021 ctgtatcata ttctaaatcg atatttctta agcggatttg ggttgttgcg tttgcaacca
    10081 tttttaattt gatgaaacgt atattagttg ttcctgtcca agtgtaactt gtcccgctcc
    10141 aagctgctgc tggaactact tcaactaaac tatcttcagt ttctccagtc caaactttaa
    10201 ttgcgggtgt atagctagta cctgaagaag taataacaaa tttacgtaaa acttgatcac
    10261 tattagtagc tattggttgt actagtaatt cagtaccatc accaacgcca ctaccataca
    10321 ttctaagtgc tgtaccgtta ttgtaataag ttggtggtgt agttccacct ttttgtgaca
    10381 aacgaacatt ccaatttgta tcgatatcaa attcatcgat tacttgatta tttgtccaac
    10441 cttgagcggt agttgaaaaa ctaacacttg ttggtactgc ttttgtagca acaggttgtt
    10501 ttgttataga aaggaatgta ctacctgcag caattatagc tgctgtgaat gtaacaatca
    10561 atcctttcct tgttactttt tttgaaaacc tcataaatat ctcctttcta at
//
LOCUS       DAOSTA010000033         9650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_6823_length_9650_cov_10.411881, whole genome shotgun sequence.
ACCESSION   DAOSTA010000033 DAOSTA010000000
VERSION     DAOSTA010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9650
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6823_length_9650_cov_10.411881"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..428
                     /locus_tag="PLU08_03830"
     CDS             63..428
                     /locus_tag="PLU08_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOQ70854.1"
                     /translation="MGLKPSGEMYLKTIYILTKTVHPVRSIDIAEYMNFSKPSVSRAV
                     GNLRKEKYISVNEDGHIFLTPKGLDAAQNIYDRHKVLTKALRILGVSEETALNDACEI
                     EHIISDESFEALKQYLKNK"
     gene            complement(711..2984)
                     /locus_tag="PLU08_03835"
     CDS             complement(711..2984)
                     /locus_tag="PLU08_03835"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOQ70855.1"
                     /translation="MKKNIRVALLGNPNSGKTTLFNSLTGSTAYVGNWPGVTVEKKSG
                     TYYFKGKGKDREGIDIIDLPGIYSLSPYTPEEVISRNYILKENPDVVINVIDATNLER
                     NLYMTTQLLEIDVPVVLAVNMTDALAESGKTIDYAKLKEKLKIPVVPISALEEKGLDN
                     LMKVTKVEANKKREAYSVITDKKIEKVTEIYKKYKIANPLFHAIKALEKDEIEESENK
                     EAYADVQSLMAQDKEDYEAVIADLRYQFLTPLCAEISTKPHKSEKEKWTKSDRIDQVL
                     THKIFGMLIMIVALFFVFHFTFSGDLLFLNAFGVNFGEGYEGFLSIMVGGEEYRPFAG
                     LIYTDGGINSIGEFFHVLAGDNETGILGLISLGIKALLLKWNAPEWVVGFFYDGLLNG
                     VTAVLGFVPQIMILYTFFSIMEDSGYMARIAFMLDRIFRKFGVSGRAFIPMIMGFGCA
                     IPAMINTRTLATEKERIKTIRVIPFFTCGAKAEFIAIIAAAIAAAVGVDAGLFTFSMY
                     MLGILVAIVSVIVMNKTTQREKVPPFIMELPTYRTPQLKALTRHVYNKGKHFVIKAFT
                     IILASTIVIWLLANFGWDWQMVDENGTGSILQSLGSFIQPLFTPLGFGVQAGEHGWTL
                     TVASIQGIVAKENVTATAETLASIIDVDGFTGIVEAIGLSNAAVVAFSVFNLLTIPCF
                     AAIATAKGELSNRKVYRWTIAYWFLLSYGLGALTYVSFAYVWTLAITIPVIALLIALL
                     IIYDRHKKKQELLLENE"
     gene            complement(2986..3273)
                     /locus_tag="PLU08_03840"
     CDS             complement(2986..3273)
                     /locus_tag="PLU08_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOQ70856.1"
                     /translation="MNEEKIIIKVGDLAKLSIVPIGTSGVIHLVSGQGRLRRRLLDMG
                     LTPGAEVFLRKQAPLGDPLELTLRGYELSIRKSEADLIVIRVTDLDAKGDK"
     gene            complement(3288..3500)
                     /locus_tag="PLU08_03845"
     CDS             complement(3288..3500)
                     /locus_tag="PLU08_03845"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOQ70857.1"
                     /translation="MILPIANTERDYVILEVAGTDKLRQYLASLGIIKGKSLRIMSQG
                     KTGTIILVNETRLALNYDIAKLITIA"
     gene            3799..4557
                     /locus_tag="PLU08_03850"
     CDS             3799..4557
                     /locus_tag="PLU08_03850"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOQ70858.1"
                     /translation="MDYNALLPIIFYLCACFYSFFGIYTLATNAKSRTNWQFFFLMIS
                     LTIWSFTYAMAYSVDSAETRIMWKSLGVFGWSLFYAFFLRFAIILTKRNEPSKKPFLQ
                     VLFYLPALITIILFGPFGFLMGMQYEFVPGETGLFQAIINNIGQIWFGLYPSAYTIIS
                     LVILIRWRRTIDRESPLRRLLSIFLISIILPFIIGIFLGVFPRFFGLENLPRLTVAFL
                     IPPAVLLFIALRKFGMVFETKTRRDFSPLDPKTT"
     gene            4570..6042
                     /locus_tag="PLU08_03855"
     CDS             4570..6042
                     /locus_tag="PLU08_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOQ70859.1"
                     /translation="MRLFQTAALIFGIGAAASFFSGYFLTNGDFIREIILSVSIWFFA
                     IFFLFVPHITKKILIQNILFFITSVVGMTFFIVKDAHMGASTVWAVYIVFLLYSIVLD
                     SKRDTLFFLIFTVFTQIVLWITFPNVTIVLDNAQYAKRLFIIILSYVAVRYVTSEYDS
                     KFQGYQRFSRGQEILEKISTNFIAVNQDNVRAKIDEMFMMAAEILQYDQAYLFRFDDK
                     GERATILNAYVRDSEDNSLLLQLGRSFKISEFPQVEPLAGKHSPIFCDDIANLGTHTQ
                     DESANFFLSRGINSFYALPMFVEEKLSGFFVVEYKEKNELTVVESRLHYLKIIANVLG
                     DARKKIIYEKQLYDFAYIDKSTNLPNRNMLINNLEELLLNRDDADKLVVFNVELDNLR
                     MINDTFGHATGEQVVIKSASILQTLMKDGCVLSRIAEDKFIIVMPIAETIAQVMDCAN
                     EITDAFVNPILPADSIGSLFVTTIVGVATFPEDGRDATPF"
     gene            6123..6899
                     /locus_tag="PLU08_03860"
     CDS             6123..6899
                     /locus_tag="PLU08_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOQ70860.1"
                     /translation="MEEYTLLTNKLFSSLKNKEFNLQFQPQINCNNGQINGVESLLRW
                     TTDEGQRVGPNVFIPMLEQTGLIHEVGLWVIDQSLKQHKALVKKGFAPLRFSVNLSVA
                     QFRRDDFVDNVLKLLKKHKVEPQYLELEITESLLSKNFADTIEKLTRLNDAGLHIAID
                     DFGKGYSSLHRLQLVPFNRIKIDKSVVDNITIEPKKVVIAKTIINLAKSLNANITTEG
                     VETKEQAAFMKDLGTDEIQGYYYYSRPLPPKDLEDFLKQK"
     gene            complement(6948..7553)
                     /locus_tag="PLU08_03865"
     CDS             complement(6948..7553)
                     /locus_tag="PLU08_03865"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOQ70861.1"
                     /translation="MHKVSIRSKLTPHQVFTIPNILSFFRLLLIPLIVFLYVGKELYG
                     WTLVVLIISLVTDIIDGLVARKLNMVTDFGKFIDPVADKLTQLAVLFCLVTRFPLMWI
                     PISIMLLKEILQFALRFVVFRYTGEVHSAEWHGKVATFLIYVMMSLHVIWYGITDVAS
                     TLTITITSLSMLLSCVLYTISSAVIIINIKRRKKDKEQLNN"
     gene            complement(7603..7687)
                     /locus_tag="PLU08_03870"
     tRNA            complement(7603..7687)
                     /locus_tag="PLU08_03870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7651..7653),aa:Leu,seq:cag)
     gene            complement(7717..9000)
                     /locus_tag="PLU08_03875"
     CDS             complement(7717..9000)
                     /locus_tag="PLU08_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOQ70862.1"
                     /translation="MKRKTYLRNQTIYQIYVRNHTAEGTFVALIKDLPRIKELGVDIL
                     YLLPIHPIGEIARKGELGSPYAIRDYYLINPEHGTLEDFQTFITLAHSYGFKVMIDIV
                     LHHTSPDAELLAEHPEFYYYKNGKLGNKVGDWSDIIDLDYNNRDLWDYMVEMLKYWAS
                     LGVDGYRADVAPLVPLAFWEYARQELDEDYPELIWLSESVEPHFITNLTAAGHVAHHD
                     KEMYSAFDILYDYDVYNDLKAYLEGKAPLKPYLDGVRYQEQNYPKGYLKIRTIENHDT
                     VRIASLCKTDQILRNVTAWSFFQNGVGFIYGGQEVKATHRPTLFDIDKIDLNVKDQDY
                     YDFMILLVEMKKHPVFAEISSFNIIGDGADDVIVSTLESRVGAAHGFFNLGLKEKEVK
                     TELKDGSYTNVLTNTDIVVNDGKIKVSEPIIIIEE"
     gene            complement(9088..9163)
                     /locus_tag="PLU08_03880"
     tRNA            complement(9088..9163)
                     /locus_tag="PLU08_03880"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9128..9130),aa:Ala,seq:ggc)
     gene            complement(9202..9555)
                     /locus_tag="PLU08_03885"
     CDS             complement(9202..9555)
                     /locus_tag="PLU08_03885"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HOQ70863.1"
                     /translation="MKRHEFTLKPIPFMRIKNKQKVIEMRLYDEKRQKLRVGDFIYFT
                     NTETKEILKVEILELTRYLNFTELYAAYTPKELGYLDGEEVAPSDMEEYYKKDDIDRF
                     GVLAIYINVLNSEID"
     gene            complement(9564..9639)
                     /locus_tag="PLU08_03890"
     tRNA            complement(9564..9639)
                     /locus_tag="PLU08_03890"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9604..9606),aa:Arg,seq:cct)
BASE COUNT         3105 a         1807 c         1584 g         3154 t
ORIGIN      
        1 gaaaggagat gtattaccta atcgaagtac cggggcatga ttagataata caagtcggtt
       61 atatgggtct aaaaccatct ggtgagatgt atttaaaaac aatttatatt ttaactaaga
      121 cagtccatcc tgttcggtca attgatattg ctgaatacat gaatttttct aaaccaagtg
      181 tgagtcgcgc tgtcggtaat ttacggaaag aaaagtatat aagcgttaat gaagacggtc
      241 acattttcct tacgcctaaa gggcttgatg ctgcacaaaa tatatatgac agacataaag
      301 tgctaactaa ggcgctgcgt attttaggag taagtgaaga gacggcactt aatgacgctt
      361 gtgaaatcga acatattatt agcgatgaat cctttgaagc acttaaacag tacctaaaaa
      421 ataaataata cttaaacttc ataaaaataa agggctgctt atgaatataa tcttcattaa
      481 cagcccgttt ttaataatca tttcttgttt tatttaagcg acttataatg tttacgataa
      541 tatgacttaa tgtcaatatt tgtcgtggga cacccaccac aactaacccc acatcctgtt
      601 tcgccttttt gtcttttttt aacaaaataa agaatagtgc tagttagtac aacaataata
      661 aatataatcg tgaatatttc tgtccaatgc aaaattttaa tcttcctttg ttattcattt
      721 tctaatagta attcttgttt ctttttatgg cgatcataaa taataagcaa ggcaattaat
      781 agtgctataa ctggtatcgt tatcgccagt gtccacacat aagcgaaact aacataagtt
      841 aatgcgccta acccataact taaaaggaac caataagcaa ttgtccaccg atatactttg
      901 cgattactta attcaccttt agctgtagca atagccgcaa aacaaggaat agttagtaag
      961 ttgaacacgc taaaggccac aacagcagcg ttacttagtc caatcgcttc gacaataccg
     1021 gtaaacccat ctacatcaat aatactcgct agtgtttccg ctgttgcggt aacattttct
     1081 ttggccacta taccttggat tgaagcgacc gttaatgtcc agccgtgttc accagcttgt
     1141 acaccaaaac caagcggtgt aaataagggc tgaataaatg aaccaagact ttgtaaaatt
     1201 gagcctgtac cattttcatc gaccatttgc caatcccaac caaagtttgc taaaagccaa
     1261 atcacgatcg tcgacgctaa aatgatagta aatgctttga tgacaaagtg tttacctttg
     1321 ttataaacat gacgcgtgag tgcttttaat tggggcgtac gatatgttgg taattccata
     1381 ataaagggtg gtactttttc tctttgcgtt gttttattca ttactataac tgaaacaatc
     1441 gctactaaaa taccaagcat atacatggaa aaagtaaaga gcccagcatc aacaccaact
     1501 gctgctgcta tcgctgcggc aataatggca ataaattcag cttttgcgcc acaagtgaaa
     1561 aacggaataa cgcgaattgt tttaattctt tctttttcag tcgctagtgt gcgtgtatta
     1621 atcattgcag gaattgcaca gccaaagccc ataatcattg gaataaaagc acgaccagaa
     1681 acaccaaatt tacggaaaat acgatctaac ataaaggcaa tacgagccat atatccacta
     1741 tcttccataa tcgaaaagaa agtgtaaaga atcataattt gtgggacaaa ccctaaaaca
     1801 gcagtaacac cgtttaatag cccgtcatag aaaaaaccaa cgacccattc aggagcattc
     1861 catttaagta atagtgcttt aattcctaaa gatatcaatc ccagtattcc agtttcatta
     1921 tcacctgcta gtacatggaa aaattcacca atagaattaa tcccaccatc agtgtaaata
     1981 aggccggcga atggtcgata ttcttcgcca cctaccataa tacttaagaa accttcataa
     2041 ccttcaccaa aattaacacc aaaagcattt aaaaatagta ggtctccact gaaagtaaaa
     2101 tgaaatacaa aaaataaggc gacaatcatg attagcattc cgaatatttt atgcgttaac
     2161 acttggtcaa tccggtcaga ttttgtccat ttttcttttt cacttttatg cggttttgta
     2221 cttatttccg cgcataatgg ggttaaaaac tgataacgta agtcagcaat tacagcttca
     2281 tagtcttctt tatcttgtgc cattaatgat tgaacatcag cgtaagcttc tttattttca
     2341 gattcttcaa tctcatcttt ttctagtgcc ttaatcgcgt gaaataacgg attagcaatt
     2401 ttatattttt tataaatttc ggtaactttt tcaatttttt tatcagtaat aacactataa
     2461 gcttcacgtt tcttattcgc ttcaactttt gttactttca ttaaattatc tagccctttt
     2521 tcttctaatg cagaaatcgg cactacaggg atttttaatt tttctttaag ttttgcatag
     2581 tcaattgttt tgccactttc cgctagagca tcagtcatat tcacagcaag aacaacagga
     2641 acatcgattt ctaaaagttg tgttgtcata tataaattac gctctaagtt agttgcgtca
     2701 attacattaa taacaacatc aggattttct tttaatatat aatttcgtga aataacttct
     2761 tcaggggtgt aaggagataa agaataaata cctggtaagt caataatgtc aatgccttct
     2821 ctatctttac ctttgccttt aaaataataa gttccgctct tcttttcaac agtaacacct
     2881 ggccaattac caacatacgc cgttgaacca gtcaaactat taaaaagtgt tgtttttcca
     2941 gaatttggat tacctaacaa ggcaactcta atatttttct tcatcttatt tatcaccttt
     3001 cgcatctaaa tctgtaactc taattacaat taaatcggct tcacttttac gaatagataa
     3061 ttcataaccg cgtaatgtaa gttctaaagg atcaccaagt ggcgcttgtt tacgcaaaaa
     3121 cacttcagcg cctggtgtta aacccatatc tagaagccga cgccgtaacc gtccttggcc
     3181 acttactaag tggattactc cgcttgtacc aattggaaca atacttagtt ttgctaaatc
     3241 tccaacttta ataattattt tttcttcatt catattgcta ccattcctta tgcgatagtt
     3301 attagcttgg caatgtcata attaagggca agacgtgttt catttactaa aataatcgtg
     3361 cctgttttac cttgtgacat aatcctaagt gatttacctt taataattcc tagggacgct
     3421 aaatattgtc ggagtttatc cgtgcccgct acttctaaaa tgacataatc gcgttccgta
     3481 tttgctattg gtaatatcat aaaatcccct agttagtcat tgctaacaag gaaatgttag
     3541 ccgttgctaa ccggtatttc aagcaatatt gtaaatttta aaaaatattc ataaatttac
     3601 atcgaaacat ttattgttat agagtttatt aatgtgatag agtcgtttta taaacagtta
     3661 aaaagagcac caattgtaaa atatattcat aatttctgca tttttttaat attgttgtgc
     3721 tttttttcta actatgtgtt aggatttgct tgtgttaatc aaataattga ttaatagtag
     3781 gaggaacagc aaaaaactat ggattataat gcattgcttc cgataatctt ttatctttgc
     3841 gcctgtttct atagtttttt tggcatatat actttggcca ctaatgcgaa aagtcgcacg
     3901 aattggcagt ttttcttcct aatgatttcg ttaacaattt ggtcatttac atatgcaatg
     3961 gcctactctg ttgatagtgc tgaaacaaga ataatgtgga agtcacttgg tgtttttggg
     4021 tggagccttt tttatgcttt tttcctccgc ttcgccataa ttctaacgaa aagaaatgaa
     4081 ccaagtaaaa agcccttctt acaagtttta ttttatttac ctgcactaat aacaataatc
     4141 ttatttggtc cttttggctt tttaatgggt atgcaatatg aattcgtgcc aggtgaaaca
     4201 ggattatttc aagcaattat taataacatt ggtcaaattt ggtttggatt atacccttca
     4261 gcatatacaa ttatttctct agtcatactt attagatggc gcaggacaat tgatcgtgaa
     4321 tctccgctta gacggctttt atcaatcttc ttaatttcaa tcatattacc ctttattatc
     4381 ggcatattcc ttggtgtatt cccgcgcttt ttcggtttag aaaacttacc acggctcact
     4441 gttgcatttc tgattccgcc tgcggtttta ttatttatcg cactgcgtaa atttggaatg
     4501 gtattcgaaa caaaaacaag gcgtgacttc tcaccccttg atcccaagac aacataaaaa
     4561 gaaagccgct tgcgcttatt ccaaacagca gcgctaattt tcgggattgg tgccgctgca
     4621 tcatttttct ccggttattt cttaacaaat ggcgatttca ttagggaaat tattctatct
     4681 gtttctattt ggttctttgc tatattcttt ctatttgtgc cacatattac gaagaagatt
     4741 cttattcaaa atattctctt tttcatcact agtgtagttg gtatgacctt cttcatcgtt
     4801 aaagatgcgc atatgggggc atcaacagtg tgggcagttt atattgtctt cttactatat
     4861 tcaattgttc ttgatagtaa acgcgacaca ttattcttct taatttttac agttttcaca
     4921 cagattgtgt tatggattac atttcctaat gttactattg tccttgataa tgcgcagtat
     4981 gctaagcggc tatttattat catactttca tacgtggctg tgcgttatgt tactagtgaa
     5041 tatgattcaa agtttcaagg ttatcaaaga ttctcgaggg gacaagaaat tcttgaaaaa
     5101 atttccacaa atttcatcgc tgttaatcaa gacaatgtcc gagctaaaat tgatgaaatg
     5161 tttatgatgg ccgccgaaat attacagtat gatcaagctt atttgtttcg atttgatgac
     5221 aaaggcgaaa gagcaacaat tcttaatgca tatgtaagag atagtgagga caattcactt
     5281 cttctgcagt taggacgttc ttttaaaatc agcgagtttc cacaagtaga accgctcgca
     5341 ggtaaacatt caccaatctt ctgtgatgat attgcaaatt taggtactca tacacaagat
     5401 gaaagtgcca atttcttcct gtctagaggc attaattctt tctatgcttt accaatgttc
     5461 gttgaagaaa aactaagtgg tttctttgtt gttgaatata aagaaaaaaa cgaactaacc
     5521 gttgtcgaaa gtcgtttaca ctatttaaaa attattgcta atgtattagg tgatgcacgc
     5581 aagaaaataa tttatgaaaa acaactatat gattttgcgt atattgataa atcaactaat
     5641 ttaccaaacc gcaatatgct tattaataat ttagaagagc tattacttaa tcgcgatgac
     5701 gctgataagt tagtcgtctt taatgtggaa cttgataatc tacggatgat taatgataca
     5761 ttcggacacg caacaggtga gcaagttgta attaaatccg catcaattct tcaaacgctt
     5821 atgaaagatg ggtgcgtgct ttcgcgtatt gccgaagata aatttattat cgttatgcca
     5881 attgccgaaa ctatcgccca agtaatggat tgcgctaacg agattacgga tgcttttgta
     5941 aacccaatat taccagcaga tagtattggc tcgctatttg tgacaacaat tgttggtgtt
     6001 gccacattcc ctgaagatgg gagagatgct acaccctttt agaaaatgcc gatattgctg
     6061 cctatgaagc gaaaatatcc gatagtaaag tagtctttag taccgaagaa cttaaaacac
     6121 atattgaaga atatacacta ctaactaata aactttttag ttctttaaaa aataaagagt
     6181 ttaatttaca attccaacca caaattaatt gtaataacgg acaaataaat ggtgttgagt
     6241 cattacttag atggacgact gacgaagggc aaagagtggg accaaatgtt tttatcccaa
     6301 tgcttgaaca aactggctta atccacgaag tcgggttatg ggtcattgac caatcactta
     6361 aacagcataa ggcactagtg aaaaagggat ttgccccact gcgcttttcc gttaatctct
     6421 ctgtagcgca attccgccgt gatgactttg tggataatgt attgaaatta cttaaaaaac
     6481 ataaggtgga acctcagtat cttgaactag aaattactga aagtttgctc tcgaaaaatt
     6541 ttgccgacac tattgaaaaa ttaacaagac ttaatgatgc gggattacac attgccattg
     6601 acgattttgg taaaggatat tcttcactac accgcttaca acttgtccca tttaaccgaa
     6661 ttaaaattga taaaagcgtc gttgataata ttacaatcga accgaagaaa gttgttattg
     6721 ctaaaacaat tattaattta gcgaaatcat taaacgctaa tattacaact gaaggtgttg
     6781 aaaccaaaga acaggcagcg tttatgaaag atttaggcac tgatgaaatt caaggctact
     6841 actactactc acgcccactt ccacctaaag atcttgaaga ctttttaaaa caaaaataat
     6901 cacttacttt taattttata tataatgcac taattaatta ttgcttatta attattaagt
     6961 tgctctttat ctttttttct tcttttaata ttaataatga ttacggcact agaaattgta
     7021 taaagcacgc atgataaaag cattgatagt gaagtaattg ttattgttaa agttgacgct
     7081 acatctgtta ttccatacca aataacatgt aatgacatca ttacatagat aagaaatgtc
     7141 gctactttgc catgccactc agcgctatga acttcaccag tatatctaaa cactacaaag
     7201 cgaagagcga attgaagtat ttcctttaat aacattatgc taattggaat ccacatcagc
     7261 gggaatctag tcactaaaca aaatagaacc gctaattggg ttaatttatc ggcaacaggg
     7321 tcgatgaatt tcccaaaatc agtcaccata tttaatttac gggcaactag accatcaata
     7381 atatcagtga ctagtgaaat gataagtaca acaagcgtcc agccataaag ttctttacca
     7441 acatagagaa agacgataag cggaataagt agtaagcgga aaaaggataa gatattagga
     7501 atggtaaaaa cttgatgagg tgttaattta ctcctaatac taactttgtg catagttttc
     7561 tcctatttcc tttatattta cactaattaa aataattaaa attggtgcag ccaaagggac
     7621 ttgaaccccc acatcttgcg atacaagcgt ctgaaacttg cgcgtctacc aattccgcca
     7681 tgactgcctg cactttattt taacactgca aaaaatttat tcttcaataa tgataatagg
     7741 ttcactaact tttattttac cgtcgttaac aacgatatca gtgttcgtca gcacattagt
     7801 gtaacttccg tcttttaact ctgttttaac ttctttttct ttaagcccta aattaaagaa
     7861 accgtgtgcc gctccaactc ttgactcaag cgttgaaaca attacatcat ctgcaccgtc
     7921 gccaataata ttaaaggaag aaatctcggc aaaaactggg tgtttcttca tttcaacaag
     7981 taaaatcata aagtcataat agtcttggtc tttaacattt aaatcaattt tatcaatgtc
     8041 aaatagtgtc gggcgatgag tagccttaac ttcttgacca ccataaataa accctacacc
     8101 attttggaaa aatgaccaag cagtgacatt acgcaaaatt tgatctgttt tacataaaga
     8161 agcaatccgc acggtatcat gattttcaat cgttctaatt tttaaataac cttttggata
     8221 gttttgctct tgataacgaa cgccatcaag ataaggttta agtggtgcct taccttctaa
     8281 ataggctttt aagtcattat agacatcata gtcataaaga atatcaaaag cactatacat
     8341 ttctttatcg tgatgagcaa catgacctgc tgccgttaaa tttgtaataa aatgcggttc
     8401 cactgattca ctaagccaaa taagttcagg atagtcttca tctagttcct gccgtgcata
     8461 ttcccaaaat gcaaggggaa cgagtggcgc cacatcagcg cgataaccat ctactcctaa
     8521 actagcccag tatttgagca tttctaccat ataatcccat aagtcacgat tattatagtc
     8581 taagtcaata atatcactcc agtcgccaac tttgttgcca agtttgccgt ttttatagta
     8641 ataaaattca ggatgctccg ctaataactc ggcatcagga ctagtgtgat gaagcacgat
     8701 atcaatcatt actttaaaac cgtatgaatg cgctaaagta atgaaagttt gaaagtcttc
     8761 aagtgtaccg tgttccggat taattaaata gtaatcacga atcgcataag gacttcctag
     8821 ctcaccttta cgagcaattt caccaatagg gtgaattggc agcaagtata aaatgtcgac
     8881 accaagctcc tttattcttg gtaggtcctt aattaaagcg acaaatgttc cttctgcggt
     8941 gtgattacgc acataaattt gataaattgt ttggttgcgt aaatatgttt ttcttttcat
     9001 actttcattt tagcaaataa acagctttaa gtaataaaaa aactcgatat ctagaggggg
     9061 atctaaatat cgagattttt acaataatgg tggagccgac gaggatcgaa ctcgctacct
     9121 cctccatgcc atggaggcgc tctcccaggt gagctacggc ccctaattgc ttgtttataa
     9181 taatactatt tgcataagtt gtcaatcaat ttcactatta aggacattta tataaatagc
     9241 aagaacgcca aaccggtcaa tatcgtcctt tttataatac tcttccatat cactaggcgc
     9301 aacttcttca ccatcgagat aacctaattc tttgggagta taagctgcgt aaagttcagt
     9361 gaaatttaga tagcgagtta attccaatat ttctactttt aaaatttctt tcgtctctgt
     9421 attagtaaaa taaataaagt cgcctacgcg aagcttttgg cgcttttcat cataaaggcg
     9481 catttctata actttttgtt tatttttaat gcgcataaat ggaattggtt ttaaagtaaa
     9541 ttcatgtctt ttcataaaaa ggctggcgca ctggggagga ttcaaacctc cgacctaccg
     9601 cttaggaggc gattgctcta tccactgagc taccagcgcg catacttaat
//
LOCUS       DAOSTA010000034         9212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_7186_length_9212_cov_9.064978, whole genome shotgun sequence.
ACCESSION   DAOSTA010000034 DAOSTA010000000
VERSION     DAOSTA010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7186_length_9212_cov_9.064978"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1336)
                     /locus_tag="PLU08_03895"
     CDS             complement(206..1336)
                     /locus_tag="PLU08_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOQ70864.1"
                     /translation="MREAYKKIPCRITVIDLLKLTGMIFICIPHCLQRWGPSDYTSTL
                     GFSFFYSVGLSMFFFSSGIVLRRITSLKDLLIFIVKTVGVYLTSAFIFTVLSIYTLPR
                     FTLLEKDFGYWMNELYLRTDTFYWYLLVATLINIFIASAYYFVSLIKKNNLKWDIFKA
                     SLIIILTVAYSMIFLHIYNREDLGPGCLGASLVLYYLPITLFGFLFNIFKPYLLKLPR
                     QELLKGIATLVALVIYIPLLIVYRDWLPHLNGSFWTISALWICSLSGTIVYYYLLSKI
                     SHFTIAKKVSCYGFFSGQFYLVHVYIIRLFATYIPRPETFDFPAIMFVTFLTIIFTIG
                     SILITVFLRFIPYTDFLLFISYDRYKDIIYPHRWLKRVVLQK"
     gene            1474..2481
                     /locus_tag="PLU08_03900"
     CDS             1474..2481
                     /locus_tag="PLU08_03900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOQ70865.1"
                     /translation="MRILLFNKKQKLYDKSGVGRAMKHQVKALTEAKIPFTTNEKDDY
                     DLVVINTIDPSAFRMARKARRQGKKVIVYGHSTKEDFMNSFMLSNQIAPFFKIHLKRI
                     YKMGDLVITPTPYSKKLLDTYGLNREVVALSNGVDLARFNADNKEKIQKFREYFKLKP
                     DQKVVMTVGLFLARKGLHDLIEIAKDRPEYTFIWFGHTPKAVLSRKIKKAIKNKPDNV
                     IMPGYIAGDIIEGAFANADVFFFPTYEETEGIVVLEALASKCPLLIRDIPIYEEWLTH
                     GVNCHKGKTNEEFKALLDDIITKKAADTREAGYKVAAERDIKEIGKKLGEIYRRVLEE
                     K"
     gene            complement(2507..3349)
                     /locus_tag="PLU08_03905"
     CDS             complement(2507..3349)
                     /locus_tag="PLU08_03905"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HOQ70866.1"
                     /translation="MNDLQKVLLENLKEFIRVCEKHNLRYYLVTGSALGAVRHKGFIP
                     WDDDIDVAMPRNDAMKLLALKDEFKEPYFLQHYTTDPSFPYPFMKLRNSATTFEEGLL
                     KFHKINHGIYIDIFIIDGMSKTSTLKNKWKNALMWPVMYLIYAANFWQKPRKNTFILQ
                     ILLYLVSILLLPFKINNWLIKALDKSMAKIPFEEATLVGAYLLWGGNKEAMDKRIYGP
                     GVKMKFETIEAVLPENYDAYLINRYGNYMELPPEHKRVGHHKPSALSTKVSYKDYYKR
                     KKRR"
     gene            complement(3370..4257)
                     /locus_tag="PLU08_03910"
     CDS             complement(3370..4257)
                     /locus_tag="PLU08_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HOQ70867.1"
                     /translation="MNKKQLEIMRSGKGFIAALDQSGGSTPKALRTYGVPETAYKTDE
                     EMFTKVHEMRTRIIKSDAFNSERILGAILFENTMDRKIDASYTADYLWEEKGIVPFLK
                     IDQGLCEPISGVQLMKPMPNLASLLERAKERKIFGTKMRSVIKEANEQGIKLIVEQQF
                     AVAKKVINYGLVPIIEPEVDINSPTKKEAETILLKFILEELAKLGPNDLVMFKVTLPT
                     MMNLYAPLLKDPRVVRVVALSGGYTLEHANDLLYLNKGVIASFSRALVADLRVDQSDK
                     DFDKTLAKAVESIYEASLT"
     gene            complement(4348..4959)
                     /locus_tag="PLU08_03915"
     CDS             complement(4348..4959)
                     /locus_tag="PLU08_03915"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOQ70868.1"
                     /translation="MVRKEIKQQAKDLIKNGKWFLLFVVLLIVSAVSSLTAGILMPVA
                     LFGLHLAIVGMFAGQEFDFNHFGTPFNDFNHALKVIAVYFLTMLIVMVGTMLFIIPGI
                     IFYMMYVQAYRIMIEKPEIGIMDALRESKEMMNGYKMDYFVFQLSFIGHFLLIMITFG
                     LWVIYLMPYFVVADTNYYLHLKQVRNEAKVVAEAAPVEAEVVE"
     gene            5127..6287
                     /locus_tag="PLU08_03920"
     CDS             5127..6287
                     /locus_tag="PLU08_03920"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOQ70869.1"
                     /translation="MTIKDHIKHRFAAIYNTPPVAVYFSPGRVNLIGEHIDYHGGLVL
                     PVALSIGTYGAYSLRDDDEVRLYSTGYSDYPITINLGDLSKDSFTGWANYVRGVFQVL
                     KDEGYHVPHGLNLYLESDMPTSGGLSSSSSLELLIIKMLNDIFNFGIDRTKMAVLGKK
                     VENEYIGVLTGIMDQFVIANGKKNNALLLNTNTLKFDYIPLDLKDYQLVIVNTNKRRG
                     LADSKYNERWNETMGALKTLKNHFKINNLTELKSSDLPQIEKLLDPLLFRRVRHIITE
                     QERTLESAMLLKEGKIEEFAKLLTASHNSLRDDYEVTGLELDTIVLLLLETGAIGARM
                     TGAGFGGCAIAIVPKEKVEKQAPLVIDLYKKVIGYAPSFFASDSSDGTHELK"
     gene            complement(6279..7241)
                     /locus_tag="PLU08_03925"
     CDS             complement(6279..7241)
                     /locus_tag="PLU08_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70870.1"
                     /translation="MKLYVLENDLLKVTFLDVGASIYSFSVKALDNRNIVLTTKDKNI
                     YKEPPKNAYFGATVGRVGGRIANGEFIIDGKKYQAAQNEKVTNSLHGGFESYAAKLFE
                     VVEEKEDYITFRYVSRDGEGGYPGELELYVKYTLLDNCLALDYEARTTKKTLVNIINH
                     SYFNLNGKGDILKHKVSADVEAVFALDDKQINLLPLKVRKGDTFDLSTGKKLEKMVFA
                     KEVNVPPMGGVDHLLVTPKRKLTLEGEDLRLIVLSDYPAVQIYSTNYPPEVTLLEGTK
                     VIKYGGLAIEPADIVCGQNGEFKNLELCPGEVYKRRIKYIVELL"
     gene            complement(7485..>9212)
                     /locus_tag="PLU08_03930"
     CDS             complement(7485..>9212)
                     /locus_tag="PLU08_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70871.1"
                     /translation="KTPSSEKPPVSSEQPPVTSVTTEEPPVSSEQPSEPPVSSEEPPS
                     SEVEPGEPIKLGDAAAALADPNEIYLQVGEKVNLTYARIVDNVPRIQFTVEDGVAYDA
                     LKLFVKFPGEVGKRYKVNAAFTAAAAFTGTINGERFDFIRGENNLEFYYDELENEVSI
                     AFVFGFNEEGLGLKANNITFGEGSISERVYDDPETTLAVDGDISDWSNLKAFENTVGV
                     HSNTAEVKHKSVDFYASLTSTGLYLAAEAYHDILIEESEHWWQSTNFEIFIQGNNQYW
                     VSARKVDGAFTKRDNVTEAAMVTTPLTGEGVANFRTVAEMFIANANLPEAAVVGGEIR
                     VGFAWKTDGDLLDNGEAAGGGLDCYWVPKGTWTDNADQTFVTINGIFKQTQLTFEPTT
                     MTIDGDLTDWEEKAAYTTNKIYIEGSDATNHKNVTFMAFLNDEGLFLAALAHHDVLIN
                     NEEIWHSNTNFEFFLNGGNQYYVTAAGQVSYGKGIIVTQAYKGEPYAEYESVAEFFIP
                     RWALPEGDMHRVGFAWKTNGDTATGLGGSGGGPDAWWFEAGHFPNNADEQYYVTAEGL
                     FRTDPTPAP"
BASE COUNT         2985 a         1757 c         1577 g         2893 t
ORIGIN      
        1 tctattttta aaactttatt ttctattttg tcactttatc tttttcatgt aacaaaaatg
       61 ttttagaatt acactttttg taatttatga ttatttattt tgcaaagtaa aataaacaca
      121 gcaaattttt attcacggtt gaaacatttt ttcattatta attgacgact atcagcgtag
      181 cacttcttaa ttaaatcatt aaattttatt tttggagcac tactcttttt agccaacgat
      241 gcggataaat aatgtcttta tagcgatcat aactaataaa taataagaag tccgtgtaag
      301 gaataaagcg taagaaaaca gtaattaaaa tactaccgat tgtgaagata attgttaaaa
      361 aggtaacaaa cataattgcc ggaaagtcaa acgtctcggg tcgcggaata tatgtagcaa
      421 aaagacgaat aatatacaca tgcactaaat aaaattgtcc gctaaagaag ccgtaacatg
      481 atactttttt agcaattgta aagtgtgaga ttttggaaag taagtaataa tacacaattg
      541 tcccgctaag tgaacatatc cataaggcac taattgtcca aaatgagccg tttaaatgag
      601 ggagccaatc gcgatacaca attaaaagcg gtatataaat tactagcgca actaaagtag
      661 cgataccctt aagtaattct tggcgtggta gtttaagtaa atacggttta aatatgttaa
      721 aaagaaaacc aaataaagtt atcggtaaat agtaaagcac taaagaagca cctaagcacc
      781 ctggtcctaa gtcttctcta ttataaatat gtaaaaaaat cattgagtaa gcaactgtaa
      841 gtataattat aagacttgcc ttaaaaatgt cccacttaag gttatttttc tttattaaag
      901 acacaaagta atatgcacta gcgataaaaa tattaattaa tgttgcgaca agcaaatacc
      961 aataaaatgt atcagtgcgt aaatacaatt cattcatcca gtagccaaag tctttttcta
     1021 aaagcgtaaa gcgcggcaaa gtataaattg ataaaacagt aaaaataaag gcggatgtta
     1081 aatacacacc aacagttttt actataaata taagcaaatc tttaagtgat gtgatgcggc
     1141 ggagcacaat tccgctacta aagaaaaaca tcgaaagacc aacactatag aaaaaagaaa
     1201 agcctaatgt tgatgtataa tcacttggcc cccatctttg taaacagtgc gggatacaaa
     1261 taaaaatcat ccctgtaagt tttaataagt caatgaccgt tatccgacac ggtatctttt
     1321 tgtaagcttc acgcatagct ttaactcttt ctattattaa gggtttagtt ccctttaata
     1381 gcaataatta tagcattaaa ttataaacta ttaacacgtt caaaaaagta tatatcgtga
     1441 tatgataaaa agagagtgaa aggagcccgg agtatgcgaa ttctcttatt taataaaaaa
     1501 caaaaattat atgataagtc tggtgtgggt cgcgcgatga agcatcaagt caaagcatta
     1561 acagaagcaa aaatcccgtt taccacaaat gaaaaagatg actatgatct cgtcgtcatt
     1621 aacaccattg accctagtgc tttccgtatg gcacgcaaag caagaagaca aggaaaaaaa
     1681 gtaatagttt atggtcactc aacaaaagaa gactttatga actccttcat gctaagtaat
     1741 caaattgcac cttttttcaa aatacactta aaacgcattt acaaaatggg tgaccttgtc
     1801 ataacaccaa caccatactc caaaaaatta cttgatacat acggcttaaa tcgtgaagtt
     1861 gttgccttat caaacggtgt cgatttagcg agatttaacg ctgataataa agaaaaaatt
     1921 caaaagtttc gtgaatattt taagcttaaa cctgaccaaa aagtcgtaat gactgttggg
     1981 cttttcttag cgcgtaaagg tctccacgac ttaattgaaa ttgcaaaaga tcggcctgaa
     2041 tatacattta tttggtttgg acatacccct aaagcggttc taagtcgtaa aattaaaaaa
     2101 gccattaaaa ataaacccga taatgtaatt atgccaggct acattgctgg tgatattatc
     2161 gaaggcgctt ttgcaaacgc tgatgtcttc ttcttcccaa cttacgaaga aacagaagga
     2221 attgttgtcc ttgaagccct cgcaagtaag tgtccacttc tcatccgtga tatcccgatt
     2281 tacgaagaat ggttaacgca cggtgtcaat tgtcataaag gtaaaactaa tgaagaattt
     2341 aaagcgttac ttgatgacat tattactaaa aaagccgcgg atacaaggga agcaggttac
     2401 aaagtagcag cagaacgtga cattaaagaa atcggtaaaa aactcggaga aatttatcgc
     2461 cgagtactcg aagaaaaata gttaactaaa cgacaaaaaa ggcgccctag cggcgctttt
     2521 ttcgtttgta ataatcttta taactaactt tagtgcttaa ggcgctcggt ttatggtgac
     2581 cgactctttt atgttctggt ggtaattcca tgtaattacc ataacgattt attaaataag
     2641 catcataatt ttcagggaga acggcttcaa ttgtttcaaa tttcatctta acacctggac
     2701 cataaatccg tttatccatc gcctctttat tcccgcccca aagcaggtaa gcaccaacga
     2761 gtgtcgcctc ttcaaaaggg attttagcca tacttttatc aagtgcttta atgagccaat
     2821 tattaatttt aaatggtaat aaaagaattg atacaagata gagtaagatt tgaaggataa
     2881 atgtattttt acgcggtttt tgccagaaat tcgcggcata aataagatac atcactggcc
     2941 acattaaagc gtttttccat ttatttttaa gggttgatgt cttgctcatc ccatcaataa
     3001 taaaaatatc aatataaata ccgtgattga tcttatgaaa ctttaaaaga ccttcttcaa
     3061 aagttgtcgc gctattgcgt aacttcataa acggataagg aaaactgggg tcagttgtgt
     3121 agtgctgtaa gaagtatggt tctttaaatt catcttttag cgcgagaagt ttcatcgcat
     3181 catttcgtgg catcgctaca tcgatatcat catcccacgg aataaagcct ttatgtctta
     3241 cggcacctag tgcactccct gttactaagt aatagcgtaa attatgtttt tcacaaacac
     3301 gaataaactc ttttagattt tctaataata ctttttgtaa atcattcatc cttaatgtct
     3361 tttccttatt taggtaagcg aagcttcgta aattgattca acggccttcg ctaaagtttt
     3421 gtcaaagtct ttatcacttt ggtcgacgcg taaatccgcg actaatgccc gtgagaaact
     3481 agcaattaca cctttattta aataaagtaa atcattagcg tgctcaagag tataaccccc
     3541 gcttaaagct acaacacgaa caacacgcgg atcttttaaa agtggtgcat ataaattcat
     3601 cattgtaggt agagtcactt taaacatcac taaatcattt ggtcctaatt ttgctaactc
     3661 ttcgaggata aattttagaa gaatcgtttc tgcttctttt ttcgttggac tattaatatc
     3721 cacttcaggt tcaataattg gcactaaacc gtaatttatt acttttttag cgaccgcaaa
     3781 ttgttgttcg acaataagtt taatgccttg ttcattcgct tctttaatga ccgaacgcat
     3841 tttagtacca aatatttttc gttctttcgc tctttctaaa agactagcaa gattcggcat
     3901 tggcttcatc agttggacac cacttatagg ttcacaaaga ccttgatcaa ttttaagaaa
     3961 aggaacaata cctttttctt cccataagta atcagcggta taacttgcat caatcttccg
     4021 gtccattgta ttttcaaata agattgcccc taaaattcgc tcactattaa aggcatcgct
     4081 ttttataatt cgcgtccgca tttcgtggac cttagtaaac atttcttcgt cagttttata
     4141 tgcggtttca gggacaccat aggtgcgtaa tgcttttggt gttgaacctc cgctttgatc
     4201 aagagcggca atgaaccctt tcccgcttct cataatttct aattgttttt tattcataat
     4261 aaaaactccc ctctatctat acatattgta aaggaaaatt attaaattac atatgtttta
     4321 gcacaggaga gttttaattt ttaaaaatta ttcgactact tcagcttcaa ctggtgcagc
     4381 ttcggcgacg acttttgctt cattacgaac ttgtttaagg tgtaagtaat aattagtgtc
     4441 ggcaacaaca aagtatggca ttaaataaat tacccatagc ccaaaggtaa tcattattaa
     4501 taagaagtga ccgatgaaac taagttggaa cacaaaataa tccattttgt agccattcat
     4561 catttcttta ctttcacgaa gcgcatccat aataccaatt tctggttttt caatcatgat
     4621 acgataggct tggacataca tcatatagaa gatgatgcca ggaatgataa ataacattgt
     4681 acctaccatc acgatgagca ttgttaagaa ataaaccgcg ataactttta atgcgtggtt
     4741 aaaatcatta aatggtgtgc cgaaatgatt aaagtcaaat tcttgaccgg caaacatacc
     4801 tacaattgct aagtgtaatc caaagagtgc gactggcatt aaaataccag cggttaaact
     4861 tgaaactgca ctaacaatta agagaacgac aaagagtaaa aaccatttcc cgttcttaat
     4921 taggtctttt gcttgttgtt taatttcttt tcttaccata aattatcctc cttcttaaat
     4981 tataacctat ccaataattt aacagtagtt aaaattcaat tttttgtgat tacttgataa
     5041 ttattactcc tagagtaatt aaaaatgaaa tagggtaaaa tgtatttgat atatacttaa
     5101 aatctcatca caaaggagac aaacttatga caattaaaga tcatattaaa catcgctttg
     5161 ccgctattta taacactcca ccagttgcgg tatatttttc accaggaaga gttaatttaa
     5221 ttggtgaaca tattgattat catggcgggc ttgtacttcc tgtagcactc tcaattggga
     5281 cgtacggggc atatagcttg cgtgatgacg acgaagttcg cctttattca actggctata
     5341 gtgattaccc aatcacaatt aatcttgggg acttatcaaa agactccttt actggttggg
     5401 caaattatgt gcgcggtgtg tttcaagttt taaaagatga aggttatcat gtgccacacg
     5461 gtcttaatct ctatctagaa agtgacatgc ccacaagtgg cggactatca tcttcttcat
     5521 cacttgaact gcttattatt aaaatgctta atgatatatt taatttcggt attgatcgca
     5581 caaaaatggc cgtgttagga aaaaaagttg aaaacgaata cattggtgtt ttaacaggga
     5641 ttatggacca gttcgttatc gctaacggga aaaagaataa tgcactgctc cttaacacaa
     5701 acacgctaaa gtttgattat atcccgcttg acctcaaaga ttatcaactt gtcattgtta
     5761 atacgaataa gcggcgcggt ttagcggatt ctaaatacaa tgaacgctgg aatgaaacaa
     5821 tgggagcact taaaacatta aagaatcatt ttaaaataaa taatttaaca gaacttaaga
     5881 gcagtgattt accacaaatt gaaaaactgc ttgacccgct tctatttcgc agagttcgcc
     5941 atattattac cgaacaagaa cggacactag aaagcgctat gcttttgaaa gaggggaaaa
     6001 ttgaagaatt tgccaagctt ttaaccgctt cacataattc gttacgcgat gattacgaag
     6061 taacagggct tgaacttgat accattgttc ttctcctttt agaaacagga gcaatcggag
     6121 ccaggatgac tggtgccggc tttggcggat gcgcaattgc gattgtgcca aaagaaaaag
     6181 tggagaagca agctccactt gtcattgatt tatataagaa agttataggt tatgcaccgt
     6241 cattcttcgc tagtgatagt agtgatggca ctcatgaatt aaagtaattc aacaatatat
     6301 ttaatacggc gcttataaac ttcacctgga catagttcca ggtttttaaa ttcaccattt
     6361 tgcccgcaga caatatccgc tggttcaatt gctaacccgc catattttat aacttttgtg
     6421 ccttcaagta gtgttacttc cggcggatag tttgtactat aaatttgcac tgctggataa
     6481 tcactaagaa ctattaagcg taagtcttca ccttcaagcg ttaatttccg ctttggagta
     6541 acaagtaaat ggtcaacccc gcccatcggt ggtacattta cttctttggc aaacaccatt
     6601 ttttctaact ttttaccagt gcttaagtca aaagtgtcgc cttttcttac tttaagaggc
     6661 agtaagttaa tttgtttatc gtcaagcgca aatactgctt caacatcagc gctaacctta
     6721 tgttttaaaa tgtcaccttt accatttaaa ttaaagtaac tatgattaat aatgttcact
     6781 aatgttttct ttgttgtccg tgcttcgtaa tcaagcgcta aacagttatc tagtaatgtg
     6841 tatttaacat atagttcaag ttcaccagga taaccccctt caccgtccct acttacataa
     6901 cggaaagtaa tataatcttc cttttcctcc actacttcaa aaagtttagc ggcataactt
     6961 tcaaatccgc cgtgtagtga attagttact ttttcatttt gcgcggcttg atacttcttg
     7021 ccatcaatta taaattcacc gttagcaatc ctgccaccaa cccgcccaac agtagcgcca
     7081 aaataagcat tcttcggtgg ttctttataa atgtttttgt cttttgttgt taagacaata
     7141 ttgcggttat ctagcgcctt aacactaaag gaataaatac tagcaccgac atctaaaaaa
     7201 gttactttaa gcaagtcatt ttctaaaaca tataatttca taaaattatc cttttccatt
     7261 gtataaatac attttaatta tacataaaat aagtatgttt tgcagtaata aatgctgatt
     7321 ttgccactaa ataatagaaa aggtcctatt aatgatataa ctaggaccaa attcttaatt
     7381 acgagcattt tgtaaacaag gtctcctcgc acggtgtgtg cgaggagata gagataataa
     7441 tgaaattatc tgagtattat ttatgtctaa actttattgt ttaattatgg agcaggtgtt
     7501 ggatcagttc taaagagacc ttcagcggta acatagtatt gttcgtcagc attatttggg
     7561 aagtgtccag cttcaaacca ccacgcatct ggtccaccac cactgccacc taaaccagtt
     7621 gccgtgtcgc cattagtctt ccacgcaaag ccaacgcggt gcatatcacc ttctgggagt
     7681 gcccagcgcg gaataaagaa ttcggccact gattcatatt ctgcatacgg ttcgcctttg
     7741 tatgcttgtg tcacaataat acctttacca tagcttactt gccctgctgc ggtaacataa
     7801 tactgatttc caccattaag gaagaattca aagttcgtat tagagtgcca tatttcttca
     7861 ttattaatta agacatcatg gtgcgctaaa gcggcaagga agagtccttc atcatttaag
     7921 aaagccatga atgtaacatt cttatggttt gttgcatcac taccttcaat ataaattttg
     7981 ttagtagtgt aagcagcttt ttcttcccaa tcagttaagt caccatcaat tgtcatggtc
     8041 gttggttcaa atgttaattg tgtttgcttg aaaatcccgt taatcgtcac gaatgtttgg
     8101 tcagcattat ccgtccaagt accttttggt acccagtaac agtcaagacc accaccagca
     8161 gcttcaccgt tatctaataa gtcaccatct gtcttccaag caaaaccaac acggatttca
     8221 ccaccaacaa ccgcggcttc tggtaagtta gcgttagcaa taaacatttc agcaactgta
     8281 cggaaattag cgacaccttc gcccgttaat ggggttgtca ccattgcggc ttctgtaaca
     8341 ttatcacgtt tagtgaatgc accatcaact ttgcgcgcac taacccagta ttggttatta
     8401 ccctgaataa agatttcaaa gttagtgctt tgccaccagt gttcactttc ttcaatgaga
     8461 atatcgtgat atgcttcggc ggctaagtat aacccagtgc ttgtcaagct tgcatagaag
     8521 tcgacactct tgtgcttgac ttctgcggtg ttactatgga caccaacggt gttttcaaaa
     8581 gctttgagat tactccaatc gctgatatca ccatcaacag caagcgttgt ttctggatca
     8641 tcataaacgc gttcggaaat actaccttcg ccaaaagtaa tattgttggc ttttaggcct
     8701 aaaccttctt cattaaagcc aaagacaaag gcaatgctaa cttcattttc tagttcatcg
     8761 taataaaatt ctaaattgtt ttcaccacga ataaaatcga agcgttcgcc attaattgtg
     8821 ccagtaaagg ccgcagcagc agtaaatgcc gcattaacct tatagcgttt acctacttcg
     8881 ccagggaatt tgacgaataa tttaagtgcg tcataagcga caccatcttc aacagtaaat
     8941 tggatgcgag gtacattatc aacaattcga gcatatgtga gatttacctt ttcaccaact
     9001 tgtaaataaa tttcattagg atcggcaaga gcagcagcag cgtcccctaa tttgattggc
     9061 tcacctggtt caacttctga gcttggtggc tcttcagagc ttaccggtgg ctcacttggc
     9121 tgttctgaag atactggtgg ctcttcagtc gtcacacttg ttacaggtgg ctgttccgaa
     9181 cttactggcg gtttttcact tgaaggtgtt tt
//
LOCUS       DAOSTA010000035         9123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_7255_length_9123_cov_12.504521, whole genome shotgun sequence.
ACCESSION   DAOSTA010000035 DAOSTA010000000
VERSION     DAOSTA010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 9123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9123
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7255_length_9123_cov_12.504521"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PLU08_03935"
     CDS             <1..909
                     /locus_tag="PLU08_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70872.1"
                     /translation="TMKMNSLSRTHQLVLGALMGAINVIFALISSYLFAFSLIIMLFL
                     PLASIIVAINIDLKFYPVYLLGTLTLALVLNLGNIDNTLFFLLPILTSGLAFGLLIRH
                     KVPDILILLIVSGVNFLTLLITIPIINLIYDVNFLQVFASFIGFNNIEFGELVLPSIL
                     TLLAVMQTLITLVIVTQDAAYFRLEINTEEWPYISLVNLGFSAIVTVLMFFNHGISLA
                     LLFVVILLSLYQIVHLFQKHTIFAWSTLLATIVFIIIGLALFENYTSLPYYFGIIIAV
                     IPVVISDILWLYISRKKSEAKNEGTI"
     gene            893..1318
                     /locus_tag="PLU08_03940"
     CDS             893..1318
                     /locus_tag="PLU08_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70873.1"
                     /translation="MKGLFKRLGQGILFILALPFGIAIFILFSIYAIITFFYTFFSAI
                     PAFFKGENVFGPTELDIAASTRLAEQKLAAQQPPPPPVVQPSTTINLFTVQQPGTEQV
                     PPPQVTYTQSDGVTYRRVTEQEVAALPEETIIDNSEEQK"
     gene            1315..2034
                     /locus_tag="PLU08_03945"
     CDS             1315..2034
                     /locus_tag="PLU08_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70874.1"
                     /translation="MILNPYILIKLSESEKRFLIVLFFLIVVVLFVCGLFYEGITRFM
                     ARQGEDIGRLMATQVESGYISSLKQFKRNSYRKSRLEFYRQFIKTFIMMIIWFAIYGT
                     VSIFYRHWLDLFDYTSEGFTTLLWKFDYANTPRTTFFGMSIISDWPPLLHKPFFSAKA
                     IPSYLLAVFGIVIIILFVGQVLSYLARTIFIIQHSKKMFTRDLRDYKLSDLSAAPAEL
                     QESGVNIPEVKPKEPLPPTNG"
     gene            complement(2024..2176)
                     /locus_tag="PLU08_03950"
     CDS             complement(2024..2176)
                     /locus_tag="PLU08_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70875.1"
                     /translation="MNTNKYGQDIPLTKEEIAIRGYKRRNLVLGIIIIILIIIIGIQI
                     GVMLTR"
     gene            complement(2186..3553)
                     /gene="asnS"
                     /locus_tag="PLU08_03955"
     CDS             complement(2186..3553)
                     /gene="asnS"
                     /locus_tag="PLU08_03955"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOQ70876.1"
                     /translation="MLNVKALYTAYEKGELKAIPVKLQGWVKTNRNNGTIGFIEFNDG
                     SAFKGVQIVYEKNKIDNFAEVDKYRTGAALEVYGKVVLTPEMKQPFEVHADKVVLVGD
                     VAEDYPLQKKRHTPEFLREIAHLRPRTNTHTALFRLRSELSYALHKYLYDHEFVYVHT
                     PIITGNDAEGAGNVFNVVTDHKDPNFFFGKEASLTVSGQLHVESFALTFGKVYTFGPT
                     FRAEESNTTRHAAEFWMLEPELAFTDLNGNMDSIEEIIKYSVAYVLEKCSDEIDFFTK
                     FYDKTLKDRLEALVKSKVLRITYTKAVEILKEAQQKGVKFQFDNIFWGMDLASEHERY
                     LCEVYTNGPVFVTDYPKDIKAFYMRLNDDGKTVAACDLLVPGVGELVGGSQREDRYDV
                     LKAKMIEGGNLEDLEWYLDLRKYGGVPHSGYGIGFDRLLMYLTGVQNIRDVQPYARVF
                     GSIKY"
     gene            complement(3589..4602)
                     /gene="tsaD"
                     /locus_tag="PLU08_03960"
     CDS             complement(3589..4602)
                     /gene="tsaD"
                     /locus_tag="PLU08_03960"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOQ70877.1"
                     /translation="MKERIILAIESSCDETAVAIMRGDKLLSNAVSTQISMHQKYGGV
                     MPELASRLHTENIVYTLDTALKDANIKLEEVDAFAVTRGPGLIGALHVGLQAAKTLAL
                     LYNKPLIPVHHLAAHIYASEFVKPLVFPLLAIVVSGGNTEFVVMKKHLDFTTIGETRD
                     DAIGECYDKIARVLNLGYPGGIPIDRLAKEGEATYKLPSPALTGYDFSFSGLKTHVIN
                     LVNNTKQRGEEINVPNLCASFQKVAIDAIIKKGLKAAQEYKVKQVVIAGGVSANSYLR
                     SEVLAKFPKIGVDPAIPPLWCCTDQAAMIAKLASYLYDEKVFADYDLGVDPNWEIEDF
                     REF"
     gene            complement(4599..5060)
                     /gene="rimI"
                     /locus_tag="PLU08_03965"
     CDS             complement(4599..5060)
                     /gene="rimI"
                     /locus_tag="PLU08_03965"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOQ70878.1"
                     /translation="METKEKKLIIRPMTIDDVDAVDELAFAALPDPWPKYTYAQELKN
                     EFAYLFVLTYADVILGFSHFWITFDSATIVKFAVHPAAQGKGLGSLLMAHLIERVSML
                     EEVRAITLEVRTSNDKAMHLYLKYGFNVVTKKRKYYEDGEDAYYMIKVLKE"
     gene            complement(5065..5646)
                     /gene="tsaB"
                     /locus_tag="PLU08_03970"
     CDS             complement(5065..5646)
                     /gene="tsaB"
                     /locus_tag="PLU08_03970"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOQ70879.1"
                     /translation="MITLLLDSSSHHLSVALAKGDKVLSFVEEKAFQTQSEIMVGRIN
                     KLLAQEKLTSDDIKRIVVASGPGSFTGVRIAVTIAKVWAYAKEVPLYAVSSLSVYRHE
                     TKPTICVLDARRERSFAGVYAQNNAILPDKIMQNSEILALAEKEGYLISGETKHLGIE
                     PAKFNRFENMLKSISEENRVKDIAAFKPLYLKG"
     gene            complement(5643..6104)
                     /gene="tsaE"
                     /locus_tag="PLU08_03975"
     CDS             complement(5643..6104)
                     /gene="tsaE"
                     /locus_tag="PLU08_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019157052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOQ70880.1"
                     /translation="MKHEFISISREQTIALGKRLAALFNLGDVVLLTGDLGAGKTTIT
                     KGIASGLNITEKVNSPTFNILKIYLKGDKPLFHIDAYRLEDDKKDIGLDEYIGEAGIT
                     VIEWPAFIKHLLPEKALEINITHRDLTSRNITLKGEGHYADVVKVIAEEFK"
     gene            complement(6159..6692)
                     /locus_tag="PLU08_03980"
     CDS             complement(6159..6692)
                     /locus_tag="PLU08_03980"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HOQ70881.1"
                     /translation="MSIKGKWIKIQSYKHDGNLHRFWRKSYVLEDNDEWLIVASRRTQ
                     VIEGNGRKWFTKEPAVTFFHKNDWFNVIAMLKTEGVVYYTNIASPTLIDYGMAKYIDY
                     DLDVKKFSDGTIKYLDKNEFLRNIENYQYSKELSAIVEYKQTQVVAMINEKTFPFDDD
                     EVRKLYNRFVEETKIKK"
     gene            complement(6749..7735)
                     /locus_tag="PLU08_03985"
     CDS             complement(6749..7735)
                     /locus_tag="PLU08_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70882.1"
                     /translation="MTEKEKMVEVVEETPAETKTPQAFVNEEISDDATNEQLELPALE
                     TMRNTFSKMYRKFRIFSYVSTAIVIAVIVVSYVWLLPINQWAGIVPIILVLVASLVYS
                     RWARNKLTDNVRKYMADYNGFVNELALSESKVNNYVFDFAGQLDKAAFTDARLLKDII
                     NSNSRNLVTYDLGRWHVKIADYAAYRRDGKQARAVFLGKLVTGTTEKEIDGRLVLYIK
                     PSEQKYDGGTGPDDLEDLELVVDKPEYKLYATNKELKKAISTKALEALLQVRPDNELA
                     DVSVALYKNKFAVTLTYSDALMVVPYKENVPVHAIKKYKEHLTALNKFIALL"
     gene            complement(7744..8385)
                     /locus_tag="PLU08_03990"
     CDS             complement(7744..8385)
                     /locus_tag="PLU08_03990"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ70883.1"
                     /translation="MMILNYTNKTNQELERLIKSNDPAAMHELARRLVQGEGIAEDNK
                     RGIELARQSEALGYAPADTVIGYCYFYGKGVEEDKQKGFAYFHSGAVKGHDAAQYNMG
                     IAYERGTVIPQDYALALEWYQRAADQKFAKAFYKLGYFHEHGMGVTKDVTKAFSYYEK
                     SANLGYVKAEVEVGKAHLSGRGLLRDLTKAREYLERAAAKGNRIAARLLRDIE"
     gene            complement(8430..9050)
                     /locus_tag="PLU08_03995"
     CDS             complement(8430..9050)
                     /locus_tag="PLU08_03995"
                     /inference="COORDINATES: protein motif:HMM:NF021062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HOQ70884.1"
                     /translation="MKETTKKRRTLKPNRFFGRILTVRGITEMAIFIALALVFDLEFL
                     KFRLGEAQSFSITMLPLMIIALRFPLVDAFIGIGGIYGVITMLWDGYGLITYPLDYLL
                     AYGFLSIISLFQALVYGKHKHEIINYLYLALAVPAGVSGRIFFSTISGVVVYETPFVG
                     SLLYNLPAIGVSGIICGVLLMMLFPTLLRFRPQPFLQNEVEESKQQ"
BASE COUNT         2738 a         1840 c         1428 g         3117 t
ORIGIN      
        1 actatgaaaa tgaactcatt atcacgcaca catcaactcg ttttaggcgc cctcatgggt
       61 gcgataaatg taatatttgc acttatcagc agttatttat ttgcttttag tttaattatt
      121 atgctttttt taccgctggc gagtattatt gtcgcaatta atattgattt aaaattttat
      181 cctgtttatt tactaggtac gcttacctta gcgcttgtat taaatcttgg taatattgat
      241 aacacacttt ttttcttatt accgatatta acaagcggat tagcctttgg gcttttaatt
      301 agacacaaag tacccgacat cttaatttta ttaatcgtta gcggtgtgaa ctttcttaca
      361 cttttaatta caataccgat tattaattta atttatgatg tgaatttctt acaagtgttc
      421 gctagtttta tcggttttaa taatattgaa tttggtgaat tagtgttgcc ttcaatctta
      481 accttattag cggttatgca aacactcatt acgcttgtga ttgtaacgca agacgctgct
      541 tattttagat tagaaattaa tacggaagag tggccttata ttagtttagt taatttagga
      601 tttagcgcta ttgttacggt attaatgttt tttaatcacg ggatttcgct tgcgcttttg
      661 ttcgttgtta ttttgctttc actctatcaa atcgtgcatc ttttccaaaa acacaccatt
      721 tttgcatggt caacattact tgcgacaatt gtgtttatta ttattggttt agcattattt
      781 gaaaattata cttcgcttcc ttattatttt ggtattatta ttgcggtaat tccagttgtg
      841 attagcgaca ttttgtggtt atatattagt agaaagaaaa gcgaggcaaa aaatgaaggg
      901 actatttaaa agattaggtc aaggaattct ttttatccta gcgctaccat ttgggatagc
      961 gatctttatc cttttttcaa tttacgccat tattactttt ttctatactt tcttttctgc
     1021 tattccagca ttctttaaag gtgaaaatgt cttcggtcca accgaattag atattgccgc
     1081 tagtacacgc ttagccgaac aaaaactagc agcgcaacaa ccaccaccgc caccagtcgt
     1141 gcaaccttca acaactatta acttatttac agtacaacaa cccggcaccg aacaagtgcc
     1201 accaccacaa gtcacttata cccaaagtga tggtgttact tatcgccgtg ttactgaaca
     1261 agaagttgca gcacttcctg aagaaactat tattgataat agcgaggagc aaaaatgatt
     1321 ttaaatccgt atatattaat taagcttagt gaaagtgaaa aaaggttttt aatcgttctc
     1381 tttttcctaa ttgtcgttgt gctttttgta tgcggactat tttatgaagg tattacgcgc
     1441 tttatggcac gccaaggtga agatattggc agactcatgg caacacaggt ggaatcgggt
     1501 tatatatctt cacttaaaca atttaaaaga aactcttacc gtaaatcacg cttagaattt
     1561 tatcgtcaat ttataaaaac attcattatg atgattatct ggtttgcgat ttatggtact
     1621 gttagtatct tttaccgtca ctggttagat ttatttgatt acactagtga aggttttact
     1681 accctcttat ggaaatttga ttacgctaat acgccacgca ctactttctt tggcatgagc
     1741 attattagtg actggccacc tcttcttcat aaaccattct tcagcgctaa agcaattcca
     1801 agctaccttt tagcggtgtt cggtattgtg attatcattt tatttgttgg ccaagtactt
     1861 tcctacttag cgcgtacgat ctttattatt caacatagta aaaagatgtt tactcgcgat
     1921 ttacgtgatt ataaattaag tgatttaagt gccgctcctg ctgagttaca agaaagcggt
     1981 gtgaatattc ctgaagttaa acctaaagag cccttaccac cgactaacgg gtaagcatta
     2041 caccaatttg aattccaata ataattatta aaataataat aatgatacct aaaacgaggt
     2101 tccgtctttt ataaccacgg attgctattt cttcttttgt taaggggata tcttgtccgt
     2161 atttatttgt attcactttg cacctctaat atttaattga gccaaatacc cgagcgtaag
     2221 gttgaacatc acgaatattt tgcactccag ttaagtacat caataagcgg tcaaacccaa
     2281 taccgtatcc agaatggggg acaccaccat atttacgtaa gtctaaatac cattctaagt
     2341 cttcaaggtt accgccttca atcatctttg cttttaaaac atcgtatcta tcttctcttt
     2401 gtgaaccacc aactagttca ccaacacctg gcactaataa gtcacacgct gcgactgttt
     2461 taccatcatc atttaggcgc atataaaatg ctttaatgtc tttaggataa tcagtaacaa
     2521 acactggacc attcgtataa acttcacaaa gataacgttc atgttcactt gctaaatcca
     2581 taccccagaa aatattgtca aattggaatt taacgccttt ttgctgggct tcttttaata
     2641 tttcaacagc tttagtgtat gtaatacgta atactttact tttcactaat gcttctaaac
     2701 ggtcttttaa tgtcttatca taaaatttcg taaagaaatc aatttcatcg gaacattttt
     2761 ctaatacata agcaacagaa tacttaataa tttcttcaat tgaatccata ttaccattta
     2821 aatcggtgaa cgctagttcg ggttcaagca tccaaaactc cgctgcgtgc cgggtagtat
     2881 tactttcttc agcacggaat gttggtccaa aggtataaac tttaccaaat gttaacgcaa
     2941 atgattcaac atggagttgg cctgagacag taagtgaagc ttctttacca aaaaagaagt
     3001 ttggatcttt atgatcagta acaacattaa agacattacc cgctccttca gcgtcgtttc
     3061 cggtaataat tggtgtatga acatagacaa attcatggtc gtataagtat ttatgaagcg
     3121 catatgatag ttcactccgg agtcggaata aagcagtgtg tgtattggtc cgcggtctta
     3181 agtgcgcaat ctctcttaaa aattcaggag tatgccgttt cttttgtaac ggataatctt
     3241 ctgcaacgtc accaacaaga acaactttat cagcgtgtac ttcaaagggt tgcttcattt
     3301 ctggagttaa cacaactttt ccgtaaactt caagcgccgc tccagtgcga tatttatcaa
     3361 cttcagcaaa gttatcaatc ttattttttt cataaactat ttgtacaccc ttaaaggcac
     3421 taccatcatt aaattcaata aaaccaatcg tgccattatt acgatttgtt ttgacccatc
     3481 cttgtaattt aacaggaatc gctttaagtt cgcctttttc ataggcggta tatagtgctt
     3541 ttacatttaa catattgtta cctctttgac ttctttatta attatatctt aaaactctct
     3601 aaaatcttca atttcccaat taggatcaac acctaaatca taatccgcaa aaactttttc
     3661 atcatataag tatgatgcta gcttagcaat catcgctgct tggtcagtac aacaccatag
     3721 tggcggtatt gcgggatcaa ctccaatttt cgggaatttt gctagtactt cgctccgtaa
     3781 atatgagttt gcgctcacgc caccagcaat tacaacttgt ttgactttat attcttgtgc
     3841 tgcttttaat ccttttttaa taatggcatc aattgcgact ttttgaaatg atgcacataa
     3901 gttcggcaca tttatttctt cgcctctttg tttagtatta ttaactaaat taattacatg
     3961 tgtctttaac ccggaaaaag aaaagtcgta accagttaag gcaggacttg gtaatttgta
     4021 agttgcttca ccttcttttg ctaaacggtc aattgggata ccgcccggat aacctaaatt
     4081 taagacacgc gctattttgt catagcactc tccaatcgcg tcgtcacgcg tttccccaat
     4141 tgttgtaaag tctaaatgtt ttttcatcac aacaaattca gtgttaccgc cacttacaac
     4201 aatagcaagc agtggaaaca caagcggttt aacaaattca ctagcgtaaa tatgcgctgc
     4261 taagtggtga acagggatca gcggtttgtt gtaaaggagc gctaatgttt tagcagcttg
     4321 taacccaacg tggagcgccc caataagtcc aggtccgcga gtcaccgcaa aagcatcaac
     4381 ttcttcaagc ttaatattcg catcttttag ggcagtatct aatgtataaa caatgttttc
     4441 ggtgtgaagt cttgaggcta attcaggcat aactccgcca tacttttgat gcatcgaaat
     4501 ttgtgtacta acagcgttac ttaaaagttt gtcaccacgc ataatagcaa cagcggtttc
     4561 atcacaacta gattcaattg ctaatattat tcgttctttc attcttttag tacctttatc
     4621 atataataag catcttcacc gtcttcataa tacttccgtt tcttagtcac aacattaaat
     4681 ccgtatttta aataaagatg cattgcttta tcgttactcg ttcttacttc taatgtaata
     4741 gctcttactt cttcaagcat tgacactctt tcaatgagat gcgccattag taaacttcct
     4801 agtcccttgc cttgcgccgc gggatgcaca gcaaatttaa caatggtggc actatcaaac
     4861 gtaatccaaa aatgggagaa tccaagaatc acatcagcgt aagttaagac aaatagatat
     4921 gcaaattcat tttttaattc ttgggcataa gtatatttag gccacggatc agggagtgcc
     4981 gcaaacgcta gttcatcaac agcgtcaaca tcgtctatag tcattggacg aataataagt
     5041 ttcttttctt ttgtttccat cttattatcc ctttaaataa agcggcttaa acgctgctat
     5101 gtccttaact ctattttctt cactaatgct tttaagcata ttttcaaaac gattaaactt
     5161 tgcgggttca attcctaaat gttttgtttc gcctgaaatt aagtaacctt ccttttccgc
     5221 tagtgctaat atttcgctgt tttgcattat tttgtccggc aaaatggcat tattttgcgc
     5281 gtaaacccca gcaaaagaac gctctcttct tgcatcaagt acacaaatgg tcggttttgt
     5341 ttcatggcga taaacactta aactagaaac agcataaagt gggacctctt tagcgtacgc
     5401 ccatactttg gcaattgtta cagcaattct taccccagta aaagagcctg gaccacttgc
     5461 gaccacgatt cttttaatgt catcacttgt taatttttcc tgcgctaata gtttattaat
     5521 tcttccaacc attatttcac tttgggtttg aaaagccttt tcttcaacaa aacttaaaac
     5581 tttgtcgccc tttgcaagcg caacacttaa atgatgactc gaactatcta aaagtaatgt
     5641 aatcatttga actcctcagc gataactttt accacgtccg cataatgacc ttcccctttt
     5701 aatgtaatgt ttcttgaagt taaatcgcga tgcgtaatat taatttcaag tgccttttcg
     5761 ggtaaaagat gcttaataaa tgccggccac tcaataacag taatacctgc ttcaccaata
     5821 tattcatcta gtccaatatc ttttttatca tcttctaaac gataagcatc aatatgaaat
     5881 aatggtttat caccttttaa ataaatctta agaatattaa aggttggact attaactttt
     5941 tctgtaatgt ttaaaccgct cgctattcct tttgtaatcg ttgttttacc agcccctaag
     6001 tcaccagtaa gcagcacgac atcacctaaa ttaaataatg ccgctagtct tttacctagc
     6061 gctattgttt gttctctact aatacttata aattcatgtt tcatagacgc caaaattata
     6121 ccatgattag ttaggtttca atttataatt aaaatatatt attttttaat ctttgtttct
     6181 tcaacaaatc gattataaag tttccgaact tcatcatcat caaacggaaa cgttttttca
     6241 tttatcatcg ctacgacttg cgtttgttta tattcaacaa ttgcggataa ttctttggaa
     6301 tattgatagt tttcaatatt gcgcaaaaat tcatttttat caaggtattt aatcgtacca
     6361 tcactaaatt tcttcacatc taaatcgtaa tcaatatact tcgccatccc gtaatcaatc
     6421 agtgtcggtg aagctatatt tgtgtaataa acaacacctt cagttttaag catagcaatc
     6481 acattaaacc agtcgttttt atgaaaaaag gtaaccgctg gttcttttgt aaaccacttg
     6541 cgaccgttac cttcaattac ttgtgtacgg cgtgaagcga cgattaacca ttcatcgtta
     6601 tcttctaaaa cataagactt acgccaaaaa cgatgtaaat taccatcgtg tttatagctt
     6661 tggattttaa tccatttacc tttgatactc ataactaact caactctcga ttgttagtta
     6721 gattttaaca taaaaactga tgtttttact atagtaaagc gataaatttg tttaaagctg
     6781 ttaaatgctc tttatatttc ttaattgcgt gcactggcac attctcttta tatggcacaa
     6841 ccattaaagc atcactataa gtaagtgtaa ctgcaaattt atttttatat aatgcgacac
     6901 ttacatcggc gagttcgtta tcaggacgaa cttgtaacag tgcttcaagt gcttttgtag
     6961 aaatagcttt tttaagttct ttattcgttg catataattt gtattctggt ttatccacga
     7021 ctagttctaa gtcttcaaga tcatctggac cagtgccacc atcatatttt tgttcacttg
     7081 gtttaatata aagcacaagt cgtccatcaa tttctttttc agtcgttcct gtcactaatt
     7141 ttcctaagaa aacagcacgt gcttgcttac catcgcgtct ataagcggcg taatcagcaa
     7201 ttttgacatg ccagcgtcct aagtcataag taactaagtt acgagaatta gaattaataa
     7261 tgtctttaag taatcgagcg tcagtaaacg ctgctttatc taattgacca gcaaaatcaa
     7321 agacataatt attaactttt gattcactaa gcgcaagttc attcacaaag ccattgtaat
     7381 cagccatata ttttcgcaca ttgtcagtaa gtttgttacg cgcccagcgt gaatacacta
     7441 aagaagcaac aagtactaaa ataattggaa caataccagc ccactgatta attggtagta
     7501 accacacata agaaacaact atcacagcaa taacaattgc agttgacaca taactaaaaa
     7561 tacggaactt tcgatacatt ttcgaaaaag tattccgcat tgtttctaat gctggtaatt
     7621 ctaattgttc gttagtagcg tcatcactaa tttcttcatt aacaaatgct tgtggagttt
     7681 tagtttctgc aggagtttcc tccactacct ccaccatctt ttctttttct gtcatataat
     7741 tctctattct atgtcacgta ataagcgcgc agcaatacgg tttcctttcg cggcagcacg
     7801 ctctaaatat tcacgggctt tcgttagatc tcgtagtagt cctcggcctg ataaatgagc
     7861 tttaccaact tccacttcag ccttaacata accgagattt gcagactttt cgtagtaact
     7921 aaatgctttt gtcacatctt ttgttacacc catcccgtgt tcatggaagt aacctagttt
     7981 gtagaaagct ttggcaaatt tttgatcagc cgctctttga taccattcaa gcgctaaagc
     8041 gtaatcttgc ggaataacag tcccgcgctc gtaagctatc cccatattat attgagcagc
     8101 gtcgtgacct ttaactgctc cgctatggaa gtaagcaaat cctttttgtt tatcttcttc
     8161 aacacctttg ccataaaaat aacaataccc aatcacagtg tccgctggcg catagccaag
     8221 ggcttctgat tggcgcgcaa gttcaatgcc gcgcttattg tcttctgcaa taccttcacc
     8281 ttgcactaaa cgcctagcaa gttcatgcat tgcagcggga tcattacttt taataagccg
     8341 ttcaagttct tgatttgtct tatttgtata atttaaaatc atcataactt cttccccctt
     8401 ttagtataac atactaaacg cggttgatgt tattgttgtt tgctttcttc tacttcgttt
     8461 tgtaggaatg gttgtggtct aaatcttagt aatgtcggga aaagcatcat taaaagaacg
     8521 ccacaaataa ttccagacac accaattgca ggtaaattat ataagagtga accgacaaat
     8581 ggtgtttcat agaccactac accactgata gtagaaaaga aaattcgccc acttacaccc
     8641 gcaggtactg caagagcaag atataaataa ttaattattt cgtgtttatg tttgccatac
     8701 actaaagctt ggaataaaga aattatagaa agaaacccat acgctaataa gtaatctaaa
     8761 ggataagtaa ttaaaccgta tccatcccat aacatcgtga taacaccata aattccgcct
     8821 atcccaataa aggcgtcaac aagggggaaa cgcaaagcaa taatcatcag tggtaacatc
     8881 gtaatactaa atgattgtgc ttcaccaagc cgaaatttta aaaattctaa atcaaacact
     8941 aatgctaaag caataaatat tgccatttcc gtaataccac gcactgttaa aatacgacca
     9001 aaaaatctgt ttggttttaa tgttcttctt ttttttgttg tttctttcat aattagtctc
     9061 ctttaaacaa aaagtctcca cgctgacgtg aagactctta tgttacatcc ctacgccagc
     9121 att
//
LOCUS       DAOSTA010000036         8883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_7501_length_8883_cov_8.906208, whole genome shotgun sequence.
ACCESSION   DAOSTA010000036 DAOSTA010000000
VERSION     DAOSTA010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8883
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7501_length_8883_cov_8.906208"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..445
                     /locus_tag="PLU08_04000"
     CDS             113..445
                     /locus_tag="PLU08_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70885.1"
                     /translation="MEQNFRARREKIFGRFKKEQVLRWMMSFTVTTSVAVTAVVGAST
                     AEPEVSFTSVEVLGNTIYYETLVNDPDLTLESESLVLDVKSGLETFSFPLSLGITTGN
                     FNIRYFNI"
     gene            539..1465
                     /locus_tag="PLU08_04005"
     CDS             539..1465
                     /locus_tag="PLU08_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70886.1"
                     /translation="MATHYTEERQAFTFTFDIFIFNPDLELLDITLFPSFAFSEPGEE
                     GVVTEIIELPRINLEEGTNVCTLESIPTHASFVSLKIAGTKNDEEVILLDESIPLPST
                     FSSYIHAVGFSATSLSLVAEVFAPLLFEGTATLNLYEEETLIAAREFNVPYVDAEGEY
                     IPPIFDFENLSVNTMYTAQIEIPYMNHIRKQNEVYKSELQNVYTAPYYSYNAEHILYE
                     NEMVYITLDVVDEGSIISGAYAECFARDEYGELVPMYINSFENVSATAGESNQYECMV
                     FYTEQNIVEIRVYINKSYNYQYENELIYIVTI"
     gene            1480..2445
                     /locus_tag="PLU08_04010"
     CDS             1480..2445
                     /locus_tag="PLU08_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOQ70887.1"
                     /translation="MSKKFKFNKGLIPKLIVIGIFLALVIIAIISDKVLTGTAFGDFV
                     NNTIGKFFNIIDILKNHWITIAESIVIIVFFWLLNKLFEYILLPLGKKRNKHSTWWVL
                     FRSIFKYLSSAVAIFLVLSAWGVDTPTLLIGAGIVGLAISFGAQSLIEDVIAGLFIIF
                     EKQFKIGDVIQVGETRGYVIDIGIRTTSIEDVYGDILIVNNSDLRMILNTSANLSPAI
                     SEIYISYDQPLENVENIVKKALPLLKERIPDIIEGPTYRGVQKLDSNGVLIRVYAKVE
                     DLKKYQVVRDMNRELKIVFEKNNIKFGFPTVNVNEREEEVKKAKD"
     gene            2510..3286
                     /locus_tag="PLU08_04015"
     CDS             2510..3286
                     /locus_tag="PLU08_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70888.1"
                     /translation="MENNTSQKISFKFDLYTIIIGALLGLSFVMSLVHNIIRQMGFIA
                     FEFWLLRYAFLALLFCIFLVARALKHKELMLITTTLIVAHYLIFPVGTVSFNGFLDFE
                     IQAQFIVALVLIALILLIVASFIKVDIYEKVETFYKYVVAVLFALIGFVFLLDTFING
                     FRDYGDFRWTRAFFALSGVFYYAALVGLFALPILDTGILTPKKKETVQEVEVEVKSEE
                     EKPKEAEKVEAAPKKTEVKEPKVKVEKETEKAEPEAEPKE"
     gene            3491..4612
                     /locus_tag="PLU08_04020"
     CDS             3491..4612
                     /locus_tag="PLU08_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HOQ70889.1"
                     /translation="MNQDFIYGADISSLLAVEELGGTFTSDSGEKQELFEILKSNGFN
                     LARIRLFVDPKSNKGVSYGAGHNDLETALTLAKRAKDHDFKVLLNLHYSDFWADENHQ
                     TLPKAWAKLDFADLEYQVYAYTKEVVTTFKEENITLDYIQVGNEVNKGILWPHGKLFR
                     RKKATQTEYDALAILLNAGIKAIREVTPNVKIMIHLGQGSNPEMYADFLHFMEPRLFK
                     FDAIGLSYYPYIHRQITELQATLLFIKTYYPYERFIVETSYPFENGDETSIVNKDSFP
                     PKRGFLAYTPLGQERVLETILDAAVEEQITGLCYFEPGWINVNGLTVATIEGIKYLKE
                     KNIKAGSTLFNEALFSRSGRPNPALSVKNNMDAFKKSDN"
     gene            4750..5646
                     /locus_tag="PLU08_04025"
     CDS             4750..5646
                     /locus_tag="PLU08_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ70890.1"
                     /translation="MDIILQTNNLSKRFKNKLALKNVNMTIYRGDIYGFVGENGAGKS
                     TLIRLVTNILRPSTGSYSLNLPKRLGVVGAIVESPAFHPGLNALQNLRYQSDLLGYKK
                     TDEELLVLLDKVGLEEQRNSKKVVKNFSLGMKQRLSIAVTLLSNPEFILLDEPMNGLD
                     PAGIKEMRDLILELNRKGTTFLISSHILTELDKVATRYGFISHGELVEEISVRDLHKK
                     AKSYTEVKLGSEVNAKVRELLSGLNFKEASATTLHFATVAEAQNALKLLVANDISVTS
                     FEEIHETIEDYYLQKINKGVGA"
     gene            5649..6419
                     /locus_tag="PLU08_04030"
     CDS             5649..6419
                     /locus_tag="PLU08_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70891.1"
                     /translation="MKKLLIADLRRIFKGKGIYVLMILGFIFPAISALIVQLSGLAND
                     VAELGDLAAEVFNPVALYAGSFNPTQNIGLILLIVVGVLISGEFTNNTIRNKIVAGHS
                     KTSLFLSSLVTVLTYVFVVVLVNSSFTYLFNSISFGLQPFSDYFKRGLVAYSSIFVIY
                     TIMTLLTYQFKNLAGPLGLVLGIYFVLMMISTIIFSFNGGTGFKAKVLFYIFPFLYLS
                     GIATPIKLEWLWATLTNVGYLALLTTLGILLANRTDYK"
     gene            complement(6420..7376)
                     /locus_tag="PLU08_04035"
     CDS             complement(6420..7376)
                     /locus_tag="PLU08_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOQ70892.1"
                     /translation="MLTRVIGLDIGAATMRAAVVDREGTIYYSKEVATPERSELRFVR
                     EINDLLENVLFHAPEAIAIGVGVPGIVSPGTTLIHLLPNLGYENIDFKRILRKEIKQP
                     VYLLNDANAACYAEALFGAGADKRIVQYITLSTGVGGGLVINKEIYTGARGFALEIGN
                     MIIDTETPRPNDKMNRNSFEGSCSGKSLYNQAKRFGTNIHTVASVFNESRFSPIRNVW
                     VDNLGKGIANIYSLYDPDVVILGGAVMQSADKFFDDILPAVEKYTLPQLHPYINLKRS
                     HFGETGGMIGAALYALHEHEKIINKAKSDEVKIDTEVKAETK"
     gene            8127..8294
                     /locus_tag="PLU08_04040"
     CDS             8127..8294
                     /locus_tag="PLU08_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70893.1"
                     /translation="MSVVLLALLKNFAVMPNVDEPAKALEAYKLTGGAVVALVLAGLG
                     GVAGVAADLLK"
     gene            complement(8381..>8883)
                     /locus_tag="PLU08_04045"
     CDS             complement(8381..>8883)
                     /locus_tag="PLU08_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOQ70894.1"
                     /translation="GYKLFDLGGKNFAYIGVTRKLNINNERLNGFLKALKNNDVIVPE
                     ANIILNEKQQTTRQLMDTLDVQKKKIDSIFCFNDQIAYEVIGYLSEKGIKVPEDINII
                     GFDNLQSAIIYPIKLTTIDADKPRAAHLALVSLFSKIKDSEDTEVTGKIVDVTLVEGN
                     TTKMRK"
BASE COUNT         2815 a         1383 c         1685 g         3000 t
ORIGIN      
        1 aaacattata atgaagacaa gaaggatgta aatttaacct tttcctttaa cgaagcatta
       61 atatttgctt gtaattttat ttattccagt agtaaaatgt ttttaagcat atatggaaca
      121 aaatttccgc gcccgtcgcg aaaagatttt tggacgattt aaaaaagaac aagttcttcg
      181 ctggatgatg tcttttacag tgacgactag tgtcgcggtt actgcggtag tcggtgccag
      241 taccgctgaa ccagaagtat cttttactag tgttgaagtg cttggtaata cgatatatta
      301 cgaaacactc gttaatgatc ccgatttgac acttgaaagt gagtcacttg tacttgatgt
      361 aaagtctggg ttagaaactt tctcgttccc gctttcttta ggaataacaa cgggcaactt
      421 taatatccgt tattttaata tttaatatga actagccatt aaaggtccac aaggctacgg
      481 taaaaagact tttgcaactt ataacattat tagtgttagt gaaccggcag cgcgcatcat
      541 ggccactcat tacactgaag agcgtcaagc ctttactttt acctttgata tatttatttt
      601 taatcctgat cttgaattat tggatataac gctattcccg tcttttgctt ttagtgaacc
      661 aggtgaagaa ggagtagtga cagagattat tgaattacca cggattaatt tagaagaagg
      721 cacgaatgtt tgcacacttg aatcaatacc gacgcacgct agttttgtta gtcttaaaat
      781 agcgggcact aaaaatgacg aagaagttat tttattagat gaaagtatac ctttaccttc
      841 aacattttct tcttatattc acgccgtggg gtttagtgcg acttcacttt cactcgtcgc
      901 tgaagttttt gcgccactac tttttgaagg aacagcaaca ctaaatcttt atgaagaaga
      961 aacacttatt gcagcacgcg agtttaatgt accttatgtt gatgctgaag gagaatatat
     1021 tccaccgatc tttgattttg aaaatttaag cgtcaataca atgtatacag cgcagattga
     1081 aattccgtat atgaaccata ttaggaaaca aaatgaagta tataagagtg aattacagaa
     1141 tgtgtataca gcgccgtatt actcttataa cgcagagcat attttgtacg aaaatgaaat
     1201 ggtatatatc acacttgatg ttgttgatga aggaagcatc attagcggcg cttatgctga
     1261 atgctttgca cgcgacgaat acggcgaatt agtgccaatg tacattaata gtttcgagaa
     1321 cgtgtcagct actgcgggtg agagtaatca atacgaatgt atggtatttt acactgaaca
     1381 aaatattgtt gaaattagag tgtatataaa taagtcatat aactatcaat atgaaaacga
     1441 attaatttat atagttacca tttaaaggag ggaacgatta tgagtaagaa gtttaaattt
     1501 aataaaggac taatcccgaa gctaattgtt attggcatct ttttagcgct agtgattatt
     1561 gcaattatta gtgataaggt tctaacaggt acagcgtttg gtgactttgt taataacacg
     1621 attgggaagt tttttaacat tattgacatc ttgaagaatc actggattac cattgcggaa
     1681 agtattgtga ttattgtgtt tttctggctc ttaaataaac tctttgagta tattttattg
     1741 ccactaggaa agaaacgtaa taaacactca acatggtggg tattatttcg cagtatcttt
     1801 aagtatctta gtagtgcagt agcgattttc cttgtcttaa gtgcgtgggg tgttgatacc
     1861 ccaacactct taatcggggc cggaattgtc gggttagcga ttagttttgg tgcgcagagc
     1921 ttaatcgaag acgtgattgc tgggttattt atcatctttg aaaaacagtt taagatcggt
     1981 gacgtaattc aggttggaga aacaagagga tacgttattg atatcggaat tagaacaact
     2041 tcaattgaag atgtctatgg tgatatttta attgtgaata acagtgactt acggatgatc
     2101 ttaaatacaa gcgcaaactt atcacccgca attagtgaaa tttatataag ttacgatcaa
     2161 ccactggaga atgtggaaaa tattgttaaa aaagcattgc ctttactaaa agaaagaatc
     2221 cccgatatta ttgaaggacc gacatatcgc ggtgtacaaa agcttgatag taatggggta
     2281 ttaattagag tatacgcaaa ggttgaagat ttaaagaagt atcaagttgt gcgtgatatg
     2341 aatcgtgaat taaaaattgt gttcgagaaa aataatatta aatttgggtt ccccactgtt
     2401 aatgttaatg aacgcgaaga agaagtaaaa aaggcaaaag actaaaaaag tggtagttat
     2461 ttagtttttt ctaaactata atgaatataa agaaaagggg aaaaaatcta tggaaaataa
     2521 tacttcacaa aagatttcat ttaaatttga cttatatacg attattattg gtgcattact
     2581 tggcttatca tttgtcatgt cacttgtgca taatatcata agacaaatgg gctttattgc
     2641 ttttgaattt tggctactgc gttatgcttt tttagcatta ctattttgta tctttttagt
     2701 ggcaagagcg cttaaacaca aagagttaat gcttattaca acaactttaa ttgtggcaca
     2761 ttatttaatc tttccggttg ggaccgtttc ttttaatggt ttccttgact ttgaaatcca
     2821 agcacaattt attgtagcgc ttgtcctaat tgctttaatt cttttaattg tcgcttcctt
     2881 tattaaagtt gatatttatg aaaaagttga aacattttat aaatatgtgg tcgcggtatt
     2941 attcgcctta attggctttg tctttttact tgataccttt attaatggct ttagagatta
     3001 tggtgacttt cgctggacaa gagcgttctt tgctttaagc ggtgtctttt attacgctgc
     3061 tttagtggga ttattcgcgc ttccaattct tgatactggt attttaacgc caaagaaaaa
     3121 agaaacagtg caagaggttg aagtagaagt taaaagtgaa gaagaaaaac caaaagaagc
     3181 agaaaaagta gaagctgctc caaaaaagac agaagtaaaa gaaccgaaag taaaggttga
     3241 aaaagaaaca gaaaaagcag aacctgaagc agaaccaaaa gagtaatagt tttactttta
     3301 ttaaatttat atgctactat aaaaatacac cttgggcggc actaggcagg aaccaaataa
     3361 tagctcaagg tgggcgacac tacttgttag tgtcgttttt tttattacta tgttgtgact
     3421 tttaatgaaa gttaagcatt agtcatttat aattatagtg ttgtattgct tagttttagg
     3481 agggaagata atgaatcaag atttcattta tggtgcggat atctcttcac ttttagcagt
     3541 cgaagagtta ggtggtactt ttacgagtga tagtggtgaa aagcaagaac tttttgaaat
     3601 attaaagagt aacggcttta atttagcgcg gattcgctta tttgtcgatc ctaaatcaaa
     3661 taaaggtgtt tcttatggtg cgggtcataa tgatttagag acggcattaa cacttgcaaa
     3721 aagagcgaag gaccatgatt ttaaagtatt acttaatctt cattatagtg atttttgggc
     3781 agacgaaaat catcaaacat tacccaaagc atgggctaaa ctagactttg cggatcttga
     3841 atatcaagtc tatgcctaca ctaaagaagt tgttactact tttaaagaag aaaatataac
     3901 acttgattat attcaagttg gtaacgaagt taataaagga atcttatggc cgcatggtaa
     3961 gcttttccgt cgtaaaaagg caacacaaac agaatatgac gctttagcga ttttacttaa
     4021 tgctgggatt aaagcaattc gtgaagtaac accgaatgtg aaaataatga ttcatttagg
     4081 gcaaggttct aatccagaga tgtacgcaga tttcttacat tttatggaac caaggctttt
     4141 taaatttgat gctattggat tatcttatta tccgtatatt caccgccaaa ttactgaatt
     4201 acaagcgaca cttttattta ttaaaactta ttatccttat gaacgcttta tcgttgaaac
     4261 tagttatcct tttgaaaatg gtgatgagac aagtattgta aataaagata gtttcccacc
     4321 gaaaagaggt ttcttagcct acacaccgtt aggacaagag cgtgtccttg aaacaatttt
     4381 agatgcagcg gttgaggagc aaattacagg gttatgttat tttgaaccag gatggattaa
     4441 tgttaatggt ttaactgtgg caacaataga aggtattaaa tacttaaaag aaaaaaatat
     4501 caaggccggc agtacattat ttaatgaagc tttgttttca agaagcggcc gacctaatcc
     4561 agcacttagc gttaaaaata atatggatgc ttttaaaaag agtgataatt aaaagggaga
     4621 gtcctcgcaa gtagcgaggg cttttcattt gtaaagaaaa gtttactaac acgcttacga
     4681 gtttttcgtt tagtgttaag tagtgtataa ttacaaagta attaaaagtg aaagtagaga
     4741 agtgcattta tggatattat cttacaaacg aataatctct ccaaacggtt taaaaataaa
     4801 ctagcgctaa aaaatgtaaa tatgacgata tatcgcggtg atatttacgg gtttgttggt
     4861 gaaaacggcg ctggtaaatc aacactgatt cgcctagtga cgaatatttt acggccttcg
     4921 actgggagtt attcattaaa cttaccaaaa agacttggcg tagtgggagc gattgtcgaa
     4981 agtcctgctt tccatcccgg cttaaacgct ttacaaaatt tacgctatca aagtgattta
     5041 ttaggttata aaaagactga tgaagagtta ttagtgttac tcgataaagt tgggttagaa
     5101 gaacaacgca atagtaaaaa agtcgttaaa aactttagtt tagggatgaa acaacggtta
     5161 agtatcgccg tgaccctttt aagtaatcct gaatttattt tgcttgatga accaatgaac
     5221 gggcttgacc ccgcgggcat taaagagatg cgtgacttaa ttttagaatt aaatcgaaaa
     5281 ggtacgacat ttttaatttc tagtcacatt ttaactgagt tagataaagt agcgacgcgt
     5341 tacgggttta ttagtcacgg tgaattagtc gaagaaattt ccgtgcgtga cttgcataaa
     5401 aaagctaaga gttataccga agttaaatta ggtagtgagg taaacgctaa agtgcgtgaa
     5461 ttacttagcg gtttaaattt taaagaagct agcgcgacaa ctttacactt tgcgactgtt
     5521 gcagaagcac aaaacgcttt gaaactttta gtcgctaacg atattagtgt tacttctttt
     5581 gaagaaatcc atgaaacaat tgaagattat tacttacaaa aaattaataa gggagttggt
     5641 gcctaattat gaaaaaatta ttaattgctg atttacggcg gatcttcaaa ggtaaaggta
     5701 tttatgtctt aatgatatta ggttttattt ttccagcgat tagtgcgctt atcgttcagc
     5761 tttcaggtct cgctaatgat gtggcagaac ttggtgactt agcagcggaa gtatttaatc
     5821 ctgttgcttt atacgctggt agttttaatc caacacaaaa cattggttta attttactca
     5881 ttgttgttgg tgttttaata tctggagaat ttacaaataa tacaattcgg aacaaaatcg
     5941 ttgcaggaca ctcgaaaaca tcgcttttct taagttcttt agttactgtt ttaacatatg
     6001 tttttgttgt cgtattagtt aatagttcct ttacttacct ttttaatagc ataagctttg
     6061 gcttacaacc ttttagtgac tactttaagc gcggattagt tgcgtatagt agtatctttg
     6121 tcatctatac gattatgaca ctattaactt atcaatttaa aaacctagcg ggaccactcg
     6181 ggcttgtgct aggtatttac tttgtgttaa tgatgattag taccatcatc ttttctttta
     6241 atggggggac tggttttaaa gccaaagttt tattttatat attccccttt ttatatttat
     6301 ctggaattgc tacaccaata aagcttgaat ggttatgggc aacattgacg aatgttgggt
     6361 atttagcctt gcttacaaca cttggtattt tattagcgaa tcgcactgat tataaataat
     6421 tatttagttt ctgcttttac ttcagtgtct atttttactt cgtcactttt tgctttgtta
     6481 attatttttt catgttcgtg aagagcataa agcgcggcac caatcatccc gcctgtttca
     6541 ccaaagtgtg accgttttaa attaatatac ggatgaagtt gcggtagtgt atacttttcg
     6601 accgctggta aaatatcatc aaagaattta tccgcacttt gcatcacagc accaccaaga
     6661 attacaacat caggatcata gagtgaataa atattagcga tacctttccc taagttatca
     6721 acccacacat ttctaatcgg ggagaaacgg ctttcgttaa acacacttgc gacggtatga
     6781 atattcgtgc caaagcgttt tgcttggtta tataaacttt tacccgaaca actcccttca
     6841 aaactattac gattcatttt gtcattaggg cgcggtgttt ctgtatcaat aatcatattg
     6901 ccgatttcta aagcaaagcc acgtgctcct gtataaattt ctttgttaat aacaagtccg
     6961 ccaccaacac cggtcgataa agtaatatat tggacgatgc gtttatcagc accggcacca
     7021 aaaagtgctt cagcataaca cgctgcgtta gcgtcgttaa gtaaatagac aggttgcttt
     7081 atttctttac ggagtattcg tttaaagtca atattttcgt aacctaagtt tggtaagaga
     7141 tgaattaatg tagtgccggg gctaacaata ccaggaacac caacaccgat ggcgattgct
     7201 tcaggggcat gaaataaaac attttctaat aaatcattta tttctcgcac aaagcgtaat
     7261 tcactgcgtt ctggtgttgc aacttcctta ctataataaa ttgttccctc acggtccaca
     7321 accgcggcac gcatagtagc ggcaccgata tctaatccaa taactcgtgt taacatatta
     7381 atttgtcctt tctaaattca ttattactac tattaattca acactaaaga aatgtaagag
     7441 tagtaactat gaatttcttt ccttatgaat aattcatcgt aataatttta tcataattgt
     7501 gccttagtga tggtaaaggg ttatgcgaag cattcataat ctttacattt agtcttgaat
     7561 tactaatatt agcactaaat taaaagcaag ataaagtcct ttattttaag gtgaaaagtg
     7621 tgaacaaaac ggggctttgt tcattactaa ttgtaaaata ttttttaata tcacttttcc
     7681 aaagagaagt acttaataac ttatgcccat tatacgctaa ttattcggtt ttaagtgctt
     7741 atttactaaa tgaacaaccg tttattattt tgtgattttg gaaagttaat agttgcctaa
     7801 ctttatattt tatgcaaaag aaaaaattat taaatgtagt cgcagctggt ttatttttag
     7861 ttgccctcat ctgatggttt gccgttcctg cgattgctag aactcaacca gaagtaagtt
     7921 acaccttcct tgatttagcc tttggaagaa ctgaaacgct ccttggagta gaagttatag
     7981 aagttattat tcttaagttt agtttcttaa acttcttagt cttattactt ctccttgctg
     8041 gggttggact tagtgttggc cgtttagtcg ttaaaggttt agaaaaaaat aaacttcttg
     8101 gctttgtcat tttagcgctt ggcttagtga gtgttgtatt actagcactt cttaaaaact
     8161 ttgcggttat gccaaatgtt gatgaaccag caaaagcact agaagcttat aaacttactg
     8221 gtggtgccgt cgtcgcttta gtccttgctg gacttggtgg tgttgctggt gttgccgctg
     8281 atcttttaaa ataagattaa tttttaatta aaatgaaaga taacgcttcg tcgcgttatc
     8341 tttttttata tttttattaa ttatttgcgg caaaaaggac ttattttcgc attttagttg
     8401 tgttaccttc aacaagtgta acatcaacta tttttccagt tacttcagta tcttcactat
     8461 ctttaatttt agagaataaa ctcacgagcg ctaaatgggc ggcacgcggt ttatcggcgt
     8521 caattgttgt taatttaatt ggataaataa ttgcgctttg taaattatca aagccgataa
     8581 tattaatatc ttcaggtact tttattcctt tttcacttaa gtagccaatt acttcgtagg
     8641 ctatttggtc attgaaacaa aaaatggaat caatcttttt cttttgcaca tcaagcgtgt
     8701 ccattagttg acgggttgtt tgttgctttt cgttaagaat aatgtttgct tcaggaacga
     8761 taacatcatt atttttaagt gcttttaaga acccgttaag tcgctcatta ttaatattaa
     8821 gcttgcgggt cacaccaatg taggcaaagt ttttaccacc taaatcaaat aatttataac
     8881 ctg
//
LOCUS       DAOSTA010000037         8718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_7684_length_8718_cov_8.303244, whole genome shotgun sequence.
ACCESSION   DAOSTA010000037 DAOSTA010000000
VERSION     DAOSTA010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7684_length_8718_cov_8.303244"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1566
                     /locus_tag="PLU08_04050"
     CDS             61..1566
                     /locus_tag="PLU08_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOQ70895.1"
                     /translation="MKRKYVFLPLLLLGSVSLSSSPSPRVTHFAPHYAAPSTEPSGPQ
                     TLRIYNWEEYISEEDIDDPDCIDVLAAFQEYYLENHGEEVTIEYNTFSTNEDMYNIVK
                     LNGAKYDLIAPSDYMIQRMIREDMLEKYDLDAEGNYKYVPNYNEYASPYLIDLFKEDL
                     GIYEYAPGYMWGTMGFLYNSEFNDTINDDVQSWMMLWDKKYSKKTSIKDSMREGYLMG
                     LFKYYEDELNDLAAQREAGLISDDYYNDEISDLLNDVDPDTIKEVIHVMNELKNNIYG
                     LEVDQGKIDMITGKIAANTAWSGDAVYAIEEDPNDVLRYAIPKEGSNIWFDGWIMPKG
                     ANRVVAQRFVNFVSSPEMAIANMYYIGYTSFIAGDEVLEYVLDNDAVYEAAEAEETVE
                     LDFTYFFSDTIENPSDALITIDASYVGHTLTATYPTEEEIVRSVVMRDFGDSNKLVID
                     MWANFKATDIQNWMIIFSVIIVLAVGSAVTLNQVQKHKAARRKRNLYKDKQ"
     gene            1563..2684
                     /locus_tag="PLU08_04055"
     CDS             1563..2684
                     /locus_tag="PLU08_04055"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOQ70896.1"
                     /translation="MKKRLAVIGGGVSGVTLARLVQGSFNVTIFERESSLLQKLLRTG
                     NGRANIYNRNIHSFAYNDEHFLSEHLPHIVKTLDDLFSAFNIVTYTDTEGRVYPYSRS
                     AKALRALLIKDLKAEVKLEHNVEEIVKVRDKYIVDGEEFDAVALTMGSSAGLFKYPLD
                     NGNAALLSALDLETTALVPTIKTIKVKENLKRIEHERITARVTLMDDENVIASDVGEV
                     LFKKDGLSGIVSFILSSYLEWAVVRYPKRNYYLTLDLMPEFSARYTAEIIKTTDDLDN
                     VFSPNISHYLKGRNEPLLDRMKALTFTPIPKNNPENTSAMHGGIKLSKLNTDFEVRES
                     PNLFALGEVINIDGICGGYNLAFAFYSAKVVSDALLRKL"
     gene            2950..5154
                     /locus_tag="PLU08_04060"
     CDS             2950..5154
                     /locus_tag="PLU08_04060"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOQ70897.1"
                     /translation="MVTHIKKRDGRLQKFNAGKIANAINAAYGNTNDVQSLLYAAEIT
                     SNLNTNYDGTIDIEVVQDEVEKFLMKKDPNIAKKYILYRKDRERIRNAKSTVFNYKKL
                     VEDYLNIADWRVKENSTVNYSLGGLILSNSGAVTANYWLSEVYDEEVANAHRSAAIHI
                     HDLSMLSAYCAGWDIEKIIRDGVGGVEGKISSKPAKHLFTLANQLVNFLGIMQNEWAG
                     AQAFSSFDTYLAPFVKADNLTYAEVKQAIQSFVFGVNTPSRWGSQAPFSNITLDWTVP
                     SDLADRPAIVGGKDMPFKYKDCQKEMDMINKAFLEVMIEGDANGRGFQYPIPTYSITR
                     DFSWEETENNKLLFEMTAKYGTPYFSNYINSDMEPSDVRSMCCRLRLDLRELRKKGGG
                     FFGAGSNTGSVGVVTLNMPRLAYLSRTEVEFFNELDKLMDISARSLKVKRDVVSKLLE
                     DGLFPYTKRYLGNFDNHFSTIGLVGMNEACLNARWLGKDLGDPESQAFTIKVLNHMRE
                     KLSDYQEAYGDLYNLEATPAESTAYRFAKHDKVRYPDIITARLGEDGDPFYTNSSHLP
                     VGYTASISEALDIQDEMQTLYTSGTVFHTFLGQKLPNWQTAARLVRKIAENYRLPYFT
                     MSPTYSICPTHGYLTGEVEICPECKRTTEVYSRITGYYRPLKNWNDGKKAEFENRLEY
                     ADFTIEGGTVHNPKFNAPKVEVKDTADAPIKEQKPTPHKGAPIIFLNKQNND"
     gene            5147..5665
                     /gene="nrdG"
                     /locus_tag="PLU08_04065"
     CDS             5147..5665
                     /gene="nrdG"
                     /locus_tag="PLU08_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019000008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOQ70898.1"
                     /translation="MTKKKIRIAGYVNDSLVDGPGIRFTIFTQGCPYHCLGCHNPDTW
                     DPKGGEEVEISTLIAKWRKNSLLDGITLSGGEPLLQKEALLPLINAARKDGLNITMYT
                     GSTYEELIHRNDPLVNEILSKVDYLIDGPFILHELNLNLLYRGSNNQRIIDLRKTTPN
                     NIVLINTMHDFK"
     gene            complement(5732..6880)
                     /locus_tag="PLU08_04070"
     CDS             complement(5732..6880)
                     /locus_tag="PLU08_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOQ70899.1"
                     /translation="MKNLWTVIKKELKRFFTDKRMLAALILPGVIVYILYSIMGNVMQ
                     STMEGKDHEYVIRVENRPNDPRFLAIETLIADPEVGFKKVTFLDETPEDFEAMIAEKK
                     LDLYVVYNTDFLAKYNANDPNDPPVVTFHYDESVIPSLNAYSTYAGLLNASLQTYVAV
                     PINHSAEGGIDTTIIKGLLPFLLITFIFTGAMAVAPESIAGEKERGTIATLLVTPVKR
                     STLALGKVTALSIVALVSAASSFLGVMLSLPKMMGSELSLATFTAGTYLAIFAVLIVS
                     MLVFIVLNSIISAYAKSIKEASGLAAPLMMVIMAVGVTTMFGSAATNNALYLIPIYNS
                     VNILLSLFGGEFLVVPFILTMLSNLLLTAGGVYLLTVMFSNEKIMFRK"
     gene            complement(6880..7656)
                     /locus_tag="PLU08_04075"
     CDS             complement(6880..7656)
                     /locus_tag="PLU08_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ70900.1"
                     /translation="MQPILEVKNLVKTFKLSKKQQKIEKTNTKVRVAVNDLSFNVYEG
                     EIYGLLGPNGAGKTTTLRIISTLIKKDNGEVKVNGYDISKEQSKVRSQIGFLTSELKL
                     DDFFTPNYLFDYFSHLHGVPEDVIEKRKQELFDKFGISEFKEVKVSDLSSGMKQKVSL
                     VISVVHDPKFIIFDEPTNGLDVITAKTVIDFLVALKEAGKTVILSTHIFSLVERICDR
                     VGVIIDGALIFEEPMSTFSEPNSLEERFFLEYAKVKGDLA"
     gene            7877..8149
                     /locus_tag="PLU08_04080"
     CDS             7877..8149
                     /locus_tag="PLU08_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOQ70901.1"
                     /translation="MSMTNLNIRMDKKVKEEAEAIFQELGLNMTTAVNIFLRTTIREK
                     GIPFHLSLSVSNVTTKAAIEEGRKIALTDNVKGYDTVSDLKAALED"
     gene            8249..8322
                     /locus_tag="PLU08_04085"
     tRNA            8249..8322
                     /locus_tag="PLU08_04085"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8281..8283,aa:Cys,seq:gca)
BASE COUNT         2874 a         1498 c         1729 g         2617 t
ORIGIN      
        1 tagaaaggag agaagttatt cagtcatcaa actaggctta actgaataat acgtactata
       61 atgaaacgta aatatgtttt cttaccacta ttattacttg gtagtgtaag tttaagtagt
      121 tcaccttcgc caagagtaac gcatttcgcg ccgcactatg ccgctccaag cactgagcca
      181 agcggtccgc aaactttacg catttataac tgggaagaat atattagtga agaagatatt
      241 gatgatccag attgtattga tgttttagcg gcctttcaag aatattattt agaaaatcat
      301 ggtgaagaag tcacaattga gtacaataca ttttcaacga atgaagatat gtataatatc
      361 gtcaaactca atggcgcaaa gtatgactta attgccccaa gtgactatat gattcaacgg
      421 atgattcgtg aagatatgct cgaaaagtat gaccttgacg cagaaggtaa ttataaatat
      481 gttcctaatt ataatgaata tgcaagtcca tatttaattg atctttttaa agaagattta
      541 ggaatttatg aatacgcccc agggtatatg tggggaacaa tgggttttct ctataatagt
      601 gaatttaatg acacgattaa tgacgatgtc caaagttgga tgatgttatg ggataaaaaa
      661 tatagtaaga aaacttcgat caaagatagt atgcgcgaag gttatttaat gggacttttt
      721 aaatattacg aagatgaact taatgattta gccgcgcaga gggaagcagg gcttattagt
      781 gatgactact ataacgatga gattagtgac ttacttaatg atgttgaccc tgatacgatt
      841 aaagaagtga ttcacgtgat gaatgaactt aaaaataata tttatgggtt agaagttgac
      901 caaggtaaga ttgatatgat tactgggaaa atcgctgcga acaccgcgtg gagcggggac
      961 gcagtgtatg ccattgaaga agacccaaat gatgtgctcc gttacgctat tccaaaagaa
     1021 ggtagtaata tttggtttga tggctggatt atgcccaagg gcgctaatcg tgtcgtcgca
     1081 caacgctttg tgaactttgt gagtagtccg gaaatggcaa tcgctaatat gtattacatt
     1141 ggttacacta gttttattgc gggggatgaa gtacttgaat atgtgcttga taacgatgca
     1201 gtgtatgagg cagcagaagc agaagaaaca gttgagcttg actttacata tttctttagt
     1261 gatacaatcg aaaatcctag tgacgcctta attacgattg atgcgagtta cgttggtcat
     1321 acattaaccg cgacttatcc gacggaagaa gaaatcgtcc gcagtgtcgt catgcgtgac
     1381 tttggtgata gtaataagct cgtcattgat atgtgggcaa actttaaagc gacggacatt
     1441 caaaactgga tgattatctt tagtgttatc atcgttttag cagttggtag tgccgtcacc
     1501 cttaaccaag tccaaaaaca taaggcagcg cggcgtaagc gcaatttata taaagataaa
     1561 caatgaaaaa aagattagca gtcatcggtg gtggagtaag cggagttacc ttagcgcgac
     1621 ttgtgcaagg ttcctttaat gtcaccatct ttgagcgtga atctagtctc ttacaaaaac
     1681 ttttacgcac gggtaatgga cgcgcgaaca tttataatcg caacatacat tcctttgctt
     1741 ataatgatga acacttttta agtgaacact taccacatat cgttaaaacg cttgatgact
     1801 tatttagtgc ttttaatatt gtcacttaca cggatacaga aggaagagta tatccctata
     1861 gcagaagtgc taaagcactc cgcgcattac tcattaaaga tttaaaggca gaagttaagt
     1921 tagaacataa tgtcgaagaa atcgttaaag ttcgtgataa atatatagtt gatggtgaag
     1981 aatttgacgc tgttgcgtta acaatgggca gtagcgcggg actttttaag tatccgcttg
     2041 ataacggtaa cgcggcgctt ttaagtgctt tagatttaga aacaactgcg ctagtaccaa
     2101 cgattaaaac aattaaagtc aaagaaaatt taaaacgtat tgaacacgaa cgcatcacgg
     2161 caagagtgac gctaatggat gacgaaaatg ttatcgctag tgacgtcggc gaggtgctat
     2221 ttaagaaaga cgggttaagc gggattgtaa gttttatttt aagtagttac cttgaatggg
     2281 ctgtcgttag atatccaaaa cgaaactatt atttaacact tgatttaatg cctgaattta
     2341 gtgcgcgtta cactgccgaa attattaaaa ccactgatga cttagataat gtctttagcc
     2401 ctaatattag tcactatcta aaaggtagaa atgaaccgtt attagatcgg atgaaggctt
     2461 taacctttac tccaatacca aagaacaatc cagaaaacac aagtgcgatg catggtggaa
     2521 ttaagctttc aaaattaaat acggactttg aagtacgcga aagccccaat ctctttgctt
     2581 taggtgaagt cataaacatt gacggaatat gcggtggcta caatttagcg ttcgcttttt
     2641 actccgcaaa agtcgtaagt gacgcactct tacgaaaatt gtaaatatag cgcttccact
     2701 ttatacaatg tataaattac taattaaaac ctagcaaaaa agggctattt tggtcaaatt
     2761 agtagacaat tagtagttat ttttctcgcg aaatcacaaa aaatgaacaa aatttcgtta
     2821 ctgattaatt actactttcg agaacaaatt accgataagt gacaaaagtt gcaaagtcac
     2881 tttttttgtt ttaatcttat ggacagaaat tagaaataat tttcttgaga cacagaggga
     2941 gggtttttta tggtcacaca tattaagaaa agagacggga gattacaaaa gtttaatgct
     3001 ggtaaaattg ctaatgctat caatgcggca tatgggaaca ctaatgacgt gcagtcatta
     3061 ctttatgcag cggaaatcac cagtaattta aacacgaatt acgatgggac aatcgacatt
     3121 gaagtcgtcc aagacgaagt cgaaaaattc ttgatgaaga aagatcccaa cattgctaaa
     3181 aaatatattt tatatagaaa agatagagaa cggattcgta acgctaaaag caccgttttt
     3241 aactataaaa aactcgttga agattattta aatattgccg actggcgcgt taaagaaaat
     3301 tcaaccgtca attattcttt aggtggcctt atcttaagca atagcggcgc tgtaaccgca
     3361 aactattggt taagtgaagt gtatgacgaa gaagtagcaa atgcgcaccg cagcgccgcc
     3421 attcatattc atgacctaag tatgcttagt gcttactgtg cgggttggga tattgaaaag
     3481 attattcgtg atggtgttgg tggcgttgaa ggaaagattt caagtaaacc agcgaaacac
     3541 ttattcacac tcgctaatca acttgttaat ttcttaggca tcatgcaaaa cgaatgggct
     3601 ggggctcaag cttttagtag ctttgatact taccttgcac catttgtaaa agctgataac
     3661 ttaacttacg ctgaagtaaa acaagctatt caatcatttg tgtttggggt caacacaccg
     3721 agtcgttggg ggagtcaagc accgtttagt aatattacac tagactggac tgtccctagc
     3781 gacctcgctg atcgtccagc gattgtcggt gggaaagata tgccctttaa atataaagac
     3841 tgtcaaaaag aaatggatat gattaataaa gcgttccttg aagtaatgat tgaaggtgac
     3901 gctaacggaa gaggattcca gtatcctatc ccaacatatt ccattacgcg tgacttctca
     3961 tgggaagaaa cagaaaacaa taaactatta tttgaaatga cggcaaagta tggtactcct
     4021 tactttagta actatattaa tagtgatatg gaaccaagtg atgtgcggag tatgtgctgt
     4081 cgcttacggc ttgacttacg tgaattacgg aaaaaaggtg gcggcttctt tggtgctggt
     4141 agtaatactg gttcagttgg tgttgtgaca cttaacatgc cacgcttagc ctatctctcc
     4201 cgtactgaag ttgaattctt taatgaactt gataaattaa tggacatcag tgcccgcagt
     4261 ttaaaagtaa agcgtgatgt cgttagtaaa ttactcgaag acggtctctt cccatataca
     4321 aagcgctact taggtaactt tgataatcac ttctctacaa ttggtttagt ggggatgaat
     4381 gaagcgtgtt taaacgctag atggttagga aaagacttag gcgatccaga atcacaagcc
     4441 tttacgatta aagtgcttaa tcatatgcgt gaaaaactta gtgactatca agaagcttat
     4501 ggtgacctct ataacttaga agcaacacca gcggaaagta cagcctaccg ttttgctaaa
     4561 cacgataaag tgcgttatcc cgatattatt actgctaggt taggggaaga cggcgatcct
     4621 ttctatacga actcaagtca cttaccagtt ggttataccg caagtattag tgaagcgctt
     4681 gatattcaag atgaaatgca aacactttat acttcaggga ctgtgttcca tactttcctt
     4741 gggcaaaagt tgccaaactg gcaaaccgca gcccgcttag tgcggaaaat tgctgaaaac
     4801 tatcgcttac catactttac aatgtcacca acatattcca tctgtccaac acatggttac
     4861 ttaactggtg aagttgaaat ttgtccagaa tgtaaaagaa caacagaagt gtattcccgg
     4921 attactggat actatcgtcc gcttaagaac tggaatgacg ggaaaaaagc ggaatttgaa
     4981 aatcgtttag aatacgctga tttcactatt gaaggtggca cagtgcataa tccaaaattt
     5041 aatgcaccaa aagttgaagt aaaagacaca gcggacgcac caattaaaga gcaaaagcct
     5101 accccacata aaggtgcacc aatcatcttc ttaaacaaac aaaataatga ctaaaaaaaa
     5161 gatacgcata gcgggatatg taaacgactc cttggttgat ggaccaggga ttcgttttac
     5221 tatttttacc caaggttgtc cctatcactg tttaggttgt cataatcctg atacatggga
     5281 tccgaagggt ggagaagaag ttgaaataag tacgcttatt gctaaatggc gcaagaactc
     5341 gctgcttgat ggtattacct taagtggtgg tgaaccgctt ttacaaaaag aagcactctt
     5401 accgcttatt aacgctgcga gaaaagacgg acttaatatt acgatgtaca ctggttcaac
     5461 ttatgaagag ttaattcaca gaaacgatcc acttgtcaat gaaatcttaa gtaaagttga
     5521 ttacttaatt gatggtcctt ttattcttca tgaacttaat cttaatttac tctatcgtgg
     5581 cagtaataac caacggatta ttgatttaag aaaaacaaca cctaataaca ttgtccttat
     5641 taatacaatg catgacttta agtaaagaaa gccataatga aaaaggagtg acaagagacg
     5701 cgtcactcct tttgtttata aagtgttttt tctattttct aaacattatt ttttcattgc
     5761 taaacatgac ggtaagcaag taaactccgc cggcagttaa aagtaagtta gaaagcattg
     5821 ttaagataaa cggtacaacg aggaattcac caccaaagag tgagagtaaa atattaaccg
     5881 agttataaat tgggattaag tagagcgcgt tattcgttgc cgcactacca aacatcgtgg
     5941 tgacccctac cgccataatt accatcatga gtggtgctgc tagtccgcta gcttctttaa
     6001 tcgacttagc gtacgcactg atgatgctat ttaatacaat aaagacgagc atcgagacaa
     6061 ttaagacggc aaagatagct aagtaagtac cagcggtaaa cgtcgctaga ctaagttcac
     6121 tccccatcat ttttggtaag ctaagcatta ctccaaggaa actacttgca gcgctgacaa
     6181 gggcgacgat acttaaagct gtaacttttc caagcgctaa agtactcctt ttcactggtg
     6241 taactaagag ggttgcaatc gtaccgcgtt ctttttcgcc agcaatactt tcaggagcaa
     6301 ccgccattgc accggtaaag ataaaggtaa taagtaagaa ggggagcagc cctttaataa
     6361 ttgtagtatc aatcccgcct tctgccgaat gattaatggg aacggcaaca taagtttgga
     6421 gggacgcatt taagagccca gcataagtgc tataagcatt taagcttggg atcactgatt
     6481 catcgtagtg aaaagtaaca actggcggat cattaggatc attcgcgtta tattttgcta
     6541 aaaaatcagt gttataaaca acatataagt caagtttctt ttccgcaatc attgcttcaa
     6601 agtcttcagg agtttcatca aggaacgtaa cttttttaaa cccgacttca ggatcagcga
     6661 tcagtgtttc aatcgcgagg aaccgcgggt catttggtct attttcgaca cggatgacgt
     6721 attcatggtc tttcccttcc attgttgatt gcatcacatt tcccataatt gaatagagaa
     6781 tatacacgat aacaccagga aggattaagg ccgctagcat tcttttatca gtgaagaagc
     6841 gctttaattc ttttttaatg acagtccata agtttttcat taggcaaggt ctcctttcac
     6901 tttcgcatat tcaaggaaga agcgttcctc aagggaatta ggttcactaa atgtgctcat
     6961 tggttcttca aagattaacg ccccatcaat aatgacaccg acacggtcac atattctttc
     7021 gacgagtgag aagatgtgcg tagaaagaat aacagtcttt cctgcttctt tcagcgcgac
     7081 aaggaaatca atgactgttt tcgcagtaat aacatcaagg ccgtttgtcg gctcatcaaa
     7141 gataatgaat tttggatcat gaacgacact gatgacaagt gaaacctttt gtttcatccc
     7201 gctactaagg tcacttactt ttacttcttt aaattcactg atgccgaatt tatcgaaaag
     7261 ttcttgttta cgtttttcaa tcacatcttc aggaacaccg tgaagatgac taaagtaatc
     7321 aaataagtaa ttaggagtga agaagtcgtc taactttaat tcactcgtta aaaagccaat
     7381 ttgactgcga actttacttt gttctttact aatgtcataa ccattaactt taacttcacc
     7441 gttatctttt ttaattaagg tcgagatgat gcgtagtgtt gttgttttac ctgcgccatt
     7501 tggtccaagt aaaccatata tttctccttc ataaacatta aaggagaggt cattaaccgc
     7561 aacacgaact ttcgtatttg ttttttcgat tttttgttgt tttttcgaaa gtttaaaggt
     7621 ctttactaaa tttttaactt ctaagattgg ttgcatagtt cctcctcatt tctttattat
     7681 acataaagaa tatattggat tttgacgtta ttacaattat ttacgagatt tttacagaaa
     7741 ttaggcgaag taatattgta tttattaata gtgggattaa ttattaaacg cttaatttaa
     7801 ttaataaaat aatgacaaca tattgacaat gtaatgatat aataataaaa tgacattgaa
     7861 taggaggaat taaagtatgt ccatgacgaa tttaaatatt aggatggata agaaagtaaa
     7921 agaagaagca gaagcgatat ttcaagaatt gggactcaat atgacgactg ctgttaacat
     7981 atttttacgg acgacaattc gtgaaaaagg catcccattt catttaagtt taagtgtttc
     8041 aaatgttacc actaaagcgg caattgaaga aggaagaaag attgcgctta ctgataatgt
     8101 taaggggtat gataccgttt ctgatttaaa ggcagcattg gaagattaat gtttaaaatc
     8161 aatttaaatt tagtaatctg gtgtttaatt gtagtacgct ttttatcata gttttaataa
     8221 ttaaatcttt ttgttataat tgagtagcgc ttccatagcc aagtggtaag gcaattgact
     8281 gcaactcaat gaccgttggt tcaaatccga ctggaagctc caaaaaaatc tttgtttggt
     8341 aaggtaaaag cgactaaaac aacgttaaaa cgcccttaaa tgggcgtttt ttcattatat
     8401 ggattaaatt agatgattta ttttgtttgt taaatttgtg atcatattta taaaagtatc
     8461 aaaattacat ttccgttaca gtggtttgaa cctaaaatac tattttccgt tacagtggtt
     8521 cgaacctaaa taaaaataaa aaaaacaatg tctttattac cattaccaac ccttaaatta
     8581 attgatgaat gtaataaagg ataatccaga atatctgttt ccaataaata aaatcttttc
     8641 tagtcttaat tgtgcttagt tgcttgtact gataccctaa atgtagacac acaataaaat
     8701 catgtggcca catgattt
//
LOCUS       DAOSTA010000038         8656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_7751_length_8656_cov_11.538426, whole genome shotgun sequence.
ACCESSION   DAOSTA010000038 DAOSTA010000000
VERSION     DAOSTA010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8656
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7751_length_8656_cov_11.538426"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..2569
                     /locus_tag="PLU08_04090"
     CDS             77..2569
                     /locus_tag="PLU08_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013371600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOQ70902.1"
                     /translation="MKKRLLSLFVISVLFSNVPSTSTKIVETNAQTGITVHYRGDYTN
                     PHIYYWNPQPAIITTPSWPGEKMIKDEGSDWYTKSWPELTAINLIFNNGGQPQTSDLS
                     RTTGEYWWVGQGTGGKWYTSDPDTITEPEYTDTGKLVIHVKSPHSVPKIKYYDSVPSG
                     ISTPNAINMTAEKEGWYRFGFNKISSIKAKFTIDGSESAEYFFTNGEWYLENGVLTDF
                     KPKVTSGKSDFREETIYFLMTTRFYDGDTSNNVHCWDDAKAGNLASDPAWRGDFKGLI
                     QKLDYIKALGFSAIWITPIVENASGYDYHGYHALDFTQVDPRLESPNATYQTLINETH
                     ARGMKIIQDVVFNHSGNFGEKNIFPLFKKDYTKADTVSNLIKTDPHNLLGSDYDALDG
                     GRQYSRRINAMKEDANDFRHIYHHEKSLGWEDYNVQTGQIAGDCVDLNTENPYVSDYL
                     INAYNRYIDMGVDAFRVDTVKHISRLTFNNVFNPAFQNRGGDDFFMFGEVATRYRQVW
                     NNGNPAISTPFYTWKESKSYPWATREEREASTYQHWQDNANPATQPSSNNHLLDGNNY
                     RQVDYSKKSYMNVIDFPMHWNFKNANDAFNVAVGNDYTYADATWNVMYVDSHDYAPDG
                     APEGQRFAEPQSVWAENLSLMFTFRGIPTIYYGTEIEFQKGKPIDVGPNAPLSETGRA
                     YFGNHIEGSLTVTDFGKYTNANGAVATTLNHPLAKHIRNLNLIRRNIPALQKGQYSTA
                     GINGGLAFKRRFTDSTTDSFALVTISSSATFNNIPNGTYVDAVTGEAKVVTNGSLSVN
                     VSGKGNLRVFVLTTSKTPAPGKIAESGLTYIK"
     gene            complement(2576..3460)
                     /locus_tag="PLU08_04095"
     CDS             complement(2576..3460)
                     /locus_tag="PLU08_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ70903.1"
                     /translation="MFKSKFAIDPSLLHKDSVYTLNNGVKMPVIGFGTWQAKDGEEAY
                     ESVKAALRVGYRHIDTAEAYHNEESVGRAIRDSGIPREEIFVTTKLTNTHLTYEDAKQ
                     AIDASLKRLGLDYVDQYLIHWPNPINARTFYKKRNAEVYRAMEEAYFAGKIRSIGVSN
                     FHPHHLKALLKTIKVKPATNQIYVSPSDTQPKVVAFNEKHNILTVAYSPLGTGRLLGL
                     NELNQVGTKYNKRPAQVAIRWSLEHGYLPLPKSTNASRISENFDVFDFKLSKEDIKLI
                     NSLKGKAGKAPDPDHVIW"
     gene            3625..4176
                     /locus_tag="PLU08_04100"
     CDS             3625..4176
                     /locus_tag="PLU08_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOQ70904.1"
                     /translation="MSDPKVSRIIALLNELKRGNNDNFAEFYELTKYQIFYSLLSLTK
                     NHELAEDILAETYLKFLDNLKRIKANQNPLGFLLVTARNLAYDHFKKENRVSYIEDYL
                     LESDIGASTTDKYDDSDRLLARMQEILNETEYQVVVLYVLSELTHKEIAEYLDKPLGT
                     ITWTYSNAIKKLQKGLSDYARDE"
     gene            4169..5233
                     /locus_tag="PLU08_04105"
     CDS             4169..5233
                     /locus_tag="PLU08_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70905.1"
                     /translation="MNDDKIKKIIQENVTKPEIKLSAAQILAMHEERKRAKEKPKRIP
                     FFKRPTFKYSLGFLSAALVIGITVYALRDTIVPPIISTSEGGETSTPSSGEVSTPSVP
                     VSEHNSDKIPHGKEGEFVFMTSSALNYAPEGSIPGGMGLIKPWYSISLSDPEKLELEA
                     ALDETLPLVSDFFSLDKGFNYQKNKGNYAGTYGTYTHEFIINEYTRIIGNVEIEEDDT
                     ETETELEGEIIIHDTHFHFEGETEIDTADNETDISLKIEYNDDSYLEIESENEADEQT
                     FKYKLYLDGDEKLNVEIKSYNTHGNGNGGRCAEVKTKYNNEDYEFYITKKSGNYVIEY
                     LEMIIIATETEDKKFIYSYE"
     gene            5324..6367
                     /locus_tag="PLU08_04110"
     CDS             5324..6367
                     /locus_tag="PLU08_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70906.1"
                     /translation="MKKTFTLMTLALGATLVLGGCTKPNTEATAVIKSQTMAFEATTA
                     LTLVGGFETVPEVKALQKSLAAEGVAEPDIPVATLDILFNNGVDFQINREDSDLAGYT
                     YVDTISFNIGTDSRIEYKLYYNLETAEEEIDDDSDDDELLNNEVASKARYGENKHNGK
                     DDDDDDTVCNGGGTCTNDGEEGDHNKEQDRNRNRNKDKHYRVTGLALVGEEEYKFMSK
                     TTSEVDADETETKLRFMLWKDDNNFISIKQEIEVEGTPGTPDYEYEEEFKYLVVENGE
                     VVKSFKLELENEDNELKLEVRIDGVKYEVEYEVIDDRTFIHVKVQGTEYTYEKVITTN
                     EETGEVTVSYVLQ"
     gene            complement(6431..6622)
                     /locus_tag="PLU08_04115"
     CDS             complement(6431..6622)
                     /locus_tag="PLU08_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70907.1"
                     /translation="MMKKGAIGAANVVKEKLRIENIINATLKPSADYRLDDFLIDDKA
                     LKTEGEGRNLVYVLNNRTY"
     gene            complement(6912..8252)
                     /locus_tag="PLU08_04120"
     CDS             complement(6912..8252)
                     /locus_tag="PLU08_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008402275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ70908.1"
                     /translation="MYLKRKIDEYLVNWKKDNNKMPLIVKGARQVGKTESIKKFAENN
                     YENVIYINFVEEPIYKGITADGYKTDAIIKIMSRIDPLKVFTPYKTLIFFDELQDFPD
                     ITTALKFFKLDGRFDVICSGSLLGINYRIIESNSVGYKVDYEMNSLDFEEFLWAKGYS
                     DDVVRDMLKHMCELKPFNDVDMKIFNDLFLDYAILGGMPAVVRLYIENGTFEKTLELQ
                     KQLILDYKEDVRKYVVGLDQTRILNVFNHIAPQLAKDNKKFQITKVGKNARFADYRGC
                     IEWLKDSGVINVCYGLNYPELPLKGNYDLNKYKIYFFDTGLLVAQLDEEAQLDLRANK
                     NLGVYKGALYENIAAEALVKQSYELYYYKRNDATLEQDFFVRDIDSLIPLEVKANGGT
                     SKSLDTLISSDKYEDIKYGIKFAKSNIGYKDNIYTFPHFCMFLLKRYLNFKMEK"
     gene            complement(8579..8654)
                     /locus_tag="PLU08_04125"
     tRNA            complement(8579..8654)
                     /locus_tag="PLU08_04125"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8619..8621),aa:Ala,seq:cgc)
BASE COUNT         2967 a         1543 c         1516 g         2630 t
ORIGIN      
        1 caaaacatta ttttcaactt accaaaataa taatgtcatc ctttgttata atgaagttag
       61 cgttggagaa acacttatga aaaaacgatt gttatctttg tttgttatta gtgtcctttt
      121 tagtaatgtg ccaagtactt ccactaaaat agtggaaaca aatgcacaaa cagggattac
      181 tgttcattat cgcggtgact acactaatcc gcacatttat tattggaatc cgcagcccgc
      241 tattattact acaccaagtt ggcctggtga aaagatgata aaagatgaag gaagtgattg
      301 gtacactaaa tcgtggcctg aattaactgc tattaactta atatttaata atggtggaca
      361 gccacaaact agtgatttat cacgcactac tggcgaatac tggtgggtcg ggcaaggcac
      421 gggtggtaaa tggtacacaa gtgaccccga taccattacc gaaccagaat acactgatac
      481 tggtaaatta gtaatccatg ttaagtcacc acacagtgta cctaaaatta agtattatga
      541 cagtgtccca agcggaataa gtacaccaaa tgccataaat atgactgctg aaaaagaggg
      601 atggtaccgc tttggcttta ataagatttc aagtattaaa gctaaattca caattgatgg
      661 tagtgaaagt gctgagtact ttttcactaa tggcgaatgg tatttagaaa acggagtact
      721 cactgacttt aaaccaaaag taacaagtgg taaaagtgat tttagagaag aaacaattta
      781 cttcttgatg acaacaagat tttatgatgg tgacacatct aataatgttc actgttggga
      841 tgatgctaaa gcaggtaatt tagctagtga tccagcgtgg cgcggagact ttaaaggctt
      901 aattcaaaaa cttgattaca ttaaagccct tggttttagt gcaatttgga ttacaccaat
      961 tgtcgaaaat gcttcgggct atgattatca cggttatcac gctcttgact ttactcaagt
     1021 tgatccgcgc ttagaaagtc caaacgcaac ttatcaaact ttaattaatg aaacacatgc
     1081 acgcgggatg aaaatcatcc aagatgttgt ctttaaccac agcgggaact ttggagagaa
     1141 aaacatcttc ccactattta aaaaagatta cacaaaagct gacaccgtta gtaacttaat
     1201 taaaactgac ccgcataatt tacttggtag tgactacgac gcacttgatg gtggtagaca
     1261 atatagtagg cgtattaatg caatgaagga agacgctaat gacttccgcc atatttatca
     1321 ccatgaaaaa agtcttggct gggaagatta taatgtgcaa accggacaaa ttgctggaga
     1381 ctgcgttgat ttaaacactg aaaatcctta tgttagtgac tacttaatca atgcttataa
     1441 tcgctatatt gatatgggag ttgatgcttt tagagttgat actgttaaac atatttcacg
     1501 cttaaccttt aataatgttt ttaatcctgc ttttcaaaat agagggggtg atgacttctt
     1561 tatgtttggg gaagtcgcca ctcgttatcg ccaagtatgg aataatggta accccgcaat
     1621 tagcacaccg ttctacacct ggaaagaaag taaaagttat ccgtgggcaa cacgagaaga
     1681 gcgggaagca agtacatatc agcactggca agataacgct aatccagcaa cacaacctag
     1741 cagtaacaac catctgctag atggtaataa ttatcgacaa gttgattact caaagaaaag
     1801 ttacatgaat gttattgatt tcccgatgca ctggaacttt aagaacgcta atgacgcctt
     1861 taatgtcgca gtcgggaatg attatactta tgctgatgca acatggaatg taatgtatgt
     1921 tgattcgcat gactatgcac cagatggcgc tcctgaagga caacgctttg ccgaaccaca
     1981 aagtgtatgg gcggaaaatt taagtctgat gtttactttt agaggtatcc caacgattta
     2041 ttacggaaca gaaattgaat tccaaaaagg aaaaccaatt gatgttggac caaatgcccc
     2101 acttagtgaa acaggtcgcg catattttgg taatcatatt gaaggctcac tcactgtgac
     2161 tgattttggt aagtatacga acgcaaatgg agcagttgca acaacattaa atcacccatt
     2221 agcgaaacat attcgtaact taaacttaat tagaagaaac atccccgctt tacaaaaggg
     2281 acaatatagt actgctggta ttaatggcgg tttagcgttt aaacgccgat ttactgatag
     2341 caccactgac agcttcgcac ttgtcacaat ttcaagtagt gctacattta ataatattcc
     2401 aaatggtact tatgttgatg ctgttactgg tgaagcaaaa gttgtcacta atggttcttt
     2461 atccgtgaat gtatctggta aaggcaattt aagagtgttc gttttaacta cttctaaaac
     2521 acctgcacca ggcaaaatag cagaaagcgg attaacttat attaaataat tatatttacc
     2581 aaattacatg atctggatca ggggctttgc ccgcttttcc ttttaatgaa ttaattaatt
     2641 taatatcttc tttacttaac ttgaaatcaa aaacatcaaa gttttcacta atgcgtgatg
     2701 cgtttgtgga tttaggtaac ggcaaataac catgttctaa actccatctg atcgctactt
     2761 gagcgggacg cttattgtat tttgttccaa cttgattaag ttcatttaac cctaatagtc
     2821 gaccagtgcc aagtggagaa taggctactg tcaaaatatt atgcttctca ttaaaggcga
     2881 caactttagg ttgtgtatca cttggactta catatatttg atttgtagcg ggttttactt
     2941 taattgtttt aagtaatgct tttaagtgat ggggatgaaa gttactaaca ccaattgagc
     3001 gaattttccc agcaaaataa gcttcttcca tcgctctata tacttctgca tttctctttt
     3061 tataaaaagt acgagcatta attggatttg gccagtgaat taaatattgg tcgacataat
     3121 caagccctaa gcgctttaaa cttgcatcaa tcgcttgctt tgcgtcttca taagttaaat
     3181 gagtatttgt taatttagtg gtaacaaata tttcttcgcg cgggataccg ctatcacgaa
     3241 tagcgcgccc aacactttct tcgttatgat aagcttcggc agtgtcaata tggcgataac
     3301 caacacgcag cgctgctttt acgctttcat aagcttcttc gccgtctttt gcttgccagg
     3361 tgccaaaacc gatgacgggc atcttcactc cattatttag tgtataaacg ctatccttgt
     3421 gtaaaaggct aggatcaatt gcaaattttg atttaaacat ctgacccctc ctataaatat
     3481 tttaacaaat aaattatact tttttcagag aatgtgtgat tttataagac aaagtcttaa
     3541 gtttgttata atagtcgtgg aaaaaaattt aaatattacc aataaaatga tgaacttaat
     3601 cgtattaata gtgaggaagt gcaaatgagt gacccaaaag tcagtcgtat tattgcatta
     3661 ctcaatgaat taaaacgcgg gaataatgat aacttcgcgg agttttacga actcacaaag
     3721 tatcaaatct tctattcgct attatcgctg acgaaaaatc acgaactcgc ggaagatatt
     3781 ctcgccgaaa cttatctaaa gtttttagac aatctcaaac gcattaaggc caatcaaaat
     3841 cctcttggct tcttgcttgt gaccgctaga aacttagcct acgaccactt caaaaaagaa
     3901 aatcgtgttt cttatattga agattatctc cttgaaagcg atattggtgc gagcacaact
     3961 gacaagtatg acgatagtga tcgcttactc gccaggatgc aagaaattct aaacgaaaca
     4021 gaataccagg ttgtcgtgct ttatgtttta agtgaattaa cacacaaaga aattgccgaa
     4081 tacttagata aaccattagg caccattaca tggacatata gcaacgctat taaaaaatta
     4141 cagaaaggat taagtgatta tgcacgggat gaatgacgac aaaattaaaa aaatcattca
     4201 ggaaaatgtg acaaaacctg agattaagct ttccgctgct cagattttag ccatgcatga
     4261 agaacgtaaa cgcgctaaag aaaagccaaa acgcattcct ttctttaaac gtccaacatt
     4321 taaatatagc ctaggtttct taagtgcagc gcttgtaatc gggattacag tctatgcttt
     4381 aagagacact attgtccccc caattattag cacaagcgaa ggtggcgaaa cttcgacccc
     4441 aagcagtggt gaagtctcca ctcctagtgt accagtaagt gaacataatt cggataaaat
     4501 cccacatggt aaagaaggtg agtttgtatt tatgacttct tctgccctta attacgcccc
     4561 tgaaggctca attcctggtg gaatgggatt aattaaacca tggtatagta tctcacttag
     4621 tgatcctgaa aaattagaac tagaagcagc gctagatgaa acgctaccct tagtgagtga
     4681 cttctttagt cttgataaag gttttaatta tcagaagaat aaagggaatt atgcgggaac
     4741 atatggtaca tacacgcatg aatttattat taatgaatac acacgcatta ttggtaatgt
     4801 tgaaattgaa gaagatgata ccgaaacgga aacggaactt gaaggtgaaa tcattattca
     4861 cgacacacat ttccattttg aaggcgaaac agaaattgat actgctgata acgaaacaga
     4921 tattagttta aagattgaat ataatgatga ttcatattta gaaatcgaga gcgaaaacga
     4981 agctgatgaa caaacattta aatataaact atatcttgat ggcgacgaaa aacttaatgt
     5041 tgaaattaaa agttataaca cgcatggtaa tggtaatggt ggtcgttgcg cagaagtaaa
     5101 aacgaaatat aacaatgaag attatgaatt ttacatcaca aaaaagagcg gtaattatgt
     5161 cattgaatat cttgaaatga ttattattgc aacagaaact gaagataaaa aatttattta
     5221 ttcttatgaa taaaaaataa aataaaaata atttttaaat aattccaata aaaaagaatt
     5281 ttaattcgta tttattgtga acggaaaaaa ggaggaacta cttatgaaaa aaacgtttac
     5341 actcatgact ctcgctcttg gagcaacact cgtgcttggt ggctgtacaa aaccaaatac
     5401 tgaagccaca gccgtaatta aaagtcaaac aatggccttt gaagccacga ccgctctcac
     5461 actagtgggc ggattcgaaa ctgtaccaga ggtgaaagcc ttacaaaaat cacttgccgc
     5521 ggaaggcgtt gccgaacctg atatcccagt tgcaacactt gacatcttat tcaataatgg
     5581 tgttgacttc caaattaatc gtgaagatag tgatttagcg ggttatacct atgttgatac
     5641 gatctccttc aacatcggaa cagatagccg cattgaatac aaactctatt acaatttaga
     5701 aacagcggaa gaagaaattg atgatgatag tgacgatgac gaattattaa ataatgaagt
     5761 cgcaagtaaa gcacgctacg gcgaaaacaa acataatggc aaagatgacg acgacgatga
     5821 cacagtctgc aatggcggtg gcacatgcac taacgacggc gaagaaggcg accataataa
     5881 agaacaagat cgcaatcgta atcgcaacaa agacaaacac taccgcgtta ctggcttagc
     5941 gctcgttggc gaagaagaat ataaattcat gtctaaaaca acaagtgaag ttgacgctga
     6001 cgaaaccgaa acaaaattac gctttatgct ctggaaagat gacaacaact tcatcagcat
     6061 taaacaagaa atcgaagttg aaggcactcc aggaacccca gattatgaat acgaagaaga
     6121 attcaaatac ttagtcgtag aaaacggcga agtcgtgaaa agcttcaaac ttgaacttga
     6181 aaacgaagat aatgaattaa aactcgaagt tagaatcgat ggcgttaaat acgaagttga
     6241 atatgaagta attgatgatc ggacattcat ccacgtcaaa gttcaaggca ctgaatacac
     6301 ctacgaaaaa gttattacca cgaacgaaga aactggtgaa gtaactgtta gttacgttct
     6361 ccaataagca aagtcattaa tagagccata gtaataatcg gaccatggca acatggtcct
     6421 ttttaattta ttaatatgtt ctattattta gaacatatac taggttccgg ccttcgcctt
     6481 cagtttttaa tgctttatca tcaattaaaa agtcatctaa acgataatcc gctgaaggtt
     6541 taagggtagc attaattata ttttcaattc gtaatttctc ttttactaca ttagcagcac
     6601 caatagcacc ttttttcatc ataaccaata ataatttcgc caccatcaaa attagcgaac
     6661 gcactaatag tttttaagag tgtgtctgaa tattggcttt ttaattctaa ctttttagtt
     6721 tatttatgtt gttgtttcat agcacttcac cttctaggct aattagaatc taaaacaact
     6781 atgtttgcaa cgaataattt tttctcgttg caaatatttg taaatgaaac gacctgtggt
     6841 cgtgttaaat gataaaaata ggagctttgt ccgctattct tttgtttttt ttagaattta
     6901 ttatttaata gctatttctc cattttaaaa ttgaggtatc tctttaataa aaacatacaa
     6961 aaatgcggga atgtataaat attatcttta taaccaatat tacttttagc aaatttaatg
     7021 ccgtatttta tatcttcata tttgtcactg ctaataagag tatcaaggga ttttgatgtc
     7081 cctccattag ccttaacttc aagtgggatt aaactatcaa tatcgcgtac aaagaagtct
     7141 tgttctaaag tagcgtcatt acgcttataa taatataatt cataactttg tttaactaat
     7201 gcttcggcgg cgatattttc atataatgca cccttataga cacctaaatt tttattagcg
     7261 cgtaagtcaa gttgtgcttc ttcatctaat tgtgcaacaa gtagtccagt atcaaagaaa
     7321 tatattttat atttattaag atcataattg cctttaagcg gaagttctgg ataatttaac
     7381 ccatagcata cattaataac ccccgaatct tttaaccatt caatacagcc gcggtaatca
     7441 gcaaaacggg catttttgcc aacttttgta atttgaaatt ttttattgtc tttcgctaat
     7501 tgtggtgcaa tatgattaaa aacattcaaa atgcgtgttt gatctaaacc aacaacatat
     7561 ttgcggacat cttctttata atccaaaata agttgttttt gtaattcaag tgttttttca
     7621 aatgtaccat tttcaatata aagtctgaca accgcaggca tccccccaag aatagcgtaa
     7681 tctaaaaata aatcattaaa gattttcata tcaacgtcat taaatggctt caactcacac
     7741 atatgtttta gcatgtcacg cacaacatca tcactatagc ctttggccca caaaaattct
     7801 tcaaaatcga gtgaattcat ttcataatct actttatagc caacgctatt actctcaatt
     7861 atcctataat taataccaag cagtgaaccg ctacagatga catcaaagcg accatctaat
     7921 ttaaaaaact tcaacgcagt ggtgatatca ggaaaatctt gtaattcatc aaaaaagatt
     7981 agtgttttat atggggtaaa tactttaagc ggatcaatcc gtgacataat tttaataatc
     8041 gcatctgttt tatacccatc cgcagtaata cctttatata ttggttcttc cacgaaatta
     8101 atataaatga cattttcata attattttcg gcaaattttt taattgactc agtcttacca
     8161 acttgtcgtg cacctttaac aattaaaggc attttgttat tatccttttt ccaattaact
     8221 aaatactcat caattttacg tttaagataa atcataatca tccctcatca tcattaaatt
     8281 tgtaattcaa tattaacact ataatctata tataacaagc ctttttacaa aaaaaggagc
     8341 gagatttttc caaaatttac aaaaaaagga gcgacttttt ggcagaattt acaaaaaaat
     8401 gagggagttt tatatttaaa aacatgtaaa cattcatata atcaatattt ttcgtcgctt
     8461 atctaattaa tgacacaatt ttataaattt tgcttctttt ttggttttca aaataaatgg
     8521 tttttgttat taataaataa aaaaatagct ccctgagcgg aagctaatac ttttactttg
     8581 gtggagccga cgaggatcga actcgctacc tcatcgttgc gaacgatgcg ctctcccaga
     8641 tgagctacgg ccccaa
//
LOCUS       DAOSTA010000039         8645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_7769_length_8645_cov_9.447497, whole genome shotgun sequence.
ACCESSION   DAOSTA010000039 DAOSTA010000000
VERSION     DAOSTA010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7769_length_8645_cov_9.447497"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1424)
                     /gene="ugpC"
                     /locus_tag="PLU08_04130"
     CDS             complement(201..1424)
                     /gene="ugpC"
                     /locus_tag="PLU08_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOQ70909.1"
                     /translation="MAKVKLSGIYKRYEGNKNFSVSDFNLEIGEKEFIVFVGPSGCGK
                     STTLRMIAGLEDITDGELYIDGVLVNDLPPKDRGIAMVFQSYALYPHMTVFDNMAFGL
                     KVQRLPKDEIKRRVHEAARILEIEHYLQRKPRALSGGERQRVALGRAIVRQTEVVLMD
                     EPLSNLDAKLRVQMRSEIIRLHETLGSTTIYVTHDQTEAMTMASRIVVMKDGFIQQVG
                     SPIEIYHNPANLFVASFIGSPPMNFFNVVVQKDHLEFVHYEEREEETEDGEDKVELVS
                     VKERVVDGKLPIPASLAKKLKGYVGKEVVFGVRPENVNYATSEGGREYADFKQNFTVE
                     IAELLGNEYIVHSKLFGQKIISKLLVDPANAIHIGAKLDLVIDFDKVAFFKVDTEAEA
                     AELPSAELNKRIELK"
     gene            complement(1522..2184)
                     /locus_tag="PLU08_04135"
     CDS             complement(1522..2184)
                     /locus_tag="PLU08_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOQ70910.1"
                     /translation="MKSIAIIGLSRFGLSLVEAFSKLDVELVAIDMDEEQVRKAGDFI
                     QNVFIADSTSIESLRAAGIANVDHAIVAIGQNERINLTTSIISVIKLKELGIKEITAR
                     ADEEDYAEVLTLVGATNIVMPLDIAAERIANKIAAGNVLDYFNVRRDYDVYEVKISDD
                     FEPLPIINLDSRTRYKINILLIERNKVLYVPTKDTILEPGDEIFVFGKKQDIPKVLSV
                     FN"
     gene            complement(2194..3534)
                     /locus_tag="PLU08_04140"
     CDS             complement(2194..3534)
                     /locus_tag="PLU08_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOQ70911.1"
                     /translation="MNKKEKKLKPGIKKKMSPYLIIVLSFLSVIIIGALLLSLPFAKQ
                     EGVTLSFLDTFFISTSAVTITGLSPIHNLSTTFSVFGKVVIALLTQIGGLGIITLSVF
                     VMILIGMKIGISNRVLLQTNLNSPSMAGVVNLIKKIVIFTLIVEFIGFLINLAVFIPL
                     FPLPKAIGISAFHAIASFNNSGFDILGDSSLINYQDNILLLINTALMIMIGGLGFIAI
                     QDIFTKRSIKKLSIHTKIVIKVNLFLWIVGTLVIKLAQIGGESTSWLTAFFMSVSART
                     AGFAAEKLVNFHSLSLLMLICLMFIGGSPSSTAGGIKTTTFYTLTKSMISYGLGKPTT
                     TYNRVIDQESKNKAFLLLFTALTGVVIGTALILFFDNLTIGRALFETVSALSNVGLSV
                     GTTATLSSASKVVLIVLMFAGRVGPLTIMSLMNRDWYKIGAHPVDYLEEKIIIG"
     gene            3665..5164
                     /locus_tag="PLU08_04145"
     CDS             3665..5164
                     /locus_tag="PLU08_04145"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOQ70912.1"
                     /translation="MKKRLMTLPLIMLLLSGLKSPISNIEKLNAATPMPTTIVFENNT
                     DAEVSAYYTGVEGKTGDELLEFLYSVIKDHNEYDYENNTHRYIYKIIDRNWELDAIDT
                     VSPANLENFDYVNDNGFIHKLYADYNDDINTADRFKNEGASRVSFDREHIWPQSLGAF
                     GRTGGAGSDFHSLWPSDVKANQQAHSNNNYGVPTSNITNYQSDHDTYVGRNGNVEGYT
                     SKVFEPLDEYKGDIARAMFYMPARYYEYIDKLHPKLELVNGSPAAREASATLTGQAGC
                     LETLLQWHEEDPVSEYEIRRNNLIANNYQGNRNPFIDYPQWARIAYDEGYIGTGATNA
                     LGLSSAAGLRDPFEDVTLKSISIDTTNVKKEYALNEGYDPTGLVVTAHYENGGKRKVA
                     DFTTSMAKGEPLTKGGTKTITVSVTHEGVTKTASYNIEVKEPLPIYVYIGVAAVVLLI
                     IIIYIFSAKSKKAKKKRASNVKKVIKKATKSSSSTKSNSKRKTTKTKKK"
     gene            5285..7492
                     /locus_tag="PLU08_04150"
     CDS             5285..7492
                     /locus_tag="PLU08_04150"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOQ70913.1"
                     /translation="MKFNKLISVSILALLISGTSLTTTKQKEKVSAATPMPTNINMTN
                     RTDAEVSAYYEGVNGKSGDALLAFLYTKIKNHNEYNYESVTHRTIYKIIDRNWALDAL
                     DPNGPANTSNFNYSTDNGFIRKLYADYNDNIATADRFKNDGASRVSFDKEHIWAQSLG
                     NFGRTRGAGSDFHSLWPADVKGNQDAHSNYNFAVPKTSITTYKNDYGTYVGRNGYISG
                     YSQKVFEPLDQYKGDIARAMFYMPARYYEYIDALHPKLELVNGSPAAVTASSSVTGKA
                     GDLATLLEWHELDPVDEYEIRRNNLIANNYQGNRNPFIDYPQWARIAYDPTYTGAGAT
                     NAPETSSVGTNPGGLGETVLSHITVDTTDAKTTYFLGDDFTTNSLIVTAHFSDETSLP
                     VLDYTSSIANGTALNTPGNQSVTISYTHEGVTKNTSYTILVKEFVVSDYLFISEVYGG
                     GGNAGAEYKTDFIELYNYADFPLSLEGYSVQYASAAGTSWQVTNLSGTIAPKSFYLIE
                     EASGSGGTRNLPLAETVGSIPMHAKSLKLALVNSTTALSGADPTSDTSIIDFVGVGTA
                     NAYLGLTAAPEISNTMSAQRKFIIEGIAPPVEKSNGTDFIAALPTPTNAALTIAVDIR
                     AEGDTPNQCLTLYSPYKSRVLSLSPSQLNYFKTVVAGEEERLTDGRARYEAWALHLGD
                     ALPYEYNENFGLGNTNKQTFTSDIVMLVLVIALFGLSIGFYFIKSKQNTAKTH"
     gene            complement(7569..8555)
                     /locus_tag="PLU08_04155"
     CDS             complement(7569..8555)
                     /locus_tag="PLU08_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70914.1"
                     /translation="MKNKLFSTVMLLVLLLTGCKEGSSSITKSEEPSAPSTETSVVIE
                     DSSEAASEYAGLIGMDTYNDWPSAAIDEYLLSHNVEEKVPALPNVTRSYVELTLDDPN
                     FEHYLTVFIPGTNQAEDYTAILQAVNYQVVYYEEDDYYIGLNSETTIQIDYFYVTGDA
                     DYPDGMFIFIEALATNGGGTIVTPEGIPSVIDFATEDQITKRDALQSIWTTESGVTMT
                     IDKGASTVNVGNLTTQNEVGYFTNPLRIYAGQVVTFTVPAEVQINAVLLETAGAWEKG
                     DSVLAVTNSAPETVDFIAEGDNVLFTLFSPSNTLTFSLAAQARFNKITVFVI"
BASE COUNT         2836 a         1539 c         1543 g         2727 t
ORIGIN      
        1 tctcctttct cattgttttg tgggagagcc taaaaatgct aacgatagtt aagctctgcc
       61 aatatttaag tttagacata gtcattatag taattatttt atattttgac aaatgatata
      121 tgtatataat ttggaaacct tttgatctta atcaaaaaaa gctcctgtta gcggagcttt
      181 ttctcgaatt ttgttgatta ttacttgagt tcgatacgtt tatttaattc agcgctcggt
      241 aattcggcag cttctgcttc ggtatcaact ttgaagaaag cgactttgtc aaagtcaatt
      301 actaagtcaa gttttgcccc aatgtggatt gcattagcgg gatcgacgag taatttcgaa
      361 ataatttttt gaccgaataa tttggagtga acgatatatt cgttaccaag taattcagcg
      421 atttcaacag tgaagttttg tttaaagtca gcgtattcgc ggccaccttc gcttgtggca
      481 tagttgacat tttctggacg gacgccaaag acgacttcct tcccaacata acccttaagt
      541 tttttcgcta aactagcagg aattggtaat ttaccatcaa cgacacgttc tttaacactg
      601 acgagttcga ctttatcttc gccatcttca gtttcttctt cgcgttcttc ataatgaacg
      661 aattcaagat ggtctttttg cacaacaaca ttaaagaagt tcatcggtgg ggaaccaatg
      721 aaactagcga cgaataagtt cgctggatta tgataaattt caattggtga accaacttgt
      781 tggatgaatc cgtctttcat aacaacgata cgtgaggcca ttgtcattgc ttcggtttgg
      841 tcgtgagtaa cataaattgt tgtactacct aatgtttcgt gaagtctaat aatttctgaa
      901 cgcatttgca cacgaagttt cgcgtctaag tttgataaag gttcgtccat taatacaact
      961 tcagtttgac gaacaattgc acgtcctaaa gcaacccgtt gtctttcacc accagagagc
     1021 gcacgtggtt ttctttggag ataatgttcg atttcaagaa tccttgctgc ttcatgaaca
     1081 cggcgtttga tttcatcttt tgggagacgt tggactttta gtccaaatgc catgttgtca
     1141 aacacagtca tatgcggata aagtgcatag ctttggaaaa ccattgcaat tccgcggtct
     1201 ttcgggggta agtcattaac gagcactccg tcaatgtaaa gttccccatc ggtaatatct
     1261 tctaacccag caatcatccg taaagttgtg gacttaccac agcctgatgg accaacaaaa
     1321 acaataaatt ctttttcacc gatttctagg ttaaaatcac taaccgaaaa gtttttgtta
     1381 ccttcgtaac gtttgtaaat gcctgataat tttactttgg ccataataaa actccttctt
     1441 caattaattt aatcatagca cacattttta aatttgtgcc aatcgatgtt atatttaaaa
     1501 ttttcataac caagaagaag attaattaaa gacactaaga acctttggaa tgtcttgttt
     1561 tttcccaaaa acaaaaattt cgtcacctgg ttctaatatc gtatcttttg ttggaacata
     1621 gagcacttta ttgcgctcga ttaacaatat atttatttta tagcgggtac gtgagtcaag
     1681 gttgataatt ggaagtggtt caaagtcatc acttatttta acttcgtaaa catcgtaatc
     1741 gcgccggaca ttgaaataat caagaacatt accggctgct attttattag cgattctttc
     1801 cgctgcgata tcaagtggca taacgatatt agtcgcacca actaatgtta aaacctctgc
     1861 gtaatcttct tcgtcagcac gagcggtaat ttctttaatg cctaattctt ttaatttgat
     1921 gactgaaata atgctagtag ttaagttgat tctttcattt tgtcctatcg cgacaattgc
     1981 atgatcaaca ttagcgatac cagcagctct taaagactca atactagtgg aatcagcaat
     2041 aaaaacattt tgaataaagt cgccggcctt acggacttgc tcttcgtcca tatcaatagc
     2101 gactaattca acatctaact tgctaaaagc ttctactaaa cttaagccaa atctacttaa
     2161 accaataatt gcaatacttt tcataaatat attttaacct ataattattt tttcttctaa
     2221 ataatctact ggatgcgctc caattttata ccagtcgcga ttcatcagtg acataattgt
     2281 tagcggacca acccttcctg caaacataag tacaattaaa acgactttgc tagcgctgct
     2341 tagtgtcgcc gtagttccga ctgataagcc cacattagac aatgcagata cagtttcaaa
     2401 aagagctcgt ccaatagtta aattatcaaa gaataagatt agcgcggtcc ctattacaac
     2461 gccggttaat gcggtaaaca gcagcaaaaa cgccttattt tttgattctt ggtcaataac
     2521 acggttataa gttgtagtag gtttcccaag tccgtagcta atcatacttt ttgtaagagt
     2581 ataaaaagtt gttgtcttta tacccccagc agtggaacta ggtgaaccgc cgataaacat
     2641 taaacaaata agcattaata aacttaaact atggaaattt actaattttt cagcagcaaa
     2701 accagcggtt ctcgcgctca cgctcataaa gaaagcagta agccaacttg tcgattcacc
     2761 gccaatttgc gcaagtttga tgactaaagt accaactatc cataagaaaa ggttaacttt
     2821 aattactatt tttgtatgga tgcttaattt cttaattgaa cgttttgtaa atatatcttg
     2881 gatagcaata aagccaagac caccaatcat aatcatcagt gcagtattga ttagtaagag
     2941 aatattatct tgataattta ttaaagagct atcccctaat atgtcaaaac ctgaattgtt
     3001 aaatgacgct atggcgtgaa aagcacttat accaatcgct tttggcaaag gaaataacgg
     3061 gataaaaacg gctagattaa taagaaaacc gataaattca acaatgagtg taaatatgac
     3121 aattttctta attagattaa caacacccgc catacttggt gagttaagat ttgtttgtaa
     3181 aagaacacga ttagaaatcc caattttcat cccaattaaa atcatcacaa acacacttaa
     3241 agtgatgatg ccaagcccac ctatttgtgt taatagtgca attacaactt taccaaatac
     3301 tgaaaatgta gtggataaat tatgaatggg tgacaaacct gttattgtaa ccgcagaagt
     3361 agaaataaag aaagtatcca aaaatgagag tgttactcct tcttgcttcg caaacggtaa
     3421 tgaaagtaac aaggcaccaa taataattac acttaaaaaa gaaagcacaa taattagata
     3481 tggtgacatt ttctttttaa tacctggttt taatttcttt tcttttttat tcatatttta
     3541 tattttatgc ttttattcat gaaagtgtta aagataacac taaataaaat atcgcccttc
     3601 tttgttactt aaggataaat ggtataattt aaatgttaat ttaataagaa gattggagtt
     3661 aatgatgaaa aaaagattaa tgaccttgcc acttattatg ttattactaa gtggtctaaa
     3721 atccccgata tcaaacattg aaaaacttaa tgcagcaaca ccgatgccga cgacgattgt
     3781 tttcgaaaat aacactgacg cggaagtaag tgcatattac actggtgttg aaggaaaaac
     3841 gggagacgaa ttacttgaat ttctctactc tgttattaaa gatcataatg agtatgacta
     3901 tgagaataat acgcatcgat atatttataa aatcattgat cgtaattggg aattagatgc
     3961 aattgatacc gtatcaccag cgaatttaga aaattttgac tatgtcaatg acaatggatt
     4021 cattcataaa ttatacgctg actataatga tgatattaat actgctgatc gctttaaaaa
     4081 tgaaggtgcg agtcgtgtta gttttgatag agaacatatt tggccgcaat ctttaggtgc
     4141 ctttggtcgc actggtgggg cgggaagtga cttccactca ctatggccaa gtgatgttaa
     4201 ggctaatcaa caagcgcata gtaataataa ttatggggtt ccaacctcta atattactaa
     4261 ttatcaaagt gaccacgata cttatgttgg ccgcaacgga aatgttgaag gttatacatc
     4321 aaaagtgttt gaaccacttg atgaatataa aggtgatatt gctcgcgcta tgttttatat
     4381 gccagcgcgt tattatgaat atattgataa actacatcca aaactagaac tagtaaacgg
     4441 ttctcctgct gcaagagaag ctagtgcgac actaaccggc caagctggtt gtcttgaaac
     4501 attactgcaa tggcacgaag aagatcctgt tagtgaatat gaaattagaa gaaacaactt
     4561 aatcgctaat aactatcaag gtaacagaaa tccgtttatt gattacccgc aatgggcacg
     4621 gattgcttat gatgaaggtt acattggcac aggagcgaca aacgcattag gactttctag
     4681 tgctgcgggt ttacgcgatc cttttgaaga tgttacctta aagagtattt caattgatac
     4741 gacaaatgtt aaaaaagaat atgcattaaa tgaaggttat gacccaacag gactagttgt
     4801 aaccgctcat tatgaaaatg gtggcaaacg aaaagttgca gatttcacta ctagtatggc
     4861 taaaggcgaa cctttaacca aaggtggaac aaaaaccata actgttagtg tcactcatga
     4921 aggggtaaca aagactgcaa gttataatat tgaagtcaaa gaaccactac caatctatgt
     4981 ttatatcggt gtagcagcag tagttctttt aataattatc atttatatct ttagcgcgaa
     5041 gtctaaaaaa gcaaagaaaa agcgtgcttc taatgttaaa aaagttatta aaaaagcgac
     5101 caaatcaagc tcttcaacga agagtaatag taaaagaaaa acaacaaaaa caaagaaaaa
     5161 ataactaata acatttatct taaattaggc gtattttacg acaaaacacg ccttttttat
     5221 tatttatgga cttttttaat gttagtgcta taatcattat tacaacataa ggtagatagt
     5281 tattatgaaa ttcaataaat taattagtgt ttcaatcctt gcactattaa taagcggaac
     5341 ttcgcttact acaactaaac aaaaagaaaa agtttcagcg gctaccccga tgccgacgaa
     5401 tattaatatg actaatagga ctgacgccga agttagtgct tattacgaag gggtgaacgg
     5461 taaaagtggt gatgcacttt tagcctttct ttatacgaaa attaaaaatc ataatgaata
     5521 caattatgaa agtgtaacgc atagaacaat ttataaaatt attgatcgga attgggcttt
     5581 agatgcactt gatccaaacg gccctgcgaa tacatctaat tttaactata gtactgataa
     5641 cggctttatt cgtaagttat acgctgatta taatgataat attgctactg cggatcggtt
     5701 caaaaacgac ggagcgagtc gtgttagttt tgataaagag catatttggg cgcaatcact
     5761 tggtaacttt ggtcgcactc gtggagcagg tagtgatttt cattcattat ggcctgctga
     5821 tgttaaaggt aatcaagatg cacatagcaa ctataatttt gcggttccaa aaacaagtat
     5881 tacaacttat aagaatgact acggcactta tgtcggtcgt aatggttata taagtggtta
     5941 ctcgcaaaaa gtatttgaac cgttagatca atataagggt gatatcgctc gtgctatgtt
     6001 ttatatgccc gcgcgttatt atgaatatat tgacgcactt catccgaaat tagaattagt
     6061 gaatggttca ccagcagcag tgaccgccag ttcgagtgtt actggtaaag caggcgattt
     6121 agcgactcta ctggaatggc atgaacttga tccagttgat gaatatgaaa ttagaagaaa
     6181 taacttaatt gcgaataact atcaaggcaa tcgcaatccc tttattgatt acccgcagtg
     6241 ggcacggatt gcttatgacc caacttatac cggtgcggga gcgactaatg ctcctgaaac
     6301 atctagcgtt ggcactaatc ctggtggtct tggtgaaacg gtactttcac atataacagt
     6361 tgatactaca gacgctaaaa caacttactt tttaggagat gattttacta caaatagcct
     6421 catagtaacc gcacatttta gtgatgagac atcactacca gtgcttgatt acacttcaag
     6481 cattgctaat ggtacagctt taaatacacc tggaaatcaa agtgttacta ttagttatac
     6541 acatgaagga gttactaaaa acacctcata cactattctt gttaaagagt ttgtcgtaag
     6601 tgattattta tttataagtg aagtatatgg tggtggtggt aacgctgggg cggaatataa
     6661 aactgacttt attgaattat ataattatgc tgattttccg ctatcacttg aagggtactc
     6721 tgtgcagtat gcttctgcag ctggaacaag ttggcaagtg actaatttaa gtggtacgat
     6781 tgcaccaaaa tcattttatt taattgaaga agctagtggt tctggtggaa cgagaaactt
     6841 accattagca gaaacagtcg gttctattcc catgcatgca aaatctttga aactggcgtt
     6901 agttaattca acgactgcgc tttcaggtgc ggatccaaca agtgatactt ctattattga
     6961 ttttgttggt gtcggaacgg ctaatgcata tcttggatta acagcagcac ctgaaattag
     7021 taatactatg tctgcccaaa gaaagtttat tattgaaggt attgctcctc cagttgaaaa
     7081 aagtaatgga acagatttta ttgctgcttt accaacacca acaaatgctg ctcttactat
     7141 tgctgttgat ataagggcag aaggcgatac tcctaatcaa tgtttaactt tatatagtcc
     7201 ttataaatca agagtgcttt cgcttagtcc gtcgcagctt aattacttta aaacagttgt
     7261 agcgggtgaa gaagaaagat taacagatgg tcgcgcccga tacgaagcgt gggcacttca
     7321 tttaggtgat gctttacctt atgaatacaa tgaaaacttt ggacttggaa acacgaacaa
     7381 gcaaacattt actagcgata ttgttatgct tgtccttgtc attgctctct ttggtttatc
     7441 aatcggattc tattttatta aatcaaaaca aaacacagca aaaacacatt aaaatttgca
     7501 aaaacagctt attttattta aaaaagaggg ccaaaacctc ttttgaaata tcttatgatt
     7561 ctatttgatt aaataacaaa aacagttatt ttattaaatc tagcttgcgc tgctaatgag
     7621 aatgtaagtg tgttacttgg agaaaataaa gtgaaaagta cattatcacc ttcggcaata
     7681 aagtcgactg tttctggagc ggaattagta accgctaaaa cagagtcacc cttctcccaa
     7741 gcgcccgcag tttctaataa cacagcatta atttgtactt cagcaggaac agtaaaagta
     7801 actacttgtc cagcgtaaat ccgcaacgga tttgtaaagt aacctacctc attttgggtt
     7861 gttaaatttc ctacatttac tgtcgatgca cctttatcaa tcgtcattgt gacaccactt
     7921 tctgttgtcc aaattgattg caaggcatcg cgttttgtta tttggtcttc agtagcaaaa
     7981 tcaatgactg atggaatacc ttctggggta actattgttc cgccgccgtt agttgcgagt
     8041 gcttcaataa aaataaacat accatcagga taatcagcgt caccagttac atagaaataa
     8101 tcaatttgaa tcgtggtttc actattaagt ccaatataat agtcatcttc ttcataatat
     8161 acaacttgat aatttactgc ttgtaaaata gcggtataat cttcagcttg atttgtgccg
     8221 ggtataaaca ctgttaaata gtgctcaaaa tttggatcat caagcgttaa ttcaacataa
     8281 cttcttgtta catttggtag cgctggtacc ttttcttcca cattatgact aagtaaatat
     8341 tcatcaatag cggctgatgg ccaatcgtta tatgtatcca tgccaattaa tccagcatat
     8401 tcacttgctg cttcgcttga atcttcgatg actacactag tttcggtgct aggagcgcta
     8461 ggttcttcac ttttagttat cgaagatgaa ccttctttac aaccagttaa tagtaaaact
     8521 aaaagcataa ctgtactaaa taatttattt ttcatagttt cttctccttt aataatagga
     8581 attatactaa tattgctttt tatttcaaaa taaaagtact ttaaatcaaa agaaaaatgg
     8641 ccgct
//
LOCUS       DAOSTA010000040         8188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_8277_length_8188_cov_9.154433, whole genome shotgun sequence.
ACCESSION   DAOSTA010000040 DAOSTA010000000
VERSION     DAOSTA010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8188
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8277_length_8188_cov_9.154433"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..570
                     /gene="spx"
                     /locus_tag="PLU08_04160"
     CDS             124..570
                     /gene="spx"
                     /locus_tag="PLU08_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009488107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HOQ70915.1"
                     /translation="MIKIYTSPSCSSCRKVKQWFDEQQIPYEEHNIFSASLSEAELKE
                     ILYKSENGTDDIISTRSKVIKEKNIDVNDLTMSELVQFIKDNPSVLKRPIMVDTNRIQ
                     VGYNSEEIMAFIPRERRAQMEAELMMGVRQQNEDTEICLDDDCPTK"
     gene            complement(586..1164)
                     /locus_tag="PLU08_04165"
     CDS             complement(586..1164)
                     /locus_tag="PLU08_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOQ70916.1"
                     /translation="MATSIEIEAKALLTKKDYNKVLKHFKLDVVDGYIQKNYYIDTEN
                     KDLRKLGLSLRVRRLNGYVISFKLPMAEGLLEKTQTLSREQFEDFETHGKFPEGDISD
                     FIESLYISPSDLKIIATLTTLRIEQEYEDLTLAIDENRYGELQDFELEMEATSMKKAQ
                     YLLKKVCEEVGIDYKENLVSKQKRAMDALSAV"
     gene            1220..2026
                     /locus_tag="PLU08_04170"
     CDS             1220..2026
                     /locus_tag="PLU08_04170"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003424.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOQ70917.1"
                     /translation="MALKINVIVREDEKSLSVSKSLCELALQKGFLIDEVNPDFVFSV
                     GGDGTFLKAVHRYLTKVKLIKFVGIHTGSLGFFADFRPEDINNLLDNIIKEELNTNAY
                     RLVKAEITQNNKKRNFYAVNEVRIENVHHTLVLDVYLNDEHLEMYRGNGVLVATQLGS
                     TGYNKSIGGAVINRNLELLEYTKIAPIANSAFRPLVNPLIIGEKDVLVFNGKNANTYF
                     GYDAFTKKLSDIPFSVKVSLSNKKITIVHRQNRPYSRILNESFLGGKHDI"
     gene            2016..2372
                     /locus_tag="PLU08_04175"
     CDS             2016..2372
                     /locus_tag="PLU08_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HOQ70918.1"
                     /translation="MTFSEFLQQYGVYLAFVVFLAIVFLIILILVIPRLPKKGTKSVE
                     PSISKLEIITLLGGEDNIKEIALRGSRLTVSLADKSLANIEELKKHGVDRVIVMQAKL
                     VLLVNKEVASLFDELA"
     gene            complement(2349..2891)
                     /locus_tag="PLU08_04180"
     CDS             complement(2349..2891)
                     /locus_tag="PLU08_04180"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOQ70919.1"
                     /translation="MFILASKSPRRQELFKLIIDDFAIIVPDIDENIPVSIKSDTAKI
                     ISKFKAYHIKAKYPEATILACDTIVVIDGELLGKPKDEDDARRMLTKLSGRKHHVISG
                     FTLVSPEFEINKNVVTEVYFNTLSPELIEAYIKSGSPFDKAGAYGIQDEEFKLVREIK
                     GSYYNVMGLPVEELRKFIKK"
     gene            complement(2893..4575)
                     /locus_tag="PLU08_04185"
     CDS             complement(2893..4575)
                     /locus_tag="PLU08_04185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOQ70920.1"
                     /translation="MAINSKFEMPVSIYALGGLGEVGKNMYVVEDEKNILLFDAGVLF
                     PEQDLPGIDYVIPDFAYLRDNARKIKALFITHGHEDHIGAIPFLVQVVPVPVIYAPPL
                     AAALIRHKLQMMRIKTPVNIVEYDGDTVVKFGSMTVSFFAVTHSIPDCYGFCVDTEHG
                     RVVGTGDFKIDLTPIGRQMDLGKIAELGNEGVDLFLSDSTNAEHEGFTPSERTVISAI
                     NEIFANTSGRLIISTFSSNISRIQQIVEATVNYRRKIIIIGRSMERVVSASRDLGYIK
                     IPDASIVDVGKSSTLKPEETVVLCTGSQGEPMAALSRIADGTHKDISIIPGDTVVFSS
                     SPIPGNSVGIDRVVNQLTRLGATVLTNSILRNIHSSGHPSQEELKLMLRLVRPKYFMP
                     VHGEYRMLKLHAELAAKVGVPLENSFVLKNGETLELRNGKVTRGRSFPVADIYIDGNS
                     TEGMSKAVLTDRRQLNQDGMVAVLIAVDPKKNELLVPVEIYSRGFINWSISNLLPDAE
                     KHINKVLKRHYAEKVTTFGEVKNIVKDELEPFFYDKTGRKPMIIPVIMSKGI"
     gene            complement(4661..5200)
                     /gene="def"
                     /locus_tag="PLU08_04190"
     CDS             complement(4661..5200)
                     /gene="def"
                     /locus_tag="PLU08_04190"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016936264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOQ70921.1"
                     /translation="MLKIFKDSDPLLRKRAEQVSLPLSDSDRELALAMLEHLKESQDP
                     LFAEEHNLRPGVGLAAPQVGESKQIIVVHIPRGKTERLTYVLANPIIISSSVRRAYLR
                     SGEGCLSVDEDHPGHVYRHYKIRVKAFDILNNEEVEINTFGYPAIVLQHEIDHLHGVL
                     FYDRINEENKVRYPDALSL"
     gene            5293..5667
                     /locus_tag="PLU08_04195"
     CDS             5293..5667
                     /locus_tag="PLU08_04195"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HOQ70922.1"
                     /translation="MNQKLYELALDLHQNLEEHPSVLLLKEYEKAMLKDSTVKALIEQ
                     YDALQEEINSLLESCDLENKAIKAKCSTLYKLKYVLDNEPLVKRYNKQYNLVNKMYKN
                     ITEELTNAVGIKKEIKCLLKDE"
     gene            5646..6188
                     /gene="rsmD"
                     /locus_tag="PLU08_04200"
     CDS             5646..6188
                     /gene="rsmD"
                     /locus_tag="PLU08_04200"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOQ70923.1"
                     /translation="MLAKGRVIAGKYRHRHLIALFDEKTRTTKDRVKEAIFSAVGDYI
                     NGKTVLDLFAGTGALGIEAISRGAAHGIFCENDSKTYDVLTGNLQMIEEPYNLYFEDY
                     KSALAKIKPNSVSIVIIDPPYRYDIDEIINNVFKAKILTNSYTLVLETDKEYAGVINN
                     AKIKKYKYGLTHVTIIRGEL"
     gene            6185..6661
                     /gene="coaD"
                     /locus_tag="PLU08_04205"
     CDS             6185..6661
                     /gene="coaD"
                     /locus_tag="PLU08_04205"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007086244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOQ70924.1"
                     /translation="MKLIAIYPGSFDPITNGHIDILNRALKIFDNVIVLVADNPAKKP
                     TFSVEERKAMIRESINGNPNVKVDSTTGLTIHYAEKVGAYALIRGLRAAIDFEYEFQI
                     NSANSFINSGVESVFLMAKQEFTFISSSTIKEMVSGGVDVSSLVPKPVLKRLEKLK"
     gene            complement(6662..7426)
                     /gene="pgeF"
                     /locus_tag="PLU08_04210"
     CDS             complement(6662..7426)
                     /gene="pgeF"
                     /locus_tag="PLU08_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOQ70925.1"
                     /translation="MYKWNDFKKITAVTSKRTGGFSTGDFSSLNLALHVGDDKETVFK
                     NRECFFGKKEVMMAPKNSVFVAQNHSTIVKKVTFKDAGRGFYDFNDGIKADALYTDEP
                     GLNLAIYHADCVPVFIYVPTHNIVGIIHAGEQGSVGNITGILVRKLFLEGVKPEEVYA
                     HIGPSASMGHRVITKERAFELAALGGDIIKAIKATEPQYFIDLPLLNTLQLRTLGVPL
                     SNITYSNICTYENNDEYYSYARNKVTGRNISFIRLN"
     gene            7522..8127
                     /locus_tag="PLU08_04215"
     CDS             7522..8127
                     /locus_tag="PLU08_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOQ70926.1"
                     /translation="MDSKNLYDFLIKVQSIAKIGLLYTTDPYAKKNYNEIGQMTLSLL
                     EDLQAVNLERNNYFKRDIYPTPNISVRAIIFNKEGQLLMVQEKEDGGFSLPGGWADLY
                     DSPTEAILKETTEEAGAKIKIMGIVAFLHRTPFKTPTSVPEYVLVFKAEFVEFIGEHD
                     HEILTVKWVDPYNLPPLSHKVTKHEVERMINAALHDKTIFD"
BASE COUNT         2707 a         1410 c         1468 g         2603 t
ORIGIN      
        1 aaacattata atgaagacaa gaaggatgta aatttaacct tttcctttaa cgaattcatt
       61 tcaggtattg cttttacata gtaaataagt tatattaata cgcggaacaa ggaggtcctg
      121 aatatgatta aaatttatac atcaccaagt tgttcgtcat gtcgtaaggt taagcaatgg
      181 tttgatgagc aacaaatccc atatgaagaa cataacatct tctctgcttc acttagtgaa
      241 gccgaactta aagaaatctt atataagtcg gaaaacggca ctgatgatat tatcagcact
      301 cgtagtaaag ttattaaaga aaaaaatatt gatgttaatg acttaacaat gagtgaatta
      361 gtgcaattta ttaaagataa tccgtccgta ttaaaaagac cgattatggt tgatactaat
      421 cggattcaag ttggctacaa tagtgaagaa atcatggcat ttatcccacg tgaaagacgc
      481 gcccaaatgg aagcggaatt aatgatgggt gtccgtcaac aaaatgaaga caccgaaata
      541 tgtcttgatg acgattgtcc tactaaataa ggtttattta ttttattaaa ctgcagaaag
      601 agcatccatt gctctttttt gtttgcttac aaggttttct ttataatcaa tcccaacttc
      661 ttcacatact tttttgagca gatattgggc ttttttcatc gatgttgctt ccatctcaag
      721 ttcaaagtct tgtagttcac cataacgatt ttcatcgatc gctaaagtta ggtcttcata
      781 ttcttgctca atgcgtaatg ttgttaaagt agcaataatc tttaaatcac ttggactaat
      841 atataaactt tcgataaagt cactaatatc gccttcaggg aatttaccat gagtttcaaa
      901 atcttcaaat tgttcacggc ttaatgtttg cgttttttca agtaatcctt ccgccatcgg
      961 tagtttaaaa gaaataacgt atccatttaa gcggcgaaca cggagactta atcctagttt
     1021 gcgcaaatct ttattttcag tatcaatata ataatttttc tgaatataac catcaacaac
     1081 atctagctta aagtgtttta atactttgtt ataatctttc ttcgtcagta gtgcttttgc
     1141 ttcaatttca atactcgtgg ccatagtaat tacctctttc tatcaatatg atacaatata
     1201 aacgaagatt tataaagtta tggcccttaa aattaatgta attgtgcgtg aagatgaaaa
     1261 gagtcttagt gtaagtaaaa gtttatgtga acttgcacta caaaaaggct ttttgattga
     1321 tgaagttaat cctgactttg tctttagtgt tgggggtgac ggaacatttt taaaagcagt
     1381 ccatcgctat ctcactaaag ttaaacttat taagtttgtt ggcattcata ctggttcact
     1441 tggattcttt gctgatttcc gtccggaaga tattaacaat cttttagata acattattaa
     1501 agaagaacta aacactaatg cttatcgctt agtaaaagca gaaattaccc aaaataataa
     1561 gaagcgtaac ttttatgcgg tcaacgaagt gcggattgaa aatgttcatc acacacttgt
     1621 gcttgatgtt tatttaaatg atgaacatct agaaatgtat cgtggtaatg gggtgttagt
     1681 tgctacgcag cttggcagta cgggttacaa taaatcaatt ggcggcgctg tgattaatcg
     1741 taatttagaa ttacttgaat acacaaaaat agcaccgatt gcaaacagtg catttcggcc
     1801 acttgttaac ccgcttatta ttggtgaaaa agatgtgtta gtgttcaacg ggaaaaatgc
     1861 caatacatat tttggttatg acgcttttac caagaaatta agtgacatac cttttagtgt
     1921 taaagtgagt cttagtaata aaaaaataac aatagttcat cgtcaaaacc gtccttattc
     1981 ccgtatatta aatgaatctt ttttaggagg taaacatgac atttagtgag tttttgcagc
     2041 aatatggtgt gtatttagct tttgttgtat ttttagcaat tgtcttttta atcatattaa
     2101 ttttagttat tccgcgttta cctaaaaagg gtacaaaaag tgttgaacca agcatttcaa
     2161 aattagaaat tattacactt ttaggtggtg aagataatat taaagaaata gcgttacgcg
     2221 gttcgcgttt aacagtatca cttgcggata agtcgcttgc aaacattgaa gaattaaaaa
     2281 aacacggtgt cgaccgtgta attgttatgc aagcaaaact tgttttactc gtgaataaag
     2341 aagtcgcatc actttttgat gaacttgcgt aattcttcta ccggcagccc cataacatta
     2401 tagtaactac ctttaatttc acggacaagc ttaaattctt cgtcttgaat accataggct
     2461 cccgctttat caaatggtga gccgcttttt atatatgctt caataagctc aggacttaaa
     2521 gtgttgaaat atacttcagt aacaacattt ttgtttattt caaattcggg actaacgagt
     2581 gtgaagccac tgataacatg gtgtttgcgc ccacttaatt tagtgagcat ccttctagcg
     2641 tcatcttcgt cttttggttt accaagtagt tcaccgtcaa tgaccacaat tgtatcgcac
     2701 gctaaaatag tagcttcagg gtactttgcc ttaatatgat aagccttaaa tttagaaatt
     2761 attttagcgg tgtcgctttt aatgctaacc ggaatatttt catcaatatc ggggacgata
     2821 attgcaaaat catcgataat taatttaaat aattcttggc ggcgcggtga ctttgacgct
     2881 aggatgaaca tactaaattc ctttactcat gatgacggga ataatcattg gtttacgacc
     2941 agttttgtca taaaagaatg gttcgagctc atccttaacg atgtttttaa cttcaccaaa
     3001 agttgttact ttttcagcat agtggcgctt taatactttg ttaatatgct tttcggcatc
     3061 tggtaataaa ttagaaatac tccaattaat aaagccgcga ctataaattt caacaggcac
     3121 taaaagttca tttttctttg gatcaacagc aataagcacg gcgaccatcc cgtcttgatt
     3181 taattgtcgt ctgtcagtta ggaccgcttt actcatgcct tcggtagaat taccatcaat
     3241 ataaatatcg gcgactggga aagaacgacc acgtgttact ttgccattac gtaattctaa
     3301 agtttcaccg ttttttagta cgaaagagtt ttcaagggga acaccgacct ttgctgcaag
     3361 ttcggcatgt agtttcagca tccgatattc accgtgaact ggcatgaaat attttggacg
     3421 cacaaggcgc aacattagtt ttaattcttc ttgcgaaggg tgaccagaac tatgaatatt
     3481 acgtaaaata ctgtttgtta aaactgtcgc tccaagtcta gttaattggt taacaacgcg
     3541 gtcaatcccg acgctattac cgggaattgg tgaactacta aatacgacag tatcacctgg
     3601 aataatacta atatctttat gggtaccatc ggcaatacgc gaaagtgctg ccatcggttc
     3661 accttgtgaa ccagtacaaa gcacgactgt ttcttctggt tttagtgttg atgacttacc
     3721 tacatcgaca atactagcgt caggaatttt aatatatcct aagtcgcgag aagcagaaac
     3781 aacgcgttcc attgaacgac caataataat gattttacgc ctataattaa ctgttgcttc
     3841 gacaatttgt tggatacgac tgatgttact tgagaatgta gaaataatta gtcgtccact
     3901 tgtgtttgca aagatttcat taatagcact gatgacggtt ctttcacttg gggtaaaacc
     3961 ttcatgttcg gcattcgtcg aatcacttaa aaagagatca acaccttcat taccaagttc
     4021 agcaatttta cctaaatcca tttggcgccc aattggtgtt aggtcaatct tgaagtcgcc
     4081 agtcccaaca acacggccat gttcagtatc aacacaaaat ccgtaacaat cagggataga
     4141 gtgtgtcacg gcaaagaaac taacagtcat actaccaaat tttactactg tatcgccatc
     4201 gtattcgaca atgttaacgg gtgtcttaat acgcatcatt tgtaatttat gacgaatgag
     4261 cgctgctgct agtgggggtg cataaataac aggtactggt acaacctgaa ctagaaacgg
     4321 gattgcccca atatggtctt cgtgaccgtg agtaataaaa agtgctttta ttttgcgtgc
     4381 attatcgcgt aaataggcaa aatcaggaat aacataatca ataccaggta aatcttgttc
     4441 ggggaataat acaccagcgt caaaaagtag gatgtttttt tcatcttcaa caacatacat
     4501 gtttttaccg acctcaccta aaccaccaag ggcataaata gaaaccggca tttcaaattt
     4561 tgaattaata gccataatta tctcctagaa ttattaaata tatagatcaa tatcttactt
     4621 attatgccaa attaataatt aaatgtaaat ttaaaagtct ctacaaagag agagcgtccg
     4681 gatatcgaac tttgttttcc tcattaatcc ggtcataaaa taagacacca tggagatgat
     4741 caatctcatg ttgcaatacg atagcaggat aaccaaaagt atttatttcg acttcttcgt
     4801 tgtttaagat atcaaaagct tttactctta ttttgtaatg acgatataca tgaccaggat
     4861 ggtcttcgtc cactgataaa cagccttcac cgctgcgtaa atatgcacgg cgcacagaag
     4921 aagatataat aatcggattt gcgagaacat atgttaatct ttcagtttta ccacggggga
     4981 tatgaacgac aataatttgt ttactctccc caacttgtgg agcagctaaa cctactcctg
     5041 gtcgtaagtt atgttcctca gcaaaaagcg ggtcttggct ttcctttaaa tgctcaagca
     5101 ttgcaagggc gagttctcga tcagaatctg aaagtgggag cgaaacttgt tcggctcttt
     5161 tgcgtaataa gggatcacta tctttaaata ttttcagcat tcttctaact ccggaaatat
     5221 tttaacacat ctgtgttaac aagtgagtat taacgctata tgataattca tcatttcttg
     5281 tataattagt ttatgaacca aaaactatat gaattagcgc ttgatttaca tcagaatctt
     5341 gaagagcatc caagtgtttt acttttgaaa gaatatgaaa aagcaatgct aaaagatagc
     5401 acagttaagg cactgattga gcaatatgac gccttgcaag aagagattaa tagtttgctt
     5461 gagtcgtgtg atttagaaaa taaagcgatt aaagcaaaat gcagtactct ttataaatta
     5521 aagtatgttt tagataatga accactggta aaacgctaca acaagcaata taatcttgtt
     5581 aataaaatgt ataaaaatat cacagaagaa ctgacaaatg cagtgggaat aaaaaaggag
     5641 attaaatgct tgctaaagga cgagtaattg ctggaaagta tcgacatcgc caccttattg
     5701 ccctttttga cgagaaaaca cgaacaacaa aagaccgtgt gaaagaagca attttttcag
     5761 cagtgggtga ttatataaac ggcaagacag ttcttgattt atttgccggt actggggcac
     5821 ttggtattga agcaatctcc cgtggcgctg ctcatggtat tttctgtgaa aatgatagta
     5881 aaacatatga tgtattaact ggaaacttac aaatgattga agagccttat aatttatatt
     5941 ttgaagatta taagagtgct ttagcgaaga ttaagcctaa ttccgtttct attgttatta
     6001 ttgacccacc ctaccgttat gacattgacg aaattattaa taatgttttc aaagctaaaa
     6061 tattaacaaa tagttacact cttgtattag aaacagataa agaatatgca ggagtgataa
     6121 ataatgctaa aataaaaaag tataaatatg ggttgacaca cgttaccata attagaggtg
     6181 aattatgaaa ttaatagcta tatatccggg gagttttgat ccgattacaa atgggcatat
     6241 tgatattttg aatcgagcgt taaaaatatt tgataatgtc attgttctag ttgcagacaa
     6301 tcccgctaaa aagccaacat tttcggtgga agaacgaaaa gcgatgatta gagaatcaat
     6361 aaatggtaat ccaaatgtta aagttgattc aacaactggt ttaactatac attatgcaga
     6421 aaaagtaggg gcatacgccc tgattcgtgg gttacgcgcc gccatagact ttgaatatga
     6481 gttccaaatc aatagtgcaa atagttttat taattctggc gtggaatcag tctttttaat
     6541 ggctaaacaa gaatttacat ttatttctag tagcacaatt aaagagatgg ttagcggagg
     6601 cgttgatgta agtagcctcg tacctaaacc agttttaaag cgattagaaa aattaaaata
     6661 attagtttaa acgaataaaa gaaatattac gacctgttac tttattcctt gcataggagt
     6721 agtactcgtc gttattttca tatgtacaaa tattgctgta agtaatatta ctaagtggta
     6781 caccaagcgt gcgaagctgc aaggtattaa gtagtggtag atcaatgaaa tattgaggtt
     6841 cagttgcctt tattgccttg atgatatcac cacctaacgc agcaagttca aaggcacgct
     6901 cttttgtaat tacacgatga cccatgcttg cgcttggtcc aatgtgtgca taaacttctt
     6961 caggtttaac accttcaagg aataatttac gaacgagaat accagtaata ttgccgacac
     7021 ttccttgttc accagcgtga ataatcccta cgatgttatg tgttggcaca taaataaaaa
     7081 ctggtacaca atcagcgtga taaatagcaa gattaagtcc aggttcatca gtatagagag
     7141 catcagcttt aattccatca ttaaaatcat aaaaaccgcg tccagcatct ttaaaagtta
     7201 cctttttaac gatcgtacta tgattttgag caacaaacac actattttta ggtgccatca
     7261 tcacttcttt tttgccgaaa aagcattctc tatttttaaa aactgtttct ttatcatcac
     7321 caacgtgtag tgctagattt aatgaagaaa aatctccggt tgaaaacccg ccagtccgtt
     7381 ttgaagtaac agctgtaatc tttttaaaat cattccattt atacataagg cgcctcctat
     7441 tcttattata cataaaaagt agttaaatta ttattcatag taagccatca ctttaaacta
     7501 gtatgttata ctaaatttat tatggatagt aaaaaccttt atgattttct tattaaagta
     7561 caatcaatcg ctaagattgg attattatat acaaccgacc cgtacgcaaa gaaaaattat
     7621 aatgaaattg gacaaatgac gctttcatta cttgaagatc tgcaagcagt gaatttagag
     7681 cgaaataatt atttcaaaag agatatttat ccaacaccaa atattagcgt tcgcgctatc
     7741 atttttaata aagaaggcca attattaatg gttcaagaaa aggaagacgg gggtttttcg
     7801 ttgcctggag gatgggcaga tttatatgat agcccaactg aagccatctt aaaagaaaca
     7861 actgaagaag ctggagcaaa aattaaaatt atggggattg tggccttttt gcatcgcaca
     7921 ccttttaaaa ctcctacctc tgtgccggaa tatgttttag tgtttaaagc tgagtttgtt
     7981 gagtttattg gggaacatga ccatgaaata cttaccgtaa aatgggttga tccttataac
     8041 ttgccgccac tttcacataa agttacaaaa catgaagttg aacgaatgat caatgccgcg
     8101 cttcatgaca aaactatttt tgattagcat atactcgtac cacgtggagc gcataaatgc
     8161 tataataaaa aataaaatta aaggagat
//
LOCUS       DAOSTA010000041         7896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_8655_length_7896_cov_7.735748, whole genome shotgun sequence.
ACCESSION   DAOSTA010000041 DAOSTA010000000
VERSION     DAOSTA010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7896
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8655_length_7896_cov_7.735748"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1250
                     /gene="pilM"
                     /locus_tag="PLU08_04220"
     CDS             66..1250
                     /gene="pilM"
                     /locus_tag="PLU08_04220"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOQ70927.1"
                     /translation="MDIKISTLEISASAVKLVVGYELEGSPYVLYANKVALSQAISAG
                     EIVAPDQVINAIKVLAADAREALGETISEVVLALPTIDFEVFRGTQTTNTLSNKIDRI
                     DIKNIMALFRKNRVDPNKVIVAILPQVFKTDSDQVYRLLPLGEVSNTLTVEAFLHVLP
                     IWIYNSYIETVQKAGLKVRKVYPDKYALTELLNSPATATNTYFLVDFGAKQTSITFVS
                     NHNLYFSRIINEGSDDLTYLLAQKYGLELKEADRLKKDFGLDHRNVSVNFPLATGIGE
                     RGEEIKVFLSDIYSTCQTFLNSHLQKVLSEIKIVGKSQNMPNLNKYPLLFVGGGSTIY
                     NYEEIIGSDRSFPDKYIVKLDTIGARDLTYAVNLGLIKAYSKYAQDIGDEKASIGTLT
                     RS"
     gene            1253..2347
                     /gene="ftsZ"
                     /locus_tag="PLU08_04225"
     CDS             1253..2347
                     /gene="ftsZ"
                     /locus_tag="PLU08_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOQ70928.1"
                     /translation="MIGTNDLDNFEPVARIVVIGVGGAGNNAVNRMIDDQIDSVSFWV
                     ANTDKQALATSKAKNRIVLGEEFTHGLGAGGEPSVGRKAAEASRPEIEKIVEGADLVF
                     IAAGMGGGTGTGASPIIAEIAKAAGALTIAIVTRPFTFEGKKRIANSVEGLNALKEIV
                     DSIIVVSNDKLLITGGNLPIAQAFSESDKVLAQSVKTVVDLILVPAVINLDFADVRNT
                     LLNSGIALIGFGMGKGKNKAHDAAQTAINSPLLEASLQGARKAIIAVTCGTNVSLFEA
                     QETVNEIVETSGSNLDVKFGVALNELLEDSIIVSVIASDFEEQYDFTSTHTSIIKPVT
                     PESRTYHAEDDEDDDSFLPNILKELEAEEN"
     gene            2618..5335
                     /gene="ileS"
                     /locus_tag="PLU08_04230"
     CDS             2618..5335
                     /gene="ileS"
                     /locus_tag="PLU08_04230"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009330839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOQ70929.1"
                     /translation="MELKDTLLMPKTKFEMRGNLTRKEPGILARWEAIDLYKLMVKKN
                     ETSKPFILHDGPPYANGNIHSGHMLNRNLKDFVVRMKNMQGYDTPFIFGWDTHGLPIE
                     NQVTKSGVNRKAIPITEFRKLCKEYALKQVEIQKAQLKRLGLVGDFENAYLTLTKDYE
                     ADELRVFKRMALKGLIYRGLKPVQWSPSSETALAEAEIEYKDVVSDSIYVLFNVTKGN
                     DKVPVGTKFMIWTTTPWTLPANLAISVHPAFTYGVYESALGDVILLPELVEEVSKASD
                     LTFTLKYEVKGADLDLIETKHPLYERISLVITGMHVTSESGTGAVHTAPGHGLEDFVV
                     GQKYGLDVLCPVDARGYFTSEAGKYEGLFYEDANKVIINDLREVGALVAHTKVKHSYP
                     HDWRTDKPLIFRATPQWFASIEPIRRDLLNAVQKVDWYPAWGEGRISNMISDRGDWTI
                     SRQRVWGVPIPIIYNEDGSPILEEVVFDNIIAEVEKHGSNIWYEKDAKYFLPEGYQNA
                     LSPNGNFTKEVDTMDVWFDSGISAIHTLKKHGLPFPADLYLEGNDQYRGWFNSSLTLS
                     VAALEKAPYKAVVTHGFVVDDKGEKMSKSKGNVLDPSKLANEYGADILRLWSATIDYQ
                     EDARLSVDLLRQTSETYRKIRNTFRFMLGNLPALDTSFAELKALYKEPDFYLDKLMTN
                     KLQQVINKMIDSYNDYDFGAAFTTLMNFVTNELSSFYLDVAKDILYCEAKDSARRQSV
                     VGTLYKITVALLPLIAPVLVFTAEEIYEHLPVIKRLPSVHLMDFPYQLDVDNAKLEEF
                     SALLKVRSNAYQHIEKARQEGVLGSSQEASLTLPHSEVEVAPQLKKLDKEELARFFIV
                     SEVKFCNTYKVEKHTGHKCPRCWNYHDELVSVAEEQVCERCAKVLNTYE"
     gene            5328..5891
                     /gene="lspA"
                     /locus_tag="PLU08_04235"
     CDS             5328..5891
                     /gene="lspA"
                     /locus_tag="PLU08_04235"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOQ70930.1"
                     /translation="MNKVKQYLKSFFYSYAWLAVLLLIIDQLTKVWALKAKLDITLIP
                     NFLHLTYVRNTGAAWSMFDGNMLALALISFVVGTLLIVARIKYRTKLDTFKKILFAVV
                     LAGTWGNFIDRAFYKLLTGAEGVVDYIHFQFGNYHFPVFNVADICITLGILSYVVLLL
                     VEEFKALKAPKTEEKVKDDTNEEKDGE"
     gene            5881..6795
                     /locus_tag="PLU08_04240"
     CDS             5881..6795
                     /locus_tag="PLU08_04240"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOQ70931.1"
                     /translation="MENKLLKVDFEHSGKRADVVLSSLLSITRAQVQKMIKDGDIKLN
                     GTSVKASYRIEGGEEFSVTIREKEAPNLTPYEIELDVRYEDEYLLVVNKPAGLVVHPS
                     FGHKKETLVHALLARGDKYSDVNGVFRPGIVHRLDKETSGLLIVCKNNEVHNKIAKLL
                     KKREITRRYTALVRGKVREEAGVIKTYLTRSKDNFKKMANTPATGKLAISHFKVKERF
                     KGYMLLEVELETGRTHQIRAHLEFINTPIVGDALYGLKKDPLYSKGQLLHASYLAFTH
                     PYTGELVEVSAELPDYFTKVLRRLQKDL"
     gene            complement(6758..7690)
                     /locus_tag="PLU08_04245"
     CDS             complement(6758..7690)
                     /locus_tag="PLU08_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HOQ70932.1"
                     /translation="MHNYELRIPREDLGIVIEQTKLPSVSNKHSYMIIYYEKGNIKFS
                     IYDNTKNEPYKARFVGARLPKYLEPYLKEKDIKTPKSKSVTTKIVDKKITTFPHIGSD
                     EVGFGDFFGPLVVVAAYLDEELKALVESLNVKDSKALDDTKIVLLGNLLKDKIPHTKN
                     IVHNPKYNELIAQGYNMNQMKAMLHQNVLTTLAKRQKYDGKLYLDAFTSDKHYENYTA
                     LMSKAKVIQLKDGESNSLAIATASILARFYFLREIEVLNIRYNTKIPLGAGNAVDKFA
                     QEFLVKFGKNNLLSVVKQNFRNFKDLFADDGGLL"
BASE COUNT         2540 a         1330 c         1629 g         2397 t
ORIGIN      
        1 tattaattag ttgcaattgt tattattcgt taattattgt aaaataatta cattattgga
       61 gaattatgga tattaaaatt agtacattag aaattagtgc cagtgccgtc aaactcgttg
      121 tcggttatga acttgaaggt agtccctatg tgctttatgc aaataaagtg gcgctttcac
      181 aagcaataag tgcgggagaa atagtcgcgc ctgaccaagt cattaacgcg attaaagttt
      241 tagcagcgga cgctcgtgaa gcattaggcg aaacgattag tgaagtagtg ctagccttac
      301 caacgattga ttttgaagtg tttagaggaa cacaaacgac aaacacgctt tcgaataaaa
      361 ttgatcggat tgatattaaa aatatcatgg cacttttccg taaaaaccgt gttgatccaa
      421 ataaagtaat tgtggcgatt ttaccgcaag tttttaaaac agatagcgat caagtgtatc
      481 gcttactccc acttggtgaa gtaagtaata cgctaacggt agaagctttc ttacatgttt
      541 taccgatttg gatttataat tcctatattg aaacagtgca aaaagctgga cttaaagtaa
      601 gaaaagttta tcctgataaa tatgccttaa ctgaactttt aaatagtcct gcaacggcca
      661 ctaatacgta tttcttagtt gactttggcg caaaacaaac ttctattact tttgtctcta
      721 atcataactt atatttctca cgcattatta atgaaggaag tgatgactta acttaccttt
      781 tagcgcagaa gtatgggctt gagctaaaag aagccgatcg gcttaaaaaa gactttggtc
      841 ttgatcatcg taatgttagt gttaatttcc cacttgcgac cggaattggt gaacgcggtg
      901 aagaaattaa agtgttttta agtgatattt attcgacatg tcaaacattt ttaaatagtc
      961 acttacaaaa ggtattaagt gaaattaaaa ttgtgggaaa aagtcaaaat atgccaaact
     1021 taaataaata tccactgctt tttgttggtg gtggtagtac tatttataat tatgaagaaa
     1081 ttattgggag tgaccgttca ttccctgata aatatattgt taaactcgat accattgggg
     1141 caagagactt aacttacgct gttaatttag gactcattaa agcttatagt aagtatgccc
     1201 aagatatcgg ggacgagaaa gcaagtattg ggacactaac aaggagttaa ctatgattgg
     1261 aacgaatgat ttagacaatt ttgaacctgt agctagaata gtagttattg gtgtcggtgg
     1321 cgctggtaat aatgccgtaa atcgcatgat cgatgatcaa atcgatagcg tatctttctg
     1381 ggtggcaaat accgataaac aagcacttgc cacaagtaaa gcgaaaaatc gtattgtgct
     1441 tggtgaagaa tttacacacg gtttaggtgc tggtggtgaa ccaagtgttg ggcggaaagc
     1501 cgccgaagct agtcgtcccg aaattgaaaa aatcgttgaa ggtgctgacc ttgtctttat
     1561 cgctgcaggt atgggtggtg gaacaggtac aggtgcctcc cccatcattg ctgaaattgc
     1621 gaaagccgct ggtgctttaa caatcgcgat tgttacgcgg ccctttacct ttgaaggtaa
     1681 aaaacgtatt gccaatagtg ttgaaggact taatgcctta aaagaaatcg ttgactccat
     1741 tatcgttgtt agtaatgata aattacttat cactggtgga aatctcccaa ttgcccaagc
     1801 ttttagtgaa agtgataaag tattagcgca aagtgtaaaa acagttgtcg acttaatttt
     1861 agtaccggct gtgattaatc ttgactttgc cgatgttcgt aataccctct taaatagcgg
     1921 tattgccctt attggctttg ggatggggaa agggaaaaat aaagcgcacg acgcagcaca
     1981 aacagcaatt aatagtccgc ttttagaagc tagtctccaa ggggcaagaa aagcaattat
     2041 tgctgtcaca tgcgggacga atgtttcact ttttgaagca caagaaacag ttaatgaaat
     2101 cgttgaaaca agcggatcaa atcttgatgt taaatttggt gtcgcattaa atgaattact
     2161 tgaagactcc attatcgtca gtgtcattgc tagtgacttt gaagagcaat atgactttac
     2221 tagtacgcac acttcaatta ttaaaccagt gacaccagaa tcgcgtactt atcacgctga
     2281 agacgatgaa gatgatgata gcttcttacc aaatatctta aaagagttag aagcagaaga
     2341 aaattaatga cattattata ctttaatgta taatagtagc gaaatcggac gaagacacgg
     2401 tggcggtaga accttacaaa gcgactaggg aaatggtgca agccctaaga agaagctact
     2461 cccattatca cttctgagtg caactatgaa caagagtaat agttgtcgct taatctcacg
     2521 ttacgagata caaagtgcta gattagtcta gaacacaggt ggtaccgcgg agtaattcgt
     2581 cctgtgacta gacttttttt attgaaggag agagactatg gaacttaaag acacattatt
     2641 aatgccaaag acaaagtttg aaatgcgggg taacttaacc cgcaaggaac caggtatttt
     2701 agcgcgttgg gaggcaatcg atttatataa attaatggtg aagaaaaatg aaacaagtaa
     2761 accttttatt ctccatgacg ggccacctta cgctaacggg aacatccata gcggacatat
     2821 gcttaaccgt aacttaaaag actttgtcgt acgcatgaaa aatatgcaag gctatgacac
     2881 gccctttatc tttggctggg atacgcacgg tctcccaatt gaaaaccaag tcacaaaaag
     2941 tggggttaac cgtaaagcaa ttccaattac agaattccgc aagttatgta aagaatatgc
     3001 acttaagcaa gtcgaaattc aaaaagctca acttaagcgt cttggcttag tcggtgactt
     3061 tgaaaatgct tatttaactt tgactaaaga ttacgaggct gatgaacttc gtgtctttaa
     3121 gcgtatggca cttaaaggtt taatttatcg cggactaaag cccgtgcaat ggtcaccaag
     3181 tagtgaaact gcacttgctg aagccgaaat tgaatataaa gatgtcgtta gtgactcaat
     3241 ttatgtgctt tttaatgtaa caaaaggcaa tgataaagta cctgttggca ctaaatttat
     3301 gatttggaca acaaccccgt ggacgcttcc tgctaactta gcgattagtg tccatcccgc
     3361 ctttacatat ggggtttacg aaagcgcgtt aggtgacgtt attttactac ctgaacttgt
     3421 cgaagaagta agtaaagcga gtgacttaac ttttacatta aaatatgaag tcaaaggtgc
     3481 ggatttagat ttaattgaaa cgaaacaccc gctttatgag cgtatttccc ttgttatcac
     3541 cgggatgcat gtcactagtg aaagcgggac tggggcggtt cacacggcgc cgggtcacgg
     3601 gcttgaagac tttgtggttg ggcaaaagta cggcttagat gtgttatgtc ctgttgacgc
     3661 tcgaggatac tttactagtg aagctgggaa atatgaagga ctattttacg aagacgctaa
     3721 taaagttatt attaatgact tacgggaagt aggagcgctt gtcgcccata cgaaagtaaa
     3781 acacagttac ccacacgact ggcggacgga taaaccgctt atctttcgcg caacaccgca
     3841 atggtttgcc agtattgaac caattcgcag agatttactt aacgctgtcc aaaaagttga
     3901 ctggtatcca gcgtggggtg aaggccggat tagtaatatg attagtgatc gcggtgactg
     3961 gacaattagt cgtcaacgcg tatggggtgt ccccattccg attatttata atgaagatgg
     4021 tagtccgatt cttgaagaag tagtatttga taacattatt gcggaagtgg aaaagcatgg
     4081 cagtaatata tggtacgaaa aagatgcgaa atatttctta ccagaagggt accaaaacgc
     4141 attatcacca aacggtaact ttacgaaaga agttgacacg atggatgtgt ggtttgatag
     4201 cggcatcagt gcgattcata cacttaaaaa acacggttta ccttttcccg ctgatttata
     4261 tttagaaggt aatgaccaat atcgtggttg gtttaatagt tccttaactt taagcgttgc
     4321 ggccttagaa aaagcaccat acaaagccgt agtgacacat ggctttgttg ttgatgataa
     4381 aggcgaaaag atgtctaaat ccaaaggtaa tgtgcttgat ccaagtaaat tagcaaatga
     4441 atatggtgcg gatattttac gcttatggag tgcgacgatt gattatcaag aagatgcacg
     4501 gctcagtgtt gatcttttac gccaaacaag tgaaacttat cgtaaaatcc gtaatacttt
     4561 ccgctttatg cttggtaatt tacctgcgtt agacacaagt tttgctgaac ttaaagcatt
     4621 atataaagaa ccagactttt atcttgataa attaatgact aataaattac aacaagtcat
     4681 taataaaatg attgatagtt ataatgatta cgactttggg gcagctttta ctacactgat
     4741 gaattttgtt acgaatgaat taagtagttt ctatcttgat gtggcgaaag atatcttata
     4801 ctgcgaagct aaagatagcg ctcgacgcca aagtgttgtg ggaacacttt ataaaattac
     4861 ggtagcactt ttaccactga ttgctcctgt attagtgttt accgctgaag aaatttatga
     4921 gcacttacca gtgattaagc gtttaccttc cgtacattta atggatttcc cctatcaact
     4981 tgatgtcgat aacgctaaac ttgaagaatt tagtgcgtta ctaaaagtac gaagcaacgc
     5041 ctatcaacat attgaaaaag cgcgccaaga aggagtactt ggcagtagtc aagaagcaag
     5101 cttaacatta ccacatagtg aagtagaagt agcgccacag ttaaaaaaat tagacaaaga
     5161 agaacttgca cgcttcttta ttgtaagtga agttaagttt tgtaatactt ataaagtaga
     5221 aaagcacact ggtcacaaat gtccgcggtg ctggaattat catgatgaat tagtaagcgt
     5281 tgctgaagaa caagtctgtg aacgatgcgc aaaggtatta aacacatatg aataaagtaa
     5341 aacagtactt aaaatcattc ttttattcct atgcttggtt agccgtgctc cttttaatca
     5401 ttgaccaatt aacaaaagta tgggcactaa aggcgaagtt agacatcacg cttattccta
     5461 atttcttaca tttaacatat gtgcgaaata caggcgcagc gtggagtatg tttgatggta
     5521 acatgctcgc tttagcactg attagttttg ttgtcggtac gctgcttatt gtagcgcgca
     5581 ttaagtatcg cacaaagtta gatacattta agaaaatact ttttgcggtt gtccttgccg
     5641 gaacttgggg taattttatt gaccgcgctt tctataaact tttaacaggt gcagaaggtg
     5701 ttgttgatta tattcatttc cagtttggta attatcattt ccctgtgttt aatgtcgctg
     5761 atatttgtat tactttaggg atcttaagtt atgtcgtttt attattagtg gaagagttta
     5821 aagcgctaaa agctccaaaa actgaagaaa aagttaaaga cgacactaat gaggaaaaag
     5881 atggagaata aacttttaaa agttgatttt gagcatagtg gtaagcgcgc tgatgtagtg
     5941 ctttcttctt tattaagtat tacgcgcgcg caagtgcaga aaatgattaa agacggggac
     6001 attaaactta acggcaccag cgttaaagca agttaccgta ttgaaggcgg cgaagagttt
     6061 agtgttacta ttcgcgaaaa agaagcacca aacttaacac cctacgaaat tgaacttgat
     6121 gtgcgttatg aagacgaata tttgctcgtt gtcaataaac ccgcgggatt agtcgtgcat
     6181 cctagttttg gtcataaaaa agaaacttta gtgcatgccc ttttagcgcg cggtgacaaa
     6241 tatagtgatg ttaacggtgt ttttcgtcct ggtattgttc atcgccttga taaagaaaca
     6301 agcggtcttc ttatcgtatg taaaaataat gaagtgcata ataaaattgc taaactctta
     6361 aagaagcgtg aaatcactag acgatacaca gcgcttgtgc gcggtaaagt gcgtgaagaa
     6421 gcgggtgtaa taaagacata cttaacacgc agtaaagaca attttaaaaa gatggctaat
     6481 actcccgcaa ctggtaaact agcgattagt cactttaaag ttaaagaacg ctttaagggc
     6541 tatatgcttt tagaagtgga attagaaaca ggccgtactc atcaaattag agcgcactta
     6601 gaatttatta atacgccaat tgttggtgac gcgctttacg gtttaaaaaa agacccgcta
     6661 tatagtaagg gtcaattatt acacgctagt tatttagcgt ttacgcatcc gtataccgga
     6721 gaattagtag aagtaagtgc agagcttcca gattatttta caaaagtcct ccgtcgtctg
     6781 caaaaagatc tttaaaatta cggaagtttt gtttcacgac acttaataaa ttatttttac
     6841 caaatttaac caaaaattct tgcgcaaatt tatcaacagc atttcccgct ccaagcggga
     6901 tttttgtatt ataacgaatg tttaatactt caatttcacg aaggaagtag aagcgcgcta
     6961 atatactagc agtagcaatc gctaatgaat tactttctcc gtcttttagt tggatgactt
     7021 ttgctttact catcagtgcc gtatagtttt cgtaatgctt atcactcgta aaggcgtcaa
     7081 gatacagttt accatcgtat ttttgccgtt tagcaagtgt cgttaacaca ttttgatgga
     7141 gcattgcttt catttggttc atattgtaac cttgtgcgat aagttcatta tatttcgggt
     7201 tatgcacaat atttttagtg tgggggattt tatcttttag taaattacca agtaagacaa
     7261 tttttgtatc atcaagcgct tttgaatctt tcacattaag actttccact agggctttta
     7321 attcttcatc tagataagcg gcaacaacga caagtggacc aaagaagtca ccaaacccga
     7381 cttcgtcact gccaatatgc gggaaagttg ttatcttttt atcgactatt ttagtcgtaa
     7441 cgcttttact tttaggcgtt ttaatatctt tttcttttaa gtacggttca agatatttag
     7501 gtaaacgcgc accaacaaaa cgcgctttat atggttcatt tttcgtatta tcataaattg
     7561 aaaacttaat gtttcctttt tcataataaa taatcatata tgaatgttta tttgaaacac
     7621 taggtaattt tgtttgttca atcacaatac ctaaatcttc acgcggaatt cgtaattcat
     7681 agttatgcat gagtatattt taacataaac gacaatcttg ttcgttatta gaaaataatc
     7741 attaatgata aaatacaaat atggatatat ttacactttt agaatttaat gaaatcgtta
     7801 gtaaagttaa agatttaaca aagacagaat taggtggtga gtcccgtctt tagggttata
     7861 cactagaaaa gtttagggga taaaaataat aattaa
//
LOCUS       DAOSTA010000042         7539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_9148_length_7539_cov_11.395510, whole genome shotgun sequence.
ACCESSION   DAOSTA010000042 DAOSTA010000000
VERSION     DAOSTA010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9148_length_7539_cov_11.395510"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..323)
                     /locus_tag="PLU08_04250"
     CDS             complement(102..323)
                     /locus_tag="PLU08_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ70933.1"
                     /translation="MEINEAFGIRLRQLRKAKGWSLLELSVRANVNHNYLCDLENGRR
                     NPTLALLYRISEAFGITLSSLLLGIELIQ"
     gene            complement(310..1287)
                     /gene="whiA"
                     /locus_tag="PLU08_04255"
     CDS             complement(310..1287)
                     /gene="whiA"
                     /locus_tag="PLU08_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOQ70934.1"
                     /translation="MKNVSFTTRAKEDITFNTISYSLPRRKATLAALIKHAGHLIIRE
                     KKEVLKLSTENAKVAKYIYQLFKDIFNFEPTFSYVTKRKFGKKMKFNVIVEQKVDEII
                     ETLAVDFYDSAVSRKIVYSDDTLGGYFTGAFLGSGSMTSPLSSNYHLEIVVRQKKYAE
                     HLARMLNRFEKTNFNAKVTARRDKYIVYLKRSDQISSFLVFVGAANASLEFEGIRMDR
                     DMQNITNRMFICDEANYNKTVKSATEQIENIKIVQRVYGHTPHNNEKLGALMRLRLEN
                     EDATMNELAEMLTEELGSEKRITKSNVNHLFIKLRELAEPFKELSRGNK"
     gene            complement(1347..2270)
                     /gene="trxB"
                     /locus_tag="PLU08_04260"
     CDS             complement(1347..2270)
                     /gene="trxB"
                     /locus_tag="PLU08_04260"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOQ70935.1"
                     /translation="MNKEIIPILIIGAGPAGMTAAIYLSRANATFKIVDKYAPGGKMN
                     ITQDVENYPGIETISGPDLAFKMFEQLRSLGIEVGYADVNKITKEDDYFVIHSDEETL
                     YAKAVIVATGTQERKLKIPGEARYTGKGVSYCAICDGNLYKNDAIMIIGAGNSAIEEA
                     LFVSKIVKHVHIVHRNERFRADESLVNELKAMPNVTFHLNYIPVEILGDTKGVTSVAI
                     KNVIDGTDKVIEVKAVFPFVGSDASADFLSEFDVRDDKGFVLVNEEMMTSVPGLFSIG
                     DVNQKVLRQIVTATNDGAIAALAANRFVKRK"
     gene            complement(2263..2910)
                     /locus_tag="PLU08_04265"
     CDS             complement(2263..2910)
                     /locus_tag="PLU08_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOQ70936.1"
                     /translation="MKSKYDLVVLDLDGTIADSDLGLVKIGIKMSETYLPRKDINMDD
                     YLFLNGPTLDETMPYLFPGYPLEELIAKYNEYLPDSVKDITLFKGAKELIKTLKNNNI
                     ELALLTNRARASTEQILKRHGVHSYFKTLVCVDDGFKKKPSGEGLVHIMKTLKSAPDK
                     TLFVGDNWRDILAGQDAGVDVAFIHQHRRPHRLDVSATYVLDDISDVLGVVQSDE"
     gene            complement(2900..4210)
                     /gene="lgt"
                     /locus_tag="PLU08_04270"
     CDS             complement(2900..4210)
                     /gene="lgt"
                     /locus_tag="PLU08_04270"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOQ70937.1"
                     /translation="MDLTLVVVVIFGLIFIGAVTVLGLSLNEFVKKEEDINTLFKGKH
                     RLIAISVLSGAVSVLMLFLPLMVLSNSVLHSLLIGLGSFLFALMLLTFIAAFVLHYYK
                     FNVLQREWIKESKIVTIISGVLSIVFLFVLLEGLTLAEIIKFPLPRGIPFGDSPVIAF
                     YAIFILTGALLVLAITDHEFYKKYGRHGILENVFYVAFPAGIIGARIWYVIGEWNNPE
                     SGFAENPLTIFAIRDGGLAIMGGALFGIIAGVWFFVKRRKAYDIGFAADIIIPTILVA
                     QAIGRWGNFFNQEVYGGDITDISKWWFLPEFIKRQMFILGKYRQPFFLIESALNLTGY
                     FVIRYGVGEGLKKYRKPFDMAFMYIVWYGLVRFIMEPLRNPMFRMGAGGKWSEYNALI
                     FFVVGVALIVLNHIFDFHKLLTRKKGTAEVVSNEPSESVEKNEE"
     gene            complement(4212..7052)
                     /gene="uvrA"
                     /locus_tag="PLU08_04275"
     CDS             complement(4212..7052)
                     /gene="uvrA"
                     /locus_tag="PLU08_04275"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOQ70938.1"
                     /translation="MDKKDYIIIKGARDNNLKNISVNIPKNKLVVFTGLSGSGKSTLA
                     LNTIYNEGQRRYVESLSAYARQFLGNLAKPDVDSIEGLSPAISIEQKSTSNNPRSTVG
                     TITEIYDYLRLLYARIGMPYCPQHDIPISSSSPEQILNKIFEYPEKTRIQILAPLVKA
                     EKGTHKDTLAKIAKEGFVRIRINGEIKALEEVGNLDKNKRHNIDLVINRVVLKAENKS
                     RIFEGINTALDYGHGYVIILFNNEETLFSTHNACHICGFSVPRLEPRLFSFNAPMGFC
                     PECRGLGLKREVDINILVPDKIVSLAKGAIEYYKNIVGTKNIEWQEFEKLCELYNIPM
                     HVPFKELSDKHKEIALFGSPNEHNFKIITGSGNTMYRRGKIEGVKNKIERLYEETTSE
                     FMRQYYEKFMRDVTCSTCNGKRLNEHALAVYINGKNIHDLTMLQIGELKQFLNETRPK
                     LSVTDRTISQLVLDEIDHRLTFLVKVGLDYLTLARSAATLSGGEAQRIRLATQIGSKL
                     TGVLYVLDEPSIGLHQRDNNKLIQALRDMVDLGNTLIVVEHDEETIRSADHIIDIGPG
                     AGIHGGEVVVTGTVEDVINCKKSITGDYLSGRKVIPLPPRRMPGNGNYLKIYGAAANN
                     LRHIDVSIPLNTLTLVTGVSGSGKSSLVEEVLFKAVDGHLRRVETYPGAYSKIEGLEH
                     IDKVINIDQDPIGRTPRSNPATYTKVFDDIRELFAQTVDARARGYTKSQFSFNTKGGR
                     CEHCEGAGVTRIAMNFLPDVYVKCEMCDGQRYHADTLSIHYRGKNIVDVLNMTVEEAL
                     EFFANRPQIVRRLQTLYDIGLGYIKLGQPAPELSGGEAQRLKLAFELQKRPTGKTLYL
                     LDEPSTGLHADDVGRLITSLQAIVANGDTVIVIEHNLDIIKVADYIIDLGPGGGNYGG
                     RVVASGTPEEIVKVKGSYTGEYLAPYLKKEKK"
     gene            complement(7165..>7539)
                     /locus_tag="PLU08_04280"
     CDS             complement(7165..>7539)
                     /locus_tag="PLU08_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70939.1"
                     /translation="EITIYRAPFEFSEKVQFTNVSATKDIKIDVYNLELMFKNVIARQ
                     KLTINSDYYAVIKLNNIDATKILIILNKGSVSGTLSSAKEFDLIGTFKNNIKCPAFLV
                     PNGTCTIKIKEEGKVNLKVLEH"
BASE COUNT         2306 a         1605 c         1171 g         2457 t
ORIGIN      
        1 ttagttatct aatgtactaa caaaaacata caaaaaaacg aatattttca cttattaggt
       61 aaaaatatcc cgaattccac tttttttaat caaaattagt tttattgaat gagttcaatc
      121 cctaaaagta aggatgataa agtaatacca aaggcttcac taatgcggta gagaagcgct
      181 aaagtggggt tacgtcttcc gttttctaaa tcacataaat aattgtgatt cacattcgcc
      241 cgtactgaaa gttctaaaag actccagccc tttgcttttc gtaattgccg taatctaatc
      301 ccaaaggctt catttatttc cacgacttaa ctccttaaag ggttctgcta gttcccgtaa
      361 cttaataaat aaatgattaa cattactttt agtaattctt ttttcacttc ctaattcttc
      421 tgttaacatt tccgcgagtt cattcattgt cgcgtcttca ttttctaaac gtaaacgcat
      481 cagtgctccg agtttttcat tattatgcgg agtatgtccg tacactcttt gtacaatctt
      541 aatgttttca atttgttcag tggcactttt aactgtttta ttgtaattag cttcgtcgca
      601 tataaacatc cggttcgtaa tattttgcat gtcgcggtcc attctgatcc cttcaaattc
      661 taaactagcg ttcgccgctc cgacaaagac aagaaacgac gaaatttggt cacttcgctt
      721 taagtacaca atatatttat cacgtcttgc agttacttta gcgttaaagt ttgtcttttc
      781 aaaacggtta agcattcgcg ctaagtgttc ggcgtatttc ttttggcgca caacaatttc
      841 taaatggtaa ttggaactta agggacttgt cattgaacca ctgcctaaaa aagcacccgt
      901 aaagtatcca cctaaagtat catcactata tacaatcttc cgactcacag cgctatcata
      961 aaaatcaacc gcaagtgttt caataatttc atcaaccttt tgttcaacaa tcacattaaa
     1021 tttcatcttt ttcccaaatt tacgttttgt aacatacgaa aatgttggtt caaaattaaa
     1081 gatatcttta aataattgat aaatatattt cgctacctta gcgttttccg tgctaagttt
     1141 taaaacttct ttcttctcac gaattattaa atgacctgca tgcttaatga gcgcggcaag
     1201 tgttgctttc cgtcttggta aggaatagga tattgtatta aaagtaatat cttcttttgc
     1261 tcttgtagta aatgacacat ttttcatact tatattataa aggaaaaggg ggaatttccc
     1321 ccaaatttca cttatttact aattagttat tttcgtttca caaaacgatt cgccgcgaga
     1381 gcagcaatcg caccgtcgtt tgtagcggtt acaatttgcc gtaatacttt ttgattcacg
     1441 tcaccaatac taaagagccc agggacactc gtcatcattt cttcattcac taacacaaac
     1501 cctttatcgt cacggacatc aaattcactt aagaaatccg cggatgcatc acttcccaca
     1561 aacgggaaca cggctttgac ttcaataact ttgtcggtgc catcaattac attcttaatt
     1621 gcaacactag tgacaccttt agtgtcccct aagatttcaa ctggaatata gtttaagtgg
     1681 aaagtaacgt ttggcattgc tttaagttca ttaacaaggg attcatctgc tctaaagcgt
     1741 tcattacgat gaacaatatg cacatgttta acgattttac ttacaaagag tgcttcttca
     1801 attgcactat taccagcgcc aataatcata atcgcatcat ttttatataa gttcccgtca
     1861 caaatagcgc agtaacttac acctttaccg gtgtaacgcg cttcacctgg tatttttagt
     1921 tttcgctctt gggtacccgt tgcgacaatg acagcttttg cgtaaagcgt ctcttcatca
     1981 ctatgaatta caaaataatc gtcttctttg gtaattttat ttacatcagc gtaacccact
     2041 tcaatcccta agctgcgtag ttgttcaaac attttaaagg caaggtcagg tccgctgatg
     2101 gtttcaatac ctggataatt ttcgacgtcc tgcgtaatat tcattttgcc gcctggcgca
     2161 tatttatcaa caatcttaaa tgtcgcattt gcgcgcgaaa gataaattgc cgccgtcatc
     2221 cccgccggac ccgcaccaat aattaaaatc ggaataattt ctttattcat cactttgcac
     2281 tactcctaag acatcactaa tgtcatctaa tacatatgtt gcacttacat caaggcgatg
     2341 cggacggcga tgttgatgga taaacgcaac atcgactcct gcgtcttgac cggctaagat
     2401 atcacgccaa ttatcaccaa caaaaagtgt tttatcaggt gcacttttta gcgttttcat
     2461 aatgtgcact aagccttcac cacttggttt ctttttaaaa ccgtcatcaa cacatacgag
     2521 cgttttaaag taggaatgaa caccgtgtct ttttaaaatt tgttcagtgc ttgctctagc
     2581 tctattagtg agtaaagcta actcaatatt attatttttt aaagttttaa ttaattcttt
     2641 cgctccttta aaaagcgtaa tatctttaac gctatctggt aagtattcat tatatttagc
     2701 tattaactct tctaaggggt aacctgggaa taagtacggc attgtttcgt caagcgttgg
     2761 cccgtttaaa aagagatagt catccatatt aatgtctttc cgcggtaaat aagtttcact
     2821 cattttaatc ccgattttaa cgagtcctaa atcactatcg gcaatcgtgc cgtcaagatc
     2881 aagcaccact aaatcgtatt tactcttcat ttttttctac actttctgaa ggttcattac
     2941 ttactacttc agctgttcct ttctttcttg ttaaaagttt gtggaaatca aagatatgat
     3001 ttaaaacaat gagggcaaca cccacgacaa agaagataag ggcattatac tctgaccact
     3061 taccacctgc tcccatgcgg aacattgggt tacgtaacgg ttccataata aaccgcacta
     3121 aaccatacca cacaatatac ataaatgcca tatcaaatgg tttacgatat ttcttaagtc
     3181 cttcaccaac accgtaacga atcacaaagt aacccgttaa attaagcgct gattcaataa
     3241 ggaagaaggg ttgacggtat ttccctaaaa taaacatttg gcgtttaata aattcaggga
     3301 gaaaccacca ttttgaaata tcagtaatat ccccaccata gacttcttga ttaaagaaat
     3361 taccccagcg gccaattgct tgagcaacta agatcgtcgg aataatgatg tctgcggcaa
     3421 aaccaatatc ataagcttta cgtcttttca caaagaacca caccccagca ataatcccaa
     3481 aaagcgctcc gcccataatc gctagaccac catctcgaat cgcaaaaatc gttaacggat
     3541 tttccgcaaa acctgattca ggattattcc attcaccaat cacataccat atgcgtgcgc
     3601 cgataattcc cgctggaaaa gcaacataga aaacattttc taaaataccg tgtctaccgt
     3661 attttttata aaattcgtgg tcagtaatcg ctaatactaa aagcgcacca gttagaatga
     3721 atattgcgta aaaagcgata acgggactat caccaaaagg gataccgcgt ggtaatggga
     3781 actttataat ttccgcaagt gttaagcctt caagcaatac aaaaaggaaa acaatactta
     3841 aaacaccgct tataatcgtt actattttac tttcttttat ccactctctt tgtaacacat
     3901 taaatttata atagtgtaaa acaaaagcag caataaaagt gagtaacatt aaagcaaaga
     3961 gaaaactacc taaaccaatt aacaaagaat gtaacacact atttgatagc accatcagtg
     4021 gtaaaaacag cattaacact gacactgcac cgcttaagac actaatggcg attaaacggt
     4081 gtttaccttt gaacaaggta ttaatatcct cttctttttt tacaaattca ttaagactta
     4141 aaccaaggac cgtcactgct ccaataaaga tcaagccaaa tattacaact accacgagtg
     4201 ttaaatccat attacttttt ctcctttttt aagtagggcg ctaaatattc tccagtatac
     4261 gaccctttaa ctttaactat ttcttctggg gtcccactag cgacaactct tccaccataa
     4321 ttcccaccgc ctggacctaa atcaattata taatcagcaa ccttaataat atcaagatta
     4381 tgctcaataa caataacagt atccccatta gcgacaatag cttggagtga agtaataagc
     4441 cgcccaacat catctgcatg taagccggtt gacggttcat caagtaaata tagtgtttta
     4501 ccagtcggac gcttttgtaa ttcaaaggca agttttaagc gttgtgcttc cccgccacta
     4561 agttctggtg caggttgtcc aagcttaata taacctaaac caatatcgta taaagtttgt
     4621 aaacggcgta caatctgcgg cctatttgca aagaattcaa gcgcttcttc gactgtcata
     4681 ttaagtacat caacaatgtt tttgccgcga taatgaatcg aaagcgtgtc agcgtgatat
     4741 ctttgtccgt cacacatttc acattttaca tagacatcag gtaaaaagtt catagcaatg
     4801 cgtgtaacac ccgccccttc acagtgttca catctaccac ctttagtgtt aaacgaaaat
     4861 tgactcttcg tgtaacctcg cgctctagcg tccacagttt gcgcaaaaag ttcacgaatg
     4921 tcatcaaaca ctttcgtata agtcgcaggg ttactgcgcg gtgttctacc aattgggtct
     4981 tgatcaatat taattacttt atcaatatgt tctaagcctt caattttaga gtaagcacct
     5041 ggataagttt caactctacg taaatgaccg tccactgcct taaaaagtac ttcttccact
     5101 aaacttgatt tgcctgaacc gctcacaccc gtgacaagtg ttaaggtatt aagcgggata
     5161 cttacatcaa tatgccttaa gttattcgcc gctgcgccat atatttttaa gtaattaccg
     5221 ttcccaggca tccgtctagg cggtagcgga attactttac ggcccgagag ataatcacca
     5281 gtaattgatt ttttacaatt aatcacatct tccacagtgc ccgttaccac tacttcacca
     5341 ccatgaatcc ccgctcctgg accaatatca ataatatgat cagcgcttct aattgtttct
     5401 tcatcgtgct cgacaacaat tagtgtattc cctaaatcaa ccatgtcgcg taatgcttgg
     5461 attaatttat tattatcacg ctgatgaaga ccgatacttg gttcatcaag cacatataaa
     5521 acaccggtta atttactacc aatctgcgtc gcaagtctta tgcgctgtgc ttcgccacct
     5581 gaaagcgtcg cagcgctcct cgcaagtgtt aagtaatcta agccgacctt tactaagaaa
     5641 gttaaacggt gatcaatttc atcaagtaca agttgactaa ttgtcctatc tgtcacactt
     5701 agttttggtc gcgtttcatt taagaattgc tttaattcac caatttgtaa cattgttaag
     5761 tcgtgaatgt tcttaccgtt aatatacacc gctagagcgt gttcgttaag acgcttcccg
     5821 ttacatgtcg aacaagtaac atcacgcata aacttctcat aatattgacg catgaattca
     5881 cttgttgttt cttcataaag ccgttcaatc ttatttttaa cgccttcaat ctttccgcgt
     5941 cgatacattg tattgccgct accagtaatg atcttaaagt tatgttcatt tggactacca
     6001 aaaagcgcta tttctttatg tttatcactt aattctttaa atggcacatg cattgggatg
     6061 ttatagagct cacataactt ttcaaattct tgccattcaa tatttttagt accgacaata
     6121 tttttataat attcaatcgc ccctttagca agacttacaa ttttatccgg cactaaaatg
     6181 ttgatgtcaa cttcacgctt aagtcctaac ccgcgacatt ctggacaaaa acccattggg
     6241 gcattaaagc tgaatagtcg cggttctaaa cgcggaacac taaaaccaca gatgtgacac
     6301 gcgttatgcg tagaaaaaag tgtttcttca ttattaaaaa gaataataac gtacccatgt
     6361 ccataatcta gcgcggtatt aattccttca aagatgcgtg atttattttc ggctttgagc
     6421 acaacacgat taatgactaa atcaatgtta tgacgtttat ttttatcaag gtttcctact
     6481 tcttcaagtg cttttatttc accattaatt cgaatccgca caaacccttc ttttgcaatc
     6541 tttgctagtg tatctttatg tgtacctttt tcagctttga caagtggcgc taaaatttgg
     6601 attcttgttt tctctggata ttcaaagatt ttatttaaga tttgttcggg tgaggaacta
     6661 ctaattggaa tatcgtgctg cggacagtaa ggcattccaa tccgtgcata taaaagccgt
     6721 aagtagtcat aaatctccgt aattgttcca actgttgaac gcgggttatt actcgttgac
     6781 ttttgttcaa tgctaattgc gggactaagc ccttcaattg aatcaacgtc aggcttagcg
     6841 agattcccta aaaattgccg agcgtaagca cttaagcttt caacgtaacg cctttgccct
     6901 tcgttatata ttgtatttaa cgctagggtt gattttccgc taccggaaag accagtgaac
     6961 accactagtt tatttttggg gatattcaca ctaatgtttt ttaagttgtt atccctagcg
     7021 cctttaataa taatgtagtc ttttttatcc atagctttca ttttacctta ttcataaggt
     7081 atttagacaa attatgcacg gtaagtaaaa aaggttaaga cacaatgccg ttaaccactt
     7141 taaaataaaa ggcgaaaatt tatattaatg ttcaagaact tttaagttta ctttgccttc
     7201 ttctttaatt ttaatcgtac atgttccatt tggtactaaa aatgcggggc atttaatatt
     7261 atttttgaaa gtacctatta aatcaaattc tttagcactt gataaggttc cactaacact
     7321 acctttattt agtataatta gaatctttgt agcatcaata ttatttaatt taataaccgc
     7381 ataataatca ctattaattg taagcttttg tctagcaata acatttttaa acattagttc
     7441 aagattataa acatctattt ttatatcttt tgtggcgcta acattagtaa actgaacctt
     7501 ttcactaaat tcaaatggtg ctctataaat tgtaatttc
//
LOCUS       DAOSTA010000043         7415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_9325_length_7415_cov_8.462364, whole genome shotgun sequence.
ACCESSION   DAOSTA010000043 DAOSTA010000000
VERSION     DAOSTA010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7415
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9325_length_7415_cov_8.462364"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..928)
                     /locus_tag="PLU08_04285"
     CDS             complement(197..928)
                     /locus_tag="PLU08_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70940.1"
                     /translation="MQNENKTQKFQINKTFIIKLIVYLLGIILVGFGVQIIIYTNVGT
                     APIDAMNYYLARIYLQLFNNSDFSKLKTTIGVASLAFGTLTVLVLFIITKNKALIFTW
                     LNIFIVSLVISIWGLLFDTFNPLNTTFFIRALVALLGILIISFGVFLVIVTEFPAGPP
                     EEIMRLINKKTNNLFVAKLLAETFYLVLACISMIISVTIIDGNGVEFTQVGIFTIVTL
                     ILTSVFVSLFDLAYRKLVVKKVESA"
     gene            complement(1021..3207)
                     /locus_tag="PLU08_04290"
     CDS             complement(1021..3207)
                     /locus_tag="PLU08_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ70941.1"
                     /translation="MRILELNIDSFGKLENYNLKFDQKLTIINEDNSFGKTTIAQFIK
                     AMFFGMKTAKGKGKRLSLPRFKYLPWGKTTYGGNLTFVYNGRTYTVTRTFGESLSNDT
                     IAVKDLARNVNIDELNGIKITRDNLGDIIFGVNSESFEKLNFIQQLEVLYSSDEKIHD
                     DINVKLRGLFGHTTEDSDYTSAFNILDDAIKELSPGNQRRNSLYNKTDRELNATNNLV
                     FEAENAVNLISENSDELVALKRQKAENDKLKTKVENDLKLVANKKILEKDLKDYNALN
                     DEINNLNLQIEENKKLFAGNKVEDTNLVYLEELSTDLSAKESDFNKFKDEAQTEINSI
                     NAALTTLESTNVEVLFIKRAHKETSEEITKLENEVTLLNANIKRFRFLDIFLSIITLS
                     IYFFVLKSKNNKLRKEIALLNKTIDELNVKLENYAAELATMTSTESDIKLKQKLDEWT
                     QKVNQKQKELEAFKEHVFDVFRKYSVKVNSVSDVQVAYFLINSHYEKHLALNKKLNDV
                     NFKLKNYDIKKINSDLNSFALLDEEELNRNKSNVDIKNDELIKKITALEKQIEANEDL
                     ASNLENYKFERDELRTTMQNYEQKKELLTIARTLLEHANQQLAARYLTPLQNNVNDLL
                     TKIKLEGYSVMFDGNSEMKLKDLDTNEYYDFAYYSAGSQDLISVLVRVALIDLVYQDL
                     KPFIILDDTFVNFDDKRIKLVRPLLEKLSQDNQIIYFTCSSSRDLR"
     gene            complement(3204..4265)
                     /locus_tag="PLU08_04295"
     CDS             complement(3204..4265)
                     /locus_tag="PLU08_04295"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HOQ70942.1"
                     /translation="MKIIHTADIHIDSKMNRYGGDKTERRIREIKNAFFRMIDYGVKH
                     NVKVILIAGDLFDNKEVKLATLREVLTKITSAPTIDFIYLAGNHDDPAVFNHEKLAEL
                     PSNLKIVTKENNTFLYDDVCIKAYDNYADEDYTKTLIFDPNKYNILMLHGTEAEIPLN
                     NLKGKNIDYLALGDIHIPDIAAKRLDARGIYAYSGVLEPRGFDEVGERGFFVLEIQKN
                     KLIRREFIKNNSRTYHIIDVDISALNNTLEIELAIKKATNKIPSDDIVRLKLTGKYDY
                     ELIKDINALREYLESKFYYAELIDDSKVNLNTIDFANEVSLRGEFFRLIEASNYSPEV
                     KEELLKLGLNALKGRKIDL"
     gene            complement(4427..5761)
                     /gene="mgtE"
                     /locus_tag="PLU08_04300"
     CDS             complement(4427..5761)
                     /gene="mgtE"
                     /locus_tag="PLU08_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOQ70943.1"
                     /translation="MKNLMILNDAALKKTLAKMHPYDIATKMKDASGDTQMRLIRLMA
                     LNKTVEVFLELPSEVSRHYFSLLTETQKKQFLEAFEMDDLKTFITSFEDDYQMYLLSL
                     LGTHKAIALKVLMSYDDEKIATIMSTNFVTITQNSTIKDTTAHIINSSKYNDFVDDIF
                     VINENNALIGSITLKDLISARATDTLDSITYSINNYLTLDDSLNDGYKKFENYAKSVL
                     PVLDDDMKIVGIVTADDVLNIMVAEHEEDIAKMTGVGDYDESSNAFRRSIQRMPWLVI
                     SVLLNLVIAALLSVFEKTLVEVIALIMFQPMILGMAGNIGTQSIAVTILKLNQDELAG
                     IHNEKKHIGKELAIGVLNSITIGLGGFLLSFLILSFINMGTHSPLLLAITIGTSLMGG
                     MFISAAAGVFIPIILEKMKVDPAIASGPIISTLNDLFALLIYFGIATLIFLL"
     gene            complement(5762..7066)
                     /locus_tag="PLU08_04305"
     CDS             complement(5762..7066)
                     /locus_tag="PLU08_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOQ70944.1"
                     /translation="MRTIIKRKDKKALLKYVKKMHPYDIIEALNTLTLDEQELFYSLL
                     SEEEIGRIVAFMEPEKAAEVIDDFDLTKQKNIIDTLELDDAADIIVYLDNETKLIEGL
                     EKELHITQVLKYDEEKVGAHMSDAYISLSLGTDIKAATKNVIQKAGEIDVINHIFIVD
                     SGNKYKGTVELRDLVRTKAPALIDDITEFTPFVFASDDIEESVHKMKNYGLHEMAVVS
                     EELELIGIITIDDAIEIYHEESVDDFEKLSALPDTKTKGVIKSAAKRLPWLFLLLVLS
                     MPIAIATSRFEEVITSVAVLALFQPLILDAGGDVASQTLAVTLRLLSKNPKKALRNGG
                     TEILTGIINGLGLGIVAGIVSFIISKVIGITEPFNVSLVVFISLAVTVATGPIFGLFI
                     PLILDKMKIDPAVASSPLITTLIDITSVVIYFGVATLLLGVI"
     regulatory      complement(7141..7306)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
BASE COUNT         2363 a         1275 c         1114 g         2663 t
ORIGIN      
        1 aacattataa tgaagacaag aaggatgtaa atttaacctt ttcctttaac gattaagaat
       61 atccttacaa ttataattga ttcaccccaa acgactgttg ggtcccccac tcttcggatt
      121 cggagtcccc tatatcttat tagatatagt atggtaagtc aatttttttt ggtgcgtgac
      181 tataaaaaaa tccgcgttaa gcggattcta ctttctttac gactagtttt cgatacgcta
      241 aatcaaataa tgatacgaac acagaagtta aaatcagtgt cacaattgta aatataccaa
      301 cttgggtaaa ctccacacca ttaccatcaa taatcgttac tgagataatc atacttatac
      361 atgctagaac taaataaaaa gtttcggcta ataatttagc gacaaaaaga ttatttgttt
      421 ttttattaat tagacgcatg atttcttcgg gtggaccagc agggaattct gtgacaatca
      481 caaggaaaac accaaaagag ataattaaaa tcccaagtaa agcaactagt gcacggatga
      541 aaaatgtagt attaagcggg ttaaatgtat caaaaagtaa accccaaatg gaaattacga
      601 gtgaaacaat aaatatattt agccatgtaa aaattaaggc tttattttta gtaataataa
      661 ataagacaag cactgttagt gtcccaaacg ctaatgaagc aacaccgatt gttgttttaa
      721 gtttactaaa atcactatta ttaaaaagtt gtaagtaaat tcttgctaag taatagttca
      781 tcgcatcgat tggtgctgtt ccaacatttg tataaataat tatttggaca ccaaaaccaa
      841 ctaagataat gcctaaaaga tatacaatta gcttaataat gaatgttttg ttaatttgaa
      901 acttttgggt tttattttcg ttttgcatac ttcgtaatta taacaatttt atagtgctaa
      961 tacttaaaat ataaatgtaa aaatacatga gttagttgta tttattcatt gtaaatttta
     1021 ttagcgtaag tccctactgc tagaacaagt aaaataaata atttgattgt cttgtgataa
     1081 tttttcaagt aggggtctta cgagtttaat tcttttatca tcgaagttaa cgaatgtatc
     1141 atcaagaatg ataaatggtt ttaagtcttg atacactaaa tcaattaagg caacgcgcac
     1201 taagaccgaa ataagatctt gactaccagc actgtaataa gcaaagtcgt aatattcatt
     1261 cgtgtctaaa tcttttaatt tcatttcaga gtttccgtca aacattacag agtagccctc
     1321 taatttaatt ttcgtaagta agtcgttaac attattttga agcggggtta aataccttgc
     1381 tgctagttgc tgattagcgt gttctaataa tgttctagcg atagttaata attctttttt
     1441 ctgctcatag ttttgcattg tagtgcgtaa ttcgtcgcgt tcaaacttgt aattttcaag
     1501 gttactcgct aagtcttcat ttgcttcaat ttgtttttct aaagctgtta tttttttgat
     1561 taattcatcg tttttgatgt cgacattact cttattcctg tttaattctt cttcatctag
     1621 tagggcgaaa gaatttaaat cactatttat ttttttaata tcataattct taagtttaaa
     1681 atttacatca ttaagctttt tattaagcgc taaatgtttt tcatagtgac tattaataag
     1741 gaaataagca acttgaacat cactaacact attaacttta acggaatact tacgaaatac
     1801 atcaaaaaca tgttctttaa aagcttctaa ttctttttgt ttctggttaa ctttttgtgt
     1861 ccactcatca agtttttgtt taagtttaat gtcactttct gtgctagtca ttgtcgctag
     1921 ttcagcggca taattttcaa gttttacatt aagttcatca atcgttttat ttaaaagcgc
     1981 tatttctttt cttaacttat tattcttact tttaaggaca aagaaataaa ttgatagcgt
     2041 gataatgctt aggaaaatat caaggaaacg aaatcttttt atatttgcat taagtaaagt
     2101 tacttcattt tctaatttag taatttcttc actagtttct ttatgcgctc ttttaataaa
     2161 taggacttca acatttgtac tttcaagagt agttagagca gcgtttattg aattaatttc
     2221 tgtttgtgct tcatctttaa atttattaaa gtcactctct tttgccgaca aatccgtgct
     2281 taattcttct aaatacacta aatttgtgtc ttcaacttta ttacccgcaa ataatttttt
     2341 attttcttca atttgtaaat taaggttatt tatttcatca tttagtgcat tataatcttt
     2401 aaggtctttt tcaagtatct ttttattcgc aacgagtttt aaatcatttt caactttagt
     2461 ttttagctta tcattttctg ctttttgtct ttttagcgca actaattcat cagagttttc
     2521 actaattaaa ttaacagcgt tttctgcttc aaacacaaga ttatttgtgg cattgagttc
     2581 acggtcagtt ttgttatata gtgaatttct tcgctggtta ccaggtgaaa gttcttttat
     2641 agcatcatca agaatattaa atgcggaagt gtaatcacta tcttctgttg tatgcccaaa
     2701 cagaccgcgt aattttacat taatatcatc atgaattttt tcatcactac tatataaaac
     2761 ttctaattgt tgaataaagt ttaatttctc aaaactttca ctatttacac caaagatgat
     2821 atcaccgagg ttatcacgcg ttattttaat accatttaat tcatcaatat ttacattgcg
     2881 cgctaagtct ttaactgcaa tcgtatcatt agagagcgat tcaccaaagg tgcgggtaac
     2941 agtataagtt cgcccgttgt agacaaaagt taagttacca ccataggtgg ttttacccca
     3001 cggtaaatat ttaaagcgtg gtagtgatag tctttttcct tttcctttag cggttttcat
     3061 cccaaagaac atcgctttaa tgaattgcgc aatcgttgtt ttaccaaaac tattatcttc
     3121 attaatgatt gtaagctttt gatcaaattt aagattgtaa ttttcaagct taccaaaact
     3181 atcaatatta agttctaata ttctcatagg tcaattttcc ttccctttag tgcatttaga
     3241 cccaatttta agagttcttc cttaacttca ggggagtaat ttgaagcttc gattaggcga
     3301 aagaattcac cgcgtaatga tacttcgttt gcaaaatcaa tagtgttaag gttaacctta
     3361 gaatcatcga ttaactctgc ataataaaat ttactttcta aatattcgcg cagtgcattt
     3421 atatctttga ttaattcata atcatattta ccagttaatt ttagacgtac gatatcatca
     3481 ctaggaatct tattagtggc cttcttaatt gctaactcaa tttcaagagt gttgtttaat
     3541 gcactgatat cgacatcaat aatatgataa gtacgactat tatttttaat aaattcgcgt
     3601 ctaatcagct tatttttttg tatttctaaa acaaaaaagc cgcgttcacc cacttcatca
     3661 aacccgcgcg gttcaagcac acctgaataa gcatatatgc cgcgcgcatc taatcttttc
     3721 gcggcaatat cggggatatg gatgtcgcca agcgctaagt agtcaatatt tttgcctttt
     3781 aaattattta aagggatttc agcttcagtg ccatgtagca tcaagatgtt atatttattt
     3841 ggatcaaaga ttaaggtttt agtgtaatct tcatctgcat agttatcata agctttaata
     3901 catacatcat cgtaaaggaa tgtattattt tcttttgtca caattttaag atttgaaggt
     3961 aattccgcta atttttcatg attaaataca gctgggtcat catgattacc cgctaaatat
     4021 ataaaatcaa ttgttggtgc acttgtaatt tttgttaata cttcacgtaa ggtagcaagt
     4081 tttacttctt tattgtcaaa taagtctcct gcgattaaga taactttaac attgtgctta
     4141 actccgtaat cgatcatgcg aaaaaaagca tttttaattt ctctaatgcg tctttctgtt
     4201 ttatcgccac cataccgatt catctttgaa tcaatatgaa tatccgctgt atggattatt
     4261 ttcatactta cccccgttaa cttacattta gttaacttat tttatcataa tacctagcca
     4321 ttaaagtgaa tatagcatta ataaatgcta ttagccttca ttaataaaaa aagacatcat
     4381 gtggcgatgt cttttatatt gtttgtaagc atctcttctc ttgctcttat agtaagaaga
     4441 ttaatgttgc aattccaaaa taaattagta atgcgaataa gtcatttagc gtcgagataa
     4501 ttggaccact agcaatcgca ggatcaacct tcatcttctc taaaataatt gggataaaaa
     4561 caccagcggc agcagagata aacatcccgc ccataagcga agtcccaatc gtgattgcta
     4621 gaagtaacgg gctatgcgtt cccatattaa tgaaactaag aataagaaat gagagtaaaa
     4681 aaccaccaag accaatagtg atgctattta agactccgat tgctagttct ttgccaatgt
     4741 gttttttctc attatggata cctgcaagtt catcttgatt taattttaaa atcgtcacgg
     4801 caattgattg agtgccaatg tttcctgcca tccctaaaat cattggttga aacataatta
     4861 aggcgatgac ttcaacgagt gtcttttcaa atactgataa tagggcagca atcactaagt
     4921 ttaataatac tgaaataact aaccacggca ttctttgtat agaacgtcta aaggcattgg
     4981 atgattcatc ataatcacca acccctgtca tcttagcgat gtcttcttca tgttcagcga
     5041 ccataatatt taatacatca tcagcagtta caatcccgac aattttcata tcgtcatcta
     5101 gtactggtaa gacacttttt gcatagtttt caaacttttt ataaccatca ttaagtgagt
     5161 catcaagtgt taaataattg ttaatgctat aagttatgct atcaagtgta tcagtggcgc
     5221 gtgcactaat taaatctttt aaagttatac ttccaattaa agcattattt tcattaatta
     5281 caaagatatc atcgacaaaa tcattgtatt ttgagctatt aatgatatgc gcagtagtat
     5341 ctttgatcgt tgaattttgt gtaatcgtta caaaattagt gctcattatt gttgcaattt
     5401 tttcgtcgtc ataactcatt aaaactttaa gtgctatagc tttatgtgtc cctagtaaac
     5461 ttaatagata catttgatag tcgtcttcaa aactagtaat gaatgttttt aaatcatcca
     5521 tttcaaaagc ttctaagaat tgcttttttt gtgtttccgt taataaacta aagtagtgac
     5581 gcgaaacttc acttggtaat tcaaggaaca cttcaactgt tttatttagt gccatcaaac
     5641 gaattaatcg catttgcgta tcgccggaag catccttcat ttttgtcgcg atgtcataag
     5701 gatgcatttt cgctaaagtt ttctttaagg cggcatcatt taaaatcatt aagtttttca
     5761 tttaaatcac ccccagtaat aatgttgcta ccccgaaata aataacaaca ctagtaatat
     5821 caataagtgt agtaataagt gggctagaag cgactgctgg atcaattttc atcttgtcta
     5881 aaataagcgg gatgaataat ccaaatattg gtccagttgc gaccgtcacc gctagtgaaa
     5941 taaagacgac taatgataca ttaaagggtt cagtaatccc aattactttg gaaataataa
     6001 aagagactat acccgcgact ataccaagcc caagcccgtt aataattcct gttagtattt
     6061 cggtgccacc atttctaagc gctttttttg gatttttact aagtaatctt aaagttaccg
     6121 caagggtttg actcgcaaca tcacccccag cgtcaagaat taatggttgg aaaagggcga
     6181 gtaccgctac tgaagtaatt acttcttcaa agcgacttgt agcaattgca attggcattg
     6241 atagcactag tagtaagaag agccacggta atcttttagc agcggactta ataactcctt
     6301 ttgtttttgt atctggtagt gccgacagct tttcaaaatc atcaacggat tcttcatgat
     6361 atatttcgat cgcatcatca atcgtaataa taccgataag ctctaattct tcgctgacaa
     6421 ctgccatttc atggagacca taattcttca ttttatggac tgattcttca atgtcatcac
     6481 tggcgaaaac aaacggagta aattcagtga tatcatcaat aagtgctggt gctttagtgc
     6541 ggactaaatc tcttaattca accgtccctt tatatttatt gccactatca acaataaaga
     6601 tgtgattaat aacatcaatt tccccagctt tttgaataac atttttagtt gccgctttaa
     6661 tatctgttcc taaagaaagg ctaatatatg catcagacat atgggcgcca actttttctt
     6721 cgtcatactt taatacttgc gtaatatgaa gttctttttc taaaccttca attaacttag
     6781 tttcattatc gagatagaca ataatgtcag cagcgtcatc aagttcaagc gtatcaataa
     6841 tatttttttg ttttgttaaa tcaaaatcat caataacttc tgcagctttt tctggttcca
     6901 taaaagcaac aattctgcca atttcttctt cgcttagcag tgagtaaaat agctcttgtt
     6961 catcaagagt aagtgtattt agtgcttcaa taatgtcata aggatgcatt tttttgacat
     7021 atttaagcaa tgcttttttg tcttttcgtt taataattgt gcgcatgtta acctccctcc
     7081 tttgcttgga attcaaataa aaagctccca cattaagtga gagctaacta aacagtacga
     7141 gtatcagcgt attcgtagag ttttagcact atgcaacgta aacatgaagt tagctacttt
     7201 gtggaaagcc ttaagtcgac aatccatgtt ctacccattg gtgtctctgg acatttttgg
     7261 gcagcagcct atatttgcat aggagcctca cctaacggat ttatttaatt ccaaagtcca
     7321 taatagcata tatattatat ttgtcaatct actattttaa aaataataga agaaaaggtt
     7381 aaattgacaa gataaaaaac tgaaagaatc atttt
//
LOCUS       DAOSTA010000044         7333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_9458_length_7333_cov_9.268343, whole genome shotgun sequence.
ACCESSION   DAOSTA010000044 DAOSTA010000000
VERSION     DAOSTA010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9458_length_7333_cov_9.268343"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLU08_04310"
     CDS             complement(<1..396)
                     /locus_tag="PLU08_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70945.1"
                     /translation="MKKRNIFTKFSLTAILLSALFAFSSVRASSNIVRTEAWSGTQTS
                     TEGTYYSTVGSETGTALLNKLNTITGNPTPQTSYDWYRYEAADEAENESNKVLLIYSR
                     QKVLKTAHVSGSTGWNREHTYPDSKISGAA"
     gene            complement(458..3145)
                     /locus_tag="PLU08_04315"
     CDS             complement(458..3145)
                     /locus_tag="PLU08_04315"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOQ70946.1"
                     /translation="MEQKSIKQTLEQLNSCRENGLTTEEALKRQEVHGKNELPKAKKK
                     TIVGVFFSQMNNAMIYILLVAAVISFVMALIEGDGSFFEPILILAIVLLNAVIGAIQE
                     LKADKALEALEKLSAPTTVVRRDGELKEIKASELVPGDIVIIEDGRTIPADLRLISSA
                     NLKVSEASLTGESVPVEKDANIVYDRPMPIGDRKNVVYMSTPVVYGRGEGVVIGTGLQ
                     TEIGQIAKMLQTTKQEETPLQKRLADLSKLLGIITFVLVALLFIIALIKDPTWDNIKN
                     MFLLSISLAVAAVPEGLPAVVTITLALGVQRMVKVHTITRRLPSVETLGAVSVICSDK
                     TGTLTQNVMTVVSAYTNEKQYEVEDFSADTVGELADGLSLCSDASVDHGVYGDPTEIA
                     LVNFANLIDHHKDDLEAATPRVEELPFDSVRKMMSTLHKTGKTYTQYTKGAMDNVLEL
                     TTHISVNGKVREIKKSDITAINDVASKMAKRALRVLALAKKEVPFAGISEENLVFVGL
                     VGMVDPPRPEAIPAVENLKGAGITTIMITGDHADTAYAIAHELGITDSPSNVLTGAEL
                     DALNEDALKDALLTKRVFARVSPENKVQIVNALQANGEIVAMTGDGVNDAPSLKTADI
                     GIAMGITGTDVAKGAADMVLTDDNFASIEKAVQEGRGIYANIRKTVWFLLSSNFGEVI
                     TMLVAVLVGYPFPLSALHILWVNLITDGVPALALGADSKDPDIMKEKPRDKKESLFAN
                     GGYFITIFFGSVIALITTLAFLFIPVVQHGMRSVQDINTFFAENPELKVRAMTYAFTA
                     LGVSQLFHMFGMSNLNKTFFRTFTKKKWFLWVAFFIGFLLQIAVTEIHALTVAFGTVE
                     LTFIEWVFLALLSTTPLVIHELRVFFRFIREKIKNRKSH"
     gene            complement(3289..4788)
                     /locus_tag="PLU08_04320"
     CDS             complement(3289..4788)
                     /locus_tag="PLU08_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70947.1"
                     /translation="MRTLFLANTWDFNPFWISIFQIGVVGALILIANTIRRKTPFLRN
                     YLVPTGLIAGFLGLGLKYLFQWLGVEIYGTPIVDESYMHFLTYHSLAIGFIALGLVTA
                     EKKVEKEGRALKSGALIVGGYLIQGIVGLILSLGVSLIFVNYAIGKAPYAGVLLPLGF
                     GEGPGQAGNMGDVYEKLTDYNALAGGRDFGLSIAAMGFVVAAVVGTVILNVVARKGLV
                     TRRKAEVTETFGGLADSQYAVVDHPEEIPVVESVDKFTIQVAFVIAIYLIAFGIMSLV
                     SFLVVDLAKFASIRGIIWGFNFLFAIITTMIVKAVLNLLRKKKIMKRKYINNFMQNRI
                     AGLAFDVMIAAGIMSINFGKLNDPSFWVLLALMSVFGTIVSYYYINFVAKRYFPKTRW
                     YTFFGFFGMLTGTMSEGVALLREIDPYFETGVAEDLVNGSGTAALFGVPMLLITTVVY
                     RGWVGFWVSFALLIVLFAAMILVLHFVTKSRNKKLGAETNGEKEVLETE"
     gene            complement(4822..6090)
                     /locus_tag="PLU08_04325"
     CDS             complement(4822..6090)
                     /locus_tag="PLU08_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOQ70948.1"
                     /translation="MRNRKPKKQRIVKKRHQRYVNLANFLLGRLIRRSYGVKQAKIDI
                     KQYEPCLIVSNHILTFDPILMTSIFGHQIYYVASELIFSLGWKSRLLTHCFAPIPKTK
                     SMADVTTVKNIMQIVKEGGSVGVYVEGNATMTGGLSSMPAAIGKLVLRLKIPLVIFNF
                     EGGYLSLPRWATYTKREKHVRGKIRKVLYYEDYKDMTADEINELIIENININAYEESP
                     QYTYRGKRNAEGLHRLLFTCPICHGVNTAKTAYNNYSCAKCGEILTYDEHGYLHSTKW
                     DKPKNLIELDKANNLAYQEHIINNPNFSVSVPGNFTEVFRKRRKHFGPATLTLSREGL
                     TIKFKRKMKRHVTQHFTIDELDTMAMQQKEVLVIYPRHEQTKMLKIPKTEIVSSYQFV
                     VTLQILKNILQYPDAKPDEVPRLSAQDLGL"
     gene            complement(6080..7219)
                     /locus_tag="PLU08_04330"
     CDS             complement(6080..7219)
                     /locus_tag="PLU08_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70949.1"
                     /translation="MIKIVPVYGGRKLRQFINFPVKLYKNSKQFTPYIFEDEISNLTP
                     SKNPASAWCDFKLFLAYRDRKIVGRICGIISHYANKKYDQKRVRFNRIDMIDDIEVTK
                     ALIKAVEEWGYSRGMNEIVGPLGYSDQDKEGLLVEGFEYSNMFATFYHFPYYMKHLEE
                     LGFVSDAVWVEHRIIIPKEIDERFERVSEMVKRKYNVKVRRLKSKRQIQPYVRKALAL
                     TNRAYDHLYGYVPMSEKLMDMLARQYVPLINLKYLILVVDEKDELLAFGLSIPSPVFA
                     LKKIKGKLFPFGFIRFLRALKKSKQLDLLLVAVEPELQNSGLANVIMYESMKSAIADG
                     IEYAETGPQLEYNKPVQRLWKHLNYFNHKRRICYVKPITEVEGEE"
BASE COUNT         2333 a         1531 c         1162 g         2307 t
ORIGIN      
        1 ggcagcaccc gatattttgg agtcaggata agtatgttct ctattccaac ctgtacttcc
       61 tgaaacatgg gccgttttaa gtactttttg tcgcgaataa attaaaagca ctttattgct
      121 ctcattttcc gcctcatccg ctgcttcgta gcgataccaa tcataactag tttgcggagt
      181 cggattaccc gtaattgtat ttaatttatt aagtaaagct gtgcccgttt cactaccaac
      241 ggtggagtaa tatgtacctt cagtactagt ttgtgtcccg ctccacgctt ctgttctaac
      301 tatattacta ctagcgcgta cactactaaa agcaaagagc gcagatagta aaattgcagt
      361 taaagaaaat ttagtaaaaa tatttcgttt tttcatatac ttatcctcac aaggaaattt
      421 attataactt tactaatgtc ataaagcaat agtgttatta atgacttttt cgatttttga
      481 ttttctcacg gataaagcgg aagaaaactc ttaattcatg aattacaagt ggtgttgtac
      541 taagtaatgc taaaaatacc cactcaataa atgttaattc aactgtacca aacgccacag
      601 ttaaagcatg aatttcagtt accgcaattt gtaaaaggaa gccaataaag aaagcaaccc
      661 ataagaacca ctttttcttc gtaaatgttc taaagaatgt tttatttaaa tttgacatgc
      721 caaacatatg gaagagttgg ctaacaccta atgcagtaaa agcataagtc attgctctaa
      781 ctttaagttc aggattttcc gcaaagaaag tattaatatc ttgaacacta cgcatcccgt
      841 gttgtactac cgggataaat aagaacgcca aagtagtaat taaagcaata acacttccga
      901 agaatatcgt aataaagtaa ccaccattag cgaataatga ttctttctta tcacgcggtt
      961 tttctttcat aatgtctggg tctttcgagt cagcgccaag tgcaagcgcc ggtacaccat
     1021 cagtaattaa attaacccat aaaatatgta atgccgaaag cgggaaagga taacctacta
     1081 agacggcaac aagcatcgta ataacttcac caaagttact tgataataaa aaccacactg
     1141 ttttacgaat attagcgtat ataccgcgtc cttcttgcac cgctttttca atactcgcaa
     1201 agttatcatc agttaatacc atatcagcgg cacctttagc cacatcagtt ccggtaatac
     1261 ccatcgcaat cccgatatca gcagttttta aactaggagc gtcattaaca ccgtcccccg
     1321 tcatcgctac aatttcacca ttagcctgta aggcattaac aatttgtact ttgttttcgg
     1381 gagatacacg ggcaaagaca cgcttagtaa gcaaagcatc ttttaaggca tcttcattta
     1441 aagcgtcaag ctccgcgcca gttaaaacat tacttgggct atcagtaata ccaagttcat
     1501 gggcaatcgc gtaagctgta tcagcgtgat cgccagtaat cataattgtc gtaatacctg
     1561 cgccttttaa attttccaca gcaggaattg cttctggacg cggtggatca accattccta
     1621 ccaagcctac aaaaacaagg ttttcttcac ttataccggc aaaaggcact tctttcttcg
     1681 ctaaagctaa cacacgaagt gcacgtttag ccattttact agcgacatca ttaattgccg
     1741 taatatcact ttttttaatt tcacgaactt taccattaac actaatatgg gtcgtaagtt
     1801 ctaaaacatt atccattgcc ccttttgtgt attgggtata tgttttacca gttttatgta
     1861 atgttgacat catctttctt acactatcaa atggtaattc ttcaacacgc ggtgttgccg
     1921 cttctaaatc atctttatga tggtcaatca gattagcaaa attgacaagc gcaatttccg
     1981 ttgggtcgcc ataaactccg tgatcaacac ttgcgtcact acaaagacta agaccatcgg
     2041 ctagttcacc gacagtgtca gcactaaagt cttccacttc atactgtttc tcattagtgt
     2101 atgcactaac aacagtcatc acattttgtg ttaaagttcc agttttatca ctacaaataa
     2161 cactgacggc accaagcgtt tcaacgctcg gaagtcttct ggtaattgta tgaactttaa
     2221 ccatccgttg cacacctaac gctagtgtaa ttgtcacaac cgctggtaag ccttctggga
     2281 ccgccgctac tgctaaagaa atagaaagca ggaacatatt tttaatattg tcccaagttg
     2341 gatctttaat aagggcaata ataaataaaa gcgcgactaa tacaaaagtt ataatcccta
     2401 ataattttga taagtcagca agcctttttt gtaatggtgt ttcttcttgc ttcgttgttt
     2461 ggagcatctt cgcaatttga ccgatttcag tttgtaaacc agtaccaatt acaacccctt
     2521 caccgcgacc atagacaaca ggagtcgaca tataaacaac atttttacga tcaccaattg
     2581 gcattggacg gtcataaaca atgtttgcgt ctttttccac aggaacactt tcacctgtaa
     2641 gactagcttc gctaacctta aggtttgcgc tactaattaa gcgtaagtcc gcgggtatcg
     2701 tacgaccatc ttcaataata acgatgtcac ctggaacaag ttcactagcc ttaatttctt
     2761 taagttcacc atcccgtcgt acaactgttg taggggcact aagtttttct agtgcttcaa
     2821 gtgctttatc cgcttttaac tcttgaatag ctccaattac tgcattaagt aatacaatcg
     2881 ctaaaattaa aataggttca aaaaaggaac catccccttc aattagcgcc atcacaaaag
     2941 aaataacagc agcgacaagt aatatataaa tcatcgcatt attcatctgc gagaaaaaga
     3001 caccaacaat tgttttcttt ttcgcttttg gtaattcgtt tttcccgtgc acttcttgac
     3061 gtttaagtgc ttcttccgtc gttaaaccat tttcgcgaca cgaatttagt tgttcgagtg
     3121 tttgtttaat tgatttttgt tccatatgat cctccaggac tagtgacaat tcatctaata
     3181 agtccaaaaa tagcgtacgt tgatatttta ctacaaatgt aatcatttga gtaaattagt
     3241 aaaaaataag caccgccatg cagtgcttat ttgtagtttt aaattgtttt attctgtttc
     3301 tagtacttct ttttcaccat tcgtttccgc gccgagtttc ttattgcgtg atttagtaac
     3361 aaaatgcagc actaaaatca ttgcggcaaa taagacgatt agaagggcaa aagacaccca
     3421 gaatccgacc cacccacgat agacaacagt agtaattaga agcatcggta caccaaatag
     3481 cgcagcggtt ccgctgccgt taactaagtc ttctgctact cctgtttcaa aatatggatc
     3541 aatttcccgt agcaaggcga cgccttcact cattgttccc gttaacatcc caaagaaacc
     3601 aaagaaagta taccatctcg tttttgggaa ataacgctta gcaacaaagt taatatagta
     3661 ataactgaca atcgtgccaa atacactcat aagcgcgagt aacacccaga aacttggatc
     3721 atttaattta ccaaagttaa ttgacataat acctgcggca atcatcacat caaaggctaa
     3781 accagcaatt ctattttgca taaagttatt aatgtattta cgcttcataa tctttttctt
     3841 gcgtaataaa tttagtactg ctttaacaat cattgtcgta ataatcgcaa aaaggaagtt
     3901 aaatccccaa ataattccgc gtatagacgc aaattttgct aaatctacaa caagaaaact
     3961 aacgagtgac ataataccaa atgcaattaa ataaatagca ataacaaacg ctacttggat
     4021 agtaaattta tcaactgatt ccacaactgg tatttcttct ggatgatcta ccaccgcata
     4081 ttgactatcc gctaaaccgc caaaagtttc tgtaacttca gcttttcttc gtgttactag
     4141 ccctttgcga gcaacgacat ttaagattac cgttccaaca acagcggcga caacaaagcc
     4201 cattgcggca atacttagac caaagtcacg accaccagca agggcattat aatccgttaa
     4261 tttttcataa acatcaccca tattaccagc ttgaccaggg ccttcaccaa aaccaagcgg
     4321 taataagaca ccagcgtaag gtgctttgcc aatagcgtaa ttaacaaaga ttaaactaac
     4381 acccaagctc aagataagtc caacaatacc ttggatcaga tatccgccaa caattaatgc
     4441 cccgcttttt agggctctac cttctttttc aactttcttt tccgcagtta ctaaccctaa
     4501 agcaataaat ccaattgcta aactatgata agttagaaaa tgcatataac tttcatcaac
     4561 aattggagta ccataaattt ctacaccaag ccattggaat aagtacttta aacctaaacc
     4621 aagaaaccca gcgattaaac ctgttggcac taagtagtta cgtaagaaag gtgttttccg
     4681 tctaatagtg ttggcaatta aaataagtgc accaacaaca ccgatttgaa agattgaaat
     4741 ccaaaacgga ttaaaatccc acgtattagc taaaaataat gtacgcatat ctcttttcct
     4801 cctttatttt tacactaacg actatagacc taaatcctgt gcagaaagac gcggaacttc
     4861 atcaggtttt gcatcaggat attgtaagat attttttaag atttgtaagg taactacaaa
     4921 ctgataggaa ctaacaattt cggtttttgg aatctttagc attttagttt gttcatggcg
     4981 cgggtaaata accaaaactt ctttttgttg catcgccatc gtatctaatt cgtcaatcgt
     5041 aaaatgctga gtaacatgtc gtttcatttt acgtttaaat tttatagtta aaccttcacg
     5101 agaaagtgtt aatgtggcag gtccaaagtg tttacggcgc tttctaaaga cttccgtaaa
     5161 attgccaggg acactaacac taaaattagg attattaatg atatgttctt gatacgctaa
     5221 attatttgct ttatctaatt caattaaatt tttgggttta tcccacttcg tgctatgcaa
     5281 atatccatgt tcatcataag ttaatatttc cccgcatttt gcgcatgaat aattattata
     5341 tgccgttttt gcagtgttca caccgtgaca aattgggcat gtaaatagta aacgatgcaa
     5401 cccttcagcg ttgcgtttac cgcgataagt atattgcgga ctttcttcat aagcattaat
     5461 atttatattc tcaatgatga gctcattaat ctcatcagcc gtcatatctt tataatcttc
     5521 gtaatataaa accttgcgaa ttttaccgcg tacatgtttt tctcttttgg tataagttgc
     5581 ccagcgtgga agcgataagt atccaccttc aaaattgaaa atcaccagcg gaatttttag
     5641 tcttaacact aactttccaa tggccgctgg catactactt agtcccccag tcatcgtcgc
     5701 atttccttca acataaacac caacactacc accttcttta acgatttgca taatgttttt
     5761 aaccgttgtt acatctgcca tgcttttagt cttaggaata ggggcaaaac agtgcgttaa
     5821 aagtcttgat ttccaaccaa gtgaaaagat taattcacta gcgacataat atatttgatg
     5881 accaaaaatt gatgtcatta aaatcggatc aaaagtaagg atatgattac tgacaattaa
     5941 acatggctca tattgcttaa tatcaatctt tgcttgttta acaccataag aacgacgaat
     6001 taatctacct agtaagaagt tagcgagatt gacataacgt tgatgacgtt ttttaacaat
     6061 ccgctgcttc ttcggttttc tattcctcac cttcaacctc cgtgataggt tttacataac
     6121 atatccggcg tttatgatta aagtaattta gatgtttcca aagccgttgg acaggtttat
     6181 tatattcaag ttgcggacca gtttcagcat actcaatccc atcggcaatc gcgcttttca
     6241 ttgattcata cattattaca ttagcaagac cactattctg caattcaggt tcaaccgcaa
     6301 caaggagtaa gtctaattgt tttgatttct taagtgcgcg taaaaagcga ataaatccaa
     6361 acgggaataa ttttccttta atctttttaa gggcaaaaac aggagacgga attgaaagcc
     6421 caaacgctaa taattcatct ttttcatcca ctactaaaat aagatatttt aagttaataa
     6481 gcgggacata ttgtcgcgct agcatatcca tgagtttttc actcattggt acataaccat
     6541 aaagatggtc gtatgctcta tttgttaacg ctaaagcttt gcggacatat ggttgaattt
     6601 gtcgttttga tttaagtctt ctaactttga cattatattt gcgttttacc atttcactaa
     6661 cgcgttcaaa acgctcatcg atttctttcg gaataataat gcggtgctca acccatacag
     6721 catcacttac aaatcctaat tcctctaaat gtttcatgta atacgggaag tgataaaatg
     6781 tggcaaacat atttgaatat tcaaaccctt ccacaagtag tccttcttta tcttggtcac
     6841 tataacctaa aggaccaaca atttcattca taccgcgtga ataaccccat tcttccactg
     6901 ctttaatgag tgcttttgtt acttcaatat catcaatcat atcgattcga ttaaatcgga
     6961 cacgtttttg atcatacttc ttattagcgt aatgactaat aataccgcaa atgcgaccaa
     7021 caattttccg gtctctatat gctagaaaca gtttaaaatc gcaccacgct gaagccggat
     7081 ttttacttgg agttaaattt gatatttcgt cttcaaaaat atacggagta aattgttttg
     7141 aatttttata taatttgacg gggaagttga tgaattgtct aagttttctt ccgccgtaca
     7201 cagggactat ttttatcata atacattcct ctacttgata attttaacat aatcaaatta
     7261 tatttgaagt aaaacatatt atgaataatg aacgaaaata aaaaactcac gattgcgtga
     7321 gctattttta aaa
//
LOCUS       DAOSTA010000045         6906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_10159_length_6906_cov_10.635820, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000045 DAOSTA010000000
VERSION     DAOSTA010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10159_length_6906_cov_10.635820"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLU08_04335"
     CDS             <1..350
                     /locus_tag="PLU08_04335"
                     /inference="COORDINATES: protein motif:HMM:NF028226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HOQ70950.1"
                     /translation="FNFYIFEYIGTLIICVIIINSSINIIKEAAYYLTDASPSKKTVE
                     SIWECILNVEGVICVDDLKVRRHMNNYYVDVEVGINKNLTLKEAHDISEKVHSQVEAN
                     FKVLHCHVHINPH"
     gene            474..1100
                     /gene="upp"
                     /locus_tag="PLU08_04340"
     CDS             474..1100
                     /gene="upp"
                     /locus_tag="PLU08_04340"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOQ70951.1"
                     /translation="MVFEIKHPLIDVKLSIMRDEKTKSKEFRESLDEIASLMSYEIFK
                     DIGTHASEDTFMTPTGVELQKIKLDNKIILVPILRAGVGMVDGIRNMIPTARIGHIGI
                     YRDEKTLEPHEYYFKLPKVDNPLVVLVDPMLATGGTAIASIDAIKARGYNNIRMATLV
                     ASPLGIERVSIAHPDVHIYVAAIDEKLNEHGYIIPGLGDAGDRIFGTR"
     gene            1106..2074
                     /locus_tag="PLU08_04345"
     CDS             1106..2074
                     /locus_tag="PLU08_04345"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009360773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOQ70952.1"
                     /translation="MSFSKDACIFSLTANKELTREVAKILGVNVSEAIVTHFADGETL
                     CEPTSSFRGKNCYVIQSTCKPVNERLMEILIFADALKRASAKSINVVMPYFGYARQDR
                     KNKPRQPITGRLVADLIHAAGIDRVVVIDLHAPQIQGFFTCLVDEMTAIPFLAKYFKD
                     KVDDDWVIVSPDHGGMARARRVAEFLNKPLAIIDKRRPKPNEATISHVIGDVKGKHCL
                     VIDDIIDTGGSSRAGAIALLEHGAKSVRIAATHGVFSPGAEVKLYEDNIFKEIVVTDS
                     IPTPEHMKGKPLTVLSLALMIAKAIEHINSGKSLTIVYEMFSNTEE"
     gene            2090..3571
                     /locus_tag="PLU08_04350"
     CDS             2090..3571
                     /locus_tag="PLU08_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HOQ70953.1"
                     /translation="MIAFDYEHYLTIQKREILKRIKLFDNKLYLEFGGKLIEDNHAAR
                     VLPGFHPSGKLEIFSSLKEQIEVIIVVNTNDIVANKIGSDNNISYQGEVERLIDEFRT
                     KDIAVCGVVFSFFSESIVTNAFIKKLARNNIKTYKHYVIEDYPHNLEKIFSEDGLGRN
                     EYVETTRPLVVVTAPGSGSGKLATCLSQLYHDNKNGIRAGYAKYETFPIWNLPLNHPL
                     NLAYEAATVDLGDFNVIDPYYLDKFNVKATNYNRDVDAFPVLKAIFEKMYGETPYESP
                     TMMGVNMVGFALGDEEAITAACKEEVIRRFYSAQKNLFLDKYTASSLDKIRMIMNKLG
                     IVETDRKCVLPALKKADETHVPCMALVLNDGKIITAKTSKLLSPAAALFLNSVKYLAN
                     INDEIPLISPNLIEPIEALKKEISAINDSVLNLNDTLISLAIQAPMNPLSDLALKQIE
                     KLKGAQAHSSVLLDKRDLQVLKQLGINVTEESVPASNKLYLRS"
     gene            3571..4110
                     /gene="hpt"
                     /locus_tag="PLU08_04355"
     CDS             3571..4110
                     /gene="hpt"
                     /locus_tag="PLU08_04355"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOQ70954.1"
                     /translation="MMMKDLEKVLVTEAEITEIINRVSARIEKDYKGKDLVMIGILKG
                     GAPFMMDLIKKINLPLIIDFIRVESYFGGTTQGELVFRKDIEVDITNKHVLIVDDIID
                     SSRTLKMVIDLFKKRNASSIEVCTLLDKPSGRVIDYTPKYIGTTIGNEFVVGYGLDYD
                     EKYRNLPVIGVLKEEVYKK"
     gene            complement(4111..5304)
                     /locus_tag="PLU08_04360"
     CDS             complement(4111..5304)
                     /locus_tag="PLU08_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOQ70955.1"
                     /translation="MRKVGTSARGIRCPIIRSGDDLAQVVVNSVLEAAKAEGFTLRDR
                     DIIGITESLLARSQGNYASIDAIASEIKAKFPGGELGVIFPILSRNRFATLLKGIARG
                     AKKIYLMLSYPRDEVGNALLSEALIEKAGVNPYTDILTVPEYRKLFGYPKHEFTGIDY
                     VELYTDIVEQESCKIELIFANHAQMILNYTKHVLTCDVHTRQDSKHKLKVAGAETIYG
                     LDDLLTTPQLGEKGFNPQYGLLGSNKATDDSIKLFPRDGQPVVEKIQQMLLTKTGKHI
                     EVLIYGDGAFKDPQGHIWELADPVVSPFFTPGLSGTPSEVKLKYLADNDFKNLTGAEL
                     TKAIAASIKAKPKDLVGTKATLGTTPRQITDLVGSLCDLVSGSGDKGTPVVLIQGYFD
                     NYTDN"
     gene            complement(5306..6721)
                     /locus_tag="PLU08_04365"
     CDS             complement(5306..6721)
                     /locus_tag="PLU08_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003336163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOQ70956.1"
                     /translation="MDYDVIIVGAGPAGYFCAYELTEKRPNLKVLLIEKGRAINTRSC
                     PILKELIDKCPINKEGFQECYPSCSITCGFGGAGAYSDGKFNITSEFGGWMQDYLERE
                     ELLDLINYVDSINLKFGASNEITDPYTNKVKEIELRAMSVGVRLLRSKVRHLGTEENF
                     KILGRIFKHLQKKVTMLFETDVVEIITENNEIRGVVTKDNEQFTAPKVLLAVGREGSK
                     WLDTILEKRGIKMDQTQVDIGVRVECNDLIMEEINKHLYEGKFVYRTSTGNTVRTFCS
                     NPSGHVVMENYNGVINVNGHSYKDEAFRSENTNFALLVSHHFAEPFNKPNEYAVKVSS
                     LANQLACGSVLVQKFGDLLLGRRTTVKRLNEGFMRPTLKEAVPGDLALCLPHKTLQSI
                     IEMIRVLDKITPGIATEHTLLYGVEAKYYSARPEMTTEFETKAIKGLYLGGDGAGLTR
                     GLAQAGACGVYIARNMLKELK"
BASE COUNT         2171 a         1200 c         1321 g         2214 t
ORIGIN      
        1 aatttaattt ttatatcttt gaatatattg ggacacttat tatttgtgtg atcattatta
       61 attcaagtat taatattatt aaagaagcag cctattactt aactgatgct tcaccaagta
      121 agaagacggt agagagcatt tgggagtgta ttttaaatgt tgaaggtgtc atatgtgttg
      181 atgatttaaa agttagacgc catatgaata actattatgt tgatgtggaa gttggtatta
      241 ataaaaattt gacattaaaa gaagcgcatg atattagtga aaaggttcat agtcaagtgg
      301 aagcaaattt taaagtttta cattgccatg tccatattaa cccccactaa ataacgatta
      361 tttgtaaaga ttattcggtt tttaacttat tacaaaattt ttaatagtta aagacccttt
      421 tttagattta tattaagtat aatatacatg tatgatttca ataggaggga cttatggttt
      481 ttgaaattaa gcacccgctg attgatgtaa agttatcaat catgcgtgat gagaaaacaa
      541 aatctaagga atttcgtgaa tcactcgatg aaattgcttc gctgatgtct tatgaaatat
      601 ttaaagacat tggtacccac gcttcagagg acacatttat gactccgaca ggcgtagagt
      661 tacaaaaaat taagttagat aataagatta ttctagttcc aatcttaaga gcgggggttg
      721 gaatggttga cggcattaga aatatgatac ctactgctag aattggtcat attggtatat
      781 accgtgatga aaaaacatta gaaccacatg aatattattt taaattacca aaagtcgata
      841 atccgctggt agtgttagtt gacccaatgt tagcaactgg tgggaccgca atagcctcga
      901 ttgatgcgat taaagctcgt ggttataaca atattcggat ggcaacactt gtggcgagcc
      961 cgcttgggat tgaacgcgtg agcatagcgc accccgatgt tcatatttat gtcgccgcaa
     1021 ttgatgaaaa attaaatgaa catggttaca tcatccccgg acttggtgat gctggtgacc
     1081 ggatttttgg aacgaggtag agattatgag ctttagtaaa gatgcttgta tattttcatt
     1141 aacagccaat aaagaattaa cgcgtgaagt agcaaagatt ttaggagtaa atgtttctga
     1201 agctattgtc acacattttg cagatgggga aacactttgt gaacctacaa gttcattccg
     1261 cgggaaaaat tgttatgtga ttcaatctac ttgtaaacca gtaaatgaac gattgatgga
     1321 aattctcatt ttcgctgatg ctttaaaaag agcttcagcc aaatcaataa atgttgtgat
     1381 gccttatttt ggctacgcta gacaagaccg taaaaataaa ccacgtcaac caattactgg
     1441 tcggcttgtc gcggatttaa ttcacgctgc tggaattgat cgtgttgtcg taattgattt
     1501 acacgccccg caaattcaag gattctttac atgtttagtc gatgaaatga ccgctattcc
     1561 ttttttagca aagtacttta aagataaagt tgatgatgat tgggtaattg tctctcctga
     1621 tcatggcgga atggcgcggg cgcgcagagt ggcagagttt ttaaataaac cactagcgat
     1681 tattgataaa aggcggccaa aaccaaatga agcgactata agtcatgtta ttggtgatgt
     1741 taaaggtaaa cattgtctag tgattgatga tattatcgat acaggtggta gttcacgcgc
     1801 aggtgcaatc gcccttttag aacatggtgc gaagtctgtt cgcattgcgg caacgcatgg
     1861 tgttttttca ccaggagcgg aagttaaatt atatgaagat aacatcttta aagaaattgt
     1921 cgttactgac tcaattccaa caccggaaca tatgaaggga aaaccgctaa ccgtcctttc
     1981 tcttgcgctg atgattgcga aagcaataga acatattaat tcaggcaaat cactcacgat
     2041 tgtctacgaa atgtttagta ataccgaaga ataaaaggag ttaaagttta tgattgcttt
     2101 tgattatgaa cattatttaa ctattcaaaa acgcgaaatt ttaaagcgga ttaaattgtt
     2161 tgataataaa ctttatctcg aatttggtgg caaacttatt gaagataatc acgctgctcg
     2221 tgttttacct gggtttcatc caagcggtaa acttgaaatt ttctcaagtt taaaagagca
     2281 aattgaagta attattgttg tcaacactaa tgacattgtc gctaataaaa ttggcagtga
     2341 taataatatc tcttatcaag gtgaagttga acggttaatc gatgaatttc gcactaaaga
     2401 tatcgccgtt tgtggtgttg tcttttcctt ttttagtgaa tcaattgtca cgaatgcttt
     2461 tatcaaaaaa ctagcgagaa ataatattaa gacatataaa cattacgtga ttgaagacta
     2521 tccgcataac ttagaaaaga tatttagtga agacgggtta ggtcgtaacg aatatgtcga
     2581 aacaacgcgt ccgcttgtgg ttgttactgc acctggttca ggaagtggga aactcgcgac
     2641 atgtttatcg caactctatc acgataataa aaacggtata agagcgggat atgcaaaata
     2701 tgaaacattc ccaatctgga atttaccgct taatcacccg cttaacttag cctatgaagc
     2761 agcgactgtt gatttaggtg actttaatgt tattgatcct tattacttag ataaatttaa
     2821 tgttaaagcg acaaattaca atcgtgatgt tgacgctttc cctgtgttaa aagctatttt
     2881 tgagaagatg tatggtgaaa cgccctatga atcaccgaca atgatgggcg tcaatatggt
     2941 tggctttgcg cttggggatg aagaagcgat taccgccgcg tgtaaagagg aagttattcg
     3001 ccgtttttat tcagcgcaaa aaaacctctt ccttgataaa tataccgcta gtagtcttga
     3061 taaaatccgg atgattatga ataaattagg cattgttgaa actgatcgta aatgtgtatt
     3121 gcctgccctt aaaaaggccg atgaaacaca tgtcccgtgt atggctttag tgcttaatga
     3181 cggcaaaatt attacagcaa aaacatcaaa acttttaagc cctgccgctg cattattctt
     3241 aaattcagtt aaatatttag cgaatattaa tgatgaaatt ccgcttattt ccccaaatct
     3301 tatcgaacca attgaagcgc tgaaaaaaga aataagtgcg attaacgatt cagtgcttaa
     3361 tttaaacgat acattgattt ctctagcaat ccaagcgccg atgaatccgt tatcggatct
     3421 tgcgcttaag caaattgaaa aattaaaagg agcacaagcg cactcttctg tgctccttga
     3481 taaacgcgat cttcaagttt taaaacaact aggaattaat gttactgaag agagtgtccc
     3541 agcatcaaat aaattatatt tgaggtcatg attatgatga aggatttaga aaaagtatta
     3601 gtgactgaag cagaaattac ggaaattatt aatcgtgttt cagcacggat cgaaaaggat
     3661 tataagggta aagatttagt gatgattggc attttaaagg gcggcgcccc gtttatgatg
     3721 gatttaatta agaaaattaa tttgccactt atcatcgact ttattcgtgt tgaatcttat
     3781 tttggtggca cgacacaagg cgaactagtc ttccgtaagg atattgaagt tgatatcact
     3841 aacaagcatg tcttaattgt tgacgatatt attgatagca gccgcacatt aaaaatggta
     3901 attgacctct ttaaaaagcg taacgctagt tcgattgaag tgtgtacact acttgataaa
     3961 ccaagtggtc gcgtaattga ttatacgcca aagtatattg ggacaactat cggtaatgaa
     4021 tttgttgttg gttacggttt agattatgat gaaaaatatc gtaacttgcc agttatcggt
     4081 gtattgaaag aagaagttta taaaaaataa ttaattatct gtgtaattat caaaataacc
     4141 ttgaattaac acaactggtg tgcctttatc accagaacca cttactaaat cacaaagtga
     4201 tccaacaagg tcagttattt gacgtggggt agtacctagc gtcgccttag tgcctacaag
     4261 gtcttttggt ttagctttaa ttgaagcggc aattgcttta gtaagttcag cgcccgttaa
     4321 atttttaaaa tcgttatcag cgagatattt tagttttact tcgctaggtg tgccgcttaa
     4381 tccaggagtg aaaaatggtg acactacagg atctgctagt tcccaaatat gtccttgtgg
     4441 atctttaaaa gcaccatcgc cataaattaa aacttcaata tgtttgcctg tttttgttag
     4501 taacatttgt tgaatttttt cgacaacagg ttgtccgtca cgcggaaaaa gtttaatcga
     4561 gtcgtctgtg gctttatttg aaccaaggag cccatattgc ggattaaatc ctttttctcc
     4621 tagttgtggc gtagtcaata aatcatccaa tccgtaaatt gtttcagctc ccgcgacctt
     4681 taatttatgt tttgaatctt gacgggtatg aacatcacac gttaagacat gttttgtgta
     4741 atttagaatc atttgggcgt gatttgcaaa aattaattca attttgcagc tttcttgctc
     4801 cacaatgtct gtatagagtt caacataatc aatgccggta aattcatgtt ttggatagcc
     4861 gaagagttta cgatattctg gcactgttaa aatatcagtg taaggattaa ccccagcttt
     4921 ttcaatgagt gcttcactta gaagcgcatt accgacttca tcgcgcgggt aagaaagcat
     4981 taaataaatt ttctttgcgc cacgcgcaat ccctttaagt aatgttgcaa aacgattacg
     5041 agaaagaatc gggaagataa ctcctaattc tccgcctggg aactttgctt taatttccga
     5101 tgcaatagcg tcaattgaag cataatttcc ttgtgagcgc gcaaggagtg actcagtgat
     5161 accgataata tcacgatcac gaagagtaaa tccttccgct tttgcggctt ctaatacact
     5221 attaacgaca acttgcgcca agtcatctcc actgcgaata atgggacaac gaataccgcg
     5281 tgcacttgta cctactttcc gcatcttact ttaattcctt taacatatta cgagcaatat
     5341 aaacgccaca tgctccagct tgcgctaagc cgcgagtaag tccagcaccg tcgccgccta
     5401 aatataagcc tttaatagct tttgtttcaa attcagtagt catttctgga cgggcactgt
     5461 aatacttggc ttcaacaccg taaagtagtg tatgctctgt cgcaatacct ggagtaattt
     5521 tatcgagcac acgaatcatt tcaataattg attgcagtgt tttatgtggt aaacaaagcg
     5581 ctaagtcgcc aggcactgcc tcttttaatg ttggccgcat aaagccttcg tttaagcgtt
     5641 tgactgtggt gcgtcttcct agtaataagt caccaaactt ttgcactaag actgaaccac
     5701 aagcgagttg atttgccagt gaagatactt taacggcata ttcatttggt ttattaaatg
     5761 gttcagcaaa gtggtgtgac acaagtagtg caaagtttgt attttctgat cgaaatgcct
     5821 catctttata agagtgccca ttaacattta tgacgccgtt ataattttcc ataacgacat
     5881 gcccacttgg atttgaacaa aaggtgcgga ctgtattacc agtagaagta cgatagacaa
     5941 atttaccttc ataaagatgt ttattgattt cttccataat taagtcatta cattcgacgc
     6001 ggacaccgat atcaacttgg gtttgatcca ttttgatgcc gcgcttttct aaaatagtgt
     6061 cgagccattt actaccttct cgtccaaccg cgagtaatac tttaggggca gtaaattgct
     6121 cgttgtcttt tgtaactaca ccacgaatct cattattttc agtgattatt tccacaacat
     6181 cagtttcaaa taacattgta actttctttt gtaaatgttt aaaaatacgg cctaatattt
     6241 taaagttttc ttcagtgcct aagtgtctta ctttactacg gagtaagcga acacccacac
     6301 tcatcgcacg gagttcaatt tctttaactt tgttcgtata gggatcagtg atttcattac
     6361 tagcgccaaa ttttaaatta atactatcaa catagttgat aaggtcgagc aattcttctc
     6421 tttctaaata atcttgcatc caaccaccaa attcactagt gatattaaat ttgccatcag
     6481 aataggctcc agcaccacca aagccacaag tgattgagca agacggataa cattcttgaa
     6541 agccttcttt attaatcgga catttatcaa ttaattcttt taaaattgga catgagcgag
     6601 tattaatggc tcgtcccttc tcaattaata aaactttgag gtttggacgt ttttcagtta
     6661 attcataagc acagaaataa cctgctggtc cagcaccgac aataatcaca tcataatcca
     6721 tagtaaaagc gtctccttaa tttatattat tttaccttga ggggtatcta gaaatttatt
     6781 ttcccttttt attataataa ataaacttat attgttagtt gaatttaata atattggtaa
     6841 cgctttatta catttgttag atgacatact tatctttaca aataaagatt aggaaacttt
     6901 taaatt
//
LOCUS       DAOSTA010000046         6496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_10954_length_6496_cov_7.782953, whole genome shotgun sequence.
ACCESSION   DAOSTA010000046 DAOSTA010000000
VERSION     DAOSTA010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10954_length_6496_cov_7.782953"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..888)
                     /locus_tag="PLU08_04370"
     CDS             complement(25..888)
                     /locus_tag="PLU08_04370"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ70957.1"
                     /translation="MIKKILAKVYVYLILFLLYLPILFLIIYSFTPAETTGVWEGFSF
                     ELYGRVFLNEKIMRALLNTLIIALSSATVATILGTLGAIGVFYMKKRPQRIMEGINEI
                     PVVNAEIVVAISLAILFRVFANFLEARGVATNSPNFVTLLIGHVVLSISFVYLNVKPK
                     LQQMDPNDYEAALDLGAKPMYAIRKVVLPAIVPGIISGFMLAFTLSLDDFIITNFLRN
                     QSFDTLSTYVQGVIVRAAIPAEMRALTTYIVLVTLAVVLVSGIKRESRSSRKAMLRAQ
                     KREVLTINKSS"
     gene            complement(881..1684)
                     /locus_tag="PLU08_04375"
     CDS             complement(881..1684)
                     /locus_tag="PLU08_04375"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ70958.1"
                     /translation="MKIRFKRSYIGIPYVLFMVLFVLLPLFFVAFYAFTNSAGRFSFE
                     PMVNFFTSSAKWRVLFVSFIFGILNTALCLVIGYPLAMILANRKYNTNAVLVMLFVMP
                     MWINFIIRSWAMRDVLVFLGIRGGEYPELATLLGLTYNYLPFTILPLYSTMLKMDKNQ
                     IEASYDLGAGPVTTFFKVIVPMTMPGIISAATMVFMPTMTSYVIADILGEGKVTLFGK
                     YIEIYFTQNIWNEGSFLALVMLALIGISVLVPKLLTRKEAKVKGVGIYD"
     gene            complement(1674..3200)
                     /locus_tag="PLU08_04380"
     CDS             complement(1674..3200)
                     /locus_tag="PLU08_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ70959.1"
                     /translation="MDKLTKHNVKKEDVIIELKDISKEYDGKTVVDDFNLYVKRGEFI
                     TFLGPSGCGKTTTLRMIAGFELPTTGTITLNGEDITKLPPYKRPVNTVFQRYALFPHL
                     DVYDNIAFGLKLKKVKDTKNGKEVMRKLTFKEIDEKVVRALKMVDLEQFEARDIATLS
                     GGQQQRVAIARAIVNEPEILLLDEPLGALDLKMRKEMQIELKDLHKRLGITFIYVTHD
                     QEEALTMSDTIVVMNDGLIQQIGTPLDIYNEPKNPFVADFIGESNIYNATMAGKLKVK
                     FLGATFDCVDDLPTNEKVDVVIRPEDIKIVAAGKGNVDGKIISRVFKGVHYEYDVQVG
                     RKSEVIIQSTIEYPENEKVGLAVAPDDIHIMKKEVTTNVYADAWIDNKNRLMIGDNAF
                     EIDITQLLKGSTVDEEGYLVGPDAKKYDLDDVDVVATIDFKDIELDDVIEEDAPMGQI
                     ISIIWLGDHYQVIVRTNDEEDFIVDSEHLWNEGDLVSIIIKPEDIKLKLKGELVPYEN
                     "
     gene            3492..4589
                     /locus_tag="PLU08_04385"
     CDS             3492..4589
                     /locus_tag="PLU08_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70960.1"
                     /translation="MKKQGLAFLLLGTFLLTGCNKPPKMEKVQIMYKYSNLTEVINIV
                     DDENQTALEQLELKITDKENFVLVSYADYTCSCWSVFRDHVLRNYINTKKIPIYVIET
                     DALGNDFKGLPIRKDLTNTPVIGIFAGGVCKYSIDYTSKSEIFIERDKFNEWMDARIK
                     DPLMTYISLEEVNTLLKGTEPFLLNWSYSICPDCVALDKQFMPGYIETLKKAPKMPYY
                     IIESKPIRDAGNWLNVKDIYGLSDKNNTVSGYATGYVPTLQVIRPDGNGATYLANNDI
                     SPMIDDMLVFQNDQVKKVDGVYKIKDSYYNGVRATRYLGEYESEVGKVVDPSIVMETE
                     YNGVLNTYFAPGSRYELHANYATKFFDHYWR"
     gene            complement(4654..>6496)
                     /locus_tag="PLU08_04390"
     CDS             complement(4654..>6496)
                     /locus_tag="PLU08_04390"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOQ70961.1"
                     /translation="TGWNREHTYPDSKISGAAKSDNHHIFADDNRTNSTRGNMPFGVV
                     AQTSTNRVKDGYGNLTDCYKDGTYFYPNEPARGEVARATMYVNMRYGYSVTGNFQSIA
                     LMLEWHLQNPVSNREIYRNNTVHTMQKNRNPFIDHPEYACRIWGNTNSETQAICGAAQ
                     QVDVTSVSVSPSSGSINLLDVNKTLQLSANVLPANATNKSVNWSTSDSSIATVDANGL
                     VTGKAVGNVTITATSVSNPSVKGTASIAVSNTSIPVTGVTISDKSFTLVKDATKTLTA
                     TVSPANASNKNVTWSSSNTSVATVSSSGVVTGVSAGNATITVTTVDGGKTDTANVTVA
                     NAPPTQSETTTYTFSTKGWKATPANWQSMYPANGFETDQNYLRGMQVLGNNTAYGNSP
                     YFDNVKKVIFGTAASSSGAGTYSAYLVTSSTSKLKDGTQIGTDVTVSKPGATIIERTF
                     ELTTPASGHVQAIIDATSNSLYLKYVTIEVATPINYQGEADLWANGFLESTSAGCSSQ
                     SQSTLSSIWPNLSNEYNALAAEVKAIIEGSTPNSAGSEVEQALARYVFIVNKYGFTAF
                     INGIPASGVHDTMHNDKQLAEPLLILFSVATLSLIPFVYKRKKRTLN"
BASE COUNT         2038 a         1297 c         1111 g         2050 t
ORIGIN      
        1 tattttttac tatgctctta tcatttaact tgatttatta atagttaaaa cctcccgttt
       61 ttgtgctctt agcatcgctt tgcgcgaaga gcgtgactcg cgtttaatcc cagacacaag
      121 cacaaccgct agtgtgacta acacaatata agtcgttagg gcgcgcatct cggcgggaat
      181 tgctgcccgc acaatcacac cttgtacata agttgaaagg gtgtcaaaag actgattacg
      241 taagaagttt gtaatgataa aatcatctaa acttaaagta aaagcaagca taaagccact
      301 gataatacca ggtacaattg cgggtaacac gactttacga atcgcgtaca tcggttttgc
      361 acctaagtca agcgctgctt cataatcatt tggatccatt tgttggagtt taggtttaac
      421 attgagatac acaaaagaaa tactaagcac aacatgccct attaaaagtg tgacaaagtt
      481 tggactattt gtcgctacac cacgtgcttc taagaaatta gcgaacaccc ggaataaaat
      541 agctaaacta atcgctacaa cgatttccgc gttaacaaca ggtatttcat taattccttc
      601 cattattctt tgcggacgct ttttcatata aaagacacca attgccccaa gagtgcctaa
      661 gattgtcgca accgttgcac tactaagcgc aataatcagt gtattaagta aggcgcgcat
      721 aatcttttca tttaaaaaga cgcgtccata aagctcaaaa ctaaagcctt cccatactcc
      781 tgttgtttcc gcgggagtaa agctataaat aatgagaaat aaaattggta agtacaaaag
      841 gaataaaatt aagtatacat atacttttgc gagaatcttc ttaatcataa ataccgaccc
      901 ccttcacttt tgcttctttt cttgttaaga gtttaggaac aaggacacta ataccgataa
      961 gcgcaagcat tacaagcgca aggaatgagc cttcattcca aatgttttgg gtgaaataaa
     1021 tttcaatata cttcccaaat aatgtcactt taccttctcc taaaatatcc gcgataacgt
     1081 aacttgtcat cgttggcata aagaccattg tcgctgcact aataatcccg ggcatcgtca
     1141 ttggtacaat tactttaaag aaagttgtta ctgggccagc gcctaagtca taacttgctt
     1201 caatttgatt tttatccatt tttagcatcg ttgaataaag cggcaagatg gtaaaaggta
     1261 aatagttata agttaaccct aaaagggtgg caagttctgg atactcaccg ccacgaatcc
     1321 caaggaacac aagtacatca cgcatcgccc atgagcgaat aatgaagtta atccacattg
     1381 gcataacaaa gagcatcact aatactgcgt tagtgttata ttttcgattc gctaaaatca
     1441 tcgctaacgg atagccgata actaagcata gtgctgtatt taatatgcca aaaataaagg
     1501 aaacgaataa aacacgccat ttcgcactac ttgtaaagaa gttcaccatt ggctcaaaac
     1561 taaagcgccc cgcactgtta gtgaaggcgt aaaatgctac aaagaaaagc ggtaataaga
     1621 cgaagagcac catgaagagc acatagggaa tcccaatgta ggaccgtttg aatctaattt
     1681 tcatacggca ctaattctcc ttttagtttt agcttaatat cttcaggttt aatgataata
     1741 cttactaagt caccttcgtt ccataagtgt tcggaatcaa cgataaagtc ttcttcgtca
     1801 ttagtacgaa cgataacttg atagtggtca ccaagccaaa taatgctgat tatttgtccc
     1861 attggcgcat cttcttcaat gacgtcatca agttcaatat ctttaaagtc aatagtggca
     1921 acgacatcaa catcatctaa atcatatttt ttagcatcag gtcccactaa ataaccttct
     1981 tcatcaacag tgctcccttt taagagctga gtaatatcga tttcaaaagc attatcaccg
     2041 atcattaagc gattcttatt atcaatccat gcatcagcgt acacatttgt tgtcacttct
     2101 tttttcataa tatgaatatc atcaggggca acagctaagc cgaccttttc attttcggga
     2161 tattcaatag tgctttgaat aataacttca cttttacggc ctacttgcac atcatattca
     2221 tagtgcacac ctttaaagac acggctaata attttcccgt caacattacc cttaccagcg
     2281 gcaacaattt taatatcttc aggacgaatc acaacatcga ctttttcatt tgtcggtaag
     2341 tcatcaacac agtcaaatgt cgcgcctaaa aacttcactt taagcttgcc cgccattgtc
     2401 gcattataaa tatttgattc accgataaag tcagcaacaa acgggttctt tggttcgtta
     2461 tatatatcta acggcgttcc aatttgttgg ataagtccgt cattcatcac gacgattgta
     2521 tcactcattg ttaaagcttc ttcttggtcg tgcgtaacat aaataaaagt aatacctaag
     2581 cgtttatgta aatcttttaa ttcaatttgc atttctttac gcatcttcag gtcaagtgcg
     2641 ccaagcggtt catcaagtaa taaaatttct ggttcattaa caatcgcgcg cgcaattgcg
     2701 acccgttgtt gttgaccacc tgagagcgtc gcgatatcac gtgcttcaaa ttgttcaaga
     2761 tcaaccattt ttaaagcgcg cacaactttt tcatcaattt ctttaaaagt aagtttacgc
     2821 attacttctt tcccgttttt tgtgtctttg acttttttaa gttttaagcc aaaagcaata
     2881 ttgtcataca catctaaatg tgggaataat gcgtagcgtt gaaataccgt atttactgga
     2941 cgtttatatg gtgggagttt ggtaatatct tctccgttaa gtgtaatcgt ccctgttgtc
     3001 ggtaattcaa aaccggcaat catgcgtaaa gtcgttgttt taccacagcc agatggtcct
     3061 aaaaatgtaa tgaattcacc acgcttcaca tacaagttaa aatcatccac tactgtcttt
     3121 ccatcatact ctttactaat atcttttaat tcaatgataa cgtcttcttt cttcacgtta
     3181 tgctttgtta atttgtccat aggtaccccc ctttcaacac aataaaatat aaggcaaatt
     3241 ttaataaaat acaagcataa ttattttaca ctttttttgg caccccgaaa aagcagattt
     3301 tattgcatta tatgataaag atttcaaata aaaaattgtc gaatttttac cccctttttt
     3361 cgtctaattt atgggtctat ttttcaccct ctttttttgt aaatttttca cccttttttc
     3421 tgcatgttgc aacgactatt aaaacttgat aaaataaact cacatataga gtgaaatcga
     3481 ggtaaaaact tatgaaaaaa cagggtttag cattcttatt attaggtact tttctattaa
     3541 caggttgcaa taaaccacca aaaatggaaa aagtccaaat catgtataaa tattcaaact
     3601 taacggaagt aataaacatt gttgatgatg aaaaccaaac agcgcttgaa cagcttgaat
     3661 taaaaatcac cgataaagaa aattttgtgt tagtaagtta cgctgattat acatgttcat
     3721 gctggagtgt gttccgtgac catgtattaa gaaactacat aaatacgaag aaaatcccaa
     3781 tttatgttat tgaaacagat gcattaggta acgactttaa aggactaccg attcgcaaag
     3841 atttaacgaa cactccggtt attggtattt ttgcaggtgg cgtttgtaaa tatagtattg
     3901 attacacatc aaaaagtgaa atctttattg aacgcgataa atttaatgaa tggatggacg
     3961 caagaattaa agacccgctg atgacttata ttagtcttga agaagttaat acactactaa
     4021 aaggcacgga accattttta cttaattgga gctatagtat ttgtccagac tgcgtggccc
     4081 ttgataaaca gtttatgccg ggttacattg aaacacttaa aaaagcgcca aagatgcctt
     4141 attacattat cgaaagtaaa ccaattcgtg acgctggtaa ttggcttaat gttaaagaca
     4201 tttatggctt aagtgataaa aataatactg tttcaggtta cgcgacaggc tatgttccta
     4261 ctttacaagt tattcgacca gatggtaatg gcgctactta tttagcgaat aatgacatta
     4321 gtccgatgat tgacgacatg ctcgtcttcc aaaatgacca agttaaaaaa gtagatggtg
     4381 tttataaaat aaaagactcc tactacaatg gtgtgagagc tacccgctat ttaggagaat
     4441 atgaaagtga agtcggaaaa gttgttgatc cttcaatcgt tatggaaact gaatataacg
     4501 gtgtgcttaa cacttatttt gctccaggat cgcgctatga acttcacgca aactacgcta
     4561 ctaaattctt cgaccactat tggcgctaac atatacttct attgttaaaa agccggggca
     4621 gacctcggct tttcttgtaa ttaaattttt atattaatta agtgttcgtt ttttgcgttt
     4681 atacacaaac gggattaagc taagcgttgc tacactaaac aaaattaaaa gcggttccgc
     4741 taattgttta tcattatgca ttgtgtcatg cacaccacta gcggggatcc cattaataaa
     4801 ggcagtaaat ccatatttat taacaataaa cacataacgc gctaaagctt gttcaacttc
     4861 actaccagca gagtttggtg ttgagccttc aataattgct ttaacttctg ctgctaatgc
     4921 gttatactca ttacttaaat ttggccaaat actacttaat gtactttgtg attggctact
     4981 acaaccagca cttgtacttt ctaagaagcc atttgcccat aaatcagcct ccccttgata
     5041 attaatgggt gtcgctactt caatcgttac atattttaag tacaaagaat tagaagtcgc
     5101 gtcaataata gcttgaacgt gacccgacgc tggtgttgtt aattcaaaag tacgctcaat
     5161 aatagtcgca cctggtttac taacggttac atcggtacca atctgtgtcc catcttttaa
     5221 ttttgaggta ctagaagtta ctagataagc actataagtt cctgccccac tacttgaagc
     5281 tgctgttcca aaaataacct ttttcacatt atcgaaatac ggactattac cataggctgt
     5341 attattacct aaaacctgca tacctcttaa atagttttga tcagtttcaa aaccattagc
     5401 aggatacata ctctgccaat tagcaggtgt agctttccaa cctttggttg aaaatgtata
     5461 agttgttgtt tcactttggg ttggcggtgc attggcgact gttacgtttg cggtatcagt
     5521 tttaccacca tcaactgttg tgacagttat tgtggcgttt cctgcgctta ctccagtaac
     5581 gaccccgcta ctagaaacag tcgcaacact tgtatttgat gatgaccatg ttacattctt
     5641 atttgatgca ttcgctggac ttactgtcgc agttagtgtt ttagtagcgt ctttaactaa
     5701 tgtgaacgat ttatcactaa tagtgacacc agtaacagga attgatgtat tactaacagc
     5761 aatagatgct gtacctttaa ctgatggatt tgataccgaa gtagcagtaa ttgtgacgtt
     5821 tcccactgct ttacccgtga ctaaaccgtt agcgtcaaca gtcgcaatac ttgagtctga
     5881 agtgctccag ttaacacttt tattagtagc gttagcgggt aaaacattcg cacttaattg
     5941 taatgtctta ttaacatcta ataagttaat gctaccactt gacggtgaca cacttacact
     6001 agtaacatcg acttgttgtg cagccccgca aatagcttgt gtttcactat tagtgttacc
     6061 ccaaatccga catgcatatt ctggatgatc aataaaagga ttacgatttt tttgcattgt
     6121 atgaacggta ttattgcgat atatttcgcg atttgataca ggattttgta aatgccactc
     6181 tagcataagt gcaatacttt ggaagttacc agtcacacta taaccataac gcatattaac
     6241 atacatcgta gcgcgcgcta cttcaccacg agcgggttcg tttggataaa aataagtacc
     6301 atctttatag caatctgtca aatttccata accgtcttta acccgatttg tgctagtttg
     6361 agcaacaaca ccaaatggca tgttgccacg agtactattt gtgcgattgt catcagcgaa
     6421 aatatgatgg ttgtccgatt tggcagcacc cgatattttg gagtcaggat aagtatgttc
     6481 tctattccaa cctgta
//
LOCUS       DAOSTA010000047         6488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_10974_length_6488_cov_8.989119, whole genome shotgun sequence.
ACCESSION   DAOSTA010000047 DAOSTA010000000
VERSION     DAOSTA010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10974_length_6488_cov_8.989119"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1778
                     /locus_tag="PLU08_04395"
     CDS             60..1778
                     /locus_tag="PLU08_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOQ70962.1"
                     /translation="MKRRKLLLIPLIALLLGGCGDSSSSAVVTSEAPVTSVSEEVTPT
                     GITISGPGSLAVDESAYFNVTVKPEGAPQSVTWSTSDATKATVTESGEVTGVSEGNVF
                     VIATSTVKAEVTAQVPLVITPKVIVAPDPTEILITSVDDLTTVSVDKTLKLNATVLPV
                     DASQEVNWSSSDETKATVDATGLVTGVALGTVNITATSAVKESVKKVFTLEVVEDAIT
                     QNWEEMAYSSHADFVNKNTEDGTPFKVRGVVTYAAPVTAEGTVEYFIQNGTEGYYIYK
                     QDNDLFPVTVGNSYTVGGYFKNHFGTFELVDVEYFVPHDPALTYEVVDISDKDVLSLD
                     IMAEHQASFVEIKAAVVSVLPTNYTKAYNVKVNVGDKEFTLRVDPALMSADEFNAITA
                     LFQGTAPGSAIDVVGVMSAFGYGTPANQMKIVRSTDITAAGMTDKQATEVAAASLEIV
                     ESIPLNVNNVTLPTTLEGYEGVTVEWTSSNAALNTETGAVTHPVSTLDLTLTATVKKG
                     TDQTVRSWTVTVFGTNLESLSFLHTLDLEDADPSGSYGCSNTKPKYTVEATNDMVDLG
                     DPVTKW"
     gene            1827..2159
                     /locus_tag="PLU08_04400"
     CDS             1827..2159
                     /locus_tag="PLU08_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70963.1"
                     /translation="MYAIRTQSNNDQARSGRIELQDNVYDFNMLEFDAAIYDANRKGM
                     VLMFEVSTDDGVTWTQLEREYTINHFDLVKYRVRTGTTGNVRVAIVLKAGTGQRINID
                     NIRLIKEA"
     gene            2164..3681
                     /locus_tag="PLU08_04405"
     CDS             2164..3681
                     /locus_tag="PLU08_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOQ70964.1"
                     /translation="MRRLATIGLLVILALTGCGKRTSSEVSLTTSAPSLSDEPSIATP
                     LSIQVFKNDIRAYDVTVDVSHTINLEARVLPLDAPQEVVWSVVNSELATVSATGAVTG
                     VAPGETMINVHALNYPDVKTSLFLTVNKVAEQTGVGSGTSADDPLFKGYEGDGPLEVY
                     FLETYKIYADSLYIKKGNIDIVIDGGWKCDGELNRDFLNEKVTDGRIDMLIASHGDGD
                     HTQGIPYLVRDIPHISSFLDYGRAGGESSGYKQLIRTFGENEGSPYYTALDSVNKVNG
                     ATNRYYFTSEFYVDVLNTGAYSDTSSGNANSLALMFCYKDFKFFTAGDLTTGSETSLL
                     RNETLPQVSLYKASHHGSHGSNSQALMDTLNPYTVAIAATRAGIYNQDPAPPSPTNTY
                     NLDGTKGHPAAAAIERIYKIPRISLNLNVFWNAPNGTMKFTTYGGANDLVMNGSTPLR
                     HYYDLTLTNGEPVWNEELQDFENKVTGEENTKFHETKIFTFRGYTEYLPAWVTAA"
     gene            complement(3753..5459)
                     /locus_tag="PLU08_04410"
     CDS             complement(3753..5459)
                     /locus_tag="PLU08_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HOQ70965.1"
                     /translation="MKTFHFNDDYALINFDGINVNDALLILNSKSFEKVFRSFHRSLK
                     EKESVLLVSLGKLTATDLLEFYSLLFKYELSTVLRRMPKVTKDHVLPIYELTESFYDY
                     WRKYERFGLLNAFKEDGTQTKVDDLLKVSNDFNYQIITIYRTISQKLLGQTFNVYRQL
                     PAGISANMLYTYHSFDKSLKEYKLLNNIPVATKIVINPPFIINSKSNVRKGVFTPLTY
                     SYNPITHLKINKNNFIAFPLWVGPLLAYVYIHIDLLHHGIALSNLFEIADYQKVKDLK
                     PNLIYIYGVEDSLYDSKFYHDTVNDIYLGFVYKSAENDYFGYLKKMLLTLHNLYMINH
                     DNLPVHGAMVEITLKSNIKKRVVIVGDSGAGKSETLEALRQIASDEIKDIKVVFDDMG
                     TLSTNEDGKVLASGTETGAFVRLDDLEQGYAYEVMDRALFLNPSQVNARVVIPLSRYS
                     FILEKYPIDLFLYANNYEDKDGIKVFDKPEEALAVFRKGMRRAKGTTSEVGMTASYFA
                     NPFGPMQYRAKTDRLLNKYFKALSDNKVILGELYTKLALPEEEIRGPANAALALFKLL
                     QG"
     gene            5563..>6488
                     /locus_tag="PLU08_04415"
     CDS             5563..>6488
                     /locus_tag="PLU08_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ70966.1"
                     /translation="MLFSPEIKESPTLLLGEKARQKKEKGEKVISLAVGEPDFPTPEY
                     IIDATKKALDEGYTKYSTPQGLLELRTLIAKEYKPYKAEEVIIFPGAKAAIHAALTSI
                     LEPGDEVIIVEPYYVSYPAMIKLAEPKAKIVKIALNKDFTFPLEKLEEKITKKTKLLI
                     LNYPHNPTGQILSFQETMKIVNLVFRNKIYLLSDEIYDKLNFSGKAFHSFAEFPLIKD
                     QLVVINGFSKTYAMTGFRLGYALANKELINKMNLVNQNINTNTATFIQKGAVAALTNK
                     PMFLIPYNNELKERALYLHEEINKLPLFSGVLP"
BASE COUNT         2108 a         1195 c         1226 g         1959 t
ORIGIN      
        1 aaaggagatg tattacctaa tcgaagtacc ggggcatgat tagataatac aagatgttta
       61 tgaaacgaag aaaattatta ttgatcccac ttattgcctt acttttaggt ggttgtggtg
      121 atagtagcag tagtgcagtc gtcactagtg aagcgcccgt aactagtgtt agtgaagaag
      181 taactcctac tggtattaca atcagtgggc cgggctcgtt agcggttgat gaatctgctt
      241 actttaatgt tactgttaaa ccagaaggcg caccgcaaag tgtcacttgg tcgacaagcg
      301 atgccactaa agcaacagtc actgaaagcg gagaagtaac tggtgttagt gaaggtaatg
      361 tctttgtcat tgctacctcc actgttaaag ccgaagttac tgcccaagta ccacttgtga
      421 ttacgccaaa agtaatcgtc gctcctgatc caactgaaat tcttattact tcagttgatg
      481 acttaacaac tgtcagtgtt gataaaacac ttaaattaaa tgccaccgtt ttaccggttg
      541 acgctagcca agaagttaat tggtctagta gtgacgaaac aaaagcaacc gtggatgcca
      601 ctggtttagt cactggtgtg gcgcttggca cagtaaatat tacggcaaca agtgctgtta
      661 aagaaagtgt taaaaaagtc tttacgcttg aagtcgtcga agacgcaatt acccaaaact
      721 gggaagaaat ggcgtatagc tcacacgctg atttcgtcaa taaaaatacg gaagacggca
      781 ctccttttaa agtccgtggt gttgtcactt acgccgctcc agtaacagcc gaaggtactg
      841 ttgaatactt tatccaaaat ggtactgaag gatactatat ctacaaacaa gataatgact
      901 tattccctgt tactgttggt aattcttaca ccgtcggtgg ttattttaaa aaccactttg
      961 gcacatttga actcgttgat gttgaatact ttgtgccaca tgacccagct ttaacttatg
     1021 aagttgtgga tattagtgat aaagatgtct taagtttaga cattatggcg gaacaccaag
     1081 cttcatttgt agaaattaaa gctgctgttg ttagtgtgct ccccacaaac tatactaaag
     1141 catataatgt caaagttaat gttggtgata aagaatttac acttcgtgtt gatccggcgt
     1201 taatgagtgc tgacgaattt aatgcgatta cggctttgtt ccaaggcacc gctcctggtt
     1261 cagcgattga tgttgttggt gttatgagtg cgtttggcta tggcactccc gctaaccaaa
     1321 tgaagattgt acgaagcact gatattacgg cagcagggat gactgataag caagccacag
     1381 aagtcgcggc cgcaagtcta gaaatcgttg agagcatccc gcttaatgtt aataatgtta
     1441 cactacctac tactttagaa ggctacgaag gtgtaactgt tgaatggact tcaagtaacg
     1501 cagcacttaa cacggaaaca ggtgctgtta cgcatccagt ttcaacactt gacctcacat
     1561 taacggcaac agtcaaaaaa ggaactgacc agacagttcg ctcttggact gtaaccgtct
     1621 ttggtacaaa cttagaaagc ctttcattct tacatacatt agacttagaa gacgctgatc
     1681 ctagcggtag ttatggttgt agtaatacaa aaccaaaata tactgtagaa gccacaaatg
     1741 acatggttga tttaggcgac cctgttacta aatggtaact taaaaacgcc ttaattggcg
     1801 gcgataataa tgataagcga actggaatgt acgctattcg tacccaaagt aataatgacc
     1861 aagctcgttc tggacggatt gagttacaag ataatgtcta tgacttcaat atgcttgaat
     1921 ttgacgctgc aatttatgat gctaatcgta aaggcatggt cttgatgttt gaagtttcaa
     1981 cggatgatgg tgtcacttgg acgcagcttg aacgcgaata cacaatcaat cactttgatt
     2041 tagttaagta tcgtgtacgt actggtacaa caggtaatgt tcgtgttgcc atcgtcttaa
     2101 aagctggtac tggtcaacgc atcaacattg ataatattcg cttaatcaaa gaggcttaag
     2161 cttatgagac gtttagccac aattggttta ttagtaatct tagcgcttac cgggtgcggt
     2221 aagcgcacct cttcggaggt gtcactcaca acaagtgcac cttcattaag tgatgagcct
     2281 tcaatagcca cacctttaag cattcaagtt tttaaaaatg atattagagc atatgatgtc
     2341 acagttgatg ttagtcacac gattaatttg gaggctagag tattaccgct agatgccccg
     2401 caagaagttg tgtggagtgt cgttaatagt gaactagcaa ccgtgagcgc tacaggtgcg
     2461 gtaactggtg tagcgcctgg cgaaacaatg attaatgtgc acgcgcttaa ttatcctgat
     2521 gttaaaacta gtttgttttt aactgttaat aaagttgcag agcaaacagg tgtcggaagt
     2581 ggtacaagcg ctgatgatcc actttttaag ggctatgaag gcgacggccc acttgaagtt
     2641 tatttccttg aaacatataa aatatacgct gattcgcttt atattaaaaa aggcaacatt
     2701 gatattgtga ttgatggtgg ctggaagtgt gacggtgaac ttaatcgtga cttcttaaat
     2761 gaaaaggtaa ctgacggaag gattgatatg cttattgcta gtcatgggga cggtgaccat
     2821 acacaaggta ttccttatct tgtgcgcgac ataccgcata ttagtagctt ccttgattac
     2881 ggcagagctg gcggcgaaag cagtggctat aaacagttaa tccgcacttt tggtgaaaac
     2941 gaaggttcgc catactacac tgctctagat tccgtgaata aagtaaacgg tgcgactaat
     3001 cgctattact tcaccagtga attttatgtt gatgttttaa ataccggagc atatagtgac
     3061 actagcagcg gtaacgctaa tagtctcgca ctaatgttct gttataaaga ctttaaattc
     3121 tttactgctg gtgacttaac cactggtagt gaaactagtc ttttacgcaa tgaaacatta
     3181 ccacaagtaa gtttatacaa agctagtcac cacgggagtc atggtagtaa cagtcaagcc
     3241 ttaatggata cattaaaccc ctatactgtt gcgattgccg cgacaagagc cggtatatac
     3301 aatcaagatc ctgctccacc aagtcctact aatacatata atttagatgg cactaagggt
     3361 caccccgcag cagcagcgat tgaacgcatt tataaaattc cgcgcatttc tttaaactta
     3421 aatgtctttt ggaatgctcc taacggaaca atgaaattta caacatatgg cggcgctaat
     3481 gacttagtga tgaatggtag taccccactt cgccattatt atgacttaac actcacaaac
     3541 ggtgaacctg tatggaacga agaactccaa gactttgaaa ataaagtaac aggtgaagaa
     3601 aacactaagt tccatgaaac taaaatcttt acatttagag gttataccga gtacttacca
     3661 gcgtgggtta ccgctgctta aataattaaa atacagcaaa aaagtcagga gcgattcctg
     3721 acttttcttt atgaatagtt aaaacgatct tactaccctt gtagtaattt aaatagcgct
     3781 agcgcagcgt tcgcgggtcc tctaatttcc tcttcaggaa gcgctaattt agtgtagagt
     3841 tcccctaaaa ttactttatt gtcactaagc gctttaaagt atttattaag taatcgatct
     3901 gtcttagcgc gatattgcat tggaccaaac ggattagcga agtaagaagc agtcattccg
     3961 acttcactcg tagtgccttt tgcccttcgc attcctttac gaaaaacagc gagtgcttct
     4021 tcaggtttat caaatacttt aatcccgtct ttatcttcat aattattcgc atataagaag
     4081 agatcaatcg gatacttttc taaaataaat gagtatctac ttaaaggaat aacgactcta
     4141 gcgttaactt gcgaaggatt aagaaatagt gctcgatcca ttacttcata agcatagcct
     4201 tgttctaagt catctagtct aacaaaagca cctgtttctg tcccacttgc aagtacttta
     4261 ccatcttcgt tagtgcttag tgtccccata tcatcaaaca cgactttaat gtctttaatt
     4321 tcgtcacttg caatttgccg taatgcttct aaagtttcac ttttcccagc accactatca
     4381 ccaacaataa ctactctttt tttaatgttt gattttaaag taatctctac cattgcacca
     4441 tgtactggta aattatcatg attaatcata taaagattat gtaatgtaag taacatcttt
     4501 ttaagataac caaaataatc attttcagca cttttataga caaaaccgag ataaatgtcg
     4561 tttactgtat cgtgataaaa tttagagtca tataacgaat cttcgacacc ataaatataa
     4621 attaagtttg gttttaagtc tttgactttt tgataatccg cgatttcaaa aagattagat
     4681 aaagcgatac cgtggtgtaa taaatcaata tggatgtaga cgtacgctag aagtggtccc
     4741 acccataacg ggaaagcaat aaagttattt ttatttattt ttaagtgagt aattggatta
     4801 taagaataag ttaaaggtgt aaagactcct tttctcacat tactctttga attaatgata
     4861 aagggtgggt taataacgat tttcgtcgct acagggatat tattaagcag tttatattct
     4921 tttaaacttt tatcaaaaga gtgatatgtg tataacatat tagcgcttat accagcgggt
     4981 aactgacgat atacattaaa cgtttgtcct aataactttt gactaatagt cctgtatatc
     5041 gtaataattt gatagttaaa gtcattactt acttttaaga gatcatcgac tttagtttga
     5101 gtaccatctt ccttaaaagc attaagtaac ccaaatcttt catacttccg ccagtagtca
     5161 taaaagcttt cggttaattc atatattggt aaaacatgat ctttcgtgac tttaggcatc
     5221 cgtcttaaca cagtactaag ttcatattta aaaagaaggc tatagaactc taataaatca
     5281 gtcgcggtta gtttacctaa actaacaagt aaaacactct ctttttcttt taaagagcgg
     5341 tgaaagcttc taaatacttt ttcaaaactt ttggagttta atattaagag cgcatcatta
     5401 acattaatac catcaaagtt aattagtgca tagtcatcat taaagtgaaa agtcttcatc
     5461 aatattcccc ttctcttaat tatataatgt accataagcg accatttttt atcaaaaaat
     5521 gagcaaaata tggtaaaatt gataagaaaa gaggtatttt ttatgttatt tagtccggaa
     5581 ataaaagaaa gtccgacact tttacttggt gaaaaagcgc ggcaaaaaaa agaaaaaggt
     5641 gaaaaagtta tttcactagc ggtaggcgaa cctgattttc ctacgcctga atatataatt
     5701 gacgcaacaa agaaagcact tgatgaagga tatacgaaat attccactcc gcaagggtta
     5761 ttagaattac gaactttaat tgctaaagaa tataaacctt acaaagctga agaagtaatt
     5821 atctttcccg gcgctaaagc ggcaattcac gcggcattaa cgagtattct tgaaccaggt
     5881 gacgaagtta ttatcgttga accttattac gttagttatc ccgcaatgat taaactagcg
     5941 gaacctaaag ctaagatcgt taaaatagca cttaataaag attttacttt cccactagaa
     6001 aagttagaag aaaagattac taaaaaaaca aaattattaa ttcttaatta tccgcataac
     6061 cccactggtc aaatcttatc attccaagaa acaatgaaaa ttgttaattt agtatttagg
     6121 aataagatat atttactaag tgacgaaata tatgacaaac ttaatttcag cggtaaagcc
     6181 ttccactctt ttgcagaatt cccgcttatt aaagaccaac tagtggtcat caatggcttt
     6241 agtaaaacat acgctatgac agggtttaga ttaggatatg ctctcgctaa taaggaatta
     6301 ataaataaga tgaatttagt aaatcaaaat attaacacaa ataccgcaac ctttattcaa
     6361 aaaggcgcag tagcggcact aactaataaa ccaatgtttc taattccata taacaatgaa
     6421 ttaaaagaaa gagcacttta tttacacgaa gaaataaata aattaccact ctttagtggt
     6481 gtattacc
//
LOCUS       DAOSTA010000048         6421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_11108_length_6421_cov_8.841816, whole genome shotgun sequence.
ACCESSION   DAOSTA010000048 DAOSTA010000000
VERSION     DAOSTA010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11108_length_6421_cov_8.841816"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1252)
                     /locus_tag="PLU08_04420"
     CDS             complement(44..1252)
                     /locus_tag="PLU08_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOQ70967.1"
                     /translation="MHINKITVVGLGYVGLAQALLFNEDYNVVGYDTDTKVVASLNRG
                     ILHIKDEDFARALARKKLMATSDITYAFQNSDLVIIATPTHFNEEKQTFDLSSIDETL
                     QKIIASNRSPLVIIKSTVNVGATRYFMEKYPSLKIFFVPEFLREGHALFDNYYPARII
                     VGYDKNDTNHRQIAIQIANLFKKYTKSIDDVPLLITGTLEAEAIKLFANSYLALRVSF
                     FNEIDTFALSKDLEVKDIIEGVGLDSRIGTHYNNPSFGYGGYCLPKDSKQLKAQFNLI
                     PEELISATVNANSVRKEFIANYIADIVKKSNNKDYVVGIYRLTMKKDADNMRFSSIID
                     VVRLLKEQNIKMIIYEPLLNETTYQGITLINNLETFKKESSLIVTNRMHSDLKDVTNK
                     IFTRDVYYRD"
     gene            1344..1727
                     /locus_tag="PLU08_04425"
     CDS             1344..1727
                     /locus_tag="PLU08_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HOQ70968.1"
                     /translation="MKNVYTNMCMVYSGDKILVLNRVKKDWPGLTFPGGHVEDGELFS
                     ESVIREMKEETGLTIRNPEYVGQIVWPLPEQGINDVALLYRTNEFAGELLESVEGHVF
                     FIKKSELGNYQHSLDFDKVLELMFK"
     gene            complement(1733..2608)
                     /locus_tag="PLU08_04430"
     CDS             complement(1733..2608)
                     /locus_tag="PLU08_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70969.1"
                     /translation="MAIMSQLFNSYRFFEGIADMLGGIDFISYIFGFLSQFPIVVDII
                     IASAIIAALLRGIFKRFWKVVWRGVIFVILLIVLFATAEKLAPYIGSIPIPLRGTVNG
                     NAVEYANLGQIIYGVSIEAGNSETYAFEFTKLALKNLVVFFGMPALSLITPAISAITY
                     PLFSLLLPRKLKDAKLILPRIAISLGLTFIALVIFMAPAANFVPAMSAVKETMSEGTL
                     LYKFLNPEVINFLKLFTPQKSVMLKIVDIGNISGKIKIFEEFTVDGVVIQLKDAVEAL
                     LEALKTIPYEAPAAG"
     gene            complement(2715..4010)
                     /gene="eno"
                     /locus_tag="PLU08_04435"
     CDS             complement(2715..4010)
                     /gene="eno"
                     /locus_tag="PLU08_04435"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOQ70970.1"
                     /translation="MPTIIDVYAREVIDSRGNPTIEVDVTTESGAFGRAIVPSGASTG
                     EREALELRDGDKSRYLGKGVLTAVENVNKIIAPEIIGMDADDQVGIDKVLLELDGTPF
                     KKKLGANALLGVSLAAARAAADYFGVSLYNYIGGVNAKMLPVPMMNVINGGAHADSSV
                     DFQEYMIMPVGAPSLREALRWGAEVFHNLKKVLKAKGHITAVGDEGGFAPNLSSNEEP
                     LQVIVEAIKKAGYVPGKDFVLALDVAASEFFDEKTKKYVLKKSGGAVKTSEEMVTWYE
                     ELCEKYPIISIEDGLAEGDWDGWKLLTDRLSHKIQIVGDDLFVTNPAILKEGIEKGIA
                     NSILIKLNQIGTLTETLDAMQMAERAGYTCVVSHRSGESEDTTIADLAVALNAGQIKT
                     GSMSRTDRIAKYNQLIRIEDDLGERAIYPGLSAFYNLRK"
     gene            4167..4571
                     /locus_tag="PLU08_04440"
     CDS             4167..4571
                     /locus_tag="PLU08_04440"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOQ70971.1"
                     /translation="MEQKEIKNVVAALIFYDGKLFAASRSEKDERYGKYEFPGGKIEL
                     EENPSSAIVREIKEELMMDISVKDLFMHVSHEYPWFKLEMDVFICEVTNPQFTLLVHK
                     DGGFFTKEEILKLPFLGADLQVLAEISKRLDF"
     gene            complement(4568..5158)
                     /gene="rdgB"
                     /locus_tag="PLU08_04445"
     CDS             complement(4568..5158)
                     /gene="rdgB"
                     /locus_tag="PLU08_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOQ70972.1"
                     /translation="MKKQIVIATNNANKLKEFKSLFANTPYEVLSLADINFTGVIKED
                     GESFSDNAVIKAMTIHKLTNLPVIADDSGLSVNALEGFPGVNSARFMEGSPYTEKNAA
                     IIKMLEGHSDKSAKFICAIALVGLDSFPQVFIGEVKGTIINEARGEEGFGYDPIFFHP
                     NSQKTFAEMTLEEKDEISHRGRATALLFEYLETHYF"
     gene            complement(5163..6227)
                     /locus_tag="PLU08_04450"
     CDS             complement(5163..6227)
                     /locus_tag="PLU08_04450"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HOQ70973.1"
                     /translation="MSNLVGYSIVALFVIVMGLLLLLKVYLQTYHPGKYWYIERPIKY
                     LMILGPMFFLFAIGERWHFGENFLPSKNPDDLAWGPFHLGWLFAMVIAIIVVSSGVKA
                     DKANTKRYVFGQLNKIDFTVFQFGVLLFGIELYKQLIFLNLYEGLANYHWYGFPLQFC
                     SIPIFLYPLTPFIKNEKIKEAIYSFISIFNLIGGLAVMILATGVYTLQVSISIHTMIW
                     HGVMVVVAFYLINAYKIGTKWRHYLGAVTVLFCLIVLAQLTNILFHYIGMKFPGPGDF
                     DGFFISPWIDRRNMPILGDIRANMIAGGVPTLIIALVFPHIYFVIFSLTGLLIYYLFH
                     FIWKDVEKNKKEKALKTNTL"
BASE COUNT         2124 a         1164 c         1075 g         2058 t
ORIGIN      
        1 tttattatta aacttattaa cgaaaagaaa taatgaagat atattaatcg cgataataga
       61 catcgcgggt gaatattttg ttagtgacat cttttaagtc actatgcata cggtttgtga
      121 caattagtga actttctttt ttaaatgttt ctaaattatt aatgagcgta ataccttgat
      181 aagttgtttc gtttaaaagc ggttcataaa taatcatttt aatgttttgc tcttttagta
      241 atctaactac atcaatgatt gatgaaaagc gcatattatc agcatctttt ttcattgtta
      301 agcgataaat accaacgaca taatctttat tattgctctt ttttacaatg tcggcgatat
      361 aattagcgat aaactcttta cgcacactat ttgcgttaac agtcgcactg attaattctt
      421 cgggaattag attaaattgt gcttttagtt gtttactatc tttcggtaaa caataaccac
      481 cataaccaaa tgaaggatta ttgtaatgtg tcccaatgcg cgaatctaag ccaacacctt
      541 caataatatc tttaacttct aagtctttag ataacgcaaa tgtatcaatt tcattaaaga
      601 aagatacgcg gagcgctaaa taactattag cgaataattt aattgcttca gcttctaaag
      661 tgcccgtaat taaaagcggg acatcatcaa tagatttagt gtatttttta aagagattag
      721 caatttgaat agcaatttgc cggtggtttg tgtcattttt atcgtagccg acaataattc
      781 gcgctggata ataattatca aaaagggcat gaccttcgcg taaaaattca gggacaaaaa
      841 agatttttaa tgatggatac ttttccataa aataccgagt cgcgccaaca ttaaccgttg
      901 atttaataat aacaagcgga ctcctattac ttgcgataat tttctgtaaa gtttcatcaa
      961 ttgatgagag atcaaatgtt tgtttttctt cattaaaatg ggtaggtgtc gcaataataa
     1021 caagatcact attttggaat gcataagtta tgtcacttgt tgccattagt tttttacgag
     1081 caagcgctcg cgcaaagtct tcgtctttaa tatgtaaaat accgcgattc aaactagcga
     1141 caactttcgt atctgtgtca taaccaacaa cattatagtc ctcattaaaa agtaaggctt
     1201 gcgctaagcc gacgtatccc aatccaacaa ctgtaatttt attaatatgc atatgcttcc
     1261 cttaaattat aacatttatc aaattattca ctaatgcaga gtaccttttt tctaaaactt
     1321 ttgaattatg gtacgatttt aatatgaaaa atgtttacac aaatatgtgc atggtttatt
     1381 caggtgataa gattttagtg cttaaccgtg ttaaaaaaga ttggccgggc cttacttttc
     1441 caggtggcca tgttgaagat ggtgaattat ttagtgaaag cgtaattcgt gaaatgaaag
     1501 aagaaacagg tttaacaatt cgtaatccgg aatacgttgg tcaaatcgtc tggccgcttc
     1561 ctgagcaagg gataaatgat gttgcccttt tatatcgcac aaatgaattt gctggtgaac
     1621 ttttagaaag tgttgaaggt cacgtctttt tcattaaaaa aagtgagctt ggtaattatc
     1681 aacactcact tgattttgac aaagttctag aactaatgtt taaataataa gattatcctg
     1741 ctgctggagc ttcgtaaggt atagttttaa gtgcttcaag taaggcttca acggcatctt
     1801 ttagttgaat aacaacccca tcaacagtaa attcttcaaa gattttgatc ttgccagaaa
     1861 tatttccaat atcaacaatt tttaacatta ctgatttttg tggtgtaaat aattttaaga
     1921 aattaataac ttcgggattt aagaatttgt agagcaatgt gccttcactc attgtttctt
     1981 tgacagcact catcgctggt acaaagtttg cggcgggagc cataaagata acaagggcga
     2041 taaaggttaa gccaaggcta attgcaatac gcggaaggat gagtttagcg tcttttaact
     2101 tacgtggaag taataaacta aataacggat aggtaattgc tgaaattgcc ggtgttatga
     2161 gtgataaagc gggcatccca aagaaaacaa caagattttt aagcgctagt tttgtaaatt
     2221 caaaagcata ggtttcacta ttacctgctt caattgaaac accataaatt atttgtccta
     2281 agtttgcata ctcaacagcg ttgccattta ctgtcccacg gagcgggata ggaatactac
     2341 caatataagg tgcaagtttt tctgctgttg caaataagac gattaaaaga ataacaaaga
     2401 tgacaccacg ccagacgact ttccaaaatc ttttgaaaat accgcgtagc agcgcagcga
     2461 taatcgcact tgcaatgatg atatcaacga caattgggaa ctgggataaa aaaccaaaaa
     2521 tatatgaaat aaaatctatt ccaccaagca tatcggcaat accttcaaaa aagcgataag
     2581 aattaaagag ttgactcatt attgccataa gattacctcc tgcttaaatt atacataatt
     2641 aataattaaa aaaatataga tttataaaaa aagagggttt cccctctttt aatgtgacaa
     2701 gtaatattac gcaattattt gcgtaaatta tagaaagcac ttaaaccagg atagattgct
     2761 ctttcaccaa gatcgtcttc aatgcgaatt aattggttgt atttagcaat ccggtcagta
     2821 cggctcatcg aaccagtttt aatttgacca gcgtttaaag cgactgctaa gtcggcaata
     2881 gttgtatctt cactttcacc agaacggtgg ctaacaacac aagtgtaccc agcgcgttcc
     2941 gccatttgca tagcgtctag tgtttcagtt aaggtaccaa tttgatttaa tttaataaga
     3001 atggagtttg cgatgccttt ttcaatacct tctttaagaa ttgctgggtt tgtaacgaat
     3061 aagtcgtcac caacaatttg aatcttatgg cttaaacggt cagttaaaag tttccagcca
     3121 tcccagtcgc cttctgctaa accgtcttca atcgaaataa ttgggtactt ttcacataat
     3181 tcttcgtacc atgtaaccat ttcttcactt gttttaacgg ctccgcctga tttcttaagc
     3241 acatatttct ttgttttttc atcaaagaat tcactcgccg cgacgtcaag cgctagaaca
     3301 aagtctttac ctggaacgta tcctgctttt ttaatagctt caacgattac ttgtaaaggt
     3361 tcttcgttac tacttaagtt tggtgcaaag ccgccttcat ccccaactgc ggtaatatga
     3421 cctttggcct ttaaaacttt ctttaagtta tggaaaactt cagcgcccca gcggagtgct
     3481 tcgcgtaagc ttggtgcccc aactggcata atcatatatt cttggaaatc aacactgcta
     3541 tcagcatgtg cgccaccgtt aataacattc atcattggga ctggtaacat tttggcatta
     3601 acaccaccaa tgtagttata taaacttaca ccgaaataat ctgcagcggc acgagcagca
     3661 gctaaactaa caccaagtaa tgcgttagcg cctaatttct ttttaaatgg ggtaccatca
     3721 agttctaata aaactttatc aattcccact tggtcatcgg catccattcc gataatttct
     3781 ggagcaataa ttttgttaac attttcaaca gcggttaaca ctcctttgcc gagatagcga
     3841 cttttatcac catcacgtaa ttctaatgct tcgcgttcac ctgtactagc gccacttgga
     3901 acaattgcac gtccaaaagc accagattca gtcgtaacat caacttcgat cgttgggtta
     3961 ccacgtgagt cgattacttc acgagcatac acatcaataa ttgttggcat gtatttttcc
     4021 tcctctttgt aaatagccac atatattata atattttttc tgcttgcttt gtagtaaaaa
     4081 gaactaaaga catcgtttta tcagtaaata tatcccgata tcaatttaca gcgtaaaaat
     4141 atgttacttt gttatactaa taaagtatgg aacaaaaaga aattaaaaat gttgtggcgg
     4201 cccttatctt ctatgatggt aaacttttcg ctgcttcacg aagtgaaaaa gatgaacgct
     4261 acggtaaata tgaatttccc ggtggaaaaa ttgaactaga agaaaatcca agttctgcta
     4321 ttgtacgcga aattaaagaa gaattaatga tggatataag tgtcaaagac ttatttatgc
     4381 atgtaagtca tgagtatcca tggtttaaac ttgaaatgga tgtttttatt tgtgaagtta
     4441 ctaacccgca atttacctta ttagtgcata aagatggtgg tttttttaca aaagaagaaa
     4501 tattaaaatt gcccttctta ggggctgatc ttcaagtgct tgctgaaatt agtaagcgtt
     4561 tagactttta aaaataatgc gtttctaaat attcaaatag taatgccgtc gcccttccgc
     4621 gatggctaat ttcatctttt tcttcaagcg tcatttcggc aaatgttttt tgactgtttg
     4681 gatgaaagaa tatcgggtca taaccaaatc cttcttcgcc acgcgcttcg tttattatag
     4741 tgcctttaac ttcgccaata aacacttgcg ggaaactgtc aaggccgaca agggcaatag
     4801 cacatataaa ctttgcgctt ttatcactgt ggccttcaag cattttaata attgcagcgt
     4861 ttttctcagt ataaggtgag ccttccataa accgcgcaga attaacacca ggaaaacctt
     4921 ctaaagcgtt aacacttagt ccactatcat cagcgataac tggtaaattc gtcaatttat
     4981 gaatagtcat agcttttata accgcattat cactaaacga ttcgccgtct tcctttataa
     5041 cgccagtaaa gttaatgtcc gccagactta aaacttcgta aggcgtatta gcaaaaagtg
     5101 acttaaattc tttaagttta tttgcgttat tcgtggcaat gacaatttgt tttttcataa
     5161 ttttataaag tattagtttt taatgctttt tcctttttat ttttttcaac atctttccaa
     5221 ataaaatgga ataaatagta aataagtagc ccagttaaac taaagattac aaagtaaata
     5281 tgcgggaaca ctagtgcaat gattagtgtg gggacaccac cagcaatcat atttgctcta
     5341 atatcaccaa gtatcggcat atttctcctg tcaatccatg gtgaaataaa gaaaccatca
     5401 aaatcccctg gaccagggaa cttcatacca atatagtgga ataaaatatt cgttaattgc
     5461 gctaaaacga tcaaacaaaa taatactgtg accgcgccaa gataatgccg ccatttagtg
     5521 ccaattttat aagcatttat taaatagaaa gcaacaacaa ccattactcc gtgccaaatc
     5581 atcgtatgaa tcgatataga aacttgtaga gtatatacac cagtggcaag aatcatcaca
     5641 gcaagaccac caataagatt gaagattgaa ataaaggagt aaatggcttc tttaatcttc
     5701 tcatttttaa taaatggtgt aagtggatat aaaaagattg gtattgaaca aaattgtaag
     5761 gggaaaccgt accaatgata attagcaagc ccttcatata aatttaagaa aattagttgt
     5821 ttgtataatt caatgccaaa tagtaaaacg ccaaattgaa aaacagtaaa atcaatctta
     5881 tttaactgac caaaaacata gcgtttcgtg ttcgccttgt cggctttaac accacttgaa
     5941 acaacaatga ttgcaataac catggcaaag agccatccaa gatgaaacgg accccacgct
     6001 aagtcatctg gatttttcga tggtaaaaag ttttcaccaa aatgccatct ttcaccaatt
     6061 gcaaacaaaa aaaacattgg ccctaaaatc attaaatatt ttattggtcg ctcgatatac
     6121 caatacttac caggatgata agtttgtaaa tatactttaa gcaatagcaa caaacccatt
     6181 actataacga ataacgctac tatactataa cctactaaat ttgacataca ctctccttaa
     6241 ggcagattga agttattata taacttattg cttttttatt ctattgaaaa aactttattt
     6301 gtttgcgtga agtaataaca atgcgttttt gctggaaaaa atgattaaat aaaaaaagct
     6361 ccctttttgt tgcagtagtt tttgatgcaa ctttaggaag caatttcaat ctagatggag
     6421 c
//
LOCUS       DAOSTA010000049         6146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_11742_length_6146_cov_10.438844, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000049 DAOSTA010000000
VERSION     DAOSTA010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11742_length_6146_cov_10.438844"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..1375
                     /gene="pheS"
                     /locus_tag="PLU08_04455"
     CDS             344..1375
                     /gene="pheS"
                     /locus_tag="PLU08_04455"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020060066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOQ70974.1"
                     /translation="MDKRFESIKEEALSKINNAKSVKALNEVRNEYFAKSGLIATLMS
                     KIRELAPNERKDYGQAVNTLKVFLEEKLGAALREAQSNELSEKLESEKIDLTLPGRNL
                     ETAPQHPFHLVKDEICDIFIGMGYIIAEGDEVEEDLFNFTLLNMPENHPARDMQDTFY
                     INEKLLLRTHTSPVQARVMQKNKNQPLKMISPGKVFRRDDDDNTHSHQFGQVEGLVIG
                     EGISFSHLKATLDLFAKKMFGPKREIRLRSSYFPFTEPSVEVDVSCHVCNGKGCSTCK
                     GTGWIEILGAGMVNPNVLTMNGYDTEKFTGFAFGVGIERVAMLRYGIDDIRRFYQNDI
                     RFLKEFPKR"
     gene            1385..3769
                     /gene="pheT"
                     /locus_tag="PLU08_04460"
     CDS             1385..3769
                     /gene="pheT"
                     /locus_tag="PLU08_04460"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOQ70975.1"
                     /translation="MKISRNALERYIDLSTLSDKEIADGLTFAGIETEDYYKLASGTN
                     LVIGHVLTCIKVEGSDHLSYCTVDVGRLGTREIICGAPNVRSGQKVIVALEGAILPVG
                     EIKRVTIKGHNSEGMICSLSELGVPSELQSHDDLVGIHVLSEDAPVGNTQVLEYLGLD
                     DTIFDIRPLANRSDMLALYNVVRELGAIFDLHYNIPEIEVNPSFKTKLKLEVNTPLVP
                     IFALTEVRNVAIKPSPQWMATFLRKHGHRPVNNIVDIGNYVMLVTGRPLHMYDLDKVK
                     DEHFIVSSDATTKFIALDEKEYNIVPGDQIISDKEKVLCLGGINGAKSSAVTNETKRI
                     AIEVAAFDTAHIRMSVVRHNLPSEASTRFGKGVNLNNTTEAMNLALSLIFDLVPGAEF
                     SNTVTVEKSLPAQPVIYFDKDKINKLLGTTFSEEVMLSTLNRLNITVNEDKSVNLPLY
                     RQDFFSLADLAEEIIRLLGFEHVGNEIPALFTTVGSYDEVQRKRYDIRKLLRSKGLYE
                     VLTYALTDEEKNKTFTYLNKDEPLKLLHPMTPVRSELRLNLLPSVIETLTYNASRQQS
                     DFALFEISNITSENYKGEHLAFAFYGNEVLSRELSKRPYDFFDAKGLIISVLEYLGIN
                     ESRYEFVLNPHKNEEFHPAQSALLKVQGKVVGVFGKLHPLAKEKALLNNEAIIGELNL
                     SALLSLKTSLNKVNPPTRFPFVRRDLALLVNEDLSVKELINVIRKAGGKLISDVSVFD
                     VYTKLKDEPGKKSVALTLTLQDANKTLVEDEIKEVMTKVIEELTKKLQVVVRGI"
     gene            3766..4272
                     /locus_tag="PLU08_04465"
     CDS             3766..4272
                     /locus_tag="PLU08_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70976.1"
                     /translation="MKIKIADGIKLHQTLTAEVDLSDVDLHANYSLKAIKPFTVTVDF
                     DTFTDVVEVHIRGKVTLILECAYTLEHFDYEVEMDDELTFNFVNPNIEAETDDCFYEK
                     GPEIFLDPYLYAIILSYIPLKVIKPGANLPKSGEGYEILSEEEFEAKHQTLDDEFGDL
                     FDKLDLDD"
     gene            4363..4839
                     /locus_tag="PLU08_04470"
     CDS             4363..4839
                     /locus_tag="PLU08_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOQ70977.1"
                     /translation="MYNVVKESGRKWEMKFFGTYYHSLDAKGRLVVPARFRETLKDLK
                     TLYILQGFDGAISVYPEEAYHQELSFLEAQNFKNADARAYIRTIYASIEELEVDAAGR
                     ITLPVRIQKKYDIGQKVIVIGVGDHLEIWDEERYLSYERDACGNLEDLAAKLQEKA"
     gene            4839..5783
                     /gene="rsmH"
                     /locus_tag="PLU08_04475"
     CDS             4839..5783
                     /gene="rsmH"
                     /locus_tag="PLU08_04475"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOQ70978.1"
                     /translation="MSTHYPVLLNEVISGVNVKDDSIIIDLTLGRAGHSSALLFKARN
                     GYLYGFDQDETAIKESREILSKTFQNFTLFHQNFKDFDVALEALGVTGADIILLDLGV
                     SSPQFDVSDRGFSYRYEGPLDMRMDRTNNTLTAAIIVNTYSEQKLKEIFYRYAEHRYS
                     ARIAREIVNARNEQEIRTTTELVEIIKRALPSKELRKKGHPAKTIFQALRIEVNNELG
                     VLEKVLERAPHFLNEGGRLAVISFHSLEDRLVKNVFKKLATLSDEGLHPYTLPDKNRV
                     APFTLVNRKVIVATDEEIELNPRASSAKLRIIEKRRTK"
     gene            5783..6001
                     /locus_tag="PLU08_04480"
     CDS             5783..6001
                     /locus_tag="PLU08_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70979.1"
                     /translation="MRDKLYKTNKKVGYYRFRAFLIGILLVAVGAAALTIPYPYVAKA
                     INEAQARRAQAEEESSEEAPLVTEDLTI"
BASE COUNT         2064 a         1037 c         1267 g         1778 t
ORIGIN      
        1 aaaatttaaa agtttcctaa tctttatttg taaagataag tatgtcatct aacagtttac
       61 attttgtcct attgccacat tcataatttt gccaatttat attatattaa ggtataatat
      121 atgcggcgtt gaaaagaagt agtaatgtct taaagttctt tagatagcga cggtgagatg
      181 gtggaagcac cgctagagaa gagacacgaa ttcaccttgg agccggttga tgacccgttg
      241 actgtcgtta aagtcgtaac taagagtgga aaattaggat ggtaccacgg cgaaagtcgt
      301 tcctatgggg acgctttttt atttaataaa aggagaaatt cttatggata aaaggtttga
      361 aagtataaaa gaagaagctt taagtaaaat aaataacgct aagagtgtga aagcattaaa
      421 tgaagtaaga aatgaatatt ttgctaaaag cggcttaatc gctacgctta tgagtaagat
      481 tcgcgaacta gcgcctaatg agcggaagga ttacggtcaa gctgtaaata cactaaaagt
      541 gtttcttgaa gaaaaacttg gcgcagcact tagggaagca caaagcaatg aacttagtga
      601 aaagttagaa agtgaaaaaa ttgatttaac actacctgga agaaacctag aaacagcgcc
      661 gcaacacccc ttccacctcg ttaaagatga aatttgcgat atctttatcg ggatgggcta
      721 cattattgcg gaaggcgacg aagttgaaga agatctcttt aactttactc ttttaaatat
      781 gccggaaaac catccagcgc gtgatatgca agataccttc tatattaatg aaaagttatt
      841 acttagaaca catacttcac cggtacaagc acgcgtaatg caaaaaaata aaaatcaacc
      901 actaaaaatg attagtccgg ggaaagtatt ccgcagagat gatgatgata atacccattc
      961 ccatcaattt ggacaagttg aaggcttagt aattggggaa ggcatttctt ttagtcactt
     1021 aaaagcaaca cttgacttat ttgcgaaaaa aatgtttggt ccgaaacgag aaatacgctt
     1081 acgttcaagt tatttcccgt ttactgaacc tagtgtggaa gttgatgtta gttgtcacgt
     1141 atgtaacggt aaagggtgct cgacttgtaa aggcactggt tggattgaaa tcttaggagc
     1201 gggaatggtt aatcctaatg tgttaacgat gaatggctac gatacggaga agtttaccgg
     1261 atttgccttt ggtgtcggga tcgaacgcgt agcgatgctc cgctacggga ttgatgacat
     1321 tcgccgcttt tatcaaaatg acatccgttt cttaaaggaa ttcccaaaaa ggtaggtaat
     1381 aattatgaaa atttcacgaa atgcattaga acgatacatt gatttaagta ctttaagtga
     1441 taaagaaatt gcggacggac tcactttcgc gggaattgaa accgaagact attataaact
     1501 cgctagtggt acgaatttag taattgggca tgttttaact tgcattaaag ttgaaggtag
     1561 tgatcacctt tcttactgca cggttgatgt tggtagatta ggaacacgcg aaattatatg
     1621 cggggcgcct aatgtgcgaa gcggacaaaa agttattgtc gcgcttgaag gagcaatttt
     1681 accagtcggt gaaattaaac gcgtcacgat taaaggacat aatagtgaag ggatgatttg
     1741 ttcacttagc gaattaggag tgcctagtga attacagtcg cacgacgatt tagtaggtat
     1801 ccatgttcta agtgaagacg caccagttgg aaatacacaa gttttagaat atttaggatt
     1861 agacgacact atttttgata ttagaccact cgctaacagg agtgatatgc ttgcgctata
     1921 taatgttgtc cgtgagcttg gcgcgatttt tgacttacat tataatattc cggaaattga
     1981 agttaatccg tcttttaaga caaaacttaa actagaagtt aacacaccgc ttgtgccaat
     2041 ctttgcttta acagaagtaa ggaatgtggc aattaaacca tcccctcagt ggatggcaac
     2101 tttcttacgt aaacacggac acagacctgt taataatatt gttgatattg gaaactatgt
     2161 gatgcttgtc acaggtcggc cgttacatat gtatgactta gataaagtaa aagatgaaca
     2221 ctttattgtt agtagtgacg ccactactaa gtttattgcg cttgatgaga aagaatataa
     2281 cattgttcca ggggaccaaa tcattagtga caaagaaaaa gtattatgtt taggcgggat
     2341 taacggcgct aaaagttcag cagtcacaaa tgagacaaaa cgcattgcca ttgaagtagc
     2401 agcttttgac acggcgcata ttcggatgag tgtagtgcgt cacaatttac caagtgaagc
     2461 aagtacgcgc tttggtaaag gtgttaattt aaataacaca actgaagcaa tgaatttagc
     2521 gctctcgctt atctttgact tagtgcctgg tgctgaattt agtaatacag ttacagtcga
     2581 aaagagttta ccagcgcaac ctgtaattta ctttgataaa gataagatta ataagttact
     2641 gggcactact tttagtgaag aagtaatgct aagtacactt aaccgtttaa atattacggt
     2701 gaacgaagat aagagtgtta atctcccact ttaccggcaa gatttctttt ctctagccga
     2761 cttagccgaa gaaattattc gccttttagg ctttgaacat gtcggtaacg aaattcccgc
     2821 attatttacg actgttggga gttatgacga agtacaaaga aagcgttatg acattcgtaa
     2881 attattacgc agtaaagggc tttatgaagt gttaacttat gccttaactg acgaagaaaa
     2941 gaataaaaca tttacttact taaataaaga tgaaccttta aaattactgc acccgatgac
     3001 accagtcaga agtgagttac gacttaatct tttaccaagc gttattgaaa cacttactta
     3061 taacgctagt cggcaacaaa gtgattttgc gctttttgaa attagtaaca taacttcaga
     3121 aaattataaa ggtgaacacc tagcgtttgc attctatggt aatgaagtgt taagtcgtga
     3181 attaagtaaa agaccatatg acttttttga cgctaaaggg ctcattatta gtgtgttaga
     3241 gtatttaggg attaatgaat cgcgttatga atttgtgctt aatccgcata agaatgaaga
     3301 gttccatcct gcgcaaagcg cactcttaaa agttcaaggt aaagtagttg gtgtttttgg
     3361 taaacttcac ccccttgcaa aagaaaaagc attacttaat aatgaagcaa ttattggtga
     3421 acttaattta agtgcattgc ttagtcttaa aacaagctta aataaagtta acccaccgac
     3481 aaggttcccg tttgtaaggc gtgacttagc gctccttgtt aatgaagact taagtgttaa
     3541 agaattaatt aatgttattc gtaaagctgg tggtaagtta ataagtgacg tcagtgtatt
     3601 tgatgtttac acgaaactaa aagatgaacc gggtaaaaaa tcagtagcgc tgacacttac
     3661 tttacaagac gctaataaga cattagttga agatgagatt aaagaagtga tgactaaagt
     3721 tattgaagaa ttaacgaaaa aattacaagt agtggtacgc ggaatatgaa aataaaaata
     3781 gctgatggaa ttaaactcca tcaaactcta acagcagaag ttgatttaag tgatgttgat
     3841 ttacacgcta attattcgct taaagcgatt aaacctttta ccgtaactgt tgactttgat
     3901 acatttaccg atgttgtgga agttcatatt agaggcaaag tcacacttat tttagaatgt
     3961 gcctatactt tggaacattt tgattatgaa gtagaaatgg atgacgaact tacttttaac
     4021 tttgttaatc cgaatattga agcagaaacg gatgactgtt tttatgaaaa aggaccggaa
     4081 atctttttag atccatactt atatgcaatt atcttaagtt atatcccgct taaagttatt
     4141 aagcctggcg ctaatttacc aaagagtggc gaaggttatg aaatcttaag tgaagaagaa
     4201 tttgaagcta aacaccaaac gcttgatgat gaatttggtg atctctttga taagctagat
     4261 ttagacgatt aattattatt ctcatagaat gagcataaaa gaaggggcaa ctcttctttt
     4321 ttatttttta gtgttttttt tgtttacaac gaaaggtcat ttatgtataa tgtagtcaaa
     4381 gaaagtggga gaaagtggga gatgaagttt tttgggactt attatcacag tttagacgct
     4441 aagggacggc ttgtcgtacc tgctcgtttt cgtgaaacac ttaaagactt aaaaacgctt
     4501 tacatcttac aaggctttga tggggcaata agtgtgtatc ccgaagaagc ttaccaccaa
     4561 gaactttctt tccttgaagc acaaaacttc aaaaatgctg acgctagagc gtatattcgt
     4621 acaatttacg caagtattga agagttagaa gtagatgcag caggccgtat taccttacca
     4681 gttcgtattc aaaagaaata tgacattggg caaaaagtaa ttgtaattgg cgtcggtgat
     4741 catcttgaaa tctgggatga agaacgctac ttaagttacg aaagagatgc gtgcggtaac
     4801 ttagaagacc tagctgctaa gttacaggaa aaagcctaat gagtacgcat tatccagtat
     4861 tacttaatga agtaataagt ggcgtaaatg tcaaagatga tagtatcatt attgacttaa
     4921 ccttaggacg agcggggcat agcagtgcct tactcttcaa agcccgcaac ggctatcttt
     4981 acggcttcga ccaagatgaa actgcgataa aagagtcgcg ggaaatactc agtaaaacat
     5041 tccaaaactt cacgctcttc catcaaaact tcaaagactt cgatgttgcg ctagaagcac
     5101 ttggagtcac aggcgctgac atcatcttac ttgacttagg tgtcagtagt ccgcaattcg
     5161 atgtaagtga ccgcggcttt agttaccgct atgaaggtcc acttgatatg cgaatggacc
     5221 ggacgaacaa taccttaaca gcagcgatta ttgtcaacac ttattcagaa caaaaactaa
     5281 aagaaatatt ctaccgctac gcggaacatc gctacagtgc ccgcattgct cgcgaaatcg
     5341 taaatgcgag aaacgagcaa gaaataagaa caacaactga gttagtcgaa attataaaaa
     5401 gagccttacc aagcaaagaa ttacgcaaga aaggccatcc cgctaaaacg atcttccaag
     5461 cacttcgcat tgaagtaaat aacgaattag gagtacttga aaaagtcttg gaacgcgctc
     5521 cacacttctt aaatgaaggg gggagactcg cagtaataag cttccatagt cttgaagacc
     5581 gcttagtaaa aaatgtcttt aaaaagttag cgacactgag tgacgaagga ctgcatcctt
     5641 acacactacc tgataaaaat agagtagcac ccttcacact tgtcaatcgt aaagtcatcg
     5701 tcgccactga cgaagaaata gaactcaacc cacgcgctag tagtgcaaaa ctgcgaatca
     5761 tcgaaaaaag gaggactaaa taatgcgtga caaattatac aaaacgaata aaaaagtcgg
     5821 atactaccgc tttagagcct ttcttatcgg aattttactt gtagcggttg gagcggcagc
     5881 tttaacgatt ccttatccgt atgtcgctaa agcgattaac gaggcacaag cacgccgtgc
     5941 tcaggccgaa gaagaaagca gtgaagaagc tcctttagtt actgaagatt taaccattta
     6001 gtttttttcc tttctttaaa gccttagtga actcttcccc ttttcctctc tcactaaggc
     6061 ttttttctta aatttgtgat aattaagcaa atattaatta gttgcaattg ttattattcg
     6121 ttaattattg taaaataatt acatta
//
LOCUS       DAOSTA010000050         5771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_12683_length_5771_cov_8.957663, whole genome shotgun sequence.
ACCESSION   DAOSTA010000050 DAOSTA010000000
VERSION     DAOSTA010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12683_length_5771_cov_8.957663"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1073
                     /locus_tag="PLU08_04485"
     CDS             69..1073
                     /locus_tag="PLU08_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ70980.1"
                     /translation="MSDAYKDRVTIYEVAAAAGVSLATVSRVINNHPNVTPATRAKVQ
                     NAITRLGYRPSALAQALATSKTTNIGLIIPSANYVFISNMLHGIVMRANSLGYLITLF
                     VTGTEKEDALDAMDKLISSHVDGAIIFDDLFEEEEVTQITNYQVPVVVVNNRLDEDEK
                     VACITFGYENTLREDIIRHHLKTSDVMMHFVALNDNGRLLDRLQRSFVNTHKEENREY
                     KIHKVQDGYHAHYAYFKELFKTTKEGFFVAYRDSLGRAIVNAALENGLRIPEDIEVIS
                     LIGTRYAVQGRPKLSSMILDMEEVGNKALEMLIEILEGKTHLPRQKVVAEYIKLDTTR
                     "
     gene            1085..2335
                     /gene="tyrS"
                     /locus_tag="PLU08_04490"
     CDS             1085..2335
                     /gene="tyrS"
                     /locus_tag="PLU08_04490"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOQ70981.1"
                     /translation="MNIFDELEYRGLIKDYSNAANIKEMLNTPQKIYCGFDPSASSMH
                     MGNFVMIMTLMRLQRAGHKIIAVVGGATGMIGDPSGKSAERNLLDKDALRHNTAQIRA
                     QLEKYLDLSDPQKGEIVNNYDWHGNINVLEFLRDYGKYFTVNYMLAKDTVARRLETGI
                     SYAEFSYMLLQAVDFLYLYEHYACTMQIGGSDQWGNLISGLDLIRKVKGADAKVEVMT
                     AHLLTDAEGNKFGKSVDGALFLDAKKFSPYKLYQYFYNVGDADAEKYLKVFTFLSREE
                     IEAITSEHKEAPHRRLAQTRLAEEIVTIVHSKEALLHAQNMSTALFSGNVKDLSAEDI
                     EDLFAHALVGLDSDQKLEDVLITLKAATSKREAREFIRGNSISVNGERLTDPELIITK
                     DYLLHDKYLIIRRGKKNYFVAEVK"
     gene            complement(2402..3010)
                     /gene="rpsD"
                     /locus_tag="PLU08_04495"
     CDS             complement(2402..3010)
                     /gene="rpsD"
                     /locus_tag="PLU08_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOQ70982.1"
                     /translation="MRDTGSVWKKSRRLGYSILETGEELRKRPYGPGQHGNNRRRKVS
                     EYGKQLMEKQKLRHTYGVNERQFKRLFLLAKKNKEVVTGLAFIQILESRLDNLVFRLG
                     LAATRNQARQLVNHGHIEVDGQKVDIPSFLVPVGSTITVRENSRDLKLFKFNLEKVGR
                     TVPFVTLNAEKLSGTFDRMPERTEVAQDIQENLIIEFYNRSM"
     gene            complement(3241..3405)
                     /locus_tag="PLU08_04500"
     CDS             complement(3241..3405)
                     /locus_tag="PLU08_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOQ70983.1"
                     /translation="MSAADIIILIVVIILLGGIIFYLVRRNKKKCYSCSYAKSCETYC
                     HVIKENDPKE"
     gene            complement(3402..5372)
                     /gene="feoB"
                     /locus_tag="PLU08_04505"
     CDS             complement(3402..5372)
                     /gene="feoB"
                     /locus_tag="PLU08_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOQ70984.1"
                     /translation="MKIALIGNQNSGKTSLFNLLTGNNQKVGNWPGVTLERKTGILRT
                     SNAEVIDLPGIYSLSPYSHEENIVRRFLLEEKVDLIINIIDSTAIERSLYLTTQLFNF
                     NIPMVIALNMTDLAFEQGIRIDVNKLSKALSVPVVPISCLKRTGIKDLIMLITKDTPT
                     STNYLTIYSNPLQETINKTAELLPIDAKEFLAIKLLENDIDLAPRIDLDVSKDIKKLN
                     DHYKLGISEVLANERYNFIEKVRDIAVSSREKPKLLTDKIDRILLNKYLAIPIFIIIM
                     LLVYFLAAGPVGIFLSSLVEQGFAALNEGLATFLTNVGASAWSVSLVVDGILTGVTSI
                     LSFVPQLLILFFMIDILEASGYMSRIAFFFDRVFRKVGLSGKALIPFIIGTGCSVPAI
                     MASRVIEDERERKLTVLLTPFIPCGAKLPIITLFAAYFFPDSMGLVSASLYFLSIIII
                     LLTALLLKRILYKQKYSPFIQELPTYKAPNMRYVLRDTGHKTWSFITNAGSVILFASV
                     ILWLLLNFNWKMQFGVAIEKSILSDIGRLFSFVFYPFLGTNSWAAAVSIIQGLIAKEQ
                     VVSSLSIIAKLSGGNTIFESPLFNFFTPASAYAFMAFNLFSAPCFGAIGAMNHEFGNR
                     RTMIKALLLQTIAALVIATLIFQVGTFVEGLL"
     gene            complement(5369..5611)
                     /locus_tag="PLU08_04510"
     CDS             complement(5369..5611)
                     /locus_tag="PLU08_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOQ70985.1"
                     /translation="MSDTLTLDKLTIGQKARVLRINDDNPALKQRLLDMGITTGVIVK
                     IDKISPLGDPFCLELRNYRLLVRRADVSAIEVEVIE"
BASE COUNT         1891 a         1029 c         1091 g         1760 t
ORIGIN      
        1 gctttctctt ctacattgac aatgaaaata ttttcaagta aaattggtat gaaagaaggc
       61 tttttaatat gagtgatgca tataaagaca gagtaacaat ctacgaagta gcagcagcgg
      121 ctggcgttag cctagcaact gtgtcgcgtg tcattaataa tcatccaaat gtcacaccag
      181 cgacgcgggc taaagtccaa aacgccatta cgcgccttgg ttatcgtcca agtgctttag
      241 cgcaagcgct agccacgagt aaaacgacga atattggctt aattattcct agtgccaact
      301 atgtatttat ttcaaatatg ctccacggca tcgtgatgcg tgcgaatagt ttagggtatc
      361 taattacttt atttgtcact ggtacagaaa aagaagatgc acttgacgct atggataaat
      421 taattagttc gcatgttgat ggggcaatta tctttgatga cttatttgaa gaggaagaag
      481 taacgcaaat tacgaattat caagttcctg ttgttgtcgt taataatcgt cttgatgaag
      541 atgaaaaagt tgcatgtatt acttttggtt atgaaaatac attacgtgaa gatattattc
      601 gtcatcacct aaaaacaagt gatgtgatga tgcattttgt agcacttaat gataatggtc
      661 gtttgttaga tcgtttacaa agaagctttg ttaacacaca taaagaagaa aatcgtgaat
      721 ataagattca caaagtacaa gacggatatc acgcgcatta tgcttacttt aaagaactat
      781 ttaagacgac aaaagaaggg ttctttgttg cttatcgtga ctcattagga cgcgcgattg
      841 ttaatgccgc gcttgaaaat ggcttacgca ttccagaaga cattgaagta attagtttaa
      901 ttgggacacg ctatgctgtg caaggccgcc caaaattatc aagtatgatt cttgatatgg
      961 aagaagttgg taataaagca ttagaaatgc taattgaaat attagagggt aagacacact
     1021 taccgcgaca aaaagttgta gccgaataca taaaacttga tacaactcgc tagaaaggtt
     1081 cgttatgaat atatttgatg aattagagta tcgtggttta attaaagatt actctaacgc
     1141 tgctaacatt aaagaaatgt taaatacgcc acaaaaaatc tattgtgggt ttgacccaag
     1201 tgcttcaagt atgcatatgg gaaattttgt gatgattatg actctgatgc gcttacaaag
     1261 agcagggcat aaaattattg ctgttgtcgg tggtgcgacg gggatgattg gtgacccaag
     1321 cggtaaaagc gctgaacgta atttacttga taaagatgct ttacgacata ataccgcgca
     1381 gattcgcgct caattagaga aatatctaga tttaagtgac ccccaaaaag gtgaaattgt
     1441 taataattat gactggcatg gtaacattaa tgttttagaa tttttacgcg attacggcaa
     1501 atattttacc gttaactata tgttagcaaa agacactgtc gcgcgtcgtc ttgaaactgg
     1561 aattagctat gctgaattta gttatatgct cttacaagcc gttgactttt tatatctcta
     1621 tgaacattac gcttgcacta tgcaaattgg tggtagtgac cagtggggca atttaatcag
     1681 cggacttgac cttattcgta aggtaaaagg tgctgatgca aaagttgaag taatgacggc
     1741 acacttatta acagatgcgg aaggtaataa atttggtaag agtgtggacg gcgcactttt
     1801 ccttgacgca aagaaattta gtccatataa gctttatcaa tacttttata atgtcggtga
     1861 cgctgatgct gaaaagtatc ttaaagtatt taccttttta agtagggaag aaattgaagc
     1921 aattactagt gaacataaag aagcaccaca ccgccggctt gcccaaacta gactcgccga
     1981 agaaattgtg acaattgttc atagtaaaga agccttactc catgcccaaa atatgagcac
     2041 tgcccttttt agcgggaatg ttaaagattt aagtgccgaa gacattgaag atttattcgc
     2101 ccatgcacta gtggggcttg acagtgacca aaaactcgaa gatgttctta ttacgctaaa
     2161 agcggcgaca agtaaacgtg aagctcgtga atttattcgt ggtaacagca tcagtgtaaa
     2221 tggcgaacgt cttactgatc cagaattaat tattactaaa gattatttat tacacgataa
     2281 atatttaatt atccgccgtg gtaagaagaa ctactttgta gccgaagtaa agtaaaaagc
     2341 tgcaaattga aaatcaaaaa aactccgcta ttatgcggag tttttgatta agtctgctta
     2401 attacattga acgattgtag aattcaatga ttaagttttc ttggatgtct tgagcaactt
     2461 cagtgcgttc tggcatccgg tcaaatgtac cgcttaactt ttcagcgtta agcgtcacaa
     2521 atggaactgt acgaccaact ttttcaagat tgaacttaaa gagttttaag tcgcgactat
     2581 tttcacggac cgtaatggtg ctgccaactg ggactaagaa gcttgggata tcaacttttt
     2641 gaccgtcgac ttcaatatgg ccgtggttaa cgagttgacg agcttggttg cgggtcgctg
     2701 cgagtccaag acggaacact aagttatcaa ggcgtgattc aagaatttga ataaacgcta
     2761 aaccggtaac gacttcttta ttctttttcg ctaataagaa aagtctttta aattggcgtt
     2821 catttacacc ataagtatgg cgtaattttt gtttttccat taattgttta ccgtattcgc
     2881 tgactttacg acggcggtta ttaccgtgtt gaccaggacc atacggacgt tttcttaact
     2941 cttcaccggt ttctaaaata gaataaccaa gacgacgaga ctttttccaa actgaaccag
     3001 tatctctcat gttttttcct ccttttcttt acttgcaggc aaacaaaagg tgcaaacccc
     3061 tcgtttctgg atgatgattt tattcgtttc gccttatagc tttggttact acgatgagta
     3121 ttccttaatc atcattagac aaacggtgtt tacatgctgc ttacatgctt tgttataata
     3181 acttaaatta taatttgtgt caatctaaat tgtgttaaac tccttataaa ggagtgtttt
     3241 ttattctttt gggtcatttt ctttaattac atgacaataa gtctcgcaac ttttagcgta
     3301 agagcacgag taacatttct ttttattacg gcgcacaaga tagaaaataa tcccacctaa
     3361 aaggatgatc acaacgatta aaatgataat atccgcagca ctcataataa accctccacg
     3421 aaagtcccaa cttgaaagat taaagtggca atgactaacg ctgctattgt ttgcagaagc
     3481 agcgctttaa tcatagtccg acgattacca aattcatgat tcatagcccc aatcgctccg
     3541 aaacacggcg cactaaataa attaaaggcc ataaaagcat aagctgaagc aggagtaaag
     3601 aaattaaaaa gcggactttc aaagatggtg ttgcccccac taagtttagc aataattgaa
     3661 agtgaagaaa caacttgttc tttggcaatt agcccttgaa taatactaac cgcagccgcc
     3721 caactatttg tgcctaagaa cgggtaaaac acaaacgaga ataagcgtcc aatatcactt
     3781 aaaatactct tctcaatcgc aacgccaaat tgcatcttcc agttaaagtt aagtaaaagc
     3841 cataagataa cagaggcgaa taaaataact gagccggcat tcgtgataaa tgaccatgtt
     3901 ttatgtcctg tatcacgtaa tacatagcgc atatttggtg ctttataagt tggtagttct
     3961 tgaataaatg gtgaatactt ttgcttataa aggatgcgtt ttaaaagcaa agctgttagt
     4021 aaaataatga taattgatag gaaatataat gaggcactta ctagtcccat tgaatcaggg
     4081 aagaagtagg ccgcaaagag cgtaataatc ggtaattttg cgccgcacgg aataaaaggc
     4141 gtaagtaaaa cagttaattt tcgttcgcgt tcatcttcaa taacgcggct tgccataatc
     4201 gccggtacac tgcatcccgt accaataata aaagggataa gtgctttacc cgataaacca
     4261 actttacgga aaacacgatc aaaaaagaaa gcaatgcggg acatataacc acttgcttct
     4321 aagatatcaa tcataaagaa taaaatgagt aattgcggca caaaactaag aatcgaagtc
     4381 acgcctgtta agatgccatc aacgactaaa ctaacagacc acgcagaagc cccaacatta
     4441 gtgagaaaag ttgctaaacc ttcgtttaac gcggcaaaac cctgctcaac tagtgatgag
     4501 agaaaaatgc caactggtcc tgctgctaag aaatagacaa gtaacatgat aataataaat
     4561 atcggaattg ctagatattt atttagtaaa atccgatcaa ttttatccgt taaaagtttt
     4621 ggtttttcgc ggcttgaaac agcaatatca cgaacttttt caataaaatt atagcgctca
     4681 ttcgctaata cttcgcttat cccaagtttg taatgatcat ttaatttttt aatgtctttg
     4741 cttacatcaa gatcgatgcg tggcgctaaa tcaatatcat tttctaatag ttttattgct
     4801 aaaaattctt tagcgtcaat cggtaaaagt tcggctgttt tattgatagt ttcttgcagt
     4861 ggattgctat atatagttag gtaattagta gaagtagggg tatctttagt aattaacatt
     4921 attaaatctt ttatccctgt tctttttaaa catgatattg ggactacggg aacacttagt
     4981 gccttgctaa gtttatttac atcaatccga atcccttgtt caaacgctaa gtcagtcatg
     5041 tttagtgcaa tgaccattgg aatattaaaa ttaaataatt gcgtagtaag atataagcta
     5101 cgttcaatcg cggtactatc aataatgtta attattaagt caactttttc ttctaataag
     5161 aagcgccgga caatattttc ttcatgacta tatggagaga gactatagat acctggtaaa
     5221 tcaatcactt cggcatttga ggtgcgtaaa ataccagttt tgcgctcaag cgttactcct
     5281 ggccaattac caaccttctg attattacca gttaacaaat taaatagtga agttttacca
     5341 ctattttgat taccaattag tgcaattttc attcgataac ctcgacttca atcgcactca
     5401 catcagcgcg acgcactaat aagcgatagt tgcgtaactc taggcaaaaa gggtcaccaa
     5461 gtggtgatat tttatcaatt ttaacaatga caccagtcgt aattcccata tctaaaagtc
     5521 gttgttttaa tgctggatta tcatcattaa ttcttaaaac acgggctttt tgcccgatgg
     5581 taagtttatc tagagtaaga gtatcgctca cttaggaata atcccttcta gtggttatac
     5641 aaagtataat cacacaccaa aatataccct aatagccgct tattatcaag tatttaacat
     5701 aataaatata tgttaaccgt taaaggaaaa ggttaaattt acatccttct tgtcttcatt
     5761 ataatgttta t
//
LOCUS       DAOSTA010000051         5634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_13082_length_5634_cov_9.718229, whole genome shotgun sequence.
ACCESSION   DAOSTA010000051 DAOSTA010000000
VERSION     DAOSTA010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13082_length_5634_cov_9.718229"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1129
                     /gene="trpS"
                     /locus_tag="PLU08_04515"
     CDS             149..1129
                     /gene="trpS"
                     /locus_tag="PLU08_04515"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOQ70986.1"
                     /translation="MMRVLSGIKPTGKLTLGNYIGALKNFSQYQSDHEVFIFIADLHA
                     LTLPIDPDELRNNTKLLAAFYLAAGLDPNKVTIFKQSDVSAHAELNAILQNYLYMGEL
                     SRMTQFKEKSQSMDHSGIGLGLFAYPVLMASDILLYEANIVPVGEDQIQHIEITRDLV
                     VRFNNRYGKVLVMPEPQVTRVGKRIMSLSDPSVKMSKSESGKGDIYLTDDLKTMRKKI
                     MSAVTDSGSEIYVDEENKPGITNLLTIYAALTDKSLESCAAQFKDYRYGDFKKEVANV
                     VIDTLGPFQAKAMSFLESGEVDRVLSEGAKKATVIANKTLVKVKKAVGLL"
     gene            1126..1938
                     /locus_tag="PLU08_04520"
     CDS             1126..1938
                     /locus_tag="PLU08_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOQ70987.1"
                     /translation="MNKDKYLIAIDLDGTLLTDDKKITPKTRNYLNALHDEGHLIVIA
                     TGRPFRAAYGFQQQLGVNGPLVTYNGSLTSNPHDASFPTRTRKFDRFALKDMVDAIGF
                     DALDNLMIETEHVIYLLHEDEALNNFFWNDRERIIYGNPFLHLNEDPLALIFLFKNTD
                     PEYKKEVSRIVESFPDLHIRFWNLEGYAELWIGESTKKDGLEHIANFYNFPQKRIIAF
                     GDASNDKEMLAWANYGVWMKNGVPSVEAYANMKTTEDNNNDGIVSTLQKIIK"
     gene            complement(1928..3622)
                     /locus_tag="PLU08_04525"
     CDS             complement(1928..3622)
                     /locus_tag="PLU08_04525"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOQ70988.1"
                     /translation="MQNIDALYEKWLKHPEMPKKLVSELKKMSAKAKTDAFYQNAEFG
                     TAGMRGILGAGTNRINIFTIRKAAYGFGLYVIKHVRNAKERGIVIAHDNRHMSEEFTE
                     ESMKTLRSLGIKTFVFDELKPTPLLSYAVRHLNAAGGIMITASHNPKNYNGFKVYDDT
                     GAQLTPEKVDLLIAELKTLPDYLDIKIDDKVNGYILLSDDVELTYYSKVMKIQLRRDL
                     PKKDFKIVFSPQHGTGRKLGMRALNVIGYNVIPVTEQMEPDPNFSGTKSPNPEEKAAY
                     SEAIKYARAYGAHLIITTDPDADRLGIVAKNKKGDFDYLTGNQSAALLLDYLCKTNIE
                     LGKDIKGKYLYTTIVSSSLANEVAESYGLKVKEFLTGFKFIGAQIAEDDSKNEDYFFF
                     GFEESYGLLAAPFVRDKDAIQAIVLYSEMALYYYLQGKTLGEVLDELYKKYGYHFDVT
                     KSVAFPGAAGQSEMQEILKRLRDNPPVTIGGLNILRYDDYLTSKSVTSGETTTLTLPK
                     SNVIRYHLEGGTTISIRPSGTEPKCKIYYGIITENEKEAQDLLTLLEADIKVRFTL"
     gene            complement(3626..4549)
                     /locus_tag="PLU08_04530"
     CDS             complement(3626..4549)
                     /locus_tag="PLU08_04530"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOQ70989.1"
                     /translation="MEKLTFIISDIEANQRLDKYLKKIMPNAPQSFIYKLIRKKDIRV
                     NNKKSDAAYILNEDDTVVIFLTEAQVEEFIVPYTFTKIRRPLNIIYEDENILVVNKDT
                     GLLVHGTEFENELTLTNIVLTYLYDRGEFDPLRRGYIPSPVSRIDRDTSGVVIFSKKQ
                     SVHQVLANAFTQEDEVIRLYRLVVYGTLNKDEGAIELPLKRENGQTIPASDGQKAITH
                     YVVKKKGRTKTYVEAQLLTGRQHQIRAHFKAIGHPLVGDTKYGSSTEQGPLSLNAYKV
                     EFTALPAPLDYLNGHEFIADNTAYLLEMLGD"
     gene            4612..5406
                     /locus_tag="PLU08_04535"
     CDS             4612..5406
                     /locus_tag="PLU08_04535"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HOQ70990.1"
                     /translation="MFKKEGFAEPAGLFSLSHLGLVLFFGTLAVLFYALTKKWTKAQV
                     MRFFKIYSLVLIALEIFKIIWNIVNYGTSAESLNRFIPLYYCSIFLYAFVIVAFTKGK
                     IQKAAYTWIMYGGLASGLAFLIYPSSSLLFYPFYHFLCLHSILFHMNFILIGFLIFRH
                     ELYTPNVKDFWYFVSFSLVFMSLAVILNKVLGTNLMFFEKPLAILPFETLNEWSPLFF
                     QSLIFILQLFGPFIGTHFVFYLFSRFTPFKIYKRNEENLTTNNDNI"
     gene            5435..5525
                     /locus_tag="PLU08_04540"
     tRNA            5435..5525
                     /locus_tag="PLU08_04540"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5469..5471,aa:Ser,seq:gga)
BASE COUNT         1755 a         1055 c          956 g         1868 t
ORIGIN      
        1 cacataaaat cattaaaaca cttaatcttg ttaattttaa ttattcatct atccacctga
       61 aatgaatttt aattaatttt tgacaatgta tgcgcttagt tgactataaa aataatatag
      121 attataattt ttaagattaa aaggagtaat tatgcgagta ttaagtggaa ttaaaccaac
      181 cggaaaacta acacttggta actacattgg ggcgttaaaa aacttctccc aatatcaaag
      241 tgaccatgaa gttttcatat tcattgccga cttacacgcc ttaacgctgc cgatagaccc
      301 tgatgagtta cgcaataata caaagttatt agcagccttc tatctagcag ccgggcttga
      361 tcccaataaa gtaacaatat ttaaacaaag tgatgttagt gctcacgctg aattaaatgc
      421 gatcctacaa aactatttat atatgggtga acttagtcgt atgactcaat ttaaagaaaa
      481 aagtcaatca atggatcata gcggtattgg cttaggttta tttgcctacc ccgtcttaat
      541 ggctagcgat attttacttt atgaggctaa tatcgtgccg gtcggcgaag accaaattca
      601 acatattgaa attacacgcg acctagttgt gcgctttaat aaccggtacg gaaaagtttt
      661 agtaatgccc gagccacaag taacgcgtgt cggtaaacgc attatgtcac taagtgaccc
      721 gagtgttaaa atgagcaaaa gcgaaagcgg taaaggtgat atatatctaa ctgatgactt
      781 aaaaacaatg cgcaagaaaa ttatgagcgc tgttactgat tccggtagtg aaatttatgt
      841 tgatgaagaa aataaacctg ggattacaaa tttacttaca atttacgctg cgctaactga
      901 taaaagcctt gaatcatgcg ctgcgcaatt taaagattac cgttatggcg actttaaaaa
      961 agaagtcgct aatgttgtta ttgatacact tggtcctttc caagcaaaag caatgtcttt
     1021 tcttgaaagc ggggaagttg atcgcgtcct aagtgaagga gcaaaaaaag caacagttat
     1081 cgctaataaa acattagtga aagtaaagaa agcagtagga cttttatgaa taaagacaaa
     1141 tatttaattg ccattgatct cgatggcaca ctcttaactg atgataaaaa aatcacccct
     1201 aaaactcgta actatctaaa tgcccttcac gatgaaggac atttaattgt catcgccact
     1261 ggacgaccat ttcgcgcagc atatggtttt caacaacaat taggagtaaa cgggccactt
     1321 gttacatata acggctcttt aacatctaat ccacatgatg cttcattccc aacacgcaca
     1381 cggaaatttg atcgctttgc gcttaaagat atggtagacg ctatcgggtt tgatgcctta
     1441 gataatttaa tgattgaaac tgaacatgtt atttatttat tacatgaaga tgaagccctt
     1501 aataatttct tctggaatga tcgtgaacgc ataatttatg gtaacccctt cttacattta
     1561 aacgaagacc ctttagcgct tatattctta tttaaaaata ctgatcctga atataaaaag
     1621 gaagtatcgc gtattgtcga atcattccct gatttacata ttcgtttctg gaatttagaa
     1681 ggttatgcag aattatggat tggcgaaagc acaaaaaaag acgggcttga acacattgct
     1741 aacttttata atttcccaca aaagcgcatc attgcttttg gcgacgcatc taatgataaa
     1801 gaaatgctag cgtgggcaaa ttatggtgtg tggatgaaaa acggtgtacc aagtgttgaa
     1861 gcttacgcaa atatgaaaac aactgaagat aataataatg atggtatcgt aagcacctta
     1921 caaaaaatta taaagtaaag cgaactttaa tatcagcttc aagaagcgta agtaaatctt
     1981 gcgcttcttt ttcattttca gtaataatac cgtaataaat tttgcacttt ggttcagtac
     2041 cacttgggcg aatcgaaatc gttgtgcccc cttcaaggtg ataacgaatg acattactct
     2101 ttggtaatgt taaagttgtt gtttcgccac ttgtcacact tttactagtt aaataatcat
     2161 cgtagcgtaa aatatttaac ccaccaatcg tgacgggcgg gttatcacgt aatcgtttta
     2221 agatttcttg catttcactt tgaccggcag cacctgggaa agctactgac ttagtaacat
     2281 caaagtgata accatatttt ttgtaaagtt catctaaaac ttcaccaagt gtcttgcctt
     2341 gtaagtaata atataaagcc atttcactat aaaggacaat tgcttgtatc gcatctttat
     2401 cacggacaaa tggagcggcg agtaacccgt aactttcttc aaaaccaaag aagaaataat
     2461 cttcgttttt actgtcatcc tcggcaattt gtgccccaat gaacttaaag ccagttaaaa
     2521 attctttaac ttttaaacca taactttccg ctacttcgtt agcaagacta ctacttacaa
     2581 ttgtcgtgta taagtatttt cctttaatat ctttgcctag ttcaatattc gttttacata
     2641 agtaatctaa aagtagcgct gcactttgat tacctgttaa ataatcaaaa tcgcctttct
     2701 tatttttagc tacaatccct aaacgatcag cgtcaggatc agtcgtaata attaaatgcg
     2761 caccatatgc tctagcgtat ttaattgctt cgctgtaggc tgctttttct tcgggattag
     2821 gactttttgt gccggaaaaa ttcgggtctg gttccatttg ttcggtaaca ggaataacat
     2881 tatagccaat aacattaagc gcccgcattc caagttttct ccccgtgcca tgctgcggtg
     2941 aaaagacaat tttaaagtcc ttcttcggta agtcgcggcg taattgtatt ttcattactt
     3001 ttgagtaata agttaattca acatcatcac ttaaaagtat atatccatta actttgtcat
     3061 cgatttttat atctaaatag tcaggtagcg tttttaattc ggcaattaat aaatcaactt
     3121 tttccggtgt aagttgtgca cctgtatcgt cataaacttt aaagccgtta tagttttttg
     3181 ggttatgcga agcggtaatc attattccgc ctgctgcatt taaatgccgc actgcataac
     3241 ttagtaatgg tgtaggtttt aattcatcaa acacgaaggt tttaattcct agactgcgca
     3301 atgttttcat tgattcttcc gtaaattctt cactcatatg acggttatca tgggcaataa
     3361 caatcccgcg ttctttagcg tttctcacat gtttaatcac atataaacca aagccatatg
     3421 ccgctttacg aatcgtaaaa atattaattc tatttgtacc cgcgcctaaa attccgcgca
     3481 ttcccgctgt gccaaactcg gcattttgat aaaaggcgtc tgttttagct ttcgcgctca
     3541 tcttttttaa ctcgctaaca agttttttag gcatttcagg gtgttttaac catttttcat
     3601 acagtgcatc aatgttttgc ataatttaat ctcctaacat ttctaataaa taagccgtgt
     3661 tatcggcaat aaattcatga ccatttaaat aatcaagtgg ggctggtagt gcagtgaact
     3721 ctaccttata agcgtttaaa gaaagtggtc cttgttcggt actactaccg tatttagtgt
     3781 caccaacaag cggatgacca atcgccttaa aatgcgctct tatttggtgt tggcgccccg
     3841 ttaaaagctg cgcttccaca tatgttttag tgcgtccctt tttcttaact acataatgag
     3901 ttatcgcttt ttgaccatca ctagcaggaa tcgtttgacc attctccctt tttaaaggga
     3961 gttcaatcgc tccttcatcc ttgtttagtg taccataaac gactaaacga tataaacgaa
     4021 tcacttcatc ttcttgcgta aatgcatttg ccagcacttg atgaacgctt tgcttcttac
     4081 taaaaataac aacaccactt gtatcacgat caatacggct gactggcgaa gggatataac
     4141 cgcgccgcag cgggtcaaat tcaccacgat cataaaggta agttaacaca atattagtta
     4201 aggttaattc attttcaaat tcagtaccgt gcactaaaag tcctgtatct ttattaacca
     4261 ctaaaatatt ttcatcttca taaattatgt taagtggtcg tcttattttc gtaaaagtat
     4321 aaggcacaat aaattcttca acttgcgctt ctgttagaaa aatgacgacg gtgtcgtctt
     4381 catttaatat gtaagcagca tcactcttct tattatttac tctaatatct tttttacgaa
     4441 ttaatttata aataaaactc tgcggggcat taggcataat cttttttaaa tacttatcaa
     4501 gacgttgatt agcctcaatg tcactaatga tgaatgttag tttctccata ctttaatttt
     4561 accaaatatc agtgcgcata tggtaaaatt agacaatgaa aacgagggca aatgtttaaa
     4621 aaagaaggct ttgctgaacc tgcgggactc tttagtctta gtcacctcgg attggtgctc
     4681 ttttttggga cgcttgcagt gcttttttat gcattaacga aaaaatggac aaaagcgcaa
     4741 gtgatgcgtt tctttaagat atatagtctt gtattaatag cgttagaaat atttaaaata
     4801 atttggaata tcgtaaatta tggcactagt gctgagagtt taaatcggtt tatcccgctt
     4861 tattattgct ccatttttct ctatgctttt gtaattgttg catttaccaa aggtaaaatt
     4921 caaaaggcgg cttatacttg gataatgtat ggcggacttg cttcaggttt agccttttta
     4981 atatatcctt ctagttcgct tttgttttat cctttctacc acttcttatg tttgcattct
     5041 atcttattcc atatgaattt tatcttaatt ggttttttaa tcttccgtca tgaactctac
     5101 actccaaatg ttaaagactt ttggtacttt gtgagctttt cactcgtatt tatgtcacta
     5161 gcggtaatct taaataaagt gttaggcact aatttaatgt tctttgaaaa acctttagcg
     5221 atccttccgt ttgaaacatt aaacgaatgg tcaccactct ttttccaatc cttaatcttt
     5281 attctgcaat tatttggacc ttttattggc acacactttg tcttttatct ctttagtcgt
     5341 ttcactccat ttaaaatata taaaaggaat gaagaaaact taactacaaa taatgacaac
     5401 atataattaa atttgctata ataaccgcac tgctggagaa atacccaaga ggctgaaggg
     5461 gctggcttgg aaagctagta ggtctgtcac aggacgcgag ggttcaaatc cctctttctc
     5521 cgccagataa gtaaattagt ccgcatcata gtcggacttt tttataaatg taataaagcg
     5581 ggttagataa catacttatc tttacaaata aagattaaat ttttaaaaac aaac
//
LOCUS       DAOSTA010000052         5289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_14225_length_5289_cov_12.241880, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000052 DAOSTA010000000
VERSION     DAOSTA010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14225_length_5289_cov_12.241880"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..519
                     /locus_tag="PLU08_04545"
     CDS             166..519
                     /locus_tag="PLU08_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOQ70991.1"
                     /translation="MLKDTNINVRVNSDIKNEAETILNKLGLNLSSAIDLYLAQIIKT
                     RGIPFEVKLPNEENKEKVLQIGQIINSLGGAPTTPELNKIIELYAQNDISYDVAIYAI
                     KEILNKLYCETLLLY"
     gene            complement(516..1118)
                     /locus_tag="PLU08_04550"
     CDS             complement(516..1118)
                     /locus_tag="PLU08_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ70992.1"
                     /translation="MKKKKLFWLILPFTLIMFALLFLRYPIMPTNGTYKVFHSPKKHV
                     ENVTTLIHQKKDTIDVEWEDVEVTPLYNYKDNFNYLLVDLYPQGFTILKVIRTSPFSV
                     KASQYAMPSVYGRHYYYSDEDTSPQYTSIYAERDVKNEKLYLLQVKNSHGHGHIPAIK
                     GDNCYINLLTLEEIPLNYYEIDEPYAMYEWWFAYMVAFRF"
     gene            complement(1206..1892)
                     /locus_tag="PLU08_04555"
     CDS             complement(1206..1892)
                     /locus_tag="PLU08_04555"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOQ70993.1"
                     /translation="MCRRINTNKIAYVSLVMLLALGLKASIPKKEFMANDHFIFEINH
                     RDKYATAIELTEAGKEQEVLVVPPSIRGFPVRYIGKPKRFFQPLDINALKLTDKQIKI
                     YLPYSLRGQMWLASVRTVEAILALANLDDFDKVLTINSFPEIKLHYLNGKNKLNTFYM
                     YNFESSLNEGYYFMDYINGSNPYVIPSDPVRKGYTFAGWYYQKECATLWNNEVPKSES
                     ESLTLFAKWI"
     gene            complement(2140..2883)
                     /gene="tpiA"
                     /locus_tag="PLU08_04560"
     CDS             complement(2140..2883)
                     /gene="tpiA"
                     /locus_tag="PLU08_04560"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOQ70994.1"
                     /translation="MRKKLLVGNWKMNKTMAETAVFAHASFELLKNAKAKNIDIGVTP
                     PYLSLQTLLNINPKLIIGAQNVHHLEKGAYTGEISIPMLKELGVSWSLVGHSERRTYF
                     NETNESCNLKVLALLENNMTPLYCVGETLQEFEKGVTKEVIKAQIEVGLKGVKDVKEV
                     VIAYEPVWSIGTGKNASKEIAEDICAYIRELLRAKYGGDADLVRILYGGSVKPNNIKE
                     YMAMPNIDGALVGGASLSVESYAELINNM"
     gene            complement(2885..4102)
                     /locus_tag="PLU08_04565"
     CDS             complement(2885..4102)
                     /locus_tag="PLU08_04565"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOQ70995.1"
                     /translation="MAKIIRDLKLANKTVLIRCDFNVPFKGEVISDDNRIRAALPTIK
                     FALDSGAKVVLFSHLGKIKHKGTEEEVEAAKKKNNMVIVFRRLAEILGEDKVFFSPET
                     SGEEVNKRVALLKPGQVLLLQNTRYEKGEEKNDPVLSEKWASFADVFVMDAFGSAHRA
                     HASTVGVPELLKARGKEVALGFLVEKEVEALGRVVHVAKKDRPYIAVIGGFKVSDKIK
                     VIETLLQKCDKVLIGGAMAYTFQVAQGHNIGISPFEPEQVDFAKKCLDTACEKILLPV
                     DAVATDAFQDWTKKITTTDSNIPDGFEGMDIGPKTQKLFADELSKAKMIFWNGPMGVF
                     EQKDFEAGTRAVCEAVSKLKGAFTVIGGGDSAAAVAQFGYKDYFSHVSTGGGASLEMI
                     ENDGHLPGIDVIG"
     gene            complement(4164..5195)
                     /gene="gap"
                     /locus_tag="PLU08_04570"
     CDS             complement(4164..5195)
                     /gene="gap"
                     /locus_tag="PLU08_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOQ70996.1"
                     /translation="MSIKIAINGFGRIGRLAFRRIAETKGMEVVAINDLTDAENLAYL
                     LKYDSAHGRFHVDDISVTADGTAIVFKGKEIPVYAQPDPTQLNWTKHGVDIVLECTGR
                     FRGKEDASVHLQEGVKAVIISAPADKETPMFVYGVNTDKLTKDMTVISGASCTTNALA
                     PVVKVLDDNFGVERGLMTTVHAYTNDQTILDLPKKKAARRGRAGAMNIVPTSTGAAKA
                     IGQVLPHLNKKLDGGALRVPVIDGSMVDLTVLLKKKVTAEEINAVMKKAAEGELKAIL
                     GYTEDEVVSGDIIGDTHGGLFDATLTKVMEVDGEQLVKVMFWYDNEYGYTCQYIRLAQ
                     EVAKVHGVK"
BASE COUNT         1619 a         1054 c          853 g         1763 t
ORIGIN      
        1 aacttaaagt gtgattcaaa taatatgata tataattttc actttttgta atttaaacca
       61 ttaaagtcgc attttatggg gtttttatat taattttaac ccttaaaatc agactctgta
      121 ttgacaatgt taatacatat tagtaaaata aatttgatag aaacgatgtt aaaagacaca
      181 aatataaatg taagagtgaa tagtgatatt aaaaacgaag ccgaaacaat tttaaataaa
      241 ctagggctta atctttctag cgctattgat ttatatttag cgcaaattat taaaacgcgc
      301 ggcatcccct ttgaagttaa attgcccaac gaagaaaata aggaaaaagt tttacaaatt
      361 ggacaaatta ttaattcatt aggtggcgct cctacaaccc cagaacttaa taagattata
      421 gaactctatg cgcaaaatga tataagctac gatgtagcga tatatgcgat taaagaaata
      481 ttaaataaat tatattgtga aacactatta ttgtattaaa agcgaaaagc aaccatatat
      541 gcaaaccacc attcatacat agcataaggt tcatctattt cataataatt aagtggtatt
      601 tcttctaaag ttaatagatt gatataacaa ttatcacctt taattgcagg aatatgacca
      661 tgtccatgcg aatttttaac ttgtaacaaa tataattttt catttttaac atcacgttca
      721 gcataaatac tagtgtattg tggtgaagta tcttcatcac tatagtaata atgacgacca
      781 taaacactag gcattgcata ttgactagct ttaacactaa acggactagt gcggattact
      841 tttaaaatag taaatccttg ggggtacaga tccactaata aataattgaa gttatctttg
      901 tagttgtaaa gtggtgttac ttcaacatct tcccactcaa catcgattgt gtccttcttt
      961 tgatggataa gcgttgttac attttcaaca tgtttctttg gtgaatgaaa aactttatat
     1021 gtcccgtttg tcggcataat aggatagcgc agaaacaaaa gagcaaacat aattaaagta
     1081 aacggtaata ttaaccaaaa caatttcttt tttttcatta ttgtttcctc cctgttgtaa
     1141 ttgtatattt tttttcttga atatcaagtt taccactgca tctaaatcag catctcttag
     1201 tttagttaaa tccacttagc aaaaagcgtt aacgattcac tttctgactt aggcacctcg
     1261 ttattccaca aagtagcgca ttctttctga taataccatc ccgcaaaagt gtatccttta
     1321 cgaacagggt cagaaggtat gacataagga ttactaccat taatataatc cataaagtaa
     1381 tagccttcat ttagggaaga ttcaaaatta tacatgtaga aagtattaag tttatttttc
     1441 ccgtttagat aatgtaattt aatttcagga aatgaattaa tagttaatac cttatcaaag
     1501 tcatctagat tagccaaagc aaggatagct tcgacagtcc tcactgacgc taaccacatt
     1561 tgcccacgca aggaatatgg aagataaatc tttatttgct tatctgttaa cttcagtgca
     1621 ttaatatcta agggttgaaa aaatcgtttt ggttttccta tataccgaac tggaaaacct
     1681 ctaatgcttg gaggcacaac caaaacttct tgttcttttc cggcctcggt taattcaatt
     1741 gccgttgcat atttatctct gtgattgatt tcaaatataa aatggtcgtt tgccatgaat
     1801 tctttcttgg gtatacttgc ttttaatcct agcgcaagta acatgactaa acttacgtaa
     1861 gcaattttat tagtattaat ccgtctgcac atagtttctc ccttacgcta aagaatagcg
     1921 tcaccatttg atcgaataat cattgaccac tacatataaa caagtgactt aaaaaatata
     1981 atgtaatttt atcatgagtt ttatatacgc tatcctcttt ttataaatca cattttcatc
     2041 atctttaact actgtatatt atttttcgac ctcaaaagtc gctatttaaa aagggaacca
     2101 ttttcttggt cccctgtaat ttgctttatg tagataacac tacatattat taataagttc
     2161 agcgtaactt tcaacactaa gcgaggcacc gccaacgagg gcaccatcaa tatttggcat
     2221 cgccatatat tctttaatgt tgttaggttt aactgagccg ccgtaaagaa ttcgtactaa
     2281 atcagcgtca ccgccgtatt tagcgcgtaa taattcacgg atataagcgc agatgtcttc
     2341 tgcaatttct ttacttgcat ttttaccagt gccaattgac cacactggtt cgtaagcaat
     2401 cacgacttct ttaacgtctt taacaccttt aagccccact tcaatttgtg ctttaattac
     2461 ttcttttgtc acaccttttt caaattcttg taatgtttca ccaacacagt atagtggtgt
     2521 catattattt tcaagtaaag cgaggacttt taagttacag ctttcatttg tttcgttaaa
     2581 gtacgtcctt ctttcactgt gaccgacaag tgaccaacta acacctagtt cttttagcat
     2641 tggaatacta atttcaccag tgtaagcccc tttttcaagg tgatgaacat tttgcgcacc
     2701 aataatgagc tttggattaa tatttaataa ggtttgcagt gataaataag gtggggtgac
     2761 tccaatatca atatttttag ctttggcatt tttaagtaat tcaaaggaag catgagcgaa
     2821 aactgctgtt tccgccattg ttttattcat cttccagttg ccaacaagta atttcttccg
     2881 catattatcc aatcacatca atgcctggta aatgaccatc attttcaatc atttctagtg
     2941 atgctccgcc acccgtgctt acatggctaa agtaatcctt ataaccaaat tgcgcgacgg
     3001 cggctgcact atcaccacca ccaatgacag tgaatgctcc ttttaattta ctcacagctt
     3061 cgcacacagc gcgtgtacca gcttcaaaat ctttttgttc aaagacaccc attggtccgt
     3121 tccagaaaat cattttggct ttgcttaatt catccgcaaa taatttttgt gtttttggcc
     3181 caatatccat cccttcaaaa ccgtcaggaa tattcgaatc cgttgtcgtt attttctttg
     3241 tccagtcttg gaaggcatca gtggcaacag cgtcaactgg taataaaatc ttctcacaag
     3301 cagtgtctaa acatttcttt gcaaagtcga cttgttcagg ttcaaacggt gaaatcccga
     3361 tattatgacc ttgagcgact tggaaagtgt aagccattgc cccaccaatt aatactttgt
     3421 cacatttttg taataaagtt tcaataactt taattttgtc actcacttta aagccaccaa
     3481 taacggcaat ataaggccgg tcttttttag cgacatgaac aacacggcca agtgcttcga
     3541 cttctttttc gactaaaaag ccaagcgcaa cttctttacc gcgggctttt aagagttcag
     3601 gaacaccgac agtcgaagcg tgagcgcggt gggcactacc aaaagcgtcc atcacgaaga
     3661 catcagcgaa acttgcccac ttttcactaa gaacggggtc atttttctct tcaccttttt
     3721 cgtaacgcgt attttgtaag agtaagactt gtcctggttt aagtaaagca acacgcttat
     3781 ttacttcttc gccactagtt tcagggctaa agaagacttt atcttcacct aaaatttccg
     3841 ctagacggcg gaagacaatt accatattat ttttcttttt agcggcttca acttcttctt
     3901 cggttccttt atgtttaatt ttaccaaggt gtgagaaaag cactactttt gcgccagaat
     3961 caagcgcaaa cttaattgta ggtaaagcag ctctaatacg gttatcatca ctgatgactt
     4021 cgcctttaaa tggcacgtta aagtcacagc gaattaacac tgttttgttt gctaatttaa
     4081 gatctctaat aattttagcc atattttttc tcctttttta gataaaaagc gcccgaaggc
     4141 gctttcttta aatcattaat aaattattta acgccgtgaa ctttggcaac ttcttgcgca
     4201 agacggatgt attggcaagt atagccatat tcattgtcat accagaacat tactttaacg
     4261 agttgttcac catcgacttc cataactttc gttaaggttg cgtcaaataa accaccgtgg
     4321 gtatcaccaa tgatgtcgcc actgacgact tcgtcttcgg tgtaaccaag aatggctttt
     4381 aattcacctt cagcagcttt tttcatcaca gcgttaattt cttcagcagt aacttttttc
     4441 tttaagagca ctgttaagtc aaccattgaa ccatcgatta ctgggacacg taaagcacca
     4501 ccatcaagct ttttgttaag atgtgggagt acttgaccaa ttgctttagc ggcaccggtt
     4561 gaagttggaa cgatattcat cgcacctgcc cgaccacgac gtgcagcttt tttctttggt
     4621 aagtctaaga ttgtttggtc atttgtatag gcgtgaacag tagtcattaa accgcgttcg
     4681 acaccaaaat tgtcatcaag cactttcacg actggcgcta gtgcgtttgt ggtacagctt
     4741 gcaccactaa tgacggtcat atcttttgtt aatttatcgg tattaacacc gtaaacaaac
     4801 attggggttt ctttatcagc aggagcacta atgatgacgg ctttcacacc ttcttgtaag
     4861 tgcacacttg catcttcttt accacggaaa cgaccagtac attctaagac gatatcaaca
     4921 ccgtgttttg tccaatttaa ttgggttgga tcaggttgag cgtaaactgg gatttccttt
     4981 cctttaaaaa cgattgctgt tccatcagcg gtcacgctaa tgtcatcaac gtggaaacga
     5041 ccgtgtgcgc tatcatattt taaaaggtat gctaaatttt cagcatcagt taagtcgtta
     5101 atcgcgacaa cttccattcc ttttgtttcg gcaatccgac ggaatgctaa gcgaccaatc
     5161 cggccaaatc cattaattgc aattttaatt gacataattt tcctccttta tttatgcgtt
     5221 tatattatac aacattagtt atctaatgta ctaacaaaaa catacaaaaa aacgaatatt
     5281 ttcacttat
//
LOCUS       DAOSTA010000053         5186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_14596_length_5186_cov_7.990450, whole genome shotgun sequence.
ACCESSION   DAOSTA010000053 DAOSTA010000000
VERSION     DAOSTA010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14596_length_5186_cov_7.990450"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PLU08_04575"
     CDS             complement(<1..945)
                     /locus_tag="PLU08_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ70997.1"
                     /translation="MYDLLTQTIYSAIPLAIVAMGGMYSERSGVVNIALEGTMMFGAF
                     VGILCLSHIQHAGIDGQHIIFLALLIGAISGVIFSALHAFASITMKSDQTISGTALNM
                     LAAGLAIFMGRALSLGRTEEITFSSTHHRISAIPLLRHIPFFGKILFEDIYLISLFGI
                     LLFIVMGVIAYKTKFGLRLRACGENPHAADAAGINVIRTRYIAVLISGMLGGIGGVLL
                     IIPTTVSFIANVYGFGFLALAVLISGQWKPSRIMIFAIFFGLLRSIAFGFAFIKADLS
                     HIAFLKTFLDKLPTDILNMLPYVVTLIVLAFTSLKSRGP"
     gene            complement(1061..2293)
                     /locus_tag="PLU08_04580"
     CDS             complement(1061..2293)
                     /locus_tag="PLU08_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ70998.1"
                     /translation="MTEVTKKRTCSLKFKTWWRKVVESTGIEKFLATIIAIIAGLLFG
                     LIVMLVINITDPFVAFEGLGAILTAGFSKGVKSIGEVLFRAAPLILVGLSVAFAFKTG
                     LFNIGASGQLMMGSFLAVFVGIKTESWILALISGVLGGALWALIPALLKAYRNVHEVV
                     ATIMMNYIAMYSSKMLIEWLIFMPIQQQARPVPTGGQLPTLGLDKLFNFPRLNSGIVI
                     AIITTIIIFVLLEKTTFGYQLKAVGFNREAARYAGINDKRNIVLSLVIAGALAGLAGA
                     VIYLVPAQGERLATKHVISNYGFQGIAVALLGMSHPIGTMGAGLFFGYIEAANLPLQR
                     VGFKREIIDIISASIIYFSAFTLFFQIYARRFINKMRDFFAKIKKKPQLATAEGVEIE
                     TLPPNEEGKEGGQEDDRK"
     gene            complement(2277..3812)
                     /locus_tag="PLU08_04585"
     CDS             complement(2277..3812)
                     /locus_tag="PLU08_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013244738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ70999.1"
                     /translation="MSYVIEMLNITKSFPGIKANDNVTLQLKHGEIHALLGENGAGKS
                     TLMSILFGLYQPDEGTIKVHGEVVNIKNPNDANDLKIGMVHQHFKLVEDYTVLENIIL
                     GREETKNGFLTTKNARKKILEIADHYGLHIDLDAKIEDITVGMQQRVEILKMLYRDAE
                     ILIFDEPTAVLTPQEIVELMDNMRDLINEGKSIILITHKLNEIKMVADRCTVLRKGQY
                     IGTVDVKNTTVEEMAEMMVGRKVSFHIEREKSEPGEVILEVKDVNHFNDYLNKNDLTD
                     VSFNVRAGEIVVVAGIEGNGQSQLASVITGLRNVKSGNIYLNGDEITRKTIRQKTLTG
                     ISHIPEDRHKHGLVLDFNLAYNLILQTYFTPEIQKYGFLKFDSINKKADELIERFDIR
                     SGEGRKTPMRSMSGGNQQKTIIARELSRPMDLVVAVQPTRGLDVGAIEYIHKQLIKLR
                     DERKAILVFSLELDEVFAIADRILVMFEGRIVANLITAETNENELGLYMSGSKEATYD
                     GSN"
     gene            complement(3930..5048)
                     /locus_tag="PLU08_04590"
     CDS             complement(3930..5048)
                     /locus_tag="PLU08_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012993634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOQ71000.1"
                     /translation="MKKSRLLAGFVLLAGIMLSVTGCREKTYKLALVTDVGTINDKSF
                     NQGAWEGLEKYAKDKKMTYQYYQPKQQTTNDYVESAKVAIENGAEVVVTPGYLFENAI
                     WQLQTEHPDVKFILLDGSPHNVIDWDRMATLDGEDPNYDIKDNVYSIFYAEEEAGFYA
                     GYASVKEGMRDLGFMGGMAVPAVVRFGMGYIEGAKYAANELELDDGAVTMKYTYLNSF
                     IPDAAHQTKAAAWYGTGTEVIFAAAGGAGGSVMKAAEEKQNKWVIGVDVDQRAESRTV
                     LTSAIKSLGSSVYQTLDKIYKDGVKGGTSETLDSTVGGVGLPAKSDADFDRFQNFNKA
                     QYDAVYELVKNGTITVYDDVEDPDYVALGGAKVVVTVE"
BASE COUNT         1603 a         1105 c          902 g         1576 t
ORIGIN      
        1 tggtccgcgt gatttaagtg atgtaaaggc aagcacaata agtgttacaa catatggcaa
       61 catatttaaa atatccgtcg gtaacttatc taagaatgtt ttaaggaacg caatatgact
      121 taagtctgcc ttaataaaag caaaaccaaa agcaatactc cgtaagagtc caaagaaaat
      181 cgcaaaaatc ataatccgac ttggtttcca ttgaccactg ataagaacgg caagtgctaa
      241 gaacccaaat ccatacacat tcgcgataaa actcacggtc gtcgggataa taagtaagac
      301 accgccaata ccgccgagca ttccgctgat taaaacagca atataacgag ttctgatgac
      361 attaattccg gctgcatctg cagcatgcgg attttcgcca catgctcgta agcgtaatcc
      421 aaacttcgtc ttataggcaa taacacccat cacaataaag agcaaaatac cgaataatga
      481 aattaagtaa atatcttcaa acaggatttt accaaagaac ggaatatggc gtaaaagcgg
      541 aatcgcacta atacgatgat gcgtacttga aaaggtaatt tcttctgttc ggcctagtga
      601 aagtgcgcgg cccataaaga ttgctaaccc agcagcgagc atattaagtg ctgtccccga
      661 aattgtttga tcacttttca ttgtaattga ggcaaaagcg tgtaaggcgg agaatataac
      721 gccagaaata gccccaatta agagcgcaag gaaaataata tgttggccat caattcccgc
      781 atgttggata tgacttaagc ataatattcc gacaaatgcc ccaaacatca ttgtcccttc
      841 aagggcaata ttaacgacgc ctgaacgctc actatacatg ccgcccatcg ccacaattgc
      901 taaggggatt gccgagtaaa tcgtctgtgt taaaagatcg tacatattat cactcgtaaa
      961 gatagcaagt aacacataaa ggacaagtaa aactgcgatt gtaataattt gttttttcga
     1021 aagtttcgga agtttagcaa gaaaaacttt gaactttgat ttattttcgg tcatcttctt
     1081 gtcctccttc tttaccttct tcattcggtg gtagcgtttc aatttctact ccttcggcag
     1141 ttgctaattg tggttttttc tttatcttag cgaagaaatc acgcatttta ttaataaagc
     1201 gtcgtgcgta aatttggaag aataacgtaa aggcactaaa gtaaataata cttgccgaaa
     1261 taatatcaat aatttcgcgt ttaaacccaa ctctttggag tggtaagttc gccgcttcaa
     1321 tataaccaaa gaagagcccc gctcccatcg ttcctattgg atggctcatc ccaaggagtg
     1381 caacggcaat cccttggaag ccgtaatttg agataacgtg ttttgtggca agtctttcgc
     1441 cttgcgctgg cactaagtaa ataacagccc ccgctaaacc agcaagagcg ccagcaatta
     1501 caagtgagag cacgatatta cgtttatcat taatccctgc ataacgcgcg gcttcacggt
     1561 taaagccaac tgctttaagc tgataaccaa aggttgtttt ttcaagtaac acaaaaatga
     1621 taattgtcgt aattatcgct attactatcc cgctattaag gcgcggaaag ttaaataatt
     1681 tatcaagtcc taatgttggt aattgtccgc ctgtgggcac aggacgtgct tgttgctgaa
     1741 ttggcataaa aattaaccat tcaattaaca tctttgaaga gtacattgca atatagttca
     1801 tcataatggt cgccacaact tcatggacat tgcgataagc tttaagtaag gctgggatta
     1861 atgcccataa tgcgccaccg agcacaccgc taataagcgc taaaatccat gattcggttt
     1921 taatccccac aaaaacagct aaaaacgacc ccatcattaa ttgtccacta gcgccaatat
     1981 taaataagcc tgtcttaaag gcaaatgcga ctgaaagccc cactaaaatt aatggtgcag
     2041 cgcggaagag cacttcacca atgcttttta cgcctttaga gaaaccagca gttaaaatcg
     2101 ccccaagtcc ttcaaaagcg acaaatggat cagtaatatt gatgacgagc ataacgataa
     2161 ggccaaatag aagcccagcg ataatcgcaa taatcgtcgc aagaaatttc tcaatccctg
     2221 tggattcaac aactttgcgc caccaggttt taaattttag actacatgtc cttttcttag
     2281 ttacttccgt cataagttgc ctctttcgaa cctgacatat aaagcccaag ttcattttca
     2341 ttagtttctg ctgtaattaa attagcgaca attcgtcctt caaacatcac taaaatgcgg
     2401 tccgcaatgg cgaagacttc atctagttca agggagaaaa caagaattgc tttgcgttca
     2461 tcacgtaatt tgattagttg tttatgaatg tattcaatcg cccccacatc aagtccgcgt
     2521 gttggttgga cagcgacgac taaatccata ggcctagata attcacgagc gataattgtt
     2581 ttttgctgat taccgccact catactacgc attggcgtct ttctaccttc accactacga
     2641 atatcgaagc gttcaattag ttcatcggcc tttttattaa tactatcaaa ttttaagaaa
     2701 ccgtattttt gtatttcagg agtgaaatat gtttgtaaaa taaggttata cgctaaatta
     2761 aaatctaaaa caagtccgtg tttatggcga tcttctggga tatggctaat acccgttagt
     2821 gttttttggc gaattgtttt acgtgtaatc tcatcaccat ttaagtaaat gttccctgac
     2881 ttaacattcc gcaacccagt aatcacactt gcgagttgac tttgaccatt accttcaatc
     2941 cccgctacga cgacaatttc tccagcgcgc acattaaagg agacatctgt taagtcattt
     3001 ttatttaaat aatcattaaa gtgattaaca tcttttactt caaggataac ttcaccaggt
     3061 tcacttttct cgcgttcaat gtggaaacta actttgcgtc caaccatcat ctctgccatt
     3121 tcttcaactg ttgtgttttt tacatcaact gtcccaatgt attgtccttt ccgcagtact
     3181 gtacagcgat cagcaaccat tttaatttca ttaagtttat gcgtaataag aataattgac
     3241 ttaccttcat taattaagtc acgcatatta tccattaact ccacaatttc ttgcggggta
     3301 agcactgctg ttggttcgtc aaaaattaaa atttccgcat cacgataaag catctttaaa
     3361 atctccacac gttgttgcat accaacagta atatcttcaa tctttgcgtc taaatcaatg
     3421 tgtaaaccat agtggtcagc aatttctaag atctttttac gtgcattttt ggttgttaga
     3481 aaaccgtttt tcgtctcttc tcgaccaaga ataatatttt ctaacacagt ataatcttcc
     3541 actaacttaa aatgttgatg gaccatccca atctttaaat cattagcgtc atttggattt
     3601 ttaatgttga caacttcacc atgcacttta attgttcctt catcaggttg atatagccca
     3661 aaaagaatcg acatcaatgt acttttccct gcaccgtttt ctcctaataa agcatgaatt
     3721 tctccgtgct ttaattgcag cgtaacattg tcattagctt taatgccagg gaagctcttt
     3781 gttatgttca acatttcaat aacgtaggac ataataaaat ctcctttaca actttaaatc
     3841 aattataaca tgaagttttt aatttggtaa taaagtaaaa ggccgcgcgt ataattgcgc
     3901 ggcctttagt tagcttatgt taaaaattat tattcaacag taacaacaac ttttgcgcca
     3961 cctaaggcaa cataatctgg atcttcaaca tcgtcataaa cagtaatggt accattttta
     4021 acgagttcat aaacggcatc atattgtgct ttgttaaagt tttggaagcg atcaaaatca
     4081 gcatccgatt ttgctggtaa accaacaccg ccaacggttg agtctaaagt ttcacttgta
     4141 ccacctttaa cgccatcttt atagattttg tctaaggttt ggtacacact tgagcctaaa
     4201 ctcttaatag cggatgttaa gactgttctt gattctgctc tttggtcaac gtcaacacca
     4261 ataacccatt tattttgttt ttcttcggca gctttcatca cactaccacc agctccacca
     4321 gcagcggcga agataacttc ggtcccagtc ccgtaccacg cagcagcttt agtttggtga
     4381 gcagcgtcag gaataaagga gttaaggtat gtgtatttca tagtgacagc accatcgtct
     4441 aattctaatt cattagcagc gtattttgcc ccttcaatat aacccatacc gaagcggaca
     4501 actgctggaa cggccatacc gcccatgaat cctaaatctc tcatgccttc tttaactgaa
     4561 gcatagccag catagaagcc tgcttcttct tcggcataga aaattgaata cacgttatct
     4621 ttaatgtcat aatttgggtc ctcaccatca agtgttgcca ttctatccca gtcaatgacg
     4681 ttatgtggag aaccatcaag taagatgaat ttcacatctg ggtgttcagt ttgtaattgc
     4741 caaatcgcat tttcgaataa gtagcctgga gttacaacaa cttccgcgcc gttttcaatg
     4801 gcgaccttag cagattcaac gtagtcattc gtggtttgtt gttttggttg gtaatactga
     4861 taagtcattt tcttatcttt cgcgtatttt tccaatcctt cccacgcacc ttggttgaat
     4921 gatttgtcat tgattgtacc aacgtcagta acgagcgcta gtttgtaggt tttttctcga
     4981 caaccagtga cagagagcat aatgccagca agtaatacaa aaccggcaag tagacgagat
     5041 tttttcatta aagttcctcc ttattatgtt atatgtggtc tacatattta gcatatcata
     5101 aaaaaagtgt ttaacaataa aaatgtttta gaataataga agaaaaggtt aaattgacaa
     5161 gataaaaaac tgaaagaatc attttt
//
LOCUS       DAOSTA010000054         5013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_15227_length_5013_cov_9.527632, whole genome shotgun sequence.
ACCESSION   DAOSTA010000054 DAOSTA010000000
VERSION     DAOSTA010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15227_length_5013_cov_9.527632"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PLU08_04595"
     CDS             complement(<1..865)
                     /locus_tag="PLU08_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOQ71001.1"
                     /translation="MKKFSIVVNSSVDLTEELRKELDLVVAPLKFIMNGQEYINYLDY
                     RNLGFKEFYQKLRDGVVATTSQVNINEYVDIIEKEFKKGNDVLILSLSSALSSNYNSA
                     RLAVDMFKDHPEHKVLLVDTLSASLGEGLIAYAASMKRKEGATLEETYQFIEQFKYNV
                     STWFTVDDLMFLKRGGRLGGVSAIFGTLLAIKPILHVDLEGRLIPMEKTRGRKKALRI
                     IADKVKETYDPSLPNFCYVAHSDDEEAANLLKDMIEGLGIGLKVTLVNYVGPVIGAHV
                     GPGTIGAFFFAK"
     gene            complement(1014..1760)
                     /locus_tag="PLU08_04600"
     CDS             complement(1014..1760)
                     /locus_tag="PLU08_04600"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOQ71002.1"
                     /translation="MKNNFKTVLIKALKIIAIFVGYAVLAALIYLFFYLNGYKSLDDI
                     RGLIDNAGAWAWLIFSIIQIFSTVVLFIIPAQTTAFIVLALALFTPLEAFLLVAINTL
                     IASLLNFSVGKLFGKVIVQKIIGEETIIKYQEKLETKAKIYYPIMMLFPFFPDDEITM
                     LAGLTPMTYRYFIPVTFVTRSVGIATYVFLGSTFNYQNFTVLHWYQFIVIIGLLLYLV
                     FFLADKLEKYLDSTPLSNFSFWRWSTRRKR"
     gene            complement(2113..2592)
                     /locus_tag="PLU08_04605"
     CDS             complement(2113..2592)
                     /locus_tag="PLU08_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ71003.1"
                     /translation="MLLNTCATTSPWLRDIGESWNKAGATGDGHAYYLSTIPLIYSPA
                     FYTKKLDWMKKREALLTPFFSNKVVVDGFIRLTNSAVSHDVRDSLSKIDVPTFIISAK
                     QDYLTPIEDQYLLAEKMPNVSHITIENSGHASMYEHPGLFVTLVLGFVNKKAKTYTI"
     gene            complement(3335..3988)
                     /locus_tag="PLU08_04610"
     CDS             complement(3335..3988)
                     /locus_tag="PLU08_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71004.1"
                     /translation="MKPLIVICYCSVSNNKYITELKSSKVSHSTTKYKREVWQCNKKF
                     ENGKKGNKCSTPHLFEREIKEAFIKAFNLLCQKKNSVLNNCKDFITILNNTKELDEKI
                     MVQETEVKVLINIARNLVEENATTALDQEDYKNRYAKIEKKFKEEQDKLDELLKERER
                     KRVQKNSIKLFMKAYKEMPEILKEWSLEVWITIIDHAIVYSDGKMKFIFKSGDEIKV"
     gene            complement(3948..4871)
                     /locus_tag="PLU08_04615"
     CDS             complement(3948..4871)
                     /locus_tag="PLU08_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOQ71005.1"
                     /translation="MKYEKISYFVSKWGITERRIRVLCTEGRIEGAVKVGKTWLIPKD
                     ASKPRDRRYKNINDLFFDDINTDAIDNKKAIIDESRPFSQTMIRQLNDKLLVEWTYHS
                     NAIEGNTLTMSETKVVLENGITIKGKPLKDHLEAINHKEAIEYVEELVKKDTKLTEYD
                     IKSIHYLILKEIDNLNAGKYRAQNVLISGSKHIPPSYINVPLEMQMLMEQYEDWAVYH
                     PLIRACLLHGEFVKIHPFVDGNGRTARLLLNFELIKNGYPPVIIKNEDRADYYDALEM
                     ASTTKNYTLFINLVLNLVNEAADSYLLLLGK"
BASE COUNT         1664 a          899 c          721 g         1729 t
ORIGIN      
        1 gttttgcaaa gaagaatgca ccaatcgtgc ctgggccgac atgcgcccca ataactggtc
       61 caacataatt tactaaagta acctttaaac caattcctaa gccttcaatc atgtctttta
      121 ataaattcgc cgcttcttcg tcatcactat gcgcaacata acagaaatta gggagacttg
      181 ggtcataagt ttctttaact ttatcggcaa tgatgcgtaa agctttttta cgaccgcgtg
      241 ttttttccat tggaatgaga cgcccttcaa ggtcaacgtg taagattggt ttaattgcta
      301 aaagtgtacc aaatatagca gacactccac ctaaacgacc accacgcttt aaaaacatta
      361 aatcatcaac cgtaaaccac gttgagacat tgtatttaaa ttgttcaata aattgataag
      421 tttcttcaag tgtcgctcct tctttccgtt tcattgaggc agcgtaagca attaaacctt
      481 cccccagtga agcacttaat gtatcaacaa gtaatacttt atgttcagga tgatccttaa
      541 acatatcaac agcgagacgt gcactattat agttgcttga aagtgcactt gaaagtgaaa
      601 gaattaaaac atcattacct ttcttaaact ctttttcaat aatatcaaca tattcattaa
      661 tgttaacttg cgatgtcgtt gcgacaacac cgtcacgcaa cttttgataa aattctttga
      721 atcctaaatt acgatagtct aaatagttaa tatactcttg accattcata ataaacttaa
      781 gcggagcaac tactaagtct aactctttcc gtaactcttc agttaagtca acactagagt
      841 taacaacaat actaaatttt ttcataaata aatttctcct tttgtagcgc tattaatatt
      901 taataaacat ctaattatat acgagaaaaa atttgaaagt ccacaaaagt gaatattttt
      961 cataattgtt cattacgcaa attataaaag cttattttaa tacgtgtata tatttagcgt
     1021 tttcttcgtg ttgaccaccg ccaaaaagaa aaattactaa gtggcgtgga atcaagatat
     1081 ttttcaagtt tatccgcaag gaaaaaaaca agatataaaa ggagcccaat aattacgata
     1141 aattgatacc agtggagcac cgtaaagttt tgataattaa aagtactacc taagaaaaca
     1201 taggtcgcaa ttcccactga acgcgtcaca aaagtcacgg ggataaaata gcgataagtc
     1261 atcggagtaa gaccagcaag catcgttatt tcatcatcag gaaaaaacgg aaaaagcatc
     1321 ataattggat aataaatttt tgcttttgtc tcaagttttt cttgatattt aatgattgtt
     1381 tcttcaccaa taattttctg tacaattact ttgccaaata acttaccaac agagaaatta
     1441 agtagcgacg ctataagtgt attaatcgct actaaaagaa aagcttcaag cggagtaaaa
     1501 agcgctaagg ctaagacaat aaaggccgtc gtttgtgcgg gaataataaa aagcacgacc
     1561 gttgaaaaga tttgaataat tgaaaagatt aaccacgccc atgctcccgc attatcaata
     1621 agtccgcgaa tatcgtctaa acttttataa ccatttaagt aaaaaaatag ataaattaaa
     1681 gctgctaaaa cagcatagcc aacaaatata gcaataatct ttaaggcttt aattaaaact
     1741 gttttaaaat tatttttcat gactattacc gccaacatta taacgaattt aagtgaacat
     1801 tacttaaaaa tagatgttaa taataaatta aatatgttta taacaaaatc cacatcccga
     1861 caattaacaa acagaaaagc aataatataa aaacatatct tgctaataat cgcattttat
     1921 cttttgaata attatcaact tttttacaaa gtgcgaaata caagaaaaaa agccatttta
     1981 acccgtaata ggtttatatg gctttaaggc aaatatgcgc cttttaatta ttagaatatt
     2041 atacttaaaa gttttgcacg ttactggttg tttttgcaaa cagtaacgct aaataaattt
     2101 atgcgttagt ggttaaatcg tatatgtttt tgctttttta tttacgaagc caaggactag
     2161 cgtcacaaat agaccaggat gctcatacat actagcgtga cccgaatttt caatcgtgat
     2221 atggctcaca ttcggcattt tttcggcgag caagtattga tcttcaattg gggttaaata
     2281 gtcttgcttt gcgcttataa taaatgttgg tacatcgatt ttacttaagc tatctcttac
     2341 atcgtgacta acagcgctat tcgttaagcg aataaagcca tcaacaacaa ctttattact
     2401 gaaaaatggt gttaagagtg cttctctttt tttcatccag tcaagtttct tcgtgtagaa
     2461 agctggtgaa taaattaacg gaatcgttga taagtaataa gcgtggccat ctcccgttgc
     2521 ccctgcttta ttccacgatt caccaatgtc tcttagccat gggctcgttg tcgcacatgt
     2581 attaagtaac ataagacttt caacataatc ttaatattta accgatatcc ataaaagtca
     2641 ttaattactc atcgtaatga aatagcatct agttaaaatg tattagaaat aaaaaagtca
     2701 gaaggtcaaa tttctgactt tttattatgg cgtaaaagaa aatttaaaga ttaatcatca
     2761 tctttatcat atttaaattt gatgttttgt aaaaggttta ttccggtttt ttcagaggtt
     2821 tcgctccgtg tttttcagaa gcttgattcc agtttttgca gatttatgaa ttttttctat
     2881 tataatatct tcccttgcct caacacttct aatcgttcag atagtggaat tctagataat
     2941 taaaattact cccttcaata acctttaaaa tgtcaaatta ctatcaatta tcgaaaaaat
     3001 gtattatttt tctatctttt tctaataaaa aatgccttga aattgtatca aggcatacct
     3061 agtaagatgg catgttgtcc taattgatac aatttcggaa gtaatcgttc aatcatgcac
     3121 cccccaccct tcaaatcgtt caaatgtgca accccctagg ttataatcgt tcagacaata
     3181 ggattccgaa aaaaggaaaa gagattcgct ctccagtgta aataatggtt tggtactatt
     3241 taatatttaa aaatgctaac tataaattac attcttgaca agttcttaaa gtccctgttt
     3301 tgttaaccat atgaaacata tttaatttat aaacttatac tttaatctca tcaccacttt
     3361 taaagataaa cttcatctta ccatcactat atacaatagc gtggtcaatt attgttatcc
     3421 atacttcaag agaccactct ttcaatattt cgggcatttc tttataagct ttcatgaata
     3481 acttaatact attcttttgc actcttttac gctctctttc ctttagtaat tcatcaagtt
     3541 tatcttgttc ttctttaaac ttcttctcga ttttagcgta tcgattttta taatcttctt
     3601 ggtctaatgc ggtagtggcg ttttcttcca ctaagttacg agcgatatta ataaggactt
     3661 taacttctgt ctcttgaacc attatttttt catctagttc ttttgtatta ttaagtattg
     3721 tgatgaagtc tttacaatta tttaaaacgc tattcttctt ttgacaaagt aagttaaagg
     3781 ctttaataaa tgcttcttta atctctcttt caaataaatg aggagtacta catttattcc
     3841 cttttttacc attttcaaat ttcttattac actgccacac ttctctttta tatttagttg
     3901 ttgaatgcga aacttttgaa ctcttgagtt cggtgatgta cttattatta cttaccgagc
     3961 aataacaaat aactatcagc ggcttcatta actaaattaa gcacaagatt aataaaaaga
     4021 gtgtaatttt tcgtggtact agccatttca agagcgtcat aataatccgc gcgatcttca
     4081 tttttaataa tcacaggtgg atagccattt ttaattagtt caaaatttaa aagtaagcgc
     4141 gcagttcgcc cattaccatc aacaaatgga tgaattttca caaattcacc gtgcagtaaa
     4201 caggcgcgaa taagcggatg ataaactgcc caatcttcat attgttccat taacatttgc
     4261 atttcaagcg gcacattaat ataggagggc ggaatatgtt ttgaaccact tattaaaacg
     4321 ttttgggcgc gatatttgcc agcatttaaa ttatcaatct cttttaaaat taaataatga
     4381 attgatttta tgtcgtattc tgtgagttta gtgtctttct tcactagttc ttcaacatat
     4441 tcaattgctt ctttatgatt gattgcttca agatggtctt ttaatggttt gcctttaatt
     4501 gtaatcccgt tttctagcac aactttcgtt tctgacatcg ttaaagtatt gccttcaatc
     4561 gcatttgagt gatatgtcca ctcgacaaga agcttgtcat ttaattgtcg gatcatcgtt
     4621 tgtgaaaaag gtcgtgattc atctattatt gcttttttat tatcaatagc atcagtattt
     4681 atatcatcaa aaaataagtc attgatattt ttataacgtc tatcacgtgg ttttgatgca
     4741 tctttgggaa ttaaccatgt tttgccaact tttactgcgc cctcaattct tccttcggta
     4801 caaagcacgc gtattcgccg ttctgtaata ccccactttg agacaaaata cgatattttt
     4861 tcatatttca tataatcacc tccaatgtta ttataccgat atcggcacaa taatattata
     4921 gcatttcaaa gaaataagac aacattatac cgatatcgga ataataaata tgcgtactca
     4981 taataaaaaa tgcctcgata attgtatcaa agc
//
LOCUS       DAOSTA010000055         4939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_15505_length_4939_cov_8.815930, whole genome shotgun sequence.
ACCESSION   DAOSTA010000055 DAOSTA010000000
VERSION     DAOSTA010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15505_length_4939_cov_8.815930"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..2423
                     /locus_tag="PLU08_04620"
     CDS             117..2423
                     /locus_tag="PLU08_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOQ71006.1"
                     /translation="MDIFTLLEFNEIVSKVKDLTKTELGGEYASLISPFESEEEATYA
                     LNLVSEAESILTRFGALPLAPSHKLAPLLEEARRGNILTPHDFYLISADAMNIVKINY
                     YLKDKLDNAPLLKAMLKREKDLAVLANEINKVITPNLLVKDNASHELARIRKNIALSE
                     DRLNKAAQGLISKYGNFLAENLITKRDGHFVIPVKTAYKNKVDGIIYDISNSGQTIFV
                     EPSEIAALNSTLISYQNDEIIEVNRILRALTLETLKYEDEIINNNILLAELDLIFAKA
                     TYGQSINGFVPVFQQTKGLQFKKARHPLIDKNLVVANTFNLKDDKFIIVISGPNAGGK
                     TVALKTMGLLVLMAKSGLMLPVSEEPKLFFYKNVYASIGDEQSLSQNLSTFSSHVKRI
                     GEIMDVATSDDLVLLDELATGTSPEEGEALALAIATYLLNNSISSVISSHYSRLKEFA
                     YSRSGIINASMAFDEKKLTPLYEYHEEIPGRSYGLVVAKRFGIKDEVIKDAESFLSDG
                     VYDFESTIGKLRNEIRTLELERTELEKERAALLKKEAELDKKLKRQSEAEEDFKLTKN
                     ERLEKEINEAIATLDTLVKSALASGAKAHEIIAVRDEIKKLGKESKKEVKAPAKDFEL
                     GDYVEVKKLHVKGEITRIRGEEYTVTLASGKILKVSASDLEITERTKRKSTPTITVKG
                     LEEDLTSVKPEINVIGMRVEEALNVIGPYLDRALLKRYPSVRIIHGFGTGALRRGIHD
                     YLAKLSFVKSYTLAGEYEGQGGATIVTF"
     gene            2424..4199
                     /gene="uvrC"
                     /locus_tag="PLU08_04625"
     CDS             2424..4199
                     /gene="uvrC"
                     /locus_tag="PLU08_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOQ71007.1"
                     /translation="MKNKKLQFLIDHLPSDPGVYLMLDSHGRIIYIGKAKDLKSRVSQ
                     YFTRPQPPKVAAMVKRTAKFETIVTNSEKEAFILELKLIQTHYPRYNIMLKDDSHYPY
                     IALTKKGDPVVSIKRKTSDDKYIYFGPFPASQKAYQIVDLINKIFMTRKCKGKSKDPC
                     FYFHLGQCPGYCFKDISDLEKDKVRSDILTFLNGKDKDTLKRYETKMKIASAELDFEK
                     ARDYKEIVDAILHIYSSQNIELKNKVDLDVIAFTQINDYIGLNIVSYRAGVLLNEQFD
                     AFPSFSDLHEQIINLIGQYYTKNPLPTKLMVGNSFIAEALTLYYDKEVIVPSRGQNLE
                     ILNRAYQNATSALQKHLLYHQALGTENESLTALSETLGIKYPSHIDFVDIAHLFGSNA
                     QGVVITYINGRPFKKLYRKYNIENENSFDDYASIKEVLTRHYKRKLIEGKHLPDLLIV
                     DGGKGQLGVALSVKDELNLDFNIISLVKDDKHTTKALINEEGDEISVNKNLLTFLAAI
                     QNEGHRYAITSHRAKRGKGTYTSALDGIKGLGKKRKMLLLNTYKSIDVLKSVPLTELS
                     QLIPREVAEALLTKLNRVDDTEGGV"
     gene            4201..>4939
                     /locus_tag="PLU08_04630"
     CDS             4201..>4939
                     /locus_tag="PLU08_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71008.1"
                     /translation="MTKVKTKKGPKIILAVASLLVVTILSLSIVRIVKNNPPKHKDYA
                     IYLYGEEHGVAKINKKELALWDDFYHKQGMRHLFIESAYFDAAILNLWMQEEDDTRFE
                     YLYDGWEGTLSHNKETRDFYFAFKESYPETIFHGTDVGHQHERAGQYYLDYLIDNGLE
                     NTEAYTLTLESIAQGEEFTKTGDLKYRESKMVSNFIREFDALDNEVIMGIYGSAHISK
                     DFIAFFLGIEPMTIQLKKYYEPQNILIY"
BASE COUNT         1743 a          818 c          954 g         1424 t
ORIGIN      
        1 cgcctcaaaa agtagtttta cactagaaaa ttggcttaag tccaaaagtc aggttttaac
       61 ataaacgaca atcttgttcg ttattagaaa ataatcatta atgataaaat acaaatatgg
      121 atatatttac acttttagaa tttaatgaaa tcgttagtaa agttaaagat ttaacaaaga
      181 cagaattagg tggtgagtac gctagtctta ttagtccttt tgagagtgaa gaagaagcaa
      241 cttacgcact taatttagta agtgaagcag agtcaatttt aacacgcttt ggtgcgttgc
      301 cactcgctcc aagtcataaa cttgccccgc ttcttgaaga agcacggcgc ggtaatattt
      361 taacaccaca cgacttttat ttaataagtg cggacgctat gaatattgtt aagattaatt
      421 attacttaaa agataagcta gataatgctc ccttattaaa agcaatgctt aaacgcgaaa
      481 aagatttagc ggtgctcgct aatgaaatta ataaagtaat tacgcctaat cttttagtaa
      541 aagataatgc gagccacgaa ttagcgcgca ttagaaaaaa cattgccttg agtgaagatc
      601 gccttaataa agcagcgcaa ggtttaatta gtaagtacgg aaacttctta gcggaaaatc
      661 ttattactaa gcgcgatggt cactttgtga ttcccgttaa aaccgcctat aaaaataagg
      721 ttgacggcat tatttatgat attagtaatt ccggccaaac aatatttgtt gaacctagtg
      781 aaatcgccgc actaaatagt actttaatta gttatcaaaa tgatgaaata attgaagtta
      841 atcgcatttt aagagcatta actttagaaa ctttaaaata tgaagacgaa attataaata
      901 ataacatctt acttgcggaa cttgatttaa tcttcgctaa agcgacttat ggtcaaagta
      961 ttaatggctt tgtccccgtt tttcaacaaa caaaagggtt acaatttaaa aaagcccggc
     1021 acccgcttat tgataagaat cttgtcgttg ctaatacttt taatttaaaa gatgataaat
     1081 tcatcattgt tataagtggg cctaacgctg gtggtaaaac agtagcgctt aaaacaatgg
     1141 gtcttttagt gctgatggca aaaagcgggc tcatgttacc cgtaagcgaa gaacctaaat
     1201 tattttttta taagaatgtg tacgctagta ttggcgacga acaaagttta agtcaaaatc
     1261 tttcgacatt cagtagtcat gtgaagcgta ttggggaaat aatggatgtt gcgactagtg
     1321 acgaccttgt attacttgat gaactcgcga ctggcactag tcctgaagaa ggggaagcac
     1381 ttgccttagc aatcgctact tatttattaa ataacagtat tagcagtgta attagcagtc
     1441 actatagtag acttaaagaa tttgcctact cccgcagtgg catcattaat gcttcaatgg
     1501 cgtttgatga aaagaaatta acaccgcttt atgaatatca tgaagaaata ccaggacgct
     1561 cctacggttt agttgtcgct aagcgctttg ggattaaaga cgaagttatt aaagacgcgg
     1621 agtccttttt aagtgacggc gtttatgact ttgaaagtac aattggtaaa ttacgcaatg
     1681 aaattagaac acttgaactt gaacgcactg aactagaaaa agagcgcgca gcactcctta
     1741 aaaaagaagc cgaattagat aaaaagctta agcgacaaag tgaagcagaa gaagacttta
     1801 agttaacgaa aaatgaacgc ttagaaaaag aaattaatga agcaattgca acacttgata
     1861 ctttagtaaa aagcgcactt gctagtggcg caaaagctca cgaaatcatt gccgtgcgtg
     1921 atgagataaa aaaacttggt aaagagagta aaaaggaagt aaaagcaccc gctaaagact
     1981 tcgaactagg cgactatgtt gaagttaaaa aactccatgt taaaggcgaa ataacgcgta
     2041 ttcgtggcga agaatatact gtaacgctcg caagcgggaa aatacttaaa gttagtgcaa
     2101 gtgaccttga aataacggag cgcactaagc gtaaaagcac accaacaatt acggttaaag
     2161 gtttagaaga agacttaact agtgtcaaac ccgaaattaa tgtgattggg atgcgtgttg
     2221 aagaagcgct taatgttatt ggaccctacc ttgaccgcgc tctacttaaa cgctatccaa
     2281 gtgtccggat tattcacggc tttggtaccg gggcactgcg gcgcggtatt catgactact
     2341 tagcgaaact ttcatttgta aaaagttaca ctctcgccgg agaatacgaa ggccaaggag
     2401 gggcgacgat cgtcaccttc taaatgaaaa ataagaagtt acaattttta attgaccatt
     2461 taccgagtga ccctggtgtg tatttaatgc tagatagtca cggtcgcatt atttatattg
     2521 gcaaggcaaa agacttaaaa agtagagtaa gtcaatattt tacgcgtccc caaccaccaa
     2581 aagtcgcggc aatggtaaag cgcaccgcta aatttgaaac aattgtgaca aacagtgaaa
     2641 aagaagcatt tattcttgag cttaaattaa ttcaaacgca ttatccgcgc tataacatta
     2701 tgcttaaaga tgattcgcat tacccatata ttgcacttac taaaaagggt gatccggtcg
     2761 ttagtataaa acgcaaaaca agtgacgata aatatattta ttttgggccg tttcccgcga
     2821 gtcaaaaggc gtatcaaatc gttgatttaa ttaataaaat ctttatgaca cggaagtgta
     2881 aaggtaaaag taaagatccg tgcttttact ttcatttagg gcagtgtccg gggtattgct
     2941 ttaaagatat aagtgactta gaaaaagata aagtacgaag tgatattctc acttttttaa
     3001 atggtaaaga taaagataca ttaaaacgct atgaaacaaa gatgaaaata gcaagtgcgg
     3061 aacttgactt tgaaaaagcg cgtgattata aagaaatagt tgacgcgata ctccatattt
     3121 atagcagtca aaatattgaa cttaaaaata aagttgattt agatgtgatt gcctttaccc
     3181 aaattaatga ttatattggg ctaaatattg tctcttatcg tgctggagta ttacttaatg
     3241 aacagtttga tgcctttccc tcttttagcg accttcatga acaaattatt aatttaattg
     3301 ggcaatacta cacaaagaat ccattgccca ctaaattaat ggtcggtaat tcatttatcg
     3361 ctgaagcgtt aacgctttat tacgataaag aagttattgt ccctagtcgc ggacaaaatc
     3421 tagaaatcct taatcgggct tatcaaaatg ccacaagtgc attgcagaag catttactat
     3481 atcatcaagc tttaggcact gaaaatgaaa gtttaacggc gctaagtgaa acgcttggta
     3541 ttaagtatcc tagtcatatt gattttgttg atattgctca tctttttggt agtaatgccc
     3601 aaggagtcgt tattacttat attaacggtc gaccatttaa gaagttatat cgtaaatata
     3661 atattgaaaa tgaaaattct tttgatgatt acgctagtat taaagaagta ctcacacgcc
     3721 actataaacg aaaactcatt gaaggaaaac atttacctga tttactaatc gttgatggcg
     3781 gtaaaggtca acttggtgtt gcgcttagtg tgaaagacga attaaatctt gatttcaaca
     3841 ttattagcct tgtgaaagat gataaacata caacaaaagc acttattaat gaagaaggtg
     3901 acgaaataag tgttaataaa aatttactca cttttctcgc cgcgattcaa aatgaagggc
     3961 accgttacgc gattactagt cacagagcta aaagagggaa agggacatac acaagcgctt
     4021 tagacgggat taaaggttta ggcaagaaac ggaagatgct cttactaaat acttataaaa
     4081 gtattgatgt cttaaaaagt gtcccactaa cagagctaag tcaacttatc ccgcgcgaag
     4141 tagcggaagc cttacttact aaattaaata gggttgatga cacggaagga ggagtttaat
     4201 atgactaaag taaagactaa aaaaggacca aaaataattc ttgccgttgc ctcactctta
     4261 gttgttacta ttctttcttt aagcattgta cgcattgtta aaaataatcc gcccaagcat
     4321 aaagattatg ccatttatct ctatggcgaa gaacatgggg tcgctaaaat caataaaaaa
     4381 gaactagcgc tctgggatga cttttatcat aagcaaggta tgcgtcatct tttcatcgaa
     4441 tcagcctatt ttgatgcagc aattcttaat ttatggatgc aagaagaaga cgacacccgc
     4501 tttgaatatc tttatgacgg ctgggaagga acattatcgc ataataaaga gacccgtgac
     4561 ttttattttg cctttaaaga aagttatcca gaaacaatat tccacggcac tgatgttggc
     4621 catcaacatg aaagagcagg acagtattac ttagattatc ttattgataa cggtttagaa
     4681 aacaccgaag catacacttt aacacttgaa tcaatcgctc aaggcgaaga atttactaaa
     4741 actggcgact taaaatatcg tgaaagtaag atggtaagta actttattcg tgaatttgat
     4801 gctttagata atgaagtgat aatggggata tatggtagtg cacatattag taaagacttt
     4861 attgctttct ttttaggcat tgaaccaatg acaattcaac ttaaaaagta ttacgaacct
     4921 caaaatatat taatatacg
//
LOCUS       DAOSTA010000056         4922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_15575_length_4922_cov_8.831518, whole genome shotgun sequence.
ACCESSION   DAOSTA010000056 DAOSTA010000000
VERSION     DAOSTA010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15575_length_4922_cov_8.831518"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..321
                     /locus_tag="PLU08_04635"
     CDS             157..321
                     /locus_tag="PLU08_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71009.1"
                     /translation="MTNTNIKLITEKTKHELFILKIDVILVRHGLKRTQIVYDRAGQV
                     AVSFVPPKRK"
     gene            384..1679
                     /locus_tag="PLU08_04640"
     CDS             384..1679
                     /locus_tag="PLU08_04640"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HOQ71010.1"
                     /translation="MNTFKTKWKEGITKIDSFFNMLFDYFMKFFRKYYLWIAFAFILA
                     ISFFVRYAFFDYLSGDMHNFLLQWFNHLKANGGLKAMGDYPWTNTLGVRAGDYPVSYI
                     TLLALLSYLPFEGIVSIKLLSVFFDYVLAIGAVLIIREFNKNSYFSLLAFAILVFFPT
                     SILNSAVWGQCDQLYVALIVWTVLFLIKKKHFLAMIFLGLALATKLQTTFFLPVLIFM
                     WLAKKFKLRYLLVMFLVIFLTFIPSYIAGAPFSMPFNMYRSQIGLYTNANYGAGSLYA
                     FFEFPKLYAGIKRVGVYVAFIAIGVTLLVLFHYKVPATPKNIIYVTALFSLVSPFFLP
                     HMHERYFYMADAFLIIYVLVFKRKYIYAALMSFSSVLTYTHFLTGQYIFKFLDKDCVR
                     LSALINLGLIIALIIDAKSVLEKEPAEELLELETEQSEV"
     gene            complement(1676..4426)
                     /locus_tag="PLU08_04645"
     CDS             complement(1676..4426)
                     /locus_tag="PLU08_04645"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOQ71011.1"
                     /translation="MKSLINKIKCIKKEDISRITFTALPFVLAFFIPFILAIILFSKH
                     GYYPFSADGHSLAMVDLRGQYIAFFRYYKTILDGDNTILYTLGKVTGGDFLSIFTYYL
                     ASPFNLVLKFVANVELPKALMWLVIIKIALSGLTAYISLAAINKNGFVNLIFSIAYAL
                     MAYNFVYYSNIMWLDGVLALPLVALGIHLLTQEKSALLYIFALAYVIATSWYIGIMVA
                     MFSVFFFLYNFISLNSRMYFRKKLLLTFTLSSLLAGLISFAFWGSALVNILGTKGSMS
                     FTNLSKRIKEFYALVDIERGFLYGNHIGMKDIMEPTSVIYAGALPLFLMIMFFANRAF
                     SRKQRIAALTLVLIYVVALFNKGVDHLFHGGPAPNWFPCRYGFIFSFIVIYFASLNFR
                     EYKATKWYAFIAPVIVYLFLIISLTKNEFAYDKKSILYFSITFCVLVIFFVLNEFIFK
                     QKGEEDNSKRVQKYVTASVSFLLIIVSAINVFGNSNRILSVFSDTVYHESMTAYRNDE
                     RIGRAIDFLKKSDNSLYRLEKSFIRSGTYNKANNDSMYFNYNGISHYSSSEKKKVMDY
                     MLKIGFHYNGFNLNYANGSTLAINSYLGVKYLLDNGVNRNFDFVRELPLVDDGLNDDI
                     NTYLNTYALPFLMPIEDTNISYVGDGVYVDGGSSVYWFDMFEYQNHLFKTITRTVKDE
                     LGKPKDIFKKATYTKTLSNVTPLPRDYYYKVVSSGTIDYDITLDKNTNYYYYIHGPKS
                     NDLALYDRNIYTTYFSYHGYQINGIRRNTASTRLSVAITKPQESIKLQEAIYYEDLSV
                     LNEYINAIKAIAEVDIKQEKTSKYSASVVTKKDNQYMLLTLPYNQNLRVFIDGKKVET
                     TTRFNIFTGFTIPKAGAHKITIKYVELTYQLGIPVGILSAAGTIVISVLNKKKKEEQE
                     KA"
     gene            complement(4401..4829)
                     /locus_tag="PLU08_04650"
     CDS             complement(4401..4829)
                     /locus_tag="PLU08_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71012.1"
                     /translation="MKFKKKVIIKLDTINILMVLSFFLPLATLRKRLPERGLVINYVS
                     GFNMFASPLWPFILPYFIPLLIFIATSFFYFKSKKVRVINFIMLLIMIILGAIAISIF
                     YGYGTLYNIAFLICVVLMITLLVNFKKEEVIQSEITDKQN"
BASE COUNT         1683 a          827 c          766 g         1646 t
ORIGIN      
        1 caaaaattag tcctacttcg tagggctttt tttatatttc atcatagtca tttttaaagt
       61 tatgagatac aataaaacat atacagtggt tagtaacatt aacactaata ttaatgcccc
      121 tcattaataa ccgcgacacg gaaaggggtt ttagctatga ctaacactaa cattaaactt
      181 atcaccgaaa aaacaaaaca cgaattattt attttaaaaa ttgatgtcat acttgtaaga
      241 cacggtttaa aaagaacgca aatagtttac gaccgagcag gtcaagtagc ggtgagcttc
      301 gtgccgccta aaagaaaata gtattccacg caataagtaa tattttaata tatggtaaac
      361 tttacttaag taagaggatg caaatgaata cttttaaaac taaatggaaa gaaggtatta
      421 cgaaaattga ctcatttttt aatatgcttt ttgattattt tatgaagttc ttccgcaaat
      481 attacttatg gattgctttt gcctttattt tagctatttc ctttttcgtc cgttatgcat
      541 tttttgatta tttaagtggc gatatgcata actttctctt acagtggttt aatcacttaa
      601 aggctaatgg ggggttaaag gcaatgggtg attacccgtg gactaacaca cttggtgtta
      661 gagcagggga ctatcctgta tcttacataa cactgctcgc ccttctttcg tatttaccat
      721 ttgaagggat tgtttcaatt aaattactta gtgtcttttt tgattatgta ctcgctattg
      781 gagcggtact tataattcgt gaatttaata aaaactctta tttctcgcta ttagcgtttg
      841 ccattttagt atttttcccc acgagcattc ttaattccgc tgtgtggggt caatgtgatc
      901 aactctatgt tgcgcttatc gtctggacag tattattcct tattaaaaag aaacactttt
      961 tagcaatgat ttttttagga ctagcgttag cgacaaaact acaaacaaca ttctttttgc
     1021 cggtgttaat ctttatgtgg ctcgctaaaa agtttaaact ccgttatctt ttagtaatgt
     1081 tcctcgttat ttttcttacc tttatcccta gctatattgc cggcgctcct tttagtatgc
     1141 cttttaatat gtatcgttca caaattgggt tatatacgaa cgcaaattat ggagcaggat
     1201 cattatatgc tttctttgaa tttccaaaac tttacgccgg aataaagcgt gttggtgttt
     1261 atgttgcttt tatcgcaatt ggcgttacac ttttagtgct cttccactat aaagtaccag
     1321 caacgcctaa aaatataata tatgtcactg cactattttc attagtgtca ccctttttct
     1381 taccacatat gcatgaacgc tatttctata tggcggacgc ttttttaatt atttatgttt
     1441 tagtatttaa acgtaagtac atttatgcgg cactgatgtc tttttcaagc gtcttaactt
     1501 atacacactt tttaactggt caatacatct tcaagttttt agataaagac tgtgtaagac
     1561 tttctgcgct aataaattta ggattaatta ttgctttaat aattgacgct aaaagtgtat
     1621 tagaaaaaga accggcagaa gaattacttg aacttgaaac agaacaaagt gaagtttagg
     1681 ctttttcttg ttcttctttt ttctttttat ttaaaacact gattacgata gtgcccgctg
     1741 cacttaatat accaacagga ataccaagtt gataagttaa ttcaacatat tttatagtga
     1801 ttttatgggc accggcttta gggatagtaa agcctgtaaa aatattaaaa cgcgttgttg
     1861 tctcaacttt tttaccgtca ataaacacgc gtaagttttg attataaggc aaagttaata
     1921 gcatatattg attatctttt ttcgtcacta cactagcgct gtacttagat gttttttctt
     1981 gcttaatatc aacttcagca atagccttaa tcgcattaat gtattcattt aaaacactta
     2041 agtcttcgta atatatcgct tcctgtaatt taatactttc ttgtggttta gtaattgcca
     2101 cacttaatcg tgtactagcg gtgttacgcc taataccgtt aatttgataa ccgtgataag
     2161 aaaaatatgt cgtgtatata ttgcgatcat ataacgctaa gtcattactc tttggtccgt
     2221 gaatatagta ataatagttt gtgtttttat caagtgtaat atcataatca atcgtgccgg
     2281 aactaactac tttatagtaa taatctcttg ggagcggagt aacattactt agcgtctttg
     2341 tataagttgc ttttttaaag atgtctttag gtttacctag ttcatctttc acagtgcgtg
     2401 taattgtttt aaataaatga ttttgatatt caaacatgtc aaaccaataa acactgctac
     2461 caccatcaac atatacacca tctccaacat aactaatatt cgtatcttca atcggcatta
     2521 aaaatggtaa ggcgtaagtg ttaagatatg tatttatatc atcattaagt ccgtcatcca
     2581 caagcggtaa ttcacgcaca aagtcaaagt tacgattcac tccattatca agtagatact
     2641 ttacacctaa atagctatta atcgctaaag ttgaaccgtt cgcataattt aaattaaagc
     2701 cgttataatg aaatccaatc tttagcatat aatccattac tttctttttt tcgcttgaag
     2761 aatagtgtga aataccgttg taattaaaat acattgagtc attattagcc ttattatatg
     2821 taccgctgcg gatgaatgat ttctctaaac gatagagtga attatcgctt tttttaagga
     2881 aatcaattgc tctgccaatg cgttcatcat tacgatatgc cgtcattgat tcgtggtata
     2941 ctgtatcact aaacactgac aaaatgcggt tagaattgcc aaatacatta atcgcgctca
     3001 cgattataag caggaaagaa acactcgccg taacatattt ttgtactctt ttagaattgt
     3061 cttcttcccc tttttgctta aagataaatt cattgagcac gaagaaaata actaagacgc
     3121 aaaaggttat actaaaatat aaaatacttt ttttatcata cgcaaattca tttttggtaa
     3181 gcgaaataat taagaataaa taaacgatta ccggagcaat aaaagcatac cattttgtcg
     3241 ctttatactc tctaaagtta agggaagcaa agtaaataac aataaaacta aaaataaacc
     3301 cgtagcggca cggaaaccag ttcggggctg gaccaccgtg gaagagatga tcaacacctt
     3361 tattaaataa cgctactaca taaattaaca ctagagttaa agctgcaatt ctttgtttac
     3421 gactaaatgc acgattagca aagaacataa tcattaaaaa tagtggtaat gcaccagcgt
     3481 aaattacact tgtgggttcc ataatatctt tcattccaat atgattcccg tataggaaac
     3541 cgcgttcaat atcaacaagc gcataaaatt ctttaattct ttttgataag ttagtaaaac
     3601 tcattgagcc ttttgtccct aaaatattta caagcgctga accccaaaag gcaaacgaaa
     3661 ttaaacctgc tagtagtgaa cttaaagtaa aggttaaaag taattttttc cgaaaataca
     3721 ttcgtgaatt tagacttata aaattgtaaa ggaaaaagaa aacagaaaac attgccacca
     3781 taatcccgat ataccacgaa gtagcaatga cataggctaa cgcaaaaatg tataaaagcg
     3841 cactcttctc ttgtgtaagt agatgaatac ctaaagcaac aagcggtaaa gctaatactc
     3901 catcaagcca cataatgttt gaataataga caaagttata cgccattagt gcatacgcaa
     3961 tactaaagat taagttaaca aaaccgtttt tattaatagc cgctaagctt atataagcag
     4021 ttaaaccact tagtgctatt ttaataatga caagccacat taaggctttt ggtaattcaa
     4081 catttgcaac aaactttagc actaaattaa agggggatgc taagtagtaa gtgaaaattg
     4141 aaagaaaatc accaccagtt actttcccta atgtatataa aatagtattg tcgccatcta
     4201 atatcgtttt ataataacgg aaaaaggcaa tatactgtcc gcgcaaatca accatcgcta
     4261 aagaatgccc gtcggcacta aaagggtaat atccatgttt tgaaaataaa ataatagcaa
     4321 ggataaacgg aataaaaaaa gctaagacaa aaggtaaagc agtaaatgtt atacgactga
     4381 tatcttcttt tttaatacac ttaattttgt ttatcagtga tttcactttg gattacttct
     4441 tctttcttaa agtttacaag cagcgtaatc attaacacaa cacaaattaa aaaggcaata
     4501 ttatagagtg tcccatatcc gtagaatata ctaatcgcaa tagcgcctag tataatcata
     4561 atgagcaaca ttataaaatt aatgacccgt acttttttac ttttaaagta gaaaaatgat
     4621 gtagcaataa aaataagaag cgggatgaaa taaggtaaga taaacggcca taatggtgaa
     4681 gcgaacatat taaagccgct gacataatta atgacgagcc cgcgttcagg taaacgtttt
     4741 cgtaatgttg caagcggtaa aaagaaactt aaaaccatta atatattaat agtatcgagt
     4801 ttaataatta ctttcttctt aaatttcatg tttcacctct ttgtttaatt atagtatgaa
     4861 aataataaat tgttacatga aaaagataaa gtgacaaaat agaaaataaa gttttaaaaa
     4921 ta
//
LOCUS       DAOSTA010000057         4857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_15872_length_4857_cov_11.256768, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000057 DAOSTA010000000
VERSION     DAOSTA010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15872_length_4857_cov_11.256768"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1204
                     /locus_tag="PLU08_04655"
     CDS             167..1204
                     /locus_tag="PLU08_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOQ71013.1"
                     /translation="MGIRFSVKSKIREWLSFRPRLKVATEWMYLLMLVLFSSFTYALA
                     FRLFISPSLETLHSVSGNQIVFVGGGVSGLSQNLVKLIHDIIGWQKVNPRVLQSILYF
                     TLNIPVFILGWLKIGRRFTIFSIINVLLASLFISIIPVSLEKAVIYDSQLTRTLYAGI
                     LAGFSAAVAFSGNVSSGGMDIIAYYFANKKSEGVGKYNVLFNVVVVGFYFVLNLIHPS
                     TNIDIANTQFTNTNVAITSFISSVTYLVMNSFVIDLINVRNKKSQVQIITNDEELAKI
                     LISNFPHGATTIKGIGVYSQSEKSVIYMTVSSNEVNDVVRLTQQVDPKAFINVYDLRQ
                     VFGKFYISPIK"
     gene            complement(1270..4212)
                     /locus_tag="PLU08_04660"
     CDS             complement(1270..4212)
                     /locus_tag="PLU08_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009273554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ71014.1"
                     /translation="MLELVNIKRYYTQKKGPTVKALDDVSLVFPETGMVFVLGKSGSG
                     KSTLLNVIGGLDQATSGDLIIKGKSTKKFKQNEFDSYRNTMVGFIFQEYNVLNDFTVG
                     QNIGIALELQGEKASNEKINAMLELLDMQGYGHRKPNTLSGGQKQRVAIARALVKNPQ
                     IIMADEPTGALDSVTGKQLFETLKKLSQDKLVIVVTHDHEFAENYGDRIIEFSDGKVI
                     RDVSKVKNEKQGTNTVFSEEGITVSAGYELTAEDLVKINEYLRQNRDKTFIKVLNNDY
                     EFKPTKQPASFPKTEKLDLIKSKMPFKSALKIGASALKHKRFRLALSIILATSAFAMF
                     GLTDAIANFKRTETTINTMLDLNIGYSSISKIERIKYSDYYYSQQVGLDDDDLAELEK
                     IDNRLDFFPVLNEALEYSSNFYSDSFDYYDPYRYGINTIAHLKDADLTKANLSLLAGT
                     LPTNKNEVAVPEYVFDSFKKYGYRSLTNSTKIAIDDPEDLLNKTLNNKFVISGVYKTG
                     LEVDRFIIENVDDDGSIYNTINTEVFRQYLLNGFHTTLLVHEEYYNKLVEQNKVYSLP
                     FNSNSYFQMGDVDRYQFTTRNFRRIESLKTNNDVILFNGITHDNLGRDDIILSTSAAF
                     FQGMEGGVHDSVQTALLVFVDNIKSTDENDLAIEQMLKTNYQHYGFNSPLDVTAPSLW
                     SEQEKTSFRNFYAQALLTFLYDENPFQPELIGREAIFAVTSAQIKTDFAPFNIEYFLV
                     AYESDGEKLKAAQDLRVVGFTFDYQYYIEAWESIIIYINEENAEVMNLTPEGAYFRAV
                     TYHGNSRAIIQKLVKFNELGTKKDVGSFYRMNNEVSYLVENVGDFLEIISRIFVVVGI
                     IFAVFASLLFLNFITLSVAFKKQEIGILRAIGARGIDVATIFMNEAGIIALINLILSF
                     MLAIIAGTTINNYIGKNINIYLSIVNVGLRQLLLLILIAFGVAFISSFIPVFRLSRKK
                     PIDAIRMR"
     gene            4326..>4857
                     /locus_tag="PLU08_04665"
     CDS             4326..>4857
                     /locus_tag="PLU08_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOQ71015.1"
                     /translation="MRNKQLRILLAVTLTLNIILAVIKLGAGYLYNSPSLVGDGFNSF
                     FDIFISVMLLIVMKTASRAADENHHYGHQKYEGLIFFGLGLFILFTTVVLGINTVKDF
                     IAYFQGAEYVLPGTPALIVAIVSVFIKVFMFIINYRAYLKLKRPALKGDAINHLSDIF
                     ATAVSIISIVLAKFNFY"
BASE COUNT         1507 a          803 c          850 g         1697 t
ORIGIN      
        1 tcaacatata ttttcaaaca aaagcaataa taaaaaaagc aatttactaa agtttctgaa
       61 atctaatagc aaaacaatgt gttttttcat taaaacaata gttttaaata gttatatttt
      121 gcaagtttat taaattaaac ctataattca agatagtgag gtaattatgg gtattcgttt
      181 tagtgtcaaa agtaaaatta gagagtggct ttcattccga ccacgcttaa aggttgcaac
      241 cgagtggatg tatcttttaa tgttagtgct ctttagttcg tttacttatg ctttagcatt
      301 tcgtttattt attagtccat cgcttgaaac gctccacagt gtcagtggta atcaaattgt
      361 gtttgtgggg ggcggtgttt ctggtttaag ccaaaattta gttaaattaa ttcatgatat
      421 tatcggttgg caaaaagtta atcctcgcgt tttacaatca attttgtact ttacgttaaa
      481 tattcccgtt tttattttag ggtggcttaa aattggtcgg agatttacta ttttttcgat
      541 tataaatgtt ttacttgcat cattatttat tagtattatt ccagttagtt tggagaaggc
      601 ggtaatttac gactcgcaat taacgcgtac actttacgcg ggcatccttg ctggttttag
      661 cgctgcggtt gcttttagtg gtaatgttag tagtggcgga atggatatta ttgcctacta
      721 ctttgcgaat aagaaaagcg aaggtgttgg taaatataat gtgctcttta atgtcgtagt
      781 cgttggtttt tattttgtcc tgaacttaat tcatccgagc actaatattg atatcgctaa
      841 tacacaattt acgaacacta atgtagcgat tacaagtttt atttctagtg ttacttatct
      901 tgtaatgaat tcatttgtaa ttgacttaat taatgtgcgg aataaaaaat cgcaagtgca
      961 aattattaca aatgatgaag aactcgcgaa aattcttatt tccaacttcc cgcatggtgc
     1021 gacaacaatt aaaggtattg gtgtgtattc acaaagtgaa aaatctgtta tttatatgac
     1081 agtttcatca aatgaagtaa atgacgtagt gcgcttaaca caacaagtcg atcctaaagc
     1141 ctttattaat gtctatgact tgcgacaagt ttttggtaaa ttttacatat caccaattaa
     1201 ataaagattg tgaacattaa agaaaatgcg cttgtaagaa gcgcattttt taatgtcaca
     1261 taaatacttc tagcgcattc taattgcgtc gattggtttc ttgcgactaa ggcggaagac
     1321 aggaataaag ctagaaataa acgctacgcc aaaggcaatg agaattagaa gtaataattg
     1381 ccttaggcca acattgacaa ttgataaata aatattaata tttttcccaa tgtaattatt
     1441 aattgttgtt ccagcgatga tggcaagcat aaatgagagt atgagattaa ttaaagcaat
     1501 aattcctgct tcgttcataa agatagtagc aacatcaatc ccacgtgcac caatcgcacg
     1561 tagtatccca atttcttgct tcttaaatgc cacacttagt gtaatgaagt taaggaagag
     1621 tagtgaggca aataccgcga agataatgcc aacaacgaca aatattctag aaataatttc
     1681 taagaagtct ccaacatttt caacaagata agaaacctca ttattcatgc gataaaaaga
     1741 accaacatcc ttttttgttc ctagttcatt aaacttaaca agtttttgaa taatagcacg
     1801 tgaattgccg tgataagtaa ctgcacggaa gtaagcacct tcaggtgtta aattcataac
     1861 ttcagcattt tcttcattaa tataaataat aatactctcc cacgcttcaa tataatactg
     1921 atagtcaaaa gtaaaaccaa caacacgtaa atcctgcgct gcttttagct tttctccatc
     1981 actttcatat gcaacaagga aatattcaat gttaaatggc gcaaagtcag ttttaatttg
     2041 tgcgctagtt acagcaaaga tggcttcgcg accaatgagt tcgggttgga acgggttttc
     2101 atcatagagg aaggtgagta gtgcttgggc ataaaaattc cggaagcttg ttttttcttg
     2161 ttcactccag agagatggtg cagtaacatc aagggggcta ttaaaaccat aatgttgata
     2221 attagttttt aacatttgct caatcgcaag gtcgttttca tctgttgact ttatattatc
     2281 gacaaatact aaaagtgcgg tttgaactga atcatggaca ccgccttcca taccttggaa
     2341 gaaagcggca gaagtgctaa gtataatatc atcacgacct aaattatcgt gcgttatgcc
     2401 attaaataaa ataacatcgt tgttagtttt taaactttca attctgcgga agttacgggt
     2461 tgtaaattga taacgatcaa catctcccat ttggaaataa gaattagagt taaagggcaa
     2521 agaatatacc ttattttgtt caacaagttt attgtagtac tcttcatgca caagtagtgt
     2581 ggtatgaaac ccatttaata aatattgtct aaaaacttca gtgttaatcg tgttataaat
     2641 actaccatca tcatcaacat tctcaattat gaaacggtca acttctaaac cagttttata
     2701 tacaccagaa attacaaatt tattgttcaa tgttttatta agcaaatctt cggggtcatc
     2761 aattgcaatt ttagtgctat tagtcagtga gcgatatcca tactttttaa aggaatcaaa
     2821 aacatattcg ggaaccgcaa cttcgttttt attggtcggt aatgtgcccg ctaataagct
     2881 gagattagct tttgttaaat ctgcgtcttt taggtgtgca atagtattga tgccataacg
     2941 atatgggtca taataatcaa aactgtcgct ataaaaattg ctagagtatt caagtgcttc
     3001 attaagtaca ggaaagaagt ctagtctatt atcgattttt tctaattcgg cgagatcatc
     3061 gtcgtctaac cctacttgtt gcgaataata ataatcgcta tatttgatgc gttctatttt
     3121 ggaaatagag gagtatccaa tatttaaatc taacattgtg ttaatcgttg tttccgtacg
     3181 tttaaaatta gcaatggcgt cagttaaccc aaacattgca aatgcactag tggctaaaat
     3241 aatactaagc gcgagtctaa atcttttatg tttaagtgca ctagcgccaa ttttaagcgc
     3301 tgatttaaag ggcatctttg atttaatcaa atccaatttt tccgtttttg gaaaagatgc
     3361 tggttgtttt gtcggtttaa attcataatc attatttaat accttaatga atgttttatc
     3421 gcggttttgc cgtaaatatt cattgatttt gactaaatct tccgctgtta attcataacc
     3481 cgcactaaca gtaattcctt cttcgctaaa gaccgtattt gttccttgct tttcgttttt
     3541 tactttagaa acatcacgaa ttactttccc gtcactaaat tcaataatgc ggtcaccata
     3601 gttttcggca aattcgtggt catgagtcac aacgatgaca agtttatctt gtgataattt
     3661 ttttagtgtt tcaaaaagtt gtttaccagt gacgctatca agtgcaccgg ttggctcatc
     3721 ggccataata atttgcgggt ttttaacaag agcgcgggcg atcgctactc gttgcttttg
     3781 tccgccggaa agtgtatttg gtttgcggtg cccgtaaccc tgcatatcaa gtaattcaag
     3841 cattgcatta attttttcgt ttgatgcttt ttcaccttgt agttcaagcg caatgcctat
     3901 attttgaccg acagtgaagt catttaaaac gttgtattct tggaagatga aaccgaccat
     3961 tgtattgcgg tagctatcaa attcattttg tttaaatttt ttggtagatt tacctttaat
     4021 gattaagtcc ccacttgtcg cttggtcaag tccgccgatg acattaagta aagttgattt
     4081 tcctgaacct gatttaccta atacaaacac catccctgtt tctggaaata cgagtgaaac
     4141 atcatcaagc gccttaacag ttgggccttt cttctgcgta taatatcttt ttatattaac
     4201 aagttctagc ataataaaat tccttatcac taatttctat aataaattat agcattatat
     4261 agaaatatag tagttttatg attaatcaat ttaattagca tttgaaaagt actataatac
     4321 aaaatatgag aaacaaacaa ctacgcatcc ttttagcggt cacattgacc ttaaatatta
     4381 tccttgcggt gattaagctc ggtgcgggat atttatataa ttcaccttcg cttgttggtg
     4441 atggctttaa ttcctttttt gatattttta ttagtgtgat gctacttatt gtgatgaaga
     4501 ctgcgagtcg cgctgctgat gaaaatcacc attatggtca tcaaaaatat gaaggactta
     4561 tcttctttgg tttaggtcta tttattttat ttacgacagt agtgttagga attaataccg
     4621 ttaaagactt tattgcttac tttcaaggtg cggaatatgt tttacctggc acaccagcct
     4681 taattgtagc gattgtcagt gtctttataa aagtattcat gtttattatt aattatcgcg
     4741 cttatttaaa actaaagcgg ccggctttaa agggggacgc gattaatcat ctttctgata
     4801 tttttgcgac agcggtcagt attatttcga ttgtccttgc taaatttaat ttttata
//
LOCUS       DAOSTA010000058         4673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_16667_length_4673_cov_10.957990, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000058 DAOSTA010000000
VERSION     DAOSTA010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16667_length_4673_cov_10.957990"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1076)
                     /locus_tag="PLU08_04670"
     CDS             complement(63..1076)
                     /locus_tag="PLU08_04670"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOQ71016.1"
                     /translation="MYQIRAKRKYSNILIAPFGRRMKAALLDLLLVLVLGFFSFMAVD
                     AIYTNSKVGSTANLTFYNIKKDSGLYHSYDESRTTAFLNLDINERDNYVRYFLGLEYF
                     YTQENTGFIYEKSVYYTEDTEFNYRTMVLKEGEDDSFFDFDTPYKTISYSFKANLSAG
                     DRNEVWKNLYANALTDLMRTPAYQKAEKVLKQFIVLNLSISAFSGTILPILAAPFFFG
                     HGRTLGKFLTGLAVVDKNGYKVSWWRMLIRSLLYGVVEIGLNFYGFFMPLLLTSGAVA
                     LTHKRQALHGIFSGTYVVDARTSKIFNNINDEEEYFSLSPEERELNKNYYQEKPYVPL
                     KKL"
     gene            1165..1956
                     /locus_tag="PLU08_04675"
     CDS             1165..1956
                     /locus_tag="PLU08_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71017.1"
                     /translation="MEKSLQPTDTKKRRRRKRPRRIRQNWFFRFLRNFVKLFKKRPLI
                     YDHNEDIVGPAIFVSNHSGAAGPMDLSMFFPYYFVPWGAHEMRGNYRTRWKYLYYVFY
                     QQKLHYGKFKSFFLATILAVINPFLYANMRLIPTYQDIRFTKSLRESLTYLDDGIPIL
                     VFPEDSSEGYFDTIIKFNEGVVVLADYVDKHRNLNIPIYPVYYSKRAREIEIGKPAYY
                     RDLKAKGHTRSEIADILRKHINTLYENIKARKARNAKAKERVRKG"
     gene            complement(2022..2897)
                     /locus_tag="PLU08_04680"
     CDS             complement(2022..2897)
                     /locus_tag="PLU08_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOQ71018.1"
                     /translation="MNLAAQLREVLNNWFASFIANESLAQILVTAIMIIFWVILAYLV
                     TLVIKLIIFRTRRVERKLGVKETKEKQTVRRLINSLIRFFFLFWIIIMILKELGLDLV
                     PLLAGAGVVAFAVGFGAQELIKDIIAGMFLILEHTFKIGDFVEIGGQSGTVVDVGLRR
                     LKIQNWKGEVITMNNGDIKVVMNASINPSIAIIEFKMDYPFNVKELETADFRAFLKKF
                     KDKNPQVLEMPEKIVLMDVNDGLLFRVTIKTESRQHIGIEREFRKELYKYVQAKNWPI
                     IVPVVVEEHDSSYQA"
     gene            complement(4597..4673)
                     /locus_tag="PLU08_04685"
     tRNA            complement(4597..4673)
                     /locus_tag="PLU08_04685"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4637..4639),aa:Arg,seq:tcg)
BASE COUNT         1500 a          857 c          791 g         1525 t
ORIGIN      
        1 ccagtcaaaa tgaaaatgtc ccacgcatct gggacttttt tattaattaa aagtaatgtt
       61 tattaaagtt ttttaagggg aacgtagggt ttttcttgat aataattttt attaagttca
      121 cgctcttcag gacttagtga aaaatattct tcttcgtcat taatgttatt aaatattttt
      181 gaggttcttg catcaacgac atatgtgcca ctgaaaatac cgtgcagcgc ttgtctttta
      241 tgtgtaagtg caaccgctcc gcttgtgagt aaaagcggca taaaaaagcc atagaaattt
      301 aagccgattt cgacaacgcc atatagcaaa gagcgaatta acatacgcca ccacgatact
      361 ttatagccgt ttttatcaac aacagcaagt ccagtcaaaa atttaccaag cgttcgtccg
      421 tgcccaaaaa agaaaggcgc tgcaagaata ggtaatattg tgccgctgaa ggcactgatt
      481 gataaattta aaacaatgaa ttgctttaaa actttttcgg ctttttgata agcgggagta
      541 cgcattaaat cagttaaagc gttcgcgtag agatttttcc acacctcgtt tctatcacca
      601 gctgaaagat tagctttaaa tgaataagag attgttttat atggtgtatc aaaatcaaag
      661 aatgagtcat cctctccttc ttttaatacc attgtgcgat agttaaattc ggtgtcttct
      721 gtataataca ctgatttttc atagatgaaa ccagtatttt cttgggtata gaaatattct
      781 aaacctaaga agtaacgaac ataattatca cgctcattaa tatctaaatt taaaaatgct
      841 gtcgtccgcg attcatcata actatgatat aaacctgaat cttttttaat gttataaaaa
      901 gttaaattag cggtgcttcc tactttacta ttcgtgtaaa tagcgtccac tgccataaaa
      961 ctaaaaaagc cgagcactaa cactaataag aggtctaaaa gtgcagcttt catcctgcgc
     1021 ccgaaggggg caattaatat atttgaatat ttccgtttag cgcgaatttg atacataacg
     1081 atattatagc aaatatgccg cactaagttt aagatttggt atcttccttt gctaaaagta
     1141 cgcttattcg gtaaaatgaa caatatggaa aaaagtttac aaccgactga tacaaaaaaa
     1201 agacggcgca gaaaaagacc acgaagaatt aggcaaaact ggttcttccg ttttttacgt
     1261 aattttgtga agctatttaa gaagcgtccg cttatttatg accataatga agatattgtt
     1321 ggtcctgcta tttttgttag taaccatagc ggtgcagcgg gtccaatgga tctttcaatg
     1381 tttttccctt attactttgt gccatgggga gcgcacgaga tgcgtggtaa ctatcgtaca
     1441 cgctggaaat atctttatta tgttttttat caacaaaaat tacattacgg taaatttaaa
     1501 agttttttct tagcgacaat tcttgcggta attaatcctt tcctttatgc aaatatgcgc
     1561 ctaattccta cttatcaaga cattcgtttt acgaagagtt tacgggaaag tttaacttat
     1621 ttagatgacg gcattcctat tcttgtattc cctgaagatt caagtgaagg ttattttgac
     1681 acgattatta aatttaatga aggtgtcgtc gtgcttgctg actatgtcga taaacatcgt
     1741 aatcttaaca ttccaattta tccggtttat tacagtaaaa gggcgcgtga gattgaaatt
     1801 ggtaaacccg cttactatcg tgacttaaaa gcaaagggtc atacccgctc tgaaatcgca
     1861 gatatcttac ggaagcatat taatacatta tacgaaaata ttaaagcaag aaaagcacga
     1921 aacgcgaaag caaaggaacg agtaagaaaa ggttaaaatg ctcttattat aacgacaaaa
     1981 ggcggctaga ttgccgcttt tttactttgc ttttaaatag tttaagcttg ataacttgaa
     2041 tcgtgttctt cgacaacaac tggcacaata attggccaat ttttagcttg tacatattta
     2101 taaagttctt tacggaattc gcgttcaatg ccgatgtgtt gccgtgattc tgttttgatt
     2161 gtaactctaa agagtaaacc atcatttaca tccattaaga caattttttc aggcatttca
     2221 agtacttgtg ggtttttgtc tttaaacttc tttaagaagg ctctaaagtc cgctgtttct
     2281 aattctttaa cattaaatgg ataatccatt ttaaactcaa tgatggcgat gcttgggtta
     2341 atactagcgt tcatcactac tttaatatca ccattattca tcgtaattac ttcacctttc
     2401 cagttttgaa ttttaaggcg tcgtaaacca acatctacga cagtgccgga ttgaccaccg
     2461 atttcaacga agtcaccaat tttaaaagta tgttctaaaa taaggaacat ccccgcgatt
     2521 atgtctttaa ttaattcttg ggcaccaaag ccaacggcaa aagccacaac cccagcacct
     2581 gctagaaggg gcactaaatc aagtcctaat tcttttaaga tcataataat aatccagaat
     2641 aaaaagaaga aacggattag gctattgata aggcgacgca ctgtttgttt ttcttttgtt
     2701 tctttaaccc caagtttacg ttcaacgcgg cgtgtgcgga aaataattaa tttaataaca
     2761 agcgtaacta aataagcaag gatcacccaa aagataatca taattgctgt tactaaaatt
     2821 tgcgcaagtg attcatttgc gataaaagac gcgaaccagt tatttaatac ttcacgtaat
     2881 tgtgctgcta aattcatatt gatatacctc cattaacgct tgtaatttta gcgaaatatg
     2941 ccactttttt ccaatgttat gttataaaat taaaagttta attaatagat gatttcataa
     3001 tggtcgtgat taaggcgatt aatcgtcact agtgccgctt cactgcttgc ccaatcgcta
     3061 tgaccatgca ccacaattcg cactacctgc ggattagtgt accatcccga aatataccca
     3121 agattaaaag aaacagtgtt acttgcacct gctggcgaag aagtttcata actataacca
     3181 tcattgtagt aaaggtaata tgaagaaccc atgccaagcg aattagcggt atcatttggt
     3241 actcttgcaa atgtaagata cattttttct tcaatattaa aaccttgtgg tgtatttaat
     3301 ttagggactg cttcaattga aaaacttagc gtttcataat cgcaattaat atgacaaaca
     3361 ccacttaaat tacctgcttt tataacaacc ggcgtatgtc tcttaatgtg atttcgctca
     3421 ttatataaac ttatttcatt cgtggaaaca gtctttttat attcaccaat ttgcacatca
     3481 tattcactaa tgccttcaac cttatcccac aagagtactt tcccgttttt taaggcaaaa
     3541 ccagtaatac ttggctcttc tccagagtta taaattacgc catcaatata cactagtgaa
     3601 agtgttgccg cttcactata aagaatgtcg ttatacgctg ctttaacact aacagtaaat
     3661 gcttcgccac tcatatattc agcataatct agtaaatcaa aatttgatgc tgttgttgtg
     3721 aaattgtgag tgccaataga aacttcaaag ctattatata agccattagc gattattgtg
     3781 actatattat catcacgaac taaagtaggt gtttctagcg ttaaattatc atcatacaag
     3841 taatatttaa gatcatgact tattacttta acagtaaatg atggcccttc ttttaaaagg
     3901 acacggcctt taaatgtaaa ttcatgaata gcgttcccac ttatgtaatc aggaagacta
     3961 atataagttt gcggaataac gcgcggatca tcactgccaa cttttatttc ggtaaaattg
     4021 gcgtttgtct cccacgaaag ttcgtaatca gtcactttaa aattcgtgta tgttatttca
     4081 aatggggatg aataaaattc ataatataca ccatcgatac aaattattgt aaaggcgtca
     4141 cttgggacac tgatagcgtg actattatgc gcggtaatcg ttaccacagt agcgcaagta
     4201 tacacttttg atagagtcat taaatctaat tcattggtat cgtatactgt ttttcgttcg
     4261 tattccgcac cagtagaacc aaccatcaca ttgtaatgag ttgcttcagt tacagcgtcc
     4321 cactttaaat aattattttc aatgcaaatg ttttttaccg tatcaagaat gcgaaatgcc
     4381 gttgatgaag aagattcaga agatgatgaa gtaatttcac tagttttttc actactagaa
     4441 gagactaatt tttcactatg ttcactgctt gaagaagagc gacatgcaga aagtaatgtc
     4501 gctacgagta atagtgatat tttaaatatt tttttcatta tgtttacccc ttctttaaat
     4561 ttatatacct tgcttttata agcattatat ttagcgtggc gtcccgggag cgactcgaac
     4621 gcccgactta cagtttcgaa gactgttgct ctatccaact gaactaccgg gac
//
LOCUS       DAOSTA010000059         4543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_17282_length_4543_cov_12.595365, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000059 DAOSTA010000000
VERSION     DAOSTA010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17282_length_4543_cov_12.595365"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /gene="raiA"
                     /locus_tag="PLU08_04690"
     CDS             <1..542
                     /gene="raiA"
                     /locus_tag="PLU08_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOQ71019.1"
                     /translation="KNIVVTDAIRNALEKKLSRMNKYFVIDNDIDCRAVVSKIRSTAK
                     VEVTIFTKHMIFRAEESDADLYAAFDKAIDSLEGQMRKLKTRLSRRNRVGLGEAIAQA
                     IAFDNIEDATVNEAENKVVRLKSIYLEPMSLEDAIMRMEALSHKFFLYLDAEDNLISL
                     LYIRNDGGYGIIQAENKVK"
     gene            641..1477
                     /locus_tag="PLU08_04695"
     CDS             641..1477
                     /locus_tag="PLU08_04695"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOQ71020.1"
                     /translation="MKKNYKLLATDLDGTLFYPKRPRSLISRKNKKFLKKFMDEGNKV
                     VLVTGRSPAYTKNLFEVLGQEVDVIGMNGAYTIVDGQIRDEHFLDFPIEKMLYDLNAL
                     FPILGTMLISQKYPLLISTPPIGKFIQFFYNVYYFLQGRYAEDYHFSNEEFIAEIESK
                     HVYKLMIFFGMSRSGVKRAARANQYLREHYPHQFEASWTGGFIEITPVGVSKANGIKK
                     YAAEKNIPEENINVVGDSGNDISMFKAFYEHSFCMKHSHKNVKKYAKTIIRRFHHLEK
                     YI"
     gene            1491..2348
                     /locus_tag="PLU08_04700"
     CDS             1491..2348
                     /locus_tag="PLU08_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71021.1"
                     /translation="MNQVTQYILGIYQINMIIRDTVSYVAPRKEQSFSKEIYEHRGRS
                     LELLTAEGSPFAHFVSINKEKADKLVENINEFKKEMYSPESRVFRVVGEGIEVDDKMH
                     HRVYEMAIGIYQTLLDVLGGYIKYAKDNDQLELRIEELVAADEYFFRSLSYFALINDI
                     FKLFKEFSDAMQQHKGEPNPIAKFINEDINKMVQLIAFMNKHNRVTNLTYKKMTDLTN
                     AFIEHMGGQRQLPEGKNFPELFTELNEFALKTLQDAETNWRTIFLPVAKAHQEEMKKN
                     ERKNPEDLS"
     gene            2351..3211
                     /locus_tag="PLU08_04705"
     CDS             2351..3211
                     /locus_tag="PLU08_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOQ71022.1"
                     /translation="MRKTALIFDSTAIIDEEIVKKYDITFVSMNVAIDGVNHRALDID
                     DEEFRKQFHNLKNLKSGSPSILDFEEAINAKFDEGYKEVIVLPLASGLSATFNIAVMA
                     REGLSPERKAATYIHESNNASIGMDLLMHSLVELLDTDPTADELLAALEARAPQNRVL
                     FEIDNLRHLFRGGRLNPLKYIIGTALKIKPLVEFKDGELKVIAQNRSRRKTMDVILGK
                     IDDFVQNFKNVYVGLFTYDKEDNIFKAIHDIIKERWPHIRINFSKKICPIFTTHVGVD
                     GYSVAVVSYN"
     gene            3221..3547
                     /locus_tag="PLU08_04710"
     CDS             3221..3547
                     /locus_tag="PLU08_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71023.1"
                     /translation="MAKRKIDGGELALYIIYGVIALGGLTLVVLHLIGMNLANLENAL
                     RVADETFAEKMKMDFLVFGSLLVVLAGALSAITLAIYGNRAELEEEKRARRRQRMALE
                     DFSDLE"
     gene            complement(3576..4451)
                     /locus_tag="PLU08_04715"
     CDS             complement(3576..4451)
                     /locus_tag="PLU08_04715"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HOQ71024.1"
                     /translation="MHKDKIINAFKIYLNKDEVNLEYQERFLGGMSNYTYHVKVNGKD
                     YVIRIANKEGKVFVDYATEKLHLFLLEPFNITSKTLFYDTDTGIKISEYIPGDNLTAT
                     LSAEDYAGVARALHKLHALNIEGVDYGQIDRLARYESLVEKHLTPEYFELKAFWMNEN
                     STTYKDADIVFTHGDAQRTNIVKNGDSYTLVDFEFAGMNDRYFDIASFGNINFSDSLN
                     LLDYYLGRKALPHEVRRVKFHRLFQVLQWHVVALQKHLNGQSEKLLIDFKKYSDNYLA
                     FAAYLATKIKALPIK"
BASE COUNT         1539 a          741 c          855 g         1408 t
ORIGIN      
        1 gcaaaaacat cgtcgtcacc gacgcaataa gaaatgcgtt agaaaagaaa ctttcacgca
       61 tgaacaaata ctttgtcatc gacaatgaca ttgactgtcg cgcagtagtg tcgaaaattc
      121 gctcgacagc gaaggttgaa gtaacaatct tcactaagca tatgatcttc cgcgcagaag
      181 aaagcgatgc agatctctat gccgcctttg ataaggcaat tgattcatta gaagggcaaa
      241 tgcggaaact taaaacgagg cttagtcgac gcaatcgcgt tggtttaggg gaagcaattg
      301 ctcaagctat tgcctttgat aatatcgaag acgcaactgt taatgaagct gaaaacaaag
      361 tcgtacggct taaatcaatc tatttagaac caatgtcact tgaagatgcg attatgcgca
      421 tggaagcctt gtcgcataaa ttcttccttt atcttgatgc tgaagataat ttaatctcat
      481 tactttatat ccgtaatgat gggggatatg gtattatcca agcagaaaat aaagtaaaat
      541 agttaatagc gacgaagttt atcaaaaaaa tcggctagga tatcctagcc ttttctttat
      601 ttcaatagaa ataaaaaata tattgtataa ttattacgct atgaagaaaa attacaaatt
      661 attagcaact gatttagatg gaacactctt ctacccaaag cgaccacgtt cgcttatttc
      721 acgaaagaat aaaaagttct taaagaagtt tatggatgaa ggcaataaag tcgtattagt
      781 caccggaaga agtccggctt atacgaaaaa tctcttcgaa gtgcttggcc aagaagtcga
      841 tgtgattggg atgaatgggg cctacactat tgttgatggc caaattcgtg atgaacattt
      901 tttagatttt ccaattgaaa aaatgctcta tgatcttaat gcgctctttc ccattcttgg
      961 tacaatgcta atctcacaaa aatatccgct tttaatctcg actccaccga tcggcaagtt
     1021 tattcaattc ttttacaatg tttattactt tttacaaggt cgctatgccg aagattatca
     1081 tttttctaat gaagaattta ttgctgaaat tgaatcaaaa catgtctaca aattaatgat
     1141 cttttttgga atgtcgcgga gtggtgttaa aagagctgcg cgcgctaatc aatacttgcg
     1201 tgaacattat ccgcatcaat ttgaagcaag ttggacaggc ggatttatcg agattacgcc
     1261 ggtaggagtt tcaaaagcta acgggattaa aaaatatgca gcggagaaaa atattcctga
     1321 agaaaatatt aatgttgttg gtgattcggg gaatgatatt tcaatgttta aagcatttta
     1381 tgagcattct ttctgtatga aacactcaca taagaatgtt aaaaaatatg ccaaaacaat
     1441 tattcgacgg ttccatcatt tagaaaaata tatttagaaa gaaggtattt atgaatcaag
     1501 ttacacaata tattttgggt atttaccaaa ttaatatgat tattcgtgat acggtaagtt
     1561 atgttgcgcc acgcaaggaa caatctttta gtaaagaaat ttatgaacac cgcggacgta
     1621 gtctagaact tttaacagca gaaggttccc cttttgcgca ctttgtatcc attaataaag
     1681 aaaaagctga caaactcgtg gaaaacatta atgaatttaa aaaagaaatg tattcaccag
     1741 agtcacgagt ctttagagta gtaggagagg gtattgaggt tgatgataaa atgcatcacc
     1801 gggtttacga aatggcgatt gggatttatc aaacactgct tgatgtttta ggcggataca
     1861 ttaaatacgc taaagataat gatcaattag aattacgaat tgaagaacta gtggccgctg
     1921 atgaatattt ctttagaagt ttatcgtact ttgcccttat taacgatatc tttaaattat
     1981 ttaaagagtt tagtgatgca atgcaacaac ataagggtga accaaatcct attgcaaagt
     2041 ttattaacga agacattaat aaaatggtgc aacttattgc ctttatgaat aaacataatc
     2101 gtgttactaa cttaacttat aagaaaatga ccgatttaac aaatgcattt attgaacata
     2161 tgggtggtca gcggcaactt cctgaaggta aaaacttccc tgaattattt actgaattaa
     2221 acgaatttgc gcttaaaaca ttacaagatg ctgaaactaa ttggcgcaca atctttttac
     2281 cagtagcgaa agcccaccaa gaagaaatga agaaaaatga aagaaagaat ccggaggatt
     2341 tatcataaat atgagaaaaa cagcacttat ttttgattca acagcgatta ttgatgaaga
     2401 aattgttaaa aaatatgaca ttacttttgt atcgatgaat gtagcgattg atggtgtcaa
     2461 tcatcgcgca ctcgatattg atgatgagga atttcgtaaa caattccata atttaaaaaa
     2521 tttaaaaagt ggttcaccat caattttaga ttttgaagaa gcgattaacg ctaaatttga
     2581 tgaaggatat aaagaagtaa tcgtgcttcc attagcaagc ggtttaagcg ctacttttaa
     2641 tattgcagta atggcgcgtg aaggtttaag tccagaacgc aaagctgcta catatataca
     2701 cgaatcaaat aatgcttcaa ttgggatgga tctattaatg cattcacttg tggaactttt
     2761 agacacagac cctactgctg atgaactttt agcggcgctt gaagctcgcg ctccacaaaa
     2821 ccgcgtgtta tttgaaattg ataatttgcg tcatcttttc cgtggtggaa ggcttaatcc
     2881 gttaaaatac attatcggca cagcacttaa aatcaaaccg ctcgtggagt ttaaagatgg
     2941 tgaattaaaa gtcattgcgc aaaacagaag tcggcgcaaa acgatggatg taatcttagg
     3001 aaaaattgat gactttgtgc aaaattttaa aaatgtttat gttggattat ttacttatga
     3061 taaagaagac aatatattta aagcaatcca tgatataatc aaagaacgat ggccacatat
     3121 tcgaattaac tttagtaaaa aaatatgtcc catcttcaca acccatgtgg gagttgatgg
     3181 ttattcagtt gcggttgtaa gttataatta ggaggaagtt atggcaaaaa gaaagattga
     3241 tggtggagaa ttagcgcttt acattattta tggcgtgatt gcccttggtg gtttaactct
     3301 agtcgtatta catttaattg gaatgaatct tgctaatcta gaaaacgcac tgcgtgttgc
     3361 cgatgaaaca ttcgcggaaa aaatgaaaat ggattttctc gtctttggtt cattacttgt
     3421 tgtcctagcg ggcgcattaa gtgcaattac tttagcaatc tatggtaacc gcgcggaatt
     3481 agaagaagaa aaacgcgctc gtcgtcgtca acggatggcg cttgaagatt ttagcgattt
     3541 agaataatta ctaattattt atgaaaaaaa gagcactact taatcggtag tgcttttatt
     3601 ttagttgcta agtaagcggc aaatgcgagg taattatcac tatatttttt aaaatcaatt
     3661 agtaatttct cactttgccc atttaaatgt ttttgtagcg ctacaacatg ccactgtaat
     3721 acttggaaaa gacgatgaaa tttaacacgg cgcacttcat gtggtaaagc ttttcttcct
     3781 aaatagtaat caaggagatt aagactatca ctaaaattaa tattaccaaa actagcaata
     3841 tcaaaatagc gatcgttcat tccggcaaat tcaaaatcca ctagtgtata actatcaccg
     3901 tttttaacaa tgtttgtgcg ttgggcatcc ccatgcgtaa aaacgatgtc agcatcttta
     3961 taagtcgtag agttttcatt catccaaaat gctttaagtt caaaatattc tggtgttaag
     4021 tgtttttcta caagtgactc ataacgcgca agacgatcaa tttgaccgta atcaacacct
     4081 tcaatattta aggcatgcag tttatgaagg gcacgcgcta caccagcata atcttctgct
     4141 gataatgtag cggttaagtt atcgccagga atatattcag agattttaat cccggtgtct
     4201 gtgtcgtaaa atagtgtttt acttgtaata ttaaatggct ctagtaaaaa aagatgaagt
     4261 ttctcagttg cgtaatcaac aaacaccttc ccttccttat tagcaatacg gataacataa
     4321 tcttttccat tcacttttac gtgatatgta taattactca tcccaccgag gaatcgttct
     4381 tgatattcga gatttacttc gtctttatta agatatatct taaaagcatt aataatcttg
     4441 tctttatgca tggatatatc atactacttt ttaattaaat ttgttatgat taagtatgcg
     4501 cgctggtagc tcagtggata gagcaatcgc ctcctaagcg gta
//
LOCUS       DAOSTA010000060         4327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_18342_length_4327_cov_7.066011, whole genome shotgun sequence.
ACCESSION   DAOSTA010000060 DAOSTA010000000
VERSION     DAOSTA010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18342_length_4327_cov_7.066011"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..776
                     /locus_tag="PLU08_04720"
     CDS             132..776
                     /locus_tag="PLU08_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71025.1"
                     /translation="MKTLNEGESNTIKKPTSALEKALVILLVVFLGATITMLVFYHFA
                     RWGLGLNIFVFLLILIVPALLLLGVIIIIVNIAGKRNFEISWKRPLLPTFIVLGAGVF
                     SLLFVIGERATFKIRYTFTIEKWAKATPDERSVLVDSFLEQYDLNTFNDAMVIEYLGE
                     PDFKETLEVGEPPSFAGYYYIYNLGFVRDFIDPTFFDIKTDASGKVVYYNVRYT"
     gene            796..1836
                     /locus_tag="PLU08_04725"
     CDS             796..1836
                     /locus_tag="PLU08_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71026.1"
                     /translation="MKRKKQVFRASLTFIALSFLLIGSLLNNKEDYFYRLKGETTYSC
                     ALPAGYVEINTIASTALTLTSHGGGNDYHNKVNTDTFRGTVTRKAGNTAYIQRRNQSN
                     GELDSLYLWGVGTYSDLVPGAVIDISGGMIVNYYGNPEIKGFTYTVPFATNPYGYGPL
                     VYNSVSDFNINAFTNIYDNSALNYSRLVKVKNVTLNALITEGAQNRTAIRNIYNNANS
                     SGLWNFDYYIQDLAIPYLICNAEFKSGDYSFKWYKGGTDGQINFYMLVPFNPSETDTI
                     SFETSFSGAGYKYDSGNEKYANQFQFYLYKITERYDWFRIDKVTFDKTNGKFKCEVFS
                     NVDNSTTTFTEV"
     gene            1989..3227
                     /locus_tag="PLU08_04730"
     CDS             1989..3227
                     /locus_tag="PLU08_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013410846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ71027.1"
                     /translation="MQEIKRDLYLNKLINAIGNRMIKVVTGIRRSGKSYLLNNLFKNY
                     LLESGVSADHIIILNMDERRNISYRDPDTLDEYIRSKIKDNEMYYVIIDEVQFVADFE
                     SVLNGFLNMHNLDVYVSGSNSKFLSNDIITEFRGRGQQIRVYPLSFSEFMSVTTLSKS
                     DALTEYLRYGGMPLLMSMKSDEAKARYLKDLFELTYLKDIIERNNVLRDDVLDELVNI
                     LASAVGSLTSVTKLTNTFKSKGVKELSKNTLIQYLDYLLDSFLIEKVSRYDVKGKKYI
                     QTLAKYYFSDIGLRNARLNFRQQEENHIIENVVYNELIRRGFNVDVGIVEIRNGNGIK
                     RLEVDFVCNEASRRYYIQVTTHLDTREKTLQEKRPLLHIGDNFKKIIIVRDDIKPWYT
                     EEGILVLGLYDFLLDNDSIL"
     gene            3401..3664
                     /locus_tag="PLU08_04735"
     CDS             3401..3664
                     /locus_tag="PLU08_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71028.1"
                     /translation="MQTFNYIGLIVVALLLIPNLMYYLKHKEEVVSKNGIFKTLAIIG
                     ISGSLFFMVINIPYTWLQFYFMFAREMYFISNVNSLLFMYLFS"
     gene            3739..4327
                     /locus_tag="PLU08_04740"
                     /pseudo
     CDS             3739..4327
                     /locus_tag="PLU08_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
BASE COUNT         1443 a          581 c          804 g         1499 t
ORIGIN      
        1 taagtaaaat tcataaacga aagaaacaat taacttgttc attgaatttt atgttaagtt
       61 aattaaaaag tctttgttat gaaaatatta ttagcaaaag tgcaaattta ttaatttgct
      121 ataatttgat tatgaaaact ttgaatgaag gagaaagtaa cacgataaaa aagcccacta
      181 gtgcacttga aaaagcctta gttattttac ttgtggtttt tttaggcgcg actataacaa
      241 tgctcgtctt ttatcacttt gcgcgctggg ggttagggct taatattttt gtatttttat
      301 taatattaat tgtaccagcg cttttactgc ttggtgtcat cattattatc gtcaatattg
      361 ctggaaaaag aaattttgaa atatcgtgga aaagaccact attgccaaca tttattgtac
      421 ttggtgcggg agtattttca cttttattcg tgattggaga acgggctact tttaaaatcc
      481 ggtacacttt tacgattgag aagtgggcaa aagcaacacc cgatgaacgt agtgttctag
      541 ttgattcttt tcttgaacaa tacgatttaa atacatttaa tgatgcaatg gttattgaat
      601 atttaggtga gcctgacttt aaagaaacac ttgaagttgg tgaaccccct tcatttgccg
      661 gttattatta tatttataat cttggttttg tgcgtgattt tattgatccg acattttttg
      721 atattaaaac tgacgctagt ggtaaagttg tttattacaa tgttagatat acgtaaatta
      781 ggagaagtac tatttatgaa acgaaaaaag caagtgttta gagcttcctt aacttttatt
      841 gctctttctt ttttgcttat tgggtcatta cttaataata aagaagatta tttttataga
      901 cttaaaggtg aaacaactta ttcatgtgct ctccctgcgg gatatgttga aattaacact
      961 attgcgagca ctgctttaac gcttactagt cacggtggcg gtaatgatta tcataataaa
     1021 gtaaacactg atacttttcg aggtacagtt acgcgtaaag ctgggaatac ggcctacatt
     1081 cagcggcgta atcaaagtaa tggagaatta gatagtcttt acttatgggg tgtgggaact
     1141 tacagtgact tagtacccgg tgccgtcatt gatataagcg ggggcatgat tgttaattat
     1201 tacggtaatc ctgaaattaa aggttttact tatacagtgc cttttgcgac taatccctat
     1261 ggctacggac cactagtgta taacagtgtt agtgatttta acataaatgc tttcacgaat
     1321 atttatgaca attctgcact taattatagc cgcttagtaa aggtcaaaaa tgtgacgctt
     1381 aatgcgctta ttactgaagg ggcacaaaac cgcacggcga ttagaaatat ttacaataac
     1441 gctaattcat cagggttatg gaattttgat tattatatac aagatttagc gataccctat
     1501 cttatttgta acgcggaatt taaaagcgga gattacagct ttaaatggta taaaggcggc
     1561 actgatgggc aaattaattt ctatatgctc gttcctttta atccgagtga gactgataca
     1621 atttcctttg agacttcctt tagtggcgcg ggctataagt atgatagtgg caatgaaaaa
     1681 tacgcaaatc aattccaatt ttatctctat aaaattaccg agcgttacga ttggtttaga
     1741 attgataaag taacctttga taaaacaaac ggtaaattta aatgtgaagt attttcaaac
     1801 gtcgataatt cgacaacaac atttaccgaa gtttaatagt aaaacattta cttataataa
     1861 tattcgcact gaacaaaaac tacaaaaatg taagttttgt tcaatgactc ttgatattca
     1921 caaacaaata ttttaaaata gtactgaaca aaaactacaa aagcgtaagt tttgttcagt
     1981 gaggtgttat gcaagagata aaacgtgatt tatatttgaa taagttaatt aatgcaattg
     2041 gcaaccgcat gattaaagta gtgactggta ttcggcgctc aggcaaatca tatttgttaa
     2101 ataatctttt caaaaattat ttattagaaa gtggtgttag tgcagaccac atcattattt
     2161 taaatatgga tgaaaggcga aatatttctt atcgtgaccc cgatacttta gacgaataca
     2221 ttcgcagcaa aattaaagat aatgaaatgt attatgtcat cattgatgaa gtgcagtttg
     2281 ttgccgattt tgaatcagtg cttaatggat ttcttaatat gcataactta gatgtttatg
     2341 tttctggtag taattcaaaa tttctatcaa atgatataat cactgaattt aggggtagag
     2401 gacaacaaat tcgcgtgtat ccactttctt ttagtgaatt tatgagtgtc accactttaa
     2461 gtaaaagtga tgctttaact gaatatttac gctatggcgg gatgccgctt ttaatgtcaa
     2521 tgaaaagtga tgaagcaaag gcgcgttatc ttaaagattt atttgagctt acttatctca
     2581 aagacattat cgagcggaat aatgtgctgc gtgatgatgt tttggatgag cttgtaaata
     2641 tattggcctc tgctgttggt tctttaacta gtgttactaa attaacgaat acctttaaga
     2701 gtaaaggtgt caaggaacta tcgaaaaata cgcttattca gtatttagat tacctattag
     2761 attcttttct tatcgagaaa gttagtcgtt atgatgttaa aggtaaaaaa tatattcaaa
     2821 ctttagcaaa gtattatttt agtgatattg gattacgtaa tgccaggctt aatttccggc
     2881 aacaagaaga aaatcacatt attgaaaatg ttgtctataa cgaacttata agacgtggtt
     2941 ttaatgttga tgttggtatc gttgaaatta gaaatggaaa tggtattaag cgccttgaag
     3001 ttgattttgt ttgtaacgag gcgagtagac gctattacat tcaagtaacg acgcatcttg
     3061 atactagaga aaaaacattg caagaaaaaa gaccactgct acatattggc gataacttta
     3121 agaagattat tattgtccgc gatgatatta agccttggta tacagaagaa ggtattttag
     3181 tgctaggact ttatgatttc ttacttgata atgatagtat tctctaaaca cttattgtag
     3241 gctgttcttt aattggattg tgcttaatat aataaaaaat gaaaggtttt ttaattgtca
     3301 tttcttacat attctttaca aacaagcagc aataaaagta ttgaacttat gttaaaatta
     3361 tgtggaataa agacaaaggg taatgttagg agtaatagtt atgcaaacat ttaattacat
     3421 tggtttaatt gtggtagcgt tacttttaat acctaatttg atgtactatc taaaacacaa
     3481 agaagaagtt gtttctaaaa atggtatctt taaaacttta gcgataattg gtatcagtgg
     3541 atcactattt tttatggtga ttaatattcc ttatacttgg ctacaatttt attttatgtt
     3601 tgcgcgagaa atgtacttta ttagtaacgt aaactcgcta ttatttatgt atttattttc
     3661 ttagtaactt gggataaaaa gagtgtagta cgcgcgatat tattaacgat tattcctttt
     3721 tgtatattta ctttttcagc gataatgctt ggttgtttag atgaaaaaat taccgctgtt
     3781 aacccggatt caaaattatc tttatttggt caagaatata atcctgaaac ttatgcaata
     3841 gcaaaagctg acatgttaat taaaggtggt aatgcccaaa acatgaaatt tggcaatact
     3901 ttaaatgacg accaatttag tggttatgaa tttgattata ttatttcaaa tccgccattt
     3961 gggattgatt ggaaaagtga agcaaaggat gttaagaaag aacatgaaaa attaggtttc
     4021 agtggtagat ttggccccgg tttaccagca ataagtgaca gtcaaatgtt atttatgtta
     4081 aatggtgtca aaaagttaaa agaaggaagc ggaagaatgg ctatcattca aaacggctct
     4141 tcacttttta ctggtgatgc tggtagtggg ccaagcgaaa ttagaagata tattattgaa
     4201 aacgatatgt tagaagcgat tattcagtta ccaacagatt tattttataa tacttcgatt
     4261 acaacctata tatggttaat tacaaaagga aaatcaagtg aacgtattgg taaagttcac
     4321 ttgattg
//
LOCUS       DAOSTA010000061         4110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_19585_length_4110_cov_13.872503, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000061 DAOSTA010000000
VERSION     DAOSTA010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19585_length_4110_cov_13.872503"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..166
                     /gene="rpmG"
                     /locus_tag="PLU08_04745"
     CDS             17..166
                     /gene="rpmG"
                     /locus_tag="PLU08_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004028373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOQ71029.1"
                     /translation="MRKKILLICEVCLSRNYNTSVNKATQTERFQIKKYCPKCNKMTL
                     HKESR"
     gene            177..377
                     /gene="secE"
                     /locus_tag="PLU08_04750"
     CDS             177..377
                     /gene="secE"
                     /locus_tag="PLU08_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOQ71030.1"
                     /translation="MGLRKYSNEVVKEAKRVRWPKPDVLFPTIVVVLVIVFFAAFMLF
                     VEDLAVGKLLGEIERVFQGFKK"
     gene            386..940
                     /locus_tag="PLU08_04755"
     CDS             386..940
                     /locus_tag="PLU08_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOQ71031.1"
                     /translation="MAENMEMKWYVASTQSGQENTVVHNIRRRLESSDKADTVARIIV
                     AEIEEEVLNKDNKIVIKKRNLYPGYIFLEMEMSDEMWFLIRNTPGVTGFIGSSGRGAK
                     PFPVEYEELEPILKRLGHVDKTMYERYAVDDYVRVIDGPLVGTEGRIIEIDRETGNVK
                     IEAVFFGRVTTADINFALIEKVEF"
     gene            1206..1622
                     /gene="rplK"
                     /locus_tag="PLU08_04760"
     CDS             1206..1622
                     /gene="rplK"
                     /locus_tag="PLU08_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOQ71032.1"
                     /translation="MSKKIAKVVKLELIGGEAKPGPKLASVGVIMPKFCTDFNAQTGD
                     RRGEVVPVIITAYEDKSHSFILRTTPTSILLRKAAGDIKKGAGNPKVEKVGKITQTQL
                     REIAEYKLPDLNANDVEAAMKIVAGTARNMGIVVED"
     gene            1663..2346
                     /gene="rplA"
                     /locus_tag="PLU08_04765"
     CDS             1663..2346
                     /gene="rplA"
                     /locus_tag="PLU08_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOQ71033.1"
                     /translation="MKRGKKYKAAAALVDKEKLYTIEEAVALVKQTSTTKFDATIDVS
                     FRLNVDPRHADQQIRGTLVLPHGNGKEKTVLVITNSPEAAKEAGADFVGGKDMIEKIK
                     SENWFGYDVIVATPDMMGELGKLGRLLGPKGLMPNPKTGTVTTDVAKAVQEIKAGKVE
                     YRVDREANMHVSIGRVSFEDEKLIDNLNALTERIVRVRPAAVKGTFIKNAVIHTTMGP
                     SIKITFPGR"
     gene            2491..2979
                     /gene="rplJ"
                     /locus_tag="PLU08_04770"
     CDS             2491..2979
                     /gene="rplJ"
                     /locus_tag="PLU08_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOQ71034.1"
                     /translation="MNQKSIVAKEAIVKEVVDLAKSHEAFIVFTYHGLSVSQITELRR
                     LLRADGNTMGVYKNSLVERAVKELGREELNDYLTGPNAFLFSKDVSSGAKALTRFARF
                     NDVVEIKGGLFEGNRVVDGAEVRNIGRLPDRNGMISMFLSVLNAPIQKFAATVKAVAE
                     KQ"
     gene            3004..3378
                     /gene="rplL"
                     /locus_tag="PLU08_04775"
     CDS             3004..3378
                     /gene="rplL"
                     /locus_tag="PLU08_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOQ71035.1"
                     /translation="MAKLTVTDIIAALKEMTVLELNDLVKAVEEEFGVTAAVATAGPA
                     PEDDGPVDKGPSEVTLVLKHWGQSKVAVIKAVQAITGLGLMDAKKLVDGAPVNIKEKI
                     SPVEAEEHKKALVEAGADVEIK"
     gene            3449..4048
                     /locus_tag="PLU08_04780"
     CDS             3449..4048
                     /locus_tag="PLU08_04780"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOQ71036.1"
                     /translation="MNHYFANNNELEHDIRTLELSLQGTNLTLITDRGVFSNKEIDEG
                     SIVLLKSIIKSIDVKGEVLDVGCGYGTLGLYLAARYPEIKVTMFDINERAVSLTSENA
                     KRNNLTNTHVYVDSDYQALPKHTFNLIVINPPIHAGKKAYYPLLSAARDYLTDGGILM
                     FVIRKSHGAKSAAKYLSDYYSNITLIQKDAGFYVYHAQK"
BASE COUNT         1414 a          766 c          874 g         1056 t
ORIGIN      
        1 aaaaggagta taatatatgc ggaaaaagat tcttttaata tgcgaagttt gcttgtcacg
       61 gaattacaac acatccgtca acaaagccac gcaaacagaa cgcttccaaa ttaaaaaata
      121 ttgtccaaag tgtaataaaa tgacattaca caaggaaagt cggtaggaga aagattatgg
      181 gattaagaaa gtattcaaac gaagttgtta aagaggcaaa gcgtgtccgt tggccaaaac
      241 cagatgtgct tttcccaaca attgtcgttg tgttagttat tgtctttttt gctgctttta
      301 tgttatttgt agaagaccta gcggtcggta aattactcgg tgaaattgaa cgcgtcttcc
      361 aaggatttaa gaagtaggta acgttatggc agaaaatatg gaaatgaaat ggtacgttgc
      421 aagtacgcaa tcaggacaag aaaacacggt tgtacataac atccgtcggc ggcttgaatc
      481 aagtgataaa gctgacactg ttgcgcgcat catcgtagcc gaaattgaag aagaagtatt
      541 aaataaagac aataaaattg ttattaaaaa acggaactta tatccgggtt acattttctt
      601 agaaatggaa atgagcgatg agatgtggtt ccttattcgt aatactcctg gtgttactgg
      661 ttttattggt tcatcaggtc gtggggcgaa gcctttccct gttgaatacg aagaacttga
      721 accaatctta aaacgtttag gtcatgttga taaaacaatg tatgaacgtt acgcagtgga
      781 tgattatgtc cgtgttatcg acggaccact cgtgggcact gaaggacgga tcattgagat
      841 tgatcgcgaa acaggaaatg ttaaaattga agcagtcttc tttggtcgcg ttaccaccgc
      901 tgatatcaac tttgcgttaa ttgaaaaagt tgaattttag ataaaaacaa ctctaaaaac
      961 attcacccca gcattgtgct ggggtgtttt ttaatcaaat tatcataaaa tgatagtcat
     1021 aaaatgctgt ttttaacata aaacacgaaa aattacaatt tatcgttgcc caaaaatatt
     1081 tttttggtat aatcatatgc gtatattgct cgcgatgtgc ggacataata cgctcgtggg
     1141 aggaagaggc gatcttcccc ttaccacagc acggacatat ctaaaaaacg aaggaggaaa
     1201 gtcacgtgag taaaaaaatc gcaaaagtcg taaaactcga acttatcggt ggcgaagcta
     1261 aacctggtcc aaaattagcg tcagtaggtg ttattatgcc taaattttgt actgacttta
     1321 atgcccaaac cggtgatcgt cgcggtgaag tagttccagt aattattacg gcatatgaag
     1381 acaagtcgca ttctttcatt ttaagaacga cacccaccag cattctttta cggaaagccg
     1441 ctggggacat caaaaaagga gcaggcaatc ctaaagtcga aaaagttggg aaaattaccc
     1501 aaacccaatt acgcgaaatt gccgaataca aattacctga cctcaatgct aatgacgtcg
     1561 aagccgcaat gaaaatcgtt gctggtaccg cacgtaacat gggcattgta gtcgaagatt
     1621 agtggaagag aacccactcg ttagtaccac agaggaggaa atatgaaaag agggaaaaag
     1681 tacaaagccg cagcggcttt agtcgataaa gaaaaacttt atacgatcga agaagcagtt
     1741 gcgcttgtca aacaaacttc aacaactaaa tttgatgcca ctatcgatgt ctcatttaga
     1801 ctaaatgttg acccacgtca tgccgaccaa caaatcaggg gaacactcgt gttgccacac
     1861 ggtaatggta aggaaaaaac agttcttgtc attactaata gtccagaagc cgctaaagaa
     1921 gcaggcgctg acttcgtcgg gggtaaagac atgattgaaa aaattaagtc cgaaaactgg
     1981 tttggttatg atgttatcgt cgccacccca gatatgatgg gcgaacttgg gaaattagga
     2041 cgattactcg gtccaaaagg acttatgcct aacccaaaaa ccggaaccgt cacgactgat
     2101 gtcgctaaag ccgtccaaga aattaaggct ggtaaagtcg aatatcgtgt tgaccgggaa
     2161 gcaaatatgc acgtgtcaat tggccgcgtg agcttcgaag acgaaaaatt aattgataac
     2221 cttaacgcac ttaccgaacg cattgtgaga gttcgtcccg ctgcagttaa aggaacattt
     2281 atcaaaaatg ctgtgatcca tacaactatg ggtccatcga tcaagattac attccctggt
     2341 cgataagcac aaactacgct atcaataacc taagaaaaca acggcggaaa taagcttaat
     2401 aatgttgtcg aggaaagaat ataaattagt gaatatcctt gtgggattat tgaagcctca
     2461 attaccaaat taacggaagg aggatgaaca atgaatcaaa aaagcatcgt tgcaaaggaa
     2521 gcaattgtta aagaagtagt cgatttagct aaaagtcatg aagcatttat cgtgtttact
     2581 taccacggct taagtgtttc acaaattact gaattacgtc gcttattacg cgctgatggc
     2641 aacacgatgg gtgtatataa aaactcatta gtcgaacgcg cagttaaaga acttggccgc
     2701 gaagaattaa atgattattt aaccggacca aacgcattct tatttagtaa agatgttagt
     2761 tcaggcgcta aagcattaac acgcttcgca cgcttcaatg atgtcgtcga aattaaaggt
     2821 ggtcttttcg aaggaaaccg tgtagtcgat ggcgcagaag tacgtaacat tggacgctta
     2881 ccagatcgca acggaatgat ctcaatgttc ttatcagtgc tcaatgcacc aattcaaaag
     2941 tttgccgcta cagttaaagc ggtcgcagaa aaacaataaa attgcccaag gaggaaaaga
     3001 aaaatggcaa aattaactgt tacagacatt attgcggcat taaaagaaat gaccgtactc
     3061 gaattaaatg atttagttaa agcagtcgaa gaagaattcg gcgttaccgc agcagtcgca
     3121 actgctggac cagcaccaga agatgatggc ccagttgaca aaggtccatc agaagtcacc
     3181 ttagtgctca aacactgggg tcaaagtaaa gttgcagtca ttaaagctgt ccaagctatc
     3241 actggcttag gcttaatgga tgccaaaaaa ttagttgatg gtgcccctgt caacattaaa
     3301 gaaaaaatct cacctgttga agcagaagaa cacaaaaaag ctcttgtcga agctggtgcg
     3361 gatgttgaaa ttaaataact taaataagca agatgcgaag acccacaatt catgttgtgg
     3421 gttttcgtgc gtttaaggaa ggttgaattt gaatcattat tttgcaaata ataacgagct
     3481 agaacatgat attcgcacat tagaattatc attacaaggc acaaatttaa cgctaattac
     3541 tgatcgcgga gtcttcagca ataaagaaat tgatgaaggg tccattgtac tactcaaaag
     3601 tattattaag agcatagacg taaagggcga agtacttgat gtcgggtgtg gttacgggac
     3661 gcttggctta tatttagccg cacgctaccc cgaaattaaa gtcacaatgt tcgacattaa
     3721 tgaacgcgct gtaagtctaa cgagtgagaa cgctaaacgc aacaacctca caaatacaca
     3781 tgtttatgta gatagtgact atcaggctct acctaaacat acattcaact tgatagttat
     3841 taacccacca attcatgcgg gtaaaaaagc ttattacccc cttcttagtg cggcgcgtga
     3901 ctatctcact gatggcggaa tactgatgtt tgtgattcgt aagtcccatg gcgctaaaag
     3961 tgccgcaaag tacttaagtg actattattc aaatatcaca cttatacaaa aagatgcggg
     4021 attctacgtc tatcacgcac aaaagtaaca aaatacaagt aaagcgaggc catttatgaa
     4081 aacaattaac gataaaacaa aaagactcca
//
LOCUS       DAOSTA010000062         4062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_19887_length_4062_cov_9.414774, whole genome shotgun sequence.
ACCESSION   DAOSTA010000062 DAOSTA010000000
VERSION     DAOSTA010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19887_length_4062_cov_9.414774"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..688)
                     /locus_tag="PLU08_04785"
     CDS             complement(296..688)
                     /locus_tag="PLU08_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71037.1"
                     /translation="MLIYIQLLLDYYSKAFEGLFAFHEWPKALIDKYLIDNNVELTIP
                     NVPNIEFCLAGVGYGQVSVVIPGPDRCSEIKDVFEEQNFTVEHVVENNYYKVINLEGN
                     FEMMFNYYTEPKVTPNYPGFVNMVLYQP"
     gene            complement(937..2136)
                     /locus_tag="PLU08_04790"
     CDS             complement(937..2136)
                     /locus_tag="PLU08_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOQ71038.1"
                     /translation="MKITFMLFYYDPENPTINYLYRPLWDKLIKAGHEINIITPNPTR
                     GIPLSEQEKYKKTSVEKKGAFTIHRVKCYTYHPSRFCKFKLLRRYISVSRRLAKKLRK
                     VESDVVFVSSNPPLLYSYLVTRYCARKKIPVIYNVQDIYPDNIFKENSFAYKVFNPLQ
                     VKSLARASEVVTISETMRKTLLKKGDFNNKISVIYNFDISEVRKTPLVKKYTFFDPLQ
                     FNVVYAGNIGYVQDIDVILSAAKLLVHEPRIHIHIVGEGSQAARIAKRIHDENIVNAT
                     YYPPHSVEDSAQLYKNADVNVISILPGIIKTALPLKTASCLAAQKPIIFIGLETFYHN
                     EEWVNEVSELYPINYRNYEKVAETIEKLANAKTKPKLKYDQIELFDKEKNVNEYFNLI
                     TKYLKEE"
     gene            complement(2340..2510)
                     /locus_tag="PLU08_04795"
     CDS             complement(2340..2510)
                     /locus_tag="PLU08_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71039.1"
                     /translation="MSNDEWWTYSEEYDCAVPTAKCPAKGIREFNRNRNLITGFIMIG
                     DGSVPPEYDPYI"
     gene            complement(2695..3834)
                     /locus_tag="PLU08_04800"
     CDS             complement(2695..3834)
                     /locus_tag="PLU08_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ71040.1"
                     /translation="MIVLNLKLDNILAFKDFEVNFSYPRKLKKTLIEGEHLRTIPTFR
                     YKKLVVLIGSNATGKTSLIKLIWRISLFLHRKNPSFLDSLISENNEHGNIEIDFVNEY
                     DDVAILYRAKIRIIKNNVNNQNIVEVAMYSKKLTEGDSYENSINELDNHEYVFESWQV
                     ALQEFYDNFGWQVVLPSNEELFDKFYIHADINESEKDDYLDILNNVMKTLDPAIKNVF
                     HSKDAADAIVIEYSDERKVLIQNGNDISSIPLLSSGTKYGVAISNILFSIKNHRNGLY
                     LVDEQFSYVTADIETAILAKMTALLNSGEQLFFTTHNSNILELGFPFHSFYFMKKTII
                     DGAPIINVSCASELENRNNVSPKNMFDNDAFGTAPNVDGIYSIGK"
BASE COUNT         1365 a          705 c          545 g         1447 t
ORIGIN      
        1 gaccttctgt tcccaaaaca gacgcgctac caagctacgc tacatcccga tgcttgtata
       61 tattaccaaa ggtataaaaa tttagcaagt attaacaaac ttgagttctt gctttcttgc
      121 actaacaatc gaaaaggtta atttatctat taattgatta gcggttaaat tatctttttt
      181 tatcaaatat gcatgtttct tgaagataaa aagatattta aaagactatt actctcttta
      241 aaatttgcgt ttatgcttat taagaaaatg gattatatca tcgcttcttt tatttttatg
      301 gttggtataa aaccatgttg acaaatcctg gataattcgg tgttactttt ggttcagtat
      361 aataattaaa catcatctca aaattacctt caagattaat cactttataa tagttgttct
      421 caacaacatg ttcaacagta aaattttgtt cttcgaaaac atcttttatt tccgaacaac
      481 gatctggacc aggaataaca actgaaactt gaccgtaacc tacgcccgcg agacaaaatt
      541 caatatttgg aacattaggg atagttagct caacattatt atcaattaaa tatttatcaa
      601 taagtgcctt aggccattcg tgaaacgcaa ataagccttc aaaagctttc gaatagtaat
      661 caagcagtaa ttgaatataa attagcatcc cacctgtcgc tagtctttca ttataagtgg
      721 agtctaaaaa gttatagctt atccgtatag tctcgttagc gttaatgcaa aaagtgcggc
      781 taaaaataaa aatttggtct ttttcctaat cactctccaa aaatttctta catacaatcg
      841 cattttatat taattatgct gttcgtgcaa caaataatag aaaatgtatt aaaggatagc
      901 ttcataatgc ttaaaaaagc tttaataatc agttcactat tcttctttta agtacttagt
      961 aattaagtta aagtactcat taacattctt ttctttatca aaaagttcaa tttgatcata
     1021 ctttaatttt ggcttagttt tagcattagc gagtttttca attgtttccg caaccttttc
     1081 ataattacga taattaatgg gatagagctc acttacctca tttacccatt cttcattatg
     1141 ataaaaagtt tcaagtccaa taaaaataat cggtttctgt gccgctagac aacttgccgt
     1201 tttaagtggt aatgccgttt taattatccc tggcaatatt gatattacat ttacatccgc
     1261 atttttataa agctgggcac tatcttccac actatgtggc gggtaataag tagcgttaac
     1321 aatattttcg tcatgaattc gtttagcgat tcttgcagct tgactacctt caccaacgat
     1381 atgaatatga atgcgcggtt catgcactaa aagttttgcc gcacttaaaa tgacatcaat
     1441 atcttggaca tagccaatgt taccagcgta aacgacatta aattgcaatg gatcaaaaaa
     1501 tgtatacttc ttcactagcg gagtcttccg cacttcacta atatcaaagt tatagatgac
     1561 acttatttta ttattaaagt cgcctttctt taaaagtgtt ttacgcattg tttcactaat
     1621 agtgacgact tcactagcgc gcgctaaact tttaacttgt aaaggattaa aaactttata
     1681 cgcaaaacta ttttctttaa agatattatc aggataaata tcttgaacat tataaataac
     1741 tggtattttc tttctcgcac aataccttgt tactaaataa ctataaagca gtggcgggtt
     1801 actagaaaca aagacaacat cactttctac tttacgcaat tttttcgcta aacggcgcga
     1861 cacactgata taacgtctaa gtaatttaaa tttacaaaag cgactcgggt gataagtgta
     1921 acactttaca cgatgaatcg taaacgcccc ttttttctca acactcgttt tcttatattt
     1981 ttcttgttca cttaacggaa tcccgcgtgt cggattaggg gtgataatat taatttcatg
     2041 ccccgcttta attagcttat cccaaagcgg acgatataag taattaatcg tcggattctc
     2101 ggggtcataa taaaaaagca taaatgttat tttcatactt taaagtataa cacgacacta
     2161 ataataaaac agaaatttaa gtatgatttg ccgcattatt cactaataac acaaaattta
     2221 tgctttattt ttataatatt cgcgacgtgt ttttcggtca tagataattc tagtttcgcg
     2281 ttttatttgt tgcggagtgt aacctattga ctcacgcatt ttaacaaact tttcaatact
     2341 tatatatagg gatcatattc tggtggaaca ctaccatcac caatcataat gaacccagta
     2401 attaaattac gattacgatt aaattcacga ataccttttg ccggacattt cgcggttggc
     2461 acggcgcagt catattcttc actatatgtc caccattcat cattactcat aaatatatat
     2521 cgtgttaaat cagtcacttt tcattattcc tcgcttttac ctaatcttac actcttacaa
     2581 ataatagagt caagtgtatt cttatgcaca tgaaactaaa tttcttttca aaaatagaaa
     2641 tgagaactca atattcatat ttattgtcca gaattttgga ttgcagcatg tatttcattt
     2701 accaatagaa tagataccgt caacattcgg cgctgttcca aaagcatcat tatcgaacat
     2761 attctttggt gatacattat tgcgattttc tagttctgat gcacaagaca cattgattat
     2821 tggtgcacca tctatgattg tctttttcat aaaatagaac gaatgaaaag gaaaacctaa
     2881 ttcaagaata tttgaattgt gtgtagtaaa aaatagttgt tcaccactat taagtaaagc
     2941 agtcattttt gctaaaattg ctgtttcaat gtcagcagtc acatatgaaa attgctcatc
     3001 cactaaatag agtccgttac ggtgattttt aattgaaaaa agaatattac tgatcgccac
     3061 cccatattta gtaccgcttg aaagcagcgg aatacttgaa atgtcattac cgttttgaat
     3121 taatactttt cgctcatcac tatattcaat aactatcgca tcagcagcat ctttactatg
     3181 aaaaacattc ttaattgctg gatctaatgt tttcattaca ttgtttaata tatctaaata
     3241 atcatctttt tcactttcat tgatatcagc atgtatataa aacttatcaa ataattcttc
     3301 atttgatgga aggactactt gccaaccaaa attatcataa aactcttgta aagctacttg
     3361 ccaactttca aagacatatt catgattatc taactcatta atactatttt cgtatgaatc
     3421 accttcagtg agtttcttgc tatacattgc aacttcaaca atgttttgat tatttacatt
     3481 atttttaatt attctaattt tagcgcggta taaaatagca acatcatcat attcatttac
     3541 aaagtcaatt tcaatgttgc cgtgttcatt gttttcactt ataagactat ctaaaaaact
     3601 aggattttta cgatgcagaa ataaagaaat tctccaaatt aatttaatga gagaagtttt
     3661 ccctgttgcg tttgaaccaa ttaacactac taatttctta tatctaaaag ttggaattgt
     3721 gcgaagatgt tctccttcaa ttagcgtttt cttaagttta cgcggataac taaaattaac
     3781 ttcgaaatcc ttaaaggcta gaatattatc taacttcaaa tttaagacaa tcatattatt
     3841 tgctccttgt atgttcaagt tttttgaact tacaatttta atgtaataaa ttttaatttg
     3901 aaaaacaaat attttcaaac tttttaaaac tttttaaaaa aatgttaaat acttaaatag
     3961 aatatgcagc gaccaataaa ttaaaatcaa tttttaaaaa aagactacat tttgatgtag
     4021 ccttattttt agtaaatagt tttatgacaa acaataatta tg
//
LOCUS       DAOSTA010000063         4025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_20131_length_4025_cov_8.618388, whole genome shotgun sequence.
ACCESSION   DAOSTA010000063 DAOSTA010000000
VERSION     DAOSTA010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20131_length_4025_cov_8.618388"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..646)
                     /locus_tag="PLU08_04805"
     CDS             complement(71..646)
                     /locus_tag="PLU08_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71041.1"
                     /translation="MNVKVVYPAKNIRHDIRRAAIYWAKPAFILAAIISAVVNILVKG
                     SAWSVIVIWSLWMIWSFVFTPTLIEHNRTSIAVKSSIHVTILIVIIYMIYPSWPGIEV
                     ASLVVVGGLIITAILFFSNVSRQKQNIFPLLIFIIIAIIFSVVAFVLRGQEPLGWAMI
                     VSISVALAIFLSTAIILRINFFRELKKRFML"
     gene            complement(609..2129)
                     /locus_tag="PLU08_04810"
     CDS             complement(609..2129)
                     /locus_tag="PLU08_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71042.1"
                     /translation="MRKRKINEFTEYLNHLNADDVQKVKAFMVNFKQHMNLPLSEEKK
                     LRKDLKGALMYYNAIGVSVTEALRRLDNENLGGFYARPPLLYYALDDAAKIYPLSMKH
                     GFQAVFRLSANLYDEVVPQLLQIALTFTIKRFPRFATTLKKGFFWHYLDANKQRYGIE
                     KEEHLPCRPIKVATTGSKSFRVLYYKNRISVEFFHSLTDGKGGIVFLQTLLDTYLRLF
                     GVEGHKHPSVLDINAAPLPEEDANEFKRTTVTKRSGFKEKRALQMSGKIESIRPTQIL
                     HFRLDAKELAKTAKSYDVTITTYVLALILLASRFATDAKEGDIAIQVPVDLRKYYESK
                     TLANFSLYGGIRIPITSITTMEQLLPLVKEQLVTATSHEKMSEMMYSAHRMNKDVRGF
                     PLFLKSPIAKLAYGLMGEAIFTTTLSNLGVVSAPDYFSDYIESYDFVLGPPEVNRAAL
                     TLITYNNIATLTITKSTKDPSFEEEIHRLLTKDGLDITIEGSAVYERKSRVSRKKY"
     gene            complement(2188..2538)
                     /locus_tag="PLU08_04815"
     CDS             complement(2188..2538)
                     /locus_tag="PLU08_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71043.1"
                     /translation="MQILFIVLNDLSYLDKILEKFLDLHVRGATIIDSQGMAAAIMQN
                     EGLLSLLSGPFARSLETNQKNSKTIFTVIPEAEKVTEVVKAVQDIISDCKKQVVGFMF
                     TLPVSGIYPLKPKK"
     gene            complement(2541..3887)
                     /locus_tag="PLU08_04820"
     CDS             complement(2541..3887)
                     /locus_tag="PLU08_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOQ71044.1"
                     /translation="MDAIFVLILKLAIVLSVGFIGSLVARKLKLPNVSGYLLFGLLLG
                     PSLGLIFKGFPGIITVEDSGNLNFISQIALAFIAFSIGAEFSIKSIKKVGKPILVMTS
                     TEVLGAVLVVFFAMFFLPKPQAIMPDGYNPFSKPNVAFGLILASMSAATAPAATLMVI
                     RQYRAFGPVTRAILPITALDDIFGIVVFGFFISFAQILASTGPLPPVWLMILKPFIEV
                     FGSVALGMIIGLLLSLLANKFDRIRDDLQVLSLIAVFGTIGLTYLINYYLEPHGLSFS
                     QLLSNIMIGSMIANIAKRPEKTFNAINDFATPFYILFFTLAGAGLDLAILGTTPLLLL
                     IAGVYILARGFGKWLGIFVGANVVKAEPTVKKYLGIALLPQGGVSIGLLAIVYTQMAP
                     MYPAISTVIMLSVLVYETMGPVFAKFAISKAGEIGGLDRLEQLSSLDGIELTEEGA"
BASE COUNT         1302 a          757 c          773 g         1193 t
ORIGIN      
        1 aacccgcacg gttagcttgg gaagctaaag ttctaccact aaactacagc tgcgtttttt
       61 ctacaataaa ctataacata aatcgctttt ttagttcacg gaaaaagtta attcttaaaa
      121 taattgccgt acttaaaaat atagcaagtg cgacactgat agaaacaatc atcgcccagc
      181 caagtggttc ttgaccgcgt aatacaaagg ccacgacact aaaaataata gcgataataa
      241 taaagattaa aagcggaaag atattttgct tttgacgtga gacattacta aaaaataata
      301 tagcggtaat aattaaacca ccaacgacca cgagtgaagc aacttcgatt cccggccaac
      361 ttggataaat catataaatt atgacgataa gaatagtgac atgaatcgaa gacttcacgg
      421 cgatacttgt gcgattgtgt tcaataagtg ttggagtaaa cacaaatgac catatcatcc
      481 ataagctcca tatgacaatc acgctccagg ccgagccttt cactaagata ttgacaacag
      541 cgctgataat tgccgctaaa atgaaggcag gctttgccca gtaaatagcg gcacgacgaa
      601 tgtcatgtct aatatttttt gcgggataca cgacttttac gttcatatac agcactccct
      661 tctatcgtta tgtctaaacc atcttttgta agcagacgat gtatttcttc ctcgaaggat
      721 ggatctttcg tacttttcgt aattgttaaa gtagcgatat tattataagt gattaatgtt
      781 aatgctgcgc gattaacttc aggtggacca agcacaaagt cataactttc aatatagtca
      841 ctaaaataat ctggggcact tacaacccct aaattactta atgttgtcgt aaagattgct
      901 tcacccatca gtccataggc aagtttagca atcggtgact ttaagaaaag cgggaaaccg
      961 cggacatctt tattcatccg gtgtgcggaa tacatcatct cactcatttt ttcatgactt
     1021 gtggccgtta ctaattgttc cttgacaagt ggtaaaagtt gttccattgt agtaatactt
     1081 gtaattggaa ttcttatccc accataaagc gaaaaattag cgagagtttt agactcataa
     1141 tatttgcgta aatcaacagg tacttgaatt gcaatatcac cttccttagc gtctgttgca
     1201 aagcgacttg ctaaaaggat tagcgctaag acataagttg taatcgtgac atcatatgac
     1261 ttggcagttt tagctagttc tttcgcatca agtctaaaat gcaatatttg tgtcgggcga
     1321 atgctttcaa ttttaccact catttgtaag gcccgttttt ctttaaatcc gctgcgcttt
     1381 gtgacagttg ttcttttaaa ttcgtttgca tcttcttcag gaagtggtgc ggcattaata
     1441 tcgagtacac ttggatgctt gtgtccttca acaccaaaaa gacgtaaata agtatcaagt
     1501 agtgtttgta aaaagacgat tccgcccttt ccgtctgtta acgaatggaa aaattcaaca
     1561 ctaatccgat ttttataata taaaacgcgg aatgattttg aacctgttgt agcgacttta
     1621 atcggacgac atggtaaatg ttcttccttt tcaataccgt agcgttgctt attagcgtct
     1681 aaatagtgcc agaagaaacc tttttttaag gttgtggcaa agcgcgggaa gcgtttaatc
     1741 gtgaaggtaa gcgcaatttg taaaagttgc ggtacaactt cgtcatatag atttgcagat
     1801 agtcgaaaca cagcttgaaa gccgtgtttc attgataaag gataaatctt tgcggcgtca
     1861 tcaagcgcgt aatataaaag agggggacgt gcgtaaaatc ccccaaggtt ttcattatca
     1921 aggcggcgca gagcttcagt tactgataca ccaatagcgt tgtaatacat cagagcccct
     1981 ttaaggtctt tccgtaattt cttttcttcg ctaagtggta agttcatatg ttgtttaaag
     2041 ttcaccataa aagctttcac tttttggaca tcatcagcat ttaagtgatt taaatattcg
     2101 gtaaactcat ttattttgcg ttttctcata atgatagtaa ttataacacg attatgtaat
     2161 cgtgaattta ttgatgttta gatgatttta ttttttaggc ttaagtggat aaataccgct
     2221 tactggcaag gtaaacataa agcccacaac ttgtttttta caatcagaaa taatgtcttg
     2281 gacggctttt acaacttccg tcactttttc tgcttctgga ataactgtaa agattgtttt
     2341 tgagtttttt tggttagttt caagtgatct cgcaaaagga ccggagagca atgagagtaa
     2401 gccttcattt tgcataattg ctgcggccat tccttgtgag tcgataattg tcgcaccgcg
     2461 cacatggaga tctagaaact tttctaagat cttgtctaag taagataagt catttaaaac
     2521 aataaataag atttgcataa ttatgctcct tcctcggtta attcaatacc gtcgaggctt
     2581 gataattgtt caaggcgatc aagacctcca atttcgccgg ctttactaat ggcaaatttt
     2641 gcaaagactg gacccatcgt ttcatacact aatactgata acattataac agtagaaatg
     2701 gcaggataca tcggggccat ctgtgtgtag acaattgcca gtaatccaat tgagacacca
     2761 ccttgtggta aaagggcaat gcctaagtac tttttcaccg ttggttcagc tttaaccaca
     2821 ttagcgccta caaagatacc aagccactta ccaaagccgc gcgctaggat atagacacca
     2881 gcgattaata gtaagagcgg ggttgttcct aaaatggcta aatcaaggcc tgcaccagca
     2941 agggtgaaga aaagaatata aaatggggta gcaaagtcgt taatcgcatt aaatgtcttt
     3001 tcgggacgtt tggcaatatt agcgatcatt gacccaatca taatgtttga taataattga
     3061 ctaaatgata aaccgtgtgg ttctaagtaa taattaatca aatatgttaa tccgattgta
     3121 ccaaagacag caataagtga taagacttgc aagtcatcac gaatgcggtc aaacttattc
     3181 gctagaagcg ataagagtaa accgattatc attccaaggg caaccgagcc aaacacttca
     3241 ataaatggct ttaaaatcat gagccacact gggggtaaag gaccagttga cgctaagatt
     3301 tgcgcaaaag aaataaagaa cccaaagaca acaataccaa agatatcatc aagcgcggta
     3361 attggtaaaa tagcgcgtgt cacaggacca aaagcccgat attgccgaat aaccataagg
     3421 gtagcggctg gggcagtcgc cgcactcatt gacgctaaaa ttaaaccaaa ggccacatta
     3481 ggtttgctaa atgggttata accatcaggc ataattgctt gtggttttgg taaaaagaac
     3541 attgcaaaga atacgactaa taccgcacct aatacttcag ttgaagtcat aactaaaatt
     3601 ggtttaccga cctttttaat tgatttgata ctaaattccg caccaattga aaaagcgata
     3661 aacgcaaggg cgatttggct tataaagttg agattgccac tatcttcgac agtgataatg
     3721 cccggaaagc ctttgaaaat aagaccgagt gatggtccaa gtaacaaacc gaaaagtaaa
     3781 taaccgctaa cattcggtaa ttttaatttt cgggccacga gtgatccgat aaatcccacg
     3841 gatagtacga tggcaagttt taggattaaa acaaaaattg catccataat tttctcctct
     3901 cataaaaata attaatgttc ttaattactc ctatgaggtt agctgtcggg ttcaggacat
     3961 taagaatatc ctggatagat gaataattaa aattaacaag attaagtgtt ttaatgattt
     4021 tatgt
//
LOCUS       DAOSTA010000064         3993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_20325_length_3993_cov_10.407567, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000064 DAOSTA010000000
VERSION     DAOSTA010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20325_length_3993_cov_10.407567"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            619..1572
                     /gene="fmt"
                     /locus_tag="PLU08_04825"
     CDS             619..1572
                     /gene="fmt"
                     /locus_tag="PLU08_04825"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOQ71045.1"
                     /translation="MKDLRIIYLGTPDFSAELLRYLIEAGYNIIAVVTQGDSPVGRKR
                     VLTPSPVKTLALAHNIPVFTPLRIRKEYDFIVELEPDLILTFAYGQIVPKAVLEAPKY
                     GCLNFHGSILPKYRGASPIQMALINAEEETGVSLMEMVEAMDAGRVFGIEKFKIEPSD
                     NFLTIASKMVKASATLIEEVLPSVISGENKGVQQEESQVTFAPLLKIKDEWINLENDD
                     VKNVLGRIQAFAPATGALLSYDDLPLKIYEARFDNNNVTRTIGELFISEKKLLLQVKG
                     GQIEVLKLQKSGKKIIDARSFINGEKHKLPTILKGYIDAEI"
     gene            1559..3889
                     /locus_tag="PLU08_04830"
     CDS             1559..3889
                     /locus_tag="PLU08_04830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOQ71046.1"
                     /translation="MRKFNLSVHELVDFVLHSGDLDTRIFNLETMTRGTQIHAFYQAK
                     QNDKYLAEYALEYTFEYENHLITLQGRVDGVIVNGSEVTIEEIKSANTDIDKFFENNE
                     AWHLGQAKCYALMYALRFNLDVIDIQLTYISQIDERKMMKRYTFTREELQSDVFSYFG
                     EYLAFFYQVEKYEKRRTKRLKALNFPYKEVRSSQKVMMEQVEHALEHEHKIFLNAPTG
                     SGKTMAVLYPSLKVLAEKTNTKIFYLTAKGSGKAQAENALRVVREKALVKSTTVSAKE
                     KMCLNDKVACNPDECPYAVNYYGKIRNALLDAFKSRDEYNTEIVTEYAKKHELCPFEF
                     QLDLTLLSDVIIADYNYLFDPFVYLRRFFDSKRGNYIALVDEAHNLPRRVSDNYSLVL
                     DFTPFYSFRKVLKGAEHKKIRRIINRIVKDLDEYKEGIISEYETITEFPHELRSSLDR
                     FLVVGSEYMQEQGNNLAEGFSDFFFDVNKFIKILDLAYEDFILYTEIDEARKFFPKFF
                     IKCLSGAKFINEFLKVIDHAIFFSATLAPAPYFKTMLSIDDDIHQIPNPFSREQLLIM
                     VDGSTSLYYKDREQTLSVVAEKIRAAISVKTGNYIVYTPSFMYLENLEQELRNSEDYD
                     LIVQRPSMSDIEKEKYLETFKLSPTKTTLGLAVLGGSFSEGVDLVSDRLSGVIILGVG
                     YPPPSFEKELEKDYFENKLNDGISYAYVYPALNKILQTMGRVIRSEKDYGFILLMDKR
                     YNYSPYRQQLESYDGLVVQVKSAERISHLLARFFLN"
BASE COUNT         1372 a          599 c          764 g         1258 t
ORIGIN      
        1 caataatttt tcagtaattg cgcgtttcta ttcattatat tcattaaatt tcttcaaagt
       61 aggcaataga gtaatttata gcgatactat gattaaaaaa actttattta aagtttaagg
      121 cgttttctcc ttgcaaaaaa gtcatgcaag taataaaaaa taccaagtag taaagatatc
      181 ccaaatatta taagtatgac gacactagcc ttcatatcgt aagcattcca tccgagtatt
      241 agcggaacag aagcatagat aaaaaagcgc cgtatggata agcattgtaa tgtgaataca
      301 aggaacgtgt aattccttat cttttaaaaa taaaataagt agtgacaatg taatcattat
      361 tgcccctagc acatatatta agataactgg atctttaaaa aatatataaa taacgagaat
      421 tgtcgtccat aacaccattg aatataaggc aaagataatt tttttaattc ttttaggcat
      481 atgtttattt ttcctagagt tcaacatatg ttttaaatta tagccattaa agctagtaaa
      541 ttcaatatta tgggacgagg atttaatata tttcattacg aagtgtgaat ttttgtctat
      601 aatttaagcg tgaagagtat gaaagattta agaattattt atttaggaac accagacttc
      661 agcgctgaac ttttacgcta tttaattgaa gcaggttata acattatcgc cgttgtcaca
      721 caaggtgata gtcctgttgg gcgcaaaaga gtattaacac caagtcccgt aaagacttta
      781 gcattagcgc ataatattcc cgtttttact ccgcttcgca ttcgtaaaga atatgatttc
      841 atagtggaac tagaaccgga cttaatcctt acttttgcct atggtcaaat tgtacctaaa
      901 gcagtactag aagcacctaa atatggttgt ttaaattttc acggctcaat tttgccaaaa
      961 tatcgcgggg cttctccaat tcaaatggca ctaataaatg ccgaagaaga aacgggtgtt
     1021 agtcttatgg aaatggttga agcaatggac gctggccgag tgttcgggat tgaaaaattt
     1081 aaaattgaac caagcgataa cttcctaaca attgcaagta agatggtaaa agcaagtgcg
     1141 acactgattg aagaagtttt accaagtgtt attagcggcg aaaataaagg tgtgcagcaa
     1201 gaggaatcac aagttacttt tgccccgctt ttaaagatta aagatgagtg gattaatctt
     1261 gaaaatgacg atgttaagaa tgttttaggg cggatacaag cttttgcgcc cgctacaggt
     1321 gcgttattaa gttacgatga cttaccatta aaaatttacg aagcacgctt tgacaataat
     1381 aatgttactc gcactatcgg tgaacttttc attagtgaga aaaaactttt attacaagta
     1441 aagggcggac aaattgaagt acttaaactg caaaaaagcg gcaagaaaat aattgatgct
     1501 cgtagtttta ttaatggtga aaagcataaa ttaccgacca ttttaaaagg atatattgat
     1561 gcggaaattt aacctcagtg ttcacgagct cgtggacttt gttttacatt ctggggattt
     1621 agatacccgc atttttaatt tagaaacaat gacgcgaggt acacagattc acgccttcta
     1681 tcaagctaaa caaaatgata aatatcttgc cgaatatgca cttgaatata cttttgaata
     1741 tgaaaatcat cttattacat tacaaggaag agtagacggg gttattgtta atggaagtga
     1801 ggtaacgatt gaagaaatta aaagtgcgaa cactgatatt gataaatttt ttgaaaataa
     1861 tgaagcatgg catcttggac aagcaaagtg ttatgcattg atgtatgcgc tccgttttaa
     1921 cttagacgtt attgatattc aattaacata tatttcacaa attgatgaac gcaagatgat
     1981 gaaacgctat acatttacgc gtgaagaatt acaaagtgat gtttttagtt attttggtga
     2041 atacttagcg tttttctatc aagtcgaaaa atatgaaaag cgccgtacaa agagacttaa
     2101 agcacttaat tttccttata aggaagtacg ttctagtcaa aaagtaatga tggaacaagt
     2161 agagcacgcc ttagaacatg agcataaaat attcttaaat gcaccaactg gtagcggtaa
     2221 aactatggcg gtactttatc ctagtcttaa agttttagcg gaaaaaacta atactaaaat
     2281 cttttactta accgcaaaag gaagtgggaa ggcacaagcc gaaaatgctt tacgagtagt
     2341 gcgcgaaaaa gcactagtta aaagtactac agtcagcgcc aaagaaaaga tgtgtcttaa
     2401 tgataaagtt gcctgtaatc ctgatgaatg tccatatgcc gttaattatt acgggaagat
     2461 tagaaacgcg ctacttgatg cttttaagag tagggacgaa tataacacag agattgttac
     2521 tgaatatgcc aaaaaacatg aattatgtcc gtttgaattt cagttagatt taacactttt
     2581 gagtgatgtt attattgctg attacaatta tctattcgat ccttttgtgt atttaaggcg
     2641 ctttttcgat agtaaacgcg gtaattatat tgcccttgtt gatgaagcgc ataacttacc
     2701 acgccgtgtg agtgataatt actctttagt gcttgatttt acgccgttct atagttttag
     2761 aaaagtgtta aaaggcgcgg aacataagaa aattagacga attattaatc ggattgttaa
     2821 agaccttgat gaatataaag aggggattat tagtgagtat gaaacaatca ctgaatttcc
     2881 acatgaatta cggagtagtc ttgaccgctt tttagttgta ggtagtgaat atatgcaaga
     2941 acaaggcaat aacctagcgg aaggctttag cgatttcttt tttgatgtga ataaatttat
     3001 aaaaattctc gatttggcgt atgaagattt tatcctttac accgaaattg atgaagcacg
     3061 gaagtttttc ccaaagtttt ttattaagtg tcttagtggc gcaaagttta ttaatgaatt
     3121 tttaaaagta attgaccacg cgatattttt tagtgcgaca cttgcaccag cgccttactt
     3181 taaaacgatg cttagtattg atgatgatat tcatcaaatt cctaacccgt ttagccgtga
     3241 acagctttta attatggttg acggtagcac tagtctttac tataaagacc gcgaacaaac
     3301 tctaagtgta gtagcggaga aaattcgggc agcgataagt gttaaaactg gtaattatat
     3361 cgtttatacg cccagtttta tgtatcttga aaatttggag caagagttaa gaaatagcga
     3421 agactatgat ttaattgtgc aacgaccaag tatgagtgat atcgaaaaag aaaagtatct
     3481 agaaacattt aaactctcgc caacaaaaac aacacttggt ctagcggtat taggcggctc
     3541 ttttagtgaa ggagtcgacc ttgttagtga ccgcttaagt ggtgtaatta ttctaggagt
     3601 agggtatccg ccaccatctt ttgaaaaaga actagaaaaa gattattttg aaaataaact
     3661 taatgacgga ataagttatg cttatgttta tcctgcgctt aataagatac ttcaaactat
     3721 gggccgcgta attagaagtg aaaaagatta cggttttatc cttttgatgg ataagcgtta
     3781 taattacagt ccctatcgcc agcaattaga aagttatgac ggcctagtcg tgcaagtaaa
     3841 aagtgcggaa agaatcagcc accttttagc gcgttttttc ttaaattaac tcactatttt
     3901 taagtgctta tatgaatgtt ttgtacttag tcttttctca tggatagatg aataattaaa
     3961 attaacaaga ttaagtgttt taatgatttt atg
//
LOCUS       DAOSTA010000065         3593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_23315_length_3593_cov_9.194743, whole genome shotgun sequence.
ACCESSION   DAOSTA010000065 DAOSTA010000000
VERSION     DAOSTA010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23315_length_3593_cov_9.194743"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLU08_04835"
     CDS             complement(<1..247)
                     /locus_tag="PLU08_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71047.1"
                     /translation="MHKGKRNFFAIVLTLLIALGLPKLSSNSTRDKVNASATETVYLK
                     VNDNWKIDNARFAAWVWNPGTWFDLTQVEGETHIYKAD"
     gene            601..915
                     /locus_tag="PLU08_04840"
     CDS             601..915
                     /locus_tag="PLU08_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71048.1"
                     /translation="MVKRDHPDWCLKGPTEITKEEYNELKAKLVNDPYWNKKTRQNTA
                     LFADKLIDEPNYTNRRGIKTNTATFKKPTTAEIKMFHQKKRKIRDKEIQEFYDEANDY
                     ES"
     gene            complement(970..1536)
                     /locus_tag="PLU08_04845"
     CDS             complement(970..1536)
                     /locus_tag="PLU08_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71049.1"
                     /translation="MKRIHYYIVGTIILFATVIFSNSVGATSGANKRMEKLEVKARDL
                     FVTLSERYTKIEELHDNLSALDETATALVNNIEERITNFNDTKLLSQTVWPALQDAKY
                     IDENFMTLILYLKDNKDTFGSTLPSSFYTEFDAITSVLLPKINAYNSVTADYNTHIDL
                     FPNRLYLKGRNPYIIFDLALYPENLPLF"
     gene            complement(1551..>3593)
                     /locus_tag="PLU08_04850"
     CDS             complement(1551..>3593)
                     /locus_tag="PLU08_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71050.1"
                     /translation="SGYLINLYRIHGFNQENNEVESTIENVADLPSESENPLNLSYFL
                     DSIAIPKIVTLKDLGDVTFEVTTNRPDLLFIGEMSKPIEDLDPGNELITGDVTVHTLT
                     PVGTYSSEIPASVDTVDKLKDHLKSVDVKELYPYATQPTQDFSITITATCFGEERTET
                     YYFRLQEPPLDQEIVDYAIKSEYMINKVFYKDTTSIIRTTRENMPSTPEKRMKVSYLT
                     ETLAIPRHVVLSDFGNKELDFSINVLGNNADKFYMSEQTDTLDGNPVTVKTLTPVGGL
                     AYTTPQNDLPAFAEEIKGLSREDLYDCVTQTSIDVDIELSATLNGKTATKTYYFTLVA
                     ADIDVEIIDYLIDDSSLLNVVTYEDVNDDVKIQKIADQAKDPLNPYAIEHLVDTIVVP
                     QSITLTNFGNRELQIETTINSDDPGALNFYEASKSATISDNLVTAKTLTPLGSYDASA
                     VDPNNLTALADSIKALPTKDLYKHSQSVGYNVELNVTVTLVSGEGEPEVRSTTFYLKT
                     IKADVDSKIGTAILDTYDIVNLIDHTDPYINVTNPPQPTDPLNPHQLLYFGETLVFPI
                     EFEYTDFENKLIQFDVNTNGKDHLFFISQKTHHYEGKDVEGITLSPIGTYDPGEPLNT
                     YQDLINHLKNDANIMAIAAARAVQVEENVTITVTISIDGREVAVNNYYFVLVVERS"
BASE COUNT         1144 a          620 c          654 g         1175 t
ORIGIN      
        1 tatcagcttt ataaatatgc gtctcgcctt caacttgcgt taaatcaaac caagtacctg
       61 ggttccacac ccatgctgca aagcgggcat tatcaatctt ccagttatca ttaactttta
      121 aataaactgt ttcagtagcg cttgcattaa ctttgtcacg cgttgagtta ctacttagtt
      181 taggtaatcc tagcgcgatt aaaagcgtta agacaatagc gaagaaatta cgttttcctt
      241 tatgcataat cgttcctacc ttctttgtac agattagttt ttgtgaagta gtaagtaaat
      301 taataagtaa tttacttttt aattaaaatt taattcatca cacttatctt taatattacc
      361 atattaaaca aatatcacac cttaaattta gcatgtgtat tcaaaaatac gcctattagt
      421 gttagaataa gagtgaattt aatattagtt aggagtaatt atggcctttt ttgccgagcg
      481 caaatattat gttgttacct gtaaatttgg tcacgttggt cgtgataagt atttaccact
      541 tgatacctat tagagcaaca agtaaaaaag aagcaagcgc taaagcaaaa aagactgggg
      601 gtggtaaagc gcgatcaccc tgattggtgt ttaaaaggac caacggaaat tactaaagaa
      661 gaatataatg aacttaaagc aaaattagta aatgatcctt attggaataa gaaaacacgg
      721 caaaacaccg ctctttttgc tgataagctc attgatgaac ctaattatac gaataggcgc
      781 ggcattaaaa caaatacagc tacctttaaa aaaccaacaa ccgctgaaat aaaaatgttt
      841 catcaaaaaa agcgcaagat tcgtgataaa gaaatccaag aattttatga tgaagcaaat
      901 gattacgaaa gttaaaaaat aaaatccacc tagtctatta gtaaggacac taaaaggtgg
      961 gtttttttgt tagaaaagtg gtaaattttc tggataaagc gctaaatcaa agataatata
     1021 cggatttcgt ccttttaagt agagtcggtt cgggaaaaga tcaatatgtg tgttgtaatc
     1081 agcagtcaca ctattataag cattaatctt tggtaataaa acacttgtta ttgcatcaaa
     1141 ctctgtgtag aaggaagaag gaagggtact accaaaagta tccttattat cctttaaata
     1201 aagtatgagt gtcataaagt tttcatcaat atacttagca tcttgcaatg caggccacac
     1261 agtttgagag agaagcttag tgtcattaaa attagtaatc cgctcctcaa tgttatttac
     1321 taaagcagta gcggtttcat ctaaagcgga taaattatca tgtaattcct ctatttttgt
     1381 gtagcgctca cttaaggtaa caaataagtc gcgcgctttt acttcaagtt tttccatgcg
     1441 tttatttgct ccgctcgtag cgccaacgga gttactaaaa ataactgtcg caaaaagaat
     1501 aattgttcca actatataat aatgaattcg cttcatttaa tcgtctcttt ctaacttcgc
     1561 tcaacaacta agacaaagta gtaattattg acagcaactt cgcggccatc aatcgatatt
     1621 gtaacagtta ttgttacatt ttcctcaact tgtactgctc tcgctgcggc aattgccatg
     1681 atattagcat catttttcaa gtgattaatt aagtcttggt aagtgttaag tggttcacct
     1741 ggatcataag tgccaattgg gctaagtgta atcccttcaa catctttacc ttcatagtga
     1801 tgggtttttt gtgagatgaa gaacaaatga tctttaccat ttgtattaac atcaaactga
     1861 atgagcttat tttcaaagtc agtatattca aattcaatcg ggaacacgag tgtttcacca
     1921 aaatagagta attgatgtgg attaagcgga tcagttggtt gtggtgggtt agtgacatta
     1981 atatacggat cagtgtggtc gataaggtta acaatatcgt aagtatctaa aatcgctgtc
     2041 ccaatcttgc tatcaacatc agccttaatt gtttttaagt agaatgttgt ggaacggact
     2101 tctggttcac cctcgccact aactaaagta acggtgacat taagttcaac attataacca
     2161 accgattgtg aatgtttgta taagtctttt gttggtaagg ctttaatact atcagcaagc
     2221 gctgttaagt tatttggatc gacagcacta gcgtcataac taccaagcgg tgttagtgtc
     2281 ttagcggtca ctagattatc actaattgtt gcgctcttac tagcttcata gaaattaagc
     2341 gcaccaggat catcactatt aattgttgtt tcgatttgta actctctgtt gccaaagttt
     2401 gtgagggtaa ttgattgcgg gacgacaatt gtatcaacga gatgttcaat cgcgtaagga
     2461 ttaagtgggt ctttagcctg atcagcaatt ttttgaattt ttacatcatc gtttacatct
     2521 tcataagtaa cgacattgag taaagaacta tcgtcaatta agtaatcgat aatttccaca
     2581 tcaatatctg cggccacaag tgtgaaataa tatgtttttg tcgcggtttt accgttaagt
     2641 gtagcactta gttcaatatc aacatcaata ctagtttggg taacgcaatc ataaagatct
     2701 tcacgcgaga gccctttaat ttcttcggca aatgctggta aatcattttg cggtgtcgtg
     2761 taagctaaac cgccgactgg tgttaatgtc ttaacagtga cagggttacc gtcaagcgta
     2821 tcagtctgtt cggacatata aaatttatca gcgttattac ctaatacatt aattgagaag
     2881 tctagctctt tgttaccaaa gtcacttaaa actacatggc gcggtatggc tagagtttcg
     2941 gttaagtacg ataccttcat ccgtttttct ggagtggaag gcatattttc acgtgtcgta
     3001 cgaataatac ttgtggtgtc tttgtaaaat actttattaa tcatgtattc gcttttaatt
     3061 gcataatcaa caatttcttg gtcaagcggt ggttcttgta gcctaaagta ataagtttct
     3121 gtccgttctt caccaaaaca agtcgcggta atggtgatgc taaaatcttg agttggttgc
     3181 gtagcgtaag gatataattc ttttacatcc acactcttta agtgatcttt taatttatcg
     3241 acagtatcga cacttgcggg aatttcacta ctgtaagtcc caacaggtgt taaagtgtgc
     3301 acagtaacat cccctgtaat taattcatta ccgggatcta aatcttcaat tggcttactc
     3361 atttcaccaa taaataataa atcaggacga ttcgtcgtga cttcaaaagt gacatcgccc
     3421 aagtctttaa gtgtaacaat ttttggaatg gcaattgaat caaggaaata agaaagatta
     3481 agtggatttt cgctttcact tggtaagtct gcgacatttt cgattgtact ttcaacttca
     3541 ttattttctt ggttaaaacc gtgaatacga taaagattaa ttaagtaacc aga
//
LOCUS       DAOSTA010000066         3410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_24935_length_3410_cov_12.881967, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000066 DAOSTA010000000
VERSION     DAOSTA010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24935_length_3410_cov_12.881967"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1400)
                     /locus_tag="PLU08_04855"
     CDS             complement(51..1400)
                     /locus_tag="PLU08_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71051.1"
                     /translation="MPELEKKLHETKTLIPEVPQRLDIKMEERIDGATKVKRPLNLRF
                     RFVLATAIVACVALSATTIALFSHTQKEQQQLFHKLPQSKVLMNLDDDLVNRTAIKAV
                     EKLSPFLTDEKAEDTVLSPATYSLAMAGLSAVSDNFNNDAFGLINAEEDVKALLEAWN
                     FKFKNYTGETFFRSAVLHQQVGPTYAFDKSKQEAVSDHYISTMVSSHESYRKDADKFF
                     DKKIGLKLKVPDLNLKSDGVVTYGVLKMKDYVANDGLFHRHMPFTFKSGLGTADSYVF
                     AVREDPKKVAYYRGGNYEVFKVNIRNTDLMIVLPNEEVNINHVNITEAYTNFVNEAEL
                     VPAFGYVPFFHNKVEGLNLTDTLENVITHNEIYMSKLLKDDVYNDLVIDAVVQSNDFE
                     FNKYGVAGESMTAVSGCTSTGPLYDEPIDLSVNRPFYAFSLKDKYPIFINKVSKPGA"
     gene            complement(1393..1866)
                     /locus_tag="PLU08_04860"
     CDS             complement(1393..1866)
                     /locus_tag="PLU08_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOQ71052.1"
                     /translation="MTKEMFSEKYLTYKVMVFQISYAYLRNIEDAEEATLDSFMKFYK
                     STKKFQSVNHEKNYLIRVTINTAKDYLKKIKALPLNEEVHGVYDDNGELDKHYVWAVV
                     DELEGKYKDVIYLRYTADLPYKDIASILKISEALARKYHEIAIKTLRELIGGNYA"
     gene            complement(2035..2970)
                     /locus_tag="PLU08_04865"
     CDS             complement(2035..2970)
                     /locus_tag="PLU08_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008753856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOQ71053.1"
                     /translation="MSKINEIQVLLSKKAELNARLSLLPYDGTPEIKTISGKDYLYVR
                     KRVLNKVTSTYVDVYSDKLYNNLLRNNEEAKKLRREIRKIDKALASFGYKEGILLPNV
                     ILARDFARKNRNAIIYDQVILEGIGTTFPETEAILEQGIIKNARAEDVQKILNLKRAW
                     EFILDDDVLLTKTDFHLISYIAKIVNEGLFIFGGEVRGVSVKIGGSTYYPPIPLESKV
                     KEDINALLSSSLNDVDTAIELCLYIMKTQIFNDGNKRTAVIATNHYLVSKGIGFLAIA
                     YDKVPAFREMLVNYYENKNINEIKNFLKSCIHLVD"
     gene            complement(3161..3237)
                     /locus_tag="PLU08_04870"
     tRNA            complement(3161..3237)
                     /locus_tag="PLU08_04870"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3201..3203),aa:Arg,seq:acg)
BASE COUNT         1076 a          634 c          495 g         1205 t
ORIGIN      
        1 cattttgatg tagccttatt tttagtaaat agttttatga caaacaataa ttatgcgcca
       61 ggctttgaca ctttattaat aaagattggg tatttatctt ttaaagagaa ggcataaaac
      121 ggacgattaa cagaaagatc aattggttca tcgtaaagtg gaccagttga tgtacagccg
      181 gaaactgcgg tcattgattc tcctgccaca ccatatttat taaattcaaa gtcatttgat
      241 tgcacaacag cgtcaataac taaatcatta taaacatcat ctttaagtaa tttactcata
      301 tagatttcgt tatgcgtaat aacattttct aatgtgtctg ttaaatttag accttcaact
      361 ttgttatgga agaagggtac atagccaaaa gcgggaacaa gttcagcctc attaacaaaa
      421 ttagtgtaag cttcagtgat atttacatga ttaatattaa cctcttcgtt tggtaaaact
      481 atcattaaat cagtatttcg aatatttact ttaaatacct cataattacc tccgcgataa
      541 taagcaactt tctttggatc ttctcttaca gcaaagacat aggaatccgc tgtgcctagc
      601 ccgcttttaa atgtaaaagg catatgtcga tgaaaaagcc catcgttagc gacataatct
      661 ttcattttta aaacaccgta agtcactact ccatcacttt taagatttaa atcaggaact
      721 ttaagcttta aaccaatctt cttatcaaaa aatttatcag cgtctttacg gtaagattcg
      781 tgcgaagaga ccattgtcga aatgtaatga tcacttactg cttcttgttt actcttatca
      841 aaagcataag ttggtccaac ttgttgatgt aagactgccg agcggaaaaa agtttcacct
      901 gtgtaatttt taaatttaaa attccatgct tctaagagcg cttttacatc ttcttcggca
      961 ttaattaaac caaaagcatc attattaaag ttatcactaa cagcggaaag cccagccatc
     1021 gctaaggagt aagttgctgg tgatagcaca gtatcttctg ctttttcatc agtaagaaag
     1081 ggggataatt tctcaaccgc cttaattgct gttctattta ctaagtcatc atctaaattc
     1141 atcagcactt tagactgtgg taatttgtga aataattgtt gttgttcttt ttgagtatga
     1201 ctaaataagg caattgtcgt cgcgcttaaa gccacacacg cgacaatggc tgtcgctagg
     1261 acaaagcgaa aacgcaagtt aagcggacgc tttacttttg tagcaccatc aatcctttct
     1321 tccattttaa tatctaatct ttgtggcact tcaggaataa gtgtttttgt ttcatgtaat
     1381 ttcttttcta attcaggcat aatttcctcc gattaattca cgcaatgttt taatggcaat
     1441 ttcatgatat ttcctcgcta gtgcttcact tattttaaga atgcttgcta tatctttata
     1501 cggtaagtct gcagtataac gcaaataaat aacatcttta tatttacctt ctaactcgtc
     1561 gactactgcc catacataat gcttatcaag ttcaccatta tcgtcataca caccatgaac
     1621 ttcttcattt aagggtaacg ctttaatttt ctttaagtaa tcttttgcag tattaatcgt
     1681 tactctaatt aaataattct tttcatggtt aactgactga aatttcttcg tgcttttata
     1741 aaatttcata aatgagtcaa gcgtcgcctc ttcagcatct tcaatattcc gtaaatatgc
     1801 atagcttatt tgaaacacca tgactttgta ggttagatac ttttcactaa acatctcttt
     1861 agtcataagc acctacacta ttattacgat taacgcgaca cttttgtgaa ttcatcaaca
     1921 ctttatttgt ttccattatt attaatacct actacttata atacgattaa aaacactgtt
     1981 ttgcgaaaaa atagtaaata ttgttaaaaa accctttata ttgctaagtt tgcactagtc
     2041 aactaaatgt atacaacttt taagaaagtt tttaatttca ttaatatttt tattttcata
     2101 ataatttact aacatttctc taaaagcggg aactttatcg taagctattg ctaaaaaccc
     2161 aatgccttta ctcactaaat aatggttagt ggcaataacc gcagttcttt tattgccatc
     2221 attaaagatt tgcgttttca taatgtataa acaaagttca atagcggtat cgacatcatt
     2281 taacgatgaa gacaataaag cgttgatatc ttcttttact ttagattcaa gtgggatagg
     2341 aggataataa gttgaaccac caatcttaac agatacccct ctaacttcgc caccaaaaat
     2401 aaataatcct tcattaacaa ttttagcgat ataactaata agatgaaaat ctgttttcgt
     2461 taataaaaca tcgtcatcta aaataaattc ccacgctctt ttcaaattaa gaattttttg
     2521 cacatcttcc gctctagcat tcttgataat accttgttcg agaattgctt ctgtctcggg
     2581 aaaagttgta ccaattccct ctaatataac ttgatcataa ataatcgcat tacgattttt
     2641 tcgcgcaaaa tcccgcgcta aaatgacatt aggtaatagt attccttctt tgtaaccaaa
     2701 actcgctaaa gctttatcga ttttgcgtat ttcacgccta agtttttttg cttcttcatt
     2761 attacgaagt agattgttat aaagcttgtc actataaaca tcaacatagg ttgaagtcac
     2821 tttatttaga actctttttc gcacatagag ataatctttg ccgctgattg tttttatttc
     2881 aggagtgcca tcatacggca ataacgatag ccgtgcgttt agttcagctt tttttgataa
     2941 taatacttgt atttcattta ttttactcat aacaattctc ttagggaact ttctaactta
     3001 attatacacc ttttgttccc taaaacaaat ctaaatttta ccttgtcaaa tttattcgtt
     3061 tatcgcatgt attagacata attagatttc ataaataaaa agctcccctt ttcattgtac
     3121 tagactctag tgcaatttta gggagcatgt ttattcgcaa tggcgcggtc aggaagaatc
     3181 gaactcccaa cctccagatt cgtagtctgg cactctatcc agttgagcta tgaccgcagc
     3241 gtcacttaaa cattatagtt atttaagtga tttttgcaag tgttataaag ttaaatatgt
     3301 aatatttagc aaataatcag cgtttccctt ggtcataagg ctcacctgcg gcttttggtc
     3361 cgcgtgattt aagtgatgta aaggcaagca caataagtgt tacaacatat
//
LOCUS       DAOSTA010000067         3220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_26995_length_3220_cov_7.831912, whole genome shotgun sequence.
ACCESSION   DAOSTA010000067 DAOSTA010000000
VERSION     DAOSTA010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26995_length_3220_cov_7.831912"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..83
                     /locus_tag="PLU08_04875"
     tRNA            7..83
                     /locus_tag="PLU08_04875"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41..43,aa:Pro,seq:tgg)
     gene            92..167
                     /locus_tag="PLU08_04880"
     tRNA            92..167
                     /locus_tag="PLU08_04880"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:125..127,aa:Ala,seq:tgc)
     gene            177..254
                     /locus_tag="PLU08_04885"
     tRNA            177..254
                     /locus_tag="PLU08_04885"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:212..214,aa:Met,seq:cat)
     gene            271..346
                     /locus_tag="PLU08_04890"
     tRNA            271..346
                     /locus_tag="PLU08_04890"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:304..306,aa:Ile,seq:cat)
     gene            350..440
                     /locus_tag="PLU08_04895"
     tRNA            350..440
                     /locus_tag="PLU08_04895"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:384..386,aa:Ser,seq:tga)
     gene            550..1707
                     /locus_tag="PLU08_04900"
     CDS             550..1707
                     /locus_tag="PLU08_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71054.1"
                     /translation="MKAKIITMLTFCLLMLVSCNDKTSSSNIPSEEVTSTQVTSTPTS
                     TSLPSSEPLEESSDEPLTSEASSNTPSSEQSTINEELQFISNSSRYGNTNGNTMNRSL
                     AVYDFERKMHYYANRGSIYAYNPVTATSTELLADMSSITYLTLYENTIYFIGSTENYL
                     YTYDLLSMTTDLVLERGVSYASRQTNYLYLKTTIEGFGDDFYFVCYNLSNMTMIKYSS
                     MLDNINHVWNKVIYTDSEALKIKLVSYNLSGRTTIANFTERGFKALNYLHLLSEDYVD
                     EHNRLFFVSLQTQEGPAYYFYEIKTDTLTFICKGGAHSVNNDDKYLYLVIDRDIVIYN
                     YVEKTYERTIYFDMSVRNLNVINHYLYYQSGDHNDLYMYHPEMVVPFCLTA"
     gene            2258..2554
                     /locus_tag="PLU08_04905"
     CDS             2258..2554
                     /locus_tag="PLU08_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71055.1"
                     /translation="MLFSRRIFHQYEKPFYSKDGVEITPDWTLPQYKDLGDVIIEYWG
                     ITNDEKYEESKKYKLDIYKKEGVTLISIEQSEIKNLAEILERKLKTAKKGKINS"
     gene            2590..3111
                     /locus_tag="PLU08_04910"
     CDS             2590..3111
                     /locus_tag="PLU08_04910"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOQ71056.1"
                     /translation="MEIIKSLKDSEYPKAALYNARIAARGVCLNAKGEIALTHILATD
                     KFGHRDYYELPGGGKRINETVLESAIREMDEELGVKVELLAKIGIIHDFYNLIKQENY
                     SYYYLFKVTKVGQRHFEERESRLIDKIVWVPIQKAILLYKEQLKIKGVGLLVARRELP
                     ILELVAKSFDKYV"
     gene            3139..3214
                     /locus_tag="PLU08_04915"
     tRNA            3139..3214
                     /locus_tag="PLU08_04915"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3172..3174,aa:His,seq:gtg)
BASE COUNT         1122 a          498 c          624 g          976 t
ORIGIN      
        1 aagcatcggg atgtagcgta gcttggtagc gcgtctgttt tgggaacaga aggtcgcagg
       61 ttcaaatcct gtcatcccga ccatttatta tggggccgta gctcatctgg gagagcgctt
      121 gccttgcacg catgaggtag cgagttcgat cctcgtcggc tccaccatta tattgtggct
      181 gcgtagctca gttggtttag agcggctgac tcatacccag caggtcgggg gttcaaatcc
      241 ctccgcagct accaatacta aaattaagct ggacccttag ctcaatggtt agagctcccg
      301 gctcataact ggttggttat aggttcgagt cctatagggt ccaccatacg gaagcatacc
      361 caagcggttg aaggggtcgg tcttgaaaac cgatagggga tgcaagtccc gcctgagttc
      421 gaatctcagt gcttccgcca gaaaattaat atatgctcat atattgagca ttttttatta
      481 tcatataaaa tgttgtattt ctctaatatt aaaaacgcta caataaattt aaggagaaac
      541 aaaaacttta tgaaagccaa aataattacg atgttaacct tttgtctttt aatgcttgtt
      601 agttgtaatg acaaaacttc aagtagtaat ataccctccg aagaagttac tagtacacaa
      661 gtgacttcaa caccaacatc cacctccctt ccaagtagtg aaccgcttga agaatcaagt
      721 gatgaaccac taacaagcga agcatcaagt aacacacctt catctgagca atcaacaatt
      781 aatgaagaac tgcaatttat atctaattca agtcgttacg ggaacacaaa cgggaatact
      841 atgaaccgta gcctcgcggt atatgacttt gaacgaaaaa tgcattatta tgcaaatcgt
      901 ggtagcattt atgcttataa tcccgtaaca gcgacaagca cagagttact tgcagacatg
      961 tcaagtatta catatttaac gctttatgag aacactattt attttatcgg tagtaccgaa
     1021 aattatttat atacatatga cttattatca atgaccactg atcttgtgct agaacgcggt
     1081 gtaagttatg catcacgcca gactaattat ttatatttaa aaactacgat tgaagggttt
     1141 ggagatgatt tctatttcgt ttgttacaat ttaagtaata tgacgatgat taaatatagt
     1201 agtatgcttg ataatattaa tcatgtatgg aataaagtca tttatacgga tagtgaagca
     1261 ctgaagatta aattagtgtc ttataactta tccggtcgca ccacgattgc taatttcact
     1321 gaacgcggat ttaaagcact taattatttg catttattaa gtgaagatta tgtcgatgaa
     1381 cataatcgtt tattctttgt gtctttacaa acacaagaag gacccgcgta ttacttttat
     1441 gaaataaaga ctgatacact tacatttata tgtaaaggcg gagcacatag cgttaataat
     1501 gatgataaat atctttattt agtaattgat cgtgatatcg tcatttataa ctatgttgaa
     1561 aagacttatg aaagaacaat atattttgat atgagtgtcc gcaatttaaa tgtgattaat
     1621 cattaccttt attatcaaag tggggaccat aatgatcttt atatgtatca cccagaaatg
     1681 gtcgtgcctt tttgcttaac agcatagtag tgaggattga cactgattta actccttaac
     1741 tgggagtttt ttggatttaa taacttaatt tacagtttaa tattagaatc taatctatac
     1801 cagttcatac catgataatg ttatactaat gaaaatagga ggtagaacaa tggaacaaaa
     1861 atgtccaatt tgtggcaaga aaacatcatc ctatattggt aatgtgagaa aagattgatt
     1921 atgtcgagaa catgctaaac aactaaaaga aggaaaaata ataaaatgtg aaaattgtgg
     1981 ttcaattatt aaaagtgatg aaccttgtcc gaattgtgat ccagtaagtg caactgtaat
     2041 aattgattct caaaatactt gcataacatg tggtgaaaga actgatggat atcatttttg
     2101 taaggtttgt tataacaaat ataaagataa aaatataata gtcaatattg caaaatgtaa
     2161 ggaagcgaaa attttagaag aggattacta caacaccata tacaaaaccg aggatggtca
     2221 tctggtaaaa agtaaagatg aggttattat tgataatttg ctttttagta gaagaatatt
     2281 tcatcaatat gaaaagcctt tttattccaa agatggtgtt gagataacac cggattggac
     2341 attaccacaa tataaagatt taggtgatgt cattatagag tattggggaa taacgaatga
     2401 tgaaaaatat gaggaatcaa aaaaatataa attagatata tacaaaaaag aaggagttac
     2461 attaatttca atagagcaaa gtgaaattaa aaatcttgct gaaattttgg aaagaaaact
     2521 aaagactgca aaaaaaggaa aaattaatag ttgagttaat ttaaaatgca tatttagtaa
     2581 atttaaaaaa tggaaattat taaaagttta aaagatagtg agtatccaaa agccgcttta
     2641 tataacgccc gaatagcggc gcgcggtgta tgtttaaacg caaaagggga aatagctttg
     2701 acccatattt tagcaacgga taaatttggt caccgtgatt attatgaatt accaggtggc
     2761 ggtaagcgta ttaatgaaac agtgttagaa agtgctatcc gtgagatgga tgaagagctt
     2821 ggtgtaaaag ttgaactctt agcgaaaatt gggattattc atgactttta taatttgata
     2881 aaacaagaaa attattcgta ttattattta tttaaagtga caaaagttgg tcagcgtcac
     2941 tttgaagaaa gggaatcgcg ccttattgat aaaatcgtct gggttcccat acaaaaagcc
     3001 attcttttgt acaaagaaca actaaaaatc aaaggtgttg gtttgttagt agcgcgtaga
     3061 gaactgccaa ttcttgaact tgtggcgaaa tcctttgaca aatatgttta agacaagtaa
     3121 aatatctttt gctaactggc ggccgtggtg aagcggttaa cacacctggc tgtgaaccag
     3181 gcattcgggg gttcaagtcc cctcggtcgc cccagtttat
//
LOCUS       DAOSTA010000068         3168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_27585_length_3168_cov_10.958561, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000068 DAOSTA010000000
VERSION     DAOSTA010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27585_length_3168_cov_10.958561"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /gene="dnaB"
                     /locus_tag="PLU08_04920"
     CDS             <1..1519
                     /gene="dnaB"
                     /locus_tag="PLU08_04920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOQ71057.1"
                     /translation="VVMKFDNNPFTLSDKTIAIPNDTILERTVLGLMLTSAEALEVGM
                     QHLEASYFPNIDNYNRAIFYAMKLLHEENVKVDIVTVSGKLRELRLFSAVGGLEYLSL
                     IAEEAITYSAIKDYCLGLRDLDLLRKALKVMDQGVEKHRQGQVGDVNDYLSDLLRDIT
                     KVAEERRIMDFESLGDIAKGLKAHLEKMRKSSGNKLTGNSTGFYDIDRYTHGFQEDNY
                     IIIAARPAVGKTAFGLSLAYNMAKINNKTVGFFSLEMGNQQLMQRLVSKVSGVLHKNI
                     MTGYLSKEERVKVYETIENLKHIKLYIDETPSININDLLIKARKLKRENDDLCAIFVD
                     YIGLITTTGHVESRQIELSEISAALKGLARELNITIVALSQLSRAVEATKDKMPQMSH
                     LRESGSLEQDADLVFLMYREDYYVKQGTIKEIDAVYQPYYQTLAQAKAEMKDPSKDLD
                     VSPVDIILAKNRSGETGIATLLFFKAISSFDNPGPDLTARFTEYRTKKGPSYKHE"
     gene            1516..2274
                     /locus_tag="PLU08_04925"
     CDS             1516..2274
                     /locus_tag="PLU08_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ71058.1"
                     /translation="MKYFVLASGSKGNATIIKGPETKILIDLGLSLKEYKERLNVFKL
                     QEERVFAVLFTHKHGDHITSQFNKVANGNVYAPANTLPFEAPFKLVVPYTPFTINEFK
                     VTPLPTSHDVSDSVGYIIETTEYKLVYMTDTGYISTRNLEMMKNADAYIIESNHSPSL
                     LLKTNRPFDLIQRILSDTGHLSNEACAHYLAELVGPRTKHIVLAHLSEEANTPEVALE
                     TLDKVFRKYGIDQSKLTIVTAKQHVPIMGELNEN"
     gene            2264..2740
                     /gene="rlmH"
                     /locus_tag="PLU08_04930"
     CDS             2264..2740
                     /gene="rlmH"
                     /locus_tag="PLU08_04930"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOQ71059.1"
                     /translation="MKIDVIVVGKTKGALLITSDEYTKMISKYADISIIEIKEERLPL
                     RASEAQIENALNREAETILKRIGERDFVYALSPEGIEFDSIMFSEQLRNAFDIGQSKV
                     MFVIGSSHGLGNEVYKRANLKLSFSKMTFPHQLFRVMLLEQIFRAFKIINNETYHK"
     gene            2755..3132
                     /locus_tag="PLU08_04935"
     CDS             2755..3132
                     /locus_tag="PLU08_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71060.1"
                     /translation="MANNTYNYDNIVPEWNYSEFKHLKRVSNPRTAFARGYLFEEGEF
                     YIEPWFYTQLTRILERFRNEHDEIMDVFFNIARKEKYVLFTRDINEPIFDDENYLLVE
                     IEDIIEGAKLIIDDNSRGSDYGD"
BASE COUNT         1118 a          516 c          606 g          928 t
ORIGIN      
        1 agtagttatg aaatttgata ataatccttt tacactatcg gataaaacaa tagcgattcc
       61 taatgacact attctagaaa gaacagtgtt aggtcttatg ctaacaagtg ctgaagcact
      121 agaagtaggg atgcagcact tagaagcaag ttattttcca aacatcgata attataaccg
      181 cgctattttt tatgcaatga aattattaca tgaagaaaat gtcaaagttg atattgttac
      241 cgtcagcggt aaactaagag aattacgact atttagcgct gttggtggtt tagaatactt
      301 aagtttaatt gccgaagaag cgattacata ttcagcgata aaagattatt gcttaggact
      361 acgtgattta gatttacttc gtaaagcctt aaaagtaatg gatcaaggcg ttgaaaaaca
      421 ccgtcaaggt caagttggtg atgttaatga ttacttaagt gatttattac gcgacattac
      481 taaagttgcg gaagagcgac ggattatgga ctttgaatca cttggtgata tcgctaaagg
      541 gttaaaagct caccttgaaa aaatgcggaa atcttctggt aataaactca caggaaattc
      601 gacgggcttt tatgatatag atcgttacac tcacggtttc caagaagata actatattat
      661 tattgctgca cgtcctgcgg tcggtaaaac ggcatttggt ttaagcttag cctataatat
      721 ggctaaaata aataataaaa ctgttggttt cttctcgctt gaaatgggta accaacaact
      781 aatgcaacgg cttgtttcta aagtttcagg tgtgcttcat aaaaatatta tgacggggta
      841 ccttagtaaa gaagaacgcg taaaagttta tgaaacaatc gaaaacttaa agcatatcaa
      901 actctatatc gatgaaacac caagtataaa tattaatgac ttacttatca aagcacggaa
      961 attaaaacgc gaaaatgatg atttatgcgc catttttgtt gactatattg gtttaattac
     1021 gacaacggga catgttgaaa gtaggcaaat tgaacttagc gaaataagtg cggccctgaa
     1081 aggcttagcg cgcgaattaa atattacaat cgttgcctta tcacaacttt cacgggcagt
     1141 tgaagccaca aaagataaaa tgccacaaat gagtcacctt cgtgaatcag gtagtctaga
     1201 acaagacgca gatttagtgt ttttaatgta tcgcgaagat tattatgtca aacaaggaac
     1261 gattaaagaa atcgatgctg tttatcaacc ctattaccaa acactcgcac aagctaaagc
     1321 ggaaatgaaa gacccgagca aagatcttga tgtttcacca gttgacatta tcttagcgaa
     1381 aaatagaagt ggtgaaacgg gaattgcgac cctgctattt ttcaaggcta tttcaagttt
     1441 tgataatcca ggaccagact taacagcgcg gtttactgaa tatcgcacta aaaaaggacc
     1501 aagttataaa cacgaatgaa gtacttcgtc ctcgctagcg ggtccaaagg taatgccaca
     1561 attatcaaag gacctgaaac aaaaatatta attgatttag ggctcagcct taaagaatat
     1621 aaagaacgac taaatgtctt taaattacaa gaagagcgtg tttttgccgt gctttttact
     1681 cataaacacg gtgatcacat tacttcacaa tttaataaag tcgctaacgg gaatgtgtat
     1741 gctccggcga acactttgcc gtttgaagca ccatttaaac tagttgtgcc ctacacccct
     1801 tttacgatta atgaatttaa agtaacgccc ctaccaacaa gtcatgatgt aagtgattca
     1861 gttggttata ttattgaaac aacagagtac aaacttgtgt atatgactga cactggttat
     1921 ataagcactc gtaacttgga aatgatgaaa aacgctgacg cctacattat tgaatcaaat
     1981 catagtccga gtttattact taaaacaaat agaccctttg atttaattca gcgaatacta
     2041 agcgatactg gtcacttatc aaacgaagct tgtgctcact atttagcaga acttgttggc
     2101 ccgcgcacta aacacatcgt tttagcgcac cttagtgaag aagctaatac tcccgaagtt
     2161 gcgcttgaaa cacttgataa ggtatttcgg aagtacggga ttgaccaaag taaactgaca
     2221 attgttaccg caaaacaaca tgtgccaatc atgggagagt taaatgaaaa ttgatgttat
     2281 tgttgtcggg aaaacaaaag gtgcactttt aattactagt gacgaataca ctaaaatgat
     2341 tagtaaatat gcggatattt caattattga aattaaagaa gaacgacttc cgcttcgcgc
     2401 aagtgaagca cagattgaaa atgcattaaa tagagaagcc gaaaccattt taaaaagaat
     2461 tggtgaacgc gactttgttt atgccttgtc acctgaagga atagagtttg attcaattat
     2521 gttcagcgaa caattacgta acgcctttga tattggtcaa agtaaagtaa tgttcgttat
     2581 cggtagttcc catggtttag gaaatgaagt atataagcgt gcgaacttaa aattaagttt
     2641 tagcaaaatg acgtttcccc atcaactttt tagagtaatg ctccttgaac aaatatttag
     2701 agcattcaaa attataaata acgaaacata tcataaataa ggagaaatat taatatggca
     2761 aataatactt acaattatga caatattgtt ccagagtgga actatagcga atttaaacat
     2821 ttaaagcgtg ttagtaaccc acggaccgct tttgcgagag gatacttatt tgaagagggt
     2881 gaattttata ttgaaccatg gttttataca caattaacga gaatcttaga acgatttaga
     2941 aatgaacatg atgaaataat ggatgtattt tttaatattg ctcgtaaaga aaaatatgta
     3001 ttatttactc gtgatattaa tgaaccaatt ttcgatgacg aaaactatct tttggtggaa
     3061 attgaagata taattgaagg tgcaaaatta attattgatg ataattcacg cgggagcgat
     3121 tatggcgatt aagggcattt ttgtcacgaa tatcagtcat aatagccc
//
LOCUS       DAOSTA010000069         3137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_27971_length_3137_cov_10.073329, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000069 DAOSTA010000000
VERSION     DAOSTA010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27971_length_3137_cov_10.073329"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..267)
                     /locus_tag="PLU08_04940"
     CDS             complement(7..267)
                     /locus_tag="PLU08_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOQ71061.1"
                     /translation="MKGIILYFSKYGATKKYVDWLSKSTNFDVVPVKKAKKSHIKDYD
                     VIILGSGIYASGIKGVKFLRKHAKLLSALKEKVKFTSFLSSL"
     gene            complement(269..1594)
                     /locus_tag="PLU08_04945"
     CDS             complement(269..1594)
                     /locus_tag="PLU08_04945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOQ71062.1"
                     /translation="MNNYDVIIVGAGTAGTYFAYLLGKRGLKVLVIDKDKEKDLAKRL
                     DIFHFTTESFARFNLPESKAGDEEFVSKFSYIYSRSALDNYEKKNFLEVSVMHLPLFI
                     KRLRNKAIKENVEFKFATEFKDLIYDAKGRISGIETKDEKSYNARLVVDASGIPSVVR
                     RVVKDPYMETFPIGPRDKFYVFLKYVKLKDPNIKIDMSLSWPYYKSWIAPQQNKDGAI
                     VGVGANISVDYARKCMKKFEDSIKLPAYDLQYEEWGATPYRRPPYSFVSDGFLVLGDA
                     ACLTQPFNGEGITAAWVQSTPAADVVANALKDDKYPTKEALWPINVMYQTGEGALFAG
                     QRALLVGAVNMSKEDNDYLYANGIIFKSDDEEVKGSVAGNLIKGVLKGKFSFAALRSL
                     MGASSTGNKLTKHYKNYPTSPEKYFAWKKKADRLWKKAGSMADNVKDAD"
     gene            complement(1728..2306)
                     /gene="pduL"
                     /locus_tag="PLU08_04950"
     CDS             complement(1728..2306)
                     /gene="pduL"
                     /locus_tag="PLU08_04950"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOQ71063.1"
                     /translation="MKKILIETSARHIHLSEKDLKILCGEDATLEVRGPLSQPGQFST
                     TTRLTIVGAKRNIERVTVLGPTRKDTQVEVSLTDAFTLGLDVPVRESGDIKGTPGVKL
                     VGPAGEVTLEQGLIVAKRHIHMTPADAKEFNVKNGEVVGVRVKTCDRSLVFEDVVVRV
                     REDFALAMHIDTDEANAAGICGEVYGEIVKLA"
     gene            2449..2982
                     /locus_tag="PLU08_04955"
     CDS             2449..2982
                     /locus_tag="PLU08_04955"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOQ71064.1"
                     /translation="MLKKFLSNKQNQAEILRFIIVGVICTLVDFGVSSLIQFVIYPVA
                     EALKIGPFTITPNIFLAALFGFIFGVITNYILSVIVVFKNVENKKTSRSAKGFIIFVL
                     LSTGGFLINYAIKELGNLIIPMDTNYIWFVFIFGVATFVVLIYNYVTRKLILFKPKKE
                     EMIKSDENTTTSSQEKE"
     gene            3046..3129
                     /locus_tag="PLU08_04960"
     tRNA            3046..3129
                     /locus_tag="PLU08_04960"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3080..3082,aa:Leu,seq:gag)
BASE COUNT          936 a          615 c          512 g         1074 t
ORIGIN      
        1 taaacattat aatgaagaca agaaggatgt aaatttaacc ttttccttta acgcgcttaa
       61 taatttagcg tgtttacgca gaaatttaac acctttaatt cctgaagcat atataccgct
      121 accaagaata attacatcat aatctttgat atggctcttt ttagcttttt taactgggac
      181 aacatcaaag ttagtactct tgcttagcca atcgacatat tttttggtcg ctccgtattt
      241 agaaaagtag agaattattc ctttcaactt aatcagcatc ctttacatta tccgccattg
      301 aacctgcttt tttccacaag cgatcagctt ttttcttcca cgcgaaatat ttttctggtg
      361 aagtaggata gtttttataa tgttttgtta atttgttgcc cgtgctacta gcgcccatta
      421 aactacgaag tgccgcaaac gagaactttc cttttaagac acccttaatt aagttacccg
      481 ctacacttcc ttttacttct tcgtcatcac tcttaaagat aatgccgttt gcgtataagt
      541 aatcattatc ttctttactc atattaacag ccccgacaag aagcgcgcgt tgaccagcaa
      601 agagtgcacc ttctcccgtt tgatacataa cattaattgg ccataatgct tcttttgttg
      661 gatatttatc atcttttaag gcgttggcga ctacatctgc ggctggcgtc gattgcaccc
      721 aagcagcggt aatgccttca ccgttaaaag gttgcgttaa gcatgctgca tctcctaaga
      781 caagaaaacc gtcacttaca aaactatagg gcggacgccg atatggggtt gcgccccact
      841 cttcatattg caaatcatat gcgggtagtt taatactatc ttcaaacttc ttcatacatt
      901 tccgcgcata atcaacggat atattcgcgc caaccccgac aattgctccg tctttatttt
      961 gttgtggggc gatccagctt ttgtaataag gccaacttaa agacatatca atcttaatgt
     1021 tcggatcttt tagttttaca tattttaaga acacatagaa cttgtcgcgt ggaccaattg
     1081 gaaatgtttc catgtatgga tcttttacta ctcttcttac gacactaggg attcctgaag
     1141 catcaacaac aagccgagcg ttataacttt tttcatcctt tgtttctatc ccgcttattc
     1201 gtcctttagc gtcataaatt aaatctttaa actctgtagc aaacttaaat tcaacattct
     1261 ctttgattgc cttattgcgt agtcttttaa taaataaagg caaatgcatg acgcttactt
     1321 ctaagaaatt tttcttttca taattatcaa gcgctgaacg cgaataaata taagaaaact
     1381 ttgagacaaa ctcttcatca cctgcttttg actccggtaa attaaagcgg gcaaaagatt
     1441 ctgtagtaaa gtggaaaatg tctaagcgtt tcgctaaatc tttttcttta tctttgtcga
     1501 ttacaagtac ttttagcccg cgtttcccta gtaaataagc aaaataagtg ccagcggtac
     1561 ctgccccgac aataattaca tcataattat tcatatgtat ccctccattt ttattgtgac
     1621 atatttatta ctaaaagtaa ttagtgttta cgataatttt aaaatttata aacaaaaaaa
     1681 catctcgtga agtagatgtt taatgtttta ataataatta gattaatcta ggcaagtttt
     1741 acaatttcgc cataaacttc gccacagata cccgctgcgt tagcttcgtc agtgtcaatg
     1801 tgcatagcaa gcgcaaagtc ttcacgaacc cgcacgacaa cgtcttcaaa cacaagacta
     1861 cgatcacatg ttttaacacg aacgccgacg acttcaccat ttttaacatt gaactctttt
     1921 gcgtcagctg gtgtcatatg gatatgacgt tttgccacga ttaaaccttg ttctaaagta
     1981 acttcacctg ctgggccaac aagcttaaca cctggggtgc ctttaatgtc accactttca
     2041 cgcactggga catctagtcc taaagtaaaa gcatcagtta gtgaaacttc aacttgcgta
     2101 tctttacgcg ttggtcctaa cacagtaacg cgttcaatat tacgtttcgc accaacgatt
     2161 gttaaacgtg ttgtcgtgga aaattgcccg ggttgactaa gcggtcctct aacttctaaa
     2221 gtagcgtctt caccacataa tatttttaag tctttttcac ttaaatgaat gtgacgtgca
     2281 gatgtttcaa taagtatttt tttcatgtaa ctttctcctt tatatagcac ataaattata
     2341 acaataatca tcttattttg ctattgactc actattaata gtgaaattga aggcggattt
     2401 agttaaaaat gataatatat tatttaccga gaaaggaaat tcttagttat gttaaagaag
     2461 tttttatcaa ataaacaaaa ccaagctgaa atattacgct tcattattgt tggtgtaatt
     2521 tgtactttag tggattttgg tgttagttca ctgattcaat ttgttattta ccccgtcgct
     2581 gaagcactga agattggacc ctttacaatt acaccaaata tatttctcgc agcgcttttt
     2641 ggttttatct ttggggtaat cactaactac attctttccg ttattgttgt ttttaaaaat
     2701 gtcgaaaata agaaaacatc ccgttcagcg aaaggtttta ttatttttgt gctgttatcc
     2761 actggtggtt ttttaataaa ttatgccatt aaagaattag gtaacttaat tattccgatg
     2821 gacactaatt atatttggtt tgtattcatc tttggtgttg cgacattcgt tgtcttaatt
     2881 tataactatg tgacgcgtaa acttatttta tttaaaccta aaaaagaaga aatgataaaa
     2941 agtgatgaaa acaccactac ttctagtcaa gaaaaagagt aaataacaat cttttgtatt
     3001 attgtgattg aaacatttat aaaagttgtt ataatttaat tcgacgcagc catggcggaa
     3061 ctggtagacg cgtacgtttg aggggcgtat ggtaatcccg tgtgagttca agtctcactg
     3121 gctgcaccag ttatcaa
//
LOCUS       DAOSTA010000070         2971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_30080_length_2971_cov_11.613169, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000070 DAOSTA010000000
VERSION     DAOSTA010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30080_length_2971_cov_11.613169"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..959)
                     /locus_tag="PLU08_04965"
     CDS             complement(171..959)
                     /locus_tag="PLU08_04965"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOQ71065.1"
                     /translation="MYRPIKRTLDFVLSLLAIIILSPLLLFIAILVRLKLGGPVLFKQ
                     KRVGRYNTIFNLYKFRSMKNAYDEKGELLPDEQRLTKFGIFLRRTSLDELPQLFNILF
                     GKMTIIGPRPKTIFETLLMKDTKFVYRAAIKPGLSSWSVIHGRNNMPNDKALMYDFEH
                     VIKFSAWLDTKIFFKTIGLVLRGSGINTDGHATFLHLSDFLIEKKIKTPEEIEKVRQE
                     AIIIEKENMKVVPMGIIKEDFKAKRREIQKNRKSYTPNQVITWD"
     gene            complement(1018..2826)
                     /locus_tag="PLU08_04970"
     CDS             complement(1018..2826)
                     /locus_tag="PLU08_04970"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOQ71066.1"
                     /translation="MKAFIFNSGVGSRLGDLTKDKPKALVELKSGETILGRQLRILRS
                     QGIKEIVISTGYKEEMIKEYCLKEFPELHFTFVFNPDYATTNSIYSMYLAENKFQDDF
                     LIMHGDLVFDEGIVEKLLEEKHRDLVCINTEIPKPPKDFKGRIKKEKLQKIAVDIFAK
                     DDYALQPLYKLSFATISQWMLRIKEMIGKGIVDVYAENALNEALLDLDVRCVDYKYNF
                     VEEIDTLEDYERVSNDVRLFEFLNQKVYHGDYLIKLIEIIKKHNFKRPFLVYSSATFK
                     NTEFYNYMKNLALTPYNSYSSNPKYEEIIPGLELFKRNKCDVIVAIGGGSAIDVAKAI
                     KLFSGLNPNINYLEQKLVYVNTPLIAIPTTAGTGSETTRYSVIYVDGEKYSLTHDCIF
                     PNIAVIDPTFLRTLPEYHRRSSLLDALCQAIESFWSKGSIEETRLLSARGIHLFRYTY
                     KHYLEGHPFVDDRIMKVSYLAGQAINITATTAPHAMSYKLTSLTGIAHGHAVSVTLPY
                     VYKYMLEIAKKSEDKELKQTFVNLAKIFETSETKLFEVILNIFNEFELEKPTVTEDQL
                     IELINDVNEERLQNNPVLLDKEAIEEIYRSALIVKK"
BASE COUNT          967 a          518 c          396 g         1090 t
ORIGIN      
        1 aagaagtaat atcatcaagc actaatatta caaagtaatc ttttaataat ttatccggca
       61 taaaagtcct cctaaaaagt tggattcata gacattatac tataaagtaa aagaatatta
      121 tatttttaag ggtcttcttt atttttataa aataaaaagt gttactaaca ctagtcccaa
      181 gttatcactt ggttaggagt atatgattta cgattttttt gtatttcgcg acgtttagcc
      241 ttgaagtctt ctttaataat tcccatcggt acaaccttca tattttcttt ttcaataata
      301 atcgcctctt gtctaacttt ttcaatttct tcaggggttt taatcttctt ttcaattaga
      361 aaatcactta aatgtaaaaa tgtagcgtgc ccatcagtgt taatccctga accgcgtaac
      421 actagaccga ttgtcttaaa aaatatttta gtatctaacc atgctgaaaa tttaataaca
      481 tgttcaaagt cgtacatcaa tgctttatcg ttaggcatat tattacggcc gtgaattaca
      541 gaccaactac taagtcctgg tttaattgcc gcacgataca caaacttcgt atctttcatt
      601 aatagcgttt caaaaatagt tttaggacgg ggaccaataa ttgtcatttt tccaaacaaa
      661 atattaaaaa gttgtggtaa ttcgtctaga cttgtacgtc ttaaaaatat cccaaacttt
      721 gttaatcttt gttcgtctgg caacaattca cctttttcat cataagcgtt cttcattgac
      781 ctaaatttat ataaattaaa tattgtatta taacgaccca ctcttttttg tttaaagaga
      841 acagggccac ctaattttaa acgcactagg atagcaatga ataataaaag cgggctcaat
      901 ataataattg caagtagcga taatacgaag tctaatgttc ttttaattgg tctatacata
      961 attaattacc atgcacacat agtgtttaca ttttaccata tgtaatcatc acatgtctta
     1021 tttcttaact ataagtgcac tcctataaat ttcttcaatt gcttctttat ctaacaaaac
     1081 ggggttattt tgtaatcttt cttcgtttac atcattaatc aattcaataa gttgatcttc
     1141 agttactgtt ggtttttcta attcaaattc attaaaaatg tttagtataa cttcaaaaag
     1201 ttttgtctcg ctagtttcaa aaattttagc aagattgaca aatgtttgtt taagttcctt
     1261 gtcttcgctt tttttagcaa tttctaacat atatttataa acatacggta atgtaacact
     1321 tacagcatga ccatgggcaa taccagttaa tgatgttaat ttatagctca ttgcatgtgg
     1381 agcggtagtc gcagtaatat taattgcttg gcccgctaag tatgaaactt tcataatacg
     1441 atcgtctaca aaagggtgac cttctaaata atgtttataa gtatacctaa ataaatggat
     1501 tccccttgca cttaatagcc tagtttcctc tatagaacct tttgaccaaa aactctcaat
     1561 cgcttgacat aatgcatcta ataatgagga gcgacggtga tattctggta atgtgcgtaa
     1621 gaatgtcggg tcaataacag cgatatttgg aaaaatacaa tcgtgtgtta atgaatattt
     1681 ctcaccatca acataaataa ccgaatacct tgttgtttca cttccagttc ctgctgttgt
     1741 aggaatagca ataagtggag tattaacata aactagtttt tgctctaaat aatttatatt
     1801 cggattaaga cctgaaaata acttaattgc tttggcaaca tcaatggcag aaccaccccc
     1861 aatcgcaaca attacatcac atttatttct tttaaataat tctaatcccg gaattatttc
     1921 ttcatattta gggtttgaag aataactatt gtatggagtt aaagcaagat ttttcatgta
     1981 attataaaat tcagtgtttt taaaagtagc gctgctataa acgagaaatg gtcgtttaaa
     2041 gttatgtttt tttattattt ctataagttt aatcaaataa tcgccgtgat atactttttg
     2101 attcaaaaat tcaaaaagcc taacatcatt actaactctt tcgtaatctt ctaaagtatc
     2161 aatttcttcc acgaaattat acttataatc aacgcatcgc acatctaaat caagtagtgc
     2221 ttcatttaaa gcattttctg cataaacatc aacaataccc ttaccaatca tttccttaat
     2281 acgaagcatc cattgactaa ttgtggcaaa acttagttta tataatggtt gcaatgcata
     2341 atcatctttt gcgaagatgt ctacagcaat tttttgaagt ttttctttct taattctgcc
     2401 cttaaagtct tttggtggtt ttgggatttc agtatttata cagactaaat cgcgatgttt
     2461 ttcttccaat agtttttcca ctattccttc atcaaacact aaatcaccat gcataatcaa
     2521 aaaatcatct tggaatttat tttccgctaa atacattgaa tagatgctat tagtagtagc
     2581 ataatcaggg ttaaatacaa aagtaaaatg taactctgga aactctttta aacaatattc
     2641 tttaatcatt tcttctttgt aaccagttga aataactatc tctttaatac cttgacttct
     2701 taaaatacga agttgtcgtc ctaaaatggt ttcaccactt tttaattcca ctagtgcttt
     2761 tggtttatct tttgttaaat cacccaaacg tgatccaaca ccactattaa atataaatgc
     2821 tttcatatta tttacgactt cttcttaaga atttcattaa taagtctttg ttttcaactg
     2881 gggtagttgt gggccgccct aaatcatcac gacttccttg attaacatac actactaatg
     2941 cagctttatt tttccgttta aactttggaa a
//
LOCUS       DAOSTA010000071         2877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_31398_length_2877_cov_9.915308, whole genome shotgun sequence.
ACCESSION   DAOSTA010000071 DAOSTA010000000
VERSION     DAOSTA010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31398_length_2877_cov_9.915308"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..528
                     /locus_tag="PLU08_04975"
     CDS             22..528
                     /locus_tag="PLU08_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71067.1"
                     /translation="METKNINTKQLSIIVRIGIIFLFIIGLIMVIVWYPFTISLTIIG
                     PVDVEPTQLQKIQVYAQSAFYYLTSVPCFIILIFLWIIAGRLKKNGRFIAKDVRLLNA
                     ANIMLLIDLAIFLVGNIIIVFLEINFFVLAHFFIAVIGLVVLIFMWTTTLLIRRNVEL
                     QALIETDS"
     gene            672..992
                     /locus_tag="PLU08_04980"
     CDS             672..992
                     /locus_tag="PLU08_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008402371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOQ71068.1"
                     /translation="MDVQLKRGLLEVCVLAVIRNKPTYGYRIIKDMKPYVEISESTLY
                     TILKRLEENEMLTVKTVEHNSRLRKYYSITGRGLGRIAEFKEYWKDMEAMYKFIIKKE
                     SSDD"
     gene            1297..1587
                     /locus_tag="PLU08_04985"
     CDS             1297..1587
                     /locus_tag="PLU08_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71069.1"
                     /translation="MLIGAIAVFFSLVISLWAVDLGLFVGSFEAFITVFTLLFAGEGL
                     KALAYFGISLAMAGLGILLLLVGVKLTKYTIELAKKLINKIKTKMVLKGAKK"
     gene            1584..2549
                     /locus_tag="PLU08_04990"
     CDS             1584..2549
                     /locus_tag="PLU08_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOQ71070.1"
                     /translation="MNKLEKILTIVASSLLVVGLVIFISVLAVNGFNLGGLSTVKYEA
                     VTHEVSEDYAKIQFDISVGDVTIVKGEEATTKAICYENKRLTYNLNVEEDTLVVTDNN
                     TMRRFWTIFDLWGNKGITLYVPHDTYTSLLVKNNTGTLNVSSSVTFNTITARLETGSI
                     TLNGDASVVDLSSETGSITLNNVDADSARLVTATGRHVLNNVNVTGNLNIEVDTGTVK
                     LTNVKSDNLTVDSDTGTITLKDTVVTNTMRLAASTGSINFDKSDAHDIYARTSTGSIK
                     GSLLTGKVFSTKTNTGTISVPSNTAGAGVCDLRTDTGVIKITIAA"
     gene            2658..>2877
                     /locus_tag="PLU08_04995"
     CDS             2658..>2877
                     /locus_tag="PLU08_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71071.1"
                     /translation="MKQSTNKVEFILDEYEYFEDLYIENGASDVRLVKTKRPNVRVVA
                     VNLSKYRVFVEKSILFVKEEHEYKYRAPF"
BASE COUNT          920 a          406 c          594 g          957 t
ORIGIN      
        1 ttttagcgtt ataattatgt tatggaaact aaaaacataa atacgaaaca attatcgatc
       61 attgtccgca ttggtattat ttttcttttt atcatcggct taattatggt tattgtctgg
      121 tatcctttta ctatttcgct tacaattatc ggcccagttg atgttgaacc aacacagtta
      181 caaaagattc aagtatatgc gcaatcagcg ttttattatc ttacatcagt gccatgtttt
      241 atcattttaa tatttttgtg gataatcgct ggtcgcttaa agaaaaatgg tcggtttatc
      301 gctaaagatg tgcgtttgct taacgctgct aatattatgt tattaattga tttagcgata
      361 tttttagttg gtaatattat tattgttttc ttagaaatta atttctttgt acttgcgcat
      421 ttctttattg ctgtaattgg gcttgtcgta ttaattttta tgtggacaac aacattactt
      481 attaggcgta atgttgaatt acaagcactg attgaaactg attcttaata atgtccttaa
      541 tcatttttaa atttagtagt ttataactac tcttttttca atttaaaaga aaatgtaaat
      601 ttttaataat atgaattgac tttttatata cttcggctta cgatgtatta tgttaaggga
      661 ggtataagac tttggatgta caactaaaac gaggactctt agaagtttgt gtacttgctg
      721 ttattcgtaa taaaccaact tatggctacc gtattattaa agatatgaag ccatatgttg
      781 aaatatctga atcgacatta tacacaatat taaagcgtct cgaagaaaat gagatgttaa
      841 ctgttaagac agttgaacac aattctcgcc tccggaaata ttacagtatt acagggagag
      901 ggcttggccg cattgctgaa tttaaagaat actggaaaga catggaagcg atgtacaaat
      961 ttattattaa gaaggagagt agtgatgact aaaaaggagt ttctaagtgc cttgcgtgaa
     1021 aagatggcag gattaccaaa agacgatgtg gacgatcgta ttgcttttta tagcgaaatg
     1081 attgatgacc gcatgtaaga aggactaagt gaagcagaag cggtcgccga agttggtaat
     1141 gttgatgaga tcgtcattaa acaaattgaa ggcacatcaa ttactaagat tgttaaagac
     1201 aaagtaacac caaaacgaaa gttagaagga tgggaaattg ttctaattat tgtacttgca
     1261 ccgctttggg taccgcttct tattggatta ggcggtttgc ttatcggggc aattgcggtg
     1321 tttttctcgc ttgtaatttc gctctgggcc gtagaccttg gtctttttgt cggctcattt
     1381 gaagcgttta ttactgtctt tactttgctt tttgctggtg aaggacttaa agcactagcc
     1441 tatttcggga taagtcttgc aatggcggga ctagggatcc ttttattact agtgggtgtt
     1501 aagttaacga agtacacaat tgagttagcg aaaaagctta ttaacaaaat taaaacaaag
     1561 atggtgttga agggagcaaa aaaatgaata agttagaaaa gattttaacg atagttgcga
     1621 gttccttgct cgtagttggc ctagtcatat ttattagtgt tttagcggta aacggcttta
     1681 atttaggagg attaagtacg gtgaaatatg aagctgtaac tcatgaagtt agtgaagatt
     1741 atgcaaagat tcaatttgat atcagtgttg gggatgtcac gattgttaaa ggtgaagaag
     1801 caacgactaa agcgatttgt tatgaaaata aacgcttaac ttataacctt aacgttgaag
     1861 aagacacgct tgttgttact gacaataata cgatgcgtcg tttctggact atctttgatt
     1921 tatggggtaa taaaggtatt acgctttatg ttccgcatga tacttatact tcacttttag
     1981 tgaaaaataa tacggggaca ttaaatgttt caagcagcgt aacttttaac acgattacgg
     2041 cacgattaga aacggggtcg atcacactaa atggtgatgc aagtgtcgta gatcttagct
     2101 cggaaacagg ctcaatcact ttaaataatg ttgacgcaga tagcgctaga ttagtgacgg
     2161 caactggaag acacgtctta aataatgtta atgtaacagg aaacctaaat attgaagttg
     2221 atactggcac agttaagtta acgaatgtta aaagtgataa tttaacagtt gatagtgata
     2281 ctggtacgat tactttaaaa gatacagttg tgacaaatac aatgcgttta gccgctagta
     2341 cgggctccat taactttgat aaaagtgatg cccatgacat ttacgctaga acttcaacag
     2401 gtagtattaa aggttcactc ttaactggta aagtctttag cactaagaca aatactggta
     2461 ccattagtgt gccaagtaat accgctggtg ccggagtttg tgatcttcgc actgacactg
     2521 gagtgattaa gattacgatt gcggcatagt taaattttgt cccccgagtg agtgcgttag
     2581 aagtaacgca ctttttctta tttaaaagta ttgttacatt aagcgtgata aaattagaaa
     2641 aataggagtc tcttaaaatg aaacaaagta ctaacaaagt agaatttatt ttggatgaat
     2701 atgaatattt tgaagatctg tatattgaaa acggcgctag tgatgtgcgg cttgtaaaaa
     2761 caaagcgacc aaatgtgcgt gtagtagcgg ttaatttatc aaaatatcgt gtttttgtcg
     2821 agaaatcaat cctttttgtt aaagaagagc atgaatataa atatagagct ccttttg
//
LOCUS       DAOSTA010000072         2677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_34601_length_2677_cov_7.033181, whole genome shotgun sequence.
ACCESSION   DAOSTA010000072 DAOSTA010000000
VERSION     DAOSTA010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34601_length_2677_cov_7.033181"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    complement(74..275)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(375..2591)
                     /locus_tag="PLU08_05000"
     CDS             complement(375..2591)
                     /locus_tag="PLU08_05000"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOQ71072.1"
                     /translation="MEKKSHNLPMPKVHSWQDVKDVYFLYITNAEDRANIEEAYNFAL
                     KKHEGQFRKSGHPYITHLIEVAFILAELTAGPSTIIAGLLHDVVEDTDVTVSEIDKMF
                     GKDVAMIVDSVTKIQRLKLSKKVDDSDFVYEDHRKIFLGMAKDIRVIIIKLADRLHNL
                     RTLYFLSHERQLRIAKETMAVYAPIAGRLGMGKIKAEMEDLSLKYIEPEAFNHVSELV
                     EQRSPNLQKALAEVSKRIADILFKNDIKFEMKFRVKSIYSIYKKMKEKDNDFERIYDL
                     LALRIITPTELNCYEILGLIHATYRPVTGRFKDYIAMPKSNMYQSLHTTIFTGDGNAL
                     EVQIRTPEMDRIADSGVAAHWAYKEGTNYNPEREQREIENKLHWLRDFVATGYETGNT
                     AKEYMDTITKEIFETSVYVFTPKGNVIELPAGATPLDFAYKIHTKVGDTAVGALVNDK
                     LVAMNTPLKTGDICEIRTSKTATGPTESWLNIATTNSARSHIRKVLAKKNQALTRDDN
                     IEKGRGSLNDAFKERGFAEHEVEPHVNKPEVLKHFNVNTYEDLLLLVYSRNITASAVL
                     DYLKLDRRDHMGKLLSKGRRVVEDAKNPVIVPGAGTVAVTLGNCCTPVPGDNIVGYIT
                     KGKGITVHRINCPNIARNLDRTIEVLWNPNSETKLYPLDISIKCNDRDNLLVDIMNAL
                     SQAKVSISKIKATYHPSTQTSVIDATILIPNVDKLNSVQNIILQVDSVYKIDRVIH"
BASE COUNT          764 a          539 c          439 g          935 t
ORIGIN      
        1 gaatcttttt gatatatatt tactatttaa cttgattaag aatagttaat ctcctctaaa
       61 taaaaaaagg tagtataagg acgggattgc cgcggtacca ccttatttcg tttgttaatt
      121 aaacgcatct ctaactcgta acgtgagtaa cggtcctgct tatttattac aagacttgtc
      181 tccatggtgt cctttaacaa tacttcgcta gttttgcacc aaccaactag tctctattcc
      241 ggaatatttg ttaaaatcca tatcaatgac tttttaatta taaaataatt aactttaata
      301 gtcaatgtaa gcggcgacta ttattcacta attatctact attaaactac taataaaata
      361 cttttagcgt tattttaatg aattacgcgg tcaattttat aaactgagtc aacttgcaag
      421 ataatatttt gcacactatt taatttatca acattcggaa ttaaaatcgt tgcatcaatc
      481 acacttgttt gggtcgatgg gtgataagta gccttaattt ttgagatact aacctttgct
      541 tgacttaaag cattcataat atcgactaat aagttatcac ggtcattaca cttgatacta
      601 atatctaacg gatataattt agtttcgcta tttggattcc atagtacttc aatcgtgcgg
      661 tcaaggttac gcgcgatatt tggacaatta atgcggtgaa cggtaatccc tttccctttc
      721 gtgatataac caacgatatt atcacctggc actggtgtac aacaattacc aagtgtgacc
      781 gcaacagtgc ccgcccctgg gacgattacg ggatttttag cgtcttctac tactcttcgt
      841 cctttactta aaagtttgcc catatggtca cggcgatcaa gttttaagta atctaatact
      901 gcactagcgg taatattccg tgaataaaca agtaatagta agtcttcata agtattaaca
      961 ttaaaatgtt ttaagacttc tggtttattc acatgtggtt caacttcatg ttcggcaaag
     1021 ccgcgttctt taaaggcgtc atttagtgag ccacgccctt tttcgatatt atcatcgcgc
     1081 gttaaagctt gatttttctt cgctagcact ttacgaatat gactgcgtgc actattcgtg
     1141 gtggcaatat taagccatga ctctgttggt cctgtagccg ttttactcgt tctaatttca
     1201 caaatatcac cggttttaag tggcgtattc attgctacaa gcttatcatt aacaagtgcc
     1261 ccaaccgctg tatctccgac tttagtgtga attttatagg caaagtcaag tggtgttgct
     1321 cccgctggga gttcaataac attacctttt ggcgtaaaga catacacact agtttcaaag
     1381 atttcttttg taatcgtgtc catgtattct ttcgcggtat taccagtttc ataacccgtt
     1441 gccacaaaat cacggagcca atgtaattta ttttcaatct cacgttgttc gcgttctgga
     1501 ttataatttg taccttcttt atatgcccag tgcgctgcaa cccctgagtc agcgatacgg
     1561 tccatctctg gcgttcttat ttgcacttct aaagcattac cgtcccctgt aaagatagtt
     1621 gtgtgtaaac tttgatacat attgcttttt ggcatcgcga tataatcttt aaagcgtccc
     1681 gtaactgggc gataagtcgc gtggattagt cctaaaattt cataacaatt aagttccgtt
     1741 ggagtaatga ttctaagcgc taaaaggtca taaatccgtt caaagtcatt atctttctct
     1801 ttcatcttct tataaatact ataaatcgac ttcacacgaa acttcatttc aaatttaatg
     1861 tcatttttaa ataagatatc cgcaattcgt ttactaactt ccgcaagcgc cttctgtaag
     1921 tttggggagc gctgctcaac gagttcactt acatgattaa aggcttctgg ttcaatgtat
     1981 tttagactta agtcttccat ttctgcttta attttcccca tccctaggcg tccagcaatc
     2041 ggagcgtaaa ccgccatcgt ttctttagca atacgcagtt gtcgttcatg gcttaaaaag
     2101 tataatgttc gcagattatg taatctgtcc gcaagtttaa taatgataac acggatgtct
     2161 tttgccatcc ctaagaatat tttacggtga tcttcataca caaaatcact atcatcaact
     2221 tttttactaa gttttaggcg ttgaatttta gtgacgctat cgacaatcat cgccacatct
     2281 ttgccaaaca ttttatcaat ctcactaact gttacatcag tgtcttcaac gacatcatgc
     2341 agtaaaccag caataatcgt acttggaccc gctgttagtt cagcaagaat aaaggcaacc
     2401 tcaattaaat gcgtaatata aggatgaccg cttttacgaa attgcccttc atgtttttta
     2461 agcgcaaaat tatatgcttc ttcaatatta gcgcggtctt ctgcattagt aatatagaga
     2521 aaatagacat ctttgacgtc ttgccacgaa tggacttttg gcattggtag gttatgactt
     2581 tttttctcca tactatgcgt ttcctttctt attaattata acaaattaat aatcgctttg
     2641 attatgcttt acttaataag ctgcttttaa taaaaaa
//
LOCUS       DAOSTA010000073         2526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_37436_length_2526_cov_9.447592, whole genome shotgun sequence.
ACCESSION   DAOSTA010000073 DAOSTA010000000
VERSION     DAOSTA010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37436_length_2526_cov_9.447592"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..373)
                     /locus_tag="PLU08_05005"
     CDS             complement(77..373)
                     /locus_tag="PLU08_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ71073.1"
                     /translation="MYCEKLITLRRNRGLTQQQLADALMLSQSMISKIERGITRLDPM
                     LAIRFADFYNVSLDYLFGRSERQLQISEKDIENLSEQDKNEMLAFIIKLINEKK"
     gene            complement(543..2234)
                     /locus_tag="PLU08_05010"
     CDS             complement(543..2234)
                     /locus_tag="PLU08_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ71074.1"
                     /translation="MNNKVPLSEYPRMIMVRDSYLSLNGVWEYAIRENDERVPIEFDG
                     DILVPFSPETPLSGVNRTVRPNDVLFYRKRFSLPKGFKKEYVTLHFGAVDQVVTVYLN
                     GTKIGFNQSGFHPFSIEISKVLKIDNELVLVVRDYSDTRFYARGKQKIKRGQIWYTPQ
                     SGIFMPVWLESMPRDYIEKLKITPRYDESLVEITVFTNSKDNDAVIIFDGKEYTVKPN
                     KVYKLKVKDFISWSPDNPHLYPFKVKYCEDEVDSYFAMRKISVDTDEEGIKRIYLNNK
                     ILFNTGLLDQGYYHEGYLTPPDEATVINEIKVAKEMGFNVLRKHIKIEWERFYYHCDR
                     LGMLVWQDFVNGGEPYNFVTVHVPAVLNIHFKDTNYKRFARQNALGRELFYAEAKTTI
                     EHLYNYPSIVLWTIFNEGWGQFDSHKLLPFVLELDNTRLIDVNSGWHDMGLGQFRSHH
                     VYFRKYRFKKDKLDRCVILSEFGGYAHRIPGHYYGVANFGYRKYNTKEELEKAVINLY
                     EKEIIPAVKQGLAAAIYTQLSDVEDELNGVLTYDRKVVKIDPKVMRELNEKLLKA"
BASE COUNT          812 a          463 c          378 g          873 t
ORIGIN      
        1 ttaattatta tttttatccc ctaaactttt ctagtgtata accctaaaga cgggtatcgc
       61 gattaatata tcttcattat ttcttttcgt taataagttt aataataaac gctaacatct
      121 catttttatc ttgttctgat aaattttcaa tatctttttc gctaatttgt aactgccttt
      181 cactacggcc aaacaagtaa tcaagtgaaa cattataaaa atctgcaaaa cgaattgcta
      241 gcattggatc aagccgagtt atgccgcgtt ctattttaga aatcattgat tgcgacaaca
      301 ttaaagcatc ggcaagttgc tgctgggtta aaccacgatt acgacgtaaa gtaattaatt
      361 tttcgcaata catgatttta tcttaactaa actaaaacgc ttttacttta ttaattgctt
      421 aatattctaa tttagaataa aatggcttta caaatattct gtaatggaat attaaaaaga
      481 cattatcaat gcataattgg caggaaccga atcgcaaaga taatgtcttt tctttaaatg
      541 ggttaagctt ttaagagttt ttcgttaagt tcacgcataa cttttggatc aatcttaaca
      601 acctttcggt cataagttaa tacaccattt aattcgtctt cgacatcact taattgggta
      661 tatatcgccg ctgctaggcc ttgcttaact gcaggaataa tttctttttc atacaggtta
      721 attaccgctt tttctaattc ttctttagtg ttatatttgc gatatccaaa gttagcgacc
      781 ccgtaatagt gtccaggtat ccgatgcgca taaccgccaa attcacttag gataacgcag
      841 cgatcaagct tatctttttt aaagcgatat ttacgaaaat aaacatgatg actgcggaac
      901 tgtccaagcc ccatatcatg ccaacctgaa ttaacatcta ttagtcgagt attatcaagt
      961 tcaagcacaa acgggagtaa tttatgacta tcaaattggc cccagccttc attaaatatt
     1021 gtccataaaa caatggacgg atagttatat aaatgttcaa tcgttgtttt agcttcagcg
     1081 tagaataatt cgcgtccaag ggcattttgc cgtgcaaaac gcttataatt tgtatcttta
     1141 aagtgaatat ttaacactgc tggaacatgt acagtaacga agttatacgg ttcgccacca
     1201 ttaacaaaat cttgccacac tagcatccct aagcgatcac aatgataata aaagcgttcc
     1261 cattcaattt tgatatgttt ccgtaaaaca ttaaagccca tctcttttgc gactttaatt
     1321 tcattaataa ctgttgcttc atcgggtggt gttaaataac cttcgtgata atagccttga
     1381 tcgagtaatc ccgtattaaa aagtatctta ttatttaaat atatgcgttt aatcccttct
     1441 tcatctgtat caacactaat tttacgcatc gcaaagtacg aatcaacttc atcttcacag
     1501 tatttcactt taaagggata gagatgcggg ttatcaggtg accaactaat aaagtcttta
     1561 accttaagct tatatacttt gttaggtttt acagtatatt cttttccatc aaaaataatt
     1621 actgcatcat tatcttttga attagtgaaa acagttatct ccacaagtga ctcgtcatag
     1681 cgcggagtaa tctttaattt ttcaatataa tcacgcggca tactttcaag ccaaactggc
     1741 ataaaaatac cgctctgtgg ggtataccat atttgtccgc gtttaatctt ttgtttacca
     1801 cgggcataaa acctagtgtc actataatca cggacaacga gcactagttc attgtctatt
     1861 tttaggactt tactaatttc aatagaaaac ggatggaaac cactttgatt aaagccaatt
     1921 ttagtaccat ttaaatacac agttacaact tgatcaactg cgccaaaatg gagcgttaca
     1981 tattcttttt taaaaccctt tggtaacgaa aagcgtttac gataaaaaag aacatcattt
     2041 ggtcttactg tgcggttaac accagaaagc ggtgtttctg gtgaaaaagg cactaaaata
     2101 tcaccatcaa actcgattgg tactctttca tcattttccc taatagcgta ttcccatacg
     2161 ccgtttagtg ataagtaaga atcacgaacc ataatcattc gtgggtattc acttaatggt
     2221 actttattat tcatattcat tgctcctttt catcttgaaa taaatattta ttgtttttat
     2281 tattgcctta attagtttct aaaggtttgt tcatatatca tctttattat acaagattag
     2341 cgccatctta agaggcaata cataatgtta actatcattt attactatgt aataagcgaa
     2401 attctaaagt atttttgcaa aagtaattca tagaatcaaa acatcgtatt gaaatacttg
     2461 caaaaaagtt ggaactttat tatagtatat aggctggacc agtagtgtag tggttaacat
     2521 gtctgc
//
LOCUS       DAOSTA010000074         2128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_47417_length_2128_cov_10.112880, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000074 DAOSTA010000000
VERSION     DAOSTA010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47417_length_2128_cov_10.112880"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PLU08_05015"
     CDS             <1..869
                     /locus_tag="PLU08_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HOQ71075.1"
                     /translation="AIMAIKGIFVTNISHNSPKFRIQVDEFLNKAKLIPNLELIEVKN
                     QHVLSYLNNNTPDFCLFWDKDIYLAKRIENSGIKVFNNPETIYLCDDKALTAAELAKA
                     RVAQPRFFVFPLHYWGNIIEYYEEYKEELLALSFPLVIKERFGSFGDQVYLAHSEEEL
                     KKIIKEHGTKGLLAQEFVSGEKGTDYRVNVVGDKVIFTVKRVNDKDFRSNINQGGTAS
                     LVKATSEIEDLAIQATKALKGHFAGVDIMLDAKGKPVVIEVNSNMRTVAVNKVSDIDL
                     TLAILSYIVSNI"
     gene            complement(934..>2128)
                     /locus_tag="PLU08_05020"
     CDS             complement(934..>2128)
                     /locus_tag="PLU08_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71076.1"
                     /translation="YIHMSRKFKQKPKKVKAEKVKREPDMRKRAYLAMLFNNRAAFDG
                     GRREPWWVAVLFFIASIVIALVPAMVQVGKTKGSDIFKGPLYHTDVAFTKFVETLEEK
                     DADLTVVSENDENIFKASPEFVNLVANKAFTLTDGATNEVVPYYSFAQKRIVYTRDEN
                     NAVVTNEVDFEYLRVYYTGDIQSSFLLEGKVYSGDAFLALKLLSLKEEDAVGNVTSHL
                     IIGRKALYTRLYNPTAINKPGNPALVFEGRTNSLPVGMNIRDFGKVSKDGVPLAKTDI
                     DYTDKVMENFGHMQDLGYKEVKVRTFWFQTGIYAAIFSIIGLVMGLIIFISTRGKMNP
                     NRYLKFGESLKIGAWLLPAPALITLVLGFILPAQYFQMIFIMTLGMRSVWLTMRTLNP
                     NMPQQ"
BASE COUNT          713 a          372 c          377 g          666 t
ORIGIN      
        1 gagcgattat ggcgattaag ggcatttttg tcacgaatat cagtcataat agcccaaaat
       61 ttcgcattca agttgatgaa tttttaaaca aagctaaact tataccaaat cttgaattaa
      121 ttgaagttaa aaatcaacat gtcttaagtt atttaaataa taatacacct gacttttgtc
      181 ttttttggga caaagatata tatctagcaa aacgtattga aaatagcgga attaaagttt
      241 ttaataatcc tgaaacaatt tatctctgtg acgataaagc attaaccgct gcggagcttg
      301 ctaaagcgcg tgtagcgcaa ccacgcttct ttgtatttcc gcttcattac tggggcaata
      361 ttattgaata ttatgaagag tataaagaag agttacttgc attatctttt ccccttgtca
      421 ttaaagaacg ctttggttca tttggtgatc aagtttattt agcgcatagt gaagaagagt
      481 taaaaaagat tataaaagag cacggcacaa aaggactttt ggctcaagaa tttgtcagcg
      541 gcgaaaaagg gacagattac cgtgttaatg ttgtgggaga taaggtaata tttactgtta
      601 aaagagtcaa tgacaaagat tttagaagta atattaatca aggcggcact gcttcattag
      661 taaaagccac tagcgaaata gaagatttag cgattcaggc aacaaaagca ttaaaaggtc
      721 atttcgcggg tgttgatatt atgcttgacg ctaaaggaaa accagtagtg atcgaagtaa
      781 attcaaatat gcgcacggtt gcagttaata aagtaagtga tattgattta acactagcga
      841 tattaagtta tattgttagt aatatttaat gtacataaaa gttaaaaaaa tcaccttgtt
      901 tttagcaagg tgaattttta ttaattctta atgttattgt tgcggcatat ttggatttaa
      961 tgtccgcatt gttaaccata cactgcgcat accaagcgtc ataatgaaaa tcatttggaa
     1021 atattgcgct ggtaaaataa agccaagcac gagtgtgatt aaagctggag caggaagtaa
     1081 ccacgcacca attttaagtg attcaccaaa cttaagatag cgatttggat tcatttttcc
     1141 gcgtgtagaa ataaagataa ttaaacccat aacaagacca ataatagaga agatagcagc
     1201 gtaaattcca gtttgaaacc agaaggtccg tactttaact tctttataac ctaagtcttg
     1261 catatgacca aagttttcca taactttatc agtgtaatca atatcggttt tggcaagtgg
     1321 gacaccatct ttactcactt taccaaaatc acgaatattc ataccaactg gtaatgaatt
     1381 tgtccgtcct tcaaacacta aagcagggtt accaggttta ttaatggccg ttggattata
     1441 aagtcttgtg tataatgcct tccgcccaat aattaaatgt gaagtaacat taccgacagc
     1501 atcttcttcc tttaatgata aaagtttaag cgctaaaaaa gcatctccac tataaacttt
     1561 tccttcaagt aaaaacgaac tttgaatatc accagtgtaa taaacgcgta agtattcaaa
     1621 atcaacttca ttagtaacga cagcattatt ttcgtcacgc gtatacacta tacgcttttg
     1681 cgcgaaggag taataaggaa caacttcatt cgtggcgcca tctgtcaaag taaaggcttt
     1741 gttcgctact aggttaacaa attcaggact agccttaaag atattctcat cattttcact
     1801 tacaactgta agatcagcgt ctttttcttc aagtgtttct acaaacttcg taaacgctac
     1861 atcagtgtga tataaaggcc ctttaaagat gtcgcttccc ttagtcttac caacttgtac
     1921 cattgctggc actaaagcaa taacgattga agcgataaaa aagagcactg cgacccacca
     1981 cggttcgcgt cttccgccgt caaatgcagc gcgattatta aaaagcatcg ctaagtaagc
     2041 gcgtttacgc atatcaggtt cgcgttttac tttttctgct tttacttttt taggtttttg
     2101 tttaaatttt ctactcatat gtatatac
//
LOCUS       DAOSTA010000075         1924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_54817_length_1924_cov_11.015516, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000075 DAOSTA010000000
VERSION     DAOSTA010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54817_length_1924_cov_11.015516"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1340)
                     /gene="metK"
                     /locus_tag="PLU08_05025"
     CDS             complement(159..1340)
                     /gene="metK"
                     /locus_tag="PLU08_05025"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010288382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOQ71077.1"
                     /translation="MANYRLFTSESVSEGHPDKVCDQISDAILDAVLAINPNARVACE
                     VFATTNFVLISGETSLTKEEEPDYENVAREVIRDIGYTNPKLGFSADAAEIVIKMNQQ
                     SSDIAQGVTQIKPEEQGAGDQGIMFGYATKESAGLMPLAIVIAHKLMRVATKMRKDGE
                     FPGARPDMKSQVTMRYYEDGSSAIDTLLMSVQHDEDVNLEEFKKFIHQKIMIPVVESF
                     GKNTDFKYLVNPTGRFVIGGPAGDTGLTGRKIIVDTYGGYAKHGGGAFSGKDPSKVDR
                     SAAYYARYIAKNLVESDVCDEVEIQLSYAIGVAEPLSVAIDTFGTGKVADEVILAAIS
                     KYFDCRPGMITQEFSLRKPKFKYRDLAALGHFGRPDINIPWEETNKAQALKSYFKKYL
                     V"
     gene            complement(1540..1728)
                     /locus_tag="PLU08_05030"
     CDS             complement(1540..1728)
                     /locus_tag="PLU08_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71078.1"
                     /translation="MDKMTFIDRTLCKLLFKMLAKKEKDKLEVWEKALLAAGNRVVDW
                     TNERYLAPITKYLANLNQ"
BASE COUNT          575 a          397 c          304 g          648 t
ORIGIN      
        1 gaaagtttct tttctaacgc atttcttatt gcgtcggtga cgacgatgtt tttgccaaca
       61 atttgatatt tcatttatcg tttcctttct atcaagagat ggcttatgta ttcttatttt
      121 aacatgagaa tagtaattta taaagaggaa gttttaattt agactaaata ttttttaaag
      181 tatgatttta atgcttgtgc tttattagtt tcttcccacg gaatattaat gtcgggacga
      241 ccaaagtgac caagcgccgc aaggtcgcga tacttaaact tcggctttcg taagctaaat
      301 tcctgagtta tcattcctgg acgacagtca aaatacttgg aaattgccgc taaaattact
      361 tcatccgcga ctttaccagt accaaatgta tcaatagcaa cacttagtgg ctccgctact
      421 ccgattgcgt aacttagctg aatttcgact tcgtcacaca catcgctttc cactaagttt
      481 ttagcaatat aacgcgcgta ataggcagcg ctgcggtcaa ctttactcgg gtctttaccg
      541 ctaaaagcac cgccaccatg cttcgcgtat cccccgtaag tgtcgacaat aatcttgcga
      601 ccagttaaac cagtatcacc agcaggacca ccaatgacaa agcgtcccgt tgggttaact
      661 aaatatttaa agtcagtatt tttgccaaaa ctttccacta ctgggatcat aatcttttga
      721 tgaataaact ttttaaactc ttcaagatta acgtcttcat catgttgcac actcattaat
      781 aatgtatcta ttgcagagct cccatcttca taatagcgca tcgttacttg cgatttcata
      841 tcaggtcttg caccggggaa ctcaccgtct tttcgcatct tagtcgcgac acgcataagt
      901 ttatgagcaa taacaattgc taaaggcatt aaccctgcac tttcttttgt agcgtaacca
      961 aacataatcc cttggtcacc agcgccttgt tcttccggtt taatttgtgt taccccttgg
     1021 gcaatatcac tactttgttg attcatttta atcacaatct ccgccgcatc agcgctaaaa
     1081 cctaacttag gatttgtgta gccaatatct ctaattactt cacgagcaac attttcataa
     1141 tctggttctt cttcttttgt tagtgaggtt tcaccactga ttaagacaaa attagtggta
     1201 gcaaaaactt cacacgctac tcttgcgtta ggattaatcg ctaacactgc atcaagaatt
     1261 gcatcactta tttggtcaca aactttatcg ggatgtccct cacttactga ttcactagtg
     1321 aataaacgat aattagccat aattttatcc tccgttaatt aaaattactt tcgcaaggaa
     1381 agtaatttaa cactttcctt atcgttacaa gatttgcctt tagcgagcac ttacttatgt
     1441 tcataagagt tgctactaca tcaatgccac aatgtagttc ttgataaggt taaagtaagt
     1501 ataacaaaat atatagttga aataaaataa tacgaatgct tattgattta gatttgcaag
     1561 atacttagtg attggcgcta aataacgttc attagtccag tccactaccc tattacccgc
     1621 agcaagcaaa gctttttccc atacttcaag tttgtctttt tcttttttag cgagcatttt
     1681 aaataataat ttacataagg tgcgatcaat gaatgtcatc ttatccatgt tccacgctcc
     1741 gcgcaaatag aaacacggta tatgtttaag gtcatctttt aaattatggt ctttaagtgt
     1801 ttgaaaagct ttttcatcaa acggagaagc accaacacaa aaaattaata cttttttatc
     1861 gcttaataat ttaggataga tgaataatta aaattaacaa gattaagtgt tttaatgatt
     1921 ttat
//
LOCUS       DAOSTA010000076         1652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2227
            NODE_68570_length_1652_cov_10.785848, whole genome shotgun
            sequence.
ACCESSION   DAOSTA010000076 DAOSTA010000000
VERSION     DAOSTA010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864294
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:45:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,009
            CDSs (total)                      :: 964
            Genes (coding)                    :: 955
            CDSs (with protein)               :: 955
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68570_length_1652_cov_10.785848"
                     /isolate="AMR_MDS_2227"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PLU08_05035"
     CDS             complement(<1..727)
                     /locus_tag="PLU08_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71079.1"
                     /translation="MKLRKLKAVPLALVTLFTLTSCKLIDNIIGSILVNTVMDLPVLI
                     NRLDLMPSAENTNPTFVPFENPPSSEANRAELNYLKNVLVVPRDLETVVFNKTIKLHF
                     DFEFSGEGATNAFHVIDVTESDLPENFRFDTSAFPVPITYKLALIVPVGESTILEGVT
                     NINQIQTVLEDTPRDELVELIDQEPLNATMKVIARSAGKSKSKNFYFNLTKPLFGAMV
                     LEEVFKSGYLINLYRIHGFNQENN"
     gene            complement(750..1493)
                     /locus_tag="PLU08_05040"
     CDS             complement(750..1493)
                     /locus_tag="PLU08_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ71080.1"
                     /translation="METKKKRTINIILKISIIVAAIIGIISQQLHGLLIYTGHVPFMY
                     FTTQSNILVAVCMGILLYYDLTEKEVPRWVLIFQHITVTAVTLTFLVFAIFLGPFIPD
                     LSVGYFYSVQNLTLHNIVPILAISQYLYDDNRDIPKYAKYLGLSSGFIYVIFVYIVHL
                     CGATFGATEFPYFFLDYKEFGWLTINEGKIGIIYWWIVIILLLLGISALLYYLKKKSA
                     ERKNIPVAIFNSLIILSIIFITINIIIKI"
     gene            1576..1652
                     /locus_tag="PLU08_05045"
     tRNA            1576..1652
                     /locus_tag="PLU08_05045"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1610..1612,aa:Thr,seq:cgt)
BASE COUNT          570 a          275 c          290 g          517 t
ORIGIN      
        1 cattattttc ttggttaaaa ccgtgaatac gataaagatt aattaagtaa ccagatttaa
       61 atacttcttc aagtaccatt gctccaaata atggtttagt taaattaaaa tagaaatttt
      121 tgcttttact tttaccagcg gagcgcgcaa tgactttcat cgtggcgtta agtggttctt
      181 ggtcaatcaa ttctactaac tcatcacgtg gtgtatcttc taaaaccgtt tgaatttgat
      241 tgatattagt aacaccttca agaatcgtac tttcgccaac aggaacaatc agcgctaatt
      301 tgtaggtgat gggtactggg aaagcgcttg tatcaaagcg gaagttttcc ggtaagtcac
      361 tttccgttac atcaatgaca tggaaggcat ttgttgctcc ttcaccacta aactcaaaat
      421 caaaatggag tttaatcgtt ttattaaaga cgaccgtttc aagatcgcgc gggacgacta
      481 atacattttt taaatagtta agttcagcgc gattagcctc actacttggt ggattttcaa
      541 atggcacaaa tgttggatta gtgttttctg cacttggcat taaatcaagg cgattaataa
      601 gcacaggtaa atccatcacg gtattgacaa gaattgatcc gatgatatta tcaattagct
      661 tacagctagt aagagtaaat aaggtgacaa gcgccaatgg gactgctttt aatttgcgaa
      721 gtttcataat agaaccccct aaaccttctt tatattttaa ttataatatt aattgttata
      781 aaaatgatag ataaaataat tagactatta aaaattgcaa ccggaatatt tttgcgttca
      841 gcactttttt tctttaagta atataagagt gcactgatcc caagtagtaa taaaattatt
      901 actatccacc aataaataat gccgatttta ccttcattta ttgttaacca tccaaactct
      961 ttatagtcaa ggaagaagta aggaaactct gttgcgccga aagtagcgcc acaaaggtga
     1021 acaatataca caaaaattac ataaataaat ccgctagaaa ggcctagata tttagcatat
     1081 tttggaatat cacgattatc atcatataaa tattggctga tagcaagaat cgggacaata
     1141 ttatgtaatg ttaaattttg cacggaataa aaataaccaa cgctaagatc aggaataaat
     1201 ggaccaagga aaatagcaaa cactaaaaag gttaatgtaa cagcggtaac tgtaatatgc
     1261 tgaaagatta aaacccagcg cggaacttcc ttttcagtta agtcgtaata tagtaaaatt
     1321 cccatacaaa cggcgacaag aatattactt tgggttgtaa agtacataaa tggtacgtga
     1381 cccgtgtaaa ttaataaacc atgcagttgc tgtgatatta ttccgataat tgcggcaacg
     1441 ataatactaa tctttaagat aatgttaata gtgcgttttt tcttagtttc cataaaaaga
     1501 ttatactaca aatcaagata ttttcttgat gtttttattg caatattgtg ataaagtcga
     1561 tatgattaag actaggccga cgtagctcaa tctggcagag caactgaatc gtaatcagta
     1621 ggttgcgggt tcaattcctg tcgtcggcac ca
//
